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    <name>A0A287H305_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123424544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="161"/>
        </location>
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            <begin position="642"/>
            <end position="664"/>
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            <end position="140"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0105680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
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    <sequence length="806" mass="89634" checksum="704C9ACE915D78E4" modified="2017-11-22" version="1">MDCHLATTSCMVHRIDSTLRRPAKFKKTRVISDIYSDSYELDLTTLKPVKTEMIDSEQVASPSPNLGEQDVHNASLMDLKARCKAKNKKAPKTISEESGTKNRSQCGDFGDERLKEEVDLDEPLIALKQKKEKIPPRKAKGKMDASSSPHATKPVDTSPKRDEISHVQHFLFESTLHDPMTEKLETRTVDQEHCRIAIEHNEEVVGEDICCAEMENAVFHTRDHLSVVLHQFPIEDNGCGQQPGFITQPIEQDVSDAKAHLHDNVEQKVMDHRFSSLDPIDQVCNHQRSLDDTSISDVNKSSVGNELPLSSVSRSCDDHTDNNEYKYPEIVQVNTSESIKPVEESSPIDELTADVRCTLVVMQPDSCGSTDKICTSFEEVVQMPVEVQPDSLVCHDVKTKDILLHMNVEQAAIGYSFSYDKTLDLAHTANFGAQDGQLENVVYDALNNHVQQECSETKTSVGVPDTVVILSPPTVANVSHDGHLLLANMDESSKDMNQQSGRMNVDICRSVDDQEPREAYVVQHKSFQACVNMDKTGCAISDSFSNPEETQKISAEASSSTPTCLGTDGQTRASEFIIDEGSIEVHTPKKLLSKRKTMSPTCQEKFCNAWTGIDLYGVQRPKTKINLEDRSMLTTDRKLKNRTSSSFTSKGVVKSTESQSPQLGSSSVLLDTEKVVDFSQRQMHDIENIAANLISSLKHMRSLVNETLSSEAHSLLPSFNTAEMRAASENVIDVERTTRKWLTIMNKDCNRFCKILTLDGKKAVPHPQVPRKRRKITFADEAGGTLCYVKVFSDGQTSPSACQGEL</sequence>
</entry>
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    <accession>A0A8I6WDA7</accession>
    <name>A0A8I6WDA7_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">NB-ARC domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDA7"/>
    <dbReference type="SMR" id="A0A8I6WDA7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010020"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010020"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF64">
        <property type="entry name" value="OS12G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="1">
        <location>
            <begin position="20"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="184" mass="20856" checksum="922F3E72C4AFD28F" modified="2022-05-25" version="1">MAMLRETPQGDAPRSSPCYSVIGIYGVAGSGKTTFAGYIRDYINEECKEDKHFDTIMCIHVSETFSVDDIFHEMLKDITKDRHSNISDREELEEKLKKALRGKRFFLILDDLWVKNKHDSQLEELISPLIVGAKGSKILVTTQTKDAAGALCAKELIKMPGMDEDQYLKMFMHYALDGTSFAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDB4</accession>
    <name>A0A8I6WDB4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metallothionein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123412331</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Metallothioneins have a high content of cysteine residues that bind various heavy metals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallothionein superfamily. Type 15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961225.1">
        <property type="nucleotide sequence ID" value="XM_045105290.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8I6WDB4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061530"/>
    </dbReference>
    <dbReference type="GeneID" id="123412331"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0048910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0048910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0048910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0061530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412331"/>
    <dbReference type="OrthoDB" id="678987at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000347">
        <property type="entry name" value="Metalthion_15p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33543:SF15">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33543">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01439">
        <property type="entry name" value="Metallothio_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="74" mass="7441" checksum="86EDA6F8F6F8B824" modified="2022-05-25" version="1">MSCSCGSSCGCGSNCNCGKMYPDLKEKSGTTMQATVIVLGVGSAKVQFEEAVESGEAAHSCSCGASCKCNPCNC</sequence>
</entry>
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    <accession>A0A8I6WDD4</accession>
    <name>A0A8I6WDD4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDD4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062490.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049660.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062490.1">
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    </dbReference>
    <dbReference type="OMA" id="CDHLFHW"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000793">
        <property type="entry name" value="E3 ubiquitin-protein ligase SGR9 amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    <dbReference type="InterPro" id="IPR051826">
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="OS05G0408800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="216"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="289" mass="30353" checksum="1C4AFF9A67789325" modified="2022-05-25" version="1">MEASHAHSPTLSSSETIMAALLSSMSVQFPSLVRAIAADARRQRGRLAFLLLSPPHFARALARLRSMPLPAKAALLGRVLLRSLLMLLPALSPDGSHHLLRLPTPDLDAALLLVAMCDSYSPAASSASPVDWHALLVDDAVRNALSISGLGGTPWATVAPYLDAAAKCRRFADVVSEDRAVAGAGGGSSKDGVGRGGASQATVLALPTTPGDGTPCAICREEMAPGRGGVCGLRPCGHRFHWPCALRWLARRNTCPCCRAELPAEDALAETRRLWRAVERMAGDSGVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGD6</accession>
    <name>A0A8I6WGD6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGD6"/>
    <dbReference type="SMR" id="A0A8I6WGD6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001660"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001810"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0769120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0769120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043264836" description="Protein kinase domain-containing protein" evidence="10">
        <location>
            <begin position="28"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="380"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0769120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="451" mass="50837" checksum="7C983906BDF1B6B5" modified="2022-05-25" version="1">MGNPGAFHHSITLQSLTIFSLVAVLAADHVQGRDDGCTPFSCGHLQDIRPPFRRRGDPLECGVEAYELGCTSSKATIHINKGTYYVTAINYTSSYFWVMDPNFDTISSCPLPLWNHLPYFRYFHYFEYSYTLGGIDSVSPPGFRYLVTQSYYSACFANCSRAVAKNSAYKPVACLSAKNSHVYVWVSYYGCMVEDLEPYCGYLANIPFGNEYYSDWLQLQNASYADIIQVISKGFTVQFPVDTTGPSASMKLRKNINLCLNNSISYFKEQSSGAGIVNWAHVFFWSEVHFLECVTQLSDDHYFTTQFVLVVVTIVSAIAIPKFLFVLCRFLLAPLAVWIFLAYKYWKTRIIIDAVEKFLRIQQIIGPTRYAYTDIISVTSHFRDKLGQGGYGSVYKGVLLPGGVHVAVKMLEGNSNCNGEDFISEVSTIGRIHHVNVVRLMGFCSEEMRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WHA4</accession>
    <name>A0A8I6WHA4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123427557</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967570.1">
        <property type="nucleotide sequence ID" value="XM_045111635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WHA4"/>
    <dbReference type="SMR" id="A0A8I6WHA4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0189340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0189340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0189340"/>
    </dbReference>
    <dbReference type="GeneID" id="123427557"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0156800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0156800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0156800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0189340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0189340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0189340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427557"/>
    <dbReference type="OrthoDB" id="588330at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000037">
        <property type="entry name" value="acyl-acyl carrier protein thioesterase ATL3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
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    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0189340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="22824" checksum="DAA77A85A0DC637C" modified="2022-05-25" version="1">MYGQIWRLVPNACSPLPPIRAGAGRGGWPAGRPAPLRRTALASAATRGLCRPLAVSAQPASIDAGLRVDKFFEVEMKVRDYELDQYGVVNNAIYASYCQHGRHELLESVGISADAVARSGESLALSEMHLKYFAPLRSGDKFVVKVRLASIKGIRMIFEHCIEKLPNHELIAEAKATAVCLNKDYRPTRVSPEFLSRLQLFSSVDNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIC3</accession>
    <name>A0A8I6WIC3_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIC3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0133170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0133170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0133170"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0109790.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0109790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0133170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0133170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0133170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0133170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="145" mass="15996" checksum="A0BA02E84A908A06" modified="2022-05-25" version="1">MTEEEEARLVQLIMEDSMNTYDERQWVGLEEMMALSAAGDVAIPELEMAAAVTVKEEVHEEQRVVAFPPNLVGQRWTWSCTATEMAQGVGAEPWCPTPPRSPERESSPCGQVVQAPPTFEGPSTHPSTMPPYIDLTSDDDDNSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJ52</accession>
    <name>A0A8I6WJ52_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0073090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0073090.1.CDS.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0088860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088860.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="71" mass="8264" checksum="312E0923F325CDC2" modified="2022-05-25" version="1">MPYLGKSCGLSSTLYAVNQHMAWKWHIYKVYHLALLFHLPRTMPGKEIGYHVFNPWRRVPLDMGSCDLGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMQ6</accession>
    <name>A0A8I6WMQ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123429918</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMQ6"/>
    <dbReference type="SMR" id="A0A8I6WMQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0114810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0114810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0114810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0139370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
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    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
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    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
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            <begin position="1"/>
            <end position="22"/>
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            <begin position="28"/>
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            <position position="115"/>
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        <location>
            <position position="119"/>
        </location>
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            <position position="123"/>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU361120"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139370.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="306" mass="34123" checksum="E231CE4DA348FCBF" modified="2022-05-25" version="1">MGPWRRPCVGALLACAAIAASCCCFQLQGADAAASPSFGDNFEITGAKDHVKTSPDGQTWYLSLDNKTGVGFQTKQKYLFGWFSMKLKLVGNDSAGVVTAYYMCSDLDAAPERDELDFEFLGNRTGEPYIIQTNVYRSGVGGREMRHSLWFDPTADFHSYSILWNPKQIVFFVDKVAIREYRNSAKPNKFFPIMKPMYVFSSIWNADDWATRGGLEKTDWTKGPFVSSYSDFTADACAWPSGPAPPACAAATGDSWWDQPPAWALDDGQRRDSGWVARNLVIYDYCGDRKRFPTVPEECALRTTTS</sequence>
</entry>
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        </recommendedName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0020680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="453" mass="51200" checksum="89776D8F1EB0BDF9" modified="2022-05-25" version="1">MGACSLVRMMSLLQLDLRTLRTLLSLSSTPSSQGKFRKNPPLMLMETETVVVETLPEPPLMETETVVETLPELPLDVLMDILSLLEIPDLIRASSVCSFWCSAYSSLHSQLAQYKRPQTPCLLYASESDGENVACLYSLAEKRVYKLTLPDPPIRSRHLIGSSHGWLVTADDKSELHFLNPITGQQIALPSVITIEKVEPILDSAGTVNKNKMWDLTPDGYPSQLSDCLCIRAFVFPDPPTGSYIVVLIHNPEDYLSFARVGDFKWTSLPGKDYQQCIHMDGLLYAFTYTGGVYTFDLTGPTITRNTIADEVKNYISATDGYMYVVQAPWGDMLQVCRVIECIEGFIKTKKLMLYKADMAAKELVEMPGLHDHVLFLGRNQPQCLSVEEYPQLKTNCVYFTDDMTYISKYKNDDRDIGILNLENDSKEEIVSQLWCNWPNPIWITPNLTRMNM</sequence>
</entry>
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    <accession>A0A8I6WNU8</accession>
    <name>A0A8I6WNU8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNU8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0230300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0230300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0191050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0191050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0191050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0230300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0230300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0230300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="13351" checksum="4A06A4A66CA6B4B0" modified="2022-05-25" version="1">MRWCGVFEGMAAPCCQHRPRMAGSGGWCRCQCQRVRRAGADGRRWSKQGTDTPTASHSHLTASKDGYRVQVQMRRIPFNPNHDEAVVSLEVFRSTTQLTRTFPACSTEVWKITLESHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WP30</accession>
    <name>A0A8I6WP30_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123426828</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="17649" checksum="248B3D4AE5C84359" modified="2022-05-25" version="1">MNGYRNLASSPPAPSGGEPEPEPAPSPATAKEGKWEGAAVGAATMVRNLSSASHRFAAVERSKSTSGRRFQAVVRRAFSMRRQPASSLSEGYWRIHDGLDDGDDGAEAPEEVDEEKKRQAEPEVPGDAAASNGEVEKEESEKKKKKRGHIFKACKKLLGFKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X222</accession>
    <name>A0A8I6X222_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase A1</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123440044</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acylhydrolase that catalyzes the hydrolysis of phospholipids at the sn-1 position.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044972574.1">
        <property type="nucleotide sequence ID" value="XM_045116639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X222"/>
    <dbReference type="SMR" id="A0A8I6X222"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276230"/>
    </dbReference>
    <dbReference type="GeneID" id="123440044"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0228840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0228840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0228840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0276230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440044"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000065">
        <property type="entry name" value="Phospholipase A1-II 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033556">
        <property type="entry name" value="PLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31828:SF8">
        <property type="entry name" value="PHOSPHOLIPASE A1-II 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31828">
        <property type="entry name" value="PHOSPHOLIPASE A1-IIGAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035167596" description="Phospholipase A1" evidence="7">
        <location>
            <begin position="22"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="8">
        <location>
            <begin position="148"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="416" mass="45353" checksum="DC8D0DC053F3FA87" modified="2022-05-25" version="1">MYHYLQLSVLLVTILSDVAIAQRWREISGGNQWSGLLDPLDIDVRRDVIRFGELAQATSDAFITDPASPYAGACRYSPASFFSKVQASDPGAYRVTRFIYATSSARLPDGFMARPVPAGAWSTESNWMGYVAVATDRGAAALGRRDIVVAWRATKRVTEWASDLDFALVPAAGIVGPGRGWSQPYVHRGFLSVYTSKNSTSRFNKRSAREQVLTEVRGLLDTYKKENCSISITGHSLGGALSTLNAIDLVANGFNVRGPSRSPVPVTAIHFGGPRVGDEQFKKAFHSMAGLSLLRVRNVPDIVPTILPPVFYSDVGVELLVDTRKSPYLKEKAGPAQWHNLEGYLHGVAGTHGAGDAAGFSLVVDRDLALVNKEEDALKDDYPVPAMWWAENNKGMVKNATGHWVLHDHEEGNLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X285</accession>
    <name>A0A8I6X285_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glycosyltransferases</fullName>
            <ecNumber evidence="13">2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123406394</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of glucuronoxylan hemicellulose in secondary cell walls.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044955846.1">
        <property type="nucleotide sequence ID" value="XM_045099911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X285"/>
    <dbReference type="SMR" id="A0A8I6X285"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013800"/>
    </dbReference>
    <dbReference type="GeneID" id="123406394"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0010500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0010500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0010500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0013800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406394"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000084">
        <property type="entry name" value="Glycosyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF59">
        <property type="entry name" value="BETA-1,4-XYLOSYLTRANSFERASE IRX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="12">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="41231" checksum="7D7057C6FDCCC6FE" modified="2022-05-25" version="1">MGTGAVAPERPKQRRGGHLWKRAVLHFSLCFVMGFFTGFAPSSSSSWRPGGGGGTPPPVLAAEQLAASRVAGNRDQRISLAPPSPEGAAAAGGGGAVVDLDDDEESGPRRMLIVVTTTRSGAGERRRRRPELLRLAHTLRLVRPPVVWVVVEPAADAPATAEVLRGTGVMYRHLAFRPEENFTTAAAEAHAQRNAALAHVEKHRLSGVVHFADAAGVYDTHFFDEIRQIEAFGTWPVATMSAGEKKVVVEGPLCSASKVVGWFSRNFNDGTTRSVTYNTEVDLNPAGAAGTRAHTIDVSGFAFNSSILWDPERWGRPTSLPDTSQDSIKFVQEVVLEDRTKLKGIPSDCSQIMVWQYDVPGSASAPKTKSKTATPKTHHRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2Q4</accession>
    <name>A0A8I6X2Q4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2Q4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0216290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0216290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0216290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0179800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0179800.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0179800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0216290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0216290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0216290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0216290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="17880" checksum="0491B4B0456BF5A2" modified="2022-05-25" version="1">MAQQLKDSIFFVFSEGSRFWFAMCLSLEGVQCKTVYCVRLTDLMANLIQDYGDTSRLCVYLWLAFLEREMDSAATSLHDKSRFPDLFLAFRKEVEKFLKYAEADPGFNNRPLIKFKGSNDGYCLGALVGQSLFIIKYSCVLVRKALMREANYTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3D2</accession>
    <name>A0A8I6X3D2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123439215</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044971890.1">
        <property type="nucleotide sequence ID" value="XM_045115955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3D2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223360"/>
    </dbReference>
    <dbReference type="GeneID" id="123439215"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0185320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0185320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0185320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0223360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223360"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439215"/>
    <dbReference type="OMA" id="TICFEHL"/>
    <dbReference type="OrthoDB" id="660773at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF81">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="32"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="86"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="300" mass="33160" checksum="40D3C7983EB15AF6" modified="2022-05-25" version="1">MNGSSSNKRSAEAIREGDTWMDVRILKETLDCTICFEHLTTEIYQCSVGHFVCSSCRDKILDKKCPTCSIKTSFNRCFGMEHVVQSVAFPCSNAKYGCREGHTYYQKERHEQVCTYAPCFCPAPDCSFAGPTSELLDHLSIYHVSPCTDLLDSGTVSLSLEPGIHVLSSRSKTTNYFFLLNMEPEHLGYAISVVCIQPKATEPKFTCNMNYYCSVTGYCQSSSCHIKSSTLSDGLPTDYDFILPKGKVSVGDQTVVMLRITIHEASSVGRSRVQGNGPTSAPLTPFRCCSDDDADILFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6R6</accession>
    <name>A0A8I6X6R6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6R6"/>
    <dbReference type="SMR" id="A0A8I6X6R6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0061670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="entry name" value="OS05G0355200 PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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            <begin position="379"/>
            <end position="413"/>
        </location>
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            <begin position="414"/>
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            <begin position="177"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061670.1"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="704" mass="77563" checksum="6C2AC190D52D0712" modified="2022-05-25" version="1">MFTTVHQNGLTLLRNIMVFSGPSPVERVGTTAKPHHAMLTRRYSMAFKPKHASSACLAAAVHGDLQIAAAALLAAAGALRSDGAAVPLPGAPHPRPHRHIYRPHGPLPPLLPLPCRPPHLPPPLPAPHLTSGVRSNLWGEDAGAGLLPGRALPLLSARSRSLPAASPAWGTDAPFRLAGSRRLSTSTSSASEDDDEGPSTPQAPSSHPEHVARVCAAIADVITSGADANLEAALSALSLPLSEPLVVAVLDRFKHAHKPSRRFFQWAAASGGFAHSPITYCKMLQILGKARQFETMVALVQEMGKAGALCMDAFKIAIKSFAAAGEIKNAVGVFEMMRVHGFDDGVESFNCLLFALAHEGLGKEAAQVFTRMHDRYTPDLGSYTALLLAWCNVRNLVEAGRVWNEMLEKGMKPDVVVHNTMIEGLLRGQRRPEAVKMFELMKAKGPPPNVWTYTMLIRDHCKRGKMDMAMRCFEQMQEDGCQPGVATYTCLLVGYGNAKRMDRVAAMLEEMSQKGCPPDGRTYNALIKLLTNRNMPDDAARIYKKMISKGLQPTIHTYNMMMKSYFLGGRNYAMGSAVWEEMYQKGICPDVNSYTVFINGHIRHGRPEEACKFIEEMIQKGMRPPQIDYNKFAADFSKAGKPDILFELAQKVKFTGKFDESNVFHQWGERMRSRVKQTVPDQARSRTFYLQLGKWMAGCYLTWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X835</accession>
    <name>A0A8I6X835_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X835"/>
    <dbReference type="SMR" id="A0A8I6X835"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0358100.1">
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0430800.1"/>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
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    </dbReference>
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        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GNK2"/>
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        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035151832" evidence="19">
        <location>
            <begin position="21"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="29"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="142"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="357"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0430800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="687" mass="74800" checksum="08D4A056A49EC38E" modified="2022-05-25" version="1">MDSSLVILLAVVASLGAAAAIGKQDKFLAPVNPSCSTTGNYTDGSQFKKNLDELLIALPVNASRNWGFYIGMAGDSGSPDQVFTLIMCYADYGAAECLECLNGAPAGIMTLCPGSRDVRVAYDACIFRYSPASPFASMADLDTPFYVKYTTPIPVDPAAMARAWLPLMVDLTGQAAGSLSRAANGSTPYDASWRVFGLAQCTRNLNTSECSRCLSSLVGKLPELFRNETGGAVKAYSCYVRYQIGSFDITLPPVAEPPPPSSPKPGETSSSSRTRLLIGISIGTVSFLIILVGFLVCLLLRRRQKHPITVAKKAKGQEPEDGKFSDGDDPAMEDDFEKGTGPKRFHYGELAIATDNFSDEKKLGEGGFGSVYRGYLEEMNLDVAIKRVSKGSKQGKKEYASEVTIISRLRHRNLVQLIGWCHGGGELLLVYELMPNGSLDTHLYGGKNAAVLPWPARHEIVLGLGSALLYLHQEWEQCVLHRDIKPSNIMLDASFTAKLGDFGLARLVDHSRGSHTTVLAGTMGYMDPECMTTGRTSAESDVYSFGVVLLEIACGRRPLAMAEEEQMVHLAQRVWALYGVGRVLDAVDARLLGEFDGEEMERVVVVGLWCAHPDRSLRPSIRQAVGVLRREQPLPTLPERMPVATFVYVPLLVMVQVPRCLLVPPALAAAAELTRRRRSRCRCEATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XB90</accession>
    <name>A0A8I6XB90_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XB90"/>
    <dbReference type="SMR" id="A0A8I6XB90"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0081570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0081570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0081570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0099010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF39">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="313" mass="33511" checksum="44812AFC93FD063B" modified="2022-05-25" version="1">MAAVTLGTDTDGSILCPSSFNSVVGIRPTLGLTSRAGVVPITPLQDTVGPICRTVSDAVHVLDAIVGYDELDAAATGAASKFIPPGGYTQFLKKDGLRGKRIGVLRGFFRHAGTQPLRVYEQHLATMRKHGAVVVDNLHVAANPTALLDDISSNEGIAMQAEFKLSINAYLADLLYSPVRSLADIIAFNNKHPVEERMKDFGQDDLIAAQNTTGIGPVERVAIRRLKELSANGLQKLMKERQLDAIVTPSSDASSLLATGGHPGIVVPAGYDEQGVPFGICFGGLQGYEPRLIEMAYAFERATKVRMPPMFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDC9</accession>
    <name>A0A8I6XDC9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDC9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0349640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0349640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0349640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0291710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0291710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0291710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0349640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0349640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0349640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF184">
        <property type="entry name" value="DUF6598 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="1">
        <location>
            <begin position="7"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0349640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="205" mass="22163" checksum="B2C5DD8E0EEA4EF1" modified="2022-05-25" version="1">MQSFTDLVLTGPYVAISADGESFAFKVNISGSDEFDDTNEGTLWEFIVDDVDNVVESSIATDDGDILVTYAVLGNAVEANVQVYLVHFRDTTNTSTHVHGKISVHIPLYGADYKVWLLSCQDDEKVELIPSSAGSIVPLARSIVAVPIGSPLMVKVSLLATPTPTNQDSTLFLQGNLNFSFGDQTLGFHTAIGKLMVRVDVTHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDE4</accession>
    <name>A0A8I6XDE4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDE4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0156730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0156730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0156730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0129100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0129100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0129100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0156730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0156730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0156730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF7">
        <property type="entry name" value="OS05G0589000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0156730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="265" mass="29639" checksum="12A59D142F7F893E" modified="2022-05-25" version="1">MSTTGCTQPSRPGIRSSPSASSENVRARNSGSSSRSAAPPPAAPQAGAANPLRFDARTIHFSVNAWVLVVVGLGMLPILPKHLADRACKLSLLGTVLSSGYSLYSTYGKPRELNMPAIQSWLQSVLGAKDFIHLMFSLLLVTSQLHLKIAALPVFCWALYHVARFLRRNFSRSSFYRGYLEEPCLWVETNNTTLSLLSSNAELALGFLLIISLFLWRRNFMQTFMYWNVLKMMYRAPVTSSYHQSAWAKIGRTVNPYIDRFRGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFB5</accession>
    <name>A0A8I6XFB5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123447319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979845.1">
        <property type="nucleotide sequence ID" value="XM_045123910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFB5"/>
    <dbReference type="SMR" id="A0A8I6XFB5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335910"/>
    </dbReference>
    <dbReference type="GeneID" id="123447319"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0280110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0280110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0280110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447319"/>
    <dbReference type="OrthoDB" id="1065058at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903229">
        <property type="term" value="P:xanthosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010237">
        <property type="entry name" value="Pyr-5-nucltdase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01993">
        <property type="entry name" value="Pyr-5-nucltdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725:SF81">
        <property type="entry name" value="OS03G0701200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01132">
        <property type="entry name" value="C1.5.3:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="272" mass="29664" checksum="69480FC841319177" modified="2022-05-25" version="1">MAFNSPFDCLLIDLDDTLYPGNTGIGPALRRNIDEFLVARFGLAADKAAALRADLFRSHGSTLAGLIALGHDVHPDEYHSYVHGRLPYDVIAADPQLAAALQSMPQRKILFTNSDRAHMRRSLERLGLDEALFDDVVCFETMNPHLFGEEAAEDDAADVDCPAVVLKPSVHAFATALRVAGTNPRRTLFLDDNERNIASGKALGLRTALVGKRVRSKEADYALETIGGLRRAIPEIWGGAADGELQPDHNVERNKSMRAELDAVIQPTSIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK21</accession>
    <name>A0A8I6XK21_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448782</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981720.1">
        <property type="nucleotide sequence ID" value="XM_045125785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK21"/>
    <dbReference type="SMR" id="A0A8I6XK21"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380730"/>
    </dbReference>
    <dbReference type="GeneID" id="123448782"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0380730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448782"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043554">
        <property type="entry name" value="KINB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316:SF4">
        <property type="entry name" value="BETA SUBUNIT 1 OF SNRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="296" mass="32036" checksum="9A548796BAFA8EE7" modified="2022-05-25" version="1">MGNASGREEDAAAPGEADAADVDVEDSSARGGFPPYGGGANNVRRACSVGVVGASGGPGSPPGSPGRSLSPRMFVPQTPVPPLQRPADITPVFNEILMNEEEEEFDGPPQKEIPALIVWTLGGKNVSVEGSWDNWKSRKPMQKSGKDHSLLLILPSGVYRYRFVVDGERRCFPDLPCETDAMGNAVNLLDVHDFVPESVESVSEFEAPPSPESSYSFQAPEEKDFAKEPPALPSQLHLGVLNSQNSEEVCARPQHIVLNHLFIEKGWGAHPLVALGVTHRFESKYVTVVLYKPIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLT9</accession>
    <name>A0A8I6XLT9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123397775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948241.1">
        <property type="nucleotide sequence ID" value="XM_045092306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLT9"/>
    <dbReference type="SMR" id="A0A8I6XLT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445910"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="11177" checksum="A8906FA868EF9386" modified="2022-05-25" version="1">MDRVMKMAPERGVVIFTLNSCCMCHTVTRLFTDLGVNALVHELDHDPKGKEMERALLKLLGKGPPVPAVFIGGKLVGGTNKIMSLHLGGELVPMIRNAGALWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="137" mass="16468" checksum="0F375CAB037882EE" modified="2022-05-25" version="1">MNFQDVHTLQQALDVAPPPRLNSTQDRAEHTALQRRLLVAQEDERVMAEWRRRHLEDVAYEQEYWARRREEDTRRRREERLDRRRRKALASAQADIVNAGGRSFFTEEDERWLVIWLSTSDDTNDDDNGADDWSDWN</sequence>
</entry>
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        <lineage>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF7913"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913">
        <property type="entry name" value="ALEURONE LAYER MORPHOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913:SF6">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495870.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="70038" checksum="78302D65CA629D49" modified="2022-05-25" version="1">MGHGRIRAEEEDGEMMGLDGGGGAEIHTAIILYNYYHRKMSPQLAFADAKRFFKCASLSVGEDLLAYLSMVHERENNSGKHAKLSITDKAALQACEIAEELDASKHSPDLTTWQISKVAVLLLDPTRKKCLVDYSADTKGIWSIIEKELDAPAGNSHSTNQPAGQESTAKGNVGASHTPIMLQRQAYSEVERRTGMKASNFRLVDETLAYSLSKERAATKVFIVEYGQTMKGNLEEMSLEELITSMTGPLFVNDPFPKTTSVVEYYHILPYKEILSQLLHRKWSSGSSPNEQSRRHGKGSFHSVIDENVEEQDENSTSKLQKRITKVSTPKQSKQAIGASKHKKSSKRKSEASMAAADACAESPDSEIPTIKHVIEVETSKPMTKSRKTEAAATAASGETKILQAGVQVEKNKTQEQSKCDNMPQDVFPTEAPHADVVKNRILECENMDLSGKPGGTTEYNDDDQIEDSLRSIQKIRDEILRKESILQERSAQCDMDIQTLLSEGKMTPKAVPIISKYKETCSDMTEVANSSCSGDGGQSITKRKRLREALLRHNSYEDLYEICRGAHWIFPRYTVVPSVSDGMYHASVRLRCPDFEMSITGGLRPTPHEAKCSAAANMILELHRKAEQEQ</sequence>
</entry>
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    <accession>A0A8I6XRQ3</accession>
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        <recommendedName>
            <fullName evidence="16">DNA 5'-3' helicase FANCJ</fullName>
        </recommendedName>
    </protein>
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        <name evidence="19" type="primary">LOC123397086</name>
    </gene>
    <organism evidence="19 20">
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        <lineage>
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    <reference evidence="20" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947706.1">
        <property type="nucleotide sequence ID" value="XM_045091771.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0503930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503930"/>
    </dbReference>
    <dbReference type="GeneID" id="123397086"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0503930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0503930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397086"/>
    <dbReference type="OrthoDB" id="19182at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA helicase activity"/>
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    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fanconi anemia group J protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
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        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF47">
        <property type="entry name" value="FANCONI ANEMIA GROUP J PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="33"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="821"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="969"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1345"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="977"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022485"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51193"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1373" mass="150372" checksum="5B1CE008373CE471" modified="2022-05-25" version="1">MGAAHDDGDFAATPAATPAPAPARGGGGVFQVAGLPVEFPYKPYGTQLAFMGRVIATLDRGRRQGRSHALLESPTGTGKSLSLLCAALAWQRHYPLRAPPAPAAADPLLHGGGFLPDDTQQQQQQPTPGVLEKAAAAAKKKKAPTIYYATRTHSQITQVVRELRKTPYRVRMAILASRKHYCVNKSACMTDSVDEECKRLLDDKVHGCLEFKNAQKLSRHPSLQTGGSYEVHDIEDLLKVGRQVKGCPYFAAQTMAEAAQLVFCPYNYLISPIVRRAMDINIAGSIVILDEAHNIEDISRDSGSVDVDEESLNSLAEELAKLATDQAVGKIYQPLHDIIQGLSNWIAAQEGDPRDNEFGRPASFWTGENAVKELQKAGITPAYFPILQECATKAVKAASDTESEGDHLTGRGSVTLESLFSSLSHFFGRNGRHLYDYQLAIQRLAHREGTSAFGVRNVMSLWCLNPAVVFQDIADITHSVILTSGTLSPMGSFASELGVQFDACMEAPHVIDADSQVFASVLSSGPTTHRLNASYKSADAYSFQDELGASIEEICRIVPGGALVFFPSYKLLDKLKVRWSQTGQWARLNAQKPVFVEPRGSTEELEPVLNGYYNAILGKVPLKKGRGGANQIVKNRVRKNSSQESAKGGAAFLAVCRGKVSEGIDFSDDNARVVVIVGIPFPNVNDVQVKLKKKYNDSYKSSKNLLSGSEWYCHQAFRALNQAAGRCIRHKLDYGGIILIDERYQEERNLLHISKWLKNSIKHYGSFQETMDKLEKFFQKAEEHIKIKRQEMSTKDKLDDDLLISCVDKIKLPCPESNFSKNPVLQSNQKGKLDDDLLTSHGDTRKRPWPEPYLSKHSVLQSNQKVKNKRGSLKVSNIDVAGLDRKNYGMPYTSSGACQVSSKSSGLTKKESSPVLGSLSTACQLPPSHNIQSNLEDEADIWGNHEVDINVTDLNNCDSMPGHVKLTIFTPPEGRSQEPTLVEGTSTEDPFVSPSDCSEVNTSAAMYNDEDQVVGMSASLSTANRNQSCVSTSAGTPETTANRGYHLDHESSVNRSVNSQYQKRRRFNIPMSCCTGTEHSNPSLNPSNPSLDPSNPASTSLCNTDCAVSMVTGDLKRTDGQCCKSMEMSRCENFKLERSHKPVEFASNKSAGTKLDIRCTRCKTPLGLQKDGFLVSSSLASPSKFYLTYLFRNGLSTVGFTENLMASPPAVLNVIVCDASSLNQELLSKFCTEGSAHQSGVWSEKDGCVYKAVTCPFCCRENSFAMVLGAQVLAADASNLESLNKVLLFADRLDFKKERSNEQVARYQTVASNPMGTDASNPMSPPPVINLESFAYKPVKKEPVPLSTRRSKLRLPSTNTSTKPHLVHNVEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS94</accession>
    <name>A0A8I6XS94_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS94"/>
    <dbReference type="SMR" id="A0A8I6XS94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0396420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0396420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0396420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0478440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04714">
        <property type="entry name" value="BAH_BAHCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000561">
        <property type="entry name" value="Bromo-adjacent homology (BAH) domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="86"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="203"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="281" mass="31519" checksum="61829EEFEC05F9E3" modified="2022-05-25" version="1">MTPLGFKHPFHIFTAPPRNPRTTPPSPASSSLVGTEPWQPHPSPPFPSSPPLSFLGFPPDPTPTSMAKTKQGKRDIDSYTIRGTTKVVRVGDCVLMRPSDTDNAPYVARVESLESDGRGSVRVRVRWYYRPEESKGGRRQFHGAKELFLSDHFDTQSAHTIEGKCIVHSFKNYTKLDNVGPEDFFCRFEYKAATGAFTPDRVAVYCKCEMPYNPDDLMVQCEGCKDWFHPTCMGMTIEQAKKLDTFLCADCAKENGAKRPSNSYPSSPSSDSKVEPKRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSP9</accession>
    <name>A0A8I6XSP9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123444992</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044977837.1">
        <property type="nucleotide sequence ID" value="XM_045121902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSP9"/>
    <dbReference type="SMR" id="A0A8I6XSP9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304020"/>
    </dbReference>
    <dbReference type="GeneID" id="123444992"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0253740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0253740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0304020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444992"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000005">
        <property type="entry name" value="Outer membrane OMP85 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.310:FF:000016">
        <property type="entry name" value="Outer membrane OMP85 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF50">
        <property type="entry name" value="BACTERIAL SURFACE ANTIGEN (D15) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0934">Plastid</keyword>
    <keyword evidence="1" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="5">
        <location>
            <begin position="187"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="531" mass="58657" checksum="D3348EC8F5FD40D6" modified="2022-05-25" version="1">MAIAADSTAEQAPNPAPHEPSYEPRKDPYQPDRGEDNEVDFDEEEEDDAEELDGSAARAAYKEKVDALFRRLSTEPVGIRVHNVTVRGNFKTRGSLIEAEVADLLRSAGTVQGLLRASSHATALLRRLDVFDSVSITLDAGPPEFPGTANVIIQVVEAARPYNRGIFFFPKKEAISWPVEGWFMLKNLFGYGDMWDVSGEYGWDQSSRIEIGVYLPRLKSIPTPLTARASILSEDWLKFSSYKERRLGLKFGLLSTWRHNLSYDLAWRALTDPSQMASESIRRQLGHNLLSALSYAYKIDKRDSEFRPTKGYAFVSTSQVGGLWKNGLRFFRQEFDVRGAVPFGFCNAALNVGISAGVILPLSRGFMELSSPVPARFYLGGLSSPVCSLGGLSSLLGFKTRGVGPSEPRRSVPGKSVTVDAAASTGRDYLGGDLAISAFADLSFDLPLKLLRDNGIHGHAFLCAGNLAKLSEGEFKNFSFPEFRRTFRSSAGVGIVFPTSLFRMELNYCHILKKHEHDHGKSGIQFSFSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XT91</accession>
    <name>A0A8I6XT91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XT91"/>
    <dbReference type="SMR" id="A0A8I6XT91"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF146">
        <property type="entry name" value="TF-B3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="49"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="157" mass="17540" checksum="42C09FC5777845DA" modified="2022-05-25" version="1">MDKDKEVVPSLGKGREVAPPLVEEPVQDHLSPKRRNCGHYHQESGPNHFCKVILAPKLESLPLPLNFTKHFPAIPAEFKLKTNTICAWRVTVRLMNGRVTLDQGCDTFVAVHHIMIGYMLTFKLLTPDTMKVIVFDGEGVEVVTKCKEHDNASVAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTL4</accession>
    <name>A0A8I6XTL4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973169.1">
        <property type="nucleotide sequence ID" value="XM_045117234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTL4"/>
    <dbReference type="SMR" id="A0A8I6XTL4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309280"/>
    </dbReference>
    <dbReference type="GeneID" id="123440676"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0258200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0258200.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0258200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0309280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440676"/>
    <dbReference type="OMA" id="RECCEAV"/>
    <dbReference type="OrthoDB" id="643149at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04660">
        <property type="entry name" value="nsLTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044741">
        <property type="entry name" value="NsLTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF45">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR_PLANT LIPID TRANSFER PROTEIN_SEED STORAGE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035154697" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309280.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="10379" checksum="92FC0D8D2C2FEEF0" modified="2022-05-25" version="1">MAAGRKVMSNAGVAAALVVLLVVAAASSVAGVCKVDTGSMVRNCRTYCTVGSTEASPSSACCGAVRGGDFHCLCQYRSVLPNDIDANRAMQIPGKCGYGAANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVL0</accession>
    <name>A0A8I6XVL0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123441219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Poa p IX/Phl p VI allergen family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973647.1">
        <property type="nucleotide sequence ID" value="XM_045117712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVL0"/>
    <dbReference type="SMR" id="A0A8I6XVL0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330420"/>
    </dbReference>
    <dbReference type="GeneID" id="123441219"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0330420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441219"/>
    <dbReference type="OrthoDB" id="678986at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.320">
        <property type="entry name" value="Group V grass pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035506">
        <property type="entry name" value="Pollen_allergen/Os"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288337" evidence="2">
        <location>
            <begin position="30"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="128" mass="13439" checksum="DEA91A70693EA518" modified="2022-05-25" version="1">MAPLQNIAVALTLLIVMVEIASLPMLASAAIVKSEEAALAELTPIIKTALDGVLAAAPPSERIKVAGAVAKQELLAMDTMKKAKGDKAKFDTHLLAYKIAAKIVTAAAPAEKFKKMEDSFTEASRPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XW70</accession>
    <name>A0A8I6XW70_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XW70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0533750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="137" mass="16394" checksum="68E17B83853128E8" modified="2022-05-25" version="1">MNFQDVQTLQQALDLAPPPRLNSAQDRAEHNALQRRLLVAQEDERVMAEWRRRHPEDVAYEQQYWASRREEDTRRRREERLDRRRRKALACAQADLVNAGGRSFFTEEDERWLDCWLSTSDDTNDDDDGADDWSDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWE8</accession>
    <name>A0A8I6XWE8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123447147</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UPL family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044979659.1">
        <property type="nucleotide sequence ID" value="XM_045123724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWE8"/>
    <dbReference type="SMR" id="A0A8I6XWE8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334560"/>
    </dbReference>
    <dbReference type="GeneID" id="123447147"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447147"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="10">
        <location>
            <begin position="829"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="548"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1173" mass="132119" checksum="C70F314CBACBFD00" modified="2022-05-25" version="1">MQKQPPSTSSPPAMSVPPSGHRQVSLRGSSAREITRDALLQKVSEERQLRSHLRRAAAAALTIQRVWRKYHLIRKVTEQLHEEWEVLINQPDINLTNQWISSKMLRPFLFFITQPSNWYKGQQTKTVKSISRCFKIILYSINSTDQSKNFCSFAVGFPEERSIWLYQAKKLISLCSCILARCDHCCKDVNMVEITTLTMRLAISLTDCKTWKNLTSENTRAADASVEALIEFIGTRQSGTYSCVRRYIKCFDPHVTPGKIDSAIAPDDQLLVTASAVTLALRPFNSTRADMGVDLTGSAKEYFTLILSIPYICKRLPPLLLPALKHISVLQPSLSILLISKDKIFEEISKLELAEVSYVDNSTIPYCGWALGNLITLATEHDDLSNSGCFIQGLDCCLYVHAINCISQNLLKCFEESKGMLHCIDDRATNNTSITEEVDTNDSFRIRTLFMDLLKPIYQQWHLRKLLILAKEDVPYERETNHDLDQRQVKCRSLKLTDIICFYYYMLRIFSSLNPSIGPLPILNMLSFTPGFLVDLWGTLEISIFGQTGHKSQEPEHEKQLAGSSSGEQISSTRQRRNAKDAPKKWANVLHKITGKSTDADDTNLSDSLTSESSNDDALILWDIETMRQGSEGITKDVMHMLHLFCAIYGHLLLVLDDIEFYEKQIPFTLEQQRKIASALNTFVYNSFLQNSGSGNKPLIDVTVRCLNLLYERDSRHRFCPNSLWLSPARTGRIPIAAAARAHEAAFASLVGTTSGIPTRSSVLTTVPHVYPFEERVQMFREFIELDKASRRANGEVSGPGPGSIEIVIRRGHIVEDGYRQLNCLRSKLKSCIHVSFVSECGLPEAGLDYGGLSKEFLTDLSKAAFSPEYGLFTQTSTSDSSIIPSSSAKLLDNGIDMIEFLGRVVGKALYEGILLDYCFSQVFVQKLLGRWSFLDELSTLDSELYRSLMQLKYYDGDVEELCLDFTLTEELGGKRIVHELRPGGKNISVTNENKLHYVHAMADYKLNHQILPFSNAFYKGLSDLISPSWLSLFNANEFNQLLSGGSQDFDVDDLRNNTKYTGGYTESSRTVKLFWEVIKGFKPTERCLLLKFVTSCSRAPLLGFKYLQPGFTIHKVPCDVTLWASIGGQDVDRLPSASTCYNTLKLPTYKRSSTLRSKLVYAISSNTGFELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XY22</accession>
    <name>A0A8I6XY22_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF642 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123452880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044985543.1">
        <property type="nucleotide sequence ID" value="XM_045129608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XY22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522760"/>
    </dbReference>
    <dbReference type="GeneID" id="123452880"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0434600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0434600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0434600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0522760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452880"/>
    <dbReference type="OrthoDB" id="1851446at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000031">
        <property type="entry name" value="DUF642 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006946">
        <property type="entry name" value="DGR2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052437">
        <property type="entry name" value="Pectin_Meth_Modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265:SF2">
        <property type="entry name" value="F17A17.37 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265">
        <property type="entry name" value="OS02G0527500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04862">
        <property type="entry name" value="DUF642"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035190947" description="DUF642 domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="8">
        <location>
            <begin position="53"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="8">
        <location>
            <begin position="222"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="393" mass="41330" checksum="006227C813D6567F" modified="2022-05-25" version="1">MELPWGALLLALLCVSASSAVATLAVTAPPPAPARAPAPVTAPAPPQAQDAEGLLMNGNFETAPRKVNKTLIVGRHSLPGWTLRGHVEYVSAGPQPGGMFFAVPHGVHALRLGSHASASQNVSVRPGSLYALTFAATRTCAQDEALRIAVSPSLSAPADVAVRTLYSADTADTWAWGFRASSPVAQVTFSNPGLQEDAACGPLIDAVAIKELPTPYPTKDNLIKNDGFEIGPQVFKNSSVGVLLPPKQKDVTSPLPGWIIESLKAVRYIDAAHFSVPAGQYAVELVAGRESAIAQVIRTVPNRAYNLSYVVGDAKNGCHGSMLVEAFAANVTQKVPFESRGKGGFKASSLRFVAAGARTRVTFYSSYYHTKVTDGVSLCGPVLDQVKIVPLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYY0</accession>
    <name>A0A8I6XYY0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7906 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123453097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044985790.1">
        <property type="nucleotide sequence ID" value="XM_045129855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYY0"/>
    <dbReference type="SMR" id="A0A8I6XYY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527440"/>
    </dbReference>
    <dbReference type="GeneID" id="123453097"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0438690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0438690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0438690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0527440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123453097"/>
    <dbReference type="OrthoDB" id="16573at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057228">
        <property type="entry name" value="DUF7906"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31515:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31515">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25483">
        <property type="entry name" value="DUF7906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035218687" description="DUF7906 domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="925"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7906" evidence="4">
        <location>
            <begin position="387"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="954" mass="108130" checksum="08946FC110D31683" modified="2022-05-25" version="1">MRSPFRLPAAPFVSALVLLMLALVPSPEAAETTTPGTRTRKLGPGGSSSVFSLFNLKPQSKFWTESVMRTEFDDLVGSKSRDSINVGLLNFTRAGNVANYMNLAEVDSIYFSIPVNFIFMGFDGKGGHEFKLGPEELERWFTKIDHIFEHTRIPPVGEVLSPFYKTTVKRLKKYDLPLVSHINHNFSVHAIHMGEDIMSVFEHAVKVLSRREDLTDPSESGAIWQVDSDQMEHVFSTLVDHLQIQEAYNIFILNPKPIEKGTQYGYRKGFSESEINLLRENKTLQARILQSKRDPKLYFDIEKGANKRPLYENHPLSSFSWTTTDNVDMGDWSKQCKEALSGFELLKAGKSKEDIIYDKAVQILHGAKDELHDVLESALISSDLKGLHAECLTDIWVGRNRFAFVDLTAGPFSWGPAVGGDGVRTELTLPNVAKTVGAVAEITEEEAEENLQDTIRERFSSVGEDYHAVDILLAEIDVYELFAFKHCMKRKIQLALCKELDQRMHELKNELEGYHSGDSEEINKKKALDALKRMEKWNLFKDTSEEHHSYTVARDSFLAHLGSTLWGSMRHVIAPSVSHRAYHYYEKLSFQLYFVTQEKVRNIKQLPINVKSIRDGLSSLLLPSQKSMFTQHMLSLSEEPALMMAFSMARRAAAVPLLLVNGTYRSTVRTYLDSAILQHQLQKLSEQTALKGEHTNHRSTLEVPVFWFIHSESLLLDKHYQAKALPNMVVVVQSDVDSWESHIQCNGRSILWDLRKPVKAAIAASAEYVSGLLPSHLAYSSAHETASEDWTWSVGCNPLSISSKGWRLSEFQQDVIARNYIITGVEESIRVVNSAIQRLITERTSEQCFKIFKTKESVMVEKYNSVVNMWRRVAFMSKGLRYGDAVKLMSSLEDASNGFSHAVNSTISSLHPAKCARQRKIDVQLDMTTIPAFIVVFGLLWFLLRPRRPKPKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZY4</accession>
    <name>A0A8I6XZY4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZY4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0407710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0407710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0407710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0491960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="270" mass="29782" checksum="2D32E1C8BABA600A" modified="2022-05-25" version="1">MLGLAVRCGGGGCGCGGAGAYLTAQRTTAARSGAGRSGVLAAGTGARGLSLRVSFSPNSHPHRVGTSCPWPVRCSPGPSPDLRVSKKKRDEAWDALKTEIGRSFRPHLRNLGEIFSLRSPYDLEDYQIGMLFGAFLGCVGCYQLWKTAPSIFVDAFLAFIFYKLSVVSSELHRNDKSNSLITRLKFGTILIMVWKDIKKTYVLLDVVRMPVFLLYICAFLFDVAGVKNYGRRGLVYFVNLMKTRGGIQEICRIMWLPGYISPYDDGLNWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0V8</accession>
    <name>A0A8I6Y0V8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123445195</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978086.1">
        <property type="nucleotide sequence ID" value="XM_045122151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0V8"/>
    <dbReference type="SMR" id="A0A8I6Y0V8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309460"/>
    </dbReference>
    <dbReference type="GeneID" id="123445195"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0258360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0258360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0258360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0309460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445195"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF18">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="151"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="204"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="55"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="447"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="55224" checksum="8BFBFEBC9805D934" modified="2022-05-25" version="1">MSLPGKKAAAGAGGIRRWLSTAVLSALALVLILVVISLSVGSPLPGASLPEYPAVRASDPKGNGTADDGSKDDIASGVPLPGKELQGGQEPLAEHSAQNGTLNSSEGSQNTREVDDTVPDPVSVDSKIQDPVATDDTTPKLNDSQRADQGACDLYHGEWIFDSSGPLYTNNSCPIITQMQNCQGNGRPDKDYESWRWKPEQCTLPHFDGSKFLELMRGKTLAFVGDSVARNQMESLLCILWQVEAPVNRGSRRMSKWIFRSTSTTIIRIWSSWLVHKSIEAVGFAPNGLDKVFLDVADETFMQSIASFDVLVLSSGHWFAKRSAYILNGDVVGGQLWWPRGAGKMQINNVDAFGVSVETCMTAVATHPNFTGIAVLRTYSPDHYEGGAWNTGGSCTGKVKPLDVVVRNGYTDTMYEKQIAGFRKAVQNSGKHSSKLKLMDITEPFALRADGHPGPYRSPDPNKKTQRGPDGKPPPQDCLHWCMPGPVDTWNEMLFETIRRELEVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0Y3</accession>
    <name>A0A8I6Y0Y3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123446750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0Y3"/>
    <dbReference type="SMR" id="A0A8I6Y0Y3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0394410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0394410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0394410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0328180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0328180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0328180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0394410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0394410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0394410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052610">
        <property type="entry name" value="bHLH_transcription_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45959:SF59">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45959">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="135"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0394410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="314" mass="34085" checksum="E5E34DB0E356E59F" modified="2022-05-25" version="1">MEQPNQWLTQTEQEELAYMYQQEGVPVPGMDQQFAEPPLPGHQEQYYTSPMATPSFHTSRSSNFASFGGSSSLPNLPFGSATVKNELGQPPSSNFLSFGAGQAGTLNFSGGAWQHDGVEGTMQLQAPERRSRAPANAQEHVIAERKRREKLQQQFVSLATIVPGLKKTDKISLLGSTIEYVKELEDKVRALEEQGSRRSSESTTVFESKCRISAADNDAAGPSGSGDAEESSPAVEASIRGHTALLKICCKERRGVLVMVLSELENQGLSIINTNVVPFTDSCLNITITAKIEEGFSSAAELVRNLTVALRSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y466</accession>
    <name>A0A8I6Y466_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodopsin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y466"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0437090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31248">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G04290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31248:SF31">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="18154" checksum="37F426BB7B0300B9" modified="2022-05-25" version="1">MDGGKDKGLHGHGGYPGGYPAPPGYPGYPVPVSTYPPAPGYAAPPGQYAPPPGAYPQPGGYQPPHAAYPPSGYPAIAGYPQYPTTHPPAGYPAHGPPMAVPPYGHAPMYGGGGHGVAGGVIAGGAAAAGAAYGAHKVSHGGAYGMYGHGHGHHHGKFKHGKFKHGKFGKHKKMFGRKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4M3</accession>
    <name>A0A8I6Y4M3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123399422</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in ammonium transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949768.1">
        <property type="nucleotide sequence ID" value="XM_045093833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4M3"/>
    <dbReference type="SMR" id="A0A8I6Y4M3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530810"/>
    </dbReference>
    <dbReference type="GeneID" id="123399422"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399422"/>
    <dbReference type="OMA" id="VPTDQNW"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000005">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF2">
        <property type="entry name" value="AMMONIUM TRANSPORTER 3 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="25"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="470" mass="50055" checksum="7C0823D4C9C8C0F0" modified="2022-05-25" version="1">MSVPVAYQGNTSAAVADWLNKGDNAWQLTASTLVGLMSVPGMVVLYGGVVKKKWAVNSAFMALYAFAAVWICWVVWAYNMSFGEELLPFWGKAGPALDQAFLVGRASLPATAHYHADGTLETAMVEPFFPMATVVYFQCVFAAITLILVAGSLLGRMSFLAWMLFVPLWLTFSYTVGAFSVWGGGFLFQWGVIDYCGGYVIHIPAGVAGFTAAYWVGPRTKKDRENFPPNNILFALTGAGLLWMGWAGFNGGGPYAANVDSSMAILNTNICTAASLIVWTCLDAVFFKKPSVVGAVQAVITGLVCITPGAGVVQGWAALVMGVLAGSIPWYTMMVLHKRSKLLQRVDDTLGVIHTHGVAGLLGGILTGLFAEPNLCNLFLPVTNSRGAFYGGVGGAQLGKQIVGALFVIGWNVVVTSIICVVIRLVVPLRMPEEKLAVGDDAVHGEEAYALWGDGELYDDTKHSAAVAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBU7</accession>
    <name>A0A8I6YBU7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBU7"/>
    <dbReference type="SMR" id="A0A8I6YBU7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0677640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF92">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="3">
        <location>
            <begin position="28"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="654"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="940" mass="108525" checksum="E24E4791598141A0" modified="2022-05-25" version="1">MTTYVTNLLCCHICTDSASGLWVTMREYYCYKFHTRPSIFNPILHGGWIFQQFVVDTYIKIESSRLDFIWHNQKKIRADLYQGLLDSIQAGEQNGDAVGKTRILASSFIGGPRDKLRRYLDAMALVRKYGKPDVFLTMTCNPNWEEITRELETGQTPQDRPDIVVRVFRAKLQEMKKQLFEKAILGKVQAYTYVVEFQKRGLPHAHFLLIMTGKYKYTCPEQYDRIIYAELPNKHKYPDLYRMVIKHMMHGPCGALNRLCPCTKNRPSCKNNYPRAFNETTIQGKDSYPIYRRRDDGRTETARNCKLDNRWVVPYNPYLLKMFNCHINVEIFSSIKAVKYLFKYIYKGHDRASVSITDKADDVEIDEIKQYRDARWVTPPEALWRIYGFELSKMHPPVLKLHLHLPNMHMVSYHGMEKIQNVIDREGTERSMLTAYFEANSLHEKARGILCREFPEHYTWQSQGKFWQQRKRAAVFQVGRIVSAHPAEGERYYLRVLLNHVTGATSYEDLRTVDGQLMPTFREAAEKRGLIEADSTLDDCMTEAELFQMPSSLRRLFATILVFCEPSDVCSLWNKHLEAMSEDYTRNCKFKHTVKQMVLKNIRDMLQSMGKDIESFPLPKIDQQHETTDDVPREITEESSIEVDHEDISLYENLNNEQREAYDKILAAIECQRGGIFFVDGPGGTRKTFLYRALLATIRGQGKIVVATATSGVAASIMPGGRTAHSRFKIPLKIDDGANCGFTKQSGTAKLLKATSLIIWDEASMTKRQTVEALDKSMRDIMDKQELPFGGKTVVFGGDFRRVLPVVRKRTRAQIVDASLRRSQLWNSMCQLKLVRNMRAQNNKWFADYLLCIGNGTEETNEDGEILIPTSICVPNNTDDNSLDGLIDSVYQMDNASLKDPKYITSRAILSTRNDSVDRINLKMIDRFQGRRWYTTASIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBV0</accession>
    <name>A0A8I6YBV0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123407889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957060.1">
        <property type="nucleotide sequence ID" value="XM_045101125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBV0"/>
    <dbReference type="SMR" id="A0A8I6YBV0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703000"/>
    </dbReference>
    <dbReference type="GeneID" id="123407889"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0583210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0583210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0583210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0703000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407889"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF38">
        <property type="entry name" value="UDP-ARABINOSE 4-EPIMERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="6">
        <location>
            <begin position="66"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="405" mass="44712" checksum="C39061DEBD94F144" modified="2022-05-25" version="1">MIPVNRRGTQPRAGMEYFDARRKPHNIGKVIVALLFLALCIFVLKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSHASLRLLKDNYRVTIVDNLSRGNKGAVKVLQELFPEPGRLQFIYADLGDQKSVNKIFSENAFDAVMHFAAVAYVGESTMEPLRYYHNITSNTLLILEAMASHGVKTLIYSSTCATYGEPEKMPIIETTPQLPINPYGKAKKMAEDIILDFSKRTDMSVMILRYFNVIGSDPEGRLGEAPRPELREHGRISGACFDAALGVISGLKVKGIDYPTADGTCIRDYIDVTDLVDAHVKALNKAKPSKVGIYNVGTGRGRSVKEFVDACKQATGVDIKIEYLSRRPGDYAEVFSDPTKINNELNWTAQHTDLKESLSVAWRWQKSHPRGYGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCF1</accession>
    <name>A0A8I6YCF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123401302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951011.1">
        <property type="nucleotide sequence ID" value="XM_045095076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0574580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0574580"/>
    </dbReference>
    <dbReference type="GeneID" id="123401302"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0476360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0476360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0476360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0574580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0574580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0574580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401302"/>
    <dbReference type="OrthoDB" id="686619at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47746:SF88">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE)-RELATED FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47746">
        <property type="entry name" value="ZF-RVT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="246" mass="27298" checksum="3EC06A10BC3E533C" modified="2022-05-25" version="1">MQCRGMNHIPFLDPDVPVYIRSKQDWLEAEKAMLAKKEAKIRFIYECPGDSPIQAKLDGARMPAGLAKGGDGAGLAKGGVDAGLAKRGEGAGLAKRNDHQGSVKYIVPARRGLTADNLERRGWPNQGTCALCSLAPESCGHLFVHCSFTAEVWQRFRTWVGVPFPTPAADDKGTEDWWLRARNAAPERFKRDFDAVVILVYWRLWKERNARIFDGIASSPDRVVEAIIEDLRSWREAGCVATTIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YE14</accession>
    <name>A0A8I6YE14_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YE14"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0701290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26452" checksum="92F9E658D4847DE9" modified="2022-05-25" version="1">MLVRVMLVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPNYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSDFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFJ3</accession>
    <name>A0A8I6YFJ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFJ3"/>
    <dbReference type="SMR" id="A0A8I6YFJ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0512210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0512210.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0512210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0617550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617550.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="124" mass="13951" checksum="3D3BB3A56A745C05" modified="2022-05-25" version="1">MEKMKDAPVPEGEEPKSDAEIVEEVLKTEVNQRTFLRNVGLNSSSNNSGKGTTIVAAHVRDLQQKLERSELQAEVMQEEMAAIKLKAEEYEAARDKELELLRKKSQEQEEKLAHLMALFGAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGH4</accession>
    <name>A0A8I6YGH4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 20">Peroxidase</fullName>
            <ecNumber evidence="3 20">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC123402992</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17 20">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 20">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="17 20">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 20"/>
    <dbReference type="RefSeq" id="XP_044952902.1">
        <property type="nucleotide sequence ID" value="XM_045096967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGH4"/>
    <dbReference type="SMR" id="A0A8I6YGH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547280"/>
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        <property type="component" value="Chromosome 6H"/>
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        <property type="entry name" value="Peroxidases_AS"/>
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    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
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    <dbReference type="PANTHER" id="PTHR31388:SF228">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
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        <property type="entry name" value="PLPEROXIDASE"/>
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    <dbReference type="SUPFAM" id="SSF48113">
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 20" id="KW-0349">Heme</keyword>
    <keyword evidence="14 20" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 17 20" id="KW-0408">Iron</keyword>
    <keyword evidence="6 17 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 20" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035337273" description="Peroxidase" evidence="20">
        <location>
            <begin position="25"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="21">
        <location>
            <begin position="25"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="66"/>
        </location>
    </feature>
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        <location>
            <position position="67"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="70"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
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        <location>
            <position position="169"/>
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        <ligand>
            <name>substrate</name>
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    <feature type="binding site" description="axial binding residue" evidence="17">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
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            <position position="62"/>
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            <begin position="35"/>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="333" mass="35662" checksum="C3375ED716260CE2" modified="2022-05-25" version="1">MAASFTSRCAMLALLALFLSGADGQLSPTFYASTCPNLATIVRSAMNTAVANERRMGASLLRLHFHDCFVEGCDGSVLLDNVPATPTTPAFFGEKNAFGNINSVRGFEVIDTIKSNVEQACPGVVSCADILTLAARDGTVLLGGPTWEVNLGRRDSMTASMDLANRDLPPPFANLVNPDGSGLIARFANKGLNATMMTVLSGAHSIGFAQCKNYRDRLYNEPNIDFQFAQQLQANCPATGPAGDTFLAPFDVQTANAFDNKYYDNLVSRRGLIHSDQVLYSPGGTLASVVNQYRSNNALFLSDFVVAMKKMGDLSPTAGSASQIRTNCRFVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHG3</accession>
    <name>A0A8I6YHG3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHG3"/>
    <dbReference type="SMR" id="A0A8I6YHG3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0598760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0598760.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0598760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0721960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF1">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00108">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50927">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035221592" description="Receptor-like serine/threonine-protein kinase" evidence="19">
        <location>
            <begin position="23"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="21">
        <location>
            <begin position="31"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="22">
        <location>
            <begin position="338"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="527"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="851" mass="93365" checksum="163A60C2896A9EF4" modified="2022-05-25" version="1">MRVLAILLVITTAATLPHVAPAASDDQRPTSILWRRGSIAVGDDALVSPSGDFSCGFHRVATNAYVFAVWFTVSADPPTLAWAANRDAPVNGMGSRAELLVDGTLVLRDFDGMAVWSTNTSGTGADRAQLLDTGNLVVSDAAGRTLWQSFDWPTDTLLPGQLITRRARLVSAKARGSTSSGYYSFYFDNFNILNLVYDGPEINMNYWPNPFKTWVENKRTAFNSSRLGSLDDRGRFSASDNLRFAVSDMGTTGHVMRRLTLDYDGNLRAYSLDAAGGGAWRITWAALGRQCSVHGICGRYGVCAYGPAGPACACPEGFVPSDPGDWSKGCRRLFDIQCGEDVFFAELANVDYWGFDKKFRQKATIDECQQLCIDDCRCEAFAYKKGGGGLCYTKVSLWNGRSSDPIQFTYFKVPTRVEKKLNLSSMLRLRFDGHDCSTATTQERNASVGYLPSRHLKNFSGSKIKFVYLYSFIGGLFVVEAVVIIAGYLLVFRADQAAVQHVHDDGYTLAMSHFRKFAYDELSIATCDFGEELGRGASGAAYKGVLDDGRDVAVTRLMEATTPQAEEVFRSELSVIGRINHMNLVRIWGFCSERSHRLLVSEYVENGSLAKALFGGEPALGWPSRYKIAVGVAKGLAYLHHECLEWILHCDIKPENVLLDADLEPKITGFGLVKLLSREEDACGSKAPSRVQGTRGYVAPEWALSLPITGKADVYSFGVVLLELLRGQRVSEWAVVDGVVAGDEEVARVDLQQIVAWFQDQTLECQGDQSAPAPWLEEFVDARLLGDFDRLQAAAMLEVAVSCVEGDPSRRPSMNAVAQKLLSTQDAVGSASLRRVCPVPSHGRINSLRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHJ0</accession>
    <name>A0A8I6YHJ0_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6YHJ0"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0487620"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0403980.1.CDS.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF10">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0487620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="17145" checksum="4E433BEF3CD214C3" modified="2022-05-25" version="1">MQFCIGQLWSMHDKENEDRIKENLKLGEENFKIIEEKRKMEEELRFFKLDFEKMVAHKEEAINQLGSARLAINDSKEEIEKKILADHACTNLHQVLRAKAEKKRNQLVLERDQTKEEKKKLEYIISDMMKQNHGYKDKVKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHV1</accession>
    <name>A0A8I6YHV1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHV1"/>
    <dbReference type="SMR" id="A0A8I6YHV1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0602430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0602430.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0602430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000128">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1392">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="50843" checksum="1742BA96EB3E0106" modified="2022-05-25" version="1">MDAEVHVLVFPWPRQGHINPMLQFATALVDAGVQVTFLHTEHNLRRLAQASPPHLRLLSIPDGLPDDHPRGFLDFMEAMCTTGSTAYHALLLSLVSASDAPVTCVVADGTMPFAIEIPQELGIPALSFVTHSACSYLALLSMPKLVELGETPFTADDLVSSVPGMEGFLRRRDLPRGLYCTDQGDGDPLVLKFAEVIGRTSKACALIFNTAASMERPVLAHLASGTSDVFAVGPLHARSSSAASASLWREDDGCMAWLDGHEDRSVVYVSMGSLAVITHEQFTEFLSGLAATGYAFLWVLRPGMVQLASSALLREAVGAAEGGKGRVVEWAPQRDVLRHRAVGCFLTHAGWNSTLECAVEGMPMVCWPFFVDQQTNSRFVGAVWKTGLDMKDVCERGVVERTVREVMASDEIKAAAQAMAQQLKHDVAEAGSSSSELERLVRFIRELSIRPPSAELMETRAQAAVTVSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJ15</accession>
    <name>A0A8I6YJ15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123412805</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961683.1">
        <property type="nucleotide sequence ID" value="XM_045105748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJ15"/>
    <dbReference type="SMR" id="A0A8I6YJ15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0735770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0735770"/>
    </dbReference>
    <dbReference type="GeneID" id="123412805"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0610310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0610310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0610310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0735770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0735770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0735770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412805"/>
    <dbReference type="OrthoDB" id="744205at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF177">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="11"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="300" mass="33479" checksum="8D35CEDB29FF378A" modified="2022-05-25" version="1">MSSLSMVEARLPPGFRFHPRDDELVLDYLSRKLGGGAGPAVASIYGCPAMVDVDLNKTEPWDLPEIACIGGKEWYFYSLRDKKYATGQRTNRATESGYWKATGKDRLISRKGLLVGMRKTLVFYEGRAPKGKKTEWVMHEFRKEGQGDHMKLPLKEDWVLCRVFYKTRTTIAKPPTGSSYKIDSAVATSLPPLIDNYIAFDHPGMLQNLEGYEQVPCFSNNPSSHPSSSTSMSIPGMAPMAADQEQQHMGKAIKDVLSQLSRFEQGNVKREAPTQGGVFAQDGFEYLAESGFSQMWNTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YL71</accession>
    <name>A0A8I6YL71_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Galectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123452823</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985481.1">
        <property type="nucleotide sequence ID" value="XM_045129546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YL71"/>
    <dbReference type="SMR" id="A0A8I6YL71"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521630"/>
    </dbReference>
    <dbReference type="GeneID" id="123452823"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0521630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452823"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990714">
        <property type="term" value="F:hydroxyproline O-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000005">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF241">
        <property type="entry name" value="GALECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galectin" evidence="14">
        <location>
            <begin position="149"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="632" mass="69954" checksum="A3E7FA71F02671C5" modified="2022-05-25" version="1">MAMPPKRACRLALMLAAAGYLCFLLFLELPTVSIAPVATADRPRHRELEARSSSTVAPLRPHTRTFPTPDHPPRRSRLAVSSIRVRTSTSTSSIDAAASAAFAAAAPLLPDLLLSPSPSSSSPSPSPSAPASCPAAVSVPGDRLAGTPVAVELPCGMAVGSRVTVVARPRRRMDGASSSASSQFMVELLGTKAVQGEEPPRILHFNPRLAGDFSGRPVIELNTCYRMQWALPQRCDGSASRPDHDTVDGEIKCEKWIRDDGAKSEESTTNMKWLLNSLIGRPDPEKVSVDQDQAYPFAEGKLFALTITAGLEGYHVNVDGRHVASFPYRTGYNLEDATGLSLNGDLDIESVSANHLPKSHPSFDPQRYLEMSEQWKASPLPTEPVELFIGILSSANHFAERMAVRKSWMIATRRSSNSVARFFVALNGKKEVNEELKKEAEFFGDIVLVPFMDSYDLVVLKTIAIAEYGVRVVQAKYVMKCDDDTFVRIDAVLDQVKKVKNGASMYVGNINYYHRPLRSGKWAVTYEEWEEEVYPPYANGPGYVISSDIAEYIVSEFDNQKLRLFKMEDVSMGMWVQKFSKTRQPVEYSHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YN41</accession>
    <name>A0A8I6YN41_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Defensin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEFL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YN41"/>
    <dbReference type="SMR" id="A0A8I6YN41"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0738930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0738930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0738930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0612900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0612900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0612900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0738930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0738930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0738930"/>
    </dbReference>
    <dbReference type="OMA" id="AKCTTEY"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010851">
        <property type="entry name" value="DEFL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33830:SF3">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33830">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 184-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="3" id="KW-0295">Fungicide</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035295174" description="Defensin-like protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0738930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8426" checksum="84DDA811EE56D886" modified="2022-05-25" version="1">MKPCQVTLLLAMVILASSPAMATKCPPEYTCTKYINKGMCDAAKCMADCVSAYKGAGEGQCFTQGCRCSYCCKPHQGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YP21</accession>
    <name>A0A8I6YP21_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123411479</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960368.1">
        <property type="nucleotide sequence ID" value="XM_045104433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YP21"/>
    <dbReference type="SMR" id="A0A8I6YP21"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747040"/>
    </dbReference>
    <dbReference type="GeneID" id="123411479"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0619680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0619680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0619680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411479"/>
    <dbReference type="OrthoDB" id="10253031at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="115"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="17055" checksum="8BE17D1FF703837B" modified="2022-05-25" version="1">MAGWKGQRKKAVTRSVKAGLQFPVGRIGRYLKQGRYAQRIGSGAPVYLAAVLEYLAAEVLELAGNAAKDNKKTRIIPRHLLLAIRNDEEIGKLLTGVTIAHGGVLPNINPVLLPKKTAEKANKESKSTKKKKKAAAESPEKAAEDTATATEETAAADEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRJ0</accession>
    <name>A0A8I6YRJ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123403405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953275.1">
        <property type="nucleotide sequence ID" value="XM_045097340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRJ0"/>
    <dbReference type="SMR" id="A0A8I6YRJ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554560"/>
    </dbReference>
    <dbReference type="GeneID" id="123403405"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0460660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0460660.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0460660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0554560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403405"/>
    <dbReference type="OrthoDB" id="551672at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14702">
        <property type="entry name" value="bZIP_plant_GBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000020">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045314">
        <property type="entry name" value="bZIP_plant_GBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45764">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45764:SF76">
        <property type="entry name" value="OS02G0132500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="36"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554560.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="172" mass="18002" checksum="2B7BFED54315DC17" modified="2022-05-25" version="1">MASSSGSGSTGSLSTAAAMAMAAASGTEEDMRALMEQRRAKRMLSNRESARRSRMRKQRHLDDLAAQAAHLRRENAHVAAALGLTARGLLAVDAENAVLRTQAAELAARLQSLNDIIACMSASNNTAAAVALTVAAAATATATDPFGFDGPSLDDYLCRSSPEMFMFPALQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YV15</accession>
    <name>A0A8I6YV15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123412734</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms homooligomers and/or heterooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961606.1">
        <property type="nucleotide sequence ID" value="XM_045105671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YV15"/>
    <dbReference type="SMR" id="A0A8I6YV15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747240"/>
    </dbReference>
    <dbReference type="GeneID" id="123412734"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0619880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0619880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0619880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412734"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF30">
        <property type="entry name" value="SUGAR TRANSPORTER SWEET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="243" mass="26765" checksum="19581FA7995217FE" modified="2022-05-25" version="1">MVSADAARNVVGIIGNVISFGLFLSPVQTFWRIIKAKDVEEFKPDPYLATLLNCMLWVFYGLPIVHPNSILVVTINGIGLVVEGSYLIIFFIYSTNNKRLKMLGVLAAEAVFMVCVVLGVLIGAHTHEKRSMIVGILCVIFGTIMYASPLTIMGKVIKTKSVEYMPFTLSLVNFLNGFCWTAYALIKFDLYVTIPNGLGTVFGLAQLILYFCYYKRTPKEDKSVELPTSNPAGGGNVSVAVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXT8</accession>
    <name>A0A8I6YXT8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXT8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0512200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0512200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0512200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0617540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617540"/>
    </dbReference>
    <dbReference type="OMA" id="RRWRITI"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="44369" checksum="013C6A24AF524257" modified="2022-05-25" version="1">MVEDNEDDRLSMLPDDVLLNIVERLDIADATRTTILSSRWKQIPAMLSKVVIMVDSFERKHTTSKLTSDDIIRFNTTVLDATRSILERRSRNLYTIHLLRMQFYLGDESIFIGQTVANTIATQKVASVQFRIVTKVRKICTSNDLLTYGRQFMSFFDSCPNTFGCLARLWLENLRLGESGFPKIFSICKQLEFLFLFECDMGIKSLLEVEHPQLSELVIDCGRFERVHLKWAPKLTILKFNVFRSRDDPFCLGYVPLLQTVSITNTGLSWHKMLKLSELLGKTAISNLRLNFKSEKIWVKPEGRRQLFPVFHKLRIVNLRNISEECDLTWTMFLLEGAPNLKELHILVRDHLCEMITGERRKMCPFSEQKDKGLHGNRLRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZ27</accession>
    <name>A0A8I6YZ27_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123401674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZ27"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549420.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549420.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0456560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0456560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0456560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0549420.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549420.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040305">
        <property type="entry name" value="At1g75730-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34792">
        <property type="entry name" value="OS02G0121500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34792:SF1">
        <property type="entry name" value="OS02G0121500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="708" mass="76456" checksum="F4994EA4DF3FDD44" modified="2022-05-25" version="1">MRGVRGERERESEGIRRRNFWMVVDMEEEKKKLAAVVGVTRRHVISSGSGSSLSSPPSLPPPAAAADSNNIIESEGGDHVAELNRKKGKKPKPLKWRSTNSDMNNRRDGEAEEGSDGGRDDTVLSSLTRSTTGCGSLIGRKRARTLGKVAEDCDAVDPPVPRKLRSAIIKRVGQTVPSSPRHAKKRRHLSAISAQIFHMDHETRTDDSMPSNSFTEEEEVLADTLLALSQIAPPSEPTADTATERDISNTNVASTSCSEGVITPTGAIKEDDKITLLPTDANKVVPQSGFADQQVEPAATSSVPQLKPALGAPRISINPDLPKDGQIQDLSLGLFANSSSPPKESFNKSAWKKPKAHFDGSLSLTNHTNKEAPHWLMNHNKSGFAVHDRTKYENNSAKEVTPAIQAPLPCTSTGSSKKPSSSILSGCTISGTEITTAWATANNDKLSLPVNGGPAKTWKRSVNHVYVSHLIQNHLDKEKASQNQVIAQERSRSRISSSPSGSTLNKNGMHFDARIPVQPSVGVCDMAPGRQAMVSNDFMNMPTSAAFSGPQYVQYIHPQMITAHRGPAPYPSYSHLPCSSRGNVAPAMSIQQQMQQYMCSPGYAPHPGAPAGQGAVKLQQFAPTPQQQQQQQMWQFHFSQYQPRQAAEGAAAAWQNGRLRDVPSSGSMRPMQALHAPPAMAPPQMELLCGPYQGGGGGARRPPQLRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1K9</accession>
    <name>A0A8I6Z1K9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123412705</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961579.1">
        <property type="nucleotide sequence ID" value="XM_045105644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1K9"/>
    <dbReference type="SMR" id="A0A8I6Z1K9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654260"/>
    </dbReference>
    <dbReference type="GeneID" id="123412705"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412705"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035311598" description="Subtilisin-like protease" evidence="8">
        <location>
            <begin position="24"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="28"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="134"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="627"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="78848" checksum="BFF90D4CF70B6CA2" modified="2022-05-25" version="1">MDSRTAFCAALLLATLLPLSANASSKLYIVYMGEKKHDDPSVVMASHHDMLTSVFGSKDEALMSIVYSYKHGFSGFAAMLTKSQAEAIAKFPEVVSVKPNTFHQTHTTRSWDFLGLDHNQPAHQLGLLKKAKYGEDIIVGVIDSGIWPESRSFDDNGYGPVPARWKGKCQSGQKYNATGCNRKIIGARWYGRGISAESLKRDYKSPRDMDGHGTHVASTIAGGEVQGVSYRGLGKGVARGGAPRARLGIYKVCWVDQGCPDAAVLAAIDDAIHDGVDVLSLSVGLAGHEFSGSLHAVQRGISVVFAGGNDGPVPQTVTNAVPWVTTVAASTIDRSFPTLISLGNKEKLVGQSLNYNAAMNNSGFQDLVHAGRCDTESLALSNVTGKTVLCYAPAQAATTPPRAVLSRFINRTIEAGAKGLIFAQYTVNLLEILTSCEGFMPCAVVDFEIAQRIASYWEMTENPVVKISPAESIVGNGVLSPIVAMFSSRGPSPAFPGILKPDVTAPGVSILAAERDSYVFKSGTSMACPHVSAVTAMLKSVHRDWSPAMIKSAIVTTAYVTDRFGMPIQANGVPRKLADPFDFGGGHMDPERAVDPGLVYDLDAREYNKLFNCTLELSDGCKSYNLNLNLPSITVPDLMDHVILQRTVTNVGPAEATYHLVTEAPAGIDVSVEPSVINFTQGGSRRATFTVTFKTRQRVQGGYTFGSLTWSDGSTHSVRIPVAIRTVIQDFVADTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z3F5</accession>
    <name>A0A8I6Z3F5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123411060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959917.1">
        <property type="nucleotide sequence ID" value="XM_045103982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z3F5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704130"/>
    </dbReference>
    <dbReference type="GeneID" id="123411060"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0584000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0584000.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0584000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0704130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411060"/>
    <dbReference type="OMA" id="SETEFAH"/>
    <dbReference type="OrthoDB" id="625231at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="99" mass="10839" checksum="4A446AA08D7492FD" modified="2022-05-25" version="1">MAGKLYQLMARLHLAKGRASADVPKGHFAVYVGEQRKRFVIPTAYLRHPSFLVLLKRVEEEFGFDHRTGGGLTIPCSEGDFADIVGGCSSSSSPSVDYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z6I4</accession>
    <name>A0A8I6Z6I4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acidic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the plant thionin (TC 1.C.44) family. 4 C-C subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z6I4"/>
    <dbReference type="SMR" id="A0A8I6Z6I4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644940"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0645030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0645030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0645030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0644940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0645030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0645030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0645030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1350.10">
        <property type="entry name" value="Thionin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001010">
        <property type="entry name" value="Thionin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036391">
        <property type="entry name" value="Thionin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920:SF3">
        <property type="entry name" value="ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920">
        <property type="entry name" value="THIONIN-2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00321">
        <property type="entry name" value="Thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00287">
        <property type="entry name" value="THIONIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57429">
        <property type="entry name" value="Crambin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00271">
        <property type="entry name" value="THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043264881" description="Acidic protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14659" checksum="88EAC3B4904522E8" modified="2022-05-25" version="1">METISLKSIIIGVLMLGLVLQQTHIEAKSCCCSTTTRNCYNVCRVTGSSRPTCASLCGCKILEKCVPSCNRLNLVTDGDGAKSIEYCKLECMSSLCENINAVVASEEVNDVKDHCKTGCYHLYTKDYEFGEVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0I8</accession>
    <name>A0A8I7B0I8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I7B0I8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015640.1">
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
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        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15682" checksum="B46F5D599BD76DE6" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDVEVCEPEIPTRIEILLEKLNLVEKENKYLKEKLKIIEDKNMELELHVANVIDDRKMKIDAMLLKMDAMRLKMDEMRLKIRNIRKHVIDKEAWYHYAVGSIVTLVAILIAFVVAFKCLT</sequence>
</entry>
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        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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    <reference evidence="13" key="1">
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
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        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0075190.1">
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR46905:SF7">
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    </dbReference>
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    </dbReference>
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        <location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="214" mass="22297" checksum="F84B405FDA9B0F03" modified="2022-05-25" version="1">MAARPHFGRKTLLMAPAASPAAPGVTPMGEAWPAASADAERAFTWLDANAVLVLALLICGLFAAMALHVLLQCAFRVTGRAWYGANNASATPQEPVPRAGAGGGGGGRTMALPCLAYSAGLELAGSSRSECAICLAEFVRGEKVRVLPRCSHGFHALCIDRWLAARPTCPTCRQMPFAEPDESVQLAAECARPAPVVAVVRVIIHGGAGQRVEI</sequence>
</entry>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="282" mass="31846" checksum="4ED12C091BBD5221" modified="2022-05-25" version="1">MDVVVAVENLKPEDYTMPQQLVDLSPGQLHKLAELIHRQEVQKFQELKFQSYAEQQNYLKNAKEARDKVYHILGSAQEMIMEAEADKDATKQDIAKDVHEYCTKAIGTSLKFIRSYNTRLTYLDKVKTHSDDLIKQLKCLDPATQQKEAQCLALEAGRYKKAALEDAEKFEHFIPNQFSEWLKGNKIKFQDLVADDTAKPGLRGAFKTLDDTQKLKAYDNIIAGSGHGKPMVTYSYEDLGNVKIDSGTSKMNITDGLSGCRVAPMIDGLQLARLVTHNYPDL</sequence>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096010.1">
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    </dbReference>
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        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31681:SF12">
        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="157"/>
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        <location>
            <begin position="428"/>
            <end position="447"/>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
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        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="486" mass="50640" checksum="319A53B3459B217D" modified="2022-05-25" version="1">MALSTLLLPSSSSTADASDRRRGQPQQPNHHSKRKKKPPAPPPLSPAPRTPARRAGAGSTPKKSPALAAPTTGAKARLVAGKDQHGRHRRGGSSKKAPARPASSWEQLKSLLSCRSATAAARVHDPAALARGPGAGGYWGTSLCSMRDVATVDGASSAASVVVDHRDTAPLNRSSRRAHHSSSSSSAGGGGGHPSSLRGLSGCYECRAINVEPVSRRYPRPRELCPCSQCGEVFTKPDTLEHHQAVRHAVSELGPEDSGRNIVEIIFKSSWQKRDRPICHIDRILKVRNPPRTVARFEAYRDAVRARCRADAAVSAGGATRAAADGNELLRFHPAALACELGADGSTSLCASCEDGDAADGKAAAAACGVCAAIRHGFAPWAGAHPLGVRTTASSGRAHDVCGAEAATGAGWRAMLVCRVIAGQVRRKDGDGEGEEEEEGQAGAFDSVAGDDAGGAYGVLEELFVANPRAILPCFVVVYRVDADAQ</sequence>
</entry>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC123441042</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
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        </lineage>
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    <reference evidence="9" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045117568.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I7B6H6"/>
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    </dbReference>
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        <property type="entry name" value="SINL_plant"/>
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    </dbReference>
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        <property type="entry name" value="Znf_SIAH"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4"/>
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    </dbReference>
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        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
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        <location>
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        </location>
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        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024004"/>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0324180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="351" mass="36265" checksum="040659A02A62E21E" modified="2022-05-25" version="1">MGLGGGAGDGILAVMPVDAIPLTEPQIVAGGRSVGLPADLLNCHNCRFPLKPPIFKCDAEHLVCSSCRGVHGEACGGRPAVHSALADLFAASATVPCGYELYGCDAGGVVYHEAADHRRACQHAPCCCPDRAGAAGIGGCGFVGSRQMLLDHISGPDHSRPIIVVRYGQPWNLSLPLSRRWHVLVGEEDKAVAVAAGADRHRNLFLVSLGERGATTAVSLVCVRADGKAPGAPQFACKLAVESDGCRLTLESPLVCSSSLSVGLPGDVKCLPVPKDFLSGDSVPLSIHIEKLPATVALASISPAAATPPPCPAATIPPSRRSSGSSDNVAVKTVITDQSYKKRKSANPRKL</sequence>
</entry>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7Q1"/>
    <dbReference type="SMR" id="A0A8I7B7Q1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0340830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0340830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0340830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0409230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409230"/>
    </dbReference>
    <dbReference type="OMA" id="GQVCCFP"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF108">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="216"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="39932" checksum="F1F8EF5E49A31B82" modified="2022-05-25" version="1">MARSRAMCCLMPSKPSGDARRRSSAACICCIGPHHRPSGGGGLAPVDADNSSVRTPLTSCCGTGDAVLTPRTPKTPSARRLCGVRSRTPRRAQQVRRFTPAPAPAPAAAPAAPARSPVTAAASVGAAPVPTPVKAPVSAAASAGVPLVAPPPARTPVAPAAAAGALPVAPPPATTPAAAAGSAGAALAPPPARTPRTPSTPIGRTQRVCCVTSAAPAQAGNAKSKSGTARRRWLSSASKAAAQAPRNTARAASSTVRESSTNTREVAKAVHSAAPLPRAVEPAPAKEDESSVCSNEEYALLCREGFSREDVAAVTIQAYFRAHLARRAFKALRSLVRLQAVARGAYVRRQAEVAVHCMQAMARLQARVRTRQALAKPRDNDDKLLLLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B961</accession>
    <name>A0A8I7B961_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123441444</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B961"/>
    <dbReference type="SMR" id="A0A8I7B961"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0234700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1137">
        <property type="entry name" value="OS08G0387700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="187"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="936" mass="105919" checksum="088EFB22FF45A8D3" modified="2022-05-25" version="1">MAVSVVTGAINSVIDKLTGLLGDQVSSMLGKQVKLGIQYLRNELSSMKAVLLRLSDMEEHMDPHTKEWRDKVRNMSFEIEDCIDRFVQNRHNHGTSQGGLMKKAVGILKGIWDDLQISKEIEALKAMVAEESERRNRYNIDMCHPSPPPVPLDQGGLRAQYETARNLVGIDAPREKIFGWLMEQDPKPNVVVIFGIGGSGKTTLALEVYNKIQEPFDCRAFVSVSRTPNVKSLLKDILFQVNEKEYDKSEIWDLAQMILKIRNHLKDKRYLIVIDDIWSSSAWQHVKYALPIDNNKRSRMIATTRSKNVATACCADMDGHMYEAKLLSIDDSRSLFLGRIFPSNQCCPQDLREVADEILKKCGGLPLAIISIGSLLACKSRTVEVWLKIRKSVSSAVEKDSPIDQMKRILFLSYFDLPPHLKACLLYLSVFPEDYYIDSRFLIWKWMAEGLIHEENREDMEMLGESYLNELINRSMIQARKIGADGTTVKFCGVHDIVLDFITSQAAEDKFVTVLNDGKYPSKNIRWLSLHLNSSEAADLPIKDASLLRSLNIFGSSTVPRGKQLPRNCKALRVLNIEGVMKSKNHDIQHIGSFYQLKYLRITLDGKHTQRERPWDYRITGPRTAITELPEQIGNLHNLEVLDLRGCVIMKLPRTIVQLKKLVCLFLHGNSTPLPDEIGNLQCLEELSWINLCKASVRSIEGLGELTKLRALDITWISFGDGAFNDEDLMKVWASSLSKLLANSLRSLRFTQFKSSNFLDWWVFPSSSSPPPLRRLVLRGHIYFGFPTLPKQISSLINLTRLGISGVCEMGERGVNILASLPMLLSLTLRLRYGVQLGHAIGSQGFRCLVKFSLCAETCLKFEPGAMPKLQRLKLVLAKSDKYSPGGFVQGLLHLSSLKHISIGSSYFTIEHSEDWKDEIRKIISIHPNQPILDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BA10</accession>
    <name>A0A8I7BA10_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123451835</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044984313.1">
        <property type="nucleotide sequence ID" value="XM_045128378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BA10"/>
    <dbReference type="SMR" id="A0A8I7BA10"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517770"/>
    </dbReference>
    <dbReference type="GeneID" id="123451835"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0430290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0430290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0430290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451835"/>
    <dbReference type="OMA" id="AGQFFFI"/>
    <dbReference type="OrthoDB" id="687943at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009610">
        <property type="term" value="P:response to symbiotic fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000067">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF346">
        <property type="entry name" value="OS03G0791300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035254504" description="Phytocyanin domain-containing protein" evidence="12">
        <location>
            <begin position="28"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="13">
        <location>
            <begin position="28"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="128" mass="13342" checksum="37E0667D7B769531" modified="2022-05-25" version="1">MASRQVTLGAMAVVVAVATLLPATASAASYTVGDGSGWDIGIDYHAWASGKKFRVGDTLEFLYSLGEAEHNVVLVDAQSFAACTVPSNAPTLTTGDDTVSLTQAGQWFFICGIEGHCQDGMKLAVNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BA64</accession>
    <name>A0A8I7BA64_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123440108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972622.1">
        <property type="nucleotide sequence ID" value="XM_045116687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BA64"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280920"/>
    </dbReference>
    <dbReference type="GeneID" id="123440108"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0232860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0232860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0232860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0280920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440108"/>
    <dbReference type="OrthoDB" id="1922322at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="187" mass="19520" checksum="49D7D732AFCBAF2B" modified="2022-05-25" version="1">MGNFASCTLARIPGAAKGARVVLPDGGIRLVRPPTTAAELMLEAPGHFLTDARALQAGRRIEALAADEELELGGVYAAFPMKRLGSKAAPADVARLAAVFAREAHARRPASAKVAAIVVAPPDVASVAAEADIAPVRAPRLDEMAVDDEAAAAEIGELKQRISGGRLSRRRPTLETIHEESYAALVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BD76</accession>
    <name>A0A8I7BD76_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="7">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123413192</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044962077.1">
        <property type="nucleotide sequence ID" value="XM_045106142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BD76"/>
    <dbReference type="SMR" id="A0A8I7BD76"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650300"/>
    </dbReference>
    <dbReference type="GeneID" id="123413192"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0539670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0539670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0539670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413192"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000285">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000265">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="65"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="348" mass="38383" checksum="62FCE1E566B8B5ED" modified="2022-05-25" version="1">MKKPGKLALPSHDSTIGKFLTQSGTFKDGDLLVNKDGLRIVPQSEEGEAPPIKPLDNHQLSIDDLDSIKVIGKGSSGTVQLVRHKWTGQFFALKVIQLNIQESIRKQIAQELKISLSTQCQYVVTCYQCFYVNGVISIALEYMDGGSLADFLKAVRTVPEAYLAAICKQVLKGLMYLHNEKHVLHRDLKPSNILINHRGEVKISDFGVSAIIASSSAQRDTFTGTFNYMAPERISGQKHGYMSDIWSLGLVMLECATGNFPYPPRDSFYELLEAVVDQPSPSAPPDQFSAEFCSFVSACIQKNATDRSSAQTLSAHPFLSMYDDLNIDLSDYFTTAGSPLATFKQIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDM0</accession>
    <name>A0A8I7BDM0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDM0"/>
    <dbReference type="SMR" id="A0A8I7BDM0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0426030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045899">
        <property type="entry name" value="ATL71-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719:SF10">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="106"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="175" mass="18691" checksum="A866D980733E68C2" modified="2022-05-25" version="1">MITMSRDTVLICVSVAGLVVLSVLTFFCSSRRRRGHGSSSSSSHPSVVDVELGCRCATAGIDEAVLTAYPTMLYSSPARADDDDVLQPAAAPSTGDSDPALDDTTCVVCLAEYADGDELRRLPGCRHAFHRQCVDEWLRQRPSCPLCRTSPQSTAAKNSCTAGHGVSVQPRDEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEP5</accession>
    <name>A0A8I7BEP5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410150</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044958980.1">
        <property type="nucleotide sequence ID" value="XM_045103045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEP5"/>
    <dbReference type="SMR" id="A0A8I7BEP5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0706320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0706320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0706320"/>
    </dbReference>
    <dbReference type="GeneID" id="123410150"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0585770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0585770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0585770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0706320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0706320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0706320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410150"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009832">
        <property type="term" value="P:plant-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009826">
        <property type="term" value="P:unidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="7">
        <location>
            <begin position="154"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="444"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0706320.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFE3</accession>
    <name>A0A8I7BFE3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFE3"/>
    <dbReference type="SMR" id="A0A8I7BFE3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0735550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0735550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0735550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0735550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0735550"/>
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        <property type="component" value="Chromosome 7H"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="80"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="250" mass="28889" checksum="482280B5E80B7A8C" modified="2022-05-25" version="1">MKSTNRSTVAISLFVLSRSPSLFFFSLLPFSSSTMPTSFHKVLAHGTTILDVVYTNLSSEVPFFLQQWKEKRFDNAVDHEKFLGLDLEYTADQRGVAIIQLCFARHVLIFQWARSDKHCPELMDFLRSDITFASVDIRNDKLKMRHSFGIEIPAGCLVDLQTIFRLRHDRTSMAHMAVALIDDSYADMKTSFPKSQHKLWEKGPLDDINIEYAAKDAYVSYELYRKIRVVHYGQRHLEERGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BG20</accession>
    <name>A0A8I7BG20_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uncharacterized protein ycf68</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ycf68 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BG20"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0782320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0782320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0782320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0782320.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.UnG0782320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022546">
        <property type="entry name" value="Uncharacterised_Ycf68"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34890">
        <property type="entry name" value="ORF16-LACZ FUSION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10839">
        <property type="entry name" value="DUF2647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0782320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="102" mass="11203" checksum="5B4904E957604C33" modified="2022-05-25" version="1">MAYSSCLNRSLKPNKLLLRRIDGAIQVRSHVDLTFYSLVGSGRSGGGATTAPLFSRIHTSLISVWRAISRAQVEVRPQWENGAPNNASSQTKNYEITPFILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIS0</accession>
    <name>A0A8I7BIS0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIS0"/>
    <dbReference type="SMR" id="A0A8I7BIS0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0671290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0671290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0671290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0557060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0557060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0557060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0671290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0671290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0671290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF24">
        <property type="entry name" value="TTF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0671290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="93" mass="10665" checksum="6A092DB229AD8295" modified="2022-05-25" version="1">MSAIEIFGHVREVDCYPSISIAYRILFTVPVTVASAERSFSKLKLLKNYLRSTMTQERLNGLATLCIEKKLLDDIDIDPIISDFALRNVRRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXD5</accession>
    <name>F2CXD5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123402827</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356288">
        <property type="protein sequence ID" value="BAJ87506.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK358807">
        <property type="protein sequence ID" value="BAJ90018.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372840">
        <property type="protein sequence ID" value="BAK04037.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952720.1">
        <property type="nucleotide sequence ID" value="XM_045096785.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952721.1">
        <property type="nucleotide sequence ID" value="XM_045096786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXD5"/>
    <dbReference type="SMR" id="F2CXD5"/>
    <dbReference type="PaxDb" id="4513-MLOC_67670.5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559140"/>
    </dbReference>
    <dbReference type="GeneID" id="123402827"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0464540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0464540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0464540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0559140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402827"/>
    <dbReference type="eggNOG" id="KOG2088">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032757_2_0_1"/>
    <dbReference type="OMA" id="CVMCLKD"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CXD5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46398">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46398:SF4">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="1">
        <location>
            <begin position="109"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ87506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ90018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="434" mass="48985" checksum="4235C6C960DB7347" modified="2011-05-31" version="1">MSIACCLPVVECVYCLACTRWVWQRCLHTTGYDSETWGLASSGEFEPVPRLCRFILAVYEDDLETPQWAPPGGYGIDPRWVLRRRTPEHTQGRAPTYLLYLDHRHADVVLAVRGMDMAKESDYAVLLDNRIGQAGFDGGYVHNGLLKAAEWVFDAECDVLRDLLARNPGYTLTFAGHSLGSGVAAMLALLAVRDRERLGGVERRRIRCFAMAPPRCTSLNLAVRYADVINAVILQDDFLPRTDIPLEDIIKSLFCLPCLLCGNCLIATCIPESVMLRDPRRLYAPGRLYHIVERKPFRCGRYPPAVRTAVPVDGRFEHIVLSCNAISDHAIIWIEREAQRAVDLMLEHERTMKAPENQRMDGETTATRDHNEEQQAALRRAIALGISDVNLPSTYGTFDENTTHEVSEASPVVWSEWIARIFEKDESGQMVPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F2D1U7</accession>
    <name>F2D1U7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UMP-CMP kinase</fullName>
            <ecNumber evidence="10">2.7.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deoxycytidylate kinase</fullName>
            <shortName evidence="10">CK</shortName>
            <shortName evidence="10">dCMP kinase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Uridine monophosphate/cytidine monophosphate kinase</fullName>
            <shortName evidence="10">UMP/CMP kinase</shortName>
            <shortName evidence="10">UMP/CMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123425228</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.14" evidence="10"/>
    <dbReference type="EMBL" id="AK357854">
        <property type="protein sequence ID" value="BAJ89068.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964837.1">
        <property type="nucleotide sequence ID" value="XM_045108902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D1U7"/>
    <dbReference type="SMR" id="F2D1U7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123450"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123450.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123450.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123450"/>
    </dbReference>
    <dbReference type="GeneID" id="123425228"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0101710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0101710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0101710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0123450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0123450.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123450.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425228"/>
    <dbReference type="eggNOG" id="KOG3079">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="442176at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D1U7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046705">
        <property type="term" value="P:CDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03172">
        <property type="entry name" value="Adenylate_kinase_UMP_CMP_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="37"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>CMP</name>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ89068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="23542" checksum="BBF17859D8806FED" modified="2011-05-31" version="1">MGTVVDAPAAVTEKEVVAENMLGDKKVTVVFVLGGPGSGKGTQCANIVEHFGFTHLSAGDLLRAEIKSGSENGTMIENMIKEGKIVPSEVTIKLLQQAMIKNENDKFLIDGFPRNEENRAAFENVTKISPAFVLFFNCSEEEMERRLLGRNEGRVDDNIETIRKRFKVFVESSLPVIEYYDAKDKVKKIDAAKPIPEVFEDVKAIFAPFAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2G5</accession>
    <name>F2D2G5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RHOMBOID-like protein</fullName>
            <ecNumber evidence="10">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123434981</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="10"/>
    <dbReference type="EMBL" id="AK358072">
        <property type="protein sequence ID" value="BAJ89286.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971481.1">
        <property type="nucleotide sequence ID" value="XM_045115546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2G5"/>
    <dbReference type="SMR" id="F2D2G5"/>
    <dbReference type="MEROPS" id="S54.015"/>
    <dbReference type="PaxDb" id="4513-MLOC_69598.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0051180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0051180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0051180"/>
    </dbReference>
    <dbReference type="GeneID" id="123434981"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0039970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0039970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0039970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0051180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0051180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0051180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123434981"/>
    <dbReference type="eggNOG" id="KOG2289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D2G5">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF69">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="12">
        <location>
            <begin position="111"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ89286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0051180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="325" mass="35282" checksum="A97F1A8D50992E9B" modified="2011-05-31" version="1">MAGSRRDADLKEKGPPPPPPPQPGREEEREWVPWIVPVFVAANVALFAVAMYANNCPAHARGGRSGRRCVGAGLLRRFAFEPLSQNPLLGPSSATLQKLGALVWEKVVHEQQGWRLVTCIWLHAGVVHLLANMLSLVLVGLRLEQQFGFVRVGVIYLVSGVGGSVMSSLFIRDNISVGASGALFGLLGAMLSELFTNWTIYTNKAAALVTLLFVIAVNLAIGILPHVDNFAHIGGFLTGFLLGFVLLMRPHYGWAQRYVLPSSVKDVGRKFLAYQWALLAAASVLVVVGLAVGMAMLFRGVNGNEHCQWCHYLSCVPTARWSCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2R8</accession>
    <name>F2D2R8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447179</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK358175">
        <property type="protein sequence ID" value="BAJ89389.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979696.1">
        <property type="nucleotide sequence ID" value="XM_045123761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2R8"/>
    <dbReference type="SMR" id="F2D2R8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334820"/>
    </dbReference>
    <dbReference type="GeneID" id="123447179"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0279200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0279200.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0279200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447179"/>
    <dbReference type="OrthoDB" id="2016140at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035204">
        <property type="entry name" value="NDUFB11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37709">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37709:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17250">
        <property type="entry name" value="NDUFB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ89389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="95" mass="11102" checksum="C589BA778CC5A93E" modified="2011-05-31" version="1">MGFIMEFAENLILRLMEDPEQRDEAQRAHVYRMKERCERTKAAWALPLRPYGFWTFDRFNSQLSWDPQISQAAGRRDPYDDLLARHAGPAPSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2D3R5</accession>
    <name>F2D3R5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="3">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123409308</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="3"/>
    <dbReference type="EMBL" id="AK358523">
        <property type="protein sequence ID" value="BAJ89736.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958180.1">
        <property type="nucleotide sequence ID" value="XM_045102245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D3R5"/>
    <dbReference type="SMR" id="F2D3R5"/>
    <dbReference type="PaxDb" id="4513-MLOC_54179.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749810"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749810.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749810.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749810"/>
    </dbReference>
    <dbReference type="GeneID" id="123409308"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0622100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0622100.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0622100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749810.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749810.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409308"/>
    <dbReference type="HOGENOM" id="CLU_032616_0_2_1"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D3R5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF60">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ89736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="449" mass="50635" checksum="05A32BE8C8AD8101" modified="2011-05-31" version="1">MCCEMRPGFGFGFGSARRGIWRTWPALCSRNQQRFASSLDVMAATMPAPNQARKRSKVIVISGPTGAGKSRLALEVARRLGGEIISADSVQVYRSLDVGSAKPSASEMSMVPHHLIDIMHACEDYSAGMFFSDARRATQDVLGRGAVPVVAGGTGLYLRWFIYGKPNVPQSSSDIISSVWSELAGFRESGRWEEAVELLLKAGDSEARDLDTNNWARLSRRLEIIRSSGSPASAFTLPYSSFQKQQDTKLTDSPSDDATCETKELDYDFLCFFLACPRVELYRSIDLRCEEMLVDTGGLLSEASWLLDIGLQPNMNSATRAIGYRQTMEYLLHCRQNGGSSSPEEFLEFLTKFQQTSRNFSRRQMTWFRNEKIYQWVDASQPFEEIVQFICDAYNGSDALVLPESLEMKRESCLHTSKDLKTYRSENRVFLGHEDCRHILDWIRRTQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFA0</accession>
    <name>F2DFA0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444725</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="AK362567">
        <property type="protein sequence ID" value="BAJ93771.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK363450">
        <property type="protein sequence ID" value="BAJ94654.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977483.1">
        <property type="nucleotide sequence ID" value="XM_045121548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DFA0"/>
    <dbReference type="SMR" id="F2DFA0"/>
    <dbReference type="STRING" id="112509.F2DFA0"/>
    <dbReference type="PaxDb" id="4513-MLOC_60597.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0071210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0071210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0071210"/>
    </dbReference>
    <dbReference type="GeneID" id="123444725"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0057460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0057460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0057460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0071210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0071210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0071210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444725"/>
    <dbReference type="eggNOG" id="KOG0167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006348_5_1_1"/>
    <dbReference type="OMA" id="QTAEYPA"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DFA0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000317">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000354">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF274">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="272"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="416"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ93771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0071210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="682" mass="72230" checksum="5163A16D07CBA69C" modified="2011-05-31" version="1">MASAKNAAPEPSAAASSSSSYSSSAPDVEILRSLRRLARDLAAADPPAPFLRAVFASVSRRARLLVAVFDDLLLLGANGRMPRSASLCLREVLLVLQRFKAVVADCSARSRMRLLLQSDEVASRVRELQHDLATLLDILPVAELGLADDVADLLTLASRQCRRRAPGAAAEQELKASVLALIQEVEREIVPERERLEAILEEVAINDPASCSQEIEILEREIGDRLAERWTSAMIALVGLLRYAKCVLFSAATPRPLDSKVDADDDGAEPASPPPDFRCPISLDLMRDPVVSSSGQTYDRESITRWFGAGKSTCPKTGQVLTNLELVPNKALKNLISRWCRENGVAMEGCEPGKPEPAPQVTANKAAVEAARMTASFLVKKLSASFSPGSDNRVVHEIRLLAKSGSESRAFIGEAGAVPLLVPLLNSEDAALQLNAVTALLNLSILDANKKRIMHAEGAVAALCNVMGSGATWRAKENAAATVLSLSAVHTYRRRLGRNPVVAEKVVLLVRTGPPSTKKDALAALLCLSAERENVGKLVGAGAAEAALSAIGEEEIAAAVLASLAKRGGAEAIVNIDGAVAKLVAEMRRGTEWSRECAAAALVLLCRRAGAAAVSQVLAINGVEWAIWELMGSGSERARRKAASLGRSCRRWAAANAAQNAECPTTTATTTTVVSPPTVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJG1</accession>
    <name>F2DJG1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar RNA-associated protein 18 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123452712</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK364029">
        <property type="protein sequence ID" value="BAJ95232.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985357.1">
        <property type="nucleotide sequence ID" value="XM_045129422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJG1"/>
    <dbReference type="SMR" id="F2DJG1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519230"/>
    </dbReference>
    <dbReference type="GeneID" id="123452712"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452712"/>
    <dbReference type="OrthoDB" id="1935146at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DJG1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000121">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 18 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="374"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ95232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519230.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="550" mass="60554" checksum="97B5F2BCE5512DE7" modified="2011-05-31" version="1">MTSLISQNALPKRRLEKGDSSSDDEEAMGSPVASDAEAGKKPGPDGRKNDRRRKKKALQARLSQEADEMKLLESSLFGNIYAPLEFGTEAAAAVPGRGQDEGPLFFVDRSAGDDLPVYEEDLGGEDEVVDKGRKPAWVDDEEDSTVVDIVKVARLRKLRKEADERVISGREYEARLRGHHANLNPFTGWADMDRKAPLRDGDSDEEEGGVDNVLRNNHELVVKGTAKLLPGMLDYSRLVDANAQEPSSGPVNSVQFHSNGQLMLVAGLDKHLRFFQIDGKRNPKIQSIFVEDCPIQKAAFLPDGSEVILSGRRKFFYSFDLVKAAVSKIGPLTGREEKSLESFEISPDSRTIAFIGNEGYILLISSKTKQLIGTLKMNGNVRSLAFADGGNQLLSSGGDGHVYHWDLRTRKCMHKAVDEGSMSGLSLCTSQDSSYFATGSSSGIVNVYKRDEFLGGKRKPLKTIENLTTETGQMKFNHDGQILAISSGRDRNGMRLVHVPSFTVYQNWPGPRFSLQYPRCLDFSPGSGFLSIGHAGGKVLLYKLHHYQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLA7</accession>
    <name>F2DLA7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123452863</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAJ95878.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045129589.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522450.1.CDS1"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522450"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="entry name" value="DUF868_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31972">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31972:SF74">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05910">
        <property type="entry name" value="DUF868"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ95878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522450.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="292" mass="32123" checksum="B8FE05DE038B6CEC" modified="2011-05-31" version="1">MRDFPSCFGESGVQIADAASSSSSAGKGAAQNLVTCLYQTQLAARPCVVSVTWSKSLMGQGLSVAVDGLSGQSLCKADIKPWLFSKKKGSKSLDVDGGKVDIFWDLSAARFGAGPEPLEGFYVALVFDLELALLLGDMNKDAYRKTGANRPALNAAFVARKEHIYGKKIYSAKAQFCDNGQFHDLVIECDTVGLKDPCLEIRIDKKPVMQVKRLAWKFRGNQTILVDGLPVEVFWDVHSWLFGSTTTSNAVFMFQTCQAPEKSMPWSYSQIFRESQLQGLGFSLILYAWKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2DNP7</accession>
    <accession>M0YHD5</accession>
    <name>F2DNP7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">At4g15545-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123441485</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365515">
        <property type="protein sequence ID" value="BAJ96718.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372960">
        <property type="protein sequence ID" value="BAK04157.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973836.1">
        <property type="nucleotide sequence ID" value="XM_045117901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DNP7"/>
    <dbReference type="SMR" id="F2DNP7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062810"/>
    </dbReference>
    <dbReference type="GeneID" id="123441485"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441485"/>
    <dbReference type="HOGENOM" id="CLU_052741_3_1_1"/>
    <dbReference type="OrthoDB" id="5599468at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010168">
        <property type="term" value="C:ER body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080119">
        <property type="term" value="P:ER body organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058936">
        <property type="entry name" value="At4g15545-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058935">
        <property type="entry name" value="At4g15545-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47383">
        <property type="entry name" value="OS03G0659800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47383:SF3">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25972">
        <property type="entry name" value="At4g15545_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="270"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ96718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="337" mass="36917" checksum="5CA108521E11BF4B" modified="2011-05-31" version="1">MSDEGEDAPVAAVAAGFGLPEELAAVLPADPFEQLDVARKITSIALASRVGRLEAEAAGLRAQLAQRDDAAEDLRERVEQLESALALATDRLSRAEDDKETLLKEKATLSNTVSKLNRDVAKLEVFKKTLMQSLQEDDDKPNIAPKAKLTEASSFSPAPSVGDDDSAFPTSKSSQLFETASSASEESSHAEPDVARPPRSHVYMPSYNSTPKLTPPDSPPRGYAPLSPPRRHSISIASMNRLDDKSSVFSSNHSSMTSPFDTPSQTGRTRVDGKEFFRQVRNRLSYEQFSAFLANVKELNAHKQTREDTLRKADAIFGPENSDLYTIFESLITRSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTM4</accession>
    <name>F2DTM4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123394996</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK367242">
        <property type="protein sequence ID" value="BAJ98445.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945840.1">
        <property type="nucleotide sequence ID" value="XM_045089905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTM4"/>
    <dbReference type="SMR" id="F2DTM4"/>
    <dbReference type="PaxDb" id="4513-MLOC_63169.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0473960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0473960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0473960"/>
    </dbReference>
    <dbReference type="GeneID" id="123394996"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0392570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0392570.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0392570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0473960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0473960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0473960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394996"/>
    <dbReference type="HOGENOM" id="CLU_000288_22_1_1"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DTM4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001678">
        <property type="entry name" value="Calmodulin-binding receptor kinase CaMRLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000466">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF20">
        <property type="entry name" value="OS08G0276400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090783" evidence="15">
        <location>
            <begin position="27"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="526"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ98445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0473960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="832" mass="89222" checksum="DF5E6B4F29351696" modified="2011-05-31" version="1">MGRGGAHGVASLFLAVAAAVCCAAAAQEPNTDAYFVARFYAKMGLASPPSGTGAVCSWPGVSCDGEGRVLALAAAGMGLSGPIPEDTVGKLARLRELDLSANRLTALPNDLWELGASLRTLNLSRNAIRGALPNNVANFARLQVLDVSHNAFSGALPPALASIAGLQVLNASHNQFQGQVPSAGEGLVAMDLSGNALDGDLPDLSPLRSLAYLNLSVNQLHGSIIGAFQEQLRAIDLSNNRFSRLNFSSGYSGTSLMYLDLSSNELLGEFGLPGRFRNLRHMNLAFNQLSTNNLLASIAETSALEYVNLSSTGLHERIPGVLASKLVGLSVLDLSRNNISGVVPDMSALPLRVLDLSVNNLTGEIPVSLVKKLASMDRFNFSYNNLTVCASELSPEAFAAAFARSRNDCPIAVNPDSIKKNGAHRKGMKLALAIVLTLFFSVLALLCLALVCRRPRKKRGDTFPADKQRSSSFKEEPGTSGPFAFQTDSTTWVADVKVATSVPVVIFEKPLLSFTFADLLAATSNFDRGTLLAEGRFGPVYTGFLPGGIQVAVKVLVHGSAMADEDAARELERLGRIKHSNLVPLTGYCLAGGQRIAIYEYMENGNLHNLLHDLPLGVQTTEDWSTDTWEDNNGGMATENITPEGTAAWRFRHKIALGAARALAFLHHGCIPQIVHRDVKASSIYFDYAMEPRLSDFGLSMIAGTSTDGDHSPGYVPPEFSDPENATATSKSDVYSFGIVLFELITGKKPLGDEYPDQKEASLVSWARAMVKANQGSSIIDPKIRDTGLERQMDEALRVAYLCTAELPSKRPTMQQIVGLLKDIEPRVTDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWS8</accession>
    <name>F2DWS8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123395220</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probable oxidoreductase that may play a role as regulator of mitochondrial function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with COX5B; this interaction may contribute to localize PYROXD2 to the inner face of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK368346">
        <property type="protein sequence ID" value="BAJ99549.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946107.1">
        <property type="nucleotide sequence ID" value="XM_045090172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWS8"/>
    <dbReference type="SMR" id="F2DWS8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421470"/>
    </dbReference>
    <dbReference type="GeneID" id="123395220"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0350890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0350890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0350890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395220"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="7">
        <location>
            <begin position="52"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ99549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="586" mass="63062" checksum="38CD14EC0810F505" modified="2011-05-31" version="1">MSPAATRILLAGARRRGRGLSTSSDPSPSLSPSELPRGKRWDAVVIGGGHNGLAAAAYLARAGRSVAVLERRGVLGGAAVSESDLVPGFRFSRCSYLLSLLRPALIRELELERHGLKLLPRSPSSFTPCLDGRYLLLGPDAELNRSEIGKFSKKDAEAYPRYEEQLEKFCKLMDFVIDSPPPELRQLYHASMVDRMKDKVDKSVFWSKLLGIVMQQGQKDMVNFFDLLLSPASKILNNWFEGDVLKATLATDAVIGTMAGANTPGSGYVLLHHIMGETGGQRGVWAYVEGGMGSVSSAISKAALEAGVQIVTNAEVSQVMVDETTGKVQGVALADGTELHSSVVLSNATPYKTFVDLVPANTLPEEFLCAIKTADYSSATTKINVAVDALPQFRCCKNINPAGGREHMGTIHIGSESMEEIDVAYKEAAGGFSSTRPVIEMTIPSVLDKTISPPGQHVINLFVQYTPYKLSEGSWQDPAVRKSFAERCFSLIDEYAPHFSSSVIGYDMLTPPDLEREFGLTGGNIFHGAMGLDSLFLMRPAKGWSDYRTPVKGLYLCGSGAHPGGGVMGAPGRNAAAVVLDDLKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2E119</accession>
    <name>F2E119_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123443630</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="AK369840">
        <property type="protein sequence ID" value="BAK01041.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976041.1">
        <property type="nucleotide sequence ID" value="XM_045120106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E119"/>
    <dbReference type="SMR" id="F2E119"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0261980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0261980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0261980"/>
    </dbReference>
    <dbReference type="GeneID" id="123443630"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0261980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0261980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0261980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443630"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF515">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="126"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAK01041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0261980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="325" mass="36581" checksum="E2F8F6DE5B1B6CDA" modified="2011-05-31" version="1">MMMTRASMGEMDGAALDEVVRRLVEGGRGGRQVQLSEAEIRQLCVEAKRVLLSQPNLLRIPAPVKICGDIHGQFVDLLRLFDLGGYPPTSTYLFLGDYVDRGKQSLETICLLLAYKVKYPDKVFLLRGNHEDAKINRVYGFYDECKRRFNVRLWKIFCDCFNCLPMAALIDDKIFCMHGGLSPELNCLDQIKDIERPVEIPDYGLLCDLLWSDPSSDTQGWGESDRGVACTFGADKLVEFLEKNDLDLICRAHQVVEDGYEFFAERRLVTIFSAPNYCGEFDNAGALLSIDESLMCSFQILKPKETGAPHSRKPISNKAPTVDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2E328</accession>
    <name>F2E328_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123447601</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Flower</tissue>
            <tissue evidence="16">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370551">
        <property type="protein sequence ID" value="BAK01750.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK376335">
        <property type="protein sequence ID" value="BAK07530.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980145.1">
        <property type="nucleotide sequence ID" value="XM_045124210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E328"/>
    <dbReference type="SMR" id="F2E328"/>
    <dbReference type="STRING" id="112509.F2E328"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338400"/>
    </dbReference>
    <dbReference type="GeneID" id="123447601"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0282300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0282300.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0282300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0338400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447601"/>
    <dbReference type="OMA" id="TRSCCEP"/>
    <dbReference type="OrthoDB" id="1914959at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="48"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAK01750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAK07530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="150" mass="16236" checksum="8A01AC7806F27913" modified="2011-05-31" version="1">MAPKAEKKPAAKKPVEEEPAAEKAEKTTAGKKPKAEKRLPAGKSAAKEGGKAKKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2E3P8</accession>
    <name>F2E3P8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123402830</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370771">
        <property type="protein sequence ID" value="BAK01970.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952723.1">
        <property type="nucleotide sequence ID" value="XM_045096788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E3P8"/>
    <dbReference type="SMR" id="F2E3P8"/>
    <dbReference type="STRING" id="112509.F2E3P8"/>
    <dbReference type="PaxDb" id="4513-MLOC_13455.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0602620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0602620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0602620"/>
    </dbReference>
    <dbReference type="GeneID" id="123402830"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0499610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0499610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0499610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0602620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0602620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0602620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402830"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E3P8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000003">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017209">
        <property type="entry name" value="Memb_At2g17000_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="709"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="738"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="10">
        <location>
            <begin position="757"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="165"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAK01970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0602620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="938" mass="104757" checksum="48E9AFFCC69819E9" modified="2011-05-31" version="1">MDQVQRKSSLRSLSMSDEQAMAPGLGERREVVLKIDGNGNGNGPAPFSVPGADVGGAAGNAGAKPTAGRTLSTAASSPTKGWDDGSYDFWKNEGGGKGGAPAPRVEDFSFKNRPAQPPPSSQASSPSLSPKQPVVDAAAEDPPTRLIGNFLRKQKAAGAELSLDLDLEMDDIGRSSHPSLSNSRERETPRVSFKDRQSSSSSSSDSDTAGGRRRAGDDGTRNTSTSTPAGKGPLLRAKTRSRLMDPPPQSPMAPPAVDEERKSSARPPKSGQFPSGRMTGKSGQSPSGRKSGVIGKSGPMEEEEDDPFIDDDIPDDFKRGKLDALTILQWVGLVLIIGALVCSLTIKPLSRKKVWELHLWKWELLVFVLICGRLVSGWVIRIVVFCVERNFVLRKRVLYFVYGVRGAVQNALWLGLVLASWHFLFDENVQRETNTAVLPYVTKVLFCFLVATLIRLVKTLLLKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPLVDEDYVLAEVRELQRAGATIPKELRGALPAKNLSGQKSIRISGLISKGDQSSRQLSKEKKQREIDEGITIDKLHRLNQKNVSAWNMKRLMKIVRFGTLTTMDEQIQQATGEGDESATQIRSEYEAQVAAKKIFHNVAKPGSKYIYLADMMRFMRQEEAIKAMHLFEGAQEHCRVSRRSLKNWVVNAFRERKALALTLNDTKTAVNKLNQMCNIVVGLIVSALWLLILGIATTHFFVFISSQLLVAVFVFGNTMKTIFEAIIFLFVMHPFDVGDRCEIEEVQVVVEEMNIMTTVFLRYDNLKIYYPNSVLATKPIFNFYRSPDMGEGIDFSIHVATPVEKLALMKERILRYVDGKKEHWYPGAMVVLRDVDETNKLKVSIWLRHTLNFQDMGMRFVRRELVLQEMIRVLKDLDIEYRMLPLDVNVRNVPPLQSTRMPTTWNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2E5C6</accession>
    <name>F2E5C6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK371350">
        <property type="protein sequence ID" value="BAK02548.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E5C6"/>
    <dbReference type="SMR" id="F2E5C6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0432560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0432560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0432560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0520260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
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        <property type="term" value="P:ribosome biogenesis"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
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    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
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        <property type="entry name" value="GTP1-OBG_CS"/>
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        <property type="entry name" value="GTP1_OBG_dom"/>
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        <property type="entry name" value="GTP1_OBG_dom_sf"/>
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        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF39">
        <property type="entry name" value="GTP-BINDING PROTEIN OBGC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="82"/>
            <end position="291"/>
        </location>
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    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="292"/>
            <end position="504"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK02548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="514" mass="54342" checksum="C4ADDC0AEAC9C9CD" modified="2011-05-31" version="1">MATLLLPSHAASHHRRAVFSGSARHHPLPLPDLLGGALRAVAGRRRRPIAVACRAASAAARAKAPPSANAGPAPQALAKEAHKYFDHAVVSVRAGDGGHGAVLNMPPGTSADAAPARPRGGGRAADKGKAKRGSGKKVSFKRNYDGSVSLPVGGHGGDVVLYADEAEETLLGFHSKARLCAKRGGNVGATGTLSSRMHNGFAGETLRIPVPVGTVVRRKKGSVLADLAHPGDEVLVARGGQGGISLIDAPDYKRGKAMALSPNVMRDVTDKVLTHGQPGEEISLELILRVVADVGLVGLPNAGKSTLLSAITLARPDIADYPFTTLMPNLGRLGGDPTLGALQFSSGATLADLPGLIEGAHLGKGLGRNFLRHLRRTRVIVHVVDAAADDPVNDYKIVREELRMYNPKYLERPYVVVLNKIDLPKAQDRLSSLALEISSVGCEESHDKNASKEKLNVNFNRHHMSEDDDKELGDYPRPQAVIGASVLRRIGIDEMLKEIRTALGKCSDHMLPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F2EC79</accession>
    <name>F2EC79_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430391</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAK04951.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174080.1.CDS1"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.2HG0143890"/>
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        <property type="entry name" value="OS06G0632400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK04951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8821" checksum="0F7614B95E6DF25F" modified="2011-05-31" version="1">MGRRSSFFCAIFSFSRRSRRYAYVDDQDSDWEQPPAPGLRKVRSSDDDSGWWVGERDVDQKAADFIASFHQRSLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2EGQ8</accession>
    <name>F2EGQ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123398233</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Flower</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the two pore domain potassium channel (TC 1.A.1.7) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375335">
        <property type="protein sequence ID" value="BAK06530.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948662.1">
        <property type="nucleotide sequence ID" value="XM_045092727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EGQ8"/>
    <dbReference type="SMR" id="F2EGQ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0471980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0471980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0471980"/>
    </dbReference>
    <dbReference type="GeneID" id="123398233"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0471980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0471980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0471980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398233"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000150">
        <property type="entry name" value="Two pore potassium channel c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000102">
        <property type="entry name" value="Two-pore potassium channel 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF282">
        <property type="entry name" value="TWO-PORE POTASSIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="160"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="288"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAK06530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0471980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="455" mass="49813" checksum="EA9F5BA633E8E015" modified="2011-05-31" version="1">MDTDPLLPTTAPQLLHPLPEHAPVSNFDAAPPPSPASSYKDRLIFGPHHPPPPPPPPPPPPPSGHHHYRRISVGHADPFRDYDHPSCSSSPPSDEEAPPQPTTPSLFNLISGRTNLHRSRTAPAMAPLSAAVLAASAEADHHNPPPPPRRPAIVLHAFLFLLAYLALGVTFYAAFPANFTSSAGPTHPVVDALYFCIVTLCTIGYGDITPASPAAKLFAISFVLIGFGFVDILLSGMVSYVLDLQEHLLITAIKNPRSARKHRHNYIFDIKKGRMRVRMKVALALGVVAICVGVGATVLRKVESMGWLDAVYLAVMSVTTVGYGDHAFRTLHGRLFASAWLLVSTLAVARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEFVVYKLKEMGKISDKDIRMIVDQFQRLDSGNCGKITLSDLLQSHHLGHEPRDKKRGKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2EIS9</accession>
    <name>F2EIS9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430497</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK376056">
        <property type="protein sequence ID" value="BAK07251.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970300.1">
        <property type="nucleotide sequence ID" value="XM_045114365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EIS9"/>
    <dbReference type="SMR" id="F2EIS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180480"/>
    </dbReference>
    <dbReference type="GeneID" id="123430497"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0149180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0149180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0149180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430497"/>
    <dbReference type="OrthoDB" id="10261027at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF287">
        <property type="entry name" value="OS12G0605900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="87"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK07251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="397" mass="44310" checksum="36AB4770D259D583" modified="2011-05-31" version="1">MAAEKEEVAVESKIVEKEKPMAQRSGFLGGGGKMEAETYIRADMIDLEELDLEKQRESIAKTLALEKQRQKDNHRPMAPWEIDLAKLTVHRRIAPGTFGSVYRATYDGKDVLAKLLDWGEDGFMPETEIAIQREALRKEVIVWKELDHPNITKFIGASMGTIDLTIPPESGECTAPPDPPERACCVVVEYLSGGTLRQHLYANRNDKLTYEAVVELALDLARGLAYLHSKDIVHRDVKAENMLLDSKGTLKIADFGVARVQAKNPQEMTGMTGTPGYMAPEVILGKPYNRKCDVYSFGICLWAIYCCDMPYYPNKSFGEASSDIVHKNLRPKIPRCCPAPMANIMKSCWQADPEKRPDMLDVVQLLDGLDTTQGGGMIPEEKTPGCFCFLRPRRAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL78</accession>
    <name>F2EL78_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123407686</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Seed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK376906">
        <property type="protein sequence ID" value="BAK08100.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956808.1">
        <property type="nucleotide sequence ID" value="XM_045100873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EL78"/>
    <dbReference type="SMR" id="F2EL78"/>
    <dbReference type="PaxDb" id="4513-MLOC_76536.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681560"/>
    </dbReference>
    <dbReference type="GeneID" id="123407686"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0565850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0565850.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0565850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0681560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407686"/>
    <dbReference type="eggNOG" id="KOG2352">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="YEDNVIC"/>
    <dbReference type="OrthoDB" id="411785at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EL78">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000300">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF59">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK08100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681560.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="484" mass="52112" checksum="DF62FCFEC94B7403" modified="2011-05-31" version="1">MAVCTELNQLQHIEPSRFISFSFPNPLLHDASNPYADADADASDHAELLRVAVLDSPAAATPSPPAPRTAGMLVPAGRHRDWIFSTRAGQLHLLLSSRSQWTFSRLILVGPELSTPSPRVVSCAAARPDPDPARARLLPLFLALCPRAAFGNGAIPYVPLLSFHDELLRLVSVQVVAGPVVGEMLVEDVAVDCAPGPAELRRRLRFKRMPCLVQTQVRLARPMSAAAAASSSLEALEEAPASSLQPEVGGPLVQPYLQAMVAGLALIGSSVEEQARSAARPRCLCAGIGGGALPMSIRMGLGFDVLGVEADCVVLDVARNYFGLVEDEFLHVRVGDAIQMIQAFAHPDEPDSKFSAVMVDLDSPDAMCGVSAPPLEMTHRSTFLAARRILDDNGVLVLNVIPPAADASFYKGLIDVLHQVFFELYEIDVGNGENFVLVARVSPTKSTILDSSRLFQTELRKLTGDFLERIKKVEIPSYSLCLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>M0UK85</accession>
    <name>M0UK85_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPI transamidase component PIG-T</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123448071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980779.1">
        <property type="nucleotide sequence ID" value="XM_045124844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UK85"/>
    <dbReference type="SMR" id="M0UK85"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0352760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0352760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0352760"/>
    </dbReference>
    <dbReference type="GeneID" id="123448071"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0294280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0294280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0294280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0352760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0352760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0352760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UK85">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015096106" description="GPI transamidase component PIG-T" evidence="2">
        <location>
            <begin position="27"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="622"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0352760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="71436" checksum="E71BE51692D2F5C5" modified="2013-04-03" version="1">MVPPSTARRLCSLSLLLLLLGAAASATADAAAAEEFTEELLLRPLPDRKALAHFHFRSSAPPASAAGRHQHLFPKAISQLVQKFHISELELSFTQGRWNYEQWGGYDPMSTNNAKPPGVELWAVFDLPLAEIDATWKNLTHTLSGLFCASINFLESSTAFSAPRWGFKLNEGNLRYGALPREAVCTENLTPWLKLLPCRDKAGIASLLYRPSVYKGYYHSQKLKLKSSQSLGIILDQTLTVVLQPNTLSGKQLHPTGGQLQPSWSMEHLFNRKLSGKCLVSKSSRVFVELEKGIVDKAGSEVSWNNDLFVLSTAPDRSLKELDNLEVQSSSLYEYDVNNYNNDKPLNVGITWKLPLIWSCTRAPYHASRFLMGSGNERGSIAMSFLSAGLDKQLVDSSNDCSIKAVVFQVVPWYVKVYYHSLEVFIDGNSMAISEVVDKIHVTPSEDKLLPGTMEMLLKFPCSMQLATLTLDFDKGFLHIDEYPPDANQGFDIPSALVSFPEFNSSRSYPEADPLFVSPLLENFKEDGVVKSYTEVLLVPLTTPDFSMPYNVITFTCTVLALYFGSLLNALRRRIGEEERVLKKADKRQGLIPLLIAKLRGQKVNPSEPESMSRASLARSKLLLKVVFVAVVAIVFHYLSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y3X1</accession>
    <name>M0Y3X1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123425152</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964767.1">
        <property type="nucleotide sequence ID" value="XM_045108832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y3X1"/>
    <dbReference type="SMR" id="M0Y3X1"/>
    <dbReference type="FunCoup" id="M0Y3X1">
        <property type="interactions" value="516"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0Y3X1"/>
    <dbReference type="PaxDb" id="4513-MLOC_65478.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0121630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0121630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0121630"/>
    </dbReference>
    <dbReference type="GeneID" id="123425152"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0100250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0100250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0100250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0121630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0121630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0121630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425152"/>
    <dbReference type="eggNOG" id="KOG0765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Y3X1"/>
    <dbReference type="OrthoDB" id="250329at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y3X1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF5">
        <property type="entry name" value="OS01G0329400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="4">
        <location>
            <begin position="36"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="4">
        <location>
            <begin position="132"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="4">
        <location>
            <begin position="239"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0121630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="35782" checksum="E52D6C584128ED09" modified="2013-04-03" version="1">MAAAAAAAADTSAASTTGLALSEASINWERLDKTKFHVIGAFLFTAQQGVLHPTAVVKTRMQVAEGGLSHMSGFVVFRKILRSDGIPGVFRGFGTSAVGALPGRILALTSLEMSKEMAFKYSEHFDMSDASRIAVANGVAGLVSSIFSSSYSVPLDVVCQRLMVQGLPGMQTYRGPFDVINKVVRSEGLRGLYRGFGITLLTQSPASALWWSSYGGAQHAIWRSLGYGIDTQKKPSHSELIAVQATAGTIAGACSSIITTPIDTIKTRLQVMDNYGSGRPSVMKTTRLLLREDGWRGLYRGFGPRFLNMSLWGTSMIVTYELIKRLSVKPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287NU71</accession>
    <name>A0A287NU71_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">FYVE finger-containing phosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">PIKfyve</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylinositol 3-phosphate 5-kinase type III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123448528</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044981386.1">
        <property type="nucleotide sequence ID" value="XM_045125451.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981388.1">
        <property type="nucleotide sequence ID" value="XM_045125453.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981389.1">
        <property type="nucleotide sequence ID" value="XM_045125454.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981390.1">
        <property type="nucleotide sequence ID" value="XM_045125455.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981391.1">
        <property type="nucleotide sequence ID" value="XM_045125456.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A287NU71"/>
    <dbReference type="STRING" id="112509.A0A287NU71"/>
    <dbReference type="PaxDb" id="4513-MLOC_57352.5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369960"/>
    </dbReference>
    <dbReference type="GeneID" id="123448528"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0308050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0308050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0308050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0369960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448528"/>
    <dbReference type="eggNOG" id="KOG0230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287NU71"/>
    <dbReference type="OMA" id="VGPKFLF"/>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287NU71">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000384">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase FAB1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000006">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase FAB1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF3">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="317"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="865"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1331"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1331"/>
            <end position="1344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1839" mass="205640" checksum="924E272B714CDA21" modified="2017-11-22" version="1">MEASDRTFADVVKLLTSWLPRRSKPDNVSRDFWMPDHSCRVCYDCDAQFTIFNRRHHCRRCGRIFCGKCTGNSVPVPSGPDKSNDEGDRIRVCNFCFKQWEQEQIAAVKQILPVLSPSLSEASLFSTKSTITINSVSIPAASYSTGNYQHMARASNIGPPPPKFSQDKVSHSMQDSHVPEISMSTISNRDDSTLMQFGYYTNRSDDEDEEFGAYCSDSQVQHKQHNNQYFRSDEFDEFDASYKTTISKTIGVNVIPKELSPCAVDHGFTSTLPVTKVEDEPELDNSSDCGAASSIYALETADTNSVDFEMNDYFWIPPEPEDKEDELETDLFDDDDDDDEPVSDGGRCYLQSSSSFGSGEFRSRDRSTEEHKKVMKNVVDGHFRALISQLLQVENITLEEGDDMGWLEIVTSVSWEAANFLRPDTSQGGGMDPGGYVKVKCLASGHRSESTVVKGVVCKKNVAHRRMTSRIEKPRLLLLAGALEYHRVTNQLSSIDTLLQQETDHLKMAVAKIVAQKPNLLLVENTVSRYAQDLLLEKNISLVLNIKQSLLQRIARCTGAQIVPSIDLLPSQKLGYCELFHVDKHIEHSVTSGNKTKKTLKNMMFFEGCAKPLGCTVLLKGGSMDELKKIKHVVQYGIFAAYHLALETSFLADEGATLPEFPLRSPLTVTLPDKRSTVDNSISMVPGFTINISNSRQATDSFEHLGTDNVISTDPGGTAVVEPPVDSDCLTSQKANSHSFGPSCTSGCSFNSRNGDKLEVTATADSVSMSSITTHDVPTDPSHIYSTVGKKCVYSGDYHADYSTSSDGKTVRADSINTNNHQSTSQVSTNITNYSNLKEPLEGSNALPNVRIVNANNLLAVQPMSSPAVQNQETSQGNDITSNKTEIVPSDHQSILVSLSIRCVWKRSICERSQLLRIKYYGNFDKPLGRFLRDYLFDQGYQCRSCDKPPEAHVHCYTHRQGSLTISVRKLTDVVLPGERDGKIWMWHRCLKCPWSDGFPPATHRIVMSDAAWGLSLGKFLELSFSNHAAASRVASCGHSLHRDCLRFYGFGKMVACFRYAPINVHSVHVPPHKLYFTHQPLDWIQKEVNEVIDRAKVLFDEVSRTLHLISEKKAHSSSLNVECTNYIAELEGMFRKEKLEFEGCLNKVLKKEVPKCQPDILEINRLRRQLLFHSYLWDQRLLFAARSDRNRYELSNTRQGDKETIHSVDSVADPNVREQLQSENSGSVAANKDVKYAENLQVSICGQNCSGVDPSNSYCNLDQRIPTSESDFLQRNIQTPLYTSVSVNGDSVPFEPDLVARRTLSEGQFPSLLDVSNALDAKWTGENDPVTSKITLPDSTASSEDSEEHISDTTPSYASVLLNKLGDSAEDHSNWIGMPFLQLYRSLNKKWSRSKRFDALIEYTPVYISFLRAVERQIGPKFLFPIGINDTVIGVYDDEPTSIISYALASHEYHLQLSDELEREATDNSPPLCDSRSVSLTESVDETTSELLRSVVSTDDNIVSIPGSKNPSTSDPLVYGKVTHIKVNFGDEGPLEQVKYTVICYYAKQFDALRRICCPSERDFVRSLSRCKKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFMKFAPDYFKYLSESIGTGSPTCIAKILGIYQVKSLKGGKEVRMDVLAMENLLFERRVTRLYDLKGSTRSRYNPDSNGSDKVLLDENLLEAMPTSPIFVGNKAKRLLERAVWNDTAFLASIDVMDYSLLVGVDEKKHELVMGIIDFMRQYTWDKHLETWVKASGILGGPKNVSPTVISPKQYKKRFRKAMSAYFLVVPDQWSPAAIIPSKQVADSGQDSDQFVCTGSSSTLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287T3S8</accession>
    <name>A0A287T3S8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287T3S8"/>
    <dbReference type="SMR" id="A0A287T3S8"/>
    <dbReference type="FunCoup" id="A0A287T3S8">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0449480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0449480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0449480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540510"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287T3S8"/>
    <dbReference type="OMA" id="SKGRWML"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287T3S8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="194"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="360" mass="38307" checksum="7CF48A6915E74394" modified="2017-11-22" version="1">MLVDQVCAESMGWMELLKPVAAMIAFDTLFAVMTVLVKKALDGGLNPVVLIALRQLVGAAFLAPIAYFKERNVRPRFTKEIFAYLFMSALLGALFAQYLFFLGLSYTTATLAATVSNMTPVFTFLIAIPLRSETVDVRSKAGLAKIAGTLASVGGAILLSLYKGAALTHASSSAQEHAGNGTTGTSGSKGRWLLGSALLLLNCITFSLWMLLQGKLTKKYPAVISSTAFMALFSSLQAGALAVTTQRRLSVWLLRGSIQIATVLFAGVGVSGIGYVLMTWCIEKRGPVFTAGFLPLIQIIAGVLDLLVLHEQLYVGSVVGAALVIGGLYLLLWGKSKEASSAAVLSQKGLEEDKETQENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287XFM2</accession>
    <name>A0A287XFM2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287XFM2"/>
    <dbReference type="SMR" id="A0A287XFM2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287XFM2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF86">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="577" mass="64208" checksum="0D0F81AF0119ABFA" modified="2017-11-22" version="1">MKNLSVLVLDQNPVSLWTISKTLARFNFKVLPFLTPEEALDFLKRGVAKDEELDLIVAEVHPGNTEMGTLVLFHHILNELEVPLITMCAYDEAVSAHMTLGTCFNVIKPLDTKTVNFLRMRALQNRSIKNHRSETEDEEQYALNLNVYSDNLGRFIWSSELHEKFLQAVEVLGASATPRKIHQYMNANDLNLTIQHVASHLQKHRLRAQKLSHDEECYQHYASTEELSEMIAAAYKADSAKPNNHQATTQTQITHGVASAIWDKYPGMVWPHVKGSSTASAMWYSNPGKPRGQVGESSARARVSQTAASPPPVLLHGTKSIWDRYEESLQNYNESLSYKRGVLPIKSKALHGHGRRVFINLEKEETSRTETAGKIVMNLQRDDMQKATTDEVHAAITPQEGTMDEVHAAVTLQDDTMDGAHAAVTPHEVNEVPVAAMGAGDLMDLGSGSLLDGMDNYHPIAENAQSEPFSDWAWAEVQKFWTNQMEGQGQEQQGLELVDLLQVDGISPEELLQEDESWNQALQQANPANVVVDEPMAEEPVAGDAPVYDPVNQSGVADNMFSVWSPQFAGDDYGMPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WEL5</accession>
    <name>A0A8I6WEL5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WEL5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0011090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0011090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0011090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0008250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0008250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0008250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0011090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0011090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0011090"/>
    </dbReference>
    <dbReference type="OMA" id="HTKRDYG"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF3">
        <property type="entry name" value="CASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="11"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0011090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="180" mass="19506" checksum="0E673C8562F86E74" modified="2022-05-25" version="1">MAVSRRTGWIAAGLLARLLTMAVLLMSVRFVLANYVHWDYAQDNYKLQSYTYVVASAVVGTAGSLLQIPVAMYLLCKSKRAIPSAIILDISMHADILISVVLASGVGAGFGATNDVLRYVRAGDWEREGKQQEQQALINYLNRAFIPVVFLLVGMVLSICATVVSARLRARATNDAQGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WF46</accession>
    <name>A0A8I6WF46_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123420070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044963203.1">
        <property type="nucleotide sequence ID" value="XM_045107268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WF46"/>
    <dbReference type="SMR" id="A0A8I6WF46"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0089900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0089900"/>
    </dbReference>
    <dbReference type="GeneID" id="123420070"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0073960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0073960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0073960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0089900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0089900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0089900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123420070"/>
    <dbReference type="OrthoDB" id="728759at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000473">
        <property type="entry name" value="Caffeic acid O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000061">
        <property type="entry name" value="Caffeic acid O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="137"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="360" mass="39591" checksum="4ED3F14535D4CB38" modified="2022-05-25" version="1">MGSTATKKDAASDEEACMYALQLATSSILPMTLKNAIELGMLETIVSADGKALSPSEVAAQLPCKANPDAPAMVDRMLRLLASHKVVTCHMEKGEDGLLTRRYSPAPVCKWLTPNEDGVSMAPMLLMVQDKVLMDCWYYLKDSAVDGGLPFNKAHGTTVFEYQGKDTRFNSVFNEGMKGHSSIIIKKLLELYMGFEDISTIVDVGGGVGTTIHAITSKYPHIRGINFDLPHVIAEAFQSLHVQHIGGDMFEKVPPGDTIIMKGILHDWNDEHCMTLLRNCYDALPTQGKVVVVECILPVSPEAKLGEQVVLNVDMIMLAHTPSGKERYLNEFEELAMGAGFSRVNTTYIYAESWAIEFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WHG8</accession>
    <name>A0A8I6WHG8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123413530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962404.1">
        <property type="nucleotide sequence ID" value="XM_045106469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WHG8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069240"/>
    </dbReference>
    <dbReference type="GeneID" id="123413530"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0055620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0055620.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0055620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413530"/>
    <dbReference type="OrthoDB" id="761790at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040267">
        <property type="entry name" value="EID1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31348">
        <property type="entry name" value="EID1-LIKE F-BOX PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31348:SF3">
        <property type="entry name" value="EID1-LIKE F-BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069240.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="295" mass="31109" checksum="CB23EEC205DB8D60" modified="2022-05-25" version="1">MSEGTRKMRRLRVASGGGGGSSKANASGAIADEWSSGGRGIGARIRGVNVGILDEQVLVLVFRALNWDPQALCSVARVSRRLRAVAERVLWRELCASRAPRMVSALTTGPSASSGSGRVGGGWPALAKLLLFCCGAAGAGVRGHFAPVSRFSKTSGRSFLSRRCGGDLLYVSDPCEHAAAADDEDVGAYRGVFRGFMRSRTRACLVGGRAPLDPCVRCPYCGARVWSVTAAGLAPRSACRRLGAHEGRLEYFVCVSGHLHGSCWLARLSSSDGEDSESDDGRFTAADSDDGHMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM21</accession>
    <name>A0A8I6WM21_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter D family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123437727</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971667.1">
        <property type="nucleotide sequence ID" value="XM_045115732.1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0003690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0003690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0003690"/>
    </dbReference>
    <dbReference type="GeneID" id="123437727"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0002840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0002840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0002840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0003690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0003690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0003690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123437727"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF54">
        <property type="entry name" value="OS05G0107600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="189"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="453"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="765"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1102"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0003690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1327" mass="148409" checksum="92CA70DD45556700" modified="2022-05-25" version="1">MPSLQLLQLTERGRGLLASRRRTLAVVSGALVAGGALAYARSGRGQRRRGRPEAAAANDGGDALGRNGERLGHSGTDGRLAGTTKRRKSALKSLHFLAAILLKKIGPSGTRYLLGLMLTAVLRTAVGHRLAKVQGFLFKAAFLRRVPTFTRLIIENLILCFLQSAVYQTSKYLTGSLNLRFKKILTDLVHADYFQNMVYYKISHVDHRISNPEQRIASDIPKFSSELSELVQDDLAAVAEGLIYTWRLCSYASPKYMLWILAYILVAGGAIRNFSPAFGKMKSTEQHLEGEYRQLHSRLRTHAESVAFYGGEKREEYHIMQRFRALVGHLKHVLHENWWFGMIQDFFLKYFGATVAVVLIIEPFFSGDLRPDSSTIGRADMLSNLRYHTSVIIALFQSLGTLSISSRRLNILSGYADRIRELLDVSRELSGVRDRSLNHSSSVGNYISEANHIEFSGVKVVTPAGNVLVDDLTLRVETGSNLLITGPNGSGKSSLFRVLGGLWPLVSGHIVKPGVGSDLNKEIFYVPQRPYTAVGTLREQLIYPLTADQETEPLTYDGMVDLLKNVDLEYLLERYPLDKEVNWGDELSLGEQQRLGMARLFYHKPKFAILDECTSAVTIDMEERFCKKVRAMGTSCITISHRPALVAFHNIVLSLDGEGGWDVQHRRDDSSFYTEDSELTSLETERKSDALIVQRAFMNRAKSNASLRSKDSYSTKVIANSTKLETGQTVRTPVIPHLQCSPRPLPLRATAMLKILIPKLLDKQGGQLLAVALLIFSRTVISDRIASLNGTTVKFVLEQDKAAFVRLVGVSVLQSAANSFVAPSLRTLTSRLALGWRIRLTNHLLQYYLRRNAFYKVFNMSGKSIDADQRLTLDVDKLTTDLAGLVTGMVKPLVDILWFTWRMKLLSGRRGVGILYAYMLLGLGFLRAISPDFGHLASEEQELEGTFRFMHSRLRTHAESIAFFGGGSREKAMVEAKFMKLLNHSKVLLRKQWLYGVVDDFVTKQLPHNVTWGLSLLYALEHKGDRALTSTQGELAHALRFLASVVSQSFIAFGDILELHKKFLELSGGINRIFELEEILRVAQKDTPVPSSAISAASDEIIEFHEVDIVTPSQKLLARKLSCSVVQGKSLLLTGPNGTGKSSVFRVLRDLWPAFSGRVTKPSEGMFHVPQSPYTSLGTLRDQIIYPLSREEAEMKILSLYKSSNRSSAPELLDDHLKTVLVNVRLVYLLEREGWDSTPNWEDVLSLGEQQRLGMARLFFHHPKFGILDECTNATSVDVEEHLYRLATDMGITVITSSQRPALIPFHSVELKLIDGEGNWELCEIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMI9</accession>
    <name>A0A8I6WMI9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMI9"/>
    <dbReference type="SMR" id="A0A8I6WMI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0097430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="47"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25169" checksum="36AF48C8FF9FE2FF" modified="2022-05-25" version="1">MPSRFLKVLAHGTTKLDVVYTNESREVPFFLEQLKEKWFDDAMEHEKFLGLDLEYTADQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSGITFATVDIRNEKLKMRHNFGIEIPAGCLVDLQTIFRLRHDRTSMAHMVVALIDEEYGDMKTSFPKSQHKLWEKAPLDPINIEYATKDAYVSYELYRKIPVVNYGQRHLEEGGHSVSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMS9</accession>
    <name>A0A8I6WMS9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Patatin</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123425863</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="function">
        <text evidence="7">Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Hydrolyzes phospholipids as well as galactolipids. May play a role in disease resistance.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the patatin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_044965542.1">
        <property type="nucleotide sequence ID" value="XM_045109607.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WMS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0140060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0140060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0140060"/>
    </dbReference>
    <dbReference type="GeneID" id="123425863"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0115430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0115430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0115430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0140060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0140060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0140060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425863"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07211">
        <property type="entry name" value="Pat_PNPLA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045217">
        <property type="entry name" value="PNPLA8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PNPLA" evidence="11">
        <location>
            <begin position="545"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="21"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="8">
        <location>
            <begin position="549"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="8">
        <location>
            <begin position="581"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="8">
        <location>
            <begin position="774"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="583"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0140060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1332" mass="146755" checksum="2A0176BC03CEA43B" modified="2022-05-25" version="1">MSSWGLGWKRSSEIFHLTLDYGDFADEPDQDPSSPPAPPPQSPTAASPTASSSPVATMNGDLGFRIELDWSTSDDEDQVALRLQSQLMVALPPPHDVVCVDLKPADDGDEVGVEMRVVRRREALRSVRVARAMGSTQSTGDGAVVLARLIRSNLAPAPAADGAVAAGVPVLADHWRSVAVLSLCNCGLMVLPVELTRLSFLEKLYIDNNKLSVLPPEVGDLKNLKVLTVDNNMLVSVPVELRQCVLLEELSLEHNKLVRPLLDFRSVPKLRVLRLFGNPLEFLPEILPLHNLRHLTLANIRIDALESLKSVTVEIETENYSYFIAARHKLSAFFSLVFRFSSCHHPLLASALAKIMEDRSNQVAISKEENAVRQLISMISSDNRHVVEQACLALSSLASDISSAMQLIKCDIMKPIEAVLKSFDDEELISVLQVVVTLTFVSDHVAQKMLRKDVLKSLKALCAHKNSEVQRLSLFAVGNLAFCLETRRTLIHSESLRDLLIRSTFSQEKRVSKAAARALAILGENENLRRAIRGRPVAKKGLRILSMDGGGMKGLATVQMLKQIEQGTGKRIHEMFDLICGTSTGGMLAMALGIKQMSLDQCEEIYTKLGKLVFAEPVPKDESATWKEKLDQLFKSSSQSFRVVVHGSKHSADQFERLLKEMCADDEGDLLIESSVKGIPKVFAVSTLVSAMPAQPYIFRNYQYPPGTLEVSPGMAESPSTGAVGTVVSGAPVGIKRGAFMGSCKHHVWEAIRASSAAPYYLDDFSDDVNRWQDGAIVANNPTIFAIREAQLLWPDTRIDCLVSIGCGSVPTKSRRGGWRYLDTGQVLIESACSVERVEETLDTLIPMLPEMQYFRFNPVDDRCGMELDETDPAVWLKLEAATEEYIQKNLQVFKNVCELLVPRYQEEEKSSGIIKSLSFSRLSSSKSGLSESNPTLGWRRVVLLVEASFSPDFGKKINHTRSLEAFCSQNGIRLTLMNSTSGFGNPTTALPTPITSPLFTGSFPSSPLLYSPEGTQRIGRIDLVPPLSLDGHPLMKSSPPTSPIKSWQPSGHVRSLYDKLQNMPQVGVIHLALQNDSTGSILSWQNDVFVVAEPGELADRFLQCVKTSLSTMLHGCKRKGAYSVSKISCLSELVAEWPSFEIGGIHHRYIGRQTQVMEDNQEIGAYMFRRTVPACHMSPEDVRWMVGAWRERIIVCSGKYGLVHGLVKAFVDSGAKAVISSSVEPPDSQAIAYHGMDVSGSLENGKFVIGDDEADESEPEPVSPISDWEDSDAERNGEGNKDIDEEEYLAQFICLLYDKLFREGVTVDTALQQALRAHPRLKYSCHLPNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WP87</accession>
    <name>A0A8I6WP87_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acidic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WP87"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035291232" description="Acidic protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11792" checksum="EC67CA7F9883B136" modified="2022-05-25" version="1">MAKSWQMFVTLMSLLLLCTGLRVHGGEDANIKTSSIPVQPARRVLPATYFPQTTVSRQCDPCGCSHDNDKTFYDTICCNEITCNDPAHPSGTCTVQKVSCGCDVKTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQ22</accession>
    <name>A0A8I6WQ22_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B-keto acyl reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ22"/>
    <dbReference type="SMR" id="A0A8I6WQ22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0145970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0145970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0145970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0176510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000341">
        <property type="entry name" value="very-long-chain 3-oxoacyl-CoA reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF22">
        <property type="entry name" value="B-KETO ACYL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="31610" checksum="B72D182F096D3376" modified="2022-05-25" version="1">MWLYAAFLRPAKPLRRRYGAWAVITGPTDGIGRALAFELAAAGLGVVLVGRSPDKLAAVSKELRARHPGAGVRTFVLDFAADGLAANVATLAESIRGLDVGVLVNNAGHCYPYARYFHEVDEELTRNLVRLNVEALTRMTHAVLPGMVQRKRGAVVNIGSGAATMLPSDPLYAVYAATKAYVDQFSRSLYVEYRNKGIDVQCQAPWYVATKMASIRHASLFAPSPETYARAAVRYIGYEPRCTPYWAHALVWFLITIVPEPIVDKYVLGVSLGIRDKGRAKEARKKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WU80</accession>
    <name>A0A8I6WU80_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WU80"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0058350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0058350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0058350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0046220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0046220.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0046220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0058350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0058350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0058350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33622">
        <property type="entry name" value="OS03G0724500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33622:SF1">
        <property type="entry name" value="OS10G0557800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0058350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="10339" checksum="D128284FEEBE9943" modified="2022-05-25" version="1">MSGGVQSSVPQPSSSSSPVKPASTSCRKKNSGNTSFVTQLRDHFHEFVHASMDEHRTCLTNTVKKLYAMSKAAAERTAGAKEAGAESVLPLESEVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WVZ7</accession>
    <name>A0A8I6WVZ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Dicer-like protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May interact with ARGONAUTE1 or PINHEAD through their common PAZ domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <dbReference type="SMR" id="A0A8I6WVZ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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            <end position="215"/>
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            <begin position="512"/>
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            <begin position="851"/>
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            <begin position="1212"/>
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        </location>
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        </source>
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        </source>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="1299" mass="147434" checksum="12B190BD74CB5E45" modified="2022-05-25" version="1">MNNNSTRFKHHYLNKAISVLNCNILDGTHDDSFNLEMLKEPFFSNKIVVLINILSRYRLEEKMKCIVFVKRITVARAIADILQNLKGLDLWKCEFLVGRHSGPKNMSRQKMDAIVENFSSGEVNLLIATSVGEEGLDIQTCCLVVRFDLPETVASFIQSRGRARMTNSKYVVLLERGNHSQEKLLNDYIDGEGIMNGEIDLRTSNDVFDHLEEKSYRVEKTGASISTACSVSLLHRYCYNLPKDMFFNPSPAFIYIDDTEGIICRVILPPNAAFRQVDGQPCQSNDEAKRDACLEAYVKLYELGALTDFLLPGSGSRKNSASTINGSANNSHDDESLREELHEMLIPTILKPSTCKLDCPLNLHFYYIQFFPIPADRHYRIFGLFVINPLPMEAEKLEVDLHLARGRIVKAGMKHLGTISFDEEQMMLGRNFQEMFLKVLLDRSEFTVSHVMLGNNETLLFNSTFYLLLPIKQELYGDIFMIDWPTVKRCLSSPVFKDPTGVSVHGSYLPDESLRLLDNMYSKTDVVGSLIFAPHINTFFVIDVILNELNARSEYDGATYEDHYKERFGIKLSHPEQPLLHAKQLCNLHNLLHDRLRETTGRELMEHFVELPPELCSLKIIGFSKDMCSSLSLLPSLMCRLENLLVAIELKDVMLSSFSEASQISASGILEALTTERCLERISLERFEVLGDAFLKYVVGRHNFLTYEELDEGQLTSRRSAIVNNSHLYELSIKRNLQVYIRDQHFEPTQFIALGRPCKVVCNADTEVNIHTDSRENCNLRCTKSHHWLHRKTIADAVESLVGAFLVEGGFKAAFAFLFWVGIDVDYKDSSLYRVLDASSINLSLTSHTNVDELEELIGYNFKHKGLILEAFVHPSFNKHSGGCYQKLEFLGDAVLEYLITSYLYSAYPDLKPGQITDLKSLAVSNNSLAYVAVQKGIHKYLIKDSNYLSTAVNKFENYIRLPNSEKDLVEEPACPKVLGDIVESCVAAVLLDSGFNLKCVWTLVLMLLEPVLSFSDMHMNPMREIRELCQCHELELGLPKPMKADGEYHVKVEVNINSQVISSAAANRNSKVARKFAAQETLSKLKNYGYTHKNKSLEEILRDARKKEPELLGYNEEPIKVEADVSAEINNLKIGKERDANISFQNTEIFIRGTLRTSSQRTAGDTMFSKDDVSIERNNQLKVAKQNGCLPKGTTQKNIKKEYHGHMVNKTAKSFLFEVCAVSYWKPPEFELCKEEGPSHLRRFTYKVTVQISGPSETLLECYSDAQLQKKAAQEHAAQGALWYLKQHGYLPKDEVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WY22</accession>
    <name>A0A8I6WY22_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WY22"/>
    <dbReference type="SMR" id="A0A8I6WY22"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0088450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088450"/>
    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="142" mass="16550" checksum="D2FC6D2B53F635D9" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDLEVCEPEIPTHIEILLEKLNLVEKENKYLKEKLKIIEDKNIKLELHVVDVIDDRKMKIDAMLLKMDAMRLKMDAMRLKMDEMCLKIRNIRKYAIDKEAWYHYAVGPIVTLVVILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WY91</accession>
    <name>A0A8I6WY91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123430956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970718.1">
        <property type="nucleotide sequence ID" value="XM_045114783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WY91"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0201580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0201580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0201580"/>
    </dbReference>
    <dbReference type="GeneID" id="123430956"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0167450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0167450.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0167450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0201580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0201580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0201580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430956"/>
    <dbReference type="OrthoDB" id="1624361at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF438">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0201580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15768" checksum="57D804485D90486E" modified="2022-05-25" version="1">MAKCSKIRDIVWLRQTLRRWRSRAAARVAEGGAASAAVPAGHVVVCVGGASRRFVVRAAHLNHPVFLELLRQAEEEYGFRTGACGPVALPCCDEDRFRDVLRRVSSEERRCRLFGCRVPAASARDVATRPLLQRAAAEELVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZ78</accession>
    <name>A0A8I6WZ78_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibronectin type III-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123428208</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044968328.1">
        <property type="nucleotide sequence ID" value="XM_045112393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZ78"/>
    <dbReference type="SMR" id="A0A8I6WZ78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206500.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206500.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206500"/>
    </dbReference>
    <dbReference type="GeneID" id="123428208"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0171570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0171570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0171570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0206500.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206500.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428208"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009044">
        <property type="term" value="F:xylan 1,4-beta-xylosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001384">
        <property type="entry name" value="Beta-D-xylosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000001">
        <property type="entry name" value="probable beta-D-xylosidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000004">
        <property type="entry name" value="probable beta-D-xylosidase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044993">
        <property type="entry name" value="BXL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721:SF14">
        <property type="entry name" value="BETA-D-XYLOSIDASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="SUGAR HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="8">
        <location>
            <begin position="792"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206500.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="868" mass="91887" checksum="F6C484225E1710BB" modified="2022-05-25" version="1">MAPRRASVTGWVEYKPQIARRVEAGAKRRRKQSLATAVPVTGRSSSRPRPRHALAQPPPLYISPVPVPVPRRRGGESQSTHARTLASQPAAMATAARPPFLAMAAAALLVAAWWGGNAGAAEAQAQAPVFACDASNATLAAYGFCNRKASASARARDLVSRLTLAEKVGFLVNKQPALGRLGIPAYEWWSEALHGVSYVGPGTRFSPLVPGATSFPQPILTAASFNASLFRAIGEVVSTEARAMHNVGLAGLTFWSPNINIFRDPRWGRGQETPGEDPLLASKYAVGYVTGLQDAGAGGVTDGALKVAACCKHYTAYDVDNWKGVERYTFDAKVSQQDLDDTFQPPFKSCVLDGNVASVMCSYNKVNGKPTCADKDLLEGVIRGDWKLNGYIVSDCDSVDVLYTQQHYTKTPEEAAAITIKSGLDLNCGNFLAQHTVAAVQAGELSEEDVDRAITNNFIMLMRLGFFDGDPRQLAFGSLGPKDVCTSSNRELARETARQGIVLLKNSGALPLSAKSIKSMAVIGPNANASFTMIGNYEGTPCKYTTPLQGLGAKVNTVYQPGCTNVGCSGNSLQLSTAVAAAASADVTVLVVGADQSIERESLDRTSLLLPGQQTQLVSAVANASSGPVILVVMSGGPFDISFAKASDKIAAILWVGYPGEAGGAALADILFGSHNPSGRLPVTWYPASYADTVTMTDMRMRPDTSTGYPGRTYRFYTGDTVFAFGDGLSYTKMSHSLVSAPPSYVSMRLAEDHPCRAEECASVEAAGDHCDDLAFDVKLQVRNAGEVAGAHSVLLFSSPPSAHNAPAKHLLGFEKVSLAPGEAGTVAFRVDVCRDLSVVDELGGRKVALGGHTLHVGDLKHTVELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4A4</accession>
    <name>A0A8I6X4A4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4A4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0168200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0168200.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0168200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0168200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0168200.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0168200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0168200.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26446" checksum="E03DA60F37643989" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTMEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQTTVEMLENRDKDARALHMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDLQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8N4</accession>
    <name>A0A8I6X8N4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein GOS2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein eIF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123444606</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977322.1">
        <property type="nucleotide sequence ID" value="XM_045121387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8N4"/>
    <dbReference type="SMR" id="A0A8I6X8N4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294710"/>
    </dbReference>
    <dbReference type="GeneID" id="123444606"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0245250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0245250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0245250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0294710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444606"/>
    <dbReference type="OMA" id="MYHAGQE"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="10">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="12641" checksum="7806BA6716C832F8" modified="2022-05-25" version="1">MSDLDVQVPTAFDPFAEANAEDSGAGAGSKNYVHVRVQQRNGRKSLTTVQGLKKDYSYNKILKDLKKEFCCNGTVVQDPELGQVIQLQGDQRKNVATFLVQAGLAKKESIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XA82</accession>
    <name>A0A8I6XA82_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123451191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984135.1">
        <property type="nucleotide sequence ID" value="XM_045128200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XA82"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345230"/>
    </dbReference>
    <dbReference type="GeneID" id="123451191"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0288190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0288190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0288190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451191"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035242883" description="TLDc domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="73"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345230.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="273" mass="29526" checksum="14E827FDE93515E6" modified="2022-05-25" version="1">MPTRIQLMLTSLISGLLLLHSYPSSLARAISNTTFMASARVAMVPTRASFVTPRSFFNWGRGAKEAETPPPPQFQYHGVEPPFPMSLVANTHLRGRELSCCYRATVDGFSATDFHRRCDFKGPCVIVGYTAGGSFRFGGFSPEGYRSTDDYYDTLDAFLFYWPPASPEVTTAAVPVVLPKVGGSGAALFDYSRGGPQFGADGLLIGPPLSAVMGVFTGPDASVGVGDLRRARSRLGLSYARREDGKESLFGDESKAELEEVLVFCSPQIASMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEG9</accession>
    <name>A0A8I6XEG9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123441174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973613.1">
        <property type="nucleotide sequence ID" value="XM_045117678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEG9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329080"/>
    </dbReference>
    <dbReference type="GeneID" id="123441174"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0274010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0274010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0274010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0329080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441174"/>
    <dbReference type="OMA" id="RIGIANE"/>
    <dbReference type="OrthoDB" id="586540at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF48">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="47"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="420" mass="46941" checksum="A7B969789DBC5C68" modified="2022-05-25" version="1">MEMLGGGRSTSTDASAKPKRPRHAVDDGIGTGTGTDADGSSGGDADSDRLSALPDDLLREILSRLNALETAQTCVLSARWRNLWRSVPCLDIDEREFTAKYPFFVNFTGNLLRGHEVALLEDLRVYLHTGVLGWSAPWVRRAIGLGVGEAWPCRLKRLHLYSVHDLQLTDLGRHISSHCPALEDLKLQNCCYLVTGTFKIASSSLKKLVVVGDHEDDDADGLFYFVIDAPALVSLCLGSSDIYHISATPTDPHSMPSLTDASIRLCPLTTFGIPSTAYESGLLATLYNVTSLHLLHLGVKLWHCEDYLEFPIFENLRTLSLDQCYIAGDFMGLEPYLRNSWNLEKLTLRRCKVLDFRTKMKEEEHLHKYSQDPMDFMCENLTITEIIYQDGDASVDLLVKYLVGMSRNLPNNKIELTKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEU5</accession>
    <name>A0A8I6XEU5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein RABA1f</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976601.1">
        <property type="nucleotide sequence ID" value="XM_045120666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEU5"/>
    <dbReference type="SMR" id="A0A8I6XEU5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0278630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0278630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0278630"/>
    </dbReference>
    <dbReference type="GeneID" id="123444060"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0230860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0230860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0230860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0278630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0278630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0278630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444060"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0278630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24055" checksum="3EEEF0F3FC1DF018" modified="2022-05-25" version="1">MAVAYRAEEEYDYLFKVVLIGDSGVGKSNLLSRFARDEFSLDTRSTIGVEFATKTVRVDGKLVKAQIWDTAGQERYRAITSAYYRGAVGALVVYDVTRHITFENAERWLTELRDHTDANIVVMLVGNKADLRHLRAVSPEEARAFAERHRTFSMETSALEATNVEDAFTEVLGEIYRVVSKKALDIGDDPAAPPRGKTIDVGAKDDVTPVNSGGCCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XF27</accession>
    <name>A0A8I6XF27_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC123451075</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984023.1">
        <property type="nucleotide sequence ID" value="XM_045128088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XF27"/>
    <dbReference type="SMR" id="A0A8I6XF27"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338560"/>
    </dbReference>
    <dbReference type="GeneID" id="123451075"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0282400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0282400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0282400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0338560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451075"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000016">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035309749" evidence="19">
        <location>
            <begin position="28"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="33"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="142"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="366"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="671" mass="73765" checksum="0105818C8D0989D7" modified="2022-05-25" version="1">MATAPRRLLLLLVAVVMSSSCVGVAVAEGQTYWPVKPYCSTTSNYSADSRYRLNLLTLVTDLPARAFDNSFYYARTAGESPDEVFGLIACYADRSWNQCRDCLYAAANGIQQSCPFSRRMKGAYDACLLRYSDESFASVADLSVAFDTSDKTKVDYPASMSDTRWDLLTRLVAEAAHSPLRLANGSVPYAATSQSPTTTMHGMVQCTRDLNASECSRCLTEFVSNLSAMFPNNTGGMMKGYSCYAIYNIGDGDFLRVTLPRPSNISPGEQPPPTNLRPGAGDTPPLAADRTSSRTPLVAGVSAGVVVVFFISSSILVCFLLRCRRRNRMVRQVDTFDDDPLGDEFEKGTGPKRFRYSDLAVATSNFSDEKKLGEGGFGSVYKGFLKELKTEVAIKRVSKTSKQGRKEYISEVKIISRLRHRNLVQLLGWCHGGGQLLLVYDLMPHGSLDTHLYSSNATLSWPLRYEIVLGLGSALVYLHQDWEQCVLHRDIKPSNIMLDASFCAKLGDFGLARLVDHGRGPYTTGLAGTMGYMDPECVVTGRTSAESDVYSFGVVMLEIACGKRPAVSRGREDVIHLVQWVWDSWESGRILDAADAQLNLEFDRREMQCVMVVGLWCAHPDRSLRPSIKQAVSVLRFEAPLPSLPAKMPVATFTTVIDSSLVSASQLSAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGF9</accession>
    <name>A0A8I6XGF9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein ECERIFERUM 26-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123427645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967670.1">
        <property type="nucleotide sequence ID" value="XM_045111735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGF9"/>
    <dbReference type="SMR" id="A0A8I6XGF9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191470"/>
    </dbReference>
    <dbReference type="GeneID" id="123427645"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0158660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0158660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0158660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0191470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427645"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF115">
        <property type="entry name" value="PROTEIN ECERIFERUM 26-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="47398" checksum="40ADA99ED0D18B4B" modified="2022-05-25" version="1">MGLEGEATAAVHGHRLSTVVPSSVTEVEGYELGDADLAFRLHYLRGVYYYAAGEVVRGVTTKVLKDPMFPWLDAYYPVAGRVRRPADDAAPAAAEQQQQRGEASRRPYVKCNDCGVRIVEARCERALDDWLRDGAVDRVRLLCYDKVLGPELFFSPLLYVQVTNFKCGAMALGFSWAHLIGDVATATTCFNHWAKILGGKTPDAVTVNPRNEPQDRAPADAAVPRSVKPVGPIEDYWFVPAGVDMAGYSFHITEPMLTRLQQQQQQEPAAAGGAFELISALLWQTVAKIRAGKEVKTVTVVRNDMSARSGNSLANEQRVGYVEAGSPPAKTDVPELAALLAKDVVDETAAVVAFPGDVVIYGANLTFVDMEQVDLYGLEIKGQRPAHVEYGLDGVGEEGAVLVQPDADGRGRVVTAVLPKDEAEALRAALGSTLLHPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHE5</accession>
    <name>A0A8I6XHE5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123440541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973041.1">
        <property type="nucleotide sequence ID" value="XM_045117106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHE5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302740"/>
    </dbReference>
    <dbReference type="GeneID" id="123440541"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0252580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0252580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0252580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302740"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040344">
        <property type="entry name" value="At3g17950-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33544:SF5">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33544">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="146" mass="15136" checksum="79BBBE4EBA98C841" modified="2022-05-25" version="1">MAHEDGWPLGLGLGTLNPRAGAVRSVGSSSSSTAAFTPSHCVSSVASSDLDTESAWSLPHAGRGGGITLAALIGLVDAMESRRSRRPGERAGRGGKLRALLLSLCLRSHLENGGGAAPSLGQFLEMERRASGSSGHLHRMLPWNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK75</accession>
    <name>A0A8I6XK75_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK75"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0402200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15010" checksum="30DB07EE30353CDF" modified="2022-05-25" version="1">MFVSTSEKHDGWVFYKCQNHGVSCEFWHWELEYVQYLVDYHYLVGDAAVDAIGAAEERREQLLNAQELHYMNGSTSMSKKFVKRESGDVMTRQQAAALMKLGRELVMLMKILLAVVLMLGVGALVLYMVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKB1</accession>
    <name>A0A8I6XKB1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123397144</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947740.1">
        <property type="nucleotide sequence ID" value="XM_045091805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKB1"/>
    <dbReference type="SMR" id="A0A8I6XKB1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510260"/>
    </dbReference>
    <dbReference type="GeneID" id="123397144"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0510260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397144"/>
    <dbReference type="OMA" id="MSDRGGP"/>
    <dbReference type="OrthoDB" id="1932364at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045277">
        <property type="entry name" value="DRE1A-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31839:SF85">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31839">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="12"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="190" mass="19754" checksum="62A232B7A00B9205" modified="2022-05-25" version="1">MSGHASSGKHPFYRGIRSRHGKWVSEIREPRKARRIWLGTFPTAEMAAVAYDVAAHALRGAGAALNFPALAATRPAPAFTSADDIRTAAATAAASMQHDRAIGGIAPAAAGSGGSSAAAASASGTVQQDQAGIGLDRGFLDEEALFETPQFLRNMAAGMMMSPPRLSPDSSDESPDPSEAGESLWSYRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLC0</accession>
    <name>A0A8I6XLC0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ABC transporter B family member 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123448984</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(indol-3-yl)acetate(in) + ATP + H2O = (indol-3-yl)acetate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:84235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:84236"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981978.1">
        <property type="nucleotide sequence ID" value="XM_045126043.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6XLC0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386920"/>
    </dbReference>
    <dbReference type="GeneID" id="123448984"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0321840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0321840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0321840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0386920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448984"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM43</accession>
    <name>A0A8I6XM43_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC123450062</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">subsp. vulgare</strain>
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        <property type="project" value="UniProtKB-KW"/>
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            <end position="186"/>
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        </location>
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        <source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418400.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="427" mass="47725" checksum="C009536D80BD8BDC" modified="2022-05-25" version="1">MFTVSHSTSLPYRPRHRSRRRAPSEEVSDAEMSYGEVPPRQDAESLIRGAANRAAPGSAGEEDEEMYDDDRSSDASDYSYDDDDDSGESGWYYEDREEEEEQEGAGGDRACGSAEEEAASAGVGGGPKAEGSAQAAATCCICMDPWAPYGAHRICCISCGHVYGRSCLERWLLRCGNVSAKCPQCSERFKHRHIINLYSPVHLWNDCCLKEIRAQSTSSISSVHPCPDAISFRISQLEARMEEERRLRDEAVRRQEERIAAQNRFFEERTAAQNQLFEERMAEQSQFFKEQRAAQRQDFEESRAAQRQDFEESRAAQYKYFEERLAAQTLAFKEMLAAQSQAIKERISRGIEERLEAERALYQEQFQSPDALNGKSPLLMPSLPPPPAPHTHTHLLAGSNDPGGGTCAHDQGSLSTHHGGATPTSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMJ8</accession>
    <name>A0A8I6XMJ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123453110</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044985810.1">
        <property type="nucleotide sequence ID" value="XM_045129875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMJ8"/>
    <dbReference type="SMR" id="A0A8I6XMJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527630"/>
    </dbReference>
    <dbReference type="GeneID" id="123453110"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0438870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0438870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0438870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0527630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123453110"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="55"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="490" mass="53870" checksum="717B7EA4551076AA" modified="2022-05-25" version="1">MGARCSKLSACWWPPHFKSPRLENGAAGEDGSGVPVFAEYSLDELRAATDGFATDRIVSEHGEKAPNVVYRGTLFSSGATVAIKRFGRSAWPDARQFVEEARAVGLLRSGRLSNLIGCCCEGGERLLVAEFMPHDTLAKHLFHWETKPLSWAMRVRAALYVAQALEYCTLKGRALYHDLHAYRVLFDVDGNPRLSCFGLMKNSRDGKSYSTNLAFTPPEYLKTGRVIPESVVYSFGTILLDLLSGKHIPPSHALDLIRGRNITVLMDSCLEGHVSSSDGTEMVRLASRCLQYEARDRPNLKAVVSALASLQKDASAPSHTLLGISQDAVKENAEQVSFSATEKAYATANLEQVHELLENEGYDEDETASFKVSLSSWPGQPSESIQVKKNGDDAFQSKDFTTVLECYARFIDTGAMESPTMLVRRGFANVVLGRMEDALEDARRAEGISPEWPTAHYLQGMALIGLGMELDGHEKLRIAASLEAQRTGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMZ8</accession>
    <name>A0A8I6XMZ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxyproline-rich glycoprotein family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949765.1">
        <property type="nucleotide sequence ID" value="XM_045093830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMZ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530690"/>
    </dbReference>
    <dbReference type="GeneID" id="123399418"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0441440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399418"/>
    <dbReference type="OMA" id="DYFQDPA"/>
    <dbReference type="OrthoDB" id="692030at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37256">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37256:SF1">
        <property type="entry name" value="MYB-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40816" checksum="669D4F9D2BAD4FB8" modified="2022-05-25" version="1">MRNKRLAPTTCSSSKTNSAKEEEPHLSGAYIRSLVKYLSSSSTARSKDHHHITMGTKPQQEEQQAPQTTPPSLQQQQPHKKQVRRRLHTSRPYQERLLNMAEARREIVTALKIHRASMKEAKEQQQHQQLVQQLQHQQEVQVVQDHRVVCSAPTMSSYGSFSDFPFAHSPATNSSCSYYSSPLLPYHTPAAPPMVPMVDALDHLLPLPTQPLGLNLSFHGFGGGVAGEDAKNNTASSFFDPPPLLQQPSPASSYSVYSSPPGTTMASQDVASATAENTSPSLHQVLDEEEMAAIYSVGERHDIEWSDTLNLATSVWWSTLLDDGAAAAHQSDSVDVPGMHLGDVYYGEDVSFPRMEIGEMEGWDEEWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNS9</accession>
    <name>A0A8I6XNS9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Sucrose synthase</fullName>
            <ecNumber evidence="3 7">2.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123427512</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-diphosphate + sucrose + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:76533"/>
            <dbReference type="EC" id="2.4.1.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase 1 family. Plant sucrose synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.13" evidence="3 7"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNS9"/>
    <dbReference type="SMR" id="A0A8I6XNS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0155790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0155790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0155790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0188120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016157">
        <property type="term" value="F:sucrose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1230:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.330:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000006">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012820">
        <property type="entry name" value="Sucrose_synthase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056736">
        <property type="entry name" value="SUS_EPBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056735">
        <property type="entry name" value="SUS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02470">
        <property type="entry name" value="sucr_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839:SF4">
        <property type="entry name" value="SUCROSE SYNTHASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24862">
        <property type="entry name" value="SUS_EPBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24861">
        <property type="entry name" value="SUS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase N-terminal" evidence="11">
        <location>
            <begin position="57"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase EPBD" evidence="12">
        <location>
            <begin position="207"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="10">
        <location>
            <begin position="317"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="9">
        <location>
            <begin position="619"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="858"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU280817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="911" mass="104004" checksum="DFEE9AA65080E484" modified="2022-05-25" version="1">MCVPSPPVLDAGFVSRPLPINRCPSTPRQITTAQPRRSSLVSFPSLAMASAKLSFKRMDSVAESMPDALRQSRYQMKRCFQRYVSRGRRLLKNQQLMEELDRSLDDELEKEKLVEGFLGYIICSTQEAVVLPPFVAFAVRMNPGIWEYVKVHADDLSVEGITPSEYLKFKDTLYDEKWAKDDNSLEVDFGALDLSTPRLTLPSSIGNGMQFVSKFMSSKLNGKPESMKPLLDYLLALNYRGEKLMVNDTIDTVNKLQTALLLAEVFVSGLPKFTPYLKFEQRFQEWGLEKGWGENAERCKETLNFLSEVLQAPDPINMEKFFSRVPSIFNIVVFSIHGYFGQEKVLGLPDTGGQVVYILDQVRSMEEELLQRIKLQGLHITPKILVLTRLIPDSKGTKCNVELEPVENTKYSHILRVPFKTEDGKDLRQWVSRFDIYPYLERYAQDASTKILDMLEGKPDLIIGNYTDGNLVASLMSSKLGVTQGTIAHALEKTKYEDSDVKWRELDQKYHFSCQFTADMFAMNTTDFIITSTYQEIAGSKEKPGQYEHHYAFTMPGLCRFATGINVFDPKFNIAAPGADQSVYFPFTQKQKRLTNLHPQIEELLYSKEDTDEHIGYLADRSKPIIFSMARLDKVKNITGLVEWYGQNKKVRDLVNLVVVAGLLNAAQSKDREEIDEINKMHNLIDKYQLKGQIRWIKAQTDRVRNGELYRYIADSKGAFVQPALYEAFGLTVIEAMNCGLPTFATNQGGPAEIIVDGVSGFHINPMNGREAGTKIADFFQKCKEDPSYWNKMSTAGLQRIYECYTWKIYATKVLNMGSMYGFWRTLNKEERVAKQRYMQMFYNLQYRNLVKTVPRVGEQPPRPAASTGAVAERNQIVARPRERKPQGRVQRMMTSLLGPKPPTYEQNGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XP22</accession>
    <name>A0A8I6XP22_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="3">6.3.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formyltetrahydrofolate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123398418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044948827.1">
        <property type="nucleotide sequence ID" value="XM_045092892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XP22"/>
    <dbReference type="SMR" id="A0A8I6XP22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485330"/>
    </dbReference>
    <dbReference type="GeneID" id="123398418"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0401870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0401870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0401870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0485330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398418"/>
    <dbReference type="OMA" id="REDHFVI"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1510.10:FF:000003">
        <property type="entry name" value="Formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1510.10">
        <property type="entry name" value="Domain 2, N(10)-formyltetrahydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="721" mass="76853" checksum="D31FBD0C7917D5E6" modified="2022-05-25" version="1">MDLPAPPTNHPRTLSSIILSRRRPDAGARIYNCSATASQRRLLLSPPPPPRHHSLPPRLRPDPPARAVARSSAVSAPPLVPPMTTPAPNPTIRRLDVASPVPADIDIANAVEPLHIADIAAELGVPPEHYDLYGKYKAKVLLSVLDKLQGQQDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLDKKVVTCVRQPSQGPTFGIKGGAAGGGYSQVIPMDEFNLHMTGDIHAITAANNLLAAAIDTRIFHEASQSDKALFNRLCPPNKEGKRRFADVMLRRLIKLGISKTDPDELTPEEVRRFARLDIDPASITWRRVMDVNDRFLRKITVGQGPEEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGRMVIGNSKAGEPVTADDLGLGGALTVLMKDAIHPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGKGGFVVTEAGFGADIGTEKFMDIKCRYSGLTPQCAIIVATIRALKMHGGGPDVVAGKPLDHAYVSENVALVEAGCVNLAKHISNTKGYGVNVVVAINKFATDTDAEMEVVKNAAMAAGAFDAVVCSHHAHGGKGAVDLGLAVQRACESQAEPLKFLYPLDSSIKEKIESIAKFYGASGVEYSEQAEKQIEMYTKQGFSNLPICMAKTQYSFSHVPSMKGAPSGFVLPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDIDTATGKVMGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQA9</accession>
    <name>A0A8I6XQA9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123430906</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970671.1">
        <property type="nucleotide sequence ID" value="XM_045114736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQA9"/>
    <dbReference type="SMR" id="A0A8I6XQA9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0199150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0199150"/>
    </dbReference>
    <dbReference type="GeneID" id="123430906"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0165440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0165440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0165440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0199150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0199150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0199150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430906"/>
    <dbReference type="OMA" id="QEHEVTH"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF889">
        <property type="entry name" value="OS03G0379801 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="193"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="428"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="594"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="907" mass="104086" checksum="DF9181B4E095AA38" modified="2022-05-25" version="1">MADALIGPLVGRLQELALSQARALVAVNKDIRRLRDKLMFLQAFLREADVKRHLFSDEITRVWLQQTRDAVFDAEDAVDHYYLQVDMSRYPWWARPSMRYVATFTTQVTMRRKLSVKVKDINSRLEDIIENKDKYKMEDMNKKSELTWKAATSISYAHRKLDDLVPPHMPIHHDKRKKLENVLLHPIEEENEYHHHPVVISVVGKSGVGKTTLVRDVYDKIDKKRFTVHAMESFAPYLTAINIQWQIVQKLSGDGKNCTRSNVDKVFEEKTIGQKYLLVIDGEVSSTDWKNILLSLKISAKGSRIVHITQHKPEEPPNGYPHVIINLESLGQKTAVELFHRRLLHIEVQDKKIHDRHQDIHNITEGLPLGIALLSGLLQTKEFPSEWTNVFDYLMSKQSKRLHRILSVCFDDLPHELKCCFLYFAALPANIKIEARRLVCLWMAEGFLRPKGGKTMEKVGYIYLNELIARNLVKPVHMDDEDTSSAGSIFVTIQNKIHEFLQFEAQEASFVEVHNGDDIPTLTSARRLSLQNSTDKYAVLASSLPKLRSIFSHFEQDYKDPKEDQIYLCCSPQLRKISKWKRNKDIKSHIQGLLHRSEFLRVINLQGIEIGQRLPDEIGNVVHVHYLGITSCSLAEIPQSIGRLTSLQTLDVRETNVRELPLAFWMIKTLRHVFGGFLKFPEKIGGLKNLQTLDSVQLDTLEQDLVGTLGEMIHLEYLRVGYSSEGDVGALSDALNKLENLRTLIIKGKNIPSSVFTSYTHRRLKFIVLEGKLDLPSELNGGFFLPNLITLSLVRTSISQEFIDKLAELPFLASLALYPNSYRGDCIAISPGGFRCLKKLMVDVEKLKRFEAKNALPKLEELDIHSQYGEYCCLFKENVDQKKTIVADLLKKDNVENKRSLIRRLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS54</accession>
    <name>A0A8I6XS54_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444830</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044977621.1">
        <property type="nucleotide sequence ID" value="XM_045121686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS54"/>
    <dbReference type="SMR" id="A0A8I6XS54"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300600"/>
    </dbReference>
    <dbReference type="GeneID" id="123444830"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0250730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0250730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0250730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0300600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444830"/>
    <dbReference type="OMA" id="SSWNTWS"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF58">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035320274" description="chitinase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="27"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="306" mass="31825" checksum="4B4450A202EA99A0" modified="2022-05-25" version="1">MASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAFLFTFGRGQKPLLNLAGHCDPATSGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRPLGDAVLDGVDFDIESGGSAHWDDLARELKKYSGKESAYKPVYLAAAPQCPFPDAMLGGLGGALGTGLFDYVWVQFYNNPPCQYTKAGGAGNLASAWQKWASIPARQVFLGLPAAPAAAGSGFVEPSDLLSEVLPVVQKSPKYGGIMLWSRFFDGQTGFSDKVKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSI1</accession>
    <name>A0A8I6XSI1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSI1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0502010.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0502010"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0502010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0502010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23261" checksum="9F3BFCBC868A4D7F" modified="2022-05-25" version="1">MSLPYSDQSIRPKKMQSASLPRGVEAVRCWCGDLCKVKEVTDFSDWLGMKFFMCANYESYPPESISAYLRPPSPPPLFMYYRWIDTEMPDWAVTEIRERGRRAGASLDLEERREKAEAEEKAAQKKEWEEYCVEQRAFIDEMKRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEAGDAKGKYPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSM3</accession>
    <name>A0A8I6XSM3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123431319</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971076.1">
        <property type="nucleotide sequence ID" value="XM_045115141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSM3"/>
    <dbReference type="SMR" id="A0A8I6XSM3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215740"/>
    </dbReference>
    <dbReference type="GeneID" id="123431319"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0179320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0179320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0179320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0215740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431319"/>
    <dbReference type="OMA" id="EGFMMER"/>
    <dbReference type="OrthoDB" id="592624at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF280">
        <property type="entry name" value="HYDROXYCINNAMOYLTRANSFERASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="440" mass="47390" checksum="CFE7C31C8EC6A709" modified="2022-05-25" version="1">MEAASSTMLKPVYSTPHPVAGEMVPLTLFDRATLDIFVPIILVYAAPTPPNEALKKGLRRAVAVYPHLAGRFAVDHRGRRCIHVNNEGVLVVEAAVPVDLASVVTDGSFVTDTDELYPAVPPPEEIVGAALLQIKLNRYKCGGLVIGFSSHHQAADGYSVSTFLSTWASAVRQGRNFTAPSPFLDRAATAVARTVPTPVFDHRSAEFKGQGGRSYRVAPDPMSEIKNVTVRFTAEFIAELKARVGIRCSTFNCLLAHAWKKMTAARGLKPDEFTKVRVAVNCRARASPPVPADFFGNMVLWAFPRLQAGDVLGWSYRGVVEAIRDAVARVDAEYIQSFVDFGSVADAAGEELAATAAVNGTMFSPDLEVDSSLGFRFNQIDFGAGPPSAFVTPDLPNEGLMIFLPSCTASGGVDLVMAIPEDHDTATFYSLADERAHSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXD8</accession>
    <name>A0A8I6XXD8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </evidence>
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    </evidence>
    <sequence length="135" mass="15778" checksum="C2CD6FBE45DE1415" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDVEVCEPEIPTHIEILVEKLNLVEKENKYLKEKMKIIEEKNMELELHVADVIDDCKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYDIDKEAWYHYAVGSIDTLVAILIAFVLAFKCFT</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </evidence>
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    <sequence length="257" mass="28150" checksum="35515CEA825D1FB5" modified="2022-05-25" version="1">MVSYGEIIVNSMQIVPSSISISGKKFLSYLDACMGEPVVTEMDPSDISGNQKIQSYLVQSPDNSGEKVEVIPTPPVREEMQVRCSKRNMGGNVEHMGVRAESLAQKRDLQGNSISPGNSFEVLSNLEIISAVVKMGVNIPDDDFESIDIIRELEISRANIAKKVDKNDNQHDTVLLITNVAGEASPLDTEWGGDEGLDADDFTVVRSRKKERKNVNITISKHVTRSQKQKVGDGAGKAMAPGKPSTKNHNTRNRKND</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A8I6XYF1</accession>
    <name>A0A8I6XYF1_HORVV</name>
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        <recommendedName>
            <fullName evidence="17">E3 SUMO-protein ligase SIZ1</fullName>
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        <name evidence="15" type="primary">LOC123448150</name>
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        <lineage>
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    <reference evidence="16" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
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        <text evidence="2">Protein modification; protein sumoylation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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    <dbReference type="GO" id="GO:0000785">
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SP-RING_Siz-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 SUMO-protein ligase SIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031141">
        <property type="entry name" value="SIZ1/2_SP-RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF70">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE SIZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="13">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="14">
        <location>
            <begin position="357"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="523"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="701"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="568"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0355130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="778" mass="85244" checksum="70D6FAB84D6C90E9" modified="2022-05-25" version="1">MSSAPAGDPVLAACKDKLKHFRLKELKDVVYKIGLPKHGKKQELVDKIVAILSDQQDQASKIDGLPNKLMVGKETVVKIVEDTFRKMREPANAVSASGNQNELGYSVMPMKNTDVSAQLDIKVRCPCGNSMATGSMIQCDHPRCNVWQHIDCVIMPEKTADTAPQELPSSFYCEMCRVSRADPFWVTINHPLLPVSIAPSNMMADGSYTVQYIEKTFPLSRANREMLQKAEYDIQVWCILLDDKVPFRMHWPLHSDMQINGIPVRVVSRQATQPLGANGRDDGLMLTQFLKEGPNKIVLSRSDSRTFCLGVRIAKRRSLEEVLNLVPKEQDGEKFDDALSRVRRCVGGGAEADNADSDSDIEVVADSVSVNLRCPMTGSTIKVAGRFKPCVHMGCFDLEAFVELNQRSRKWQCPICLKNYSLENLIIDPYFNRITSMIKSCGDDISEIDVKPDGSWRAKGGAEPNDLMQWHLPDGTLCISIGTGPKPNTGVVKPEIREEPLPEYKGSRLKLGIRRNNNGKWEISKKGDVNLKPTSYNDQSRDLENGKCVTHTSNTNHEDAKGGGYNSEPGQSDHPTSSVYDLNSSPGDEHVPIVLSDSDDENATVLSPSAVNCGSANDTGYEFPPPNPLDTSGGPDETSFFLNESFDDLGLSFWEYPSTTQDDPGIQGTDNLGEVQNYPGTNLSLHEPVSTANLDVLAPAPNQLGYGHDGSLNNVKNTSRKRKNPVDEITSLDASVPMSGNDDDDDFVGDGSGGTSSLSGQARSVRTKSVLAIESDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZM6</accession>
    <name>A0A8I6XZM6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123403959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044953798.1">
        <property type="nucleotide sequence ID" value="XM_045097863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZM6"/>
    <dbReference type="SMR" id="A0A8I6XZM6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561650"/>
    </dbReference>
    <dbReference type="GeneID" id="123403959"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0466410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0466410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0466410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0561650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403959"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF48">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="6">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="370" mass="40961" checksum="ED6CA07F946D3A77" modified="2022-05-25" version="1">MSSSSFSFWGVELRPGEPYVHRHPPSLGRLCITKAVLVNNNNNNKTAERAMVQCVIGNGAPVIICSLNPGFVEMCHLDLEYEENHEVHFSVLGAASVHLSGYYLRGCNCLIRYPEVAPESHGEDVGAEHESDRETSESHGDGGDVGAEHESDDRETSESHGDGGDAGEEYDSDWEYHHVSEDVSEDSLESDLLDDGDVEIPDNNHLLSESMDDGHMYSTPDRRQEKHQVDSLDVNIDSSPYKPAVSQMFNSGSEDKDSVAQNEEKSMNMHVSLPKNSNVKVSDENVTSNDETKKEINGARKRKNDAINQDLASPMKVTEVNGSSIREPEPEKKSLLEPEYENNSNNTRTLEAGLIIEDLPAGNKEMQRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZZ4</accession>
    <name>A0A8I6XZZ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZZ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0492430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0492430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0492430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0407990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0407990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0407990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0492430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0492430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0492430"/>
    </dbReference>
    <dbReference type="OMA" id="WHEIIDT"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF37">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="54"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0492430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="308" mass="34881" checksum="5AFF53E9DAAE2C64" modified="2022-05-25" version="1">MQDAARAACVSHAFKRSWRCYPNLAFNTKALGVDERSCGKDEIVRNFTSKVDNILNNHSCIAIKTLEIVFRYYDDKVCNLDSWLQTAITPGIEKLTVQLSTKSRKHYNFPCSLLASENGKLIQYLKLSCCAFSPTVGLCLKSLSRLELLNVNITGDQLGSLLHNAVALQRMKLNGCDYIICLKIPFHLQQLKYLEVFHCSSLRVIEIEAPNFSNFQFRGLRQVQLSLGVALQFKKFDMSFPGVVSYTCANLLSNLRYVEALSLCSWHEIIDTPVLPIKFLHLKHLSVDLNAMIFSPTYDVLLFSNILV</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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            <begin position="35"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39120" checksum="7E7B1199A820A8F7" modified="2022-05-25" version="1">MVHHGQSRGSPVATASRVRSSPPLSVESPSRRPSDPSSRPRAAEDPAATANPAPAAGGKPFLQAMPTVTEEAPPPLRAVQMEDVAMLAWDCLNRDDDDEELLRLLGNQTPLRDCHAFLDIGDITCAETLDPEESREPKRRRVLEYPSEVNQPDGRDHEMGSNFVTSEVTETSLICTDEPQSLNWDMQQDSDILDMISSLSYGAPYEPSDNQLENYSDGATVYYTPDQMTPSQESVTYMDYQTDMPGSSEIAPATENIIMQETSKLSVLKVSKGGSSMIKMKQNLTTFMTCPFTLIKPYWEEGNITLKDINQLIHAPPKQPPEILGMSAFSGKPVIGKTRIRTEGGKGSITILRTKG</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8I6Y3I4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571270.1">
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="NUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="14703" checksum="854BA91C062425CC" modified="2022-05-25" version="1">MLGVVDWNMKFLYVLPGWEGSSCDSRVLRDSMRTNRQDAFVLPHGKYYLADVGYKSGPGFLAPFRSTRYHFEEWASSQQHPQTAKELYNLRHSRARNVVQRTFGLWKKKWDSNHQCLLCATQLCEG</sequence>
</entry>
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    <accession>A0A8I6Y506</accession>
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        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y506"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0499330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF43">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="3">
        <location>
            <begin position="157"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="508" mass="55229" checksum="767F00959591A8FB" modified="2022-05-25" version="1">MSTMGVGSNNSHWSQTNDIHLDDHEFEVDEEGEGIIEAPKERAGNYTTNNDKLLCNTWLQVSRDPSVGGDQSRDAYWGRMKEHFDAQNVSGIDRSERSLRSRWSTINSDCQKWAAAQKAVDKLNPSGTNEDDRYNIAQNLFKEETRTTKKGKIKKGKIFTLPHCYEVLKDDEKWKKREDLDDLHLSNKRKRTIELNDDEEEDDASTDDGKRSPTPNSVSYSKPKRPDGCKKDKTEKKRRKGDDELTNAMEAIVKARKEANEVRKMARNQDAAAEERRLAAEERRVAAEERKVALEERKVGMEERAKLLEWENHLFFLDTSLFNDAQKEYVNLAREEVLIQKRAMIRTMGGGGLGGMGGGGLGTMGGMGLGAMEAMGGGVLGGMGGGGFGAMGGMGLGAMGAMGGGVLGGMGGGGFGAMGGFGAPSNAMGGMGGMSFASLMGGMGAPPAMGGMSFDVPHTHTHENAVEDLAKTVGATRDAVRDEVREEDSSSKAEESSSEEEEDDEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6G1</accession>
    <name>A0A8I6Y6G1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyprotein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0631410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF27">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="479"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="755"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1327" mass="149866" checksum="E2DBB48CC7EBFCC1" modified="2022-05-25" version="1">MSNVNLNFNAFLEKNKLKDDGSNYADWVRNLKLILEAAKKAYVLNAPLGDPPAPAAAQDVLNVWQARSDDYSLVRCGMLYSLETGLQRRFEQHGAYEMFQELKLVFQAHARVERYEVSDKFFSCKMEENSSVSEHILKMSGLHGRLTSLGVELPDDAIIDRILQSLPPSYKGFVLNYNMQGMEKTNPELYSMLKSAEVEIKKEHQVLMVNKTTSFKKGRGKKNFKKDGKAVAAPGKPDAGKKKKNGPKPETECFYCKGKGHWKRNCPKYLADKKAGNVKGICDIHVIDVYLTSARSSSWVFDTGVVAHICNSKQELRNKRRLAKDEVTMRVGNGSKVDVIAVDTLPLHLPSGLVLNLNNCYLVPDLSMNIVSGSCLMRDGYSFKSKNNGCSIYMSDMFYGHAPLVNGLFLMNLDRDVTHIHSVSTKRCKVDNDSPTYLWHCRLGHIGVKRMKKLHTDGLLESLDFESFDTCEPCLMGKMTKTPFSGIMDRATDLLEIIHTDVCGPMNVEARGGYRYVLTLTDDLSRYGYIYLMKHKSETFEKFKEFQSEVENQRDRKIKCLRSDRGGEYLSHEFGTHLRKCGIISQLTPPGTPQRNGVSERRNRTLLDMVRSMMSLTDLPLSFWGYALETATFTLNRAPSKSVETTPYELWFGKKPKLSFLEVWGCDAYVKKLQPEKLEPKAEKCVFIGYPKDTIGYTFYLRSEGKTFVAKNGSFLEKEFLSKEVSGRNVELDEVITPPLEQESSAAQEVVPVVPTPTEEEVNDDDHEASDQVTTEPRRSTRVRSAPEWYGNPVMEIMLLDNGEPSNYEEAMAGPDSNKWLEAMKSEIGSMYENKVWTLVDLPDDRRAIENKWIFKKKTDANGNVTVYKARLVAKGFRQIQGVDYEETFSPVAKLKSVRIMLAIAAFYDYEIWQMDVKTAFLNGNLKEELYMMQPEGFVDPKGANKVCKLQRSIYGLVQASRSWNIRFNEVIKAFGFIQVYGEACLYKKVSGSSVAFLVLYVDDILLMGNDIEMLESIKAYLNKSFSMKDLGEAAYILGIKIYRDRLRRLIGLSQSTYLDKILKKFNMENSKEGFLPVLQGMRLSKTQSPTTAADREKMSYVPYASAVGSLMYAMLCTGPDINLAISLVGRYQSNPGAEHWTAVKNILMYLKRTKEMFLVYGGDEELVVKGYVDASFDTDPDDSKSQTGYVYVLNGGAVSWCSSKQEVVAASTCEAEYIAASEATHEGIWMKELITDLGVVPSASGPMTLFCDNTGAIAIAKEPRFHRKTKHIKRRYNSIQDHVQSGVIDICKVHTDLNIADPLTKPLPRAKHDQHHNAMGVRYITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7A1</accession>
    <name>A0A8I6Y7A1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7A1"/>
    <dbReference type="SMR" id="A0A8I6Y7A1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0546750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546750"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000187">
        <property type="entry name" value="E3 ubiquitin-protein ligase ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF19">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="189"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="357" mass="37666" checksum="383D800FF6424793" modified="2022-05-25" version="1">MPPPPEDHPLPRPKPKPPKHKPKPKPAGTMKPEPVRDAAHPFPPDPLACAAHCSEPCAHHRLCAPSPPSRTATVHLRPSSRLPTPLIALSASVLAVSVLLLLVLLVCHVVRRRRRQVANVEPLHHQVVEQGLPVGMVVPAPAGDGSDDGVHHVWYIRTVGLDERAIAAITALVYDPDKCRALGLGGDGCAVCLAEFRGGETLRLLPRCGHAFHRGCIDTWLRAHVNCPLCRAPVKVTDGATPGDNVSSPVAAAAAGAEEAVGDGVLSTEMAVRRAASMVALPRLPWPDVSRRPLASNSAREGEMGLAKISRALKSWEALEMAGVGVGRSASFGAAPPRLPGRSGQPATSINADEIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7F7</accession>
    <name>A0A8I6Y7F7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7F7"/>
    <dbReference type="SMR" id="A0A8I6Y7F7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF544">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="15"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="308"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525240.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="341" mass="38381" checksum="CDEA0DCA14E3306A" modified="2022-05-25" version="1">MIISLEELEKATNNFDKARELGGGGHGTVYKGILSDLHVVAIKKPKKVVQKETDEFINEVAILSQINHRNVVKLYGCCLETEVPMLVYEFISNGTLYDHLHVEGPISLSWGDRLRILIETAKSLAYLHSTASIPIIHRDIKSVNILLDDTLTTKIADFGASRYISVEKSGLTTMVQGTVGYLDPMYFYTGRLTEKSDVYSFGVMVVELLTRKKPFSYLSPGGEGLIAHFAVLFAEDNLSQILDPQVIDESSREVPEVAKLAIACTQLKGEDRPTMRQVELTLESLQSSMHIVLDNVVDEEFENDIIPTNYPMNEHERGSDEDSRRQYSMEDELMISSRYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7N5</accession>
    <name>A0A8I6Y7N5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7N5"/>
    <dbReference type="SMR" id="A0A8I6Y7N5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0439000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0439000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0439000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0363480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0363480.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0363480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0439000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0439000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0439000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF661">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="1">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0439000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="18835" checksum="F83D7E892A5B93F6" modified="2022-05-25" version="1">MYNLVHHVAAGFNSAARGYRGPVWLAGAILLNPGFSRCTPSRSESAEVQVDPYMYYKMVGRFLALALPKGATRDHPYIWPVGDDAVAAALAVPPLLISVATLDTMRHRQVKYCNVMRRAGKDVEVALSPGVGHMFCLNQGAPGPADEDTAAHIAELVEAIACFVGRRHGYVARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9S7</accession>
    <name>A0A8I6Y9S7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9S7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0658290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035231317" evidence="2">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="9689" checksum="3D4DD25B7C0D43F6" modified="2022-05-25" version="1">MDRGSAGFAWLLVAGLLLGSWTTPACSSRLLPVPAAAADDDAKVWVAGGGPGYGGSDKLKAALLARRSLGLRLTAPPSPTSNTPRARAAPAPPGGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YA31</accession>
    <name>A0A8I6YA31_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YA31"/>
    <dbReference type="SMR" id="A0A8I6YA31"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000110">
        <property type="entry name" value="Argonaute 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000013">
        <property type="entry name" value="protein argonaute 10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="PAZ" evidence="4">
        <location>
            <begin position="227"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="5">
        <location>
            <begin position="502"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="823" mass="92403" checksum="454B9746A07CA0A3" modified="2022-05-25" version="1">MEQRVLAVPGNLPPASSKAEKFPARPGFGTIGRKCRVRANHFLVQVADKEIYHYDVAIDPETRSRGWNRSIINQLIKLYKEHLDGRLPVYDGRKSLYTAGALPFKNKVFVVKLANAAKGNQREEEYKVTVKLASNLDMYSLRQFLAGRNRELPQDTIQALDIALRECPTTKYVSISRSFFSQSFGHGGAIGNGVECWRGYYQSLRLTQMGLSLNIDISATAFYKAQEVMDFAFEYLNIRDASRPLIDRDRIKLKKALRGVRVDATHRKDKTIRYKITSISSAPLKELMFDQDGVRVSVVQYFKKQYNYSLKYINWPCLQAGSDSRPIYLPMEVCSITAGQRYSRKLNERQVSSILKMACERPAQRESSVLEIVNRNNYGNDDYSKEFGMKVINQLALVDARVLPAPRLKYHDSGREKVCNPSVGQWNMINKRMVNGGSINYWACLTFASRLNPNDIGMFCHDLARMCNSIGMDTVESAIRNIYGHSAQVLAEQGIKGKQLELLIIILPDVSGSYGKIKRLCETELGVITQCCLPKNVQKGGKQYLENLSLKINVKVGGRNTVLEDALYKRIPLLTDVPTIVFGADVTHPAAGEDASPSIAAVVASMDWPEVTKYKCLVSSQGHREEIIADLYTETKDPQKGLVGGGMIRELLLSFYRATGCKPHRIIFYRDGVSEGQFSQVLLYEMDAIRKACATLQTGYLPPVTFVVVQKRHHTRLFPENHRAWDVTDRSGNILPGTIVDTKICHPTEFDFYLCSHAGIQGTSRPTHYHVLLDENRFSADALQTLTYNLCYTYARCTRSVSIVPPGVLCPPGGLPCAILHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YA60</accession>
    <name>A0A8I6YA60_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123447204</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC38 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045123785.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979721.1">
        <property type="nucleotide sequence ID" value="XM_045123786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YA60"/>
    <dbReference type="SMR" id="A0A8I6YA60"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334980"/>
    </dbReference>
    <dbReference type="GeneID" id="123447204"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0279360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0279360.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334980.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd05804">
        <property type="entry name" value="StaR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033891">
        <property type="entry name" value="TTC38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16263">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16263:SF4">
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="476" mass="53337" checksum="51598888B579C78C" modified="2022-05-25" version="1">MAAAAEGEGEVRRDMWGQEYRTASADCAAALDDYYAQTMAFGRGRGPAALRAAAADPACALAALHAAHFVGPRDRAAAAAFLAAAAASLGKATEYERALFRALSALVGEDRDTEVAVDRHFQLLREFPRDLMSLRRAQLICFYVGRPDTSLEFVQQVLPENQDRNYVYGMLAFPLLELGRMDEAEIAARKGLAINKNDFWSQHNMCHVFQQECRFREATEFMESCSPSWTACSSFMFTHNWWHVAVCYLEGEAPIRKVMEVYDQNIMKELDRSDCEPAEVYLNALGLLLRLYVRGETGLAKERLTTLLDDLKNESIWHAEWLLDLLILWALSNMNEIKAAENLLTSVRSRVSSMDTKRQQVMHKAIQLADAVYKYGKGEHKAVFDTLGPDFDGLSYKMIGASDEQVDVFNEVWYTVLLNAGETSTAIELLVKQISKRQGAPFLWRLLEKAYTMEGRGADASVAAEKANALQAAHFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJC5</accession>
    <name>A0A8I6YJC5_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJC5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0585290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0585290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0585290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0585290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0585290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0585290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0585290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25891" checksum="64F8EF41ED6A7C9F" modified="2022-05-25" version="1">MSLCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEEPIHWSYGLGVTVDMTVQDAAYSMLTIMRARHALLQNSEFCHVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRERDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPTHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKG5</accession>
    <name>A0A8I6YKG5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary thiamine-phosphate synthase enzyme</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKG5"/>
    <dbReference type="SMR" id="A0A8I6YKG5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0746380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0746380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0746380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0746380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0746380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.460:FF:000002">
        <property type="entry name" value="Secondary thiamine-phosphate synthase enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF14">
        <property type="entry name" value="SECONDARY THIAMINE-PHOSPHATE SYNTHASE ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="14"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29387" checksum="44AD87E140500412" modified="2022-05-25" version="1">MIRRWMVFGRALSGYPHKTKGTRKEREEKKQPLQATTRSLAPLPASRFPGRRRRRRRRVRPCKRQRPPRRSRRPSSSPLCLPTGSGSSVRPRRAARSAVRCELVAAAAASASSAAPAPVAAPRWAQTTVVIPPQRRGCHIITHKIMHAIRSDLSEFKCGLAHLFLHHTSASLTLNENYDPDVQTDTETFLSRIVPEGPSAPWRHTIEGSDDMPAHIKSSMFGCALTIPITDGRLNMGTWQGIWLCEHRDYATPRTVVITLSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLD9</accession>
    <name>A0A8I6YLD9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Suppressor of forked domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123413441</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLD9"/>
    <dbReference type="SMR" id="A0A8I6YLD9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0553710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0553710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0553710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0667370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000137">
        <property type="entry name" value="Pre-mRNA-splicing factor syf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056350">
        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF33">
        <property type="entry name" value="SUPPRESSOR OF FORKED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="10">
        <location>
            <begin position="12"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="8">
        <location>
            <begin position="175"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="9">
        <location>
            <begin position="386"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="775" mass="87172" checksum="C33B8218ED0F92E7" modified="2022-05-25" version="1">MAAAMEIEVSADDVVYEEDVLRDPFRLSGWLRYLSGRVAAPPAKRAAIYERALRALPGSYKLWHAYLTELGDAARALPVTHAAHAGLNATFERALAAGMSRMPRLWHMYASSLLQQRLLTRARRVLDRALRSLPVTQHHRVWPLHLRLASLPDCPAPTALRMHGRHLQFDPDHAEEFIAFLVSAGRWRDAAHHLATAIDDEGFVSVKGRTKRELLLDLCGLLAQHPEEVAGMPVDAILRGSIRKFPEEASMLWTCLASHYARRGLHGKARDVFEEATTTATTVKDFRMVFDAYLHFEHAMAAAELELGQADTVDVQDCWLADRDSTDLTMARLERLLERRPELLNSVLLRQNPHDVQAWHERAKIFHKDPARHAATYVEAVKTVDPAKATGKPPHTLWLALTKMYEDRGSADCARDVLHRATQANFTAAEHLAAVYCEWANMELKHQNPETAIDLIRQATMPPSIEATMRTAAAGGEDVRAKLHRCLKLWLVYLDLMTTHGSLESTCAVYDKMHDLGVITPLLVLDHATLLEQHRRFEDAFRVYERGVRSFKHPHADAIWEAYLTKFVQRHGESRPERVRDLFQAAVLQAPPGRKKAVYLRYARFEEDFGLASRAMKVYEDAASAVPADDKLCVYEVYIARATELCGLLKARDVYRQAVVGGGLPDEGARAMCVGFADLEIGLGEVARARALYVHAASFTDPEVCPEFWKRWNDFEVLNGDESTFREMLRAKRTMAAVARDGAGTRRAIGTDGRLVECAGQRLDCAQQCKRIRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YN10</accession>
    <name>A0A8I6YN10_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123453001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985691.1">
        <property type="nucleotide sequence ID" value="XM_045129756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YN10"/>
    <dbReference type="SMR" id="A0A8I6YN10"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525430"/>
    </dbReference>
    <dbReference type="GeneID" id="123453001"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0436860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0436860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0436860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123453001"/>
    <dbReference type="OrthoDB" id="1932925at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF67">
        <property type="entry name" value="XYLAN O-ACETYLTRANSFERASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035301063" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="93"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="43392" checksum="C4903FAD90A348CF" modified="2022-05-25" version="1">MARLLAVLALVAAAAPFLRAAAQGDGELLPFAVGAAPAGCDVGQGEWVYDEAARPAYEEAACPYIPAELTCQAHGRPDMSYQHWRWQPRGCSLPSFNATVMLEMLRGKRMLFVGDSLNRAQYVSLLCLLHRAMPEGSSSFETVDALSIFRAKAYDATIEFYWAPLLAESNADDGVEHQLNDRLIRGAPMDRHSRFWKGADVLVFNSYLWWMTGDKIQILRGADNDMSKDIVEMGAEEAYRLVLHQVVRWLDGNVDPKKSRVFFVTASPTHASGQLWGDEAEGSNCYGQTAPIADASYWGSTSRAMLRVTGEVLGASARVPVGVVNVTQMSEYRRDAHTQVYSEQWAPPTKEQLADPKSYADCTHWCLPGVPDAWNELLYWKLFFPASDQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YND6</accession>
    <name>A0A8I6YND6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YND6"/>
    <dbReference type="SMR" id="A0A8I6YND6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="241" mass="28039" checksum="FE09D94DCCC5897C" modified="2022-05-25" version="1">MESYTLNEREHYEDVLRGPFLMELVHMMKLLIPEIKVTKRKSGFWGVTTKNFGRKGDPKTEDINYGTLTHDCDMGMNTVVHDAVARLSYRRRVELGTHYFGRLGWQKPEGAHIILTDEQKVKFSPVLVYNQELEHYIKNLQMNLLSELFENNALRESLKAEKLKVANVKKHDPEIQKLRQDLKNSQAMAHAGGVEIQKLKNQLLELQGQHRKIRVNGEYLAEELEKTKPDEKKPTDDEDMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNJ9</accession>
    <name>A0A8I6YNJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123411005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959856.1">
        <property type="nucleotide sequence ID" value="XM_045103921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704110"/>
    </dbReference>
    <dbReference type="GeneID" id="123411005"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0583980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0583980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0583980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0704110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411005"/>
    <dbReference type="OrthoDB" id="2019938at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04895">
        <property type="entry name" value="ACT_ACR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04897">
        <property type="entry name" value="ACT_ACR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040217">
        <property type="entry name" value="ACR1-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096:SF6">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT_ACR9_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="113"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="313"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="433" mass="47647" checksum="1BA87750DD567B08" modified="2022-05-25" version="1">MEWLNEYEKLVIGMDTPKVVIDNAVCPTATLVQVDSARKRGVLLEAVQVLADLDLSINKAYISSDGRWFMDVFHVTDRLGRKLTDDSVITYIQQSLGTWNGPSQPASLEGLTVLELTGTDRTGLISEVFAVLADMSCSVVDARAWSHRGRLACLVYLRDEDVAAAGVERIEARLAPLLRGDSEASGGAVAAVPAGSIAHADRRLHQLMFASRDHEHAFPTPWVSVESWAERGYSVVTVQCPDRPKLLYDVVCTLTDMDYLVFHGTIDTNAGQARQEFYIRHADGSPISSEAEMQRVSQCLQDAIERRSFEGVRLELCTPDRPGLLSDVTRTFRENGLLVAQAEVSTKGDMASNVFYVTGTTAGQAVQQNAIEAVREKVGVDCLVVEEHRPRLYQKARGQDDRDDRNGAGIGLLYLGNLVMRNLYNLGLIKSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQ46</accession>
    <name>A0A8I6YQ46_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123401164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950830.1">
        <property type="nucleotide sequence ID" value="XM_045094895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQ46"/>
    <dbReference type="SMR" id="A0A8I6YQ46"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553150"/>
    </dbReference>
    <dbReference type="GeneID" id="123401164"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0553150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401164"/>
    <dbReference type="OrthoDB" id="2011965at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="457" mass="51187" checksum="AB4F346A29F15F43" modified="2022-05-25" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRW0</accession>
    <name>A0A8I6YRW0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRW0"/>
    <dbReference type="SMR" id="A0A8I6YRW0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0463460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0463460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0463460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0557880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF160">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="445" mass="48489" checksum="7C25F5899CD87EEB" modified="2022-05-25" version="1">MSHFFQFPGKTSHRSYWPAPPQNIPASLFCAYRRPSSGGGFGDVVAAFEAKLLSLLDHFLPLTGRIVVDPRSGRREMLNCDNQGAELVLAEAGVALASLDYGQLGASLAEVGVPIKYDAGVALSVQLVSFSCGGFAVAWASNHVLTDGYSLCMIASAWSEVARSGTLSAAVNHDRSVFRPRAPPSYSPSFGEQFSPLEGEHLVNALTTQCSFVERTYYVEARDLETLRSQASQDGVVATRLEAVSAYLWKAFAAVVGASDESCRMGWWVNGRPRLAAPRYKAAMREREYIGNVTTLAVAEARVEDTRRLPLPDIASTVRETIRSTATDEHFQQQVDWVEEHRATKYVETACVGLGSPAVTVTSFATFSLDTDFGFGRAALTMPTGEDCGRLCSGFVQIIARPGGDGWLVDMYVWPRLAAALDSDECRIFKPLTAEFLGLGHYRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YS34</accession>
    <name>A0A8I6YS34_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Powdery mildew resistance protein 12-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YS34"/>
    <dbReference type="SMR" id="A0A8I6YS34"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0464850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0464850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0464850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0559510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19338:SF76">
        <property type="entry name" value="RX N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19338">
        <property type="entry name" value="TRANSLOCASE OF INNER MITOCHONDRIAL MEMBRANE 13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="173"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30563" checksum="948DF938895A21A4" modified="2022-05-25" version="1">MSAQIVSAAIGVMNPLISKLATLMGDKYTKLTGVRRQASFLKDELSTMKALLEKLELMDELDPLAKNWRDHVREMSYDMENCIDDFMRDLGGTDVKTGFIKKTARRLKTLRKRHRIAHRMEELKVLVLQANERRMRYKIDDCINSTSRIVSVDPRISAIYKQAAGLVGIDGPKKELVSCLTETQDNLKVVAIVGFGGLGKTTLAKQVYDEIGGQFGCKAFISISQRPDMASLLSGLQLKLGMKEESSHAHEVQDIIGCLREHLTHKRYYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YUU7</accession>
    <name>A0A8I6YUU7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123398122</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948562.1">
        <property type="nucleotide sequence ID" value="XM_045092627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YUU7"/>
    <dbReference type="SMR" id="A0A8I6YUU7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0506940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0506940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0506940"/>
    </dbReference>
    <dbReference type="GeneID" id="123398122"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0420980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0420980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0420980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0506940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0506940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0506940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398122"/>
    <dbReference type="OrthoDB" id="2355at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103118">
        <property type="term" value="F:UDP-3-O-[(3R)-3-hydroxyacyl]-glucosamine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001289">
        <property type="term" value="P:lipid X metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03352">
        <property type="entry name" value="LbH_LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007691">
        <property type="entry name" value="LpxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01853">
        <property type="entry name" value="lipid_A_lpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002060">
        <property type="entry name" value="PRK00892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43378">
        <property type="entry name" value="UDP-3-O-ACYLGLUCOSAMINE N-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43378:SF2">
        <property type="entry name" value="UDP-3-O-ACYLGLUCOSAMINE N-ACYLTRANSFERASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0506940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="281" mass="29524" checksum="5AF3C856673C0EAC" modified="2022-05-25" version="1">MAAILPRTALLLRTPRGAGRLARFLGSGVPAASSSDSETVASAAAEFMPWRNGGGVLHQAASVDPSAVVEAGAVVHSGAVIGKEVVVRSGTVVGPSVSVGQSTRIGYNVVLSNCSIGEFCTIHNGACIGQDGFGFFVDQDGQVKKKPQELYARIGDNVEIGANTCIDRGSWRDTMIGDDTKIDNLVQIGHNVVIGKCCMICGQVGIAGSATLGDYVTLGGRVAVRDHVSIASKVRLAANSLATKDIQEPGDYGGFPAVPINEWRRQTVNLRLFSKKHHDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVC8</accession>
    <name>A0A8I6YVC8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123452319</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984897.1">
        <property type="nucleotide sequence ID" value="XM_045128962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVC8"/>
    <dbReference type="SMR" id="A0A8I6YVC8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0511960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0511960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0511960"/>
    </dbReference>
    <dbReference type="GeneID" id="123452319"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0425260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0425260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0425260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0511960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0511960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0511960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452319"/>
    <dbReference type="OMA" id="CRNGCLG"/>
    <dbReference type="OrthoDB" id="595188at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1228">
        <property type="entry name" value="NB-ARC DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="154"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="419"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="610"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0511960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="932" mass="103450" checksum="873EAE45D49A34AA" modified="2022-05-25" version="1">MVDVVTGAMASLLPKLEKLLEHKLHRTVKEDAEQLLRELTSLHAALGSGPDPRWADDCRELSYHADDIVDAYLAASEPAACRDGCLGFLDNLLGPRRWFANALKDVKSLALHLSDRYSSTDKDKLVPATAPTGPSPHLWKEAEEPLVGMERGRDTLIKSLAGGQEMKIVSIVGSGGLGKTALANLVFRQLRPQFGSCAFVSVSSMPDMEGIFDQMLRQLNVEKNYNQEAKDAVRDFLQSERYLIIVDDLWRKEDWHILSRSLPVNAFGSRVIVTTRVNDIAKICCSGQDELICEIKHLDHLNSKSLFLRRCFGSEASCPDALAREVGEILAICSGIPSVIITIAELLNSKVATRKPCQDVVHAFCSAWKEMASSLHSARERIPGLEDLIKVLSLSYENLPSTLKTCLLCIATSTTVKYRKFKRGETVRKWIAEGFISEVGWRSSEEVANECFDDLVGSKVIQPVERPSFYGKGMYEVNCMMLYVLRLIAREENLVTFLSDMDISETPATRPVFLSIRCCGSNGLNGTERLDMSHVRSITMIRCDKVFTFKHLGYLRVLNVEDCDGLDDTDVHHICGMILLKHLSLKETPQVTRLPPQIGNLRHLETLEIELTQISVLPPQIGKLQNLETLDVRMTGVKELPEELVMLPKLANLHFGRTGVRLPAGSDRFKSVKVLGTIDSRECSASTLEEIREMTGLTKTEVMLYDEPADVPKNDNLLSCMGNCTSLRSLIVHGDFITSDDLPASPNFPLLEKLTVATRLVKFPGWIAHLSRLKKLEIRLFEQGLNDLKILGDLPSLTSLAFQLFGNPRKQVAITSGFARLEFFAFDYSAPWVKFDQGAMPSLRHIELTHYSSGPAGKSPAGIIYLESLQKVTLIYSSHCSSCAGVIKTVAVMRKEAASHANRIVLSVNGDDEIFPSISSVDGKITTTENEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z4I1</accession>
    <name>A0A8I6Z4I1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123450433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z4I1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0687500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0687500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0687500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0687500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0687500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0687500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF492">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0687500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="16187" checksum="1431892D38BD536D" modified="2022-05-25" version="1">MASGPNVGGNGGGGGGGGGLMKNMSLLRLQYYCVLGAVAGAVLFATLRYMPAAGSSVALSTTSALATAAASAGAGEHRKSKGMAQAPTKAAAKVATKRKEVVVFNFGDSNSDTGGVAAIMGIRIAPSEGRAFFHHPTGMLSDGRVVLDPHCAVVLHLARLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z8Z6</accession>
    <name>A0A8I6Z8Z6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123407661</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044956782.1">
        <property type="nucleotide sequence ID" value="XM_045100847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z8Z6"/>
    <dbReference type="SMR" id="A0A8I6Z8Z6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747080"/>
    </dbReference>
    <dbReference type="GeneID" id="123407661"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0619720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0619720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0619720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407661"/>
    <dbReference type="OrthoDB" id="656061at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="USP_STK_Ubox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF142">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="280"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="168"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="605" mass="64735" checksum="0895881F81907B2F" modified="2022-05-25" version="1">MEEEEVYCAVGKEQWNWKANLRWVLANFPGRRLVLAHVHRPPHRINMMGAWVPASQVGAAMVAACRKWEEDEASEALDHLLHICKAHRVDARKLIVSGDDLPGGLVRLVADHGVAELVMGAAADRAYSRKMRAPKSKKAAAVKLMANPSCRIWFVCKGKLICTREASDEGLMSRGEPSTASTSPRPTASDDGSTSKSSSSRHGGGGAEPAGMECQRDKSFLDGEKSVRSDQGDASEMEDQQTRRPPEAAEEPMPAAVDGDQGVDFLRLGLPELEEATGRFDESARIGSAGIGRAGVYRGSLRGMSVAVTVISPDVAVGEARFAREVEAIAKVRHPGLVQLVGACPEARAVVHELVPGGSLEDRLEQGGDGTPPLPWLARCRVAYQVCTALAFLHSSAKTVHGDVRPANILLLDDEEHRPSGSKLADLGVRGLMAQAEQQRAGREALAYVDKRYLATGEPTPQSDVHALGLLLLRLVTGLPARWAKKAALEANAGRGRSWHQVVDASAGGWPTELATEVALLGLRCCAVSDGRTPCRPAGELLEEARAVLEAAMDAAPGRTWSSSLLSSSEKEASNGGVARLVLLHLPDNNQDQSNLRANSSRSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZB55</accession>
    <name>A0A8I6ZB55_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">[RNA-polymerase]-subunit kinase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZB55"/>
    <dbReference type="SMR" id="A0A8I6ZB55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0704000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF486">
        <property type="entry name" value="OS12G0427000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="340" mass="37451" checksum="6B2DF0D9F6454636" modified="2022-05-25" version="1">MAASAGKKEAAWPLTMAQYERLEKLGEGINGEVFKAWDTEDNLIVAVKRLSGSGDDGFIISGLPEVMREAMCLGACRGIPSIVQRRATCVAACQREASDSFIVMDYVGRLNLRGYMQPRVRRRRPFSEDEVRRIMKQLVEGVTAVHAVGVLHLNIKPENVLLDDGTEDRKQKPKKGPVEADVSGELKEDRIVYKIGGFGMSMKGRSKRQPEVTILTPYSAPELLLHSCEYDDRVDTWGLGCIMADLLSDTGVSTFDGESDIEIMAKVFGIVGTEGIKDWSGYSGSKLSAAGFEVLNGLLESNPEKRLSAAEALQKPWFHNRRRGFAGFFKSCVVGVLPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2L3</accession>
    <name>A0A8I7B2L3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123429866</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044969777.1">
        <property type="nucleotide sequence ID" value="XM_045113842.1"/>
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    <dbReference type="SMR" id="A0A8I7B2L3"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135680.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0111800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0111800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0135680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135680.1"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MYB-related transcription factor"/>
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    </dbReference>
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        <property type="entry name" value="Transcription factor MYB39"/>
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        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
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        <property type="entry name" value="SANT/Myb"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="353" mass="38904" checksum="3ABD1C329A1B6432" modified="2022-05-25" version="1">MGRAPCCDRTGLKKGPWTQEEDEKLVAYIKKHGQGNWRTLPKNAGLERCGKSCRLRWTNYLRPDIKRGRFSFEEEEAIIQLHSILGNKWSAIAARLPGRTDNEIKNYWNTHIRKRLLRMGIDPVTHAPRLDLLDLSSLLKPAAYYPTQADLDTLRALEPLANYPDLLRLASTLLSAPTTTTSQSQPTSEHQMLLPWLIQAQMAQQQVSHSQAPPQHATAADMFMQPSSASACQMPGLAHANQAQQLHQDHMVASDYGQLLSYDNELDYMPALMQMASDTSNLQWSSTVTSSNNCNVGSGVSTPSSSPAGRVNSASTAAFCANTSNIDAISADAAGLFVDMHLSDCLLDVSDYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4M0</accession>
    <name>A0A8I7B4M0_HORVV</name>
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        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4M0"/>
    <dbReference type="SMR" id="A0A8I7B4M0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0103780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0103780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0103780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0085240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0085240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0085240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0103780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0103780.1"/>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000089">
        <property type="entry name" value="DNA repair protein RAD5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056450">
        <property type="entry name" value="UBA_RAD5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24559">
        <property type="entry name" value="UBA_RAD5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="565"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="928"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="999"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0103780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1164" mass="129923" checksum="56B85AEB0ECF1F20" modified="2022-05-25" version="1">MTCDGVWQESPSQAELFAAEVAAIRAVLGAALPEARVLAALSRCGGNADRAINALLDDGDTDADAAAERAIDELLDAENVRAKAERDAALTLATKFRVKTEAVDVPKKERPLCPPPAKVAPLPPPRRVKEEEEEVTSGRPRSRAGGCGISLVPRPVKMDCDSDEVEVIDAAPRSKKRVREEEDVVQLTATHPLPYLNPRPIRAIPPEEAARMYDPQPIRVVPPREAAKMHTPPQRQRPQMASAIAAPPKSDWKMVVAAPEAELGDFPLEPDWFLVDKSYVAGLSTHSGRKMLDAGEIVHFGFPSYDRANCGIKMSAKKAASLLQIVRFSTKRAGEIGKLSPEWTKCLVPLVNSSKVKIQGKIVFQTMELRLMQDILLYVSFYIHKSVFTEGDNSSLSQLAPANVDYSDNPLHALFKLLKLRASVKADFTLDELTRKRPWNLRGDTNGDDESTPIVGLETRRTAGQTFPEQAADEQAISEAALNKIIGTAEIYDLKEAEPPRTLVSVLKPYQKEALFWMSELEKGCIDDESKNAIDPCFSAYTIADKRAPAVYINVFSGEATTKFPSLSKTTRGGILADAMGLGKTVMTIALILSNPRGEHSDYIERDIIRPVRGRDTRTRTSTPSIRGGTLIVCPMALLGQWKDELEAHSTPGAISVFVYYGGDRTGDLKLMAEHTVVLTTYGVLQSAHKADGSSAFHRIDWYRIVLDEAHTIKSPKTKAAQAAYMLSSQCRWCLTGTPLQNNLEDLYSLLCFLRVEPWCNSNWWQKLIQRPYENGDERGLKLVKAILRPLMLRRTKETKDKMGKPILVLPPANIEVVECEQSIEERDFYEALFRRSKVQFDKFVAQGNVLNNYANILELLLRLRQCCDHPFLVISKADTKKYTDLDELAERFLKGARNDPGCRAIVPSRAFVEEVVEEIRQGTAAECPICFESTSDDPVITPCAHRMCRECLLSSWSTPAGGACPICRSPITKADLIMLPVQCRYEVDAKNNWKDSCKVVRLLATLKDLGKKGEKSIVFSQFTSFFDLLEIPLNHKGIKFLRFDGKVTQKHREKILNEFSQSKDKLVLLMSLKAGGVGLNLTAASNVFLMDPWWNPAVEEQAIMRIHRIGQKRAVQVRRFIVKDTVEERMQQVQGRKQRMIAGALTDEEVRSSRIEELKMLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6M6</accession>
    <name>A0A8I7B6M6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123446844</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044979316.1">
        <property type="nucleotide sequence ID" value="XM_045123381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6M6"/>
    <dbReference type="SMR" id="A0A8I7B6M6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332040"/>
    </dbReference>
    <dbReference type="GeneID" id="123446844"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0276650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0276650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0276650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446844"/>
    <dbReference type="OMA" id="CKEAKRQ"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:epsilon DNA polymerase complex"/>
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    </dbReference>
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        <property type="term" value="F:chromatin DNA binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22928">
        <property type="entry name" value="HFD_POLE3_DPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000090">
        <property type="entry name" value="DNA polymerase epsilon subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="DNA_Pol-Epsilon_Subunit"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="105"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="168"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="211" mass="22137" checksum="82E927110F1A5A58" modified="2022-05-25" version="1">MAGEAKEPQADGSKPKVKKPKAAAAVAQDGDGAKVKAKKPKAGQEGDGAKAKKAAAAVAAAPVAAMTEAEVEELPKAIVRRLVKDKLARVASGGEGAEGGAEVIVNKDAMAAFAESARIFIHYLSATANDVCKDGKRQTINAEDVFKALDEIEFPEFVEPLRTALEEFRSKNAARKPASGKKQAEKKRKLDKEAVPEEENGAADETNAAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6Z0</accession>
    <name>A0A8I7B6Z0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123446199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978813.1">
        <property type="nucleotide sequence ID" value="XM_045122878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6Z0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0350810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0350810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0350810"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0350820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0350820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0350820"/>
    </dbReference>
    <dbReference type="GeneID" id="123446199"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0292610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0292610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0292610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0350810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0350810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0350810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0350820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0350820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0350820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446198"/>
    <dbReference type="KEGG" id="hvg:123446199"/>
    <dbReference type="OMA" id="TSNDNAH"/>
    <dbReference type="OrthoDB" id="747111at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33983">
        <property type="entry name" value="OS07G0185900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33983:SF1">
        <property type="entry name" value="OS07G0185900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0350810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="80" mass="8391" checksum="2219988661654E5F" modified="2022-05-25" version="1">MGKYVELLDMGVRIAVRFHSHCPQTARFYYHPPASSAAPGAGDGGGMVPAGGTVAGALAMMKRQQRAAVDATKIILYTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6Z8</accession>
    <name>A0A8I7B6Z8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123427075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
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        <property type="nucleotide sequence ID" value="XM_045111026.1"/>
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    <dbReference type="SMR" id="A0A8I7B6Z8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0177270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0177270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0177270"/>
    </dbReference>
    <dbReference type="GeneID" id="123427075"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0146670.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0177270.1">
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
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        <property type="entry name" value="OS04G0488400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0177270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="19454" checksum="0D96B631B75F7B13" modified="2022-05-25" version="1">MSREALAIGHVVGDILDPFVKAASLKVMYNGKELTNGSELKPSQVATEPRIDIAGRDMRNLYTLVMVDPDSPSPSNPTKREYLHWLVTDIPESTNASYGNEVVSYESPKPTAGIHRFAFILFRQSVRQTIYAPGWRPNFNTRDFSALYALGPPVAAVFFNCQRENGCGGRRYIR</sequence>
</entry>
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    <accession>A0A8I7BB95</accession>
    <name>A0A8I7BB95_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
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        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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    <reference evidence="2" key="1">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
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    <dbReference type="SMR" id="A0A8I7BB95"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0326890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18183" checksum="FCC2A3694889BA3C" modified="2022-05-25" version="1">MKREQEAASLREKIDAMVQSNELMLLKSLEIKKELDEKKTKEKQEKWQMLKEEGLRKAAIEKQKARTSETKSMSLLLVEENKIMSMNRNDMDDLTKTWHDMARREILKRRMVASTGACSSSGDVFSAPYAANVDDFSAGVGTSVGCGFGGADELQYGLHSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCY6</accession>
    <name>A0A8I7BCY6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0400380.1">
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22889" checksum="9FDA1A6DEDFE4FB1" modified="2022-05-25" version="1">MAHLKFTNIPEAHIMSRWTKNACEDLPEHLKIYKGRTPILDSTTFRHTTLYTTALDIVKMGDSNPEAFAFAMSRLSDTRVGLKEKAKEQDGKGLEDVLHKENYDCQRKFNGDTGLKSSMSVEFRKRDRGRPTNSRSRPGYEVSKPRTKFCKTFRAKGHNCGNCPTNGGPNKKQRKEPKCGKCGLLGHIRTQCSRGFESWFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDN3</accession>
    <name>A0A8I7BDN3_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="primary">LOC123396408</name>
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    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044947287.1">
        <property type="nucleotide sequence ID" value="XM_045091352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDN3"/>
    <dbReference type="SMR" id="A0A8I7BDN3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0427920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0427920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0427920"/>
    </dbReference>
    <dbReference type="GeneID" id="123396408"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0355460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0355460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0355460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0427920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0427920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0427920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396408"/>
    <dbReference type="OrthoDB" id="665356at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF50">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="546"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="579"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="454"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0427920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="636" mass="69737" checksum="A3EF4994F5D7E629" modified="2022-05-25" version="1">MQASESPKEAKLEVQHHQPELLMAARTGNLDELRLILGRDNTTVTPVVLEPVVTIDIERVDMDVERVATLEMDSILHVVAASGDSPKFLNCASMIHEKAKHLLDARNSNGDTPFHCAARAGGVNMLTHLIGLARADGDDARVTGVLRKQNKKGETALHEALRLADKEILKNMVCILMQEDTELACLPHANDTSPLYLAVSLGHDDIAYVLHLKNNKLSYSGPHGQNALHVAVLRGKEITKKLLDWNTHLTKQADQCTGSTPLHIAISWGSQSKDVIKLLLTHNKTAAFQRDNSGLFPIHVAAMRRSWSTLRVLLDKVPECVGLRDGNGQTFLHVAIEKEHPLVVGSWCHHKSIINVQDNHGNSPLHLAAKVGNQWIFYLLIQNPQVQLDLVNNEGQTPLDIAWTKMPQGLNFLLNPRNRIYLLLKGAGAKTAAYRCDLFLKEHIPLIDIKLEEKKISDSTQIIGIGSVLIVTVAFAAAFTLPGGFRTDDLKGKHGTAGIAMLAGKPVFHAFIIANTLALVSSALATMNVMFAGVTAVDIRTRMSAFIISIVFVYCSAKSLAAAFVFGLYVVLAPAAPKIAYISCAIVAPFLFLDVVWFIFMVAIGEVMLLKRLGFKAWLRNFNFSRLPVKQPTISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEG9</accession>
    <name>A0A8I7BEG9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0010073">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF87">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478060.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="110" mass="12109" checksum="02A37865472C4A51" modified="2022-05-25" version="1">MIATLQDVSMITALPIEGKPLCMSTDSEGWRQQMEALIGMSLQEPEVEDGGKKDRVPAGAPFTWIAANFAHCPEDADDEVIQRYARVYMWYVISRTIFADGTGKNAHGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BHW0</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0632790.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="132" mass="15030" checksum="10C1699F5EC61FA8" modified="2022-05-25" version="1">MKENGENLHWSAWHGKASSVSIPSSTFTSLAYTPRPYPPFLINHQSRSDDRRRKLQGLREGPTGQTHRPIVRRFFPAFIKRPKQASPNRRAPFFFSKSFSPCTPAPPPSPFLLVVEGRRCRLAVIDCSRLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BI81</accession>
    <name>A0A8I7BI81_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123409813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958620.1">
        <property type="nucleotide sequence ID" value="XM_045102685.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I7BI81"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648800"/>
    </dbReference>
    <dbReference type="GeneID" id="123409813"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0648800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409813"/>
    <dbReference type="OMA" id="ISSNCRC"/>
    <dbReference type="OrthoDB" id="664799at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00261">
        <property type="entry name" value="AAI_SS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006106">
        <property type="entry name" value="Allergen/soft/tryp_amyl_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006105">
        <property type="entry name" value="Allergen/tryp_amyl_inhib_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34481:SF10">
        <property type="entry name" value="CHYMOTRYPSIN INHIBITOR WCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34481">
        <property type="entry name" value="TRYPSIN/FACTOR XIIA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00808">
        <property type="entry name" value="AMLASEINHBTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00426">
        <property type="entry name" value="CEREAL_TRYP_AMYL_INH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035282618" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="15209" checksum="A378106A3099BB2C" modified="2022-05-25" version="1">MASCSQHLLSAVAIFSVLAGVATATSIYTCYEGMGLPVNPLQGCRFYVASQTCGAVPLLPIEVMKDWCCRELAGISSNCRCEGLRVFIDRAFPPSQSQGAPPQLPPLATECPAEVKRDFARTLALPGQCNLPTIHGGAYCVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQ99</accession>
    <name>F2CQ99_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401785</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK353801">
        <property type="protein sequence ID" value="BAJ85020.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK369782">
        <property type="protein sequence ID" value="BAK00983.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951571.1">
        <property type="nucleotide sequence ID" value="XM_045095636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQ99"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546570"/>
    </dbReference>
    <dbReference type="GeneID" id="123401785"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0454310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0454310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0454310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0546570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401785"/>
    <dbReference type="HOGENOM" id="CLU_092224_0_0_1"/>
    <dbReference type="OrthoDB" id="1903669at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010275">
        <property type="term" value="P:NAD(P)H dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014946">
        <property type="entry name" value="CRR6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038024">
        <property type="entry name" value="CRR6_slr1097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35724">
        <property type="entry name" value="PROTEIN CHLORORESPIRATORY REDUCTION 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35724:SF1">
        <property type="entry name" value="PROTEIN CHLORORESPIRATORY REDUCTION 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08847">
        <property type="entry name" value="Crr6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ85020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK00983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="259" mass="29036" checksum="84BAE9F1B781BC94" modified="2011-05-31" version="1">MAAAAAVHHHHHPIVASLRPASRRLSSSSAGSAPPHPAASFLCCCSRPETPRVRLQAPRVAFNPSGNFDLSLSDQDDAPQVEPPPPPTEGRIEIVINKDIIRTLDLSPVQEALGNLNSLTTAQSRNLLDRTVGFTINYEREDPYDTRELSEFPDVRLWFVRLDASYPWFPVVLDWRAGELARYAAMLVPHQMSMRHGVVFNPEALELFGMKKVFVVYSWLKQQNHAKPRLKTGDMAKMLGFGIGDELFDLIEKYPVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F2CW22</accession>
    <name>F2CW22_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123443036</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May only reduce GSH-thiol disulfides, but not protein disulfides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CGFS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK355825">
        <property type="protein sequence ID" value="BAJ87043.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975193.1">
        <property type="nucleotide sequence ID" value="XM_045119258.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975194.1">
        <property type="nucleotide sequence ID" value="XM_045119259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CW22"/>
    <dbReference type="SMR" id="F2CW22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241230"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241230.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241230"/>
    </dbReference>
    <dbReference type="GeneID" id="123443036"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0200290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0200290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0200290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0241230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0241230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241230.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443036"/>
    <dbReference type="HOGENOM" id="CLU_026126_3_3_1"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CW22">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="9">
        <location>
            <begin position="89"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ87043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="185" mass="20071" checksum="BA0FBE0E1A357D0A" modified="2011-05-31" version="1">MARLVSSSLVRGLVRSCRASSTAAVSRPAFQQFMTYSSGISGDSNVNGGTSTTRVTADSDTHQDFQPTSKDSDMSLQDIVAQDIKENPVIIYMKGHPQAPRCGFSALAVKVLQQYGVSITSRDILTNMKLKESVKAYSNWPTFPQIFINGEFVGGSDIILSMHQKGELKELLGDSAQKGEQDSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZ99</accession>
    <name>F2CZ99_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123410000</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356955">
        <property type="protein sequence ID" value="BAJ88170.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371119">
        <property type="protein sequence ID" value="BAK02317.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958805.1">
        <property type="nucleotide sequence ID" value="XM_045102870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZ99"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676350"/>
    </dbReference>
    <dbReference type="GeneID" id="123410000"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0561270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0561270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0561270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0676350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410000"/>
    <dbReference type="HOGENOM" id="CLU_095143_0_0_1"/>
    <dbReference type="OMA" id="GSIMGCV"/>
    <dbReference type="OrthoDB" id="666653at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CZ99">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33430">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33430:SF6">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ88170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK02317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="20220" checksum="316B7FB27CAA9414" modified="2011-05-31" version="1">MDESDERGNGGGGHHGYEWKLPAALSANTTSVHVTALDGVVNVNSLFTVAVFVGLSLATPGELRSLAGDSSCDAGPEVARSLLVLEVVAFSSFLFSSLVAQGLKLAINLINSKDPDDTHAHIDARLLRLGMLASAVGSVVGCVFLMASMVMVVQIRLGTLGCASNRAAAKAAAGLVGLVSTALVVYISTVFYTFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0M7</accession>
    <name>F2D0M7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123411567</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357434">
        <property type="protein sequence ID" value="BAJ88648.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960470.1">
        <property type="nucleotide sequence ID" value="XM_045104535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0M7"/>
    <dbReference type="SMR" id="F2D0M7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748130"/>
    </dbReference>
    <dbReference type="GeneID" id="123411567"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0748130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_25_4_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF906">
        <property type="entry name" value="OS08G0205100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="174"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="431"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="554"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ88648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="704" mass="80221" checksum="8CA60AAC5EBBAEED" modified="2011-05-31" version="1">MAAEALVSVSTGVMNSVLEKLATLMGEEYTKHKSMQREVAFLKDELGSMDAVLKKLESIEEELDPQTREWKNQVMEMAFHIEDCIDNFMHESGEDSNRGGVGFVGKIAQYINALRTRHQFANQIQQLKTLVKDVSERRKRYKLDECASSSSYVAVDPRVPALYTEAANLVGMEGPKEDLIKLLMAEEAESVQPLRVVSIVGFGGLGKTTLANQVYHELGGQYDCKVFVSISQRPNMMKLLGRIIKKLKMPQATHTDEVQDLIDSIREYLREKRYFFVIDDIWDESVWGIIRCAFPENQQGSKVITTTRIEMVAKATCNFQHEFVYKMSPLDDQNSRKLFFSRVGQVDLQPLEEISIEILKKCGGLPLAIVSIASLLVSQPTRSVTQWKYVCNSLSSNLRTNPTLEGMRQVLNLSYNNLPHRLKTCLLYIAMYPEDYIIVKADLLRQWVAEGFIHKVHGQDTNKVAESYFNELANMNMIQPTNIWYDGQVIECKVHDMILDLIRLKSEEQKFLSVVENLQGMIPALQSKVRRIFLQLDCGANECIVQASLSMPYIRSLALFGKSPFKFSMYELKSIRVLNVHYEGGLIADRIDLTPISNLFHLRYLSINSETKKKYMSISSQKEKMPILILPTLIGDLKHLETLESCMDITAMPCDIVHLPCLMYLHVPMGTRLPDGIRRMKALRTLVNFDVHMNSVQSETSLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2D376</accession>
    <name>F2D376_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123394951</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
            <tissue evidence="4">Shoot</tissue>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK358333">
        <property type="protein sequence ID" value="BAJ89547.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366174">
        <property type="protein sequence ID" value="BAJ97377.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK373473">
        <property type="protein sequence ID" value="BAK04670.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945792.1">
        <property type="nucleotide sequence ID" value="XM_045089857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D376"/>
    <dbReference type="SMR" id="F2D376"/>
    <dbReference type="STRING" id="112509.F2D376"/>
    <dbReference type="PaxDb" id="4513-MLOC_66309.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0449030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0449030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0449030"/>
    </dbReference>
    <dbReference type="GeneID" id="123394951"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0371950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0371950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0371950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0449030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0449030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0449030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394951"/>
    <dbReference type="OMA" id="GINNGMH"/>
    <dbReference type="OrthoDB" id="10265171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D376">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007726">
        <property type="entry name" value="SS18_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05030">
        <property type="entry name" value="SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SS18 N-terminal" evidence="3">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ89547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ97377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK04670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0449030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="19545" checksum="022BB3070F7BCD81" modified="2011-05-31" version="1">MQQAMPMPPAAAAPGMPPSAGLSTEQIQKYLDENKQLILAILENQNLGKLAECAQYQAQLQKNLLYLAAIADTQPQTSVSRPQMAPPAASPGAGHYMSQVPMFPPRTPLTPQQMQEQQLQQQQAQMLPFAGQMVARPGAVNGIPQAPQVEQPAYAAGGASSEPSGTESHRSTGADNDGGSGLADQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2D899</accession>
    <name>F2D899_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123443127</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK360111">
        <property type="protein sequence ID" value="BAJ91320.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975335.1">
        <property type="nucleotide sequence ID" value="XM_045119400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D899"/>
    <dbReference type="SMR" id="F2D899"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244910"/>
    </dbReference>
    <dbReference type="GeneID" id="123443127"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0244910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443127"/>
    <dbReference type="OMA" id="DIQAGHF"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D899">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="120"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="462"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
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            <dbReference type="PROSITE" id="PS50929"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="BAJ91320.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244910.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F2DIN4</accession>
    <name>F2DIN4_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402607</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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            <begin position="58"/>
            <end position="77"/>
        </location>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ94955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580890.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2DKZ4</accession>
    <name>F2DKZ4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123401011</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
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        <property type="protein sequence ID" value="BAJ95765.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045094728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DKZ4"/>
    <dbReference type="SMR" id="F2DKZ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582480"/>
    </dbReference>
    <dbReference type="GeneID" id="123401011"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0482920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0482920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0482920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0582480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401011"/>
    <dbReference type="eggNOG" id="ENOG502QV4T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DKZ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF4">
        <property type="entry name" value="OS02G0499000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="74"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ95765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="218" mass="24783" checksum="EC9FBE19BABCBE1B" modified="2011-05-31" version="1">MVSLRRRRLLGLCSGKDSLPVDLPKPVENEKNGEVEHANVNPLSVHPLPLTRTSDVLPESSNGSDSLKEEKNQYYPGKEIKRRKRHRRKQYVDQEPCIMRGVYFKNMKWQAAIKVDKKQIHLGTVGTQDEAARLYDRAAFMCGREPNFELSEEEKNELVKYTWDDFLAMTRNTITSKKQRKVGSLRHNKADLFIGDTEMVNGGGSSNSDDGDVDTSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2DR82</accession>
    <name>F2DR82_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123425252</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Flower</tissue>
            <tissue evidence="13">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AK366400">
        <property type="protein sequence ID" value="BAJ97603.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK374249">
        <property type="protein sequence ID" value="BAK05446.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964865.1">
        <property type="nucleotide sequence ID" value="XM_045108930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DR82"/>
    <dbReference type="SMR" id="F2DR82"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123910"/>
    </dbReference>
    <dbReference type="GeneID" id="123425252"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0102120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0102120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0102120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0123910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425252"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009845">
        <property type="term" value="P:seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd14662">
        <property type="entry name" value="STKc_SnRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000237">
        <property type="entry name" value="Serine/threonine-protein kinase SAPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000085">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF542">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ97603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK05446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="341" mass="38594" checksum="F560A77505408526" modified="2011-05-31" version="1">MERYEVIKDIGSGNFGVAKLVRDVRTKELFAVKFIERGHKIDENVQREIMNHRSLRHPNIVRFKEVVLTPTHLAIVMEYAAGGELFERICGSGKFSENEARFFFQQLLSGVSYCHSMQICHRDLKLENTLLDGSEAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLSRREYDGKVADVWSCGVTLYVMLVGAYPFEDPDEPKNFRKTITRILSVQYSVPDYVRISMECRHLLSRIFVANPEQRITIQEIKNHPWFLKNLPIEMTDEYQMSLHMVGVNAPPQSLEEIMAIIQEARIPGDGSKFAGQLSVPGLGSMELDDIDDVDADVEDSGDFVCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>F2DUQ9</accession>
    <name>F2DUQ9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123399003</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367627">
        <property type="protein sequence ID" value="BAJ98830.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371239">
        <property type="protein sequence ID" value="BAK02437.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949373.1">
        <property type="nucleotide sequence ID" value="XM_045093438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DUQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0461550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0461550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0461550"/>
    </dbReference>
    <dbReference type="GeneID" id="123399003"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0382050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0382050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0382050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0461550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0461550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0461550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399003"/>
    <dbReference type="OrthoDB" id="664446at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF19">
        <property type="entry name" value="OS11G0130700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="1">
        <location>
            <begin position="131"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ98830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0461550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="369" mass="41635" checksum="CB0F783CB9788895" modified="2011-05-31" version="1">MLRSLRNLDHVPRSAYQSWIMARNIMGVKDKKNWGKPNCLRHITEGISREEQRWGTKEVLFSHPADNVSSTFIFKNSSHRDGAIYKRSFALRKSCRVTDRDETQLEPMMLSEPADCFPDRERCCVHCKCPMMQIFTLKLASVSINTSPVELYGYIAVRDHLDLLLNYIVNRSRDDPIIAQQGSLIEMTGPKRCIAMDSSVLVEFDIRIKTGEKEEDDLTLIDGATDYCDLTTPSMSFTNRINGDCGAVDITLARVYNAVEATIDVLILKVQNGFNLSLSLFVFVGGSDQEIPLFHGTTAESCGLRRYVIAVEMDTWLQLKFKVGQNGSKNDHLERSCHFKANIHGCACQQIMLQHASLSLKVTWSTVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2E7J4</accession>
    <name>F2E7J4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC123426894</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AK372118">
        <property type="protein sequence ID" value="BAK03316.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966732.1">
        <property type="nucleotide sequence ID" value="XM_045110797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E7J4"/>
    <dbReference type="SMR" id="F2E7J4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0172990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0172990"/>
    </dbReference>
    <dbReference type="GeneID" id="123426894"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0142970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0142970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0142970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0172990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0172990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0172990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426894"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010262">
        <property type="term" value="P:somatic embryogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043303043" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="31"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="305"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BAK03316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="627" mass="69077" checksum="487C35212EC120EA" modified="2011-05-31" version="1">MAASPEMLRRCWWAAAAVLSLVLAVSRVAANTEGDALYSLRQSLKDANNVLQSWDPTLVNPCTWFHVTCNTDNSVIRVDLGNAQLSGALVSQLGQLKNLQYLELYSNNISGIIPLELGNLTNLVSLDLYLNKFTGGIPDTLGQLLKLRFLRLNNNSLSGQIPQSLTNISTLQVLDLSNNNLSGEVPSTGSFSLFTPISFGNNPNLCGPGTTKPCPGAPPFSPPPPFNPPTPVTNQGDSKTGAIAGGVAAGAALIFAVPAIGFALWRRRKPEEHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLTDGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASRLRERGPNEPALEWEKRTRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEDFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKVEMLVDPDLQSVYVEHEVEALIQVALLCTQGSPMDRPKMSEVVRMLEGDGLAERWEEWQKVEVVRQEAELAPRHNDWIVDSTFNLRAVELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2EB28</accession>
    <name>F2EB28_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123443711</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373353">
        <property type="protein sequence ID" value="BAK04550.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976145.1">
        <property type="nucleotide sequence ID" value="XM_045120210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EB28"/>
    <dbReference type="SMR" id="F2EB28"/>
    <dbReference type="FunCoup" id="F2EB28">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2EB28"/>
    <dbReference type="PaxDb" id="4513-MLOC_70397.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0266020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0266020"/>
    </dbReference>
    <dbReference type="GeneID" id="123443711"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0220590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0220590.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0220590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0266020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0266020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0266020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443711"/>
    <dbReference type="eggNOG" id="KOG3457">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133423_3_0_1"/>
    <dbReference type="OMA" id="KYTRSHH"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006398">
        <property type="entry name" value="Sec61_beta_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK04550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="83" mass="8368" checksum="9443A1A3D3A424D3" modified="2011-05-31" version="1">MVANGDAPARGSAAAAASLRRRRTTSGAAAAGGGAGASTMLQFYTDEAAGRKMSPNAVLIMSIGFIAVVAVLHVFGKLYRTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKN5</accession>
    <name>F2EKN5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123420662</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Seed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK376713">
        <property type="protein sequence ID" value="BAK07907.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044963346.1">
        <property type="nucleotide sequence ID" value="XM_045107411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKN5"/>
    <dbReference type="SMR" id="F2EKN5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092150"/>
    </dbReference>
    <dbReference type="GeneID" id="123420662"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0075870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0075870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0075870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0092150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123420662"/>
    <dbReference type="OrthoDB" id="1073427at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF85">
        <property type="entry name" value="OS05G0581900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730895" evidence="8">
        <location>
            <begin position="27"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="9">
        <location>
            <begin position="88"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK07907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="265" mass="26888" checksum="FFAD151D3188B49E" modified="2011-05-31" version="1">MGLGTVHHCLASLLVLLLLRCCHVGGVEQRERAAVVRLERARDMPERDVTTPLATVPLVNPTPMPEATAAPTLAHPTAAAGVGGGAGSWCVASPSAGAAALQVALDYACGQGADCSPIQPGGSCADPDTVRDHASYAFNSYYQKNPVQTSCDFAGAAILTSTDPSTTTCKYPSTSTGASVLNTTNPLTPVTPTYGSPPGGYYNSPPGPGGYYNSPPLYGSMSPPDYGGSISAATAMMPGSKSTTVACTSLTCLLVVATVSLNLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL34</accession>
    <name>F2EL34_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447169</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Seed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Proteoglycan that seems to be implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the classical AGP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376862">
        <property type="protein sequence ID" value="BAK08056.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979685.1">
        <property type="nucleotide sequence ID" value="XM_045123750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EL34"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406650"/>
    </dbReference>
    <dbReference type="GeneID" id="123447169"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0338510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0338510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0338510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0406650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447169"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044959">
        <property type="entry name" value="AGP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36321:SF2">
        <property type="entry name" value="CLASSICAL ARABINOGALACTAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36321">
        <property type="entry name" value="CLASSICAL ARABINOGALACTAN PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730900" evidence="13">
        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK08056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="123" mass="11332" checksum="8EDFF4BCE192B36E" modified="2011-05-31" version="1">MARFAVVAAIVALLAVTAAAQAPGAAPRMAPLPAPPARSPATAPAPVATPPTAASPSPMASPPAPPTDAPTDAPSAMTPSAVSGAPAGSPAGAPSGTPASSAVYTSTVSFVAVAGAVAAAILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL93</accession>
    <name>F2EL93_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123428881</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376921">
        <property type="protein sequence ID" value="BAK08115.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968928.1">
        <property type="nucleotide sequence ID" value="XM_045112993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EL93"/>
    <dbReference type="SMR" id="F2EL93"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0036390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0036390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0036390"/>
    </dbReference>
    <dbReference type="GeneID" id="123428881"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0028190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0028190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0028190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0036390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0036390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0036390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428881"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010287">
        <property type="term" value="C:plastoglobule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080177">
        <property type="term" value="P:plastoglobule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904965">
        <property type="term" value="P:regulation of vitamin E biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009644">
        <property type="term" value="P:response to high light intensity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080183">
        <property type="term" value="P:response to photooxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF120">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="211"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK08115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0036390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="706" mass="78470" checksum="D92D95EB2597C57B" modified="2011-05-31" version="1">MAASTSATAAVSFSLPSYPRRGLRRLRRRPSLRAASTAAPPSPDLSIQLSPRPSPRSSPPVVPSLARDRADDLQAESRAMTRAAAATVYTPELLASRYGSQPFQVALRAAEVLSKLGAFGLTLLLDQQRGESSSSAKRRARAVELRTILTRLGPTFVKIGQGLSTRPDLCPTEYLEELSELQDSLPTFPDEEAFACIEWELGFPLDSMYSAMSPSPIAAASLGQVYKARLKYSEQLVAVKVQRPGIEDAIGRDFYLLRGLGFLINKYVDIITTDAVALIDEFARRVFQELNYVQEGQNARRFKKLYADKQDILVPDIFWDYTSAKVLTMEWIEGVKLNQQAAIEGQGLKVLDLVNIGIQCSLRQLLEYGYFHADPHPGNILATPEGKLAFLDFGMMSETPEDARVAIIGHVVHMVNRDYEAMARDYYALDFLKPEVDVSPIVPALKNFFDDALDSTVSELNFKTIVDGLGAVLYQYPFNVPAYYALILRSLTVLEGLALYADPNFKVLAASYPYFAKRLLTDPNPYLRDALIELLFKDEKFRWNRLENLLVQGRQDREFVAKDALQPVLKLLLGPDGENLRVLVVKEAVRVTEAITFGTLIGSYNAAPEFLKPLISNGNPAGPFKISDVEREQMLELRDTVLRIWGLLRSSDTFDPSLLQPIVQVLQEREARVFGSRIAGGVSQRLAARLLQQLLRIPPAPVPGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>M0VZS8</accession>
    <name>M0VZS8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123447106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979611.1">
        <property type="nucleotide sequence ID" value="XM_045123676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VZS8"/>
    <dbReference type="FunCoup" id="M0VZS8">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0VZS8"/>
    <dbReference type="PaxDb" id="4513-MLOC_39004.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405050"/>
    </dbReference>
    <dbReference type="GeneID" id="123447106"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0337190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0337190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0337190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0405050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447106"/>
    <dbReference type="eggNOG" id="ENOG502R5Y8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0VZS8"/>
    <dbReference type="OMA" id="HAAFCHA"/>
    <dbReference type="OrthoDB" id="693773at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012876">
        <property type="entry name" value="DUF1677_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33108">
        <property type="entry name" value="OS01G0745000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33108:SF13">
        <property type="entry name" value="OS03G0180100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07911">
        <property type="entry name" value="DUF1677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="129" mass="12997" checksum="5B00613ABB235372" modified="2013-04-03" version="1">MSAVVVQDVAPPIEAASAAEVEWAACACCGLREECTAAYAAGVRAQFAGRWLCGLCGDAVGEELAGCGVGGGGSASATAEVEAAIARHAAFCRSSPAAAERLIAAVRRLLRSESGRKAKAVVVLEFQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0WB23</accession>
    <name>M0WB23_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 9A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123449090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982111.1">
        <property type="nucleotide sequence ID" value="XM_045126176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WB23"/>
    <dbReference type="SMR" id="M0WB23"/>
    <dbReference type="FunCoup" id="M0WB23">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WB23"/>
    <dbReference type="PaxDb" id="4513-MLOC_4715.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0390140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0390140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0390140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449090"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0324560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0324560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0324560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0390140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0390140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0390140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449090"/>
    <dbReference type="eggNOG" id="KOG2319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WB23"/>
    <dbReference type="OMA" id="FREYPYL"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WB23">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000325">
        <property type="term" value="C:plant-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.120:FF:000003">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.80:FF:000007">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="6">
        <location>
            <begin position="109"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="430"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0390140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="483" mass="53990" checksum="3F53D5DE60A4CB45" modified="2013-04-03" version="1">MEGGADAFGSSTAPLAWHDFLERMRQPSAAEFVKSIKGFIMNFSNRAPDPERDSAAVQEFLENMEGAFRAHTPWAGSSEEELESAGEGLEKYVMTKLYNRVFASVPEDVKSDEELFEKISLLQQFIRPENLDIKPEYQNETSWLLAQKELQKINMYKAPRDKLACILNCCKVINNLLMNASHMSHDNPPGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRCQSRLVSEAQYFFTNILSAESFVWNIEAESLSMDERDFQKKMDLARERLLGLSVGSDNQENQTNLDVGEHGSQTLKASGNSDANLPLKDHVQGPVQDMKRASDVSSKSVERVQSISDLEKKGATELVKDDDLSKIFQEYPFLFARAGDLTVADVDTLLNSYKQLVLKYVALSKGMGVTPETPLVQNMQTASDLQISEEPENVKSVVNSCDSTEASSKTQEDIRNEIPDSEEVGNAGTQQAAVDPSRDQKTLKNESSDQPEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0WDH4</accession>
    <name>M0WDH4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0WDH4"/>
    <dbReference type="SMR" id="M0WDH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0296310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0296310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0296310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0296310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0296310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0296310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WDH4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000069">
        <property type="entry name" value="Beta-glucosidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF167">
        <property type="entry name" value="4-HYDROXY-7-METHOXY-3-OXO-3,4-DIHYDRO-2H-1,4-BENZOXAZIN-2-YL GLUCOSIDEBETA-D-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0296310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="512" mass="57019" checksum="8349BB43C32FFE4A" modified="2013-04-03" version="1">MSLGAAAFFCLLLSLRVQDAAAADLGFTRSDFPREFVFGAGTSAYQYEGAVAEDGRSPSSWDIFTHAGSMPDKSTGDVAADGYHKYMEDVKLMSETGLEAYRFSISWSRLIPNGRGAVNPKGLEYYNNLIDELVNHGIQVHITLHHVDLPQILEDQYGGWLSPRIVEDFTAYADVCFREFGDRVASWTTMDEANIGVLGSYGNALFPPGRCSDPFGATKCTAGNSSIEPYIAANNTLMAHASVFSLYREKYQHKQKGIVGINIYSYWSYPLTNATVDLEATQRCKDFLYGWILEPLVFGDYPQVMKKNAGSRLPPFTKAQSELIKGSLDFIGINHYFSVYVNDHPLDTGVRDYTADMSVDLRRSRTDPPAGQGPPTNVPSDPKGLQLALEYLKETYGNLPIYVQENGIGSADDSLDDTDRIGYLSSYMESTLKAMRNGADVRGYFAWAFLDLFELLAGYQSRYGLYQVDFADERLPRQARLSARWYSGFLKHNGSSALVWRTRVNQALNLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>M0WTJ6</accession>
    <name>M0WTJ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979277.1">
        <property type="nucleotide sequence ID" value="XM_045123342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WTJ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0331500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0331500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0331500"/>
    </dbReference>
    <dbReference type="GeneID" id="123446799"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0276120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0276120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0276120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0331500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0331500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0331500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446799"/>
    <dbReference type="OrthoDB" id="676503at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WTJ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040420">
        <property type="entry name" value="At1g76660-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798:SF5">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0331500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="321" mass="33913" checksum="61AD445988F805E6" modified="2013-04-03" version="1">MQQAAEAEGSNSSVHTVNAAAVVLAAAARSSDGLRPQLHHLQQEQLHGHDSDSATKKRWWSRLSATLCFRPHHHGHPRRVIAAAYEDGDTPLPRAAAHTASASAYVHQAPPAQPVFAFAAPPSSPASSLFQSEAPSPVLLDLHGTAGSSPSMFAVGPYARGPQQLVSPPVLYSALTTEPSTAPRTPPATTGPSSPEVPFARFVHDGHELLFHHAAYQLRRQPAPPLASPGPEAGSPSSPSSPRLFRKLHRRNVQGSLLDGHVPVTSSCEGADAREQEDDDVPDSAGEFVFGNADGGVAAPEEGIDGKSWHFFPTAEHHAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>M0XEM7</accession>
    <name>M0XEM7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein CDI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123408968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044957919.1">
        <property type="nucleotide sequence ID" value="XM_045101984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XEM7"/>
    <dbReference type="FunCoup" id="M0XEM7">
        <property type="interactions" value="1222"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XEM7"/>
    <dbReference type="PaxDb" id="4513-MLOC_5940.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659410"/>
    </dbReference>
    <dbReference type="GeneID" id="123408968"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0547230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0547230.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0547230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0659410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408968"/>
    <dbReference type="eggNOG" id="ENOG502QPHU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XEM7"/>
    <dbReference type="OMA" id="FMDCDML"/>
    <dbReference type="OrthoDB" id="590152at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35105">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35105:SF2">
        <property type="entry name" value="PROTEIN CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33179" checksum="E7D86144942F22CD" modified="2013-04-03" version="1">MAFKGHHPGEHDNSHKLSSHASAPKMSPPAPCQLLGAESAHATAEPFRVYVGYDSREDIAYRVCRRSLLRRSSVPLEVIPIVQQELREAGLYWRERGPTESTEFSFTRFLTPHLAGYRGWALFVDCDFLFVADVAELARMADPRYAVLCVHHDYTPKEATKMDGAVQTAYPRKNWSSMVLFNCGHPKNRAALTPEAVSTQSGAHLHRFAWLDDADVGEVPFAWNFLVGHNRVDPADVGGTAPRAIHYTSGGPWFELYKDCEFADLWIQERDAYESEEKEGEVEGTRVPSSSAVAVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>M0YC26</accession>
    <name>M0YC26_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970743.1">
        <property type="nucleotide sequence ID" value="XM_045114808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YC26"/>
    <dbReference type="FunCoup" id="M0YC26">
        <property type="interactions" value="1448"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0YC26"/>
    <dbReference type="PaxDb" id="4513-MLOC_67751.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202540"/>
    </dbReference>
    <dbReference type="GeneID" id="123430985"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0168370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0168370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0168370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0202540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430985"/>
    <dbReference type="eggNOG" id="ENOG502S8AI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YC26"/>
    <dbReference type="OrthoDB" id="683235at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37225:SF1">
        <property type="entry name" value="OS04G0657900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37225">
        <property type="entry name" value="OSJNBA0011F23.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="66" mass="7453" checksum="F9BB4A11D51EF7C4" modified="2013-04-03" version="1">MDAGQFVIPILGIVGAAAATFYAVSFMEIREKSLEDLDDKYSEYEEGGGRQRRARRKSGRQAKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>M0Z3W3</accession>
    <name>M0Z3W3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123443265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975527.1">
        <property type="nucleotide sequence ID" value="XM_045119592.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975536.1">
        <property type="nucleotide sequence ID" value="XM_045119601.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975543.1">
        <property type="nucleotide sequence ID" value="XM_045119608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Z3W3"/>
    <dbReference type="FunCoup" id="M0Z3W3">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0Z3W3"/>
    <dbReference type="PaxDb" id="4513-MLOC_76550.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067260"/>
    </dbReference>
    <dbReference type="GeneID" id="123443265"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0053820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0053820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0053820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0067260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443265"/>
    <dbReference type="eggNOG" id="ENOG502R46J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Z3W3"/>
    <dbReference type="OrthoDB" id="593438at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Z3W3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF13">
        <property type="entry name" value="OS10G0134700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="57778" checksum="D5E9C146CA0D7CFC" modified="2013-04-03" version="1">MELAISAVTGELVSRFVSFLADKYRSSRCAQPEERLVKRLQQLLLRARTVVEEADGRYITNSGMLAQLDVLAGAMYRGYWVLAAFSYMSLEKTETTPLEEDAEVSCFSPTKRLRRVHGSARNNKAVYLADLQGMLESLEDVVASMTEFVVLLGGCDRMLPRPYDAYLYTGNIMFGRHTEKQKLLNFVLQHSPPGCTPAVLPVIGAPAVGKRTLVAHICKDERVRSHFTSILHLNGDSICRLADHDRSLLSGNTLVVVEVVSDVSEEDWAKFHSTLASMDSGSKVIIISRLRSSERLGTVKPIFLNTPSYEEFSYLFKTLTFRGANPVQHPRLAQIADQLAREFRTEWSLVAMNIFADLLGRNLDVHFWRGMLSRMRRSVERNLSMFGEHPRLLIERRHQVDVTDFVLHPNASPLCIMPSCTSGSSRTEVTTERASLPRVRLGDLMMDPGVRPQGDFNVVSWESRLPPYSSFIHFVPNGNGAPGLRKQSTPLTGRKRPFGRPFDLNDSCRDLVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-06-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>X5D8G6</accession>
    <name>X5D8G6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cleistogamy 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123428148</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2014-01" db="EMBL/GenBank/DDBJ databases">
            <title>An epiallele at cly1 affects the expression of cleistogamy in barley.</title>
            <authorList>
                <person name="Wang N."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KJ363931">
        <property type="protein sequence ID" value="AHW51118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968248.1">
        <property type="nucleotide sequence ID" value="XM_045112313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="X5D8G6"/>
    <dbReference type="SMR" id="X5D8G6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204770"/>
    </dbReference>
    <dbReference type="GeneID" id="123428148"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0170380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0170380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0170380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0204770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428148"/>
    <dbReference type="OrthoDB" id="207175at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060860">
        <property type="term" value="P:regulation of floral organ abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080050">
        <property type="term" value="P:regulation of seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097548">
        <property type="term" value="P:seed abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF142">
        <property type="entry name" value="FLORAL HOMEOTIC PROTEIN APETALA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="114"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="205"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="366"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="392"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHW51118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="487" mass="51804" checksum="7D77F829417C97BD" modified="2014-06-11" version="1">MWDLNDSPAAEGPPLSPSVDDSGASSSSAAAVVEIPDDAEDDSAEAVVTRQFFPPAAPGEGGPGNGNDRAAWLRLAGAPAPTVAAAAGGGTGGPAAASAAAKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGMEADINFSLEDYDDIKQMGNLTKEEFVHVLRRQSTGFPRGSSKYRGVTLHKCGRWEARMGQFLGKKYVYLGLFDTEEEAARSYDRAAIKCNGKDAVTNFDPSTYAEEFEPAASTGDAEQQNLDLSLGSSAGSNKRGSLDGGGMDDDGAAGSDQRVPMAFELDWHTAAARSTKAKFDQNSARHQMPPPALQLQAPHMQFSPRHHQFVGNADPGTAGGLSLTVGAGAGGGQWPPPPPPHHYQPPHPQQHHQQQQQRLQHGWGNVVPGTSWQPVQPPPPPHHQAGPVPNNAAAAAAAAAASSRFPPYIATQAQSWLQKNGFHSLARPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287EAW6</accession>
    <name>A0A287EAW6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287EAW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0671070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0671070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0671070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0556880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0556880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0556880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0671070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0671070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0671070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF7">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="57"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0671070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="157" mass="17697" checksum="B7EB20EFA1C730C2" modified="2017-11-22" version="1">MLRTEHISRKMKKENFLALVGCQVAVVAVVLAVSFIVKCFLQNVVPWKLKSRTPTELFSMMSSFPWLAILYTGIITTTFCLWAEIVAMRDVSATETAIIYGLEPVWGATFAWAIHGERWDITGLIGAVFILEPHLLCLGSDGIFQPKTRKTRLYSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287K4I9</accession>
    <name>A0A287K4I9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287K4I9"/>
    <dbReference type="SMR" id="A0A287K4I9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229820"/>
    </dbReference>
    <dbReference type="OMA" id="YLERTHD"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF51">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="2">
        <location>
            <begin position="62"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="476" mass="54098" checksum="99E9B732FA844DB9" modified="2017-11-22" version="1">MSRCKMHDLLRQLALNISKEECFIGDVETLRDGNMSKLRRITAVIKKDMLVLPRVDQVEVKVRTFLTVEGPWRSGDTLFKRFLLLRVLVLNYSLVQSIPDYIGKLIHLRLLNLDHTGISCLLESIGSLKNLQVLSLRWCNDLHNLPFAMTLLCSLRCLDLIGTNINQVPKGIGELKFLTYLRGYPIGDGSDNAVVQDGWMLEELSSLSQLRHLFLVNLDRAAHCSTDAVLTDKKHLKTLRLKWTGPGEGSYSEEDVSNAEEVFEQLIPPPNLEDLGIFRFFGQRYPTWFGTTYLYSLIYLNLIDVRSCVHLPPVWQLPNMKYLRIDGAHAVTKVGPEFVGCKKGDPVCNQLVAFPKLECLIFKDMPSWEEWSFFEEEVVADERGEDGAADIRKEDDPPARLRLLPRLVKLFLHGCPKLRDLPQQFGKDTACLKELSLIGLNNLKAVEDCPQLSEVLVIKDCEGLERIFNLPHVDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MYL4</accession>
    <name>A0A287MYL4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123447045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979541.1">
        <property type="nucleotide sequence ID" value="XM_045123606.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979542.1">
        <property type="nucleotide sequence ID" value="XM_045123607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287MYL4"/>
    <dbReference type="SMR" id="A0A287MYL4"/>
    <dbReference type="FunCoup" id="A0A287MYL4">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287MYL4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333990"/>
    </dbReference>
    <dbReference type="GeneID" id="123447045"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447045"/>
    <dbReference type="InParanoid" id="A0A287MYL4"/>
    <dbReference type="OMA" id="SELMACM"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287MYL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005539">
        <property type="entry name" value="ELK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03789">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01188">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="212"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="321" mass="35789" checksum="1F07B0DAD53AE789" modified="2017-11-22" version="1">MDQFPLLPSGSKANTSTPLYLALDNTTSGASTSPVPEPAPAPPEPSGHSNHDRGTDTVKAKIMSHPLYPALLTSFIECQKVGAPPEIVGRLSALAADLDSDPGDRRQDPVPDPELDEFMETYCDVLVRYKQELARPVQEADQFFRAMEAQMESFTLDDQSYEGGGSSEDEQETGDVGGLPEITSHCAEDKELKSHLLNKYSGYLSSLWKDLSKKKKKGKLPRDARQKLLHWWQLHYRWPYPSELEKAALAESTGLDAKQINNWFINQRKRHWKPTPPAMEYMPQQQNHHHPYGGASSSSPAPFRTTEGHYFAGGSVYPRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287PSJ5</accession>
    <name>A0A287PSJ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123447206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979724.1">
        <property type="nucleotide sequence ID" value="XM_045123789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287PSJ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407290"/>
    </dbReference>
    <dbReference type="GeneID" id="123447206"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287PSJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF322">
        <property type="entry name" value="OS03G0144300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="6">
        <location>
            <begin position="59"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="476" mass="54029" checksum="3BBC9BA84C52E5BE" modified="2017-11-22" version="1">MKGPDKRTSTPASAAFQSLRLVFVLATVLWASFFYYHFAVLNAGAMRAAVLDGASADPCRGRYVYVHDLPPRFNADILRDCQNISDHWPDMCGFVSNAGLGRALADPLDGDFTGENGWYGTHQFALDAIFHNRMRQYECLTSHSALANAVFVPFYAGFDFVRHHWGYDNATRDAASVDLTEWLMRRPEWARMGGRDHFLVAGRTGWDFRRSNNMNPSWGTDLLHMPGGREMSVLVLEVSLVPHSRDYAVPYPTYFHPRSDADVRRWQDRVRGLERRWLLAFVGAPRPDNPYNIRQQIIAQCEASDVCHQLGCAFGTSQCHSPGNIMRLFQRATFCLQPPGDSYTRRSAFDSMVAGCIPVFFHPVSAYLQYRWYLPKHHETYSVYIPEDDLRSRNVSIESVLRAIPPETVERMRDEVIKMIPRMVYADPRSKLETVKDAFDVAVEGIIDRVDIARAEAAAAESSFGHAMEMFGFGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W3J0</accession>
    <name>A0A8I6W3J0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gliadin/glutenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6W3J0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001070.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0001000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0001000.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0001000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001070.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001954">
        <property type="entry name" value="Glia_glutenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33454:SF18">
        <property type="entry name" value="GLUTENIN, LOW MOLECULAR WEIGHT SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33454">
        <property type="entry name" value="PROLAMIN PPROL 14P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13016">
        <property type="entry name" value="Gliadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00208">
        <property type="entry name" value="GLIADGLUTEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00209">
        <property type="entry name" value="GLIADIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="2" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="4">
        <location>
            <begin position="3"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001070.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="138" mass="15566" checksum="1E6DD0908DC0A2FF" modified="2022-05-25" version="1">MSQRIARSQMLEQSSCHVLQQQCCQQLPQIPEQFCHEAVCAIVYSIVLQEQPQRLVQGVSQPQQQSQVQQVGQCSFQQPQPQQGQQQQVPQSVFLQPHQLAQLEATVSIALRTLPMMCSVNVPLYRILPFGIDTRVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGJ2</accession>
    <name>A0A8I6WGJ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123424601</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964178.1">
        <property type="nucleotide sequence ID" value="XM_045108243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGJ2"/>
    <dbReference type="SMR" id="A0A8I6WGJ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0108190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0108190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0108190"/>
    </dbReference>
    <dbReference type="GeneID" id="123424601"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0088780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0088780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0088780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0108190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0108190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0108190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424601"/>
    <dbReference type="OrthoDB" id="1932925at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF327">
        <property type="entry name" value="XYLAN O-ACETYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="171"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="227"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="93"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0108190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="59330" checksum="892EF88026ADF252" modified="2022-05-25" version="1">MQQRRKSPFAAAPAAAKQGEGEEAPARRPGAPLSLAGLLVSIFLVALFVYNEDSAVVKAPLAALDAGAGDFPLAGASRARSAPDLHLLHEVNPRSQQQQQGGEGDEEERGHVEQVESRRKATDSDRADAAPARADKRAAPATTTTAPPSPSSGGNTSSSGAAVVAQPEPPACNLYQGWWTYDAEGSQVPLYREPECEFLTEQVTCMRNGRRDDSYQRWRWQPSSCDLPRFDAGALLERLRNKRLMFVGDSLNRNQWESMVCLVSSAIPSRDKKSLAKFVGPNGSMNVFRAAEYNATVEFYWAPFLVSSNSDDPQAHSVADRVVAWRSIAKHARHWRAAHFLVFNTYIWWLNNFQMKVLKNPRALPDKYTLVDRPVAYKEVLKTWAKWVDRHVDPDHTRVFFMGMSPNHGVPEAWGGGPGAVKCAMETQPILNHTGPLYIGTDWRLHGAAEAVLRSMRRVPVHLVDITALSEFRKDAHTSVHTLRQGKLLTAEQQADPRTYADCIHWCLPGLPDTWNHFLYAQIAAPSPPAQEASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WHT2</accession>
    <name>A0A8I6WHT2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123410072</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044958899.1">
        <property type="nucleotide sequence ID" value="XM_045102964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WHT2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0046890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0046890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0046890"/>
    </dbReference>
    <dbReference type="GeneID" id="123410072"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0036550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0036550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0036550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0046890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0046890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0046890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410072"/>
    <dbReference type="OMA" id="NCVALQY"/>
    <dbReference type="OrthoDB" id="695336at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF241">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="82"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0046890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="263" mass="26613" checksum="D2694771F90D0C5A" modified="2022-05-25" version="1">MASPFLPGSSSSSASSPLSYLIPRQPPPSSVVAQGFSCGALVQQAGLYGGVGVSAAGGVEAVEVRQGGRHAGHPPLPRPPPRQCPRCRSANTKFCYYNNYSRKQPRYFCRACRRHWTEGGTLRDVPVGGGRKNRRNGGGGAKGGAKAPSTVTAEVSTAATTAIQGSGAGALAGAVDAFIPADILRQMLSQSASFTASGGGGGYGIDLSAWQQMTGAAAAPQGGSDVGEVGGTAPAADANCGTGAQYWSGWNLQDDMPGFDGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WII5</accession>
    <name>A0A8I6WII5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WII5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0200120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0200120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0200120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0200120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0200120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0200120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF106">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="355"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0200120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="686" mass="74628" checksum="3B0437912E30595E" modified="2022-05-25" version="1">MATAEAQGDGAPRRAGYRCVAPDIRGTGGTEASADVASYTAFHVVDNAIALLNAPGIHKVHIKTVSDLETGRLGEARRRPTPRGELGLWGGVTDRVSWAGAAAAWPTGAGVAVGGATIRWSTRGAGAEALTASVPSPDARRRLSLEGFHLVRAGRLGTRGGRSSFWRRESIEAAGTRCQGGSGTRDPIWIPRSREGAALALGPHPDRIEREGRCSRGGLAAICRCEIRDLSRLPATKSATRVGAMPPSANRKRRRRVSSSSAAAPAATSESSDAYSSWGSLPGDLVCLIGWRALAGDLRDYVRLRAVCRDWRAATISPGGRGLHDPRFHPRRWRMLPEGHGLHPGHTKLRGYVRFINLSTGAIVRVRLPLFSNHCVLDSVDGILLLQRDEDTVVRLLHPFTGDIVDLPPLATLLRGLAPGQDKMFSWNVLRTVGATSFTVSADGVVTVMIGLFKLGRVACATTMDQQWRLSAWRISNGWRPLSFQGKMYTLHTPTNGSELQIFQIDLPGPGEKEDFDTVLCHLPAPKLIATCLAGKPRRPFYHLAECDSEILVIGRDLSSSLDVEVYRLSDIILDRIAPVTSIGGNALFVEERVLSVSSRVHPTIVGDSVVHLHNKEIYLGHYQLGTGTWLPTADGCVRRDVPSPCSLIYHIFTCCYRPTWNKGGLLYQGNVQPSWRVKRKWREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WK66</accession>
    <name>A0A8I6WK66_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WK66"/>
    <dbReference type="SMR" id="A0A8I6WK66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0150720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0150720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0150720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0150720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0150720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0150720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0150720.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="19391" checksum="1A8B17E98A8ECB23" modified="2022-05-25" version="1">MSSSRFIYPSSQPNGHCPLHHHGRKPNRASHPVEKAANREEEEDDRARPVEMYQAIPYSAGRLPAWPRRSEQAEAEGAGGQEGASARGDGGEAVRQAVAESPVLVVGRRGCCLTHVVKRLLQGLGVNLAVYEVAAAVTGDEAVVALPAVLVGGRLLGGLDRLMAVHISGELVPILKDAGALWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLX3</accession>
    <name>A0A8I6WLX3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLX3"/>
    <dbReference type="SMR" id="A0A8I6WLX3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089930.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0089930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0089930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0089930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15797" checksum="55B42205F2E90B5F" modified="2022-05-25" version="1">MVLVSAPVGPRPVYDSDVEVCEPEISTHLEMLLEKLTLVEKVNKYLKEKLKIIEDKNMEMELHVAEVIDDRKMKIDAMRLKMDAMRLKMDEMHLKIRNIRKYAIDKEAWYHYVVGSIVTLLVILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM76</accession>
    <name>A0A8I6WM76_HORVV</name>
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        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase ATM</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123442347</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044974292.1">
        <property type="nucleotide sequence ID" value="XM_045118357.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WM76"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218410"/>
    </dbReference>
    <dbReference type="GeneID" id="123442347"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442347"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000015">
        <property type="entry name" value="Serine/threonine-protein kinase ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000023">
        <property type="entry name" value="Serine/threonine-protein kinase ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057445">
        <property type="entry name" value="ATM_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25360">
        <property type="entry name" value="TPR_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="1902"/>
            <end position="2555"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="2663"/>
            <end position="2975"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="18">
        <location>
            <begin position="2987"/>
            <end position="3019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1856"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2417"/>
            <end position="2444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1856"/>
            <end position="1866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="3019" mass="339645" checksum="A1796502C6CFDE20" modified="2022-05-25" version="1">MATALDVEVVVQKLASDRARPRDEGVKLLGTWLQGDRAPTFCRLLARNTATATARAKPGQLASGATWPFLITALAKCVLADMATKRRGATRSAAAGMLRAAVRCAEDARLSGHSLLLISVAKQLFSHILEVIKDAPSFQLEYSPILRQLLTVNEYRYQMKPRTYSNLVVLYMKKVATGFDANFSNQASSKEESFRCTWTLHALLENPPGDYPDTMRDEVVNGFCAIFSHIRREDGKLTSKLMECVNTFLLMDGPNLGDKSVEIHNAVQDFILRSWLMTRDLRLKSLFIAYAKIQLKLARAIPKVLERKNLLGVIMDDLDQNVNTEAGLLWCEASRDAKDVSLRCSLEELMDLSATVFYQAYKSPAPMRGDDEKKLKTEDVISRITNSLKKGSLCWIGTVCLLVHKYGYRVDKTLLISWLQACCQSLKSALSKANVIRSQDSLLWIIRALKEFSAMLIVNTREESHCLLTKSEMSTVEGYWQDIWNALIHALPLFSPTALVADSALNLLGGMIMRDQVRTSVVSEDTWNLQTFKQSPSSSALYFIACYFSRIGLQGDLSNASLVRKNLLRSTLELIQSVSKGSSLLNEKNVVMIPEAIFSICAGFSSSAINSADASQLFDECKDFSKLLLEGENWVIKDELGYSVEALSEISTESSSKVISDKCNRAHLPGHIQQQLLDQLMEFKEFMASNEQLEKADLCTLVYRCSLLCNLIHCALLSRAIEEKSLFLKELFGYVTGIIKYMISMVMKKHEELSHGLTSVGSAFETAGSILSSFQTFLSAPIFRLRSVSNRASSELIKGVTKLLDELLVEFSQLFSCLSSSVNTSDSENTSKMFPISSVNLSEDLNPFVGHKSVVDMDFDMADSGEVDSITASVSGSIGISSRLSEWKLELVGVISTFFSVSAPHTWEILYNLVEKESDTKVSQAILLNLCQNISASSKSLSSVVHLIFNMRDKCASLLLGSADCLTHLHALLRTLMATRDVGQNTDGKPQAYNEVSNENQDILLDLVTKGTEISITDWFFRIKLIDCISHFIHLFPDGAQDMIGHLLNMLHDTDYRVRLYLARKMVVLFQTWEGHDELFRDVCSNIGVKMVQFSSEIPVKSREVLAVGPQSVPVIETVLITLAHLSVYSEEVEFECAFMISAVAAIQPFERKLEYALFDSISRKLCYASRSKYLDQLMGPILFRWVACEVSLVSLVKVQEMFGFDTAKPKEFIEHICPWLLSFLILRGDAAGLNWISKTLLQPLSAVIKTYFVQIFGLCIAAKNGTGPEKDLAETVLYESLLQLGEISEFERDGLIRKHMVSIVGVLLTVSSTARQSELPYFSREILAHTIKQVVDGFVETADDDSADTVVIDKVNIFRADRVFKFLLAIHQQVTEAGHPRHMSHRLCAIEVLIDVLGHRVVHYSTCFYIICILGNYIWRQTLQGQCCNILSKLLAAFNANSSTETVAVLGTQLQVLVPKLITCCLPNDREGGRPHGDLSKVLSLLHQLTVDADPLLYDYIRDLEPLPGVDCLKDIKVFHASLSDSYASRDQFLKFVQRAPHLPPELFLLSLRTHHKKLLSGEIICRGDVSAGHADTVSCWGSDPDVVSAVWTLVDFCSSSSVANEASSVLADFISRAGISDAHQVIFDVPNLTQKHSLQLQSGSTSKEDKMCSDYGISDDILLELLKLLKTYLSDESVEIIDVSSRTLKGILSTSKGLNSLQRLDSLDRSLLMVHSRGINTQLVEQTLLGMDKYSTVSLEDSDIWQTDGRPYKQWLCTLVSSLISHCDDIILRLCRSLVFLKVEAAELLLASALVNIAGNMDSISSICGLISSKVEQIIFCDSNHLMKSVKLFLDALNVVRSFYVTERARASPYNTRASPSPYNTPKDGSRSARSKSRSPAATPSSSWKKVYWLSVDYLVAARAAQRCSCDFATLMYVELWCEEQFNKLELGPPDFSQQESLPPHVGLLVDAFTHINEPDSIYGITLANEVASQIIRYEHEGNWSSALEYYDLLVRSTPKENLADFAGTVLTGPSASSKAEGNLLNWKMHKGLMRSLQKTGCSHVLDIYSQGLTNQKTCLQQDSEFIDIQYEAAWRAGNWDFSFFVPYSSQPSSRSRDYSLFNENLHSCLRALQNGDSEKFQGKLSHSKMDLVLDLSNASKESTKYIHSTVLKLQMLDHLSMAWELRWKFCPDQAPKSNVGTEEFSLVPAVPKRNQLELLSKEWNFILCQTERNLDLFEPFLAFRRGLLKILGSEEHLIEHLFQSASALRKGLRFSLAAASLYELKELCCHRDQQTVPSTYFLSKLEEAKLLRAQGQHDMAIGLGKYILKNHTDERDKSDVYRLVGKWLAETRSSNSRTIIEDYLNHSVALDKKYMSRQCRTHFHLAHYTYNLFKSYEERLSSNEWQAALRLRKYKTKELDTLLKRLKSSSKAEKSDYGAKIQELQKQLALDREEAQKIQDDRDEFLSLALEGYQRSLVVGGKYDLQVVFRLVSLWFNLFSRKQVVDSMIKTTKEVQTYKFIPLVYQIASRLGSSKDAQGSTNFQNALASLLKKMAIDHPYHTICQLLALANGDRVKDKQRSRSSFVVDMDKKLAAENLLKELSSFHGALIRQMKQMVEIYIRLAELETRKEDTNKKISLPREFRSICQLELAKVPVVTATIPVDPNCRYEEGTFPHFSGLVDSITIMNGINAPKVIQCIGSDGNRYRQLAKSGNDDLRQDAVMEQFFSLVNMFLQNHRDTSERRLRIRTYNVVPFTPSAGVVEWVNRTIPLGDYLLDSNRIGGAHARYGTGDWTFMQCREHLACEKDKRKAFFKICDNFRPVMHHFFIERFLQPADWFQSRLAYTRSVAASSMVGYIVGLGDRHSMNILIDEDTAEVVHIDLGVAFEQGLMLKTPERVPFRLTRDIIDGMGVTGTEGVFKRCCEKTLSVMRENKEALLTIIEVFIHDPLYKWALSPLKALQRQKETEYNDSCLDDSQEACEGNKDAARAILRVKQKLDGYEDGEMRSVEGQVQQLIQDAVDADRLCQMFPGWAPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQQ9</accession>
    <name>A0A8I6WQQ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440468</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972973.1">
        <property type="nucleotide sequence ID" value="XM_045117038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0060300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0060300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0060300"/>
    </dbReference>
    <dbReference type="GeneID" id="123440468"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0047850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0047850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0047850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0060300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0060300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0060300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440468"/>
    <dbReference type="OMA" id="IKLNAMQ"/>
    <dbReference type="OrthoDB" id="285729at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019002">
        <property type="entry name" value="Ribosome_biogenesis_Nop16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243">
        <property type="entry name" value="HSPC111 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09420">
        <property type="entry name" value="Nop16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0060300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="21114" checksum="28842B5BCA4496B4" modified="2022-05-25" version="1">MGGSRRKFKRTRTKVRVGLPRKKPREFKPAFDLPEALAAAAAAEAGGRVPSWDAEGSVVKNYSAFGVVANPNLLGAHARGTRGLVQSASLQAPDMDALRAPVDEFGPVDTGSDLECDDLKSALGKKRRDGKSAPLEPLTKIQRLCIGRLIEKYGDNYKAMFMDTKLNSMQHSVGTLKKLCERYHVGGKAILYPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WRZ3</accession>
    <name>A0A8I6WRZ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta-amylase</fullName>
            <ecNumber evidence="3 10">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123409305</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="3 10"/>
    <dbReference type="RefSeq" id="XP_044958181.1">
        <property type="nucleotide sequence ID" value="XM_045102246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WRZ3"/>
    <dbReference type="SMR" id="A0A8I6WRZ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0040950"/>
    </dbReference>
    <dbReference type="GeneID" id="123409305"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0031800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0031800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0031800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0040950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0040950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409305"/>
    <dbReference type="OrthoDB" id="1660156at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005983">
        <property type="term" value="P:starch catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018238">
        <property type="entry name" value="Glyco_hydro_14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001371">
        <property type="entry name" value="Glyco_hydro_14B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF31">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00842">
        <property type="entry name" value="GLHYDLASE14B"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00506">
        <property type="entry name" value="BETA_AMYLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="450"/>
            <end position="451"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="538" mass="59044" checksum="734132AE7BCBF4B1" modified="2022-05-25" version="1">MAFNLAQSGAAAASFAPSTARRVAAPDSVTAQRNAYQMPSLRLQRREACKPSVAPSRPAMPMACRAHGSKARRSGVPVFVMMPLDTVNKCGSGLNRRKAMAASLAALKSAGVEGIMVDVWWGTVESEGPGKYNFAGYMELMKLARKTGLKVQAVMSFHQCGGNVGDSVNVPLPRWVTEEMDKDQDLTYTDQWGRRNYEYVSLGCDAMPVLKGRTPVQCYTDFMRAFRDHFADFLGNTIVEIQVGLGPAGELRFPSYPESNGTWRFPGIGAFQCYDRYMLSSLKAAAEAAGKPEWGFTGPTDAGGYLSWPEDTLFFRQDGGGWDSQYGQFFMSWYSQMLQDHGDRVLSGATSVFGSTPDVHLSAKVAGIHWHYGTRSHAPELTAGYYNTRHHDGYQHIARMLARHGAVLNFTCVEMRDHEQPQDAQCMPEGLVRQVAAAARAAGIGLAGENALPRYDDTAHDQVVGTAAERAEEDRMVAFTYLRMGPDLFQPDNWRRFAAFVKRMSKSGSCREAAEREAHGVAHATGSLVHEAATALRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTG9</accession>
    <name>A0A8I6WTG9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTG9"/>
    <dbReference type="SMR" id="A0A8I6WTG9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0050410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0050410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0050410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0039380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0039380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0039380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0050410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0050410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0050410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF8">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="5">
        <location>
            <begin position="26"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0050410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="129" mass="14560" checksum="9A21E14BA9A9E3B8" modified="2022-05-25" version="1">MSSSTSASRPSWTQYGSLPMDRCPDCPCSAPVIQLTSKEVKNGNYGREFMKCESKRERQIVKKSTHFEWLDDYVKRIQFNGAPTRELNLPSATTNLVSESAALTVGDADLRGEIKKMNKNLKQMIQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTL4</accession>
    <name>A0A8I6WTL4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123410755</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transcription factor probably involved in vascular development and shoot tissue organization. Binds to the DNA sequence 5'-CCGAGTGTGCCCCTGG-3' present in the promoter region Box II of the phloem-specific rice tungro bacilliform virus (RTBV) promoter. May regulate tissue-specific expression of the RTBV promoter and virus replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959634.1">
        <property type="nucleotide sequence ID" value="XM_045103699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTL4"/>
    <dbReference type="SMR" id="A0A8I6WTL4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0051920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0051920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0051920"/>
    </dbReference>
    <dbReference type="GeneID" id="123410755"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0040670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0040670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0040670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0051920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0051920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0051920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410755"/>
    <dbReference type="OrthoDB" id="1435597at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000009">
        <property type="entry name" value="probable transcription factor PosF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690:SF78">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690">
        <property type="entry name" value="TRANSCRIPTION FACTOR POSF21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="235"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0051920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="41044" checksum="25C5FAC2C4884643" modified="2022-05-25" version="1">MGTTNELMHRHVQAAVPPLPTPGRGMAPGQGQGGRQRAGLPPTPPSGAGSHSLHVADACMRMQDDSAPRKAHRRSRSDVPFGYFPPPSPKTESGWGLPGGGAVGGDELFNAFMSMEGMDGLNSSDGDSRGSSMPPANGADSSENESEDYGGVESQVFLWGEAGKKRNADGEPTAAAAARHARSLSMDSLMGKLSFSANGGEPSKFSLEFGSGEFTPAEMKRIMADEKLAEMALADPKRVKRVLANRQSAARSKERRMRYIAELEHKVQILQTEATTLSAQLTHLQRDSSGLATHNNELKFRLQAMEQQAQLRDALNEALTGEVQRLKQSATSERGGDAASSGGLAQQMQLRCQNQMLEMHKQQQIPFYQLEQPEQTRESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WW39</accession>
    <name>A0A8I6WW39_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WW39"/>
    <dbReference type="SMR" id="A0A8I6WW39"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0079520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0079520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0079520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0096650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000479">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000177">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF42">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="770"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="833"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="862"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1121" mass="123936" checksum="8C558F231DC01E71" modified="2022-05-25" version="1">MCGFTITGVHLRASSYVPQLATPAMRLTMEASSLLMLVSLTLLIDSIHQAKAVPFLQEQAGALLAWKATLQSHPAQLQSWTRGNNTSTWPCSWYGIKCSTHQTSHQEVITEISLRGLRLRGELDALNFTALATLTSIQLSHNRLTGRIPPSIASLGDLRFLILRRNQIRGPLSPALASLKNLRCLMFQENELSGEIPRQIGELENLVTLNLTANHLSGPIPSELGYLKKLVRLDFLNNNLIGPIPRNLGNLTKLTILYLSGNYLSGYVPRELGYLVNLRELFLEVNELMGPIPDTFGSLISLTRLHLWYNQLSGRIPQELGYLVNLEELVLSYNNFIGSIPSTFGRLINLSSLSMLNNTLSGCIPRELGYLVNLEQLDLGNNKLMCSIPNTFQNLINLTGLYVGGNQLIGPIPNIFGTLTKLTTLYLNDNQFSGHVPREIGNLNNLENLQLDGNNLSGPLPPGLCSGGRLKNFTAYDNNLSGPLPSSLVQCRSLVRVRLERNQIKGDISELGIHPNLLYMDLSSNKLFGKLSSHWRASGNLTKLCLSSNNLTGEIPTSMGQLPRLGVLDLSLNNLEGDIPSEMGNLNFLFQLNLASNLLHGSIPQEIWALSSLELLDLSSNNLSGMVHESIGNCLKLHSLNLSRNNLKGSIPTTLGLLQNLQYMLDLSDNSFVGAIPSQLSGLVMLDTLNISHNELNGSIPQSFQSMESLISIDVSYNELEGPVPGSRIFQEAQIQWFIHNKMLCGVVKGLPPCSSGATRSKEKRKTYNALVLGIVPSLISLVIVVVILIVRHEMKKSVAINTTNVTQENLFSIWSFDGADVFKQIVEATNDFSNIHCIGTGGYGSVYKARLATSEIFAVKKIHMIKDECRVNEAVFNREIKALVQIRHRNIIKLFGYCSCSQGRFLIYEYMERGDLAGILTNNGRAVELNWRTRTHIVLDVFRALAYMHHDCSSPIVHRDITSKNILLDPEFRACISDFGMAKILNTGGQNLTRLAGTKGYIAPELAYTDNVTEKCDVYSLGVLVLEIFMGSHPGDFISSLSLGTKNNDVSMKDLLDSRLVLPDTETTRKIYCMLTVAVQCLEPNPSRRPTARRANDEICAIKSCEGHVDYLHDLLKIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYS2</accession>
    <name>A0A8I6WYS2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123427496</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967499.1">
        <property type="nucleotide sequence ID" value="XM_045111564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYS2"/>
    <dbReference type="SMR" id="A0A8I6WYS2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0187910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0187910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0187910"/>
    </dbReference>
    <dbReference type="GeneID" id="123427496"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0155600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0155600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0155600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0187910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0187910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0187910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427496"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RKD-like"/>
    </dbReference>
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        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373">
        <property type="entry name" value="PROTEIN RKD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373:SF12">
        <property type="entry name" value="PROTEIN RKD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035207198" description="RWP-RK domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="RWP-RK" evidence="9">
        <location>
            <begin position="187"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0187910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="337" mass="38141" checksum="E97D7D2629602E74" modified="2022-05-25" version="1">MEGPAAAAAAVSTLSALAVFVSTVDRGAVRSAHGYRVAGKGGGCGWERWVEREFAFTPTSAREVPLPNDAQRLLPADWRGRPVYREGQIVGPWRCILAFDFVAAVAPPPVPPPLLCSFRNPRLMCVPSLYNDLTKVFQFQKLEEKVPELVQCDSDEKMTTSDAKDRVSVKQEAGSDTDEHPQFGEDLLASAEKQRRAHREYIASITLNDISQYFHLPIREASRSLKIGLSILKKKCREYGIPRWPHRKLKSLDALINDLEFVIDEETERDGVKQEDHGENEKENQDAIKALAKRKRMLETEKATIQQKPTLDLMDETKQFRQYVFKRKFRAKTLVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZA4</accession>
    <name>A0A8I6WZA4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123428213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968332.1">
        <property type="nucleotide sequence ID" value="XM_045112397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZA4"/>
    <dbReference type="SMR" id="A0A8I6WZA4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206690"/>
    </dbReference>
    <dbReference type="GeneID" id="123428213"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0171730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0171730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0171730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0206690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206690"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PPR_domain-containing"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47937:SF2">
        <property type="entry name" value="PENTATRICOPEPTIDE (PPR) REPEAT-CONTAINING PROTEIN, PF01535'-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47937">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE CHROMOSOME 2-LIKE PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
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        <location>
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            <end position="417"/>
        </location>
    </feature>
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        <location>
            <begin position="418"/>
            <end position="448"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="491"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="93"/>
        </location>
    </feature>
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        <location>
            <begin position="547"/>
            <end position="1006"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
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        <location>
            <begin position="574"/>
            <end position="584"/>
        </location>
    </feature>
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            <end position="638"/>
        </location>
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        <location>
            <begin position="642"/>
            <end position="659"/>
        </location>
    </feature>
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        <location>
            <begin position="665"/>
            <end position="708"/>
        </location>
    </feature>
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        <location>
            <begin position="720"/>
            <end position="743"/>
        </location>
    </feature>
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        <location>
            <begin position="744"/>
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        </location>
    </feature>
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        <location>
            <begin position="781"/>
            <end position="838"/>
        </location>
    </feature>
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        <location>
            <begin position="844"/>
            <end position="860"/>
        </location>
    </feature>
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            <begin position="865"/>
            <end position="878"/>
        </location>
    </feature>
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        <location>
            <begin position="885"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="949"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="981"/>
            <end position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1006" mass="110832" checksum="F27B0F03CDE20897" modified="2022-05-25" version="1">MAAPYRRLLLLRRFSISISQSHLAPHPQPAPLFPLARHFAFSSAEEAAAERRRRKRRLRIEPPLHALRRDPSTPPPPRDPNAPRLPDTTSSLVGPRLSLHNRVQALIRSGDLDGAAAASRAAVSSRVRPTVFTCNAVAASMVRAGRPDDAVALFAFFFRRSNIVPNVVSYNTLILAHCDAARVDDALQAYQEMLDGVTSFSPSSVSYRHLTKGLVAAGRVQDALDLLRDMLNRGQGADSIVYKNLIDGYVALDDWDKAFELFDELREKATVYDGVVHTSFMEGYWKKGMDKEAMDSYSSLLDRKFKMTPATCNVLLQTLFSHDKHKEAHDLWETMIDNHSPPSFIGVNSESYTVMVNQCFKEGRFQDAIEVFHRQPTKNVQMDVACFNNIIGKLCENGMLAEAEQLFQEMESKSVLPDVYTYTYLVDSCFKDGRVDDTMQYFHKMADGREHGPKFNIAFFNRMFQGLTEAGRIDDALKVYGRMSDKEIKPSTGTFEILVKASCKEGELDRARDLVRDMARGGVAAPPEFRECIVEIFRTADRQEEIEQAFEEKPVPPPPPPRPEYRPRSVPGFASNQTPGSYAPNQGKPGYGSPQPFHHGNAVPQILRPEGMSSEPRQTVFGNQQVQKTEVGAPNTSEYGLPSPHGTPPGTSQPQGQSPEFGTSRPWQQAAGASQVQQPKFSSDSPVQSGFGTPQPQQSTHVAHQTQHAGFGTPRPWQTGYGSQQGQQPGYGSQQAQQPGYGSHQTLQPGYGSHQAQQPGYGSHQAQQPGYGSHQAQQPRYGSQQVQQLGYGSQQVQQPGYGSQQAQQPRYGSQQAQQPGYGSQQVQQQGYVSHQGQQPGYVSHQAQQPGYGSHQAQQPGYGSHQAQQPAYSYQAQQPRYGAHQAEQTGYGSYQARQPGYGAPQPSRPPFSLPEAPQSGVGSNRSLPSHGHIKNQHDQFGSLPQGGQKVETQPGSGIAQSLPNNGHIGNQHDQFGSGGIKFSTQSPQEDFGTQASDEVAVKYAERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZB3</accession>
    <name>A0A8I6WZB3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123439494</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972140.1">
        <property type="nucleotide sequence ID" value="XM_045116205.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972142.1">
        <property type="nucleotide sequence ID" value="XM_045116207.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972143.1">
        <property type="nucleotide sequence ID" value="XM_045116208.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972144.1">
        <property type="nucleotide sequence ID" value="XM_045116209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZB3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0237320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0237320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0237320"/>
    </dbReference>
    <dbReference type="GeneID" id="123439494"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0197000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0197000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0197000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0237320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0237320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0237320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439494"/>
    <dbReference type="OrthoDB" id="681107at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482">
        <property type="entry name" value="OS11G0632001 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482:SF23">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="2">
        <location>
            <begin position="222"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0237320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="449" mass="50979" checksum="E98EC1AA710E06C5" modified="2022-05-25" version="1">MEAAPAGMHPQPESLTQILLSFSSPCEDTNLGHPQEDEEEQVGDVDSEAFGSKGGSWVRAENRLQPHQTTTYPSPCLDDEPDPQNGTINGDTELTHFFSDTETQGEIQDFEMCSTTPSWVGKNRSGNERCDSPLVCIQNTPTLFKWTEATTANLICVQKSGQEQCTWKRRIRLGKIPDQREPKMGRACALEKAMRSYVDQKSGQVVAPEIGREFDSLAEAFEFYNLYSWEIGFGIRYGQSRTNAQKSRTVQDIVCGCAGRPKKENTNSVACNCQALIRLHRTNDNGWYIHDFRSEHNHHLSSSCGEKLHWPSHRNIDTNTKDIVKHLMSNNIGLTKVFCVIASFFGSMENVPFNKRSLKYMCTRMNQETADDDIRKTIDLFSELKKNDPMFSDSVLVDSDSKIQALMWTNGKSRYQYKMFGDAITFDTTYRTNQYDMPFGLFVGVNHHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0P6</accession>
    <name>A0A8I6X0P6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glycosyltransferases</fullName>
            <ecNumber evidence="13">2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123424424</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of glucuronoxylan hemicellulose in secondary cell walls.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044963974.1">
        <property type="nucleotide sequence ID" value="XM_045108039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0P6"/>
    <dbReference type="SMR" id="A0A8I6X0P6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0100680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0100680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0100680"/>
    </dbReference>
    <dbReference type="GeneID" id="123424424"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0083040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0083040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0083040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0100680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0100680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0100680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424424"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010584">
        <property type="term" value="P:pollen exine formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000064">
        <property type="entry name" value="Glycosyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF33">
        <property type="entry name" value="GLUCURONOSYLTRANSFERASE OS04G0103100-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="92"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="12">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0100680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="392" mass="43633" checksum="DDF66FA90B2BAAC3" modified="2022-05-25" version="1">MASIRRPHSPAKAQHLLRHHHPFSTASPPSSPLRHASSASSSSSPRKSGYPHPFLFFTRRPLPRFAAFFLLGSFIGLLHFLSHLPLHPHLPAHPSSSHLNHLQQQQQQEQGQPITQQRLPDAAGSNAEEHSGQGKLLIVVTPTRARASQAYYLSRMGQTLRLVRPPVLWVVVEAGKPTPEAALALRRTAVMHRYVGCCDALNASASPAVDFRPHQLNAGLEVVENHRLDGVVYFADEEGVYSLPLFDRLRQIRRFGTWPVPTISDGGHGVVLEGPVCKQNQVVGWHTSGDANKLQRFHVAMSGFAFNSTMLWDPRLRSHKAWNSIRHPEMVEQGFQGTTFVEQLVEDESQMEGIPADCSQIMNWHVPFGSESPVYPKGWRSAANLDVIIPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1E1</accession>
    <name>A0A8I6X1E1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X1E1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0204630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="99" mass="11844" checksum="7F047F0D27CC94D0" modified="2022-05-25" version="1">MFKCHYTVRVWNLIYVWLGRQSVTPSWHDLHQVKQWWNLGKGQPRYSRKVINSLQMLICWEIWNERNARVFRNKASLSTRVVAKIREGAKTWVVAGFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1J8</accession>
    <name>A0A8I6X1J8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123424608</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>IDENTIFICATION</scope>
        <source>
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        </source>
    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="3">subsp. vulgare</strain>
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        </source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <location>
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        </location>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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    </evidence>
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</entry>
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        <lineage>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <location>
            <begin position="2"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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    </evidence>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">subsp. vulgare</strain>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:trichome morphogenesis"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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            <end position="159"/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="515" mass="56600" checksum="030FBF08B5679DEC" modified="2022-05-25" version="1">MDDGPRLAGEPAHVSSPASSSSSRASRPRKGVRLRPLRRRRGPAPATLASRGGDGDGEDNGGAAAQDDLALPLGMSFAAVLARVMNSSNDSGERLHPVILSKICTSAVKESLANTYGDRFDSFMRNFENSFSSTLRTLHRINEIPVNQRSPTPEYSSTHGGSGAVGNSSAVDLQNHTKEIEQDLVNSAESQLVLYARDNQQLADCAHTRSSRVADRCILSAFERSVKEQARSNELKECEISLSMRKLQLKQSELALSSSSHMLEKIKLSFGFQKASFQGEKFRTQMQDTRHAEILRTLIDFLVSAVLIMSVCFAYGTYVHSYQRITDVTAACSAASRGSKSWWVPNSVSNFNSGLQFIRCHVIAATRMCFGIVMIVAIAWLASQRSALTGSNMPITFNFILLGVICGFAGRFCANTLGGDGNIWLVFWEILCFIHLLGNCYPSVIYHVLHGPISIFHSKDGVWLPYWVRRCIFYAALGLVLPVLTGLLPFASLSNWKDHFSEEIKSLFVGDKVEA</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413710"/>
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    <dbReference type="KEGG" id="hvg:123449919"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413710.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="48104" checksum="01AD736E500B73CE" modified="2022-05-25" version="1">MAMSKQQQGDPAAFLDLVGPDLSACVFARLHDPADLARAATVSRPWRRFVEGNGFLKKACVRKFPELAVLAGAVEVRRSPAPSPGPSAEANEHRIYSHLYGALVPDARPAVDCILHCIGASSTDNFPDETIDNTLEPQDRVNNRFSYWSSAGQDDPDVPETLTYRLGSDICVVDEIRIQPFKAFFQIGHPIYSSKMVRFRMGHCKLPRRSESFITDDDDNQAVIADENYIWTYTSPEFLMFQENKLQTFKLPRPVLCIGGVVKIELLGRVQKQATDDRYYICVCHAQVVGRSLSPVFMVDINDSGGNAILKHLPEAKNLTVTEVVQDSATDSQEWKDALASYREMRHLVTLMNVLQEGPAHPAQDLHGDGDGDALLQEIQEMQGDANLLQQLQVDAQLLYLLQDGVLQLQDEDGLQAMEEDDDGGVSDNDPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6G9</accession>
    <name>A0A8I6X6G9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123445283</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978182.1">
        <property type="nucleotide sequence ID" value="XM_045122247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6G9"/>
    <dbReference type="SMR" id="A0A8I6X6G9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311640"/>
    </dbReference>
    <dbReference type="GeneID" id="123445283"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0260280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0260280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0260280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0311640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445283"/>
    <dbReference type="OrthoDB" id="646178at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1129">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="190"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="464"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="606"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="942" mass="104444" checksum="606204BFCB591F82" modified="2022-05-25" version="1">MAETAITTVLAKVAELVAWEAAVLLEVGDDVRLLRDKLEWLHTFIRDADRRRRLRDDEFVAVWVRQTRDVAFEAEDALDDFLHRAGRHRRRRSSPAPPLPGTGARCSGWRWSWRRWRPRCAGLQVALRHDLSARVRQIRKRLDEISANRAAYHIEHAASPAWAASSATTLAAWDDLEEYTVGFGKYSDMLKEQLLDVDTVPGRALVSIVGESSIGKTTLARKVYQSPEVRNHFAIRTWTVLPPNSRPADVLRDIHRQATSQLRRSPSNGQNAEDGGCDAKGGKDISNSLFRNMTGRRYLVVVDGSIAVADWNSLRTSLPDEGNGSRVVLVTDAAGLEVVGYAAGPTYDPIELPRLSAENTYELFRRRVFGHRGDCPGRYKSRYYQDVFRITRGLPLSIVILAGVLRSKELPAEWDQVMAQLLAAKDQPQHCKSGSGGARKIMSLAFDDLPHHLKSCFLYFAAMPESAPVDAARLVRLWVAEGFVRPRRGSTMEEVGQGYLKELISRCMVQLVDKDEFSTVTAVVVHDRLHAFAQEEAQEACFVESHDSTDVLAPATVRRLAVQNTTDRHVHLGNALPKLRTIVCDFANGGAAKPSVCIHSTDLGFLHASKFLRVIDIHGLELKKLPDELGSMIHIRYLGLQCGQLERLPSTISKLVNLQSLILKGRSGGVLGVTAAFWTIPTLRHVVAPFALPRCLGDLYSLQTLHGVQPRCWDTRAIAGNPLGRATNLRSLELSGLTAAHAGALMTALESLDLLVHLVLQGESLPPAVFTVASLRRLQSLRLVGAMDAPEEEDEDAGDEVAIRYIRPNLTRLSMWGTMVGQGFVDMLGELPSLAELTLMWGAYEGERLEFGDGAFRSLQKLRLGLPDLEEWAVSAGSMAALGRLTLLRCAKMEMLPEALGGMKELEEVVLYSMPKMVGRIKEDGGQDHHKIKHVPVIQTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6S4</accession>
    <name>A0A8I6X6S4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6S4"/>
    <dbReference type="SMR" id="A0A8I6X6S4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049140.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0061830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="102" mass="11406" checksum="060880D5C73A0DF6" modified="2022-05-25" version="1">MTLPNTLMPADQIFEFVRAADCYPNVSIAYRILLTVPVTVASAERSFSKLKLLKNYLGSTMSQERLNGLAICCIEKDGLDNIDLDTIINDFASKNARRSRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X841</accession>
    <name>A0A8I6X841_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3'-5' exonuclease</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Werner Syndrome-like exonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123431492</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044971245.1">
        <property type="nucleotide sequence ID" value="XM_045115310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X841"/>
    <dbReference type="SMR" id="A0A8I6X841"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096810"/>
    </dbReference>
    <dbReference type="GeneID" id="123431492"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0079870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0079870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0079870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0096810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431492"/>
    <dbReference type="OrthoDB" id="1920326at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000114">
        <property type="entry name" value="Werner Syndrome-like exonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF109">
        <property type="entry name" value="3'-5' EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="11">
        <location>
            <begin position="100"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="295" mass="32875" checksum="1D4C3E82A8104FDF" modified="2022-05-25" version="1">MESGAPHALPCPVDDDDDFYWDAEAEAELQAIEAAHAAESAKRRRLPDWSNTPAPAPVRPRPNPVRAAASASSPSSVLSPPTCQGSVKARYQQVAFRGKIVYCRTATEVDKATREIMRKIESMKASGPVSLGFDLEWKPFPRRGEPPCKVALMQLCMDKTHCYVMHIIHSGVPPILKSLLEDSSSVKVGVCIDNDARKMFNDYDVRVQPLMDLSTVANLKLAGPYKRWSLAALTEMITCKELPKPGNIRMGNWEAFVLSKKQLEYAATDAYISWYLYEVLRSLPDYTPDIETEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAE9</accession>
    <name>A0A8I6XAE9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123445133</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978008.1">
        <property type="nucleotide sequence ID" value="XM_045122073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAE9"/>
    <dbReference type="SMR" id="A0A8I6XAE9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307420.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307420.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307420"/>
    </dbReference>
    <dbReference type="GeneID" id="123445133"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0256850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0256850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0256850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307420.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307420.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445133"/>
    <dbReference type="OMA" id="MEMGVAK"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000014">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF241">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="272" mass="29490" checksum="16F47B43B762DB4D" modified="2022-05-25" version="1">MEHIARFFFGVSGNVIALFLFLSPVVTFWRIIKRKSTEDFSGVPYNMTLLNCLLSAWYGLPFVSPNNILVTTINGAGSVIEAIYVVIFLIFAERRSKIRMLGLLSVVTAIFTTVVLVSLLALHGKGRTVFCGLAATVFSICMYASPLSIMRLVIKTKSVEFMPFLLSLSVFLCGTSWFIYGLLGLDPFIYIPNGCGSFLGLMQLILYAIYRKNKGPAAGAVPAGKGEDADEVEDGKKAAAAVEMGEAKVNKANDDSAVDVDEQAVDKVASQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAV7</accession>
    <name>A0A8I6XAV7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAV7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0351160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0351160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0351160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0351160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0351160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0351160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0351160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28752" checksum="A7F28827C23170B0" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTTMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVWVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRMELYATRAHLWTALTQLAPVVQTGYGEMEMLNPVRTHLPANVDRPAIGGVTPLRGPLLPPVGGPRPHPCPYGSQGSQASVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDC0</accession>
    <name>A0A8I6XDC0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975796.1">
        <property type="nucleotide sequence ID" value="XM_045119861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDC0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255870.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255870.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255870"/>
    </dbReference>
    <dbReference type="GeneID" id="123443481"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0255870.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255870.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443481"/>
    <dbReference type="OrthoDB" id="77878at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265:SF0">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255870.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="445" mass="49584" checksum="46794175E38E69C0" modified="2022-05-25" version="1">MPRCPSLRFLLAPNSRRGGGGGALRLLETSGRDVLSEAVGMWGRHYWGGRRPSGGAAGAEAGGGVVVMFAWLSSKERHVRAYVDLYAALGWACLVCHSDFITLFMPEKAAVLADRVLTELVKELKIRSVPVVFASFSGGPKGCTYKVLQLIERRCKGQLSLDDYQLVRDCLCGQIYDSSPVDFVSDLGTRFLLHPSVLKMPEPPRVLSWMTRGIASGLDTLFIGKFEAQRKEYWETLYSSVHVGPILIFCSEDDELAPCSMVQNFGRRLLELGGDMNLVKWHSSPHVGHYKHHSEEYRAAVTKLLMKASALYTSRKRLNDYSVGTSTYSDTPYSSRNVHKTAESSSDRLGRVSADPADHFFLPSSMEYHESSNEVLKPELFNMPSVESTKNPDGVLGKMLYDVCVPKNVEGWDFKLASIDGQLMHFTARQHGTFNPTKCIRRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGI8</accession>
    <name>A0A8I6XGI8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Peroxidase</fullName>
            <ecNumber evidence="3 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123427679</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 19"/>
    <dbReference type="RefSeq" id="XP_044967712.1">
        <property type="nucleotide sequence ID" value="XM_045111777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGI8"/>
    <dbReference type="SMR" id="A0A8I6XGI8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0192410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0192410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0192410"/>
    </dbReference>
    <dbReference type="GeneID" id="123427679"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0159520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0159520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0159520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0192410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0192410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0192410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427679"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000007">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="6 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035340243" description="Peroxidase" evidence="19">
        <location>
            <begin position="30"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="30"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
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    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="75"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="124"/>
            <end position="326"/>
        </location>
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    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="201"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0192410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="330" mass="35457" checksum="83E070A9794EBA57" modified="2022-05-25" version="1">MALLSAMQGKAALLVVALAVLWLAAGSHAQLQNGFYKGKCGGSDVEVIVQGIVKARFGRDAPIVAHLLRLQFHECAVNGCDGGLLIDGPGTEKTAPPNLSVKGYDLIADVKAELERRCPGVVSCSDIEILATRDAVALAGGQNYAVRTGRRDRRQSKAADVILPAPESTVAQATAFFGKLGLSQMDMVLLLGAHTVGVTHCSVIKSSRLYSYGGRAGATDPSLDPYYAFVYKTYVCPNTASSDDNIVFLDDQPSALKVDTSYYKNLQKRRGVLSVDQNLYGDGSTRWMVDMLANSDNFSWLFPVALVKLSEVNVLTGTQGEIRRVCNTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XH57</accession>
    <name>A0A8I6XH57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable glucan 1,3-alpha-glucosidase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glucosidase II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123449301</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982397.1">
        <property type="nucleotide sequence ID" value="XM_045126462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XH57"/>
    <dbReference type="SMR" id="A0A8I6XH57"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396220"/>
    </dbReference>
    <dbReference type="GeneID" id="123449301"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449301"/>
    <dbReference type="OrthoDB" id="3237269at2759"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000046">
        <property type="entry name" value="Glucosidase alpha, neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035164679" description="Probable glucan 1,3-alpha-glucosidase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="14">
        <location>
            <begin position="85"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="13">
        <location>
            <begin position="350"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="15">
        <location>
            <begin position="686"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="915" mass="102942" checksum="2DF6D48CC5B36C17" modified="2022-05-25" version="1">MDPPPLPRVLRLALLLLLASPAALAWKKDEFRNCNQTPFCKRARTRAPHSLDAPLSLVAGSLAVSPEGSLTAELSHPSRPRPLLLRLSALPPHALRLQIDEDYSANTPPSRRFHVPDVLLPDVDSRALHLSKPKTVGSVSTVALSSDLDVVVKHDPFELTVRRAGSGDPVLSFNSHGLFDFEPLQESKPEGETWEEQFRSHTDTRPRGPQSITFDVSFYGADFVYGLPEHGSTELALRPTRGPGVEESEPYRLFNLDVFEYLHDSPFGLYGSIPFMIAHGASASSGFFWLNAAEMQIDVLAPGWDGAASSENGRIDTLWMAEAGVVDAFFFVGSKPKDVIKQYISVTGTPSMPQQFAVAYHQCRWNYRDEEDVGGVDAGFDEHDIPYDVLWLDIEHTDGKRYFTWDRSTFPNPEEMQRKIADKGRKMVTIVDPHMKRDSGYYLHEEATAKGYYVKDASGKDYDGWCWPGSSSYPDMLNPEIRDWWADKFSYQNYKGSTPTLYIWNDMNEPSVFNGPEVTMPRDAIHNGDVEHRELHNAYGYYFHMATSDGLLKRGEGKDRPFVLSRAFFAGSQRYGAVWTGDNTADWDHLKSSIPMVLTLGLTGMTFSGADIGGFFGNPEPDLLVRWYQVGAFYPFFRGHAHHDTKRREPWLFGERRTALMREAIHMRYSLLPYYYTLFQEASVTGVPVMRPLWLEFPDDKETYNNGEAFMVGASILAQGIYEEGQKSVSVYLPGTELWYDLRNGSPYKGSVSHKLQVSEDSIPSFQRSGTIVPRKDRFRRSSTQMVNDPYTLVIALNSSGYAEGELYVDDGKSYDYQQGAFIHRRFVFADNKLTSINIAPDSLTKKVYSTECVIERIIVLGLPSGAKKAIIEPGNQEVEIDLGPISLRSGSKSVAPTVRRPNVRVVDDWTIRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLM7</accession>
    <name>A0A8I6XLM7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0258980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF49">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="65614" checksum="DD523123023C84B4" modified="2022-05-25" version="1">MSPLRPAMLHHIDPARRPKDSRSVAVPSPPVDQAIFFLRSHAVTLSAADGVNASSPMAVGRALEAQLVVPEHSLRVTAHHPEHYLVIFTQPAQQVNALRRASIRVDGAVFNVASWHEHDHASFGSLLLHVRVIIEGVPMHYWSVEGAEEILGRRVRVDRLDSRTLERGHTKTFACWVWTDDVGNIPTKHCLGILPRGAGHVEEMVGFSPPDRRVAPPPATAEYSMLIHVDRVEDWTPPSPRSSHSERSGIPSSDSDGDAAPFPTIAPASWTMGIEDCQHGGRKKHQARAPVASVGCRGMARSGREQDGDGDGDPRGENTAPGRTFFSAVAALRPPPPHNHGLHGSAAALQRQDAGHGTPQADVAARGGLRPAQAREAHAETTGKDPVDEFFKTANKPPMTSPIVDGMAVDVQAAADAVLAETLHFDEGSLLEAAGEALQLDAFSPTLSDYDHFNRATPSSARMMEVQLGAVTSRVSQLELAEASGSKEPCLFRECRPPLLAVPPTRRSVPPPKNRVQDTPTRHNARQAANTSTVPVAQRASLRLVKELGLLGPKEKMTEDVAKALIPRFDEPLSDSDIAVIAKLTRLDGEALTAMARMAGPDVVAEKAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRH1</accession>
    <name>A0A8I6XRH1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123428252</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044968377.1">
        <property type="nucleotide sequence ID" value="XM_045112442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRH1"/>
    <dbReference type="SMR" id="A0A8I6XRH1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207910"/>
    </dbReference>
    <dbReference type="GeneID" id="123428252"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0172870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0172870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0172870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428252"/>
    <dbReference type="OrthoDB" id="644067at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF429">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="169"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="121"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="200" mass="21885" checksum="B4DB2896FA7C474C" modified="2022-05-25" version="1">MEELWKGMSLCSTPVALQPYHHLHSPAAAPYRGAGYHQDYLAGAVPQPPRTPPHHTALTLEFTCLGGTGAANSATSSGDDPAGCHFVFSASGGGSRRATAVQPAVAGDRRQRRMIKNRESAARSRARKQAYNNEMEAELAQLRRENRMLIQREQDLINDRLARAAQIAVPDCSSSSSSRRSTLQQKQQQQQQRCRSAPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRP1</accession>
    <name>A0A8I6XRP1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleolar protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123399758</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950076.1">
        <property type="nucleotide sequence ID" value="XM_045094141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRP1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493910"/>
    </dbReference>
    <dbReference type="GeneID" id="123399758"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0409330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0409330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0409330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0493910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399758"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="852"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="280"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="852"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="930" mass="105790" checksum="A3AE005F006501E9" modified="2022-05-25" version="1">MAKTKPMAAGAASADKKTNKKKGKGTMKGRNGPAAVAMKARGAAAAERSNPFEAIWSRRKFDVLGKKRKGEERRTSRSRSDAVHKRENTLLKEFEQSAKSSVFHDRRIGERDETLPEYDKAILRQQREHMAKLKRASKYNLPDEEDDEDPHTLLGNDDFNEEVPIGDDSDEEGNTLSKKRLSLQSSDLPSETDLPGETHGHKSKKEVMSEIILKSKFYKAQKAKEKEEDEHLVDKLDSDFALLAQTPALLSLTESARVNTHKNNSSTIHKDSSGLTGKEIFNKEKPDAYDKLVKEMVMDQRARPSDRTKTPEELAQEEKERLEKLEKERHKRMLGTAESSDEEDDDSEDDDHRMRADNSKPISGDDLGDSFSFDEPTKGKGLVDEIYEREGRKIGEGLASDDEGSDDSGEDEDEDDEEDAGDEEDSSDNDFRNMPARDWEQSDDDEVAVEEDEKDDVKDKEQAIGDKVMKTNAQNLKTVSNAKQKPHGKDEDLPFVIEAPNNLQDLCSLVDGRSETEIAEIISRIRTCNSIRLTPENRKKMQVFYGVLLQYFAVLATQSPVKFKVIETLVKPLIEMSGETPYFAAICARERLIHTRARLCEDIKVPGKSSLPSLKTLLLLRLWSLIFPCSDFRHAVATPMLLLMCEYLMRCPIQSGRDAAVGSFLCSMVLAATKESKKFCPEAISFLQTLLVTSLKGEVSNQINDQFMELKTLKPWLYISEQVHEVNRMNILDLMSMDPDSPFFSSDNFKAGVLLSVAECLRGFVIIHEGLCSFPEIFMPVSSLLQKIMEKSKLPALLREIFQEVVDLIKKRSDEHHASREPLQMRKQKPEPIKQLNPKFEENYVKGLNYDPDRQRAEEKKMKRRLKSEKRGAMSELRKDNYFLAAVKEKDRMRHEQERSEKYGKALAFLQEQESAFKCGQLGKGKKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTR1</accession>
    <name>A0A8I6XTR1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="6">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123401040</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950691.1">
        <property type="nucleotide sequence ID" value="XM_045094756.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950692.1">
        <property type="nucleotide sequence ID" value="XM_045094757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTR1"/>
    <dbReference type="SMR" id="A0A8I6XTR1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580070"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580070.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580070.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580070"/>
    </dbReference>
    <dbReference type="GeneID" id="123401040"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0580070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0580070.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580070.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401040"/>
    <dbReference type="OrthoDB" id="585369at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF18">
        <property type="entry name" value="VACUOLAR PROTON PUMP SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="8">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="7">
        <location>
            <begin position="147"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="9">
        <location>
            <begin position="383"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="488" mass="54229" checksum="C4E62E8881B67C0F" modified="2022-05-25" version="1">MDLAKDGAEMEEGTLEIGMEYRTVSGVAGPLVILDKVKGPKYQEIVNIRLGDGTTRRGQVLEVDGEKAVVQVFEGTSGIDNKYTTVQFTGEVLKTPVSLDMLGRIFNGSGKPIDNGPPILPEAYLDISGSSINPSERTYPEEMIQTGISTIDVMNSIARGQKIPLFSAAGLPHNEIAAQICRQAGLVKRLEQSKHAAEGGEEDNFAIVFAAMGVNMETAQFFKRDFEENGSMERVTLFLNLANDPTIERIITPRIALTTAEYLAYECGKHVLVILTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLATIYERAGRIEGRKGSITQIPILTMPNDDITHPTPDLTGYITEGQIYIDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRRDHSDVSNQLYANYAIGKDVQAMKAVVGEEALSSEDLLYLEFLDKFERKFVAQGAYDTRNIFQSLDLAWTLLRIFPRELLHRIPAKTLDQFYSRDATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTW6</accession>
    <name>A0A8I6XTW6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123452837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522060.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0522060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522060.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522060.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="54691" checksum="961F916CABB656C0" modified="2022-05-25" version="1">MASSEPPPPSKMKSAPAAPTTIRGLGDDLLREVFLRLPSLPSLVRAALTCPAFLRAVRSSPAFRRRFLERHPAPLLGIFLDIYKPAMPAFVPVRRRSDPDHAAAIHGADVFLARLPDDEDAGEDDGPGWSMTSCRDGYVVLVNRSTKRAAVYDPLTRGLHLLRAPPVEICEEDAGKTKFEYHVVAPDKDRRSFRVLCISRKMDEEWWGHQVQVAVFSPASREWEIFPKAGSLQNWAIGNGKLVDGSVYWTSGRGSGQFHVLNTGTLQFSEIDLPPERNVWGSALPPEPNVWGSPERNVWGSALPPEPNVWRLPEPNVWGSSEPNVWGSSEPNVWGSSEPNVWGSHGGWGSALPAFKVGETSGGKPCLAREAELTLEVWVWRAAAADDNVDRWMPHTTFPMPDAIGKLELQTADEGWRSPIQVVTIISGTVYLSFNQRSFGTKDTTWLLSFCIETEELKKVCPITCSIDESYYPYVMAWPPVLVGCKVNSELQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUT0</accession>
    <name>A0A8I6XUT0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUT0"/>
    <dbReference type="SMR" id="A0A8I6XUT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0412320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="27014" checksum="5343AC6A710B517B" modified="2022-05-25" version="1">MATFIPVKFTEFCQPDTTMPFGKELVQITKDLRIALLTITGKPTSSYPEDSRYRIEVHIPGRTFQPRTEPMDFKFIAPNWILGRDMAIHCALGRIKEEYKGCPISPDLFTVSRRNKDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLRELELEEAAREAHYEHELEAKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPVIMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XW97</accession>
    <name>A0A8I6XW97_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XW97"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0235980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0235980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0235980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0284540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284540"/>
    </dbReference>
    <dbReference type="OMA" id="SDIAPRW"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF38">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284540.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="239" mass="26415" checksum="5943B6943AA15B4B" modified="2022-05-25" version="1">MKSRNLYSLREDLNNAAVAVIWIPLFFWLPIGCMFFRLNGLPPKFSLQLSEATSPEDPQTALPADPISSTSTAFNITLHAVNRRPGDRCYRHGEAAVLYSGLTVAWGRTPRFCVGTMDARDVTVVAWADDGVALPALLGDQMAAERRAGSVELEVDVRLFRGDDGSARPTWMRCKVTTGGPKPSDGAPCTVFARQNWVSDISPYWMQTLIAYGCSQIETIVHGLFNQFVLIKKSINLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XX63</accession>
    <name>A0A8I6XX63_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XX63"/>
    <dbReference type="SMR" id="A0A8I6XX63"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0545560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545560.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12172" checksum="96B309F4983B53FF" modified="2022-05-25" version="1">MNAVANQPVAVSISSHSSHFHHYKGGIYNGPCSTTKLNHAVTVVGYGQQQQNGADSVHASAPGAKYWIVKNSWGTTWGDKGYILMKRGTKHSSGQCGIATRPVFPLMKGGRSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYL3</accession>
    <name>A0A8I6XYL3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYL3"/>
    <dbReference type="SMR" id="A0A8I6XYL3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0624940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="54"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25179" checksum="7ECC3156CDB3FB17" modified="2022-05-25" version="1">MPTRFHKVLAHGTTMLDVVYTNESREVPFFLEQLKEKWLDTAMDHEKFLGPDLEYTVDQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSGITFATVDIRNDKLKMRYNFGIEIPTGCLIDLQTVFRLRLVRTSMAHMAVALIDEEYGDMKTSFPKSQHKLWEKAPLDPINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZL7</accession>
    <name>A0A8I6XZL7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC123404517</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044954383.1">
        <property type="nucleotide sequence ID" value="XM_045098448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZL7"/>
    <dbReference type="SMR" id="A0A8I6XZL7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561260"/>
    </dbReference>
    <dbReference type="GeneID" id="123404517"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0466170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0466170.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0466170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0561260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404517"/>
    <dbReference type="OrthoDB" id="647974at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000403">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000469">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000530">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF18">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035263412" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="34"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="771"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="800"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1056" mass="116542" checksum="3C7A63ADA047AB45" modified="2022-05-25" version="1">MHTLHFSNKTCSKIPSLGLALVLLLCWASPASCCTDQEKGALLQFLAQLSQDHGLSATWRNTTDCCNWEGITCRQDRTVTHVLLPSKGLEGHISQSLGNLTWLQHLDLSDNSLSGGLPLGLVSSRSITTLDVSFNQLNGTLQEMLASSSTPGRPLQVLNISSNLFVGQFPSTTWKAMENLRALNASNNSFTGQIPTQLCSTFPSLEMLDLCFNKFSGRVPPGLGDCSKLRELRAGYNNLSGRLPNELFNATSLEYLSFANNGLYGVLDNTRIVNLRDLVTLDLGGNQFSGKIPDYIGQLKRLEEFHLNNNNMSGELPYALSNCTNLITIDLKSNKLRGELSNVNFSNLPNLRTLDLWSNNFTGTVPESMYSCSNLTALRLANNKLYGQLSSRIGNLKYLSFLSLGKNNFTNIANALQILQSSKNLTTLLISFNFRAELMPEDDRIGGFENLQVLDMDGCQLSGKIPLWISRVTQLKMLILRSNQLSGSIPDWINSLSRLFYIDVSNNTLTGEIPLNFTEMPMLKSTDNTTHFDPRVFELPVYTGPSLQYRVVTSFPTMLNLSNNKFSGVISPQIGRLNLLAVLDFSFNRLSGQIPQSICNLTNLQVLDLSSNNLTGAIPAALNTLNFLSKFNISSNDLEGPIPSGGQFNTFQNSSFNGNPKLCGSMLTHKCGKDSISPSSRKKRDKKAVFAIAFGVFFGGIAILLLLARLLVSIRQKGFTGKNRRESNGDAEESSFSSSSEQTLVVVRIPQGKGVENKLKFADILKATNNFDKANIIGCGGHGLVYKAELSDGSRLAIKKLNGEMCLMEREFSAEVDALSRAQHENLVPLWGYCVQGNSRFLVYSYMENGSLDDWLHNRDDGASSLLDWPTRLKIAQGASLGLSYIHDACNPQIVHRDIKSGNILLDKEFRAYVADFGLARLILPNNTHVTTEVVGTMGYIPPEYGQAWVATLRGDIYSFGVVLLELLTGRRPVSVFCTPKELVPWVLQMRSEGKQIEVMDPTLKGTGYEEQMLKVLEAACKCVDHNQFRRPTIMEVVSCLSSIKAEPEMQRSANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0R9</accession>
    <name>A0A8I6Y0R9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0R9"/>
    <dbReference type="SMR" id="A0A8I6Y0R9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0391980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0391980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0326100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0326100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0326100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0391980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0391980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0391980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF13">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
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        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="15977" checksum="2460308B9A1EFFBF" modified="2022-05-25" version="1">MIRCTDCPRIAPLKRLTSKEEKNGNFGREFVKCESKPEGQIVKKCHHFEWMDDYIQRLQGLALLDSRGDAIREFNLPHDSAALAAAARPEYPTVVDVELKTQLKKMNKNFKQLIELKKQSNLIALGILALGTFYLMAISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y107</accession>
    <name>A0A8I6Y107_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y107"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0310170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482">
        <property type="entry name" value="OS11G0632001 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482:SF24">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="26960" checksum="AABCAD9E7476747E" modified="2022-05-25" version="1">MLEVMSTPQQPTAMMRFDTLEDAEKHYKMYARQKGFGVRYCFRKKSEASGELIRASLVCHRAGLKIKRKVDTQNPQPIAPERRRNTSERTNCPARMFVKRRDNAWVVTEINDDHNHPLIKKWSLTGYLRSDRHIPEEEQQFVKLLHSCNLEPSRQMQLLTELHGQREAIGYTDKDLANLLAKFRAEHKYTDMQDTSEYFKSSQHLDKDFFTSTSLMMKIRSNAFTRLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y141</accession>
    <name>A0A8I6Y141_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123411873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960766.1">
        <property type="nucleotide sequence ID" value="XM_045104831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y141"/>
    <dbReference type="SMR" id="A0A8I6Y141"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0642610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0642610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0642610"/>
    </dbReference>
    <dbReference type="GeneID" id="123411873"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0533920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0533920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0533920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0642610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0642610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0642610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411873"/>
    <dbReference type="OMA" id="IAAWHDV"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0642610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="446" mass="47224" checksum="5B1AFD653878020F" modified="2022-05-25" version="1">MPTVNIVEVDHVVASVPPPEDLVVRLSALDAPWVANPLIQRVLLFDDAGGQQHPPFDSLVGSLRASLASTLARLPPLAGRIVFLPSTGDAAIDCTGREGGGVRFAVAESDDADARRLAGDADHDVAAFEALVPKLEVGALPAEVLAVQVTRLKGGVAVGVALHHAVADGRSVWMFLQAWAAACRGDDAAVAAVAFDRAVVAIPGGEELARSTLRKYAPNLPLAANLFPSAPIKLPRRTFTITAKHIHHLKHWISGQMTSGKAATTPMSSSFVAIAALAWASFVRSKHPAAISSDHDVYLFFFIDCRGRAGIDPPVSENYFGTCITGCLVKAMARDLLAADGVAAAAAAIQREVRRASEDPLGLWDWMDIVSWVPLDRLVGMNGSTRFKAYEVADFGWGAPSRTELVTMTDGRVVLVAGKSGGVQASVCMHPDHARSFNSHFLDSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y253</accession>
    <name>A0A8I6Y253_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123452300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044984877.1">
        <property type="nucleotide sequence ID" value="XM_045128942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y253"/>
    <dbReference type="SMR" id="A0A8I6Y253"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0511630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0511630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0511630"/>
    </dbReference>
    <dbReference type="GeneID" id="123452300"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0424970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0424970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0424970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0511630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0511630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0511630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452300"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000061">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000108">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1412">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 83A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0511630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="455" mass="50168" checksum="BB5242EFACB5BD7E" modified="2022-05-25" version="1">MAKGHVLVLPMPCQGHVVPLMELSHRLVDHGFEVTFVNTDVDHALVLAAMAPDAGAALRGIHLASIPDGLAGDEDRKDLNKLIDAYSRHMPGYLETLVVDMEAAGRPRVNWLVGDVNMGWSFEVAKRLGIRVASFWPASAACLAIMLKIPQLIQDGVLNDKGWPEREETLQLAPGMPPLHTSLMSWNNAGAPEGQHIIFQLVCRNNKLNDLAEMVVCNSFQEAEAGAFKLFPNILPIGPLFADREFQKPVGNFLPEDARCLKWLDARPDGSVVYVAFGSMAIFDPRQFQELAEGLELTGRPFLWVVRPDFTPGLSKAWLAEFSQRVAGTGMIVSWCSQQQVLAHRAVACFVSHCGWNSTMEAARNGVPVLCWPYFCEQFLDRSYVTDVWRTGLAVSPGEDGIVSKEEVRSKVEGVIGDAGIRKRASWLKDTASRCIGEGGSSQRNFKKFIDLLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2X5</accession>
    <name>A0A8I6Y2X5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2X5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0411360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0411360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0411360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0411360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0411360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0411360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001380">
        <property type="entry name" value="RNA-binding (RRM/RBD/RNP motifs) family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="29"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="500"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0411360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="619" mass="66315" checksum="773CCA92FDC6B796" modified="2022-05-25" version="1">MPMVHVAQKKPVEEGIKETSDVVADSPHKIFIAGISGVLSSEMLVEIVSSFGKLAAYHFVCSEDLGGRCAFLEYIDHSVTNKACAGLNGMKLGGCILTAVQVFPNPLEVRNEASAFYGIPDSAKALLEEPTNVLQLKNVFDREEFLALSKPELEEILEDVRMECARFGAVKSINIVEYPASSDSITEDVLVEPKDEPVKLEPPECRTNDNCAETGTDCSLPSRSIVVPSNPREITGVDGISAFQDHKEPDTLREFDLPAAADQYTDLDDSHARAAGLTMDRQMESDHMDAMQVDQDATAVGDIHARAACSTVDQQTEADHIDSIQADQDAIAVVDIHGRAADPTVDQQTEADHMDSTQADQDATAVGDIQARAADPTVDQQTEADHMDSVQADQDATAVGDIQARAADPTLDQQVEADHMDSMQADQDATAVGNVQARAADPILDQQTEADHMDSTQTDQDATAVGNVQARAADPILDQQTKADHMDSTQTDQDAAAVGEDSLGEGHAGPTTLETCGSTTPGDVAGRSETENEQQSTDDVSESGAEKLPVADTRDDAPTRDDSLVSDTIVPEAGSILVEFMRKEAACLAAHSLHGRSFGDRTVSAGYAPHDLYLQRYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4Q6</accession>
    <name>A0A8I6Y4Q6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lariat debranching enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447631</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lariat debranching enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980172.1">
        <property type="nucleotide sequence ID" value="XM_045124237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4Q6"/>
    <dbReference type="SMR" id="A0A8I6Y4Q6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338760.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338760.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338760"/>
    </dbReference>
    <dbReference type="GeneID" id="123447631"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0282600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0282600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0282600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0338760.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338760.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447631"/>
    <dbReference type="OrthoDB" id="407609at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008419">
        <property type="term" value="F:RNA lariat debranching enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00844">
        <property type="entry name" value="MPP_Dbr1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000035">
        <property type="entry name" value="Lariat debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007708">
        <property type="entry name" value="DBR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041816">
        <property type="entry name" value="Dbr1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849:SF0">
        <property type="entry name" value="LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849">
        <property type="entry name" value="RNA LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05011">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01124">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lariat debranching enzyme C-terminal" evidence="14">
        <location>
            <begin position="235"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338760.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="407" mass="46470" checksum="F51D5D40AD330631" modified="2022-05-25" version="1">MKVAVEGCMHGELDKVYDTMRRLEAAEGIKIDLLICCGDFQAVRNESDLQCVNVPPKFRTMNSFWKYYSGQAVAPYPTIFIGGNHEAANYLWELYYGGWAAPNIYFLGFAGVVKFGNIRIGGMSGIHKQNDYYRGHHERPPYNEGTIRSVYHVRHYDVLKLMHVKEPLDIFMSHDWPLGITEYGNRERLLREKPFFKEEVDKRTLGSESAAKLLNKLKPPYWFSAHLHCRFPAIIQHGEDGPMTKFLALDKCLPGRNFLQVIDIPSNPGPYEIQYDEEWLAITRRFNSAFPLTRMPCTIRNEELDIEDDRQWVRSKLNARGAKTFDFVQTAPPYDPSRPVYNPPVAVHCRNPQTESFLQLLELPYLLDSSNPGGVDTNVSSSQAAPALDDDAIELPDEVEDDEGDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4T2</accession>
    <name>A0A8I6Y4T2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4T2"/>
    <dbReference type="SMR" id="A0A8I6Y4T2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0424080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0424080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0424080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0424080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0424080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0424080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0424080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="347" mass="39751" checksum="E263E4996A10698B" modified="2022-05-25" version="1">MKAYLIELLRDHDVPKYRTQNAWSKEAWTSIVGKFNQRFDLSFTVIQVKQKEQDLKRDFKAIKDLISESGFGWDHDRKMVVAPDNVWAALEARKNKDALTWRGRSFPYYEDLFALYDGRYAQGRSCHGMDYYANKATQPSQFPASHSPQLQGLEAHLHTPTPTIHAPGDSSMQFHIEEDSENTNWLSSNNTFSQVEVNSTQGNDLTLHTPQVEAIPISSQHVGQTLHENPQVAHRNPRPSSSGPEANSTRRAKKQKTTSIDDFHERYLKLRREEIDRYAAIEERKLKDPFSIKKCIKALERLEGLSMADMLKAADIFAANKENREVFLSFSSNALRLGWLIGKVQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4T4</accession>
    <name>A0A8I6Y4T4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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        </source>
    </evidence>
    <sequence length="2421" mass="268206" checksum="80B889EC9D482309" modified="2022-05-25" version="1">MVPLNHPGVAAEVRLVLQGATDADRDSVSRELCQLVDYGHDGCLLMLQVCLDEVLLNDREAKNLQLKHDLLSSTFRYCLDKTYFSTCFCEALMQIKTATDGLLETLSSVLELSAAEKVGIGLALSDSDNSGMKLKGQQFAIAQIEELCLNPNQSIPNDQIHEIVVFLHQTDGLTKHMDTFSNIASLLEVGQSPFFAPIPKEQYDAQSINPSRHLEMYLDNTNDDFESLLSEIGKEISMADIVIELGYGCTVDSTQCKEILSTFQPLDDVAISKLLGAVIGTQNSLGEAHNTYATFVSAIRNTHLSESPQLTTWNTDVLVDSINELVPSTNWVHVMENLDHEGFSIPDEAAFCLLMNIYGRACKDPFPLHAVCGSMWTNTEGQISFLKHAISAPATIFTFAHSSRLLALPDFASLTPGNHAWSCLDLLEVLCQLAEVGHTVSVRSMLEFPLGHCPELLLVGLGHINTAYNLLQFEVLSCAFPALLKDATKRNVLNHLWHINPCLTLRGFVDAHSDPSCLLRIVGVCHDLKILSAVLESTPFAFSIRLAAAACRKDHSHLEKWLTEKLTVYKDGFVEECVNFLKEAMNATSYEVEGTMEQPQASVVDMYWEACPPVIKVLQSHLGQLLSNQLSDELRELYALYDSRNQGLVRDNPTSEGGSDDVEVEANAYFHQMFSGQINIDVMIQMLARFKESPDKREQSIFNCMISNLFEEYKFFTKYPDKQLKLAAVLFGSLIKHQLVAHLGLGIALRAVLDALRKSVDSKMFMFGTTALEQFMDRVIEWPQYCNHILQISHLRATHAEMVSAIERALARISSSQNEPNSGNFLSTEQLVSGSSSIETMEASEPSWQLMGTSPTQLGRTPYPLQQRQPSVLGDRSKVSIGTSQTKSILPSQPSVPPASADSAVNLKTTVPPSSLAHSTSMSNTAHSTNFLRPRITSTVLPRQHSYTTGFGNALNIETLVAAAEQRDSPIETPPSEVQDKILFMINNISTSNMEAKAKEFSEVLQEQYYPWFAQYMVMKRASIEPNFHDLYLKFFDKLNSRSLSKEMLKATYENCKVLLRSDLIKSSSEERSLLKNLGSWLGKFTIGRNQTLRAKEIDPKSLIVEAYERGLMIAVIPFTSKILEPCHSSIAYRPPNPWTMGILSLLAEIYNLPNLKMNLKFDIEVLFKNLGVDMKDVKPTSLLKDRGREVEGNPDFSNKDVASSQTQVSAEVSSGINPTVKHVDLQPEVNSTSRTLSLPPMLNQYTAPVRLPPNSMSEDDKIALMMPEQVPSHTLTQVPPSQTPLVSLSASPSPLSLTQLLSLIPHDEIRFKISSKLLSLGSQLQFSKIMGVALDKAIKEIILPVIERSVSIASKTTKEIILKDYSTESDYSAANRSARLMVGTLAGSLAHVTCKEPLRVALKSHLRSLIQNLTSNSETGDQVIHILINDNLDLGCASIESVATRKAVDSIEGEITQSFSQQRKKREAAGPAYYDSFTYAQGPFAPVPEVLHTKAESPAQQRVYEEFVHVWQSHGQSVGAAGSGSSGTAAVSSNFGVPRAYSPSLAPASSAFLSNQTAPLALTQPTELVYEELIPGAAPLSSDSPAQVGTSDSSGWLGGTIGDASTSPPLMSNDLPVGGITDLNAMMPPPATVSDNLGSALPDPLSTSDAMERYEQVSQKLEVLIAKDGKDVEIQSVIAEVPDILLKCVNRDEAALAVAQKVFKSLYDNSSNSGYVMCSLATLVAIRDVCNLVVKELMNWVIYLDDEKKFHSEIIVTLIRSDLLDIREYTIHLAKLIDGGRNKVATEFATSLVQTLITQDSASISELYDVLDALSKISRRPGSPDFLQQLTEIARNNANNAPGFAFGKDDRLRQSKDKKVLPSRANKEENSVNDPIVADSVAFQDQVAHLFSEWCQVCDHPSACDAAYSRYVLQLQHIGLLKGDEFTERFFRILTELAVAHSLVSEQITAPGGLSPQSSHISYFPIDSYSKLVSAVLKNCSVEIGPNKGSLLPKILLLTIRIIQKEAEEKKASFNPRPYFRLFINWLNDLITSEPHHDGTNLQVLTAFANAFHVLQPLRIPSLSFAWLELVSHRTFMARLLKGNSQKGWPFFHRLLIDLFKFMEPYLRTADLLEPVHLMYKGTMRVLLVLLHDFPEFLCDYHFSFCDVIPSSCIQMRNVILSAFPRNMRLPDPSTPNLKIDLLAEISVPPRIMSDVDGALKSKHMKTDVDEYLKRPEGSSFLSDLKQKLLLPQNEANVAGTRYNVPLMNSLVLYIGIQAVQQLQVNKANASAASGQQINHSTMDIFQIETATEFFKYLVTNLDTEGRYLFLNAIANQLRYPNSHTHYFSFIILYLFAEATQEHIQEQVTRVLLERLIVNRPHPWGLLITFIELIKNPRYNFWNRSFTHCAPEIKQLFESVAKSCGGAAGKAVDDGSDGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9J3</accession>
    <name>A0A8I6Y9J3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9J3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0485120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053151">
        <property type="entry name" value="RNase_H-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723">
        <property type="entry name" value="OS05G0353850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723:SF24">
        <property type="entry name" value="RNASE H TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="137" mass="15152" checksum="C585127DB0F1C9E8" modified="2022-05-25" version="1">MILRNASGGVIFAVYRKLFHCNDALEAELHAILEGIKLTVEHSNSTIMVQSDCVLALKAISDASLDRSTYDHMIREIKFLLSDRVFVSVQNSRDQNRVADCLANFGRCGDSTSCWLGQAPPCVSDLVAEDYNSVNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YA44</accession>
    <name>A0A8I6YA44_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flavonoid 3',5'-hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123412694</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961570.1">
        <property type="nucleotide sequence ID" value="XM_045105635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YA44"/>
    <dbReference type="SMR" id="A0A8I6YA44"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640130"/>
    </dbReference>
    <dbReference type="GeneID" id="123412694"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0531890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0531890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0531890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0640130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412694"/>
    <dbReference type="OMA" id="ACKEAMR"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000126">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF18">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="56615" checksum="8C49167D50C182E1" modified="2022-05-25" version="1">MQLAALCTDPFVLSCTFLCLLLHLALRRSLNRRSSSRLPPGPPGLPILGALPFVGPAPHAGLAALARKHGPVMYLRMGTCGVVVASSPSAARTFLKALDARFANRPAVASAADITYGRQNMVFADYGPKWKLMRKLASVHLLGPRAVADWARVRRDEAGRVLRGMKEAAEVTGRAVVVPEVLVCALANIVGQITVSRRVFDAQGDESSSYKEMIVSLLTGTGLFNISDFVPALSWMDLQGVQARLRRVHRQFDGLITKLLAEHAATAEERARDGRLDFVDRLRASSNDGGDGNDDGETITEVNIKGLIFDMFTAGTDTSSIIVEWAMAEMINNPSIMARAQEEMDRVVGRGRRLEESDIANLPYLQAICKEAMRLHPSTPLSLPHFSFEECEVDGHHVPANTRLLINIWAIGRDPAAWEDPLEFRPERFMSGPAAKIDPMGNNFELIPFGAGRRICAGKLAGMVFVQYFLGTLVHAFEWRLPDGEEKVDMAETFGLALPKAVPLKALVTPRLVPAAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YB17</accession>
    <name>A0A8I6YB17_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123449910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044983210.1">
        <property type="nucleotide sequence ID" value="XM_045127275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YB17"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413590"/>
    </dbReference>
    <dbReference type="GeneID" id="123449910"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33623:SF4">
        <property type="entry name" value="DUF4378 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33623">
        <property type="entry name" value="OS04G0572500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="49078" checksum="25B6CACD0A410C09" modified="2022-05-25" version="1">MGATAPLTLRDFLEMACDSSCSDGFRSYPRRLLPCPVRSEDEATQAKLRAEAAPVRLLIEADLRRSPSRTLSSFLFPRSPRSLAAISRFSRTLSRRLVFWRRHPDDDDDDGDDSERDSLGLPSPVVSSCSASDYAESEAETPGSPPVDAALEKPASKPSPSPSPSSSSGSVDPSDAGAKHTERLTDGVGSAEEGKQQLSPVSVMDFPFHHHHDDDNDDETSDAGTCSPATSFQHCLPDDSQRSNEAQAQELLLRKIRRLDGLAHAVCPVDLEAHFGGAPDRQPGESDDARTQTSNSGADHGAATTWSPRCRGADGTAERGPDEAHRLLARLLKDDGGDTAATTSSVATERLLLDFFAEGLGRRRASDAGLAVGAARPLDDVSVLVRAAREWVKGAGTRWGAEDVLFAGEAALADMERGRRWVCVAEEEREVGGLLEGLVMDAMVDELVRDLVLAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YEU3</accession>
    <name>A0A8I6YEU3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123412511</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044961401.1">
        <property type="nucleotide sequence ID" value="XM_045105466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YEU3"/>
    <dbReference type="SMR" id="A0A8I6YEU3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707240"/>
    </dbReference>
    <dbReference type="GeneID" id="123412511"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0586540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0586540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0586540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0707240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412511"/>
    <dbReference type="OMA" id="AIMAYCF"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF113">
        <property type="entry name" value="S-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="132"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="337"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="406" mass="46070" checksum="B54760773FA3F01B" modified="2022-05-25" version="1">MAKQLRVYQVWRGNNIIWCGGRLIFGPDAKATLLSFSLITAPVVVFCIFVGKNLIHIFPAYNAGYAILVVTVVLTIHVLLLLFLTSSQDPGIVPRNSNPPAEEFSHDSSAPHTLQFPRIKEIMVNGVPVRVKYCETCMLYRPPRCSHCSKCDNCVERFDHHCPWVGQCIGQRNYRYFFWFVCSAAVLCFYVFTMSALYISLLMKDHRSVVEAIKASPASVAVMAYCFICFWFVGGLTGFHSYLIATNKTTYENLKYKYSNQPNAFDLGCIHNCFEVLCTKRKPSRINLRAIVQEEHLASLPRISRSSVPEDETPHRPRAKVEDDLEMGLDILKTARRRSDELSDEELGSASNGARYRRSDSDTDIPVITRTITESSDQTRDLDFFSVTNAAHLSSPEQKQRPNELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF30</accession>
    <name>A0A8I6YF30_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123403677</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF30"/>
    <dbReference type="SMR" id="A0A8I6YF30"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000355">
        <property type="entry name" value="Wall-associated receptor kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF371">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="364"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="493"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="821" mass="90051" checksum="D4FE545E8693A58A" modified="2022-05-25" version="1">MTSQVNPACFVAWLFRPVAMGSSQIASTVGLSPSYTELTSPRTYIIGARAEQPTSSEEIMSRMATTTVLLLLIVLPLLGAASASAQRMALPECRDRCGNITVPYPFGIGAGCYRDDGLRGFQLECDDSGPAPPRLTVFGRYNRVAALSLASGEARAYLNATRECYNSTGGFVDRNNNTSYMSLLGSAYLFSPTENRLVALGCPNLGYFVDSAGYYVSGCMSVCRPSRYAMPGPCTGVGCCQSAIPPGVGFFEPHQRNFPPQQDDNSAFISNVTSCHYVFLVELDWFSYSDRVFLNRTDDFDVPVVLDWAVRNVGNCSAARRNATDLACRGELSECFDVANGPGYRCNCSRGYDGNPYLDGGCTDIDECQLKEEYPCYGICRNTQGGHTCQCPPGTTGDATRKDGCRQKDKFTLALKLVTGVSVGVFLLVFMCFWLYLGLQKRKLIKAKQSFFEHNGGVILQQQMRSYSGAAGGGGGGFKIFSEEELKKATDNFAADQILGRGGHGIVYRGVLEDKTIVAIKKSKVMEATETKEFAREMLILSQINHRNVVKLHGCCLEVEVPMLVYEYVSNGTLYHYIHGGEGLDTNNNKALDARLRIAAESAEALSYMHSSASPPILHGDVKTANILLDGSLTAKVSDFGASKLAPSDEAEIATLVQGTCGYLDPEYLMTCQLTDKSDVYSFGVVLLELLTGKKVLCFDGPEEDRSLVSRFTTAMKAGQHSELLDDQVRMEMGPEALEEATHLVMRCVSMIREERPSMKEVAEKLEALRRYQRNPWGQAGADPEEGQSLLHREQQRDVNYNFRPQDVLDLEEGSTYTFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGI4</accession>
    <name>A0A8I6YGI4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGI4"/>
    <dbReference type="SMR" id="A0A8I6YGI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0595670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0595670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0595670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0718200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000111">
        <property type="entry name" value="p-loop nucleoside triphosphate hydrolase superfamily protein with CH (Calponin Homology) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031852">
        <property type="entry name" value="Vik1/Cik1_MT-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF49">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16796">
        <property type="entry name" value="Microtub_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="12"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="631"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="331"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="431"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="715"/>
            <end position="722"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="794" mass="89799" checksum="0595D8CA045EB7C7" modified="2022-05-25" version="1">MSAAATAAESPERRRSEAVAWLRALLRESALPLPPPRASDDELRAALADGALLRAALDKLARPASPRGDEGGAAAAERDVGRFLAAVERMGLRPGFTPSDLQTGPMSAVVACLLALRDQFVSHAGEGWTCGLPQNGRMHTKGFPGKENGQVTQNSEARQDDKQMETKLQKVSKSPAVSEPSSVISRHELSSTSRHAGRNFHEVFQLRQGGYSDLPSSKISDMMKSTSLDNAPTQSLLSVVNGILDEIIERKNGEIPYHLAFLLRKIVLEVERRISTQAEHIRNQNNLMKAREEKYKSRIRVLEALASGTSGQMHVSSNATNGKAHVAAGPVHQMKMEKDKSEDKRQLLEEDLTKLTKDKENVTRLTKDKEDMARLLNDKEDIIRLMKEKEEMARLIKEKEDTVRLKKGKDGDRNQSADEHIAKPIMYKDELISLMKEKENYKVTIMKLKLELEAMKSSCEKSHILLETKNEDVLNLLKEKENSANVISQLRQELAIARISHETYIQDLKTTALQENRDFEQRIKDVELKLEDSTKRGRYLEELLESRIQTWQQKEIMLNQFVGIQMQNIQDLRLSSVSIRHEIQNCQKRWSEELSGLGQSLKVLTNAAEKYHSTLEENRKLFNEVQELKGNIRVFCRIRPFLPNEDHKSSTTEFIGDNGELVLADPTKNGKEGSKLFKFNKVLGPTISQDEVFKDIQPLIRSVLDGYNVCIFAYGQTGSGKTYTMTGPEDATEQELGVNFRALNDLFFISCNRRDTFKYEISVQMIEIYNEQIHDLLGSDGSEKKYPFVYDVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHY3</accession>
    <name>A0A8I6YHY3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHY3"/>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="20778" checksum="347CD9A02249BF0B" modified="2022-05-25" version="1">MWHYTGPEDSTRTNVVGVPEERVTSWVLQITGPCENPKGARRVKPYSADHPPPNQAWTNWFSPVSNGNPEEEEEEGSQEGSVESAEYVSDSGESEEEDSEEEEQDEEQDSPPPRPEHRTKRQHEPAVPSAPPASSSAPPTAPVVPSVRGTKRARDAAAEPAGQSSRAPKPSGPKPRKALPRMRVAILVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIF2</accession>
    <name>A0A8I6YIF2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731610"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.7HG0607070"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="173" mass="19438" checksum="D424C135A08D7DCC" modified="2022-05-25" version="1">MSFDSSVLLWHIAIDICFHHQSTTPRGQECAAQSRVISNYMTYLLSIRSEMLIVGSKVGIFIISCEDIELTLGHNSALHDERGLSQGILSKAQQPPLLDNTSIAGTSCRLAKMLMELQDEVERWEMVQGVWVEMLCYSVRRCRGYLHAKNMSELTELLLEYVMGLGPLVLGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YL87</accession>
    <name>A0A8I6YL87_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949399.1">
        <property type="nucleotide sequence ID" value="XM_045093464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YL87"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522160"/>
    </dbReference>
    <dbReference type="GeneID" id="123399034"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0434020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0434020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0434020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0522160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0522160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399034"/>
    <dbReference type="OMA" id="VNDESHF"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34574:SF5">
        <property type="entry name" value="CALCIUM-BINDING EF-HAND FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34574">
        <property type="entry name" value="CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="16775" checksum="42846FA07A7ADA2B" modified="2022-05-25" version="1">MSMVVLDGSTVRSFVADEAAFARSVDARFAALDANGDGVLSRAELRRALESFRLLDGAGFGSAEPAPLPGEVAALYDAVFEQFDADHSGAVDRAEFRGEMRRIMLAVADGLGCQPLQVAVDDEGGSFLLEAAEHEAAMIAARVQEDRDRAAAQEAADGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLE2</accession>
    <name>A0A8I6YLE2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger LSD1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLE2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0608720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0608720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0608720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0504710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0504710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0504710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0608720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0608720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0608720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040319">
        <property type="entry name" value="LSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005735">
        <property type="entry name" value="Znf_LSD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747">
        <property type="entry name" value="PROTEIN LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747:SF3">
        <property type="entry name" value="PROTEIN LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06943">
        <property type="entry name" value="zf-LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0608720.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8579" checksum="E1A1F45BD94A62B5" modified="2022-05-25" version="1">MAQFICGGCRTLLMYIRSAYTVRCSCCSTVNLVRPGNANSASYYGISSYELSIFMLLIHENFNLSEECGILLIYCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLT6</accession>
    <name>A0A8I6YLT6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123399221</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044949583.1">
        <property type="nucleotide sequence ID" value="XM_045093648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLT6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525940"/>
    </dbReference>
    <dbReference type="GeneID" id="123399221"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0437310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437310.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399221"/>
    <dbReference type="OMA" id="TERNPRE"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374:SF379">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="17017" checksum="844B39CEDCB7FF05" modified="2022-05-25" version="1">MKRLRFGEEPEVDGAAIAHEVMMLLAARGAAMGVGEPVTVARPRAFECKTCNRQFPSFQALGGHRASHKRPRGEPISAQAPAPVPARRHGCTVCGVDFALGQALGGHMRRHRAAGAEEDDSVRRPAIIELEPGEQRGLLLGLDLNATPAEQGLDLSLWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6YLW5_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC123412144</name>
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    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044961029.1">
        <property type="nucleotide sequence ID" value="XM_045105094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLW5"/>
    <dbReference type="SMR" id="A0A8I6YLW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0680310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0680310"/>
    </dbReference>
    <dbReference type="GeneID" id="123412144"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0564740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0564740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0564740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0680310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0680310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0680310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412144"/>
    <dbReference type="OrthoDB" id="1424968at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000006">
        <property type="entry name" value="NAC domain-containing protein 100-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF86">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="34"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="332" mass="36727" checksum="D5A0737B4D928523" modified="2022-05-25" version="1">MHQHQPAAAMGDALWELIGEEMAAAEAAAGEHGLPPGFRFHPTDEELVTFYLAAKVFNGTCCGGVDIAEVDLNRCEPWDLPEAARMGEREWYFFSLRDRKYPTGLRTNRATGAGYWKATGKDREVLNAATGSLLGMKKTLVFYRGRAPRGEKTKWVLHEYRLDGDFGAVRRSCKEEWVVCRIFHKAVDQYSKMMEMRNPYCYFPMTPHHPSFFQDAPPVPFPNPSQLIPFHHDLPHLQSSPLIQGQAKNTSNNSNGGFPAAASIQEQPNSSCNPAYFPFPSFASIVNGKAGPPAQPGVNGGPQEPPPTWLDACLQHSAFMYEMGPPAATRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMF1</accession>
    <name>A0A8I6YMF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMF1"/>
    <dbReference type="SMR" id="A0A8I6YMF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0432010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0432010.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0432010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519710"/>
    </dbReference>
    <dbReference type="OMA" id="EGHFGSC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF31">
        <property type="entry name" value="OS01G0601700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035179363" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="43"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="890"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="56"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="124"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="323"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519710.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="105295" checksum="DF57771E11052AA5" modified="2022-05-25" version="1">MKMKMKWPVKKTDTIQFHHIHKVTAAAMILLLLASKLPSATSADAKGRSTWGCVAGEREALLSFKESLMDPAGRLSSWRGRDCCRWEGVRCNGSTGHVVKLDLRNTAKDLWSADWMLSGGEMSSSITGLRYLRYLDLSYNDFSATGIPMFIGALDNLRYLNLSCANFQRRIPSHIGNLTKLRYLDVSDDTGTLSTSDLSWLRRLLLLRHLDVSGVFVRSARNWIGHANMLRNLKVLRLSSCGLDSTISTLSPSNLTHLEVLDLSGNAFGTSLQHNWFWGLTSLRELQLSGCDWQAGPIPDALGNMSSLEIIDLAANYQLVGYIPANLEKLCNLQVLNLDWVNINGDLTKLMDRLPKCSWSALRELHLLRANLSGKLPNWIGNLTSLSHLDLYQNMLVGPVPAGIGALGNLASLDFGLSMLSGVLSTEHFASLVSLKYLELSHNSLKLDLNSSWVPPFRLDRGYFRSCDLGPQFPKWLRWQAGITDLDISNTSISDVLPEWFWTVSSHASSLYLSRNQLSGAFPAKLDLVSVEVMDLSKNSLSGKLPANLTAPVLSKLYLSSNHIEGTIPPYVCQLNGLLELDLSSNQLTGDFPRCPENITWRSIGPDFTYCVQFGCSLSILDLKNNKLSGKFPDFLQNSAGLNFLDLSRNLFSGSLPTWLDGKTPNLEVLDLRSNMFCGHLPKHFTNLSRLYYLDVAHNNISGRIPSSLARMKAMTGESEGSKNNYSDNSIMTVTKDQEREYTLDFTNPIVLIDLSCNILTGHIPEKLSFLKGLQTLNLSGNQLDGTIVDGIGALRKLESLDLSYNSLAGEIPSGLSHLTFLSWLNLSYNGLSGRIPSGKQLQTLNYPYIYVGNHGLCGPPLLNNCSNGANPSAHGEHEYASSFSDATSLFLGISMGYVVGLWTVFCILLLSRTWRAAWFRLFDQLCDKSYLQVVVMKAAMVRNFRDEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMX1</accession>
    <name>A0A8I6YMX1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMX1"/>
    <dbReference type="SMR" id="A0A8I6YMX1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0524340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0524340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0524340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0435870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0435870.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0435870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0524340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0524340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0524340"/>
    </dbReference>
    <dbReference type="OMA" id="WSENHIG"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF137">
        <property type="entry name" value="PORR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0524340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="179" mass="20352" checksum="519815133E09F8D3" modified="2022-05-25" version="1">MVTTNLECVQTMVALTEGLGVPRGSRMFRHALSALAFLDEKKIAAKVDYLKKTLGWSDDEVSIALPKAPFLLCKSNESLQHRSEFLISEVGLEPAYIAHRPVMLCYSLEGRMRPRHYVVKFLKENGMLKRDPSYYTVFKETEKYFVEKFICPHKEAAPHLAEDYAAACGGEVPARFRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRP0</accession>
    <name>A0A8I6YRP0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SIAH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123407743</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956878.1">
        <property type="nucleotide sequence ID" value="XM_045100943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650080"/>
    </dbReference>
    <dbReference type="GeneID" id="123407743"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0539520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0539520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0539520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407743"/>
    <dbReference type="OrthoDB" id="675760at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF166">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="6">
        <location>
            <begin position="103"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="285" mass="31608" checksum="D47DE0A2D1C72F34" modified="2022-05-25" version="1">MNGTGMMKQSTKKARTAPVIGGRPDVPRGRGEETVEESANAYTVEADGLGCEICFEPFGDQIFMCKNGHPACGTCCIRMDRICFCAEPIGDIRCRPLEKVLAAMTRPCTYASYGCREIVSYTGRRSHEEDCPYTPYYCPFDGCTYHGMQLYNHIHDRHAASAVVVPTGSRRVAVRLEKKMPFRVLLHGDGARVFLLLNGGEVLSGRSLSLVCMSPRPMGKAEARYKMEVKHWRSAPDTPVLLSSGAAPLVRRLKDFQARGFLFVPDSYWNIGDTVSVTVHLTDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YUE3</accession>
    <name>A0A8I6YUE3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">B-keto acyl reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123411127</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959993.1">
        <property type="nucleotide sequence ID" value="XM_045104058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YUE3"/>
    <dbReference type="SMR" id="A0A8I6YUE3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0674260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0674260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0674260"/>
    </dbReference>
    <dbReference type="GeneID" id="123411127"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0559480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0559480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0559480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0674260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0674260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0674260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411127"/>
    <dbReference type="OrthoDB" id="680872at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000438">
        <property type="entry name" value="Os06g0299100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF38">
        <property type="entry name" value="OS06G0300200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035163562" description="B-keto acyl reductase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0674260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="36914" checksum="7C64C4E8B191803C" modified="2022-05-25" version="1">MAASVWFFFSLALLGALHVSAVSIRLLASLALCLRRPKDLRHRYGAWAVVTGPTSGIGRSMSLELARRGLNLVLVGRDPAKLRDISETISGAHAVQTKTVLFDFSLVSAAQGDEAMRRLREAVAGLDVGLLVNNAGVAKPGAMYLHEADVEAWARMIRVNVLALTEVTAAVLPGMVRRGRGAVVNLGSGSASVLPSFPLYSVYAATKRYVAEFSRSLAVEYGSSGIDVQCQVPFLVETNMVSSAVKANLILQFVLTPDAYAYAAVRWIGHGRLCVPNVAHRFQGWYAGLFPDFVNDAFRLEKNRGQRAILRRVKPWRKSQSQGHSRVPIQDKGGVGVRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YV94</accession>
    <name>A0A8I6YV94_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase LOC123452243</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123452243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM5 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044984798.1">
        <property type="nucleotide sequence ID" value="XM_045128863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YV94"/>
    <dbReference type="SMR" id="A0A8I6YV94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510770"/>
    </dbReference>
    <dbReference type="GeneID" id="123452243"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0424270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0424270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0424270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0510770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452243"/>
    <dbReference type="OrthoDB" id="206354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03187">
        <property type="entry name" value="Methyltr_EFM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019369">
        <property type="entry name" value="Efm5/EEF1AKMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200:SF0">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="247" mass="27267" checksum="132767DE4B45F135" modified="2022-05-25" version="1">MASGAEEEGRARREAGEEDTDEDDERPQLSAAAAGALREFLEEQGRHEGEKGGGEGVELVAEDWRLSQFWYDERTARELSEEVARLASGLPAGASTSAAVACVACPTLYAYLRKSSPDVPARLLEYDERFGQYGDDFAFYDYNQPEALPPAMKHAFQIVVADPPYLSQECLEKVAKTVSFLAQPEGSFLLLLTGEVQKDRALELLNVRPCGFKPQHSNKLGNVFRLFTNYDPVDRLGGWDRSDGAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZY5</accession>
    <name>A0A8I6YZY5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123407262</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956295.1">
        <property type="nucleotide sequence ID" value="XM_045100360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZY5"/>
    <dbReference type="SMR" id="A0A8I6YZY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636950"/>
    </dbReference>
    <dbReference type="GeneID" id="123407262"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0636950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407262"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00574">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="273"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="275"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="477" mass="53150" checksum="8F9DD29BB47FB9C6" modified="2022-05-25" version="1">MMASHEPHHHLLGFSSSSPVTPNPAAMAMAFDHHGLFAPLQEDMPPRSGGDRPWPPHVSMLSLDNTASQHELQPFTVAPPSMMTMQQQSFWLNSSRYLGPARELLSEFCNLEGDAMNRGATMQAPKLDSDKSPACGPWGANPSVSSMDYMALERRKTRLLSMVEEVDRCYRRYREKMRATEMSFDEVAGMGAAQVYTKLAMRAMSRHFRCLRDALVGQIRTLKKSMGESRDADGMLAAPGASKGDTPRLRVVDQCLRRQRAFQQYGGAAAIESCPWRPQRGLPERAVAVLRSWLFEHFLHPYPNDVDKHILARQSGLSRSQVSNWFINARVRLWKPMIEEMYAEETIQHDDNGASDGRAEPSPADHQKNKLAAWTKVATIRDESCHRFSSTNNPSGCFIPSSLVAEGGELRGYPCLRSDVGNGAVSLTLGLQQQRAFTSPAMIMQQQSSLIIGAEEEDVVLPYRNLMGSELLHDFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B278</accession>
    <name>A0A8I7B278_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123424763</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPX_BAH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="BAH1"/>
    </dbReference>
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        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46764">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BAH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46764:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
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    </dbReference>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
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        <location>
            <begin position="226"/>
            <end position="275"/>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0113540.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="330" mass="37479" checksum="CF7806A969D89F72" modified="2022-05-25" version="1">MKFTKRYETYMRGMRTAAEQQLPAVGLKRLKKMLKKCRSHHAPHHKTSSADAGASSARCHGHCSVCDGSFFPSLLDEMSAVVGCFNEKAKKLLELHLASGFKKYVMWFSNRGHKGHGKLIQQGKDLVTYAIINAVAMRKILKKYDKVHYSKQGQEFKAQAQSLHIEILQSPWLSELMAFYMNLRRSKNNEAAMELFGDCSLTFDDEQPTLSCNLFDSMRVDISLTCSICLDTMFDPVSLSCGHIFCYLCCCSAASVTIVDGLKSADHRSKCPLCRQAGVFPNAVHLDELNMLLSYSCPEYWEKRMQMERVERVRLAKEHWESQCRAFLGV</sequence>
</entry>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
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        <location>
            <begin position="17"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8519" checksum="C627BF51E867FF57" modified="2022-05-25" version="1">MGIHLLAFAFVQVFQASAPLLWPLNLWLPRHLPQVCAVVSGEIAAHVAWLRRAHARRRDDDEEVRRESLLYAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5L0</accession>
    <name>A0A8I7B5L0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">65-kDa microtubule-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444136</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976692.1">
        <property type="nucleotide sequence ID" value="XM_045120757.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280910"/>
    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280910.1"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="65-kDa microtubule-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF7">
        <property type="entry name" value="65-KDA MICROTUBULE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAP65_ASE1"/>
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    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="495"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="591"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="156"/>
            <end position="183"/>
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        <location>
            <begin position="464"/>
            <end position="494"/>
        </location>
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        <location>
            <begin position="501"/>
            <end position="512"/>
        </location>
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        <location>
            <begin position="601"/>
            <end position="612"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280910.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="78422" checksum="7EA4C7C8A2E75869" modified="2022-05-25" version="1">MAMGGAVKDQQQQLHPCDSLLLELNVIWDEVGEPDSVRDKTLLELEQECLDVYRRKVDQANRCRAQLRQSIAEAEAELAGICSAIGEPPVHIRQSNQNLHGLREELNAIIPYLEEMRTKKVERWNQFVRVLEEIKKISSEIRPADFVPFKTPVDQSDLSLRKFEELTKELESLQKEKRERLQQVMDHLNTLHSICEVLGIDFKQTVHEVHPSLDEAEGSKNLSNTTIERLASAVNRLREIKIQRMQKLQDFASTMLELWNLMDTPIEEQQMFQNVTCNIAASEHEITEPNTLSIDFLSYVEAEVLRLEQLKGSKMKDLVLKKKSELEEHRRRAHLIGEDGYSDEFNIEAIESGAIDPALVLEQLEAHIATVKDEAFSRKDILEKVERFLNACEEEAWLEDYNKDDNRYNAGKGAHLTLKRAEKARILVNKIPGMVDVLTTKIIAWENERGKEFTYDGVRLLSTLDEYMIVRQEKEQEKKRQRDQKKLHDQLKAEQEALYGSKPSPSKPQSIKKAPRHSMGGVNRRVSIGGATMQAPKTDILHSKNVRAAKRTEDIAHLSPASRGLDIGDLPIKKLSFNATALRETETPRKPFAQIMPPASSVPSTPARSVTNDTEDENRTPNPKTYGAALNLKTPTVAAPMQLAMTPTVANKVTAAPVSLVYEKPEPALPEAVEYSFEEKRLAVYLSREVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5Y8</accession>
    <name>A0A8I7B5Y8_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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            <end position="92"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0155780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="269" mass="30241" checksum="61771D2406BE2A7B" modified="2022-05-25" version="1">MKWQSFMSTFVLNKMCDLISSGVRTDKGFKEVHLNTVAKQVFEFCGQDVSATQVYNHLRKWRDRWIKVSKLRDLRGASWDENTCSIVLEAEHYDGYVADHPRDAEFLNTPIQNYNQMQHIFSFGLTTGKHAMGSGDPLGSPMPDFPATPKVEVIDGPDKPFEKPFDKPFDPIHGRKRKRGGLMEEEINVFCSMTEAVKEVATAIMVCKPLDVHPDLYGAVMTEGGFSDDALMAALSHLLDNKAQAVGFVAMANAHKVLWLGSWLGKHNY</sequence>
</entry>
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    <accession>A0A8I7B6I6</accession>
    <name>A0A8I7B6I6_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0326030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0326030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0326030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441084"/>
    <dbReference type="OrthoDB" id="1424615at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
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        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="133"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0326030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="48849" checksum="822C8F8012AE896F" modified="2022-05-25" version="1">MLGQFNLDFETMSTCKKARAEATSIVSSDRLSSLPPEIKGDILSRLNVEEAVRTSILSTTWRDEWTDMPEISLCDGNFTRTKFITLVDMVLLLHKGIIEEFDISGNKSYHDEFGRWMLMLSRRSPKSVIIKLNSGPRYRIPSYLFSIDGLMSLELENCIISLPLVFQGFKSLTDLSLKNFYSTDMDIQTLISSCPDLTDLILTSVEGIDCLNIQAPKLEYLNVEGEFEDIKLDAPNLEVAIFSLDQKAKAYQSVPIAHDKEGYVKQLLESISDVKTLSVSGSLFLKYLIKGCILTKFPAMFHRLENIYLGICIWDQRQASAICSFFQNAPNLKMVELWSYPRITWEHYQDQAIIQELTLEMQMDHLVTANVKHFGGLDYEVDFVAKLLSWAPALEEVKLEWKGKIDRSMVLTKLLALPRVSPRAKIIIT</sequence>
</entry>
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    <accession>A0A8I7B8S6</accession>
    <name>A0A8I7B8S6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8S6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0468090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="17866" checksum="36584548669D4FD5" modified="2022-05-25" version="1">MARSTSYSHVEAVLPLIPCPRCQSRIIMFVSHRGERFYKCVNKDAGLCSFLRSTDTYLSELTRADFSQPQVMHHPVAVHQSAPLQHRSTQVQDAMDGSQAEIQPSHVRVRPQVHQAPPLPVAATHCCSNDTQRTVLQLARTNLIIVVSFLYLIFGQQCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8U8</accession>
    <name>A0A8I7B8U8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8U8"/>
    <dbReference type="SMR" id="A0A8I7B8U8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0220700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0220700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0220700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0220700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0220700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0220700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035315600" description="Protein kinase domain-containing protein" evidence="13">
        <location>
            <begin position="27"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="333"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0220700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="644" mass="72022" checksum="4FDD61E20E098224" modified="2022-05-25" version="1">MAMPTTLKVLTILYVLVLLVAYQVEGRNHRPDCPTFSCGPLGYVSSPFRQASDPPGCGHQSYELVCSDTKATIHIDNATYYVSGINYSDSTFWIVDADIDLYNSCPLPRWNYDQHPAAGFTLRNRQHDTHHAIKVELAPAAYNQAIFVNCSREVKDNGRGSTMANLEPCCGYLAMTPLGGQEIGMKGTAPLENASYADVVKFMTHGFRVKFPFRLRQSGDFLGCLMDDLAPTFTKDWILTILSIDVSFMGCAAKAVPEPFDICLYIIMASLAYARLIAVFWRFVLVPLVVLIFLAHKYWKTRITMDAVEKFLQMQQMIGPSRYAYTDITAVTSHFRDKLGQGGYGSVYKGVLLPGNVHVGVKMLEGNSNCNGEDFISEVSTIGRIHHVNVVRLVGFCSEEMRRALVYEYMPRGSLDKYIFSAEKTFSWDKLNEIALGIARGIDYLHQGCEMQILHFDIKPHNILLDSNFIPKVADFGLAKLYPRDKSFVPSRALRGTIGYIAPEMISRSFGVISSKSDVYSFGMLLLEMAGGRRNADPNAANTSQAYYPSWVYDKLTAPVVDAICPVASMHELERKLCIVGLWCIQMKSHDRPTMSEVIDMLEGGFDGLQMPSRPFFCDDDHTAVPDSYPLLSELTEISEEDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BC24</accession>
    <name>A0A8I7BC24_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calmodulin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978917.1">
        <property type="nucleotide sequence ID" value="XM_045122982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC24"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362450"/>
    </dbReference>
    <dbReference type="GeneID" id="123446317"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0362450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446317"/>
    <dbReference type="OMA" id="REPYHHA"/>
    <dbReference type="OrthoDB" id="667051at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012442">
        <property type="entry name" value="DUF1645_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095:SF51">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07816">
        <property type="entry name" value="DUF1645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362450.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="33869" checksum="A094183877E0FC06" modified="2022-05-25" version="1">MTAAADDDAPSDDPLAGGGDSACSTPFVSAPSSPTRDPFSGHHHAACFFSAPASPTRGASNEFACGGLEFDFDFDFSSRFPSSSAAAMSSADELFHNGQIRPVRLSAMLLQPHQPHLASPSQAPVEVEEGERGRFWGRSVHHKARSLSPFRAHWRSPSPAPPPESESVQPAATPPASRSSSSSSTASSASSSSSRSSRRWGFIKDLLHRSKSDGGKDNQHPAAPAPPTFPATPKSSPSPSPSPAAGRSRETGRGRRRSAHERLYEARRAEAEEMRRRTYLPYRQGGLLLFGCIGLGNRSGTAVHGLARGLNTATAVSSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2D076</accession>
    <name>F2D076_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402214</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">cv. Morex</strain>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable membrane-associated metalloprotease that may be involved in chloroplast development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="AK360268">
        <property type="protein sequence ID" value="BAJ91477.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980204.1">
        <property type="nucleotide sequence ID" value="XM_045124269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D8Q6"/>
    <dbReference type="STRING" id="112509.F2D8Q6"/>
    <dbReference type="PaxDb" id="4513-MLOC_78893.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339340"/>
    </dbReference>
    <dbReference type="GeneID" id="123447650"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0283080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0283080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0283080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0339340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339340"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ7R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032693_0_0_1"/>
    <dbReference type="OMA" id="MPRVIRI"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D8Q6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06160">
        <property type="entry name" value="S2P-M50_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF2">
        <property type="entry name" value="ZINC METALLOPEPTIDASE EGY3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="546"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="53"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="133"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ91477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="582" mass="62827" checksum="8E6482B83EC9B2A0" modified="2011-05-31" version="1">MASTSLVASIVCSSSGSSSTGRSAALPRSFCRNHHRLSLRPLRSSSIVRCSLQKQDQAKENRPVSSIAVAPDEQKVEESKKASSHHAGGGGGGDHPEGGDGEDSEKKSRDEQQEVDWRSDEEFKKFMGNPSIEAAIKLEKKRADRKLRELDREPDASPISGLLRGFIKGTLEREKQRLEEAEQTFKALDLNKLKSCFGYDTFFAVDVRRFGDGGIFIGNLRKPIEEVRSKLEKKIAETAGTEVTLWFMEERNEDITKQVCMVQPKAEIELQLEMTKLSTPWGYLSAVALAVTTFGTIALMSGFFLKPGASFDDYVSDVLPLFGGFLSILGVSEIATRLTAARYGVKLSPSFLVPSNWTGCLGVMNNYESLLPNKKALFDIPVARTASAYLTSVALAVSAFIADGSFNGGENALFIRPEFFYNNPLLSFVQQVIGPYTDELGNVLPNAVEGVGVPVDPLAFAGLLGIVVTSLNLLPIGRLEGGRVAQALFGRRTAALLSFGTSLLLGAGAVGGSVLCLAWGLFATFIRGGEEIPAQDEITPLGSERYAWGFVLAVVCLLTLFPNGGGTYSSSFLGEPFFRGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2DBT2</accession>
    <name>F2DBT2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449037</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK361346">
        <property type="protein sequence ID" value="BAJ92553.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982042.1">
        <property type="nucleotide sequence ID" value="XM_045126107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DBT2"/>
    <dbReference type="SMR" id="F2DBT2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449037"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0068230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0068230.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0068230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449037"/>
    <dbReference type="OrthoDB" id="642765at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF181">
        <property type="entry name" value="OS02G0764700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="127"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ92553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="259" mass="28415" checksum="664B41C5E0ACFE6E" modified="2011-05-31" version="1">MTFSLSAAMEGGHQEYMIRFDDPSPSTATAEPPPPEPFAGRAITPDQEHSAMVAALLHVISGYTTPPPDLFPTREVCPVCRVDGCLGCDFFGAAEAEATGTRQIAATLDAPKAAGGPQRRRRNKKNKYRGVRQRPWGKWAAEIRDPRRAVRVWLGTFDTAEDAARAYDRAAVEFRGPRAKLNFPFPEQQQQLGDGNAAAAKSDTCSPSPRSADAEVRVPQSWQQNGGQEAGDQLWDGLQDLMKLDESELWFPPSSNSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDP0</accession>
    <name>F2DDP0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123425706</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362007">
        <property type="protein sequence ID" value="BAJ93211.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965354.1">
        <property type="nucleotide sequence ID" value="XM_045109419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDP0"/>
    <dbReference type="SMR" id="F2DDP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135240"/>
    </dbReference>
    <dbReference type="GeneID" id="123425706"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0111460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0111460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0111460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0135240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425706"/>
    <dbReference type="HOGENOM" id="CLU_024029_0_0_1"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054296">
        <property type="entry name" value="DUF7032"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043:SF5">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23005">
        <property type="entry name" value="DUF7032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7032" evidence="3">
        <location>
            <begin position="26"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="333"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ93211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="588" mass="62918" checksum="058BA6B04045DB89" modified="2011-05-31" version="1">MGEFAAAGEAGSQEAEQDEQGRLATALDAINSLISASFSASLFPLKWQLVRDRFNRLHAGLADITVIAASDGGERHEAFEGLLRDVVDAVREARELVPRSQGRHYGGGKLRLRSDLDVVASTLDTHLARLDEICASGSLTRARALVVPRPCAGASREDVRFYVRDLFARLRVGGAEMRREAAAALNEVLRDDDKCVRLVVSDVADGIGVLVGLLESPDACIQEEALDAISVIAGHDACKGDLVVGGVIAPVIRVLNTGAGPAAKERAARVLSKLTENADNAWAVAAHGGVTALVNICSDHRASGGELVCAACRVLKSLVGVEEIRKYMVADAGAVPVLVSLLQGPAEEGAQIQAMELLAAIASGDSSSREVVLQEGTAESLVRALDPGIPRSSKAREVALRAIDALCFSSPDSIDRLIGAVFLNRVLFFLRNGDTTLQHCALKAAHRLCHVSEETKKAMGDAGFMPELVGIVQAAKSLETREMAAEALSAMMSVHRNRKRFVQDDRNVAQILQLLGPDEEKPSPAKRFLLSTLVHLADSNSGRRKIMSSEHVRNLEKLAETNVTDAKKIVKKLGGSKLRNMFHGIWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWC3</accession>
    <name>F2DWC3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123445053</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368191">
        <property type="protein sequence ID" value="BAJ99394.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977912.1">
        <property type="nucleotide sequence ID" value="XM_045121977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWC3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305540"/>
    </dbReference>
    <dbReference type="GeneID" id="123445053"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0255140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0255140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0255140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0305540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445053"/>
    <dbReference type="HOGENOM" id="CLU_105206_0_0_1"/>
    <dbReference type="OrthoDB" id="1918709at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012881">
        <property type="entry name" value="DUF1685"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31865:SF75">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31865">
        <property type="entry name" value="OSJNBA0071G03.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07939">
        <property type="entry name" value="DUF1685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ99394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="170" mass="17935" checksum="290C5A317567F83B" modified="2011-05-31" version="1">MAAISSTSYFSSQPQLPASDGASPATTRRSSSSNGRQPRRRRAGRCVMLEADAASGGGGVGGGAVAGRARSLTEEDLEELKGCLDLGFGFSYHEIPGLRGTLPGLELCYSMTRRFLDEQRTLVGQLEPAAAAAAPIPDWKISGPGDDPDEVKARLKYWAQTVACTVKLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F2E915</accession>
    <name>F2E915_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123428071</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK372640">
        <property type="protein sequence ID" value="BAK03837.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968170.1">
        <property type="nucleotide sequence ID" value="XM_045112235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E915"/>
    <dbReference type="SMR" id="F2E915"/>
    <dbReference type="STRING" id="112509.F2E915"/>
    <dbReference type="PaxDb" id="4513-MLOC_6261.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202500"/>
    </dbReference>
    <dbReference type="GeneID" id="123428071"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0168330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0168330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0168330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0202500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428071"/>
    <dbReference type="eggNOG" id="ENOG502QTUY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038016_1_0_1"/>
    <dbReference type="OMA" id="MGMEDNV"/>
    <dbReference type="OrthoDB" id="10264870at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22933">
        <property type="entry name" value="HFD_HFI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024738">
        <property type="entry name" value="Hfi1/Tada1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12767">
        <property type="entry name" value="SAGA-Tad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK03837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="427" mass="46740" checksum="8A9408649606F47E" modified="2011-05-31" version="1">MDTVRGEAWSMAPPQQQRQPSGPRGPQPPPQQQNGRVDLRELKAQMEKRLGPDRSRRYFGYLSGYLSQKLSKPDFDKMCLLTLGRENLRLHNRLIRAVLYNVYQAQCPPPPPDAGRSVGPSAKKVSHAAGSLNSCNGDARLLQVQGSRSMGALQDHQLKDRLKHMGPNGRVEAAASHTQVVHGGSAVTENGALSSLELKRSVSFQQCEAAEPMAKHLRVDQLLPENALRQRRIMSDAAGHSAQMSKSPVRAPLGIPFCSASVGGSRKLLPPAVNAGEDHFSSCYEQGQLLNTEVLRKRMEKTAETLGLAGVTMDCAELLNNGLDSYLKNLIRSSVELKGADVRRDARKGASYKQQAHGKQINGVLLPNQVQMQSSSGQSDATNDGRSQHLISSHDFSVAMQLNPQQLGEDWPVLLEKICLRPSEEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2EBZ1</accession>
    <name>F2EBZ1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123395841</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cellular auxin homeostasis by regulating auxin metabolism. Regulates intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the auxin efflux carrier (TC 2.A.69.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373666">
        <property type="protein sequence ID" value="BAK04863.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946811.1">
        <property type="nucleotide sequence ID" value="XM_045090876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EBZ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491050"/>
    </dbReference>
    <dbReference type="GeneID" id="123395841"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0407040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0407040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0407040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0491050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395841"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080162">
        <property type="term" value="P:endoplasmic reticulum to cytosol auxin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045033">
        <property type="entry name" value="PILS1/3/4/5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651:SF33">
        <property type="entry name" value="PROTEIN PIN-LIKES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK04863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="411" mass="43761" checksum="6E45A8FBF68AE60E" modified="2011-05-31" version="1">MGLLELFVTACMPVLNMLLVTGVGSFLATDSAGILGKEARKHLNYVVFYVFNPSLISTYLAKTITMESLAKLWFMPVNVLFTFTFGLIFGWIVIKVTRAPLKLRGLILGCCSAGNLGNIFLIIIPALCKEKGSPFGAPDVCQTYGLAYSSLSLAIGAVFLWTGAYNIIRANSNVTEEDGNSPITQTKVLVSGSTISAVSEDKHSISSDRVDECALLLISNRTKTKVPLLERAKGFVSSVSGAVDLKKLFAPSTIGVIVGFIIGGTPLIRNALIGDDAPLRVLRDSAELIGGAAVPSVTLIMGGNLITGLRGRASVPPSVIAGVVVVRYVLLPLVGTVLVKAAVRYGVIRPDPLYQFVLLLQHAVPPAMNIGTITQLFGVGESECSVIFVWVYALASVAVTVWSAFFMWTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2EEW6</accession>
    <name>F2EEW6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123452699</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM97/sigma-2 receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374692">
        <property type="protein sequence ID" value="BAK05888.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985342.1">
        <property type="nucleotide sequence ID" value="XM_045129407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EEW6"/>
    <dbReference type="SMR" id="F2EEW6"/>
    <dbReference type="PaxDb" id="4513-MLOC_21276.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519010"/>
    </dbReference>
    <dbReference type="GeneID" id="123452699"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519010"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452699"/>
    <dbReference type="eggNOG" id="ENOG502RZ62">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086812_0_1_1"/>
    <dbReference type="OrthoDB" id="433124at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EEW6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051987">
        <property type="entry name" value="Sigma-2_receptor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016964">
        <property type="entry name" value="Sigma2_recept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204:SF1">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031032">
        <property type="entry name" value="TMP_97_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK05888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="162" mass="17186" checksum="E649AAC333ED40B0" modified="2011-05-31" version="1">MGVVSAVTDAVVVLFSLTIAVAVPLIDAQAVLPPDLYPAPLRDLKRWYAAEFGDYLMAQPPAFLHGLFCLELAFLWPLSVATIYGVLAGRRWAATTSLMAGVTTLTSMSAILGEMLGSGKATPKLLQMYVPYAVFAVIAILRGLCSCSAPPSPASSARKKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2EGD3</accession>
    <name>F2EGD3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123396003</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK375210">
        <property type="protein sequence ID" value="BAK06405.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946964.1">
        <property type="nucleotide sequence ID" value="XM_045091029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EGD3"/>
    <dbReference type="SMR" id="F2EGD3"/>
    <dbReference type="STRING" id="112509.F2EGD3"/>
    <dbReference type="PaxDb" id="4513-MLOC_75604.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441930"/>
    </dbReference>
    <dbReference type="GeneID" id="123396003"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0365880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0365880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0365880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396003"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043181_2_0_1"/>
    <dbReference type="OMA" id="CQPAMVN"/>
    <dbReference type="OrthoDB" id="745641at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000007">
        <property type="entry name" value="Osmotin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090939" evidence="3">
        <location>
            <begin position="28"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="90"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK06405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="240" mass="24639" checksum="8D2ED06EF22584D1" modified="2011-05-31" version="1">MAAMAERLVIGTLLAAAFLASSASASAADTTRLTLHNLCPYPVWPLVTPNTGFPAICDNAVRLEGNGHGLVSFTFPPTFWAGRLVARTGCTSPSRCETGNARPVGVVQLAVHSAEGGGHRQQDLAVYSVSLVDGFNVPAVVSPQSIGGDGPCPALGCAADLNAGCPPAQRVVGAGGRVVACKGPSGYFKQRCPLTRTTPIDREPVPQNCHAPGELKVVFCQPAMVDADAAAQPEIGVAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2EH99</accession>
    <name>F2EH99_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123401793</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375526">
        <property type="protein sequence ID" value="BAK06721.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951577.1">
        <property type="nucleotide sequence ID" value="XM_045095642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EH99"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607360"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
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    </dbReference>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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            <begin position="85"/>
            <end position="107"/>
        </location>
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            <begin position="119"/>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">Predicted protein</fullName>
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        <name evidence="2" type="primary">LOC123430116</name>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Triticodae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="OS07G0115500 PROTEIN"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </source>
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        </source>
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    </evidence>
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</entry>
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        </lineage>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045090769.1"/>
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        <property type="nucleotide sequence ID" value="XM_045090770.1"/>
    </dbReference>
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    <dbReference type="STRING" id="112509.M0V9E2"/>
    <dbReference type="PaxDb" id="4513-MLOC_20816.3"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440860.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123395740"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123395740"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="P:asymmetric cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="POLAR-like"/>
    </dbReference>
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        <property type="entry name" value="EMB|CAB62613.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33476:SF7">
        <property type="entry name" value="EMB|CAB62613.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="109"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="440"/>
        </location>
    </feature>
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            <begin position="580"/>
            <end position="612"/>
        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
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        <location>
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            <end position="434"/>
        </location>
    </feature>
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        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="641" mass="71019" checksum="F857A9633576ADF8" modified="2013-04-03" version="1">MDKQKNHKNAEMGNDVFRRMILNQVGRDIGLEEENVPCNTPRNSVHSRFGGSSARFAASTNGSTGAESVSPGEYVRDPGSILSLQPWIFKRSSTKDNEEMAVPSGRRAVGRGKNLVDGFRDGSTTEVSTRSPGLGSGPGGGRGALRSRRPQRHFFKPLVATENSYIPQLYNENFEFEECTFAPVPSPASARPFIVTDGRRIISKSRYEPVPVPFNIGFDKEGCRNGSEVPESVVGIAPLPELNKSKRDSRVAQGTRFSLPGSQSERYSKPSKSAGLLERMLMFSSGVGIGIISSSLSSKKDLDSLKGTLKRMENLVQDLQDELEMKEGLTVEELPNETSGEQDDSNIKAHTPDSMSMSKIEAELEAELARLELNITSNHLEEEPFDLSEIDQEFIGDIVQGELKSDMIRRDIADYSSDSDHGRDSRRSSSDYTHETNYPVSPRDLSLRLQKVIQHRLEDRIKELETALAQSQKQAQLHIMARQRVFSERTCSNSDSGSSSNQDSPLLIQEASSSAEPFCLNLAGDALEAYDEAYEEFMRIADSPCTTSTNGRPQVNEDYSVDRSLIWGLEDESATELKEDSTWEQAVNIGDPNRAQESDGDESGDEDDHDSEMLIQQIVERTKQGSPVLIHAQRMLFSVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>M0VJR7</accession>
    <name>M0VJR7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123452206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045128817.1"/>
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    <dbReference type="PaxDb" id="4513-MLOC_31648.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0439690.1">
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0439690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="473" mass="54516" checksum="5165794B07F0D508" modified="2013-04-03" version="1">MDCPKRSASADLPEDSLVEVLARVPARSVYRSKCVAKAWRNLIEDPLHRKKLPQTLQGFFFIGKETHRQRVGFTNLLARSVPPQIDPSLSFLKKLPGIGTLSFLDSCNGLLLFKHKKKPRPYKLLGYVVCNPATKQWAEVPRYGPPDPEYARSPDRYSYLVFDPTVSSHFHLVQFGWEFAELVKIEEEGFMVDEDEDGDGDDEGNDDYVPDHWKHDGYVPDYWELRHQQRYNPNIYIPDYIHSDDDNDCDEENEDDGGDVSRTSVQVYSSETGKWTHMQSDWSPIQSDWDKHDLEGWRLQGSELESSCCAVLNSMLHFTILDQIVVVDVQGVTRKIITVPKMAERSHRPNPGYIAQSQGRLHYINQASDAELSIWVLENYDTQDWVLKHTVSFVELFTKKSCTVDKNHYNVVAMHPDGNVVFIVLYRNQKLISYDMDHKLVSIIRTFRNEPPDMHVVPYVPCFSESPALPNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0XMJ6</accession>
    <name>M0XMJ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TPX2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442377</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPX2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974328.1">
        <property type="nucleotide sequence ID" value="XM_045118393.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974329.1">
        <property type="nucleotide sequence ID" value="XM_045118394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XMJ6"/>
    <dbReference type="SMR" id="M0XMJ6"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219790"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219790.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219790.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219790"/>
    </dbReference>
    <dbReference type="GeneID" id="123442377"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0183040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0183040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0183040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219790.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219790.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442377"/>
    <dbReference type="eggNOG" id="ENOG502RERJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1925970at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XMJ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044806">
        <property type="entry name" value="WVD2/WDL1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46372:SF4">
        <property type="entry name" value="OS11G0592600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46372">
        <property type="entry name" value="PROTEIN WVD2-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 C-terminal" evidence="8">
        <location>
            <begin position="236"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="290"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="243"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="43158" checksum="9BDAE82E71FB6AEC" modified="2013-04-03" version="1">MGIEVVLQSSKEELELDSSLIPPSSDNMETPDDKGDHITELSGTVNTESSVGARSKNGVSEPLEAGDGNGDTEHDGSSATGHAEQTDDEPAADEASPVNVEKGDVAQHGVESRAYEQENQEKFPATETAGSDSTSVTSMEDVVHPKEGAQSEPGDDSGYHPDLPNDKHSTGNGNVYENAKCVLTSSTKKMKRSASATTRKPLQATNRNSPDDWNASTLTNSKSSNGKTITVPSSPVFRCTERAEKRREYYSKLEEKHQAMEEQRIQLEARLKREQEEALRLLRKSLTFKATPIPSFYHEAPSPKAEYKKLPTTRPKSPKLGRKKAAMNTSHSSEESESTRPCCRASRDRHDSQCKCHNSSSKPRQHPQQHPAASSSKKQAKNHAHKLSALNIAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y1Q1</accession>
    <name>M0Y1Q1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123442794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974870.1">
        <property type="nucleotide sequence ID" value="XM_045118935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y1Q1"/>
    <dbReference type="SMR" id="M0Y1Q1"/>
    <dbReference type="FunCoup" id="M0Y1Q1">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0Y1Q1"/>
    <dbReference type="PaxDb" id="4513-MLOC_64958.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233650"/>
    </dbReference>
    <dbReference type="GeneID" id="123442794"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442794"/>
    <dbReference type="eggNOG" id="KOG1843">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Y1Q1"/>
    <dbReference type="OMA" id="VAPCYSY"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y1Q1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11526">
        <property type="entry name" value="SYLF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000151">
        <property type="entry name" value="Zinc finger family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="493" mass="53069" checksum="3200CC974828CDFD" modified="2013-04-03" version="1">MTNSSEVPFYHSLPVKGAESSLKDQIHSKVMGTIGNVINSIDPKSLPQQAWETAGNAVNSLESKLSGQKPFDFDGGNDFLDGYECPDDYWDPELQRAEKPVNIRNLLGGIVAIIGRTCKNDEIQQSKDTKSSVSFLGSSSDGDTFLHTSVYAPSAPPLLDEEALSYNIYRVVLEAEPPEWLPDSYADSCMQCSASFTAITCGRHHCRFCGGIFCRACSKGRCLLPAKFRERNPQRVCDACYDRLDPLQNLLINSVSNATQTAKHDVMDWTSARGWLNLPIGLTLEHEIYKAAVSLRSYSQVSRINPGKSIPHAVLSGASGLAILTVVKAGAFLTYKLGTGLVVARRSDGSWSPPSAIASAGLGWGAQVGGELMDFVIVLHGPEAVKTFSSRMHFSIGAGLSAAAGPVGRVLEADVRAGNKGSGVCYTYSCSKGAFIGVSLEGNIVSTRMDANLRFYGDPYLTTSDILMGNLDQPNAAKFLYKALDDLYSGLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>M0YKN0</accession>
    <name>M0YKN0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPI-anchored protein LLG1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123440983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973454.1">
        <property type="nucleotide sequence ID" value="XM_045117519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YKN0"/>
    <dbReference type="SMR" id="M0YKN0"/>
    <dbReference type="STRING" id="112509.M0YKN0"/>
    <dbReference type="PaxDb" id="4513-MLOC_70286.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321860"/>
    </dbReference>
    <dbReference type="GeneID" id="123440983"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440983"/>
    <dbReference type="eggNOG" id="ENOG502S17V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YKN0"/>
    <dbReference type="OMA" id="ANECKET"/>
    <dbReference type="OrthoDB" id="585255at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YKN0">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058888">
        <property type="entry name" value="LLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039307">
        <property type="entry name" value="LORELEI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31533:SF16">
        <property type="entry name" value="GPI-ANCHORED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31533">
        <property type="entry name" value="GPI-ANCHORED PROTEIN LLG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26578">
        <property type="entry name" value="LLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GPI-anchored protein LLG1-like" evidence="1">
        <location>
            <begin position="54"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="18048" checksum="22F5020FC0489203" modified="2013-04-03" version="1">MALTRLFIFLFLAAVLARLASSSASPFMSDIVYQASTGSTGRSLLQTKNDCPISFEIQNYTILTSKCKAPYYPPLECCDAFKEFACPFAVYINNQSTNCGDTMFSYIIIRGYPKGLFADECLNGKEGVSCEGIPEIGTDVPSGGRLVQGVSSYLVLLLCGLGALLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0Z1G0</accession>
    <name>M0Z1G0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123401519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951278.1">
        <property type="nucleotide sequence ID" value="XM_045095343.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951279.1">
        <property type="nucleotide sequence ID" value="XM_045095344.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951280.1">
        <property type="nucleotide sequence ID" value="XM_045095345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Z1G0"/>
    <dbReference type="SMR" id="M0Z1G0"/>
    <dbReference type="FunCoup" id="M0Z1G0">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0Z1G0"/>
    <dbReference type="PaxDb" id="4513-MLOC_75433.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0544420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0544420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0544420"/>
    </dbReference>
    <dbReference type="GeneID" id="123401519"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0452860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0452860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0452860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0544420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0544420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0544420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401519"/>
    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Z1G0"/>
    <dbReference type="OMA" id="CYFACEA"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Z1G0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd22152">
        <property type="entry name" value="F-box_AtAFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF26">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0544420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="39249" checksum="D02EF43EBA91DE58" modified="2013-04-03" version="1">MESADIQTQLIHGLPDEVALLCLARVPRQYHNALRRVSRGWKALLCSEEWHSYRKRNNLDESWIYVICRGTGFKCYVLVPDPTTRSLKVIQVMEPPCSRREGVSIETLDRRLFLMGGCSCLKDANDEVYCYDAASNHWSIAAPMPTARCYFVSASLNDKIYVTGGFGLTDKSPNSWDIYDKATDSWRSHKNPMLTPDIVKFVALDDELVTIHKASWNRMYFAGIYDPVDQTWRGKENEIALCWSGPTVVVEGTLYMLDQSLGTKLMMWINETKEWVMVGRLSDKLTRPPCELVAIGRKIYVIGRGLSTVTIDMDTAARVDGFLVSSSTAPLMEHDFPPEKCRVITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>M0ZD42</accession>
    <name>M0ZD42_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase subfamily 4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123449612</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044982828.1">
        <property type="nucleotide sequence ID" value="XM_045126893.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982829.1">
        <property type="nucleotide sequence ID" value="XM_045126894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0ZD42"/>
    <dbReference type="PaxDb" id="4513-MLOC_81878.9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405200"/>
    </dbReference>
    <dbReference type="GeneID" id="123449612"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0337320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0337320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0337320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0405200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449612"/>
    <dbReference type="eggNOG" id="KOG2941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CKLIIDW"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0ZD42">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000109">
        <property type="entry name" value="Chitobiosyldiphosphodolichol beta-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000083">
        <property type="entry name" value="UDP-glycosyltransferase TURAN isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="10">
        <location>
            <begin position="37"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="473" mass="52250" checksum="C5BC420C8D6DBC9A" modified="2013-04-03" version="1">MVMAEEAGRRKRAAVVVLGDIGRSPRMQYHSLSLANQAGMEVDIVANGGSDPHLSLRENPSIHIHEMKSVQLSGISKISGALALLLKAAIQFIMLIWFLCFKIPRPDVFIVQNPPSVPTLAAVKLASWLRGAKFIVDWHNFGYTLLGLSHGRSHVIVKVYFWFEKHFGRMADGAFCVTKAMQHELAQNWGIKATVLYDHSPEFFHPASLTEKHELFCRLGSSICGAMGSADCISVEKEVEDKNTTVLTSKIDGGVSLKPNRPALVVSSTSWTPDEDFSILLEAALMYDRRVAATLGEEDSMDEGQLWIDIKNGKQFDYPRLLFIITGKGPDRKKYEEQIKRLKLRRVAFRTMWLASEDYPLLLGSADLGVSLHTSSSGLDLPMKVVDMFGCGLPVCAASFSCIEELVKVNSNGLLFSTSSELADELMMLFKGFPEECDTLKSLKDGALSTGSSSKWSTEWETNALPLVNQVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>S5SC28</accession>
    <name>S5SC28_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RA2</name>
        <name evidence="4" type="synonym">LOC123442810</name>
        <name evidence="3" type="ORF">Vrs4</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2013" name="Proc. Natl. Acad. Sci. U.S.A." volume="110" first="13198" last="13203">
            <title>Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley.</title>
            <authorList>
                <person name="Koppolu R."/>
                <person name="Anwar N."/>
                <person name="Sakuma S."/>
                <person name="Tagiri A."/>
                <person name="Lundqvist U."/>
                <person name="Pourkheirandish M."/>
                <person name="Rutten T."/>
                <person name="Seiler C."/>
                <person name="Himmelbach A."/>
                <person name="Ariyadasa R."/>
                <person name="Youssef H.M."/>
                <person name="Stein N."/>
                <person name="Sreenivasulu N."/>
                <person name="Komatsuda T."/>
                <person name="Schnurbusch T."/>
            </authorList>
            <dbReference type="PubMed" id="23878219"/>
            <dbReference type="DOI" id="10.1073/pnas.1221950110"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KC854546">
        <property type="protein sequence ID" value="AGS18321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KC854553">
        <property type="protein sequence ID" value="AGS18328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KC854554">
        <property type="protein sequence ID" value="AGS18329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="S5SC28"/>
    <dbReference type="SMR" id="S5SC28"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0194160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0194160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0194160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233930"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1926568at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="S5SC28">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF91">
        <property type="entry name" value="PROTEIN LATERAL ORGAN BOUNDARIES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="26"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGS18321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="26463" checksum="C0779A6A86BEFF40" modified="2013-10-16" version="1">MASPSSTGNSIVSVVVAAATTPGAGAPCAACKFLRRKCLPGCVFAPYFPPEEPQKFANVHKVFGASNVTKLLNELPPHQREDAVSSLAYEAEARVKDPVYGCVGAISVLQRQVHRLQKELDAAHTELLRYACGELGSIPTALPVVTAGVPSGRLSSAVMPCPGQLAGGMYSGGGGGGFRRLGLVDAIVPQPPLSAGCYYNMRSNNNAGGSVAADVAPVQIPYASMANWAVNAISTITTTSGSESIGMDHKEGGDSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287HKI1</accession>
    <name>A0A287HKI1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A287HKI1"/>
    <dbReference type="SMR" id="A0A287HKI1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0125280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0125280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0125280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0125280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0125280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0125280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287HKI1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF136">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0125280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="27786" checksum="EBCA3611F9241608" modified="2017-11-22" version="1">MLVGHLPFDDRNMVVLYQKIFKGDTQIPEWLSPSAQNLLRRILEPNPRKRISMAEIKIHEWFQKDYIPVAPYDDDDEDVRLGAILPIKQQISEAPGDKSTHQMNAFQLIGMASSLDLSVLFEEEGVSQRKIRFTSAQPPMDLFDKIEVSATQSGFHVQRAHSKLKITGNCNGPNNPTPFLVCAEVFELGPSLHVVELRKSHGDTAVYRQLCDRISSDLGIDKIFGMGSLFDDNLPSLDSRAATPLVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287HVV0</accession>
    <name>A0A287HVV0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Essential protein Yae1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123425841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965519.1">
        <property type="nucleotide sequence ID" value="XM_045109584.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965520.1">
        <property type="nucleotide sequence ID" value="XM_045109585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287HVV0"/>
    <dbReference type="SMR" id="A0A287HVV0"/>
    <dbReference type="STRING" id="112509.A0A287HVV0"/>
    <dbReference type="PaxDb" id="4513-MLOC_72857.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139320"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139320.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139320.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139320"/>
    </dbReference>
    <dbReference type="GeneID" id="123425841"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0114760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0114760.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0114760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0139320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0139320.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139320.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425841"/>
    <dbReference type="eggNOG" id="KOG4595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="YQLGYAQ"/>
    <dbReference type="OrthoDB" id="48036at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052436">
        <property type="entry name" value="LTO1_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532:SF2">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532">
        <property type="entry name" value="GEO13458P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="136" mass="15350" checksum="E6E04AEBF6AF7EF8" modified="2017-11-22" version="1">MDFLEPALALDETHYHEGYRNGYDDGLVSGKEEGRQVGLKNGFQVGEELGFYQGCLDVWMSVIRLDQDAFSARVRKYMEQLAALVSNYPLSDPEDEQLQGMMREIRLKFSVITGSLGAKLGYEGRPTSSEQDIEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MK73</accession>
    <name>A0A287MK73_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A287MK73"/>
    <dbReference type="SMR" id="A0A287MK73"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0319500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0319500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0319500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0266610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0266610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0266610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0319500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0319500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0319500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287MK73">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1095">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE RKS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0319500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="472" mass="52758" checksum="9880CE775E6D863E" modified="2017-11-22" version="1">MKPPDKFVRLWNKSYDDCKVECSKNCSCVAYAYLNLSTSDIDGDATRCLMWTGELIDMEKGGTIGNENLYLRLAALSSKGQKSIVMKVIPAISASVLLILLLACLLWLRKFKGKHDKEGRPKRLIIGGLCISDGPGEETHELPFISFKEIVAATNNFSMSNLLGQGGFGKVYKGLLHGNKEVAVKRLSRGSGQGVIEFRNEVVVIAKLQHKNLVRLFCYCVQGNEKLLIYEYLPNKSLDTFLFNSEKKSMLQWLTRFSIIKGVARGLLYLHQDSRLMIIHRDLKTSNILLDGDMNPKISDFGMARIFGGDEQQANTNRVVGTYGYMSPEYAMEGLFSVKSDVYSFGVLLLETVSGLRISSTQNIEEFPNLIIYAWSLWREELAMDLVDPSISESCSNEEVLCCIHVGLLCVQDDTDARPLMSTVVSNLESRSTPLSTPDKPLYFSQRNKAAKRADYGEDSVDKEALTVIEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGY5</accession>
    <name>A0A8I6WGY5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123412490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961374.1">
        <property type="nucleotide sequence ID" value="XM_045105439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062420"/>
    </dbReference>
    <dbReference type="GeneID" id="123412490"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412490"/>
    <dbReference type="OrthoDB" id="1914191at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34953">
        <property type="entry name" value="ALPHA/BETA HYDROLASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34953:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="217" mass="22829" checksum="F79EE63873AEE92F" modified="2022-05-25" version="1">MEKKLPLALALAQKHGAGEPAWARPWRWAKTAFFLVAMLASLLLVCAPPLLVVLLDLALPPMLLSAHLQAGADPPHRSFLPAVLDQARAFEFRSSLIDLPAVSAARALLILCAYTACGGGGGAYLWVVAASAAASVSYVLAKAAAVLPRGAAPQGKGDAGPEPMLLLSLSLAAAHVAVAYRTSCRERRRLLVYRIDVEAVRLKGGHQTPKGLKQCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIG0</accession>
    <name>A0A8I6WIG0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47169">
        <property type="entry name" value="OS01G0541250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47169:SF2">
        <property type="entry name" value="OS01G0541250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0034760.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="212" mass="24587" checksum="25B199DE3BAEDBDE" modified="2022-05-25" version="1">MNVCVRTVSRICNLVKEQVDLGQEVNVSSKKKGNVGRKRKELDLARTAMVPLNIRRTIRSLARCLGVPPSMLHDRFQLQEPKCITNTVKPFLKPANKIARLKFCISMMDKHWISTPYPSCKSMNNMVHIEVKWYDMTRVKNNYYVLPGEPEPERIVSNTNNIRKVMFLTAVANPWYNDEVEMRFDGKIGTWAFVVEKEAQQSSQAEIEERLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNX1</accession>
    <name>A0A8I6WNX1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF70">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28210" checksum="C9ED100DFF119512" modified="2022-05-25" version="1">MEECSKNICWEDLQRDALGIIFCKLSLQEILSVVPGVCKPWSEVVSGSYCWQDTDIQEWSQQSTTDQITSMVHMLLARSAGSCRRLSVSRLPNDSLFAFIADHAKSLETLEIPRSEISDSIVEDVAQRLSKVTFLDVSRCTKIGARALEAFGKNCKCLVRLRRVMHPMDLVGKVCHNDEAHAIACNMPKLCHLDLGYMFITTKAVFEIAFKCHDLKLLDMRCCWDVDDNLLREKFAGLKILGPRVYCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYS3</accession>
    <name>A0A8I6WYS3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="10">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123449651</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-asparagine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044982878.1">
        <property type="nucleotide sequence ID" value="XM_045126943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYS3"/>
    <dbReference type="SMR" id="A0A8I6WYS3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085120"/>
    </dbReference>
    <dbReference type="GeneID" id="123449651"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0070020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0070020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0070020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0085120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449651"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000055">
        <property type="entry name" value="Asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000024">
        <property type="entry name" value="Asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF47">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="4 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="341"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="591" mass="66316" checksum="C8274C369DB4916E" modified="2022-05-25" version="1">MCGILAVLGVGDVSLAKRSRIIELSRRLRHRGPDWSGIHSFEDCYLAHQRLAIVDPTSGDQPLYNEDKTVIVTVNGEIYNHEELKAKLKSHKFQTGSDCEVIAHLYEEYGEEFVDMLDGMFSFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDCERFISFPPGHLYSSKTGGLRRWYNPPWFSESIPSAPYDPLLIRESFEKAVLKRLMTDVPFGVLLSGGLDSSLVASVVSRHLAETKVARQWGNKLHTFCIGLKGSPDLKAAKEVADYLGTVHHELHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKATSAWGLEARVPFLDKNFINVAMDLDPECKMIRRDLGRIEKWVLRNAFDDDEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANAHVSDSMMTNASFVYPENTPTTKEAYYYRTVFEKFYPKNAARLTVPGGPSVACSTAKAVEWDAAWSKLIDPSGRAALGVHDAAYEEEKAPASVNPILENAFRSPAHGESTLVKTVVPTAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X159</accession>
    <name>A0A8I6X159_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123426950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044966810.1">
        <property type="nucleotide sequence ID" value="XM_045110875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X159"/>
    <dbReference type="SMR" id="A0A8I6X159"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027950"/>
    </dbReference>
    <dbReference type="GeneID" id="123426950"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426950"/>
    <dbReference type="OMA" id="HTRYMAQ"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000135">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF744">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027950.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="499" mass="53943" checksum="9939F3D938E514B9" modified="2022-05-25" version="1">MELPEDVRAADEESQPHFLIVTYPAQGHITPARHLARRLVRAAAGASGARVTICTPVSAFRKMFADATTAVGGGDEIREEGGVAYAAYSDGYDDGFDRAAGDYAHYMSQVRLEGARTLSGLLRRLGDRGHPVTCAVYTLLMPWVAGVAREYGVAVVAVFWIQPATALAAYYHYFRGSREAVMAAAASRDPNAEVCIPGLPPLRFRDLPSFLAVTSEDDSFACLIPEFRELIDALDGDREKPPTYVLANTFDAMEPDALASLRPHVQVVTVGPVLSFLHDGGHDDNSPNDLFDHNGQGYLQWLDTKSAKSVVYISFGSTSVMSKRQVTEIADAMSQSNRPFLWVLRKDNCKDTEEGDAIKGLLATATYDDDAGMVVEWCDQARVLSHPSVGCFVTHCGWNSTLESVACGVPIVAAPQYSDQGTNAWLVERALGVGVRAAARAEDGVLEAGELRRCMEIATSEAVASRAASWKEEARVAVADGGGSDRSLREFVRRTARGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1F8</accession>
    <name>A0A8I6X1F8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 19">Poly [ADP-ribose] polymerase</fullName>
            <ecNumber evidence="18">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC123448172</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="18"/>
    <dbReference type="RefSeq" id="XP_044980954.1">
        <property type="nucleotide sequence ID" value="XM_045125019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X1F8"/>
    <dbReference type="SMR" id="A0A8I6X1F8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0006690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0006690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0006690"/>
    </dbReference>
    <dbReference type="GeneID" id="123448172"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0004900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0004900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0004900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0006690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0006690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0006690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448172"/>
    <dbReference type="OrthoDB" id="2017365at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08001">
        <property type="entry name" value="WGR_PARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.130:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.630:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000004">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000005">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000051">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038650">
        <property type="entry name" value="PADR1_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008288">
        <property type="entry name" value="PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049296">
        <property type="entry name" value="PARP1-like_PADR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012982">
        <property type="entry name" value="PARP1-like_PADR1_Zn_ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF80">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21728">
        <property type="entry name" value="PADR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08063">
        <property type="entry name" value="Zn_ribbon_PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000489">
        <property type="entry name" value="NAD_ADPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01335">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52007">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="13 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 18 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 18 19" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="21">
        <location>
            <begin position="9"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="PARP-type" evidence="21">
        <location>
            <begin position="113"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="22">
        <location>
            <begin position="389"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="25">
        <location>
            <begin position="512"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="24">
        <location>
            <begin position="634"/>
            <end position="753"/>
        </location>
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        <location>
            <begin position="760"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000438"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00000459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50064"/>
        </source>
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        </source>
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            <dbReference type="PROSITE" id="PS51059"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0006690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="985" mass="110617" checksum="156B9511BEEF8BD6" modified="2022-05-25" version="1">MAAAPPKAWKAEYAKSGRSSCKSCKSPIAKDALRLGKMVQATQFDGFMPMWNHAKCIFNKKNQIKSVDDVEGIDALRWDDQEKIRNYAANSSTTSTATATATASSTAAIPDKCAIEVAQSARASCKRCSEKIAKGNVRVSSKLDGQGWYHVNCFLEMSPSASVEKFPGWETLSHDDRGAIIDVVKKGAASKQETTSKGSKRKIGDIDTHNSKAPNLNGSISEGAARSKGKLVVPCDPNSSSDDLHQKLKEQSDTLWKLKDELKKHVSTAELRDMLEVNEQDPSGPERDLLERCADGMLFGALGTCPVCNSCLYYYGGHYQCNGHVSEWSKCTYMTTEPVRIKKKWKIPDEIKNDYLTKWFKSQKVKKPERILPPMSPQKSVGQSPQQSFVGEALDKLRVCIVGQSKDVADEWKQKLKLAGAHFSPRVTKDINCVVSCGGLDNESAEVRKARRQKIPIVKEDYLGECIRKNRVLPFDLYKVATTLEESSKGSTVTVKVKGRSAVHEASGLQDTGHILEDGKSIYNTTLNISDMTQGVNSYYILQIIEEDDGSECYVFRKWGRVGSEKIGGKKLEEMSKTDAIHEFKRLFLEKTGNPWEAWEQKTNFQKQPGRFYPLDIDYGVKQAPKRKDISKMKSSLAPQVLELMMMLFNVETYRAAMMEFEINMAEMPLGKLSKENIQKGFEALTEIQNLLDDTGNQELALRESLIVAASNRFFTLIPSVHPHIIHDKDDLTMKAKMLEALQDIEIASKLVGFDGDNDESLDDKYKKLHCNITPLAHDSEDYKLVEKYLLNTHAPTHKDWSLELEEVYVLDRDGEGNKYSRYKNNLHNKMLLWHGSRLTNFIGILSQGLRIAPPEAPVTGYMFGKGLYFADLVSKSAQYCYVDRKNPTGLMLLSEVALGDMHELKKATPMDKPPRGKHSTKGLGKTVPLESEFVKWRDDVVVPCGKPVPASIRASELLYNEYIVYNTAQVKMQFLLKVKFRHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3V0</accession>
    <name>A0A8I6X3V0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dihydrodipicolinate reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3V0"/>
    <dbReference type="SMR" id="A0A8I6X3V0"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0131120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0131120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0108170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0108170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0108170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0131120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0131120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0131120"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:diaminopimelate biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Dihydrodipicolinate reductase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836:SF6">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE-LIKE PROTEIN CRR1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydrodipicolinate reductase N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydrodipicolinate reductase C-terminal" evidence="9">
        <location>
            <begin position="123"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0131120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="27080" checksum="0162678DE1D648BC" modified="2022-05-25" version="1">MGRAAIAAVSKARGMELAGAIDTQCIGMDAGELTGMDEALEIPVLNDLTMVFGSIAQTRATSVVVDFSEASSVYDNVKQAPAFGLSSVVYVPKIEMDTITELSPFCDKASMGCLVAPTLSIGSVLLQQAAIQASFHYNNVEIVESRPNPSDLPLPDAIQIANNISDLGQIYNRQDMDSDNPARGRVLGEDGVRVHNMVLPGLASSTSVVLSGPGEVYTLKHDVTDVQSLMPGLILAIRKVIRLKNLIYGLEKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7W3</accession>
    <name>A0A8I6X7W3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PORR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7W3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0268130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0268130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0268130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0268130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0268130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0268130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021099">
        <property type="entry name" value="PORR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045040">
        <property type="entry name" value="PORR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476">
        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476:SF2">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11955">
        <property type="entry name" value="PORR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PORR" evidence="2">
        <location>
            <begin position="63"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PORR" evidence="2">
        <location>
            <begin position="267"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0268130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="437" mass="48277" checksum="C1034B26CE20732D" modified="2022-05-25" version="1">MAPSSLSVLPAPPLTPLHPRRLRHPTTAPAPTTSQPLMLAVRCARAKVASSEPARPLPQPKLVRCPALDRQAARASRLRFAHKLLTLLLSKPCGFLPLRVLLRCRRFLGLPRRRQLVPFVLCYPTLFRLFQAPISRPFSPSLSTLAVALTPVAHALVADLAALCGDELALGLADKVHRLLLMTPRRRLLVSKLAHIAPDLGLAMDFRATLCPPPPGHHQLRKHLPRPRAPPGRLATAAPFTAPSAVPPRRPARPPHRPAAEPLEEGASTETAERRACVVVREVLAMTVEKRTLVDHLTHFRMNFGLPNRLRALLVRHPELFYVSIKGVWHSAFLVKAFDNDGRLLVEDGMLVGRDRLEELVREGKKIRQARKKGIFPVDGGDTDEDDEEDDNVVEGTSAVDGEFGDLFEQDSVAGEDWNEKDAEEGVVDSGSEHDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X984</accession>
    <name>A0A8I6X984_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123424565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964132.1">
        <property type="nucleotide sequence ID" value="XM_045108197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X984"/>
    <dbReference type="SMR" id="A0A8I6X984"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0107060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0107060"/>
    </dbReference>
    <dbReference type="GeneID" id="123424565"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0087740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0087740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0087740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0107060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0107060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0107060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424565"/>
    <dbReference type="OrthoDB" id="6119954at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010179">
        <property type="term" value="F:IAA-Ala conjugate hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009850">
        <property type="term" value="P:auxin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08017">
        <property type="entry name" value="M20_IAA_Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044757">
        <property type="entry name" value="ILR1-like_Hyd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF149">
        <property type="entry name" value="IAA-AMINO ACID HYDROLASE ILR1-LIKE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035231311" description="Peptidase M20 dimerisation domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="217"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="429" mass="46184" checksum="AD9C13C6FB0A3A4D" modified="2022-05-25" version="1">MAWFLTHLVALFLLFSSHLVTPSSAATTRLGADLLGAARAPPFESWLRGLRRRIHQRPELAFQEHRTSELVRAELDALGIPYVWPVAHTGVVATISGGGGGPGPVVALRADMDALPLQELVEWEYKSLENGKMHACGHDAHVTMLLGAAKLLESRKENLKGTIKLVFQPAEEGYAGAYYMLEEGVLDDVSAIFGLHVFPHFPVGVVASRPGPFLAAAARFTATITGKGGHAGNPHDAVDPVIAASSAILSLQQLVARETDPLEAAVVSVTQLRGGDAYNVIPESASFGGTFRSMTDEGLSYLLKRVKEVIEAQAVVHRCVAIVDFMEEKLKHYPATVNDEGMYAHSKEVAEAMLGEANVKVAPQSMGGEDFAFYAQRAAGAFFFIGVGNETNMDRVRPVHSPHFVLDEDVLPIGAAFHAAVAIEYLNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9R4</accession>
    <name>A0A8I6X9R4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9R4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0071990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0071990.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0071990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0087380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="13547" checksum="649D5AD927B33BEA" modified="2022-05-25" version="1">MAGRGVQLNRGNIAGLWSDDLGDNFLLKDKYLELFEICINKECTVDKMESLNHLSAFRRRLSSEMLQKWNDMRKYVLGKTDKVKNDEIYWKLDNSREYSTKSMYRWLEKKHCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XA14</accession>
    <name>A0A8I6XA14_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123450252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hordeum</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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            <begin position="109"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0453280.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">cv. Morex</strain>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045108303.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424652"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
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        <property type="entry name" value="ZN346_ZMAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144:SF6">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="9">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="332"/>
            <end position="382"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="41271" checksum="F2990081FECCAA9B" modified="2022-05-25" version="1">MDYAAHAAATAQPAADPGATYPPGYPYAYAYPAYHYTDPAAVAPSAPGSAASSSSYYSNPAAQAITGPQYNPAAQASTGAQYNPAAQASTGAQYNPYTGQYHYFGAPATDGGSGLADYYFTAGEASQAPAASGAQTAAAAGREAVKQFGFDPQRYAQVAAAKAPNGVGPTAAATVMHHAQWNAHFGHPLPTNVRRSFRKKTPKFVQPLTCEVCKIQCDTMDVLLIHKTGQKHKKNLQKLQDAITPQPAKPPNSGVAASTAPPTAPPAAAATAADTVVPAVQPKKNKSSNASPADLEVKKRRVIEAGAAHGEMKICTACNVVVNSQKVYEFHLAGHKHKANVLKLEKKQLQQQQQQVQLQQQQQQQQQQQVQLQQQQQQQQQQVQLQHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XD85</accession>
    <name>A0A8I6XD85_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123429558</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969518.1">
        <property type="nucleotide sequence ID" value="XM_045113583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XD85"/>
    <dbReference type="SMR" id="A0A8I6XD85"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0117830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0117830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0117830"/>
    </dbReference>
    <dbReference type="GeneID" id="123429558"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0096850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0096850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0096850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0117830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0117830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0117830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF29">
        <property type="entry name" value="OS07G0656000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="12">
        <location>
            <begin position="79"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="11">
        <location>
            <begin position="131"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0117830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47543" checksum="4D95530281CE14F7" modified="2022-05-25" version="1">MEMASARRRARQLSGDHAVLWSACVLLSAASLLLAATLSSGFGLAGEVSVVVRASAGAVVVTTDDGRPYCGDVGRDAMLTDGEWVREEAGAAPLYDPRECPFVDVGFRCRENGRPDDGYARWRWRPRHCELPRFDAKKLLEALRGRRLVFVGDSIGRNQWESMLCMLSSAVADAGASVREEHGSPITKHKGFLSFRFLHHNLTVEHYRSPYLVRRGGRPRPRRAPRHVRSTLQLGAMDSRAHLWKGADVLVFNSGHWWNQDRLQQLHCYFQEGKRLRLDMSVEEAYQRAMDTVHEWVQKEVDGSKTLAVFRTYSPAHNRGTNGGSCAMETLPELNMTGISLGRWPGMLLPAFGGSESSAGARDLRVMNVTLMTAQRRDGHPAVYNVEPSARMPVGQRADCSHWCLPGVPDAWNELLYAMILRRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFZ9</accession>
    <name>A0A8I6XFZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Growth-regulating factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123430629</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription activator.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The QLQ domain and WRC domain may be involved in protein-protein interaction and DNA-binding, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GRF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970419.1">
        <property type="nucleotide sequence ID" value="XM_045114484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186750"/>
    </dbReference>
    <dbReference type="GeneID" id="123430629"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0154640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0154640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0154640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0186750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430629"/>
    <dbReference type="OrthoDB" id="1927209at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031137">
        <property type="entry name" value="GRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31602:SF85">
        <property type="entry name" value="GROWTH-REGULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31602">
        <property type="entry name" value="GROWTH-REGULATING FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08880">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08879">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51667">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="QLQ" evidence="8">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="WRC" evidence="9">
        <location>
            <begin position="136"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Bipartite nuclear localization signal" evidence="5">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Bipartite nuclear localization signal" evidence="5">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24566" checksum="FC9FBC58C8D4EB31" modified="2022-05-25" version="1">MTERRQEEHSPPSKLPRLSGADDDADADANDGVGTVTMAAPLPLALGLGLGVGGCCSDSRRSDAEATRPSALTFMQRQELEHQVLIYRYFAANAPVPVHLVLPIWKSLAASSSAPQRFPSLAGLGSMCYDQRSSMEPEPDRCRRTDGKKWRCSHGVVPGHKYCERHVHRGRGRSRKPVEAAAATSATPIRAMHAADGQGATGAHAHAPPQRLSFSSPAGVYLAHGTARAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIX5</accession>
    <name>A0A8I6XIX5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123398747</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949139.1">
        <property type="nucleotide sequence ID" value="XM_045093204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIX5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440780"/>
    </dbReference>
    <dbReference type="GeneID" id="123398747"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0364890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0364890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0364890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0440780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398747"/>
    <dbReference type="OMA" id="IMFPIFQ"/>
    <dbReference type="OrthoDB" id="692040at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36479:SF11">
        <property type="entry name" value="NPH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36479">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035210086" description="Ubiquitin-like protease family profile domain-containing protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="164" mass="19077" checksum="043933FCEA305F9C" modified="2022-05-25" version="1">MLSPLLLLRIMFPIFQSLAPNNLVDKCGHHHTTCTDLKHQRFEVLESLRSGDDTILTSHVDFFINILKETWNRHHGSSNVQSSHFPIEYVLTTKQGNGYDCGYHMLEYLAKWQGIKVPSIRKACVAELHKILTWNWVTNGDFNKRPSPWDFIEEAVKPARKKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJJ6</accession>
    <name>A0A8I6XJJ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123442578</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974598.1">
        <property type="nucleotide sequence ID" value="XM_045118663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJJ6"/>
    <dbReference type="SMR" id="A0A8I6XJJ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0226410"/>
    </dbReference>
    <dbReference type="GeneID" id="123442578"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0187890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0187890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0187890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0226410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0226410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442578"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="39129" checksum="C8677F4FFACA9846" modified="2022-05-25" version="1">MIESYGSLAPKRYMYSEVMKITSSHSDLLGKGGYGVVLKGRLHDGRLVAMKFLHDCKGNGDEFVNEVMSIGRTSHVNIVSLFGFCLEGSKRALIYEYMSNSSLDKYIYSESPKAILGWERLYAIAIGIDRGLEYLHHSCNTRIVHFDIKPQNILLDKDFILKIADFGLAKLCHTKESKLSMTGARGTIGFIAPEVHYPTFGVVSTKSDVYSYVMMLLEMVGGRRNVKSIVAKSSEKYFPDWIYDHFAEDDGLQACEVTIEIEEIARKMIIIGLWCIQVLPMYRPTITQVLEMFERNLDDLDMPPKQNFCELLESSAQNMDVQSSSSTIPEEISLVNSKILQQSRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJX8</accession>
    <name>A0A8I6XJX8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0508570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0508570.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0508570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0508570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0508570.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0508570"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0508570.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28965" checksum="B852E246E5A9A232" modified="2022-05-25" version="1">MLGHPSRQVVRLTRCIDVLGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEIEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKK3</accession>
    <name>A0A8I6XKK3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKK3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0238500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0238500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0238500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0238500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0238500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0238500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0238500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="16185" checksum="A30D11BF0C7B1B28" modified="2022-05-25" version="1">MVDKACSTKASSVTFIERLFTELAEVGEKKEEVFNLETSGGQDALKESVDKHGGCPGDKTVKDYSTQCGADCDKACMDPTCTDHCYVDCPYMAARFGYIMATPTNKKAIEQDIDCFKHCAKEKDSSDNKCRDTCKLISSTPTPAPAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLN6</accession>
    <name>A0A8I6XLN6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449093</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982114.1">
        <property type="nucleotide sequence ID" value="XM_045126179.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6XLN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0390240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0390240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0390240"/>
    </dbReference>
    <dbReference type="GeneID" id="123449093"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0390240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0390240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0390240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000116">
        <property type="entry name" value="p-loop containing nucleoside triphosphate hydrolase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF1">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="811"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0390240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1289" mass="141920" checksum="2BF195E48BC45A43" modified="2022-05-25" version="1">MGGSADPEDPAPMPTPTPSPTPSPAKPSPSSSDAKRLRRCVQSRLPFGSGRSGGGGGGAGVAPPAPAAEEAAGKEAAEEPEKAKRKARPRRSAAARKPSSNKETTGLDPGSKDEAILVDESPQKKQRKGRNQDAARKAPNRKRCKVLESPDGHRSCQQLHSNQTEAILPEGSSVSIDIDLNNVPSEATQANDNNVLDNEDKSQVIVDLRSEAKIAAQEIRMLSSGKKLHPFFASRKVNKGAEQDAFNNEDTNSLGAIERDPPFWPVHVVYQLETDIPIHWSKWLIAERSFLDTSASDTLENPVSFFEDIVKPLTIESNSKKMCLDQLAEQNVANHTAFGMDFPSFPKEQCEGNLSSLDVIHLDEESCPHDSLSYNKHPERILQGRAEVDQKGCLPDYYLWTDKYRPETAAQVCGNIEHVKFLSEWLKGWDERGHKNKQTGVANESINASSCQDESDTDCSEDASDYENVLLITGPVGCGKSAAVFACAREHGFNVIEVNTSDMRNGAYVKQKFEEATKSHGLEKWSQEEVTTPPRIDSLDPASETPDRTEYKCSVSCSTRKTCNDDEHMLPVKCYSSSKLSDEAPKQVINKTLILFEDVDTVFDEDRGFISTILKIAETTRWPIILTSNKKDPALPHLLDQLVLDFKYPSSGELLSHLGMICKSEGVEVTAPQLKYFINACLGDIRRTTLLLQFWYQGKQEYTERSNKCLSGPFSLDLDAVHSTVPRMLPWDFPCKLSETVCLEIEKTIHLAEEKKRQMELSEFEALELQITAPLSKGRGAVKTTKIKKSKLKHGHSTECNAISPCKNDLDDFDDAPETSLPSDQQKTRKKHGVVLLSESDDDQTDAYIAKDARFTLPEGDLFPQPPELPHIHGQGISNQFCFPSESRETFEIANSFQNQFESNLVGSISQICDTFMSQGVSCVPESSLALGGVSASVSSDDLLSSMVFNGLSTFNNDGVCTKPITSLEDSNHSRNLMAGSQKCMEDVVGETCEVYAESFGRNEPESCSTTGYQLMDECSRADSTWLLSGKKTNDCCKVEHVQDTWNRLRRCCPVLPSETNHNRTASGALKLASGVSDLISESDLMVARCHPLTNDILDPSSTPSAEPDDLSWYDKQLEMGSVYAQHALCVFSRDLQDKEDGFIDLSQELLFASTTVTSLGKLISSGISSDDGYGNISHMKNPTSCISKGREQLVHLCGALLPVVPSKLSLSLRGPAFVDYLSSTCQISQLENLRLTDSKAANKQRRCRQSRHYLSSAALSMSPEDIELLAQSNCFGDRREKVIDQAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLW8</accession>
    <name>A0A8I6XLW8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLW8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0370010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0370010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0370010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0446760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035274982" evidence="1">
        <location>
            <begin position="23"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="116" mass="12402" checksum="06FE7242FE393E79" modified="2022-05-25" version="1">MRGSSLAQATTIFFIGCLVVSAQCGLGANQRVVIRDDRAANTSTDTTAVNSTSVGDSMITVKFCIQFLRCHLSDGYRGTCYCCQKDEPQCFTTKSECQDNCPDCNPNCPPPSGSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XND9</accession>
    <name>A0A8I6XND9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XND9"/>
    <dbReference type="SMR" id="A0A8I6XND9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0185430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0153540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0153540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0153540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0185430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0185430"/>
    </dbReference>
    <dbReference type="OMA" id="ENDELAC"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF50">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="6">
        <location>
            <begin position="138"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="214" mass="23832" checksum="77F42A05F5927277" modified="2022-05-25" version="1">MLTLFLEPGNHQPALRTRLPYLDPEFLTTGELTPLSDVYSLGVIILFLLIGLPPLSIGKKVSEALENDHLNTLIDKSAGNWPYVQAKQLAVVALSCVEMTREKRPDLLTKVWSVIEPLIRKPPVASWPCVQSAVRGSCVPDNLICPIRMDIMKDPQVASDGFTYEAEAIRRWFDGGNNRSPMTNLLLANRALVPNRALVSSIQEYLEQQRQPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPC6</accession>
    <name>A0A8I6XPC6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Splicing factor U2af large subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">U2 auxiliary factor 65 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">U2 small nuclear ribonucleoprotein auxiliary factor large subunit (U2 snRNP auxiliary factor large subunit)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123448521</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Necessary for the splicing of pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPC6"/>
    <dbReference type="SMR" id="A0A8I6XPC6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369460.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369460.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0307580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0307580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0307580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0369460.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369460.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008187">
        <property type="term" value="F:poly-pyrimidine tract binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12230">
        <property type="entry name" value="RRM1_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12231">
        <property type="entry name" value="RRM2_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000057">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000111">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000225">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006529">
        <property type="entry name" value="U2AF_lg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01642">
        <property type="entry name" value="U2AF_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="230"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="350"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369460.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="566" mass="63167" checksum="46B2578EC42A3625" modified="2022-05-25" version="1">MAEYVERYKGNGEPASVTGVSPPTKPTSFSDQPDGRSQQEVQPHEGRSSKSRERDRGREKDKDKERDREHGRDRERGRDKDRERNRGDRDREHDRHHREHRERSEKREHRGHSDDHDRHRSRDHDVERRDRDRDGHRRHRSRSRSKGRERRSRSRSRSRSKSKRVSGFDQGPSQAIPMVTPGTTPGQLPAVAPLITGMLPNMFNFTAPTQFNPLVMQPQAMTQQATRHARRVYVGGLPPTANEQTVAIYFNQVMAAIGGNTAGPGDAVLNVYINHDKKFAFVEMRSVEEASNAMALDGIMFEGAPVKVRRPTDYNPSLAAALGPSQPNPNLNLGAVGLTPGSAGGLEGPDRIFVGGLPYYFTEAQVRELLESFGPLRGFDLVKDRETGNSKGYAFCVYQDLNVTDIACAALNGIKMGDKTLTVRRANQGTSQPRPEQETILLHAQQQVQMQKLVLQVGGALPTKVVCLTQVISADELRDDEEYEDILEDMREEGRKYGNLVKAVIPRPDPSGAAAVPGVGKVFLEYADIDGSTKAKVGMHGRKFGGNQVVAVFYPENKFTDGDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPI5</accession>
    <name>A0A8I6XPI5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6XPI5"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS04G0448401 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="151" mass="16552" checksum="05B21EBEEFA5B6D8" modified="2022-05-25" version="1">MINVDAGLFTHDRSMGVGVVMREHSGSCIVCCGIGVSDVILPELAEAIAIQRGLHFALCEGIQEVLLGSGCLTVVQRINSLGQDRSICGRVIQDIKQLRALFTSCSIFHVRREQNVVAHLLARSCNQLECRVWRGVSSKCIRDAICMEIMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQ34</accession>
    <name>A0A8I6XQ34_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Btz domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CASC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQ34"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285350.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018545">
        <property type="entry name" value="Btz_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044796">
        <property type="entry name" value="MLN51_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46837:SF7">
        <property type="entry name" value="BTZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46837">
        <property type="entry name" value="PROTEIN MLN51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09405">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01044">
        <property type="entry name" value="Btz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Btz" evidence="14">
        <location>
            <begin position="99"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="498"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="794" mass="87665" checksum="A25DB0370C7EDD32" modified="2022-05-25" version="1">MAATAAGDEEEELSDVNDSPLPALRRREAASDDDDEGDGGGGGGGSGSPPPSRVAGSDSDPDSEELGAAEVYDEDEGSEECEEARKEFDAGSNGGGEAKEVAPDEVAAAPEDGEAQAETGAELEEEDKESKGSEPHAVPRTGAFYMHDDRFQNKENRSPGRQRDFFGGQKLWNSEDDSVWLHDRFDEINTHYVQHDSTRRPRSPFKAWAGGRTHDVNHDRFDEINTHDVQHNSTRRPRSPFRAWAGGRTHDVNHDRFDEINTRNVQHNSTRRPRSPFRALAGGRTHDVNHDRFDEINTHYVQHDSTRRPGSPFRAWAGGKTYDVNHGYLQGTKSAFYYHDYGADYKYGSTNNYNRFPEETNTSYYSANNYRSVPRKSHLYYDEKNFYNARAESRICYVNAKGYNNAPNVNRGKPSRPYQPHWKNTFETSSVQNNRTYSRSQNEEGCSDTGVGKNSHRTLSLQNEQEFPSKQEFPFIERRKSRPDILSKLFSSSIRMAHSSLKPQSRPSFGVKAFAPSGEHGNTAGSLSMIKGMPDLGSRSSLSTSNSQYSKSRDQGSGLNIGGPTKNNLSSTSQATRSYTEFSNAVYQQRSVQQPLLLTPSESTQIFHQNIASTSKIQSHPQNTLTNPPPGSNKSLAPSVIIAQNDKAEAVSGSCPCGDHALDVTGAKGLTLGTPAVLSVMKFSGQHPRGPDIPCTGMDVPGLLVHQSSDISELSQITWLQTLSGATGVLGETYDPSYIGSHYPQPSAFPRNRCVTEVPVLLNSPEIPEVVGHELGQRKNKLLRYSEMNFASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQH5</accession>
    <name>A0A8I6XQH5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0481660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36712">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36712:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="68" mass="7800" checksum="E6A8031A423EF919" modified="2022-05-25" version="1">MDILYLCKDVLKIQKFRRLASYAGLYSFTTLVTYAYTSNTKRAGISRADQYYAAYPSRTELLTDATKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQN1</accession>
    <name>A0A8I6XQN1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="5">HMG-CoA synthase</shortName>
            <ecNumber evidence="5">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQN1"/>
    <dbReference type="SMR" id="A0A8I6XQN1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413410"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00102"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF6">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="5" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="7">
        <location>
            <begin position="108"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="8">
        <location>
            <begin position="280"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="566" mass="63562" checksum="BF2561C6E2172A50" modified="2022-05-25" version="1">MRIGRRPRARPDAPAGRYYRGHPAPQLPRPPTARYHPPSPRKKKEKRPAASSVYIRRGWSGRVPHPSPRSAHQSRPSPTWRSFSVFFPHRSSLESAEERRKMAAERVRDVGILAMDIYFPPNCVHQEELETHDGVSKGKYTIGLGQDSMAFCTEVEDVISMSLTVVKSLLKNYNIDPKCIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPISFESKFRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYKTFCKRYEKIEGKPFSIVDADSFVFHSPYNKLVQKSFARLYYNDFLRNCSIVDKDSREKLEPYSGLSSEESYQSRDLEKVCQQVAKPLYDTKVQPTTLIPKQLGNMYTASLYAAFASVVHNKHDALVGQRIVMFSYGSGMTSTMFSLKINEVEHPFSLSNIASILDISNKLESRHVVPPKKFVEALKLMEHRYGAKDFITSQDTRLLVPGTYYLTHVDSMYRRFYAVKGDAAATPITNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRL9</accession>
    <name>A0A8I6XRL9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRL9"/>
    <dbReference type="SMR" id="A0A8I6XRL9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0306320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0306320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0306320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0255850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0255850.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0255850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0306320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0306320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0306320"/>
    </dbReference>
    <dbReference type="OMA" id="AMTINIK"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF93">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035277867" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0306320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27151" checksum="5BCBDDA9D15382D7" modified="2022-05-25" version="1">MTAMTINIKFLLWLLLLFSASFCHGSELDIQCLKSVQKSVADPNGILKSSWKLNSSGVSGYICESTGVECWSHDQNRVLSLRLSSLGLQGPFPKGLDDCTSTIALDLSGNRFSGPIPSRIAHDAPHLSYLDLSNNGFSGEIPDSIADMVYHNVLNLEHNRLDGPIPERFNALGRLTTLSVADNLLSGSIPALLRKFPASHFAGNQGLCGAPLERPSGGRRVRGGLLLLLVLYFPRWLGLSKRLRASGLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUW2</accession>
    <name>A0A8I6XUW2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Legume lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUW2"/>
    <dbReference type="SMR" id="A0A8I6XUW2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0325350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0325350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0325350"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0325430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0325430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0325430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0325350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0325350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0325350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0325430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0325430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0325430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050258">
        <property type="entry name" value="Leguminous_Lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401:SF57">
        <property type="entry name" value="OS08G0334300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043264845" description="Legume lectin domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Legume lectin" evidence="5">
        <location>
            <begin position="27"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0325430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="386" mass="42800" checksum="3093BED2824655CA" modified="2022-05-25" version="1">MAHARSILIPTYFLFLAAYTTAHAAALSFDYNFTVPGDREHLKFINASYAGRARIILTDDSSNLIGRVVHRKPVRLWDDRTGRTASFTTSFRFAIVQDSNMTDNLSRGDGMAFFLGPFPATTPPPGSDGGLLGLFSNPNSTGAADSRRPPHTLAVEFDTRWNDGWDPTNEDGPDYIGIDVNGIRSNRTRSLPPLSLHGIMWASVTYDAESKVMKVALRKTELASESSTTYEFNATMDLRDDAGLVQDAAVGFSAATGVLCESHQLLAWSFHSTDPSHTETKMWVIFLAVTAVLLAGLLVALLSLIYTTFIKRPTRCLDGNCLQVARKFSYSELVAATDNFSRDRKISKGSFGDVYRELLPMPDLREVAVKEMRQPTLKRNRKNYVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVB4</accession>
    <name>A0A8I6XVB4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123395052</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVB4"/>
    <dbReference type="SMR" id="A0A8I6XVB4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0532730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0443090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0443090.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0443090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0532730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0532730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000196">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g14850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000243">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000738">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000285">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF364">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01535">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
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            <begin position="47"/>
            <end position="76"/>
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            <begin position="54"/>
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        </location>
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        <source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="745" mass="80578" checksum="9FEAEF0A3D5341A3" modified="2022-05-25" version="1">MCILLLGQLRARLGPCKQLGPVETLDRSLSISSSLVPVRRQALCHGSGHGRAMAAPHATLPPATASPSPRRGRLRRETAEVVRDCKSLDGLMKAGRVADALDLFDRMPRKNVVAWTSAVSGLTRNGRPEAAVEMFADMVESGVALNDFACNAALAACAAAGPGALRTGEQVHSLAVRAGFVGDAWVGSCLVELYARCGSTRAAEAVLARMESPDVVAYTSLVSAFCRSGEFGLAVEALGQMMGRGVIPNEHTVTSILAAACCPPVLGEQIHGYIIKATSSPSQSVYTSSTLVDFYSRNGEFDMAKTVFDNLQCKNVVAWCTMMQLHIRDGRPEDALQLFDEMMSEGVVSPNEFAFSIALGACEFIALGSQLHSLAIKRGLASDLRVSNALLSMYGRIGLVQQLEAMFRGIEDPDIVSWTAAISAYFQNGHGEKAIALLSRMHSQGLTPNDYAFSSVLSSCADLALLDQGRQFHCLALKLGCDVKICTGNALINMYSKCGQIVPARLAFDIMDHCDVTSWNSLIHGYAQHGEVDMALKAFSEMCSDGGEPNESTLLGVLAACNHAGLVDEGVAFFRSAMAGLYGTFLTPSHYACVVDMLGRSGRFDDALCLIEEMPFEPGVLVWKTLLASCRLHGNLETGRLAAKKLVELSDQDSASYVLMSGIHAMHGEWRDADMVRRRMDEAGVRKEAGRSWVEVRNEVHTFVAQDVSHPESPSIYRMLWELSDAMRDTAYDEDVELLDVHMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="744" mass="83012" checksum="90345A50C849173D" modified="2022-05-25" version="1">MAVSNGARCGGITETFGTVEERLLRLEVVVLVSALVLASLVLYGSTQRRSSDRVLRGVMWIAYSLSYVVVTYAVGIIQEGPFHGQTFVLWASALLLIQASAYAAPVHSRRDVDQRKKLLLQHALQTALVLWLILKAIGANTSYRAAIWAFWSLSVVKTAAKIVEMVRSSLPDPSVKVVAEYMDVEDFLAADQLPHDPATMKGYKYLFHGEEAIQRQLSHQPRSSQDEMLMTTASSSALTRTTSSILTATISSGPAPDKVKGVVTIDQVYRWIDRQGYSDAEKDMAKDFCLAFSLFKLLKRRFYGYIPAEAGSPKSLDLVLTGLIHPAVTGPDAAFRVVEAELAFLYDFLYTRNILPVGVKTYIFIAVVMLSMTMWTAFVGTLGPGYSRPHVGVKDLDRSVTVVLVVITAGLEVCQALAAFSNNWRYVMTVYRCVRDEQPWTKQRHGHLWKQNITPPETTYWEQKIGQYVLLHWYDHRPWNVKSLITLYLVEPRRQGQKKGSRKALPQQVKRAVLASLKASHGELTNGIATVERHGLSQQLTWACRLPKVTDQILVWHVVTTRFKWISSGRGDGDGVDQHRLVARKLSNYCAYLVAFVPEMLPDPSYNAEQIFDTAVRQARTHLDGCRTKADILDRLEKIQSKEEDYLEGGSLYERAGSSSIIEKAALLGGQLMSSVPDEGRRWKVLAEFWAEFILFLAPSDNVDIHAEMLGAGGEFMTQLWALLSHAGILERTVAATPSAPART</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XY79</accession>
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        <name evidence="2" type="primary">LOC123402513</name>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045096433.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123402513"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0547360.1">
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="80"/>
        </location>
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        <location>
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            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="171" mass="16856" checksum="266D95C89D7C5049" modified="2022-05-25" version="1">MASLALRPIMPATATATAASTTTTLNPARRGSSTLLLLRRRQPVTCMAESSGGGNSTVELAAGAAGLASCATVAWSLYTLKATGCGLPPGPGGSLGAAEGVSYLVVAGIVGWSLTTKVRTGSGLPAGPYGLLGAAEGVAYLTVVAIAAVFGLQFFQQGSIPGPLPSEQCFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYD3</accession>
    <name>A0A8I6XYD3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYD3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0447810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0447810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0447810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0447810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0447810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0447810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0447810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26552" checksum="B7316C6B3F692E67" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFLDYPGAEKPIHWSYGLGVTVDMGVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGFYFDSSMEDPLLQTTVEMLENRDNDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRRPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZ84</accession>
    <name>A0A8I6XZ84_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC123404230</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044954079.1">
        <property type="nucleotide sequence ID" value="XM_045098144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZ84"/>
    <dbReference type="SMR" id="A0A8I6XZ84"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561620"/>
    </dbReference>
    <dbReference type="GeneID" id="123404230"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0466390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0466390.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0466390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0561620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404230"/>
    <dbReference type="OrthoDB" id="647974at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000403">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF155">
        <property type="entry name" value="LOW QUALITY PROTEIN: RECEPTOR-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035292307" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="37"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="667"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="751"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="780"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1028" mass="112561" checksum="39C7138484D8C36E" modified="2022-05-25" version="1">MRTLHFSDKTHSKIYHIPSLGLALVLLICLASPSSSCTEQEKGSLLQFLAGLSQDGGLSATWRNGTDCCKWEGITCWQHGTVTAVLLPSKGLQGHISRSLGLLTGLQYVDLSHNSLSGGLPPELMSSRSITVLDVSFNQLNGTLQELPSPTPARPLQVLNISSNSFTGQFPSTTWKAMVNLMALNASNNSFTGQMPTQLCSTSPSLTVLQLCFNEFSGRIPPSLGNCSRLRELGAGYNKLSGAIPDELFNATSLEYLSLGCNNFTGKIPVSIGKLKKLEKLHLANNNMSGELPSALSNCTNLLIIDLKSNHFSGELTKVNFSNLRNLKTLDLWSNNFNSTVPESIYSCSNLIALRLSKNHLHGQLSSRIANLKHLSFLSLGKNNFTNITNALQILKSSKNLTTLLIGHNFRGEILSQDETIDGFGNLQVLDIQGCKFSGRIPVWISRAANLQMLLLCGNRLTGPIPGWISSLSHLFYMDVSSNSLTGEIPLTLTEMPVLKSIDNATQLDSTVFELPVYNGPALQYRVVTSFPAVLNLSNNNLTGVIPAQIGQLKVLAVLDFSFNKLSGQIPQSVCNLINLQVLDLSSNNLTGAIPASLNRLNYLSAFNISNNDLEGPIPSGGQFNTFQNSSFSGNPKLCVSVLTHKCDSALIPPSSIKETTKPRDKMAVFVIAFGVFFGGIALLLLLGRLIVFIRINGVRIRNRRENNGDVEETSFYSSSEQTLVVMRMPQGKTEGTKLKFADILEATNNFDKENIIGCGGYGLVYKAELPDGSKLAIKKLNGEMCLMKREFSAEVDALSMAQHENLVPLWGYCVQGNSRFLVYSYMENGSLDDWLHNRYDDAISFLDWPTRLKIAQGASMGLSYIHDICKPQIVHRDIKSSNILLDKEFKAYVADFGLARLILPDKTHVTTELVGTMGYIPPEYGQAWVATLRGDMYSFGVVLLELLTGRRPVTVLSTSKELVPWVLEMRSEGKQTEVLDPTLGGRGYEEQMLEVLETACKCVDNNQFRRPAITEVVSSLASIEAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZN7</accession>
    <name>A0A8I6XZN7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZN7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0489350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="19170" checksum="67C1CCEEB5DB4BB2" modified="2022-05-25" version="1">MCLRDQHLWRVRCPMICFYAVEWHLPHRVSKQFGVQQRTPPDYVETSVKLHKINRQSNKTVISWEEHHITWVDMWNAQRQHRVENDETPDTDEAAYLRHLEWMRTEYRVIHKSAWTRSVCLDLLPSEAADAAFNNSIRETIGAHLGYGPLHDRVVRPSFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3I1</accession>
    <name>A0A8I6Y3I1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3I1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0274160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0274160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0274160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0329270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="82" mass="9523" checksum="3A5975C0D6255391" modified="2022-05-25" version="1">MVAMLKNGLCNSLFSTTIWSRKCAVYGFFITSIESSICTVCTGSSCNFIVHKHIIHNNVIVTIRVSWYHKRKHRQSKSFWIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4L3</accession>
    <name>A0A8I6Y4L3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123447517</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980060.1">
        <property type="nucleotide sequence ID" value="XM_045124125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4L3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337720"/>
    </dbReference>
    <dbReference type="GeneID" id="123447517"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0281670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0281670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0281670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0337720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447517"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15556">
        <property type="entry name" value="PHD_MMD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057765">
        <property type="entry name" value="MS1-like_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059080">
        <property type="entry name" value="WHD_PTC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058054">
        <property type="entry name" value="Znf_MS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46201:SF9">
        <property type="entry name" value="PHD FINGER PROTEIN MALE MEIOCYTE DEATH 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46201">
        <property type="entry name" value="PHD FINGER PROTEIN MALE MEIOCYTE DEATH 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25565">
        <property type="entry name" value="Ubiquitin_At1g33420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25874">
        <property type="entry name" value="WHD_plant_repro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="7">
        <location>
            <begin position="613"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="677" mass="74653" checksum="3B18055DBEE2AED2" modified="2022-05-25" version="1">MPSIRALMRLAAPLPPRADAAAEESPPSSSSSASKPAGAGRRSVPPPPQQQQPQGRAYPLRDFPGSEAAALCGAFRDNARWLLARWGPVGASPGPAARRAFLSDDRTGALVPVVAVEVLAASSPAPLCDLCRCAGWSHHWVSKRSYHFIIPADADWDRHFGTDALLGRNDHLLHGLIHCNGFGHLVALRGRDGGSAFLSGHDIMDIWDRLCSALRVRAVSLVDFSRKQSMDLRLLLGVANGETWFTRWGYCLARRCFNMSTSTYATALESLASLHVDHLRSRHVRRVVTIYRRLSNKPLMTVREFLRCLLDWKRHEAPLTPPPVKPCSRLPFLLPKPCTMKRSPPCKRFEDVVERLNCRFSKKRLVNAAEVVVEKLLEHGNDAEMTRQAVRDAARVEIGDTGLLDFVIKSVGDTVVGNHVVRRLHNTATRVLEFSLEELEEPVQMDVEVQNTRPAAQWPSAVDVERDLRAVYRAMVEALSDAAQAVLDCKHWVKCWGLGDESDDQLRFLVEWRPQPWEAAELTRPLPSGEIIVVPVHTSIGELIIQAEHALRDTYCFFEEFQAETLDGITGEKWDPVVLGGAESGDTIGVHGHGADMETGLRCQGGLDTWEVRCVCGAQDDDGERMVACDACDVWHHTRCVGIADSEAVPPLFLCILCGGALLAAGPILEEALTLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6P5</accession>
    <name>A0A8I6Y6P5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        </source>
    </evidence>
    <sequence length="537" mass="59781" checksum="E7DB5617910515CC" modified="2022-05-25" version="1">MSRCFPFPPPGYEAKPRSEHTDLLKKENHKAKKHKKEKTDGGKRERKEKDRGHREDKHKKKHKRKKHKDRREKKERDKDKRPNLELGSRKNDDLDNRRPKQIVHNEAVKYSKHKDELATQITGQEGHANHAKSNTGKLLPRSIESFGVVGSKEKERTSISRVNGKSRPIAQHNHASEKGKNKTRILNGTSLQPGSAKKRSTGIHGSSGVGLQQENSKGILVTTTASEQKCRITPSANAAQRTEQVGQHPGGSSHSAYGKTDSVSTNFHFGMDKQSARGKSGADQGIAKVKEAEANHQKSVKDGDGSHDANQKVVKDRDRDRNVKKRKAKDGNEGKDREKRSAIDEQKCRELDGHEASKNYTHGLMDSAHLNGNKFTSDDVKKRKGLNANSPLHDHSMPMTKMPRTSPANHLCVNEEMLKHSQGTAHTLQVGTNPCEADMHEDSKEHNSAVSPSSYGSSEISMTPPHPDTKYLSQVYSVPAADDRSEYVDQDWLFSGDCVCVHQKTTKFEAAEAPQVWAEAQLIDSVDVVALPYVVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHU2</accession>
    <name>A0A8I6YHU2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123412839</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961720.1">
        <property type="nucleotide sequence ID" value="XM_045105785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHU2"/>
    <dbReference type="SMR" id="A0A8I6YHU2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0706130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0706130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0706130"/>
    </dbReference>
    <dbReference type="GeneID" id="123412839"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0585620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0585620.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0585620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0706130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0706130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0706130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412839"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE-LIKE PROTEIN CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE-LIKE PROTEIN CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035216943" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="30"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="493"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="800"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="800"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="816"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="521"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0706130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="854" mass="91523" checksum="3C7A07B820B05D1C" modified="2022-05-25" version="1">MLMSPRRLGLAINTAPLLLLLVAVSAAAAAPPPSPRQFSTVAISNTANSTLVCALIIDRVNEDGGGNSKLQCKSMPDGRVTSYPSADIPFNAIAAGRDFLCGLMAPVGGHAAMRWWSFSEEEEARRSRPIGRRIYWGTSLRAMSAGGPHVCGLSDDHEPTCWEWPGLSLPKGLDFSLIALGQDFLCGIVKGNDTAMTCYGGGMLQPPPFKGDGLAAPFQRVAAGRRHACAVDKLGGLVCWGDGNPTVRADQLPADMMAMALGRNTTCILAGNGTVRCWGVKVPEEYTHTTFVSIEADGDTVCAVMTINYSVVCWGNDGRFDGRHLVYNDTMPGACTPKSNCSCNILPDSGRLCGTGGGLAGEELAVCSPCSQALNASRIVVSNGNTAQLGDDGGDGKKNKKKLVVALGVASAGVAVIIVAGLTFYLLASRRSHKKAHDTVRLGESSSRRLCRDGRDVEIMVMPAPERSGLQPARPLGCEEFTLKDLSRLTNGFAEEAKIGSGSFGSVYRAKLPDGREVAIKRAERASTGARRRRRFDAERAFRSELRLLSRVNHRNLVSLLGFCEERGERILVFEFMPHGALHDHLHGEDAGSGHSPLFSSWEARLRVALDAARGVEYLHCYAVPPIIHRDIKPSNILLDDDWTAKVSDFGLSLVSGASASTSPTTTSGTAGTVGYMDPEYYRLQELTERSDVYSFGVVLLELVTGRKAIHRDQSQEGSGSPRNVIEFAVPAVEAGNIAKILDGRVPEPRGHEVEAVARVAKIGAECVRPRGRGRPVMSEVVAELEWAVTLCEECVVRSASGGRNSSSRNGGSDMSRSRSRSESEDRSPFHTREFSFGSGRVAAVGHARSYSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKJ3</accession>
    <name>A0A8I6YKJ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC123408493</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable ATP-dependent zinc metallopeptidase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957526.1">
        <property type="nucleotide sequence ID" value="XM_045101591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKJ3"/>
    <dbReference type="SMR" id="A0A8I6YKJ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751760"/>
    </dbReference>
    <dbReference type="GeneID" id="123408493"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0623600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0623600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0623600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0751760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408493"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019684">
        <property type="term" value="P:photosynthesis, light reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010304">
        <property type="term" value="P:PSII associated light-harvesting complex II catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.210:FF:000003">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF113">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="23">
        <location>
            <begin position="266"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="688" mass="72933" checksum="C6191E88D0B8F2B1" modified="2022-05-25" version="1">MAPPSLSSSHLLITASLPKPSSIRPPRLPVSSKPVPALLLALAASPAFPALAVDAPSPLPPQEAAPQLQMQAEAPTPAANPFADTLLTAPKPGSAAADIPDGGQWRYSEFLAAVKKGKVERVRFSKDGGMLQLTAVDGRRASVVVPNDPDLIDILATNGVDISVAEGDAAGPGGFLAFVGNLLFPFIAFAGLFFLFRRAQGGPGGGPGGMGGPMDFGRSKSKFQEVPETGVTFQDVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGPPGTGKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKAKAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTINQLLTEMDGFSGNSGVIVLAATNRPDVLDSALLRPGRFDRQVTVDRPDVAGRVRILEVHSRGKALAKDVDFDKVARRTPGFTGADLQNLMNEAAILAARRDLKEISKDEISDALERIIAGPEKKNAVVSEQKRKLVAYHEAGHALVGALMPEYDPVAKISIIPRGQAGGLTFFAPSEERLESGLYSRSYLENQMAVALGGRVAEEVIFGQDNVTTGASSDFMQVSRVARQMVERFGFSKKIGQVAIGSSGGNPFLGQQMSSQKDYSMATADIVDAEVRELVETAYSRATQIINTHIDILHRLANLLIEKETVDGEEFMSLFIDGQAELFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPM2</accession>
    <name>A0A8I6YPM2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123404077</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953941.1">
        <property type="nucleotide sequence ID" value="XM_045098006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPM2"/>
    <dbReference type="SMR" id="A0A8I6YPM2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0538770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0538770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0538770"/>
    </dbReference>
    <dbReference type="GeneID" id="123404077"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0448130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0448130.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0448130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0538770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0538770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0538770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404077"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152958" description="Subtilisin-like protease" evidence="11">
        <location>
            <begin position="26"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="49"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="125"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="640"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0538770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="79165" checksum="8F1C32FECE532CC9" modified="2022-05-25" version="1">MGSMMLRCSQLLLLVLLVNVKKVSCHEVEEEPSSTYIVLTDHLVKPSKYASMVGNNTDGILRHTYATVMHGFAARLTDGEAKRMATVPGVYRVYKDTVYDTQTTRSPWFMGLHDDFGAWPDSDFGDGVIIGFIDTGIWPEHPSFDHAGIGPVRSTWRGKCVGFNASLCSSSNKLVGARSFIFTHHDLVGAGDGDYPSPRDVEGHGTHVASTAAGSEVHSADLFKFAGGRASGVARKARIAMYKACLPIGCFSSDVAAAIDAAVSDGVDIISMSLGGPAQALYDDVLAVATFGAERRGVLVVLAAGNGGPEASSISNVAPWMTTVGAATTDRAFPATLRLGNGVVLTGQSLYNIPFSQSQPQLPLVRSDCGEDDLTPDRVMGKVVVCSGDADAWAGFYVERAGGAGMIAGDSKERFSDAVTAQPFTLPGLLLSSTDGKKLDDYMSSVAYPVASFNFTCRTVTGENRAPMVAGFSSRGPNPIVPEILKPDVIAPGVNILAAWSSAASPSGSDMDPRRVEYNIISGTSMACPHVAGVAALIKKRHPDWTPAMIRSALMTTAGPLDKNGRDMVDSGSPVGASATPLAAGAGLVIPRLAMDPGLVYNVEDYVDFLCTLNYTVEQMRLFVPELTKCARTIPGGVANLNHPSFVVVFDGRTGGLERTLTRTVTMVSACPESYNVTVAVPDGVKVAVTPAALEFRWPMEKRSYSVEFSSKARRAGEWDFGHIAWENRKHRVRSPVAFNWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQK5</accession>
    <name>A0A8I6YQK5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQK5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF101">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33562" checksum="7F46EDC3C12BF527" modified="2022-05-25" version="1">MMTRGQRRRLGLADPPSPCAGSGNPRKRPRQGLRPCTAVRLTGSLRRTGLREETEWRDWANLTTGIANDVAVRLLSLNVSEYLRFRAVCKPWRALTEDPRAHGVLDSRFRPHGWFTPSKRGEPPSRRRLLHTTAMARVTVHHQIFSTSRFLCVADGLLVMCDEATCTVRLLHPFTGVVAEFPDITDVGPHEGAEPSARLRMDAFKARFPGLGGGADPDAYLATDDYECGYPAERTVMDFASIDDSTSPPTLQLCVKTGKWLVISAKPGDEHWVSLSRLRGSERVMLHCALLRLVMVKSNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRM3</accession>
    <name>A0A8I6YRM3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1</fullName>
            <shortName evidence="11">Oligosaccharyl transferase subunit DAD1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123413647</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Endoplasmic reticulum membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the DAD/OST2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962517.1">
        <property type="nucleotide sequence ID" value="XM_045106582.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962519.1">
        <property type="nucleotide sequence ID" value="XM_045106584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRM3"/>
    <dbReference type="SMR" id="A0A8I6YRM3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0736140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0736140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0736140"/>
    </dbReference>
    <dbReference type="GeneID" id="123413647"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0610610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0610610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0610610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0736140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0736140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0736140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413647"/>
    <dbReference type="OrthoDB" id="445566at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0736140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12323" checksum="85BE569F53D3DA90" modified="2022-05-25" version="1">MPKAAGDAKLLIQSLNKAYAATPTNLKIIDLYVVFAVVTALLQVVYMGIVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKDNKEFKDLAPERAFADFVLCSLVLHLVIMNFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRV2</accession>
    <name>A0A8I6YRV2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123404445</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044954314.1">
        <property type="nucleotide sequence ID" value="XM_045098379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRV2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573570.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573570"/>
    </dbReference>
    <dbReference type="GeneID" id="123404445"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0475490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0475490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0475490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0573570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573570.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404445"/>
    <dbReference type="OMA" id="WFAVESP"/>
    <dbReference type="OrthoDB" id="2526284at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF64">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573570.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="582" mass="63126" checksum="0F76743F074B757F" modified="2022-05-25" version="1">MPRHSRCSRLSRISSIAGGVSVGALLLLAGGHNAYSRAPLFSWPLGHGAPFTLGADSPAPPPFPFAADFSPSPSPSPQYQYSSFRLSDDSLARRLLPLRLRAPQQDDAVLLPDQEVLLLDDAEPTGDAICAFQGGASSPARPLGRLPASGLHAYVCRLPEPAQSFQQLQAPLLLHSSTPSAAAAAPDSPSPSPSPGRALLNWSSDPIVFDSSLLDGGDVLVFAKGISHRQDLQCFYRYSDGADTILASSPAITSVQQVTRCPPAPTPMKSGGSTSGVLVTLGVTGEDPMPSLATFHRQQPESSSVTPQKSSVCACTMGRNIAKFLGEWALYHTAIGVDHFFIYDNGSEDNLAQRVAQLKSTGLNITTVPWPWTKTQEAGLSHCAATQQGSCTWMAVMDVDEFIFSTSWIGLEMPSKSLLDPVIAVDDTVGQIYLACYDFAPSGQTEHPPEGVCQGYTCRLKNPQKHKSLVRLDAVEPSLMNVVHHFKLKPGFKSIWTAFARVNHYKYQAWSEFKVKFKRRVSAYVADWKDPINLDSKDRAPGLGVDDKEPDGWAQKFCEVKDNTLQLLSARWFGVGFRNPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YT27</accession>
    <name>A0A8I6YT27_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YT27"/>
    <dbReference type="SMR" id="A0A8I6YT27"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0489320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF48">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="54"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="274" mass="30684" checksum="9C984FBA0AA28F7A" modified="2022-05-25" version="1">MNTALGAEYTREEVKAALDHIGDLKAPGPDGMSATVFKRHWHFMGDKIVEEVLKVLHGGDFPEGWNNTTVVLIPKVKNPKRIKDLRPISLCNVMYKLVSKVVANRLKIILPEVISENQSAFVPGNLITDNVLIAYEVSHYLMNKRNGRNGVAAVKADMSKAYDRVEWTFLEAMLGKLDFSTRWIDLVMKCVTTVRYQIKINGTLTNQFKPSRGLRQGDPISPYLFVICAEGLSALLRHAEEQGTLHGVKICPRAPCVSHLLFADDSMLLIRAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YTB7</accession>
    <name>A0A8I6YTB7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Nicotianamine synthase</fullName>
            <ecNumber evidence="2 6">2.5.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123405224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-thioadenosine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16481"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58249"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the nicotianamine synthase (NAS)-like family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.43" evidence="2 6"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YTB7"/>
    <dbReference type="SMR" id="A0A8I6YTB7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0474050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0474050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0474050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0571820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571820"/>
    </dbReference>
    <dbReference type="OMA" id="HINTFPY"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030410">
        <property type="term" value="F:nicotianamine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030418">
        <property type="term" value="P:nicotianamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004298">
        <property type="entry name" value="Nicotian_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266:SF10">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03059">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51142">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="267" mass="28553" checksum="CBFA72567129AB37" modified="2022-05-25" version="1">MREALIRLCSAAEGKLEAHYADLLATFDNPLDHLGLFPYYSNYVNLSRLEYELLARHVPGIAPARVAFVGSGPLPFSSLVLAAHHLPETQFDNYDLCGAANERARKLFGASADGVGARMSFHTADVADLTQELGAYDVVFLAALVGMAAEEKAKVIAHLGAHMVEGASLVVRSAHGARGFLYPIVDPEDIRRGGFEVLAVHHPEGEVINSVIVARKAVEAQLSGPQNGDAHARGAVPLVSPPCNFSTKMEASALEKSEELTAKELAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YU03</accession>
    <name>A0A8I6YU03_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YU03"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0603260.1">
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    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
    <sequence length="196" mass="23216" checksum="52DAC3EFB1A67B5C" modified="2022-05-25" version="1">MNLPCSDQSIRPRKMQSASLPRGVEAVRCWCGDVCKVKEVTDFSDWLGMKFFMCANYESDPPESISAYVRPPSPPPLCMYYRWIDTKMSDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYCVEQRAFLDEMIRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEAGDGKGKYPCWTQ</sequence>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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    <reference evidence="2" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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            <begin position="41"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="17506" checksum="B608F4A869585561" modified="2022-05-25" version="1">MRLRGHGVNDFPYDVRYESYIARLGLLPFVLQFKRTPPPVNHATLTALTDHWRPETHSFHLACGEDMAMISGLPINGFALTGHVSVFNSRDQVGFFNWCSAPPPLPGKADSSKVKHSWLKEARGQNSCPADADEMVVQQYARAYLWYVLKKVVFT</sequence>
</entry>
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    <accession>A0A8I6YUH4</accession>
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        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="8" type="primary">LOC123402948</name>
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    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the bHLH protein family.</text>
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    <dbReference type="SMR" id="A0A8I6YUH4"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:positive regulation of gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
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    </dbReference>
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        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
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        <property type="entry name" value="bHLH_dom"/>
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        <property type="entry name" value="bHLH_ZIP_TF"/>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF319">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="156"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="353" mass="37258" checksum="460D1D91C20EB5F1" modified="2022-05-25" version="1">MAHCGGGGGGPGPAASVESHFQGLLDDDDKYRALCGAFGYLQQQEWPDLSGACYAAFGAPPPPPAGTSNGAGNSFSRSGSASSGGGSTKRKPDAYLDAKDGCKRPRGKQQLRDPDEAGGAAKGRPEKPKACARKKPEAAAAGQKTDYIHVRARRGQATDSHSLAERVRRERISERMRYLQELVPGCDKVTGKAGMLDEIINYVQSLQKQVEFLSMKIAAANPVVNFNIVDDLFGGRRMSQPCGPAAALPAMTLPMHHGQLDPSCLQMSNTAMQQMQQPAAGFGLDMVLNHPYPLRSAAATPASASAATSVESCLDVNGAAAWDIASQNLFSGFDAQFQSVQSGCLLDNLKMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVN6</accession>
    <name>A0A8I6YVN6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123397188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044947771.1">
        <property type="nucleotide sequence ID" value="XM_045091836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVN6"/>
    <dbReference type="SMR" id="A0A8I6YVN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515040"/>
    </dbReference>
    <dbReference type="GeneID" id="123397188"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0427950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0427950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0427950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0515040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397188"/>
    <dbReference type="OrthoDB" id="730395at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000444">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001484">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF357">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="213"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="415"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="516"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="586"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="617"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="652"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="854" mass="93926" checksum="00E5018ED26CD101" modified="2022-05-25" version="1">MRRRVLLRLRPPARRRLCAALTTAASTTSAPPSAYRLLALLRGCVAPSHLPLGLRIHARAVTSGLLAAADGNVPAADALHTRLIGMYVLARRFRDAVAVFSSLPRAAASSALPWNWLIRGLTMAGHHRFAVLFYVKMWAHPSAPRPDGHTLPYVVKSCAALGALALGRLVHRTARTLGLDRDMYVGSALIKMYADAGLLGRAREVFDGMAERDCVLWNVMMDGYVKGGDVASAVGLFGAMRASRCDPNFATLACFLSVCATEADLLSGVQIHTLAVKYGLEPEVAVANTLVSMYAKCQCLDDAWRLFDLMPRDDLVTWNGMISGCVQNGLVDNALRLFCDMQKCGLQPDSVTLASLLPALTDLNGFKQGKETHGYIIRNCVHLDVFLVSALVDIYFKCRDVRMAQNVFDATKTIDVVIGSTMISGYVLNGMSEGAVKMFRYLLEVGIKPNAVMVASTLPACACMAAMKLGQELHGHVLKNAYEGRCYVESALMDMYAKCGRLDLSHYIFSKMSAKDEVTWNSMISSCAQNGEPEEALELFRQMSMEGVKYNNVTISSILSACAGLPAIYYGKEIHGIIIKGPIRADVFAESALIDMYGKCGNLELAFRVFEFMPEKNEVSWNSIISAYGAHGLVKESVSLLCRMQEEGFSADHVTFLALISACAHAGQVQEGLRLFQCMTEEHHIAPRVEHLACMVDLYSRAGKLDKAMQFIADMPFKPDAGIWGALLHACRVHRNVELAEIASQELFKLDPHNSGYYVLMSNINAVAGRWDGVSKMRRLMKDKKVQKIPGYSWVDVNNTSHLFVAADKSHPDSEDIYMSLKSLLLELKHEGYVPRPDICHIMQPDNITQVMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z4F1</accession>
    <name>A0A8I6Z4F1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uncharacterized protein ycf68</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ycf68 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z4F1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0790510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0790510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0790510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0790510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0790510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0790510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022546">
        <property type="entry name" value="Uncharacterised_Ycf68"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34890">
        <property type="entry name" value="ORF16-LACZ FUSION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10839">
        <property type="entry name" value="DUF2647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0790510.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="129" mass="14571" checksum="2085EA25A2355788" modified="2022-05-25" version="1">MAYSSCLNRSLKPNKLLLRRIDGAIQVRSHVDLTFYSLVGSGRSGGGPPRLLSSRESIHPLSVYGELSLEHRLRFVLNGKMEHLTTHLHRPRTTRSPLSFWGDGGIVPFEPFFMLFPAVWRKQQSIGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z8J3</accession>
    <name>A0A8I6Z8J3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z8J3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0615470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0615470.1.CDS.1"/>
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        <property type="entry name" value="vWFA_dom_sf"/>
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        <property type="entry name" value="OS06G0652100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31373:SF28">
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            <end position="182"/>
        </location>
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        <location>
            <begin position="184"/>
            <end position="410"/>
        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="422" mass="47783" checksum="4453702241CEFC3A" modified="2022-05-25" version="1">MTAEVFADLLAEDMKKLAAGNMDLSLAGKWCPSVDSCYDRSTLICEAIARRLFPKGSAPDLPEDLPDAHYAYRVRERLRKEAYVPLRHALRLPEIFMSAGEWGKVVYTRVSSVAMKNYKEHFAYYDNKRFRRYLAEVKAGNVKIAAGALLPHEILASAKFDPEVANLQWKRMVDDLLALGKLNNCLAVCDVSGSMSGLPMDVCVSLGLLLSELCDEPWRHRVITFSMRPQLHHVAGDTLWDKAQFIQRMDWQMNTDFQAVFDQLLHIAVAGNLAPESMVKKVFVFSDMEFDQASASPWETDYEAITRKYTDAGYADAIPQIVFWNLRDSRSVPVTSEQKGVALVSGFSQNMLKLFLGPEEEEQKVEDKEKEEEEEQNETEEEQSKGGEKELKKGKEEVTLTPRSTMEKAIAGPLYEKLVVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZA40</accession>
    <name>A0A8I6ZA40_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123411366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960229.1">
        <property type="nucleotide sequence ID" value="XM_045104294.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2020591at2759"/>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035186881" description="Thaumatin-like protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="319"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0687480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="33196" checksum="F58C9E0959F712EA" modified="2022-05-25" version="1">MAQPSLLLGSPASIVLLLLSFFQGPVAGAITFTFANRCTDTVWPGLLSGSTSPPLETTGFALSPGQSRSLYGPTGWSGRFWGRSGCNFNGGKGTCVTGDCGSGEIECRGAGATPPATLAEFTLDGDGGKDFYDVSLVDGYNLPMLVQPSAPGCSNTGCLVDLNERCPSELRSGGGLACRSACEAFGKPEFCCNGAYGNPDTCHPSQYSQLFKSACPKSYSYAYDDATSTFTCNHTDYTITFCPQSNPYSGKPNKHALRKPSHEQLEDDVWLASLKKTSGAGGGTMASWSATGGFQAALAIAVVVVLATAQGCNPPFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZFT5</accession>
    <name>A0A8I6ZFT5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZFT5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="125"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="221" mass="23863" checksum="313E62C1EC0E3C98" modified="2022-05-25" version="1">MGSNDWVKFVADFNLGVSALVVFDTSKRTAEAYVAHLGFAHLANDGDPGEDIVANEDEVGEGAGVEVFNIESDEEVLNDDHEKADARELVYTHGCVLSDAEGSLLDVVLTNNDGYIGSSLIHRLTTTNISKKTMKIPRKVVEQVNLPPVGVAGICIGAGECMNVAYHTDCDGRMVFTNQWGVFATQTNLQEGDAIVFSFKPSNAGDMNIICVVHILRMPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B196</accession>
    <name>A0A8I7B196_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B196"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0067520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="20753" checksum="338069705E1BFB6C" modified="2022-05-25" version="1">MWHYTGPEDSTRTNVVGILEEKVTTWVLQITGPCENPKGARRVTPYSADNPPPNQAWTNWFSPVSNGDPEEEEEEGSQEGSVESAEYVSESGESEEEDNEEEEEDEERDSPPPRPEHRNKRRHEPAAPSAPPASSSAPPAAPVAPSVRGTKRARDAAAEPAGQSSRAPKPSGPKPRKALPRMRVAIPVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B251</accession>
    <name>A0A8I7B251_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC123424621</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044964197.1">
        <property type="nucleotide sequence ID" value="XM_045108262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B251"/>
    <dbReference type="SMR" id="A0A8I7B251"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0108930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0108930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0108930"/>
    </dbReference>
    <dbReference type="GeneID" id="123424621"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0089440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0089440.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0089440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0108930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0108930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0108930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424621"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000112">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="334"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0108930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="658" mass="73366" checksum="098A285EAA4C649E" modified="2022-05-25" version="1">MLLLEPYHFVVLLLLLVAAGRFLVASGSSGDDGRPFVYNGFTGVNLTLEGAAFMQNGLLRLTSGTGKGQAFHPSPLPFRTASNATGLVRSFSTTFVFTIFSQYDIDADLSSDSMGFFVSESKDVYLGFGYRGYTDTSHRFFAVEFAFEDERSDVGIYLNSSASDSSNAGYYADGTEIFRYLSLMSGKAVQVWVDYDGRATEITVTMAPLGMARPQRPLLQTTNINLSDVVQSTAYVGFSSARSDIFSTRQLILGWSFALDGPAPALDISVLTAMSMSLKIVLVIASVALVLVGMGVYIFVRRRLKYSEVHEDWEVPFGPHRFSYKDLFHATEGFSDKNLLGRGGFGSVYKGVLRKPDMEVAVKRMSHDSRQGVKEFIAEVVSIGRLRHRNLAQLLGYCRRKGELLLIYEYMENGSLDKYLHNIKGPVLDWPQRYWIIKGVASSLFYLHEKWEHVVIHRDIKPSNVLLDSQMNGRLGDFGLAKIYDHESAAPATHVAGTIGYLAPELARTGRPTPFTDVYAFGMFLLEVTCGRKPIFTDKQNNRLLLVEWVLEHHRDGSILDTVDPRLQGEFNMEEVTIVLKLGLICTYPLPNMRPIMRKVMQYLVHNQPPPDLSPAYISYMSLMQNQGFNSDNMNTTCSEATTSVATVSSATILRDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3K1</accession>
    <name>A0A8I7B3K1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EndoU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123427392</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967357.1">
        <property type="nucleotide sequence ID" value="XM_045111422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3K1"/>
    <dbReference type="SMR" id="A0A8I7B3K1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0185230"/>
    </dbReference>
    <dbReference type="GeneID" id="123427392"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0153360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0153360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0153360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0185230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0185230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427392"/>
    <dbReference type="OrthoDB" id="430326at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF11">
        <property type="entry name" value="URIDYLATE-SPECIFIC ENDORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="EndoU" evidence="12">
        <location>
            <begin position="202"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="56"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="475" mass="54819" checksum="AE0FB8094F72621E" modified="2022-05-25" version="1">MEGLIKGLAKAAIGALEGGDDRRERERERERDEDREAPRRHRPARDAEEDEDEEGREQRDRSTWAEVVSEKKGGEPQEERRGSRPSRREERREDEGWERNQGRKQHPSSHGGSHSQHQGEGRSDGGSRLPQQQAPAYRRQQREEEEEKNDEGWETVGEKKHHGRPQQCEAWNGYKRPPSEQEYSEDVGQIHHGLNVEPTREELNSLTEACSRLWELDMNRLVPGKDYRIECGEGKKVYQKDDMASENLFSWLGEDVLTKPTYSRFCALLDNYNPHQGYKEVVTQQDKHEEAAFIEEIARTAPIKYLHRYLVLKGVASQDYDDFKAMLTSLWFTLYGRCGNSSCSSAFEHVFVGEIKEQRQGETEVSGFHNWIQFYLEEAKGNVDYQGYIFPRRRGESPDSETQLLTVQFEWHGVLKSVSSSLIGVSPEFELALYTLCFFMGGEDNRVDIGPYTVNVKCYRMGNNKIGSVFPIAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    </protein>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0274660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="104" mass="12463" checksum="044C084B51A6886F" modified="2022-05-25" version="1">MNFPSVLTREEAKAWMPSPWVIRAKGSRIQARWERRLLIVKTDEQAMEVWRQRFLQDVLEEREFWARQRTVRAAERVERHERKAAVVAQCDLGHASTWSTDDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B834</accession>
    <name>A0A8I7B834_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123428754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044968847.1">
        <property type="nucleotide sequence ID" value="XM_045112912.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8I7B834"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195150.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195150"/>
    </dbReference>
    <dbReference type="GeneID" id="123428754"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0161930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0161930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0161930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0195150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428754"/>
    <dbReference type="OrthoDB" id="670255at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF177">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="107"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="27453" checksum="EF292540D3399853" modified="2022-05-25" version="1">MEQHFTVTFPPGSYHHHHHRYDAVAAAAPSPDFDTSDMDDVAFLSTLLASTDHASCSDPSLSSSPSSSDMTGVGDAAPHVPAGSSSSSSSPKRRGHHAPAGMPIGKDFIGVRTRPWGKFAAEIRDSTRKGARVWLGTFGSPEAAAMAYDQAAFSARGDAAVLNFPVERVRESLRALALGAVVGSPVLALKRRHRMRRRSPNKKPVKQPRVAAVKEDARPATAGVVVLEDLGAEYLEELLWVSEPPMDHLHGTATASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9E7</accession>
    <name>A0A8I7B9E7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of target mRNAs. Binds the APUM-binding elements (APBEs) in the 3'-UTR mRNA sequence of CLV1, PNH, WUS and FAS2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9E7"/>
    <dbReference type="SMR" id="A0A8I7B9E7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0492170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0492170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0492170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0492170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0492170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0492170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF123">
        <property type="entry name" value="PUM-HD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="661"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="681"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="717"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="753"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="825"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="860"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="896"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="932"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="244"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="619"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0492170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1012" mass="110559" checksum="4A4B546B81B61D58" modified="2022-05-25" version="1">MHLLTLLKTEFGWEFSTERLHLFFRTMVEGSALPLIGGTGAGDWQPSKDLSSYDPLPASLSGEELAFDVNDGAIHGSWRDAAPNRSGSAPPSMQGSLAELGHLISRHSGDLEATLRNLSSGAVSSESEELCSDPAYLKYYGAKVNLNPRLPAPHVSRENQRLMNRFSKPGERRPLYFDDSSSDRSLTHRSTLSTHKEEPEDDGSSKLDSSSAEDARCRNDQSTSSIECHSSKLVGLMQENFPRSTDLYNNSSGPSNTNSGDGESVCCISSSDLNGFPSDMPQRPRGPIGSPVRNTLSSNSVAASSPLTSSSSVNSIMGPSQQGSSSTEIKSGGAMRAILNGVDSSMKNLKISLDTQDSAHVKQQRQNNVFLQNGPLHGDPTQMIPQGINLPQVPFVNNFSHAHMNLYSGDIQLMSQHGMPTPFYTQSPYYQNPQLPGVLMPPYGIDGHGLPNSFLPPFMTNFAPQLPVMTPSDTPLTPTFSGRVAGFPSTGNIAAGTEFSNPFKIDEQLRVTMPPSVPDLSLIHYFQQPSIYHYGLGNPYDTVSPSSNFVGNFTAAFGPEKVMPGIMHQSGQKFQFPNTGACSSPTARKQGSYVGNHPGTSPYISIPMPYPTTPVFHGQPSSGTYPCDRRNNAPGFQSPSKTTGLSPGIQEQRAREKSGPNTHFFVGEVRSNKAHMVELSDIKGHIAEYSSDQDGSRFIQQKLENGTTEDKALVFDEILPHASSLMTDVFGNYVIQKMFEHGDSEQRRDLAKKLAGHVLNLSLQIYGCRVFQKALEAIELDQKIELVCELDGHVLECVRDQNGNHVIQKCIECVPVEHIGFVVSAFQGQVATLAMHPYGCRVIQRILEHCSNGSDGIIDEILQSACTLAQDQYGNYVTQHVLEKGKSNERSQIISKLAGQVVAMSRNKFASNVIEKCFQHGNIADRDLLIKDILEQTEGNNYLLVMMKDQFANYVVQKILVTCSGQQRDALVSRMKGHLQALRKYTYGKHTASRIERFSGDGAAPRGSQAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAG4</accession>
    <name>A0A8I7BAG4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC123444564</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Involved in resistance response to the pathogenic oomycetes Phytophthora infestans and Phytophthora capsici.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Promotes hydrogen peroxide H(2)O(2) production and cell death.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with ABCG40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044977267.1">
        <property type="nucleotide sequence ID" value="XM_045121332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAG4"/>
    <dbReference type="SMR" id="A0A8I7BAG4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0293500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0293500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0293500"/>
    </dbReference>
    <dbReference type="GeneID" id="123444564"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0244130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0244130.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0244130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0293500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0293500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0293500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444564"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="L-type_Lectin-RKs"/>
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        <property type="entry name" value="Legume_lectin_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    </dbReference>
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        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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    <keyword evidence="14 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0611">Plant defense</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 25" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035202563" description="non-specific serine/threonine protein kinase" evidence="25">
        <location>
            <begin position="26"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="340"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="648"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="652"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022734"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00058054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063357"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0293500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="672" mass="73185" checksum="9B7300E7AC09F843" modified="2022-05-25" version="1">MGISVAVPSLLLLSAALLLPPAAAGFSFTYNFTAVSDSAPSGISFQGDAFFNKFIRLTRDERVGPLTSSAGRAFFSRPIPLFDPVSRRPASFASAFSFSISAPEPSAASGDGLAFFLSPFPSVLPNRSAGGLLGLFNSSARNGGRSLVAVEFDTYRNDWDPSDDHVGIDLGGIASVATADWPTSMKDGRTAHARVAYDAEAKNLTVALSYGDAPPTDVLLWYAVDLREHLPDSVAVGFSAATGEAAELHKVLYWDFTSSVDSKEQTVMLWVILGLCGFLVVLVGAGVVWFVKEWRKTGECVPYVDIDDAMGYDELTDEFIVQSGPRRFRYAELVAATNNFSEQRKLGQGGFGAVYRGFLKELRQEVAIKRVSKGSTQGRKEYAAEVRIISQLRHRHLVRLVGWCHEHRGDFLLVYELMPNGSVDQHLYGKGVHLTWPTRYDIALGLASALLYLHEECLQCIVHRDIKPSNVMLDATFSAKLGDFGLAKLVEHGSQPYTTVLAGTLGYLAPECLTTGKASRESDVYSFGVVALEIACGRQPSEPTAEPSKARLVPWVWELYGKKALLEAADWRLKGEFDEKQMEHLMVVGLWCAHPDYTHRPSIRQALNALKFEAPLPVLAPKMPVPTFFPLPDLAALVSSVGGAQNPGVTECESSGANAGQRSSVRDRLLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDH2</accession>
    <name>A0A8I7BDH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123412632</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044961517.1">
        <property type="nucleotide sequence ID" value="XM_045105582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDH2"/>
    <dbReference type="SMR" id="A0A8I7BDH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657520"/>
    </dbReference>
    <dbReference type="GeneID" id="123412632"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545680.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412632"/>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000811">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
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    <feature type="chain" id="PRO_5035259218" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="32"/>
            <end position="688"/>
        </location>
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        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="360"/>
            <end position="644"/>
        </location>
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            <position position="389"/>
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        <ligand>
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        </ligand>
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            <dbReference type="ARBA" id="ARBA00004479"/>
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            <dbReference type="ARBA" id="ARBA00008536"/>
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            <dbReference type="ARBA" id="ARBA00010217"/>
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            <dbReference type="ARBA" id="ARBA00012513"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657520.1.CDS1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
    <sequence length="688" mass="76340" checksum="A5E7C5E214AF1DAC" modified="2022-05-25" version="1">MEHQSTLGFSLQLHILLSLALSLASFTTVTATSEEEGQFVYSGFSGSTDLTLDGTATVTPDGVLKLTNGTVHLKGHAFYPTPWQFSKSPNGVVQSFSIIFVFGMVPVYSDQCTDGMTFFISPTKDFSGAQTSQYLGLLNITNDGKSSNHIFAVELDSSQNTEFKDIDDNHVGININSLTSTEARPAGFYDDKNNGVFNSLSLVSRKEMQVWVDYDGDTTQINVTLAPLKVAKPLMPLVSTTKNLSTVLQDQSYVGFSASTGPIDSLYCVLGWSLGINRPAPDIDITKLPKLPRIGPKPQSKVLEIVLPIATAIFIFLVGTTIILLVRRRMRYAELHEDWEDEYGPHRFSYKDLFHATQGFHDANLLGRGGFGKVYKGVLPVSKLEVAVKRVSHESKQGIKEFIAEIVSIGRIRHRNIVQLLGYCRRKGELLLVYDYMSNGSLDKYLYLQEEEEHKPTLDWARRFEIIKGVASGLFYLHERWEKVVIHRDIKASNVLLDSEMNGRLGDFGLSRLYDHGTDPQTTRVVGTMGYFAPELARTGKATPLTDVYAFGIFILEVTCGRRPIIDSNQAEEVGSDILIDWVVEHWSKGSLADTLDMRLQGSNCNLDEVCLALKLGLLCAHPFGNVRPSMRQVMQYLDGEMLLPELMPTNVSYSMMAVMQNDGFSQYTSLTDTVSSNGVTSSLSTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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    </reference>
    <reference evidence="3" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="335" mass="35769" checksum="BAB78F67F3DB34B0" modified="2022-05-25" version="1">MMRNGKKREDLDDLHLSNKRKRTIELNDDEEEDDAPSDDSKRSPTPNSVSYSKPKRPNGCKKDKTEKKKRKGDDELTNAMETIVKARKEANKVRKMARNQDVAAEERRLEAEERRVAAEERKVAIEERKVGMEERAKLLEWEKHLFFLDTSLFNDVQKEYVNLAREEVLILKRAMIRSMGGGGLGAMGGGGLGAMGGVGLGAMGGGGLGAMGGMGGFGAMGGMGAPPAAMGGIGGFGAPSDAMAAMEGMSFASLMGGMGAPPAAMGGMGAPPAMGGRSFDVPPHTHSHEDAVEDLANTVGASRDAVRDEVREEDSSSEVEESSSEDEDEDEEDDD</sequence>
</entry>
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    <name>A0A8I7BEG6_HORVV</name>
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        <recommendedName>
            <fullName evidence="8">RNA-binding protein 25</fullName>
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        <lineage>
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    <reference evidence="7" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
        </source>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045105377.1"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0692740"/>
    </dbReference>
    <dbReference type="GeneID" id="123412430"/>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA Binding Motif protein homolog"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
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    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="PWI_dom"/>
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        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034268">
        <property type="entry name" value="RBM25_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053294">
        <property type="entry name" value="RBM_PWI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47334:SF2">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47334">
        <property type="entry name" value="SPLICING FACTOR PWI DOMAIN-CONTAINING PROTEIN / RNA RECOGNITION MOTIF (RRM)-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="209"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="PWI" evidence="5">
        <location>
            <begin position="807"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="302"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="625"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="736"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="422"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="737"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0692740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="904" mass="101894" checksum="6FB7C655BFFA6DEA" modified="2022-05-25" version="1">MAAVAPTHDNLDPPPSTPPSTATPPQATATATATSTPPNPATPNPPASNPTMSSPNPNPVPASAPAPPVVMPLMSPAPVSFAPSFRPLGAPPPPQVQQYGAIRNPGYLMGQQMQPPGVHHVMRPPTMYAPQPGPYMQQPGVAVPPGVHRYPGPYTMMRPMFPPRPMPPVGVVQIQRPAVIPGIRGAPPMVAPPARPPAPAVTTVDKPPTAVYVGKIAPTVDNGLLLSLLRICGPVKSWKRTQNPSNGKPVAFGFCEFESAEGILRATRLLNKLNIDGQELVVNINDATKEYLKKHVEEKKKVHENAKKEEDEGGDGTSAVAENESFKLISGKTDETEDSGDKDSEENTKKFGIVTNEDSEADKAAAEQIHSMIEEWLKTRPLPPPPPPPPVQPSAEISSKHNNGESVVDMAMTDSEDNNEDYIDKRTVSETEKAETDSLDKRKDREHDKEKQEKEKDLQRYERERERERVRRDRDKEHKHREVERLYKDHLKEWESRERDKEYQRQYEKDREKEKERERKREIMKQEDDSDEEDSRKRRRRGSNALEERKRRRQREKQEDLADKLKEEQEIAEARRRAIELQQQADEAAAAESAIFMEVDGDDWKETDAQNKPIISDDDNVFSIANGVDADDGKSNKDCNGDETSTVPGQILDIKQNSNAPAKKLGFGLIGSGKRTSVPSVFAEEDDDDNKDKRIRPLVPIDYSTEELQSVEADSYADQPNIVAAAEFAKRILVSNQKEEQPETERSRRAIDRSTLRDKSRIDEDGARLSDDRREIMPDRDKDKPKLENKKILDAKQLIDMIPRTKEELFSYDINWAIYEKHELHDRMRPWISKKIIEFLGEEESTLVEYIVSCTKDHVHASKMLELLQSILDVEAEMFVLKMWRMLIFEIKKVEAGLSGRGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFV6</accession>
    <name>A0A8I7BFV6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U3 small nucleolar RNA-associated protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123394955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044945795.1">
        <property type="nucleotide sequence ID" value="XM_045089860.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945796.1">
        <property type="nucleotide sequence ID" value="XM_045089861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFV6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530580"/>
    </dbReference>
    <dbReference type="GeneID" id="123394955"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0441390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394955"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001559">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="6">
        <location>
            <begin position="181"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="5">
        <location>
            <begin position="469"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="648" mass="74334" checksum="75CF0BF97C652F51" modified="2022-05-25" version="1">MGKLKPAPPTATPRGQKRRREKPKRQVLDDDLPDSSAGIISAKAVRREKPSNESPQGLHQSDDDDSDDCPVSSEDEAHDMISNKNDAVDETEPSCSFQRHVTHVLENDEVNALVNRKSKFKWEMPAEDIRKSKWVGTGEQIKGASDDPFVDVKGKLKDHWQKILSDDLNYRSRFFSLCNSYRDIMHCNKKPFYLKGSKVDSSTMDAYLMHALSHVHRTRDVVIKNDANLRNDANSDVLGDDSYRDQGFTRPKVLFLLPLKSIARRVVKRLIQLSPLAQKDNSMGQFKKEFGDSDDELEEPNSSKPTDFNLLFAGDVEDHFLFGIKFTKKSVKLYSNFYASDIIIASPLALKLKIDGGEVTQKKGRPKENDSDFLSSIEIVVVDYADVISMQNWSHLHAVLEQLNHLPSKEHVTNVMRIRPWYLDEQARYYRQTIILSSYLTPEMNALFNGLCLNYEGKVKLVTEFTGVLPKVQLEIRQVYERFDASSISELDDARFEYFCTKVYPKIQESDEGGVLLFASSYFEYIRLSSFLKSQDASFCRIGEATSQQDISRARLWFFEGQKKILLYSERSHFFHRYKIRGGHHLVIYSLPGRKDFYPELVNMLGESANPRCNVLFSRLDLLKLERIVGTSSARRLISSDKDMFVFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BH71</accession>
    <name>A0A8I7BH71_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SPT2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123403498</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953363.1">
        <property type="nucleotide sequence ID" value="XM_045097428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BH71"/>
    <dbReference type="SMR" id="A0A8I7BH71"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0601950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0601950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0601950"/>
    </dbReference>
    <dbReference type="GeneID" id="123403498"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0499110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0499110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0499110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0601950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0601950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0601950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403498"/>
    <dbReference type="OrthoDB" id="6259853at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691:SF8">
        <property type="entry name" value="PROTEIN SPT2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691">
        <property type="entry name" value="YEAST SPT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="131"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0601950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="53271" checksum="5CD4494DBB795EE6" modified="2022-05-25" version="1">MSGYDDDNETNLVEDEYDDLDDFIVGSDDEGDNVAEEEEDELPEAEEVEAEEEYEEEEEEEPPVGTQEILSFREQLKAKLRKQHQSSGADADGNPSCSSSDQPSVRSRFGTFFGPSTPVLAPRLMEAGCSSMMKENQDVPFRKHAAPSSASKTQQSASTHEQKPKIVPQVKRKVDTLRENRDYSNLFSDDADTPPLTKEDAEKKPVMDPKSDVQAHRMKAMQSTARNNKVPTSHTARPSKDHGSIQRRVENKVFSQVKKEPLPNGRKPNASARNGSRSANGKARPGLQPSSNGQNPQCSIQSKSPQVLPASQRQQQRPLKPQGPRQQNHVPPSPQVRRTNLSVQGQQPAHKGSALPRDRTKLGQKQLAPSSRPKPSPTSAVYGDPAKKKGGAKRKVSDAEKVRQMVRDVLNYDPNKYSHKDKDDDSDMEANYASIQMEERRSAKIARKEDEEEYRLLQEEEQRQRAKKKKQRKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-11-24" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>D0EL83</accession>
    <name>D0EL83_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Bell-like homeodomain protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Bel2</name>
        <name evidence="13" type="synonym">LOC123444896</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="Theor. Appl. Genet." volume="121" first="705" last="715">
            <title>The genetics of barley low-tillering mutants: low number of tillers-1 (lnt1).</title>
            <authorList>
                <person name="Dabbert T."/>
                <person name="Okagaki R.J."/>
                <person name="Cho S."/>
                <person name="Heinen S."/>
                <person name="Boddu J."/>
                <person name="Muehlbauer G.J."/>
            </authorList>
            <dbReference type="PubMed" id="20407739"/>
            <dbReference type="DOI" id="10.1007/s00122-010-1342-5"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="GQ853057">
        <property type="protein sequence ID" value="ACX35970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359573">
        <property type="protein sequence ID" value="BAJ90782.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D0EL83"/>
    <dbReference type="SMR" id="D0EL83"/>
    <dbReference type="PaxDb" id="4513-MLOC_66099.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0251940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0251940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0251940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302040"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011058_3_0_1"/>
    <dbReference type="OMA" id="AGGQMYH"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="D0EL83">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000117">
        <property type="entry name" value="BEL1-like homeodomain protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00574">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="342"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="344"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="418"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="510"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACX35970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ90782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="611" mass="66033" checksum="0A98F45C60695C45" modified="2009-11-24" version="1">MSSPAGGYGGAEAHHHGHMLLHSHAHHMAAAAAASGGQLYHVPQHSRREKLRFPPDAAAEDSPPTPLAPHHQHHQAGAWPPPAFYSYASSSSSYSPHSPTVPQGQQLVLNGLTAQQVTAQQFPHIPTHNFSLSLSSASSNPATAPPTPRKQQEPGGAGPCGPFTGYASVLGRSKFLVPAQRLLEEICDVGGAAAHADRSLPDEGLLDADTMDVADDELDAAGPMYGAEQQWKKTRLISMMEEVCKRYRQYYQQVQSAIASFETVAGFSNAAPFTALALRVMAKHFKTIKEMILSQLRNTSKMPVKGSSMSKDITIFGLGGGGGAPVGGFQRGSSVNGFGQPHNIWRPQRGLPERSVTVLRAWLFEHFLHPYPTDGDKQMLAKQTGLTRNQVSNWFINARVRLWKPMVEEIHNLEMRQVHKQSPHDNGSQHGVHGHAHQPSSQQQQQQRSGKRSEPCDSHLGQCSGVTRNHHHHSNPAASSHGGGFPDDLSQMSHSMQQGQVTFAGYGALPSQSQQHQHQHQHSSMASPQHPHHQHHVGAAGAGNGGGVSLTLGLHQNNRVCFGEPLPANLAHRFGLEDVVSDPYVMGSFGGGQDRHFAKEIGGHLLHDFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQ79</accession>
    <name>F2CQ79_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123448355</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AK353781">
        <property type="protein sequence ID" value="BAJ85000.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981138.1">
        <property type="nucleotide sequence ID" value="XM_045125203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQ79"/>
    <dbReference type="SMR" id="F2CQ79"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361130"/>
    </dbReference>
    <dbReference type="GeneID" id="123448355"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0301010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0301010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0301010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0361130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448355"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000028">
        <property type="entry name" value="3-ketoacyl-CoA synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="13">
        <location>
            <begin position="91"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="14">
        <location>
            <begin position="397"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAJ85000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="514" mass="56579" checksum="92E8357C1C5F01DA" modified="2011-05-31" version="1">MSAAAQETTTGGGESKHKHHGPRRPGYLKLGYHIVVSNAVYVLLAPFAVALALRASRLTPSDLAAARAYLVANLQLAVSLVSVATTLATVYLARRPRAVYLLDLACYKPGPEHVVTRETFMEQSNKAGVFTDDNLAFQRKILERSGLGQETYFPAAVLNSPPNPCMAEARREAEAVMFGAIDSLLANTGVGARDIGVVVVNCSLFNPTPSLSAMVVNHYKLRGNVASYNLGGMGCSAGLISIDLAKQLLQVHRNSYALVVSMENITLNWYWGNDRSMLMSNCLFRMGGAAILLSNRGADKRRSKYQLVHTVRTHHGADDRAYRCVFQEEDKAGRVGVALSKDLMAVAGEALKTNITTLGPLVLPMSEQILFLTSLIGKKIFGLKMKPYIPDFKMAFEHFCIHAGGRAVLDTIEKNLELSDWHMEPSRMTLNRWGNTSSSSLWYELAYTEAKGRVRRGHRVWQIAFGSGFKCNSAVWRALKDIHPSKEACNNPWIEEIYRFPVVVPKVESVVSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSP9</accession>
    <name>F2CSP9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123406275</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK354651">
        <property type="protein sequence ID" value="BAJ85870.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955695.1">
        <property type="nucleotide sequence ID" value="XM_045099760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CSP9"/>
    <dbReference type="SMR" id="F2CSP9"/>
    <dbReference type="PaxDb" id="4513-MLOC_16684.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623490"/>
    </dbReference>
    <dbReference type="GeneID" id="123406275"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0517380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0517380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0517380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0623490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406275"/>
    <dbReference type="eggNOG" id="ENOG502QT89">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CSP9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04692">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000056">
        <property type="entry name" value="Nudix hydrolase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF9">
        <property type="entry name" value="ZN-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="34"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ85870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="776" mass="87054" checksum="C37781AD389FBE28" modified="2011-05-31" version="1">MAAQAPEERLDVLTAAGDKTGVSKPRSEVHRDGDYHRAVHVWIYCESTGELLLQRRADCKDSWPGQWDISSAGHVSAGDSSLSSARRELQEELGIKLPVDAFELIFVFLQECVINNGTYTNNEYNDVYLVTTLTPIPLEAFTLQESEVSAVRYMHRDEYKSCLAAESGEYVPYDVNGQYGQLFSIIEERYKDNTESRSLTLQKQISRYAPIHLEPELTTLSEGDREALGYILKASMVIDEIFYEQVWNSNTMLRDWLKAHADSSSLDTLKWAYYSINKSPWSCLDENKAFLSTADSAVKLLTDATKPISGWKGLEYRAAFPLDKPRGANFYPADMDKMEFDLWKSGLTDKEQKDATGFFTVIKRPDALLTTSVAQSDGPNQTNTSDDLFIVPYSKEYKTSLEKAAELLLKASDCSDCPSLKNLLKTKANAFLSNDYYESDIAWMELDSNIDITIGPYETYEDGLFSYKATFEAFVGVRDEVATSQVKLFGDQLEDLEKNLPLDNIYKSDNVSAAPIRVMNLLYNSGDVKGPQTIAFNLPNDERIVNERGTSMVMLKNVSEAKFKHILKPIANACIREEQKEYVDFEPYYTHIVCHECCHGIGPHSITLPGGKKSTVRMELQECHSALEEAKADIVGLWALNFLINKGLLPKSLSKSMYVSFLAGCFRSIRFGLEEAHGKGQALQFNWLYDKGAFILHSDGKFSIDFTKVEEAVESLGREIMTIQAKGDKPAAQSLLQSRATLTQPLRVALEKIEHMQVPVDIAPIFGTASKLLANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F2CUP0</accession>
    <name>F2CUP0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDP-D-glucose phosphorylase 1</fullName>
            <ecNumber evidence="5">2.7.7.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123453136</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specific and highly efficient GDP-D-glucose phosphorylase regulating the levels of GDP-D-glucose in cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GDP-alpha-D-glucose + phosphate = alpha-D-glucose 1-phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30387"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:62230"/>
            <dbReference type="EC" id="2.7.7.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GDPGP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.78" evidence="5"/>
    <dbReference type="EMBL" id="AK355342">
        <property type="protein sequence ID" value="BAJ86561.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985837.1">
        <property type="nucleotide sequence ID" value="XM_045129902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CUP0"/>
    <dbReference type="STRING" id="112509.F2CUP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0450950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0450950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0450950"/>
    </dbReference>
    <dbReference type="GeneID" id="123453136"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0373510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0373510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0373510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0450950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0450950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0450950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123453136"/>
    <dbReference type="OrthoDB" id="417175at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CUP0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080048">
        <property type="term" value="F:GDP-D-glucose phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026506">
        <property type="entry name" value="GDPGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058865">
        <property type="entry name" value="GDPGP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058866">
        <property type="entry name" value="GDPGP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884:SF8">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26216">
        <property type="entry name" value="GDPGP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26217">
        <property type="entry name" value="GDPGP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDPGP1-like N-terminal" evidence="14">
        <location>
            <begin position="69"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="GDPGP1-like C-terminal" evidence="13">
        <location>
            <begin position="249"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF26216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF26217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAJ86561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0450950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="432" mass="48020" checksum="60B67350E9102888" modified="2011-05-31" version="1">MLDMKLTIKRVPTVVSNYQEDAGEQPRGGCGRNCLGHCCLPVSELPVYAFKANSTKLPLQKDAVPTDFFINLLLGQWEDRMTQGLFRYDVTACETKVIPGNLGFVAQLNEGRHLKKRPTEFRVDNVLQPFDSSKFNFTKVGQEEVLFKFENSGTDDSYFLRSAAITVVDRAPNVVAINVSPIEYGHVLLIPRVLDHLSQMIDQESFLLALHMAAEAASPYFRLGYNSLGAFATINHLHFQAYYLTVPFPVEKAATQRISLPEGGMKSGVKVSKLMNYPVRGLVFEEGNTLNDLANVVSSACIWLQDNNVPYNVLISDSGRKIFLFPQCYAEKQALGEVSQELLDTQVNPAVWEISGHIVLKRRDDYEEASEASAWRLLAEVSLSEARFEEVKAYIFDATGLVQLQVEETNGIIYAPVPVSPSAVAEGCLVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2D278</accession>
    <name>F2D278_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123445733</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357985">
        <property type="protein sequence ID" value="BAJ89199.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978699.1">
        <property type="nucleotide sequence ID" value="XM_045122764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D278"/>
    <dbReference type="SMR" id="F2D278"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330040"/>
    </dbReference>
    <dbReference type="GeneID" id="123445733"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0330040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445733"/>
    <dbReference type="HOGENOM" id="CLU_046926_0_0_1"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22152">
        <property type="entry name" value="F-box_AtAFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF6">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ89199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="427" mass="48814" checksum="9AEF86FFC0CBB3B0" modified="2011-05-31" version="1">MQRRRLAEQEGESVSCYCRVDGGLKTVVNARKFVPGARLCMQPDVKPNKRKTRSSRKERCRTQAPLLPGLPDDLAISCLMRVSRAEHPNLRLVCRKWSRLLSGNYYYSLRKKFGMAEEWVYVFKRDRDQKLSWHAFDPVHQLWRSLPPVPPEYSEAVGFGCAVLSGCYLYLFGGKDPVRGSMRHVVFYNTRTNKWYRAPDMLRKRHLFGSCVINNCLYVAGGECEGIQRTLRSAEVYNPNRNRWSCISEMNTGMVPFTGVVYDGKWFLKGLDSHRQVVSEVYLPTSNTWSTTGNALVAGLRNPTIPFNGRLYSADCRDACKLRVYDGDIGLWTRFMDSRRHLGSSRAFEAVALVSLNGKICVIRNNMGMILVDVCDPTTVIEFESARLWETFARKGHQHRSLMANLWSAIAGRNLKAHIIHCQVLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5L5</accession>
    <name>F2D5L5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tricin synthase</fullName>
            <ecNumber evidence="7">2.1.1.175</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123410479</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>tricetin + 2 S-adenosyl-L-methionine = 3',5'-di-O-methyltricetin + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32347"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60016"/>
            <dbReference type="ChEBI" id="CHEBI:60045"/>
            <dbReference type="EC" id="2.1.1.175"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.175" evidence="7"/>
    <dbReference type="EMBL" id="AK359175">
        <property type="protein sequence ID" value="BAJ90386.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959361.1">
        <property type="nucleotide sequence ID" value="XM_045103426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5L5"/>
    <dbReference type="SMR" id="F2D5L5"/>
    <dbReference type="PaxDb" id="4513-MLOC_70730.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678040"/>
    </dbReference>
    <dbReference type="GeneID" id="123410479"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0562390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0562390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0562390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0678040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410479"/>
    <dbReference type="eggNOG" id="KOG1663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067676_5_0_1"/>
    <dbReference type="OrthoDB" id="10251242at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D5L5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000147">
        <property type="entry name" value="Caffeoyl-CoA O-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF66">
        <property type="entry name" value="TRICIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ90386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="247" mass="27102" checksum="10A6839575691CF3" modified="2011-05-31" version="1">MAPSGDNTVANVHSGIDSTNKTLLKSDALYTYILDTTVFPREHECMRDLRLITDKHPWGYMQSSSDEAQLLGMLIKMAGAKKTIEVGVFTGYSLLATALALPEDGKIVAIDTDRECYEVGRPFIEKAGMAHKVDFREGTGLERLEELLAEDDGAASYDFAFVDADKPNYVRYHEQLLRLVRVGGTIIYDNTLWGGTVALPAGTPLSDLDTRFSAALRDLNAKLAADPRIEVCQLAIADGVTICRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5M5</accession>
    <name>F2D5M5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440974</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK359185">
        <property type="protein sequence ID" value="BAJ90396.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362244">
        <property type="protein sequence ID" value="BAJ93448.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK368775">
        <property type="protein sequence ID" value="BAJ99978.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973446.1">
        <property type="nucleotide sequence ID" value="XM_045117511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5M5"/>
    <dbReference type="SMR" id="F2D5M5"/>
    <dbReference type="STRING" id="112509.F2D5M5"/>
    <dbReference type="PaxDb" id="4513-MLOC_65962.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321640"/>
    </dbReference>
    <dbReference type="GeneID" id="123440974"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440974"/>
    <dbReference type="eggNOG" id="ENOG502R3V8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MVCLEDK"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31208">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31208:SF3">
        <property type="entry name" value="OS01G0953500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="10"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ90396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ93448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="397" mass="42267" checksum="B434E25101CDACC7" modified="2011-05-31" version="1">MEPARFFRCVPGHGRLEEEDDEEQSSGGCFLDVYVHGARGIHNICIYADQDVYARFSLTSSPGHAPALSTRVAKGGGASPRFDERLPPLRVRRGRLGTDALKCEVWMRSCAESVLEHQLLGFALVPLADVAAADGARMPRREFSLSSTDLTHLPAGTVSLSLALRSGHGDACSVGPSECVVAEPSITSEVVILQPQAPPVDYLGIEFPDLNTARENEDMAVQYLPFLHLGMAPADAMETGTSPRGENSMPVSSDGSKNASTTTTTSDDRAIDVSSSPATKKPHHGAHEVTVSTPMCRASVDAPMSNGAAAEGKEKGGVFKSPMATCDIVDIEAEQSAMQRQIMEMYVKSMQQFTESLGAMKLPMELDGDGGAGVVVKPEAEAGKDGARVFYGSRAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F2D676</accession>
    <name>F2D676_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Laccase</fullName>
            <ecNumber evidence="5 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123430146</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Secreted</location>
            <location evidence="3 13">Extracellular space</location>
            <location evidence="3 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5 13"/>
    <dbReference type="EMBL" id="AK359386">
        <property type="protein sequence ID" value="BAJ90597.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969981.1">
        <property type="nucleotide sequence ID" value="XM_045114046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D676"/>
    <dbReference type="SMR" id="F2D676"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0158150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0158150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0158150"/>
    </dbReference>
    <dbReference type="GeneID" id="123430146"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0130300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0130300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0130300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0158150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0158150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0158150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430146"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF9">
        <property type="entry name" value="LACCASE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11 13" id="KW-0186">Copper</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043084256" description="Laccase" evidence="13">
        <location>
            <begin position="26"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="38"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="163"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="413"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ90597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0158150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="567" mass="61883" checksum="F000756062E3FA9F" modified="2011-05-31" version="1">MDREMFWLTYALVFLGCLGLPRADAAVVEHTFSVGNLTIDRLGQRQVITAVNGQFPGPMVEARDGDAVVVHVVNYSPYNITIHWHGVLQRLSGWADGPSMVSQCPIRPGGATYTYRFNVTGQEGTLWWHAHVSFLRATVYGALLVRPGPDRPRYPFPTPYGEATLLLGEWWNASVVDVERQAMLTGGPPNNSVALTINGMPSGYELAVRHGKTYLLRLVNAALSYQLFFKVAGHAFTVVAADACYTDPYDTDIVVLAPGQTVDALMHANASPGRYYMAAQVFQSVANATYTTTTTTTGLLRYEHGAAKAGGMSMIMMPSMPAFKDSATAQDFYGSLTGLLRDGKPTVPLHVDTRMLVTYGLGIAPCMPTQTLCNRTRGSVAASMNNVSFQLPKAMSLLEARMRGNVDGVYTRDFPDRPPVMFDFTNGSLSTNRSVMLTSKGTRVKRLRFNTTVQVVLQNTAILGSENHPLHLHGFNFYVLAQGAGNFNARTAVRSYNLVNPQQRNTVAVPAGGWAVIRFTADNPGVWVMHCHLDAHLPFGLAMAFEVDDGPTPDTVLPPPPPDYPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F2DER8</accession>
    <name>F2DER8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123449569</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362385">
        <property type="protein sequence ID" value="BAJ93589.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982775.1">
        <property type="nucleotide sequence ID" value="XM_045126840.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982776.1">
        <property type="nucleotide sequence ID" value="XM_045126841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DER8"/>
    <dbReference type="SMR" id="F2DER8"/>
    <dbReference type="PaxDb" id="4513-MLOC_27885.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0403760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0403760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0403760"/>
    </dbReference>
    <dbReference type="GeneID" id="123449569"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0403760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0403760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0403760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DER8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ93589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0403760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="10530" checksum="75A73F4C1957C8BA" modified="2011-05-31" version="1">MLQLTDLQRQGLGDEESGGTGFSPLPLFTDRSPPQSREEKDGGFKSPNGTGGDHAEPRLPRRARPAVVDRAPLLQDYKPHVQTKAGWLVGGPTLPSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2DGR4</accession>
    <name>F2DGR4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Arginine decarboxylase</fullName>
            <ecNumber evidence="5 14">4.1.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123413577</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amine and polyamine biosynthesis; agmatine biosynthesis; agmatine from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="5 14"/>
    <dbReference type="EMBL" id="AK363081">
        <property type="protein sequence ID" value="BAJ94285.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK376935">
        <property type="protein sequence ID" value="BAK08129.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962447.1">
        <property type="nucleotide sequence ID" value="XM_045106512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DGR4"/>
    <dbReference type="SMR" id="F2DGR4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644220"/>
    </dbReference>
    <dbReference type="GeneID" id="123413577"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0535160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0535160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0535160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0644220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413577"/>
    <dbReference type="OrthoDB" id="3717802at2759"/>
    <dbReference type="UniPathway" id="UPA00186">
        <property type="reaction ID" value="UER00284"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009446">
        <property type="term" value="P:putrescine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06830">
        <property type="entry name" value="PLPDE_III_ADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.930:FF:000003">
        <property type="entry name" value="Arginine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000001">
        <property type="entry name" value="Biosynthetic arginine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002985">
        <property type="entry name" value="Arg_decrbxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003763">
        <property type="entry name" value="PRK05354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01273">
        <property type="entry name" value="speA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43295">
        <property type="entry name" value="ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43295:SF1">
        <property type="entry name" value="ARGININE DECARBOXYLASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001336">
        <property type="entry name" value="Arg_decrbxlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01180">
        <property type="entry name" value="ARGDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 12 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0745">Spermidine biosynthesis</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="16">
        <location>
            <begin position="147"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="676"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="676"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="546"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001336-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ94285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="711" mass="75281" checksum="BE97EB601752AF11" modified="2011-05-31" version="1">MPALAVDAAAPVAHAFAACDASPRFAAPLLGPAAVVVAAAAAEKPEMAAWSADLSSALYNVDGWGAPYFFVNDDGDVAVRPHGAATLPGQEIDLAKVVARAAGARSGGGLGLALPLLVRFPDVLRHRVETLNAAFDYAVRSTGYAGRYQGVYPVKCNQDRYVVEDIVEFGAPFGFGLEAGSKPELLLTMSCLVARGSPDALLICNGYKDLEYVSLALIARTMGLNTVIVLEQEEELDIVVEASRRLNIRPVVGMRAKLRTKHAGHFGATSGEKGKFGLNAAQILSVVAKLKALGMLDCLQLLHFHIGSQIPTTALLADGVGEAAQIYCELARLGAAMRVIDVGGGLGIDYDGTHSAETDMSVAYSLEEYAAAVVAAVGRVCDRKGVQHPVICSESGRALVSHHSVLIFEAFSATAPASNMMDPATAYLLDELTDDCRNDYRNLMASAVRGDFDTCGLYADQLKRRCAEQFKEGVLGLEHLAAVDGLCEIVARGMGAAEGPRRYHINLSVFTSLPDMWAIEQLFPIIPIQRLQERPAVDGVLSDLTCDSDGKVDQFIGGRSSLPLHELPSHGTRGYYLGMFLGGAYQEALGGLHNLFGGPSVVRVSQSDGPHCFAVTRAAPGPSCADVLRAMQHEPEVMFEVLKQRTTDDGATAAALARAFGSMPYLSFDAEEAAAMSSAESSGMSSDSEGSAAGAAEDDDEEWEFMRGLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMI9</accession>
    <name>F2DMI9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123403293</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365107">
        <property type="protein sequence ID" value="BAJ96310.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953180.1">
        <property type="nucleotide sequence ID" value="XM_045097245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0604630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0604630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0604630"/>
    </dbReference>
    <dbReference type="GeneID" id="123403293"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0501250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0501250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0501250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0604630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0604630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0604630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403293"/>
    <dbReference type="OrthoDB" id="443832at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46922">
        <property type="entry name" value="DHHA1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46922:SF4">
        <property type="entry name" value="DHHA1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ96310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0604630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="344" mass="37672" checksum="3BDA4C1356C23364" modified="2011-05-31" version="1">MAGTARKASAVLYHYPCPDGAFAALAAHLYFSAAALPVRFFPNTVYDPIRSDGLPFDEIEDVYLLDFVGPPGFVADIAPKVQSVTILDHHKTAMESLCGSATLGENVNKVIDMQRSGATIAFDYFRNKLSTEASTSRNHGSGSSATEVKYLPENKLEMVHKLFKFIEDGDLWRWTIPNSKAFSSGLKDLDIEFNVNANGKLFDQLLELDPEQVISRGQVTLSKKQTLIDDCLEKSFEIALGCGQFGNCLAVNADAISMLRSELGNQLANKSRNSNLRAIGAVVYKVPELNNDEMLKISLRSLEQEDTTSISQEYGGGGHRNASSFLLSVAEFERWKVGAEPSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F2DNQ6</accession>
    <name>F2DNQ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123449296</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK365524">
        <property type="protein sequence ID" value="BAJ96727.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982389.1">
        <property type="nucleotide sequence ID" value="XM_045126454.1"/>
    </dbReference>
    <dbReference type="SMR" id="F2DNQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396140"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396140.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396140.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449296"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396140.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396140.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449296"/>
    <dbReference type="OrthoDB" id="784473at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010078">
        <property type="term" value="P:maintenance of root meristem identity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010492">
        <property type="term" value="P:maintenance of shoot apical meristem identity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010071">
        <property type="term" value="P:root meristem specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15612">
        <property type="entry name" value="PHD_OBE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047578">
        <property type="entry name" value="OBE1-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004082">
        <property type="entry name" value="OBERON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032535">
        <property type="entry name" value="Oberon_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21736:SF20">
        <property type="entry name" value="PROTEIN OBERON 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21736">
        <property type="entry name" value="VERNALIZATION-INSENSITIVE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16312">
        <property type="entry name" value="Oberon_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01544">
        <property type="entry name" value="ARATH130DUF"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="9">
        <location>
            <begin position="1048"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="domain" description="Oberon coiled-coil region" evidence="10">
        <location>
            <begin position="1277"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="889"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1302"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="290"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ96727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1401" mass="153268" checksum="EDB9DF28B10AE14A" modified="2011-05-31" version="1">MKRQRSSYVDDDDPDGDRRRFYDRGGPPSPPPPRRRPAEYESDEFDRRGGFGGRFYDHRYRDSPSPRGYGGDRAMHRSESFSGFRREFPKGFRADRDRSRWDASGSGSAWRRPGGPWRDPHGLDGHKSATRRQAPSPPTPPRRSPSEPRRRIDGPKGDKLRKHNCAAGEIEEGELAPDPDTKAPPPHPPATAEHQKWVDSGRADDKGTSKRCELKKVDSPGPRLRVDVRTQAADNLGKEKGKIRDDAVAEAGKATNTQHEKSALDVTGKVTNAQHEKSASDVTGKAANTQHEKSAASDVTEKVTNTQHEKSAASDVTEKVTNTQHEKSAPDVTEKVTNRQHEKSASDVTEKVCGGDEAASAIDQGGQSTSSVTRQDCVNAADVGGQSTSSGILKEVSMEEVTPQVETAIAVDDVGKGTSSSIQKESLQEAVMALGETASDDDIVWIGSSSTTLHKVLPKEATDGNINADGGVGNCISSSILQGAMQEEVKYVDVAADITDAPKEVSSFSMLKEAVHEDGTLLTANTVNLLGDSNSSVMLGEAMHETVTTKELTANVLDIAGKSSSSTMLQEDVMTSLCQESQEIKETEIGNVGDKKVDETTESIASQPVEGGLERYGCENRVALDKTEVVEEKEEAVENEIVAKQVKHIDLEANLVGAGVFLQSPKDHIKDTKGKGTTLDLIMEKPRAEDKGKGIAFDVLNKAGGGTLVGRGFDIGSQPDTDQKEAWKSTSTTSVKQEDDTLKIGRLDLSLSLSGGLQDPEFRSFIPRPDSVAHGPCSQLSSSSSSFCTNSDGITASVSLTNSQAFVRNPSFSHTQRSLDNCEHSVGSKPLFQGVDQVNNSTGWQVELSSNISTEKGNPTPLLQKVLQNGHLSDNTLVGVNMQNNGLSPVLSTAHNHGSLDAGLGHSRHRRQLTRERSSSSLSRGELQHEEQLVLNGAGVVERVISKIVSDPLNCTGRMLQEMTGNSRAYLREAISEIIINADKRGQIVALQEALKKRQDMNSEILQRCPRVLLEILVAIRTGLPDFVKKSNSIATPDLVDIFLNLKCRNLSCQSVLPVVDCDCKICKQKNGFCSSCMCIVCLKFDTASNTCSWVGCDVCLHWCHTDCGLRRSFIRKGGAGSRAHGTNEMQFHCGACGHPSEMYGFVKEVFRTCAKQWRVEALIRELQYVERIFSASDDVRGRRVRDFVKQMLIKLENRAYYSEVIKYVIAFFSDDNPNMGSGPLVPLKGIPCSTAEGINGIPSSSRTATWLPSVTLEGVPFLQKAGVVSTTGSQSMSRKIAETGFQAVNNKPVSDELDGLVRLKQAEANMYQERVNEARKEAESLKNIVMVKYARIEEHYATQMSELHINELQERRKQNIEELQVIERSYHQFLSMKTRMKDNIRELLLKMEATKQNLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQV9</accession>
    <name>F2DQV9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sucrose-phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123424708</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the final step of sucrose synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>sucrose 6(F)-phosphate + H2O = sucrose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="EC" id="3.1.3.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan biosynthesis; sucrose biosynthesis; sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the sucrose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.24" evidence="8"/>
    <dbReference type="EMBL" id="AK366277">
        <property type="protein sequence ID" value="BAJ97480.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964321.1">
        <property type="nucleotide sequence ID" value="XM_045108386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DQV9"/>
    <dbReference type="SMR" id="F2DQV9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0020190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0020190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0020190"/>
    </dbReference>
    <dbReference type="GeneID" id="123424708"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0015850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0015850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0015850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0020190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0020190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0020190"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030534_1_0_1"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="UniPathway" id="UPA00371">
        <property type="reaction ID" value="UER00546"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DQV9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050307">
        <property type="term" value="F:sucrose-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02605">
        <property type="entry name" value="HAD_SPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013679">
        <property type="entry name" value="SPP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051518">
        <property type="entry name" value="Sucrose_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012847">
        <property type="entry name" value="Sucrose_phosphatase_pln/cyn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01485">
        <property type="entry name" value="SPP_plant-cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521:SF2">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08472">
        <property type="entry name" value="S6PP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01141">
        <property type="entry name" value="C2.B.1:_Sucrose_Phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00043">
        <property type="entry name" value="sucrose-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose-phosphatase C-terminal" evidence="10">
        <location>
            <begin position="282"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ97480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0020190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="423" mass="47453" checksum="6D9D2F91AF471803" modified="2011-05-31" version="1">MDRLDGSARLMIVSDLDQTMVDHSDPESSAMLRFEALWESEYSQDSLLVFSTGRTPVSYKGLRKIKPLLTPDITIMSVGTVIAYGQEMTPDVGWEEFLNNKWDRDIVVQETATFPQLKPQPERNQGPHKVSFFVDKQDAQEVINSLPQKLEQLGLDVKIIYSSGEALDVLPQGAGKGQALIYLLEKFNLCGKPPNNVLVCGDSGNDADLFSVPSAYGVMVSNAQEELLEWYEENGKDNLKLLYATERCASGIMQAIGHFKLGPSFSARDLEFPYPKVDTVKPADVVVKFYVLYEKWRRGDVQKSPSVIQYLKSITHQNGTLIHPCGMERSLHASVDALSSCYGDKQGQKFRAWVDRLVTSPIGTSNWLVRFDNWEMEGDVQYCCRTTLLLNTKSGNPEGLELTHIHKTWLEGYLAGNAHTFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2DSR6</accession>
    <name>F2DSR6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123402531</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Secreted</location>
            <location evidence="2 14">Extracellular space</location>
            <location evidence="2 14">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the germin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK366934">
        <property type="protein sequence ID" value="BAJ98137.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952392.1">
        <property type="nucleotide sequence ID" value="XM_045096457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DSR6"/>
    <dbReference type="SMR" id="F2DSR6"/>
    <dbReference type="PaxDb" id="4513-MLOC_73808.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628410"/>
    </dbReference>
    <dbReference type="GeneID" id="123402531"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0521580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0521580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0521580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0628410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402531"/>
    <dbReference type="eggNOG" id="ENOG502QQ4A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015790_0_3_1"/>
    <dbReference type="OMA" id="LAHEATC"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DSR6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000280">
        <property type="term" value="P:regulation of root development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000025">
        <property type="entry name" value="germin-like protein subfamily 2 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 14" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090781" description="Germin-like protein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="15">
        <location>
            <begin position="61"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ98137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="22660" checksum="7EF6A88BC5ED9194" modified="2011-05-31" version="1">MARLQLSVLAACAILLALAAPSLAGDPDMLQDVCVADLASPIKINGFPCKANITADDFFFAGLKKAGNTNNPAGSVVTAANVQSFPGVNTLGVSMARIDYAVGGQNPPHTHPRATEIIFVTQGTLEVGFITTANKLFTRFVTVGEVFVFPRGLVHFQQNRGHGPASVIAAFNSQLQGTQAIATTLLAASPPVPTDVLAKAFRVDNEDIDAVKARFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXP6</accession>
    <name>F2DXP6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401518</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK368664">
        <property type="protein sequence ID" value="BAJ99867.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951277.1">
        <property type="nucleotide sequence ID" value="XM_045095342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXP6"/>
    <dbReference type="SMR" id="F2DXP6"/>
    <dbReference type="STRING" id="112509.F2DXP6"/>
    <dbReference type="PaxDb" id="4513-MLOC_58869.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0544390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0544390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0544390"/>
    </dbReference>
    <dbReference type="GeneID" id="123401518"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0452840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0452840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0452840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0544390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0544390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0544390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401518"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_6_5_1"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DXP6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000077">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000371">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF67">
        <property type="entry name" value="R2R3MYB-DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="10"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="10"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ99867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0544390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="313" mass="33804" checksum="069B86523B7D79F4" modified="2011-05-31" version="1">MGCRSCDKPKMNYRKGLWSPEEDQRLRDYILKHGLGCWSAVPAKAGLQRNGKSCRLRWINYLRPGLKRGMFSQEEEDVVINLQAKLGNKWSQIAMHLPGRTDNEVKNYWNSYLKKRVMQLGSNSSSNPASKNSASPELLTSMSANTELIMTSGGGGGSTATSTHDHDANIGSSGAVSAAEPFVDHQHHQHQDAKNYVFADWMPTAAAVTAAPGPESYSMSAHWPASTASSGNVTPSHGAFVGDQMSATSYGALQHTHQHHQQQEHQHHHQSAVTAVGAHGGAAGMVAGGGYFDLLNMGDIYGGFTTANDDLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZ51</accession>
    <name>F2DZ51_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398944</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYSTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369171">
        <property type="protein sequence ID" value="BAK00373.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371538">
        <property type="protein sequence ID" value="BAK02736.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949321.1">
        <property type="nucleotide sequence ID" value="XM_045093386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DZ51"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520880"/>
    </dbReference>
    <dbReference type="GeneID" id="123398944"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0520880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398944"/>
    <dbReference type="OMA" id="NEPKYAY"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DZ51">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028144">
        <property type="entry name" value="CYSTM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044850">
        <property type="entry name" value="WIH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31568:SF21">
        <property type="entry name" value="CYSTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31568">
        <property type="entry name" value="RCG49325, ISOFORM CRA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12734">
        <property type="entry name" value="CYSTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cysteine-rich transmembrane" evidence="6">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK00373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="8281" checksum="5382E9A7EF2939F4" modified="2011-05-31" version="1">MSDPKYAYPYPAQGYYQGGPYQGQGGPYQGPPVMAPPQYAAPPPPRAQPSFLEGCLAALCCCCLIDECCCDPSIIFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2E1T6</accession>
    <name>F2E1T6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123441205</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370107">
        <property type="protein sequence ID" value="BAK01308.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973641.1">
        <property type="nucleotide sequence ID" value="XM_045117706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E1T6"/>
    <dbReference type="SMR" id="F2E1T6"/>
    <dbReference type="FunCoup" id="F2E1T6">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2E1T6"/>
    <dbReference type="PaxDb" id="4513-MLOC_15416.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330210"/>
    </dbReference>
    <dbReference type="GeneID" id="123441205"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0275040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0275040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0275040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0330210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441205"/>
    <dbReference type="eggNOG" id="ENOG502QSMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CDEPDTH"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E1T6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF504">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090841" evidence="5">
        <location>
            <begin position="25"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK01308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="41236" checksum="E13B5D6BF14D1445" modified="2011-05-31" version="1">MERTSGPSWCILLCVAAAWMVAEAAPLPRYYNAIFSFGDSLSDTGNFVIINSGKLPNMPKFPPPYARCSNGRLVIDFLAEALGVPLLPPSANKGTNFSQGANFAVMGATALDLKYFKDNNVWSIPPFNTSMNCQLEWFHEVKETICSSPQECKDFFTKALFVFGELGGNDYSFAAKADWSTDKVKTKMVPKVVESIISGIEALLDEGARHVLVPSNLPVGCFPIMLTLFPFEDRSEYDPRTGCIKKFNGVALYHNARLRVALDQLQRRRPDSRIIYADFYTPYIQFARTPYLYGYKRGALRACCGGGGPYNYNMSASCGLPGATVCDDPDAHVSWDGIHLTEAPYRFIANTWLKGPYAHPPLASVVREDMAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F2E600</accession>
    <name>F2E600_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123401752</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371574">
        <property type="protein sequence ID" value="BAK02772.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951541.1">
        <property type="nucleotide sequence ID" value="XM_045095606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E600"/>
    <dbReference type="SMR" id="F2E600"/>
    <dbReference type="PaxDb" id="4513-MLOC_29841.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0593980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0593980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0593980"/>
    </dbReference>
    <dbReference type="GeneID" id="123401752"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0492200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0492200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0492200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0593980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0593980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0593980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401752"/>
    <dbReference type="eggNOG" id="KOG4824">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
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        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="OS02G0612900 PROTEIN"/>
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            <begin position="16"/>
            <end position="164"/>
        </location>
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        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK02772.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0593980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="190" mass="21762" checksum="B127C43E62631E85" modified="2011-05-31" version="1">MAVKKIGSEMGVVLGLDVARYMGRWYEIASFPNFFQPRDGRDTRATYELMEDGATVHVLNETWSKGKRDYIEGTAYKADPASDEAKLKVKFYVPPFLPIIPVVGDYWVLYVDDDYQYALVGEPRRKSLWILCRKTHIEEEVYNQLLEKAKEEGYDVAKLHKTPQSDPPPEGDAAPTDSKGAWWFKSLFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
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    <name>F2E8D3_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC123422116</name>
    </gene>
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        <lineage>
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            <taxon>Poales</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Seed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017880.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC ION BINDING PROTEIN"/>
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    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="795"/>
        </location>
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        <source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="BAK03605.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2ED33</accession>
    <name>F2ED33_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COBRA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429721</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Flower</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the COBRA family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374058">
        <property type="protein sequence ID" value="BAK05255.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969663.1">
        <property type="nucleotide sequence ID" value="XM_045113728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ED33"/>
    <dbReference type="PaxDb" id="4513-MLOC_75040.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127260"/>
    </dbReference>
    <dbReference type="GeneID" id="123429721"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0105030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0105030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0105030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0127260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429721"/>
    <dbReference type="eggNOG" id="ENOG502QQYT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2012261at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2ED33">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052324">
        <property type="term" value="P:plant-type cell wall cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673:SF23">
        <property type="entry name" value="COBRA-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038122">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090917" description="COBRA-like protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="6">
        <location>
            <begin position="219"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK05255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="444" mass="48929" checksum="8D692D6227F2B4FC" modified="2011-05-31" version="1">MELPNAALVLILAAAATLSVAVAYDPLDPNGNITIKWDVLSWTPDGYVATVTINNFQTYRQIMAPGWTVGWTWAKREVIWSMVGAQATEQGDCSKFKANLPHSCKRTPAVVDLLPGVPYNQQIANCCRGGVVSAYGQDPSGAVSSFQVSVGQAGTTNRTVKVPKNFTLLGPGPGYTCGPAKVVPSTVFLTADHRRKTQALMTWNVTCTYSQHLASKYPTCCVSFSSFYNDTIVPCAKCTCGCEHKTCARSERDSKRLMSASGKSAVTARGHVNRQSAAPLLQCTTHMCPVRVHWHVKLNYHDYWRAKVTVTNFNYRMNYTGWTLVAQHPNLDNITEVFSFDYKPVVSYGSINDTALFYGMKFFNDQLMEAGPYGNVQSEVLMRKDASTFTFRQGWAFPRKIYFNGDECRMPPPDSYPYLPNSAPASLLSSASAVIVAFLVLLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F2EE29</accession>
    <name>F2EE29_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123443218</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Flower</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374405">
        <property type="protein sequence ID" value="BAK05601.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975461.1">
        <property type="nucleotide sequence ID" value="XM_045119526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EE29"/>
    <dbReference type="SMR" id="F2EE29"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247870"/>
    </dbReference>
    <dbReference type="GeneID" id="123443218"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0205850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0205850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0205850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0247870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443218"/>
    <dbReference type="OrthoDB" id="193856at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000115">
        <property type="entry name" value="Mitochondrial arginine transporter BAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF10">
        <property type="entry name" value="SLC (SOLUTE CARRIER) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="10"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="117"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="214"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAK05601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="35359" checksum="17D61FC25FDFD609" modified="2011-05-31" version="1">MEFWPEFLATSGGNEFVAGGVGGMAGVLAGHPLDTLRIRLQQPPRPMSPGITAARVARPPSAVALLRGILRAEGPAALYRGMGAPLASVAFQNAMVFQVYAILSRSLDRRMSTSEPPSYTSVALAGVGTGALQTLILSPVELIKIRLQLEEAGQKHRGPVDMARDIMRREGMHGIYRGLTVTALRDAPSHGVYFWTYEYARERLHPGCRRTGQESLATMLVSGGLAGVASWVCCYPLDVVKSRLQAQTQTHPPSPRYRGVADCFRKSVREEGFPVLWRGLGTAVARAFVVNGAIFSAYELALRFLVRNNGRQTLVMEEMKCHDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFC9</accession>
    <name>F2EFC9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430918</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Seed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK374855">
        <property type="protein sequence ID" value="BAK06051.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970682.1">
        <property type="nucleotide sequence ID" value="XM_045114747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFC9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0199860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0199860"/>
    </dbReference>
    <dbReference type="GeneID" id="123430918"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0166020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0166020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0166020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0199860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0199860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0199860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430918"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043303118" evidence="1">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK06051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="88" mass="9137" checksum="5A1180FC28E300E2" modified="2011-05-31" version="1">MAGTKAAVMKLAVLLLAMSQLVHMETPTADPTTASSLLVTSVSAPEVEVHPTPSHGIREEKVLLDAAKPGIPVPPPPHLILPCKGRAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>M0WZG8</accession>
    <name>M0WZG8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine dioxygenase</fullName>
            <ecNumber evidence="3">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044948474.1">
        <property type="nucleotide sequence ID" value="XM_045092539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WZG8"/>
    <dbReference type="SMR" id="M0WZG8"/>
    <dbReference type="FunCoup" id="M0WZG8">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WZG8"/>
    <dbReference type="PaxDb" id="4513-MLOC_55837.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0473030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0473030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0473030"/>
    </dbReference>
    <dbReference type="GeneID" id="123398026"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0391810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0391810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0391810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0473030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0473030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0473030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398026"/>
    <dbReference type="eggNOG" id="KOG4281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WZG8"/>
    <dbReference type="OMA" id="VWLEEHQ"/>
    <dbReference type="OrthoDB" id="271433at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WZG8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070483">
        <property type="term" value="P:detection of hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20289">
        <property type="entry name" value="cupin_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012864">
        <property type="entry name" value="PCO/ADO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966:SF61">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07847">
        <property type="entry name" value="PCO_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0473030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="238" mass="26812" checksum="86799E30D25FFF48" modified="2013-04-03" version="1">MAAIQKLYEVCKVSLFEKGPSSPEAVENVRAVLDMITPSDVGLECEAQAVRVWRRPRALSRKTVLHSSPAIRYRHIYECKSFSIGIYCIPASSIIPLHNHPGMTVLSKLLYGKVHVKAYDWIDIDEPGNLSKVRPAKVVRHGEISAPCAAMVLRPTDGGNVHALKAITPCAILDILSPPYSSKDGRHCSYFRRRPKSDQSGILWDRTRESEFVLLEEYQPRDNFVIRRDLYTGPTLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>M0XV04</accession>
    <name>M0XV04_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123397971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948414.1">
        <property type="nucleotide sequence ID" value="XM_045092479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XV04"/>
    <dbReference type="SMR" id="M0XV04"/>
    <dbReference type="PaxDb" id="4513-MLOC_63270.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504890"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504890.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504890.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504890"/>
    </dbReference>
    <dbReference type="GeneID" id="123397971"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0419350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0419350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0419350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0504890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0504890.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504890.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397971"/>
    <dbReference type="eggNOG" id="KOG2536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CARCNPL"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XV04">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF38">
        <property type="entry name" value="OS09G0557400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="26916" checksum="6CE77B2C70EDFB00" modified="2013-04-03" version="1">MARALLRRRGALSSLLSPAAPTAPRLVPSFSSATATATATAVAPLRSTLDERLLRLLRSEISYAADRRPPHQPPTGFKSFAVEDRPGEQWVRLRAARRGAGSRTEEEAIKIDATLFDGVAETPPDASLFHRVEALEQGPRLHLSLIVEVARADRVLGFICSAWPDQLTVRHVLTLSGAGAATDDRGAREFTKLEPAEREAVKKFLLEREVDAELAEFLHDYVANKEKMELLRWLKTVESFVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0YJT8</accession>
    <name>M0YJT8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123427963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
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    <dbReference type="eggNOG" id="ENOG502S8M9">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
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        <property type="entry name" value="S1_domain"/>
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        <property type="entry name" value="TIF_IF1"/>
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    <keyword evidence="15" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 11 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 15" id="KW-0934">Plastid</keyword>
    <keyword evidence="9 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="14">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003935"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004229"/>
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            <dbReference type="ARBA" id="ARBA00022640"/>
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            <dbReference type="ARBA" id="ARBA00068272"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="AGP50789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AMA20280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QGL07966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0754180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="13116" checksum="6D2577079295F0E8" modified="2013-10-16" version="1">MTEKKNRREKKNPREAKVTFEGLVTEALPNGMFRVRLENDTIILGYISGKIRSSSIRILMGDRVKIEVSRYDSSKGRIIYRLPHKDSKRIEDSKDSEDLKDSEDLKNTKDSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287H0V5</accession>
    <name>A0A287H0V5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123424475</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="18"/>
    <dbReference type="RefSeq" id="XP_044964029.1">
        <property type="nucleotide sequence ID" value="XM_045108094.1"/>
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    <dbReference type="SMR" id="A0A287H0V5"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102890"/>
    </dbReference>
    <dbReference type="GeneID" id="123424475"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0084600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0084600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0102890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102890"/>
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    <dbReference type="KEGG" id="hvg:123424475"/>
    <dbReference type="OrthoDB" id="1930390at2759"/>
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        <property type="component" value="Chromosome 2H"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000237">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000013">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF89">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE LECRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="819" mass="91238" checksum="7F48D48D4A2DAF19" modified="2017-11-22" version="1">MAPRLPWCFLQFLLLLLGVSSAAQAQVNITRGSSLTPQGPTSSWLSPSGDFAFGFRQIEGNSSLYLLAVWFNRIHGETVVWYAKSSSDGQESPVQVPSSSVLRLTADGLLSLRNPSGDEVWSPPVPAVAYARMLDTGNFMLVGVDGKAKWESFDFPADTILPTQVLSVGQQDKVLRSRLIATDYSNGRFLLAVQTDGNLVFYPIAEHSTLRYNAYWASNTVGNGSQLVFNETGRVYFTLINGTQINITSAEGYSMDTQGDFFHRATLDPDGVFRQYLYPKSRKMRGQGTSQWTSVSSIPQNICQRVKVKAGSGACGFNSYCTIDGTKNQTSCQCPQRYEFFDEERQYKGCRPDFVPQSCDLDEEAARAQFEMTLVNSVDWPESDYEQYSPIDVYECQRLCVIDCFCATAVFQPSTNTCWKKKLPLSNGYMAESVDRTVLIKVPRGNNSQSQNTGSSKWKKDKKYWILGSSLFFGGSVLVNILLISILLFGTYCGISITSKKKLQSPQPSGSSILPPKIFTYNDLEKATSGFREVLGSGASGTVYKGQLQDEHATSIAVKKIEKLQQETEKEFMVEVQTIGQTFHKNLVRLLGLCSEGTHRLLVYEFMTNGSLNEFLFGDARPDWNLRVQVALGVARGLLYLHEECSTQIIHCDIKPQNILLDANFMAKIADFGLAKLLRANQTQTNTGIRGTRGYVAPEWFKNIGITSKVDVYSFGVILLELVCCRRNVELEIADEEQAILTYWANDCYRCGRIDLLVEGDDEANFNIKKAERFVAVALWCLQEEPTMRPTMLKVTQMLDGAVQIPTPPDPSSFISSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287ND00</accession>
    <name>A0A287ND00_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transport inhibitor response 1-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287ND00"/>
    <dbReference type="SMR" id="A0A287ND00"/>
    <dbReference type="STRING" id="112509.A0A287ND00"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0344800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0344800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0344800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0287800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0287800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0287800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0344800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0344800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0344800"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287ND00"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF36">
        <property type="entry name" value="TRANSPORT INHIBITOR RESPONSE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0344800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="36776" checksum="A8C1554D45C3E7DC" modified="2017-11-22" version="1">MARAPHLTHLGTGSFRSEPGPGGALSVSELATCFAASRSLVCLSGFLDVNGAYLPAIYQVCPNLTSLNFSFAALTDEEFIPVIRHCINLRTLWVPDTVGDEGLRAVAETCSNLRELRVFPLDATEDSEGSVSDIGLEAISEGCRKLESILYFCQRMTNAALVAMSENCPDLLVFRLCIMGRHRPDRITGEAMDEGFGAIVMNCKKLTRLLVSGLLTDKAFAHIGKHGKLIKTLFVAFVGNSDMPLKHVFEVRDSPFGDKGLLSGLNYFYNMRFFWMNSCRLTVRGCGDVAQQMPNLVVEVMKENEGEINTIDKLYLYRSLAGPREDAPSFVNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287XV96</accession>
    <name>A0A287XV96_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123413581</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the polygalacturonase-inhibiting protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962449.1">
        <property type="nucleotide sequence ID" value="XM_045106514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287XV96"/>
    <dbReference type="SMR" id="A0A287XV96"/>
    <dbReference type="PaxDb" id="4513-MLOC_27185.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0743100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0743100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0743100"/>
    </dbReference>
    <dbReference type="GeneID" id="123413581"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0616110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0616110.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0616110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0743100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0743100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0743100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413581"/>
    <dbReference type="OrthoDB" id="785757at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287XV96">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051848">
        <property type="entry name" value="PGIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48059:SF30">
        <property type="entry name" value="OS06G0587000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48059">
        <property type="entry name" value="POLYGALACTURONASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="11">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0743100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="285" mass="31216" checksum="82AF728296BA190F" modified="2017-11-22" version="1">MATRRSLEPSTLVLPALLLLLFYGAGNAYCSVVSGNTTDRRSLLDFKKAITKDPTGALRSWNDSINYCMWLGVNCSRTHPGRVTVLNLENLSLEGEITPSIGSLTFLKYLDLSNNSLQGNIPDALANCSRLTILDLSSNSLQWNIPGSLSNLGVNIMDFSNNNLTGNIPPTFSNMTQLLAISLAHNHLKGSIPEELGLLPNMVWVILRENRLSGRVPAKLFNLSYLLVLDVRQNMLSGTLPSDIGNLLPSLQVLYLGTNKFEGHIPDSLANASLLQRLDLSSNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WBJ8</accession>
    <name>A0A8I6WBJ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WBJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0130480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130480"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035257381" evidence="1">
        <location>
            <begin position="21"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="201" mass="21460" checksum="285476EFE36CC93E" modified="2022-05-25" version="1">MMAPTRSMLLLLTYAPLAAAMLQQPSEARVQAVKAPSVPHLAGDQEAEAVAATSSPASPFNGVLVFMPPLPPLPTWFARVTAVARLLRHGRRGRAIVTFAASTAGQLLGGAGACGNLRALPHGLQRTRAAWNVLRRLPIARCSSARICLSHAIMGNLSTFAGGTIDQLRLFVLPLTCNTFVLPELTLKCFLDQVPPIMPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WEY3</accession>
    <name>A0A8I6WEY3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WEY3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0012980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0012980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0012980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0016820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545:SF13">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="2">
        <location>
            <begin position="133"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="418" mass="46647" checksum="C172482179A980DC" modified="2022-05-25" version="1">MRAMVCSVRSLRAGRHRRVCGKLSLEFFAFSTSATINRLVVDAVDSWGVEDLEVVAKSTEPITHVRPVYKFPRGRISRKPGESRLRSLKLVNCLSPPLEGFTALTTLLLRDLPCSTPAAVYEGVVTACPQLRVLHLISCSFDRDTAGWLVFDAPMSEIRELVADGPFMTVKLRSLPKLERLAVVDARVLLCSDANVPCLAHVSLVFSIGPLDNHSIVNHLISMFMLSLKDTASSMKNLILRFTGPEMWIAPNLKNQFSLMPNLKKLLVADVPSSWDVSWPRVLIQAAPLLESLHVHVSQSQCQHDEEPDRQNVSSYLQQPSTSSSSGQRHRHLKELVVVGFQSMSRIHHQVIYLVRFAVDTSTALRRVAVFKHGHVEDKGGPWDWEMVSKQSTWSNDEKLAVLDCCVTSTPQIEVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WHC4</accession>
    <name>A0A8I6WHC4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123429603</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044969563.1">
        <property type="nucleotide sequence ID" value="XM_045113628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WHC4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0119930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0119930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0119930"/>
    </dbReference>
    <dbReference type="GeneID" id="123429603"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0098720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0098720.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0098720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0119930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0119930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0119930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429603"/>
    <dbReference type="OrthoDB" id="601557at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="208"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="286"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0119930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="38642" checksum="51EE072D2E191299" modified="2022-05-25" version="1">MARKKVPLRYIGNGAKETSRRRAYETRRKNLMKKAREMGILCNTKACVVVYDEGASAPDVYPSHAEAVAILNRYKDTPNMPRFKKVVTQEEVLTQRVAKLRHEADKLRREREDRETRMLLHKAMRGGNLNVEEVARVGSKLEEILKSLGERIAKNSMQPPVFQPQEPYGTGSVDIPAYQVPLPQQQQGPQPPVFQPQAPYVTGNVDMGPSPTYQMPPPPQQQQQGWQPPVFQPQAPHVTGSADMGPLPTYQVQPQQHHQGWKPSDLLPQEPYVTGNVAMGAPRMYQAPPPQQEGWLDTGRSEGNHDALLYNANSTGAPDGTATSSSAGFPFDDLMQFFDDMGSEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WKX5</accession>
    <name>A0A8I6WKX5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444413</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044977052.1">
        <property type="nucleotide sequence ID" value="XM_045121117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKX5"/>
    <dbReference type="SMR" id="A0A8I6WKX5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070230"/>
    </dbReference>
    <dbReference type="GeneID" id="123444413"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0070230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444413"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031099">
        <property type="entry name" value="BRCA1-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763">
        <property type="entry name" value="BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN BRCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763:SF8">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="162"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="10">
        <location>
            <begin position="462"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="10">
        <location>
            <begin position="581"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="91"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="699" mass="77303" checksum="119D71E31B47020F" modified="2022-05-25" version="1">MVSMGTSTGSRSGAVVWREIASSQPPGFGRPTGSVGGTASRSERPSPAQGLAAARPSSTHSGGTKSATRTDMLVGIGMEADCDNLEVPRHVGNERASPAKGRKRKFFIDTTKGASPGVNASNTGQHLRSCRKGKGPGKHAKLEDNKGATRLGRDQTPIGLFEDECVFCHSFRTSEPWHGPMVVYHNRRIVPSDEGIPTNAVYVHERCMVWAPEVESNGDTFKNVENEINRAKRLRCSRCKLRGAALGCYDNSCRKTYHVPCAMMILECRWDVENFRVWCPKHAARDEKRPPTMESDTPSLVLQNHSSQCPAKEISVDCQMEDEHINPLPGDEMSSPLLESDTTSAVLQNHSSQCPTKEILVECQVEDEHINPLPSGEVSSLTMESDTLSPVLRNQYLVKGGTSVLHRREDLQVDQLNTSSSSLPQGVFHGYRQCSDKERISANYRREEKLVNQSSTPVDQWVLLGVSLSVSEKDSLKEFASLTSSILAEAWDRNVTHVIVGRNAGDACGKSYEVLMAILSGKWIVTVRWIMDCLVEPIPDVKTCLEKLVPGPEISYEVNFCDGSRTSGYGPTKGRARAAERAPKLFSGLHFCLSAYMDPEDRETIRRLVAAGGGQVLEGISPDRLHENLNRNPAEVYFIYDSSPPRKITKDFDLILGKEIQESIEYAKSGAHVISHTKLHDAILYYDARILERRLHEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMV0</accession>
    <name>A0A8I6WMV0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123402302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952142.1">
        <property type="nucleotide sequence ID" value="XM_045096207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMV0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088890"/>
    </dbReference>
    <dbReference type="GeneID" id="123402302"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0073110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0073110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0073110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0088890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402302"/>
    <dbReference type="OrthoDB" id="787182at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34680">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34680:SF8">
        <property type="entry name" value="WRC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51667">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WRC" evidence="4">
        <location>
            <begin position="177"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="361" mass="39515" checksum="6B3D2DA41FE5F577" modified="2022-05-25" version="1">MRIRRSAARLLGSAYPAPAPPQSEHPPPPPPVRQPCSSRAGGGGFTAGGASSAEAERCELSRSPWELMAQLDPSDPKEVELFMGRYFVSVACRTNWLFQASIDLAASIKGEEQDDWKEEHLAGDMVAEVFAKRQHKVAKKKVAEKTVMENRGDDKFVWGLKKSTEIQSEDDDFVAAGGELWLCKKNDGKRRFCRRPVSQPDSLCVYHSDPKYAPPPASSAASKLSSSSKPRKRRRVDAGEGYLYYAGFGPSLGKRQRSSSSVLESVPAEQKEEAQLPEEHAAAPAQTDDADNQAASAHVDEPRCDEMAGIAGGDEESSDDALGCNDEPRVVGVKGNIKRKSPFKKRWRKPVKARSLKSLMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNK4</accession>
    <name>A0A8I6WNK4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FRIGIDA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123424665</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Frigida family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964257.1">
        <property type="nucleotide sequence ID" value="XM_045108322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNK4"/>
    <dbReference type="SMR" id="A0A8I6WNK4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110710"/>
    </dbReference>
    <dbReference type="GeneID" id="123424665"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0090770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0090770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0090770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424665"/>
    <dbReference type="OrthoDB" id="1166059at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012474">
        <property type="entry name" value="Frigida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791:SF9">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07899">
        <property type="entry name" value="Frigida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="3 4" id="KW-0287">Flowering</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="539"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="609"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="563"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="650" mass="70939" checksum="8BEE4DFE4BD89757" modified="2022-05-25" version="1">MASELEAAIAALPAKKQRLRDSFDRLAAASPVPIPFTWADVDAHISSLQSSIAARFRLLQSRQAAALPATADTVRRHVRGKGQDSSEEEEDGGRAKRAPLDRNAGEVGGQASAEMPVESSRGLYGAVEEGEASPGLEPEEEEDGARDNRPPSDLNAGEEGEQGRAETAPEWSPDQDDAVEEGEQGRADMAPEWSPDQDDAVEEGEVSPGLEPEEEEEEDEEDAEETTTGAYPGHRHGETTPEPAGGAGEARRWDLAAARAVRDASVLADMLYWGNKRSLRARSQFLPALRCVAEPHALVVGAVRDFLDRTEPKSDKNWENCAALLCLVPNLAAEPSAATLDRAGRLAADWREMIVGKPEGRRDLGRLAVWGLLQFLVSYKITLELDARGTMHLLADVPRNKKQSCVELCNSLGMVQTVADSVNYLVESGQQRAAINYLIENGQQLDAIKLACDLNLTDRYPPLTLMNEYVEKAKKTALEILSREGDSPESLNEAMTEQVNALILSWKAVDEHVDMADRTGVKAEITQLLHGYAHKRLSLPGASSSSSSPAWSPQQQQPPPTPEWRRQQKERHGGQRPGEKQSRKRTREHDYHRNRKCITRAERWRSRFAELSPGGGAGGSFACDAGGIGDRRGQHFPEDQGGPSPCSGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WRS4</accession>
    <name>A0A8I6WRS4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WRS4"/>
    <dbReference type="SMR" id="A0A8I6WRS4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0026220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0026220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0026220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0020790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0020790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0020790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0026220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0026220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0026220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF37">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GP-PDE" evidence="6">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0026220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="166" mass="19394" checksum="92CEFB1A311375B7" modified="2022-05-25" version="1">MSKDWLKKPLFIQYFAPTSLIYISNMTNAPKLLLIYPTTVPTEDTNQSYYEITSNGCLTFIRKYVIGIGPWKDTIIPPNNNHLGLATDLVARAHALNLQVHPYTFRNENSFLHFNFHQDPYAEYEYWLREIGVDALFTDFTSSLHKYQEWTAPRQRKEKKCRGTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WS33</accession>
    <name>A0A8I6WS33_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123443880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976357.1">
        <property type="nucleotide sequence ID" value="XM_045120422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WS33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0271640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0271640.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123443880"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.3HG0225140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0271640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0271640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0271640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443880"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14011">
        <property type="entry name" value="PK_SCY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="734"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="900"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="734"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="909"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="930"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="949"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0271640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="968" mass="104307" checksum="141B3036A6B29BD0" modified="2022-05-25" version="1">MSLNMKTLQQALAKASAVIEKTVTTTVQEVTGPRPLQDYELLDQAGSGGPGLAWRIYTARPRDAAASTPYPVVSVWVLDKRALSEARARAGLSRAAEDAFLDLARADAARLVRLRHPGVLHVVQALDENKAAMAMVTEPLFASVSNALGCLDNVGKVPKELKGMEMGILEIKHGLLQVAETLDFLHNNAHLAHRAISPETVFITSSGSWKLGGFGFALSVDQATGGLASSQQFHYSDYDVEDTALPLQPSLNYTAPELVRSGDSKVGSTCDIFSFGCLAYHLVAHRPLLDCHNNVKMYMNSLTYLTSEAFSNIPTDLVSDLQKMLSVDAASRPSAMAFTGSSFFRHDTRLRALRFLDHLLERDNMQKTEFLKALSDMWKDFDSRVLRYKVLPPLCAELRNMVMQQMILPMVLTIAESQDKDDFELSTLPALVPVFTSASGETLLLLVKHADLIINKATNEHLISHILPMLVRAYDDTDPRLQEEVLRRTVPLSRQLDMKLLKQSVLPRVHGLALKTTVAAVRVNALRCLGDLVPSLDKTGIVEILQTLRRCTAVDHSAPTLMCTLGVANAIYKQCGVEFAAEHVVPLVFPLLTAQQLNVQQFAKYILFVKDITSKIEEKRGVTVTDNGHTEVKVSPSISNGIHFKPTSGGMGQTGQMPAAKSTSWDEDWGPTKKTSAPTLSFDSSVQTKQPSKDPFDFSTQTNQPLTLPFDLSTQMKQPSTVTQVAASTIPSAQPLPSLQSLAPSSGHQTSGSCVPVDIEWPPRMSTSSDFNAPFSANKDSKSGELSNNGVDDVDPFADWPPKASSAASISAVERLPSTNQSISGLNTGNMGFGGSSTSLGQMKTNQVSWSAKPNNTNIMGMNSTVSYLNQGNSSLGYGNPIGGPSAGLSNAASPIAGQSIRQPKSDFGSLSQLSSGTQGPPRLAPPPSSAAVGRGRGRNQGQSGLSRASRTPNSNVSSEQPPFLDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUW6</accession>
    <name>A0A8I6WUW6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123412949</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961843.1">
        <property type="nucleotide sequence ID" value="XM_045105908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066310"/>
    </dbReference>
    <dbReference type="GeneID" id="123412949"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0052970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0052970"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412949"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF202">
        <property type="entry name" value="OS05G0413000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035315576" evidence="4">
        <location>
            <begin position="23"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="377" mass="41657" checksum="50B78E4D8428E9DB" modified="2022-05-25" version="1">MLPAARRRLCLLPLPLRHFTAAVYPTTSATADPTVFYLQSTCALSPTAAARAADSIRLASPESTAQASAVLDLLRRYGFTDAHISATVRKFPIVLVSDPVKTLQPKLDFLASVGINTPLLPRLVSLSPIVLHRSIQDHLAPLFESLRELLGSNARVVTALHHMPFVVRCSPNSTLNLVLPVLRDVHGLPPEDVSKLVAVHPGVIMQAPHRLAEIVQAVKDAGIEPGEPMFVHTFAILSKMKTHTLERKYALYQSLGFQKDSVALMLRRYALAMAISEDKIKENVGFLVGRAGLSLEDIVTYPSMLVRSLESHCRRCAVLAVLRKEEKPEGNHRLAVVLVTTRKRFLQAYVQPYQNEIPDVFRAFNGEIPFEGFSVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WX64</accession>
    <name>A0A8I6WX64_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WX64"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0209400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0209400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0209400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0174150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0174150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0174150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0209400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0209400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0209400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46934">
        <property type="entry name" value="MYB_DNA-BIND_3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46934:SF12">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0209400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="171" mass="19885" checksum="0EB8C83B143D4754" modified="2022-05-25" version="1">MMTARGSPRLNLLKEVAERHRASPPTGSVEKRRRGSPKEDRAQWNASLEKDLVDLLREHATPEHKGQNGWSSEAWNKIVKKFHQKNPYARYEKKKIQEKEKELKRYYRMIKEIRKQSGVSWDDQQCKILADPPLWKNIIISHPKAGKFKTKGFPLFDALGELHDGQTVEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXL5</accession>
    <name>A0A8I6WXL5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123443018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044975168.1">
        <property type="nucleotide sequence ID" value="XM_045119233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXL5"/>
    <dbReference type="SMR" id="A0A8I6WXL5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0240570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0240570"/>
    </dbReference>
    <dbReference type="GeneID" id="123443018"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0199670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0199670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0199670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0240570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0240570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0240570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443018"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000104">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF225">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="5">
        <location>
            <begin position="19"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="496" mass="54862" checksum="1AA5F588B04B4E72" modified="2022-05-25" version="1">MTFTSSGDGQSGSMRAHFVLVPMLAQGHTIPMTDMARLLAEHGAQVSFITTPVNAARLAGFAADVEAAGLAVRLVELYFPSTEFGLPDGCENLDMIHSTDLLSNFMDAIAALQEPLKAYLREQQCSPPSCVISDLIHWWTGDIARELRIPRLTFSGFCGFSSLIRYITFHNNVFENVKDENELITITGFPTPLEITKAKCPGGFSIPGMEQTRKKFLEEELKSDGEVTNSYQELEALYIESFEQTTKKKAWAIGPMCLCHRDNNTMATRGNKASMDEAQYLQWLDSMKPGSVVFVSFGSLACTTPQQLVELGLGLEASRKPFIWVIKAGAKLPEVEEWLADGFEERVKNRGMVIRGWAPQLMILRHQAVGGFVTHCGWNSTIEAICAGVPMITWPHFGEQFLNEKLVVDVLRIGVEVGVKGVTWWGNEKQEIVVTRDEVEKAVSTLMDEGAAAEETRVRAKDCAIKARKAFDEGGSSYDNIRLLIQEMGSRTNACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYB0</accession>
    <name>A0A8I6WYB0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123451067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044984018.1">
        <property type="nucleotide sequence ID" value="XM_045128083.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WYB0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090370"/>
    </dbReference>
    <dbReference type="GeneID" id="123451067"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451067"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000090">
        <property type="entry name" value="E3 ubiquitin-protein ligase RFWD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044630">
        <property type="entry name" value="SPA1/2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218">
        <property type="entry name" value="PROTEIN SPA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218:SF6">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF PHYA-105 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1085"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1110"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1196"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="223"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="899"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="851"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="223"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="375"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="910"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1335" mass="148820" checksum="39E99227C8DCE6F0" modified="2022-05-25" version="1">MEGARAAELAGDVQIKGGQAAQPLPQDQQQQLPTGSEAVEMPATPLPHAREIEWSEHFSFFNSMATATGTGEPASVGMSRSESSRPDSVTQQQQQQQQRCLEDERVEELTVRNCISSEAQQPAASAGGSTSSSGERPAVMRGLWGNFTRMAWRTADAASREALAVSRGDIANMRNGDLVGRENLAVSLGNGMGSQNIDASAKEMPFGRGENGNAEFNMSFGNQQQQHILSSRPNQSEQHILSSRPNQSEQHILSPRPNQSEQHILSSRPNQSEQHILPSRQNQSEQHVMSSWLNQSELHIPSSRPNQSEQHILPSRQNQSEQHVMSSWPNQSELHIPSSRPNHNGQHVLSSRPDQSEKHVLSSRPDQSEKHVLSSRPNQNEQHVLSSRPNQSEQHVLSSRPNQSEQHVLSSRPNQSEQHILSSRPNQSEQHILSSRPNQSEQHILSSRPNQNEQRSERENGPKVSSFSNRIIDQMRSKTLPPLSGVQGSPFKPVLKGKRVTYQGSHEEIQVQANARPRTPMDKIPKIPSSTHDSVARLDGTLFSSGGNVSESQYEGTSLRELIKPACQAMSKFEKMHLFKQILEHVDKSHAQGVTLQHLRPSYFIISSPNQVRYTGSYTKQDLSTPAKPDMAADDVFNRKRCFDQKTLHQECNGNGHSILKYRKVGEQGSVAVRRPIHPFWTDHKVGNQSDGADLGALGQKFGEPYYGGNASYGQRLPSYGNQESVLELRMLEDSWYRSPEELNQLKGTFPANIYSLGVLLFELFSCCETWELHCAAMSDLRHRILPPSFLSESPKEAGFCLWLLHPDPFSRPKARDILGCDLINEGRDLSLLDKAPAAISEEDTESGLLLNFLSQLKEEKETQAAKLSADLSSLQTDIVEAERRHSLRMGFSLEDMDVPASSNDVPGTSSNALRGASLSGLLPPSGRSDIYEERVMKNLQQLENAYYSTRSTIDTSETNVIKRSDNDALRVRENFNHSDDDAMAVPTDRLGCFFDGLCKYARHSRFEVRGILKNADILNSPNVICSLSFDRDEEYFAAAGVSKKIKIFEFDALLNDRVDIHYPIVEMPSKSKLSCVCWNNYIKNYLASTDYDGTVQLWDASTGQGFTQFTEHRKRAWSVSFSEVDPTKLASGSDDCCVKVWNINQKNSIDTIRNVANVCCVQFSPYSSRMLAFGSADYKTYCYDLRHTRIPWCTISGHGKAVSYVRFLDAETLISASTDNTLKIWDLNRTNPNGLSTNACSLTLSGHTNEKNFVGLSVHDGYITCGSENNEVYSYYKSFPMPITSHKFGSIDPITGQETNDDNQQFVSSVCWRGRSNMVVAANSSGSIKVLELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYG6</accession>
    <name>A0A8I6WYG6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYG6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0339230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF99">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="62"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="383" mass="42477" checksum="3F41559741D1D15F" modified="2022-05-25" version="1">MEGVNGGRRDVGEGGGTVGSSSIGRRGGLAGYPAPLATLLVLDDADVFRDEHTRLLVNTRSSRFLRKKLPLLAGHRYRHVATTPGGFFVLADGDDYDAARVLNPFMGHMIHFTATVPSDMVDAAAVIGSSPTLVLLCGGSDRKRYMAEPDSRSFALYQEEDSYPLIRLAAAGGMYADGEQRPMPPLPAAVADNIFDLMRVFSVDPSQMFWSDVVDDAPAPEIGHANNNRCYLVQSLGEVFIVFKLQHRMEVFRMDDSDRGVLKPVKNIGRRAIFVGYCRCLAVDADKFPSVEVNCIYHLVGMNPYGHFYFHDVYKYDLEDGTEEIVSEAINWSDPIIWRDADPPFTSVQLLSSYTINVRCSELVIPEMGRLDVDQLVEDFFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0S2</accession>
    <name>A0A8I6X0S2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123424447</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044964004.1">
        <property type="nucleotide sequence ID" value="XM_045108069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0S2"/>
    <dbReference type="SMR" id="A0A8I6X0S2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101260"/>
    </dbReference>
    <dbReference type="GeneID" id="123424447"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0083500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0083500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0083500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0101260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424447"/>
    <dbReference type="OrthoDB" id="67155at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF3">
        <property type="entry name" value="ELMO_CED-12 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELMO" evidence="2">
        <location>
            <begin position="115"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="295" mass="33484" checksum="8F571F3B49A86E48" modified="2022-05-25" version="1">MGIPFQLQSQQGMHQAKALLAENKIMIKKESSPSEPVGGEKPVKNASTPKPNMATAAKVNAMVGNRTWFGGLFNGSGKRRQVSADKNFEMTPLQEQRMQKLKDRLNIPFDETRPDHLKSLKALWKISFPETELTSLVSEQWKDMGWQGPNPATDFRGCGVVSLENLLFFGRRYPASFQKLLLKTQGLRATWEYPFATAGVNVSHMLIQLLELNSARPKTLPGINFVKMLSEHEEAFDILYCIAFEMMDAQWLAMRASYMQFNDVLEATKAQLERELSLEDLQRIQDLPAYNLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X246</accession>
    <name>A0A8I6X246_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X246"/>
    <dbReference type="SMR" id="A0A8I6X246"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0298670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="18969" checksum="ED999E4CBF4329E7" modified="2022-05-25" version="1">MSWSSGHSAVAAPGFRRATGRRGEESRSPVPYRESPMAYEPAKFCWCNPRRKAPRWISWSIPNPGRRYYACIDAMFLRDLIGDLRDEVRRLRCGGNGSQFIPQFEDATPILAVPEAQIDREVLAMSLQDQLKEKNAEIATLKRKYHNVVFVFALFVLGLVAGKILLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3D4</accession>
    <name>A0A8I6X3D4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123438056</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971684.1">
        <property type="nucleotide sequence ID" value="XM_045115749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3D4"/>
    <dbReference type="SMR" id="A0A8I6X3D4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0055580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0055580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0055580"/>
    </dbReference>
    <dbReference type="GeneID" id="123438056"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0043790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0043790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0043790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0055580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0055580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0055580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123438056"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140694">
        <property type="term" value="P:membraneless organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033044">
        <property type="term" value="P:regulation of chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000026">
        <property type="entry name" value="Centromere-associated protein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="3"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="356"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="543"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0055580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="639" mass="72018" checksum="3AE23131D56A4066" modified="2022-05-25" version="1">MEKISVAVRFRPPKLSVDANADSSSFSGGGAVGNREWRIDDSRISLLHRAVPVTGTSFVFDHVFNEAVTNARIYDLLVRSLIRAAVDGFNGTAFAYGQTSSGKTFTMNGSDADPGIIPLAVRDIFDTAAKAADREFLIRVSYMEIYNEEINDLLTLGSEKLPIHESLERGVYVSGLREEIVNNAEQVLQLLELGEANRHFGETNMNVRSSRSHTIFRMVIESSAKHQMNSGDVIRLSILNLVDLAGSERIAKTGADGVRLKEGKHINKSLMILGNVINKLSENGKQRGHIPYRDSKLTRILQSALGGNAKTSIICTAAPEEIHIEETRGTLQFASRAKCVSNCAQVNEILTDAALLKRQKLEIEELRKKMQGSHSEVLEQLILKQRNDMHKSELERDRLAMELEEERRLRLTLEHRLAEQKMSQNHNNADISVEQFADSSQLDTLKTPDSKRTPDGFVASRSRYLKDAEFSPIPENLGFVADENLWMQLNKGCITDLDMLEMRSDYKCPPSLEDTTTIVPLEEPIDVRCQRLETACSSDRQQLEDSKGRCTTLEKKLELLKEENSSLRQELCKSKHDTDHEKEVLLKELETERYKMEELKQDIRLFSQAFSQRQGQLTSLYTKSKAVVENCKASQVEFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X5A4</accession>
    <name>A0A8I6X5A4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123430469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970276.1">
        <property type="nucleotide sequence ID" value="XM_045114341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X5A4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178210"/>
    </dbReference>
    <dbReference type="GeneID" id="123430469"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0147420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0147420.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0147420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0178210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430469"/>
    <dbReference type="OrthoDB" id="1885878at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36743">
        <property type="entry name" value="OS04G0495300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36743:SF1">
        <property type="entry name" value="OS04G0495300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="182" mass="18643" checksum="E09D2ACEEBCDF31E" modified="2022-05-25" version="1">MTEPQRSPFSRHVAQIQSTKMGLSFSKRVDAALRRAPAFAVACDAAFNRCLADAQHAFSGVRPYQLADASAHLHSALRGSLPLVRRWVPSPPPRVRVDSALRAAGLEGAGELSRAQFGEFAAELFREAVLAGAAEAALVRAPAGAAGVLGVAIVSRAGAGAAGKLVAAYAAGVAAAVYLSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6Z3</accession>
    <name>A0A8I6X6Z3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123443534</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975879.1">
        <property type="nucleotide sequence ID" value="XM_045119944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6Z3"/>
    <dbReference type="SMR" id="A0A8I6X6Z3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0257630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0257630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0257630"/>
    </dbReference>
    <dbReference type="GeneID" id="123443534"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0213970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0213970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0213970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0257630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0257630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0257630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443534"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="64"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0257630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="245" mass="26817" checksum="7F04E7F1A12592E3" modified="2022-05-25" version="1">MPLAIGERLEMVYARPARPISSGRTGGGKSGHAAVVVAFLLDPPSCLLAIHSTGRRNPPHAMGDHYGTLGVRRDATKAEVKAAFRLRALRDHPDRHAQSGDAGARAEAARRFRQASDAYHVLSDDRRRAEYDLRLRSSSYVRTSSTTWASSSGSHGYGYGHRESSWRRPPPGGGRASAGYHWGLLLKAMTRRRFLLNLGFSSVLLSGAAFLDGSILELWNMNNSGKSFEEAMESIEKVKGEKGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8T9</accession>
    <name>A0A8I6X8T9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanine nucleotide-binding protein subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">WD40 repeat-containing protein 80</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447025</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">G proteins are composed of 3 units, alpha, beta and gamma. Interacts with the gamma subunits RGG1 and RGG2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979515.1">
        <property type="nucleotide sequence ID" value="XM_045123580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8T9"/>
    <dbReference type="SMR" id="A0A8I6X8T9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333760"/>
    </dbReference>
    <dbReference type="GeneID" id="123447025"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447025"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000580">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="153"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="201"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="340"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="41699" checksum="E26B84F79B5FA3B0" modified="2022-05-25" version="1">MASVAELKEKHAAATASVNSLRERLRQRRQTLLDTDVEKYSKAQGRTAVSFNPTDLVCCRTLQGHSGKVYSLDWTPEKNWIVSASQDGRLIVWNALTSQKTHAIKLHCPWVMTCAFAPNGQSVACGGLDSACSIFNLSSQVDRDGNMPVSRVLTGHKGYVSSCQYVPDQETRLITGSGDQTCVLWDVTTGQRISIFGGEFPSGHTADVLSLSINSLNTNMFVSGSCDTTVRLWDLRIASRAVRTYHGHEGDINSVKFFPDGQRFGTGSDDGTCRLFDMRTGHQLQVYNREPDRNDNELPIVTSVAFSISGRLLFAGYSNGDCYVWDTLLAEVVLNLGTLQNSHEGRISCLGLSSDGSALCTGSWDKNLKIWAFSGHRKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XA58</accession>
    <name>A0A8I6XA58_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Beta-glucosidase</fullName>
            <ecNumber evidence="3 8">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123445060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 8"/>
    <dbReference type="RefSeq" id="XP_044977920.1">
        <property type="nucleotide sequence ID" value="XM_045121985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XA58"/>
    <dbReference type="SMR" id="A0A8I6XA58"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305780"/>
    </dbReference>
    <dbReference type="GeneID" id="123445060"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0255350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0255350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0255350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0305780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445060"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009866">
        <property type="term" value="P:induced systemic resistance, ethylene mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990641">
        <property type="term" value="P:response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000022">
        <property type="entry name" value="Beta-glucosidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017736">
        <property type="entry name" value="Glyco_hydro_1_beta-glucosidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03356">
        <property type="entry name" value="BGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF310">
        <property type="entry name" value="BETA-GLUCOSIDASE 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="436"/>
            <end position="437"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="483" mass="54914" checksum="B8CDF1AF771F5AC1" modified="2022-05-25" version="1">MGSTAGEVTRADFPEGFVFGVATSAYQIEGGRNEGGKGDSIWDVFTDNKERVLDGSSGEVAVDHYHRYKEDIELMAKLGFGAYRFSISWSRIFPDGLGTEINEQGVAFYNNLIDFMIEKGIQPYATLYHWDLPHNLQRTMGGWLSDKIVEYFALYAEACFANFGDRVKHWITINEPIQTCINAYGIGIFAPGLCKGVSAEPFLAGHHQILAHAAAVDVYRRKFKAKQGGQVGFVIDCEWAEPMSDKMEDQAAAARRIDFQLGWFLDPIYFGDYPESMRQRVGEHLPKFSEKDRELMRNKIDFIGLNHYTSRIIGHQPNPQPEEIHFYQVQQIDRTDKWSSGEAVGERAASEWLLIVPWGIRKTINYIVKKYENPIIYITENGMDDEDDPSAPVDQFLNDTRRVNFFKGYVGGVAQAIKDGADVRGYFAWSFLDNFEWAMGFTKRFGIVYVDYKNGLSRHPKASAMWFSRFLNGEAPDSKPDTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAW8</accession>
    <name>A0A8I6XAW8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123426857</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044966691.1">
        <property type="nucleotide sequence ID" value="XM_045110756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAW8"/>
    <dbReference type="SMR" id="A0A8I6XAW8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171990"/>
    </dbReference>
    <dbReference type="GeneID" id="123426857"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0142110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0142110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0142110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0171990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426857"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000022">
        <property type="entry name" value="probable protein phosphatase 2C 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="56"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="444" mass="47551" checksum="5BB03148E84EDB4E" modified="2022-05-25" version="1">MAVEAAEARTPRRRHGSMALGDLLLREASAERADAAGGERPGVAAGQAARARKGEDYALLKQSCERHPGASFSAFAMFDGHNGAAAAVYAKERLLANVLGCVPAHLTRDEWLAALPRALVAGFVKTDKDFQTRAHSSGTTVTLVIIDGSVVTAASVGDSRCVLEAEGSIYYMSADHRFDASGEEVGRVTECGGEVGRLNIVGGAEIGPLRCWPGGLCLSRSIGDQDVGEFIIPVPFVKQIKLSSAGGRLIISSDGVWDALTAEMAFSCARGLAPEAAADQIVKEAVDSKGLRDDTTCIVIDIIPPEKPKSTIHSRKKARNGFSLLKNIFIRKKTSDTLSHADTERTSEPDLVEEVFEDGCPSLLRWLDSEYPIRNMFKLFVCAICQVELESGQGISIHEGSSKPGKVCPWDGPFLCHSCQEKKEAMEGKRPSRDSSSRNSGSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XB14</accession>
    <name>A0A8I6XB14_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XB14"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122830.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0122830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0101210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0101210.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0101210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0122830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0122830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0122830"/>
    </dbReference>
    <dbReference type="OMA" id="WAISKWR"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="141" mass="16167" checksum="390DC329BD8BD261" modified="2022-05-25" version="1">MNYNVPTFRFPPAVPERRYPAGIIVETMLCVWAISKWRGPREFSQAFLHAGFGHLPAGSPVMFHLEEICPNASSPPTVLLANFTNHFDAFYLVGKMFWCGCEFIAFTTYNIFTNIDNIFPTLNAMHSLPYYIDENGKEQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDM9</accession>
    <name>A0A8I6XDM9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Poa p IX/Phl p VI allergen family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDM9"/>
    <dbReference type="SMR" id="A0A8I6XDM9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330410.1">
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        <property type="entry name" value="Group V grass pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035506">
        <property type="entry name" value="Pollen_allergen/Os"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035216921" evidence="2">
        <location>
            <begin position="30"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="128" mass="13573" checksum="2AFC189AF08C2B75" modified="2022-05-25" version="1">MAPLQNIAVALTLLIVMVEIASLPMLASATIVKSEEAALDELTPIIKTALDGVLATAPPSERIKVAGAVAKQELLAMDTMKKAKGDKAKFDTHLLAYKIAAKIVTAAAPTEKFKKMEDSFTEASRPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XE77</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6XE77"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0127010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="26848" checksum="8AADD9AD5E5A1F7A" modified="2022-05-25" version="1">MATFIPVKFTEFWQPDATMPFGKELVQITKDLRIALPTITGKPTSSSPEDSCYRIEVHVPGRTFQPRTEPMDFKFIAPNWILGRDMAIHCTLGRIKEEYKGCPISLDLFTVSRRNEDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLCELELEEAAREAHYEHELEAKDFLERIEKLKARVAYLEEELDMGENLHLEGDVAPLISNDEDYEESSTEGPATMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEE7</accession>
    <name>A0A8I6XEE7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEE7"/>
    <dbReference type="SMR" id="A0A8I6XEE7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0272260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0272260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0272260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0225600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0225600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0225600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0272260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0272260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0272260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17964">
        <property type="entry name" value="DEADc_MSS116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="196"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="409"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0272260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="633" mass="69178" checksum="FAD58B304B6CC2B7" modified="2022-05-25" version="1">MKAASDEAGEISYLLARQAANSQGQNPLPLLPKPYRPSHSPEMAGGDILLRTHGGIPVLVRASPCSLRLRLRFTAGGRRAPSSLAAAKVDIGDVVSQLRSGSARGASVTRRQRRDPEGGLAFPRVVTSRIRGADEEEGEGKALQLDAEKVGGEAGGVDGSYLSETRFDQCAISELSLKGVKDAGYGRMTQVQEATLPVILQGKDVLAKAKTGTGKTVAFLLPAIEVLSKLPSSQRSHLRSSVNLLVMCPTRELANQVAVEAKKLLKYHRLLGVQVVIGGTRITLEQRNMQANPCQILVATPGRLKDHLENTPGFSTRLKGVKVLVLDEADRLLDMGFRRDIEKIMAATPKDRQTLLFSATVPEEVRQISHVAMRKDYRFINTVKEGDEETHSQVSQMYMIAPLDLHFSILYDVLKKHVTEDADYKVIIFCTTAMVTKLVAEVLSQLNLNIREIHSRKSQSARTKVSDEFRRSKGVILVSSDVSARGVDYPDVTLVIQVGIPSGREQYIHRIGRTGRKGKEGQGLLLLAPWESHFLTSVKDLSVSEAVTPSVDSSTQTEVKGALRRVEMKSKESAYQAWLGYYNSNKTIGGNKSRLVSLGEEFSRSMGLAAPPAIPKLILRKMGLSKVPGFRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG92</accession>
    <name>A0A8I6XG92_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG92"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0191630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF43">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="3">
        <location>
            <begin position="243"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="538"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="331"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="575" mass="62245" checksum="88A0632885587BA9" modified="2022-05-25" version="1">MTTPTGGVASAPSHAAAAPLERKKGNTSTKKKTTDGSGSSKLKKKVAVRRPGAASTEAPASSLVEPAADAHHVFDEMPPSLNDDAYMSTVGVGSNNSHWSQTNDIHLDDHEFEVDEEGEGIIEAPKGRAGNYTTNDDKLLCNTWLQVSRDPSVGGDQSRDAYWGRMKEHFDAQNMSGIDLFERSLRSRWSTINSDCQKWAAAQKAVDKLNPSGTNEDDRYNISQNLFKEETRTTKKGKIKKGKIFTLPHCYEVLKDDEKWKKREDLDDLHLSNKRKRTIELNDDEEEEDASTDDGKRSPTPNSVSYSKPKRPDGCKKDKTEKKKRKGDDELTNAMEAIVKARKEANEVRKMARNQDAAAEERRLAAEERRVAAEERKVALEERKVGMEERAKLLEWEKHLFFLDTSLFNDAQKEYVNLAREEVLIQKRAMIRTMGGGGLGGMGGGGLGGMGGGGLGAMGGMGLGAMGAMGGGVLGGMGGGGFGAMGGFGAPSNAMGGMGGMSFASLMGGMGAPPAMGGMSFDVPHTHTHENAVEDLAKTVGATRDVVRDEVREEDSSSKAEESSSKEEEDDEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK66</accession>
    <name>A0A8I6XK66_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123439421</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972073.1">
        <property type="nucleotide sequence ID" value="XM_045116138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233680"/>
    </dbReference>
    <dbReference type="GeneID" id="123439421"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193940.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439421"/>
    <dbReference type="OMA" id="NNDMAID"/>
    <dbReference type="OrthoDB" id="1922547at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304:SF73">
        <property type="entry name" value="OS01G0511000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="282" mass="29701" checksum="223CAB8CB51AC101" modified="2022-05-25" version="1">MRMSCNGCRVLRKGCGEGCTIRPCLEWIRSPDAQANATVFLAKFYGRAGLLNLLAAVPDAGLRPPLFRSLLYEACGRMVNPVYGSVGLLWSAQWEACQAAVEAVLKGRPIVRVSSDAPLAPCDIRHVAKPDRPAAAGTLPGVSRAGRTRFKRASSSTAKTKSSFSDANKHDDGLDQAPSHEESAGSHVEDGGMAVEQAREEESSEGTEVDAGSHVSQAEHSPAPPVAKDEEAHGDEIGLELTLGIQTVAPRLVVRSPPACFGASSSNAQSSHIGLLLELPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKR6</accession>
    <name>A0A8I6XKR6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123426747</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044966563.1">
        <property type="nucleotide sequence ID" value="XM_045110628.1"/>
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    <dbReference type="SMR" id="A0A8I6XKR6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0168830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0168830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0168830"/>
    </dbReference>
    <dbReference type="GeneID" id="123426747"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0168830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0168830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0168830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426747"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001454">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000804">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="E_motif"/>
    </dbReference>
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        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF485">
        <property type="entry name" value="REPEAT-LIKE SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0168830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="67600" checksum="1B26EE235C9C2397" modified="2022-05-25" version="1">MRLLGVKLPAICATTKPPMPSSPAPRRRPPAAATSTALLPRLRLAVGHTSPRRLLLQSLALVLTSNLSSTHAAVSSRLLNSLLPHLPASHPHLLRFLPFDHLTLLLLSSGSPNRHTIYPLPAASSLHALAVRSGHLPADLRLANSLLARYLAHGSHASARRLFADVPRPDAVTWNTLLRACLRSGLLPSARHLFDQMPVRDLVSYNSMLSGHAAAGDMDGARQLFDGMPERDVVSWNSMLAGHTRCGDMEEARRMFDAMPERDVVSWNSMLDGYAQAGDVLAARAVFDGMPRRSTVSWNVVLALYARVKNWRECLRLFDAMMAVGAAVPNEKTFVSVLTACGSLGDLERGKRVHDLVRQRWETLVPDVLLLTALLTMYAKCGVMEAARVIFDSMGERSVPSWNSMIIGYGLHGQSEKALELFLEMERTGPRPNETTFVCVLSSCAHGGLVLEGWWCFDRMVRLYGVEPKAEHFGCMMDLLGRAGLLTDSDTLVQNLQEKASPALWGAMVSASRAQNGSRLGEFVGKKLIEMRPKEVGPYVLLSNIYAAEGRWNDVEKVREMMKGNGAEKDVGLSLVGSSEPEHRIGTEDGVVLSMLGEIGAHLKLPSVEPKRMEGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKS0</accession>
    <name>A0A8I6XKS0_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKS0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0457300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0457300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0457300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0378530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0378530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0378530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0457300.1">
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
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    </dbReference>
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        <property type="entry name" value="MYB_DNA-BIND_3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46934:SF12">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
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            <end position="294"/>
        </location>
    </feature>
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            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0457300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="294" mass="33069" checksum="CE912F77CEACD5F9" modified="2022-05-25" version="1">MMTARGSPRLNLMKEVAERHRASPPTGSVEKRRRGSPKEDRAQWNASLEKDLVDFLREHATPEHKGQNGWSSEAWNKIVKKFHQKNPYARYEKKKIQEKETELKRDYMMIKEIRKQSGVSWDDQQCKILADPPLWKNIIISHPKAGKFKTKGFPLFDALGELHDGQTAEGTYNFTSIESSQYSTQAQFENLGGAGEYLRENHGETSYHSDNIGGEPSANGENLGGERVQIDEDVEEVFVQENIVVEPHQTQPSLATAPSRNGQEKEPKRRRGANSDVAANDGEVLGNKGEAGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLS2</accession>
    <name>A0A8I6XLS2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLS2"/>
    <dbReference type="SMR" id="A0A8I6XLS2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0368920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0368920.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0368920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0445430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035260342" evidence="1">
        <location>
            <begin position="30"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="108" mass="11713" checksum="F7C44A9322D3E5C4" modified="2022-05-25" version="1">MAVNTRNTIGVVLLLVVMVAAVSTPVATAEEFCWDKCIKRCKGELEVCSPKCIHFCNYQSGAIGYVKWAADKLKEAAAAPPEKVVLLKNEAQTYLERANALNEKAGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM07</accession>
    <name>A0A8I6XM07_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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                <person name="Muehlbauer G.J."/>
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        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF35">
        <property type="entry name" value="2FE-2S FERREDOXIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="16962" checksum="41B8287022A17B86" modified="2022-05-25" version="1">MAIAARALRRLPLHLAPALARPFCAVSPAAAAPAPSAKVADRIVRVLAIDLDGGRREVVGLAGQTLLRALVNAGLIEAASHRLDDIDACSAECEVHIAQEWLEKMPAASYEERYVLTRASRNRELNKHARLGCQVVLGKEHQGMVVALPEPKPWDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS02</accession>
    <name>A0A8I6XS02_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5'-3' DNA helicase ZGRF1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS02"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0475670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0475670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0475670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0394020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0394020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0394020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0475670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0475670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0475670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052800">
        <property type="entry name" value="DNA_Repair_Helicase_ZGRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018838">
        <property type="entry name" value="ZGRF1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535:SF1">
        <property type="entry name" value="PROTEIN ZGRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535">
        <property type="entry name" value="ZINC FINGER GRF-TYPE CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10382">
        <property type="entry name" value="ZGRF1-like_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="2">
        <location>
            <begin position="145"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="2">
        <location>
            <begin position="313"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0475670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="761" mass="82514" checksum="9DDF1DEA97A68F87" modified="2022-05-25" version="1">MDPEPRQWAATYTTQLRQKRKAYHDGVLLLHPDSRRLVLLDDAGVTIDARSLREAEYVSEGASLEFPCHLVDVGEAQRVPTTARPGRPSSESAAPRTAYRGGARARHGAANTCAPRAFVNPPKRGAGKTEAAGSGCARSAGSTFQEWSALYTTQVSQKAKKYHDGFVKLVQSGLYSKQIVLLDEDGQTLGTRHLKSGECVESGKKCSFPGYLIEIGEATNLNKGGEPKSSEEPMVQTRPKRVENANSKMGIGTTSSLKFISPQKSIQFVGFDESKSEATSSSNKPELGKSEVAAAGSTGSLMGSADSSSKEIEWSVLYTAQLNQKAKKFHDGVIRLVQAGPNAKQIVLLDEEGGVLGTRYFNSGESIECGKRCQFPNYIIEVAELRNKMKDVEHKHATGEASSHTEKKDDKSKSPKFVSPLKFHDHRKNKTRSTTDSNKPQAGKSTCSNMGDPLNFHVFTDLQRGKPDCTVGYGRAYPGSTSSDVDNQHKFNDNQRGTSEFSTSYSRLEVEKSTFNGPGKSTLGGMDDPHIFDDIQRCKSGFSTSYNRPEVDKSILNRMDDPLKFCGLQDGKSVCPTSFVGREVGKSTIGNADDSLRTASQILSIMKPPAGLSHFATQLRTSVQSCLKLDTAHAKNSVIAHNRNGLPGNAPTYNHQAIMKPPAFDASDLELIDLPESEMCNANEQKLESSGNHNTGNCNRTASAPILNVTSIPDLREDKSGTADQLGGNNTAEDPKYDSGACRDSTIQDLIDDCPSFDLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU12</accession>
    <name>A0A8I6XU12_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SUZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XU12"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0584610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0584610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0584610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0584610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0584610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0584610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF8">
        <property type="entry name" value="PROTEIN ENCORE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="3">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ" evidence="4">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0584610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33889" checksum="FC134FD517B7640D" modified="2022-05-25" version="1">MKMLQGLVPGCNKVIGKALEKPRERLSVLRMEQDILKFLRDPGQTQFEFQGLPTSYLHLAAHRLAQHYFLISIALPDNSLPDGTSSQIILRKTSAECRLPVVRLADIPVNLPQEETSAVATKVAIKQRPRKNHHGGAGAGANSSRGNLQKSVEERKEEYNRVRARIFNNISGSSSPVDGRPADEVVFPNTLHRSTSLELNPNTRYGELAEATLERSLTSTASSSRSNNRSRIDKEPPVNRGRQGNRVAIFRDRDSDRKDPDYDRSYDRFVPHNMASVCCYLIWGFLCRNKEYLICTVPTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWW4</accession>
    <name>A0A8I6XWW4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWW4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0237610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0237610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0237610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0286310.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286310"/>
    </dbReference>
    <dbReference type="OMA" id="SKFCVSP"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="142" mass="15479" checksum="6A8078505AFEC4F4" modified="2022-05-25" version="1">MLREEQEEEEEFHEADILWPDAAQEPDLAETRYSHDDGDGDGDDDGCSGEYRRPTKAPNWQKASSPIDIPGKTGRATGAKPQAPQAGFSKFSASLAGSVMIGSQVFVPPHVIVDRRAKRDAAMLMLLDAPKVGRVRATVMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XX25</accession>
    <name>A0A8I6XX25_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044972722.1">
        <property type="nucleotide sequence ID" value="XM_045116787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XX25"/>
    <dbReference type="SMR" id="A0A8I6XX25"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287070.1.CDS1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123440216"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0238350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0238350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0238350"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287070"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000020">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000082">
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    </dbReference>
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        <property type="entry name" value="UDP_glucos_trans"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287070.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="475" mass="51331" checksum="1B5BF15AF1482ADB" modified="2022-05-25" version="1">MKKTVVLYPGLGVGHLVPMVEVAKLFLKHGLAVTMALVEPQVKSTDFSAAVARARASNPSVAFHVLPTPTPPPPADANTDGAPRHHVVKVIQSLAAMNAPLRDFLRSLPSVHALVLDMFCVDALDVAAELKLPVYYSFASGAGDLAIFLNLPSKFASNTAKVKELGDSIVTFPGVPPFKASELPSEVIGDGEAFMYLLRMFERMTEANGILINSLESLEKPAVTALNDGLCVTGRATPPVYCIGPLVSGGGDKEHDCLRWLDAQPDQSVVFLSFGSLGTFSSKQLEEIALGLDKSGERFLWVVRSPRSPDQKHGDPLPEPDLDALMPEGFLERTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVDGMKLGVEMRGYNEEVVKAEEVEEKVRWVMASEGGKALRERVTAAKDAAADALKEGGSSYLAFDQFLNDMGTSMVATVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2H2</accession>
    <name>A0A8I6Y2H2_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123449717</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044982966.1">
        <property type="nucleotide sequence ID" value="XM_045127031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2H2"/>
    <dbReference type="SMR" id="A0A8I6Y2H2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408100"/>
    </dbReference>
    <dbReference type="GeneID" id="123449717"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0408100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449717"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-conjugating enzyme 5, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="184" mass="20876" checksum="0BFFD595B068BBC7" modified="2022-05-25" version="1">MSSPSKRREMDLMKLMMSDYKVETVNDGMQEFLVEFRGPEESLYQGGVWKVRVELPDAYPYRSPSIGFVNKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAALLMRDRPAYEQKVKEFCEKYAKPEDAGITPEDNSSDEELSEDDDDSGDDEPILGHADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3J5</accession>
    <name>A0A8I6Y3J5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3J5"/>
    <dbReference type="SMR" id="A0A8I6Y3J5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF112">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE CONSERVED REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035228501" description="Strictosidine synthase conserved region domain-containing protein" evidence="5">
        <location>
            <begin position="32"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="155"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="323" mass="35123" checksum="71D1DB1BBA5B72B7" modified="2022-05-25" version="1">MASGTRGVAAATISLTVLLLIFCFSPSTAAAAAIPSIDATRTRHLPLPRGLLRGPESVAFDAKGHGPYSGVSDGRVLKWNGDALGWSTYTYNPNYSSEACTASLLRPETATESHCGRPLGLRFHLKSGYLYIADAYKGLMRVAPGGGEATSHRVDSSMNFNRSQHEMVTRTGDSSGRLMRYDPRTGKAVVLQAGITYPNGLAISADRTHLIISSTGPCKLLRYWIKGSKAGTMELFADLPGYPDNVRPDKKGGYWVALHREKNELPFGVDSHLLALRIDAEGKIIEEMRGPKSVRPTEVVERKGGRLFMGSVELRYLSVVIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7P3</accession>
    <name>A0A8I6Y7P3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123410280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959122.1">
        <property type="nucleotide sequence ID" value="XM_045103187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7P3"/>
    <dbReference type="SMR" id="A0A8I6Y7P3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0639640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0639640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0639640"/>
    </dbReference>
    <dbReference type="GeneID" id="123410280"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0531430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0531430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0531430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0639640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0639640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0639640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410280"/>
    <dbReference type="OMA" id="FCTDLHK"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF607">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035291242" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="40"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0639640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="397" mass="44201" checksum="1A535DD42D84DA20" modified="2022-05-25" version="1">MAAMWSPIIGLLLLLLQLAPSSSSYIQLPLQGNVYPVGYFFVTMYIGGKPYSLDIDTGSNLTWLECLHPNHQCKICKGPHPPYRPNRSHKMVNCGGQFCTDLHKDLPPYQDPPCHARQQHECHYHVRYVEGTSSDGFIVNDTVFVDVFAPSKRVQIFFGFGCGCNQQPQKYSPVDGILGLGMGKVGFVAQLKAHNVITKDIIGHCLSIDGGGYLFIGDYNFPAGVSWAPMRKYSHYYSPGPAQLLFGRKKIGSKTMMVFDSGTTYTYIPDGVYSQLLAEVKQSVSRSLERVTDQALPVCWKGPFASIQLVKGKFKPLALEFTNQVMHIPPENYLTLTQKKNVCLGILQTPKAGGQMMIIGDVTMQDLLVIYDNVDKRVGWVSKQCKHNLEIPSIRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y823</accession>
    <name>A0A8I6Y823_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y823"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643510.1">
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
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        <property type="project" value="InterPro"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643510.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="91" mass="9901" checksum="DAC4EDB3990E0943" modified="2022-05-25" version="1">METDSLEVTNIWATRHDSRSVAAPILLEIEELATSFNSFVICHIVRSANGPAHICAKHACTINMTESWIDSTPSLLINSLLANCSASAFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y862</accession>
    <name>A0A8I6Y862_HORVV</name>
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        <recommendedName>
            <fullName evidence="8">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the peptidase A1 family.</text>
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        <property type="component" value="Chromosome 5H"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Aspartic_peptidase_A1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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            <end position="493"/>
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            <end position="441"/>
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        <location>
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            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YB69</accession>
    <name>A0A8I6YB69_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
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    </protein>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YB69"/>
    <dbReference type="SMR" id="A0A8I6YB69"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0500330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0500330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0500330"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0415290.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46934">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <end position="137"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="228"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0500330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="228" mass="25978" checksum="21EFA721B32618F7" modified="2022-05-25" version="1">MMTARGSPRLNLMKEVAERHRVSPPTGSVEKRTRGSSKEDRAQWNASLEKDLVDLLREHATPEHKGQNGWSSEAWNKIVKKFHQKNPYARYEKKKIQEKEKELKRDYRMIKEIRKQTGVSWDDQQCKIPADPPLWKNIIISHPKAGKFKTKGFPLFDALGELHDGQTTEGTYNFTSIESSQYSTQAQLENLGGAGEYLRENHGETSYHGDNIGGEPSANGENLGEERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBB9</accession>
    <name>A0A8I6YBB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123400203</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950562.1">
        <property type="nucleotide sequence ID" value="XM_045094627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBB9"/>
    <dbReference type="SMR" id="A0A8I6YBB9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0501170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0501170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0501170"/>
    </dbReference>
    <dbReference type="GeneID" id="123400203"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0415990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0415990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0415990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0501170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0501170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0501170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123400203"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000012">
        <property type="entry name" value="Aspartyl protease family protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000014">
        <property type="entry name" value="Aspartyl protease family protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF232">
        <property type="entry name" value="OS09G0542100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035323115" description="Peptidase A1 domain-containing protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="97"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="451"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0501170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="518" mass="54674" checksum="8CE6DD3952FE1181" modified="2022-05-25" version="1">MGAAARALLLLALVAAGAEALSLDVHHRYSATVRGWAGLRRGPSPGTAEYYAALAGHDDLRRRSLSLAAAPAPGAGGPFAFVDGNDTYRLNQFGFLHYAVVALGTPNVTFLVALDTGSDLFWVPCDCLKCAPLSSPDYGNLKFDVYSPRKSSTSRKVPCSSNMCDLQTECSAASNSCPYKIEYLSDNTSSKGVLVEDVMYLATESGHSKITQAPITFGCGQVQTGSFLGSAAPNGLLGLGMDSKSVPSLLASQGVAANSFSMCFGEDGHGRINFGDTGSADQLETPLNIYKHNPYYNISIVGAMAGGKTFSTKFSAVVDSGTSFTALSDPMYTEITSAFDKQVKEKRNPADSSLPFEYCYTISSKGAVSPPNISLTAKGGSVFPVKDPIITITDISSSPVGYCLAIMKSEGVNLIGENFMSGLKVVFDRERLVLGWKSFNCYSVDHSTKLPVSPNSSAIPPKPVSGPGSSNPEAAKRPSPNITQIDAAKPSSGSSTLFHFSSRTFFFTAITPLFLAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBN0</accession>
    <name>A0A8I6YBN0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBN0"/>
    <dbReference type="SMR" id="A0A8I6YBN0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0596840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF72">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="286"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="454" mass="50784" checksum="41B8579EF721575B" modified="2022-05-25" version="1">MDHVMKQKSQIFFVDGPGGTGKTYLYKALLAKVRSMGQIAIATATSGIVASIMPGGRTAHSRFKIPIKLTNNTMCSFTKQSGTAELLKQASLIIWDEVAMTKRQAVETLDRSLQDIMGCPLPFGGKVVVFGGDFRQILPVVTRGTRAQITDATLLRSYPWEKIRKIRLTCNMRAPADPWFSEYLLRIGNGTEETIGDDYVRLPNDIVIAYTEDETAMNKFIEDVFPSLHANAKSREYMSTRAILSTKNDHVDALNDKMISRFPGKEKLYHSFDSIEDDMQNNYTIDFLNSITPNGLPPHVLRSKVNCPVILLRNLDPYNGLCNGTRLMIRAFQDNAIDAEIVGGQDAGKRVFIPRIPMSPSEDISLPFKLKRKQFPIRLSFAMTINKAQGQTIPNVGIYLPEPVFSHGELYVALSRGVSRQTTRILAKPNKEVDKSGKSTKNIVYRDVLRDEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBZ6</accession>
    <name>A0A8I6YBZ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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        </source>
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</entry>
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            <taxon>Hordeinae</taxon>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0427830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0427830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0427830"/>
    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0427830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="13223" checksum="04FF5CFE048FC781" modified="2022-05-25" version="1">MLVPQAIKMKEITPKKKKTNKPSVVVNAGKTDEEAIARFAREHPEYVQAELEHYWKREAEQKKKEDEAGTSTVIPIESSSEEDWEDFSEEEEGCDDPEKEEFWEQFRSSDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCP0</accession>
    <name>A0A8I6YCP0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513650.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0513650.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14562" checksum="58F2B9F7BECDCDFC" modified="2022-05-25" version="1">MTVAGDPKCSFCDELESAQHLFFGCSVAKIVWRTVGAVFDTSYIPKTIWQVFSWLYAFLSGLCEIYTVGLAAVCWSIWLARNRATFEKKWIKTPFEIAFTTCAFIEYGAGMQKRRWQRMSRRELSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YEB7</accession>
    <name>A0A8I6YEB7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948789.1">
        <property type="nucleotide sequence ID" value="XM_045092854.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6YEB7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485000"/>
    </dbReference>
    <dbReference type="GeneID" id="123398375"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0401580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0401580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0401580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0485000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398375"/>
    <dbReference type="OrthoDB" id="646838at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389">
        <property type="entry name" value="OS09G0436400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389:SF5">
        <property type="entry name" value="OS09G0436700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="405"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="511" mass="59142" checksum="A64F6DA1BE5E71DC" modified="2022-05-25" version="1">MPRAVRIRGDELEKTEREAKRRNLTEKEKEEEEESTALHPTSPRSPGEVAIRTSHPKILFAVFEPDDRTQLEAKQDRVVESVAEEESSSEPLSPPIYEPYLPEELADDPDILHDFKDAKARYYKERNRRIRLFTMDHHYYKAPSCLFNPHLLLPILEPARDAALRTAKSVILLSSFVGDDPLNKCSGLWFQRDDQSKTALVLTSAQLIREKNPKKPSEWRQQWTGKYLRDAEVFVHFLDNTAAPGRLLYLQEHYEFALYEFEVDKPVQLLTFTERVCCGQDVLRLGRDESLDLRISHGMVAHKIPSRHERCHYMYFSRDPSSRLLLQDDGGAIIDLEGKVVGLVNNHIVETFVPSFILHRCLDFWRRFNCMPRLHLGMAFTSIKHLDPICIERMTHDHNIESGLIVEQVSKGSNAEKIGIREGDVIESFNGKYVSTTIELEQMLIDICWDHFHQGKCLYEEKDVSVKIFDAIKHRRRTRNLTVIVSDLGEDIVKCTYPFMGKEAMSVSYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YEW6</accession>
    <name>A0A8I6YEW6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YEW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0445040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0445040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0445040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0534920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="131" mass="14931" checksum="C06D3CE74EF55C46" modified="2022-05-25" version="1">MTITLEDIAMITGLPIEGRALTGQVRSVGWRQRVAALVGVEPEPWTHETRKNPRPSGVLFSWIHRNFRKCPKDASPAVVERFARAYLLNLLTQVVFSDGTGDTTSWMFLDPLRDWDVKWSWGSAVLAFLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJ33</accession>
    <name>A0A8I6YJ33_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJ33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580400.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0580400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25839" checksum="D224CF7F4CCF3FC5" modified="2022-05-25" version="1">MSLCGYRWYPEYTVEEQYRDINQSQYICTVRVFPDYPGAEEPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPTHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
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            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="5 6">
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        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958833.1">
        <property type="nucleotide sequence ID" value="XM_045102898.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6YM04"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731230"/>
    </dbReference>
    <dbReference type="GeneID" id="123410026"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0606750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0606750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0606750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0731230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410026"/>
    <dbReference type="OrthoDB" id="431169at2759"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
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            <end position="146"/>
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            <end position="308"/>
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        </location>
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            <end position="197"/>
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        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="321" mass="34841" checksum="417DE5FD1A49C23A" modified="2022-05-25" version="1">MAASYYNPPPPRAASSSYATLPPPPPGTSSYDTYRQHEHAYLAAPPPPAYGGYFDRAEPLAPPRDELRTLFIAGLPADVKPREVYNLFRDFPGYVSSHVRSGKSAQSYAFAVFGDQPSALAAMSATNGLVFDLEKNCSIHVDLAKSNSRSTSSKRPRSDYEDFPKSTGKKARSPRGRPDSGAGSNNHMSGMGNSSHSLNGYPAQSYTDFGSSAAFSKDPSMFAPQNNPPCPTLFVANLGQTVSERELTDVFSGCEGFIKLKMQNKFGAPVAFVDFKDDHSSTEALNRLQGAILHSSSGEGMRLEYAKSRMGLRKQRDNNRS</sequence>
</entry>
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    <reference evidence="2" key="1">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="87" mass="10383" checksum="A4F4B5B4A691DF63" modified="2022-05-25" version="1">MGRKYIIHGVTIRPNFEKLECRFINHTYECSFSARTFVESLSLEFPRYPKHLMSIHEVMRCPNKTFVGTHSNIYNTNTKITFVYQFD</sequence>
</entry>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012871">
        <property type="entry name" value="DUF1668_ORYSA"/>
    </dbReference>
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        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33085">
        <property type="entry name" value="OS12G0113100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07893">
        <property type="entry name" value="DUF1668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="370" mass="41283" checksum="66A5E7C6A4B4A3F0" modified="2022-05-25" version="1">MDDHSDDLDDGHPSSPRRFPMLPALSVARSTLSEWAKFTAVGRSIVAIGRKPQGSTGEDDGRVLIYDTKEAKLLPSPRIPQGLEYGYEAAMAVGQRLYFLESVSGLHSLHRDQVHPGGEYRYMYHGALHCLHAADPPDVDVDVEGPGYKLWSWSTDDRLLGVPFRANTITGHAVHAPPVSVAVHHDIYVSALVDTVMPKGVTFSFSTASGKWTRHGYWQLPVVGHAHYDDQLGAWLGLHYGEGSINSRRLVDGHLCAGNIASAPPEWKVGTEKLFHREEDMEAGWRNFDAKLVPMAPGEGGSEYCLMERLRPEGDVRESFGDGDKWLLRLTAFRVERGQDGLPVVTARRPVRCYKMSRYNKYFDAQAFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNW9</accession>
    <name>A0A8I6YNW9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123406357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044955801.1">
        <property type="nucleotide sequence ID" value="XM_045099866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNW9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543130.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543130.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543130"/>
    </dbReference>
    <dbReference type="GeneID" id="123406357"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0543130.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543130.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406357"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543130.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="548" mass="63007" checksum="609B2FCF8148CD9D" modified="2022-05-25" version="1">MSHRRRPHAAGPPPISEEGGGGTPPQPPGKAPRIRPWAERRVLALALAFRAANALLVRTYFNPDEHWQCLEVAHRIAFGYGHLTWEWKRGLRSYLHPLIFAALYKLLAFLHLDTPWFMAMAPRLLQSVFAAFGDLYLYKLSKLIFNEHVAQWTLFSQLVNWFMFFCITRTLSNSLETVLTLAGLYYWFIAIESSKGISVISKQQAASYQSPHSRKVALLIAALACAIRPTSAITWLYVGLLDFIYIKSKCRFLFLEVIPIGAIVLAATTFLDWWMYGSRVIVPLNFLKFNLFSSGGDYYGTHVFHWYFTQGFPSMIWTFLPLSVYGVIKSREWRISGLVAWVLGVYSILGHKEFRFVLPVLPLALMFSGYFLAAMSQFKGKNLHGKRDFSRLQLSVIFLIITNVPMALYMSLFHQRGTEDVMFYLSKEAHNGRVKGVLFLMPCHSTPYYSTLHSNLPMRFLDCTPSDNKGTLDESDRFLMNPLDFVGEVFGNLSSFSHIVLFESEERHVIQLLLHDSFQEVRRFFHSHFKIDRDLQSSVVVYSRRDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRQ7</accession>
    <name>A0A8I6YRQ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRQ7"/>
    <dbReference type="SMR" id="A0A8I6YRQ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0462040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0462040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0462040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0556170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF524">
        <property type="entry name" value="MATH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="BPM/SPOP BACK" evidence="4">
        <location>
            <begin position="77"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16133" checksum="D898FF033A17EEBC" modified="2022-05-25" version="1">MMESRESSIRVKDMDAKVLEILLHYMYNDRLPESMDEATEETTNMAQHLLVAADRYGIGRLKLMCESRLSKALDVNTVGFTLDLAEQYHCQQLKDCCLRFMTRDGGRLRAIVNTKGFAQLKRNQPHVAFDILGQVIALLPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YTT7</accession>
    <name>A0A8I6YTT7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123413071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044952288.1">
        <property type="nucleotide sequence ID" value="XM_045096353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YUY7"/>
    <dbReference type="SMR" id="A0A8I6YUY7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612680"/>
    </dbReference>
    <dbReference type="GeneID" id="123402429"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0508020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0508020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0508020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0612680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402429"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000069">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="146"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27240" checksum="1C851EE2A227F910" modified="2022-05-25" version="1">MGRQPCCEKVGLKKGPWTAEEDQKLVAFLLSHGHCCWRLVPKLAGLLRCGKSCRLRWTNYLRPDLKRGLLSDDEERLVIDLHSQLGNRWSKIAARLPGRTDNEIKNHWNTHIRKKLKKMGLDPVTHQPVAARPQDPRRQDPHCDGKASSQESAGSGAEEEAPTSGEPKQGAACSASVSPSPCSSSSASASASVATPGGADVDWPDPLDLFQVDAIIDMDWASILSGSGGGDIGVDLFDQCSDVGFDQEVWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVY2</accession>
    <name>A0A8I6YVY2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVY2"/>
    <dbReference type="SMR" id="A0A8I6YVY2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0514790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0514790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0514790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620600"/>
    </dbReference>
    <dbReference type="OMA" id="RCMQRNQ"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030380">
        <property type="entry name" value="SAM_MeTfrase_DRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF48">
        <property type="entry name" value="SAM-DEPENDENT MTASE DRM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51680">
        <property type="entry name" value="SAM_MT_DRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase DRM-type" evidence="11">
        <location>
            <begin position="214"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="540" mass="60716" checksum="F498175023594475" modified="2022-05-25" version="1">MGFPKEMVVKGIKEIGHNDANALLELLLTYKALGDEGALGNCSTSGCSRPNVEDDNDDDLDFDNWNNDQDTGGRQQNSDSSGDENFLKEMSAKDEKISSLVYMGFSEDEANMAMTRCGVDADLWVLVDSITASQVAGDCRSRNVFDHQVMDQCFDSFGERKKARLMEERKKKRMRYGGGAHGNRSSLDGSDDEPMLLPNPMVGFNLPGYKRPSMMRKLPQQAIGPPFFYYENVALTPRGVWQTISRFLYDIKPEFVDSMHLCAAARKRGYIHNLPIENRSPLLPLPPKTIFEAFPHYKKWWPSWDPRRQLRCLQTTVASPKTTERIQGLLARSGNPPPPSVQKYVMDECRKRNLVWVGKNKVAPLEPNEVEYLLGFPRDHTRGVSKTERYKSLGNSFQVDTVAYHLSVLRDMFPNGVSVLSLFTGIGGGEVALHRLGIHMRVVVSVEISEANRKIFRGWWDQTQTGTLIEIADVKSVTPDIIASYVGRFGGFDLMIGGSPCNNLAGSNRHHRDGLEGEQSSLFYHYFRILEAVKSAMARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YY70</accession>
    <name>A0A8I6YY70_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123404007</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953840.1">
        <property type="nucleotide sequence ID" value="XM_045097905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YY70"/>
    <dbReference type="SMR" id="A0A8I6YY70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620580"/>
    </dbReference>
    <dbReference type="GeneID" id="123404007"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0514770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0514770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0514770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620580"/>
    </dbReference>
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    <dbReference type="OMA" id="WIPAMIM"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
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    <dbReference type="GO" id="GO:0005576">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Peptidases_S8_3"/>
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        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PA_domain"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035312689" description="Subtilisin-like protease" evidence="8">
        <location>
            <begin position="24"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="11">
        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="141"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="386"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="12">
        <location>
            <begin position="672"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="80820" checksum="32AB70A956B60258" modified="2022-05-25" version="1">MMARPFVLLCVLLAVVVTAAASAAREKEEEPSAYIVHVAHAHAPPLPRRGLLSTRAYASFLRDHVPVDMSLPAPRVLYSYSHAATGFAARLTGRQAAHLTSQRSVLAVVPDVMQQLHTTLTPSFLGLSASSGLLPASNGASDVVIGVLDTGVYPIDRAAFAADPSLPPPPGKFRGACVSTPSFNASAYCNGKLVGAKVFYKGYEVNLGGPINETEESKSPLDTVGHGTHTASTAAGSAVPDAAFYGYARGNAVGMAPGARIASYKVCWKYGCPSSDILAAFDEAIADGVDVISASLGSSGYAEPFYMDSTAVGAFSAVRKGIIVSAAAGNSGPVESTANNIAPWFLTVGASTINRRFPADVVLGNGDTFSGASLYAGPPLGPTAIPLVDGRAVGSKTCEAGKMNASLVAGKIVLCGPAVLNAAQGEAVKLAGGVGAILTSTKQFGELAVGSPHTFPATTVTFAAAKRIKTYMNKTTSPAATIVFHGTVIGPTPSSPRMAPFSSRGPNLHAPEILKPDVTAPGVEILAAWTGAASPSGLDSDRRRVHYNVLSGTSMACPHVSGIAAMLRQARPGWSPAAIKSALMTTAYNVDSAGNVIGDMATGKASTPFARGAGHVDPDRALDPGLVYDAGTDDYVAFLCALGYTADEVAVFTRDGSSTNCSAAPGSAYVGDHNYPAFVAVLTSRNGTITQRRVVRNVGSDVVATYRATVTSPAGMRITVKPRKLRFSKTHKTQEYQVTFAIRAAGSIKEYTFGSIVWSDGKHKVTSPIAIAWSPPASEIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZ89</accession>
    <name>A0A8I6YZ89_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="RNaseH_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
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    </dbReference>
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        <property type="entry name" value="DNA_pol_A_exo1"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="47"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25070" checksum="50348BBDA61450A3" modified="2022-05-25" version="1">MPTSFHKLLAHGTTMLDVVYTNLSREVPFFLQQLKEKWFDNAVDHEKFLGLDLEYTTDQRGVAVIQLCFARHVLIFQWARSDKDCPELMDFLRSGITFAFVDIRNDKLKMRHSFGIEIPAGCLVDLQTVFMLRHDRTSMAHMAVALIDEGYGDMKTSFPKFQHKLWEKGPLDPINIEYAAKDAYISYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1Y4</accession>
    <name>A0A8I6Z1Y4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aberrant root formation protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407911</name>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0004560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="764" mass="87824" checksum="21D3461551EB4FF5" modified="2022-05-25" version="1">MATIYDIGLGAGFNILMATIFLLIFAFLRLQPINDRIFFPKWYLKGMRDSPSSAGAAVTKYVNLNVRSYLKFLSWMPAALKMPEEELIEHAGLDSVVYLRIYLTGLKIFVPITILAFAVLVPVNWTNDTLEGLKVVHSDIDKLSISNIPYGSKRFIAHLVMAYVFTFWTCYVLKNEYERVATMRLRFLASEKRRPDQFTVLVRNIPPDPDESVSELVEHFFLVNHPDHYLKHQTVYNANKLADLVEKKKKMRNWLDYYQNKLERKSKRPTTKTGFLGCFGSEVDAIDHCKSEIEKIGKEEVEERKKVMKDPKSIMPAAFVSFRSRWGAAVCAQTQQTSNPTLWLTEWAPEPRDVYWSNLSIPFVSLTVRRLIIGVAFFFLNFFYVIPIAFVQTLANLEGIEKALPFLKPLIEADTIKSFIQGFLPGLALKIFLIVLPTILMFMSQFEGLISQSSLERRTASKYFIFLFFNVFLGSIVTGSALEQLNTYLHASANDIPRIIGVAIPMKATFFITYVMVDGWTGVALEILRIRAFVLYHLKNFFLVKTEKDREEAMDPGSICFYWSEPRIQLYFLLGLVYAAVTPLLLPFILVFFALGYVVYRHQIINVYNQRYESGAQFWPNVHLRIIVALIASQLLLLGLLSTKGLEEATPVLLVLPILTFWFHKYCTHRYKPAFVRNPLQEAMRKDTLEHAREPNFDLKSYLADSYLHPVFKSDHVDKFYVADDPGAEEVIVATKRQSRRTTPVPSKYDGSDRFSVPDSIPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3B2</accession>
    <name>A0A8I7B3B2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123439612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972240.1">
        <property type="nucleotide sequence ID" value="XM_045116305.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I7B3B2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0058290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0058290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0058290"/>
    </dbReference>
    <dbReference type="GeneID" id="123439612"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0058290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0058290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0058290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439612"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055274">
        <property type="entry name" value="SWO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48429">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48429:SF1">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="1616"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="1705"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="827"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1214"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1530"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1769"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1875"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1918"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1953"/>
            <end position="2173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="857"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="941"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1569"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1769"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1877"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1976"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2002"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2024"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2043"/>
            <end position="2057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2130"/>
            <end position="2146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0058290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="2173" mass="231094" checksum="5022BE1F8A6648FB" modified="2022-05-25" version="1">MEYDDTDFQSQNFQLAGEDNSKPPSGLRPFALPKLDTDDQLQGHLRFGNLTDSEGFFSVGGHDNSWIEVLSTGSTVVDFSSSAAESCSISRSNYVWSEATSTECVEMLLKSVGENEMTGNMDDNVHQQLSGMDSQICPSNMQPKSSDSPSDSIVVPAENDQSQGTRSRMPEDPLTSQPQFEDIAPFSMVEKAEHAAASTLSGRKSNYMLDSVSEKCIVSEKLSSASQKTPEGCPAVDNYFKVVHDGDSLDNLNIHSAGVDSDKLSNEAFSELARIQNIYSTGSYHFEQGNHMHANKLEGMHELQKMTESSDGLLEAITNPVKMLQMNDDTCKSASDSLQPPLSEVEHAAEGHKISVDAHKKFLIEKFVTGEEPTSAKSCQNQSDLNNSNPRPFTPLSTESSELIQSPDGKQVAHVTEAPEETESDGVEDTIVDISKHEVPEQHQESVHDLKNVVMDDTSINNDVDSKLGVLEQHQDSVDNLQCVVLEEQSGRDEISAVSGNFECLVEAGHAGNDRGLTGTSKDEFESSGHVSPDNSSALLFDKNGPNVSSVNHEGPVKEDDTPVLEDKPGTNSGSQEKEIAPLINNLSSDIVCTTVADTSNKSMDKPDCSGGVPSDGSLAGVLEGNDSTISSINHVDSVEKGANSTSEVGGHNVASVPESLAKKSAMSVNSDINSISSSGTDPVAKNPQCEGLATSLGSLTTNQNQDKSGDHPDAHTENCQVDRPPLQSEDQEISHPQKCQIGGSSVQSGHHGNLATASSLDVSSGKGAQTIIETPLNAKDDLNVHIKDNEGSCNDGTCGSPTVISCTEPCLQEGGQEGMSVLHSNLTEQSEDKKDPTASAGAFPSSEDCSARNIKHTLTSAETNTTGDDRSFSFEVGNPPKVSEKVHRPAWSPFPRSKAALSTEVNSEVPKPGTPGNVLKHTSEVSKKTSVLETGKEQQSGSKVVESVGVLSSSSHIGHSTETKSAPLEQPQQHPTPEISALAHQPFTDLQHVQLRAQIFVYGALIQGIPPAEAYMVSAFGEPVGGGKPAWEAVWRVAVKIFQNLKSPVAGLETPTSSRIGSHVAEKASKGTAGKTSPTSKKGGKTVLPAHTAVTLHSPTFNMSPLGSSTLNLQRGSHLDFSQAVSPVFAYNSQTRQPTSAVASWFPQSPGPRAAPWLAPPQNLIFDSSMQPTVPSSDSAKGSSISISQAITPGLFLPTQASSTVASPLAVVQEEKQKTPASKRNRAGAASPKPRKRKKASVSQEQQPDIASSQLNTDVASSQLKTDIGSVIPATEQTPGFTLSTRSPSNVLGSRLVPNASLITSVPNYLGGKGAEQRIIFSEQISGAVDQSMDQARGASMYSEEALRHSEGVWNQLSANSRSKLPAEVEQKLTSAAASASAAVSVAKAAAEAAKMASAAALQAKMMAEEALGSTKSANSLQKRDTGEVDVNNMASVSSLTPKSSWKIKDSTNAPGSTISVAREVARKRVEEASAAAKRAENLDAILKAAELAAEAVFKAGTLIGLGEPLPFTLSELLEAGPDGYWKSDRVKNTKAGNTSENVATEELEIPADKSGRKHDSRAKYGQAIQNLEPSSSVKSSQPDKTPSGNGIEDNPTAAPLNGNTNDTAPSIIWNGIGKGSLVEVFAAEGGSRAAWFSAKVLDINEDNACISYEAHGEGTGLPKEWVSLKQEGEKAPQIRLAHPATVSNLKGTRKRRRDTAQNYSWAVGDHVDAWIKNSWREGIISQNGELCDTKFVVQFSAGDSLVLDAWNLRPSLVWQDGKWTEWSRARERKDKSNKGDSPYEKRQRTAVSDPVPVVGEARPPSKDKKSTNTVVSDPVPTVGEARPPTKDKKSTNTVASDPVLTVGEARPPAKDKKSVNTVASDPVLTVGEARPFTKDKKSVNTVGKPDEPRPLALSDRDVLFNIGKGATEIKTTRRPGLQKEGTKVFGVPKPGKKKKFMDVSKHYVGDQADKISEGSAASRFAKHPVPQVPRPRESTLKLDQRAKRASDMRSRGLKSAKSQTTSTNSVPGEGPSSTAVPSSSALESTFAFAASTASSSNPVNITVENNNSTHATDLRTEDASIPESRLPATPTIPAIKKNPSAANRAKRKFVPSADSNVNRRVLKTPDVSAKTSSDSAEPRRSNRRIQPTSRLLEGLQSSLIASKITGEKVPRTNFRSATSASRGKAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7T4</accession>
    <name>A0A8I7B7T4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7T4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000059">
        <property type="entry name" value="ATPase 2 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="2">
        <location>
            <begin position="158"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="46682" checksum="A8ACF46332EAF15F" modified="2022-05-25" version="1">MATAEEMAELETVDEEEVLRRLATGLTAVEAPRRLRLHGPNVVARPHHEGSMFRVFLMTIFSLWGWHHVFPEYKTIFSLWGWHHVFPEYKTMVCAILNSMSWVTVLTTVVSLAVASADKRSYMLAAFIFLLATTSIACITAKLAVEYAKSPLEAKAYAPRAKVLRDGSWIDVHATNLVPGDIIFLKVGDIVPANARVLRFQKIDTMTCWAKRSIDCVHGFLIYYAWTVSCGQGTAVVIATGSGIPRCTLRLYPQRYARPGQLKEGIMVAGCFCFSLILVGTLAEVVLILLFRKHSPGGMLHNCHFMPLIGGIPMAMPVVIYLALALGSLRLCFLGIASRGTVALEDLASMDVMLFNLTEPIDAAVLSLLDDPEQARAGIEVLEHHERFFVALKLMFLTTYIDSNGAKWLINVAPAEQSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B861</accession>
    <name>A0A8I7B861_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123430899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970665.1">
        <property type="nucleotide sequence ID" value="XM_045114730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B861"/>
    <dbReference type="SMR" id="A0A8I7B861"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0198780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0198780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0198780"/>
    </dbReference>
    <dbReference type="GeneID" id="123430899"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0165140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0165140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0165140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0198780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0198780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0198780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430899"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000003">
        <property type="entry name" value="Beta-D-glucan exohydrolase isoenzyme ExoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000002">
        <property type="entry name" value="Glycosyl hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051915">
        <property type="entry name" value="Cellulose_Degrad_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620:SF94">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620">
        <property type="entry name" value="PERIPLASMIC BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="3">
        <location>
            <begin position="31"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 3 C-terminal" evidence="4">
        <location>
            <begin position="397"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0198780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="66977" checksum="7AC36A1D5A274350" modified="2022-05-25" version="1">MAAAQGEQRPLYKDPSAPVEARVRDLLGRMTLREKAGQMAQIERCVASPRAVAELGVGSILSGGGRPPCDRASPSDWADMVDDMQRLALSSRLAVPILYGIDAVHGHNNVVGATIFPHNVGLGASRDAELVRKIGEATALEVRATGIHWAFAPCVAVCRDSRWGRCYESYSEDPEIVRSFTTIVAGLQGQTPADHPHGYPFLHSVRENVLACAKHYVGDGGTHKGINEGNTICSLDDLEKIHMKPYPDCIAQGVATIMASHSQWNGEHLHASHHLLTDVLKGKLGFQGFVVSDWEGVDHLCEPRGFDYRHCIAQSVNAGMDMIMIPFRFEKFLEDLVFLVETGEIPLSRIDNAVERILRVKFISGVFEHPFSDPTLLDVIGCKEHRLLAREAVRKSLVLLKNGKNREDTFLPLAKNAKRILVTGTHADNIGYQCGGWTIAWNGDSGKITLGTSILEAIQESVEVETEIVYDECPIEATIEAGNFSYAVVVVGEDPYSESVGDRTDLSIPFNGSDLITRVASKVPTLVILISGRPLVIEPQVLEKVDALVAAWLPGSEGTGVADCLFGDHDFVGTLPVTWFRYDDQLPINIGDANYDPLFPFGYGLKCSKAMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9Y9</accession>
    <name>A0A8I7B9Y9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9Y9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0515280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="5">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="141" mass="16488" checksum="4B68E557856E3BCA" modified="2022-05-25" version="1">MSLPCSDQSIRPRKMQSASLPRGVEAVRCWCGDVCKVKEVTDFSDWLGVKFFMCANYESDPPESISAYVRPPSPPALCMYYRWIDTEMLDWAVTEIRERGRRAWASLDLEERREKAEQRRKQSRRKSGKITVWSTGLFLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BD51</accession>
    <name>A0A8I7BD51_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIZ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0681820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF4">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="125" mass="14254" checksum="E2DD85103B8127BE" modified="2022-05-25" version="1">MSSSTSATRPSWTQYGPLSLERCPDCPRNAPLIWLTSKEVKNGNYGREFVKCESKPEGQIVKKCTRFEWLDDYVKRIQFNGAPTRELNLPLATMNLVFESVALIFGDADLKGEINKMNKNLKHMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-02" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D2KZ39</accession>
    <name>D2KZ39_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tonoplast intrinsic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HvTIP2;1</name>
        <name evidence="13" type="synonym">LOC123402410</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2010-01" db="EMBL/GenBank/DDBJ databases">
            <title>Barley Aquaporin (Tonoplast intrinsic protein, HvTIP2;1).</title>
            <authorList>
                <person name="Ligaba A."/>
                <person name="Sugimoto G."/>
                <person name="Horie T."/>
                <person name="Shibaaka M."/>
                <person name="Katsuhara M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. May be involved in transport from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AB540222">
        <property type="protein sequence ID" value="BAI66435.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D2KZ39"/>
    <dbReference type="SMR" id="D2KZ39"/>
    <dbReference type="STRING" id="112509.D2KZ39"/>
    <dbReference type="PaxDb" id="4513-MLOC_66094.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0498140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0498140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0498140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0600910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600910"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_4_1"/>
    <dbReference type="OMA" id="HINPAMS"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="D2KZ39">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF37">
        <property type="entry name" value="AQUAPORIN TIP2-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAI66435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="249" mass="25205" checksum="8EEA64D01FECA37D" modified="2010-03-02" version="1">MVKLAFGSCGDSFSATSIRAYVAEFIATLLFVFAGVGSAIAYGKLTEDGALDPAGLVAIAIAHAFALFVGVAIAANISGGHLNPAVTFGLAVGGHITILTGIFYWVAQLLGSAAACFLLKFVTHGKAIPTHAVAAGMNEFEGVVMEIVITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVAAGNFAGNWVYWVGPLIGGGLAGFVYGDVFIASYQPVADQDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F2CWR0</accession>
    <name>F2CWR0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Formate dehydrogenase, mitochondrial</fullName>
            <shortName evidence="5">FDH</shortName>
            <ecNumber evidence="5">1.17.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NAD-dependent formate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123407279</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. Involved in the cell stress response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>formate + NAD(+) = CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:15985"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.9" evidence="5"/>
    <dbReference type="EMBL" id="AK356063">
        <property type="protein sequence ID" value="BAJ87281.1"/>
        <property type="molecule type" value="mRNA"/>
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    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="63"/>
            <end position="359"/>
        </location>
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        <location>
            <begin position="155"/>
            <end position="333"/>
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        <location>
            <position position="121"/>
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        <ligand>
            <name>substrate</name>
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            <name>substrate</name>
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        <location>
            <position position="146"/>
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        <ligand>
            <name>NAD(+)</name>
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        <location>
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            <name>NAD(+)</name>
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    <feature type="binding site" evidence="5">
        <location>
            <begin position="255"/>
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            <name>NAD(+)</name>
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    <feature type="site" description="Important for catalytic activity" evidence="5">
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            <dbReference type="EMBL" id="BAJ87281.1"/>
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            <dbReference type="EMBL" id="BAJ95739.1"/>
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        <source>
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    <sequence length="377" mass="41492" checksum="D5981D5B8E5DB394" modified="2011-05-31" version="1">MAAMWRAAARQLVDRAVGSRAAHTSAGSKKIVGVFYQAGEYADKNPNFVGCVEGALGIRDWLESKGHHYIVTDDKEGLNSELEKHIEDMHVLITTPFHPAYVTAERIKKAKNLELLLTAGIGSDHIDLPAAAAAGLTVAEVTGSNTVSVAEDELMRILILLRNFLPGYQQVVKGEWNVAGIAHRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLQINPELEKEIGAKFEEDLDAMLPKCDVVVINTPLTEKTRGMFNKEKIAKMKKGVIIVNNARGAIMDTQAVADACSSGHIAGYGGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKDMLDRYFKGEEFPVENYIVKEGELASQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXI3</accession>
    <name>F2CXI3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 13">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123445381</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 13"/>
    <dbReference type="EMBL" id="AK356336">
        <property type="protein sequence ID" value="BAJ87554.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978294.1">
        <property type="nucleotide sequence ID" value="XM_045122359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXI3"/>
    <dbReference type="PaxDb" id="4513-MLOC_62418.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073290"/>
    </dbReference>
    <dbReference type="GeneID" id="123445381"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445381"/>
    <dbReference type="eggNOG" id="KOG3187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034302_2_2_1"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CXI3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF35">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
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    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0276">Fatty acid metabolism</keyword>
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    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ87554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="218" mass="24626" checksum="D020C647789DF7C6" modified="2011-05-31" version="1">MARPSRLYLLAYNSLQAIGWSVAFLRLLACLAPPVSVDSAYAAAGDLICLLQTCAVLETIHAAVGLVPTSPFLAFLQWGGRTHFVLALLRQIPEVQDSPSVFITFMAWSISEVIRYSHYALSTIKVCPAWLTYLRYTAFIPLYPIGVGPGEMWTMYQALPFVKERDLYSGFFAKFSMSYHSFLVGVLLCYPLLWLKLYLHVFKQRKSKLGKADRKKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2D189</accession>
    <name>F2D189_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357646">
        <property type="protein sequence ID" value="BAJ88860.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK360997">
        <property type="protein sequence ID" value="BAJ92204.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D189"/>
    <dbReference type="SMR" id="F2D189"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059430"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein 1"/>
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    </dbReference>
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        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein 1"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="hnRNP"/>
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    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
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    <dbReference type="PANTHER" id="PTHR48035">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48035:SF2">
        <property type="entry name" value="RNA-BINDING REGION RNP-1 DOMAIN-CONTAINING PROTEIN"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="363" mass="40082" checksum="6026C318D6052FF2" modified="2011-05-31" version="1">MASKLVVLGIPWDVDTEGLKEYMSKFGSLDDCIVMKDRSSGRSRGFGYVTFSSVEDAKNVVNSEHILGDRTLEVKIATPKEEMRASGNKKATRIFVARIPQSVDESMFRRHFQSFGEITDLYMPKELGSKEHRGIGFVTFRSAECVDNVMQENHEIDGTTVVVDRATPKDEEVRHPPRRGGAEREGGYGSYNAYITAATRYAALGVPTLYDHPGPAYGRGYLNEPPVSSKKIFVGRLPQEANTDDLWDYFGRFGRIVDAYIPKDPKKSGHRGFGFVTFAEDGVADRVARRSHEILGQEVAVDSAAPVEGGPSRGGYMEPPGPYGAYGPMMSYGQFSGSLGYDYGYGPSGSSGRSRVQPRYRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DD06</accession>
    <name>F2DD06_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK361773">
        <property type="protein sequence ID" value="BAJ92977.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962619.1">
        <property type="nucleotide sequence ID" value="XM_045106684.1"/>
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    <dbReference type="AlphaFoldDB" id="F2DD06"/>
    <dbReference type="SMR" id="F2DD06"/>
    <dbReference type="FunCoup" id="F2DD06">
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    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_56230.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073130"/>
    </dbReference>
    <dbReference type="GeneID" id="123414845"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059090"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073130.1"/>
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    </dbReference>
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        <property type="entry name" value="CHP01589_pln"/>
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        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871">
        <property type="entry name" value="OS02G0137100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871:SF48">
        <property type="entry name" value="OS05G0462000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09713">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ92977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="100" mass="11435" checksum="3EB6C3E9B242181B" modified="2011-05-31" version="1">MGDSSSSSASYIRMVHHLIEKCICFNLNKEGCMEALEKHAKINPVVTSTVWKELEKENKEFFESYNKDRVERNIEAETMERIQKMLTDAAASKSSDDDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJW8</accession>
    <name>F2DJW8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123427049</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK364186">
        <property type="protein sequence ID" value="BAJ95389.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966921.1">
        <property type="nucleotide sequence ID" value="XM_045110986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJW8"/>
    <dbReference type="SMR" id="F2DJW8"/>
    <dbReference type="FunCoup" id="F2DJW8">
        <property type="interactions" value="2874"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2DJW8"/>
    <dbReference type="PaxDb" id="4513-MLOC_20591.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176670"/>
    </dbReference>
    <dbReference type="GeneID" id="123427049"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0146120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0146120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0146120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0176670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427049"/>
    <dbReference type="eggNOG" id="KOG0687">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031814_0_0_1"/>
    <dbReference type="OMA" id="HEMRDAN"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="26S proteasome regulatory subunit N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF7">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="192"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075096"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ95389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="388" mass="44068" checksum="93B3D314655F8A33" modified="2011-05-31" version="1">MDGGGEEGKQQPQLVLAHKLFLLSQPDVDDLAKVGLRDDVLAAVKSDDMAALYESLAADGVLEMDAALLAEMRGRIEEETRKFDEKIADAEENLGESEVREAHLAKSLYFIRVGEKEKALEQLKVTEGKTVAVGQKMDLVFYTLQIGLFHMDFDLISKSVDKAKILFEEGGDWERKNRLKVYEGLYCMATRNFKKATSLFLDSVSTFTTYELFPYDTFIFYTVLTSVITLDRVSLKQKVVDAPEILAVIGKVPHLSEFLNSLYDCQYKSFFVAFSGLTEQIKLDRYLQPHFRYFMREVRTVVYSQFLESYKSVTMEAMAAAFGVTVDFIDQELSRFIAAGKLHCKIDKVAGVLETNRPDARNAFYQSTIKQGDFLLNRIQKLSRVIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTB2</accession>
    <name>F2DTB2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123400115</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Flower</tissue>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK367130">
        <property type="protein sequence ID" value="BAJ98333.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK376814">
        <property type="protein sequence ID" value="BAK08008.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950461.1">
        <property type="nucleotide sequence ID" value="XM_045094526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTB2"/>
    <dbReference type="SMR" id="F2DTB2"/>
    <dbReference type="STRING" id="112509.F2DTB2"/>
    <dbReference type="PaxDb" id="4513-MLOC_4948.5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499580"/>
    </dbReference>
    <dbReference type="GeneID" id="123400115"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0414620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0414620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0414620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0499580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123400115"/>
    <dbReference type="eggNOG" id="KOG1643">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DTB2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080022">
        <property type="term" value="P:primary root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ98333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK08008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="302" mass="32300" checksum="A88C69F347909997" modified="2011-05-31" version="1">MAAPSSLASSHLSRLAAAGAPAPQPHHQNHQLRLGCSRRRAQRLVAMAGSGKFFVGGNWKCNGTKESISKLVSDLNAATLESDVDVVVAPPFIYIDQVKSSLTDRIEVSAQNTWIGKGGAFTGEISAEQLVDIGCQWVILGHSERRHVIGEDDQFIGKKAAYALSQNLKVMACIGELLEEREAGKTFDVCFKQMKAFADNITDWKNVVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKTNVSADVASTVRIIYGGSVNAANCAELAKKEDIDGFLVGGASLKGPDFATICNSVTSKKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWE7</accession>
    <name>F2DWE7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430989</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368215">
        <property type="protein sequence ID" value="BAJ99418.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970751.1">
        <property type="nucleotide sequence ID" value="XM_045114816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWE7"/>
    <dbReference type="SMR" id="F2DWE7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0042770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0042770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0042770"/>
    </dbReference>
    <dbReference type="GeneID" id="123430989"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0033180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0033180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0033180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0042770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0042770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0042770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430989"/>
    <dbReference type="HOGENOM" id="CLU_069208_1_1_1"/>
    <dbReference type="OrthoDB" id="5594417at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ99418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0042770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="212" mass="23413" checksum="360CBB890FE5E285" modified="2011-05-31" version="1">MNPFAKKPTPREAIRNSKRELTNATRGIERDIGTLQQEEKRLVAEIKRTAKTGNEAATKILARQLIRLRQKISTLQGSRAQIRGIATHTQAMQANTSVSAGMQSASKAMGAMNKQMDPAKQMKVMQEFQKQSAQMDMTNEMMSDSIDNVLDDDQAEDETEELANQVLDEIGIDVASQLSSAPKGRIAGKKVEADESSELEELEKRLAALKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXY5</accession>
    <name>F2DXY5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123443143</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368753">
        <property type="protein sequence ID" value="BAJ99956.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975357.1">
        <property type="nucleotide sequence ID" value="XM_045119422.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975358.1">
        <property type="nucleotide sequence ID" value="XM_045119423.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975359.1">
        <property type="nucleotide sequence ID" value="XM_045119424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245450"/>
    </dbReference>
    <dbReference type="GeneID" id="123443143"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0203760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0203760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0203760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0245450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443143"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007402">
        <property type="entry name" value="DUF455"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782:SF4">
        <property type="entry name" value="DUF455 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782">
        <property type="entry name" value="SI:CH73-314G15.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04305">
        <property type="entry name" value="DUF455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ99956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="39923" checksum="29300E38F2C97320" modified="2011-05-31" version="1">MVPLRRLPTLPLAHGSTRRRLLPAGASAASPLPWPGLHAWRRAPPSDLRSWGPSGPCAPNADKTDAPEASHGSSLAEMGALVLSTADPLAKAHLTHAAFSRWASGGLPVGLARAPEHPARPEKPLAVTQKEVPTHKAMGVPLNAYMLHNLAHVELNAIDLAWDTVVRFSPLRDTLGDGFFSDFARVADDESRHFRWYSQRLAELGFRYGDMPVHNLLWRECAKSSSDVSARMAVIPLVQEARGLDAGPRLVQKLIGFADHRSADIVAKVAEEELAHVSVGLYWFLKVCEMMGRVPGATFRDLIKEHDVVMRGPFNYQSRDEAGIPREWYDETLKPEVASNLSEVHDRLACVVEMEKENASLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2DYN5</accession>
    <name>F2DYN5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123409966</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369004">
        <property type="protein sequence ID" value="BAK00207.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958770.1">
        <property type="nucleotide sequence ID" value="XM_045102835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DYN5"/>
    <dbReference type="SMR" id="F2DYN5"/>
    <dbReference type="STRING" id="112509.F2DYN5"/>
    <dbReference type="PaxDb" id="4513-MLOC_72166.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667500"/>
    </dbReference>
    <dbReference type="GeneID" id="123409966"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0553830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0553830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0553830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0667500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409966"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044915_0_0_1"/>
    <dbReference type="OMA" id="MWRKIDI"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DYN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905761">
        <property type="term" value="F:SCF ubiquitin ligase complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000037">
        <property type="entry name" value="F-box protein SKIP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF33">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="35"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK00207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="310" mass="35414" checksum="D0FE4C0DBA79EBF5" modified="2011-05-31" version="1">MAAPSSPSCLLEEPRRLPPPPEDGMDTEEVVVEESRDWAELPRDVLLAVLSRLDHIEILMGPDMVCSPWRRAAMDEPELWRRIDMRFHYADDFYLTSRKFHQMVRAAMRRSAGRCEAFWGGAYVAETNLSLVGDAAPSLKSLRLIECRDMVDLAFKPVITMFPMLEELELSNCMHRFPITLEVIGDACPLLKRFRLSQGSFYSERVDDSAAMAIAMMPELRSLQLTADSLTNSGLELVLNGCPHLESLDIRSCYHVCMDDDMQAKCSRIKTLRHPEDSMDDYDLPFSYTIPRPCWSTTPIEYAWSCQSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F2E147</accession>
    <name>F2E147_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123397479</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DVL/RTFL small polypeptides family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369868">
        <property type="protein sequence ID" value="BAK01069.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044947943.1">
        <property type="nucleotide sequence ID" value="XM_045092008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E147"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441320"/>
    </dbReference>
    <dbReference type="GeneID" id="123397479"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0365330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0365330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0365330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397479"/>
    <dbReference type="OMA" id="FTRRCAT"/>
    <dbReference type="OrthoDB" id="784420at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012552">
        <property type="entry name" value="DVL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051525">
        <property type="entry name" value="DVL_RTFL_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33102">
        <property type="entry name" value="DVL19-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08137">
        <property type="entry name" value="DVL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK01069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8447" checksum="64FC1CFF4283FB7A" modified="2011-05-31" version="1">MEMFMDDKWKLSRKGSRRSAAVAPAAATGSPVGVKGRTSRGSGRSVPGRLASLAKQQRARFYIMRRCVTMLVCWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2EAB8</accession>
    <name>F2EAB8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123426438</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Flower</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK373093">
        <property type="protein sequence ID" value="BAK04290.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966215.1">
        <property type="nucleotide sequence ID" value="XM_045110280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EAB8"/>
    <dbReference type="SMR" id="F2EAB8"/>
    <dbReference type="PaxDb" id="4513-MLOC_36414.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0025720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0025720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0025720"/>
    </dbReference>
    <dbReference type="GeneID" id="123426438"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0025720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0025720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0025720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426438"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EAB8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000006">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000570">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000740">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="4">
        <location>
            <begin position="41"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK04290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0025720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="773" mass="85369" checksum="3C964A37ABE9F631" modified="2011-05-31" version="1">MGTSGSKGMDGVGGLGSAALAGGGGDGMGGDEGGGAVTGSWHGGAQLYVSLKMENARIIGDLVPHVYGSEPIIGSWEPARALAMERELASMWELSFVVPPDHETLDFKFLLKPKDSETPCIIEEGPTRLLTGGMLEGDVRLANFRLNGDDELLEFRVFNKADIVSPLDLAASWRVYKENFQPSKVRGIPDISINEAPTHATEDGSGSSLELDLEHYVVPAPTAPPTEYAANLAATPASLDQPEAPWTNDMLLSDGIQSPRSAAADFKDDSYHNKDTEASVADSSNSKKLQVSGMVESKSVGTLIQLQKKDKPQGLYVDRGVVSPKLGKSFSACSLASGLNFGSTKAMPEAAGAVAAAAVADRLHGSKEDRKLAIVLVGLPARGKTFTAVKLTRYLRWLGHETKHFNVGKYRRLKHGANQSADFFRPDNQEGIEARNEVAALAMEDMIDWMHGGGQVGIFDATNSTRKRRYMLMKMAEGNCKIIFLETICNDRNIIERNVRLKIQQSPDYADQPDYEAGLQDFLERLTNYEKVYEPVQEGSYIKMIDMVKGEGGQLQVNNISGYLPGRIVFFLVNSHLAPRPILLTRHGESLHNVRGRVGGDTVLSEDGELYSKKLANFIEKRLKNEKTATIWTSTLQRTILTATPIVGFPKIQWRALDEINSGVCDGMTYEEIKKIMPEEYESRKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYSYFADRPLREVPDMEMPLHTIIEIQMGVTGVEEKRYKLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECK4</accession>
    <name>F2ECK4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402762</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK373879">
        <property type="protein sequence ID" value="BAK05076.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952653.1">
        <property type="nucleotide sequence ID" value="XM_045096718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECK4"/>
    <dbReference type="SMR" id="F2ECK4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0563940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0563940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0563940"/>
    </dbReference>
    <dbReference type="GeneID" id="123402762"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0467500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0467500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0467500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0563940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0563940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0563940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402762"/>
    <dbReference type="HOGENOM" id="CLU_014546_7_2_1"/>
    <dbReference type="OrthoDB" id="683650at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK05076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0563940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="453" mass="48333" checksum="48987860DA672DE4" modified="2011-05-31" version="1">MPPMVRTRSTTAVPVHAAGGGELPDPGRVVPLSPFDAFWVSLPPVRRVFLFRSLPGVPFSDVVGTLRSSLAQVLPAFHPFAGSLTYSPDSRAVSIVLPDEWEGFPCGGVTFVEAEADLDFERLVEEAEEHDQDALGQLVPDIRRDQLPAPVMAAQVTEFVGSGGGVALGVAVHHTAADGRGIWRFLDMWAATAAGVEDGGVRAEPAPLHDRRLVQFHGDEELVRLFLLQVAPNLPTIAPKQDPVLDTRRRLSRRTFTFAASAVQRLKQRLASAANIGTAPSTFAALAAHGWVSIARASGFADDAPVYVVFLADCRAYMSPPVPNAYAGNCVALCTASLSGSVLSGPDGPARALLAVREAVAEAKRDPLRDRARWLAKFMAIPPGRAVVLVGSPWFPAYGLDFGFGRPARVELESMNNDGEMVLVAGREAGSVQASVAIAAGKMLAFRDKFMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F2EF40</accession>
    <name>F2EF40_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123412387</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Seed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK374766">
        <property type="protein sequence ID" value="BAK05962.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961266.1">
        <property type="nucleotide sequence ID" value="XM_045105331.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961267.1">
        <property type="nucleotide sequence ID" value="XM_045105332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EF40"/>
    <dbReference type="SMR" id="F2EF40"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726520"/>
    </dbReference>
    <dbReference type="GeneID" id="123412387"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0602550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0602550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0602550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412387"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000280">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000441">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF358">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="303"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="650"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK05962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726520.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="742" mass="82298" checksum="236F72763F7E6C90" modified="2011-05-31" version="1">MRRAPWPTPGSVRQATELHALVTTSGRLLHPPSAAHLLNSLTSCISPSDPLHLRYALSLFDRMPCSTFLFDTALRACFRASSGPDRPIILYRRMHGVDVPPDAFTFHFLFKCCARGGAHVLLGRMLHAACFRTLLPSAVPLIASPIIHMYAELGLPGDARRAFDEASVKDVVAWTTVISGLAKMGLLDDARRLLARAPVRNVVTWTGLISGYSRAGRAAEAVDCFNSMLSDGIAPDEVTVIGMLSACGQLKDLNFGCSLHMLVGDKRMLMSDKLVVALIDMYAKCGDTGRAREVFDALGRGRGPQPWNAMIDGYCKVGHVDIARSLFDQMEDHDVITFNSLITGYIHGGRLREALLLFTKMRRHGLGADNFTMVGLLTASASLGALPQGRALHACIEQRLVERDVYLGTALLDMYMKCGRVEEAMVAFKQMSVRDVHTWSAMIGGLAFNGMGKAALEHFFWMKCDGFHANSVTYIAVLTACSHSCLLDEGRLYFDEMRLLHNIRPQIEHYGCMIDLLGRSGLLDEAMDLVQTMPMQPNAVIWASILSACRVHKNVDLAQNAAHHLLKLEPAEDAVYVQMYNIYIDSRQWEDASKIRRLMEKRGVKKTAGYSSIAVAGQVHKFIVGDRSHPQIAEIVVMMEEIGRRLKSAGYSPITSQITVDVDEEEKEQALLAHSEKLAIAFGLVSLAPNLPVHIIKNLRVCEDCHSAIKLISRLWNREIIVRDRSRFHHFRGGVCSCNDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKY5</accession>
    <name>F2EKY5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123407526</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Flower</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK376813">
        <property type="protein sequence ID" value="BAK08007.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956615.1">
        <property type="nucleotide sequence ID" value="XM_045100680.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956616.1">
        <property type="nucleotide sequence ID" value="XM_045100681.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956617.1">
        <property type="nucleotide sequence ID" value="XM_045100682.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956618.1">
        <property type="nucleotide sequence ID" value="XM_045100683.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956619.1">
        <property type="nucleotide sequence ID" value="XM_045100684.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956621.1">
        <property type="nucleotide sequence ID" value="XM_045100686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKY5"/>
    <dbReference type="SMR" id="F2EKY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0672900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0672900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0672900"/>
    </dbReference>
    <dbReference type="GeneID" id="123407526"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0558390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0558390.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0558390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0672900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0672900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0672900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407526"/>
    <dbReference type="HOGENOM" id="CLU_768571_0_0_1"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF14">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="246"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK08007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0672900.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="41595" checksum="02C6B04D47CE2C05" modified="2011-05-31" version="1">MSRPLARRAVPPFLRLRSTACDDGHWIGRLDHRDWLAPNEVLKIFASIRDAALITSVFRKACARRDYKPSEALYGLMIDRLAGARRFGDVEELLARARAERFRFSDDFFYRLIKMYGNVANHPEKAMETLYAMSEYGCWPSTKTFNYVLHMLVCRRQYEVVHEVYSSAPRLGVELDTCCFNILIKGLCQFGRFNEALSLLDEMPKQDCRPNAMTYSTLMHFLCRNCRVDEAFELFERMRKEEIDADTVVYNILVSGLCREGRVTSAYDLFKSMSSQGCHPNSGTYQVLLDGLVASKNFVEAKDLVGMMSAESLRPSFSSYKLLIDGFCSVNCLDDAHHVLKQMVDQGFVPRMSTWTKLLTSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>M0W7G2</accession>
    <name>M0W7G2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123442561</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044974578.1">
        <property type="nucleotide sequence ID" value="XM_045118643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W7G2"/>
    <dbReference type="SMR" id="M0W7G2"/>
    <dbReference type="FunCoup" id="M0W7G2">
        <property type="interactions" value="683"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W7G2"/>
    <dbReference type="PaxDb" id="4513-MLOC_44513.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0065070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0065070.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0065070"/>
    </dbReference>
    <dbReference type="GeneID" id="123442561"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0051890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0051890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0051890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0065070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0065070.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0065070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442561"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W7G2"/>
    <dbReference type="OrthoDB" id="122279at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W7G2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000287">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000492">
        <property type="entry name" value="probable receptor-like protein kinase At1g11050"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043891">
        <property type="entry name" value="SPARK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF62">
        <property type="entry name" value="OS05G0423500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19160">
        <property type="entry name" value="SPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="312"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0065070.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="643" mass="68432" checksum="A1F32D2854ABDEDE" modified="2013-04-03" version="1">MPTPRLLRILPLLLALLLAGSPDGAAAAGGGNGTCPLDLSYVPTFPWDPTPCAGAAPNMTACCQTLLSVLGIGLAGRLRATGQFRVPSAGASAACLADFAEAVAAAPASLPGASLVQSCFPSPDEFTASPSFCAGVTTAAEYRAVVGNASVAGLDAACGTDLSSLPICDRCQSAGIVATSPLIAASANATADSPRNCFYLTVMYAAGISSGEGPISPTAAHCILGLGLSTPPSHHSKSNNAAIYATTIPIAFILVLSVLAFFVWRKTRHAKNKKRNHTIPEEGSEGRRPHLRPNTGSILFNIAELSKGTDNFADRNLIGRGGFGVVYRGVLADGSVVAVKKMLNPEMDGGDEEFTNEVEIISHLRHRNLVPLRGCCIVDDDIEEGKQMFLVYDFMPNGSLEEFIFQDRVGGSKRAALTWTQRRTIIMDVAKGLEYLHYGVKPAIYHRDIKATNILLDNEMRARVADFGLARRSREGQSHLTTRVAGTHGYLAPEYALYGQLTEKSDVYSFGVLVLEILSGRHVLDMTASTGPVLITDWAWTLIKAGQSREVLDDALSTCESPRGEAIERFVLVGILCAHVMVALRPTITEAVKMLEGDMDIPEIPDRPLPFGHNFLMFSEAGSNFSASPAISGPLMDNGDMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0WCF3</accession>
    <name>M0WCF3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123445570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978504.1">
        <property type="nucleotide sequence ID" value="XM_045122569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WCF3"/>
    <dbReference type="SMR" id="M0WCF3"/>
    <dbReference type="PaxDb" id="4513-MLOC_4848.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0323330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0323330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0323330"/>
    </dbReference>
    <dbReference type="GeneID" id="123445570"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0269860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0269860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0269860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0323330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0323330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0323330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445570"/>
    <dbReference type="eggNOG" id="KOG0149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WCF3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12384">
        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF21">
        <property type="entry name" value="OS01G0958500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0323330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="313" mass="32600" checksum="FBE8FBC4BDDACAD8" modified="2013-04-03" version="1">MSMAHQAMAAGGGDTRMTKVFVGGLAWETSKEGVRGHFERFGEILEAVVIADKGTGRSKGYGFVTFREAEAAMRACLDPYPVIDGRRANCNLACLGVQRSKAPQPLPYLQPYAAVGPVHGGGNINTRAMKAAIAGGGGGGASFVDHGIQQGIPAAAYNMYGYSPYFSDYGYQPLTYYQAYGGLAGGAQYQVFNGGATTAPPAGLAMADPTGLYPYCQYAPSVNAAAAYSMMQYPQMYQYAAVGAPPDSSPTAVSGLHQFIGAAAFEPSTGNQAGGMTLAQLTAPALPASAPQYQYRLISPMPIATPDQKKPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>M0X6R8</accession>
    <name>M0X6R8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell cycle checkpoint control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1 2">Belongs to the rad9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981514.1">
        <property type="nucleotide sequence ID" value="XM_045125579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0X6R8"/>
    <dbReference type="SMR" id="M0X6R8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0375490.1">
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    </dbReference>
    <dbReference type="GeneID" id="123448627"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0312530.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0375490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0375490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0375490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448627"/>
    <dbReference type="OrthoDB" id="60092at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000012">
        <property type="entry name" value="cell cycle checkpoint control protein RAD9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026584">
        <property type="entry name" value="Rad9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04139">
        <property type="entry name" value="Rad9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009303">
        <property type="entry name" value="Cell_cycle_RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="406"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0375490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="48769" checksum="C82F17C46DEF7E8B" modified="2013-04-03" version="1">MELSMSGGALRTFSRCVTCLARIGSDLVFQAYPDKLELHTLNSSRSAFASVSLTRDFFDHFHLPADLHAAPSSTPLQCSVLLKSVLAVLRTPTSAIDRLNASLPNPQASKLEFVIHCLSGRKKTHRISCSAESDVQTLALDRNRFPSRLAIRPRDLTRLLSNFQSSLQELTVIATEPAAVLPGVDDGTEGKAVELLSYIDPTKDDYDSRLHTQLWINPAEEFLEYEHAGDPVDVTFGVKELKAFLTFCEGCEVDILLFYEKAGAPVLLAPRFGYDDGSSSNFDVTLVLATMLVSQLNDGSVPQQPATSAHHAEEPRAAAAAPSPVPQNVSNHTRIWSELSGVTPKSFEANAENCAQKERNGIANVLNDTSVLPNTNAPCKLPVTDNANHMEQPVQMDHFEEPPEVVVDNPRSQHHPSNWVGADEDNDDEEDEELFVQTTPHYMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>M0XYY2</accession>
    <name>M0XYY2_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123411591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960492.1">
        <property type="nucleotide sequence ID" value="XM_045104557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XYY2"/>
    <dbReference type="PaxDb" id="4513-MLOC_64328.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683500"/>
    </dbReference>
    <dbReference type="GeneID" id="123411591"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0567430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0567430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0567430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0683500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411591"/>
    <dbReference type="eggNOG" id="ENOG502QR8G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="HGTRPSN"/>
    <dbReference type="OrthoDB" id="1925372at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XYY2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35729:SF2">
        <property type="entry name" value="OS08G0528800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35729">
        <property type="entry name" value="T1B9.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="548" mass="58384" checksum="C741EFA3F69046FA" modified="2013-04-03" version="1">MGVLGDRSGWCFCSGGGAKLERIKSTLLATKGPALAAVSFPRGGGEGEGGGGVGGGGGGGKCGGGFLIHRALLLTTHGTVPSAAVAGAAEVQLSHGRLLARLVPQRFFITSPILDLTVVGLDVVDDGSDSHGQQPHFLKTCLNPSLDLGSTVLLLGHTNKRDLAVGEGKVVIATDNLIKFSTDEVSWHPGSAGFDMHGNLAFMVCDPMKLAPSTPTGYASASSIALLASKMDVPTQFGIPIPAVCEWLKQHWSGSLEDVSKPMMPPARLISAGQRSEPSSQSHLHYIKTTEREGGALSSSQMPARPTQRHGSCSSASAKISYGENDSVNSHSFQGQRDPASQMPRPKEQPGSVMDISFPPGHPRSIFLPLSLKQMMPAENKIKPNCSVSDESRVANAQINCDAMHNVAYQENCWNEAQSSSSPPAISERDKRDGFSSGEETMYSAETMESRNIPSPKDKRPQIVGRSQSFANHSKWDSPKSVESSKGPPSRSHAFIPLRKPHLQAASISQKSQVYFSPTVSSNMKKRNLSQIPMKPRQSAQVTSKWIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>M0YR50</accession>
    <name>M0YR50_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123442778</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974847.1">
        <property type="nucleotide sequence ID" value="XM_045118912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YR50"/>
    <dbReference type="SMR" id="M0YR50"/>
    <dbReference type="PaxDb" id="4513-MLOC_7196.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232910"/>
    </dbReference>
    <dbReference type="GeneID" id="123442778"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442778"/>
    <dbReference type="eggNOG" id="KOG1196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="YIRDGTI"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YR50">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF71">
        <property type="entry name" value="2-ALKENAL REDUCTASE (NADP(+)-DEPENDENT)-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="40"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="353" mass="38265" checksum="172991CBB829A430" modified="2013-04-03" version="1">MAELKSRRVVLKDYVEGYPTEAHMELLPAAPVDEAEDGSVLVKNLYLSCDPYMRPKMSRPLHQSYTAAFVPGAPITGYGVSEVVRSSTPGVVAGDLVWGMTGWEDYSVIKPPFTAMLTKIQPDDGVPLSYYTGVLGMPGLTAYVGFHHIGSAKPGDAVFVSAASGAVGQLVGQFARLMGCRVVGSAGSKEKVDLLINKFGFHDAFNYKEEDGDLAGALKKRFPDGIDVYFENVGGKMLEAVLLNMKVHGRIAVCGLISQYNLTAGEKEADVGVRNMTSLVAKRVRMQGFIEPDHKHLYPEYRAWVMPHIKEGRVVYVEDVADGLEAAPGALIGLFHGRNVGKQVVKLTTPDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>M0YXS5</accession>
    <name>M0YXS5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123451785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044984262.1">
        <property type="nucleotide sequence ID" value="XM_045128327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YXS5"/>
    <dbReference type="SMR" id="M0YXS5"/>
    <dbReference type="PaxDb" id="4513-MLOC_74184.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091990"/>
    </dbReference>
    <dbReference type="GeneID" id="123451785"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0075730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0075730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0075730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0091990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451785"/>
    <dbReference type="eggNOG" id="KOG0724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="GANEEHR"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YXS5">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044636">
        <property type="entry name" value="RADIALIS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF85">
        <property type="entry name" value="OS05G0579600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="96" mass="10763" checksum="EB002E553E52AD7E" modified="2013-04-03" version="1">MASLSMSVGWTPKQNKLFEQALAVHDRDTPDRWHNIARAVGGGKSADDVRRYYELLVHDIARIEAGKVPFPAYRPPCPGPGHNASYEADRLKHLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GSU9</accession>
    <name>A0A287GSU9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123421397</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044963470.1">
        <property type="nucleotide sequence ID" value="XM_045107535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287GSU9"/>
    <dbReference type="SMR" id="A0A287GSU9"/>
    <dbReference type="FunCoup" id="A0A287GSU9">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093680.7">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093680.7"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093680"/>
    </dbReference>
    <dbReference type="GeneID" id="123421397"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0093680.7">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093680.7"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093680"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287GSU9"/>
    <dbReference type="OMA" id="ISMDANP"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287GSU9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000008">
        <property type="entry name" value="Drought-induced S-like ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF47">
        <property type="entry name" value="OS09G0537700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093680.7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="245" mass="27649" checksum="A32D5AE4ABF411DC" modified="2017-11-22" version="1">MAMRAISLCLILGLLAAAAPASATSFDFYYLILMWPGAYCADSDYGCCVPKYGYPAEDFFVESFMTFDLSLNKAIVRCNSDKPFDVNKLEPIENNLNHYWSNIHCPRNDGTGTWKSEWRSYGVCSGLKLVDYFRAALNLRKNADVLGALAEQGINPDYRLYNTEHIEWAVNQKLGVMPGVQCRDGPFGKKQLYQIYLCVDKDGKTFIDCPKLPKLHCPEEVLFHPFHTWMLNATSSAKITLPTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287HVD7</accession>
    <name>A0A287HVD7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosyl-DNA phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123425840</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965518.1">
        <property type="nucleotide sequence ID" value="XM_045109583.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A287HVD7"/>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
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        <property type="term" value="F:exonuclease activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="Tdp1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
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        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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    </evidence>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139330.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="75094" checksum="2684E1FD916ACCCB" modified="2017-11-22" version="1">MSSSSRVRVGTLVPFVEGKSGSPNASSLPMPSIPIFEGSNVVGRSNLVAVDKRVSRKHLSLRAVPDGSVEVVVEGTNPIVVRSEGQRRKVCAQQRAKIMPDDVLELIPGEYFMKYVNMSDERKISASVDSHDLKKGKRHSEEDSVAAKRNRQVMEDEALARTLQESFAEESASVTEVLSSLDSAGSSERNKERTHSVGPLKDVLPLSFRLMRVQGLPSWTNTSTVTIQDVIQGEVLLAVLSNYMVDMDWLLTACPSLRKVPHVLVLHGEDGASLERLKKTKPTNWILHKPPLPISFGTHHSKAMLLVYPQGIRVVVHTANLIHVDWNNKSQGLWAQDFPWKEANDMSTNIGFENDLVDYLRALKWPEFRVNLPVVGDVNINAAFFRKFDYSSSTVRLIGSVPGYHVGPNMKKWGHMKLRSVLEECVFEKQFCKSPLIYQFSSLGSLDEKWMSEFACSLSAGKADDGSQLGIGKPLIVWPTVEDVRCSIEGYAAGSCIPSPQKNVEKDFLRKYWSRWKADHVGRCRAMPHIKTFTRYNGQNIAWFLLTSSNLSKAAWGALQKNNTQLMIRSYELGVLFLPKTLQSVPQFSCTDKSRSNLDKLALGKNIKTKLVTLCWKGDEEKDPSAEVVRLPVPYQLPPQLYGPEDVPWSWDRRYTKKDVYGSVWPRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287JYX6</accession>
    <name>A0A287JYX6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123442451</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
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    <dbReference type="PaxDb" id="4513-MLOC_62179.1"/>
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        <property type="component" value="Chromosome 3H"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:defense response to bacterium"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:innate immune response-activating signaling pathway"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
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    </dbReference>
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        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF999">
        <property type="entry name" value="NB-ARC DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="168"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="432"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="557"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="940" mass="106242" checksum="B6F79B3789D7E9DF" modified="2017-11-22" version="1">MEAVVASAITGAMRTLLPKLGVLLESKYKLSKAMKKEIASLSDEMSSMSALLVKLAETDDGELDVQDKVWRDQVRELSYEMEDCVDTFTDDLDGGGGLWRGLTTLKMRYNITRWIAELKSRMVEVNKRHSRYKLDERACSISRSPVTIDPRLYALHTEAASLVGIDGPKEKLIDLLLREEKYGSNHRALKVVAITGFGGIGKTTLASQVRDMIKGRFDCTAFVSVSQNPSMVDILSTILSQVGSSVPVPSGQNSVQSLISKLREHLMAKRYLIIIDDIWTIEPWNTIKCAFVENKRASRVITTTRIEDVAQACCCGFRGHVYKVKPLNVLDSRRLFHRRIFSSEDACPEKLKDVSDEILNKCEGVPLVIVSVASILASHKEGKSKKFWEKIQNYFGFQLEGNNALQWLRHVLNLGYIDLPLDLRTCMLYLGIFPEDSEIMKDDLEKRWIAEGFVPERHGDGPEAIAEDFFNELINKNMIQIAEFDDCGDVLSCRVHDVMLDFIVWKSMEENFIDIIKGPPTVGDPQCMKGCLQVRRLSLQVRKSQHNELLGSMALTQARSFNFWGSAQWMPSLSRFQLLRVLHLDLGVYDSRNEEELTSIGSLTQLRYLRIRGAVFKKLKKQLQGLKHLKTLEIVGEDIEKILHLELSVTKLPATLWHLIVPYTVKLPGQINKMPNLRTLGEISIDIQDVENIKGLGELGDLRDLKIVLDKGDREGHCADLVPSLSSLKCLESLTIRMAGSLKIVDVLTCWSPPSHHLRRLHVLGLPFSTVHEDWISQLDNLRSLKIHVVSLPRDGVDVLARLSLLVHLTLHVKKNVPEEGLVVRAASFPNLKDFVFRCEEICLVFEAGAMHKLQRLAVDCYAKAERQGGDLLEGIQHLESILSFEFYIYEWNDLTVPQYGLQAYCSPQPKNWNVESLEAALRKAINKHPGTPDVRIQSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WH57</accession>
    <name>A0A287WH57_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123409897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958707.1">
        <property type="nucleotide sequence ID" value="XM_045102772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287WH57"/>
    <dbReference type="FunCoup" id="A0A287WH57">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_66325.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681260"/>
    </dbReference>
    <dbReference type="GeneID" id="123409897"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0565580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0565580.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0565580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0681260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409897"/>
    <dbReference type="eggNOG" id="ENOG502R3HR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287WH57"/>
    <dbReference type="OMA" id="FRRWPQH"/>
    <dbReference type="OrthoDB" id="691075at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287WH57">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35510">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35510:SF2">
        <property type="entry name" value="OS08G0520600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="244" mass="25555" checksum="E68D11F7391F027A" modified="2017-11-22" version="1">MVVLMEYAPGVGVTTKRKGEEEPGLFAFPDDGDSGSVPISCRATKMRRLESAGTGHDVPAAAAGVGMEGDVMMAEELPAPSPSLGAAEGGKMAVVVYDHRAVDAARRGGRRGLLGQWRLRPWAPLSAGADWIRDMLREADGRTVRAVLSSAQEEGGVDLALVPWGAAHVSAEANQASTAAETVDGEEDAEGTAAMDVEEVGEHHQTQGGGAGCGEGYLRQWPQHCLAPPPLPAVRQARPAVWSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W785</accession>
    <name>A0A8I6W785_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123414765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962614.1">
        <property type="nucleotide sequence ID" value="XM_045106679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W785"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0072920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0072920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0072920"/>
    </dbReference>
    <dbReference type="GeneID" id="123414765"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0072920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0072920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0072920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123414765"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053063">
        <property type="entry name" value="PWWP_domain_containing_PDP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42851">
        <property type="entry name" value="ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42851:SF25">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0072920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="741" mass="81736" checksum="380F8F0C42766B17" modified="2022-05-25" version="1">MIWLSGVEGGHGGTNPEAGADELPPVDAATDGEAMAQGSFGEADFDAMMVESGGLQTAEISNPAVPQHDAEAESNEDDERARYHLPPLDRDGFQVSDLVWAKLEGHPWWPGEIFDPSDASELALKHQKEGHHLVAFYGDGSFAWCHESQFKPFMENRAEMEKQCNSDAFAIAVNGALQELSRRILAAASCSCLPEELSENGMSYIGENAGLKTGFTCSKVNQAEILKYFSPEHLLHYMKLLALSPCQGGVLQDLVIACSQLMSFYRSKGYAEIASFQSGSAWAENNIDTTVTNEVQPSHVKPKRGRGRPRKRKPDDNVELTEGRPIVKPQNGPAVHNNIGETECGEFDISMKKPAKRRRVQRRYDVDPKDLSGMAASTEMGMIEKTEGDGSEEWKMLPCPKEEITDHMQDAYWSGLSLHTVSTQSLKGASGKTRPIRRRRATRRTCAPSSDLSSPVQNMHPETLDVNRKIEVIKRSIIHVDEKMLHDVNPTALVFCFGKSIALPSKMDFIRMFSRYGPLKETETETHKNTNTVKVVFRKRVDAERAFSAAGNFGSFGPSLRSFRLVNMPFSLRTTELSYPMLRPEDSSMEIPVCRVSGVALDYAQIDIFDKADKGEAVKNPSVEHTEMVKLAGQGEGNTECALHAGGRNEESPDVLSGTMQTEIADEAPRHCLDCVDGIVKASEDTMEVDNTDEAREETSIPHAFTGYVAKQSLGDEYTFEGIEGSTVEMPVGETRKFSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WBF3</accession>
    <name>A0A8I6WBF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123425776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965433.1">
        <property type="nucleotide sequence ID" value="XM_045109498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WBF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128260"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128260.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128260.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128260"/>
    </dbReference>
    <dbReference type="GeneID" id="123425776"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128260.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128260.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425776"/>
    <dbReference type="OrthoDB" id="673639at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF340">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128260.2.CDS2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="330" mass="37776" checksum="F0A2528E487D68ED" modified="2022-05-25" version="1">MTRRRCRRRRSARRPSPPAALEDDDLLRKIFLLLPPQPSSIPRLSVVCKHWRDVVTDPQFLRVFRDQHRNPPLLGLVMGHTGSPYFRSDLDPPDRIPHERFFPPDILCMHMDLFDCRHGRVVFFDHRLREVMIFDPATGDRRHVVVPPVFNEKEIGVFNAAVVCVAGDEGHVHGDCHTSPFQVVLMGISDDYNQAFACVYSSESGIWGGIISIANREMYDLEQPSTLIGNALYWLFPGDHEEGILEFDLGKQSLANIEMLQYLMYYKHRSLQVMVVDDGCVGLAILSCYRFEMWERKVSCDDGVAGWVLQTTVQLNTILGLGPMGEWTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCV5</accession>
    <name>A0A8I6WCV5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="13">2.7.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123436322</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 and participates in phytic acid biosynthesis in developing seeds. Phytic acid is the primary storage form of phosphorus in cereal grains and other plant seeds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13253"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.159"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-inositol 1,3,4,6-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20940"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.159"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 15">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044971558.1">
        <property type="nucleotide sequence ID" value="XM_045115623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCV5"/>
    <dbReference type="SMR" id="A0A8I6WCV5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053380"/>
    </dbReference>
    <dbReference type="GeneID" id="123436322"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0041780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0041780.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0041780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0053380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123436322"/>
    <dbReference type="OrthoDB" id="25308at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000056">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF24">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038186">
        <property type="entry name" value="ITPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol-tetrakisphosphate 1-kinase N-terminal" evidence="17">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="16">
        <location>
            <begin position="124"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR038186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038186-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="347" mass="38322" checksum="C7151813920A754A" modified="2022-05-25" version="1">MAAAAAAAAEASSADSRRYVVGYALAPKKQNSFIKPSLISRAAARGVDLVPVDDARPLADQGPFDLVIHKLYGHDWRAQLQAFSARYPSVPVVDPPHAIDRLHNRISMLQVVSELDVPPGDADRRDTFGIPSQVVVYDAAALADSGLLAALRFPLIAKPLVADGSAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGVIFKVYVVGGHVTCVKRRSLPDVSKEILEDAAAEGTISFSQVSNLPNQRTAEEYYEDMRLEDAVMPPTDFVNEIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFPGYAKMPGYEIVLTDFFWDMVHKDDVALKEEEEQSNHAVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WHD2</accession>
    <name>A0A8I6WHD2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092860.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="64702" checksum="10C667AA53D59087" modified="2022-05-25" version="1">MACRETGISRIRTGTSGGMTKDAAVAVHGEPTRGNTPLSVSFSMPGSPSGLHLAQARHRMLPSAHASIAEVHATPVLPSESEAEGRHPVVPQLLKHPQASVVIESAGEGPRSNSTRELDRQFDQFRTFSGRLNRQISNLRGLPQDPTGTDVEHGAVSKLLEEDTDVPTADRYFAALEGPEFDTLRSTEVAVLPKDKPWPFLLRFPITAFGMCLGVSSQAMLWKTLQSEPSTAFLHVHPAVNHVLWWVSVALTGIVSITYLLKVVFYFEAVRREFHHPVRVNFFFAPWIVCLFLVKGVPHPVWKIHHVVWYVLMAPILWLDLKIYGQWMSSGERRLSRVANPSNHLAIVGNFVGALLGARMGLRELPIFFFAVGLAHYIVLFVTLYQRLPTNVQLPKDLHPVFFLFVAAPSVASMAWTRISGEFNDGAKLLYYISLFLFMSLVARINLFRGFRFSLAWWAYTFPMTSVALATVLYASEVDNLVTRAMALGLSGIAVMTVIAMLATTMYHAFVCGDLFPNDVSIAITKQRPKFSKILTHLQTSGSDIKELVLSIPNFSSNSKHGVYSDDSDSNARTSNDDSVRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="7" type="primary">LOC123430457</name>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Potential calcium sensor.</text>
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    </dbReference>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0177660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="26426" checksum="3F41955E4EE4DDC9" modified="2022-05-25" version="1">MVQGKGFAPPPPNLSPPNQLNSSDLQSLHSKATPPPSMPPSGLLSYIPTSLSSILPARGCGAAPSPSPPPPASPAPSKMSPSVKAADRAELARVFELFDRNGDGRITREELEDSLGKLGIPVPGDELAAMIARIDADGDGCVDVEEFGELYRTIMSTGSGGGQKGSSDAEAEEEDEDEDMREAFRVFDANGDGFITVDELSAVLASLGLKQGRSAEECRRMIGQVDRDGDGRVDFHEFRQMMRGGGLAALA</sequence>
</entry>
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            <fullName evidence="2">DUF1771 domain-containing protein</fullName>
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    </gene>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045111663.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190240"/>
    </dbReference>
    <dbReference type="GeneID" id="123427583"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0157610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0157610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0157610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0190240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427583"/>
    <dbReference type="OrthoDB" id="1928104at2759"/>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="entry name" value="At5g58720/SDE5-like_UBA-like"/>
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        <property type="entry name" value="DUF1771"/>
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        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR SDE5-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47872:SF1">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR SDE5-RELATED"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24767">
        <property type="entry name" value="UBA_At5g58720"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="338"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="491" mass="54708" checksum="386AAEC89159A54F" modified="2022-05-25" version="1">MDLSSSLASTSDEETRALNALLDAFSCAFSLEDIANAYCRANGDVNKAGDFLTDLQLSMPQSNEVDSSVETNLPQFGKAVEENSMDNSNRTRNLSCVGKAAEESSVENSSQTRTREKSQKSSASFGTVSSMLGKGSARATTAPANTAPGKGKPLKVELPEYMRDDLKTDESDSAPKRETLNNRDVEEFLFSMLGEGFKLNMEVIRDVLGSCGYDMKKSMDELMSFSTNDLGKRPENEHIAVQDTAVESSFSMGSCLGSQSTSRSQITPGELLESIFTAPERSEEEPKVRRYELGANRRRVPDQKPVVKPLEDISPPSTDLPVKIILGSKEPVVRDEDDYQNYRKAAKQHWDMMKQYYAKAVDAFREGNKKEAEYLRTEGKNYYMMARLSAEKSAGEITKSKQESKNELCLDLRSQDAANVANLLRLHLRQLANIPSFENLRVIIGVDDGTFKMGQRRRKVEKFLEKKSVEWTEDEANPGTILIPINQVKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXX8</accession>
    <name>A0A8I6WXX8_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0182270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0182270.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0182270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218930"/>
    </dbReference>
    <dbReference type="OMA" id="RQSCERQ"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035286575" evidence="1">
        <location>
            <begin position="28"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="14743" checksum="CB2EA14BD95AAE3C" modified="2022-05-25" version="1">MAPAAAGGASLLAAVAVFSMLIMSSQGNPRPLCSDCEPQCRTNCTAEVETTCREYCTRGGGPRESCRSSVFEQCTANGDCSGSNGTSTCDCNTVAENTCLSVTDGTLQCTPCMSNIYDQCFHPCTNDCNNRCKKKGCHHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZF5</accession>
    <name>A0A8I6WZF5_HORVV</name>
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        <recommendedName>
            <fullName evidence="11">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123446175</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978791.1">
        <property type="nucleotide sequence ID" value="XM_045122856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZF5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0348370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0348370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0348370"/>
    </dbReference>
    <dbReference type="GeneID" id="123446175"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0290630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0290630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0290630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0348370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0348370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0348370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446175"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000003">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000024">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF39">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="454"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0348370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="727" mass="82332" checksum="8A22F28811530F78" modified="2022-05-25" version="1">MAGFLQRHVLPPFRRPPLPFFHHGAGAASSSNQPQRRRPWTPSRILDPGDDVVLNWNRLFLVTCMVGLFVDPMYFYLLYAKGQSCVKMNMGIGVAVTAVRTVADLFYLAHMILKFRTAFVAPSSRVFGRGELVRDPDQIAIRYLKNDFIIDLAAMLPIPQIIIWFIIPAVSTTSANHTNNTLSMIVLIQYIPRVFLIISLNSKIVKSSGVVTRTAWAGAAYNLLLYTLASHVLGALWYLLSIERQYTCWVDQCTSDNGTDPRVPICSMSYLDCKSLEDPLRMKWHAASNITKQCGLPGAKFEYGLFEDALKLDIVDASFFEKYLYCLWWGFRNLSSYGQNLQNSTYAGETIFCILICIMGLVFFSHLIGNMQTYLQSMTVRLEEWRVKRRDIEEWMRHRQLPLELQERVRRFFQYKWLATRGVDEESILQSLPLDLRREIQRHLCLALVRRVPFFSQMDEQLLDAICERLVSSLSTKDAYIVREGDPVSEMLFIIRGELESSTTDGGRTNFFSSITLRPGDFCGEELLTWALMPNPSLNFPQSTRTVRSVTEVEAFALRAEDLKYVANQFKRLHSKRLQHAFRYYSHQWRSWGACFVQGAWRRYKKRKLAKELMKQEGILYDGQGDAGDDDMQGGAGAIAGADASTPLLGEYKDSAGAASSSGDGGGDGGTHLGVTFLASKFAKNTKKGAHQKGGAQQRIDDVSTMKFPKLAKPDEPDFSLHTEDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0C2</accession>
    <name>A0A8I6X0C2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0C2"/>
    <dbReference type="SMR" id="A0A8I6X0C2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0018820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0018820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0018820"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0014620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0014620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0018820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0018820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0018820"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="OS05G0315700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0018820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="421" mass="45089" checksum="B00D13A62715602F" modified="2022-05-25" version="1">MGIALKTHEAPGLQNRARYTRTLAAMALPFAVRRREPVLVVPASRTPQETKRLSDIDSKESLRVLMPGVFVYRGRPACGDDDPVDIIGRALSEALVHYYPLAGRFREVEGGKLVVDCTGEGVLFAEADADVRLTELEEAAGGSQGLTLPFPCMDELLLDMDCSSGGVLTWPLVVIQVTRLLCGGFVFGMRINHAMCDAAGVYLFMSAVADLARGLPAPAVAPSWSRGHLDAPHTKPDVAPSLPFPSHGSDDTVMRSFVFRLADIALLKKRLPGAPAATRRNAALRLPTGYYGNACVPLRAAASADDMGGSSLGDVVEMIQKTKTTAAHVRSAADMLALHGPPPFDSPNMFLLSDLRHVGFHRVDFGWGEPVYGGPSRVYLHGTFFVAVKDGDGEDAVAVPITLPRTAMGRFAAEIETSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0Y2</accession>
    <name>A0A8I6X0Y2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
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    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0Y2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0258860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="5">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="146" mass="17274" checksum="60BE593DFE92C64E" modified="2022-05-25" version="1">MSLPCSDQSIRPRKMQSASLPRGVEAVQCWCGDVCKVKEVTDFSDWLGMKFFMCANYESDPPESISAYVRPPSPPPLCMYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYYVEQRAFLDEMKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2F6</accession>
    <name>A0A8I6X2F6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SIAH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123439299</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045116037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2F6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228050.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123439299"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0189140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0189140"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SINL_plant"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_SIAH"/>
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    <dbReference type="PANTHER" id="PTHR46632">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632:SF18">
        <property type="entry name" value="OS01G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="126"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="317" mass="32702" checksum="95B49E6C97DB7283" modified="2022-05-25" version="1">MQVAAGCKGSPVATETEMGDPQSAEEPRLAAEPLLHAPMKQEIAVHQAAGDSGAVVAADHGSRMEIAVKMDVTLLHCALCFSPLKPPVFQCKGGHVACGRCVAEPCAECGGAFDARNAAMDAVVSSTTVDCDHAGCGRYVTYHDLAGHRGECPHAPCACTAPGCAFAGPAPALLGHLTTLHSMPVHRVQYGKVLVLQVPASQEPRGLLLGDEDGRAFLIVGGALGPGAAVSAVCVRAEAMVWPRYTLKVWASGPAPAPNRKGKADTVMAEIEVTSSTAPGAVAVEELAYLAVPPKLLVGAGASRRMSLKIRIDKFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3X7</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228850.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="291" mass="32503" checksum="43A7568C37336F92" modified="2022-05-25" version="1">MALLAARRAAAALVRPSAAAAILRHTPAGARFSSAPDYRPFVRGLREGSEFLRAEKEQSSRPSAAELRAKADSLRAATHDGEIVGVLNSVIQSHRRDLVKGIPKDFPFEMSRTDGIPNITLTRSLNGEQIEVLVSMPKLDQDEDCDVGLQEDEAEISPEEYSIPVRVTVSKADGSSLEFTCTANPDNIVIETLFMRRNKSSGAEDAVASEGKEHDLGLLDDEGPHFHELDKNLQEAFHQYLEVRGITPMTAKLLREHMMSEDSHLSPKEKSDHRRRHLLWLMKLWDFVKKD</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I6X4J1"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0305210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="14567" checksum="3D8A10CE1D4AB0EA" modified="2022-05-25" version="1">MYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYCVEQMAFIDEMIRKNQEEKLRLEEVYRQREHAREAERERKRERARAAKALEEAGDGKGKYPRWTQ</sequence>
</entry>
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    <accession>A0A8I6X4R6</accession>
    <name>A0A8I6X4R6_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">SNF2 N-terminal domain-containing protein</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4R6"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0235020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235020.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNF2-rel_dom"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="112"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1048"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    </evidence>
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</entry>
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    <accession>A0A8I6X6U8</accession>
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        </recommendedName>
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    </gene>
    <organism evidence="11 12">
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Phragmoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045127036.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8I6X6U8"/>
    <dbReference type="SMR" id="A0A8I6X6U8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085490"/>
    </dbReference>
    <dbReference type="GeneID" id="123449719"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0070320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0070320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0070320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0085490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085490"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:phragmoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
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    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
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    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="31"/>
            <end position="299"/>
        </location>
    </feature>
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        <location>
            <begin position="517"/>
            <end position="609"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085490.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="68616" checksum="F613B60062353C7E" modified="2022-05-25" version="1">MENLISLVNKLQRACTALGDHGEESALPTLWDSLPSIAVVGGQSSGKSSVLESVVGKDFLPRGSGIVTRRPLVLQLHRIDGDREYAEFMHVPRKRFTDFAMVRKEIADETDRQTGHGKGISSVPIHLSIFSPYVVNLTLIDLPGLTKVAVEGQPESIVQEIENMVRSFIEKPNCIILAVSPANQDLATSDAIKISREVDPKGERTFGVLTKIDLMDKGTDAVDILEGRSYRLQFPWIGVVNRSQQDINKSVDMIAARRRERDYFANTPEYKHLAHRMGSEHLAKSLSKHLESVIKSRIPGLQSLITKTVAELETELSRLGKPIANDAGGKLYTIMEICRMFDGIYKEHLDGVRPGGEKIYHVFDNQFPVAIKRLQFDKQLSMENVRKLITEADGYQPHLIAPEQGYRRLIESCLVSIRGPAEAAVDTVHGILKELVHKAINETHELKQFPTLRVEVGNAAFESLERMRDESKKNTLKLVDMETSYLTVDFFRKLPQDVEKGGNPSHSIFDRYNDSYLRRIGTTVLAYVNMVSSTLRNSIPKSIVYCQVREAKRSLLDHFFTELGAREIRQLSKLLDEDPAVMERRTNLAKRLELYRSAQAEIDAVAWSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7W8</accession>
    <name>A0A8I6X7W8_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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        </location>
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
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        </location>
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            <end position="145"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="18641" checksum="A9B3B20DCB6FD850" modified="2022-05-25" version="1">MGNSLRCCLACMLPCGALDVVRVVHLSGHVDELTCPLIASDVLAAHPSHALTAAGSAGAARRIVIVSPDSELKRGRIYFLIPTACSAPAAELKRPKPGHGACESKTKKRHGHHDGHRKSGAAVAAAASTAEQDNYLRELLTEKRETSHRRRRSSSARSGVWRPRLESIAEEPSD</sequence>
</entry>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.3HG0216180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0260400.1">
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0260400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="77" mass="9012" checksum="7578D12C3231DC37" modified="2022-05-25" version="1">MRSQERQYICFLFCSRKKKKREEKKTRVCSSPFPGSPPPPQLGNQRRVVVVGTYGGRKPQSRTGRWAHRAGQERRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFQ8</accession>
    <name>A0A8I6XFQ8_HORVV</name>
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        <recommendedName>
            <fullName evidence="13">Ubiquinol oxidase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>2 a ubiquinol + O2 = 2 a ubiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30255"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="EC" id="1.10.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the alternative oxidase family.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A8I6XFQ8"/>
    <dbReference type="SMR" id="A0A8I6XFQ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213170"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBIQUINOL OXIDASE 4, CHLOROPLASTIC_CHROMOPLASTIC"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="18251" checksum="FB5C6A9170B06793" modified="2022-05-25" version="1">MNEFHHLLIMEALGGNSVWIDRFLARFSAFFYYFVTVAMYMLSPRMAYHFSECVERHAYSTYDKFLKLNGEELKKLPAPEVAVNYYMNEDLYMFDEFQTSRAPNSRRPKVDNLYDVFVNVRDDEAEHCKTMKACQTHETLRSPHSVQSSTEADSK</sequence>
</entry>
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            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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        <property type="nucleotide sequence ID" value="XM_045126464.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982401.1">
        <property type="nucleotide sequence ID" value="XM_045126466.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045126467.1"/>
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    <dbReference type="GeneID" id="123449302"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329680"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
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        <property type="entry name" value="PPR_rpt"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF11">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="320"/>
            <end position="354"/>
        </location>
    </feature>
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        <location>
            <begin position="387"/>
            <end position="421"/>
        </location>
    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="58137" checksum="858A8649E5851AFB" modified="2022-05-25" version="1">MPFRPTFPRRCLDDRKLSSFLSALASFSANPSPSPPAGSVPAARTPAAYNALMSAYSRAGRPDEVLRLFRSLPFPPTAPLFTTLISSLAVSGRPRAARAAFSSLLVSGLTPTASAFTALLKCHDCSLDSMHHIFLAMASASCSPDAAVYNCYISMLCDSGRLEEAWGILDHMMDGGVRPTVRSYTAILRGYCEQGKILEAERLFDAMVDAGCPPDVVSYSVLIEGLCGIREFHKVERILERSEEKGWTPNAVTYNIYMSALCRMGFLDEACHQVDKMRSRGLSPTVETLSILFDCLCRDSRFSEAVCLLEHSEELGWHADVFCYNTLMGRLCDAGDFARVFKLLVDLLKKGIGPDLFSFTIAIRGLCGLGKFRLAKCLIDNEWIGYDVVAFNTLIHGFYNAGDLRGVKRTYINMCSRQISPNNFTNATLIDSLCKDQKFVEAINSLASLRDGSVPDHVVHLNNRLVKGVRYTKVLNLLDEIHCRGFELDTCIFIPLVRVLCWEGYYKRENINEVSLILTSLGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XP33</accession>
    <name>A0A8I6XP33_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ASCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123395244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044946140.1">
        <property type="nucleotide sequence ID" value="XM_045090205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XP33"/>
    <dbReference type="SMR" id="A0A8I6XP33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537400"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537450"/>
    </dbReference>
    <dbReference type="GeneID" id="123395244"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0447190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0447190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0447190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0537400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0537450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395240"/>
    <dbReference type="KEGG" id="hvg:123395244"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.30:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF5">
        <property type="entry name" value="ASCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="535" mass="59073" checksum="2AE08C7D03C559DA" modified="2022-05-25" version="1">MRGGRSGGGGGLRNPCLTMHQPWASLLVHGIKRVEGRSWPSPVTGRLWIHAASKVPDPDTVAAMEDFYREIYAVDGVHHIDFPRHYPVSRLLGCVEVVGCVRSEELVCWEDVPQSVRLEGLTDFCWLCENPQKLVVPFEMRGYQGVYNLERRVYEGAARGLSPVQGPLPVKFPLPDPRNPLSLKPGSLNFESSKSVVVKTESVSAAIAGARAAATQYSRKGASAATSSEIQTRGKSRENHADSSSGSGTLPSAAQRRSPPHPQNHDPSPVVHNTPSHPQNQDPSPVVQNSSSHLVNQNPSPVIQNSPSYSQTRNSQYIAQSSPSYSQTRNPQYIAQSSPSYSQTRNPQYIAQSSPSYSQTRNPQYIVQSSPSYSQTQNPQYMVQSSPSYSQTQNPRYMVQSTPSYSQNQSQSSNIQSTPFYFHDQNQSPNAHNSPSYFHYQNQSSTVHNSPSYSHNQNALSNVQSRPSHLQNHNPSPVAHNSPSFLQHQNAEPRRSPRLQSGAPHRLVAVALRDLKQSSLRESGEQSSAPRSWPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPG8</accession>
    <name>A0A8I6XPG8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPG8"/>
    <dbReference type="SMR" id="A0A8I6XPG8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0539900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0539900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0539900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0539900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0539900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0539900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
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    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
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    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="29"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0539900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25031" checksum="6D36FBFEACE556DD" modified="2022-05-25" version="1">MPTSFQKVLAHGTTMLDVVYTNESREVSFFLEQLKEKWLDVAMDHEKFLGLDLEYTADQLGVAVIQLCFARHVLIFQWARSDKHCPELMDFLHSSITFASVDIRNDKLKMRHNFGIEIPTGCLIDLQTVFRLRHVRTSMAHMAVALIDEEYGDMKTSFPKCQHKLWEKGPLDPINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQF3</accession>
    <name>A0A8I6XQF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine-rich transmembrane CYSTM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950138.1">
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    </dbReference>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    <reference evidence="16" key="1">
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thought to be a Golgi-localized beta-glycan synthase that polymerize the backbones of noncellulosic polysaccharides (hemicelluloses) of plant cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like E subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045093581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTK4"/>
    <dbReference type="SMR" id="A0A8I6XTK4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0488950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0488950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0488950"/>
    </dbReference>
    <dbReference type="GeneID" id="123399156"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0405090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0405090.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0488950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0488950.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123399156"/>
    <dbReference type="OMA" id="CEENTSW"/>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016759">
        <property type="term" value="F:cellulose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000138">
        <property type="entry name" value="Cellulose synthase isolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cellulose synthase-like protein E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="672"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="110"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023316"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037405"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0488950.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="82332" checksum="D333B092AB1E1A6A" modified="2022-05-25" version="1">MERTSRLRLFETETRGGRAAYRLHAVTVAAGILLLIYYRATRVPAAGEGRAAWLGMLAAELWYAAYWAVTQSVRWSPLRRRPFRDRLAARYGESLPCVDIFVCTADPHSEPPSLVISTVLSLMAYNYPAGKISVYLSDDGGSILTFYALWEASIFAKHWIPFCKRYNIEPRSPAAYFSESDGHQDLCTPKERSLIREMYEDMTERIDTAVSSGDISEEIKANHKGFYEWGQENTSKNHQPIVQILTEGKNANDDEGNVLPTLVYMAREKRPQHHHNFKAGAMNALIRISSVISNSPIIMNVDCDMYSNNCDTIRDALCFFLDEEVGHKIGFVQFPQNYKNLTKNNIYGNSHQVTNQVLMGGMDSVGGPMYVGTGCFHRREILCGRRFTEDYKEDWNGGIKDKTQESIDKIEEKAKSLAASTYEHDTQWGDEVGIKYGYPAEDIVTGLEIHCRGWKSVHSNPPRPAFLGVAPTTLAQTLLQHKRWSEGSFSIFLSKYCPFMFGHGKIKLRHQMGYSIYGLWAPNSIPTLYYVIIPSLALLKGISLFPEITSPWITPFIYVLCVENMYSLYEALSCGDTLKGWWNEQRMWMVRRITSYLYGLTDTVRKLLGLSKMTFAVTSKVSEENESKRYEQEIMEFGLSAPEYVIIATVALLNLICLVGGLSQIMTGGWNVLLNVFSPQIILCGMLVITNIPFYEAMFVRKDKGRIPFSVTLASISFVILALLVPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU17</accession>
    <name>A0A8I6XU17_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0264010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="14490" checksum="5361BD3A784E8CF7" modified="2022-05-25" version="1">MYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKEECEDYCVEQRAFIDEMKRKNQEEKLQLEEVYRQREQAREAERERKRERDRAAKAAEEAGDGKGKYPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU51</accession>
    <name>A0A8I6XU51_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XU51"/>
    <dbReference type="SMR" id="A0A8I6XU51"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0314070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314070"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Wall-associated receptor kinase 3"/>
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        <property type="entry name" value="Wall-associated receptor kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035266335" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="31"/>
            <end position="748"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
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        <location>
            <begin position="362"/>
            <end position="409"/>
        </location>
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    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="475"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="82315" checksum="E661106019F40A8C" modified="2022-05-25" version="1">MTITPSSQSHNSHRLPIFLVLLLHLASSSSRSLILEAAAAAEEEDEQRSITLPGCPERCGDTPIPFPFGTKPGCFREGFQVTCNDSFTPHRAFLAYTGVNQHIAEIYYKVGWHYPVWRIDHDNTALELIDISVTKGEARAYGLVSSLCNAANLSGDYVAKCQNLALGERGPFILSVTRNLLVGVGWRVEPKIMSYLWSAYRNSTYEFSLACLSDLMGMPKLLQLATNGSCSSRGCCQAALPEAAPLTDFGTWFSIDENPMWQTNPCSYAMVVESEWYSFSTPDLYGVEVLPRRYPRGVPFVLDFSIRNGACPGKGQKPPRDYACVSGNSYCANASGGAAHGYVCRCLEHYDGNPYIANGCQDIDECRLRKLNPELYPCSTDGICKNRLGGYDCPCKPGMKGDGIKGTCTDKFPLAARVIVGGICGIFSIAAISFLILLRKEKQKTREFYQKNGGLTLEKAKFIKLFKKEDLKPILKSSNLIGKGGFGEVYKGFLDNEEVAVKKPISGTMLENEQFANEVIIQSRVIHKNIIRLIGCCLEVDTPMLVYEFLSNGSLYDILHSKKKVSLTLGVRLSIVVESADGLVYMHSKTNTKIIHGDVKPANILLDDKFAPKISDFGISRLIARDKKHTGSIIGDMSYMDPVYVQTGLLTDKSDVYSFGVVILELISGKKATHSDGSSLVNNFLETGKKKNKATELFDKEIAVAGDLDILDSLAGLAVECLNLDVDQRPSMTEVLERLLIINQSHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XW39</accession>
    <name>A0A8I6XW39_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XW39"/>
    <dbReference type="SMR" id="A0A8I6XW39"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0200900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0200900.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0200900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0241920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000227">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="14">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="124"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="47563" checksum="CBF864773A91D65F" modified="2022-05-25" version="1">MAECSEACLSNCSCFAYAYAGQGCLVWTDRLLNAKLGQSNGGTTAASDGETLYLRLAASEMTRSARSNRRRRAIIGVAIAAGTAAAAAVLVMVALMMRRSRTNNVVQDGGLVAFRYRDLRSATKNFSEKIGEGGFGSVFKGQLRDTDIAVKRLDGSFQGEKQFRAGVSSIGVVRHVNLVKLIGFCCDGDGRFLVYEHMPNRSLNIHLFGQSDGGGGVFLDWSTRYQIAVGVARGLAYLHEGCRDRIIHCDVKPQNILLDVSLRPKIADFGMAKFVGRDFSRALTTMRGTMGYLEPEWISGTPITPKVDVYSYGMVLLELVSGRRNSSGGSWTWTTHADDDQVVDYFPVRASRKLAAGEVRSLLDESLCGEAELKEVERACKVACWCIQEDEAVRPAMGQVVQILEGVLDREMPPLPRLLEAIFAGRPRSVDTSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZ57</accession>
    <name>A0A8I6XZ57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC123406072</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044955499.1">
        <property type="nucleotide sequence ID" value="XM_045099564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZ57"/>
    <dbReference type="SMR" id="A0A8I6XZ57"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0560970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0560970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0560970"/>
    </dbReference>
    <dbReference type="GeneID" id="123406072"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0465980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0465980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0465980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0560970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0560970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0560970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406072"/>
    <dbReference type="OrthoDB" id="647974at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000403">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF18">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035305821" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="32"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="682"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="762"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="791"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0560970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1050" mass="114486" checksum="27322FA59DD0B457" modified="2022-05-25" version="1">MQMQPHRCPDSRPLPLLLLLLLASLASPASSCTEQERSSLLRLLAGFSRDDGLAAAWRRDTDCCSWEGIACAGPDGAVTDVSLASRGLEGSISPSSLGNLTGLLHLNLSRNSLSGGLPLELVSSSSIVVLDVSFNLLTGGLSDLPPSTPARPLQVLNISSNLFTGRFPSAIWEGMKSLVALNASTNSLTGRIPTAPCVSAPSLAVLDLSFNDFSGNIPPGLSNCSALKLFGAGYNNLVGTLPDGLFKVTSLEHLSLPSNGLEGAINGIGKLTNLVTIDLGGNELSGSIPESIGDLKRLEELHLEHNNMSGELPSALSNCTNLVTIDLKSNNFSGELTKVNFTGLPNLKVLDLLYNNLTGTVPESIYSCSRLTALRLSSNNFHGQLSERIGNLKSLSFLSLAKNNLTDITRTLQILRSCKSLTTLLLGFNFMHEAMPQDVNTDGFDNLQVFSMNDCSLSGTIPHWLSKLPNLGMLFLHNNHLTGSIPDWISSLNLLFYLDITNNRLAGEIPSDLMEMPMLKSDKTAPKVFELPVYNKSPYRQYLMPSAFPKVLNLGINNFTGMIPQKIGQLKGLIFLNLSSNQLSGEIPEAICNLTSLQTLDLSGNHLIGKIPAALSNLHFLSKFNISNNDLEGSIPAVGQLSTFPNSSFDGNPKLCGHMLVNHCSSAKTPPITQRRKKKKGVFALAFGVFFGGVAILFLLARLLFLLRGTSFLTKRWSNNNNDIEATSSNLNSEYSLVMVPRGKGEQNKLTVADLLKATKNFHKDHIIGCGGYGLVYKAELPDGSKVAIKKLNSEMCLMEREFSAEVDALSMAQHDNLVPFWGYCIQGDSRLLIYSYMENGSLDDWLHNRDTDDNPFLDWPIRLKIAQGASQGLSYIHDGCKPHIVHRDIKSSNILLDKDFKAYVADFGLSRLIFPNRTHVTTELVGTLGYIPPEYGQGWVATLRGDMYSFGVVLLELVTGRRPVHICPRSKELVKWVQEMRSKGEQIEVLDPALRGTGYEEQMLKVVEVACQCVNHNPGMRPAIQEVVAFLNSIDANLQRQNSASSQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZP5</accession>
    <name>A0A8I6XZP5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123448735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981661.1">
        <property type="nucleotide sequence ID" value="XM_045125726.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981662.1">
        <property type="nucleotide sequence ID" value="XM_045125727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZP5"/>
    <dbReference type="SMR" id="A0A8I6XZP5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379380"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379380.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379380.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379380"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379380.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379380.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379380"/>
    </dbReference>
    <dbReference type="GeneID" id="123448735"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0315630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0315630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0315630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379380.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379380.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379380.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379380.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF80">
        <property type="entry name" value="HTH MYB-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="228"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="357"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="387"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379380.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="451" mass="49480" checksum="F6FBF5C81F5620C1" modified="2022-05-25" version="1">MRRCDLRQCHNSRVSGGMSSSLPILPNSLKETFHGPYNPQLTPMQRQLTSDFVPLYQSAFPSATLHPRAGAMRSSYSASLGYSANPLDSVPNHERQSMVAPFAPQSSDIEVFQALSNNIPGGHTEATWFPGSADSLSDYRDNIPASGSQIQNSGPAVTSDVVAKQNEWWADIMNDDWRDILDATAADPQSKSMVQPSNSAASQPAVNQPASSHGGEICNVASPPNGNSAAKQRMRWTPELHECFVDSVNKLGGSEKATPKGVLKLMKVDGLTIYHVKSHLQKYRTARYKPDVTEGTADKRTTTEELTLDLKSSMDLTEALRLQMEVQKRLHEQLETQRKLQLRIEEQGKYLQMMFEKQSKSNTEKGQDLSSGATTTLSSDPSHSANRNRDNDAADDLHRTGENPVSAEIGETSMHAGGNREMAEIESSDPLANTNDGSKAPQEKCRRVHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2F3</accession>
    <name>A0A8I6Y2F3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123440963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973437.1">
        <property type="nucleotide sequence ID" value="XM_045117502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2F3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321150"/>
    </dbReference>
    <dbReference type="GeneID" id="123440963"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268080.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440963"/>
    <dbReference type="OrthoDB" id="696443at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34998:SF10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34998">
        <property type="entry name" value="OS04G0357400 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035193841" evidence="1">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="119" mass="12498" checksum="D9363EB0553747F6" modified="2022-05-25" version="1">MDRKVRIKLVILAVVLAGPDTTRAAALAGGTDSIDAAGAVRPLMSTSSSSWMKLEDGVAPELSAVTALDLDVQREVLRGIDAGAGALGPDRQVCLKKKKCPAPGEPYTRPCAKIYHCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3W1</accession>
    <name>A0A8I6Y3W1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40557" checksum="306055F24C3DF0A4" modified="2022-05-25" version="1">MAEVGDRFCVMELRSGRLVRRSPPPRRARSRRGPCGGADDRLSALPDDMLLLVLARTGILSSRWRGLWAHLPDLTFRDVAPAEIEAARLLDAVVRFSPRELRFSYKSETNMRLPDLHLPCFPRATSIDIDSDSNICFTQLRSGEFSALERLSLKCGIVIDFDTLIARCPCLRVLSVFITRVSDIVISVHSVSLQILEFKCNDAYTNSIDIVTPKLKQLELIFNTGKDLSVSISAPMVEKVVWIRELPFEDLNPLFGFWRLDLMAVLPADRFGDGDYGLELGISTAVDESYYELDLTHEMEKLPVIDFSVLALSLTTDGHVFGALLWRLLGFHQIRAATKRLEVVLYSVILLLLSPDTHTH</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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                <person name="Mayer K.F."/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I6Y5J4"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0433140.1"/>
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    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0433140.1">
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_GRF"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0433140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="15968" checksum="5D489DC28C2BE43B" modified="2022-05-25" version="1">MTRCTDCPRIAPLKRLTSKEEKNGNFGREFVKCESKPEGQIVKKCHHFEWMDDYIQRLQGLGLLDSRGDAIREFNLPHDSAAPAAAARPEYPTVVDVELNTELKKMNKNFKQLIELKKQSNLIALGILALGFFYLMAISR</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <end position="75"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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    </evidence>
    <sequence length="98" mass="11035" checksum="324599C7FD3EE6D1" modified="2022-05-25" version="1">MEQCRQGKSAKRIRNFGKRIGSEDWARGSRPRTRRLLADCSSCSRGESGSPRAGRGTDRESPLRELSSSMKQSTQNWYRQGESDCLIKTKHCDGPRGC</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <strain evidence="10">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
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        <text evidence="1">May be involved in the transport of sterols.</text>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Oxysterol-binding protein-related protein 2A"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF168">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="40"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="733" mass="82744" checksum="5BE054552B69A853" modified="2022-05-25" version="1">MRMRARDLHPLCCLPVDCPGGWCADRSPPSPPPPPPAPAPAAVAGLLHKWTNIGKGWRPRWFAILRGGLLAYSKIRPRAAASPPSPPAPEDGGPRLIGPAGFAAPAPEDRPIGLVHLKISSFRESKSDDKRFYIITPTKTLQLRTNSVKDRVAWIEALVSARSESSTNGGLLYDQNDASFSTDRLRNRMHAEGLGEEVIKDCEQIVHSEFLQYYTQMRRRCEEYLSFLGSLPQQLEVLNEQDTTHSIRPECSCSGHGKCCESSNTESSDDAGIQELDESSDEDDYNFCDTRQSFSDSAATPDLRMQCSNSSDGIHKYDHKFADSRYGKGDNEYLALPTKRRTKLPEPVEKEKGISLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCFEDLEYSQLLDRAYDYGLKGNSVMRTLCVAAFAVSGYASSDGRPCKPFNPLLGETYEADFPENGIRFFSEKVSHHPMVMACHCQGRGWKFWGESNVKSKFWGQTIQLDPVGVLTLEFDDGEIFKWSKVTTTINNLIIGRLYCHHHGTMKISGNRQHSCKLTFKQQSFLERNPRQVQGVVEDIGGTKVATLRGKWDESLYCSVTNDGVNSSAESTLLWEKHEPPTNLTRYNLSSFAITLNELTPNLKERLPPTDSRLRPDQRHLENGEYDKANSEKLRLETRQRMARKMQDNGWKPRWFDRDTEDGPFRYTGGYWEAREQRKWDGCRDIFGELPDKKTSPAPASVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCD0</accession>
    <name>A0A8I6YCD0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123451135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984078.1">
        <property type="nucleotide sequence ID" value="XM_045128143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCD0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0342170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0342170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0342170"/>
    </dbReference>
    <dbReference type="GeneID" id="123451135"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0285570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0285570.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0285570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0342170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0342170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0342170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451135"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46869">
        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46869:SF6">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0342170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="331" mass="36802" checksum="DD43A287625EC024" modified="2022-05-25" version="1">MPPRGRKHWCKHCKRSFPSGASLGGHMAVHRNRRKKQPSRTPSIDGERYGLRERCQKTSWLLDSVSSDDDRWALFPKTECQLCFRSFASPDALSMHMRTHKRRRKMAVEHQAAVGDGDDLNVPVPAAPVMRKKRSRRMVLDTVPSPVMEAYGMEEVDAACILVMLSGDHGMCPAFVDCGEGGEMDDVAFDALMTEMGLTSPDHHGPVGDNELMEPEPSSSYEEVKFVSLSKVLKATANYECKICGQVFTSGRALGGHRKRHSFADHGRAPTVTKSEATQPCEELVPPDHRSLVLNLPSPSIWNCNTARPKPEPNQLLVASSLWDERMLGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YE35</accession>
    <name>A0A8I6YE35_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YE35"/>
    <dbReference type="SMR" id="A0A8I6YE35"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0484160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0484160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0484160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0484160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0484160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0484160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46238:SF11">
        <property type="entry name" value="AGAMOUS-LIKE MADS-BOX PROTEIN AGL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46238">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="115"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0484160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="557" mass="65470" checksum="608BB86B3467FF2C" modified="2022-05-25" version="1">MAKIRERKTRDVGEVKCIKDGDDQLLVKDEAIKRRWREYFDNLYNGEVDISTIELDDSFDDTSRCFVRRIQECEVKEALKRMKVGKAMGPHGIPIEVWRGLGDMAIVWLTKLFNLIFRSNKMPEEWRRSILVPIFKNKGGVQSCTNYRGIKLMSHTMQLWERVIEHRLIRLTSVTKNQFGFMPGRSTMEAIFLVRQLMERYREQKKDLHMVFIDLEKAYDKIPQNVMWWALEKHKVPIKYITLIKDMYDNVVTSVRTSDGNTDDFPITIGLHQGSALSPYLFYLVMDEVTMDIQGDIPWCMLFADDVVLVDDSRTRVNRKLELWRRTLESKGFRLSRNKTEYMRCSFSATRHKEGEVSLGGQVVLERDTFRYLGSMLQKDGDIDEDVGHQIKAGWMKWRQASGVLCDKRVPQKLKGRFYRTAIRPTMLYGAECWPTKRRHIQQLGVAEMRMLRWICGHTRKDRVLNDDIRERLGVAPIEEKLVQHRLTWFGHIQRRPPEAPVHSGRIKRAENVKRGCGRPNLTWEESVKRDLKVWNIDKDLAMDRGAWKLAIHVPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YE91</accession>
    <name>A0A8I6YE91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MIZU-KUSSEI 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123410689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044959563.1">
        <property type="nucleotide sequence ID" value="XM_045103628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YE91"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676240"/>
    </dbReference>
    <dbReference type="GeneID" id="123410689"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0561170.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0676240.1">
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123410689"/>
    <dbReference type="OrthoDB" id="1859415at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010274">
        <property type="term" value="P:hydrotropism"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006460">
        <property type="entry name" value="MIZ1-like_pln"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31696:SF66">
        <property type="entry name" value="OS06G0495800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31696">
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    </dbReference>
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        <property type="entry name" value="DUF617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="30796" checksum="8AAA8663EDA61F20" modified="2022-05-25" version="1">MAGTLRAAVAASQPPPPPSPLPPSRGATASGGIPWLLRKRASKARHDPPLGGQEPDAHDEVEVELDDEGATFVVSTPCAADAAGAGGGTPERSGKRREALARLRSAVLAVVARARRRRGRRPMGSSVTGTIFGRRRGRVHLALQTDPRAPPALMVELAAYSTGALVREMSSGLVRIALECEKTAAHTGGERRRTALVEEPTWRAYCNGRKCGYAVRRECGAEEWRLLRAVEPVSVGAGVVPDGGAGEGDLMYMRARFERVVGSRDSEAFYMINPDGNGGPELSIYLLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YEA7</accession>
    <name>A0A8I6YEA7_HORVV</name>
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        <recommendedName>
            <fullName evidence="10">PXMP2/4 family protein 4</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946081.1">
        <property type="nucleotide sequence ID" value="XM_045090146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YEA7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0448490.1">
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    </dbReference>
    <dbReference type="GeneID" id="123395202"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0448490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0448490"/>
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    <dbReference type="OrthoDB" id="430207at2759"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF18">
        <property type="entry name" value="OS12G0508100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0448490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="28340" checksum="DAF75C3C0E65793F" modified="2022-05-25" version="1">MVTAGALHAGSRVLLPIRRTFSTPWSHVRSHLHSTKPSSAPLPPPPRPPPSSHAASTRFTAAPTRRSGSIGSGVVTWYLGSIEARPLLTKSITAAAIYTVADLTSQMITLPPEESLDLIRTLRMASYGMLFSGPSLHYWFNFISKVVPKRDVVSTFKKMFLGQAVYGPIINCIFFSYNAGLQGETIPEIIARLKRDIIPTIKNGLIYWPLCDFITFKFIPVHLQPLVSNSFAFLWTIYLTYMAGLKKPGMEVIMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF13</accession>
    <name>A0A8I6YF13_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401711</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951501.1">
        <property type="nucleotide sequence ID" value="XM_045095566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF13"/>
    <dbReference type="SMR" id="A0A8I6YF13"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611880"/>
    </dbReference>
    <dbReference type="GeneID" id="123401711"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0507380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0507380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0611880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611880"/>
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    <dbReference type="OMA" id="THHHADE"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000159">
        <property type="entry name" value="Homeobox-leucine zipper protein HAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF497">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="69"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="37241" checksum="0A598D3CAA8852BF" modified="2022-05-25" version="1">MEPGRLIFNTSGSGNGQMLFMDCGAGAAGMFHRGVRPVLGGMEEGRGVKRPFFTSPDDLLEEEYYDEQLPEKKRRLTPEQVHLLERSFEEENKLEPERKTELARKLGLQPRQVAVWFQNRRARWKTKTLERDFDRLKASFDALRADHDALLQDNHRLRSQVVTLTEKMQEKEAPEGCFGAAVDASESEQATAEAKASLADAEEQAAAAEAFEVQQQQQQQLHVKDEERLSPGSGGSAILDARDALLGGGCGLAGVVDSSVDSYCFPGGAGADEYHECVVGPVAGGIQSEEDDGAGSDEGCSYYPDDAAAVFFAAAQGQHHHHTDDDDQDDGQISYWMWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF67</accession>
    <name>A0A8I6YF67_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123399689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950018.1">
        <property type="nucleotide sequence ID" value="XM_045094083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF67"/>
    <dbReference type="SMR" id="A0A8I6YF67"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537490"/>
    </dbReference>
    <dbReference type="GeneID" id="123399689"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0447230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0447230.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0447230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0537490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399689"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000005">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF772">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="232"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="387" mass="42958" checksum="B8B15B1F7D16BF20" modified="2022-05-25" version="1">MYSYHLLKPGESMRVCKQPDLSILQIPSMASVDDHERLRAVKAFDDTKAGVKGLVDAGVTTVPSIFHHPPESLPSSEVVVPAHRFTIPLIDLLAGVRADMVSALKVAAETVGFFQLVNHGVPDDLLAEMLASVRRFHESPAETKRPYYSRDHRRRVRYNSNFDLFTSPAANWRDTVFLELPPAPEEIPVACKAVAPEYARQVQRLGRTLFELLSEALGLNPSYLEEETMCLDRLSMGGHYYPACPEPHLTLGTTRHSDLSFLTVLLQDDVGGLQVLVDDDGNKQRPAWVDVPPVEGALVVNVGDYLQLMSNDRFKSVEHRVVANTVGPRVSAACFIRPCASSKRVLTPIVIDGDGGARYRSTMVEEMFTHYRVKGLDGTSALQHFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLA9</accession>
    <name>A0A8I6YLA9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.</text>
            <dbReference type="EC" id="3.2.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome-inactivating protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980039.1">
        <property type="nucleotide sequence ID" value="XM_045124104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLA9"/>
    <dbReference type="SMR" id="A0A8I6YLA9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415130"/>
    </dbReference>
    <dbReference type="GeneID" id="123447495"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0415130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447495"/>
    <dbReference type="OrthoDB" id="696303at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030598">
        <property type="term" value="F:rRNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.420.10">
        <property type="entry name" value="Ricin (A subunit), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036041">
        <property type="entry name" value="Ribosome-inact_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001574">
        <property type="entry name" value="Ribosome_inactivat_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016138">
        <property type="entry name" value="Ribosome_inactivat_prot_sub1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453:SF44">
        <property type="entry name" value="RRNA N-GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00161">
        <property type="entry name" value="RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56371">
        <property type="entry name" value="Ribosome inactivating proteins (RIP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="1" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0800">Toxin</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="308"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="560" mass="60604" checksum="2B88AFC0C6316557" modified="2022-05-25" version="1">MALDKVAPIVIVTPFNVMTDSYDEFIENVRKALAGKVPDSPTVVGPKSEVARPVMDKGTTPVEQPPRWIHVELRGKTQGTTTPNPKVAIRSDDAYIMGFTNSTGRWFQLSKTGTKYKLVDDKAVMAGFEGNYDTLVGGVKHLPDLNLNKFSMAQAAAALWNKASTISGGIGNDDVDDDGDMLRANDPVKQAVATLAVAVCEAARFIPVSNVVKEGWSKDRVSVTPDEVNYIREWGDLSTALLSWKKKGYKDDATIFKIFNGIGITNGEEALAVVRLVKRVIRSNMADAPTTDEQLLAYAQLPKHGRYMAEVFAVRIPATAGGDPPSGTISLHGGHCSSDFIYSPEEEHTSQQTSCDSQGNMVLTGPSVATSAYGPIVFNLDLHDGTRGQADEEEDEENTGRIVCDAVGGDFSNYDKAISETVLTRCGPAEVIYAVLSNGVQGRVDVKLAGLQSRDEVVLVGRIVARSKLFDIGCVLFYNEAAGVRVRPGELVPLARHALAVPLHMPLTIELDIRHGGSGDEIVRGELEFKTAIDGLHTGRLVGVNDAEFEVTILWSEYPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YN18</accession>
    <name>A0A8I6YN18_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YN18"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0437120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437120.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525740"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525740.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="9360" checksum="A82C7173712DBDA0" modified="2022-05-25" version="1">MIQDASSWRQFGYLTFIAAGCWFDNNLYRYGMGYKPTFLYWSRTMRCLGDPFMAWNLLWLVLDKIDSPMARPFTWGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPG0</accession>
    <name>A0A8I6YPG0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPG0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0713640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48475">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48475:SF1">
        <property type="entry name" value="RNASE H TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25972" checksum="CF6687AF920ED0FE" modified="2022-05-25" version="1">MQKTVNRSTNNLSKNLDDALWAYRTAYKNPMGMSSYKMVNGKACHLPLELEHKAYWAIKELNFNFKLAGEKRLFDISSLDEWRIQAYENAMLFKEKVKRWHDKRIQKREFNVGDYVLLYNSRLRLFAGKLLSKWEGPYVVEEVYCSGAIKINNTKGNYPRVVNGQRIKHYISRTPINVESNIIKTITPEEYLRYIYQPVSDSENKEVCDLVRKESPKLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQ47</accession>
    <name>A0A8I6YQ47_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123452495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044985087.1">
        <property type="nucleotide sequence ID" value="XM_045129152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQ47"/>
    <dbReference type="SMR" id="A0A8I6YQ47"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456890"/>
    </dbReference>
    <dbReference type="GeneID" id="123452495"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0456890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452495"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000005">
        <property type="entry name" value="nitrilase homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="3">
        <location>
            <begin position="37"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="314" mass="34501" checksum="BC942C1AD5306991" modified="2022-05-25" version="1">MALAPWPPSRSLSHSPPPPPSSARAMSLSSVSAASQGKVGVVQMTSVGDLDANYATCSRLTKEAALAGVKFLCFPEVFSFIGSKDGESVKLAEPLDGPIMQRYCSLANESSMWLSLGGFQERGPDDSHQYNTHVLIDESGKVRSSYRKIHLFDVDVPGNMVYKESRFTTAGDTVLVVDSPFGRLGLTVCYDLRFPELYQCLRFKHQAQVLLVPSAFTKVTGEAHWEILLRARAIETQCYVIAAAQAGKHNEKRESYGDSIIIDPWGTVIARLPDRLSTGFAVADIDLSKVEAVRTKMPISEHRKFESIWKSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQX7</accession>
    <name>A0A8I6YQX7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maturase MatK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intron maturase 2 family. MatK subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQX7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0800090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0800090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0800090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0800090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0800090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0800090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34811">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01824">
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="335"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0800090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="392" mass="47140" checksum="6267ADADBF943A03" modified="2022-05-25" version="1">MEKFEGYSEKQKSRQQYFVYPLLFQEYIYAFAHDYGLNGSEPVEIVSWNNKKFSSLLVKRLIIRMYQQNFLDNSVNHPNQDRLLDYKIFFYSEFYSQILSEGFAIVVEIPFSLRELSCPKEKEIPKFQNLRSIHSIFPFLEDKFLHLDYLSHIEIPYPIHLEILVQLLQYRIQDVPSLHLLRFFLNYYSNWNSFITSMKSILFFQKENKRLVKFLYNSYVSEYEFFLLFLRKQSSCLPLAYSGTFLERIHFSRKMEHFGIMYPGFSRKTLWFFMDPLIHYVRYQGKAILASKGSFFFEKEMEMLPYQFLAILFLFLDSAAKNPYKPISKLLLRFYGIPFKCTKKSFVGKESNAGEFISHRYSNEKIRYHSPRYSPHRILIKSSILYWIGASY</sequence>
</entry>
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    <accession>A0A8I6YSW3</accession>
    <name>A0A8I6YSW3_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">DUF4283 domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YSW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747660.1">
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747660.1">
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33170">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33170:SF22">
        <property type="entry name" value="OS10G0417100 PROTEIN"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12652" checksum="CE2BC7ECBE01A6AB" modified="2022-05-25" version="1">MNVKSCGAVINLRKWSESVGAKGVLNVAWVNVSNIPLDKRHEKNIAYVGSLVGVTLEIDKSTINRPESVRIKLGCRDAEDIPASAEGVLGGHFYDFFYSVDKILIKNPPRERIGV</sequence>
</entry>
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    <accession>A0A8I6Z554</accession>
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        <recommendedName>
            <fullName evidence="4">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123403544</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953408.1">
        <property type="nucleotide sequence ID" value="XM_045097473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z554"/>
    <dbReference type="SMR" id="A0A8I6Z554"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631420"/>
    </dbReference>
    <dbReference type="GeneID" id="123403544"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0631420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31775">
        <property type="entry name" value="OS02G0117200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31775:SF43">
        <property type="entry name" value="OS02G0824500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="175"/>
        </location>
    </feature>
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        <location>
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            <end position="61"/>
        </location>
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            <end position="182"/>
        </location>
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        <location>
            <begin position="105"/>
            <end position="158"/>
        </location>
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        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="182" mass="19890" checksum="F298B946A9C0F417" modified="2022-05-25" version="1">METQREPTNAGEPAGGGGEVLEHARPAPAPAPATAAPPAPATATATASPAAPPASATDRDAVLAKVEMERKLSMVKAWEENQKSKADNRAEHKMSSILSWENTKKAAVEAKLRTREEKLEKKKAEYAEKMRNRVAMIHKEAEEQRAAVEAMRQEEMIKCQEMAAKHRSKGTTPKKKFLTCFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z5I2</accession>
    <name>A0A8I6Z5I2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5I2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0603190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0603190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0603190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0727220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035208261" evidence="2">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="158" mass="16683" checksum="9A71A620C5F1F5AC" modified="2022-05-25" version="1">MAARSAYKTPLVPTYLLTFSIALASSLRPVPAEWQRRDKSAPTPPTPHGPLRRLLRIPRPTPRTSAPPSLLPPSLRPSARPRSLVLVVVGESARPDGDMHSISLRCAGTGRPRLAAAVPASIAVAVGRFALQLLLGTPSPALFLPCHRVGFVVGGVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z5R8</accession>
    <name>A0A8I6Z5R8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5R8"/>
    <dbReference type="SMR" id="A0A8I6Z5R8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0730160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47853">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47853:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="2">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="265" mass="30024" checksum="861AD22B33BEA98F" modified="2022-05-25" version="1">MPNLLPTPPLDHTEEEKRARVRRHAMAASTQTSPLRRWKRFFRAFDCVDVAIKPSDELRRARGDIVEQLCDAADDDQAERLCGILDDHMVESLETLRLIPVVPNMLVSTDLAKSVCALRRHESERVRGLARGIVSGWRSSMQDDLAKVRDALHKIDNINMLQTKEVAVNQQQHVSANSDIVMKTVGISKNSSDLVAGDRAGLCYEEKMAAAKRKFHQSYQEAEDAKRRRRTKLVQAPKMMQPVRRCTTSSTVKKTISIRSQLHMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z5W6</accession>
    <name>A0A8I6Z5W6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Synaptotagmin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123412720</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961595.1">
        <property type="nucleotide sequence ID" value="XM_045105660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5W6"/>
    <dbReference type="SMR" id="A0A8I6Z5W6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731790"/>
    </dbReference>
    <dbReference type="GeneID" id="123412720"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0607230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0607230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0607230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0731790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731790"/>
    </dbReference>
    <dbReference type="OMA" id="FPCFGTV"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000066">
        <property type="entry name" value="Extended synaptotagmin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000102">
        <property type="entry name" value="Synaptotagmin-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF217">
        <property type="entry name" value="OS06G0685300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="13">
        <location>
            <begin position="66"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="239"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="402"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="61677" checksum="F308E7AD09A40A30" modified="2022-05-25" version="1">MGLLGGLVGFVLGLPLGLAAACLVYLRFFAPRRRLQDPVVRPLRDLDNETLQTMVHDIPLWVKYPDYERIDWMNKFICDMWPFLDKAICKIIRTVAKPICDQYVGKYGIESIEFGNLTLGALPPTLQGIKVFEMREKELVIQPVIRWASIANVIVNVKVHSFKLSVELLDLHMMLTPRVTLKPLVPSFPCFASLCVSLMEKPDVDFGLKLLGGDVMAIPGLYRFVQDQISKQIAILYHWPKVIEVPILDGASGATKKPVGILQVKVIRAMNLPKMDLLGKSDPYVKLRLSGERLPSKKTSVKMSNLNPEWNEHFRLVVKDPETQVLELQMFDWEKVKMHDKMGVQVIPLRLLTPCESKLFTLDLLRSMNPNDQQNKKNRGKLVVELTFDPFREENNTSPLISDGEGNISLKRDVPDGGGVLLVSVENAEDVEGKRHTNPYAVVLFRGEKRETKVMRKARDPRWNEEFQFVVDEAPMDEKIHIEIRSRRRRLLPFRNQESLGHVNINLVDVVNNGRINEKYHLINSRNGKLQLEIKWNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z6D0</accession>
    <name>A0A8I6Z6D0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6Z6D0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643240.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0643240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643240"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
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    </dbReference>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="178" mass="20220" checksum="86A0DB224B3C7312" modified="2022-05-25" version="1">MSWSNCESSAPGFRGASGRRRGTEARSPVPYRQNPMAYEPEVLCHCNPRRKAPRWISWSRANPGRRYYSCVNAMNGDGCGFVQWYDSPLPKFFSDLIGDLRDEVWRLHGQDNVPPVSVEQVEECRDQFVLVEPTVKDLQDQLKEKSAEIAALKGKFEKVVFIVLVFVFGVVAGKLFAF</sequence>
</entry>
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        <lineage>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="13"/>
            <end position="70"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="94" mass="10591" checksum="63FC4EAE12FC79FD" modified="2022-05-25" version="1">MSALEILQFFMAIDCYPNVSVAYRILLTVPVTVVSAEGSFSKLKLLKNYLRSTMLQDRLNGLAMYCIEKYILDNLDLDCALNDFASRNSSLRAS</sequence>
</entry>
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    <accession>A0A8I6ZE71</accession>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="4" key="1">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
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        <location>
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    </feature>
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        <source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="156" mass="17983" checksum="EDE002E04E152859" modified="2022-05-25" version="1">MPVLTIVDRPVPISSRAPPCAVVLVSASVGPRPVYDSDVEVCEPEIPTHIEILVENLNLVEKENKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAVGSILTLVAILIAFFVLFKCFT</sequence>
</entry>
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    <accession>A0A8I6ZEF9</accession>
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        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZEF9"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0609580.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0734880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734880.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33286">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33286:SF16">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR_PLANT LIPID TRANSFER PROTEIN_SEED STORAGE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035259216" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="SignalP"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13621" checksum="8481C83261FEE340" modified="2022-05-25" version="1">MTSVKAIGVFCFLVFVSLSSYPHHAQADHCAVDKSKVMRDCWRNIGKNVGEHPLLHGSVCCQVIRAATDIHCVCDKFTAHELARISLAKFAMATHVCGNGLRAHTHCAGYTVPVITLPAPPPPEST</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199790.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11964" checksum="2163E50392B203D7" modified="2022-05-25" version="1">MVSFYWPPMEIMGRGETTELVDSSVVVSCPLHEALRCIHVGLQCVQDHPSDRPLMSSVLSMIENESGLLPTPKQPVYFTTSNCEGEEARENMENSFNTISITTLESR</sequence>
</entry>
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        <lineage>
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    <reference evidence="9" key="1">
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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            <end position="96"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="22024" checksum="7CA7E16C2649C52E" modified="2022-05-25" version="1">MEFFTVILEKSSRLVLPHNFVKMLDGHRPQNMKLRQAGNGLRKLWDMEVVFDADGRMYLDRGWKQFVRAYDLEIGYFLVFRYDGNATFDVKVFDTTMCQRRYQDDDDAGNGSRSSEYCDRCYVYGSSDSGSKSSSDSGDSKSSSDDGLAMDVPQLGDMAMPIVVEKYIPQPGLGLRRSKRIKMMKEKVKKQE</sequence>
</entry>
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                <person name="Fincher G.B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A8I7B6B7"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0261430.1.CDS.1"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0312920"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF192">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="240"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0312920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="301" mass="32640" checksum="8885C87B6D5D1A5E" modified="2022-05-25" version="1">MLPTSTSSRLHPPKRPSQDPTNTIAPAMAMSSQRESASTVQVDSRMERFKMYPMAGATPPAPREGNGEPPRQRLFVRIRCSVRMSTETHLVGGTTLRQGHGKDADARHPEEEAAARRFIVHDPAVLRSEPACHAAVRRMLTHLPQLAGYYGHRAADAQLWDRFVPPGLVARIVARVAREDGLVAAGGAARRSQMCHVWLRVHVTSVYSVPKALLLSCEDAAAGARAGSGALVGGGGAAQCAICMEEMVAGGVGVVALPGCSHAFHRGCILKWFHRAATCPSCRRDMMHHVPEMCRIWHMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6P9</accession>
    <name>A0A8I7B6P9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6P9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37736">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37736:SF1">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="23763" checksum="6BA8DDD8E27E7ECC" modified="2022-05-25" version="1">MMWFESTYDSCVLFSSFGTTSELVKEFETKGTISETKEGPVLSVVSKRLRMLRKKQNHIVQMEESVAADKMLNQEQKEVMRSKPVIAALIDELERLRMPLSTALTKEPSTVPGPASSSSSFVSNLSIQDLLVLMYFSSLFYVKSPNEFIATMVAHKHERSSCITHGYVWGDTVDLLTENDLDAVSAVAALAAAPPASAVGVSHHDSRTVPIMPTYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7B0</accession>
    <name>A0A8I7B7B0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123446596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979108.1">
        <property type="nucleotide sequence ID" value="XM_045123173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7B0"/>
    <dbReference type="SMR" id="A0A8I7B7B0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0384470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0384470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0384470"/>
    </dbReference>
    <dbReference type="GeneID" id="123446596"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0319760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0319760.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0319760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0384470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0384470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0384470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446596"/>
    <dbReference type="OMA" id="RMEDWRM"/>
    <dbReference type="OrthoDB" id="686682at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044823">
        <property type="entry name" value="ASIL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF44">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0384470.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="27822" checksum="33B9072A88DC699D" modified="2022-05-25" version="1">MTGGKTGPGASRRWSDGETCVLVDAWGPLYIHRNRSSLRMEDWRMVCSAVNAHHAAAGLDLNRNEAACKRRISILTAQYYKEEVAKGGLTSGWRHFARLRSFLDDPIGPPPGFDAKTPPGPSVKKEKKVEEASGCVGESAGKTKVQAKRQPRHFSLHDIYERSGGPSLEKTPATIKDEEVVGCGCELVGGSPAGVTKLVAEVTKLAEVFERVERERFKFEKEMRKVKKEEMETEDVNLERKKKAKAENPDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7Z8</accession>
    <name>A0A8I7B7Z8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7Z8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0425040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0425040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0425040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0425040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0425040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0425040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0425040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="19201" checksum="DDA5EBBE97247348" modified="2022-05-25" version="1">MCLRDQHLWRVRCPMICFYAVEWHLPHRVSKQFGVQQRTPPDYVETSVNLHKINRQSNKTVINWEEHHMTWVDMWNAQRQPRVENDQTPDTDEATYLRHLEWMRTEYRVIHKAAWTRSDCLDLLPSEAADAAFNNSIRETIGAHLDYGPLHDQVVHPSFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B878</accession>
    <name>A0A8I7B878_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B878"/>
    <dbReference type="SMR" id="A0A8I7B878"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF133">
        <property type="entry name" value="OS07G0196600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="424" mass="47677" checksum="31323A87E355857C" modified="2022-05-25" version="1">MMEGKRPTHHGGYEMSEDLLMEIFSRLPAHSAARCAALSRRWRKIVDGPDFWIRHHRPPSPNFNMAYIHEYTGEGEGEHRPLQHAFHLPGLLAQGLLSNDRHGLAYRYVGTCNGLVALQVEGLYHHSFFACVVLNPATTEEVTLTYLGLCEDHYLCGFGYGPASKTYKLLVVRRPYGCFFGGPLEVLSLGDARGEARAVFPNVWCNDTFSLCIGGVLYVLDYKHLSVLALDVDSETISAVELPVGGVVCSEPMEVRGRLCIAMHTDETHEFVLWVLTASLDWERRCVLRYMVPTTLRSHWLVGAWDSGENRLLVWYRGAGLRLYDLREMDSGGAHEPAGSWTLEDQKDLRDYMRWGYGQYRLCWGYRPTTISPRVTIANCAGVPPVLCSFRSGRQTPQRPVAPFSNVLLDLVKTILVGQEVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8T7</accession>
    <name>A0A8I7B8T7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8T7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035272051" description="3'-5' exonuclease domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24813" checksum="234F9F02F6076BD5" modified="2022-05-25" version="1">MKSTNRSRVAISLFLLLSRSRSLFFSSLLPFSSSTMPTRFGEVLAHGKTMLDVVYTNESREMPYFLEQLKERWLDAAMDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRSGITFATVDITNDKLKMSTSMAHMAVALIDEEYGDMKTNFPKSHHKLWEKAPLDRINIEYAAKDAYVSYELYRKIRVVNYGQRHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9G5</accession>
    <name>A0A8I7B9G5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950114.1">
        <property type="nucleotide sequence ID" value="XM_045094179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9G5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0494490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0494490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0494490"/>
    </dbReference>
    <dbReference type="GeneID" id="123399797"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0409870.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0409870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0494490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0494490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0494490"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="660486at2759"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF431">
        <property type="entry name" value="OS02G0305950 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0494490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14185" checksum="32FDFFD11BBBF757" modified="2022-05-25" version="1">MGMSEKSLAKRKAGLITKTLDRCWSTPMRNKPAEGCFSVYVGAGRQRFVVRTECVNHPLFRALLEEAEEVFGYASAGPLELPCNAEAFAGVLEQIKEEKQMAGGRRCGLSRGKSYRLLGTGQTVIIDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAE7</accession>
    <name>A0A8I7BAE7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAE7"/>
    <dbReference type="SMR" id="A0A8I7BAE7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010179">
        <property type="term" value="F:IAA-Ala conjugate hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009850">
        <property type="term" value="P:auxin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08017">
        <property type="entry name" value="M20_IAA_Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044757">
        <property type="entry name" value="ILR1-like_Hyd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF159">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="191"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="417" mass="43544" checksum="A5D70456CD1A8773" modified="2022-05-25" version="1">MAAAAALGAELLEEARAPGFAAWVRGLRRQIHQHPELAFQEHRTSALVRAELDALGVPYAWPVARTGVVATIAGGVPGPVFALRADMDALPIQEMVEWEFKSKEDGKMHACGHDAHTAMLLGAAKLLQSRKDSLAGTVKLVFQPAEESHAGGYHVLQSGVLDDVAAIFAVHVDTNLPAGAVGSRPGPFLAGSARFKAIITGKGGHGAMPHAAVDPVVAACSAVLSLQQLVARETNPLQGAVVSVTTIRGGEAFNVIPESVTLGGTLRSMTTQGMGYLMTRIREVVEGQAAVGRCAATVDFMEGELRPYPATVNDEGVYAHARAVAEGMLGPANVRLSPQIMAAEDFGFYAEKIPAAFFGLGVRAGGEEDEVHHVHTPRLVIDEEALPVGAALHAGVAIEFLNKHARACATASPPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB15</accession>
    <name>A0A8I7BB15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB15"/>
    <dbReference type="SMR" id="A0A8I7BB15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0317790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317790"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF306">
        <property type="entry name" value="SERPIN-Z1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="26187" checksum="5345A7A3E63E71D6" modified="2022-05-25" version="1">MHVFADSSDDDAEPKVQFANAVWANATAEPLNPDYARVITQHYRAQAREASFTTMNTIPKSSCRHRLPPRLQGTETPLRVWHRRRFAMYVYVPNERHGLQGILHRLASSPEQLETDSMALRTSVAVGAFKVPKFTVSHKTKASEMLQSLGLRLTFSPAADFSELLAWPNLPLIVSQVYLESFVEVNEEAIACILGSCAGGWTPVDFVADHPFMFLTKEELTGVIVFASQVVNPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCY5</accession>
    <name>A0A8I7BCY5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BURP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0637060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044816">
        <property type="entry name" value="BURP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004873">
        <property type="entry name" value="BURP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236">
        <property type="entry name" value="BURP DOMAIN PROTEIN USPL1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236:SF7">
        <property type="entry name" value="BURP DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03181">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01045">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51277">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035266342" description="BURP domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="BURP" evidence="3">
        <location>
            <begin position="335"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="559" mass="62502" checksum="7519CFCFFD31AD0B" modified="2022-05-25" version="1">MTAMMSILSLFLLVTTVWGGPRANLTNVEGIVVSNSAAELTEYWQTTVPNSPMPLAILELLDTSTGHDDHMGKQDTDNNRTPNKWTNEKYVGASLLNSRRYGLHPNIDGKARTKSGNQKNQMWFYGWSPQKANNKKTTEGLAREPVKQMNDKKNQMWFYGWGPEKVNNEKTTKGLAREPVKQMNHKENQMWFYGWSPQKANNEKTTEVLAREPVKQMNDKKNQMWFYEWGPEKVNNEKTTKGLAREPVKQMNGKENQMWFYGWSPQKVDNEKTTEVLAREPVKQMNNKKNQMWFYGWGPVQISNDDGARESAVREDDGHQGESHIHGESSVSNIIFFEESLKPGSTIAPYIQPSATSGAPLLQRDVANTIPMSTGNLTDILAMFAPVSHAMAKDVWMMLDLCERPSLVKGEKKTCANSVESMVEFAAYVLTGGNTRGLRAFSSPDVPAEGVTSGRKYKVVAARRATESSETMTCHGMSFPFVLFMCHAVNPTRTYEVTLESEDLTSTMVSLAVCHLDTSEFDPKKMPGHAKPGDAPACHFIPRDSVLWAPVAPAASAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BD63</accession>
    <name>A0A8I7BD63_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123447165</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979681.1">
        <property type="nucleotide sequence ID" value="XM_045123746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BD63"/>
    <dbReference type="SMR" id="A0A8I7BD63"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406540"/>
    </dbReference>
    <dbReference type="GeneID" id="123447165"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0338430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0338430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0338430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0406540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447165"/>
    <dbReference type="OrthoDB" id="684567at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF73">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="50"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="313" mass="33851" checksum="CB1622A65F8C7582" modified="2022-05-25" version="1">MALEAVVLAQGHFGYGLQAPGLAPWCDMLGAGGFLEDDWDQQLLAAPVAPNAEDQWEPVSSWDQSEASSEGKAAAPPEPATAAAATGRRKRRRAKIVKNKEEVESQRRTHIAVERNRRRQMNEYLAALRLIMPQSYAQRGDQASIVGGAINYVKELEQLLQSLEVQKSVKNRTGRSPFASSFTFPQYSASCNITQATTASDAGSGADSSGIKSEAGVADIEVTMVEGHASLKVLARRRPKQLLRLVAGLQQLRIPALHLNVTTVDAMVLYSFSLKVEDGSKLGSVEDIAAAVHEILGRIQREEEEAGRLSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEQ5</accession>
    <name>A0A8I7BEQ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GIR1-like zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEQ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0707770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055281">
        <property type="entry name" value="GIR1-2/SIED1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056440">
        <property type="entry name" value="Zn-ribbon_GIR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33177:SF20">
        <property type="entry name" value="OS08G0102250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33177">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24747">
        <property type="entry name" value="Zn-ribbon_GIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIR1-like zinc ribbon" evidence="2">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="139" mass="15086" checksum="2944B9C45DF78D03" modified="2022-05-25" version="1">MHSSFPKPLYNVLPVGGAPHDHSSQFLTHHRTDTEGLLSSSSSSSNHLHCTVASLTPLSEPSMSQITTSSGEVSMDSPASSCVSSDAEVEMPMVVAGCPQCLMYMMLSDSEEEKQPKCPRCKSPVLLHFHKYCDTKNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIC5</accession>
    <name>A0A8I7BIC5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase/fosfomycin resistance/dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIC5"/>
    <dbReference type="SMR" id="A0A8I7BIC5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654180"/>
    </dbReference>
    <dbReference type="OMA" id="FKHVMLM"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052164">
        <property type="entry name" value="Anthracycline_SecMetBiosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33993:SF14">
        <property type="entry name" value="GB|AAF24581.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33993">
        <property type="entry name" value="GLYOXALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase/fosfomycin resistance/dioxygenase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="12944" checksum="AFB6B74C33A9A35A" modified="2022-05-25" version="1">MAAATLRWVVQMHRDVPRAARFYAEGLDFSVNVCTLRFAELQSGPLKLALMHTNDSTLATQRAYSSMMSFTVPDITSTVTKLMSLGAELDGPIKYEVHGKVAALRCIDGHVLGLYEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQK2</accession>
    <name>F2CQK2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADPH-protochlorophyllide oxidoreductase</fullName>
            <ecNumber evidence="9">1.3.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123432830</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot</tissue>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>chlorophyllide a + NADP(+) = protochlorophyllide a + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83348"/>
            <dbReference type="ChEBI" id="CHEBI:83350"/>
            <dbReference type="EC" id="1.3.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.33" evidence="9"/>
    <dbReference type="EMBL" id="AK353904">
        <property type="protein sequence ID" value="BAJ85123.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359203">
        <property type="protein sequence ID" value="BAJ90414.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366762">
        <property type="protein sequence ID" value="BAJ97965.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971353.1">
        <property type="nucleotide sequence ID" value="XM_045115418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQK2"/>
    <dbReference type="SMR" id="F2CQK2"/>
    <dbReference type="FunCoup" id="F2CQK2">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2CQK2"/>
    <dbReference type="PaxDb" id="4513-MLOC_73304.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0047060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0047060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0047060"/>
    </dbReference>
    <dbReference type="GeneID" id="123432830"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0036700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0036700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0036700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0047060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0047060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0047060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123432830"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="UniPathway" id="UPA00668"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CQK2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016630">
        <property type="term" value="F:protochlorophyllide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09810">
        <property type="entry name" value="LPOR_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005979">
        <property type="entry name" value="Prochl_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01289">
        <property type="entry name" value="LPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44419">
        <property type="entry name" value="PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44419:SF20">
        <property type="entry name" value="PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 9" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ85123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ97965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0047060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="42148" checksum="BFCB90122D6F64B9" modified="2011-05-31" version="1">MALQAATSFLPSALSARKEGAAKDSAFFGVRLADGLKLDATSLGLRTKRVNTSSVAIRAQAAAVSAPTATPASPAGKKTVRTGNAIITGASSGLGLATAKALAESGKWHVIMACRDYLKTARAARAAGMPKGSYTIVHLDLASLDSVRQFVKNVRQLDMPIDVVVCNAAVYQPTAKEPSFTADGFEMSVGVNHLGHFLLARELLEDLKASDYPSKRLIIVGSITGNTNTLAGNVPPKANLGDLRGLAAGLNGVGSAAMIDGAEFDGAKAYKDSKVCNMLTMQEFHRRYHEETGVTFASLYPGCIATTGLFREHIPLFRLLFPPFQKYITKGYVSEEEAGKRLAQVVSEPSLTKSGVYWSWNKNSASFENQLSEEASDTEKARKVWELSEKLVGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSA8</accession>
    <name>F2CSA8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AK354510">
        <property type="protein sequence ID" value="BAJ85729.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CSA8"/>
    <dbReference type="SMR" id="F2CSA8"/>
    <dbReference type="FunCoup" id="F2CSA8">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2CSA8"/>
    <dbReference type="PaxDb" id="4513-MLOC_66503.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0094050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0094050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0094050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0094050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0094050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0094050"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_4_1_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CSA8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000083">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF38">
        <property type="entry name" value="OS10G0497600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="189"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="459"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ85729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0094050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="508" mass="56602" checksum="A705E7FE63294CEF" modified="2011-05-31" version="1">MPTAESLRAELSSKTPPFGLRLWIVIGISIWVVIFCILGFMCFWSIYRRKPKKSVDKIPVSQIPDVSKEIAVDEVRQHAVVENYQVQESHTLTVQEKPHDKDSGKMLGHLVRTKSSDADNLSQCSSAYQCDRAGSAYSGDEGSSGNARRQYPQYATVSASPLVGLPEFSHLGWGHWFTLRDLEHSTNRFSKENIIGEGGYGVVYRGRLINGTDVAIKKLLNNMGQAEKEFRVEVEAIGHVRHKNLVRLLGYCVEGIHRMLVYEYVNNGNLEQWIHGAMRQHGVLTWEARMKIVLGIAKALAYLHEAIEPKVVHRDIKSSNILIDEDFNGKLSDFGLAKMLGAGKSHITTRVMGTFGYVAPEYANTGLLNEKSDVYSFGVLLLEAVTGRDPVDYGRPANEVHLVEWLKMMVGTKRADEVVDRDMEVKPTIRALKRALLVALRCVDPDSEKRPTMGHVVRMLEAEDVPSREDRRSRRGGHAGGNADAESKASSSEFEVSGDSGPSARFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5M9</accession>
    <name>F2D5M9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123394916</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK359189">
        <property type="protein sequence ID" value="BAJ90400.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK365815">
        <property type="protein sequence ID" value="BAJ97018.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945735.1">
        <property type="nucleotide sequence ID" value="XM_045089800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5M9"/>
    <dbReference type="SMR" id="F2D5M9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529770"/>
    </dbReference>
    <dbReference type="GeneID" id="123394916"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0440720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0440720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0440720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0529770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394916"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000001">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="10"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="182"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ90400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ97018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="375" mass="40676" checksum="DDEF9073630696C7" modified="2011-05-31" version="1">MGREKSRRLSGSRDFRQRLVLATLTSTPIVIKDIRAGEGGLRPHEMSLLHLLDKVSDQHAIDVNDTGTKVGYKPGVIVGGKDLEHDCGVHRGIGYFIEPLVLLGLFARSPLSIRLKGITNDAKDPSVDTFRMVTLHMLKHFGVPLEGLELKIECRGTPPCGGGEVLLRVPNINSTLKAVNWVDEGMVKRIRGVTFATKVSPQIGTRIVYAARGLFNKFIPDVHIFTDHRVGLSGGLSTGYGLSVVAETTTGCLISADSSVSYPNVDKMSEQSKKPELMSPEDLGEQVASMLLEEVAQGGVVDSTHQGLLFMLCALCPPDVSKVRVGQLTPHAIESLRNIKEFLDVKFIIKPDPNSNTVILKCVGAGVKNLARKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F2DE43</accession>
    <name>F2DE43_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123402374</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="AK362160">
        <property type="protein sequence ID" value="BAJ93364.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952227.1">
        <property type="nucleotide sequence ID" value="XM_045096292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DE43"/>
    <dbReference type="SMR" id="F2DE43"/>
    <dbReference type="STRING" id="112509.F2DE43"/>
    <dbReference type="PaxDb" id="4513-MLOC_66998.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541370"/>
    </dbReference>
    <dbReference type="GeneID" id="123402374"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0450190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0450190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0450190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0541370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402374"/>
    <dbReference type="eggNOG" id="ENOG502QRSY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034992_2_0_1"/>
    <dbReference type="OrthoDB" id="633494at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DE43">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010208">
        <property type="term" value="P:pollen wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF107">
        <property type="entry name" value="CHALCONE_STILBENE SYNTHASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="4">
        <location>
            <begin position="25"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="254"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="324"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ93364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="413" mass="44101" checksum="E3A400B22B834B41" modified="2011-05-31" version="1">MGSIGTTNGNGIGHGSAAMAAPAWRQHAHAEGPAAILGIGTANPTGVEIPQDIFAENLFRVTKSDHLTHLQHKLTRICEKTGIDKRHFHLTEETLVAHPELYDHEAPSLDNRIAMTVDAVPKLAQRAADKAIADWGRPASDITHLVFSTYSAWGAPSADLRLATLLGLRPTVSRTILSLHGCYGGGRALNLARELAENNRGARVLVACAEITLVCFGGPDGGNLVGHALFGDGAGAVIVGAGPFRDGEQSPIFEMVHATQTTLPKTEHALGMQVSGSGVDFHLAIQVPTLIGQNVERCLLHAFRGGDDDDGDGGAARLPCPLSGNGKWNDLFWAVHPGGRPILDNIDKVLELEPEKLAASRHVLREYGNMSGATIVFVLDELRRRRSMLPEWGAMLAFGPGVTIETMVLRCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJ70</accession>
    <name>F2DJ70_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123425474</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IAA-amido conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AK363938">
        <property type="protein sequence ID" value="BAJ95141.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965111.1">
        <property type="nucleotide sequence ID" value="XM_045109176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJ70"/>
    <dbReference type="SMR" id="F2DJ70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128980"/>
    </dbReference>
    <dbReference type="GeneID" id="123425474"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0106420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0106420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0106420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425474"/>
    <dbReference type="HOGENOM" id="CLU_016249_2_1_1"/>
    <dbReference type="OrthoDB" id="10004661at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004993">
        <property type="entry name" value="GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055378">
        <property type="entry name" value="GH3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055377">
        <property type="entry name" value="GH3_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901:SF96">
        <property type="entry name" value="INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03321">
        <property type="entry name" value="GH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23572">
        <property type="entry name" value="GH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23571">
        <property type="entry name" value="GH3_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH3 middle" evidence="3">
        <location>
            <begin position="373"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="GH3 C-terminal" evidence="4">
        <location>
            <begin position="466"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ95141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="617" mass="67835" checksum="C493BB277D158947" modified="2011-05-31" version="1">MAAMTDVSRAIGAALRSPASSPVAEAAAAAKAKDAEKLRFIDEMTCNVDSVQERVLAEILARNVDTEYLKNCGLDGAADRDTFRAKVPVVSYDALQPYIQRIVNGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIKDELDRRQLLYSLLMPVMNLYLSGLDKGKGLYFLFVKSETKTPSGLTARPVLTSYYKSEQFKNRPYDPYHNYTSPTAAILCADAFESMYAQMVCGLCQRHEVLRVGAVFASGLLRAIRFLQLNWEELAADIEAGALTPRVTDASVREAVAGILRPDPELAQFVRDECCKGDWAGIVRRIWPNTKYLDVIVTGAMAQYIGTLKYYSGDLPMACTMYASSECYFGLNLRPLCDPSEVSYTIMPNMGYFEFLPVDEATGAASCVDAGNLVDLARVEAGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFQFVRRKNVLLSIESDKTDEAELQRAVERAATLLRPHGASVAEYTSQACTKSIPGHYVVYWELLTTGAGAGATAVDKGTLDACCLEMEEALNTVYRQSRVADGSIGALEIRVVRGGTFEELMDYAISRGASINQYKAPRCVTFPPIIELLDSRVVSSHFSPALPHWTPARRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJQ6</accession>
    <name>F2DJQ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123445122</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
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        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045122058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307240"/>
    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0256690.1">
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307240.1"/>
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        <property type="component" value="Chromosome 3H"/>
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    <dbReference type="ExpressionAtlas" id="F2DJQ6">
        <property type="expression patterns" value="baseline and differential"/>
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Amino acid permease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="65"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="468"/>
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    </feature>
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        <location>
            <begin position="37"/>
            <end position="468"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307240.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMM5</accession>
    <name>F2DMM5_HORVV</name>
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        <recommendedName>
            <fullName evidence="6">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123447162</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406420.1.CDS1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0338320.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0338320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0406420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406420"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="PUP_plant"/>
    </dbReference>
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        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF97">
        <property type="entry name" value="PURINE PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ96346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="391" mass="41034" checksum="AADCF5A850E1964A" modified="2011-05-31" version="1">MATITASAISAMQEAAKTPSASPARAMAASMASPTRYRPSLLVIFSACLVLIGAGGPLLLRVYFVHGGQRLWLSALLQISGWPLLLPPMCVSLFRGRRHGIANLLLPPRLVVAAAVLGGFYAVSCYVYAMGSQALPLSTSSLLLATQLAFTAVFAFLFVGLRFTPFSANAVLLLIIGPAVLGVGPGAGKPAGVTSKAYWTGFCEGIAAAALAGLVLPLVEVSMERYGRRTGPAARAPPPYSTVMQMQAVMGAAGTLVCLLGMAIKSDFGALRSEAAAFGLGETKYYLVLVWDAVSWQLLNLGIMGLITFASSLLAGIMIAVLLPLSQILAVLFLHEKFDGPKGIALVLSLWGFASYMYGEKVQQKKAEAHKSELLQQQVASKTGDLELAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2DPD9</accession>
    <name>F2DPD9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123452607</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK365757">
        <property type="protein sequence ID" value="BAJ96960.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985221.1">
        <property type="nucleotide sequence ID" value="XM_045129286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DPD9"/>
    <dbReference type="SMR" id="F2DPD9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517240"/>
    </dbReference>
    <dbReference type="GeneID" id="123452607"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0429820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0429820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0429820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452607"/>
    <dbReference type="OrthoDB" id="690085at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007656">
        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31422">
        <property type="entry name" value="BNAANNG28530D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31422:SF5">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04576">
        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GTD-binding" evidence="6">
        <location>
            <begin position="52"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ96960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="40322" checksum="06C1417FE2B17F7E" modified="2011-05-31" version="1">MDQLALPSSSCSPQEQRRSVKRRPPPAGSPEPSPRAGAGPAELLRRVEELEAEARRLAMEKEAAEESALGLQGELEAERASAETAASEAMLMIERLQREKAAAQMEARQFRRYAEGRADHEREVQEELASLSDLAASYHSRLQSHGIDPDTFSDEEDEELYEEQRGHADQIDLVAPEAEGDGADLLIASANGMEVKAMVEEQEQEQSTTPVEKEFEYTVDVRCASPTMAAVAVVGEYVGDVAGNAGGLYARVEALEADRAAMHREIAALRAERAQLVMAREMARRLCWEVVSEKKSIAKQALVPAKRFSALGICKWLLSVIFWRRSSTTRYTFGLSTTFLGLLLLLDRSTMLSPWRRPHRPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQY1</accession>
    <name>F2DQY1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lipid IVA 3-deoxy-D-manno-octulosonic acid transferase</fullName>
            <ecNumber evidence="2">2.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123444853</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>lipid IVA (E. coli) + CMP-3-deoxy-beta-D-manno-octulosonate = alpha-Kdo-(2-&gt;6)-lipid IVA (E. coli) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28066"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58603"/>
            <dbReference type="ChEBI" id="CHEBI:60364"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.12" evidence="2"/>
    <dbReference type="EMBL" id="AK366299">
        <property type="protein sequence ID" value="BAJ97502.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK375315">
        <property type="protein sequence ID" value="BAK06510.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977648.1">
        <property type="nucleotide sequence ID" value="XM_045121713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DQY1"/>
    <dbReference type="SMR" id="F2DQY1"/>
    <dbReference type="STRING" id="112509.F2DQY1"/>
    <dbReference type="PaxDb" id="4513-MLOC_6249.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300960"/>
    </dbReference>
    <dbReference type="GeneID" id="123444853"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0251080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0251080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0251080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0300960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444853"/>
    <dbReference type="eggNOG" id="ENOG502QSA5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DQY1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043842">
        <property type="term" value="F:Kdo transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11720:FF:000001">
        <property type="entry name" value="3-deoxy-D-manno-octulosonic acid transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000032">
        <property type="entry name" value="3-deoxy-D-manno-octulosonic acid transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11720">
        <property type="entry name" value="3-Deoxy-D-manno-octulosonic-acid transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007507">
        <property type="entry name" value="Glycos_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038107">
        <property type="entry name" value="Glycos_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039901">
        <property type="entry name" value="Kdotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755:SF1">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC ACID TRANSFERASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04413">
        <property type="entry name" value="Glycos_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="3-deoxy-D-manno-octulosonic-acid transferase N-terminal" evidence="8">
        <location>
            <begin position="46"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ97502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK06510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="454" mass="50370" checksum="5B41091B75D0F875" modified="2011-05-31" version="1">MRPAHAPASAARGGRALYELYRAASRAAAPAVFLWRRMRGLEHRSRWPERLGRPSVARPRPGSPLVWFHAVSLGEGMAALPVVRHCARLHPGLPILLTTTTLSAFEVIKDLLPDVVIYQFAPLDCPDAIERFIGYWKPSLILLMESELWPNLIMSAAEKGIAVALLNARMSLKSFNRWSVPLGLPLISLMLSKLLLVIPLSTVQAVRFQLLHTPPHKIHFAGDLKYAVGDVEVGEKELDTIKDLQRQFNDRPIWMAASIHSGEEEVVLRVHDELIKVYPLLLLILVPRHPQDIKNVSLALKKQKINFVLRSTMEVVSINTRVYVVDTLGELRMLYRVTPIAVVGGSFLPSLSGHNFSEAAAAGCAVLTGPHVGHFYHMLVEMWQINPLAVKQLTGEAELLDVLKELLDDSKALEACQGAAKDAFSIMAHGVVSRVWNLVSTFAIDSQQKRGIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DRT5</accession>
    <name>F2DRT5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123403053</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK366603">
        <property type="protein sequence ID" value="BAJ97806.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372070">
        <property type="protein sequence ID" value="BAK03268.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952962.1">
        <property type="nucleotide sequence ID" value="XM_045097027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DRT5"/>
    <dbReference type="STRING" id="112509.F2DRT5"/>
    <dbReference type="PaxDb" id="4513-MLOC_53130.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631910"/>
    </dbReference>
    <dbReference type="GeneID" id="123403053"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0524870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0524870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0524870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0631910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403053"/>
    <dbReference type="eggNOG" id="KOG3345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068345_0_0_1"/>
    <dbReference type="OMA" id="NIKTGGM"/>
    <dbReference type="OrthoDB" id="5627at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DRT5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ97806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="272" mass="30469" checksum="4F53A53A59BD39C7" modified="2011-05-31" version="1">MVKNFRKRSLESDAADNSDDEDTRRVALEEIRYMQKLRERKLGIPAASVATGAASAAGATDGSSARGRGGSGAGAAGEEDLVLQDTFAQETAVTIEDPNMLRYVENELLKKRGKAIEVNDKDDKDQVDELYVVPDHLKVRKKNMEESSTQWTTGIAEVQLPIEYKLRNIEETEAAKKMLQERRLAGKTKSDANIPSSYSADFFHRGRDYAEKLRREHPELYKGQDSQANETGGKPTDSNNPGGPPAAHREAATDELLLERFRKREKFRVMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F2DVM2</accession>
    <name>F2DVM2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123425902</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AK367940">
        <property type="protein sequence ID" value="BAJ99143.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965601.1">
        <property type="nucleotide sequence ID" value="XM_045109666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DVM2"/>
    <dbReference type="SMR" id="F2DVM2"/>
    <dbReference type="PaxDb" id="4513-MLOC_79572.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141400"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141400.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141400.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141400"/>
    </dbReference>
    <dbReference type="GeneID" id="123425902"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0116450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0116450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0116450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0141400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0141400.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141400.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425902"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_37_4_1"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DVM2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000056">
        <property type="entry name" value="calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="85"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="385"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="421"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="457"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="495"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAJ99143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="532" mass="59377" checksum="AFF8EA140A53DD3C" modified="2011-05-31" version="1">MGQCCSRATSPDSVQGGANGYGYSHQPKQAQTPPSYNNAQPPPQAEVRYTPPAMNPPVVPPVVAPSKPTPDTILGKQYEDVRSVYSLGKELGRGQFGVTYLCTEISTGRQYACKSISKRKLVSKADKEDIRREIQIMQHLSGQPNIVEFCGAYEDKGSVHVVMELCAGGELFDRIIAKGHYSERAAATICRGVVNVVNVCHFMGVMHRDLKPENFLLATKDENAVLKATDFGLSVFIEEGKMYRDIVGSAYYVAPEVLRRNYGKEIDVWSAGVILYILLSGVPPFWAETEKGIFDAILQGEIDFESQPWPSISESAKDLVRKMLAQDPKKRISSAQVLQHPWLREGEASDKPIDSAVLSRMKQFRAMNKLKKMALKVIASNLNEEEIKGLKQMFMNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMDAADVDGNGSIDYVEFITATMHRHKLERDEHLFKAFQYFDKDNSGFITRDELETALIEHEMGDADTIKDIISEVDTDNDGRINYEEFCAMMRGGMQQPIRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXS1</accession>
    <name>F2DXS1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402274</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368689">
        <property type="protein sequence ID" value="BAJ99892.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952109.1">
        <property type="nucleotide sequence ID" value="XM_045096174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXS1"/>
    <dbReference type="STRING" id="112509.F2DXS1"/>
    <dbReference type="PaxDb" id="4513-MLOC_21213.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592540"/>
    </dbReference>
    <dbReference type="GeneID" id="123402274"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0491140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0491140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0491140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402274"/>
    <dbReference type="eggNOG" id="KOG3455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114589_1_0_1"/>
    <dbReference type="OMA" id="VEYLVYH"/>
    <dbReference type="OrthoDB" id="626714at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DXS1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF24">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ99892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="15500" checksum="B677BE4409A1B96C" modified="2011-05-31" version="1">MSATGRKLAALPALGWWLVVVGAVRLVFAWSGFFDAWAVRSGTYSNTHVTDVHARTVGVWTLLSCTLCFLCAFNLENKPLCAATLLSFVYAYGHFVAEYLVYRTITAASLGTLGFFAVPSIIWTVLHWRNTLGYRATKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2E3S4</accession>
    <name>F2E3S4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123410731</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="1">
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            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
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                <person name="Amano N."/>
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                <person name="Fiebig A."/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:IMP salvage"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
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        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="145"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="707"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
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        <ligand>
            <name>substrate</name>
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        <location>
            <begin position="488"/>
            <end position="493"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
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        <location>
            <position position="688"/>
        </location>
        <ligand>
            <name>substrate</name>
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        </location>
        <ligand>
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            <end position="766"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
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    </evidence>
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            <dbReference type="EMBL" id="BAK03119.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="865" mass="97428" checksum="BC9C508798B0EB3F" modified="2011-05-31" version="1">MDSASASYKLQLACAALVGASAAAASAYYLHCRAVAQLSGDIIRNSSAAAKGGRQRRRPRDAAGSNPPPPRRAAAGSSSLPDLSSIYAAGGVARGYPVGEEYDDGDEEGVGPYVDEALAAAAAYMQIPTGLPRLQVGPEANKKIVRSGSSRRVGLIRPNSPKSPVASASTLESAEGSDEDVVTKANGKSDNGYVNTNGNLEGEHKGNSVVENGAATPLAAKNMTRSHSISNDLHAVQPDPVAADILRKEPEQESFIKLLTAPHEIPSPDEIEVYKILQKCLELRDCYLFREEVAPWEKEVINDPCTPKPNPNPFTFVPEPKSEHVFQMVDGVVQVYADKDCTERIYPVADATTFFTDLHYVLRVTAAGNTRTVCHNRLNLLEHKFKFHLMLNADREFLAQKTAPHRDFYNVRKVDTHVHHSACMNQKHLLRFIKSKLRKEPDEVVIFRDGTYMTLKEVFESLDLTGYDLNVDLLDVHADKSTFHRFDKFNLKYNPCGQSRLREIFLKQDNLIQGRFLAELTMQVFSDLNASKYQMAEYRISIYGRKQSEWDQLASWIVNNELYSENVVWLIQIPRLYNVYKQMGIVTSFQNLLDNIFLPLFEVTIDPASHPQLHVFLKQVVGLDLVDDESKPERRPTKHMPTPEEWTNVFNPAFSYYAYYCYANLYTLNKLRESKGMNTIKFRPHAGEAGDVDHLAATFLLCHSISHGINLRKSPVLQYLYYLGQIGLAMSPLSNNSLFLDYHRNPFPMFFQRGLNVSLSTDDPLQIHLTKEPLVEEYSIAASLWKLSSCDLCEIARNSVYQSGFSHALKAHWIGKNYYKRGPSGNDIHRTNVPTIRIEFRDLIWRDEMQLVYLDNVILPDEVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F2E7W9</accession>
    <name>F2E7W9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123403180</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Putative FAD-dependent oxidoreductase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK372243">
        <property type="protein sequence ID" value="BAK03441.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953069.1">
        <property type="nucleotide sequence ID" value="XM_045097134.1"/>
    </dbReference>
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    <dbReference type="SMR" id="F2E7W9"/>
    <dbReference type="STRING" id="112509.F2E7W9"/>
    <dbReference type="PaxDb" id="4513-MLOC_6145.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559990"/>
    </dbReference>
    <dbReference type="GeneID" id="123403180"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0465230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0465230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0465230"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403180"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019845_2_0_1"/>
    <dbReference type="OMA" id="WRSKYEK"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="apoptosis-inducing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="8"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK03441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="357" mass="38695" checksum="7F041625F21A2FD0" modified="2011-05-31" version="1">MAEAGKPRVVVVGGGIGGALLAKTMEPDADVVLLDPKDYLEITWAELRSTVEPSFAERSLIYHRDYLTTATIVTSSAVNITENAVLTADGQSLAYDYLVVATGHVFASAGSRKERLTEFQRDNGKIKSSGSVLIIGGGPTGVELAAEIAVDYPEKKVTLVHRGSRLLEFVDQKASKKCLDWLTLKKVDVLFQQSVDLKSLSDTEKFYKTSGGETITADCHFVCIGKPLSSSWLHDTILKESLDTKGRVMVEKDLRVKGHDNIFAIGDITDIPEIKQGYLAQKHALLVAKNLKLLIKGSPPSKLATYSTGFPLAIVSLGRKEGLAQLPYLTLTGCIPGMLKSKDLFVGKTRKQMGLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F2E8H3</accession>
    <name>F2E8H3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123443503</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK372447">
        <property type="protein sequence ID" value="BAK03645.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975824.1">
        <property type="nucleotide sequence ID" value="XM_045119889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E8H3"/>
    <dbReference type="FunCoup" id="F2E8H3">
        <property type="interactions" value="1025"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2E8H3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0256390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0256390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0256390"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0256390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0256390.2"/>
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    </dbReference>
    <dbReference type="GeneID" id="123443503"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0256390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0256390.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0256390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0256390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0256390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443503"/>
    <dbReference type="OMA" id="TLWENNW"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007834">
        <property type="entry name" value="DSS1_SEM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771">
        <property type="entry name" value="26 PROTEASOME COMPLEX SUBUNIT DSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771:SF17">
        <property type="entry name" value="PROTEIN DELETION OF SUV3 SUPPRESSOR 1(I)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05160">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK03645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0256390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8697" checksum="925F80113376A0D2" modified="2011-05-31" version="1">MAAAAPADAKAEAAKMDLLEDDDEFEEFEINQEWDDKEDGNEAVQQWEDDWDDDDVNDDFSLQLRKELEGGNTQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2E9L9</accession>
    <name>F2E9L9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123425461</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK372844">
        <property type="protein sequence ID" value="BAK04041.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965101.1">
        <property type="nucleotide sequence ID" value="XM_045109166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E9L9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128860"/>
    </dbReference>
    <dbReference type="GeneID" id="123425461"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0106310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0106310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0106310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425461"/>
    <dbReference type="HOGENOM" id="CLU_124519_1_0_1"/>
    <dbReference type="OrthoDB" id="204784at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF244">
        <property type="entry name" value="OS07G0593400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK04041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="15469" checksum="D07C045CDEFB8304" modified="2011-05-31" version="1">MVSFDMNDRKKIGLGLTGFGVLFSFLGILMLFDKGFLAMGNILFVSGVSLTIGLKSTVQFFTKPKNHKGSIAFGIGLFLVLIGWPVFGMMAESYGFAALFSGFWPTAAVYLQKNPTVGWIFQHPYVTSLLTRFRGRRVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>M0UII2</accession>
    <name>M0UII2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123444454</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977103.1">
        <property type="nucleotide sequence ID" value="XM_045121168.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977109.1">
        <property type="nucleotide sequence ID" value="XM_045121174.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977118.1">
        <property type="nucleotide sequence ID" value="XM_045121183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UII2"/>
    <dbReference type="SMR" id="M0UII2"/>
    <dbReference type="PaxDb" id="4513-MLOC_11260.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070290.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070290.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070290"/>
    </dbReference>
    <dbReference type="GeneID" id="123444454"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0070290.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070290.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444454"/>
    <dbReference type="eggNOG" id="KOG2151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UII2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="475"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="57"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="223"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="390"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070290.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="612" mass="65249" checksum="ACEBB4E6BFEF875C" modified="2013-04-03" version="1">MSGSLNSNIGGDSTGRPFTSTFSGQSGSFPNFHHSGLHNIHGNLNLASLSPRNASLSGIPSPGVQQPGGNISGSRFPSNNLPVSMSQISHGHSGISNRGGMNVGGNPVFSSSMNAIGGSAQGLSSSMANVGNRNSAPGLTASSVLGNLGPRITNSAGNIVGGSSIGRSISSAGMSMPGISSRINSSGNSGSGALNIPSNRLSSMHQASPQFMNILGSSYPAQGGTLSQNQVQAGNNSFSSSGMLHDGNSGDNSPFDINDFPQLTGRPNSAGGGQGQYGSLRKQGVSVNAIVQQNQEFSIQNEDFPALPGYKGNSSDYGMDLHHKDHLHENANIMQAQHFPMGRSSGFNLGGSYPPRQQQQQSTTSVQNGLDNIGLRPINSPSPSSNSGSYEQLIQQYHQPQTQNSLRSQASSGQQSYKDQSQKSAQGKETVPDPYSLLGLLNLIKSKEPGPTALALGLDLTTLGLNLNSPDCLWKTFGSPWSNEPAKGEPDYQIPACYSAEQPPPLQPFNFPKFHPLTLFYIFYSMPKDVAQLYAANELYNKGWFYHREYRVWLTRASNVAPLVKTPLYERGSYICFDPNLWDTIQKDNFVLQYESVEKRPVLPSASQNVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>M0W417</accession>
    <name>M0W417_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123413601</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962473.1">
        <property type="nucleotide sequence ID" value="XM_045106538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W417"/>
    <dbReference type="SMR" id="M0W417"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0639930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0639930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0639930"/>
    </dbReference>
    <dbReference type="GeneID" id="123413601"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0531740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0531740.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0531740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0639930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0639930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0639930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413601"/>
    <dbReference type="OrthoDB" id="751756at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W417">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF17">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="5">
        <location>
            <begin position="598"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0639930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="727" mass="81704" checksum="1D8FA466C1AC4D9B" modified="2013-04-03" version="1">MVNADGEGADAGGGDPMCVDENAGAVVGADQCEEAGPQGGGAPVGLEGTSGEGGQVPVSHPGGGMRVRGATVEEESEEAPTVQGSKEGAEELLLKVVYSEEEAYKLYCDYGHHTGFSVRKGKQSYFTGTKRIRTKDYFCSKEGLKEAEKLTDENFNDPHTRTNCRAMVRFRVNSQGEWRVIRLVSDHNHNLVRPEERHLLRSAKSLIAGRSCSAADAVLYGGYQLGGVPSQMAASTSVTKNAEAKQDLLPGFSGVTRTSAVGTGELQSIVSHLKSRANVDGMFYWDVHLDRAGRMSNFFWRDGRCRMDYDCFGDVVVFDSTYRLNKQNYIVAPFVGVNHHWQTTMYGCALLADESVSSFTWLFKSFLEAMGNRQPRSIFTNQDQVMSNAIEEVFPNTRHRIAHWHIQKNAASRLGALNASKAFNKMFTKCMQGCDSESEFEETWTAMLREFKLQDNKWLSKLYKLKQKWCGALNKCTFDGGVENEPQCDSLSNIFTCIVDKSTSLSTIAAAVDKLTEDWREKEFDEDVRCCQRPLSCIIKHSDILNHAAKVYTHRIYKLFETYFLDGCGATKFKALPCEDSETYRFEMTMQGRGSRVCTVHLNMSTMQLTCSCSQFETMGLLCPHTLKALSIKNVGKIPEIYILKRWTRDAKQWLFNPKQYESSYQECMDDETGYCNHAMRYAYDLVTKSEGQEELRRSLWEALEGGEKELEKYLENATQYAQSYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>M0W4I8</accession>
    <name>M0W4I8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444646</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977371.1">
        <property type="nucleotide sequence ID" value="XM_045121436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W4I8"/>
    <dbReference type="SMR" id="M0W4I8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0295670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0295670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0295670"/>
    </dbReference>
    <dbReference type="GeneID" id="123444646"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0246160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0246160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0246160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0295670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0295670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0295670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444646"/>
    <dbReference type="OMA" id="EWIFYDA"/>
    <dbReference type="OrthoDB" id="775811at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W4I8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="191"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="432"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein RPS4B/Roq1-like leucine-rich repeats" evidence="9">
        <location>
            <begin position="573"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="11">
        <location>
            <begin position="678"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0295670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1117" mass="126942" checksum="A6A022613C0BD8AA" modified="2013-04-03" version="1">MAAEAILGAFMQTLFQKLSEALLDHFKSCRGIHGKLESLSQILSQLQAFLDDAEAKQLADASVRGWLAKLKDVAYDLDDLLDRYTAKIMYLKKRKVKLSTKASVGSPSSFLRKNMCQYRIKRKISGILERLDKIARERDTIGLQMLGEMSRRETSERPQSSSLVDGSALFGREGDREEMVRLMLSENGHTSCNVCVIPVVGMGGLGKTTLMQMVYNDERVIEHFELRIWIYVSECFDGRKLTQETLEAAAYDESFPSTNMNMLQETLSRVLRGKRYLLVLDDVWNEDYDKWLSYRAALISGGLGSKIVVTTRNDNVGRIMGGIEPYKLQQLSDDDSWSVFKNHAFRDGDCSTYSQLEVIGRQIVKKLKGLPLASKALGSLLFCKADEEEWKGILRSDIWELPADKNNILPALRLSYNHLPPHLKQCFAFCSVYPKDYIFSKEKLVKIWLALGFIRQSRKKILEDSGNAYFHELASRSFFQPYKENYVMHDAMHDLAISVSMEHCERFEDGTRYDNAIKTRHLSFPCTSVRTRHFDPLYGFKKLRTLILMHGYNSKMSRFPDGVFMKLQFLRVLDMHGRGLKELPESIGNLKQLRFLDLSSTEIKTLPASIVKLYNLQILKLNNCSSLREVPHGITKLTNLRHLEGSTRLLSRIPGIGNLICLQELEEFVVLKRSGHNITELRNMDQLQGKLTIRGLNNVADEQDAICAKLKTKEHLRALHFIWDEDCKLPPSDQQDEILEGLQPHIDLKELMIKGFPGARFPSWLATSFLPNLQTTHICNCRCTVLPPLGQLPFLKNLNIAGATEVTQIGHEFTGLGQIKCFPALEELLLEDMPKLGEWIFDDADQLFPQLTELGLINCPKLKKLPPVPPTLTTLRIDEAGLESLPDLQKEACPSSLTFLYINGCPNLTSLRVGLLSHNLTALKSLTVAHCEELVSLPEECFRPLTSLQFLHIYECPCLVPWTALERGLLPASVEEIRLTSCSLLAHVLLDGLQYLPRLKHFQIADYQIADYRDISNFPLELLPHTLQFLDISRCDDLQCLPPSLHKVSSLETLHISNCRELESLPEEGLPRGLKELYINQCPKIKQRCQEGGMDRVKIAHIRDIEIDGDVIVLEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>M0XJN9</accession>
    <name>M0XJN9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123443434</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044975737.1">
        <property type="nucleotide sequence ID" value="XM_045119802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XJN9"/>
    <dbReference type="SMR" id="M0XJN9"/>
    <dbReference type="FunCoup" id="M0XJN9">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XJN9"/>
    <dbReference type="PaxDb" id="4513-MLOC_6066.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254600"/>
    </dbReference>
    <dbReference type="GeneID" id="123443434"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0211410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0211410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0211410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0254600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443434"/>
    <dbReference type="eggNOG" id="ENOG502QYZR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XJN9"/>
    <dbReference type="OrthoDB" id="623685at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XJN9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000002">
        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF235">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="482" mass="53326" checksum="3E80496E9FE58BA4" modified="2013-04-03" version="1">METLRAMALPPGFGFHPKDTELVAHYLKKKILGQKIEYDIIPEVDIYKHEPWDLPAKCNVPTQDNKWHFFAARDRKYPNGARSNRATVAGYWKSTGKDRAIKVDKRTIGTKKTLVFHEGRPPTGKRTEWIMHEYYIDENECQACPDMKDAFVLCKVTKRIDWTSENGNEVGNNNPQPQQPNVAAILAVSVEQPDTAGSSIIGGELPSDAATVAIPAHTTPDGDADIQQWLEELIDPSFDPSANTVVDSVSAQLSPDEQNAESSNIDAMAPKVEQDYASPNQTLADDTDFLLSDDIHSMLYPGSDDFTSWQHTYFTAADPFSLSNDFMDEFQMKELQLPLENNGPNLSNEPAVVVRTRHGGTSATSVPPYKAKARLQQRLGRMVTSSSESINQTIKFVDNNGHLDLMTNVKHQKKHVRDITSVKQSDAGKSSGNHNNQGFFRGVQRAFRGCSATGLNILVALCMVGVAAAILHHGRHRSGISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287QE55</accession>
    <name>A0A287QE55_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123398822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949206.1">
        <property type="nucleotide sequence ID" value="XM_045093271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287QE55"/>
    <dbReference type="SMR" id="A0A287QE55"/>
    <dbReference type="FunCoup" id="A0A287QE55">
        <property type="interactions" value="960"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287QE55"/>
    <dbReference type="PaxDb" id="4513-MLOC_76109.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432300.1"/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127">
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        <property type="match status" value="1"/>
    </dbReference>
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        </location>
    </feature>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
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            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="718" mass="79765" checksum="8988460F05855F94" modified="2022-05-25" version="1">MAKAQQPPWVVQFNGTSKPVLVAPLDGCRRSDETPDMPVLQGKRCLGVDGGWSLMLDEVTNGCSLVSIADDDASPSSPTVIALPPFPEEVPKQLLFSCALSSQTPPNCTVVLSFFTGMTSLIHCRPGDPGWSRLPVDLPEEGDEFDGPITADRQGKVYATTMVSLVAADASSPTLAVERTDMWHPPSCPAHAGYKCYPVACPSGDLFLVRCCNFGYPSEVVDLKVFQWNRDENAWETVETIGDRTFFVGMFSFAVPSASEAGTQPNCIHMLHRDCGESGVYTMSLDNMTIMLSVVEGCGDDQLFWALPTSFGLKAARSIDIPADVSNEVIQRRTAHCRREEEQEGTAASQEERQWGELDTDLLQLLVSKMSLSDRIHINVVCKQWNSNNSPIQDAKVSPLLMRIRSTGRTKEDSLEIFDPVSDKKYIIRVDIPVSGLKSRTSHLLHFTKNGWVIMSRGGDHMFFLVNPFKNCSDGGHVIALPPLDVLGLKGLSFSSVPGSSDFVVLAAGSTPSGKVVIVNTWRMGDEEWKEECLSDDDAPFFMASHSPVFLDGVFYFLDINGRLGVVDPNEDEMEFRVLKKPDQPIRGSDEVHLRERECNYLVEWKGELIAIFRKNANGSVRMFKLDRSQMLWTELQGIDDATVFWDRCNALVAVPPAGEDLCNKMILPNYKETEGGGRTHAFYSFQEQCYYPSFCVKEPMNAIWFQPNLDVFTVTRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGS7</accession>
    <name>A0A8I6WGS7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123440285</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044972801.1">
        <property type="nucleotide sequence ID" value="XM_045116866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGS7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059810"/>
    </dbReference>
    <dbReference type="GeneID" id="123440285"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0047480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0047480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0047480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0059810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440285"/>
    <dbReference type="OMA" id="PWKQYFS"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12361">
        <property type="entry name" value="RRM1_2_CELF1-6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12362">
        <property type="entry name" value="RRM3_CELF1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000302">
        <property type="entry name" value="RNA-binding protein BRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000334">
        <property type="entry name" value="RNA-binding protein BRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000216">
        <property type="entry name" value="RNA-binding protein BRN1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="40"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="128"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="374"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="321"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="460" mass="49343" checksum="FCB79ABD43C6E2AE" modified="2022-05-25" version="1">MAEDAGDREQQQPAAAACAGAVGAGHACGEDGGGRDESSVKLFVGQVPKLMTEAELAAMFRDVAIVDEVTVIRDKATKASRGCCFLICPSREEADKAVNAYHNKRTLPGAPSPLQVKYADGELERLEHKLFIGMLPKNVTDVEMTDLFSQYGNIKDLQILRGSQQTSKAGCAFLKYETKEQAVAAIEALNGKHKIEGSSVPLVVKWADTEKERQARKAQKAQYPPSNMSNANAMQQSSLFGALQMGYVPPYNGFGYQPQGTYGLMQYPLSPMQNQAGFQNMVQPVNQGSSIRGVSSELSPNSVARSFNSMQLGSPYPAGPGMQYPGSYPGGGINSRPYMNSHNSVKVPNANATSPTSSSTGSNTGPQLEGPPGANLFIYHIPQEFGDQDLAHAFQSFGRVLSAKVFVDKATGASKCFGFVSYDSPAPAQAAISMMNGFQLGGKKLKVQLKRDNSKHNKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJV3</accession>
    <name>A0A8I6WJV3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphotransferase</fullName>
            <ecNumber evidence="12">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123412023</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_044960914.1">
        <property type="nucleotide sequence ID" value="XM_045104979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJV3"/>
    <dbReference type="SMR" id="A0A8I6WJV3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059550"/>
    </dbReference>
    <dbReference type="GeneID" id="123412023"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0047240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0047240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0047240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0059550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412023"/>
    <dbReference type="OMA" id="HYDQAFE"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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    <sequence length="500" mass="54735" checksum="7FA1FFBB0D8922D9" modified="2022-05-25" version="1">MGGAGWLRVAAAAGCAAVTCTLATTMVVRRVTAWRRWLRAMAVLRDVEESFATPTERLQRVVNSLAIEMFAGLASEDASKVRMLLTCVDSLLDGSEEGIYYAIDIGGTSFRFLKVELGAGSTIINQKIEHQPIKKELMEGTSEDFFSFIASTLKSFIEREGGEGRALGFTFSFPMRQFSITSGSLIRWTKEFSIEEAVGKDVAQCLNEALERNGLNLRVNALMNNTVGTLALGHYYDEDTIAAVIIGAGTNACYIERNEAITKCQGLLTNSGQTVVNVEWGSFRPPQIPLTPYDICFYNETPNYYDQGFEKMISGVYLGEIARRVFHKMAQESDVFGTNVDGLSIPFILSTPCLAAIREDDSLDLKEVGRILEEHLKIHNIPLKTRKLVQRVCDIITQRAARLVAAGIVAVLQKLGRDGTLCGTTRVRSITGVPKRSVIAIEGGLYQGYSVFREYLDEAILEILGEEIAATVVLKVVEEGSGMGAALIAAAYSSTRKNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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</entry>
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    <reference evidence="5" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
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            <end position="101"/>
        </location>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <sequence length="101" mass="11177" checksum="72FC4B3E78675AE6" modified="2022-05-25" version="1">MLESRVFCPRWISLIKCLLFQGTFTVRINDTTGPYFVGGKGIKQGGPISQLLFNLVADVFSKMLTKATRHRLISGLLTNVIPGGIISLQYADDTLLFIENS</sequence>
</entry>
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    <accession>A0A8I6WLV8</accession>
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            <fullName evidence="7">Myb-like domain-containing protein</fullName>
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        <lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">cv. Morex</strain>
        </source>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A8I6WUD9</accession>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0297840.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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        <lineage>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045129040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WVU5"/>
    <dbReference type="SMR" id="A0A8I6WVU5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093760"/>
    </dbReference>
    <dbReference type="GeneID" id="123452389"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0077220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0077220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0077220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0093760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452389"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA-ATPase ASD, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="235"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="446"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="446"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="506" mass="57448" checksum="35EFEF4193F5BCD0" modified="2022-05-25" version="1">MAPSYDKTIATAASLAASLMLVRSLASELLPSEVRGALSTALSSLRARMTWQHTIVIEENEGWSSNRVYSAVKAYLATRINANINMQRLRVSSTDESEKMVVSMEAGEEMADVYQGAEFNWCLVTHEVSGDPNNGGGGAREVRSYEVSFHKRHKEKALKEYLPFIVATAKAIKDQERSLNIYMNERYDEWSPIDLQHPSTFDTLAMDQKQKQSIVDDLDRFIKRKDYYRRIGKAWKRGYLLYGPPGTGKSSLIAAIANHLRFDIYDLELTGVNSNSDLRRLLVGMTNRSILVVEDIDCTIELKQREEDDEEDSKSNSTEKKPEDKVTLSGLLNFVDGLWSTSGEERIIIFTTNYKERLDPALLRPGRMDMHIHMGYCTTEAFRILANNYHSIDYHATYPEIEELIEEVTVTPAEVAEVLMRNDDTDVALHDLVELLKLKKNDATEIGTESKKAEEKKDSNEIKTQSMQVDEKKTVDEIKTESVQVEEKKDDKKVVVKNDFTENGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WW92</accession>
    <name>A0A8I6WW92_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6WW92"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226140.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0187710.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00011">
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    </dbReference>
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        <property type="entry name" value="HSP20-like chaperones"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
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        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="99" mass="10871" checksum="D9D218C56D9C3F12" modified="2022-05-25" version="1">MSIVRRSNVFDPFADLWADPFDTFRSIIPAISGNSETATFANARMDWKETPEAHVFKADLPGVKKEEVKVEASSAASAVRRRRTRMISGTVWSAAAAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X071</accession>
    <name>A0A8I6X071_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X071"/>
    <dbReference type="SMR" id="A0A8I6X071"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0249660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0249660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0249660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0249660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0249660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0249660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0249660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="27026" checksum="E3D0098D025EE530" modified="2022-05-25" version="1">MATFILVKFTEFCQPDATMPFGKELVQITKDLRIALPTITGKPTLSYPEDSRYRIEVHVPGRTFEPRTEPVDFKFIAPNWILGRDMAIHCALGRIKEEYKGFPISPELFTVSRRSKDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLRELELEEAAREAHYEHELEVKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPITMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2S4</accession>
    <name>A0A8I6X2S4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fucose_pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEVGALKINASE"/>
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        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="137"/>
            <end position="549"/>
        </location>
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        <location>
            <begin position="825"/>
            <end position="895"/>
        </location>
    </feature>
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            <begin position="976"/>
            <end position="1055"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038121"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08544"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="11">
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1073" mass="116496" checksum="55149EB0D50C5FB5" modified="2022-05-25" version="1">MEPGHRRRRRAHTADEAAAVLRKAWCRLRLSARDPARVPPWDAVVLTAASPEQAALYDRQLARARRLARFPPSTAALAVPDPDGARIGSGAATLHAVASLVRHLLSQATKEEIAEFLPEATGSSADDVPLASVVRFMATKHVLLLHAGGDSKRVPWANPMGKAFLPVPYLAGDNPDGPVPLLFDHILAVSASARQAFKNQGGIFIMTGDVLPCFDASNLLLPDDAACIVTAPTTLDVASNHGVVVASKDGTEGENYSLCLVDDLLQKPTVSELVEGQAILDDGRALLDTGIIAVRGKAWQELVALAYSSSQTMIKEIITSRKELSLYEDLVAGWVPAKHEWLRNRPFGKELIAALGRQKMFSFCSYDFSFLHFGTSAEVLDHLAGSYSGLVGRRHMCSVPETTACDIAATTVILCSRISAGVSIGEDSLVYDSSLSGRVRIGSQSIVVGVNIHELHHGNSPQIMSSSTCFTLPDRHCLWEVPLVNSMGRVMVYCGLHDNPKVSMKRDGTFCGKPWKNVLEDLKIQDTDMWDTSNLDKCLWNARLFPIMSPPEMLNVGMWLMGSGRDPDGKVSCMWRKSRRVSLEELHRSIDYHQLCRDSAKHQADLTAAVAKSCVTYGLLGRNLFQLCEEMLGNDSSSVEVCKELLTFFPSQGDQYPGALPQSRGYQVKSDLLRASGDLSTASMVEEKVWASVASETASAIKYGSKEPSSSATTSSNGNLRPKKAVVELPVRVDFVGGWSDTPPWSLERPGCVLNMAISLEGRLPVGATIEATEDHHGVLVEDDAARKVYIEDLSSISCPFKEDDPFRLVKSALIVTGILGHEMLSTAGLKIRTWAKVPRGSGLGTSSILAAAVVKCLFQLMEDDGGDDNVARAVLVVEQIMGTGGGWQDQIGGLYPGIKCTQSFPGQPLRLQVVPLLASPQLIQELEQRLLVVFTGQVRLAHRVLEKVVTRYLRRDSLLISSIRRLAELARAGREALMNGEVDELGGIMLEAWGLHQELDPFCSNKLVDELFALADPYCCGYKLVGAGGGGFALLLARSPGHAVELRRALRDSMAGLDVTVYDWNVAVPLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6M4</accession>
    <name>A0A8I6X6M4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6M4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="20715" checksum="CF6E12019AEA32D4" modified="2022-05-25" version="1">MWHYIGPEDSTRTNVVGVPEDRVTSWVLHITGPCENPKGARRVKPFSADHPPPNQEWTNWFSPASNGNPEEEEEEGSQEGSVESAEYVSDSGESEEEDSEEEEEDEERDSPPPRPEHRSKRQHEPAVPSAPPASSSAPPAAPVAPSAQGTKRSRDASAEPAGQSSRAPKPSGPKPRKALPRMRVAIPVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8C7</accession>
    <name>A0A8I6X8C7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123440005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972534.1">
        <property type="nucleotide sequence ID" value="XM_045116599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8C7"/>
    <dbReference type="SMR" id="A0A8I6X8C7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0274270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0274270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0274270"/>
    </dbReference>
    <dbReference type="GeneID" id="123440005"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0274270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0274270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0274270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440005"/>
    <dbReference type="OMA" id="CPDANHR"/>
    <dbReference type="OrthoDB" id="647970at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035175303" description="Thaumatin-like protein" evidence="3">
        <location>
            <begin position="27"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="95"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0274270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="18355" checksum="107A7933F2D3AA4E" modified="2022-05-25" version="1">MATPAATSLALAVGLLLVAFTAGAGAATFSITNRCPFPVCAAAMPVSGGGGAQLAPGETWTLTFAAGGTAGRIWPRTGCSFDPATGRGGCQTGDCGGALRCVLSGKPPATLAEFNLANGGAPGRYGISVVDGFTLPMDFSCSGAGDGGVIRCRDPGCPDGNHRPGDGKYRACPAYRDYQVVFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8I2</accession>
    <name>A0A8I6X8I2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8I2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0437770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="91" mass="10358" checksum="D19BCCF02EF76836" modified="2022-05-25" version="1">MDVKTAFLNGELDEEIYMEQPDGFVVDGQEGKVCKLLKSLYGLKQAPKQWHEKFERTLTTAGFVVNETDKCVYYRHGGGEGVILCLYVDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAS7</accession>
    <name>A0A8I6XAS7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965133.1">
        <property type="nucleotide sequence ID" value="XM_045109198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAS7"/>
    <dbReference type="SMR" id="A0A8I6XAS7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0120190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0120190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0120190"/>
    </dbReference>
    <dbReference type="GeneID" id="123425504"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0098960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0098960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0098960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0120190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0120190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0120190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425504"/>
    <dbReference type="OMA" id="WNAYQDE"/>
    <dbReference type="OrthoDB" id="742322at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0120190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="374" mass="42109" checksum="B63C11E563016387" modified="2022-05-25" version="1">MNIDRFHMAKGEGETSYVNNSRIQHKALLETEAVLEKAVREVFKYLHHPTMTAVDLGCSSGQNTLTFVSKVIQVIGRDIGGESGRNPVELQFFLNDLPGNDFNHVFGSLERFKELTAAEHKENTLVPSFYIAGLPGSYYTRLFPRQSCHLFHSSFCLHWRSRVPAGLEGGGRKYLNEGNIYIARTTPAGVAELYRRQFQNDMLLFLKLRYEELVVGGQMVLTFLGRKYEDIYNKGYLNHPCGLLAQSLQSLVEEGLVEKEKLDSFNLPIYTPSINEVKEVVAQSELFNISHIKLFESNWDPHDDSQGDDAHNTVQSGINIAKSLRAVLGPLLASHFGESLLDEIFKKCAYCVTEHLVERGEGNYLLICVSLKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XB53</accession>
    <name>A0A8I6XB53_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123440788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 10">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044973271.1">
        <property type="nucleotide sequence ID" value="XM_045117336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XB53"/>
    <dbReference type="SMR" id="A0A8I6XB53"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313640"/>
    </dbReference>
    <dbReference type="GeneID" id="123440788"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0313640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440788"/>
    <dbReference type="OMA" id="MKNHIVD"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009635">
        <property type="term" value="P:response to herbicide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF32">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01154">
        <property type="entry name" value="Main.5:_Phi-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="92"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="223" mass="24194" checksum="8CC751361B90CE77" modified="2022-05-25" version="1">MAEPPPATAVSRKLYGYALSSNSVRIAALLNEKGLDYELVVVDDTKAPEFLAINPLGQVPAFQDGDDILFESRAISRYIATKYRSSGTDLLPATASAKLEVWLEVESHHFYPAVADLVYELRVRPRLPGGAPPDPAVVDGLAGKVADVLDVYDAHLAAGNRYLAGEQFTLADVNHIAQLFVMSRTPRAAELVAARPHVQAWWDEISARPAWKKTVAALPLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBQ7</accession>
    <name>A0A8I6XBQ7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBQ7"/>
    <dbReference type="SMR" id="A0A8I6XBQ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0143690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0143690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0143690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0173850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010167">
        <property type="term" value="P:response to nitrate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="562" mass="60236" checksum="0BCE5E0F067768C8" modified="2022-05-25" version="1">MVSTGGFDDNGDCEGLVVDFKGRPVDKSRVGGWFGAGLILVTELAERVCVMGISMNLVTYLTGDMHLSSAKSANIVTNFMGTLNLLALLGGFIADAKFGRYRTIAVAATITAAGVSLLAASTAVPGMRPPRCAGGEARPRAAGCVPASGGQLGALYGALYTIAAGAGGLKANVSGFGTDQFDGRDPREERAMLYFFNRFYFCVSLGSLFAVTVLVYVQDNVGRAWGYGVSAAAMAAAVAVFAAGASRYRYRRPEGSPLAVIGRVLWGAWRNRRLPCPADASELRGFHEAKVPHTDRLRCLDKAAIVEADPATAVTVTEVEEVKMVVKLLPMWSTCILFWTIYSQMTTFSIQQAQQMDRRAGVRFVVPAGSLSVFLFLSVLLFTALNERVLVPLAARLTRRPQGLTSLQRVAAGLALATLGMAVSALVEKKRRDASNGSGGVAVSALWLVPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLATLAVGFFLSSLLVLAVDAVTRGAWIRGHLDDGRLDLFYWMLAVLGLANFAVFLVFASRHQYKRTRPDAATVELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCS9</accession>
    <name>A0A8I6XCS9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123443286</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975553.1">
        <property type="nucleotide sequence ID" value="XM_045119618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCS9"/>
    <dbReference type="SMR" id="A0A8I6XCS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0249790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0249790.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0249790"/>
    </dbReference>
    <dbReference type="GeneID" id="123443286"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0207440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0207440.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0207440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0249790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0249790.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0249790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443286"/>
    <dbReference type="OMA" id="APIMDSE"/>
    <dbReference type="OrthoDB" id="3268246at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000057">
        <property type="entry name" value="Exportin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0249790.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="981" mass="108086" checksum="F66594A3524E3AA9" modified="2022-05-25" version="1">MEVPPEMLDTLAGWFAQTLSPDAAARRAAEQSLAAAASTPGFALALLGLASSPRHDLQARLAASVHFKNLLRRRWPKPADDADDADHLPPNDCVIIKTHILQLLLTAPPLIQSQLSEALAAAAASDFPARWESLLPSIVSSLGNALSAGDVPATNSLLAAAVSLFSRFRNAFDSNTLRIDLKYCLDTFAAPLLEVFLSTSRRLQASAAAASPLEIRPVFECLRLCCEIFYSLNSVDLPEFFEDHMREWMTEFRAFLTTSYPPPVEADGAPDALRAAVCDNLQLYMEKYEEEFRAYLKEFVEAVWGLLMAPTVSPSRGQLAVTAIRFLTTVAESVHHALFGTPDAMKQICDSVVVPNLRLRDEDEELFEVNWVEYVRRDAEGSDTDTLRRAACRLLRGLAANYREQVAVLVSAQVQQMLAAYAADRANNWKEKDAAIYLVIALMQKPGATGGGTPVVDMESFFTSVIVSELQAPDWESEPMLKATVLRFLKEFKDQIPKATALALLPSVTRFLTHESNVVHSYAAIFIENLLITKDAVQVPGANVVTRAPRYVAADINSFAQQIIQSLSKALGYPDSYENPYLMKCLMRVLGIATIAGQVVHEITARLVGILMEVCNNPKNPDFNHYLFEALAAVIGKAGEQDPALVPLFEASLFPVLQRILVEDISEFWPYAFQIFAQLVNLSRPPLSQNYMQLFGVLLSNATWDRPPCVPALVRLLRAFLRKIPNELNQEGRLPNILVIFRSLVSRSSTEDSAFYMLNTLVENVGLDIINPHISEIWSALFTRLQTRQAVKFVNSLVVVMSLVSVKYGPGVLVSSVDTIQPNLFTTILQRFWIPNLKLIKGSLEIKLTAVASTKLLCESAVLLDAAAAQTWGKLLDSIVTLLSRTEQDGVQQEQNDGADAVDSQRTSGYSVSFVRLQYAGKSEDDLLKDINDPKQFLVTSLASLSAQSPGRFGPTIEQHVDPANKNALLQLCAAYNANIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XE07</accession>
    <name>A0A8I6XE07_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF177 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123430552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970348.1">
        <property type="nucleotide sequence ID" value="XM_045114413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XE07"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0182920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0182920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0182920"/>
    </dbReference>
    <dbReference type="GeneID" id="123430552"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0151330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0151330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0151330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0182920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0182920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0182920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430552"/>
    <dbReference type="OrthoDB" id="1912778at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044985">
        <property type="entry name" value="YceD_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37734">
        <property type="entry name" value="LARGE RIBOSOMAL RNA SUBUNIT ACCUMULATION PROTEIN YCED HOMOLOG 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37734:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL RNA SUBUNIT ACCUMULATION PROTEIN YCED HOMOLOG 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0182920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27573" checksum="F59E1F094C122139" modified="2022-05-25" version="1">MARACKCSPAVRLLPKPITAQLPGSRSHGRCRRFAVHAQLPTEDDYPAESPKKVQVTQGLKRSRRRGHGTRQSLVSVGTSRGGGDQWSSDFELTLRQLRLDDLIEDGQSDADVLVHLLVQQHAQFGMSIKGRVVTSLTKMCDSCSAPYCTKIDEQFDITVLSSSRKDQSGLPEIGDSDPSVIYVKPGTEIDIDSSIQETIRLTASAKSSCSEACEKSPVVWKRAGNQKKRYSQTWSKLLDLKRTLDKAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFF6</accession>
    <name>A0A8I6XFF6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123399195</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in plant defense signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044949563.1">
        <property type="nucleotide sequence ID" value="XM_045093628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFF6"/>
    <dbReference type="SMR" id="A0A8I6XFF6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480140"/>
    </dbReference>
    <dbReference type="GeneID" id="123399195"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0397870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0397870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0397870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399195"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="80"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="450" mass="49925" checksum="10192B100F62D567" modified="2022-05-25" version="1">MAAQSWNPFSCCVRGAAAEDDDDHCESRRGNNKGSPRSPLKNLCSSGTLSPEELSLTLSGSGSNLHAFTYTELRAATASFSRANYLGCGGFGPVYKGAVDDRLRPGLAAQAVAVKYLDLECGTQGHQEWLAEVFFLGQLRHKNLVKLIGYCYQNEHRMLVYEFMSAGSLENHLFKSINGSLPWMTRMKIAVGAAKGLAFLHGADPPVIYRDFKASNILLDSDYNTKLSDFGLAKDGPQGDATHVTTRVMGTHGYAAPEYIMTGHLTAKSDVYSFGVVLLELLSGRQSVDRARRPREQNLVDWARPYLKRSDKLHQVMDSTLEGQYSCKGAEVAALVAYKCLSQNPKSRPSMKEVVKALEPVLSMDDFFPVGPIVFTIVVEEDKVMDMKVEVEEKHQHHHRNHQDRHRKKYPESAIHGGIVLHGDNGHVAGFTGTLRRQQRTLSYHRERGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI19</accession>
    <name>A0A8I6XI19_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI19"/>
    <dbReference type="SMR" id="A0A8I6XI19"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0406800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035178236" description="3'-5' exonuclease domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="93"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="28953" checksum="E531EE8B0F99E0C7" modified="2022-05-25" version="1">MKSTNGSTVAISLFLLLSRSPSLLFSSLLPFSSSTMSSRFLKVLAHGTTKLNVVYTNESREVPFFLEQLKEKWFDDAMEHEKFLRLVLEYTADQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSGITFATVDIRNDKLKMRHNFGIEIPAGCLVDLQTVFRLRHDRTSMAHMAVALIDEEYGDMKTSFPKSQHKLWKKAPLDPINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGYSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK52</accession>
    <name>A0A8I6XK52_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442788</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974856.1">
        <property type="nucleotide sequence ID" value="XM_045118921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK52"/>
    <dbReference type="SMR" id="A0A8I6XK52"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233230"/>
    </dbReference>
    <dbReference type="GeneID" id="123442788"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442788"/>
    <dbReference type="OrthoDB" id="439710at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00777">
        <property type="entry name" value="AspRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04317">
        <property type="entry name" value="EcAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047089">
        <property type="entry name" value="Asp-tRNA-ligase_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047090">
        <property type="entry name" value="AspRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001750">
        <property type="entry name" value="PRK00476.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="231"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="655"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="676" mass="75162" checksum="29FD5DA73591BC71" modified="2022-05-25" version="1">MASSLLRASPLSLLSRLKPLSRRPPAPHLRGILLLSGSASASPRSTPTPRTLAAATDAATPPEEAAAAVLPAAEAKLERMQPLQWPQRDALCGELGAGDAGRRVRLCGWVALRRSHAGLTFLTLRDSSGMVQVTTLPEYPEVYAVVNKLRVESVVSVEGVVRLRPTEAFNADMRTGAIEIAADRVSVLNSVTRSLPFPITTADTVTERFPEEVRLRFRVLDLRRPQMQSNLRLRHKVIKHIRRYLEDRHDFVEIETPILSKSTPEGARDYLVPSRVQPGTCFALPQSPQLFKQMLMASGFEKYFQIARCFRDEDLRADRQPEFTQLDMEIAFTSMEDMLKLNEELMIHIFQEVGDIKLPKCFPRLTYTEAMNRYGTDRPDLRFDWELKDASDVFLGSSFKVFADTLENGGVIKALCVPGGAKVFSNTDLKKGTVYAEASKAGAKGLPFLKVMDNGELEGVGPLVSSLKPEKKEHLLELLDAKAGDLILFALGEQSTANQILGRLRLFIAHKLEVIDTSAHSILWVTDFPMFEWNSDEQRYEALHHPFTAPNPEDMNDLPSARALAYDMIYNGVEIGGGSLRIYKSDVQQRIFEIIGISPEQAEEKFGYLLECFDMGAPPHGGIAYGLDRLVMLLARESSIRDVIAFPKTTTAQCSLTKAPSPVDPQQLKDLGLRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKP0</accession>
    <name>A0A8I6XKP0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0436190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0436190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0436190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0436190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0436190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0436190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0436190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="98" mass="10916" checksum="BD24878352B15F91" modified="2022-05-25" version="1">MEQCRQGKSAKRIHNFGKRIGSEDWARGSRPRTCRLLADCSSCSRGESGSPRAGRVTDRESPLRELSPSMKQSTQNWYGQGESDCLIKTKHCDGPRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XN26</accession>
    <name>A0A8I6XN26_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XN26"/>
    <dbReference type="SMR" id="A0A8I6XN26"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0425750.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0425750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0425750.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF34">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303971" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="8">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="231"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0425750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="355" mass="36834" checksum="B5C7F30A6B6EF5A7" modified="2022-05-25" version="1">MASAAALLLAAAVVALLVCLALADPPASEQSALLAFLAATPHERKLGWSAATPACAWVGVTCDAANSTVIKLRLPGVGLVGPIPPSTIGRLTNLQVLSLRANRVSGAIPDDILRLSALRSVFLQDNAISGAIPPGVSGLAALERLVHSHNNLSGPIPFALAGLAALRALRLDGNRLSGKIPSIANPGLKVFNVSDNRLNGSIPRALSRFPADAFAGNLQLCGTPLPPCSPFFPSPSPAPPPGMGPSDGKPPKKKKKVPARRARRRRKRHGGRGDRHNKTAGVVRRRYRHGVLAKGRRRNLRVGGRGGGGRRDAGGEPAGVRASAEMLGKGSAGTSYKAVLEEGTTVVVEVSVSRR</sequence>
</entry>
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    <name>A0A8I6XNX8_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNX8"/>
    <dbReference type="SMR" id="A0A8I6XNX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0437550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF8">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="7">
        <location>
            <begin position="51"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="127"/>
            <end position="271"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="287" mass="33671" checksum="EF9318E3F9EBB7BF" modified="2022-05-25" version="1">MEFEEESSDDNNQDYSQMLYGNSEDDEKYEIHDSIEDEDYRGLELDLMVFCRKHGKATERFVAFEGTSSGRRFLACAEQGADNCGFVEWVDPYWPTPMQNALLKLWQMYEDAKAYRRSDNLNSALTIQTLTCEKKSLEDKFQELAKHVDTLFQAQETRTKEHLYVVSEYKKEFEEQKAEIARKEGENQKLNEKYVILENLSRAQGKMIKNVKCNHLKEKERLIEERRKMKLQISQLHEVLANSEAQIIDEVTKLKNELACMTLSNEKLTEEVSTSSSWNQLLNEKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XP26</accession>
    <name>A0A8I6XP26_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123442542</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XP26"/>
    <dbReference type="SMR" id="A0A8I6XP26"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0225290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0225290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0225290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0186970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0186970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0186970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0225290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0225290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0225290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000001">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000503">
        <property type="entry name" value="Cullin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000013">
        <property type="entry name" value="Cullin-1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000005">
        <property type="entry name" value="Cullin-1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000020">
        <property type="entry name" value="Cullin-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000021">
        <property type="entry name" value="Cullin-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="6">
        <location>
            <begin position="372"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0225290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="727" mass="84380" checksum="AFAF04319D6E26A5" modified="2022-05-25" version="1">MDAGVARLKRILHGVDGVSFTSQEYIHLYTTIFNMCTQKPPYDYSEQLYERYKQALEDYIKSTVLPALKSKHGEFLLRELVERWKNHKVMVRWLTRFFHYLDRYYVSRKALKPLKLLGVSCFYDLVFNGLKTTLTTILLDMIDNEREGQLIDRALVKDVIDIYLEIGQGGVDLYEPDFEQAFCKATTDYYSKKAQTWMLEDSCPQYMLKAEDCLQKEKERVAHYLHSSTEEKLLEGTMLELISKRAEEILNKENSGCRVLLLDGKSEDLSRMYRLFAKIEAGMSHVSKTFKEHVREEGMSLLKHAADAANGAKNERKETVGSPEQEFVRKVIELHDKHLAYVINCFQNNLIFHKALKAAFEDFCDKDVAGCTSAESLASFCDSILRKGGSEKLSAEVVEDTLDKVVTILTFISDKDLFVEFHRKKLGKRLLFDKSANNVHEQNLLSKLKQYFGGHFTLKMEGMVNDVTTARDNQANFEEYMRKQQESNHRVDLTVTVLTTGNWPSYKTSNINLPSEMIKCVEMFKDYYNSKQKSRRLTWIYSMGNCNIVAKFDAKPIELIVTTYQAAMLLLFNGSERLSYSEIVTQLNMPDDDAVRLLHSLSCAKYKILNKVPSNQTISPKDVFEINHKFTDKMRRIKVPLPPTDEKKKVVEDVNKDRRFSIDASIVRIMKSRKVMVHQQLVAECVEQLSRMFRPDIKIIKRRIEDLISREYLERDLETANTYRYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRZ9</accession>
    <name>A0A8I6XRZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123403336</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953212.1">
        <property type="nucleotide sequence ID" value="XM_045097277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRZ9"/>
    <dbReference type="SMR" id="A0A8I6XRZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558810"/>
    </dbReference>
    <dbReference type="GeneID" id="123403336"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0558810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403336"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="16310" checksum="FB715DBF4BDBAE84" modified="2022-05-25" version="1">MDASATVAAGKGKKGAAGRKAGGPRKKSVSRSVKAGLQFPVSRIGRFLKKGRYAQRVGSGAPVYLAAVLEYLAAELLELAGNAAKDNKKSRITPRHLLLAIRNDEELGKLLTGITIAHGGVIPNINPVLLPKKTAEKSPKEPKSPKKTAKSPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTP4</accession>
    <name>A0A8I6XTP4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123445419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978342.1">
        <property type="nucleotide sequence ID" value="XM_045122407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTP4"/>
    <dbReference type="SMR" id="A0A8I6XTP4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317520"/>
    </dbReference>
    <dbReference type="GeneID" id="123445419"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0265070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0265070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0265070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0317520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445419"/>
    <dbReference type="OMA" id="VFYNPVM"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000243">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF9">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="45483" checksum="4BF8E06793D477C8" modified="2022-05-25" version="1">MASASAAAQAVSFPPGRRAPLPPPRCTHSERGVSFDPGSAFYRSDSAPGRDLAVLAATLHRRRRPDPSAPFLCLDAMCGSGVRALRYLAQAGADFVWANDASDALHPVIVGNLSRFEPVPPDGQRRWVVSHLDATRLLAERYLRREYFDVIDVDSFGSEAEYIRAAFLALKIGGLMYLTSTDWRSARGYGGKCSLSSYGAYVRPVPYPNEIGLRMLLGGAAREAAMLGFHIKPVFSYYAYHGPIFRAMVQLCHGKEDGISNYGFICHCKSCGQSQTFGFDELGQISCRCADRTDATSITVVGPLWTGPLHDRSSITEMLSLAVEWGWAHTSVNGVTLEKLLGTMIEESDPRLPPGYIRLDEIASRAKVNSPPLGTLIHSLQKEGYAACRSHIGANAVKTNCPISSCIVVAREIRNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUT9</accession>
    <name>A0A8I6XUT9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">F-ATPase protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUT9"/>
    <dbReference type="SMR" id="A0A8I6XUT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0272660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0272660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0272660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0225930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0225930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0225930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0272660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0272660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0272660"/>
    </dbReference>
    <dbReference type="OMA" id="QSAYLFF"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00310">
        <property type="entry name" value="ATP-synt_Fo_a_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.220:FF:000003">
        <property type="entry name" value="ATP synthase subunit a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="entry name" value="ATP synthase, F0 complex, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="F1F0 ATP synthase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0138">CF(0)</keyword>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035150003" description="F-ATPase protein 6" evidence="16">
        <location>
            <begin position="22"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0272660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="25644" checksum="707B50D72C72DAFB" modified="2022-05-25" version="1">MLLTVVLVVFLFFLVTEKGGGKSVSNAWQSLVELIYDFMLNLVNEQIGGLSENVKQNIFPRISVTFTFSLFHNPHGMIPFSFTVTSHFLITLALPFSIFIGITIIGFQRHELHFFSFLLPAGVPLPLAPFLVLLELISYCFRALSLGIHLFANMMAGHSLVKILSGFAWTMLFLNNIFYFIGDLGPLFIVLALTGLELGVAISQSHVSTISICIYLNDATNLHQNESFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XV12</accession>
    <name>A0A8I6XV12_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123447426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979960.1">
        <property type="nucleotide sequence ID" value="XM_045124025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XV12"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413440"/>
    </dbReference>
    <dbReference type="GeneID" id="123447426"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344530.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447426"/>
    <dbReference type="OMA" id="MLSWGRS"/>
    <dbReference type="OrthoDB" id="1915958at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045320">
        <property type="entry name" value="JAGGED/SL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730:SF135">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730">
        <property type="entry name" value="ZINC FINGER PROTEIN JAGGED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413440.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="17420" checksum="F0E087C0A1FD7C3C" modified="2022-05-25" version="1">MDLSLTFGRSGTEGSGAAGRGASNGGLADYEARPLFPCLFCDRKFLKPQALGGHQNAHREERAAGWNPYLYGRRPDYSAASSRAAATNNFIPIATHGGGGGAAPPPLHVVPDAGPLTPSLHGVRAAAGPLSRVHEDVRSMLSWGRSPAAEPAPESNTGVEIDLELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWA2</accession>
    <name>A0A8I6XWA2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123401995</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044951785.1">
        <property type="nucleotide sequence ID" value="XM_045095850.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6XWA2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0539760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0539760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0539760"/>
    </dbReference>
    <dbReference type="GeneID" id="123401995"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0448800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0448800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0448800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0539760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0539760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0539760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401995"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000048">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000689">
        <property type="entry name" value="HECT ubiquitin protein ligase family protein KAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000007">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF4">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1451"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="609"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="919"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1073"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="923"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="941"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1081"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0539760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1843" mass="198734" checksum="468F23CAC17132B3" modified="2022-05-25" version="1">METRSRKRAAAASTPSSSSPSPSSSKRARTAPPPPLRARRAMDPSPSSRRRARAAAAAAEKGKDPDPSSTSNPAPPHHHHHEDEDDEGDDDGSAAPFPPSFTSASTALQGLLRRIGAGLDDLLPGSAAAAAASSTASAQLKRILAGLQAEGDESRQLVSLNQLCEMLCIGTEDSLAAFPVDAFVPLLVGLLREDDGPGASPNVMLLAARALANLVDVLPSACSSVVHYGAIPCFCARLLTIEYMDLAEQSLQALRKISLEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANICRKLPSDASEFVMEAVPLLTNLLHHHDSKVLEHASVCLTRIAEAFAHQPEKLDELCNHGLVAQAANLVSISNSPGQTSLSTSTYTGLIRLLSTCASGSLLATKTLLLLGISGTIKEILSGSGLVAGTSVAPALSRPADQMFEIVSLADDLLPHLPVGIISLPTNCRVFIKGSSTRRPAPTRQDGAGSTENERSGHESLLQEHPELLQQFGMDLLPVMTQVYGSSVNAPTRHKCLSIIGKLMCYSSAEMIQTLLSTTNISSFLAGILAWKDPQVLIPALQIAEIMMEKLPETFSKLFVREGVVHAVEALICPESSNMVPPQVPPQDKDGDSVMSSRPRRQRRRGGAAPTENSLLDVSNVSNSGIASAPPCSTEVPVTSLRFEVSDRAKSFKDKYFPSDHGSSDTGVTDDLLTLRALCAKLNTATDNVVTKAKGKSKALSASHFDISHDVEEQLDLIVNEMLAELSKANGVSTFEFIRSGVITSFLDYLSCGTFGKEKVSEANLPKLRQQALRRYKSFISVALSVDHERDETPMTLLVQKLQSALCSLERFPVMLSQSNRIGTGGSRLTSGLGALAQPFKLRLCRAQGEKSLRDYSSNIVLIDPFASLAAVEDFLWPRVQRGEAASKPQAASANNSESGTPGAAVGASSTPASGGRPTTRSKSSAASAGTSNKESLEESTCSAKAKGKAVVKPSSAEPKGPNTRNATRRKSASEKDLDMKQTHGDSSSEDEELDTSHIELDDALMIDDDDISEDEDDDHEVLQEGSLPICVQDGVHDVKLGDADDSNVGSASDNQAQPSSGSGSSARNIMGRGVNAAEFRSAGAFGSQGAMSFVAATMAGLTSGGGRGVRGGRDRRGLSLGGSMSEHNKLIFMAGGKQLSKNLTVYQAIQRQQMLDEDDEERFNGSDLPNDGNRFWGDVFTITYQKADSQAEKGSQGSSASLHQKSESCRSVSEAHTVSLLDSILQGELPCDLEKTNSTYNILALLRVLEGLNQLSPRLRAQTASDDFAEGKIATLDELYEAGTKVPSEEFVNSKLTPKLTRQMQDVLALCSGSLPSWCNQITKACPFLFPFETRRQYFHSTAFGLSRALNRLQQQQGDNPNTGSEREVRFGRLQRQKVRVSRNRILDSAAKVMEMFSSQRAVLEVEYFGEVGTGLGPTLEFYTLLGHELQSARLGLWRSSSPYDYSEMEIDKNGVIHADSDDDLPAPQELNSSEDARNLIQAPLGLFPRPWPSNVDTSEGSRFLKVVEYFRLVGRVVAKVLQDGRLLDLPLSTAFYKLILGQELDLFDIISFDAELGKTLQELQVLVERKRFFESSCGKDQLKVADLRFRGAPIEDLCLDFTLPGFPDYVLKEGEQNTIVNIHNLEEYVSLVVDATLKSGIMKQVEAFRSGFSQVFDISSLQIFSPQELDYLICGRQEIWEAESLVDNIKFDHGFTAKSPAIINLLEIMAEFTPDQQHAFCQFVTGASRLPTGGLAALSPRLTIVRKHPSSGVSALNTSGVTDAADDDLPSVMTCANYLKLPPYSTKEVMRKKLLYAILEGRGSFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYJ9</accession>
    <name>A0A8I6XYJ9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6XYJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0479370.1">
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="229"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0479370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="241" mass="25238" checksum="1F6F75015D9B5B69" modified="2022-05-25" version="1">MLSSQMLWEAIVKARKEANEVRKMARNKDAVAEERRVAAEERKVGMEERAKLLEWEKHLFFLDTSLFNDVQKEYVNLTHEEVLIQKRAMIRDMGGGGLGAMGGGGLGAMGGGGLGTMGGMGGFGAMGCMGAPPAAMGGMGGFGAPSDAMAAMGGMSFASLMGGMGAPPAAMGGMGAPPAMGERSFDVPPHTHSHEDAVEDLANTVGASRDAVRDEVREEDSSTEAKESSSEDDDEDEEDDD</sequence>
</entry>
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            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <lineage>
            <taxon>Eukaryota</taxon>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="210" mass="23733" checksum="86C5DDDDF1B3E22F" modified="2022-05-25" version="1">MTVTLQDIAMITGLLIEGRGLIGKVRSDGWRQRLAGLVGVEPKPWTHETRKDPRPSAVLFSWIQRHFRKCPKDGSTDIVERYARAYLCNLLTQVVFRDGTGDTTSWMFLDPLHDWDVKWSWGSAALAFLYHQLDGACMRNKPTSTLGGFVSALQVCMWEPILVGRNLSLPPNEPWKYPFGGDEEQYPTIAHTWANVQWTSTAAMGGTRHT</sequence>
</entry>
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        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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            <end position="21"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
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    <reference evidence="6" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2G0"/>
    <dbReference type="SMR" id="A0A8I6Y2G0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
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        <property type="entry name" value="OS11G0619800 PROTEIN"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
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        <location>
            <begin position="82"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15646" checksum="4C4CEB97A17CB0A0" modified="2022-05-25" version="1">MTELFGPLKENTDASVRIADMEAKVFKAMLHFIYTDSQLPMDDEQEVVVMAQHVLVAIDTYNLERLKLVCVEKLCNHMDASTAATTLALAEQHSCCGLKDLCFKFLASPGNMLKAVVANDGFEHVRSSCPSVLKELVAKLSP</sequence>
</entry>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2L1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592360.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="243" mass="26941" checksum="35A524D3B05F60EC" modified="2022-05-25" version="1">MSTTGSIQPSRPDIRSSPSASSENVRARNSGSSSRSAASPPAAPQAGAANPLRFDARTIHFSVNAWVLVVAGVGMLPMLPKHLADRACKLSLLGTVLSSGYSLYSTYGKPRELNMPAIQSWLQSVLGAKDFIHLMFSLLLVTSQLHLKIAALPVFCWALYHVARFLRRNFSRSSFYRGYLEEPCLWVDTNNTTLSLLSSNAELPLGFLLIISLFSWQRNFMQTFMYSNVLKVMYRAPVTSSYH</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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            <end position="160"/>
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            <end position="139"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0422930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="179" mass="20034" checksum="48BB974AA356C2C5" modified="2022-05-25" version="1">MEPPIHPHVLVSRLFSHQLHAINHHHTSMHLLLRRQPTSLHDGTQAHVPTEPRSARTRRGSGLDAVLRRHHGANQRHSSGNRRQRSRPRCAGGFNSRLASSRCGDGASHGHGVQEDPVGATGRVRAGGRGGVSAAGAGAGDTSQRRRSRLRQQRVQRWPGRLRRMLRRRVRVQRRSRVQ</sequence>
</entry>
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    <accession>A0A8I6Y543</accession>
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        <recommendedName>
            <fullName evidence="6">Sn1-specific diacylglycerol lipase alpha</fullName>
        </recommendedName>
    </protein>
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        <lineage>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044955615.1">
        <property type="nucleotide sequence ID" value="XM_045099680.1"/>
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    <dbReference type="SMR" id="A0A8I6Y543"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620760.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123406205"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0514940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0514940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406205"/>
    <dbReference type="OMA" id="MPIYRRQ"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023">
        <property type="entry name" value="LIPASE CLASS 3 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023:SF5">
        <property type="entry name" value="OS02G0780700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="3">
        <location>
            <begin position="57"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="191"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="72126" checksum="C67B462347BB5B0E" modified="2022-05-25" version="1">MATATMATAAGAAALLYYTLNRRLHTEKLDREEDSDSENGRDVASSGALESHSMSRVSRRDVRAPATWLETISTLSETLRFTYSETLGKWPIGDLAFGISFLLKRQGNLSVASIYAGDDSVELKGASVVTDLKYLLNLLTLCWHFSKKPFPLFLEATGYSTEDVIMQEPKAGILKPAFTILLDRDRQCILLLIRGTHSIRDTLTAATGAVVPFHHTIVKEGGVSDLVLGYAHFGMVAAARWIAKLATPCLTEALRLYPDYKIKVVGHSLGGGTAALLTYVLREQQEFATATCVSFAPAACMTWELAESGVHFITTVINGADLVPTFSAAAVDDLRSEVTASAWLNDLRHQIEQTRILSTFYRSASALGSRLPSMANAKARVAGAGAILRPVSNGTQVVMRRARSVAQAAWTRPGLQLSSWACIGPRRRNTTSIVTSEEITTSTTNGGSESTSLLTETTMETTEIVTSETTQYAASEVQSTISASDAVSTLDEKVDSDGEDIIDHQVDEDRITEVELWQQLENELYRKSEGENDIVEEMTESNIAEEVGGRAQDVLGEPNEKEVHRFYPPGKIMHIITSTREATIDDEETSVHEDEEPDLHQVDATGESETSIGIFLTPRSLYGKLRLSKMMINDHYMPMYRRNIEQLVAELEKDLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7Q7</accession>
    <name>A0A8I6Y7Q7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7Q7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0531950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0531950.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0531950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0640210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33800:SF30">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33800">
        <property type="entry name" value="OS06G0113600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="29"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="157" mass="18029" checksum="8F271904D15F743D" modified="2022-05-25" version="1">MDSGGMLFVVHLAPRIRVQMMAVRLGEISMRHLSNIYLVACGDMLLLVGCWGLFPTRGDSFEAFRLDQSTEHANLVKVEKLENWAFFISTDKRSQPLSFMNPERWGGRRNCVYYYRHDSEHWAAFELGKPASSRNSFVFKSSSNVVQPMWVVPSMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7T3</accession>
    <name>A0A8I6Y7T3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123446604</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979112.1">
        <property type="nucleotide sequence ID" value="XM_045123177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7T3"/>
    <dbReference type="SMR" id="A0A8I6Y7T3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0385430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0385430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0385430"/>
    </dbReference>
    <dbReference type="GeneID" id="123446604"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0320630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0320630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0320630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0385430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0385430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0385430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446604"/>
    <dbReference type="OMA" id="MSCFLED"/>
    <dbReference type="OrthoDB" id="778083at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF170">
        <property type="entry name" value="DOMAIN PROTEIN 1, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="51"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0385430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="224" mass="24127" checksum="0CB4CE29206777A8" modified="2022-05-25" version="1">MDYSNEATNTAAAQPYGRSMSPPSRVSSCSPPPVFPLMGNAPSSPPTIVLSPCAACKVLRRRCADGCMLAPYFPPTEPAKFTTAHRVFGASNIIKLLQDLPESSRADAVSSMVYEAEARLRDPVYGCAGAVCRLQKEANELKVDLARAQADLLSIQTQHANLLALVCVEFAANHRGDQQHQHQPPPLADQLNSIGGSGGGALYQQLYDSDLDSASWEEARQLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y897</accession>
    <name>A0A8I6Y897_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibits both subtilisin and chymotrypsin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y897"/>
    <dbReference type="SMR" id="A0A8I6Y897"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0457890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0457890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0457890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0551050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551050.1.CDS1"/>
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        <location>
            <begin position="29"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00002177"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="73" mass="8103" checksum="6275F28DE193B9FD" modified="2022-05-25" version="1">MSTSGKSSWPELLGWPVRPAMLTVKNERPEVSIEQLPEGTTPTPPGFRSDRVVVFYHTQDELGRRVATIPVIG</sequence>
</entry>
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    <accession>A0A8I6Y8B4</accession>
    <name>A0A8I6Y8B4_HORVV</name>
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            <fullName evidence="2">DUF3615 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC123405843</name>
    </gene>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="278" mass="30855" checksum="FC184724269D4C96" modified="2022-05-25" version="1">MNGLHDEPRRPKRRPERSAPEAKVAEPPARRAKRAEGHLPLRKRESPRFAQPADSEVAVASPLPPPPARGAETPAPPPKRRAAPPSSPHRDSEDVKAMAPPPKPEYSVPPNWHRTAYRLRNRVVRDTSNRKPAPSRYDNGIEIKHDPTWQTPACARVALEHYNNMNQEDEHEMVKAVSSHVFVFNGIWFHANFLAKRKGATNCVDLVPKYFFAESEIVGTRKLSCVSCVKLDPDDPKNIGGCSLCSEHILHPAGGGYHGAQAQSVRHAPGGLQISFKF</sequence>
</entry>
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    <accession>A0A8I6Y9N0</accession>
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        <recommendedName>
            <fullName evidence="2">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
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    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9N0"/>
    <dbReference type="SMR" id="A0A8I6Y9N0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547020.1.CDS1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="73"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="227" mass="25161" checksum="2C3BE557287C2832" modified="2022-05-25" version="1">MHDIVRSFAQFVARDEALAARTGETSIGSKLTAQKFLRLSLDNEASESDGLKWTSLQTQSTLRTLISVGHINMKHGDSLVHFPCLRTLHIDSGHVGLLVESLNEFNNLRYLSLQNSDISSLPDSIGKMKFLQCINLVGCKQFVKLPCSILKLGQLRYLNFYDTSINSMPRGFCVLTNLRSLAGFPALVDGDWCSLEEFGPLSWLKNLGLDRLENVIASSSAAKANWP</sequence>
</entry>
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    <accession>A0A8I6YBA9</accession>
    <name>A0A8I6YBA9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123413063</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044961945.1">
        <property type="nucleotide sequence ID" value="XM_045106010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBA9"/>
    <dbReference type="SMR" id="A0A8I6YBA9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651600"/>
    </dbReference>
    <dbReference type="GeneID" id="123413063"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540830.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0651600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413063"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAE" evidence="5">
        <location>
            <begin position="27"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="6">
        <location>
            <begin position="332"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="461" mass="51136" checksum="5511E7515649E9B3" modified="2022-05-25" version="1">MELLAVPVLLAAMAMTLTYLAWTAASRRRRSQCYLLDYVCYKPPDDRKLTTDMGFAVAERNKQLGAPELRYLLRLTSRAGLGEQTYLPSGALAGREKCLTYQDAHDEMDAFFIDAVAGLFAKTVFGPRDVDVLVVNVNIFCPQPCLASRIAHHYGMRDDVVAYNVSGMGCSATLVSLDIVQNVMRARSPCPVLALVVSTEVLSTGWYGGRDRSMMLGLCLFRCGGAAALLTSDPALGGRAKMKLRRLVRATVAANDDAYSAIFQREDADNITGFSINKTLPKAAVQAFAANLKRLVPFILPARELVRLAASFTWQKMQRRQRVKINVNLKTGVDHFCLHSGGVAVIDAVKKNFGLNEADVEPSRMTLHRWGNTSASSVWYVLAYMEAKRRLKRRDRVLMVTFGSGFKCNSCMWEVTRDLADKGAWADCIDEYPVESTANPYTDKYSWMNDNADDDSTLPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBK8</accession>
    <name>A0A8I6YBK8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="3 14">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 14">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123450035</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="14">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="3 14"/>
    <dbReference type="RefSeq" id="XP_044983368.1">
        <property type="nucleotide sequence ID" value="XM_045127433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBK8"/>
    <dbReference type="SMR" id="A0A8I6YBK8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0417390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0417390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0417390"/>
    </dbReference>
    <dbReference type="GeneID" id="123450035"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0347600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0347600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0347600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0417390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0417390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0417390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450035"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046901">
        <property type="term" value="P:tetrahydrofolylpolyglutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000008">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.20:FF:000011">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF16">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0417390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="586" mass="64827" checksum="0744FA51D5D46A0E" modified="2022-05-25" version="1">MQQRCGAHHGHGHGHGGTTTTPTYPTLAPTTTARITRGRAPPMPHPPLAAAATLRRRLLPLSSPLSSFSSSRVRLLLPRAMKSSSTATRHAGAAAEGEYEEVLGRLSSLITQKVRAHTGNRGNQWDLMHRYLQILELEEPIARMKVIHVAGTKGKGSTCTFTESILRSCGFRTGLFTSPHLMDVRERFRLDGVDISEEKFLNYFWSCWNKLKEKTDDDIPMPPYFRFMALLAFKIFSAEQVDVALLEVGLGGKFDATNVVKTPVVCGISSLGYDHMEILGNTLGEIAGEKAGIFKKGVQAYTAPQPEEAMIALRQRASELGISLQVADPLKPHQLKDQYLGLHGEHQYVNAGLAVALASTWLEKQGHMDRMPLNRTDPLPDQFIRGLSSASLQGRAQIVSDSQVNSEEKDRDSSLVFYLDGAHSPESMEICARWFSHATKEQSQTCSKSRRILLFNCMSVRDPMRLLPHLVDTATQNGAHFDLALFVPNQSQYNKLGSKASAPAEPEQIDLSWQLSLQAVWEKLLHDDNKGSNSTDSGETSLVFESLPLAIEWLRKNTVEDPSTSYQVLVTGSLHLIGDVLRLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YED9</accession>
    <name>A0A8I6YED9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YED9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0622160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0622160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0622160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0622160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0622160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0622160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF189">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="6">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0622160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="195" mass="22895" checksum="FA9E6F688390B486" modified="2022-05-25" version="1">MPPRRRSASGYRGVRQCPNGGFYAEIRSDDLRLSLGTYDTAHEAARAFDAAAWRLGRPRLQMNFPDVHTLQQALDLAPPPRLNSAEDRAEHTALQRCLLVAQEDERVMAEWRRRHPEDVAYEQEYWERRRKEDTRRRRERLDRRRRKALACAQADLVNAGGRSFFTEEDERWFDIWLSTSDDTNDDDDGADDWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFT9</accession>
    <name>A0A8I6YFT9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123404199</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044954050.1">
        <property type="nucleotide sequence ID" value="XM_045098115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFT9"/>
    <dbReference type="SMR" id="A0A8I6YFT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620410"/>
    </dbReference>
    <dbReference type="GeneID" id="123404199"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0514630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0514630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0514630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404199"/>
    <dbReference type="OMA" id="RFDIDIM"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008264">
        <property type="entry name" value="Beta_glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00737">
        <property type="entry name" value="GLHYDRLASE16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338831" description="Xyloglucan endotransglucosylase/hydrolase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="297" mass="33764" checksum="C53926BA18A75B39" modified="2022-05-25" version="1">MAPSLPSSSSCWHSALLVAMLVLVVVMDQVAMAYLDDDIEVVWGDDHSFFYMDDAGDDEILALCLDETHGSGFHTKEAYLYARFDVDLMLVPDNSAGTVTTLYLMPEDVPWDYHDEVDLEFLGNVTGEPYTLHTNIFANGVGNREEQFRLWFDPTADFHTYSIDWNPKRITILVDGVPIRSFRNNEEHGVAFPTWQKMRLHGSLWNADDWATQGGRVKTDWSGAPFFARYRNLRASWCRPSPGVAWCGDEPPGSTWFERGLDAAALRRARDAHMIYDYCKDLQRYKGSGLPKECVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YH13</accession>
    <name>A0A8I6YH13_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123409120</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <source>
            <strain evidence="10">subsp. vulgare</strain>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="421" mass="46155" checksum="6AE2286E8C656873" modified="2022-05-25" version="1">MDSSSLARKLRSLCIAGELSRAVSLLHRSAVRPVSSAYALLLQECVNRRDARLGKRIHARIVSTGFRCSDYMATKLLIFYAKIGELGVARGLFDGMPRRDVVAWNAMVSGCARGAEEAQAVEMFGLMRAEGLRPDQFTFASVLCACARMAALEHGRRVHGVMVKSDRVSGNLFANSALVDMYLKCSSAEDARRAFAAAPERNVTMWTALISGHGQHGHVREALALFDEMTRDGFRPNDVTFLAVLSACAHGGLVDEGLRHFSSMPSDYGLAPKGAHYAAVVDMLARVGRLHDAYEVVKNLPDCQEHSVVWGALLGACRKHGDVRLLELAVPRFFRLQPGNAGKYVVLANTYATCQMWDSVAGMHEAMKSLGIRKDPAWSAVEVQGKKHIFLARDTYHDDCSEIYEACNALARAVSEHSVRV</sequence>
</entry>
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            <fullName evidence="4">PI-PLC X domain-containing protein</fullName>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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    <reference evidence="3" key="1">
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956656.1">
        <property type="nucleotide sequence ID" value="XM_045100721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHY8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707220"/>
    </dbReference>
    <dbReference type="GeneID" id="123407558"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0586520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0586520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0586520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0707220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407558"/>
    <dbReference type="OrthoDB" id="7984201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08588">
        <property type="entry name" value="PI-PLCc_At5g67130_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF145">
        <property type="entry name" value="OS08G0103500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035248689" description="PI-PLC X domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="42983" checksum="69DFCDD14F450CAE" modified="2022-05-25" version="1">MAAAGLRPLAVALLLLIVFVASAVDARVGDECSAGAGCGSGLHCAACGDGGAGVCARAAPVDPLTHGTGLPFNNYSWLTTHNSFALAGAVSATGAAIITPTNQEDSVTAQLKNGIRGLMLDTYDFNNDVWLCHSFGGKCYNFTAFQPAINVFKEIQTFLEANPSEVITVFLEDYTVAGSLPKVFNASGLMKYWFPVAKMPKTGGNWPLLKDMVSQNERLLVFTSKKAKEAGEGIAFEWNYVVESQYGNEGMVEGKCPNRAESPAMDSKSQSLVLMNFFTTDPNPTGVCGNNSAPLVSMLKTCHDLSGNRWPNYIAVDYYMRSNGGGAPLVSDVANGHLVCGCDNIAYCKANSTFGTCVIPPPPPPSPPRAPAKGASPGGDPNAAVARLPAFQWSCLLGLMSLALLFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIM3</accession>
    <name>A0A8I6YIM3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein S1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIM3"/>
    <dbReference type="SMR" id="A0A8I6YIM3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0576810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0576810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0576810"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0576810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0576810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0576810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0648110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648110"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13197" checksum="0B799234241F0587" modified="2022-05-25" version="1">MNHLSKSEQPPGLMILWTLDVAAGEPRLLPQRFRQNRAWIELKKIWRTKKNVKGFSIKKIKGGYSVAIAGFITFLPFKKALHKKRIANDRFTIDSIKPTRRDIVIIVTSGLPCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YL63</accession>
    <name>A0A8I6YL63_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123407216</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044956229.1">
        <property type="nucleotide sequence ID" value="XM_045100294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YL63"/>
    <dbReference type="SMR" id="A0A8I6YL63"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726150"/>
    </dbReference>
    <dbReference type="GeneID" id="123407216"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0602230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0602230.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0602230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407216"/>
    <dbReference type="OrthoDB" id="2151624at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF86">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035150013" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="11">
        <location>
            <begin position="29"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="266" mass="28298" checksum="C7512F79243DDB2B" modified="2022-05-25" version="1">MSDGMKKSLLSLMLLLSCCSTPCSGSGDEPDVRCLKSFLRSVVDPGGALTSSWNFDNTTKGYICGFTGVECWSADQPKVWSLRLSGLGLQGPFPRGLRDCASMTGALDLSGNSFAGPVPADISLQLPFVTSLDLSYNGFRGAIPPGMGKMEFLTHVDLGHNQLSGQIPLELFRLPFLASMNVADNSLSGPIPPFLQRFPASSFAGNDGLCGAPLDLCPASASREQISAGSSVGAAVGFVLGFVVAFYFPQWFVFSGRLHPYVFRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YM30</accession>
    <name>A0A8I6YM30_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M3A/M3B catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123410753</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959630.1">
        <property type="nucleotide sequence ID" value="XM_045103695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YM30"/>
    <dbReference type="SMR" id="A0A8I6YM30"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731690"/>
    </dbReference>
    <dbReference type="GeneID" id="123410753"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0607130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0607130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0607130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0731690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410753"/>
    <dbReference type="OrthoDB" id="17530at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000019">
        <property type="entry name" value="Mitochondrial intermediate peptidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="11">
        <location>
            <begin position="247"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="699" mass="78406" checksum="332CF74B6B84923E" modified="2022-05-25" version="1">MLRAAAAARRAAVAAFSASAGRPETGLYGFEVLRTAKGFRRFVDEAIQRSDELVAHIAQLPPAAEIVRTMDEISNTVCSVIDSAELCRNTHPDREFAEEADKASMRIYEHLQYLNTNTTLYNAIVKAESEGALLSEESRKAANNLRADFEKGGIHLPKDKLERVNQLNLAIAHLGRKFNENVMNKPGFVDIYPASRIPRNIQYSFKPISRFKPRGVEEQSNAMNTTRQKGVRIVTDSGSVSSALRWVKDEEVRRQVYVVGNSEPRENIGVLDELIDARDEFAKTMGCRSYAEFAIRPNMAASVDVVMSFLNDLSDTVRHKADEEFNTIKDFKRRVCNDKSADLEPWDEDYFIGMMKSSAHTVDPSVVASYFPLSQCIKGLNVLVESLFGATFHEVPMGDGESWHPNVIKLSLHHPDEGDLGFMYLDLYSRKGKYPGCAHFAIRGGRRLSDTNYQLPIIALVCNFSSSRGLTARLNHCDVETLFHEFGHALHSLLSRTEYQHFSGTRVALDVAETPSNLFEFYAWDYRVLRTFALDETTGDPIPEKLVKALNASRNMFPATDLQRQVFYSIMDLTLFGEHTSKPVDTISAVADLKRKHTSWNYVEGTHWHTRFSHLINYGAGYYSYLYARCFATTIWQEVCQGDPLSRNTGSAIRDKFLRHGGAKDPSALLKDFVGDSVIKNSGGGIIPDISSLCKEVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YTW7</accession>
    <name>A0A8I6YTW7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123397803</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044948269.1">
        <property type="nucleotide sequence ID" value="XM_045092334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YTW7"/>
    <dbReference type="SMR" id="A0A8I6YTW7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497430"/>
    </dbReference>
    <dbReference type="GeneID" id="123397803"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0412610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0412610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0412610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397803"/>
    <dbReference type="OMA" id="GHVEAPK"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="6">
        <location>
            <begin position="58"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="7">
        <location>
            <begin position="374"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="486" mass="52521" checksum="5B1AFEF5E1EBEEA8" modified="2022-05-25" version="1">MEMAHRDHLLAAVHRVLGVAVLILCLVAELLVLVFRRHAALYLLPVCAMMTHLWRSGRRAGIGLVDFAYLKPPRRLRVTIPGLLEHLRLIGCFDDGSVEFMSRVVEDSGMGDETYFPPSLHYLPPSAAHADAVQEAGMLFFPTLDELFAKTGVPPSAVGALVVNCSGFGPAPSLTAIIANRYRMPSDVKTFNLSGMGCAAGIVGVDVARGVLKAHAGAIDYAVVVSAEIVTIGWYRGRDPTKLLLNCFFRTGCAAALLSNTVSVPAPVKYRLVALKRTTIAADDRGYNLAVREEDDEGITGFTIGRGLGRVFRDLLRAHLPAFGASILPWHEKIRYAAALMRFHRRRRSSRKLQGGAGHVEAPKPNFLAAASHFCLPSSGMPNIRRLAEGLGLGEREAEAALATFQRFGNQSASSLWYQLGYHEARGRVRRGDRVWQLGMGTGPKASSALWERVAADAGAAAADEGPWGDCVLRYPARAPCGAAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YUM3</accession>
    <name>A0A8I6YUM3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0003310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="61073" checksum="6660DE6BCACB9F2F" modified="2022-05-25" version="1">MAATSTSASSPAAADDDLHAFLRGGLRTRGAVLRAHAFLLRRGLLLGHPVPSGLLLSAAAATTTATHLLRLLLRHLPPPLPLFSLSAALRAAAPRVPFSALLSLFAHLLRAHPASGFPDAFAFPPLLSAAASARSPRRHLPAALALHAQLLRRGLLFALPPHAANALLHFYAAAGSLPSARHLFDEMPSRDIASHNTMMTAHAAALGGGVDAARQLFDGMLLRNVVSWNVMINGYVKAKRPQQALEVVRWMAGVGVRGTATTMVGAATACARLGRLGPGREVHCAFLRRFEEDNLLFWTSLVDMYGKCRRVAAARKVFDRLNVRNVVCWNAMIIGHCVYGEPSDGLQLFQEMIGRGKNGSDDQWVLGPDEVTFIGVLCACTRLGLLDAGKAYFNQMTTTYSLRPTFAHYWCMANLYGSVGLLEEAEGLLRSVPEELKARALGGLLGLCRFRGEWKLGERIALRLIELEPSNCAHYALLCSVYASAGRWEDAHRVKAIIKESDERFSPGHRLVDLNEIANEFKIRERQPENQEIYVILDDLVSKLQFTSREDAHTEPGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0V3</accession>
    <name>A0A8I7B0V3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7597 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0V3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0042190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0042190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0042190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0032740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0032740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0032740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0042190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0042190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0042190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056018">
        <property type="entry name" value="DUF7597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075:SF10">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075">
        <property type="entry name" value="OS02G0499800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24530">
        <property type="entry name" value="DUF7597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7597" evidence="2">
        <location>
            <begin position="7"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0042190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="486" mass="52467" checksum="6CAD03158B9856E0" modified="2022-05-25" version="1">MANFELDSEFFLPSGHNIIDGGPDRLPRTYTTPAVPITRRHECFVIADVHPAPPTDNLVQVRQEVAALLLHKRLHVRSAQPCIEGVGLFELRDAAESFPTVQMVPQEVAHNFVVCFLRHDQCVGFRGQEGFRQGCLMLLGVPLDFRNTEDLRAAVNTFGEFHHWVSDDPYLVSWSAPVYILGAVPAELLPNDEDPMPLNGNPHPLPGQLVNDNLLFALPPYPALGWNAVPPPPEEPVVPGADDDGGWGQPPAAAEGGGWEPEAAPEVPHADPAHDQESMVIDQPSPSSSASVHELVDLDPHLHGDIEAEAADHAPPAPDLDEIMAADPALTLIWHRLSSPLLLKLPPPEPASNTLSLEEVIPVSPSSSATDSFSFQSPAPSKKGSRKKATPVVDSSVRRCTRGSIKQDGFKPVLQELPVHVPKKRKPKAKPMPSPAQESAEVQVPPTTPIPVIQAVGQMLGIAPEKVTVDRLMEDPADPTPSSPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0Y3</accession>
    <name>A0A8I7B0Y3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123433177</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May only reduce GSH-thiol disulfides, but not protein disulfides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CGFS subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971372.1">
        <property type="nucleotide sequence ID" value="XM_045115437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0Y3"/>
    <dbReference type="SMR" id="A0A8I7B0Y3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0047590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0047590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0047590"/>
    </dbReference>
    <dbReference type="GeneID" id="123433177"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0037130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0037130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0037130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0047590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0047590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0047590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123433177"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000092">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0047590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="489" mass="52890" checksum="B9F9D10E0764D9BB" modified="2022-05-25" version="1">MASGGGAVREVGSRAELDAAVGGARAAAVHFWAAWCEASKQMDEVFAHLAVDFPHALFLRVEAEEQPEISEAYGVTAVPYFVFCKEGKPVDTLEGANPASLANKVAKLAGPANVAQSAVPASLGVAAGPAVLEKVQEMARQNGSSAAESTLKKRLEQLVNSHPVILFMKGNPGEPRCGFSRRVVDILKQEGVEFGSFDILSDNEVREGLKKFSNWPTFPQLYCKGELLGGCDIVIAMHESGELKDMLKEHNIPLGQQGTKIEEPVISESANEQSPEAIGLTEAQKARLESLTNSNPVMIFIKGSPEEPKCGFSGKVVHILKQEKIPFSSFDILTDDEVRQGLKVLSNWPSYPQVYIKGELVGGSDIVMEMHKSGELKKVLTEKGIIPEESLDGRLEALISSSPVMLFMKGNPDNPRCGFSSKVVNALKGAGISFGSFDILSDEEVRQGLKTYSNWPTFPQLYYKSELMGGCDIVLELEKSGELKATLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4Y0</accession>
    <name>A0A8I7B4Y0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4Y0"/>
    <dbReference type="SMR" id="A0A8I7B4Y0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0114890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF48">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="198" mass="22292" checksum="C834E992233C81F3" modified="2022-05-25" version="1">MERMAQSYFKEIYTKDPTLDPSGVLECIQQRVTTEMNDALGLPFSDKEISDALFQIGPLKAPGVDGFPARFYQRNWDVLKADIIAAIQEFFVTGIMPDGVNDTSVVLIPKIPHPRELKDFRPISLCKVVYKIVSKCMVNRLRPLLAELISENQSAFIPGRLITDNSIIAFECIHHIQSVKENSPALCAYKLDLSKAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B515</accession>
    <name>A0A8I7B515_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123443137</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="RefSeq" id="XP_044975348.1">
        <property type="nucleotide sequence ID" value="XM_045119413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B515"/>
    <dbReference type="SMR" id="A0A8I7B515"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245220"/>
    </dbReference>
    <dbReference type="GeneID" id="123443137"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0203550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0203550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0203550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0245220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443137"/>
    <dbReference type="OMA" id="PRIWNNT"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035340885" description="Peroxidase" evidence="18">
        <location>
            <begin position="24"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="34"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="119"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="352" mass="37268" checksum="2ACE49B08EBE774B" modified="2022-05-25" version="1">MVAAIVAVVMVAVCLRLPAVARAQLQVGFYNTSCPNAESLVRQAVASAFANDSGIAAGLIRLHFHDCFVRGCDASVLLVSANGTAERDAAPNKPSLRGFEVIDAAKAAVEKSCARTVSCADIVAFAARDSINLTGQAAYQVPSGRRDGNFSLDQDALNNLPPPTFTAKQLVDRFANKTLTAEEMVILSGAHTVGRSFCSSFLARIWNNTTATVDTGLSPGYAALLQALCPSSPNAATTTAVDPSTPAVLDNNYYKLLSLNLGLFFSDNQLRTNATLNASVNSFAANETLWKQKFVAAMVKMGNIEVLTSTQGEIRLNCSVVNNRSSVAAPEIQMSRHYYSGSTASIEEIATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7T8</accession>
    <name>A0A8I7B7T8_HORVV</name>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449957</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7T8"/>
    <dbReference type="SMR" id="A0A8I7B7T8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0415090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415090"/>
    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:photosynthesis, light reaction"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
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        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF1">
        <property type="entry name" value="VOLTAGE-DEPENDENT CHLORIDE CHANNEL 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bestrophin_2"/>
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    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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            <begin position="157"/>
            <end position="179"/>
        </location>
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            <begin position="185"/>
            <end position="207"/>
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        <location>
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        </location>
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        <location>
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            <end position="34"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="466" mass="51466" checksum="65A08DA5501D1F15" modified="2022-05-25" version="1">MDTTHVGQRVEARRAPHPLLSSATQTPLRSTAGNGLSRAPHPLPSPPRTDRGQAMAARPPPSPASTPRRHHHHLSPAARPGRRDRQLARCFPEPFPKPPNPPNPLLSLLTAVPDWADAVSERRIREPRPLYTHEQWREHRSSLRHLRHLLSSLSSRVILSLVPPVSAFTAFAAAVATYNTLAPDYALTASSLPYQLTAPALALLLVFRTEASYARFDEGRKAWMRVLAGATELAGMVMHSCAGAGAGERGDNDTGTGALVNYILAFPVALKCHITSNSDIRKDLEGLLAEDDLNVIMSSKHRPRCIIEFISQSLQILDFEEHKRSVMESKLSCFLEGICVCEQIIGIPVPLSYTRLTSRFLVLWHLTLPIILWAECKWIVVPATFVSAASLFCIEEVGVLIEEPFPMLALDAQCKQLHDSMRDMMSVQGLVRKQLVAKTKGRGRGGRLPQNGWPVSSPRSEQVKVD</sequence>
</entry>
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    <accession>A0A8I7B9W4</accession>
    <name>A0A8I7B9W4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC123452365</name>
    </gene>
    <organism evidence="3 4">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the QWRF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984951.1">
        <property type="nucleotide sequence ID" value="XM_045129016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9W4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0512660"/>
    </dbReference>
    <dbReference type="GeneID" id="123452365"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0425870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0425870"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807:SF43">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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            <begin position="1"/>
            <end position="11"/>
        </location>
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        <location>
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            <end position="41"/>
        </location>
    </feature>
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            <end position="94"/>
        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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            <end position="185"/>
        </location>
    </feature>
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        <location>
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            <end position="261"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="220" mass="25941" checksum="3B594CD6EFACECD2" modified="2022-05-25" version="1">MELVQMMKLLIRDIKVTKRKSRFWGVKINVFGRKEDPKTEDINYGTLTHDCDMGMNIVVHDAITRLSYRHRLELGTHYFGRLGWQQPEGAHIILTDEHEVKFTTVLVYNQELEHYIKNLQMDLLSKLFENDALRESLKTEKLEVAHVKKHDPEIQKLPQDLKNSQAMAHAGDVEIQKLKSQLLELQGRHRKLHVNKEYLVEELEKIKHDQKKPTDDEDMI</sequence>
</entry>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
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            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.3HG0232250"/>
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    <dbReference type="GO" id="GO:0016020">
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    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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</entry>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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</entry>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946433.1">
        <property type="nucleotide sequence ID" value="XM_045090498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BE07"/>
    <dbReference type="SMR" id="A0A8I7BE07"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0450670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0450670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0450670"/>
    </dbReference>
    <dbReference type="GeneID" id="123395495"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0450670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0450670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0450670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395495"/>
    <dbReference type="OMA" id="PCPGFQV"/>
    <dbReference type="OrthoDB" id="266663at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000053">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055183">
        <property type="entry name" value="PTEN2A/B_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF96">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND PROTEIN-TYROSINE-PHOSPHATASE PTEN2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22918">
        <property type="entry name" value="PTEN2_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="8">
        <location>
            <begin position="175"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="289"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="9">
        <location>
            <begin position="361"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="490"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0450670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="654" mass="71306" checksum="2147107B6781A4C5" modified="2022-05-25" version="1">MEQQQVKPSLPPSDTPMDNQDSSPPPAVTTADPVPRAPANDSATQVASPNPVAPSSPSAPTSATTQDAAGRETPSSMFSTSGLSSWAKNLKIPQPSSGQESPTGKNTVSRLASGLGLRLSPKDPQQDETAEGSTTPTTAQAGVFGSLTRGIVDSSKNAVKAVQVKARHMVSQNKRRYQEGGFDLDMTYITENIIAMGFPAGDLSSGIFGYFEGFYRNHMEEVIRFFEMHHKGKYKVYNLCSERLYDASLFEGKVACFPFDDHNCPPIQLVISFCQSAYSWLKEDIENVVVVHCKAGKARTGLMISSLLVYLKFFPTAEESIEYYNQKRCVDGKGLILPSQIRYVKYFERILTYFNGENQPPRRCMLRGFRLHRCPYWIRPSITVSNHNGVLFSTKKHPRTKELMPEDFWFTAPKKGIMVFALPGEPGLTEVAGDFKIQFHDRQGDFYCWLNTTMMENRVTLNPTDFDDFDKRKLPSPGFQVEIVLVDYSGSQPPKQKPADGSADKKCDADSSTSSNAKDSNAAPAESNKETGSDDKDDVFSDNEAEDGSSKGRKEDVSSSGQGGANRAKASETSVAQQEPSAAASGIEKITISGDQGIATAPDGISLKTEASSKSDSTKAQPPAMESSSLSEFKAIAADASVFSFGDEDDYESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BE90</accession>
    <name>A0A8I7BE90_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123452982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045129733.1"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0386480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0466870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0466870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0466870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452982"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16045">
        <property type="entry name" value="LisH_2"/>
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    </dbReference>
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        <property type="entry name" value="LISH"/>
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    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0466870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28986" checksum="A3E8B17CE159C9F5" modified="2022-05-25" version="1">MDDYAREMMELKTLVTRTLEKKGVLAKIRAELRASVFEAIEEEDRVIEENEDGGNPALLGSCNDRAKQLHASSSGRLLTALIGEYLEWAQLSHTMKVYLPECNLPKDFWKNELKDFSNKNGAEGSRSAESGPMLLDVLEGYLKYENLSQTRMAGRRMVNSESDPTLNTEHRNMRRPPSSSSVAGLPPMGRQMQPSQTSDRRGGSSASNTRKDEYNWGYDADDLSEEVLRTSTALENIQLDRKARNLTTSWRHPGNGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIH8</accession>
    <name>A0A8I7BIH8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123407474</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044956545.1">
        <property type="nucleotide sequence ID" value="XM_045100610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIH8"/>
    <dbReference type="SMR" id="A0A8I7BIH8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0660830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0660830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0660830"/>
    </dbReference>
    <dbReference type="GeneID" id="123407474"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0548450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0548450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0548450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0660830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0660830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0660830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407474"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042631">
        <property type="term" value="P:cellular response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902457">
        <property type="term" value="P:negative regulation of stomatal opening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000059">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="5"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0660830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="241" mass="27075" checksum="DE009BC71BD9122B" modified="2022-05-25" version="1">MADKGCLKRLQKEYHALCKEPVPQIVARPLPNDILEWHFVLEGSVATPFEGGYYYGKLKFPSDYPFKPPSISMTTPSGRFAPHKRICLSMSDFHPESWNPMWSVASILTGLLSFMMDDALTTGSIRSTDGEKKRLAKASLAYNCESKNCPHFRKLFPEYVEKYNQQQEKEQTGAEPEPQEKPAPSPSPADVQQAAAMVNNIGRPVAEVRGEKKLKKRVPFWLMVVIVSVFGAVMALPLMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJX2</accession>
    <name>A0A8I7BJX2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410940</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959799.1">
        <property type="nucleotide sequence ID" value="XM_045103864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJX2"/>
    <dbReference type="SMR" id="A0A8I7BJX2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0736000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0736000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0736000"/>
    </dbReference>
    <dbReference type="GeneID" id="123410940"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0610490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0610490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0610490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0736000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0736000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0736000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410940"/>
    <dbReference type="OMA" id="CHFNSDC"/>
    <dbReference type="OrthoDB" id="617225at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47849">
        <property type="entry name" value="CHITIN-BINDING LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47849:SF11">
        <property type="entry name" value="CHITIN-BINDING TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035199676" description="Chitin-binding type-1 domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="7">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="7">
        <location>
            <begin position="71"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="7">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="7">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0736000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="21882" checksum="DB2D9677260407DB" modified="2022-05-25" version="1">MKGLLLCTLALAFAAVTTHAQLQYCDKRCGKQADGMECPNNLCCSKDGYCGLGANYCSAAAGCQSGACYNNKMCGAQAGGALCPNNHCCSSSGRCGYGSEYCSGSRGCQSGPCWADLKCGHLANGKQCPNNLCCSRQGYCGLGPEFCGGACQNGACSTDKPCGKNANGARCTNNYCCSQYGFCGLGKDYCGTGCESGACYTPSFLADILKCLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2CR79</accession>
    <name>F2CR79_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123424383</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK354131">
        <property type="protein sequence ID" value="BAJ85350.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044963922.1">
        <property type="nucleotide sequence ID" value="XM_045107987.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044963928.1">
        <property type="nucleotide sequence ID" value="XM_045107993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CR79"/>
    <dbReference type="SMR" id="F2CR79"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0019100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0019100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0019100"/>
    </dbReference>
    <dbReference type="GeneID" id="123424383"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0014880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0014880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0014880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0019100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0019100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0019100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424383"/>
    <dbReference type="HOGENOM" id="CLU_033323_3_2_1"/>
    <dbReference type="OrthoDB" id="10261749at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ85350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0019100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="326" mass="37327" checksum="27DD75CBF67E9323" modified="2011-05-31" version="1">MSSSPRETAAASQGEGGKESCPFCEMSKQHAAGCARRLPKRIILVRHGESQGNLDMSAYSTTPDYRIPLTPRGVEQARAAGRGILDVVSSGAPDANWKVYFYVSPYERTRATLRGIGAAFPRHRVIGAREECRVREQDFGNFQVEERMRAIKETRERFGRFFFRFPEGESAADVFDRVASFLESLWRDIDNGRLDPSTTCETNLVIVSHGLTSRVFMMKWFKWTVEQFERLNNFENCEFRVMQLGPGGEYSLLMHHTKEELEAWGLSPEMITDQQWRASANRRSWSEECSSFIATFFDNWNDPPEGEEETDDDRQQEGDAKIESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2CRV5</accession>
    <name>F2CRV5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123406197</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK354357">
        <property type="protein sequence ID" value="BAJ85576.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955606.1">
        <property type="nucleotide sequence ID" value="XM_045099671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CRV5"/>
    <dbReference type="SMR" id="F2CRV5"/>
    <dbReference type="PaxDb" id="4513-MLOC_1509.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627470"/>
    </dbReference>
    <dbReference type="GeneID" id="123406197"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0520780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0520780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0520780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406197"/>
    <dbReference type="eggNOG" id="KOG2056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011840_0_0_1"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CRV5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF91">
        <property type="entry name" value="CLATHRIN INTERACTOR EPSIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="7">
        <location>
            <begin position="18"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="513"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="576"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="513"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="603"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="667"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ85576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="908" mass="96352" checksum="E32AC8D69734C6D2" modified="2011-05-31" version="1">MKKVFDQTVRDLKRGVNKKVLKVPGTEQKILDATSNEPWGPHGSLLAEIAQATHNYHEYQMIMNIVWKRVSDTGKNWRHVYKGLIVLDYLVAHGTERVIDDIREHSYQISALADFQYIDSSGRDQGSNVRRKSQSLVSLVNDKERILEVRQKALATRDKYRSAFATSGPHRSPGGYDNDRDRYEGGRYDNRNGYGRERDGYREDDRYSGAGDTPNRDGDRYSRDSNERNREDEYRGSNSNPEYAEGSGRRSYGDEEAYSSRGRGSNADAPTQDERPIERKPSNQQIASPPNYEDVTGDTQDNHHDERNGASVPAAAPKVSSPSVPRTSFPPVPGQVNGVHDKPVEVVAAQPPAPAEPNGFDEFDPRGSVPDASPPVNTSPIMNSFEMDLFGSDPIGALALVSVPQPTDVPNVEPPANSGFETDSFMGMPPASTGFSEAIDASNPFGDPTPFKAVQEENHAVSQTNATPAGSFQATGPGADVNPFQPASSTSFGFEDTLGDLSFASNAAPGQQDIFGSTTSLPTGVSHANPSQQAPSAYVPSQASQPVTHAPPQAVAPNTHAALMYSQPQAYPAALNPSSFPQAAAPSFAPPQAPQAAAPNLPSGPSNFFMQPASQNGAPPSYVPPQSSHLPPQHAPQQSFLPQTAAPAPQAPSISRGASQPFGAPNSVPSGANTPLQSSLSAPPETLISALQVSQTQPVKKFEPKSTVWSDTLTRGLVDFNISGAKTNPHADIGVDFDSINRKDKRQEKKISQAPVVSTITMGKAMGSGSGIGRAGASAVAPPSNPMGAGRGVGIGGAGYGGGMGMNRPMGMGMGMNQQPMGMGMGMNQQPMGMNQQPMGMGMGMNQQQMGMGMGMNQQQMGMNIGMGMNQGMGMRPPMGMAPGGMPGAGYNQMGAGYGGQQPYGGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2CY98</accession>
    <name>F2CY98_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402380</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356602">
        <property type="protein sequence ID" value="BAJ87819.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952230.1">
        <property type="nucleotide sequence ID" value="XM_045096295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CY98"/>
    <dbReference type="SMR" id="F2CY98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0578020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0578020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0578020"/>
    </dbReference>
    <dbReference type="GeneID" id="123402380"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0479270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0479270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0479270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0578020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0578020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0578020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402380"/>
    <dbReference type="HOGENOM" id="CLU_122141_1_0_1"/>
    <dbReference type="OrthoDB" id="1699369at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein At2g34160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF43">
        <property type="entry name" value="ALBA DNA_RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030333">
        <property type="entry name" value="UCP030333_Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ87819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0578020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="139" mass="15188" checksum="1849DD0F16ECEA68" modified="2011-05-31" version="1">MEEVTEAVNNLTIAEGAAATAGAEGHKKNRIQVSNTKKPLFFYVNLAKRYMQLHEEVELSALGMAIATVVTVAEILKNNGLAVEKKIMTSTVDVKDDTRNRPIQKAKIEILIGKTEKFDELMAAAAEEREAAGAEEEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZ26</accession>
    <name>F2CZ26_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123442814</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356882">
        <property type="protein sequence ID" value="BAJ88097.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359233">
        <property type="protein sequence ID" value="BAJ90444.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974897.1">
        <property type="nucleotide sequence ID" value="XM_045118962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZ26"/>
    <dbReference type="SMR" id="F2CZ26"/>
    <dbReference type="STRING" id="112509.F2CZ26"/>
    <dbReference type="PaxDb" id="4513-MLOC_80531.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234190"/>
    </dbReference>
    <dbReference type="GeneID" id="123442814"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0194350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0194350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0194350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0234190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442814"/>
    <dbReference type="eggNOG" id="ENOG502QVZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106897_0_0_1"/>
    <dbReference type="OMA" id="PIPHLLM"/>
    <dbReference type="OrthoDB" id="508390at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37910">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37910:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ88097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ90444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="23491" checksum="7E53D5754330109B" modified="2011-05-31" version="1">MQGPVTPARSVSTGETPLQRPPVIPSPPSASTRTRRQLLASAGGLFLVAAAGNNNNASRGAASAAGLDYDPVTEAERAASEAVSQRVGEAVRLLDAGRELQARGEFAGALASFTAVVSGYKDLALSEYARVGRAVVLYEIGDRDESITEMEDVSVALKGYPEIHAALAAALYADKHAPLLAEFQFNIATLLDPHYSDLAYVRDTKHWPPSLVASLKNFITLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F2D6M2</accession>
    <name>F2D6M2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123409139</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK359534">
        <property type="protein sequence ID" value="BAJ90743.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958054.1">
        <property type="nucleotide sequence ID" value="XM_045102119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D6M2"/>
    <dbReference type="SMR" id="F2D6M2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693440"/>
    </dbReference>
    <dbReference type="GeneID" id="123409139"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0693440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409139"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009773">
        <property type="term" value="P:photosynthetic electron transport in photosystem I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009644">
        <property type="term" value="P:response to high light intensity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037497">
        <property type="entry name" value="PGR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35709">
        <property type="entry name" value="PROTEIN PROTON GRADIENT REGULATION 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35709:SF1">
        <property type="entry name" value="PROTEIN PROTON GRADIENT REGULATION 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ90743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="121" mass="13058" checksum="0694F2FCD8D2B119" modified="2011-05-31" version="1">MTASVSYGLRALPTWSSSVSGDDHCSALAMSVSARPRSARPLRTPARMGNVNEGKGIFAPLVVVVRNIVGRKRFNQLRGKAIALHSQVITEFCKTIGADPKQRQGLIRLAKKNGEKLGFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2D9B1</accession>
    <name>F2D9B1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide methionine sulfoxide reductase A5</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123411016</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
            <tissue evidence="13">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRA family specifically reduces the MetSO S-enantiomer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="AK360473">
        <property type="protein sequence ID" value="BAJ91682.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK361645">
        <property type="protein sequence ID" value="BAJ92849.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371798">
        <property type="protein sequence ID" value="BAK02996.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959865.1">
        <property type="nucleotide sequence ID" value="XM_045103930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D9B1"/>
    <dbReference type="SMR" id="F2D9B1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0647630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647630"/>
    </dbReference>
    <dbReference type="GeneID" id="123411016"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0537520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0537520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0537520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0647630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0647630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411016"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF3">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008954677" description="Peptide methionine sulfoxide reductase A5" evidence="10">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="11">
        <location>
            <begin position="48"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ91682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK02996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="26791" checksum="8CB3BB7F7CD3BE62" modified="2011-05-31" version="1">MARASSSAAAAFLCALATLVAAASGARLAVRAGGTGLTVRAGGGASATAVFALGSFWRSEAAFGCLHGVLRTSVGYAGGSKPNPEYRNLADHAECVKVEYDPRQIQYKQLLDVFWASHDPREVFGQGPDVGNQYRSVIFTNGTLEARLAALMKEREQVKDRSSVITTKIQPLGAFYPAEPEHQKFELKRKPFLVQLIGNLPEEELLSSTLAAKLNAYAAELCPPKTQKRISSKIDEIAKKGWPILRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2DE42</accession>
    <name>F2DE42_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="27">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="28" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thaumatin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AK362159">
        <property type="protein sequence ID" value="BAJ93363.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371391">
        <property type="protein sequence ID" value="BAK02589.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DE42"/>
    <dbReference type="SMR" id="F2DE42"/>
    <dbReference type="PaxDb" id="4513-MLOC_62542.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0190270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0190270.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0190270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229420"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SK2Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_170_1_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DE42">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09217">
        <property type="entry name" value="TLP-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000003">
        <property type="entry name" value="Thaumatin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF9">
        <property type="entry name" value="OS01G0113650 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="15 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0295">Fungicide</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0611">Plant defense</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 25" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090703" description="non-specific serine/threonine protein kinase" evidence="25">
        <location>
            <begin position="29"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="328"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="BAJ93363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="641" mass="71145" checksum="3783AA5EF7B01630" modified="2011-05-31" version="1">MGTSSLSLHHPPPLLLLLRLLLLPFVTAQTILNITNRCPYTVWPAALPVGGGMRLDPGKTWILKVPYDTTGGRVWARTGCSFDGRGNGTCQTGDCQGMLACQTDGQPPYTSADFSLDQYVNYSFFDISLYQGFNVPMEFLPMPDKAQGGPGCTKGPHCGASITSQCPSELKAPGGCNSACTVFQKQNYDMYCCEEPPCESNKYSAFFVRMCPEAISYSSDAPTKTSFSCRFNTNYQVTFCPPLNLTSSPPSPPKSADLRTLRKRSSVGTSISVAIAGAIVSIVAFIFFIIRGRRTQRRQEMEEEEEEFGQLQGTPMRFTFQQLEAATEQFKDKLGEGGFGSVFEGQLGEERIAVKRLDRAGQGKREFLAEVQTIGSIHHINLVRLFGFCAEKSHRLLVYEYMSKGSLDKWIYARHENSAPPLEWRVRCKVITDIAKGLSYLHEDCMKRIAHLDVKPQNILLDDDFNAKLSDFGLCKLIDRDMSQVVTRMRGTPGYLAPEWLTSQITEKADVYSFGVVVMEIVSGRKNLDTSLSEESIHLITLLEEKVKSDHLEDLIDKSSNNMQADKRDAIQMMKLAMWCLQIDCKKRPKMSEVVKVLEGTMDADSNIDHNFVATNEANFGIAGNANSSAPPIATDLSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLT8</accession>
    <name>F2DLT8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123439442</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK364856">
        <property type="protein sequence ID" value="BAJ96059.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972088.1">
        <property type="nucleotide sequence ID" value="XM_045116153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DLT8"/>
    <dbReference type="SMR" id="F2DLT8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234240"/>
    </dbReference>
    <dbReference type="GeneID" id="123439442"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0194380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0194380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0194380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0234240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439442"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF14">
        <property type="entry name" value="OS01G0168800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="63"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ96059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="336" mass="35563" checksum="4B5E46BBE6091F04" modified="2011-05-31" version="1">MGFGVVSLLNAVFRRAFTSAGLRPSSVAVDAETTLHFWAHPSLLPSSSSDGDDKQRGRRPVAVLIHGFGPDPTWQWAAQVGPLSRHFDLVVPTLLFFGASTTRAPDRSDAFQAAAIAKLLGAVAVGGEEGRVVHVVGTSYGGLVAYHLARALQQQQGGAAGAGAGGWTVGKVAVCSSDLAKGAEDDRALAAKGGVADVTELMVPADTKALRRLMAICAHGPPKYLPECLARDLLRKCFAVQREGKIELIKGIASGHGFEITPLPQEVLIVWGEFDQIFPVAKAHKVKEKLGEKAKLRIIPNTGHLPHQEDSKLFNQILLDFLLPPPSSSNGIAAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQX9</accession>
    <name>F2DQX9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401985</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin-like protein NEDD8/RUB1 from the ECR1-AXR1 E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AK366297">
        <property type="protein sequence ID" value="BAJ97500.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951784.1">
        <property type="nucleotide sequence ID" value="XM_045095849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DQX9"/>
    <dbReference type="SMR" id="F2DQX9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541400"/>
    </dbReference>
    <dbReference type="GeneID" id="123401985"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0541400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401985"/>
    <dbReference type="HOGENOM" id="CLU_030988_6_0_1"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019788">
        <property type="term" value="F:NEDD8 transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000005">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="29"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ97500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="183" mass="20673" checksum="06F3CB3AFB96E816" modified="2011-05-31" version="1">MINLFKIKGQKKEEAANSNGGPPVKKQSPGELRLHKDIAELNLPKTTKISFPNGKDDLMNFEATLRPDEGYYLGGSFTFTFQVSPSYPHEAPKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNINTIIYGLNLLFSQPNDEDPLNHEAAAVLRDNPQKFQRNVQMAMSGGYVDNTHFPRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2DS31</accession>
    <name>F2DS31_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Homogentisate geranylgeranyltransferase</fullName>
            <ecNumber evidence="14">2.5.1.116</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123410571</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of tocotrienol (vitamin E). Catalyzes the condensation of homogentisate and geranylgeranyl diphosphate to form 2-methyl-6-geranylgeranylbenzoquinol. Possesses low activity with phytyl diphosphate as substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>homogentisate + (2E,6E,10E)-geranylgeranyl diphosphate + H(+) = 6-geranylgeranyl-2-methylbenzene-1,4-diol + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:38003"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:75411"/>
            <dbReference type="EC" id="2.5.1.116"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; tocopherol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.116" evidence="14"/>
    <dbReference type="EMBL" id="AK366699">
        <property type="protein sequence ID" value="BAJ97902.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959451.1">
        <property type="nucleotide sequence ID" value="XM_045103516.1"/>
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    <dbReference type="SMR" id="F2DS31"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740290"/>
    </dbReference>
    <dbReference type="GeneID" id="123410571"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0614050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0614050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0614050"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740290.1"/>
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    <dbReference type="UniPathway" id="UPA00160"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010356">
        <property type="term" value="F:homogentisate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010189">
        <property type="term" value="P:vitamin E biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13960">
        <property type="entry name" value="PT_UbiA_HPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homogentisate phytyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044502">
        <property type="entry name" value="AtHST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
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    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009525">
        <property type="entry name" value="PRK12887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009:SF6">
        <property type="entry name" value="HOMOGENTISATE PHYTYLTRANSFERASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009">
        <property type="entry name" value="HOMOGENTISATE SOLANESYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ97902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="397" mass="43715" checksum="55D23651ABB53B1D" modified="2011-05-31" version="1">MDSLRLRPSSLRSAPAARRRDHILPSFCSIQRNGKGRVSLSIQASKGPTVNPCKKFLDWKYSNHRISHQSINTSANAGQSLQPETEAHDTASFWKPISSSLDAFYRFSRPHTIIGTALSIVSVSLLAVESLSDISPMFLTGLLEAVVAALFMNIYIVGLNQLFDIEIDKVNKPTLPLASGEYSPATGVAIVSVFAAMSFGLGWVVGSPPLFWALFISFVLGTAYSVNLPYFRWKRFAVVAALCILAVRAVIVQLAFFLHIQTFVFRRPAVFSKPLIFATAFMTFFSVVIALFKDIPDIEGDRIFGIQSFSVRLGQSKVFWACVGLLEVAYGVAILMGATSSSLWSKSITVVGHAILASILWSCAKSVDLTSKAAITSFYMLIWRLFYAEYLLIPLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>F2DSE6</accession>
    <name>F2DSE6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448718</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK366814">
        <property type="protein sequence ID" value="BAJ98017.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981628.1">
        <property type="nucleotide sequence ID" value="XM_045125693.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981629.1">
        <property type="nucleotide sequence ID" value="XM_045125694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DSE6"/>
    <dbReference type="SMR" id="F2DSE6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379030"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379030.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379030.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379030"/>
    </dbReference>
    <dbReference type="GeneID" id="123448718"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0315280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0315280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0315280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379030.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379030.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448718"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="221"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="98"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ98017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="49693" checksum="B63F9E09742D7AC4" modified="2011-05-31" version="1">MDGKKKRNRKKKGNQGKNTGDAVTNADEAVPQSHNDDLAPKENYSGADADDAVSSVGEGTQYQNHEQTPHANQNVANVDETISPSVGEVIPCDENHEPTMTQENHKVSNTVYADQRSIGMSDSTVELDKDRLYEAKLDKLHDTIKQLEDEKGLWLQKMNKMESEIEKLHNKVGYHAQNEVILEEKLNNLQNGHDMLVKKEEVLDNKVKCVEDVNGVLTHQETFLKERLSGLEETNKALQEQVKVLDEASKSTVEENQQLLVSVDELESRLQTLEAKIALTEASITKEVPKNEVMNQTDLAGSFLHKQTAGFMTVISKGNELTADRGLNSSLVVTSDNIYSHVSNIPVGAYASDHADETSAHFPEATSSNGAGQSLMNANARQGFDEPRMSGEIVPVPLDDILIHEDDPQPAGSDVETAEEVPFTDAPIVGAPFRLISFVARYVSGADLVNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F2DVE7</accession>
    <name>F2DVE7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123439352</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms homooligomers and/or heterooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AK367865">
        <property type="protein sequence ID" value="BAJ99068.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372770">
        <property type="protein sequence ID" value="BAK03967.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972012.1">
        <property type="nucleotide sequence ID" value="XM_045116077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DVE7"/>
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    </dbReference>
    <dbReference type="STRING" id="112509.F2DVE7"/>
    <dbReference type="PaxDb" id="4513-MLOC_68994.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0230420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0230420"/>
    </dbReference>
    <dbReference type="GeneID" id="123439352"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0191170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0191170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0191170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0230420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0230420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0230420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439352"/>
    <dbReference type="eggNOG" id="KOG1623">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048643_4_0_1"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000033">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF222">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037238"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ99068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="314" mass="34283" checksum="7F30DD6B293B337B" modified="2011-05-31" version="1">MAAVGSPLIFAVGILGNILSFLVILAPVPTFYRVYKRKSTESFQSVPYAMALLSAMLWLYYALLTKDLLLLTINTVGCVVETAYLAIYLAYAPKQAKAFTAKLVCIMNVALYGAMVCVLQLLVRDGESRVTIAGGIGSAFALAVFVAPLAIIRQVIRTKSVEFLPFWLSFFLTISAVVWFFYGLLMKDFFVATPNVLGLLFGLAQMALHLVYKNPKKKGDVSEVQLPDDDEKNQLPLHHQQQQQAGTTGHVVAPPIIDDGEQVVNGSEDDVGGNKQQSVSVMDIVLPPPEEHPTLPPLDHPAPLPPMRMAVEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2DVP2</accession>
    <name>F2DVP2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440531</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367960">
        <property type="protein sequence ID" value="BAJ99163.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973031.1">
        <property type="nucleotide sequence ID" value="XM_045117096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DVP2"/>
    <dbReference type="STRING" id="112509.F2DVP2"/>
    <dbReference type="PaxDb" id="4513-MLOC_14036.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302260"/>
    </dbReference>
    <dbReference type="GeneID" id="123440531"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0252140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0252140.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0252140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302260"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="691043at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33257">
        <property type="entry name" value="OS05G0165500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33257:SF56">
        <property type="entry name" value="OS05G0335500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="184"/>
        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ99163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="184" mass="19677" checksum="F94473ED1FBC5C17" modified="2011-05-31" version="1">MSSSQRTLRDVMMQEERPTTMASRLLAPDASLSNPSFRNYYGVASAGSVPFLWESAPGTPKNDGVSSRALPPITPPPSYYTKTKIAKSESSKKLLSSSKPASFVPTMFRRSHTMPSPTASKECSRRKRLVASPPRSSFSSTSRGEDEEADGGAGSPTSTLCFRARRSGGGTGRLHGMIASVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWG8</accession>
    <name>F2DWG8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pseudouridine synthase</fullName>
            <ecNumber evidence="6">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123452724</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="6"/>
    <dbReference type="EMBL" id="AK368236">
        <property type="protein sequence ID" value="BAJ99439.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985370.1">
        <property type="nucleotide sequence ID" value="XM_045129435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWG8"/>
    <dbReference type="SMR" id="F2DWG8"/>
    <dbReference type="STRING" id="112509.F2DWG8"/>
    <dbReference type="PaxDb" id="4513-MLOC_44370.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519510"/>
    </dbReference>
    <dbReference type="GeneID" id="123452724"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452724"/>
    <dbReference type="eggNOG" id="KOG1919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_12_4_1"/>
    <dbReference type="OMA" id="EPPVMAC"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DWG8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000036">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="7">
        <location>
            <begin position="121"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ99439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="394" mass="43866" checksum="1B27E73B1A278F20" modified="2011-05-31" version="1">MAGALAGAGIVWQTPANPPEAQDYIFRNGRRYVRPYYFEFISHAKNRWAGKTIVDLFTDEFKGRPREYYVHAVKCGRLQVDEQMVQADYIVKSSQKISHFLHRHEPPVLGGNIVILQNEVDVVTVCKPASVPVHPCGQYRKNTVVGIMEAEHGLTPLFPVHRLDRLVSGLLIFAKSAERAERFRQQIEANLLQKEYVAKVVGVFPEGEQTVDANVNFNAREGRSTVEVSNGPGKAIPSGKQACTKFQRICTDGNHSIVLCKPVTGRTHQIRVHLKHLGYPIANDELYVSGNFCPRSSKGTSIHRATSLVCSLPSPDPDNSAEADLDFGIDPMCTNCPNLAPVGYDGDEEGLWLHCVRYTGPDWCYECPYPDWASLDNVAGKRMKSDIAFPTPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DYE1</accession>
    <name>F2DYE1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC123395162</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AK368910">
        <property type="protein sequence ID" value="BAK00113.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946035.1">
        <property type="nucleotide sequence ID" value="XM_045090100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DYE1"/>
    <dbReference type="SMR" id="F2DYE1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0535240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0535240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0535240"/>
    </dbReference>
    <dbReference type="GeneID" id="123395162"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0445350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0445350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0445350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0535240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0535240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0535240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395162"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000317">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000661">
        <property type="entry name" value="Serine-threonine protein kinase plant-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF73">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730843" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="32"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="802"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="830"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="BAK00113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0535240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1096" mass="120529" checksum="16C98896CA4970FF" modified="2011-05-31" version="1">MAAPFISSPIPIFTLVLLSAFSAFSPSPTTGSGSHTDLAALQAFKAQLADPHRILARNWTPSTSFCHWVGVSCSRHRQRVTALSFNGVPLAGSLAPHIGNLSFLSVLNLTRANLTGSIPAELGRLHRLRYLRLSRNSLSNAIPTSLGNLTRLEYIGLSLNKLWGQIPFEMLLHMHNLKVIALAANDLTGQIPPYLFNNTPSLTGIDFGNNSLSGPIPHTIATLSMLRFFSLQINQFSGLVPQAIYNMSSLQIMILTGNGNLTGMFPRNQSFNLPMLQQFSLDDNNFYGRFPVGLASCQHLQVIDLGGNSFVDVLPRWLANLPYLEQLFLGFSGLIGSIPVALSNITSLTDLDISNGNLTGEIPSELSLMHELSYMYLGGNQLTGKIPPSLGNLSNLYFLALGSNQLSGQVPTTIGKNSALNTLDLSNNNLDGNLDFLSSLSKCRELQILVIQSNYFTGILHGHMGNLSSQLITFAAGYNKLTGGIPTSISNITNLQRIDLSNNLFTEPISESITLLENLVWLDISHNEMLGPIPTQMGKLGSLQRLFLQGNKLLGSVPNNFGNLSSLEYVDLSNNHLSSMIPMTFFHLDKLIKLDLSHNCFVGPLPTDFSGLRQTNYMDISSNFLRGSIPNSLGELSMLTYLNMSHNSFNNSIPGPMEKLKGLASLDLSFNNLSGTIPMFLANFTYLTTLNLSFNSLEGQIPQGGIFLNLTSQSLIGNVGLCGATHLRFQPCLYRSPSTKRHLLKFLLPTLALAFGIIALFLFLWTRKELKKGDEKASVEPTDAIGHQIVSYHELIRATNNFSEDSILGSGSFGKVFKGRLNNGLVVAIKVLDMQLEQAIRSFDVECQVFRMVRHRNLIKILNTCSNLDFRALVRQYMPNGNLDILLHQSQSIGCLGFLERLGIMLDVSMAMNYLHHEHHELILHCDLKPSNVLFDEEMTAHVADFGIARLLLDDNSITSTSMPGTVGYMAPEYGLLGKASRKSDVYSYGIMILEVFTGRRPIDAMFGAQLNIRQWVHQAFPKEIVQVIDGQLLQGSSLSGCGLYNGFLESLFELGLACTTDSPDKRMTMSNVVVRLMKIKADYTKHATKMSPYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F2E5Q1</accession>
    <name>F2E5Q1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123407596</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371475">
        <property type="protein sequence ID" value="BAK02673.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956695.1">
        <property type="nucleotide sequence ID" value="XM_045100760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E5Q1"/>
    <dbReference type="SMR" id="F2E5Q1"/>
    <dbReference type="Allergome" id="1406">
        <property type="allergen name" value="Hor v 12"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651580"/>
    </dbReference>
    <dbReference type="GeneID" id="123407596"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0651580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407596"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000001">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF67">
        <property type="entry name" value="PROFILIN LP04"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK02673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="131" mass="14177" checksum="F5B6FD21642E56A0" modified="2011-05-31" version="1">MSWQTYVDDHLCCEIDGQHLTSAAILGHDGSVWAQSPNFPQFKPEEIAGIIKDFDEPGHLAPTGLFLGGTKYMVIQGEPGVVIRGKKGTGGITIKKTGMALILGIYDEPMTPGQCNLVVERLGDYLVEQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2E9G2</accession>
    <name>F2E9G2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123452354</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK372787">
        <property type="protein sequence ID" value="BAK03984.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984930.1">
        <property type="nucleotide sequence ID" value="XM_045128995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E9G2"/>
    <dbReference type="SMR" id="F2E9G2"/>
    <dbReference type="STRING" id="112509.F2E9G2"/>
    <dbReference type="PaxDb" id="4513-MLOC_4793.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0512500"/>
    </dbReference>
    <dbReference type="GeneID" id="123452354"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0425720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0425720.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0425720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0512500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0512500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452354"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_49_20_1"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E9G2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF73">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="344"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK03984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="485" mass="55257" checksum="6096D9622EBA1D71" modified="2011-05-31" version="1">MGRFRLTSLEARKVFVRMLSSGVGGSDAAVEAFDPAKRLCKLIISCRQASGLEIELDHSDLRVTPDVAERVLERLDNAGMLAYRFFEWARKQRRGGGCAHTVRSFHTVVASLAKIRQYQLMWDVVAIMRRQGVVNVETFGIIMRKYARAQKVDEAVYTFNVMEKYGVVPNLAAFNSLLCALCKSKNVRKAQEIFEQMNGRFSPDAKTYSILLEGWGRAPNLPKMRKVYSDMLDAGCQPDIVTYGIMVDSLCKTGRVEEAVFVVQDMTSRGCQPTTFIYSVLVHTYGVEMRIEDAVATFLDMQKDGIVPDVVVYNALVTAFCKVKKFDNAFRVMDDMEGHGITPNSRTWNIILNKLISLGKDDEAYRVFRRMIKRCQPDSDTYTMMIKMFCENDRLEMALKVWKYMRLKQFLPSMHTFSVLINGLCDKGEVSQACVLLEDMIEKGIRPPGSTFGKLRQLLLKEGRKDVLEFLVDKMKILIQEPLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFS9</accession>
    <name>F2EFS9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123425948</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Flower</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AK375006">
        <property type="protein sequence ID" value="BAK06201.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965656.1">
        <property type="nucleotide sequence ID" value="XM_045109721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFS9"/>
    <dbReference type="SMR" id="F2EFS9"/>
    <dbReference type="FunCoup" id="F2EFS9">
        <property type="interactions" value="1435"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2EFS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142660"/>
    </dbReference>
    <dbReference type="GeneID" id="123425948"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0117500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0117500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0117500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0142660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425948"/>
    <dbReference type="eggNOG" id="KOG0201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EFS9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000207">
        <property type="entry name" value="serine/threonine-protein kinase dst1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="317"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="511"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="646"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="515"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="646"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK06201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="692" mass="76096" checksum="3B3E2ADCD7159604" modified="2011-05-31" version="1">MSDSASMAAATEARFSNRDLIGRGSFGDVYRGFDKELSKEVAIKVIDLEEAEDDIEDIQKEISVLSQCRCPYITDYYGSYLHQTKLWIVMEYMAGGSVADLLQAGPPLDEISIACILRDLLHAVEYLHSEGKIHRDIKAANILLTESGDVKVADFGVSAQLTKTMSRRKTFVGTPFWMAPEVIQNSDGYNEKADIWSLGITAIEMAKGEPPLADIHPMRVLFMIPRENPPQLDEHFSKPMKEFVSLCLRKNPAERPSAKELLKHRFIKNARKTPKLLERIRERPKFTVKGSINATQNGQTHIEEDDYGGTIKVERNTKHAASPSSQGTVRKAAGWDFPDRSEGTGTVRAGLRPPQITSTKDGRFDMPQNPSTLKRATDRENQWRTSGTGSEESSSTNMSKKEAQTDHGRLESSTEYNDQSVSGSGTVVLRSPRAPQVYSAGPNHSSKPPSRFSSYEDMSISGTVVRNQSEEAETPRSSRSRLGTQEKTSNASLEDSATNLAEAKAALQAGFRKGNARERPATNKHEKESQEPRSSVVNSHEVRSENVDSQKGRKSRQPSDGQSAPRASAASPALSSLIIPSLKEATGDKYEGPVVHAVLSSLMDLEHEIPGSCEVLVGRILHRLGSSKDSSLQSLNETAISIFTKKPEPPLEAESNKKQASTPPLAAPTVSPLARFLLTRWQNQVSQDLNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKS3</accession>
    <name>F2EKS3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123444389</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Flower</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transcriptional activator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK376751">
        <property type="protein sequence ID" value="BAK07945.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977018.1">
        <property type="nucleotide sequence ID" value="XM_045121083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKS3"/>
    <dbReference type="SMR" id="F2EKS3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0288270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0288270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0288270"/>
    </dbReference>
    <dbReference type="GeneID" id="123444389"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0239300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0239300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0239300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0288270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0288270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0288270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444389"/>
    <dbReference type="HOGENOM" id="CLU_045755_0_0_1"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016679">
        <property type="entry name" value="TF_GATA_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF45">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016992">
        <property type="entry name" value="TF_GATA_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 8" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR016992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAK07945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0288270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="392" mass="41354" checksum="99A4984FFA4A5E4B" modified="2011-05-31" version="1">MEATATAPDCDAHGYYGRDNKKGPDLFVVDDLLALPCDDDEEDEEAPPFLPAATAVVKQEAAGFGNASADSSTVTALDSCSNSFSGLADGDFSGGLCEPYDQLAELEWLSNYMGEGEESFATEDLHKLQLISGIPSGGFPTANGPPAPATTAAASASASATAQPGVFLPEGPVPAKARSKRSRVAPGNWSSRLLVLPPAPASPPSPASMAISPAESGVSAQAFHVKKPSKPAKKKEVPPQAQPVTVSSPTAPSGVTAAANEGRRCLHCETDKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVTSRHSNSHRKVLELRRQREMHHQPSQHLQQHVVPGGVGRIMHMESHLLFDGPAAPPIIGGGDDFLIHHRLGTTDYRQQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0XFC5</accession>
    <name>M0XFC5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949772.1">
        <property type="nucleotide sequence ID" value="XM_045093837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XFC5"/>
    <dbReference type="SMR" id="M0XFC5"/>
    <dbReference type="FunCoup" id="M0XFC5">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XFC5"/>
    <dbReference type="PaxDb" id="4513-MLOC_59545.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530900"/>
    </dbReference>
    <dbReference type="GeneID" id="123399428"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0441640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441640.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399428"/>
    <dbReference type="eggNOG" id="ENOG502QVP8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XFC5"/>
    <dbReference type="OMA" id="WFLEGID"/>
    <dbReference type="OrthoDB" id="595207at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF70">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530900.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="48800" checksum="89C32735D410EFAF" modified="2013-04-03" version="1">MGSMGNVRIQSRRMVRPEPTASRLPEPETTIHLTPWDLKHITVEHIQKGVLLPKPPTTGGHVDVERLASSFARALGRFYPYAGRLAVSVAPHSQSLTISLRCSGEGAEFIQAVAPGVTVADVTASLVVPRVVWSFFPLDTLLGADAAAGTRPVLAAQVTELADGVFIALTMNHGVADGTTFWHFFNTWSELSRAGDDVMISTPLPVFDRSFLGSSCAVPIPLPFGKLEDVVGQRVHVFPPVQECFLHFSAASVKELKAKANAEVSSSGTDSTISSLQALLAHLWRAVCRARQLTPEQETAYIVLVGFRGRVGGIPASYAGNAVGNVTAKSTAGDIVGKGLGWAAWLLNRAVASWDEASTRDKLASWPQEPSFTSLPAFVAAVPTGTFTGSSPRFDVYGNDFGWGAPVAVRSGSGNKVDGKVTVYAGRGGGGSMALEVCLAPEALARLVADEEFMNATSTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0XRJ5</accession>
    <name>M0XRJ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Malonyl-CoA decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123397254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044947811.1">
        <property type="nucleotide sequence ID" value="XM_045091876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XRJ5"/>
    <dbReference type="SMR" id="M0XRJ5"/>
    <dbReference type="PaxDb" id="4513-MLOC_62375.5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478610"/>
    </dbReference>
    <dbReference type="GeneID" id="123397254"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0396570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0396570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0396570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0478610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397254"/>
    <dbReference type="eggNOG" id="KOG3018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CEQFAPD"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XRJ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050080">
        <property type="term" value="F:malonyl-CoA decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001294">
        <property type="term" value="P:malonyl-CoA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.90:FF:000002">
        <property type="entry name" value="Malonyl-CoA decarboxylase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.150:FF:000002">
        <property type="entry name" value="malonyl-CoA decarboxylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.150">
        <property type="entry name" value="Malonyl-CoA decarboxylase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.90">
        <property type="entry name" value="Malonyl-CoA decarboxylase, oligemerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038917">
        <property type="entry name" value="Malonyl_CoA_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007956">
        <property type="entry name" value="Malonyl_CoA_deC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042303">
        <property type="entry name" value="Malonyl_CoA_deC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035372">
        <property type="entry name" value="MCD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038351">
        <property type="entry name" value="MCD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641:SF1">
        <property type="entry name" value="MALONYL-COA DECARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05292">
        <property type="entry name" value="MCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17408">
        <property type="entry name" value="MCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malonyl-CoA decarboxylase N-terminal" evidence="3">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Malonyl-CoA decarboxylase C-terminal" evidence="2">
        <location>
            <begin position="205"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="55951" checksum="E6B6CDF70416BC76" modified="2013-04-03" version="1">MTRSHTPKSLAVLLRARMHPDSIPSPLPAPSPPASPDPNPSARPAAASSVRHWLHASISAASPPPAALECFSDGYRSLDRGGRHEILRSLATDYDVPRARVRDLMRQYLSVSATGEDGDEQPRAEREEGGAASALYRMERGLRDALRPKHAGFLEAMNAQPGGLKLLAVIRADLLALLGEENAPVLRALDGYLKEKLVTWLSPAALALHQITWDDPASLLEKIVAYEAVHPIRNLIDLKRRLGVGRRCFGYFHPAIPGEPLIFIEVALLKDMATSIQEVLLDVPPIAECEAKCALFYSISSTQPGLSGINLGKFLLKRVIDMLRKDMPSVQIFATLSPIPGFMQWLLAKLASQIKLAEAEMQEGNSLEGASSTFRESILFPEEEKMIHSAIDQVDGKQGIELLQDILKSSQWVKSDKLSAALKSPLMRLCARYLTREKIRGKALDAVANFHLQNGAMVERINWMADQSEKGIEQSGGIMVNYLYRLENIEEYASSYSATGLIHTSPSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>M0YB80</accession>
    <name>M0YB80_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123448213</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980975.1">
        <property type="nucleotide sequence ID" value="XM_045125040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YB80"/>
    <dbReference type="SMR" id="M0YB80"/>
    <dbReference type="FunCoup" id="M0YB80">
        <property type="interactions" value="1893"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0YB80"/>
    <dbReference type="PaxDb" id="4513-MLOC_67497.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0080590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0080590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0080590"/>
    </dbReference>
    <dbReference type="GeneID" id="123448213"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0065890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0065890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0065890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0080590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0080590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0080590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448213"/>
    <dbReference type="eggNOG" id="KOG0356">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YB80"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YB80">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0080590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="581" mass="61366" checksum="5FF94E88F59B4C89" modified="2013-04-03" version="1">MYRAAAAAISRSSSSALRRHLARGGGGGGEQRQWARGYAAKEVTFGVGARAAMLRGVNDLADAVKVTMGPKGRNVVIERPNRSPKVTKDGVTVAKSIEFEDSAKNVGASLVKQVADATNKAAGDGTTCATVLTQAILTEGCKAVAAGVNVMDLRNGINKAISAVTAHLKSKAWMIDSPDEINQVATISANGEKEIGDLISKAMGIVGKDGVITIADGKTLDNELEAVQGMKLTRGYISPYFVTDQKTQKCELKNPLILIHDKKISSMNSLLPALQISIQNNRPLLIFAEDVEGEALSMLVLNKHRAGLKICAVKAPGFGENRRANLDDMAILTGGQVISEEQGLDLDKVELQMLGTAKKVTVSLDDTIILDGGGERQQIEERCQQLRESLENNTAMFDKEKAQERLSKLSGGVAVLKIGGASEAEVGEKKDRVTDALNAARAAVEEGIVPGGGVALLYAAKELDQISTSHEDERIGVQIIKNALKAPLMTIAANAGVDGAIVIGKLLEQDNLSLGYDAAKGEYVDMIKAGIIDPVKVIRTALQDAASVSLLMTTTEAAVAELPSTKARIASRMPDMSGMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>M0YX56</accession>
    <name>M0YX56_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123434210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044971434.1">
        <property type="nucleotide sequence ID" value="XM_045115499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YX56"/>
    <dbReference type="SMR" id="M0YX56"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0049610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0049610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0049610"/>
    </dbReference>
    <dbReference type="GeneID" id="123434210"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0038720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0038720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0038720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0049610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0049610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0049610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123434210"/>
    <dbReference type="OMA" id="MAYKLEA"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YX56">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF81">
        <property type="entry name" value="ENDOPHILIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0728">SH3 domain</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="146"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0049610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="369" mass="41268" checksum="AD9C69C2AF8646AD" modified="2013-04-03" version="1">MEAIRKQASKLREQVARQQQAVLKQFGGGYADSVFADEGEAQQHSKLEKLYISTRAAKHFQRDIVRGVEGYIVTGSKQVELGNKLCEDGKKYGVENTCTSGSTLSRAALSFAKARSLMEKERGNLLKAFGTQVAEPLRAMVMGAPLEDARHLAQRYDRMRQEAEAQAIEVSKRQMKLRETSGNGDMISRLEAAESKLQELKSNMGTLGKEAVAAMTAVEAQQQRLTLQRLIAMVESERSYHQRVLQILDQLEREMVAERQRIEGAPPPVIENSMPPPPSYEEVNGIFMRTPTVAELVETVEHFLAEAIQSYRAETETELNLATGDYIVVRKVSNNGWAEGECRGKAGWFPSEFIEKRDRVLASKVAQVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q3T5P2</accession>
    <name>Q3T5P2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">HvCBF7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CBF7</name>
        <name evidence="12" type="synonym">LOC123402106</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2005" name="Plant Mol. Biol." volume="59" first="533" last="551">
            <title>Structural, functional, and phylogenetic characterization of a large CBF gene family in barley.</title>
            <authorList>
                <person name="Skinner J.S."/>
                <person name="von Zitzewitz J."/>
                <person name="Szucs P."/>
                <person name="Marquez-Cedillo L."/>
                <person name="Filichkin T."/>
                <person name="Amundsen K."/>
                <person name="Stockinger E.J."/>
                <person name="Thomashow M.F."/>
                <person name="Chen T.H."/>
                <person name="Hayes P.M."/>
            </authorList>
            <dbReference type="PubMed" id="16244905"/>
            <dbReference type="DOI" id="10.1007/s11103-005-2498-2"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">20 DAP spike tissue</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2006" name="Theor. Appl. Genet." volume="112" first="832" last="842">
            <title>Mapping of barley homologs to genes that regulate low temperature tolerance in Arabidopsis.</title>
            <authorList>
                <person name="Skinner J.S."/>
                <person name="Szucs P."/>
                <person name="von Zitzewitz J."/>
                <person name="Marquez-Cedillo L."/>
                <person name="Filichkin T."/>
                <person name="Stockinger E.J."/>
                <person name="Thomashow M.F."/>
                <person name="Chen T.H."/>
                <person name="Hayes P.M."/>
            </authorList>
            <dbReference type="PubMed" id="16365758"/>
            <dbReference type="DOI" id="10.1007/s00122-005-0185-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">20 DAP spike tissue</tissue>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AY785864">
        <property type="protein sequence ID" value="AAX23704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY785865">
        <property type="protein sequence ID" value="AAX23705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY785866">
        <property type="protein sequence ID" value="AAX23706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY785863">
        <property type="protein sequence ID" value="AAX28954.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3T5P2"/>
    <dbReference type="SMR" id="Q3T5P2"/>
    <dbReference type="STRING" id="112509.Q3T5P2"/>
    <dbReference type="PaxDb" id="4513-MLOC_3095.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0498060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0498060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0498060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0600830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600830"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW5S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063331_0_2_1"/>
    <dbReference type="OMA" id="CEANTCS"/>
    <dbReference type="OrthoDB" id="1932364at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051032">
        <property type="entry name" value="AP2/ERF_TF_ERF_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985:SF72">
        <property type="entry name" value="OS02G0657000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="48"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAX23704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAX28954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="219" mass="22994" checksum="2710479ED9A9A6C4" modified="2005-10-11" version="1">MGATEDSSSGSETTTSSSVEALASPPSPTATTASSKKKRARNDGRHPTYRGVRMRSWGKWVSEIREPRKKSRIWLGTFATAEMAARAHDVAALAIKGRAAHLNFPDLAHELPRPATAAPKDVQAAAALAASADFPASATATANAGAKNPDGPEPNAASASTPPDTAEDALFDLPDLLFDLRHGPPSCQVSCASTWDDDVAFAGPGAGVFRLEEPLQWEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287DZZ7</accession>
    <name>A0A287DZZ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123395300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946216.1">
        <property type="nucleotide sequence ID" value="XM_045090281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287DZZ7"/>
    <dbReference type="SMR" id="A0A287DZZ7"/>
    <dbReference type="PaxDb" id="4513-MLOC_16702.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468700"/>
    </dbReference>
    <dbReference type="GeneID" id="123395300"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0468700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395300"/>
    <dbReference type="eggNOG" id="KOG1803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287DZZ7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF17">
        <property type="entry name" value="OS09G0130800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="7">
        <location>
            <begin position="504"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="8">
        <location>
            <begin position="729"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="980" mass="107594" checksum="BCF34BE8416154CA" modified="2017-11-22" version="1">MAMKHAKYQFLFSSSATVLHPSRRELKILSSPSFLSSTSSAPISFLQQGHLRLVPSAGPTEQVLLPPDSHSTTPNGTSSDGNRNKGRRSRRKRKETAKEEKECVPSAEEVSIRIAEAYESGDPLGRKELGRCVMQWLKLGMCSMASTFASTEVQNDGAAFSLDGGSSEGSLEFMILAQPYLSATPMPKGHEALCLKASTHYPTLFDHFQRELREVLLKHQNQGRISDWRSTESWMLLKKLAKSAQHRQGARKPKVPAMHNLLGISLDKTRLMQTKIEDFVKKMSDLLNIERDAELEFTQEELNAIPTTDGAAKQPLKPVEYLVSHGQAEQEQCDTVCNLNVISTSTGLGGLRLVLFRVEGTHKLPPSTLSPGDMVCVRTCNNQGEVGTSCVQGFIHNLGEDGCSITVSLKSRPGDPTFSKFSGKSVRIDRIQALADGLTYERNCEALILLQRKGLQKTNASIGVVATLFGDKEDMTMLEQNNLTDWGESAIQDAGLLRKHKYDFDASQSQAISLGLNKKRPVLVIQGPPGTGKTGLLSYLIDCAVQQGERVLVTAPSNAAVDNMVEKLSGTGLNIVRVGNPARISPSVSSRSLGELVKRRLEKFTQEFQRKKSNLRKDLKQCLQDDSLASGIRQLLKKLGKNFRKKENEIIKEVLSNADVVLSTNIGAADPLIKEIGFFDLVIIDEAGQAIEPSCWIPILRGKRCILAGDQHQLAPVILSREAMQGGLGMSLLERASSLHDELLTTKLTMQYRMHESIANWASNEMYFGLLKSSPSVASRLLVDYPSIQETWITRCALLLLDTRMPYGSLNIDCEENLDLAGTGSFYNSGEADIVAQHVVNLVLCGVSPTSIAVQSPYIAQVQLLRDRLEEYPEALGVEVSTIDSFQGREADAVVISMVRSNSLGAVGFLGDNRRMNVAITRARRHVALVCDSSTICNNEFLARLLRHIRQHGQVRHVEPGSYGGDYGLGFSPPALPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287FGY7</accession>
    <name>A0A287FGY7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine rich repeat malectin kinase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287FGY7"/>
    <dbReference type="SMR" id="A0A287FGY7"/>
    <dbReference type="STRING" id="112509.A0A287FGY7"/>
    <dbReference type="PaxDb" id="4513-MLOC_25304.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0051780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0051780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0051780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0040530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0040530.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0040530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0051780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0051780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0051780"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287FGY7"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287FGY7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0051780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11870" checksum="FA5C1C9872CDD69E" modified="2017-11-22" version="1">MGRIGVTVPRTLSGLTRLQELYLEGNTLAGGVPGKVLSRMSSLRYLSLAGNRLEGTLSPELSDVRSLVRGAPRRFRGLMTRTFSLLLRCSFVMLCTAVDRNPGEKCRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287L2G3</accession>
    <name>A0A287L2G3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123439948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972498.1">
        <property type="nucleotide sequence ID" value="XM_045116563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287L2G3"/>
    <dbReference type="SMR" id="A0A287L2G3"/>
    <dbReference type="FunCoup" id="A0A287L2G3">
        <property type="interactions" value="1125"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287L2G3"/>
    <dbReference type="PaxDb" id="4513-MLOC_53485.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270400"/>
    </dbReference>
    <dbReference type="GeneID" id="123439948"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0224100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0224100.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0224100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0270400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270400.1.CDS1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123439948"/>
    <dbReference type="eggNOG" id="ENOG502QR2Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287L2G3"/>
    <dbReference type="OMA" id="RDAHVIN"/>
    <dbReference type="OrthoDB" id="1885092at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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        <property type="entry name" value="Sensory_adhesion_molecule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31460:SF3">
        <property type="entry name" value="MESOCENTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="34039" checksum="72338EDC1B0D6C4D" modified="2017-11-22" version="1">MAASYIALLLILAAVAPRPSSARHIVTFTPSRGVSPSSLAWDPTAQHFVVAGGGDAVLSVSDAGVTESIASPGASAVAVDDRRRRLIVASAGSVSAFDLRTPRPHRFLFSTTLPDPAAPGGVAVDPQSGVVFLTLGAQIYRLSPDGDLAALPASPVYGSEPLSSLTAHVSRGFLLVGRPSTGHLLRVDMEDGTARAVSGPFTPPTPVAIAVRSDGMVAVGGTATLRLVGSNDGWVSCAEHDVGEPDGAVAAVAVAVRERKRVYALVEAEVEGKREWRIEEVEWKKEDEGEMVAVLILVGAALAIFMFWRFQMRQLAGNMNKKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287Q7L6</accession>
    <name>A0A287Q7L6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Neutral ceramidase</fullName>
            <ecNumber evidence="14">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123395581</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="14"/>
    <dbReference type="RefSeq" id="XP_044946534.1">
        <property type="nucleotide sequence ID" value="XM_045090599.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946535.1">
        <property type="nucleotide sequence ID" value="XM_045090600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287Q7L6"/>
    <dbReference type="SMR" id="A0A287Q7L6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421350"/>
    </dbReference>
    <dbReference type="GeneID" id="123395581"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0350760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0350760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0350760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395581"/>
    <dbReference type="OMA" id="GRETWVP"/>
    <dbReference type="OrthoDB" id="191371at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287Q7L6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2300:FF:000002">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF19">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="15">
        <location>
            <begin position="41"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="16">
        <location>
            <begin position="619"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="783" mass="86037" checksum="446F929B3F6C23A8" modified="2017-11-22" version="1">MEASSCLRYQFCGFGLSWIWLCLLLLRLFQNCSPVLSESPYLVGLGSYDITGPAADVNMMGYANAEQVTAGIHFRLKSRAFIVAEPDDGKRVVFVNLDACMASQLVTIKVLERLKVRYGDLYSENNVAISGIHTHAGPGGYLQYVVYIITSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIYVNKGDLLDAGVNRSPSGYLNNPAEERSKYRYNVDKEMTLVKFVDDEFGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWAEQNGLPKQTGHANSDDFGSLHLPRRVSTIIPEPDEITDDLMQLASSYEASGGRILAGSNITRRIRSTQEKNAKFVSAFCQSNCGDVSPNVLGAFCIDTNLPCDFNHSTCNGKNELCYGRGPGYPNEFESTRIIGNRQFLKAVDLFNSASEELQGKIDYRHTYLDFSQLEVSVSTSTGGQQVVKTCPAAMGFSFAAGTTDGPGAFDFKQGDDKGNPFWRLVGGILKKPGKEQVECQAPKPILLDTGEMKEPYDWAPAILPIQIIRIGQLVILSVPGEFTTMAGRRLRDAIKNVLISGSNGEFNSNTHVVLAGLTNTYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQAFQKLATAMIENKEIPATNILPPDMLDKQIGLLPGVVLDSTPPGVHFGDVSSDVEANSDFRKGSTVNATFHSACPRNDLLTDGTFALVERLNGNDWIPVYDDDDWSLQFKWSRPSKLSPESFATLEWTIPEDAAPGVYRLRHFGASKALLGSIEHFTGTSRAFAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W8I8</accession>
    <name>A0A8I6W8I8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger-XS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123451738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984209.1">
        <property type="nucleotide sequence ID" value="XM_045128274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W8I8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091850"/>
    </dbReference>
    <dbReference type="GeneID" id="123451738"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0091850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451738"/>
    <dbReference type="OrthoDB" id="706267at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Znf-XS_domain"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="145"/>
            <end position="185"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="52"/>
            <end position="118"/>
        </location>
    </feature>
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        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="21001" checksum="AD3DD22C2DBBFD34" modified="2022-05-25" version="1">MEGARRAAAVADVEKEMPKQEDTTATVVVAADLEKELPKQEGTTATVVAAADLEKEVPKQEDTTSSGVVKAHDDEEREAAGMKRKLAKTGFKSPYDSDYDDGDPYEYNSGDDVEDGNKESFIEKEAQKIRKKGKAAYYKSGMFRCPYCITKPKPKDGIYEHLMSHARGLAASVADIKTRAEHATLLKALGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WAA6</accession>
    <name>A0A8I6WAA6_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123430374</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044970502.1">
        <property type="nucleotide sequence ID" value="XM_045114567.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WAA6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000130"/>
    </dbReference>
    <dbReference type="GeneID" id="123430374"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0000280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0000280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0000280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0000130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430374"/>
    <dbReference type="OMA" id="KPGINAC"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF59">
        <property type="entry name" value="PAIRED AMPHIPATHIC HELIX PROTEIN SIN3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="9">
        <location>
            <begin position="448"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="790"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="855"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="792"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="828"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="925"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="945"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="971"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1331" mass="150327" checksum="FFBD9AA9AA319E76" modified="2022-05-25" version="1">MSPPPASPALSAQKLTTNDALAYLKAVKDKFHDNRAKYDEFLEVMRDFKSARIDTAGVIIRVKTLFSGYPELILGFNAFLPKGFAIKLQDIDGGDKKPVDFMEAINFVNKIKARFQAQDHVYKSFLAILNMYRMHNKSIQDVYQEVATLFREHADLLEEFKHFLPDTSNAPQQLAAPKAVSAKQEAPAMPSASARNVQSIKRERPHPSTAERDSSVDRPDLEHDPDRKRIDKEKDRKIDRDRKDHEKDGEYESKDLDGGLRKRKAFSKKLEGETHQGAASISASSYNDNDALKGAYTQEFLFCEKVKEKLEPEAYQEFLKCLHIYSQEIITRSELKNLVHDILQRYPELMNGFSEFLEHCENIDGFLDGVINKRQTSRTVKALEKERDKGRAGEDRERDAEKLSEKERERLDKVSALNSKEATTHKATAFSTKEKYLCKPISELDLSNCQRCTPSYRLLPKNYPVPASSCRTDLGVSVLNDHWVSVTSGSEDYSFKHMRKNQYEESLFRCEDDRFELDMLLESVNVAIKRVEELIEKMQENSIKPDSPIRIDEHLTSLNLRCIERLYGDHGLDVMDVLRKNASVALPVILTRLKQKQEEWSRCRSDFNKVWAEIYAKNYHKSLDHRSFYFKQQDTKNLSTRALLCEIKEINEKKRKEDDVLLAIAAGNRRPIVPNMSFEYADPEIHEDVYQIIKYSCGEVCGSTDQAEKVMRTWTTFVEPILGVQPRTPVVEDAGVVKSKSRTPTTGLASVGESNNVIPNGAVTVKQANGDESIPKEQAQSSRGLLANGVSEDAHNGFHDADRTVRRGEGPSNTSLHQTEQNQRRTNMELTSGTNSSRSNFSGNEAVVEAMGGNETIPSIGRGEAGRPGPIPQKNPKVEREEGELSPNGDFEEELYRPLGNAAVGGASKPKEVSAGRTRPAEFAGENDADADDEGEESAQRSTEDSENASEAGEDASGSESGDGGECSPEDHEDEEDVDHDDPDAKAESEGEAEGNTEAHDVDGGMTLPFSERLHNTVKPLAKHVPRALQDHGGKFSRIFYGNDSFYVLFRLHQILYERILSAKTNSSTAEKKWRTSKDTSSPHQYSKFMSALYNLLDGSSDNTKFEDDCRSIIGTQSYVLFTLDKLIYKVVKQLQAIAADEMDNKLLQLYVYEKSRSPGRFFDLVYHENARVLLHDESIYRFERRSNPMRLTVQLMEYGHEKPEITAVSIDPNFSSYLYDEYLSSVSDSKVSEDVFLERNKRKRGSSNGPQASLEVMDDFKVANGLECKITCKSSKVSYVLDTEDFLFRMRNRRRISSGPTTQAPGKADFVKPADAVKAQRFHKFLLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WFC5</accession>
    <name>A0A8I6WFC5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123430398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970203.1">
        <property type="nucleotide sequence ID" value="XM_045114268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFC5"/>
    <dbReference type="SMR" id="A0A8I6WFC5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174390"/>
    </dbReference>
    <dbReference type="GeneID" id="123430398"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0144130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0144130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0144130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430398"/>
    <dbReference type="OrthoDB" id="692882at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042885">
        <property type="entry name" value="HIPP47/16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932:SF5">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="12527" checksum="141F3A36879FA7AF" modified="2022-05-25" version="1">MKQKMVIRVHMTCDKCRSKAMGLVASVHGVERVEIQGDDRDRLAVVGDGVDAANLTACLRKKVGSADLLTVEAVVPEKKPAAASQTTEAPCPQQWYPGYYSWPAGAYSHCYPYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WFH4</accession>
    <name>A0A8I6WFH4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123430375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970178.1">
        <property type="nucleotide sequence ID" value="XM_045114243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0040800"/>
    </dbReference>
    <dbReference type="GeneID" id="123430375"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0031690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0031690.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0031690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0040800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0040800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430375"/>
    <dbReference type="OrthoDB" id="690835at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="119" mass="12304" checksum="961F4F5C8A5E412F" modified="2022-05-25" version="1">MSKPPPPPPTPPASEISSSVAAAAPRSLPPTLLLPPSSSGNRSARGGSAAGSRKGKAAGAERGGHQHDPLVEAVRLVGRDVDPGVAGADILELAMAKGPMFSWLRYWPEEGYPEEGRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIT4</accession>
    <name>A0A8I6WIT4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exportin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123431075</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970842.1">
        <property type="nucleotide sequence ID" value="XM_045114907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIT4"/>
    <dbReference type="SMR" id="A0A8I6WIT4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0042990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0042990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0042990"/>
    </dbReference>
    <dbReference type="GeneID" id="123431075"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0033380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0033380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0033380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0042990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0042990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0042990"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000287">
        <property type="entry name" value="Exportin-4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF1">
        <property type="entry name" value="EXPORTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0042990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1160" mass="129206" checksum="523AAB2C63B4ABC5" modified="2022-05-25" version="1">MQGFPGGVPDPQQLQATMLAIEQACSLIQVHMNPAEAEKVLSSLHSSLMPYQACRFILETSLMPNARFQAAGAIGDAAIREWGILTDDNKRSLILYCLNYVMEHAGSPDGYVQSKVSAVAARLLKRGWLEFPDQEKGAIFFEVEQSIRGMHGPNRQFAGINFLETLVSEFSPSTASSMGLPKEFHDQCQLSLEVKFLKDFYGWAQAAVFNTADKVLNSNVTIPEEKACSAALRLMLQILSWSFKPTLEHENLDAKIKSGLRSDAINLRKFERSLVKPGSSWTDILISSAHTTWVLNFYTTLRQKYSYDTLWGDSPIAVSCRQLIVQLCSLAGAVFPNDNGDAQIEHFMHILSAVILWIEPPNVIAESIRNGGSESEFIDGCHVLLSVASLTSSSLFDNLLKSIRQYGTVNLLSALTSEAVKSVLDNQNEEETWGSDALDILLETWNVILGEACADKSPMSADGALAASSLFKIIVESHLKAAADSAFEDSDDAEYFHVSVSKRDEQLALYALIARAAADTTIPFLEQLFSERFARLSQRDVENDPTRTLEELYWLLLITSHVLTDSGEGETLLIPEALQAGFTNVVEVAQHPVVTLSWSIINFSRQCLDPGIRGRYFSPRLMEAVIWFLARWVATYLVPLDVSREIDSVGNHGSQHSRKLLNSFAWDNNQGELVLDFVVLMSMVALTTYQGEIELQTLTCQKLLASVVRRKHTCAYVVQLDSWRDLTRAFASGRSLFSLSGRLQRSLAETLACAASCIKDPEASVQYLRDLMGPVAGCLVENASRSDLKSVAHQPDVIYMVCCLLERLRGAARATQPRTQKVLFEMGHTVMNSLLTLLEVYKNQSEVIYMILKFVVDFIDGQAVFLDGKETSVLMSFCLRLLQIYSSHNIGKVMLSLSSTLRSESQSEKYKDLRALLRLLTNICSKDLVGFLSDSNIEGSPDIAEVIYVGLDIVTPLISLDLLKYPKLSRDYFVLMSHLLEVYPEKVAHLNRDAFGRITGSLEFGLRNQDGDVVERCLAAVNALASYHFKERLGGRGGLGSQVMESEGSNGKLQESITSHFLRLLLQLLLFEDFRMELAGSAADALLPLLFCEQELYQRLVHELLEKEQNPTVKSRLSLAFHNLTSSNNLSSTLDRPNRQKFRKNLRVFLGEVSGFMQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJM2</accession>
    <name>A0A8I6WJM2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJM2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0095390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0095390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0095390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0095390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0095390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0095390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0095390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="26577" checksum="8C1BD5A883FEE212" modified="2022-05-25" version="1">MFSNLPREMNILFYTIHIYMHSLVPYFSVFLVTLTCLFPTMDFDSEDESESEDEQEPIIPPVAKENGKPEAKEQKKQVLIDTGSSKSKAAAKDIGKSKKDDDSDDDGSDDDDSEDDSADGALIPMGDDSDDSEEGDEDSDSDEDSSDEEEEETPKQETGKKRAAGSDIKTPVADKKAKIATPSGQKTGDKKGAVHVATPHPAKKVGKTPATSDKSPKSGGSVACKSCTKTFNSEVALASHAKAKHAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WN20</accession>
    <name>A0A8I6WN20_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC123429257</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044969248.1">
        <property type="nucleotide sequence ID" value="XM_045113313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WN20"/>
    <dbReference type="SMR" id="A0A8I6WN20"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0104560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0104560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0104560"/>
    </dbReference>
    <dbReference type="GeneID" id="123429257"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0085830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0085830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0085830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0104560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0104560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0104560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429257"/>
    <dbReference type="OrthoDB" id="2013775at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051809">
        <property type="entry name" value="Plant_receptor-like_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008">
        <property type="entry name" value="OS04G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008:SF284">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 21" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035163549" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="33"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="756"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0104560.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1055" mass="114760" checksum="C13F765F3C8763F3" modified="2022-05-25" version="1">MAPAGTLRSLPPHGVLILFTHLLIFMSSNTVAKSSINGSGIDMQALISFKRSIHYDPLGVLESWNRSIGFCRWTGVTCGTTLPPRVVSLNLSAASLSGQFSPWVANLTSIQLVDLSYNGFSGAIPEELGTLQHLRYLELRTNSLVGIIPRSLGTCRSLREVDLSSNILEGTIPDFHRLPALQTLILSRNDLIGTIPRSLGNSRSLKNIDLSSNFLVGPIPEFDKMSALQILDLSSNDLSGSIPSSLGNVSSLIEIRLDTNSLSGAIPETLSLVQNLSVLNLANNSLSGHLSAKLCNISSLIYLDLAQNKLTGSIPPSIGNTSPNLERLSMSGNEFKGLIPSSLANASYLRRIDLGHNSLVGPVPSLGSLSDLRVLNLESNFLQAEGWEFLVSLTNCAQLQTLDMSGNALNGNIPISVGNLSRSLVRLSLGNNQIVGTIPIEIFNLRNLQMLAMGQNNFLSGVIPSIIGNLDQLVVLDLSGNKFSGPIPFAIGNLSRLNQLYLDGNDLSGNIPATLGNCKQLDMLKLSSNNLQGPIPSELINSTTLIRLDLSTNYLTGSIPPQIGALLQLFQLDISFNRLSGQVPLSLGQCVQLYSLRFRSNMLNGSIPQSFSNLKSINQIDLSQNSLVGQIPEFFANMGYLQQLDLSMNYFEGPIPTGGIFQNHSAVNLEGNDELCASATTTLYQFPVCTTTSAVGSKKNALLLVKVIPPIAIALLSLICFVITLLKRRQAQAAPCYKETMKKVSYGDIIRATNWLSPVNKISSSRTGSIYIGRFEFDTDLVAIKLFHLQENGARDSFVTECEVLRNTRHRNLVKAVTVCSTVDLENNEFKAIVFDFMANGSLDMWVHPKLHNNSPKRVLSLGQRLRIAMDVALALDYMHNQLTPPLIHCDLKPGNVLLDYDMTARVGDFGSARFLSSVPGSPEDLVGVEGTIGYVAPEYCMGYKVSTGCDVYGFGVLLLEMLTGRRPTDAMFTDGLSLHKLVSSAFPGRLGEVLDPHLSHEQQHACGRVFMRRYMVHLVEVALLCSMESPKDRPGMGEVCARILSIKEAFFEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WPL7</accession>
    <name>A0A8I6WPL7_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Triticodae</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WPL7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172280.1">
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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            <begin position="295"/>
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        <source>
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    </evidence>
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            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172280.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="547" mass="59811" checksum="88AF8985E33070CB" modified="2022-05-25" version="1">MQEWTNWFSPVSNGNPTEEEEGSQEGSVESAEYVSDSGESEEETSEEEEEDEEPDSPPPQTEHRTKRRHEPAVPSAPPASSSAPPTAPVVPSARSTKRARDAAVEPAGQSSKAAKPSGSKPRKALPRMRVAVPVTSTVATSATSPARQEDDLMDTDNVVSSQPGAICVDEGDQGRTEQAAEPVLEVAPPVAALAADVPPTEALSPTEPVLVVEVPTGTDLGMPQELPTMPGSSNVEFIIQRLPEEQVGAAKGAMVQAELMAGEAKRAYDSVASLYQQSLELRDDIRKTCEMGTSYETLKAERNQLAGELDAAMLAMAGMKDALEGREKSLEQAREANKVLTEEVEKMGKQRTALMGQMDVLNKRCRAQEKYVSDWARQMMTRLADFCFDAEAKAADVERSILENVPLSEDANRDLLRAHIRVGKVGPFIGRLREVVDRINKELSPEDESRQEMENLMTRLEEIPNRVQSWKKSAARCGADVALSLVRVHCKEVREEKMKTLKVANTKKLQFEDFMETFLESATRIADGIDLHTFVEPSSPGVNPDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSC2</accession>
    <name>A0A8I6WSC2_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">FAS1 domain-containing protein</fullName>
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    <organism evidence="5 6">
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        <lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958639.1">
        <property type="nucleotide sequence ID" value="XM_045102704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSC2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0045220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0045220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0045220"/>
    </dbReference>
    <dbReference type="GeneID" id="123409830"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0035110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0035110.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0035110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0045220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0045220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0045220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409830"/>
    <dbReference type="OrthoDB" id="765989at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052806">
        <property type="entry name" value="Fasciclin-like_AGP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33985:SF16">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33985">
        <property type="entry name" value="OS02G0491300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035207197" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="359"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0045220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="689" mass="74731" checksum="0D9275E451F23E5A" modified="2022-05-25" version="1">MALRLHHLPLLLLLTALLPTASATHSPESATSYSHHHYPTGTATAHFHPVHGAAPSMHQNHLETESQPLLAVEPAVADAQTTARPPLLVPVPPQAAAPSPPPTSVARPDLAASRSEATPQPREGSATATATTLPNQSTLPSPPPPVQAGAAAGEVVVSDSEQGLHQLSRALASLGYQEMAAAAGALLVNSPLFAKWPGPMTVFAAPDAFLQASCPTCSRRDLLIHHIAMGYYPYSELAAAAMVKIPSASVNFCINIVSEGGPFDTGLARIYAEDVEVSHPDLYNDGLYVVHGLVGFLRPLTHSCFDGPHPHHRRSLAGRSSGGTLVREAIARLRRRGFGVVAIALRLQFTELQRFANLTVFALEDQAIFARGGHDYVSSVRFHIVLKHRLTRAELLRLRPGTILPTLAGEDQSLVITHGAGENVFVNYIHIKESDVVVNSRIAVHGIESPLLHLNGELHSGGAAEPNLKKRFTLHDSQRPAEQGWARDRGVPLYWAVVGAAVAAMAGVAGAGSWFRWVKGKDGKRIYLAQAAMEATYNAKYGRRDLMAELEEQKKRLCAICSDEAFDWTATCCDVKFCCLCLGTAYVKRHNALYAPGPCVREGSLLPSLRVKLHPDLPGMYMESETRVKAEFEKALDQKDVHDEFVMKRVYTSHEDVLAKMAPQRILVYSREGNSEIIHQLNLGRTNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTR3</accession>
    <name>A0A8I6WTR3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar iron transporter</fullName>
            <shortName evidence="10">VIT</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vacuolar Fe(2+) uptake transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:28487"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The cytoplasmic metal binding domain (MBD) is located between transmembrane 2 (TM2) and transmembrane 3 (TM3).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTR3"/>
    <dbReference type="SMR" id="A0A8I6WTR3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0143540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0143540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0143540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0173670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173670"/>
    </dbReference>
    <dbReference type="OMA" id="MNFHHTL"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140315">
        <property type="term" value="F:iron ion sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02435">
        <property type="entry name" value="CCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="246" mass="26135" checksum="060B81EC56B41AE3" modified="2022-05-25" version="1">MDGHGDGRQPMLGKGPPSHRERHFTAGEVVRDVIMGVSDGLTVPFALAAGLSGASAPSSLVLTAGLAEVAAGAISMGLGGYLAAQSEADHYKREMKREQEEIIAVPDTEAAEIGDIMAEYGLEPHEYGPVVEGLRRNPQAWLEFMMRFELGLEKPDPRRALQSALTIALSYIIGGLVPLLPYMFISTVQDAMLTSVGVTLLALLFFGYIKGRFTGNRPFLSAVQTTIIGAVASAAAYGMAKAVQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTY8</accession>
    <name>A0A8I6WTY8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123427035</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M76 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044966902.1">
        <property type="nucleotide sequence ID" value="XM_045110967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTY8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176280"/>
    </dbReference>
    <dbReference type="GeneID" id="123427035"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0145770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0145770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0145770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0176280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427035"/>
    <dbReference type="OMA" id="EAHQNCV"/>
    <dbReference type="OrthoDB" id="285308at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="22532" checksum="BEE13069B51296D3" modified="2022-05-25" version="1">MAAAAGDGGSIPRKTTGAETPSASDPPVPRNIMPHEECVAGVRSALKDPMVRFLRERMEKAGCTVWPMLIRAATCASAGGYASREGIEVCCNHMEYPDQITQTVIHELIHAYDDCVGKNIDWTNCAHHACSEIRANHLSGNCHYKRELMKGFLKIRGHEPECVKRRSLESVKNNPHCSEAAAKDAIEAVWNICYNDTRPFDRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTZ1</accession>
    <name>A0A8I6WTZ1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA (cytosine-5)-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123426213</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10 11">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044965936.1">
        <property type="nucleotide sequence ID" value="XM_045110001.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WTZ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0151710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0151710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0151710"/>
    </dbReference>
    <dbReference type="GeneID" id="123426213"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0124800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0124800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0124800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0151710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0151710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0151710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426213"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071514">
        <property type="term" value="P:genomic imprinting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04708">
        <property type="entry name" value="BAH_plantDCM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000009">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000013">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000108">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000128">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000002">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000004">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022702">
        <property type="entry name" value="Cytosine_MeTrfase1_RFD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12047">
        <property type="entry name" value="DNMT1-RFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037404">
        <property type="entry name" value="DNMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BAH" evidence="14">
        <location>
            <begin position="741"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="14">
        <location>
            <begin position="910"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="672"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="700"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="1198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU000417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0151710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
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            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WV85</accession>
    <name>A0A8I6WV85_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123428453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968600.1">
        <property type="nucleotide sequence ID" value="XM_045112665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WV85"/>
    <dbReference type="SMR" id="A0A8I6WV85"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213520"/>
    </dbReference>
    <dbReference type="GeneID" id="123428453"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0177550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0177550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0177550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213520"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="266020at2759"/>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WVS8</accession>
    <name>A0A8I6WVS8_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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            <begin position="13"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="400" mass="44582" checksum="F4CC280632F391FF" modified="2022-05-25" version="1">MASMSSNLRRRPSLAPAPRSGAPDWRDWAGIHKSMSRRIAGHALANDVADYLQFRATCAPWREHTDEDDPKQHSVLDRRFHPRRWILMPNGSSTYVADDDSRNRHSMVNLSRRTKITVDLPHLQGHTIAIGCGGSPGGVLVLVHVDTLVVRLLNPLTGQRVDLASVAPVLSGHRMGHRDKEFSRKCKVLGAGFAGDSTVVLHFGWDNKLVSSNLGGDHWEIISQGDLYGSVFPFRGKVYFANQEREGLMVVDAEATPPNLVLAARWPTEGFNFRFVYMADSSGVLLVLTNKVDYQRGQRVDDDYTVEFNLFQLDAEAGVLIRVKDLGKRAMFVGDYSGALVVSARGKVVRNAIYFQHDLHEKLLVRGVSDGITRPMTEAEGGFIDDLTTYVRWNGSKREC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WWG0</accession>
    <name>A0A8I6WWG0_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC123424949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WWG0"/>
    <dbReference type="SMR" id="A0A8I6WWG0"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0101170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF37">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="1">
        <location>
            <begin position="770"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="934" mass="107033" checksum="5360826EB00D4133" modified="2022-05-25" version="1">MAIFDQCDTEDDFNGINEDSRGVISDIRREIFEKLVNSRFVVIFQNGSRKYIDFYELGVPAIPFLRNKVLWTWRGRFRLGLMRVTTDDEELEKAIMQNNVVLSCSLEREDEELQDLVVREEAEEVAKSTRFAEPGYLNHKIVWQCVMYGWLLRRITSATCLYFDTHGPNYWVCDGIIQGQGNTSAWEIGNSLQRNMHLDWIRKDPVKAIYLADSLKRNNDLDDDRWVYVTHKEPRWGDTVLAPMVTSFFLYADESSTNTIPMLPVSIFQHLQNSKLRVLHLSRCTFSFASPPFLCCSQLRFLQLDHCTSITDDDEYQSRNENIPCFQNLWVLDLRYTEWYWLLSEKMKRLMVDLRELNVEGVKHGGISDLCGGRPSLVILRVTAGPVHAENEDTNNQAQFPNMSSTNSVTTSSYINGIMPPWLESFSFINKAATPAEISSISFRGCSRLKSILFKGNLVSLQELDLSGTAVKTLDLRKVKAINLRCLILMGCKDLRAILWSSDNGWAILLEKLHISTVQSASPSPNHWEGITKEASAAAGSSSILAFGASPQGIGRTAYFEFQWYVSLVDPRLMWSLYRFQPHIITNFVYMEIDSSSASGATVFGREVTQGIGSQQHVAQYLYARDVFQEHSQVVSAIEGVISWMWACPPIPTPSPRDWYFHMQDEEKLERGSLRQQHNTPVHLAPVFMCDNARMLYVHDSSSTTCIPCPPDSDWNCIEWCRVERCPNLCSVFATPQLEDDGMTFSSLSTFWASQLPKACYIWNWSLIYQPDEQSFKYLKFLHVDYCPRLIYVLPLSKYMTTLPGLETLEIVCCGDLKVVFPLDAERQETQEIIKFPKLRKIHLYQLSTLQSICGSRMSAPELQTVKIRGCWGIRRLPAVSGSTTKRPMVDCEKDLWENLEWDGVEANHDPSLYEPGHSRYFKKDHLPRGTVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXM1</accession>
    <name>A0A8I6WXM1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXM1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0077940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0077940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0077940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0063330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0063330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0063330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0077940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0077940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0077940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33526:SF14">
        <property type="entry name" value="OS05G0512100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33526">
        <property type="entry name" value="OS07G0123800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0077940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="17399" checksum="24F0B4758A3C2295" modified="2022-05-25" version="1">MKQEKGQGGGGGGRKLGRWLGAPVRALSRACDSYVRKMSACAGAMPTQYAGAMGRGGAGAMHMQAATFSSRSTRRGDDDVGELVRAMSQRQQGSAASPAAASVPPRSRSVAVGRIDEDAPCDFGADAGRVGLPPPPPVRRSRSAAVGSGLPPRVGGGGGFGAATIKKAAVVHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXQ0</accession>
    <name>A0A8I6WXQ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RuvB-like helicase</fullName>
            <ecNumber evidence="8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123449672</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044982900.1">
        <property type="nucleotide sequence ID" value="XM_045126965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXQ0"/>
    <dbReference type="SMR" id="A0A8I6WXQ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085240"/>
    </dbReference>
    <dbReference type="GeneID" id="123449672"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0070120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0070120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0070120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0085240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449672"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="62"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="455" mass="49697" checksum="18F69477DD25692F" modified="2022-05-25" version="1">MRIDEVQSTSKKQRIATHTHIKGLGLDANGTAIGMSAGFVGQAEAREASGLVVDMIRQKKMAGRALLLAGPPATGKTALALGISQELGSKVPFCPMVGSEVYSSEVKKTEVLMENFRRAIGLRIKENKEVYEGEVTELSPEESESSTGGYGKSISHVVIGLKTVKGTKQLKLDPTIYDALIKEKVAVGDVIYIEANSGAVKRVGRCDAFATEYDLEAEEYVPIPKGEVHKKKEIVQDVTLHDLDAANAQPQGGQDILSLMGQMMKPRKTEITEKLRQEINKVVNRYIDEGIAELVPGVLFIDEVHMLDIECFSYLNRALESSLSPIVILATNRGICTVRGTDMTSPHGIPVDLLDRLVIVRTQIYGPIEMIQILAIRAQVEEIEIDEDSLAFLGEVGQQTSLRHAIQLLSPASVVAKANGREKICKADLEDVRGLYLDAKTSANLLHHQQGKYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X154</accession>
    <name>A0A8I6X154_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxidase</fullName>
            <ecNumber evidence="19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123431307</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in defense response to powdery meldew fungus.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="19"/>
    <dbReference type="RefSeq" id="XP_044971064.1">
        <property type="nucleotide sequence ID" value="XM_045115129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X154"/>
    <dbReference type="SMR" id="A0A8I6X154"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215410"/>
    </dbReference>
    <dbReference type="GeneID" id="123431307"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0179050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0179050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0179050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0215410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431307"/>
    <dbReference type="OMA" id="AGICSDP"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000009">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF51">
        <property type="entry name" value="PEROXIDASE 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 19" id="KW-0349">Heme</keyword>
    <keyword evidence="12 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="5 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035341611" description="Peroxidase" evidence="19">
        <location>
            <begin position="26"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="53"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="16">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
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    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="63"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="96"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1G2</accession>
    <name>A0A8I6X1G2_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123442352</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="hvg:123442352"/>
    <dbReference type="OrthoDB" id="624649at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="OSJNBa0016N04.16-like protein"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF76">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="550"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="593"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="768" mass="86718" checksum="290650A89B7D484F" modified="2022-05-25" version="1">MADGPRRVPFHSLEIMTDNFSPHLVIGHGSYGKVYLGKTEDGQKIAVKILHDMSGHDDVQFEKEFYNLVNLKHQNIVRLVGYCHETRREYLSCNGAMVFADAIKKALCFEFMENGSLKDCLFDEFNGHGWRMRYAMIKGICNGLKYLHEEVEPPIFHLDLKPANILLDQNMIPKIADFGLSRFFKEEKSHSTNSPIGTRGYLPPEFIHHRIISNKLDIFSLGVVVIKIIAGPEGYSRSAETTSDQFTKTVHDNWRDRLQSTASGDLLESYSEQVKICIKIALSCLEADRRKRPTIGDIVNILTETETLDKMSTCMVDDTNLIDVHPLQLHFSFRPNKCISCLFHVTNNMNDQHVLVRCVPKHEEATPRYYACRSRITGVVPPRSTHTFVVTMVEQEQPPAITDVLDMVVETILGEVKLPSLGDLYDTHFENARKENSCGFREVTLKAVCATTSKTTTSEVLIGGDGLRSMDVHPTEPWILAGYFRRLVIWNYKTQTKMMEHSLISVISPIAFVSTVKFIIKSTEQLFVVGDGDGYVHVHDSMTMKILKKFKAHGRRVRSFAVHPTRPFVLSASWDKLIKLWDYENGWSCIRTFTGHSGPVTRVKFNPHDANTFATASSDRTVKIWSISSPIHVTSLNCEDEQACVDYYHTTATVTGSYSHGTARIWDLRTQTCIREIKGLQSPCQVGVIDCHPESDRPTLLITASEDNGVSLVDSTTYRSERTIHFGLGGVLCFAYIKGVRSVAIGHEHGIAILEIDRFFPKVLPPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2J3</accession>
    <name>A0A8I6X2J3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123410055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958877.1">
        <property type="nucleotide sequence ID" value="XM_045102942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2J3"/>
    <dbReference type="SMR" id="A0A8I6X2J3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0046700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0046700.1.CDS1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123410055"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0036370.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0036370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0046700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0046700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0046700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410055"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
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        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RlpA-like_sf"/>
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        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF80">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035312674" evidence="5">
        <location>
            <begin position="24"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0046700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="144" mass="14925" checksum="4BA4AF5C0DB75C5A" modified="2022-05-25" version="1">MAYGKLAALAVLVVLLQASTGAASRSSPAVSASKIDSMPAEMSLNGFERGEDGGAPAECDGKYHSDGDMLAALSTEWYAGGARCFRTIRVTNSATGQSVEATVVDECDTRRGCKSNIVDTSKAVWKALGLDTNVGEVAVTWSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3S1</accession>
    <name>A0A8I6X3S1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123439287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971956.1">
        <property type="nucleotide sequence ID" value="XM_045116021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3S1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227220"/>
    </dbReference>
    <dbReference type="GeneID" id="123439287"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0227220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439287"/>
    <dbReference type="OrthoDB" id="2015495at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035301044" description="CASP-like protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10511" checksum="51B27FBB12429B6A" modified="2022-05-25" version="1">MASIIVQVSALLLNLFAFGLAVAAEQHRSKATVTPDLAKEYDYCVYDSDVATGYGVGALLLLTAAQVVVMLASRCFCCGRGLKPGGSRACALMLFLFSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X519</accession>
    <name>A0A8I6X519_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965649.1">
        <property type="nucleotide sequence ID" value="XM_045109714.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965650.1">
        <property type="nucleotide sequence ID" value="XM_045109715.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965651.1">
        <property type="nucleotide sequence ID" value="XM_045109716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X519"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142550"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142550.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142550.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142550"/>
    </dbReference>
    <dbReference type="GeneID" id="123425945"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0117410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0117410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0117410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0142550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0142550.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142550.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF69">
        <property type="entry name" value="OS07G0507600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="2">
        <location>
            <begin position="60"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="618" mass="70158" checksum="98FF040AFC7BE0E5" modified="2022-05-25" version="1">MNPTAHDQTTQQQKQNGRRAVGSSQTWTRYGVLLPSQQTRKLKEWVLTDEQQQVVNASGLGHLALTTGFTIDRSLLTAFCERWNNETNTAHFMGFEMAPSLRDVSYILGIPVTGHVVTAEPIGDEAVNRMCLHYLGQSPGSGEQLCGLIRLTWLYRKFHQLPENPTINDIAFSTRAYLLYLVGSTLFPDTMRGFVSPRYLPLLGDFRRIHEYAWGAAALAHLYRGLSVAVTPNATTQFLGSATLLMAWIYEYIPLTQPQQKNQNTLLPRACRWNFGGTTRGQRKKVVEWRKDFEQLQLSDVNWNPYKDINPAIVPGYCIAADNICYSRTWLISFNIKEVYVPDRFNRQFGREQGRLHQVPMWARRTWSKGKDWRVEYAREIEDFHQLVGCRFIPSMEANINSLPNESVAGQSTARCSRNASQNFSMMVEDLKNDLPVIDRYLEGQVLPVEVASFLERVSTMIKTYSPSQSNARKDEAAQSKNVRAKNPRKRARKPLISEDPSSPQDSGVDRYAGALVPYQASKCDRVVDGTVPLLNRGEVLKENEVMDPWQMSHLTAESSSSGDSGFPESRRWRRRREESGTPRSNAGVRRSGRMCGQLKMLKHRDGVGAEETNEILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X764</accession>
    <name>A0A8I6X764_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123419879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probable phosphatase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CTDSPL2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X764"/>
    <dbReference type="SMR" id="A0A8I6X764"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0073150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0073150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0073150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0088930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000015">
        <property type="entry name" value="CTD small phosphatase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="5">
        <location>
            <begin position="211"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="385" mass="42063" checksum="2A40BF47E825B1AE" modified="2022-05-25" version="1">MANQVPYEVMEIMEDADAVGEDCWDLLEDLTPATTSSPHFQASSEFNVEDYVNEGGDGILAAPSFFSNGDGILAAPNRTIVNSNEITQDSGQSCSLLSSFPTEDSPLYMLDSSDGVLSNSYAMDDLTTMSGSYGVLPSSHENVRASSIAFAGTTEDLCFDNNQQSQFLGQPSTHSSPEIIDLTEMGGMDETDGVPMEALTPELVDDVLMSPLRKNVTLALDLDGTLIHSARCDHGADFSFPMRRGEQEYTVYVKKRPHLDAFLEEAAHMFELVVFTASISSYANQLINALDPENKLISRRFFRESCVSVDGSYQKDLTIIEADLAKVAIVDNTPEVFQQQVNNGIPIKSWSSDTSDISLLELIPFLETIADADDVRPIIANKFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8G9</accession>
    <name>A0A8I6X8G9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27485" checksum="1541077C2B69A9AF" modified="2022-05-25" version="1">MALATYLLVMIVVQNQMELSHPALVVSATALMLILLLPLAVVVKQEYRIKRELEDSLLVPSTVAVEKPPAAAPLQMAAKAETEEAPATKAEDAATPASGGCFGSCLKEMFSPPAQGEDYTILQALIWNYAGRVTAGFVSEAVLARYKFPRPLMLTLVLLLACVGHLHIAFGVRQSLYAASVVIGFCFGAQWPLLLAIISEVFGLKYYSTLYNFGSVASPIGAYALNVRVAGYLYDVEAARQHGGTLDGVGDKTCWL</sequence>
</entry>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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            <dbReference type="Pfam" id="PF18517"/>
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    </evidence>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="PANTHER" id="PTHR33416">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416:SF16">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="909"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1133"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0449530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1142" mass="120196" checksum="9448B9E22D2B2369" modified="2022-05-25" version="1">MANPQIPLFLGSNLLALCNHHFTLSSLRFPILFSKTLAPHRRRNMASRGGYEGGSATGGKIRRRPPFRAAAASPYARPAAPIHPASRCSEGSGWLARLISGGASRLLSSVFRKPAPQLAAPTLWEPALVDAPYSPPPPPLENDILEEENGGGTANNLSTDNPENSAIDGDDCLSSCCNGSMDLEELLKQKTFTRSEFEYLTGLLRSRTIGSNTVQSEINNIKQTLSPKKEKGSRDLPVDFSIRSYNVADQVASPAELAKAYMGSRCSEGPPLRLRLHKPSSLSVKSIESGTIQIAKSPKAPLLGSSRLSASTPFDRLGSNYRTPNKSAIHKMSSSPYFKAPVSSTDICGTVSSSYQTANSIHTFGRQLLKRKNIALNNETDSVGPIRKMHQRYNRVSSLSETWPGYREYPGSHATKLDEDFEHSTQTQKRLCLAEVDNGTLGICGNNFVQAPAQSTEMAAKILKQLDTLVSSPSEKESSPEMKQKLGNALDVEDSISQRKEISSQGSLLESSSSFIQEYSLLKNRNVAAIFAPTAIDVKTVDAMSNGSALPGSKSSSELITSPKDSLELDHCSGSIEIEKSYSPVQEHTANNSGTTNKENPPAVSLRSYSPSNLMLSSEIKQTKMLSSSNGFSFPVMAPPVAHSQAPPTPTMASPPALHVGKHKSSALPSVPVTSVENAPWILRQVSEESSVSDKHNKKLNGEIPPISSKGAGHVASFTSNPVTLSNGLAHTSKSTASAVLASDGSNNSFLSTNTVSSTSLLPPFSSQPGLETSFTSAQKFGGIQFKAEPIITLPFSMHSNSTGGSFTFSNTGPSSSSTSSKTFASITSQLAKAPTSGSANAPYNFSPNFGGASLLAAQDKSKVGSSTAALNFSPHFGSVNLVASLDKSKVTSPESMLLAGNQFARPGNSNSLCTQSSATKSDLMSPDKSNMGSLPFGSAPLSPSTFSSSSVVSSTAASGSASVTTTTPPPVPFMASSALGSSKAFSVSPIFGSNPITTAPSSFGMLNNGSAMPFSPSSTVFSFTSATHIIPDPPPSTPLFGSTIPTFGFSTGADQMNGGQTTVGNNQTPFATASPFGFQSNSLSTQAFSMPASQFASTSTTSPGIFQFGQQSQASSGVFSVGTVRDNDKSGRRIVKVKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XC48</accession>
    <name>A0A8I6XC48_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XC48"/>
    <dbReference type="SMR" id="A0A8I6XC48"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0372360.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0372360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15624" checksum="9A078461569C6D82" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDVEVCEPEIPTHIKILVEKLNLVEKENKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIGNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XC80</accession>
    <name>A0A8I6XC80_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338710"/>
    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="420" mass="47685" checksum="0E8AE507F1FBE268" modified="2022-05-25" version="1">MVVVLRFVNIEGSIRERFLDLVHVSDTCALTLKNKIIAALVDNGLNVQDLRGQGYDGASNMRGEWNGLKALILQECPYAYYIHCLAHQLQLALVVNSREVNEVHNFFQHANFVVNTVSASPKRNDELLENQAAEIAREIELGELDTGRGANQIGSLQRVGDRRWSSHYKPIKSLLKMFAIAVSVLRSVATDRSATRNSRGDPAGALKILNTFDFVFILHLMERIMKITDIFCVKLQNKSLDIANALDCVSNTKSLLAELREDGWESLFEDVKSFCVKHEIDIPDMDQRYVDVTKSRNKHDNTTVIHHYKVDVFNVAIDQQVIELNDRLSSQVTELLDLCSSLDPRHDAFDKSKICTLVEKLYPADFSSQERDRLECELPHFQLDTFNNPEIKNCKSIADMTKEIIKSGKSSDYPMVERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCX1</accession>
    <name>A0A8I6XCX1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCX1"/>
    <dbReference type="SMR" id="A0A8I6XCX1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0094050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0094050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0094050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0114430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="109" mass="13324" checksum="F25C7FB9D3BBE755" modified="2022-05-25" version="1">MPDWAVTEIRERGHRAWASLDLEECHAKAEAEEKAEQKKEWEDYCVEQRAFIDEMIRKNQEEKLWLEEVYRQREQAREAERERKRERARAAKAAEEAGDGKWKYQRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XE18</accession>
    <name>A0A8I6XE18_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="139" mass="16410" checksum="7DEBFEBDAED84BFE" modified="2022-05-25" version="1">MQFTGPTFRFPLTVLERLYPAEVYMERTLRVWAMSRRRGARELTNAFLALDFSHLPWGSPRMYRVEEVIHNGVVVAILAHFTNHIDAFYLLGRAFWCDCEFIAFTTHNIFTDYNNIFPTAERMHTLSYPIDNTPEEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIZ8</accession>
    <name>A0A8I6XIZ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIZ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0502090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0502090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0502090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0416880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0416880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0416880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0502090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0502090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0502090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175:SF70">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0502090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16933" checksum="A12FF5EBD44025E4" modified="2022-05-25" version="1">MNHPKKLAQLAKKCQRMLAVGAGACRRQHASDTADDECCSTTSSMVANEGHCMVYAADGERFEVPLAYLRTTVFVELLRISEEEFGFASGSDRGRIMLPCDATVMEYVLCLVRREASEEVERAFLSSIAEHCHNYSASCVAPSMGLSHQFALCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNT8</accession>
    <name>A0A8I6XNT8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442492</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974486.1">
        <property type="nucleotide sequence ID" value="XM_045118551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNT8"/>
    <dbReference type="SMR" id="A0A8I6XNT8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223990"/>
    </dbReference>
    <dbReference type="GeneID" id="123442492"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0185990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0185990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0185990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0223990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223990"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141131">
        <property type="term" value="F:DNA N6-methyladenine demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010029">
        <property type="term" value="P:regulation of seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009845">
        <property type="term" value="P:seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000020">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase alkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF11">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="250"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="370" mass="40525" checksum="A26523C3DB94DFFA" modified="2022-05-25" version="1">MYAETEPGAAAERTAFRRAEKQYKLYKPPSRKGRSRGKQSGGGGGGVGGGGGGGDLSAVVDFHALLAAGEGELPAGIRRCHRAGFHLPVFCFLDRPGFYFIPGALSTEEQCYWIRESLKTFPQPPNRTNLTAAYGPISDLLVAAKNQKILVEVESSNGQERDEQSNVGENAYPGKFKFVDGSEVQKEERPKSTTAAALVRKLRWSTLGLQFDWSKRNYDVSLPHNKIPDDLATLAKKMAVPAMPSGEEFTAEAAIVNYYGPSDMLGGHVDDMEADWTKPIVSISLGCKCIFLLGGKTRDEAPTPMFLRSGDIVLMAGEARERFHGVPRIFTESDQQGISALVSELSGDDDRFILDYIHNSRININIRQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XR40</accession>
    <name>A0A8I6XR40_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123449989</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
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    <dbReference type="RefSeq" id="XP_044983318.1">
        <property type="nucleotide sequence ID" value="XM_045127383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XR40"/>
    <dbReference type="SMR" id="A0A8I6XR40"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416120"/>
    </dbReference>
    <dbReference type="GeneID" id="123449989"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0346820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0346820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0346820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449989"/>
    <dbReference type="OrthoDB" id="633985at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47863:SF8">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47863">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="765"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="111"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="583"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="619"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="841" mass="92069" checksum="F757A1657E74446F" modified="2022-05-25" version="1">MAPAAAVDPGESTPATAATPRTGPARPDLARRLPVTPRELGFTPFRNPPPQEEGALQNSSEGKIQAPELKPLAGSQAVPKTEGVSTPISLVKSIHAQLDAQIPVNADLSQRKPPAKATMLPKRDPPPLEGALPNSSEEIQNPEWKPHARSQAPPRTVDVRTPVSWVKSAREALAHKAFTDEHNALLDAQILVNGDLSQGKPPAKSALRPVRNPPLSQGKPPAKCALRPVRNPPRLEGALPNSPEEIQTPEWKPYAGSQAVPDTMGVRTPGSSLKNTCEALAHEAFTDEQHAQLYAHILITADLKQGKPPSKAAMVLAFGQPVEGQKHAWEEVWRAALDRYQSQMSPLGFETPFSSQNGPLEPSPNSMKNLEEFEPKEAHSELVDAQTSKWQNQKSPVTGFETPIKSLIGNCTAQGLDVQNGGEVQLAVSCADKVDQEPPDDSKIKNRIYGGLESLCGSTPLESSESLTEQDSCVKCSKDGQLLKCSSCFLAAHASCFGSSVTFDDSGHFDCPVCYCRKAAEALEKAKKTYSEARKNLSVFCSGWKQFTKEFSEQSIGTQQRAANFEDQMNGFNTAKKQGNHQSEAAKLSCKDEEPNHLKENGTSDACPDKVVTTKTTVSPSSENAKSHGDGNSNSSRQVPYSARDKFSSVANHNIGVGKENCLMNSPNCNFSDRPGAMSSRNMSRRKVSFQEMGTVAPNSTWKTLRYQDQFVHSPARKRYYPYPPEHYYSPHTPAMRSPLAPTKTPFTGKTPFTSTTARRKMIFWSEAEEVALREAMAKLAPKGEAVKEKRSISWVRIHEYCRESIHPTRCPDDLRKKWNRMKNKLGVCPEDDDRVGDGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRP6</accession>
    <name>A0A8I6XRP6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123440414</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044972917.1">
        <property type="nucleotide sequence ID" value="XM_045116982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRP6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0296950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0296950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0296950"/>
    </dbReference>
    <dbReference type="GeneID" id="123440414"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0247320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0247320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0247320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0296950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0296950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0296950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440414"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="48"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0296950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="263" mass="29935" checksum="FD1D4821689CCCBF" modified="2022-05-25" version="1">MEWNFGPEEQVLWPASALAGVLMCAAVYDITRKVSSSCFKCYDGLSPMQKLEWNNRGFSTVHALVAAAVSFYLVMISGLFSEDVNDIIIDRKSWLSDSMFGVSIGYFFTDLAMILWYFPSLGGKEFLLHHGLSMYAICLALFSGKAHMYILMVLFTEATTPIVNLRWYLDAAGQKTSNLYLYNGLALFAGWLIARIILFVYFFTHIYFHYDQVKSIFALGFYTIMTVPSTLAVMNVFWFWKICRGLVRTLSKTKMHTANGKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XST8</accession>
    <name>A0A8I6XST8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="5">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123452349</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044984925.1">
        <property type="nucleotide sequence ID" value="XM_045128990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XST8"/>
    <dbReference type="SMR" id="A0A8I6XST8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512380.1">
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    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="15">
        <location>
            <begin position="39"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="14">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047891"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="374" mass="40382" checksum="7B91EDAB346D2070" modified="2022-05-25" version="1">MQATAAVHRPHLLAASPLGARASRRPSTVRMALREDGPSVAIVGATGAVGQEFLRVITARDFPYRSLRLLASERSAGKRIEFEGQDYTVQDLAAPGAFEGIDIALFSAGGGVSRAHAPAAVASGAVVVDNSSAYRMDPDVPLVIPEVNPEAMADVRLGKGAIVANPNCSTIICLMAVTPLHRHAKVKRMVVSTYQAASGAGAAAMEELKLQTQEVLEGKPPTCNIFSQQYAFNIFSHNAPIVENGYNEEEMKMVKETRKIWNDKDVRVTATCIRVPTMRAHAESVNLQFEKPLDEDTAREILRAAPGVTISDDRAANRFPTPLEVSDKDDVSVGRIRQDLSQDDNRGLELFVCGDQIRKGAALNAVQIAEMLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XT92</accession>
    <name>A0A8I6XT92_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XT92"/>
    <dbReference type="SMR" id="A0A8I6XT92"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0574120.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HHO5-like"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom"/>
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        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
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        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003:SF45">
        <property type="entry name" value="MYB-LIKE DNA-BINDING DOMAIN, SHAQKYF CLASS FAMILY PROTEIN, EXPRESSED"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="117" mass="12693" checksum="523DE860DFAE6FCB" modified="2022-05-25" version="1">MPLNGLPNLVKSSERPEAAAMAVPELSLSSPAIDAPYMAAPSADSSAVTDARAQSQQQSGQRKARRCWLPELHRRFVAALQHLGGPQVATPKQIREMMKVDGLTNDEVKSHLQVCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTA4</accession>
    <name>A0A8I6XTA4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123449535</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044982723.1">
        <property type="nucleotide sequence ID" value="XM_045126788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTA4"/>
    <dbReference type="SMR" id="A0A8I6XTA4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402920"/>
    </dbReference>
    <dbReference type="GeneID" id="123449535"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0335390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0335390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0335390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0402920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000002">
        <property type="entry name" value="Prolyl 4-hydroxylase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF219">
        <property type="entry name" value="OS03G0166100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00254">
        <property type="entry name" value="ShKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237212" description="procollagen-proline 4-dioxygenase" evidence="15">
        <location>
            <begin position="26"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="122"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="306" mass="33208" checksum="A6A415F8B6E1412F" modified="2022-05-25" version="1">MAPLLQRVLLALAVLLLASTASSSATASGGVHFDPTRAVHVSWRPRAFLYKGFLTEAECDHLVALAEEGGLQKSMVVDRQTGKSVMSEVRTSSGTFLAKKQDQVVATIEARIAAWTLLPQENGESIQVLRYENGQKYEPHVDFIRHAAKGHHSRGGHRVATVLMYLSDVKMGGETVFPNSDAKTLQPKDDTQSECARRGYAVKPVKGDAVLFFSLHPNGTTDPDSLHGSCPVIEGEKWSATKWIHVRPFDNRRRVPSTAGCGDDDELCPRLAANGECARNPRYMVGTAGSPGFCRKSCNACTGTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUN9</accession>
    <name>A0A8I6XUN9_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6XUN9"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15860" checksum="582ABC0E7BC15260" modified="2022-05-25" version="1">MVLVSAPVGPRLVYDSDVEVCEQEIPTHLEMLLEKLTLVEKENKYLKEKLKIIEDKNMKMELHVAEVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYVVGSIVTLLAVLIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUP2</accession>
    <name>A0A8I6XUP2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enolase-phosphatase E1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUP2"/>
    <dbReference type="SMR" id="A0A8I6XUP2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.60:FF:000001">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000088">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00044">
        <property type="entry name" value="enolase-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26593" checksum="1B9B34AE15F44588" modified="2022-05-25" version="1">MDIEGTTTPISFVADVLFPYARANARAHLAATYHTKQTQEDIALLHAQILQDLADGVPGAVPLPPPDDTDQDKTIDALVANVQAMINADRKLTALKQLQGHVWRRGFESGEIKGEVYDDVVQALAEWDARGIKSYIYSSGSREAQRLIFGNTVAHGDLRRYLSAFFDTNVGGKKESRSYYEIWQTLGVDSPSQILFLTDVYQEATAARDAGLEVLISIRPGNAPLPEDHGFQTITSFAQIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWZ6</accession>
    <name>A0A8I6XWZ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWZ6"/>
    <dbReference type="SMR" id="A0A8I6XWZ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0340720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0340720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0340720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0340720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0340720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0340720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0340720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="223" mass="24726" checksum="BEA2672C674604F7" modified="2022-05-25" version="1">MYDLYAMAHTASYKKVKAFCESDLEHPENFTNISSHHKLVKYRDHGKARKGEDFNPSEGPIDLELVMIAGKGRPHGLISIGYGLIHCPSTLRQIKARQTSSCPEITRRPRPGELAIEAAIQKEREAMQAAMAEKDKEIRELEERTTALVEAERARNDASNRAIYELFVSMCEKNDQVPPPMPVINVAGTNISRNASHDPSTVEPSPGQPSPGETSQSHRASPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYS3</accession>
    <name>A0A8I6XYS3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase 61 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123403069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044952977.1">
        <property type="nucleotide sequence ID" value="XM_045097042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYS3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553290"/>
    </dbReference>
    <dbReference type="GeneID" id="123403069"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0459650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0459650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0459650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0553290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403069"/>
    <dbReference type="OrthoDB" id="529273at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="8">
        <location>
            <begin position="330"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="504" mass="55775" checksum="51246BAFB919CB81" modified="2022-05-25" version="1">MNMSCSEERERPRRSWAPVVHVNFGFVAGVVFVLLVYFVVQQQEAISALGVATTTVVSQWMSHKQLIKAPREIQPIKAPGEILVASDVHAIAGKQPTQDTVLASDVQPIVDKQPIHDSENGEVVCHTEGLSRGYSETCEVDGDVRTNGTALSVTLVPASRSVRREWMIMPYAKMEVVVRNVTVTQLQDRAAAPPCTVTHTMPAVLFAIGGYVGNYWHDYTDILVPLFVASRRYRGEVTFLISNIQHKPEWLVKYKTLLGALSKHAWVDMDGDTEVRCFPHVTVGLRLDKELSIVPELVPGAPLSMVDFTRFLRETYGLPRGAAVSLTREPDKKPRLLLIHRGHYRLFLNELEIAQAAEAAGFEAVVMELRGNASEVEQARLVNSFDVLLGMHGAGLTNALHLPPGGVLIQVVPYGNIEVIARSEFSEGATDMGLKYLDYSVSAEESSLMELLGPEHPAIKDPDSIHRSGWTNMFDLYLGKQNVRINTTRFAPTLAQALGHLRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3E0</accession>
    <name>A0A8I6Y3E0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123449448</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982615.1">
        <property type="nucleotide sequence ID" value="XM_045126680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3E0"/>
    <dbReference type="SMR" id="A0A8I6Y3E0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400070"/>
    </dbReference>
    <dbReference type="GeneID" id="123449448"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0332910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0332910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0332910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0400070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449448"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="241"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00057191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="489" mass="55086" checksum="BF0582EB5DEAE726" modified="2022-05-25" version="1">MTEDVQMGEPQSAPPPAAAAAPAPSTLQHLKEIAAVIEAGSLSKEVRRISRAVRLTVALRRRLAARDVSAFLAFALPASSDAYARLTPLLPKEDEAEMDVDAAAPPTQISIKHGLPEIEIYCSLLVLIFLIDQKKYDEAKSCATGSIARLKNLNRRTVDVLASRLYFYYSYVYELTNTLAEIRGNLLGLHRMATLHHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPAAARGFRIQCNKWAIIVRLLLGEIPERTVFMQKGMKEALTPYFELTNAVRIGDLELFRAVADKFASTFSADRTHNLIVRLRHNVIRTGLRNISISYSRISLADIAKKLRLDTGNPVADAECIVAKAIRDGAIDATIDHANGWMVSKETGDVYSTNEPQAAFNSRIAFCLNMHNEAVKAMRFPPNSHKEKESAEKRRERLQQEEELAKHMAEEDDDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4Z6</accession>
    <name>A0A8I6Y4Z6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="4">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123402682</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRB family specifically reduces the MetSO R-enantiomer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044952558.1">
        <property type="nucleotide sequence ID" value="XM_045096623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4Z6"/>
    <dbReference type="SMR" id="A0A8I6Y4Z6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0619510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0619510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0619510"/>
    </dbReference>
    <dbReference type="GeneID" id="123402682"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0513860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0513860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0513860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0619510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0619510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0619510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402682"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF4">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="5">
        <location>
            <begin position="11"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0619510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="133" mass="14405" checksum="4C78BC97276E4317" modified="2022-05-25" version="1">MASGDSKPRSEEEWRAVLSPEQFRILRLKGTELPGTGEYNKFYGGGVYNCAGCGTPLYKSTTKFDSGCGWPAFFEGLPGAIHRTPDPDGRRVEITCASCGGHLGHVFKGEGFKTPTDERHCVNSVSIKFTPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y508</accession>
    <name>A0A8I6Y508_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123447747</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980334.1">
        <property type="nucleotide sequence ID" value="XM_045124399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y508"/>
    <dbReference type="SMR" id="A0A8I6Y508"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341640"/>
    </dbReference>
    <dbReference type="GeneID" id="123447747"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0285140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0285140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0285140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0341640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341640"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADENYLYLSULFATASE HINT3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01230">
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        <property type="entry name" value="HISTRIAD"/>
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            <end position="168"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="199"/>
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    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="153"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="155"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="21645" checksum="6E2708A6C6DBCE19" modified="2022-05-25" version="1">MSPPRRLAVLCSHLRPDGPEPPAENERTAGQQTVISTSPCAAAVDDAADGRVGDRGVESCVFCRIIRDEAPAFKLYEDDVCICILDSHPLAPGHSLIIPKRHFSSMEATPPHVIAAMCSKVPFLSNAIMKATDCDAFNLVVNNGEAAGQVIFHTHFHIIPRRSGDKLWPTESFRRSSIEPNETSSLISGIKKQLDSSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6U3</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6U3"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0372550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="27878" checksum="0B79103788CDDA1D" modified="2022-05-25" version="1">MVGVPHHQGSANLLQFGRNLAHHNKTEMPHLYDLYGMAHTASYKKAKAFSESDLDDPNNFTNISSHQKLVAYRDAGKATKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKARQSSRCPEITSRPRPVELAIEAALQKERAANQAALEKERLASQAALQAPLDERDQTTARLLEEERARNEAGQRALYELFVGLCEKSGQVPPSMPVFSSIGMNNSRATSHDPSPGQPSPNEADASNRGVSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y842</accession>
    <name>A0A8I6Y842_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Peroxidase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123410244</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 19"/>
    <dbReference type="RefSeq" id="XP_044959091.1">
        <property type="nucleotide sequence ID" value="XM_045103156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y842"/>
    <dbReference type="SMR" id="A0A8I6Y842"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677150"/>
    </dbReference>
    <dbReference type="GeneID" id="123410244"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0561960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0561960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0561960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0677150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410244"/>
    <dbReference type="OMA" id="ESMCPRT"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        </location>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <ligand>
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        <location>
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        <location>
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        <ligand>
            <name>substrate</name>
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        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
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            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </location>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="41396" checksum="59A48EE200491A9B" modified="2022-05-25" version="1">MSMAMLFLLSLVLASSAPRLAVVEALVVDGLKMGFYNRTCPEAEQVIRNVVQTEVGMDRTIAPGLIRIFFHDCFITGCDASILLDESPSGDVPEKESSANGFTLHGLRTIDVAKSTIEAMCPRTVSCSDILSFAARDAAVAAGLPSYEVAGGRRDGVHSRMDDLPGNFPVPGHTVPRLTELFQSRGLSQEDLVTLSGAHSIGGAHCFMFSNRIYGFSKTSEIDPSLDPAYAERLRKICPRPRPDDDPEAAPKVDFDERTGQKLDNSYYQELLARRSLLTSDNTLAMDPQTRPLVEQYAKDDALFQKRFGEAMQKVSALDVIIQKTKGQIRRDCRMVNKARGIRWMPMLHFPKPPKHPMLQMINWFIRGFQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBI5</accession>
    <name>A0A8I6YBI5_HORVV</name>
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        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="14" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
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    <dbReference type="SMR" id="A0A8I6YBI5"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF46">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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            <begin position="211"/>
            <end position="232"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0563760.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="25788" checksum="B9561619BA2824A9" modified="2022-05-25" version="1">MLFLFNNELTGTIPVWISTLNFLFHIDLSNNGLMREIPAGLMEMPVLKSDTIKTSLDPKGSELPTYSGPSPRYYTLGAFPAMMNLGNNNLTGVIPPEIGQLKALVSLILGFNRLSGEIPRTISNLTNLEVLDLSNNHLTGTIPGALNNLDFLSRFNISNNDLEGSVPHGGQFDTFPSTSFDGNPKLCASILIHNCHSIEVPKGSTEQGYKVMFAIAFGVFFALGVLLDQLCLPRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCK8</accession>
    <name>A0A8I6YCK8_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123410788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044959662.1">
        <property type="nucleotide sequence ID" value="XM_045103727.1"/>
    </dbReference>
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        </location>
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        <location>
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            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="401" mass="40077" checksum="4037B8FBCB833924" modified="2022-05-25" version="1">MSRLAAAAAGALLVILAVVGSAAAKTTIEPCSTADSCAALLGYSLYADMKVSEVAALFGADPAALLAANALDFASPGAANRILPAGLLLRVPTRCACADGVRKSVSVRYAARPADTLATVADVVFAGLASADQIRNANGLAEADPDALLDAGQILVVPFPCVCLNSTDNNLPAVYLSYVVRVGDTVESIAASHATTVTDLSNVNAMGSPIVAPGDILAIPLSACASTFPNFASDYGLLVANGTYALTAGNCVECSCGPGDLNLYCTPASLGTSCSSMQCSNSSLMLGNMTSQPTSGGCGVSSCSYAGFINGSIATSLSSGLQPTCPGPHQFPPLMEPSTAAIHGSYLAPSPSPGPGEAGGAVPGSSDSGASSHSLAGSLSPSVHRLHQMILILSLVFYLHM</sequence>
</entry>
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            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
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    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="SMR" id="A0A8I6YG10"/>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="327" mass="36558" checksum="9BE25691E46BF9D3" modified="2022-05-25" version="1">MERMASSYFKEVYTKDPTLVANEVLQCIIPKVTLEMNEALCKPFSEQEISDALFQIGPLKAPGCDGLPPHFYQRNWSVLKPEITVAVQEFFNTGNMPEGVNDTSIVLIPKVPHPKELKDFQPISLCNVVYKIVLKCMVNILRPFLTELISEHQSAFIPGRLISDNSIIAFECIHHTQSMKENSPALCAYKLDLSKAYDRVDWDFLVMALMRWGFSQTWISRVMACVTSVKYSVKFNGKLLESFSPSRGLRQGDPLSPFLFLFVVDALSALISKSMREDGLQGVKICRGAPEISHLLFADDSLLLFHATGQQAVLVKGLLNTFASATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YH97</accession>
    <name>A0A8I6YH97_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YH97"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0720270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0720270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0720270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0720270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0720270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF78">
        <property type="entry name" value="OS06G0232700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="63"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="172"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="353" mass="40739" checksum="E4F55CF68DFD22D9" modified="2022-05-25" version="1">MAEMPQLLNGYHDNHHRCQLFINPNHENPPQTFRLRTVKTPWSIENRFLEHLEAYGLLHFANIAARRGSFTADPSLLTSLVDRWSPETHTFHFRCGELAPTLKDVSMITALPIRGVPVVHPRVSPNWPADVSARLRLEMPISDRSGPPRGVPHILPGLIYFAAKIVDEPLPQNPPYSFGSALLSHTYRGLCDATQKTTFTSKAPLLCVSYEFLQLWSWEYLPVGRPQIVEPATPYNYGEGVVTMSTRWMLGRKKWSTKIAKNCYPIYHDQFELLNDSLITWNPWTEAHIDMVFGSQHMPVECVRDSAFWMTRCNLLYLWFVEPYNPNRVMRQFGLYQDIPPPVPRCIDEETHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJN3</accession>
    <name>A0A8I6YJN3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJN3"/>
    <dbReference type="SMR" id="A0A8I6YJN3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0546050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0546050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0546050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01806">
        <property type="entry name" value="Ubl_NEDD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000023">
        <property type="entry name" value="NEDD8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038738">
        <property type="entry name" value="Nedd8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="17158" checksum="A217C0A8740A468D" modified="2022-05-25" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLTDYNVQKESTLHLVLRLRGGLSIKVKTLTGKEIQIDIEPTDTVDRIKERVEEKEGIPPIQQRLIFGGKQIADDKTAHDYKIKAGDVLHLVLALRGGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLM7</accession>
    <name>A0A8I6YLM7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0610690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0610690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0610690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25842" checksum="68A072538B56DA2C" modified="2022-05-25" version="1">MSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEEPIHWSYGLGVNVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YM51</accession>
    <name>A0A8I6YM51_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123411079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044959934.1">
        <property type="nucleotide sequence ID" value="XM_045103999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YM51"/>
    <dbReference type="SMR" id="A0A8I6YM51"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0732120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0732120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0732120"/>
    </dbReference>
    <dbReference type="GeneID" id="123411079"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0607560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0607560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0607560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0732120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0732120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0732120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411079"/>
    <dbReference type="OMA" id="YLDLMEC"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1366">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0732120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="486" mass="52357" checksum="9762FBC443B9FD29" modified="2022-05-25" version="1">MAPQHFLVVAFPGQGHINPARALAERLARATPGARVTLSAAVSAHRRMFPSLASPDDEVHDGAISYVPYSDGYDHGFSLFAGDGDEAERYAEAFGRVGRETFSAVLDRLAARGRPVTCVVYAMLMWWAAEVARERGLPRALYWIQPATMLAVYYHYFHGYERTVTEHAAEPGFTVSMPFLPPMEIRDLPSFFTNLTDGRLAAAFGGIRRTFQQLDLDVGSGGSGAGGSRRAMVLVNTVEELESGALASVPELDVFPVGPAVVSLFTEGEGGTSSGTAAAAAVGDLFEHDEQGYMEWLDTKPARSVAYVSFGSMSAVSKRQKDELRRGLAASGQPYLWVVRNNNRDDGFDAAGDVRGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDTNARLVVQWGVGVRAATDVDRLLVAEELARCLEMIMGDTEEGAAIRASSAAWKAKLRQAIADSGSSGRNLRIFLNQFANDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNA4</accession>
    <name>A0A8I6YNA4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNA4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0431200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0431200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0431200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0431200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0431200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0431200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389">
        <property type="entry name" value="OS09G0436400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389:SF5">
        <property type="entry name" value="OS09G0436700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0431200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="242" mass="27143" checksum="511558733AFD5D69" modified="2022-05-25" version="1">MPKAGKRRRRVNPETETAARKKEKKTTMRSLAEDRSLTFAAFQSEKAKPQAKLDPVYDDVDSSDEESSSCPPSPLLHRPYILNELADNPDIRAAFHLANTEYHADRACRSAVLDCYSSASCLSNNPSLLHIREPAKDAVLLAADSIITLSSYLDADDDEPFNTCCGLWIQHDIKKKTAVVLTSAHLIRAKDPSVRHQWMLRSTCEYHREAEVIVHLLDGETAVASLLYLQEHYEFALYEGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQ55</accession>
    <name>A0A8I6YQ55_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123403781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953627.1">
        <property type="nucleotide sequence ID" value="XM_045097692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQ55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553430"/>
    </dbReference>
    <dbReference type="GeneID" id="123403781"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0459740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0459740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0459740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0553430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403781"/>
    <dbReference type="OMA" id="IINMHSV"/>
    <dbReference type="OrthoDB" id="419711at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF22">
        <property type="entry name" value="OS02G0130600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="344" mass="39148" checksum="89922001678145A4" modified="2022-05-25" version="1">MRFTAGGSSTRSWQPRPTADTTDLGFWLRWRVAVCALWVLCCVAAAAYLVWRHEGPRAHRRPGGAAKHDAAATQGRRPDGLLYDDEAWRPCLRDIHPAWLLAYRLVSFFVLFSLLIVIVISDGGNIFYYYTQWTFILVTVYFGLATTLSIYGCSKFAACNAVAAMSDAEQGPYAIHGAAPKPVVDGEDDGTREVAGFWGYLLQIIYQTNAGAVMLTDCVFWFIIFPFLTVKDYSMNFLLIGMHSVNAVFLLGEASLNSLRFPWFRIAYFFLWTALYVVFQWIVHASTPTWWPYPFLDLSSNLAPLWYFAVAFMQLPCYLIFRLVMNLKHHLLSKHFPDSMVLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YS29</accession>
    <name>A0A8I6YS29_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123410393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044959265.1">
        <property type="nucleotide sequence ID" value="XM_045103330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YS29"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740120"/>
    </dbReference>
    <dbReference type="GeneID" id="123410393"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0613890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0613890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0613890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0740120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410393"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF31">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="7">
        <location>
            <begin position="24"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="8">
        <location>
            <begin position="347"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="581"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="66509" checksum="8416AF64D4FC3F80" modified="2022-05-25" version="1">MAGGEGAAAVMCTPAFVGRVLRSRWYVVFASMVVMAASGSTYIFALYSKELRSVLGYNQQTLNTLGFFKDLGTNVGVVSGLVQQVAPTWAVLLIGAGMNLAGYLMVYLALTERTAAPPVWLMCIYMCVGANALTFSNTGALVACVKNFPESRGIVIGLLKGFVGLSGAIYTQLYLAIYGDDAKSLVLLIAWLPAAVYIFFVHTIRVLPYRRRAEGDEPNSRPFFCFLYISIALATYLLVMIVVQKQVPSFSHAAYAVGATVLLLILFLPLGVVIKEEYTAVSQLEESLQHPPAIAVEEPAASSAAKDKDKDDDGRDDPKCGGITGCLTVTNMFKPPALGEDYSIMQALVSVEMLVLFVVSVFGIGGTLTAIDNMAQIGQSLGYPPKSINTFVSLISIWNYAGRVGAGYMSEFFVARYRFPRPLALTAVLLVSCVGHLLIAFGVPQSLYAASVILGFCFGAQWPLLFSIISEVFGLKYYSTLFNFGSAASPIGAYVLNVRIAGRMYDAEAARQHGGNAAAVGDKICKGVTCFKHAFLIITGVTLAGVLVSLVLVWRTRNFYKGDIYAKFKVAPVVVTGTAADGSDRGREMVEGTATVTEDGGAKEGKKKKQDQVNDEEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YSI9</accession>
    <name>A0A8I6YSI9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Methyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123398238</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_044948667.1">
        <property type="nucleotide sequence ID" value="XM_045092732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YSI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0484260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0484260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0484260"/>
    </dbReference>
    <dbReference type="GeneID" id="123398238"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0400900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0400900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0400900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0484260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0484260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0484260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398238"/>
    <dbReference type="OMA" id="KPCHPRY"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000122">
        <property type="entry name" value="probable methyltransferase PMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1119">
        <property type="entry name" value="METHYLTRANSFERASE PMT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0484260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="614" mass="68836" checksum="26A16FB4653B7B64" modified="2022-05-25" version="1">MAVKGGPADNRTRSTVSICIVIGLCCFFYILGAWQKSGFGKGDSIALEITKRTDCTLLPNLSFDTHHSKQGSSGDLVSPVKKFKPCPDRFTDYTPCQDQNRAMKFPRENMNYRERHCPPQKEKLHCLVPPPKGYVAPFPWPKSRDYVPFANCPYKSLTVEKAIQNWVQYEGNVFRFPGGGTQFPQGADKYIDQLASVIPIANGTVRTALDTGCGVASWGAYLLKRNVLAMSFAPRDSHEAQVQFALERGVPAVIGVLGTVKLPYPARAFDMAHCSRCLIPWGMNDGLYMMEVDRVLRPGGYWVLSGPPINWKANYKGWQRTKEDLQAEQNKIEEMAELLCWEKVSEKGETAIWRKRVNTESCPSRLEESTVQMCESTNADDVWYKKMKACVTPLPDVENPSEVAGGAIKPFPSRLNAIPPRISNGLIPGVSSEAYQKDNKMWKKHVKAYSNVNKYLLTGRYRNIMDMNAGFGGFAAAIESPKSWVMNVVPTVAKIATLGAVYERGLIGIYHDWCEAFSTYPRTYDLIHASGLFTLYSNKCSMEDILLEMDRILRPEGAVIMRDDVDVLTKVNKLARGMRWNTKLVDHEDGPLVREKVLYAVKQYWVGGNQTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVJ1</accession>
    <name>A0A8I6YVJ1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123402859</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Probable histone demethylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVJ1"/>
    <dbReference type="SMR" id="A0A8I6YVJ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0511950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0511950.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0511950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0617270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052901">
        <property type="term" value="F:spermine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046208">
        <property type="term" value="P:spermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903602">
        <property type="term" value="P:thermospermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000064">
        <property type="entry name" value="Lysine-specific histone demethylase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF381">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="6">
        <location>
            <begin position="178"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="868" mass="92812" checksum="D3A6B4240C030D5A" modified="2022-05-25" version="1">MGLFPPESRPSLGHRTVLALNQPLNPPPNSQIQSPSSPHPSPETPMDEANEAQPPPDAPEAAGHLALVPMEQDEAAAAAEPMEDDAAGGDAAASAEPMEEDAPTSSPTPSAPSTTAAVDDSTVARKRRRRKKQFPGMIPTAGVRVLRASSSSGATAAHLAGIPRRRGRPPTNSSLRLARELDSEATIALAAGFPADTLSEDEVAAAVIPVIGGTDQANYLVVRNHILALWRSNPLSPVASNAALASIRAEHAPLVAAAHSFLSDHAYINFGLAPSIVSLPPIPPPSHPPPSVLIVGAGFAGLAAARHLMSLGFKVAIVEGRLRPGGRVFTKTMRSSAADYPDIAASADLGGSVLTGINGNPLGVIARQLGFPLHKVRDKCPLYLPDGRPVDPDMDGRVEAAFNQLLDKVCQLRQVIADSVPHGVDVSLGMALEAFRAAHGVAAEPEERMLLDWHLANLEYANAAPLADLSMAFWDQDDPYEMGGDHCFIPGGNSQFVRALADGVPIFYGQNVRRIQYGCDGVLVYTDKQTFRGDMALCTVPLGVLKKGDIDFVPQLPAQKREAIQRLGFGLLNKVVMLFPFDFWDGRIDTFGHLTEDSSQRGEFFLFYSYSSVSGGPLLVALVAGESAISFEKKSPMENVEKVLDTLKKIFSPMGIEVPNPLQAICTRWGTDRFSYGSYSHVAIGSSGDDYDILAESVADRVFFAGEATNRRYPATMHGALLSGYREAANIVRAARKRAKKVDSSEKIDIIYEVRDIVKDDNIDLDDLFRTPDVAFGGFSVLHDPSISEPDSASLLRVGIGARKLGSGSLFLYGLIMRKNVTELAAMEGDEQRLSTLYRDFGTKLVGLDGLGDAGESLISRIKAASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z155</accession>
    <name>A0A8I6Z155_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z155"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0668080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0668080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0668080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0668080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0668080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0668080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052160">
        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0668080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="23782" checksum="5B8212FE09A8235D" modified="2022-05-25" version="1">MMLKEVIFPRFGVPRYLMTDGGSHFIHGAYRKTLGKYDVNHRIASPYHPQSSGQVELSNREIKLILQKTVKRSRKNWCKKLDDALWAYRTAYKNPMGLSPYKMVYGKACHLPLELEHKAYWAIKELNFDFKLASEKRLFDISLLDEWRTQAYQNAKLFKEKVKRWHDKRIQKREFNVGDYVLLYNSRLRFFAGKLLSKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z3A5</accession>
    <name>A0A8I6Z3A5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123403103</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953004.1">
        <property type="nucleotide sequence ID" value="XM_045097069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z3A5"/>
    <dbReference type="SMR" id="A0A8I6Z3A5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611780"/>
    </dbReference>
    <dbReference type="GeneID" id="123403103"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0507290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0507290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0507290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0611780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611780.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123403103"/>
    <dbReference type="OMA" id="LSDTNWF"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF43">
        <property type="entry name" value="OS02G0729100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="12">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="11">
        <location>
            <begin position="116"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="46224" checksum="27C25A15153B739F" modified="2022-05-25" version="1">MKSHQSKSGPSSRKRSAAVAAPVAFLLVLVLVTLYDLTFADRRYLRIHGASSSSSSSSSSPACDLTRGEWVRDAAEAPYYTNLTCPFIDDHQNCIKFGKPSLEFLRWRWRPDGCELPRFDAARFLHAMRGKSMAFVGDSLARNHFKSLLCLLSRVAQPVEVGGEPEIDPTGRAVRRDYYYGSHDFTASLFWSPFLVKANLSNATLGMWDVHLDTPDARWASHLAGFDHVVLSGTNWFLRPSVYYEGGRAVACNGGASCVAGNVSAEMTVPRALRAAFRTALGAVAASEGFRGKAVLRTVTPAHFEGGEWNTGGDCVRTHPYRRGDRALGAVEAEYRSAQVEALRETKSARTRRDGSEAELVLMDITGAMDLRPDGHPSRYGHPPGGSVEGSFVVDCLHWCLPGPIDLWSELLSHMLLHPRP</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC123412231</name>
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    <organism evidence="3 4">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961107.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z674"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="16"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="225" mass="24899" checksum="88F889A78460BE8E" modified="2022-05-25" version="1">MTILSKDAPPEISHPAHPEHKLKLMATGAAEFQCDVCKELGAGDRYTCRPCDFDLHSDCALAEATLVHPLLKGREFQLRHEHPRHSDGGGRTCGACGGKVLGLHYHCAAKKGLDLHPCCAALPLVIPQEELTLELRKEASHRCSSCRERDRSRSRSRTWFYRSTCKTVYLHVACVKEIARRSRGAGVGSSIDPFASVKESALQIYRAKKDESELERVILELVLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z690</accession>
    <name>A0A8I6Z690_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7769 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z690"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0715880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056671">
        <property type="entry name" value="DUF7769"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33889:SF1">
        <property type="entry name" value="OS03G0834800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33889">
        <property type="entry name" value="OS04G0681850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24964">
        <property type="entry name" value="DUF7769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7769" evidence="1">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="28738" checksum="9F367959667DB40C" modified="2022-05-25" version="1">MEGDEIVEDTVVEQVAKRNKTLNDQQKFAAYVALHTLCMSRGGILKRTDTQDIANFFGVGVWNIQRIWRKAMSQIKQGQEVDVSNQRKGNCGRKPKDINLEQIITIPLNRRSTIRSLAWQLGCSPTMLHRKFMLKLIRRHTYCVKPALKEKNKKNRMKFCLSMLDEATTTTPRPKFKTMHNIVHIDEKWFNMTKKNKTYYLLDGEEEPTRPIHGSCIGKVMFLIAIARPRWDSEGNVTFCRKSVSGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4D4</accession>
    <name>A0A8I7B4D4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PI-PLC X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123428522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968683.1">
        <property type="nucleotide sequence ID" value="XM_045112748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4D4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215680"/>
    </dbReference>
    <dbReference type="GeneID" id="123428522"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0215680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428522"/>
    <dbReference type="OrthoDB" id="7984201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08588">
        <property type="entry name" value="PI-PLCc_At5g67130_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF48">
        <property type="entry name" value="OS04G0689300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035308666" description="PI-PLC X domain-containing protein" evidence="2">
        <location>
            <begin position="31"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="62364" checksum="B9FF826F1D8C4BB7" modified="2022-05-25" version="1">MAAVAVGMARRSGTLLLLLTLAVVAGTASGSALVGDRCAAGSQSPCGAGMWCASCSPLAGSGTAVCSRITPIDPKTHGTGLPFNKYSWLTTHNSFAMAGTTSPSGAPIVSPPNQEDTVTNQLKNGVRGLMLDTYDYKNDLWLCHSFSGKCFEVTAYQPASKVLKEVEGFLNANPDEVVTVFVEEYSAPGSLGKALSAAGLTKYLFPPASMPKDGADWPTLKDMIARNHRLLVFTSKQGRQGSDGAAFEWDYVVETQYGSDGLAVGACPKRAESKPMDSKGQSLVLLNFFTTNPSQSWACVNNSAPLVSKLRACYDASAKRWPNYIAVDFYMRSSGGGAPLATDVANGRLQCGCDSIAYCKANATFGTCALPSSPLTPAPSSSPSPSPPSASSSSKFSASARSPSSASSPKGSPGPSSSPSGSWLPSPAPARSWSWSSSPALSLFSFMDSSMELSPPESLSLSPSLAPSSWPSLGSAVSSTLSPLPSPSASSSSSPRRSKKSPKSTINVMSVNELLPESTTTDEGAAAKASPKSTPNQGIAPEASPNGTPESGAPQRTLPKSIAEPPTPTSHPEAKDASSAAIRPKTPRRSSLIGTALLVLISLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7Q4</accession>
    <name>A0A8I7B7Q4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Expansin-like A2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123447309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979835.1">
        <property type="nucleotide sequence ID" value="XM_045123900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7Q4"/>
    <dbReference type="SMR" id="A0A8I7B7Q4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409760"/>
    </dbReference>
    <dbReference type="GeneID" id="123447309"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0341280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0341280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0341280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0409760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447309"/>
    <dbReference type="OMA" id="MAMEING"/>
    <dbReference type="OrthoDB" id="623266at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22276">
        <property type="entry name" value="DPBB_EXLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF98">
        <property type="entry name" value="EXPANSIN-LIKE EG45 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035205443" description="Expansin-like A2" evidence="5">
        <location>
            <begin position="26"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="46"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="166"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="28690" checksum="BC83EDB5BB861D1E" modified="2022-05-25" version="1">MAVSLGSILLLPLLFLSTLPPPASACDRCVHRTRAAYYTSSLTLAAGSCGYGAAAASLNGDLLAAAGPSLYRQGVGCGACFQVRCKDEELCSTAGVKVVVTDRASTKTNDTELVLSSPAFAAMARPGMAARLAKLGAVEVEYKRVPCEYEGKNLSVRVEERSRAPSELAITILYQGGQTDIVEVDVAQVGSSSSWSSLTRDHGPAWSTSLAPPGPLQLRAVVTSGFDGSWVFAEHEVLPRQWHAGEVYDTGVQITSIAQEACFPCDTQEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7Z2</accession>
    <name>A0A8I7B7Z2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF642 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123427607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
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        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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    </evidence>
    <sequence length="386" mass="39570" checksum="EBECA01A0491BF59" modified="2022-05-25" version="1">MVVNGARCVAALLLLAFTAARAVPDVDGPLLNGNFEYPPNRSQMNGTRVTGAHAIPYWKATGPVEYVESGTDQPGDGMVLPVPEGAHAVRLGSGASVRQQLSLTRGACYSVTFSAARTCGQSERLRLSVVPVDAADAPGRELPIQTVYSASGWDSYAWAFHAEQGVVTLVIHHGDGGGAAEDPACGPLVDAVAIKTLHPAAQAAGGNLLRNGDFEEGPYVAPGSACGVLVPPMDADAMSPLPGWMVMSYSKAAKYVDAAHYAVPCGSRAVELIAGVEAALVQEVDTVPGSACRMEFSVGDAGDGCVACGPEQQPMRVVAAATGAQGPATVEYHSSGKGGHARGELAFTAEGNRTRVVLYSSGYHTMADGSGSLCGPVVDDVSLVCT</sequence>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="27140" checksum="50E17AA7D71A0952" modified="2022-05-25" version="1">MATFIHVKFTEFCQPDATMPFEKELVQITKDLRIALPTITGKPTSSYPEDSRYRIEVHVPGRTFQPRTEPMDFKFITPNWILGRDMAIHCALGRIKEEYKGFPISPDLFTVSRRNKDGEIIPSKTKDALLSYAQTLEKHSGALEHRVIKDARKIKQLSLRELELEEAAWEAHYEHELEAKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPTTMLF</sequence>
</entry>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Mascher M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
    <sequence length="95" mass="10696" checksum="D031F36766F8BDE1" modified="2022-05-25" version="1">MKYVEVESNCQTLIKALQSSVYVRAPEGILLREIWDFARLSFSTCTFYFVPKACNNLVHALVAFGASQPAGLHLWLEDLPDKVLVRRDSALVKPV</sequence>
</entry>
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    <accession>A0A8I7BDI5</accession>
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            <fullName evidence="7">carotenoid 9,10-dioxygenase</fullName>
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        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>all-trans-zeaxanthin + 2 O2 = 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial + 2 (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:26393"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:27547"/>
            <dbReference type="ChEBI" id="CHEBI:53171"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
            <dbReference type="EC" id="1.14.99.n4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.n4" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044946269.1">
        <property type="nucleotide sequence ID" value="XM_045090334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDI5"/>
    <dbReference type="SMR" id="A0A8I7BDI5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0419880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0419880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0419880"/>
    </dbReference>
    <dbReference type="GeneID" id="123395340"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0349440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0349440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0349440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0419880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0419880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0419880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395340"/>
    <dbReference type="OMA" id="RYVYCAI"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF89">
        <property type="entry name" value="CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0419880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="551" mass="61859" checksum="9A576379029E71C2" modified="2022-05-25" version="1">MGEAVAVAAAEDAMKKKGAVVVPAPQPSKGVASWAVDLLERIAVRLGHDKAKPLHWLSGNFAPVRDETPPAAGLPVRGHLPECLNGEFVRVGPNPKFVPVAGYHWFDGDGMIHAMRIKDGKATYVSRYVKTSRLKQEEYFGGAKFMKIGDLKGFFGLFMVQMQALRKKLKILDVTYGFGTANTALIYHHGKLMALSEADKPYVVKVLEDGDLQTLGLLDYDKRLKHSFTAHPKVDPFTDEMFTFGYSHEPPYCTYRVITKDGVMLDPVPITIPESVMMHDFAITENYSIFMDLPLLFRPKEMVKNGEFIYKFDPTKKARFGILQRYEKDEKTIRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDKVNGTQNEKLENFGNELYEMRFNMKTGAASQKQLSVAAVDFPRINESYTGRKQRYVYCTILDSIAKVTGIIKFDLHAEPESGKKQLEVGGNVTGIYDLGPGRFGSEAVFVPKEPGVSGEEDDGYLIFFVHDENTGKSEVNVIDAKTMSADPVAVVELPNRVPYGFHAFFVNEEQLVHQQAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFH4</accession>
    <name>A0A8I7BFH4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410681</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959556.1">
        <property type="nucleotide sequence ID" value="XM_045103621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFH4"/>
    <dbReference type="SMR" id="A0A8I7BFH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0738880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0738880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0738880"/>
    </dbReference>
    <dbReference type="GeneID" id="123410681"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0612860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0612860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0612860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0738880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0738880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0738880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410681"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670">
        <property type="entry name" value="HEAT SHOCK PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670:SF114">
        <property type="entry name" value="OS05G0592000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="7">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0738880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="36179" checksum="962C7877212F6870" modified="2022-05-25" version="1">MAAATNTKQKQQQQPPPPAGGQPVVVDPKFEWAEKAGGYVLRITLQGFKKGDFRVQVDGAGRLTVSGARPGTTPGSLHKVFQLPSTASLDDIAGRFEAGVLTLTVPKRAYSGVPAPTSVEEIKRDKPGVVKDDGAPSTPVVTPPANKDVAGEEKKAAGGMKSKEEIGKEEDVSKKAMDEAAMKAQQQKQPEEASKQKEEQQKLAPEPEAKKEQEVKPKAPQAAAPAAPEKPAQKPEVFNGEKAKAVVDQESLAAVVRRRSEEEKAKAAAAAMEAKAVLEKKATACGGWKERVAGGLEQLTDMKWADNVVEKARNNKEVIAIAIAAFSVGFFVSQKLFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSH2</accession>
    <name>F2CSH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123452150</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AK354574">
        <property type="protein sequence ID" value="BAJ85793.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK369071">
        <property type="protein sequence ID" value="BAK00273.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984682.1">
        <property type="nucleotide sequence ID" value="XM_045128747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CSH2"/>
    <dbReference type="SMR" id="F2CSH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509180"/>
    </dbReference>
    <dbReference type="GeneID" id="123452150"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0422870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0422870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0422870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452150"/>
    <dbReference type="HOGENOM" id="CLU_130032_0_0_1"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000005">
        <property type="entry name" value="Prefoldin subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ85793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK00273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="14488" checksum="0212C68E1807B2E2" modified="2011-05-31" version="1">MQQGDGTEAQVTWDDQQNINRFGRLNNRLHELADEIRLAKEANENLEDAGNELILSDEDVVRFQIGEVFAHMPVDDVETRLEQMKEDAAKKLEKLEEEKESILAQMAELKKILYGKFGEAINLEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXJ3</accession>
    <name>F2CXJ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123430626</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356346">
        <property type="protein sequence ID" value="BAJ87564.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359647">
        <property type="protein sequence ID" value="BAJ90856.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970416.1">
        <property type="nucleotide sequence ID" value="XM_045114481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXJ3"/>
    <dbReference type="SMR" id="F2CXJ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186470"/>
    </dbReference>
    <dbReference type="GeneID" id="123430626"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0154380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0154380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0154380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0186470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430626"/>
    <dbReference type="HOGENOM" id="CLU_096624_0_0_1"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34123">
        <property type="entry name" value="OS04G0578200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34123:SF1">
        <property type="entry name" value="OS04G0578200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ87564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ90856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="239" mass="26470" checksum="7C2656FC93127D6F" modified="2011-05-31" version="1">MASLHRAPATSLPAGHHRQGAGALLQRPCGRRSRLNAQKKPGSTRPRTGTPSGSESDNVVLKAAWYGSEVLGIAASFFRPPSPEADAGAADDGASQSQPSGRFQVAEAIKDDFARSYFVTGNLTLGAYEDDCEFADPAGSFRGLGRFKRNCTNFGSLLEKSNMKLTKWEDLEEKSIGHWRFSCVMSFPWRPILSATGYTEYYFDGESGKVCRHVENWNVPKMALLRQIFRPSKWAWEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZ63</accession>
    <name>F2CZ63_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123431070</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356919">
        <property type="protein sequence ID" value="BAJ88134.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970819.1">
        <property type="nucleotide sequence ID" value="XM_045114884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZ63"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206030"/>
    </dbReference>
    <dbReference type="GeneID" id="123431070"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0171400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0171400.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0171400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0206030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431070"/>
    <dbReference type="OMA" id="IDCCVAF"/>
    <dbReference type="OrthoDB" id="702784at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090588" evidence="1">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ88134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="112" mass="11614" checksum="BF156B5619C9BA12" modified="2011-05-31" version="1">MAAAMLPRLVAVAALVALMCAVAVAQAPAAGGAPVCEGVDQNVVNACFKSFGEGMKIAIADRKLSEGNVIKVKIDCCVAFGGHSCLCKMKKVWKAQSKSAQDNVQCVREKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5K8</accession>
    <name>F2D5K8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123410901</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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                <person name="Amano N."/>
                <person name="Kanamori H."/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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            <strain evidence="11">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0710190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0710190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043424">
        <property type="entry name" value="BLT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071">
        <property type="entry name" value="GB|AAF24581.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071:SF9">
        <property type="entry name" value="INTRACELLULAR PROTEIN TRANSPORT PROTEIN USO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ96175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0710190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="738" mass="82958" checksum="27CE11A02B2F232F" modified="2011-05-31" version="1">MEVEPPAAPLQKCGVGGEQPAHGTPNRQREPEEGAPLRRELAAARARRRADRATPSPSWKLEASPPRPEEPAADAAAAGRRSSSGASARQLGATLWEIQDVIRAAGAGRRIRRRARRGPAASPLHGGDDDVDRPHISGGCGRDLSAPLMEHEKLHEDRCHSRQSLSPASYTSSIGAATINLVSPTRSLNFRDRYRDAGCNLKTSTELLKVLNRIWSLEEQHAVDVSTVKGLKLELQNTQARVQELTQERQRYHYEIESLARQVTEDKMARKNKEQEKLRATLRSLQDELEAERHLRKHSENLHRKLGKELSAMKPAFLKAVKDLEKEQKATRLLEDLCDEFALGIRNYEEEVRVLKQRHVKEYEHKTDKLVVHISEAWLDERIQMQNADARGDSEGKTSITERLSGEIQSFLHGRRSSNLYGVNKHTGNEKGDASLCRQSLESLHLNGATSAPQLAEDDENSVASDLHCFELDMHGDAVQTHELAGTRRTVADCMYSPMRRLEFSDGVSVEGSRISTARPRSEKGKVKPSSSKAQLNTSTPEISSRNCDKIDPIDEQNETVMTQVSRRLHDDLLKIKSEAPQHAFLERKPHDHHPRTYQFRECATSSDLLHNLHSPARQLKNHQRASLDYQRSECATTGDLLGLRSPSWQLKNQRASLDYQMSECATTGDMHNLRSPSRQQKNHHRSTLDHEISESSPARSLGSKDNTLKAKLLQARLEGQHARIRASGYPLISTRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2DM63</accession>
    <name>F2DM63_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123443692</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK364981">
        <property type="protein sequence ID" value="BAJ96184.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK367194">
        <property type="protein sequence ID" value="BAJ98397.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976127.1">
        <property type="nucleotide sequence ID" value="XM_045120192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DM63"/>
    <dbReference type="SMR" id="F2DM63"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0265190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0265190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0265190"/>
    </dbReference>
    <dbReference type="GeneID" id="123443692"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0219910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0219910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0219910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0265190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0265190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0265190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443692"/>
    <dbReference type="OrthoDB" id="26838at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06148">
        <property type="entry name" value="Egl_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46814">
        <property type="entry name" value="EGALITARIAN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46814:SF1">
        <property type="entry name" value="EGALITARIAN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="251"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ96184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0265190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="332" mass="37181" checksum="D99C1A9EE7A34767" modified="2011-05-31" version="1">MTSHPPNSADGEDPAGNNVELPSLPFHVVTKPGQLPVEFLEPSAAKKLVIGFDCEGVDLCRNGALCIMQIAFPDAVYLVDAIEGGKELVEACKPALESDHVTKVIHDCKRDSEALYFQFGIKLHNVMDTQIAYSLIEEQEGKKRAYDVYISFVSLLADPRYCGMPYPEKEEVRTLLRQDPNFWKNRPLSEMMIRAATDDVRFLLNIHEKMMEKLSKVSSWRLAVRSELYCRCFCINDNQQADWPPLPTVPDDIEAEVRVPEVDILSLLDVPPGKMGRVIGRKGSSIMAVKESCNVEIHIGGAKGPPDRVFIIGPVKEVRKAEAILRGRMLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMR1</accession>
    <name>F2DMR1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRISC and BRCA1-A complex member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">BRCA1-A complex subunit BRE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">BRCA1/BRCA2-containing complex subunit 45</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123408748</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the BABAM2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK365179">
        <property type="protein sequence ID" value="BAJ96382.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957730.1">
        <property type="nucleotide sequence ID" value="XM_045101795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMR1"/>
    <dbReference type="SMR" id="F2DMR1"/>
    <dbReference type="STRING" id="112509.F2DMR1"/>
    <dbReference type="PaxDb" id="4513-MLOC_55955.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721880"/>
    </dbReference>
    <dbReference type="GeneID" id="123408748"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0598710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0598710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0598710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0721880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408748"/>
    <dbReference type="eggNOG" id="ENOG502QUU0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="538811at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DMR1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010358">
        <property type="entry name" value="BRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15189">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15189:SF7">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06113">
        <property type="entry name" value="BRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ96382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="391" mass="43206" checksum="545CD58BFD471E44" modified="2011-05-31" version="1">MAPSAAAAAAASAAPALAPPLAPLIAAQLKFLLTNSSLPIKVVQIWSGCGNGRYADRFTLAVPFCLDYVYWDFLYNAMHPKVAPDVIFGQGDEGFQPLVDYDESGNGGKSCLAHWDYRDPRGLLCLVEELRLLYIEYQKKLVAEVGDTRLKFEIGTVLDKEGIEVSMVSSTGRPDEVKFAVPLLDLDLNMMVPGCPWKLPQKIHLQAIFPVSSSFLSVPSAPGLKLISTPDLKSLFSVDGVKLPAWVDGMCMAEYIPSLEENLKMQVVDASASIGRRRRFIEALAPAFGRPLEADPIFCRKATVLSISGIFTFLVHFVIPLQFPQQQPILTLQSCQHCNSQGIPITSPPKNSYPWSPRWEVTEMVERIYDYLADECQNFKKQCSDGFPQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2DP04</accession>
    <name>F2DP04_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123404644</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK365622">
        <property type="protein sequence ID" value="BAJ96825.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044954517.1">
        <property type="nucleotide sequence ID" value="XM_045098582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DP04"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558240.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558240.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558240"/>
    </dbReference>
    <dbReference type="GeneID" id="123404644"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0463730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0463730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0463730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0558240.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558240.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404644"/>
    <dbReference type="OrthoDB" id="2610923at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="11">
        <location>
            <begin position="129"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ96825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558240.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="775" mass="86782" checksum="8805053B43879708" modified="2011-05-31" version="1">MDGCLALGFDQIVFLSMGEPKSNDNAMLHNGEMMMISSSEVVHCSEMLHGDNMVQESEILHGDEMVISGNEMVHGSEMIHGNEMVQVNDMIRSNDMDRVNNMVNGDEMAHGNALVSADLSPPTSSRRRKKKSPVWEHFTIQHVPGDKRCRIACCNLCKGTFAYSSGGKIAGTSHLKRHILQGSCPVIKNQERELALPLAEVAGNVGEGAIERPSKRRYRQTGFANATFDQDRSSLDLAKLIILHEYPLHVVQQPAFTALIGSLQPCFRVSDVDTMEGEVYAVYQKEKHNLLQALSTIPGRMSLTMGLWITSQTLGYVSLVGQFIDTDWRVHRRVLNFTMVASPHSQDALAEAISRSLFDWGMKEKLFAITLDNDCSSHDIYSANLRDHLSNKNGVMLKGQLFVVRCYAHILNAVAQDVIASAHGVIFSIRESIKFIKASDSYDQKFTEIALQLEIPSTKILCLDVTTQWDTTYLMLLAALDFRQVFTILETCDDNYNEAPSAEDWKKVEAASNCLKLLYDSAHSVMAVANPTSDIFFHEAWKLQLELANAAAHEDLVVSSTARDMHESFDKYWKDCNLVLAVAVVMDPRFKMKLVEFSYSKIYGVEAAKYVKVVNDSLHELYKEYLAQPLPARPVYVGQSASGNNNTQTTPPSIGDGLLDFDMYLSEIATAQPSKSELEQYLEEPLTPRSIQEFDILSWWKLNTLRFPTLSKMARDVLAIPMTMVSSIFSAETGIRTLDDYRSSLRPEIVEALVCAKDWLLHSPPASQTQSREMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F2DRD7</accession>
    <name>F2DRD7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">riboflavin kinase</fullName>
            <ecNumber evidence="2">2.7.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123430407</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="2"/>
    <dbReference type="EMBL" id="AK366455">
        <property type="protein sequence ID" value="BAJ97658.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK369956">
        <property type="protein sequence ID" value="BAK01157.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK374913">
        <property type="protein sequence ID" value="BAK06109.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK376753">
        <property type="protein sequence ID" value="BAK07947.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970222.1">
        <property type="nucleotide sequence ID" value="XM_045114287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DRD7"/>
    <dbReference type="SMR" id="F2DRD7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0040860"/>
    </dbReference>
    <dbReference type="GeneID" id="123430407"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0040860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0040860"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_3_1_1"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DRD7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006771">
        <property type="term" value="P:riboflavin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000119">
        <property type="entry name" value="Bifunctional riboflavin kinase/FMN phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.30:FF:000005">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749:SF6">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="8">
        <location>
            <begin position="234"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ97658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK06109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="397" mass="43848" checksum="F8C1FF61821C89A0" modified="2011-05-31" version="1">MAAPKPITRLISHVILDLDGTLLNTDCIVSQVLKPFLVKNGKRWDSKNAHKYVGKTPYEAAAVVLEDYGLPYSTEEFLSLINPMFSEQWGNLKALPGANRLIKHLKSSRVPAAIASNSSRSNIESKISCHQGWKEYFSALVGADEVELGKPSPEIFLEAAKRMNADPSNCLVIEDSVPGVTAGKAAGMHVIAVPSVPKRTDEFSSADEIINSLLDLKPEKWGLPPFNDWIEGTLPIDPWFIGGPVIKGFGRGSKVLGIPTANLAAENFSDILSEHTSGVYFGWAGLSKRGIYKMVMSIGWNPYFDNTEKTIEPWLLHGFDEDFYGEELRLVIVGYIRPEANFPSLESLIERIHEDGRIAENALDLPEYAKYKDSPYLRNPLQQGASADGNEAEPEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTJ3</accession>
    <name>F2DTJ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123443719</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367211">
        <property type="protein sequence ID" value="BAJ98414.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976153.1">
        <property type="nucleotide sequence ID" value="XM_045120218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTJ3"/>
    <dbReference type="SMR" id="F2DTJ3"/>
    <dbReference type="PaxDb" id="4513-MLOC_15683.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0266190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0266190"/>
    </dbReference>
    <dbReference type="GeneID" id="123443719"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0220710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0220710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0220710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0266190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0266190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0266190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443719"/>
    <dbReference type="eggNOG" id="KOG4018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084528_2_0_1"/>
    <dbReference type="OMA" id="QWDEHKK"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DTJ3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000071">
        <property type="entry name" value="RWD domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="10"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ98414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="247" mass="27969" checksum="B4EF8EC6A472B489" modified="2011-05-31" version="1">MADYEQEQEMELEALQAILMDDIKEIDPSESGIDTNSRCFEILLSPQDDDFDEAAHVPVQMALVFAHTEKYPDEPPLVNIKSVRGIKPDDLTSLKEKLDQEATENLGMAMVYTLLDSAKEWLTEKYGQNAGDEEPDETEEPAEEVIIPHGEAVTVESFMAWRERFEAELALQRAKLMPESALTAPKEKKLSGRQYFESGRHITKGAGTVAEEEEEEEEDIEFDDDFEDDEEDMLEHFLAEQTGKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZE8</accession>
    <name>F2DZE8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123402996</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369268">
        <property type="protein sequence ID" value="BAK00470.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952907.1">
        <property type="nucleotide sequence ID" value="XM_045096972.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952908.1">
        <property type="nucleotide sequence ID" value="XM_045096973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DZE8"/>
    <dbReference type="SMR" id="F2DZE8"/>
    <dbReference type="STRING" id="112509.F2DZE8"/>
    <dbReference type="PaxDb" id="4513-MLOC_6251.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0589440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0589440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0589440"/>
    </dbReference>
    <dbReference type="GeneID" id="123402996"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0488540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0488540.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0488540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0589440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0589440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0589440"/>
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    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_041852_0_0_1"/>
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    <dbReference type="OrthoDB" id="45365at2759"/>
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        <property type="component" value="Chromosome 6H"/>
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        <property type="expression patterns" value="baseline and differential"/>
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    <dbReference type="CDD" id="cd22152">
        <property type="entry name" value="F-box_AtAFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
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    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF27">
        <property type="entry name" value="KELCH REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK00470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0589440.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="460" mass="51342" checksum="371FE7142919F442" modified="2011-05-31" version="1">MGSYLSTATSKTRSVDSDEVHGSVLNKRAKITTYDCGSYSRIIPTLPDELSFQILARLPRIYYLKMKMVSRTWKAAITGSELAQLRRELGLTEEWLYILTRVEANKLECYALDPLFQKWQRLPSMPLFANEADSTGRTRCSAFQMWNVVGSSIRIADFFRGWFCRRYGLDQMPFCGCSVGVADGCLYVLGGFSKAVALDCVWRYDPCHNLWQEVNPMISGRAFSKASLLESKLYVVGGVSRGRNGLLPLRSGEVFDPKTGLWSELPEMPFVKAQVLPTAFLADVLKPIATGMASYNGKLYVPQSLYSWPFFFDIGGEIYDSELNSWSTMPDGLGDGWPARQAGTKLGVVVNDELYTLEPSSSLDSGQIKRYDAEEDVWRTMVPHIPVHDFTDAESPYLLTGLHGRLHVITKEANNNLQVIQAVLENNTGNDVPEGNVLWNIVASKNFGAAELISCQVLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F2E3W6</accession>
    <name>F2E3W6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123409744</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370840">
        <property type="protein sequence ID" value="BAK02038.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958541.1">
        <property type="nucleotide sequence ID" value="XM_045102606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E3W6"/>
    <dbReference type="SMR" id="F2E3W6"/>
    <dbReference type="PaxDb" id="4513-MLOC_57501.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0666630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0666630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0666630"/>
    </dbReference>
    <dbReference type="GeneID" id="123409744"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0553050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0553050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0553050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0666630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0666630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0666630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409744"/>
    <dbReference type="eggNOG" id="ENOG502QU4Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_0_0_1"/>
    <dbReference type="OMA" id="GSCKVVQ"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E3W6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF8">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090845" evidence="4">
        <location>
            <begin position="25"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK02038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0666630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="356" mass="39120" checksum="D3532E3972833F16" modified="2011-05-31" version="1">MGCERKPPLAIALTLALLLGLAHGDVVQFIFGDSLSDVGNNNYLTKSLARAALPWYGIDFGSGMPNGRFCNGRTVADIIGDKMGLPRPPAFLDPSVDETVISKSGLNYASGGGGILNETSSLFIQRFSLYKQIELFQGTQAFMREKIGQAAADKLFGEAYYVVAMGANDFINNYLLPVYSDSWTYNGDTFVKYMVTTLEAQLRLLHGLGARRVTFFGLGPMGCIPLQRLLQRSSTACQESTNKLALSFNKQAGAVIKELSASLPNATFQFGDVYDYFQDIIDRPYMHGFNNSHAPCCTLGKVRPTLTCTPLSTLCKDRSKYVFWDEYHPTDRANELIALETLKRLNITVVANSTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2E7M5</accession>
    <name>F2E7M5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442999</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK372149">
        <property type="protein sequence ID" value="BAK03347.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975146.1">
        <property type="nucleotide sequence ID" value="XM_045119211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E7M5"/>
    <dbReference type="SMR" id="F2E7M5"/>
    <dbReference type="STRING" id="112509.F2E7M5"/>
    <dbReference type="PaxDb" id="4513-MLOC_24056.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066390"/>
    </dbReference>
    <dbReference type="GeneID" id="123442999"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0053020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0053020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0053020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0066390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442999"/>
    <dbReference type="eggNOG" id="ENOG502RIEW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MKEEDWL"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E7M5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044841">
        <property type="entry name" value="LUX/BOA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442:SF29">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="95"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK03347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="305" mass="31364" checksum="F9C5E976BDEF7D2E" modified="2011-05-31" version="1">MREEEEPSWFARFDEDLPAPDELMPLSQSLITRDLAAAFDIPTHTHGGGPLSGADAAGAAEMNGGASSAAGSSGGNGGGNGEEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGLGNGGGGGGGSHSSGSGTDAATEHLFATGPSPFLPPGRGPPGADPYTPYAAMPAAHHHHHHPQQIGHFHHPAARPLGHYGAGPGAGFDHGFLSRAVASGAPVGPPGMHHRMVGGGAAMGMMAPSSFADELELGSRGGAGAGGRRELTLFPTSGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2EDX2</accession>
    <name>F2EDX2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KIN17-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401904</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Seed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KIN17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374347">
        <property type="protein sequence ID" value="BAK05544.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951699.1">
        <property type="nucleotide sequence ID" value="XM_045095764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EDX2"/>
    <dbReference type="SMR" id="F2EDX2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558440"/>
    </dbReference>
    <dbReference type="GeneID" id="123401904"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0463890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0463890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0463890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0558440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401904"/>
    <dbReference type="HOGENOM" id="CLU_030065_1_0_1"/>
    <dbReference type="OrthoDB" id="10266249at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13155">
        <property type="entry name" value="KOW_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2030:FF:000001">
        <property type="entry name" value="DNA/RNA-binding protein KIN17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000021">
        <property type="entry name" value="DNA/RNA-binding protein KIN17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000056">
        <property type="entry name" value="DNA/RNA-binding protein kin17-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2030">
        <property type="entry name" value="DNA/RNA-binding protein Kin17, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056767">
        <property type="entry name" value="C2H2-Znf_KIN17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019447">
        <property type="entry name" value="DNA/RNA-bd_Kin17_WH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037321">
        <property type="entry name" value="KIN17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038254">
        <property type="entry name" value="KIN17_WH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041330">
        <property type="entry name" value="KN17_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041995">
        <property type="entry name" value="KOW_KIN17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805:SF0">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805">
        <property type="entry name" value="KIN17 KIN, ANTIGENIC DETERMINANT OF RECA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25095">
        <property type="entry name" value="C2H2-zf_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18131">
        <property type="entry name" value="KN17_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25092">
        <property type="entry name" value="SH3_KIN17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10357">
        <property type="entry name" value="WH_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01253">
        <property type="entry name" value="Kin17_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Kin17 WH-like" evidence="10">
        <location>
            <begin position="52"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="180"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAK05544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558440.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="421" mass="48083" checksum="FFBB3579946BEEB5" modified="2011-05-31" version="1">MGKGDFLTPKAIANRIKAKGLQKLRWYCQMCQKQCRDENGFKCHCMSESHQRQMAIFGQAPDRVVEGFSDEFLEEFLTLLRRAHRSSRVAATVIYNEFIADRHHVHMNSTRWATLTEFVKFLGREGHAKVEDTPKGWFITYIDRDSEQAIKARLKRKRVKSDLAEDERQELMIARQIERAQKAQANGEDDDNDANSDDDVGSDDDNEYSGSDEDDDQEEHQEDGKEASKAPAKIAIALQRAAPPPKINPFDDKPKMKFGFEEEEEEVPNKKAKVAGKATDTRRSAIDDLMKEEEKAKERSNRKDYWLCPGIVVKVMSKSLAEKGYYKQKGLVKRVIDKYVGEIEMLESKHVLRVDQDELETVLPQIGGLVRIVNGAYRGSNARLLSVDTERFSAKLQVEKGLYDGKVLKAIEYEDICKVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2EF48</accession>
    <name>F2EF48_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123443460</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374774">
        <property type="protein sequence ID" value="BAK05970.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975772.1">
        <property type="nucleotide sequence ID" value="XM_045119837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EF48"/>
    <dbReference type="SMR" id="F2EF48"/>
    <dbReference type="FunCoup" id="F2EF48">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2EF48"/>
    <dbReference type="PaxDb" id="4513-MLOC_52605.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067870"/>
    </dbReference>
    <dbReference type="GeneID" id="123443460"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0054310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0054310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0054310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0067870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443460"/>
    <dbReference type="eggNOG" id="ENOG502RXVI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088060_0_0_1"/>
    <dbReference type="OMA" id="HNDIYLE"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EF48">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47838">
        <property type="entry name" value="21.7 KDA CLASS VI HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47838:SF1">
        <property type="entry name" value="21.7 KDA CLASS VI HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="77"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK05970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="20639" checksum="453499D8FBF94D93" modified="2011-05-31" version="1">MPRRAIEVRADGAAPKWCMSLLENTFTAFLKSPGADADAKAVFAEGSLFSPYLFGKFFDPADAFPMWEFESDVLLAALRRGARTSVHWSENDCEYCLRADIPGGRKCDVEVSGDGARVVDVSGLWRVPPADGRDWRAGRWWEHGFVRRVELPEDADGGRVEAYFDDGAGSLEIKVPKRNSDAHQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2EHE1</accession>
    <name>F2EHE1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG-Y-related protein A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">High mobility group A protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123452257</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK375568">
        <property type="protein sequence ID" value="BAK06763.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984821.1">
        <property type="nucleotide sequence ID" value="XM_045128886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EHE1"/>
    <dbReference type="SMR" id="F2EHE1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510940"/>
    </dbReference>
    <dbReference type="GeneID" id="123452257"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0424420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0424420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0424420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0510940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452257"/>
    <dbReference type="OrthoDB" id="1110759at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000537">
        <property type="entry name" value="HMG-Y-related protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF162">
        <property type="entry name" value="HMG-Y-RELATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="H15" evidence="9">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK06763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="17510" checksum="F487F088D5FF5551" modified="2011-05-31" version="1">MAAEEVAKPPTLPHYPEMILAAIEALNDKNGSNKSAISKYIEGKYGDLPKEHASLLAAHLLRMKESGQLLFLKNNYFRTDRPNAPPKRGRGRPRKPLDPNAPPKPASPRPRGRPAKPKDPEAEAANPPKKAKTSPPSAVDGSASAKRGRGRPPKARPAEPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL68</accession>
    <name>F2EL68_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123395601</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK376896">
        <property type="protein sequence ID" value="BAK08090.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946562.1">
        <property type="nucleotide sequence ID" value="XM_045090627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EL68"/>
    <dbReference type="SMR" id="F2EL68"/>
    <dbReference type="PaxDb" id="4513-MLOC_64519.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445690"/>
    </dbReference>
    <dbReference type="GeneID" id="123395601"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0369110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0369110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0369110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0445690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395601"/>
    <dbReference type="eggNOG" id="KOG0946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006318_3_0_1"/>
    <dbReference type="OrthoDB" id="198977at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EL68">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000234">
        <property type="entry name" value="Golgin candidate 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006955">
        <property type="entry name" value="Uso1_p115_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04871">
        <property type="entry name" value="Uso1_p115_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="6">
        <location>
            <begin position="382"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Uso1/p115-like vesicle tethering protein C-terminal" evidence="7">
        <location>
            <begin position="800"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="640"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="909"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="779"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="640"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="915"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK08090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="929" mass="101954" checksum="9CBF9A768520E0E1" modified="2011-05-31" version="1">MDSRSINLRGFAGSAGKNIMQGIGGFVFGNEASESKEDSYVERFLDRISNGTMPDDRRSAMTELQSLVAESRSAQMSFGAMGFPVLLNVLKEDREDVELVRGALETLVSALTPIETSQGLKTEVQPASMNSDLLSRETENISLLLSLLTEEDFYVRYYTIQLLTALLTNSLKRLQEAILLIPRGITVLMDMLMDREVIRNEALLLLTYLTRDAEEIQKIVVFEGAFEKLFSIIGEEGFSDGGVVVQDCLELLNNLIRNNASNQMLLKETMGFDPLISILKIRRGSAFNFTQQKTVNLLGALHTVELLLMGGPPGETGKDTSKITNQTALAQRNILDHLLLLGVESQWAPVALRCTALRCIGSLVLRNPQNLDSLASKQVGEEPHVQPALNAILAIILRTSVAQEFVAADYVFKCFCETNPNGQALLASTIAPHPNQGTAIDGASSDMPFGSALLQALVSSDVNGDMEACCRASSVLTHIIKDNLQCKDRVLQIQLETPTPSLGHTEPLLHRIVTCLSFAALAEGENDQSSQLEGSYIQPVILRLLITWLADCANAVNCLLESAVHLNYIIELAANKRFTGCVRGLAAVVLGACVLNNASREKGRDAFAVADTISQKIGLTTYFLRFDELRKSFLHLPSGQQNHKQLSRSSANSMSDFQEIEEEETNKGNQHPVLSEIFDSQFVSLLSKLENDIRECIMDLFSRTKTATAVLPAELEQKNGEVDGEYVKRLKSFVERQCNEMQDLLGRNAILAEDLVRTGGGSTSDSSDKPSSGRERVQIEALRQELEGAARRIEVLKTEKAQIEAEASNQRNLAAKLESDLKSLADAYNSLEQANYCLDAEVKTLRQGGNAPYLDVEAIKAQAKEEAEKESDVELNDLLVCLGQEQSKVEKLSARLAELGEDVDTLLQGIGDDVALPDDDDDEDDDDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0V7G9</accession>
    <name>M0V7G9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123407257</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MET18/MMS19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956286.1">
        <property type="nucleotide sequence ID" value="XM_045100351.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956287.1">
        <property type="nucleotide sequence ID" value="XM_045100352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0V7G9"/>
    <dbReference type="SMR" id="M0V7G9"/>
    <dbReference type="PaxDb" id="4513-MLOC_19714.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740180"/>
    </dbReference>
    <dbReference type="GeneID" id="123407257"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0613950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0613950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0613950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0740180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407257"/>
    <dbReference type="eggNOG" id="KOG1967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="KCSEYVV"/>
    <dbReference type="OrthoDB" id="342900at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0V7G9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039920">
        <property type="entry name" value="MMS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024687">
        <property type="entry name" value="MMS19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029240">
        <property type="entry name" value="MMS19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891">
        <property type="entry name" value="DNA REPAIR/TRANSCRIPTION PROTEIN MET18/MMS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891:SF0">
        <property type="entry name" value="MMS19 NUCLEOTIDE EXCISION REPAIR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12460">
        <property type="entry name" value="MMS19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14500">
        <property type="entry name" value="MMS19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MMS19 N-terminal" evidence="7">
        <location>
            <begin position="48"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="MMS19 C-terminal" evidence="6">
        <location>
            <begin position="703"/>
            <end position="1092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1141" mass="126567" checksum="B5AE58FB98677856" modified="2013-04-03" version="1">MARVPAGDWVPHVEAFVDVSRSPAQHSAGVDALVALVNKDKLTLFDLVSKMDMYLTTTDHIVRARGIMLLGEIMSRISSKWLDVNAITTFSDFFISRLSDWQALRGALVGCLALLHRKLSVGTIMVANVKRLLEAFIHDVQVQSLAAADRKLCFEIFSCILDHYPEAVKTMDDELLYWICQSIDEEKDPECLKLSFHVVEVVMKLFPDPSGLADQFASDFFEILSKYFPVYFTHGAGDNLGVTRDELSRALMHAFCASPYFEPFAIPLLLDKLSSSLPLAKLDSLRYLDNCIQCYGADRMVAHASAIWFKLKEVLFSLSSDQLLSSGSPKDAENNKNEIISEAKHCLKTAVTYIHSSDRDIFINLILLDDDIVNNIHSVTTEENSICSSLQNQKQLQALGSVISILAESSTYLCTRVLQAHFKRLVDILGNSAGFDSQHLNIGNGSSPAAINYGALYLSVQMLSSCREVAVASREGFPAEESWWLILEEKLDQFIQLSGKLLTIDSQTIQSAVRKECVSYAVKGLLILATMPEQCSLVLANAYEDILLMLTSVITSKYEDAHLWRLSLKALTNIGSSAVEFGASKRETVYNRIVVDKIIRLTESCDASMPLNLRLEASFEVGTASVNYMLRVARSLEEAVITNISQVNGSIECVEYVICLIDCYCSRLLPWLFTSGGLNELALSFALRLWDEIGDLVTLDRIRSQGLLDSLMMGMKLLVGVCTEEQQSLIVQKACGIVSSKLSLPVNTMTHHLLSVEELVPAHSVEDTALVCMLSSVIVGLRPRTPLPEMIMIINLFSVFLLNGHIPAAHALASIFNKYVQNSDFSHENKLDKKLDVILGECFSIVLTSSSLKMSHSAAVTSDSANSDSLPGTGSIGSKFDILCGLAWIGKGLLMRGDEKVKEISLFLLKCLVLDQISANIPPHQEEHNDNLSSDASLATAAAGAFHVMMSDSEVCLNKKFHARIKFLYKQRFFSILMPIFLSKIKETPEMTTKLVMYRAFGHIISNAPVSAVITEAHQILLVMVDILAKLSMDIQDKDLVYSLLLVLSGMLMDEKGKECILENMNIIVSVLAQLVSYPHMMVVRETALQCFVAMSSFPHSKVYRMRPQVLQAAIKALDDKKRVVRQEAVRCRQTWQSSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0VAZ0</accession>
    <name>M0VAZ0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123449451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982620.1">
        <property type="nucleotide sequence ID" value="XM_045126685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VAZ0"/>
    <dbReference type="SMR" id="M0VAZ0"/>
    <dbReference type="FunCoup" id="M0VAZ0">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0VAZ0"/>
    <dbReference type="PaxDb" id="4513-MLOC_21553.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400300"/>
    </dbReference>
    <dbReference type="GeneID" id="123449451"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0333110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0333110.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0333110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0400300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449451"/>
    <dbReference type="eggNOG" id="ENOG502QRZD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0VAZ0"/>
    <dbReference type="OMA" id="KCKEPVA"/>
    <dbReference type="OrthoDB" id="593669at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="243"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="462"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="274"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="536" mass="59436" checksum="B29140ADEB4DD95D" modified="2013-04-03" version="1">MSTKASNMSYRYPDNSQIPYYSSSMHVGGNGACYVQQNHEDHQYMSSDGGSQNSDSKSQVIHPQYCTLESSSANCVYAAHSSTSPQCISGSHISLHDSHSDHTYDSPASGITEVPGLGFTNLQELADALFGSDSDAVSSDRSLVIGAAMHQSNWRELLGISSGDLKQVIVACGKAVDENNCHEDLLISELQKMVSVSGEPIQRLGAYMLEGLVARRYSTGHALYKSLKCKEPQPTNSELMSYMHLLYDICPFFKFGYMSANGAIAEAVKGENFIHIIDFQIAQGSQWVTMIQALAARASGPPYLRITGIDDSDSAYARGGGLDIVGRRLCNIAQSCCLPFEFNAVNAASHEVTLEHLDIRKGEAIAVNFAYQLHHTPDESVCIENHRDRILRMVKSLSPRVVTLVEQEANTNTAPFFSRYMETLDYYTAMFEAIDVACPRDDKVRMSTEQHCVARDIVNLIACEGAERVERHEPFGKWRSRFAMAGFRPYPLSALVNNTIRALLNGYNSYYKLEEKDGVIYLGWKNRKLVVSSAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287FED8</accession>
    <name>A0A287FED8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123433639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971397.1">
        <property type="nucleotide sequence ID" value="XM_045115462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287FED8"/>
    <dbReference type="SMR" id="A0A287FED8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0048800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0048800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0048800"/>
    </dbReference>
    <dbReference type="GeneID" id="123433639"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0038050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0038050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0038050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0048800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0048800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0048800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123433639"/>
    <dbReference type="OrthoDB" id="765176at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287FED8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452:SF7">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="865"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="504"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="657"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="923"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="873"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="895"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0048800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="977" mass="108635" checksum="F695A644484F6D47" modified="2017-11-22" version="1">MFKSGRWRGAGKAKAVFKLQFHATQVPELGWESMMVVVTPQDVGRPTARTERAEVADGACRWPAPIFEATKLPSGKATAGDKIYQFLVYEAGSSKAALLGEATVNMAEYAEAFKPSAVTLPLKGSPAPGALLHVTIQRVVGGAGGGYGDDGSESGDTAKSSPRRTLQSQLSRCEDEEAEKARSLAADSMSPVHDGLVISKPPGMRFPLRRNMPASVEPPGHLHNANGFDAVSLSGSDGSSGRFTPKTSANMHGAFLQDATNVLSPFANNAPSRNPLSSGDWSGSSAPDASTDGSTSNSGETGLRGAEDDVEKLRSEIGTLTRKLDVSDMELQTLRKQIVKESRRGQDLSKEMSSLRDERDALRRECEGLRGMKKTIHDANGSGKRLSDGEDPWSQVEELKQELGHEKNLNADLRLQLQKMQESNSELLLAVKDLDEMLEQKNRDMSILQEEIVEDPQEAEYEHALSNVHSAGHKMDMSETSSCQEKEDELMLDALAKKSDGIASSELQEKILELSDEIELYKKDREDLEMQMEQLALDYEILKQENHDISSRLEQTQLREQLRMQYECSAHLSIISDLEANVENLENELQEQSQRLEADIAEVLAAKVEQEQRAIKAEESLRKARWNNATTAGRLQEEFKSLSSQVSSAFSANERLLVQARKEAAELQLQKSQLEELLQKAHEDTASAQEQHRVKIQQLLTLVDFKSNEIDRLVVELKRKSDEFENQKRSDESKLNDLSEEIEQLKAKIDKLSDERDKLVERNEQKDMELAANGEKDMILQDKTGEITLLNKELALLKDQMQAYLEELNTLKRSKSERDETIGKLQITIGSLKLQYDNMKNSLSTKESEKSNLASQVLKLRRALESREGANENGVTSDTTDNQHANSKRIKHDTVSTGNGDATPSANGHSNGQDTRGAADHQSSKELEALKELNKAMQQELNELHERYSEISLKFAEVEGERQQLVMTVRTLKNSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287VMP5</accession>
    <name>A0A287VMP5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123408937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957897.1">
        <property type="nucleotide sequence ID" value="XM_045101962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287VMP5"/>
    <dbReference type="SMR" id="A0A287VMP5"/>
    <dbReference type="STRING" id="112509.A0A287VMP5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650550"/>
    </dbReference>
    <dbReference type="GeneID" id="123408937"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0539870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0539870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0539870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408937"/>
    <dbReference type="InParanoid" id="A0A287VMP5"/>
    <dbReference type="OMA" id="CNPRSIG"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287VMP5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF78">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="38636" checksum="22188167A236874A" modified="2017-11-22" version="1">MAVRYLLLLAIFLLPTQPTTQAGKKQPAVPAVMVFGDSLVDVGNNNYIFTIAKANFPPYGRDFKDHVATGRFCNGKLLIDFIAEKVGFNSSPPAYLSPQASGWNLLLGANFASATSGYNDHGTLVNAISFSQQLKYFEEYQGKLAVVAGGSHAHSIISGSLYIISAGSCDFVFTYYINPFLYMTQSAEQFSDRLIGIFNNSVTQLYGMGARRIGVFSLPPFGCFPVAITLYGHGRNECVLRLNNDIQYHNMKLRATVDSLRKKYHDLKIVVLDIYTPLYNLATSPVSQGFTEAKRACCGTGTVEASILCNPLLPGTCPNARTYVFWDVWHPSEAANKVIVDSFLDDIDILVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W5F0</accession>
    <name>A0A8I6W5F0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="3">1.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044969329.1">
        <property type="nucleotide sequence ID" value="XM_045113394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W5F0"/>
    <dbReference type="SMR" id="A0A8I6W5F0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0037670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0037670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0037670"/>
    </dbReference>
    <dbReference type="GeneID" id="123429346"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0029160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0029160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0029160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0037670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0037670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0037670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429346"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF3">
        <property type="entry name" value="NAD(P)H DEHYDROGENASE (QUINONE) FQR1-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="7">
        <location>
            <begin position="54"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0037670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="26356" checksum="FAFDA8495E80A054" modified="2022-05-25" version="1">MGKGGGCVPSKKRQPPATTVATAPSSSSATAVPRDAPEEAQDAPTGESSQTLRLYIVFYSMYGHVEALARHAAAGAGAVEGVEAVLRRVPETLSAEVLEKMQAPSKDPAVPVISAPADLEEADGVLFGFPTRYGAMAAQMKAFFDSTGSLWEKQRLAGKPAGFFVSTGTQGGGQETTAWTAITQLVHHGMLFVPIGYTFGSGMFNMDDIRGGSPYGAGVFAGDGSRQPSEAELALAEHQGKYMAAFVKKLGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W7M7</accession>
    <name>A0A8I6W7M7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">rRNA methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123416973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044962769.1">
        <property type="nucleotide sequence ID" value="XM_045106834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W7M7"/>
    <dbReference type="SMR" id="A0A8I6W7M7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079400"/>
    </dbReference>
    <dbReference type="GeneID" id="123416973"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0064690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064690.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0079400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123416973"/>
    <dbReference type="OMA" id="QDQETKP"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF26">
        <property type="entry name" value="SAM-DEPENDENT MTASE RSMB_NOP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="9">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="10">
        <location>
            <begin position="237"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="384"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="53232" checksum="E8292958FEC58DBA" modified="2022-05-25" version="1">MGKAAKGKQRRDEYYHLAKEQGYRSRAAFKLQQLDDRFRFLPAARAVLDLCAAPGGWVQVAVARAPAGAFVVGVDLVPIRPVRGAHSLTEDITITRCRSAVRRLMDSRGVAVFDVVLHDGSPNVGGAWAQEATAQSALVIDAVRLATAFLAPKGAFITKVFRSQDYSAIMFCLKQLFDKVEVTKPRASRGTSAEIYIICLKYKAPAKIQPELLDIKHLFSVDPEKSTPTDVLDTNKNAKKRTRGGYAAGVTVLEKVGLASDFIWSEAQKPREFLGSFTKISFDDPASLPIENHELTTDEVKCLCEDLQVLDKNSFKHILKWRTRLRNALPSSSQVTPKADGTATNTKVKGDVQLLQEMEEMGSDEEHQLDGMLDEAYERYVTKKGEEVKQEHKRAKRIDPDADAGLLEGGEDDGGNVGTDQGSNKDQDQETNPLLSCLDTEEPAEEQDVKVVVESGYVHRSRKKGKGGKGGAKGGKGMKGSAGQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W9E0</accession>
    <name>A0A8I6W9E0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123424938</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044964557.1">
        <property type="nucleotide sequence ID" value="XM_045108622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W9E0"/>
    <dbReference type="SMR" id="A0A8I6W9E0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0100990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0100990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0100990"/>
    </dbReference>
    <dbReference type="GeneID" id="123424938"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0100990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0100990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0100990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424938"/>
    <dbReference type="OrthoDB" id="673077at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1075">
        <property type="entry name" value="OS06G0644300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="177"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="418"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0100990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="55432" checksum="5CCBD4D4D7F26DE1" modified="2022-05-25" version="1">MAQALVQRACEKLRSAIGDEAVARSNFTGDLREMLEALEHIQPLLDRAEMHLWRDFFPSRWLQLALAAAYKTMDMVDELQGSRPQAAATMTRRMLPRLAIKKNAMAIQVQETKILMMFVQGHSGLLSEELAQSTILLEKINKEREIVPAFEEAMVLGRGSDKERIITALPSTQPNIMQEHITILPIFGLAGSGKTTLAQMVFNDNHSLQVYDFRVWVHVSPQFDFHAIGKSIISQVSARGQEETNHSSSDMEGMQNIMKRLHELLNGKKVLLVLDDLWEEDPIRLQLLKSMLTFLGDRMDVIVTTCNQAIARKICTIEPYRLNPLSDEACWEIIKKLIRFEGGEEELEKIGREIASKCSGVPSAAREYAAMLRSSRDAMTWKNKMKEDILFYALPAFKLSYMRMPPELRLCFAYCCQIFPSGHIIVKDDLVHHWIALHLIEPSEILSAAQIAEEHITRLQDMSFLQTAQLNHVSNCLLLCCTKFTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WL34</accession>
    <name>A0A8I6WL34_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123428430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968572.1">
        <property type="nucleotide sequence ID" value="XM_045112637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WL34"/>
    <dbReference type="SMR" id="A0A8I6WL34"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0212690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0212690"/>
    </dbReference>
    <dbReference type="GeneID" id="123428430"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0176870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0176870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0176870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0212690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0212690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0212690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428430"/>
    <dbReference type="OrthoDB" id="607736at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675:SF16">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="ARSENITE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="867" mass="97782" checksum="FF3F0A921628AA8F" modified="2022-05-25" version="1">MRVAVVGGGVSGLAAAHELLAASGGGDVRVTLYEQEESLGGHARTVAVEDGAGGCVNLDLGFMSFNQVTYPHMTEWLEGLGVEMERSDMSFSVSKQPEGNGRGCEWGNGNGISSLFAQKSNILKTSFWRMLGEILKFKNDALAYLEYHQDNPDLYCNETLGQFIQSHGYSLLFQEAYLIPVCAGMWSSPSQGVLTLSAFFVLSFFRNHDLLQLFRYPQLPTVKARSHSFVEKVKGELESLGCRMKTSCRVKSVSSLDGTSGYRVLENDGSEETYDSVIFGVHAPNALKVLGAEATHDELRILGACRHVQRDIYLHCDQNLMPQNSFAWSAWNFMGITSSVFSVTYWLNHIQKIESVRPFLVTLNPPCVPDRVLLKWSTSLLVPSVAATKAYLQLDQIQGKRGIWFCGAYQGHSFHEDGLKDGKAAAQGLLGKKCELLLNRKQIIPSWTEAGARLMVTRFFNLYVSIGNLIFVEEGGSVFSFGKACDKCRIKSVIRVHEPLFYWKVAIEGNLGLAEAYINGCFSFLDKREGLLNLFLILIANRDERRSNRIARKGGRWTPLHVIARLAHARYILREVSRKNTVTQTRRNISLHYDLSNDFFSLFLDKSMTYSCAVFKMENESLEVAQQRKLSLLINKAKIKRGDHVLDIGSGWGSLAIQAVKQTGCKYTGVTLSAEQHKYAERKVREAGLEDHITFLLCDYRQIPPCKYDAIISCGMIEHVGHEYMDEFFACCESYLAEDGILVLQFISAPEERYEQYRRRTDFIKEYIFPGGCLPSLARVMSAMTTSSRFSMEHVENIGPNYYTTLMHWRDNFIANKDKILALGFDDKFMRIWEYYLIFSAACFKLRALGDYQVVFSRPGNRRLGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLD1</accession>
    <name>A0A8I6WLD1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123431269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971026.1">
        <property type="nucleotide sequence ID" value="XM_045115091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLD1"/>
    <dbReference type="SMR" id="A0A8I6WLD1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213620"/>
    </dbReference>
    <dbReference type="GeneID" id="123431269"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0177660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0177660.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0177660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431269"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000501">
        <property type="entry name" value="Os04g0682400 protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF3">
        <property type="entry name" value="31 KDA RIBONUCLEOPROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="267"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="371"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="473"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="574"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="668"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="762"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="889"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="900"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="994" mass="108368" checksum="E52860FC67FD4CAB" modified="2022-05-25" version="1">MGCKSDLTDQLQKDGMSVVTADYEEGSEEEEPFEREFYDDDDDDYEEGSEEEEPFEREFYDDEDGDDDDCEPKTQAVDSSEVSLADVSEEEPCDVAAPLEDEDASYEEPFVSELCYEEEDSCEQDSYYAGPHTDSYRKKLYEVEPCDDQVAHEYEFVKKKSSTVQTINKEQNKKVVLKRALRKGSSNELKAVPVIDDMETKPSKKRLSVRFATDVSCYTYSTESFGAAKLEKRKAQFDDQDSHLRKRQEHIVSSLKDGGKLKEVDETNLYVGNLPASVSSHKLIELFLPFGRIVRSKVADDRITGISQGYGFVKYASARSAAAATERMNGRLIDGKALEVRVAGPPTSVSNPCKQSVSETCSLPSKEIDASNLYVSNLPLSMDTLKLLDHFLPFGKVTYIKVPRDHTTGLSKGYGHVKYADSRHAAQAIIHLNGLLVEGKRIEVRLSDISPTLSNSAVGSHTNSRTIKEIDMANLYVCNIPASINTNKLIDLFLPFGKITQARVAADQEGAHSAKGYGFVKFADSQCAAEAIALMNGALVEGETLNVRVAGLSSSASSSAVQGSSIKSPEINKSRLYITNLPQTMNDNKLIELFLPFGQISKVVINPEYSLVYYADVASAMTAAERMDGYLIGGKRLVVRGSEYCQPNAAEQALSQPAGKPTKEIDMANVYVGSIPPTVTCDQLVELFRPFGQIVQSRLFHGYGMVRYNHPSSATAAIDHMDGYQIGGSSLVVRVAGLPNPGDYLTVRAPVSGNEQRQIDMTNLYVCHLPLHVTAEKLIEIFLPCGQITQAKVVVDWHTGGSRGFGFVKFGDAYGAAVALTHMNGCPLEGHILGVRIAGVHPSDMDSYMTRLYSQFTLPDPSMMAVGIPTSSYWPYYCAESAYVEGQGADTASQTSQEESVSASSFAEKGSSSVSSHVTDSSQQHSSAGWAGPPGFNPHTISSQPPSTGRAGPPSFNPHATAKKDSATARKPSQPCSKVHLAQSGGSQKRRSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMH5</accession>
    <name>A0A8I6WMH5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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        <source>
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</entry>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    </dbReference>
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            <end position="271"/>
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</entry>
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    <accession>A0A8I6WTK3</accession>
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        <recommendedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
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        <name evidence="7" type="primary">LOC123425887</name>
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    <organism evidence="7 8">
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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            <strain evidence="7">subsp. vulgare</strain>
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        <location>
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            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
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            <end position="166"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0140950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1355" mass="152564" checksum="39CDDE173F3E2F52" modified="2022-05-25" version="1">MNRGELTDPPFRDVSNLGTPKPNPRSPSPLFFTASKTPLPAPIPTTLARSCRRPHASAATPTPIGRRKALDGAATPTPLARRLRVLELDQSRSARRAESGRERALRSFAASASSWLSLLLRDPSACGCSPAAAATATRVTVAGKRDTLDGERARGSSPKRHRGRDRCYREKRRAMTPAMVAVLRESLREVCSLDDVKERMGKYMSTEACEEVLIMMCQICKNIDERRLKMKAHCPIVTDLRLKENATRIFLCYNPEWLRVGLHIVLGGDCLLQSGSWKRDKEVHFLKLLLEKQLFGQNGTGYTETLGNIILKRMFLLVAALDRAKMERTLPLKFGIDSLDGGSPPLFCHRANIKSSQQIIQKSLAEAMHGEGDLLVHLSIMGYKLNYQQLALSEYDFTIGNLFEDLHDGIILCRVVQLLLSDASIILKVIAPSDTHKKKLQNCTTAIQYIKQAGVPISDADGVTISAEDIANGDKELILSLLWNMFIHMQLPLVVNKTSLARELSRLKALAVEQPISVTKSHMGLLYDWIQVVCAKCGLSVESSQIDRRALNYFINHYLNISIHTCPLKKTFSDCRKELFSCHEQTATAISSCPSSKIEDLGDFLQDFPASGILANDVLFDEKGAIILIAFLCSHLTNDKRLEQLRNSISGRLDCQRLENRVSARLRSPGKNDVKYQSPQTEKAATIIQAQIRRIIAKNRYHKLKRSISILQGAIRAWSFVNMTRKSSCLTAASPTHARAYGSYSRYLIFILERHRFVRMRRSTIVIQQAVRIWIGERKRSENIEPFESHEFLETTASPKTDCIEMCDGEHETTPCKDLGILVTSAAPQCLDESNHIDTVTIVQLHVKNSNYVTSPSPHQSIIKSGSVDSVSHQPCEIETDSIASATRLACEADVDCGSNISCGASFQHEHPVSAQLDFSLRKDIMAVQKIQFAYRRFVHNRSSRIYAATKIQSHWRGFTMRMCFTKQVEAITVIQSVARHNLCSWAFQRFHNAALDIQRIARGGFARKRLLGSSLQTYTSLVSLDQSQHKRSHQSTELKIVLYSTLRLQRWWRKVLLHQSIKLSAISIQSSVRGWLARKQVKRITCCVHVIQRWWRNVMFLESRKRAATTIQSHVRGWIARQSAIRKKKSITIIQSFVKAYHVRKASKEEVVYIRSRIQKASAQVDDGMRLINRLIAALSQITGCRSISSIRQTCTTLSIATELSEKCCETLVDAGAVDILLKQIPRLNRGIPDQEVLKQVLYTLRNIARFPNVRPVLANNPQLVNTIFQELLRNKTDMFFIACGILMKLCESEEGRGFAGALRHHIRGLGSMVRGLEKKVELDKRNGRTGARKEDNLRRLGEAAALYHLLTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WU59</accession>
    <name>A0A8I6WU59_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123430472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970279.1">
        <property type="nucleotide sequence ID" value="XM_045114344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WU59"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178420"/>
    </dbReference>
    <dbReference type="GeneID" id="123430472"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0147590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0147590.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0147590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0178420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178420.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430472"/>
    <dbReference type="OMA" id="HRTRKFF"/>
    <dbReference type="OrthoDB" id="1284989at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34124">
        <property type="entry name" value="F16B3.27 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34124:SF18">
        <property type="entry name" value="OS04G0496300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="20221" checksum="98B3AE579DEA1CB4" modified="2022-05-25" version="1">MGLSVIAPPAGDSASPAHRRARRAFLVSNYMILGAASGCGFLTLSLRLVPSVNGFLLILLHALTVAAAVAGCAVIAAPDPPRGRVYTAHMSGTVVVSILQGAAAVLAFSRTSEFLVDGLRSYVREEDGAVILRMIGGLGIAIFCLEWVALALAFVLRYYAYVDRECGGNPMRRSAKVGGEDGAGNWPWPFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WWB3</accession>
    <name>A0A8I6WWB3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123442146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974163.1">
        <property type="nucleotide sequence ID" value="XM_045118228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WWB3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317650"/>
    </dbReference>
    <dbReference type="GeneID" id="123442146"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0265190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0265190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0265190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0317650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442146"/>
    <dbReference type="OrthoDB" id="637689at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="1">
        <location>
            <begin position="107"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="43377" checksum="9A6FE4EE666059A5" modified="2022-05-25" version="1">MGKAARSGALHGLLEDIVVWEVLIRLPPKALLRCRAVCRAWRSATSTREFLLAHHAHQPVLPLLDNGSYLNLDDPIDICCFDYRPGLAAPDQLQSVAFLDLGDDISYCQPVASCDGLLIFFIDDAELFICNPATREYAPIPSPPNQHWTLLGMYPHTLTDEYRLLLYSCERNPGNHEPAHACQVLSLGSGQPPRHIGWPDAITTAILMELRPPVLFRGNLHWIPSDDDSESTMIMVFDTTSELFRQMRTPVVNDCANLFEIDGKLGMAILNVATTIEIWMLQDYEKEIWIINCRIELPVAEIKVRCDNYDGRLDFHVVLVPGDGELLVLVQLAEWLFQLGTDGKLVTALHRKEVDATYLQLKQTLVPHTFFHTLEGYVVNAPPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X537</accession>
    <name>A0A8I6X537_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CG-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123449676</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAMTA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982905.1">
        <property type="nucleotide sequence ID" value="XM_045126970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X537"/>
    <dbReference type="SMR" id="A0A8I6X537"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406930"/>
    </dbReference>
    <dbReference type="GeneID" id="123449676"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0338780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0338780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0338780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0406930.1">
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        <property type="term" value="P:response to cold"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR CAMTA"/>
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        <property type="entry name" value="Ank_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CG-1" evidence="16">
        <location>
            <begin position="15"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="704"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008267"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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            <dbReference type="ARBA" id="ARBA00022860"/>
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            <dbReference type="ARBA" id="ARBA00023043"/>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    <sequence length="1060" mass="118165" checksum="2C3A8C4895B80455" modified="2022-05-25" version="1">MAEGRRYGIAPQLDMEQILKEAQTRWLRPTEICEILKNYRNFRIAPEPPNMPASGSLFLFDRKVLRFFRKDGHNWRKKKDGKTVKEAHERLKSGSIDVLHCYYAHGEENINFQRRSYWMLEEDYMHIVLVHYLEVKAGKSSSRTRGHDSMLQGAYVDSPLSMPSQTTDGESSLSGQASEYEAESDIYSGGAGYHSISRMQQHENGGGSVIDGSVVSSYGPASSVGNHQGLQATAPNTGFYSHYQNNLPVIHNESNLGITFNGPSTQFDLSSWNEMTKLDKGTHQLPPYQSHVPSEQPPFTEGPGIVSFTFDEVYNNGLDDGHANTDKEPLWQLPSANDGTTTEFQLPSATDGITTEFQLPSATDGTFGTVDSFEQNSKLLEEAINFPVLKTQSSNLSDILKDSFKKSDSFTRWMSKELAEVDDSQVKSSSGLYWNSEDADNIIGASGRDQLDQFTLDPMVAQDQLFSITDFFPSWTYAGSKTRVLVTGRFLTSDEVIKLKWSCMFGEVEVPAEILVDGTLRCYSPSHKPGRVPFYVTCSNRLACSEVREFEYRPSDSQYMDAPSPHGATNKIYLQARLDELLSLGQDEQNEFQAALSNPTKELIDLNKKITSLMTNNDQWSELLKFADDNQLAPDDRQDQFVESGIKEKLHVWLLHKAGGGGKGPSVLDEEGQGVLHLAAALGYDWAIRPTITAGVSINFRDVHGWTALHWAAFCGRERTVVALIALGAAPGALTDPRPDFPSGRTPADLASFNGHKGISGFLAEFSLTSHLQTLNLKEAMGSNASEISGLPGIGDVTGRIASPSAGQGLQAGSMGDSLGAVRNAAQAAARIYQVFRVQSFQRKQAVQYEDDNGVISDERAMSLLSYKPSKPGQFDPMHAAATRIQNKFRGWKGRKEFLLIRQRIVKIQAHVRGHQVRKHYRKIIWSVGIVEKVILRWRRRGAGLRGFRSTEGATESTTSSSSVDVIPVKPAEDDYNFLQEGRKQTEERLQRALARVKSMVQYPDARDQYQRILTVVTKMQESQPVEESMLEESPEMDEGFLMSEFKELWDDDMPLPGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X928</accession>
    <name>A0A8I6X928_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123447267</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979792.1">
        <property type="nucleotide sequence ID" value="XM_045123857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X928"/>
    <dbReference type="SMR" id="A0A8I6X928"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335500"/>
    </dbReference>
    <dbReference type="GeneID" id="123447267"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0279770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0279770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0279770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447267"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="23307" checksum="FB58D0C9D49C04DC" modified="2022-05-25" version="1">MECVIGVVGRDFAVVAADTSAVQSILVHKTDEDKIMLLDSHKLMGASGEPGDRVQFTEFIQKNLHLYQFRNNMPLSTAATANFTRGELATALRKNPYSVNIILAGFDKDAGASMYYIDYIATLHKIDKGAFGYGSYFCLSLMDKLYHPDMTVEEAVDLVDKCIKEIRLRLVVAPQNFAIKIVDKDGARDYARREIGGDSPAAATTAATVATTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XA31</accession>
    <name>A0A8I6XA31_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123444443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044977086.1">
        <property type="nucleotide sequence ID" value="XM_045121151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XA31"/>
    <dbReference type="SMR" id="A0A8I6XA31"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289910"/>
    </dbReference>
    <dbReference type="GeneID" id="123444443"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0240780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0240780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0240780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0289910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444443"/>
    <dbReference type="OrthoDB" id="191529at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000212">
        <property type="term" value="P:meiotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000056">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000490">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF171">
        <property type="entry name" value="KATANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="125"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="373" mass="41914" checksum="D775186A81B6934F" modified="2022-05-25" version="1">MDLRAFFGGKKQEPEQNGHANGGVKKRTADLAIYEQYEQQARQPEMRAAAIRDGNADVIQKPLLPQFESAEMRNLAETLLRDIIRGSPDVQWESIKGLETAKRLLKEAVVMPIKYPKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKTKDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEAEARHSMFKELLPAMTSNLEVPYDILVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVLEASDELVPEEELPEVGPLKPDDVELALSNTRPSAHLQAHRYEKFNQDYGSHILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAE2</accession>
    <name>A0A8I6XAE2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>BOP clade</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAE2"/>
    <dbReference type="SMR" id="A0A8I6XAE2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="B3"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="193" mass="22183" checksum="0ABB567934D236E7" modified="2022-05-25" version="1">MEFFTIILEKSSSRLVLPNNFVKMLDGHRPQNMKLRQAGNGLRKLWDVEVVFDTDGSMYLDRGWKQFVRAYDLRIGYFLVFRYDGNATFAVKVFNTTICRRCYPDDDDASNGSRSSEYCDRCYVYGSSDSDSKSNNDSGYSKSSSDDGLAMDVPQLGDRAMPIVVEEYIPQPGLGLRRSKRIRMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDQ4</accession>
    <name>A0A8I6XDQ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123440976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973448.1">
        <property type="nucleotide sequence ID" value="XM_045117513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDQ4"/>
    <dbReference type="SMR" id="A0A8I6XDQ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321680"/>
    </dbReference>
    <dbReference type="GeneID" id="123440976"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440976"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905761">
        <property type="term" value="F:SCF ubiquitin ligase complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000022">
        <property type="entry name" value="F-box protein FBW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF70">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36240" checksum="3C86650C7E1829E3" modified="2022-05-25" version="1">MEECSKNIYWEDLQRDALGIIFCKLSLQEILRVVPGVCKPWSEVVSGSYCWQDIDIQEWSQQSTPDQIISMVHMLLARSAGSCRRLSVSRLPNDSLFAFIADHAKSLETLEIPRSEISDSIVEDVAQRLSKVTFLDVSGCTKIGARALEAFGKNSKCLVRLRRVMHPMDLVGKVCHNDEAHAIACNMPKLCNLEMGYMLITTKAVFEIASKCHDLKLLDLRCCWDVDDNLLREKFAGLKILCPRVDDCYENNFWEECSDDSYDDSIYSWEQFMDDDYFAMGGDGEAIWEDDHTLEGQEVIFYGGVFGEGFAGFDWPDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG91</accession>
    <name>A0A8I6XG91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123451199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984141.1">
        <property type="nucleotide sequence ID" value="XM_045128206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG91"/>
    <dbReference type="SMR" id="A0A8I6XG91"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345530"/>
    </dbReference>
    <dbReference type="GeneID" id="123451199"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0288460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0288460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0288460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451199"/>
    <dbReference type="OrthoDB" id="618087at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338229" description="Dirigent protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="185" mass="19632" checksum="4DF282AB4379D2A8" modified="2022-05-25" version="1">MDCGKLVAFLLLCSAVLRGTTAAYNTCGAASSIIHLHFYMDDFYTGPNPTALRVVSGRSLSPDNGTAPSPRQFGDIVALNDPLTEGPDRGSARVGTAQGFAVRVSEGGVVSDLNLHLFLEAGEYSGSSVVVNGRVDLDLATRESVVVGGTGQFRFARGYMLSRDYEYDLANGGVVELDVYVQVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGM9</accession>
    <name>A0A8I6XGM9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123395763</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGM9"/>
    <dbReference type="SMR" id="A0A8I6XGM9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0405670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0405670.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0405670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0489610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000035">
        <property type="entry name" value="Cationic amino acid transporter 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF53">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
        <location>
            <begin position="625"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="724" mass="76848" checksum="4265AF8612B13B9C" modified="2022-05-25" version="1">MESVRAINAMFDDVASSVCSCVHIPVLLCFLAIAGADFLLDTGDKPKIGWTGSPQRWRVSDQRKKTTDLSCPESCTSRVHGLEQLVSCICTDKECRAPRCSCALLDPKLQAMESQEKPAVAPRSYWRWSKDDFFPEPSFASWGAYRGALAATPARLRDRLLAGRSTDAAELGAMRRRSENEMRRCLTWWDLTWLGFGCHLGAGIFVLTGQEARDHAGPAVVLSYAVAGISAMLSVLIYAEFAVEIPVAGGSFAYLRVELGDVAAFVAAANLILETVIGTAAVARAWTSYLASLFNMPVSALRVHVPSLGDGYNELDPIASAVILATAIMAMLSTKGSSRFNWVASTVHLLVIAFIIVAGFIHAKPSNLTPFVPYGVPGVFRSAAVVYFAYGGFDSIANMAEETKNPSRDIPLGLIGSMSVITGIYCTMALALTMMQPYTAIDRSAAYSVAFGALGMRWMQYVVAVGALKGMTTVLLVGALGNARYATHIARSHIIPPVFALVHPKTGMPVHATALITAASACVAFFSSLDVLASLLSISTLFIFVMIASALLVRRYHARGVTSQAHARRLAALVLGVIGSSAGIAACWGAAPERWEGYAALVPVWAAATLGIQLLVPVARTPRQWGVPLGPWLPSLSIATNIFLMGSLGAQAFVRFGVCTAVMLLYYVLVGLHATYDVAHEEVEEQGVGYGDHAAADEKAAAAAAAGAEAATDVEKSGVGAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGQ7</accession>
    <name>A0A8I6XGQ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Floricaula/leafy-like transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123427761</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FLO/LFY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967815.1">
        <property type="nucleotide sequence ID" value="XM_045111880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGQ7"/>
    <dbReference type="SMR" id="A0A8I6XGQ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194240"/>
    </dbReference>
    <dbReference type="GeneID" id="123427761"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0161110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0161110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0161110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427761"/>
    <dbReference type="OrthoDB" id="1875842at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010074">
        <property type="term" value="P:maintenance of meristem identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905613">
        <property type="term" value="P:regulation of developmental vegetative growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4180.10">
        <property type="entry name" value="Protein LEAFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035209">
        <property type="entry name" value="FLO/LFY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002910">
        <property type="entry name" value="FLO_LFY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038276">
        <property type="entry name" value="Floricaula/leafy_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035079">
        <property type="entry name" value="LFY_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36079">
        <property type="entry name" value="PROTEIN LEAFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36079:SF1">
        <property type="entry name" value="PROTEIN LEAFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17538">
        <property type="entry name" value="C_LFY_FLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01698">
        <property type="entry name" value="SAM_LFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Floricaula/Leafy protein SAM" evidence="11">
        <location>
            <begin position="46"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Floricaula/leafy DNA-binding C-terminal" evidence="12">
        <location>
            <begin position="213"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="42659" checksum="AFA4ABE263B23DC7" modified="2022-05-25" version="1">MDPNDVFLAAHPFRWDLGSPAPAAVPPPPPPPPPPPALPPANAPRELEDLVVGYGVRASTVARISELGFTATTLLVMTERELDDMTAALAGLFRWDLLIGERFGLRAALRAERGRLMSPGCRHHGYQSGSTVDGAASQEVLSNERDGAASGGIGEDDAMRMMASGKKQKNGSAGRKAKKARRKKVNDLRLGMQGDEHEDGGGGLSESTESSAGGGVGGERQREHPFVVTEPGEVARAKKNGLDYLFHLYEECRVLLLQVQSMAKLHGQKAPTKVTNQVFRYASKVGASYINKPKMRHYVHCYALHCLDEDASDALRRAYKARGENVGAWRQACYAPLVDIAARHGFDIDAVFAAHPRLAAWYVPTRLRQLCHQARSSQDAAAAAAHAAAMPPAMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHU7</accession>
    <name>A0A8I6XHU7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHU7"/>
    <dbReference type="SMR" id="A0A8I6XHU7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0429280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0429280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0429280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0429280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0429280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15726" checksum="4D5BB7B56026B23B" modified="2022-05-25" version="1">MVLVSASAGPRHVYDSDVEVCEPEIPTHIKIRVEKLNLVEKQNKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAVRLKMDAMRLKMDEMHLKIRNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHZ3</accession>
    <name>A0A8I6XHZ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHZ3"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405970.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MAIN-like"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="19331" checksum="1D2D43691F412E3D" modified="2022-05-25" version="1">MCLRDQHLWRVRCPMICFYAMEWHLPHRVSKQFGVQQRTPPDYVETSVKLHKTKRQSNKTVINWEEHHITWVDMWNAQRQHRVENDETPDTDEAAYLRHLEWMRTEYRVIHKVAWTRSDCLDLLPSEVADAAFNNSIRETVGAHLDYGPLHDRVVRPSFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKI2</accession>
    <name>A0A8I6XKI2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anthocyanin 5-aromatic acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123431112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970882.1">
        <property type="nucleotide sequence ID" value="XM_045114947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKI2"/>
    <dbReference type="SMR" id="A0A8I6XKI2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206740"/>
    </dbReference>
    <dbReference type="GeneID" id="123431112"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0171780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0171780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0171780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0206740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0206740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0206740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431112"/>
    <dbReference type="OMA" id="ICCRTAG"/>
    <dbReference type="OrthoDB" id="667639at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
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    </dbReference>
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        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CoA-dependent acyltransferases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0206740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="48337" checksum="5DEC9B2050EFB85F" modified="2022-05-25" version="1">MSQVRIIDASYVHVPEAAVRPPGPIKLTAMEALWVIIPVLQHVLLYDYEAADDMPPFDAIVQSLRSSLAATLRSFAPLAGRLVHLEETGDVGVCCSASDGVRFVVAECDADIRRLAGDEEHDLPVLEGLVPEVDMSHLPVPVLAVQATRFEGGVAVGVTVHHGVVDGRALWTFVEAWATACRGETPATTPCFDRSLVKLPGGEELARSVLRKLAPNMPSVAIPSSLLEDRKRFTRRTFTLDAGDIQRLKQRIVHLSESHGAPLPRPPSTFTVVVALVWTCFARCKPFSSEDDAQLFFFADTRERLDPPVDAGYIGACLTGCLSKLPVPELRGDRALAAAAATVQDGINKVKEDPVAGWNFFELMTDVSMDRIMNISGSSGFRAYDVADFGWGKPRRTEPIRMNHDGQVALMRSRDGRGVQVSVALLQPGHMDAFKSSFFELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLG6</accession>
    <name>A0A8I6XLG6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLG6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="179" mass="19713" checksum="BFB35FC16978F5F3" modified="2022-05-25" version="1">MYMGTEGVPHAEVFQHVSSVLEDYGFCPTGDPMHAINLRPYDCRKSNRQSGKGLTDANMAVMESANLDAAEQKAVRKKVGRGWDPTYTKRSFFPFWKSVLKARFPLKVYPVYASSDRMSGLNGQVRVSPSNSTGNGSTPTPNHALGPLQRISPLTSNNQSRRCFVTAASGAFRLLKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMW1</accession>
    <name>A0A8I6XMW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin 6/10/61 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123450716</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
            <location evidence="8">trans-Golgi network membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMW1"/>
    <dbReference type="SMR" id="A0A8I6XMW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0331230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0331230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0331230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0398090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21442">
        <property type="entry name" value="SNARE_NTD_STX6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000004">
        <property type="entry name" value="Syntaxin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34949:SF6">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34949">
        <property type="entry name" value="OS05G0443700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Syntaxin 6/10/61 N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="356" mass="39135" checksum="F9C7978A3CBB4694" modified="2022-05-25" version="1">MATSFDRWEKDPFFLAAEEVQESADRMESVYKIWVQERSGGDSALAGEVGAVELRRELHTALGTAKWQLDELERAIRSNDEVVSAGKDTRALHSNFVAAIAYRILEVENNLNASNVAEGRGTLSWIHLDESERDDLAAFLSASPPQHQDKVVTIPSAGDIQVGSNPARVRKNISADSSNDSSGSADLSSARAKEDAHRGHRRSVSASADIGSSPMAFPNEREGAAEQSSLGPHRAPLLNIVKACGLPSALKPKPAIKYKKGAVRWAHADKHDVEEGLPLTNPQLSQGLDGYSERSMSYLNTCDGVTYIKKLYGWFGALRKKLQRSQYQIRYGRPVQLTVFALAVLIIFVFVFKTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNZ0</accession>
    <name>A0A8I6XNZ0_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123449577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982787.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36373:SF1">
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0404030.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="43260" checksum="84D6868B54667D30" modified="2022-05-25" version="1">MESMEPMDIDWNRVVSRFVRDDTYEGIEAPHWADLADPEAGNAAVDDDAWFCRPDCKHPKTADDFLRQTPSPKARLLRSMSAMMPFGERDANNNLKRRGAGGTAAFASPTKPKPPPKKRFQDDAENQDPALTTPPPAATRPPFGAQRWAKNAKEAIKSSAEKRPDNAEKEALLGRNPAPRQLKSTLSARNLFSGKDILGQISDFYNELKRMAGGGGSQQPVSEDMEEMSPINSSVVVEKVDCSSGGGARVLPDAVKKVARQETAQKSPSPMKGKKAGLKVEAGRQRSPSVLKEVKGQRSPSVLKEVKATPPTPQRFPSPSVNRVKSVKAAGATSSSPLKKTLKDKMTPNKDQENSREAKRQPFGVKDMNNNRAYEAEESSSGVFWFLKPCTFLVE</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="1">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC123430919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="SMR" id="A0A8I6XQC6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199880.1">
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    </dbReference>
    <dbReference type="GeneID" id="123430919"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.2HG0166040"/>
    </dbReference>
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        <property type="entry name" value="OS01G0306400 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="28"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="250" mass="27335" checksum="44546A275871AFFA" modified="2022-05-25" version="1">MPATPRPATCRRHCTPIQERRGSYMAHAGDMTAFYGAWVGREEEIVSDLTAALGARRRDALAPLVDAAMDHVATLYEHKASLADRDVVAALDQRWLNPLERTFLWAWGWRPALVFRFVDGSGIGPRQRRELEDLRAATAAAEKEVDREVAAVQESLAGPRVLEALRQRRQHPRNGVQADEAVAAVGQSLRVLLAAGDALRERTVRGVVGVLAPDAEQAGAFFAAMLRFHLGVHRAGRAWSSGHGGGRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XR07</accession>
    <name>A0A8I6XR07_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XR07"/>
    <dbReference type="SMR" id="A0A8I6XR07"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0550560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0550560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0550560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0550560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0550560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0550560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
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    <dbReference type="Pfam" id="PF07645">
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        <property type="entry name" value="ASX_HYDROXYL"/>
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        <location>
            <begin position="270"/>
            <end position="294"/>
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        <location>
            <begin position="225"/>
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    <feature type="domain" description="Protein kinase" evidence="11">
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    <feature type="binding site" evidence="9">
        <location>
            <position position="370"/>
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        <ligand>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="663" mass="73977" checksum="E02408FB8A4C510A" modified="2022-05-25" version="1">MFVQNVEVISISAPLGQVRVFNDISTYCYNTSSRAMVQNAWSLDFSRSPFRFSYVRNKLIVIGCNTLAYINNLGSTAKYTTACAAVCESPAALTNGSCVGAGCCQNDIPKGLRGYSFSFFDVYRKSNSTLFNPCGYAAMVEAETFSFISDFITTTRFNDTDDGRKPLVLDWVVGNATCEVARDMPSYACHSRNSMCVNSTNGQGYLCNCTDGYEGNPYLFDECRDVNECEKNSSICPKSATCHNTIGGYYCSCSAGRKFVMETKSCNPDITLIIGISIGVVVLVIIIFCIRVIFERRNLANIKKKYFQQHGGMLLFEKMKSDQGLSFTVFTEAELKQATNKFDESLILGHGGNGTVYKGIIKDITPVAIKRCALVDDRQKKEFGKEMLILSQINHKNIVKLLGCCLEVEVPMLVYEFIPKGTLFDLIHGKNHKQHLPFSSLLRIVNEAAEGLAFLHSYANPPILHGDVKTSNILLDENYMAKVSDFGASIIAPTDKAQFVTMVQGTCGYLDPEYMQTCCLTDKSDVYSFGVVLLEILTGEEPLKLEGPEVCRSLSSSFLLAMKGNNLDNMLDDQIKGHENMELIVGVADLARQCLEMCSENRPLMKEVSEELSRLRKLSRHPWIHHDIETDSFLGPIEIEQSSKYTEKDERMPINPNSFYLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU38</accession>
    <name>A0A8I6XU38_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Triticodae</taxon>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        </location>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="333" mass="37425" checksum="04E03E7052AF0974" modified="2022-05-25" version="1">MERMSSSYFKEVYTKDPTLVANEVLVCIIPKVTLEMNEALCKPFSEQEISDALFQIGPLKAPGCDGLPARFYQRNWSVLKPEITVAVQEFFNTGNMPEGVNDTAIVLIPKVPHPKELKDFRPISLCNMVYKIVSKCMVNILRPFLTELISENQSAFIPGRLISDNSIISFECIHHIQSMKENSPALCAYKLDLSKAYDRVDWDFLEMALMRWGFSQTWISRVMACVTSVKYSVKFNGKLLESFSPSRGLRQGDPLSPFLFLFFADALSALISKSMREDGLQGVKICRGAPEISHLLFADDSLLFFHATEQHAVLVKGLLNTFASATCQLINPS</sequence>
</entry>
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    <accession>A0A8I6XUQ3</accession>
    <name>A0A8I6XUQ3_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">Integral membrane protein TmpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123439962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972506.1">
        <property type="nucleotide sequence ID" value="XM_045116571.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0271430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0271430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0271430"/>
    </dbReference>
    <dbReference type="GeneID" id="123439962"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0224960.1">
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        <property type="gene ID" value="HORVU.MOREX.r2.3HG0224960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0271430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0271430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0271430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439962"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0271430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="60974" checksum="69987607A4F8EB64" modified="2022-05-25" version="1">MDEQGPAAKPMAMAIKFSSCRGVAFEIKPSPASPFALDTVASPSDSPPTESGGGRWVWFPQPFNRNYSTTSFLTARSRASTHFCDLDDISDGDTDDESVATDEEMALTAADVSRVQSRRSNAPTKPVRAAAGHSRLSVILLDQGLFTVYKRLFLLCVVLNAAGLATAATGHFPYAKRHAAVFAIGNILALTLCRSEAVLRAVFWLTVVLLGRTWVPVVVKTGVTAILQSLGGVHSGCGVSSLAWLVYALVQAVELRGVTPHEIVGVASAILGLVALSCLAAFPLVRHLHHNVFERTHRFAGWTALALLWAFVMLTAVYNPAIRSYDRLTVASLAKRQELWLTAAITFFTFLPWLTVRRVPVTVTAPSTRASILTFQGGVKAGLLGRISRSPLSEWHAFGIISNDEDTHAMLAGAVGDFTRGLVSDPPSHLWVRTVHFAGLPYLISMYQRVTMVATGSGICVFLSFLMQPSWAEVSLVWVAKEIDANYGDGITAMVTSNENLKGRVIVHDTMTMGRPDVSELSVVAAQRWNAEAVVVTSNPEGSRDVVRRCRKAGIPAFGPIWDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUS0</accession>
    <name>A0A8I6XUS0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUS0"/>
    <dbReference type="SMR" id="A0A8I6XUS0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0445130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0445130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0445130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF12">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0445130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="109" mass="11708" checksum="0D430AF65829C2F3" modified="2022-05-25" version="1">MEVDDAVPRGGGGGGESPHIRRLEESVVNRIAAGKVIHRTSSAVQDLVENTIDADSSTISVTVKDGSLKLIQVSDDGYVIRIISKKNRPRYASTSSRVQGDDCMEHLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVG5</accession>
    <name>A0A8I6XVG5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123445712</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 11S seed storage protein (globulins) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978673.1">
        <property type="nucleotide sequence ID" value="XM_045122738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVG5"/>
    <dbReference type="SMR" id="A0A8I6XVG5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329530"/>
    </dbReference>
    <dbReference type="GeneID" id="123445712"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0274420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0274420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0274420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0329530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445712"/>
    <dbReference type="OrthoDB" id="336321at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02243">
        <property type="entry name" value="cupin_11S_legumin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02242">
        <property type="entry name" value="cupin_11S_legumin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006044">
        <property type="entry name" value="11S_seedstore_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF40">
        <property type="entry name" value="CUPIN TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00439">
        <property type="entry name" value="11SGLOBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Cupin type-1" evidence="6">
        <location>
            <begin position="13"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="6">
        <location>
            <begin position="208"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="38881" checksum="141D034013647B81" modified="2022-05-25" version="1">MVQRTSNAEVMSMDLSPKKPAKAYGSDGGAYYDWSPADLPMLGAASIGAAKLHLSAGGLALPSYSDSAKIAYVLQGAGACGLVLPEAASEKVIPVKEGDTLALPFGAVTWWHNAEGASAELVVLFLGDTSKGHTPGRFTNFQLTGATGIFTGFSTEFVARAWDLDQDAAAKIVSTQPGSGIVKIAAGHRMPEPRPDDRQGVVVNCLDAPLDVDIPGGGRVVVLNTANLPPVKDVGLGSDLVRIDGRSMCSPGFSCDSAYQVTYIVRGGGRVQVVGIDGTRVLETRAEAGCLFIVPRFFVVSKIADDTGMEWFSIITTPNPIFSHLAGKTSVWKAISPAVLETAFNTTPEMEKLFRSKRLDSEIFFAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXX8</accession>
    <name>A0A8I6XXX8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123406055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXX8"/>
    <dbReference type="SMR" id="A0A8I6XXX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545580.1.CDS1"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0563390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="21841" checksum="33727F342AB8681E" modified="2022-05-25" version="1">MEFFTVILEKSSRLVLPHNFVKMLDGHRPQNMKLRQAGNGLRKLWDVEVVFDADGRMYLDRGWKQFVRAYDLGIGYFLVFRYDGNATCAVKVFDTTMCRRCYQDDDDAGNGSRSSEYCDRCYVYGSSDSGSKSSSDSGHSKSSSDDGVAMDVPQLGDRAMPIVVEEYIPQPGLGLRRSKRIKMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0644580.1">
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="16132" checksum="58A7F03621C7F524" modified="2022-05-25" version="1">MNFPDVRTLQQALDLAPPPRFNSAQDRAEHTAMQRRLLVAQEDEWVMAEWRRRHPEDVAYEQEYWERRREEDTRRRREERLDRRRRKALACAQADLVNAGGSSFFTEEDERWFDIWLSTSDDTDDDVGADDWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2U0</accession>
    <name>A0A8I6Y2U0_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">aldehyde oxygenase (deformylating)</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123402034</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a long-chain fatty aldehyde + 2 NADPH + O2 + H(+) = a long-chain alkane + formate + 2 NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:21440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83563"/>
            <dbReference type="EC" id="4.1.99.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.5" evidence="4"/>
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        <property type="nucleotide sequence ID" value="XM_045095892.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0595750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0595750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0595750"/>
    </dbReference>
    <dbReference type="GeneID" id="123402034"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0493720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0493720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0493720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0595750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0595750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0595750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402034"/>
    <dbReference type="OrthoDB" id="408954at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071771">
        <property type="term" value="F:aldehyde oxygenase (deformylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021940">
        <property type="entry name" value="CER1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12076">
        <property type="entry name" value="CER1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="13">
        <location>
            <begin position="136"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Very-long-chain aldehyde decarbonylase CER1-like C-terminal" evidence="14">
        <location>
            <begin position="453"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0595750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="619" mass="71439" checksum="2A05B3686D1B9D6C" modified="2022-05-25" version="1">MATRPGPLTEWPWQRMGNFKYLVMAPVVVHGAYRVMNKGWGDIDLAYALILPSLALRMIHNQIWISLSRYQTARSKHRIVDRGIEFDQVDRERGWDDQIVFNGLLFYVGYLAIPNVRGLPLWRTDGAVATALLHAGPVEFLYYWFHRALHHHFLYSRYHSHHHASIVTEPITSVIHPFGEHIVYFTLFAIPMLSTLYMGNGSALVFVMYIVYIDFMNNMGHCNFELVPKWMFQVFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYSTMDKASDELYENSLKGTEETPDLVHLTHMTNLQSAYHLRVGFASIASKPSDNSEWYMWTLWPLAWLSMVVAWIYGSSAFVVERIKLKKLKMQTWVVPRYNFQYGLTWDRESINDLIEKAILDADVRGVKVLSLGLLNQEKQLNGNGELFRQKYPKLGLRIVDGSGLATGVVLKSIPSDAKQVFLHTGTSKVARAVAMALCGKGIQVIMNRKKEYDMLKSQMPENRASYLKCSSNDITKIWLVERMEDKEQRMAPKGTVFIPISQFPLKKVRKDCTYLSTPAMKIPDTMQNIHSCENWLPRRVMSAWHIAGILHVLEGWSVHECGDDMMDSEKAWSAAIRHGFVPLTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4A1</accession>
    <name>A0A8I6Y4A1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123402980</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045096953.1"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.6HG0509430"/>
    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657:SF73">
        <property type="entry name" value="OS02G0752800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="171"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="330" mass="35545" checksum="722A7A323926E3CF" modified="2022-05-25" version="1">MAAAIDMYKYNPATHQIGSASDQELMKALEPFITGASSSSYPYPYQYQYYSSPSMTQDSYTATPSSSSSYASFAAPPLPTTAPFSQLPPLYSSSSQYAVNNGSMGLAQLGPAQIQQIQAQFFVQQQQQRGLAGGSFLGPRAQPMKQSGSPPPRASAAALALAGVAPAQSKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEDAALAYDKAAFRLRGDLARLNFPSLRRGGAHLAGPLHASVDAKLTAICQSLAAPSSKNSAEAEPEPESPKCSASTEGEDSVSAGSPPPVPEMEKLDFTEAPWDESETFHLRKYPSVEIDWDSILS</sequence>
</entry>
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    <accession>A0A8I6Y5N2</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5N2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0348690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0348690.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0348690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0348690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0348690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="18083" checksum="37DCDDE15A6CAC07" modified="2022-05-25" version="1">MLTSKMHAIWNPRHFIIVIYTKAKGTNPRQFEKRHPTLSTSSQNTSLLLGPPYADVEGVLVDEVEDTEVPDLDPVVAGEVAPPGLHACLVACQRQLHGPRSPARVLEYHQPLRRDAIHDAELLPDVLHEAQAKTNTISSCCSECCKELPSWRRKGGCRCSQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7D9</accession>
    <name>A0A8I6Y7D9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123451901</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044984389.1">
        <property type="nucleotide sequence ID" value="XM_045128454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7D9"/>
    <dbReference type="SMR" id="A0A8I6Y7D9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437110"/>
    </dbReference>
    <dbReference type="GeneID" id="123451901"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0361770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0361770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0361770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0437110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451901"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010928">
        <property type="term" value="P:regulation of auxin mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000142">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000058">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000081">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF948">
        <property type="entry name" value="OS12G0594300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="847"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="788"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="788"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="878"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1126" mass="123896" checksum="FFE854BC8386F12F" modified="2022-05-25" version="1">MNSQVGPNNHKNLNVVYQDLTTVLGLGRFDSGNLAAGLPLKCDMDPANLVRTRTIPSENVLKPAQHSSDNLQDTSSPCESPHAGKVKFMCSFGGKILPRPSDGVLRYVGGETRLISIPRNFSWKELVHKTLSIYNQPHTIKYQLPDEDLDALISLSCEEDLQNMMEEYYSLEKANGSPRLRIFLVSPNDCEESSLSARSLESEPEYHFVVAVNNLSQLKQSTSDNNLVSQLSQQLDNSLLPSRDSTVSQTDIESGSKALAGTALSESSSQFFLAPYSQQMVAESAATSSPGLNRQRTSLNQSRLRMSSDRLTMNQEHENKYEVSNGSNLKTMLPDHQDKTQSDVDTGIGIGSTMHNLQIQRQAKDLGTPRNESYVSSHTNYDKSTPMEMPFYSEKVYIHPETASWLSGMHEYTGQIHGMPHAFSDPLLNDRTEQPASNLSLTFDSYIPPSFSQKMCQANELERTISGTRPDLLRAKPPQIARMDEPNYLVSSHTDQRYGQGVTGGASFESAVYYQQDSLPTNMVQTGHDGGPVVQQRKIYHPENSAAPSVAPQCTYVDTRLTLHHTRGARLSSGELDALESSCLTPMHATDNSHSRFLHGFPNGSQTANSDRGSHVEKMKLGCVTDYGTAGCVHGNDKVAPGPHILLPIDPFEAFAPQRSVANGASAVYQNENFDQLLGHTSGLATSSSIGLSNPDLTVNMFGNGTFKDGLLQRDVPVFNQHNIICSDVGVIGSDRATINNEDMILKGRMHNNFQMEARVIVEDVTDDMPSCIPSSRPIPQVEMAAEEQHHAIMSSEKDDDAKSDGPELANEEHGDKAAADGSISDAEIAELEASMYGLQIIRNADLEELRELGSGTFGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQEKLTKDFWREAQILSKLHHPNVVAFYGVVPDGTGGTLATVAEFMVNGSLRNVLLRKDRSLDRRKKLIIAMDAAFGMEYLHSKSIVHFDLKCDNLLVNLRDPQRPICKVGDFGLSRIKRNTLVSGGVRGTLPWMAPELLNGSSSRVSEKVDVFSFGIALWEILTGEEPYANMHCGAIIGGIVNNSLRPPVPETCDPEWRILMEQCWSANPDIRPSFTEVTDRLRAMSAMLQSRVQAPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7I7</accession>
    <name>A0A8I6Y7I7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7I7"/>
    <dbReference type="SMR" id="A0A8I6Y7I7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0383180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="228" mass="24856" checksum="15E307130974CEEE" modified="2022-05-25" version="1">MPHLYDLYGMAHTASYKKAKAFSESNLDDPNNFTNISSHHKLVAYRDAGKATKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKARQSRSCPEIMRRPRPVDLAIEAALQKERLANQAALEKERLANQAALEKEKLASQAALQDALDERDQTTSRLIEEERSRNEAGQRALYELFVGLCEKGGQVPPPMPVFSSISTNNSGATSHDPSQGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y848</accession>
    <name>A0A8I6Y848_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123399496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949839.1">
        <property type="nucleotide sequence ID" value="XM_045093904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y848"/>
    <dbReference type="SMR" id="A0A8I6Y848"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0532630"/>
    </dbReference>
    <dbReference type="GeneID" id="123399496"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0442980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0442980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0442980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0532630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0532630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399496"/>
    <dbReference type="OrthoDB" id="5199543at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="44181" checksum="A9D1FE42F3FC0AA1" modified="2022-05-25" version="1">MDAAMEDAGAPPGSDWDRGSGGAEAVLGLAGAGASLSVCYHEAFGPHADLILLEAGDDLLPDLLQGRVTVRGRPEEEAVLCTPSATYAMKFVGTSNSMFLIPPGEAVAASLRPDHTNEDATVASDASASIIKVAPGSIELVRTAPRLDKLRSLLRERPYVLDEDLGDDSEDKKGLYTWEDLCVLVQSSDSELLEGLNSLSAVEIDGFWRTVDVNSVNTVLDMILHNSVLHDWLLNALPENGVLSVMESDGFTHKIVAHCLSRFGTKVEQEAGSCWRLDERLVCLQFARKALSAGKMKLNNFMDKWERSIPSGMRADLQMLEGEVLYERLGAETWVHAFSVADLPLTPAERFAALFGERPRWEWKDLQPFIRDLRVPGASSEGLLIKYARRTQPSADADPIFTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YA71</accession>
    <name>A0A8I6YA71_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibits both subtilisin and chymotrypsin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YA71"/>
    <dbReference type="SMR" id="A0A8I6YA71"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0457970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0457970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0457970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0551130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000864">
        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
        <property type="entry name" value="potato_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54654">
        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="73" mass="8089" checksum="8330E78DE193B9FC" modified="2022-05-25" version="1">MSTSGKSSWPELLGWPVRPAMLTVKNERPEVSIDQLPEGTTPTPPGFRSDRVVVFYHTQDELGRRVATIPVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCA6</accession>
    <name>A0A8I6YCA6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metal transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123411815</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960708.1">
        <property type="nucleotide sequence ID" value="XM_045104773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCA6"/>
    <dbReference type="SMR" id="A0A8I6YCA6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707710"/>
    </dbReference>
    <dbReference type="GeneID" id="123411815"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0707710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411815"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001923">
        <property type="entry name" value="PRK00701.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF41">
        <property type="entry name" value="METAL TRANSPORTER NRAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="57074" checksum="B10B77786D267C85" modified="2022-05-25" version="1">MKPVRETPAVLGKEEEVDIEAVAGLRGDDCGVNSQDPAWKRFLRHIGPGFMVSLAYLDPGNLETDLQAGANHRYELLWVILIGLIFALIIQSLSANLGVVTGRHLAELCKSEYPVWVKICLWLLAEVAVIAADIPEVIGTAFAFNLLFNIPVWIGVLIAGSSTLLLLGLQRYGVRKLEFLVALLVFVMAACFFVEMSIVKPPAKEVLKGLFIPRLNGSSATADAIALLGALVMPHNLFLHSALVLSRDTPASVRGMNDACRFFLFESGIALFVALLVNIAIISVSGTVCNAGNLSPENAAKCGDLTLDSSSFLLKNVLGRSSAIVYGVALLASGQSSSITGTYAGQYIMQGFLDIKMKKWVRNLMTRSIAIVPSLVVSIIGGSSGAGRLIIIASMILSFELPFALIPLLKFSSSRNKMGQSNNSIYIVGFSWTLGFIIIGINVYFLSSKLIGWILHNSLPTYANVLVGVTLFPLMLLYVASVIYLTFRKDTVKFMSRREQEDMDNTEKAKVANKDGSEDDRVVQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCB8</accession>
    <name>A0A8I6YCB8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCB8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0682830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0682830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0682830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0682830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0682830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0682830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0682830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28906" checksum="FCFA726796A49B24" modified="2022-05-25" version="1">MAGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLVVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRMDLYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARAFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFT4</accession>
    <name>A0A8I6YFT4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123409653</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958447.1">
        <property type="nucleotide sequence ID" value="XM_045102512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFT4"/>
    <dbReference type="SMR" id="A0A8I6YFT4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0634710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0634710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0634710"/>
    </dbReference>
    <dbReference type="GeneID" id="123409653"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0527250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0527250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0527250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0634710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0634710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0634710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409653"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000007">
        <property type="entry name" value="Signal peptide peptidase-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF90">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152961" description="PA domain-containing protein" evidence="12">
        <location>
            <begin position="30"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="100"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0634710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="534" mass="58287" checksum="B5E84F6A86059C3F" modified="2022-05-25" version="1">MAILRRRELLLPAVAAVLLLLLVAGRAAADDASGDDRGHDASPGCSNKFQLVKVKNWVNGSEGMTVVGLSARFGATLPRTVNEAQRASAVLTNPLDCCSNITSKLTNSIALATRGGCPFTAKAEFAQAAGAAGLIIINDDEELYKMVCGDNDTSINVTIPVIMIPHSAGKNLKYSLDHGARVEMQLYSPSRPVVDLSACFLLIMAVGTIVCASLWSEFVACEQIDEQYNQLTRQPGPNSGTNSTQDKEILEITAKGAGVFIIVASVFLLLLFYFMSSWIAWLLIVLFCIGGIEGMHVCLVTIISRIFKGWGNNTVQLPFYGEVLTLSVGILPFCMVFAILWAIYRHSSFAWIGQDILGICLMITVLQMARLPNIRVASALLSAAFVYDIFWVFISPLIFHESVMIAVASGDTSGETIPMLLRIPRFFDPWGGYDMIGFGDIIFPGLLVAFSYRFDRAGKKGILNGYFLWLTVGYAVGLFLTYLALFLMDGHGQPALLYLVPCTLGLIVVLGWIRGELPHLWNYGRRQEILVGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YH57</accession>
    <name>A0A8I6YH57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC123413102</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044961986.1">
        <property type="nucleotide sequence ID" value="XM_045106051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YH57"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751530"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051564">
        <property type="entry name" value="LRR_receptor-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055:SF63">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="101" mass="11351" checksum="A9D133AF18DBC642" modified="2022-05-25" version="1">MFTGRCPTDDMFRDGLTLQYFAEAAGLSGNVMEIADSNIWLHDEANNSVDAEHITRARECLAAIIQLGVLCLKQLPRERMSTSDATAEMHFIRDAYLSNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPW4</accession>
    <name>A0A8I6YPW4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123403908</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044953753.1">
        <property type="nucleotide sequence ID" value="XM_045097818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPW4"/>
    <dbReference type="SMR" id="A0A8I6YPW4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540820"/>
    </dbReference>
    <dbReference type="GeneID" id="123403908"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0449760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0449760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0449760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403908"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MADS-box transcription factor MADS-MC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
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        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="123"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="258" mass="28350" checksum="74710A03607CA0DC" modified="2022-05-25" version="1">MAPSSPEAAVPEGGEQQQIVAAAAAAAAKKGGGKRGRREMRRIEDATSRQVTFSKRRSGLLKKAFELGVLCDAEVALIVFSPRGRLYEYASAPDLQRTIDRYLNHTKGTSANQKTVEQPAAGVEMCRSEATALKHKIDAIEACQRKLSGEGLGSCSSHELHELELQLEKSLSCIRQKKQQKMLDKISELREKERKLLMENSVLREEYKALPLLELATAAAAETSPDGAGAEEDEEDERQRHYMEVETELVIGRPGSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXJ2</accession>
    <name>A0A8I6YXJ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Receptor kinase-like protein Xa21</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC123395141</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Receptor kinase that detects X.oryzae pv. oryzae protein Ax21 to promote innate immunity. Following X.oryzae pv. oryzae protein Ax21 detection, undergoes cleavage, releasing the processed protein kinase Xa21 chain.</text>
    </comment>
    <comment type="function">
        <text evidence="23">The processed protein kinase Xa21 chain released by protein cleavage after X.oryzae pv. oryzae protein Ax21 detection translocates into the nucleus where it can bind and regulate WRKY62, a transcription factor. Confers resistance to the bacterial pathogen X.oryzae pv. oryzae (Xoo).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044946009.1">
        <property type="nucleotide sequence ID" value="XM_045090074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXJ2"/>
    <dbReference type="SMR" id="A0A8I6YXJ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534730"/>
    </dbReference>
    <dbReference type="GeneID" id="123395141"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0444880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0444880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0444880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0534730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395141"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001158">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000432">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF83">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="632"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="662"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534730.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="936" mass="100905" checksum="F832AE48162CAF13" modified="2022-05-25" version="1">MLRNLSVLRLARNSLTGRIPLSLGSSSSISLVSVILANNSLTGPIPSALADSSSLQVLNLVRNNLDGEIPPALFNSTSLQRLALGWNNFSGSIPAVVPNFNSPLQALILSVNSLAGTIPSTLGNFSSLRILLLAANSFKGSIPVSIAKIPNLQELDISYNLLSGTLPAPIFNMSSITYLSLAVNSFVGELPFDMGYTLPSIQTLILQQNQVGGKIPPSLANATDFLSINLGANAFYGTIPSFGSLSNLEELILASNQLEAGDWSFLSSLANCTQLQVLSLGTNMMQGNLPSSVGRLATSLRALVLHANKMSGSVPAEIGNLTNLSFLRMEQNLFAGDLPEAIGNLVNLTSVDLSRNRLSGQIPRSIGKLRQLTKLLLQDNNISGPIPRELGDCQSLITLNLSCNALSENIPRELFFLDSLSAGLDLSHNQLSGQIPQEIGGLINIGPLNFSNNRLSGRIPTTLGACVRLESLHLEGNFLDGRIPQSFVNLGGISEIDLSRNNLSGEIPNFFQSFKSLKVLNLSFNDLNGQMPQGGIFENSSEVFVQGNSMLCSSSPMLQLPLCSASSRHRRTWRTVKITGISVAAIALVCLSCAVFILLKRRSKRSKHSDHPSYTEMKSFSYADLVKATNGFSRDNLVGSGAYGSVYKGVVESETNGMVVVKVFKLDQLGAPKSFVAECEAFRNTRHHNLVRVISACSTWDNKGNDFKALVIEYMANGTLESWIYSETRRPLSLGSRVTIAVDIAAALDYLHNSCVPPIVHCDLKPSNVLLDDDMDARLSDFGLAKFLQSHNCSSTTSTSLAGPRGSIGYIAPEYGIGNKISTAGDVYSYGIIILEMLTGKRPTDVLFKNGLSLQKFVGNAFPEKIREILDPNIIGDEVADHGNHAMVGMLSCIMQLVQVGLSCSKETPRDRPTMPDVYAEVSTIKREYSALRVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZG3</accession>
    <name>A0A8I6YZG3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="16131" checksum="CED1C4F670EDA5C3" modified="2022-05-25" version="1">MWHYTGPGDSTRTNVVGVTEERVTSWVLQITGPCENPKGARRVKPYSADHPPPNQAWNNWFSPVSNGNPEEEEEEGSQEGSVESAEYVSDSGESEEEKSEEEEEDEEQDSPPPQLEHRTKRRHEPAVPSAPPASSSAPPTAPVVPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z138</accession>
    <name>A0A8I6Z138_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z138"/>
    <dbReference type="SMR" id="A0A8I6Z138"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0649340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0649340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0649340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0539050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0539050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0539050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0649340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0649340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0649340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000537">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF115">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="103"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0649340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="448" mass="47872" checksum="5F386D4563677864" modified="2022-05-25" version="1">MSSRKAKVLAVAVAGTVLVAAEVALYLCLRLSRPFYLSTAAVLAATVVTLLLLLHCTAGRADRMAARRALDDGEELRVEYSYFRKVAGLPRKFSLHALAAATDDFRCVAGRGASGTVFRGVLDDGTPVAVKRIGDEGPVAGRADKEFRAEVAAIAGAQHVNLARLLGFCLGSPRFLVYEYMDHGSLDRWIFPSGADGDDGRPRGCLPWARRYQVAVDVAKALAYLHHDCRSKVLHLDVKPENILLDDGFRGILSDFGLSKLAGKDQSRVVTAVRGTAGYLAPEWLLGAGVTEKSDVYSYGMVLLELVAGRRCVRPEEDGGWSYLPKIAAEMARTGRVTEVLDGRLAGAGTGTGDAVRRAVHVALWCAQEKAGARPSMARVLEMLEGRVAGEVEAPPPSDTIMEDLLALGHARARGGGPFRLPSAGRAASSASVLSKYDSFAMSYLSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZFQ0</accession>
    <name>A0A8I6ZFQ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123407755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956891.1">
        <property type="nucleotide sequence ID" value="XM_045100956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZFQ0"/>
    <dbReference type="SMR" id="A0A8I6ZFQ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748380"/>
    </dbReference>
    <dbReference type="GeneID" id="123407755"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0620800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0620800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0620800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0748380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407755"/>
    <dbReference type="OrthoDB" id="512667at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF15">
        <property type="entry name" value="A_TM021B04.14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="3">
        <location>
            <begin position="165"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="254" mass="26856" checksum="7FE41E4C960F2ECF" modified="2022-05-25" version="1">MLILSSHVKCVSPCLLSHQSTPGRKRKGICSPPPPSMESVAIATTTTTRALPPPFPSAAHRLRRRAASFVPVAAASKRRDGGEGEDAGAGSRRPSSSSGGEPTGLAPYGISFSPFSKDAAMGLVMSAATGSGWTTGSGMEGPPTAGGAANRPEVSTLPWSLFTKSPRRRMRVAFTCNVCGQRTTRAINPHAYTDGTVFVQCCGCNIFHKLVDNLNLFHEMKCYVGPDFRYEGDAPFNYLDSGDDDGSGNIFPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1L3</accession>
    <name>A0A8I7B1L3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123449661</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982889.1">
        <property type="nucleotide sequence ID" value="XM_045126954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1L3"/>
    <dbReference type="SMR" id="A0A8I7B1L3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449661"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0070040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0070040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0070040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0085140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449661"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000041">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF31">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A 57 KDA REGULATORY SUBUNIT B' KAPPA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="500" mass="55663" checksum="9C6933DED1E5EAE3" modified="2022-05-25" version="1">MWKQFLSKLPRTKSSGGGGGGDSGQCSGGNGNPIQRTSSVPAGGGGRSASTIKRMSSAVFPSSVVAGIEPLVSFKDVPSSERHNLFVSKLGLCCAVFDFSDPAKSSAEKDIKRQTLLDLIDFVDSSSGRYPEAVIAACSRMLAVNLFRVFPPSYRSGGGSSGGGGGGEGDEDEPMFDPAWCHLQLVYELLLKFIGSNSLDAKIGKKYFDHSFIVRLLNLLDSEDPRERDSLKTILHRIYGKFMVHRPFIRKAVSNIFYHFVFETQRHNGIAELLEVFGSVISGFALPLKEEHKIFLWRVLIPLHKPKSVGVYLQQLTYCVTQFLEKDPKLAGSIIIGLLRYWPVTNSQKEVMFLSEIEEILEATSMVEFQKCMVPLFRRIAHCINSSHFQVAERALFMWNNDHIISLVAQNRQVIMPIIVPALEQNGQNHWNHAVLNLTANVMKMFSEMDEELFSVCLTKCKEDEENQVSMEEKRRLKWAKLESAAALQPVTGHTAVLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2D8</accession>
    <name>A0A8I7B2D8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Patatin</fullName>
            <ecNumber evidence="8">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123425611</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="function">
        <text evidence="6">Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Hydrolyzes phospholipids as well as galactolipids. May play a role in disease resistance.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the patatin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7B2D8"/>
    <dbReference type="SMR" id="A0A8I7B2D8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0122900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0122900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0122900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0122900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0122900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07214">
        <property type="entry name" value="Pat17_isozyme_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF26">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7 8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 7 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="25"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="7">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="7">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="7">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="412" mass="45138" checksum="F1C7E3E4B3AC0FE7" modified="2022-05-25" version="1">MASSSSAEEGERQNSADKDKLITVLSIDGGGVRGIIPATILGFLGEEFKKLDGPEARIADYFDVIAGTSTGGLLTVMLTAPDKNGRPLFDAKELAQFYMDESPKIFPQNCRNSFFGKIATALKMVRGPKYNGKYLHALLRRHLGETKLDRTLTNVVIPTFDIAYLQPSIFSSFQLKHSPAKNALLSDIAISTSAAPTFFPAHYFETKDDKGEPRAFNLIDGGLAANNPTLCAMSQVSQDIIVGDGEFFVQNPVDYGKFMIVSIGCGLNPKESYSAKDTAKWGILNWIVKDGTTPIVDMFNAASADMVDIHLSVLFGALRSSHRYLRIQYDQLSGSAGSIDDCSKENMDRLVQIGGELLRKNVSRVDLETGRNVEMPGQGTNAEQLAKFAKHLSDERRRRQKVLQINQQLAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B333</accession>
    <name>A0A8I7B333_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971399.1">
        <property type="nucleotide sequence ID" value="XM_045115464.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971400.1">
        <property type="nucleotide sequence ID" value="XM_045115465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B333"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0048910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0048910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0048910"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0048940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0048940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0048940"/>
    </dbReference>
    <dbReference type="GeneID" id="123433671"/>
    <dbReference type="GeneID" id="123433687"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0048910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0048910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0048910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0048940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0048940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0048940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123433671"/>
    <dbReference type="KEGG" id="hvg:123433687"/>
    <dbReference type="OrthoDB" id="597802at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057202">
        <property type="entry name" value="DUF7880"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074:SF135">
        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25306">
        <property type="entry name" value="DUF7880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1618" evidence="2">
        <location>
            <begin position="201"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7880" evidence="3">
        <location>
            <begin position="418"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0048910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="75673" checksum="7573AB349E63A90D" modified="2022-05-25" version="1">MALPTLPFNPPTPPPPGFPDSAILCKTARVSADRNATTAGCRTEDGQAVEVSFWLVHPPGISHFSVNCPGINREYEAWLVCAEDAFVLFSLQYWGPARFFVYTAGKHPSLRLLPNPSLPYFGGQQFGLLPRGDGEHYALAFLRCKWSLQDDVCRFDAHVFSSETRAWTTRRAYLSDPADKPLCGRNDLFNQIRVGATSLGWFDNHHGILLLDHLFATHPVISIIPLPVATVGLPMPIKPDDKYIASEYFYNVACCHDLIKFVHIKYDDPHALARGSGWKATMWNMNVSGKNWCKGYTVDVDEISVDKSFSAKLPELWDDETQQLQLKKLMFHGPILSTLNDDLLYMMAKVKHDDDTAWTIAIDMKHAALKALAKFSVGPNQQLLTTACFSCVFPKYLNIPPGAEMYDPLEMHFKRMSLVQFVMQVQRTREWFRELDLFLDCDLPTYKESKPLLTECCPVSSLCVHIRALLKYATCTDEASNNMQHLLRAFEGFDMLLTESFNEQASDETLRSKIIVALPILDNLLHSMLPTVVPEGRCQGGVGIFEQYEKSGYTKKGHQSCGSTADKVCYSKKRNRAKKRTHKQQQEISKPNYFGGNVALNRWWYLGGYMLMSIGMLALCWMILTTLEVWTEPASSNLTLRSFGTKLSVIYKYYTDTWSEDGRSCRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B352</accession>
    <name>A0A8I7B352_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426746</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966561.1">
        <property type="nucleotide sequence ID" value="XM_045110626.1"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0168770.1">
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
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        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="381"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="178"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0168770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="479" mass="51310" checksum="F95FD33D4EA5924A" modified="2022-05-25" version="1">MNLTLSLGVNGGGAAAKKRKVMDGVGDVGVLGAADGCDRATVMRLLQARDRMVKVELDHDVDGDVSGCGASPLMRLLLSSAVAGEVGDTHAAAVALQEVYRHASFRGGDPVQRVAAYFADALASRLLLSPQPAQATTRQEQFLAFTMFYQASPLYQFAHFTANQAIMEAFEAGGTRRLHVVDFDVSFGFQWPSLIQSLSDAAATSSSLSSHDDSGDSCDEPVSLRITGFGASADELRDTEARLARFADGCPNLRFEFEGVLNSGPEDDGRHDRIKTDPAATVVVNLVFPAAQNARAGSWDPRSALAHIWSLSPSLVFLIDKVDEGAGRCGGSASLLPRFAANLRYYAAVFESLHECLPADSAERIAIEKDHLGVEISDAMATLDRRRHDGDRAADQNQTSRGWKERLESAGFEDARLSSRTVSQAKLLLKMKSGGCGGGGFRVMEAGDGKAMALGWRDRALVTASAWRPCRRSVGKNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B494</accession>
    <name>A0A8I7B494_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B494"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0210770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210770"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF189">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-binding domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="198" mass="23201" checksum="B7B6389BD899931B" modified="2022-05-25" version="1">MPPRRRSASGYCGVRERPNDGFYAEIRSGDLRLSLGTYDTAHEAARAFDAAAWRLGRPRRQMNFQDVYTLQQASDVAPPSRVNSAQDHAEHTARQRRLLVAEEDERVMAEWRRCHPENVAYEQSYWARRREEDTRRRREERLDRRRRKALASAQADLVNAGGSSFFTEEDERWFDIWLSTSDDTNDDDDGADDWSDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4H0</accession>
    <name>A0A8I7B4H0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123442376</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974326.1">
        <property type="nucleotide sequence ID" value="XM_045118391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4H0"/>
    <dbReference type="SMR" id="A0A8I7B4H0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219670"/>
    </dbReference>
    <dbReference type="GeneID" id="123442376"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0182960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0182960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0182960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442376"/>
    <dbReference type="OMA" id="MCESDRN"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="U-box domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF65">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="4">
        <location>
            <begin position="32"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="102"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="359" mass="38274" checksum="9D6C44927C2EFD33" modified="2022-05-25" version="1">MESPEAVSPSSGRGAGERPSEAAALRALVERVRGGEVEAAREVRRLTRASARHRRKLAPAVEPLVAMLRSGKPAGAGEAALLALLNLAVRDERNKIKILDAGALDPLLGYLQSSDLNLQEYAAAAILTLSASSTNKPIISASGAIPLLVKVLKEGNPQAKNDAVLALYNLSTIADNLPTILSVQPVPPLLELLRGGKRSSKTADKCCALLESLLAFDQGRVALTSEEGGVLTIVEVLEEGSLQGREHAVGALLTMCESDRSKYRDSILNEGAIPGLLELTAHGTPKSRVKAHTLLDLLRDSPYSRSKLQPNTLENIVSNIASQIDGEDRGGKAKKMLAEMVKVSMEQSLRHLQRRASFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4J2</accession>
    <name>A0A8I7B4J2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123442446</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974429.1">
        <property type="nucleotide sequence ID" value="XM_045118494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4J2"/>
    <dbReference type="SMR" id="A0A8I7B4J2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222300"/>
    </dbReference>
    <dbReference type="GeneID" id="123442446"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0184770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0184770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0184770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0222300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442446"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001543">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="57"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="400" mass="43581" checksum="DAAD931D025C69C4" modified="2022-05-25" version="1">MPPPAAGSWAWACCGRGRRSKGGGQGDEDSAGASGREGGQWTLFIDLPVLEAATAGFSDRNLLGRGGFGPVYKGVMDGGQEVAVKRLSLESRQGVREFLNEVRLLLKVQHRNLVSLLGCCAAAGQKMLVYPYFPNGSLDHILFDREKRVQLDWPKRHQIILGLARGLLYLHEESPVKIIHRDIKASNVLLDEKLNPKISDFGMARLFLEDATHVNTFRISGTYGYMAPEYAMNGYLSTKTDVFSFGMLVLEIVSGRKNIDRHLGDEKVDLLNYTWKLSEEGRSLEIVDPGLSGWDAGEVGMCVQLGLLCCQAVVSERPDMYSVHLMLSSDSFTLPKPGKPAIHGRIGRWMSTTGSASSAASGGNTNSTFATDTTKASTLGNIAEDESRNSISISFTTEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8X4</accession>
    <name>A0A8I7B8X4_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8X4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0474660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0474660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0474660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0474660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0474660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0474660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0474660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="12985" checksum="D5B4B3A299227298" modified="2022-05-25" version="1">MVSNLNLALQVANPAFKDDISKWECIVSVVPTNSHCALSGYLVFNYMHSLCDGKLYFPIPEDCFELRKQFLVHILKYEENESQNNISYIEGSIIDRIYRWTFIASKDGPSRCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BA38</accession>
    <name>A0A8I7BA38_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123440060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972586.1">
        <property type="nucleotide sequence ID" value="XM_045116651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BA38"/>
    <dbReference type="SMR" id="A0A8I7BA38"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277270"/>
    </dbReference>
    <dbReference type="GeneID" id="123440060"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440060"/>
    <dbReference type="OrthoDB" id="1920326at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000054">
        <property type="entry name" value="Werner Syndrome-like exonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF83">
        <property type="entry name" value="OS01G0660800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="18"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="197" mass="21466" checksum="6B46844A97EE54C9" modified="2022-05-25" version="1">MATDTFVTDVAFGADVIVTTVTNSGAAVEVWLQEIRSVLGDLVVGIDVEWRPSYGPSQNPVALLQLCVGRRCLIFQLLHADFVPQALSGFLADPNLRFVGVGVQEDVERLSDDHALEVANAVDLRGLAAEGMQMPELRQAGLQAVARNVMGANLQKPQRVRMGPWDAYCLSYDQIKYACIDAFVSFEVGRKLLTGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAI1</accession>
    <name>A0A8I7BAI1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">S-adenosylmethionine transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123395096</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Plastid</location>
            <location evidence="12">Chloroplast membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044945963.1">
        <property type="nucleotide sequence ID" value="XM_045090028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAI1"/>
    <dbReference type="SMR" id="A0A8I7BAI1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533720"/>
    </dbReference>
    <dbReference type="GeneID" id="123395096"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0444030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0444030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0444030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0533720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395096"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000095">
        <property type="term" value="F:S-adenosyl-L-methionine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000038">
        <property type="entry name" value="S-adenosylmethionine carrier 1 chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="15"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="97"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="192"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="30977" checksum="6797A999679BA471" modified="2022-05-25" version="1">MGASGGGEDKPFNFLQILCEGVIAGGTAGVVVETALYPIDTIKTRLQAARAGSQIQWKGLYSGLGGNLVGVLPASALFVGIYEPTKRKLLDMFPENLSAVAHLTAGAVGGLGASLIRVPTEVVKQRMQTGQFRTAPDAVRLIVAKEGFRGLFAGYGSFLLRDLPFDAIQFCIYEQLRIGYKLMAKRELKDPENALIGAFAGAITGAITTPLDVLKTRLMIQGQTKQYSGIVSCAKTILREEGPGAFLKGIEPRVLWIGIGGSIFFGVLEKTKSVLAERSSRKAVLAEKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAY0</accession>
    <name>A0A8I7BAY0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LURP-one-related 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440762</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973246.1">
        <property type="nucleotide sequence ID" value="XM_045117311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAY0"/>
    <dbReference type="SMR" id="A0A8I7BAY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313130"/>
    </dbReference>
    <dbReference type="GeneID" id="123440762"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0261570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0261570.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0261570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0313130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440762"/>
    <dbReference type="OMA" id="GIFRRWE"/>
    <dbReference type="OrthoDB" id="652749at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF56">
        <property type="entry name" value="PROTEIN LURP-ONE-RELATED 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20914" checksum="332395664B181D77" modified="2022-05-25" version="1">MAKIQPLPAATSPSSSDDHRQGQQTYTVWMKSLVFNGNGCAVYGPDGAVAFRVDNYGCRGGREVFFMDRAGNALVRIRRKGFGAFRRWQVCRCDEEETTPWFSVRRAEKGGAAVAMHGGAGTCYRIDGCSGARKPEYRVRGVDGTVVAEVARKQTAAGVVLGQDVLTLTVAPEMDRLLVLGLVVVRGLISRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BC51</accession>
    <name>A0A8I7BC51_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC51"/>
    <dbReference type="SMR" id="A0A8I7BC51"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0497640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0497640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0497640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0600260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34480:SF14">
        <property type="entry name" value="OS01G0967800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34480">
        <property type="entry name" value="OS01G0967800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="49480" checksum="2484095DB23C3C1D" modified="2022-05-25" version="1">MPRNEIASTVSGGVMLPKDFLSPKHRRDQPLGSGTVHGGEPYLRPHTAQQATSSSESSARFPSAAEKTVAQVNSKKTKELEEADKEICRLNELGLGDTVSHEGYLVHLKQLPRKPDVDTSTELDAVQLKELNDCHASYRLKYYYHYQLSQHVPEDKLCYDKLKENYPAGHRPMGQRSFMCLEEDGELDWSFHLDYCKLAGLEDYQRLVPRNYGGFEYANWDEYHKDCHSYEIEQGYVKYCEALPNKLKWIEAYVLNKPPSLMWGKILTRGTYQAIKIATDFSEIYEGLAFSGFYEWLDSMGFDVCCYNEFDGLYYEIWQRYTVEKNFRDALYEVYELNMFPLRHERMRRALENDTFSSDLEEEFLACTACLEEFAEDKSLITEDKARELIAEALRKETQKLKFYEDYCRKKIDIAQAIGLISSIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCB9</accession>
    <name>A0A8I7BCB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123448750</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981678.1">
        <property type="nucleotide sequence ID" value="XM_045125743.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981681.1">
        <property type="nucleotide sequence ID" value="XM_045125746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCB9"/>
    <dbReference type="SMR" id="A0A8I7BCB9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379900.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379900.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379900"/>
    </dbReference>
    <dbReference type="GeneID" id="123448750"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379900.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379900.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448750"/>
    <dbReference type="OrthoDB" id="551907at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF2">
        <property type="entry name" value="MYB-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myb_DNA-binding"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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            <end position="104"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379900.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="354" mass="39446" checksum="21AEB34DBBBBDFF5" modified="2022-05-25" version="1">MYHHQQQLQSHGQLLASRQTFPSERHLLLQGGIIPGESGLILSTDAKPRLKWTPELHDRFVEAVNQLGGPDKATPKTIMRLMGVPGLTLYHLKSHLQKYRLSKNLHAQANVGNSRTAVGCTVATEKQSEGNGSPVGHHLNTQTNKSMHISEALQMQIEVQRRLHEQLEVQRHLQLRIEAQGKYLQSVLEKAHETLAKQNTGSGGLETAKMQLSELVSKVSTECFHNAFTSLGDNDGSVMLRRHTMQLADGSVDSCLTACEGSQRDQDILSISLSAQKGKEIGGMAFDLQMKERGHEDLFLNKLSNRPPNHQEGRERRDSLSMTYQATKLDLNMNDTNDGTQNGKKFDLNGFSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCG6</accession>
    <name>A0A8I7BCG6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCG6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0617780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31722:SF62">
        <property type="entry name" value="EMB|CAB62433.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31722">
        <property type="entry name" value="OS06G0675200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="17033" checksum="2EDCBFB56F43FB3C" modified="2022-05-25" version="1">MACVNMYNPDGGAVAFGGGQPGPRISFSSDFSMEPPPPVQNRAMGLRCQEEDQNFEFSVGSHPMMAADQLFSKGRILPFKVEGGRAPSTLRDELRGSADEERASTPKGSSRWREMLGLRKSLCVGGGVANNAAAAKKGDKVVAVDADAKIATDMAASKQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2CT87</accession>
    <name>F2CT87_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440345</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK354839">
        <property type="protein sequence ID" value="BAJ86058.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK358453">
        <property type="protein sequence ID" value="BAJ89666.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972855.1">
        <property type="nucleotide sequence ID" value="XM_045116920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CT87"/>
    <dbReference type="FunCoup" id="F2CT87">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_70169.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059870"/>
    </dbReference>
    <dbReference type="GeneID" id="123440345"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0047540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0047540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0047540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0059870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440345"/>
    <dbReference type="eggNOG" id="ENOG502S1RW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086191_0_0_1"/>
    <dbReference type="OMA" id="AQFLPRW"/>
    <dbReference type="OrthoDB" id="822750at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CT87">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044963">
        <property type="entry name" value="SDH4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36358">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36358:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ86058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="23716" checksum="2594A994D459B2C3" modified="2011-05-31" version="1">MASRFLARSKTLALALSRSDAAPAPLAGSRALSSLPRYPAAAASSPATGSPPGLGKVLGLEPTSHLSGTQFLPRWFSTVASNGSAMQNAQVSETCKSVAGTGHSNALKVTEGAFPKVAAFSPLEAAAKPRSSPLTSESSKVRRSEIATGVTFYMIPILLLSSRNSMSTSLMVAAVYHQIYMFHKEILLDYVHHDITRKWALIYFKLLLLVMAKDTIVYFGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXZ8</accession>
    <name>F2CXZ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123409920</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356502">
        <property type="protein sequence ID" value="BAJ87719.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958727.1">
        <property type="nucleotide sequence ID" value="XM_045102792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXZ8"/>
    <dbReference type="SMR" id="F2CXZ8"/>
    <dbReference type="STRING" id="112509.F2CXZ8"/>
    <dbReference type="PaxDb" id="4513-MLOC_61570.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0716110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0716110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0716110"/>
    </dbReference>
    <dbReference type="GeneID" id="123409920"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0594020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0594020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0594020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0716110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0716110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0716110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409920"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064842_0_0_1"/>
    <dbReference type="OMA" id="VIRMIHD"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044238">
        <property type="entry name" value="ASHR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47420">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47420:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="4"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ87719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0716110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="42207" checksum="F2EEDC665AC835B6" modified="2011-05-31" version="1">MSGDTLRVAELPGRGRALLAARDILEGEVLLSESPILLYPSSLASLSSYCSACFRSLPPPPHTPCPSCRAAAFCSPACAAASHPRLLCAALSSGLAAAPEAHQEPLLFLLSAYSLQEPSLSAILSLSSAPQGPSPSQQQEAASLHAAVASVAPPHMLPAGFSLDLTAALLSKDRTNSFSILEPYRPDVPLELRKARCCAVYPRASLLNHDCLPNACHFDYADRPGPGNTDMVVRALHGITEGNEVCISYFAANWRYADRQRRLLEDYGFRCECDRCQIESKWKLDDDNDGADGDDTMEDEEHGKEDAEDGGDEGMEQEEGSDDDDDDFPHAFFFVRYLCDRDDCYGMLAPLPPLPNGELSHVFECNACGQLKKEEDEDEPDAGDCSMDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZI2</accession>
    <name>F2CZI2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123396411</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357038">
        <property type="protein sequence ID" value="BAJ88253.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044947290.1">
        <property type="nucleotide sequence ID" value="XM_045091355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZI2"/>
    <dbReference type="SMR" id="F2CZI2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426180"/>
    </dbReference>
    <dbReference type="GeneID" id="123396411"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0354090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0354090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0354090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0426180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396411"/>
    <dbReference type="OMA" id="KQIMRAM"/>
    <dbReference type="OrthoDB" id="46529at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CZI2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000059">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF47">
        <property type="entry name" value="F19P19.27 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="72"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ88253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="162" mass="16942" checksum="06FD069727CEB35B" modified="2011-05-31" version="1">MASASASAAALEKARQLLEEAAAESLPAEQVDALPSGFYDAFVLCGIRVHAVEPGRLLCHFTVPARLLNSGNFLHGGATASLVDLVGTAVFYTAGAQTRGSPLEMNISYLDAAFSDEEIDIEAKVLRAGKAVGVATVELKKKSGKIIAQARYSKYLGASSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZZ8</accession>
    <name>F2CZZ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123412944</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDK5RAP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357205">
        <property type="protein sequence ID" value="BAJ88419.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961839.1">
        <property type="nucleotide sequence ID" value="XM_045105904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZZ8"/>
    <dbReference type="SMR" id="F2CZZ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0675400.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0675400.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0675400"/>
    </dbReference>
    <dbReference type="GeneID" id="123412944"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0560390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0560390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0560390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0675400.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0675400.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0675400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412944"/>
    <dbReference type="OrthoDB" id="340432at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008491">
        <property type="entry name" value="CDK5RAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14894">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14894:SF0">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05600">
        <property type="entry name" value="CDK5RAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ88419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0675400.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="563" mass="63087" checksum="0A455175AE37B629" modified="2011-05-31" version="1">MRDPSEIRSLPIDIAFARLQEWLVDRKRVPQDWRKRLAAIRARLAAAFSSLPRDLHPYLQTLELEEIGYLEAKKICSILLESNTDSRNIFGRLTGSAGEWESIVKAYEKDHVFLGEAAQIMVQNVNYDIPYQRKQMQKTQQQLAELDRREADIKRLAALSATRYAEACQELGLQGIDVRQELIESAKTLPSTFSKILEVLNSDTVSQAMEYYTTFVRDCHTEDKENYKSVVHNLKQLQANPPSLNVSVCKEVQNSLGDTSKAHGSCMDGGEPIDSNVPADDIDWDISVDNNGIDWDIGAVEQPVEESGNGFGSYEIIDANIELAGSENYGVGASAYPSADKEGLTCDTSENQICWDISTDNPEESATIENAPTESGQYECSTEERSQLLEKEYRNNILDDLLEVKSFLTLRLAEMRNADTSSLQHQVQAVSPFVLQQYAPDRLENMLVEVSSAISLLTNQKTLDLIMILNSKRFLDRLVSTLEEKKHHEVKLREGLGDLSVKRMELQNALSSSWPKQEAAITKTRELKKLCEATLSSMFDSRPVHIIGEINNLLSSSGSQLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F2D031</accession>
    <name>F2D031_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123447605</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357238">
        <property type="protein sequence ID" value="BAJ88452.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK364044">
        <property type="protein sequence ID" value="BAJ95247.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK365678">
        <property type="protein sequence ID" value="BAJ96881.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980147.1">
        <property type="nucleotide sequence ID" value="XM_045124212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D031"/>
    <dbReference type="SMR" id="F2D031"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338460"/>
    </dbReference>
    <dbReference type="GeneID" id="123447605"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0282340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0282340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0282340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0338460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447605"/>
    <dbReference type="HOGENOM" id="CLU_027634_5_1_1"/>
    <dbReference type="OrthoDB" id="415590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052700">
        <property type="entry name" value="Carb_kinase_PfkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43320:SF1">
        <property type="entry name" value="OS01G0105900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43320">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="82"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ88452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ95247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="39649" checksum="BA6A8A1572A3D481" modified="2011-05-31" version="1">MGAEAEQQQQQLPLPSPQAGKADPPAVVGLQVSALIDHVAHVDWSLLDRVPGDRGGSQQVSFEELDHILNEVNALILPSHDDPSPVRTMAGGSVANTVRGLSAGFGISTGIIGARGDDDQGILFVNNMSFSGVDLTRLRAKKGHTAQCACLVDASGNRTMRPCLSSAVKLQANEFTKEDFQGSKWLVVRYAQQNLAQIIEAIRVAKQEGLSVSLDLASFEMVRDYRSQLIALLETGNIDLCFANEDEAREIIGGGLTFDPEDALAFLSKHCKWAVVTLASKGCIAKHGKQVVQVPAIGESNAVDATGAGDLFASGFLYGLVKGLPLEECCKVGACSGGSVTRALGGEVRPENWQWMYKQMHAGGLLLPELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F2D1P2</accession>
    <name>F2D1P2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123449511</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AK357799">
        <property type="protein sequence ID" value="BAJ89013.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982691.1">
        <property type="nucleotide sequence ID" value="XM_045126756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D1P2"/>
    <dbReference type="SMR" id="F2D1P2"/>
    <dbReference type="STRING" id="112509.F2D1P2"/>
    <dbReference type="PaxDb" id="4513-MLOC_64948.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402390"/>
    </dbReference>
    <dbReference type="GeneID" id="123449511"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0334940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0334940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0334940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0402390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449511"/>
    <dbReference type="eggNOG" id="ENOG502QPKZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013238_2_1_1"/>
    <dbReference type="OMA" id="TNPWADE"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D1P2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000028">
        <property type="entry name" value="3-ketoacyl-CoA synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="12">
        <location>
            <begin position="79"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="13">
        <location>
            <begin position="383"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ89013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="509" mass="55600" checksum="860DD939F05B9632" modified="2011-05-31" version="1">MDRDLFRTVKQGTRNQARLLYHRLVCRLPHLLAVTLLLVVAPPLVSTLSIAALWSEARANAAVLVAACAGCVAAAYAYAVSRPRPVYLVDMAGYKPGPAHEATRAQAIRQFGLAGGFDDESMSFQKRMMERSGLGEATHFPASLMSIPVDMCLQTARDESEAVVFGVVDELLAKTGVRAQDIGVVIANSSLYSPTPSFVSLIVNRYRLRHDIVSHNLSGMGCSAGIIAIDLAKHLLQVHPDTYALVVSTENITLNAYLGNNRPMLVTNTLFRVGGAAVLLSNRRRERARAKYQLIHTVRTHRGASDRSYGCVTQEEDEAGHVGVSLSKELMSVAGEALRTNITTLGPLVLPLSEQLRFLATVVLKRVFRADVKPHIPDFTLALDHFCIHAGGRGVLDELERSLKLSAWHMEPSRMTLYRFGNTSSSSLWYELAYCEAKGRIKKGDRVWQIAFGSGFKCNSAVWKALRPVDAVAVGDSGSPWAQDVDVLPVHVPKVVPIDDDEASYKTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2D221</accession>
    <name>F2D221_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123411884</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AK357928">
        <property type="protein sequence ID" value="BAJ89142.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK358609">
        <property type="protein sequence ID" value="BAJ89822.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960780.1">
        <property type="nucleotide sequence ID" value="XM_045104845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D221"/>
    <dbReference type="STRING" id="112509.F2D221"/>
    <dbReference type="PaxDb" id="4513-MLOC_44342.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0711200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0711200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0711200"/>
    </dbReference>
    <dbReference type="GeneID" id="123411884"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0589870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0589870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0589870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0711200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0711200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0711200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411884"/>
    <dbReference type="eggNOG" id="KOG2288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040730_3_0_1"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D221">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000002">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025298">
        <property type="entry name" value="DUF4094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF5">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13334">
        <property type="entry name" value="DUF4094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4094" evidence="15">
        <location>
            <begin position="18"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ89142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0711200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="399" mass="44743" checksum="0F98D8E276DFA08C" modified="2011-05-31" version="1">MTRGLGAGDELLFRGTISRKWTSLLCLSSFCVGLIFTNRMWTVPESKEIIRRSALELDKMNLVSSSDCALKSINEPRDDIGQVQRIEDAIQTLDKTISNLEMELASAKATQDSILNGGVPSSEPTAKRKYFMVIGINTAFSSRKRRDSVRATWMPQGEKRRKMEEEKGIIVRFVIGHSATSGGILDRAIDAEDRKHGDFLRLDHVEGYLELAAKTKSYFAKAVSMWDAEYFVKVDDDVHVNIATLGGILARHRSKPRAYIGCMKSGPVLAQEGVKYHEPEYWKFGEWGNKYFRHATGQLYAISKDLASYISINQHVLHKYANEDVSIGAWFIGVDAEHVDDRRLCCGTHPDCERKAQSGNVCAASFDWSCSGICKSADRIKEVHRRCGESANAIWNATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2W4</accession>
    <name>F2D2W4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123452083</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YABBY family.</text>
    </comment>
    <dbReference type="EMBL" id="AK358221">
        <property type="protein sequence ID" value="BAJ89435.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984596.1">
        <property type="nucleotide sequence ID" value="XM_045128661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2W4"/>
    <dbReference type="SMR" id="F2D2W4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0507700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0507700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0507700"/>
    </dbReference>
    <dbReference type="GeneID" id="123452083"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0507700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0507700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0507700"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071156_0_1_1"/>
    <dbReference type="OMA" id="PGPQKVQ"/>
    <dbReference type="OrthoDB" id="667577at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D2W4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000012">
        <property type="entry name" value="axial regulator YABBY 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006780">
        <property type="entry name" value="YABBY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056775">
        <property type="entry name" value="YABBY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056776">
        <property type="entry name" value="YABBY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675:SF38">
        <property type="entry name" value="PROTEIN YABBY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675">
        <property type="entry name" value="PROTEIN YABBY 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04690">
        <property type="entry name" value="YABBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24868">
        <property type="entry name" value="YABBY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="YABBY N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="YABBY protein C-terminal" evidence="7">
        <location>
            <begin position="103"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ89435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0507700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="187" mass="21185" checksum="D6E648F6C1ABF048" modified="2011-05-31" version="1">MSAQIAPPEHVCYVHCNFCNTILAVSVPSNSMLNMVTVRCGHCTSLLSVNLRGLIQSPLPVQDHSQENFKAQNISFHGNYPDYGTSSKYRMPMMFSTKSDQEHMLHMRPAPEKRQRVPSAYNRFIKEEIRRIKANNPDISHREAFSTAAKNWAHFPNIHFGLGSNESSKKLDETIATPIPQKVQGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7E6</accession>
    <name>F2D7E6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123413330</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK359808">
        <property type="protein sequence ID" value="BAJ91017.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK367473">
        <property type="protein sequence ID" value="BAJ98676.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962206.1">
        <property type="nucleotide sequence ID" value="XM_045106271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D7E6"/>
    <dbReference type="SMR" id="F2D7E6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0729110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0729110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0729110"/>
    </dbReference>
    <dbReference type="GeneID" id="123413330"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0604840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0604840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0604840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0729110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0729110.1"/>
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    <dbReference type="OrthoDB" id="1928288at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33167:SF61">
        <property type="entry name" value="OS06G0694400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33167">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF863)-RELATED"/>
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    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ91017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ98676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0729110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="241" mass="27475" checksum="CBDF7B5470001DA6" modified="2011-05-31" version="1">MSRRCSGMATDLFRTHRILFALLEKGGINIIRELESRGSVQKLAKILLKASGGGLLNDILADIMDRYSKHDSKESLRRTIMEHDEVFRQQVHELHRLYRVQKALMAELRGEKHSFQLRTEDTREMVQGHRPNLKNSSCTSETSQSACLGNAQYSDTRQVPEQSFLQECKPVSCLNLFDEETSRSQERRPESSKSVEGESWSVSMEGDLDLKLSIAPSSNATKAPHWLFSDSRERNPSGQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7U6</accession>
    <name>F2D7U6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123427790</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK359958">
        <property type="protein sequence ID" value="BAJ91167.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045111909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D7U6"/>
    <dbReference type="SMR" id="F2D7U6"/>
    <dbReference type="FunCoup" id="F2D7U6">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2D7U6"/>
    <dbReference type="PaxDb" id="4513-MLOC_10399.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194820"/>
    </dbReference>
    <dbReference type="GeneID" id="123427790"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0161640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0161640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0161640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427790"/>
    <dbReference type="eggNOG" id="ENOG502SCSE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107796_0_0_1"/>
    <dbReference type="OMA" id="WEEPGIP"/>
    <dbReference type="OrthoDB" id="687570at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000096">
        <property type="entry name" value="BZIP transcription factor bZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF388">
        <property type="entry name" value="OS02G0833600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="54"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ91167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="131" mass="14476" checksum="2762E3D29B73AFF8" modified="2011-05-31" version="1">MSWEEPGSPQLSLSGFSSLPSISSTAVRPARLPSLSLSIGTGGEDQHLAVSSDDGQKSVRAMKNRESALRSRARKRAYTQELEKEVRRLVEDNLKLKRQCKQLQSEIAALNAQQPSNKQGSPHRRTSSTQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F2DAP5</accession>
    <name>F2DAP5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123447648</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK360959">
        <property type="protein sequence ID" value="BAJ92166.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980198.1">
        <property type="nucleotide sequence ID" value="XM_045124263.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980199.1">
        <property type="nucleotide sequence ID" value="XM_045124264.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980200.1">
        <property type="nucleotide sequence ID" value="XM_045124265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DAP5"/>
    <dbReference type="SMR" id="F2DAP5"/>
    <dbReference type="PaxDb" id="4513-MLOC_56117.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339300"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339300.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339300.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339300"/>
    </dbReference>
    <dbReference type="GeneID" id="123447648"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0283050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0283050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0283050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0339300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0339300.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339300.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447648"/>
    <dbReference type="eggNOG" id="ENOG502QRH8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1609391at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DAP5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18919">
        <property type="entry name" value="bHLH_AtBPE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000002">
        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024097">
        <property type="entry name" value="bHLH_ZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF312">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="144"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ92166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="316" mass="34798" checksum="6CD577157B235F3E" modified="2011-05-31" version="1">MYSGQQSDQAPAANSGKEFLEGNWGSVAMHQKMGYDSAAYGFQAYGMELEEGQGTFSQNIHMSDEHSGGVKKRKGTNDCIALLNPSASSKNVGDQQTEVSSQPESNSMERDNRKISPKTQSKEDSSDGDGTKENYVHLRAKRGQATNSHSLAERLRRKKISERMKLLQDLVPGCNKITGKAVMLDEIINYVQSLQRQVEFLSMKLATVNPELGFDIEQILSKQMMLSQDRHLGFYGADPGASALIAHFNQGMMHPDMICNASNPVGALHGTIHDISTMNQMPEMWETLQNIPHMNFNPAVAADSSTNNAGPMKIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F2DCX3</accession>
    <name>F2DCX3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Non-specific lipid-transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123442596</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK361740">
        <property type="protein sequence ID" value="BAJ92944.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974619.1">
        <property type="nucleotide sequence ID" value="XM_045118684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DCX3"/>
    <dbReference type="SMR" id="F2DCX3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227360"/>
    </dbReference>
    <dbReference type="GeneID" id="123442596"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0188660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0188660.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0188660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0227360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227360"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128423_4_0_1"/>
    <dbReference type="OrthoDB" id="770678at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DCX3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00597">
        <property type="entry name" value="PLANT_LTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090686" description="Non-specific lipid-transfer protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ92944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="11085" checksum="0CD95C6E0E591CEB" modified="2011-05-31" version="1">MARAAASQLVLVALVAAMLLVAADAAISCGQVSSALSPCISYARGNGAKPPAACCSGVKRLAGAAQSTADKQAACKCIKSAAGGLNAGKAAGIPSMCGVSVPYAISASVDCSKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2DGN2</accession>
    <name>F2DGN2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123448706</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK363049">
        <property type="protein sequence ID" value="BAJ94253.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="BAK06982.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045125677.1"/>
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    <dbReference type="SMR" id="F2DGN2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0378750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0378750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0378750"/>
    </dbReference>
    <dbReference type="GeneID" id="123448706"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0315000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0315000.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0378750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0378750.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123448706"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF55">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
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    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010962348" evidence="7">
        <location>
            <begin position="31"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="8">
        <location>
            <begin position="213"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="Pfam" id="PF06814"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ94253.1"/>
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        <source>
            <dbReference type="EMBL" id="BAK06982.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0378750.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="501" mass="56247" checksum="15C6984AF671E383" modified="2011-05-31" version="1">MRLPRRGARPSLAVLALALALALVARGADASIHEYYGGGFAPRANSFFFHGGSEGLYASESSSNSSNSFIRFDIVTFRRPQESAARHEEMQQKTGLVEAIIVEIQDRDKIGGSYLHSDAICCTPELDKEKSCKVGEVIIRPNPDNPDWPKRIQTFFDGKNEETTMGTQSVSINKTGMYYLYFMFCDPQLRGLKITGRTVWRNPHGYIPGKMAPMMTFFGFMSLAYLALGLLWFLQFVRCWKDILQLHYHITAVIALGMCEMAFWYFEYANFNSTGTRPIGITLWAVTFTAVKKTVSRLLLLVVSMGYGVVRPTLGGITYRVAALAVIYFTASEALELVENLGNINDFSGRTRLFLVLPVAILDATFIIWIFSSLSRTLEKLQLRRSMAKLELYRKFTNSLAMSVVISIAWIGYELYFNATDPLSELWRRAWVIPAFWNVLSYVLLAIICALWSPSRNPTGFAYSEETGDEADEEGLSLVGSAVKGTGDMVNMHVFTEDKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2DKG9</accession>
    <name>F2DKG9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123411181</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="AK364387">
        <property type="protein sequence ID" value="BAJ95590.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045104120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DKG9"/>
    <dbReference type="SMR" id="F2DKG9"/>
    <dbReference type="PaxDb" id="4513-MLOC_14320.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656000"/>
    </dbReference>
    <dbReference type="GeneID" id="123411181"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0544360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0544360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0656000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656000"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S29N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049393_4_1_1"/>
    <dbReference type="OMA" id="DARDWEM"/>
    <dbReference type="OrthoDB" id="778717at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DKG9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF38">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="89"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ95590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="168" mass="18533" checksum="1127C8B5E70C9817" modified="2011-05-31" version="1">MELELGLAPPNHAGQPAGLCGKRDRSAPAVAFGVTKATLPLFLLRDDDGDDSGEDGTAGARDWGTSNKRKRLVGWPPVKSAHRPRSHHNGHVKVKMEGVPIGRMVDLSRHASYHELHHTLRLMFPSSTVHHADPYAVTYEDGDGDWMLVGDVPWEEFSKSAKRLKILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2DPY0</accession>
    <name>F2DPY0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365948">
        <property type="protein sequence ID" value="BAJ97151.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DPY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0261850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0261850.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0261850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0313430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313430"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186364_0_1_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48175">
        <property type="entry name" value="OS04G0581700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48175:SF3">
        <property type="entry name" value="OS04G0581700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ97151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="67" mass="7348" checksum="E7E741914492020B" modified="2011-05-31" version="1">MILVAVMAELLEEYTAAVARALERLLCAAPLRILPRRVRFIVLRTLPFAAPSPPVPPPHAVPVLLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2DVG0</accession>
    <name>F2DVG0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123410559</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367878">
        <property type="protein sequence ID" value="BAJ99081.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK369497">
        <property type="protein sequence ID" value="BAK00698.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959436.1">
        <property type="nucleotide sequence ID" value="XM_045103501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DVG0"/>
    <dbReference type="SMR" id="F2DVG0"/>
    <dbReference type="PaxDb" id="4513-MLOC_39772.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708320"/>
    </dbReference>
    <dbReference type="GeneID" id="123410559"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410559"/>
    <dbReference type="eggNOG" id="KOG3358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="KPQHGTR"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DVG0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd23294">
        <property type="entry name" value="beta-trefoil_MIR_AtSDF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000068">
        <property type="entry name" value="Stromal cell-derived factor 2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090798" evidence="3">
        <location>
            <begin position="22"/>
            <end position="217"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="4">
        <location>
            <begin position="96"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="4">
        <location>
            <begin position="154"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ99081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXS0</accession>
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        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123395483</name>
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    <organism evidence="7">
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAJ99891.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946423.1">
        <property type="nucleotide sequence ID" value="XM_045090488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXS0"/>
    <dbReference type="SMR" id="F2DXS0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0448670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0448670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0448670"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0448670.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0448670.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0448670"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0371640"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0448670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0448670.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0448670.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0448670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395483"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ99891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0448670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="17383" checksum="F01C1FA8FB384DB5" modified="2011-05-31" version="1">MSVELVLWLFSFASVMALVGLTAYQLICLSDLEFDYINPYDSSSRINAVVIIEYTLQGVLCASFLLTLNWFPFLVMAPVTYYHVKLYMNRKHLIDVTEIFRQLHGEKKYRLIKLAFYFALFIISIYRLVMTAVLLFIDEDANLVETRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
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        <name evidence="4" type="primary">LOC123399717</name>
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    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK370924">
        <property type="protein sequence ID" value="BAK02122.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950041.1">
        <property type="nucleotide sequence ID" value="XM_045094106.1"/>
    </dbReference>
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    <dbReference type="STRING" id="112509.F2E450"/>
    <dbReference type="PaxDb" id="4513-MLOC_81383.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493450"/>
    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0408850.1">
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0408850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0493450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399717"/>
    <dbReference type="eggNOG" id="ENOG502QSFD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MKNSCQE"/>
    <dbReference type="OrthoDB" id="114080at2759"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027951">
        <property type="entry name" value="Nepro_N"/>
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    <dbReference type="PANTHER" id="PTHR34786">
        <property type="entry name" value="OS09G0504900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34786:SF1">
        <property type="entry name" value="OS09G0504900 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="NEPRO_N"/>
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    </dbReference>
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            <begin position="33"/>
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        </location>
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        <location>
            <begin position="1"/>
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14780"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK02122.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2EAQ8</accession>
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        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
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    </protein>
    <gene>
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    <organism evidence="3">
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAK04430.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="F2EAQ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195090.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195090.1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK04430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="96" mass="10487" checksum="0753A4EBA6B8EC0F" modified="2011-05-31" version="1">MAMANPMNKGRSLPKFGEWDVKNPATADGFTVIFQKARDDKKTTAGPGKSGVPPAFRNTDGGYGSVKSGNSYKYTRVATPKRAKKKSWFSCTSCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECC5</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="4">COBRA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123425406</name>
    </gene>
    <organism evidence="7">
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            <taxon>Poaceae</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
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            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Flower</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the COBRA family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373800">
        <property type="protein sequence ID" value="BAK04997.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965039.1">
        <property type="nucleotide sequence ID" value="XM_045109104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECC5"/>
    <dbReference type="PaxDb" id="4513-MLOC_75041.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127250"/>
    </dbReference>
    <dbReference type="GeneID" id="123425406"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0105020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0105020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0105020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0127250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425406"/>
    <dbReference type="eggNOG" id="ENOG502QTGW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038120_0_0_1"/>
    <dbReference type="OrthoDB" id="2012261at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2ECC5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052324">
        <property type="term" value="P:plant-type cell wall cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673:SF63">
        <property type="entry name" value="COBRA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038122">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090899" description="COBRA-like protein" evidence="5">
        <location>
            <begin position="34"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="6">
        <location>
            <begin position="228"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK04997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="50352" checksum="37B089563BAB1B15" modified="2011-05-31" version="1">MAAAAPRTSLLPSSIALLLLALALSALVPSSDAYDSLDPTGNITIKWDVRQWTDDGYVATVSLYNHQQYRHIQAPGWKLGWVWAEKEMIWKMTGAQTTEQGDCSRFKAEAHCCRTDPEVVDLLPGAPYNKQTSNCCKGGVLSSWGQDPANAIATFEVRVGQAGTSRSSIKAPTNFTLMAPGPGYTCGPAKKVERTKFIDGDARRTIQALETWNVTCTYSQFVAGRTPTCCVELSSFYNSTIVECNTCSCGCQDNSTSPVSCVKANSPYLASVVNGDSTNIPAPLVQCTSHMCPIRVHWHVKANYKEYWRVKITATNLNYRMNYSQWNLVAQHPNFANLTTIFSFKYKDLNPYGIINDTGMMWGIKYYNDLLMTADRNGNVQSELLFRKDPETFTFQKGWAFPRRVYFNGENCVMPPPDVYPWLPNASHQLSASLTIPFITVWTALAFLLVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECI7</accession>
    <name>F2ECI7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123405562</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK373862">
        <property type="protein sequence ID" value="BAK05059.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955145.1">
        <property type="nucleotide sequence ID" value="XM_045099210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECI7"/>
    <dbReference type="SMR" id="F2ECI7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0011080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0011080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0011080"/>
    </dbReference>
    <dbReference type="GeneID" id="123405562"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0008240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0008240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0008240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0011080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0011080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0011080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405562"/>
    <dbReference type="HOGENOM" id="CLU_030538_3_1_1"/>
    <dbReference type="OrthoDB" id="581180at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1320.10">
        <property type="entry name" value="Outer-capsid protein sigma 3, large lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF93">
        <property type="entry name" value="NEPROSIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043303113" evidence="1">
        <location>
            <begin position="27"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="2">
        <location>
            <begin position="58"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK05059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0011080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="310" mass="35104" checksum="D522EEA945B4CFEC" modified="2011-05-31" version="1">MGRGYVVISALLYFLCRTFHLTLVESIDAYVNLRMNEKNTTLLRPKPFHFPWRANITDEGSGIISHYAMWHTEPGQFYGLRADMSIWASPNQETSQESGASLQIYCQVGGNYNLIQAGFHISPSLYNNRDIRFFTYWTKDLKSKGCYNLKCPGFVSAGRANLVPGQAMTPPSIYGEQDHYVRLSLNKDPNSGDWVVYRHDLQKPSFLGHFPNKLCPGTPRIQALTGFVNYLKNAQGPPMGSGHFPDYDDKKSAYFKHIQNYNPNGHSSDLFGIPMVKLVDRPDCYRANDLFLEYKKGYMFNYGGPSGCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F2EDF9</accession>
    <name>F2EDF9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446242</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Flower</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK374184">
        <property type="protein sequence ID" value="BAK05381.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978852.1">
        <property type="nucleotide sequence ID" value="XM_045122917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EDF9"/>
    <dbReference type="SMR" id="F2EDF9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0354080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0354080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0354080"/>
    </dbReference>
    <dbReference type="GeneID" id="123446242"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0295340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0295340.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0295340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0354080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0354080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0354080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446242"/>
    <dbReference type="eggNOG" id="ENOG502R5W1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="685977at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EDF9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAK05381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0354080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="15916" checksum="A517794945E28F41" modified="2011-05-31" version="1">MATDSSYPVQFFHRGGGGDGQWCSLGAAYAAVRVLRPQGHSLVMYSGPDKQPHQSQRIVFAYPILAGDAFERLDGSTLSWAEQGSGDEFALCFLDEASCAAVCGAISPVTTALAALDGIADRLEGLRVAAAEVGASAAGGDIACRLAQLSLAPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2EEP8</accession>
    <name>F2EEP8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123399299</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Flower</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374624">
        <property type="protein sequence ID" value="BAK05820.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949649.1">
        <property type="nucleotide sequence ID" value="XM_045093714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EEP8"/>
    <dbReference type="SMR" id="F2EEP8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0490190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0490190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0490190"/>
    </dbReference>
    <dbReference type="GeneID" id="123399299"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0490190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0490190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0490190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399299"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF181">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="43"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAK05820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0490190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="528" mass="58329" checksum="5647A67434D27975" modified="2011-05-31" version="1">METAAHYAAAPHQGAMPGFGGAVDEQQAAGVGILLQISMLVLSFVLGHLLRRRKFYYLPEASASLLIGMIVGGFANISNTQKSTRRWFNFREDFFLLFLLPPIIFQSGFNLAPKPFFSNFGAIITFAILGTFIASIVTGLLVYLAGLIFIIYRLPLVECMMFGALVSATDPVTVLSIFQELGTDVNLYALVFGESVLNDAVAISLYRTMASMRTSSSGQNLLIVILRFLENFVGSMSSGIGVGLISALLFKYAELGVENLHNLESCLFVLFPYFSYMLAEGLGLSGIVSILFTGIVMKRYTFYNLSEDSQRFTARFFHLLSSLAEAFIFIYMGFDIAMERQSWSHIGFIFFSIVFILVARAANVFSCAYILNLARPPHSQIPREYQLALWYSGLRGAMAFALALQSVHDLPEGHGETIFTATTSIVVLTVLLIGGSTGTMLEALQVVGDSNRYRHPYEENFEGSSEEGTPSKNKFQLKLRELRRSTSSFALLDRNYLTPIFTSQHEDDDDDDDNDDDSSGEVLGIAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFA5</accession>
    <name>F2EFA5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123401473</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Seed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK374831">
        <property type="protein sequence ID" value="BAK06027.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951232.1">
        <property type="nucleotide sequence ID" value="XM_045095297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFA5"/>
    <dbReference type="SMR" id="F2EFA5"/>
    <dbReference type="PaxDb" id="4513-MLOC_73230.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609910"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609910.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609910.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609910"/>
    </dbReference>
    <dbReference type="GeneID" id="123401473"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0505730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0505730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0505730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0609910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0609910.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609910.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609910"/>
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    <dbReference type="KEGG" id="hvg:123401473"/>
    <dbReference type="eggNOG" id="KOG0046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EFA5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21293">
        <property type="entry name" value="CH_AtFIM_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21299">
        <property type="entry name" value="CH_AtFIM_like_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21302">
        <property type="entry name" value="CH_AtFIM_like_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fimbrin-1 isoform A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fimbrin-2 isoform A"/>
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        <property type="entry name" value="Fimbrin-2 like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fimbrin-2 like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF79">
        <property type="entry name" value="FIMBRIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="133"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="278"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="404"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="528"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="644"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="667"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="732"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK06027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="746" mass="81738" checksum="DAB308752D0A9D5D" modified="2011-05-31" version="1">MSGFVGVVVSDPSLQGQFTQVELRSLKAKFVSLKRDSGHVTTKNLPGLMKKLRGLHEVVAEEEIAAFLSESYPDSDQEIEFESFLREYLNLQARVSAKEGNAAAAGGGGAGGRKNSSSFLKSTITTLLHNLNQAEKSSYVAHINTYLGEDPFLKKYLPIDPSGNQLFDLIRDGVLLCKLINVAVPGTIDERAINKKRVLNPWERNENHTLCLNSAKAIGCTVVNIGTQDLVEGRPHLVLGLISQIIKIQLLADLNLKKTPQLVELFDDSKDIDEVLSLSPEKMLLRWMNHHLKKAGYKKTVNNFSSDVKDGEAYAYLLKALAPETSPETTLETKDPDERAKMVLEQAEKLDCKRYLTPKDITEGSANLNLAFVAQIFQHRNGLTSDIKQVTLTQTASRDDVLVSREERAFRMWINSLGVESYVNNVFEDVRNGWVLLEVLDKVSPGSVNWKLASKPPIKLPFRKLENCNQVVKIGKELKFSLVNLAGNDIVQGNKKLIVALLWQLMRFNILQLLNRLRSHSKGSQGKQITDADILNWANSKVKASGRTSRMESFKDKSLSNGVFFLELLSAVQPRVVNWKVVTKGEADEEKKLNATYIISVARKLGCSVFLLPEDIIEVNQKMILTLTASIMYWSLLRQPQPEISEASEPSSMASDATSDIGSEDGASTAAPSESEEANSLSDSLSTLTTDDATSNAPAAENGNDATPNALPAGNGNDATPNAPPAENGNDATPNAPPAENGNNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>M0UFW3</accession>
    <name>M0UFW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123401617</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951387.1">
        <property type="nucleotide sequence ID" value="XM_045095452.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951388.1">
        <property type="nucleotide sequence ID" value="XM_045095453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UFW3"/>
    <dbReference type="SMR" id="M0UFW3"/>
    <dbReference type="STRING" id="112509.M0UFW3"/>
    <dbReference type="PaxDb" id="4513-MLOC_10585.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607410"/>
    </dbReference>
    <dbReference type="GeneID" id="123401617"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0607410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401617"/>
    <dbReference type="eggNOG" id="KOG1803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0UFW3"/>
    <dbReference type="OMA" id="KRVVKWI"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UFW3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.270:FF:000003">
        <property type="entry name" value="DNA-binding protein SMUBP-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048761">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF3">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21138">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="496"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="515"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="601"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="976" mass="106829" checksum="C8008600AB7813B5" modified="2013-04-03" version="1">MSLDLFSSRFLFSSGAAKKLPSRRRVTFVAAAPSSASTPSPARSQDASSSSRQRSVRVAPLGPSKGAPRPAGGNGRTRRRRQRRTVEGGQEQGGCVPSVEEASIRVGTLYENGDPLGRKELGRCVVEWLRQGMQSMASKFASAELQGEMADLTTAALTLNWGSAEGQLGFVIQAQPYLSPVPMPKGLEALCFKACTHYPTLFDHFQRELRGVLLSYQNQGLIYDWRSTQTWKLLKEMANSTHHRAAVRRTTPRTKAVHSSIGISLKKVRLMQDRIEDFVRHMSDLLRIERDAELEFTQEELNATTMLDNNSKPSKPVEYLVTHGQAQQEQCDTICNLSVISSSTGLGGLHLVLFRIEGEHKLPPTTLSPGDMVCVRTCNSRGEGATSCMQGFLYNLGEDGCSITVALESRHGDPTFSRLFGKSVRIDRIQGLADALTYERNLEALMLLQRNGLLKSNASIDVVATLFGDGKDVMKMEQNCLIDWGESSLPDPRLSERYAFDDSQLRALSLGLNKKRPVLVIQGPPGTGKTVLLTELIVRAVRQGENVLVTAPSNAAVDNMVEKLSSTGLNIVRVGNPARISPSVTSKSLAEIVNGRLSQFRKELERKRTDLRKDLRDCIEDDSLAAGIRQLLKQLGRDLEMKEKETIMEVLSDAQVVLSTNTGAADPLIRKIGCFDLVIIDEAGQGIEPSCWIPILQGKRCILAGDHCQLAPAILSRKALEGGLGKSMMERASLLHDGLLTTRLTVQHRMHDSIASWASKEMYQGLLQSSHSVASHLLADSPVVKDTWITRCPLLLLDTRMPYGILNADCVEHLDPAGTGSFYNDGEADIVTQHVLNLVHCGVSPSEIAIQSPYIAQVQLLREKIEEYPGLSGVEVSTVDSFQGREADAVVLSMVRSNPLGAVGFMGDSRLMNVAITRARRHVTVVCDTSTICHSTFLARLLRHIRRYGQVKHVAPGSLDGVSGLGFSQPTLPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0XGW0</accession>
    <name>M0XGW0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tyrosine-specific transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123449338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044982466.1">
        <property type="nucleotide sequence ID" value="XM_045126531.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982467.1">
        <property type="nucleotide sequence ID" value="XM_045126532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XGW0"/>
    <dbReference type="PaxDb" id="4513-MLOC_59938.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0397300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0397300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0397300"/>
    </dbReference>
    <dbReference type="GeneID" id="123449338"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0330530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0330530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0330530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0397300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0397300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0397300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449338"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XGW0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018227">
        <property type="entry name" value="Amino_acid_transport_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47715">
        <property type="entry name" value="TRYPTOPHAN/TYROSINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47715:SF1">
        <property type="entry name" value="TRYPTOPHAN_TYROSINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03222">
        <property type="entry name" value="Trp_Tyr_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0397300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="50797" checksum="F754705130B24D31" modified="2013-04-03" version="1">MFLCCRRHLTFSPLAAAVPSPSGHRPLKPRTPRAPILASKNPPPRPWPLPRRQWRWRCSSADAVQPPPLVEASVSGGGEKKSFWAAVSLIIGTAVGPGMLALPSATILSGPVPSTVAILLSWAYVVSSIVLVAELSFAAMESGGVDEVSFTGLASSTLGATFGGVVAVVYAALSFSLMVACVAGIGSLVSQLFPAVNPVLANALFPCFAAVLIAFFPFKAVDGVNRVLCGLMLVSITSLVVTGVSVGRSSMLNSLGFACWSPSAILPAIPVTVLTLGFHVITPFICNIVGDSVYDARRAILIGGAVPLVMVLSWNAVILGLAGAAGSARFEDPIKLLLSVNPAALAPVRGFAFAALATSLIGYAVSFPKQLQDTLQLIVKRFSLKQGSVELSDAGGGHGRNEVALTLAVLIIPIFIVSFFSTAFAKALDFAGVYANCFLFGILPPAMAWIHRSQKKRSPGSCEDILPGGNAALLVLFIIAVILAFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0XHX4</accession>
    <name>M0XHX4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123402757</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044952644.1">
        <property type="nucleotide sequence ID" value="XM_045096709.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952646.1">
        <property type="nucleotide sequence ID" value="XM_045096711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XHX4"/>
    <dbReference type="SMR" id="M0XHX4"/>
    <dbReference type="PaxDb" id="4513-MLOC_60173.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540760"/>
    </dbReference>
    <dbReference type="GeneID" id="123402757"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0449710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0449710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0449710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402757"/>
    <dbReference type="eggNOG" id="KOG4208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21467at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XHX4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="214" mass="24737" checksum="76E61073DBAF0916" modified="2013-04-03" version="1">MGLRDKKRNQRRVLSRRSAGPKTGDGKDFLPLEGKEQRIREKKQAEEPENTATVLYIGHVPHGFYEDQMQGFFQQFGAVKRVRVARNRKTGKSKHYGFIEFENPEVAKIVADEMNNYLLFEHTLQIALVPPEKVHAKLWKGVRKGFIPVDRVAIERNRLSKDKTVEEHKKMVEGIVKRDEKRRKRIKAAGIDYECPALIGSVQPSAKKIKFDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y4G0</accession>
    <name>M0Y4G0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="3 9">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123411548</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_044960481.1">
        <property type="nucleotide sequence ID" value="XM_045104546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y4G0"/>
    <dbReference type="SMR" id="M0Y4G0"/>
    <dbReference type="PaxDb" id="4513-MLOC_65618.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015130"/>
    </dbReference>
    <dbReference type="GeneID" id="123411548"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0011580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0011580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0011580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0015130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411548"/>
    <dbReference type="eggNOG" id="KOG0169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y4G0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000060">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF154">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="584" mass="65975" checksum="FF6ABFFA5BE3C8CE" modified="2013-04-03" version="1">MTTYRVCCFLRRFRPASNEPSEAIGDVFQAYAGADGALGEEALRRFLREVQGEAGDDDVEAAAREVMAFAAEHRLLKKGGGGLTVEGFHRWLCSDANAALHPRRGVHDDMGLPLSHYFIYTGHNSYLTGNQLSSGCSEVPIAKALRDGVRVIELDLWPNAAKDDVEVLHGRTWTSPVDLMKCLEAIKEHAFISSPYPVILTLEDHLTPHLQAKVAKMIKETFGDLLYLSESEAMPVFPSPEDLRGKIIISTKPPKEYLETKSSKEEAQNGSTEEESVWGDEIPDNKAQVATACQVSEKDSERYVEEDEEMEKKVQLGVNAEYKTLISISLTRRKHDMDQDLKVDPEKVSRMSLGEAAYEKATITHGSEIIKFTQRNLLRIFPKTTRITSSNYNPLMGWSYGAQMVAANMQGHGRKLWLTKGMFRANGGCGYVKKPDFLMNTDKMFDPRSKLPVKTRLKVTVYMGDGWRFDFRKTHFDKCSPPDFYARVGIAGVVADTMMKETKVIMDNWIPTWDHEFEFPLAVPELALLRVEVHESDNHQKDDFAGQTCLPVWELRSGIRSVRLYARDGEVLRSVKLLMRFEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>M0YCD9</accession>
    <name>M0YCD9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444741</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044977498.1">
        <property type="nucleotide sequence ID" value="XM_045121563.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977499.1">
        <property type="nucleotide sequence ID" value="XM_045121564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YCD9"/>
    <dbReference type="SMR" id="M0YCD9"/>
    <dbReference type="STRING" id="112509.M0YCD9"/>
    <dbReference type="PaxDb" id="4513-MLOC_67858.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298090"/>
    </dbReference>
    <dbReference type="GeneID" id="123444741"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0248400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0248400.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0248400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0298090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444741"/>
    <dbReference type="eggNOG" id="ENOG502QTFC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YCD9"/>
    <dbReference type="OMA" id="ILVFREY"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YCD9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000590">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000228">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000215">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053211">
        <property type="entry name" value="DNA_repair-toleration"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060">
        <property type="entry name" value="DNA DAMAGE-REPAIR/TOLERATION PROTEIN DRT100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060:SF21">
        <property type="entry name" value="L DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="7">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="683" mass="75171" checksum="8B1D2E8908A41EB9" modified="2013-04-03" version="1">MPTSPLQIYFWCILLLANVGISTSLRLERDALLDIKSCVEDPQNYLSNWDESHSPCQFHGVTCNKISGEVTGVSLSNASLSGTISPSFSLLHQLRTLDLSANSISGIIPAALTNCTNLQVLNLSMNSLTGQLHDLSSLLKLQVLDLSTNSFSGAFPVWIGMLSGLTELGLGENSFDEAGVPESIGLLKNLTWLFLGQCNLRGEIPASVFHLESLGTLDFSRNQMTGVFPKAISNLRNLWKIELYQNNLTGEIPPELAHLTLLSEFDVSQNQLTGVLPKEIASLKKLKVFHIYRNNFYGELPAGLGDWEFLESFSTYENQFSGKFPANLGRFSPLNAIDISENYFTGEFPKFLCQSNKLQFLLALSNNFSGEFPTSYSSCKTLERFRVSQNQFSGSIPHGMWGLPNAVIIDVANNGFTGGISSDISISATLTQLLLQNNNFSSELPVELGNLSQLQKLVASNNRFSGQIPAQIGNLKQLSYLHLEQNALEGSIPPEIGLCNSLVDLNLAENSMSGQIPDTVGSLLMLNSLNLSYNMFSGEIPDALQSLRLSYVDFSHNNLSGPIPPQLLMIAEDDAFSENSDLCVPDTPERWRQSATSLRSCQWIDNHRSFSRRRLFAVLIMVISLVVLLSGLACLRYENDRLEDINRKQDTESGDDSDSKWIAESFHARGVNTSAALPVPMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0Z6Z9</accession>
    <name>M0Z6Z9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor GTE4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123406381</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044955824.1">
        <property type="nucleotide sequence ID" value="XM_045099889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Z6Z9"/>
    <dbReference type="SMR" id="M0Z6Z9"/>
    <dbReference type="PaxDb" id="4513-MLOC_78113.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0584320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0584320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0584320"/>
    </dbReference>
    <dbReference type="GeneID" id="123406381"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0484350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0484350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0484350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0584320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0584320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0584320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406381"/>
    <dbReference type="eggNOG" id="KOG1474">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="RWDAEIK"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Z6Z9">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05506">
        <property type="entry name" value="Bromo_plant1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037377">
        <property type="entry name" value="GTE_bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="583"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="439"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="590"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0584320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="70027" checksum="5AD4D7A94E1E36C4" modified="2013-04-03" version="1">MSRWAPEIKVYSRRNPRKNPKPPPEDPTPNPNPPPEPSPDPNPHPEPRPIPNSDPLEQTLASFRRSIRRSESEDAVPPLSDPAAPTSPSPLSPTSSLHGAPAAASGDFSSGLARDGAVVPNGHADIDVRAAADDKARKRRVRSELRRQLASELDQVRVLSKRLKAAGEALAVEASQPVPRPPPLLTAGYVHPQFSGSDAVTPVPAPVTASVPPVRSFLQRRPLIVPEVHIESLDKEKRTPKANQLYQNSEFLLAKDRIPPSDSHGRKKTKHHKKKHRSSTGAGYNAEQRLYSHAFKKSSSLLCRLMKHKFGWVFNKPVDPVALGLHDYFAIIKHPMDLGTIKGQLTRGHYRDPKEFANDVRLTFHNAMTYNPKGQDVHFMAEQLLGIFEAQWPEIEAEVDYLASCPPLPKKFPPPPIDLRLLERSDSLKPHMALDSKSRPISHTPISVRTPSLKKPKAKDLDKRDMTIDEKRKLSNNLQNLPPEKLDIVVQIIKNKNLSVRQHEDEIEVEIDSMDAETLWELDRFVANFKKNLSKQKRKAERAMLARQDVELRALHAAQQTSQQPNVGEKSPKLNLMVSEQLATSVPDQNNNNGPSASRSSSSSSSSSDSDSSSSDSDSDSSSTDGSNAANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-03-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q8VWW3</accession>
    <name>Q8VWW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GST6</name>
        <name evidence="11" type="synonym">LOC123421365</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Physiol. Plantarum" volume="116" first="336" last="344">
            <title>Cloning and characterization of a glutathione S-transferase induced by a herbicide safener in barley (Hordeum vulgare).</title>
            <authorList>
                <person name="Scalla R."/>
                <person name="Roulet A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoots treated with herbicide safener</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="AF430069">
        <property type="protein sequence ID" value="AAL73394.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK360057">
        <property type="protein sequence ID" value="BAJ91266.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK364486">
        <property type="protein sequence ID" value="BAJ95689.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK370190">
        <property type="protein sequence ID" value="BAK01391.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8VWW3"/>
    <dbReference type="SMR" id="Q8VWW3"/>
    <dbReference type="PaxDb" id="4513-MLOC_18718.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0013750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0013750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0013750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0017720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017720"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_5_1_1"/>
    <dbReference type="OMA" id="ITVFHAP"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q8VWW3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009635">
        <property type="term" value="P:response to herbicide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
        <property type="entry name" value="GST_N_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF49">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01154">
        <property type="entry name" value="Main.5:_Phi-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="91"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAL73394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ91266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ95689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="219" mass="24905" checksum="6834DA900A82FDFE" modified="2002-03-01" version="1">MAPVKVFGPAMSTNVARVLVCLEEVGAEYEVVDIDFKAMEHKSPEHLVRNPFGQIPAFQDGDLLLFESRAIAKYVLRKYKTDEVDLLREGNLKEAAMVDVWTEVDAHTYNPALSPIVYECLINPLMRGLPTNQTVVDESLEKLKKVLEVYEARLSQHKYLAGDFVSFADLNHFPYTFYFMATPHAALFDSYPHVKAWWESLMARPAIKKLAAQMVPKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287NFI1</accession>
    <name>A0A287NFI1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123446169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978782.1">
        <property type="nucleotide sequence ID" value="XM_045122847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287NFI1"/>
    <dbReference type="SMR" id="A0A287NFI1"/>
    <dbReference type="FunCoup" id="A0A287NFI1">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287NFI1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0347910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0347910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0347910"/>
    </dbReference>
    <dbReference type="GeneID" id="123446169"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0290280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0290280.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0290280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0347910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0347910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0347910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446169"/>
    <dbReference type="InParanoid" id="A0A287NFI1"/>
    <dbReference type="OMA" id="VEYNCYI"/>
    <dbReference type="OrthoDB" id="1864232at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019056" description="Dirigent protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0347910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="19754" checksum="9164940DDA7E0A2C" modified="2017-11-22" version="1">MAKGVVVLLVLFTALSAVSGAHRPGNRALAHGRRPGADPAAQTHLHFYFHDSSRGASPSAMRVAGPANEPLQTLFGAVYVMDDPLTEKPELGSAAVGRAQGVYIGADQTAMGFLQIMNLVLTSAPYNGSSLTVLGRNFPLAKVREMPIVGGTGAFRFARGYAQARTYSMNIKTGDANVEYDVYVMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287RJM6</accession>
    <name>A0A287RJM6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287RJM6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0163520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0163520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0163520"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423260"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423460"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0483380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0483380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0483380"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0483680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0483680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0483680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0163520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0163520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0163520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0423260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0423460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0483380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0483380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0483380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0483680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0483680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0483680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="65" mass="7347" checksum="F2FF80DD46A648CA" modified="2017-11-22" version="1">MTKDETLLVFTLVVSSSSVSVFLFGILLFMVLISATRDFRERTKSKLVKIMIWAGTVLGSFDRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WBQ4</accession>
    <name>A0A8I6WBQ4_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6WBQ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0025010.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0025010.1">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0025010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15752" checksum="3418648F5860FB3E" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDVEVCEPEIPTHIKILVEKLNLVEKENKYLKEKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAVGSIVTLVVILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <lineage>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WE90"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0061970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0061970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0061970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="12266" checksum="77476FAC4549C4E6" modified="2022-05-25" version="1">MVTLSSLLSTMSPVTLNLPPPSSSTPSYRLNNVADDMEAFLSDIMDDGALSDGLMDDEYYLFAGEGYDADVTNIDQPVDSSNTGPIDLDPFDYVYSNIPDSTHILKHAANCDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIR2</accession>
    <name>A0A8I6WIR2_HORVV</name>
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        <recommendedName>
            <fullName evidence="10">Equilibrative nucleoside transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429868</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969779.1">
        <property type="nucleotide sequence ID" value="XM_045113844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIR2"/>
    <dbReference type="SMR" id="A0A8I6WIR2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135870"/>
    </dbReference>
    <dbReference type="GeneID" id="123429868"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0111940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0111940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0111940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0135870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429868"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF59">
        <property type="entry name" value="OS07G0557200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="45724" checksum="37C20EF74C85BAD2" modified="2022-05-25" version="1">MAYGEDGHRGVHTIPEGKSLAVFICWLLGNGCLFGYNSMLTMEDYFVHLFPSYHPVRVITLTYQPFVLVIAAVFAYHEAKVNTRVRNLVGYSLFFLSSLALIILDLATSGRGGIAAFIGVCTVVSVFGVAEGHVEGAMTGDLSLMCPEFIQSFSAGMAASGAITSALRLVTKAAFEKSRDGLRRGAMLFFAASCVFEVLCLVLYAHVFPKLPIVKFYRAKAASEGSLTVAADLAAAGLQRHPKLSAEDDPAIPERLSNKQLLMLNMDYALDMFLIYLLTLSIFPGFLAEDLESHSLGSWYALVLIATYNSSDLVGRYVPLMESVKVTSRRRLLAMVLARYLLLPAFYCAARYGGESWTIVLVSALGLSNGYLTVCVLTEAPKPYKGPEKNALGNLLVFCLLAGICLGAVLDWLWLIGKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJB7</accession>
    <name>A0A8I6WJB7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJB7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0205730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0171150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0171150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0171150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0205730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0205730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48124">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48124:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18347" checksum="4A5514129BAD13BC" modified="2022-05-25" version="1">MSLTSSSSSAVHHRALALPLIRCAGCKEKVRMFVSTTEKHDGWVFYKCQNHGVSCEFWRWELEYVQYLVDNHYLVGDAAVDAIGAAEERREQLLNAQELHYMNGSTSMSKKFVKRESGNVMTRQQADALMKLGREMVMLMKILLAVVLMLGVGALVLYMVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNP5</accession>
    <name>A0A8I6WNP5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429446</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044969416.1">
        <property type="nucleotide sequence ID" value="XM_045113481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNP5"/>
    <dbReference type="SMR" id="A0A8I6WNP5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0111890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0111890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0111890"/>
    </dbReference>
    <dbReference type="GeneID" id="123429446"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0091840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0091840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0091840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0111890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0111890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0111890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429446"/>
    <dbReference type="OrthoDB" id="2021064at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="113"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0111890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="352" mass="36762" checksum="92992E02724A45BC" modified="2022-05-25" version="1">MASSGGGRSPVTAVLDDLVDVRDRVAVLQTVLHESSPGPTTVEAGELVDGMMAKLSSAFSVVLGTGDGGVASSLSGAGRGPSGRRKRPGAASSGPHRRSSPRGRMKSPLSRTVTATTLTDGKSWRKYGQKQIHDSTNPRSYYRCTHLPDQGCKAKRHVQQSESNPTEYTMDYYGQHTCRDPSTFPSLIVQSAAAAPPPDCANLISFAPINGANSGFTASTSTSAFAHHLMKEAADHHPMLFSRFSNYYSSSPPAQEGVSSGSPSPACHGKFMQYGAGGQFINVTGLRTSPLTVGSAPAEYWPVVGVAGVDMDAGAGMDSFPSPPSSPGFLSGSLEGSFGNNVCDDDLFSFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WP85</accession>
    <name>A0A8I6WP85_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3''-deamino-3''-oxonicotianamine reductase</fullName>
            <ecNumber evidence="9">1.1.1.285</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123426847</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of a 3''-keto intermediate during the biosynthesis of 2'-deoxymugineic acid (DMA) from L-Met. Involved in the formation of phytosiderophores (MAs) belonging to the mugineic acid family and required to acquire iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-deoxymugineate + NAD(+) = 3''-deamino-3''-oxonicotianamine + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58487"/>
            <dbReference type="ChEBI" id="CHEBI:58685"/>
            <dbReference type="EC" id="1.1.1.285"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2'-deoxymugineate + NADP(+) = 3''-deamino-3''-oxonicotianamine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16137"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58487"/>
            <dbReference type="ChEBI" id="CHEBI:58685"/>
            <dbReference type="EC" id="1.1.1.285"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.285" evidence="9"/>
    <dbReference type="RefSeq" id="XP_044966678.1">
        <property type="nucleotide sequence ID" value="XM_045110743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WP85"/>
    <dbReference type="SMR" id="A0A8I6WP85"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171730"/>
    </dbReference>
    <dbReference type="GeneID" id="123426847"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0141850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0141850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0141850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0171730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426847"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033707">
        <property type="term" value="F:3''-deamino-3''-oxonicotianamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990641">
        <property type="term" value="P:response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AKR"/>
    </dbReference>
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        <property type="entry name" value="AKR4A/B"/>
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        <property type="entry name" value="Aldo/ket_reductase_CS"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0521">NADP</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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            <begin position="67"/>
            <end position="337"/>
        </location>
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        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00066850"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="40466" checksum="BD7E6048A6D00AA7" modified="2022-05-25" version="1">MAVIQLASHFSRSSKFVGSDSTIVPTRILPRPRGITRSAMASPSGSSRSPKIPEFPAGSGGRPVPAVGIGTASFPFVAEDVKNAVLAALELGYRHLDTASVYPSEHLVGEAVAEAARRGVIASREEVFVTTKVWCTQCHPDLVLPSLKDSLQNLQMEYVDLYLVHWPMSVKPSKPHFPMKREDIVQMDLKGVWQAMEECHSLGLAKMIGVSNFTTKKLQGLLAIAKIPPAVNQVELNPAWQQKKLIELCKEKGIHVTAYSPLGGQSRTSKINAVLQSEILKEIAEARGKSVAQISLRWIFEQGASMVAKSMKKERLQENLEIFDWELTDEDRFKITQIPQYKKVTVSAILCPEGVPGVDLSEVDVVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Triticodae</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="123" mass="14482" checksum="174BF7340474ABBA" modified="2022-05-25" version="1">MSLPCSDQSIRPKKMKSASLPRGVEAVRCWCDDLCKVKEVEVFSDWLGMKFFMCANYEFDPPESISAYISPPYPPPLCMYYRWIDTEMLDWAVTEIRERGRRAWVSWDLEERREKAEAEEKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQF7</accession>
    <name>A0A8I6WQF7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123443153</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045119436.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975372.1">
        <property type="nucleotide sequence ID" value="XM_045119437.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975373.1">
        <property type="nucleotide sequence ID" value="XM_045119438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQF7"/>
    <dbReference type="SMR" id="A0A8I6WQF7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245900"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245900.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245900.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245900"/>
    </dbReference>
    <dbReference type="GeneID" id="123443153"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0245900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0245900.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245900.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443153"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17980">
        <property type="entry name" value="DEXHc_DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000767">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001326">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="62"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="266"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="701" mass="78377" checksum="AB0334B5F521CC1E" modified="2022-05-25" version="1">MSKFWRPGSEKPSASLVDDEEGGVLFLPTNTSASSSSSGFGYASLERLRQRLPVYKYRKAILYLVERHGTTIIVGETGSGKSTQIPQYLKEAGWAEGGRLIGCTQPRRLAVQTVASRVAEEVGVKLGEEVGYTIRFEDETNPGMTMIKFLTDGVLIREMMEDPLLSKYSVIMIDEAHERSISTDILLGLLKKIQRLRPELRLIISSATIEARSMSTFFSNRRKNSLLKPADGLPNPEPAILSVEGRGYTVETHYLEEPVSDYLQAAVNTVLIIHEKEPPGDILVFLTGQDDIDAALKLLNDEIQHLGKHYFDLVILPLYSGLPRGDQDLIFAPTSKGKRKVVISTNIAETSLTLEGVVYVVDSGFSKQKCYNPISDLESLVVAPISKASARQRAGRAGRVRPGKCFRLYTEEYYLNEMQSDGIPEMQRSNLVSCIIQLKALGIDNILGFDWPASPSPEAMIRALEILFSLGILDEDAKLTVPIGFQVAEMPLDPMISKMILSANDFGCSDEILTIAAFLSVQSVWVSMRGVKKEFDEAKLRFAAAEGDHVTFLNIYKGFHQSGKSSQWCYKNFLNHQALKKVIEIRAQLVRVMKRFGIQLKSCDRDMQAVRKAIIAGSFANSCHLEEYGQNGMYKTIRTSQEVYIHPSSVLFRVNPKWVVYQSLVSTDKHYMRNVIAIEPSWLTEAAPHFYQFRTPNPAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQK9</accession>
    <name>A0A8I6WQK9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123405729</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQK9"/>
    <dbReference type="SMR" id="A0A8I6WQK9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0012370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0012370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0012370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0009350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0009350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0009350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0012370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0012370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0012370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="62"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0012370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="477" mass="52699" checksum="CFB114492565B0A6" modified="2022-05-25" version="1">MEVDRSIELREIEQGGTVPPSSSSPGLDVMEEYVETVKERESEAKLKDVNLDDWLPITSSRTAKWYYSAFHNVTAMVGAGVLGLPFAMSHLGWGPGVAVIASSFGITLYTLWQMVEMHEMVPGKRFDRYHELGQHAFGKKLGLWIIVPQQLVVDVGTDIVYMVTGGQSLKKFHDLVCNGRCRDIRLTFFIMIFGSVHFLLSQMPNFNSISGVSAAAAVMSICYSMVAFFASVVHKHPVGAAVVDYGLKATSTAGHVFGAFNALGAVAFAFAGHNVVLEIQATIPSTPEQPSKKPMWRGVMVAYAIVALCYFSVAFGGYYAFGNSVDPNILITLDKPRWLIAMANLMVVVHVIGSYQVFAMPVFDMMETVLVKKLKFTPGLPLRLTARSAYVALTMLIGMTFPFFDGLLGFFGGFAFAPTTYFLPCIIWLILRKPARYSTTWFLNWIFIVIGVLLMLLSPIGGLRQIILDAKTFKFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQT3</accession>
    <name>A0A8I6WQT3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQT3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0013470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013470.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26351" checksum="69E5D83F82B07B00" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARPALLQNSEFCYVLASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDASALRMELYATRARLWTVLTQLAPVVQTGYGEMEMLNPVRTHVPAHVDWPAIGGVTPLRGPLLRPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLCEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR67</accession>
    <name>A0A8I6WR67_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR67"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0021930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0021930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0021930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        </location>
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        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="278" mass="30489" checksum="6FBA025FE6C4C8A1" modified="2022-05-25" version="1">MAAHRQRCVKLMVSYGGRIERAQGRPPRYVDGEHLLLNVVSSVSPRGFRDLLAARAGFSDFSVKYCYSGEGLDSLCDVDTDEDLRDMLDLVLYRDLQVRLFNDLNLRRFRVYLFRDASAPSPTSKALGRPAPMQRSATSPALLPAKATEGLAPPAPSPVRRIATSPNTFWETPTAGAAPSKPPLAPALPRRIASTSLLTADSTEDSTDDTASWTTTTSTSTARATQHTPPHAAAFRPVEQSNPMCHEATFWPAEQSNPMCHAAPVFLVPVMPQYFWCQ</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6WRR8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0145460.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0145460.1">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0145460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="74" mass="8131" checksum="EB0595A2710E03A1" modified="2022-05-25" version="1">MDLLQGRYITSVSRDMAPPAKAAFVESYASARLAFVLVSGAFIFMMMLLMHGKISQSAESLKYVLAMICLHSVN</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
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            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="13869" checksum="1A88C46110800478" modified="2022-05-25" version="1">MVTLSSLLSTMSPVTLNLPPPSSSTPSYRLNNVAADMEAFLCGIMDDNALSDGLMDDGLMDDEYYLFAGEGYDADDTDIDQPMDSSKTGHIDLDPFDYVYSNIPDSTHILKHAANCDHCKAKKFQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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                <person name="Mascher M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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            <end position="36"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="15214" checksum="71DC7433A26B135F" modified="2022-05-25" version="1">MFGDITSDGTDHWNPASGESHADGKGKRLGRVVDGNNKKQKTSLVIQEQITRVGDITERTQSSFESYMKKEESSSVTSVMDLVVECGAIVGSDEYFIASELFVKREQRDMFLHMTESVAHFDWLRRKYNTNYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="16">MDR-like ABC transporter</fullName>
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    <reference evidence="15" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970239.1">
        <property type="nucleotide sequence ID" value="XM_045114304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WVM0"/>
    <dbReference type="SMR" id="A0A8I6WVM0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176070"/>
    </dbReference>
    <dbReference type="GeneID" id="123430432"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0145580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0145580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0145580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0176070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430432"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000251">
        <property type="entry name" value="ABC transporter B family member 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45136">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45136:SF2">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="715"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="796"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="28"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="354"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="676"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1007"/>
            <end position="1244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1262" mass="135994" checksum="F912E160763DF321" modified="2022-05-25" version="1">MGDAAAGKASFMEMVRYADTHDLCLMMLGVLGSFGDGIMQPLLMLVIGDIINSYGAAGSAGSAFSSSDVDKFALRLLYVAIAVGACSFLEGVCWTRTAERQASRMRRLYLEAVLRQEVHFFDAAPSSQSTTFGIITTISDDADTIQDFLSEKLPMVLANVTLFFGAMSVCFVFAWRLALAGLPLTFLFFVPSIVLGKRMVTAAGESRAAYEAAGGIADQAVSSIRTVASYNGERQTLERFRSALARSTALGIKQGLIKGAVIGSMGAIYAVWSFMSWLGSVLVIHQHAQGGHVFAAAICIVLAGISIMAALPNLRYFVDASAAAARMRGMIEKLPPLKEAGKKGATKDVVRGQIVFKDVHFTYPSRPDTRVLNGVNLTMSEGATIGLVGGSGSGKSTVIALLQRFYRPDSGEILLDGDDIGSLNAEWLRSKIGLVSQEPVLFATSIRENILFGNETASPEQIIDAAKMANAHVFITKLPNGYDTHVGQFGTQMSGGQKQRIAIARALIRDPKILLLDEATSALDSESERTVQDALDRASVGRTTVIVAHRLSTLRKADTIAVLDEGRVVEFGTHDELVGMDGGEGGVYAKMVHLQSSSVARADGPGVVEEEEKFHSVEIALPAGELRSSPVSSFRSAEPTVEISQLAVPVAHAANPEKSLHLRLLKMNRPEWKQALLGCAGAVIFGAVMPLYSYIMGSLPAVYFLSDHDLIRAKTRVYSLIFLAIAIVCITANIVEHYNFGVMGERLTERVRDQMLAKILSFEVGWFDEDENSSAAVCARLATQATKVRSLVGDRMCLLVQAGATASLGFALALYVSWRLALVMMAMQPMVIASFYFKKVLMTAGSRKAKKAQVQGSQLASEAVVNHRTITAFSSQRRMLQLYEAAQVGPRKDTITQSWFSGFCLCLCQFNATSSTALALWYGGKLMAGGQINTTQLFQVFFILMSMGRVIADAGSLTSDLAQGGDAVRSIIDTLDREPAIKAGTMDGASESDDDKEKMQKEIKGAIEFRDVHFSYPTRPEVTVLAGLSLEIGAGKTVALVGPSGSGKSTVIGLIERFYDVRSGSILIDGKDIRGYSLTHLRSHVALVSQEPTLFSGTIRDNIMYGDEQATEEEVRSAAALANAHEFISGMESGYETHIGERGAQLSGGQRQRIALARAVLKNARILLLDEATSALDTVSERLVQDAVDRMLQGTRTCVVVAHRLSTVHNSDMIAVVKDGRVAERGTHRDLVALGRAGMYYNLIKLQHGASPCHSPVRAVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WWF1</accession>
    <name>A0A8I6WWF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123427355</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967316.1">
        <property type="nucleotide sequence ID" value="XM_045111381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WWF1"/>
    <dbReference type="SMR" id="A0A8I6WWF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0184420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0184420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0184420"/>
    </dbReference>
    <dbReference type="GeneID" id="123427355"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0152650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0152650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0152650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0184420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0184420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0184420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427355"/>
    <dbReference type="OrthoDB" id="1915375at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047627">
        <property type="term" value="F:adenylylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01278">
        <property type="entry name" value="aprataxin_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF5">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="5">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="4">
        <location>
            <begin position="120"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="3">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0184420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="19024" checksum="B6553265FBA9EAA4" modified="2022-05-25" version="1">MKKSFCFRGSAAKRAGRTPMADWCVFCDIARRAPASTTALLYSDDKVVAFQDINPSAFRHYLVIPIEHIPTVKNLQRTNEDHQLVSHMVKVGKDLLSQDAPESEEHRFGFHQPPFNSIDHLHLHCLALPFIPSWRQVKYTPLGPLGGFIDVEKLLEKIKPETEVYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WY05</accession>
    <name>A0A8I6WY05_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123425059</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964659.1">
        <property type="nucleotide sequence ID" value="XM_045108724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WY05"/>
    <dbReference type="SMR" id="A0A8I6WY05"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0119710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0119710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0119710"/>
    </dbReference>
    <dbReference type="GeneID" id="123425059"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0098510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0098510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0098510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0119710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0119710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0119710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425059"/>
    <dbReference type="OMA" id="ETTVWRL"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF17">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="236"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="573"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="646"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0119710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="822" mass="90755" checksum="D45668525AB3DFC6" modified="2022-05-25" version="1">MASSSSSRGGGGDDDNEPYLVGFVVAKIVGLKYYTGTLSGGERPSLVREPLNRFDSNAIAVHNSRGQQVGHIEGRTAKVLAPLLDSHLVANTLVVVPDGRSSKAGTSFYKLPCQIHLFARPAAADIVREAIDGSGLVLIDPDHIEFSLSGSAFVQEQAKKSGRDVDKLFARVGKEGGSRIEPMEPPEDVVVSDLFEHQKAALGWLVHREESCDLPPFWKEDKAGGYENVLTSQSTKQRPQPLRGGIFADDMGLGKTLTLLSLIARSKARNVGGGKAKGTKRRKIDDTEAGSRTTLVVCPPSVFSSWVTQLEEHTNAGSLKVYMYHGQRTKDKDVLLKYDIVITTYSVLGTEFDQEGSPVNDIEWFRVILDEAHIIKNSAALQTRAVTALNAQRRWVVTGTPIQNSSLDLYPLMAFLKFEPFSVKSYWQSLIQHPLEKGNKAGLSRLQNLLGAISLRRTKETECGSKSVVAIPPKTVVACYIELSAEERECYDQMESEGRNKMMEFGDRDSILRNYSTVLFLILRLRQLCNDVALCPFDIKSWLPANTLEDVSNSPELLKKLASLVADGEDFDCPICLSPPSKTIITSCTHIYCQTCILKILKSASSRCPICRRSLSKEDLFIAPAVQHPDDDDSGSLDSDKPLSSKVQALLELLNRSRNEDPSSKSVVFSQFQKMLVLLEGPLKRAGFKILRLDGTMSVKKRSDVIKQFAVVGPDVPTVLLAGLKAAGAGVNLTAASTVYLFDPWWNPGTEEQAMDRVHRIGQKKAVKVVRLIVKNSIEERVLELQERKKRLISGAFRKKGSSKEEKELRLEELRIMLGFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYP2</accession>
    <name>A0A8I6WYP2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123439312</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971982.1">
        <property type="nucleotide sequence ID" value="XM_045116047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYP2"/>
    <dbReference type="SMR" id="A0A8I6WYP2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229120"/>
    </dbReference>
    <dbReference type="GeneID" id="123439312"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0190000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0190000.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0190000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439312"/>
    <dbReference type="OMA" id="ENDEFYM"/>
    <dbReference type="OrthoDB" id="773737at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15795">
        <property type="entry name" value="PMEI-Pla_a_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034088">
        <property type="entry name" value="Pla_a_1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357:SF8">
        <property type="entry name" value="OS01G0111000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357">
        <property type="entry name" value="OS02G0537100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035176392" description="Pectinesterase inhibitor domain-containing protein" evidence="4">
        <location>
            <begin position="29"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="5">
        <location>
            <begin position="21"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="21116" checksum="ADE78BC6848545C9" modified="2022-05-25" version="1">MAVGTSSSMLGNALLFLLVLSSATDVHGESSSTAATTSPLDYLCGLVGAWYVTPDLCVSVLCVDPSCHSARGWPELAVLATRLTVANATVTKASIQSALAHAKDAKVRKVMQSCLQLYAGAVPRLQWAARSVAARRYNGVPEVLRAAYMDVANECASLAGQVKLPKENDEFFMMAYVADAVVELVQPYGIHLLTRSSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4M9</accession>
    <name>A0A8I6X4M9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Helicase and polymerase-containing protein TEBICHI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123443231</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975488.1">
        <property type="nucleotide sequence ID" value="XM_045119553.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6X4M9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067200"/>
    </dbReference>
    <dbReference type="GeneID" id="123443231"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0053750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0053750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0053750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0067200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443231"/>
    <dbReference type="OrthoDB" id="2320933at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18026">
        <property type="entry name" value="DEXHc_POLQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08638">
        <property type="entry name" value="DNA_pol_A_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000002">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.20:FF:000003">
        <property type="entry name" value="Helicase and polymerase-containing protein TEBICHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.10:FF:000003">
        <property type="entry name" value="Helicase and polymerase-containing protein TEBICHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000968">
        <property type="entry name" value="Helicase and polymerase-containing protein TEBICHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001000">
        <property type="entry name" value="Helicase and polymerase-containing protein TEBICHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.10">
        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046931">
        <property type="entry name" value="HTH_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048960">
        <property type="entry name" value="POLQ-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF62">
        <property type="entry name" value="DNA POLYMERASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20470">
        <property type="entry name" value="HTH_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21099">
        <property type="entry name" value="POLQ_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="498"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="726"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2066" mass="225840" checksum="95E377CFD0C235F2" modified="2022-05-25" version="1">MASGSSRGHVDQFFAAKKRRPSSQHGSPGAAKGSLAGYLVRSPPAAAAACSSAALAGSPSGARRSLAASMNSDIANSAAAMNVDAGNSAAPATDYGDDLEMKRFTMEFLSHYCSDIPSVMMSDTGKGEPDKNQKRGAGNSFLAPCGDRSAKKQCVSRCGADEVPRVKELGNNMPLKWVGNRGASEPLEGFGALQRSSFTPNAAQKIAGFSAAPGETPKSASSLVSPGEDFWNAAMEFADGVSAMAHKGPRRRHCDAAADKSSCAVALGSKTLPRSGNEEFNCENTVGSNPMKQIDTFPNTSELAVTNRQHKNNSPLPVKHLDFFHEDAIQVSGLEGKEKGGTVPGSAQVNQGRPKDSSFYRTENLMRSVDDTKTITFNPDVGSTVMIPGEGLFESKTAGMGHSIDVGVRGSCMIKSDLNQLTHGKAKLPAANSNSCKPNRDSKSKFASQRVEACTPTSSVPLKDHSKLSSWLPQELCAIYMKKGIPQLYPWQVECLLVDGVLENRNLVYCASTSAGKSFVAEVLMLRRILSSQKIAILVLPYVSLCAEKAEHLEQLLAPLGKHVRSYYGNQGGGSLPKDTSVAVCTIEKANSLVNKLLEDGRLSELGIIVIDELHMVGDQHRGYLLELMLTKLRYAAGEGNSESSSGETSGSSSGKAGATHGLQIIGMSATMPNVAAVADWLQAALYQTTFRPVPLEEFIKVGNQIFDKDMNIVRVLPKIADIGGKDPDHIVELCNEVVLEGHSVLLFCSSRKGCESTAKHIAKFLKVANVGSKEVSSEFRDVASAVEALKRCPAGLDPVLEETLPFGVAYHHAGLTVEERDIVETCYRKGLVRVLTATSTLAAGVNLPARRVIFRQPRIGRDFIDGTRYRQMAGRAGRTGIDTKGESILVCRPEEVKRITGIVRSDCPPLQSCLSEDKNGMTHAIMEVVAGGIVQTASDIHRYVRCTLLNSTKSFDDVVKSAQDSLRWLCHKRFVEWNNDTKIYSTTPLGRASFGSSLNPEESLVVLDDLSRAREGFVLASDLHLVYLVTPINVEVEPDWELYYERFMQLSSLEQSVGNRVGVTEPFLMHMAHGAAMPIRGRPKRNTSGGSGVNTLINDQSLRVSKRFYVALMISRLAQEIPVTDVCESFKVARGTIQALQENAGRFASMVSAFCQRLGWQDLEGLVAKFQNRVSFGVRAEIAELTTIPFVKGSRARALYNSGLRTPVTIAEASIPEIAKALFENSWSGQDDSGLRRMQFVIAKKIKNGARRIVLEEAEAARVAAFSAFKSLGVEVPQFTTPLLPASEDSPPREAMVFPSAGHAKCQESVLGARDGDERNICSDYVAQRASTEIVGEDMHPVSTIHIKESQGIANSVNLASMQEASPLSTLPSRNVADRGPVNAHNFPGGFDGFLDQWSSVNEFSFDLHFVKKSSKLSLTIFEILGVAVCWESSPVYYCNFPKDLTPTGNNNSVELWEGFRRRWSRIVGIMQQKSVKKMTWNLKIQIQALKSPCVSCQRLSRLHLDPKTLNNIEVLDSTYVLLPPISVYNGLDICLVAWILWPDEESKTVPNLEKLVKRRLPSEAAAAANRDGRWRNQMHKAAHNGCCRRAAQTRALGSVLMKLLVSQNLSDLIETVEGPLVNVLADMELWGVGADMDACLRARHIIITRLKELDKEAYKLVGKSFSLNANADIADILFTHLKLPVPKGCEKGKLHPSTDKQCLDHLRDLHPIVSVIKEHRTLAKLLNGTLGSICSRAKLCIQSQRYIIHGNWLQTSTATGRLSMEEPNLQTVEHMVNFTTGKNDKDFTSMSMVDRHEINAREFFVPTQDNWLLVTADYSQIELRFMAHFSKDPVLIELLSKPDVDVFTMIASRWSGKEESLVSSKDRDNTKRFIYGILYGMGSNSLAEQLECSPAEAAQKIQSFKRFFPGVSSWLQQAVASCRQKGYVETLMGRRRFLTKITAGNSREKAQAQRQAVNSICQGSAADIIKVAMLKVHSVITNGSNTVDSMDGLTQNFSQIRGRCHLLLQVHDELVLEVDPSMVAEAVRLLQISMETAASLLVPLRAKVKVGKTWGSLEPFHPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7B8</accession>
    <name>A0A8I6X7B8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7B8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0320780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0320780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0320780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0267710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0267710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0267710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0320780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0320780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0320780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0320780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="13506" checksum="40C1AA689E3CCE0C" modified="2022-05-25" version="1">MNAEHIWSIVSEFFMIRKIQCFDDISSMWKSKKKRIMLNMITATSLWSIWTLRNDLCFQGRSWKGLGCGLAKLKGNLQKWTVLCDATQVEVLRRFALLLDKHRGELLRITWRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XC78</accession>
    <name>A0A8I6XC78_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981485.1">
        <property type="nucleotide sequence ID" value="XM_045125550.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981486.1">
        <property type="nucleotide sequence ID" value="XM_045125551.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981487.1">
        <property type="nucleotide sequence ID" value="XM_045125552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XC78"/>
    <dbReference type="SMR" id="A0A8I6XC78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0374560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0374560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0374560"/>
    </dbReference>
    <dbReference type="GeneID" id="123448601"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0311820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0311820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0311820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0374560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0374560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0374560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448601"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF6">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0374560.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="59279" checksum="6C13143FC025C562" modified="2022-05-25" version="1">MPPLPRIFASVRHICSSSASASELAPVPTAVSDAASRAAVAVLRAPRFEPRLLSLVPRQLLLEPSCVRQTLSRLLPSPEPSLRFLLLLASHLPAPADAPAPSPHLPGVDAFLLSLQPHLAADAAALLASHLGLHPSLPALNAASRAALRAARPDLVFRLFSTFSSSPAFPGNADTVACLVRACAAECRPLDGLRLLRDAARRGSPPSSAAAADLITSFVAIGNFTKVSETLHLMLSAGCPPATFVYQRVIAGLFAHGMGGEALRVFNEIKLRGYAIDVVTYTTALTGLCKMGHIANAQQMWDEMVDKGIQPSEYAYCSFLDYYCEAGDFGMALKVYEEMLEMGFKESTVSCNIIARGFCVHRRVDEAVGVFDGMDTKGIQHDVITYNTLIQGLCKVGRLAEAIGMYQRLLSSGLEPSVQTFTPLIDTLCEDGQVDHAVELLTLMQAKGLEPLERSNDSVINGFCKVGRADDGMAWLASMLKNNLKPQEQTLNYLLESLSKSERMDDALLILNTMFKAGYELGSLASAILVDKLCTGNLPCPQKLEHILVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCX4</accession>
    <name>A0A8I6XCX4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="SMR" id="A0A8I6XCX4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0313740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313740"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF73">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="12">
        <location>
            <begin position="129"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1425" mass="159903" checksum="3B3E5B4B4D08CD82" modified="2022-05-25" version="1">MEEASLEAAIGWLVQAIYAILLAGGELDAWIRRAGLADDIERLRSEVEGVEMVVSAVKGRAIGNKPLARSLARIRELLYGAEDLVDELDYLRLQQQLELEGGTRHDHDETDAHGSLQVERSTESSSVDRLRSESWGHFDIEEFAGNGGPVRAICKHCGVVVECTTDKGTPILRKHLKSKSCKKKRGASDLSSTGDATASTPSTATDGISSTRNMRGVGVEEPKHGTTSDTYTWDKNNFSNRIKDITHQLQGIRGDVREALMILGSDSSASSNHHVEHPHRRTSSLVQGKVYGRDHEKGKIIQLIKGHKSSSTVTVLTVLGIGGVGKTTLAQLVYNDPSVESQFDHRIWISVSKKFDETRISREILDFVSQGTHEGMYSFARLQEVLKGYIKSKRVLLILDDVWDDMNDCQWNQLFALFKSGNAKGNIILVTTRKPTVAKRRGTVEPINLHGLQNDVFWILFKACVFGDENCEVEGSLKILGRQIIEKLNGNPLAAETAGALLREHLTIDHWSNILKSEDWKSLQLRRGIMPTLKLCYDQLPYHLQQCFSYCAIFPCDYRFLAEEIVRIWISLGFVKCSHSSMRLDEIGRLYLADLVNLGFFQQIEGVHSSSSQTTYVMYGLMHDFARVISRTECATLNALECNEISSTVQHLSIVTDPIYWHKEKFEEILRKTVKNLRSLVLIGHFDSQLFQQILEKAHNLRLLQVSATVRSHSLPCGLVNPTHLRYLNLQVSHVWQGDLPQALSKFLHLQVLEVGLCRGTSFASGQMVNMEGCGEKLIFLSLERLLFLTTLKLTNMRKIRQVSVPSLQELILVEMPELERCTCTSLGALNSSLRVLEIRSCPALKVFDLFEKGHNFAEQQLMFKDRNFKTNWKPWLPGLRVLTVINCPHLLMPCLLPHSTTVSSLHIDGVPTLLKLKGSSREKLEISPNSRSGVNTDETMRLDGEILAFHNWGDLKSLHISFCGNLTSISFQGLGELFSLEDLHIVGCTKLFSSDVRPELTDEYATTGPYESLRCLRSLYIRSSGISGKWLSLMLQHAQILKELSLYDCPQFTHLRIEEEENNQPDIISASEASSSSGPDGLLCIPLNLTSSLEKIDISGSPYLRFDGNKEGFAGFTSLEELRILCCSGLLLSLVHNKGNYVQANRTWLLPQSLGQVVISSYAYETLSLCFLGNLTCLRKFQVLESPGLKSLQLHSCTSLEDLRIHKCKQLATVKGMQSLVNLMNLEVQENPSLVSLELHSCRSLEGLRIEECESLIALEGLQSLVNLKNLALLNSPGLPPYVERLSGHYELCPRLESLHIDDLSLLNTSLCIGLTCLQSLQLEKLERGGTRLTDEQERALLLLRSLQYLEFQDCLNLVHLPVRLHSFPSMKRLMICRCEYIPRLPKKGLPSSLECLVIYDCSTELREQCELLTRGKLIVMMND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDC8</accession>
    <name>A0A8I6XDC8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123430929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044970692.1">
        <property type="nucleotide sequence ID" value="XM_045114757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDC8"/>
    <dbReference type="SMR" id="A0A8I6XDC8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0200430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0200430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0200430"/>
    </dbReference>
    <dbReference type="GeneID" id="123430929"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0166490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0166490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0166490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0200430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0200430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0200430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430929"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="96"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0200430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="40748" checksum="A9E95B89F4AD667C" modified="2022-05-25" version="1">MSHSLRTLFSLRPSMADAPVASVGPVPFKDVDADSGSGSSVLEQLPEEHADLVSGLPCAPRPNGLSNLRCYEGFWLPEWSVPVAVALQRRFQPRRDDVIVASFPKCGTTWVNALTFATMARRAHPAAGAGHPLLRLNPHQCVPFLEALFRSRRGEAKLEALPSPRLMNTHMPLRMMPPADSAGGCKVVYVCREPKDMVVSLWHYLRRLQPDLPLADVSESVCGGAAMYGPVWDHILGYWSASTARPDSVLFLRYEELLRDPAKHVRKLARFVGLPFSGAEEEAGVVDDIVKLCSIGHLKTLEANKTGHVDPIFPIPREALFRKGVAGDWVNYMTPEMARRIDEIVADKFHTTGLTFQEWLTHADSIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEW2</accession>
    <name>A0A8I6XEW2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEW2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF8">
        <property type="entry name" value="PROTEIN ENCORE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUZ" evidence="2">
        <location>
            <begin position="68"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="42204" checksum="46FAC2D1A9085971" modified="2022-05-25" version="1">MEQDILKFLRDPGQTQFEFQGLPTSYLRLAAHRLAQHYFLISIALPDNSLPDGTSSRIILRKTSAECRLPAVRLADIPVNLPQEETSAVATKVAIKQRPQKNHHGGAGAGANSSRGNLQKSVEERKEEYNRARARIFNNSSGSSSPVDGRPADEVVLPNTLHRSTSLELNPNTRYGELAEATLERSLTSTASSSRSNNRSRIDKEPPVNRGRQGNRVAIFRDRDSDRKDPDYDRSYDRYMQRFDPGFGFNGGAYTIQPLYAPAVTYNTEFPQLGSPQMSPVPVEQQQPHPMAQHMPGPWSPAQSPNAVGYRPPDGVMPPYSPGQAGAPVRSSVFMHASQQYAMPSRPGVTFVHPQDSMRPFAQTHHQQQSEASLRLARPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XF50</accession>
    <name>A0A8I6XF50_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XF50"/>
    <dbReference type="SMR" id="A0A8I6XF50"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0209730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0209730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0209730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0174450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0174450.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0174450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0209730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0209730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0209730"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31718:SF64">
        <property type="entry name" value="OS10G0361900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31718">
        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035219789" description="PLAT domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="21"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0209730.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="16527" checksum="51EA9B923C4F40AA" modified="2022-05-25" version="1">MKILLVLFLLALTQSTAYAQCEFVILVTTGNKEDAGTDARISLSVSTANGKKLVIESLKPWGQKGHNNFERGHTDTFEGRGKCLPSKPCRMLLESNGKGNKPGWFVDKVEFSQIEPNLSVKQKTFKVNRWLARDVSPGTLFVVVDDCPK</sequence>
</entry>
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    <accession>A0A8I6XG75</accession>
    <name>A0A8I6XG75_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG75"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191360.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0158560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0158560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0158560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0191360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="2"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8745" checksum="EF0E261D94256B27" modified="2022-05-25" version="1">MSVAYRILLTVPVIIASAERSFSKLKLLKNYLRSTMLQDRLDGLDMCCIEKDILDNVDLDCALNDFASRNARRNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHI7</accession>
    <name>A0A8I6XHI7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123427828</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 60S subunit of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967898.1">
        <property type="nucleotide sequence ID" value="XM_045111963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHI7"/>
    <dbReference type="SMR" id="A0A8I6XHI7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195920"/>
    </dbReference>
    <dbReference type="GeneID" id="123427828"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0162570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0162570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0162570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0195920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427828"/>
    <dbReference type="OrthoDB" id="1649877at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.50:FF:000001">
        <property type="entry name" value="ubiquitin-60S ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="142" mass="16337" checksum="680230DCB5452EF5" modified="2022-05-25" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGSRGQYPKNLEPNLRILAQKYNENKLVCRRCYARLPLRATNCRKKKCGHSNQIREKKRFMSKFGSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJY6</accession>
    <name>A0A8I6XJY6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123442866</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044974971.1">
        <property type="nucleotide sequence ID" value="XM_045119036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJY6"/>
    <dbReference type="SMR" id="A0A8I6XJY6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235770"/>
    </dbReference>
    <dbReference type="GeneID" id="123442866"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195630.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0235770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442866"/>
    <dbReference type="OMA" id="FSFLYHG"/>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000811">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240092" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="29"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="354"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="676" mass="74986" checksum="B101DBD4464AFE88" modified="2022-05-25" version="1">MRGMAPREHTFFLLHAIPLLVLLPLAASTTSNDSFNFLYQGFSGVNLTLDGNAMVTPDGLLELTNDTINLGHAFYPTPLSLRGSPNGTVRSFSLSFAFAILSVHDGISADGMAFFVAPTKNLSNTWAQYMGLLNSGNNGNASNHMFAVELDTTQNEEFQDMDNNHVGIDINSLESLQAHQTGYYDDGSGSFNNLTLISGKAMQVWADYDGVSTQIDVFLAPLGFAKPVRPLLSSPYNLSTVLREPSYIGFAATTGAISTIHCVLGWSFAINGPAPAIDISKLPKLPRLGPEPRSKVLEITLPIATATFVLVVGTVIVLFLRKRFRYRELREDWEVDFGPHRFSFKDLFHATQGFKEKNLLGVGGFGKVYKGTLPKSKLKVAVKRVSHESRQGMKEFIAEVVSIGRLRHRNLVPLLGYCRRKGELLLVYDYMSNGSLNQYLYCEDGKPSLSWEERFHIIKGVAFGLFYLHEEWEKVVLHRDVKPSNVLLDSDMNARLGDFGLSRLYDHGTDPQTTHMVGTMGYLAPEFVRTGKASALTDVFAFGIFLLEVTSGQRPIKQNPFGNKHTLVDWVIERWHNGSLMDTVDQRLQGHYDVDDASLVLKLGLLCSHAFTSARPTMRQVMQYLEGDTPLPELTPAHFSFTMQVLTQNRGLESANLQYPQLSTSFATFSGLSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK03</accession>
    <name>A0A8I6XK03_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK03"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0369690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0369690.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0369690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0446390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158836" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10657" checksum="CA179106DD0535D1" modified="2022-05-25" version="1">MNQFIYDMWHFLVRFLTNLLECYSLPRQICVMYKHDQPNSSPLFITDNMQNNKKCGEDNTICGQYVGKFIIESIELVNLILGVLQPLLQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK97</accession>
    <name>A0A8I6XK97_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK97"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0430700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0430700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0430700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0430700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0430700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0430700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0430700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="104" mass="11592" checksum="FFB30D612E811489" modified="2022-05-25" version="1">MALLTAEVTWLRWLLQDFGVSVTTPTLLLSDSTGSISIARDPVKHELTKHIGVDAFYVRAALQDQVVALQYVPSELQLADFLTKAQTRAQHGFYLFKLSVVHPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLJ8</accession>
    <name>A0A8I6XLJ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nicotianamine synthase</fullName>
            <ecNumber evidence="2">2.5.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-thioadenosine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16481"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58249"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nicotianamine synthase (NAS)-like family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.43" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044980037.1">
        <property type="nucleotide sequence ID" value="XM_045124102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLJ8"/>
    <dbReference type="SMR" id="A0A8I6XLJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415050"/>
    </dbReference>
    <dbReference type="GeneID" id="123447493"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0415050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447493"/>
    <dbReference type="OrthoDB" id="586689at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030410">
        <property type="term" value="F:nicotianamine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030418">
        <property type="term" value="P:nicotianamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004298">
        <property type="entry name" value="Nicotian_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266:SF29">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03059">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51142">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="335" mass="35620" checksum="05C8057D701C0417" modified="2022-05-25" version="1">MAAQNNQEVDALVEKITGLHAAIAKLPSLSPSPDVDALFTELVTACVPPSPVDVTKLGPEAQEMREGLIRLCSEAEGKLEAHYSDMLAAFDKPLDHLGMFPYYNNYINLSKLEYELLARYVPGGIAPARVAFIGSGPLPFSSFVLAARHLPDTMFDNYDLCGAANDRASKLFRADADVGARMSFHTADVADLAGELAKYDVVFLAALVGMAAEDKAKVIAHLGAHMADGAALVVRSAHGARGFLYPIVDPQDIGRGGFEVLAVCHPDDDVVNSVIIAQKSKDVHADGLGSGRGAGGQYARGTVPVVSPPCRFGEMVADVTQNHKRDEFANAEVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLW5</accession>
    <name>A0A8I6XLW5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0253720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326:SF14">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326">
        <property type="entry name" value="OS05G0543800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3615" evidence="2">
        <location>
            <begin position="66"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="185" mass="21901" checksum="8867DAC4FC7E44AD" modified="2022-05-25" version="1">MWKNHPKKDNKPAEQPVMQSWIGRAAEQFKSTTTDPAILQARYRFERFMEEKETKKNGMKWMQKEALRAYENYKSADKNLEYELVMIHSQCLIYDTFSKSYHHYNFTMRTRRSRLKHWNYQQYFAESKSVEGKTQYFCIPLQPNEHGHCFGCRKVGVSLRHPENGGYEKGNSGSGFPFDTDESAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLY3</accession>
    <name>A0A8I6XLY3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123439767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972380.1">
        <property type="nucleotide sequence ID" value="XM_045116445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLY3"/>
    <dbReference type="SMR" id="A0A8I6XLY3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254240"/>
    </dbReference>
    <dbReference type="GeneID" id="123439767"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0211110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0211110.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0211110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0254240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439767"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001640">
        <property type="entry name" value="Pentatricopeptide repeat protein PPR986-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003690">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF432">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="265"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254240.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54681" checksum="9AEEA71A6DC64B7E" modified="2022-05-25" version="1">MPPLSFALHRAFSLLNRLDSPRHLLQAHAFLLTRGGHRNARLLSALLLASLRLPLRLHALALLRRIHPSVSLNAAARIPHLRGTLGPQLHSIVVRAGLASDAHVSASLIQAYFSCACAASARSVFDETVHKDIFCWNVTISGYVKSGNLDCARQLFDVMPQRNVVSWTTLIGAYAQMKRPAQAVEMFRRMQVEEAIEPDGIAMLSVLSACGDLGAVDLGEWVHRFIVKRGLCWHIPLMNAIIDMYLKCGCVAKAVEVFEGMGHKSIVTWTTLVAGFALHGLGSEAVDMFRRMERENVAPNDITFLAILSACSHIGLTDLGRWYFQIMVSHYRIKPRVEHYGCMVDLLGRAGCLMEARDLVKDMPFKANAAIWGALLAAARTHADAELGEQALVHLIELEPHNSGNYILLSNIYAEQERWDEVSKLRKAMRDGGLRNVPGASSIEVGGVVHEFTSRDGSHPCLHKIHKILREITADMKSIGYVAVLPEAPHDIEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMK7</accession>
    <name>A0A8I6XMK7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123443843</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-8 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976308.1">
        <property type="nucleotide sequence ID" value="XM_045120373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMK7"/>
    <dbReference type="SMR" id="A0A8I6XMK7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270270"/>
    </dbReference>
    <dbReference type="GeneID" id="123443843"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0224030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0224030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0224030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0270270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443843"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061673">
        <property type="term" value="C:mitotic spindle astral microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000054">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="202"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="731"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="294"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="769" mass="84360" checksum="2E274A41037DB191" modified="2022-05-25" version="1">MPVSTRSQASATKGGDLGAHPWSSSAARVPMSAPGRRASLLTNTHHGLKEKMRELNLFYEQHKQILASSQAGAAMRRSRTIPHANVGEDRDENAGEGEGAKRHRDAFAPLPEAAVLRENMGHPDARPPSKNGNVVVFSRPPEPREKENVAHAANVMSCPVKKGAPALPALQARKLSLGGGIGGKLKALGEMGAANADSTGSRILVFVRLRPMSRKEKEAGSNTCVKIVNKKDVYLTELASENDYLRLKRVRGRHFCFDASFPDSTAQAEVYSTSTADLVEGVLQGRNGTVFCYGATGAGKTYTMLGTMENPGVMVLAIKDLFLKVRQRSYDGSHSIQLSYLEVYNETVRDLLSPGRPLLLREDKQQGTVAAGLTQYRAYSTDEVMELLQQGNQNRTTEPTRVNETSSRSHAILQVVVEYRYMDGTSVVTRVGKLSLIDLAGSERAIATDQRSQRSLEGANINRSLLALSSCINALVEGKKHVPYRNSKLTQLLKDSLGGSCNTVMIANISPSNLSFGETQNTLHWADRAKEIKTKPLTAGNEEVFKAPDSDTEQAKLVLELQKENSELRQQVVKQQHKLLTVQAQSLAPNGSPQQAAPSPHITTTPCSTQRKAKRSILDGSCFSTPNSKRPADIGQVRDLQRKVKTLESEIEKMKREHFLQLKQRDEFIRGLINRKNSNDPESATAERRVIRRASLRKAEKDAGELKSPSHRFTSPIPTAKKRTFWDFGGESPSVLAGNGRKTRSHVAAETPTRAPSMLLQPGFTRQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWV3</accession>
    <name>A0A8I6XWV3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3741 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123452460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044985046.1">
        <property type="nucleotide sequence ID" value="XM_045129111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWV3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513980"/>
    </dbReference>
    <dbReference type="GeneID" id="123452460"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0427090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0427090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0427090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0513980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452460"/>
    <dbReference type="OMA" id="DDERITH"/>
    <dbReference type="OrthoDB" id="761625at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032795">
        <property type="entry name" value="DUF3741-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34282:SF1">
        <property type="entry name" value="DUF3741 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34282">
        <property type="entry name" value="OS01G0228800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14383">
        <property type="entry name" value="VARLMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="2">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="760"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1165" mass="126983" checksum="720A273568DBE23F" modified="2022-05-25" version="1">MAASARVPDLGSDFAQKMLQDLRRRRERLGFGSPSAQQQQQRTASTAAATATASRDACSNSQRPAPSQRPQQAAAAPRSTRPEATTTPRQPSTSNAVLASAARPRRVRPVAAADADARAIVPFQGGGGGAGGGKPEHAVVASGVDVQMAALALALSDGGKLRNIEVVARNGSVFFRQPDAARLGVGGGGGYLLPPPPSGGDTGAHAGEVAIGVQDLNDMLMAAYSSGGRRRPDDEARKKLFRGSMDMEEALSMLVMLQDASRYMEGSGSGLKGKENRKSSPAARSARIEEIVDEDSDAEHGKHASMQMVVHDKFQSHESADSRSVIQSSPGSSKINSASGGEKDGSKVRMPSVIAKLMGLENLPSSTTTTTAAERKGTERFVKPGAAAPRMEVRGNAMDRKLPIRIIASEKGQHKIVLAGEWKNGMTNFGESEMGAAALSNSSSHPATGNSKQGRLTMREVLRKMVAAERGADERQVADERIIHEDKTAAEEIKLQNSVDVGCRADSGKRMDFLKRFRKNSDNRPVKEEKQTAQVKKAEVGKKQATGMRRLLGRDGEAKSRKAREKVNKENLATAETKAAGKNVKADQMRKQAQSKHVERQITARKPQNRRETQRETPSRKTENKKSVMSEAGHMRKKPEYNLVTQQDNEEHAKEHDSFSKPADSTHGEGGLSEPLAIVVRGSSIAGAASLDQPLQKITEGSTEKATETPREPQNCRGKQSETPSRKLENKKLSLSEAGHMRKKLDTVVAQQENEVDANVNCTISSSKPADSTKGEGGLSEPSTMVVRDSTTAEAASLDKPLQKITEGTSDPTVPAESVVQASEDLKFLDQSAVAEIDDERMNHTPSETTQIPEIFAEEEQQQEQEMIVKEQLTEDLKFLNQSTIPETNDEQINHTPDEATELPETFAEEEQQQQQQERDQQMIVIPETFSEMEEQQQQMIVKEQLTDGSDHTTGSISSENLQQDQETHVVTCDSLTENQLLLMRLLVKDRYLLETAKSIVRVDAPVSFMDDVASAPNWSDKGNELLSDVAREVTRRKGKRSEAMEEVSVARGTNPRLRYLDDLVRELDGDVESLDMSKSKSARHQGDNGAAENLRRILEGDIQNEHPDANSTWDFGWNRVWELPLEKADVVRDLEKNILGGIITDVARDLIGVSVRHGCCPCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XY94</accession>
    <name>A0A8I6XY94_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Indole-3-acetic acid-amido synthetase GH3.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IAA-amido conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972850.1">
        <property type="nucleotide sequence ID" value="XM_045116915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XY94"/>
    <dbReference type="SMR" id="A0A8I6XY94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294170"/>
    </dbReference>
    <dbReference type="GeneID" id="123440343"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0244730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0244730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0244730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0294170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440343"/>
    <dbReference type="OrthoDB" id="10004661at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004993">
        <property type="entry name" value="GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055378">
        <property type="entry name" value="GH3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055377">
        <property type="entry name" value="GH3_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901:SF9">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03321">
        <property type="entry name" value="GH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23572">
        <property type="entry name" value="GH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23571">
        <property type="entry name" value="GH3_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH3 middle" evidence="3">
        <location>
            <begin position="363"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="GH3 C-terminal" evidence="4">
        <location>
            <begin position="455"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="67949" checksum="4B25F48DDA4FBB59" modified="2022-05-25" version="1">MPEAPSALPAPASGYAPGAHREALDFIEHVTANAGKVQQRLLAEILAQNATAEYLRRHGVSCSSSPLDAVDVFRRCVPLVTYEDLQPDILRIANGDTSPILSGKPISEFLTSSGTSGGERKLMPTIADELDRRSLLYSLLMPVMSQSLPGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPHDPYTAYTSPNEAILCVDSYQSMYAQLLCGLVHRTDVLRVGAVFASGFLRAIRFLEKHWRRLCHDIRTGELDPEITDRAVRDAVGRLLCANPALASEIEAECSKPSWEGIIRRLWPRTKYIDVIVTGAMSQYIQTLEFYGGGLPLICTMYASSECYFGLNLNPMCKPGDVAYTLVPTMCYFEFLPVHCSNANAEPSHHDLVDLVDVKLGHDYELVVTTFSGLCRYRVGDVLRVAGFKNEAPMFSFVRRRNVALSIDSDKTDETELHTAVSSAMQHLAPFGASLVEYTSYADTAAIPGHYVLFWELREGSTPVPASVFEECCLSVEEALNSVYRQCRACDRSIGPLEIRVVSEGTFEKLMDYALSQGASINQYKAPRCVRPGPVVELLDARMQASYFSPKCPKWSPGNKQWNTSNKELLSIGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZD5</accession>
    <name>A0A8I6XZD5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123402941</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
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    <reference evidence="8" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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        <source>
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    </evidence>
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        <source>
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</entry>
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        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
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    <reference evidence="4" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540320.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14888" checksum="F7F31E1F6299ABE0" modified="2022-05-25" version="1">MYSAMVLISVPVGPRPVYDSDVDVVEPEMPIDPIVEKLHLVEQENVGLKERLKKTEERLEKTEREKMTLEKVLAGVVDGHKSEMDAMRLKIRKIRKYAISKEAWYHYAVGSIVTFVAILIAFVVVFKCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2Y4</accession>
    <name>A0A8I6Y2Y4_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2Y4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0598430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="27049" checksum="045EE32690FC1779" modified="2022-05-25" version="1">MATFIPVKFTEFCQPDATMPFGKELVQITKDLRIALPTITGKPTSSYPEDSRYRIKVHVPGRTFEPRTEPVDFKFIAPNWILGRDMAIHCALGRIKEEYKGCPISPHLFTIFRRNEDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLRELELEEAAREAHYEHELEVKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPITMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y315</accession>
    <name>A0A8I6Y315_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123446786</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979264.1">
        <property type="nucleotide sequence ID" value="XM_045123329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y315"/>
    <dbReference type="SMR" id="A0A8I6Y315"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396280"/>
    </dbReference>
    <dbReference type="GeneID" id="123446786"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446786"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000509">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g16390, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001363">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein ATP4 homolog, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001282">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein ATP4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF12">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ATP4 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="365"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="438"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="10">
        <location>
            <begin position="585"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396280.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="683" mass="74621" checksum="38C7E503ECFC5D0C" modified="2022-05-25" version="1">MSSLPICPSSPSSFLSWPHRPISLSFQPRNHSSSASPAAAHVAVQDSPPPSDPGQNPKISSTARFLWVNPNSPRAAELARARAGSGRRARLAAAAAALAACEPADAPVAAALEAAFPEAPSEQDAAIVLNTAAGNPATAVLALRWFLENAEVRSKVILYNVVLKVLRKRRRWSETEALWDAMLRDGVQPDNTTFSTVISCARACGPPGKAVEWFEKMPETGCSPDMLTYSVMIDAYGRAGDAEMALRLYDRARSERCQLDPVICATVIKVHSTNSNFDGALNVFEEMKAVGVKPNLVVYNTVLDAMGRAMRPWVVKTIHREMVSQKVQPNRATYCCLLQAYTRARYGEDAMIVYRKMKDEVMDIDVVLYNMLLSMCADIGYVEEAEEIFRDMKSSMDTKCKPDSWTYSSMVTLYSCTGNVPGAEAILKEMAEAGFKPNIFILTSLIRCYGKAGCTDDVVRSFGMLEDLKITPDDRFCGCLLTVAADTPVEELGKVVECIDRSNAQLGTVVKLLVDRKASTESFKEAARGILSGVRGVVKMPYCNCLMDLCVNLGQMEKACALLDAALQLGIYSNVQTRTQTQWSLHLRGLSVGAALTTLHVWMNDLYTTLQSGEELPPLLGIHTGEGRNMYSDKGLASVFESHLKELDAPFHSAPDKAGWFLTTSVAAKHWLEAKKSSELVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3C0</accession>
    <name>A0A8I6Y3C0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123402221</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044952049.1">
        <property type="nucleotide sequence ID" value="XM_045096114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3C0"/>
    <dbReference type="SMR" id="A0A8I6Y3C0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0602560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0602560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0602560"/>
    </dbReference>
    <dbReference type="GeneID" id="123402221"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0499580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0499580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0499580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0602560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0602560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0602560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402221"/>
    <dbReference type="OMA" id="LGHLFMT"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF116">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035259210" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="6"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0602560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="47823" checksum="37FBD1F96C989814" modified="2022-05-25" version="1">MKDLVGLGHLFVVAFLFHFSSFMVLPAITDVTMEAVCPGRDECSVAIYLSGFQNAITGLGALVVTPIVGNLSDKYGRKALMTLPVTVAILPLFILACNRSAVYFYVYYVVKILAGIFCEGSIHCLSLAYVADHVGTKRRAAAFGLLSGVSAAGFLSGTLTARFLATSSTFQVAAAVGTAGAIYLRAFVPDSGAAVSFDDDACDSLLQDSSCSSAASSSASSDEELSPRLPPYKGVLPSPSDMVALLTGSLTLSTAAMITFFYSLGEHGLQTALLYYLKAQFGYSKDEFANLMLIAGAAGMISQLTVMPIFAPIVGEEMLLIVGLLGGCTHVFLYGIAWSYWVPYFAAAFIILSAFVHPSIRTNVSKSVGSNEQGIAQGCISGISSFASILAPLVFTPLTAWFLSETTPFNFKGFSIMVAGFCTLIAFVISIRMRAARCGVSEKVSLVQHEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5P6</accession>
    <name>A0A8I6Y5P6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 17">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="3 17">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123402556</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 17"/>
    <dbReference type="RefSeq" id="XP_044952412.1">
        <property type="nucleotide sequence ID" value="XM_045096477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5P6"/>
    <dbReference type="SMR" id="A0A8I6Y5P6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624620"/>
    </dbReference>
    <dbReference type="GeneID" id="123402556"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0518390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0518390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0518390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0624620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402556"/>
    <dbReference type="OrthoDB" id="10036721at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000005">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000016">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000929">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="18">
        <location>
            <begin position="325"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="729" mass="80620" checksum="3DB2B2AFE73941C4" modified="2022-05-25" version="1">MSGWDEGAVFYSDQAQFPQGDPAAADITRHSALRKFKEFLRGFTGPTGDFPYRESLVHNRDHVTVAIEDLDAFDAELADRIRKAPADYLPLFETAGSEVLASLRSKVAGETGEMEEPVTGDVQIFLSSKENCVSMRSIGADYMSKLVKIAGIAIAASRVKAKATHVTLLCKNCRSVRTVPCRPGLGGAIVPRSCDHVPQPGEEPCPLDPWIAVPDKSKYVDLQTLKLQENPEDVPTGELPRNVLLSVDRHLVQTIVPGTRLTVVGIYSVFQASGTNNQKGAVGVKQPYIRIVGLEQSRDDNTNGPSHFTLDEEMEFKEFAQRPDAYSKICSMIGPSIYGHGDVKKAIACLLFGGSKKRLPDGARLRGDIHALLLGDPSTAKSQFLKFVEKTAPIAVYTSGKGSSAAGLTASVTRDSNSREFYLEGGAMVLADGGVVCIDEFDKMRPEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPISGRYDDLKTAQDNIDLQTTILSRFDLIFIVKDVRMYEQDKRIANHIIKVHASGAATQANKNADVNEGENWLKRYVEYCRNTCRPRLSEKAAEMLQNKYVEIRQKMRQQSHETGRAAAIPITVRQLEAIIRLSESLAKMRLTSVATPEHIEEAFRLFNVSTVDAARSGINEHLNLSPEIANEIKQAEAQIKRRMGIGSHISERRLLDELNRMGMNESIVRRALVIMHQRDEVEYKRERHVIVRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5X9</accession>
    <name>A0A8I6Y5X9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sphingomyelin synthase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5X9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0552500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0552500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0552500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0665940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF48">
        <property type="entry name" value="OS02G0543100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="10">
        <location>
            <begin position="287"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="439" mass="49207" checksum="E316EF7517B35D5F" modified="2022-05-25" version="1">MARPKAERPVPPPACRRPLAVGGLGLAAAAYVGVDYLLSRTSPSLNGRLRPAIWAALALATAARAPFYRRWDAEIRAAPWFLAAMLLMFAALLCEAISVRFVSSVLGLRWHRSTAPLPDTGQWLLLALNERLPRTVVDLLRAHIISLHHYLMLFIMLGFSALFDCIKGPGLGIGTRYMFVMAVGRFLRTITFVSTILPSARPWCAAGRYKIPDHPHAWAQKYYAPYASDPRAIRRVILVDMPYATVQNYTDHYRPDWAYMSFLVDILRPSAGEGPSWYHLVNKAGGGCNDLMYSGHMFVAVLTAMAWTEAYGGWISVVIWFLVLHSAQREIRERYHYSADCIVAIYVGILLWRVTGFIWSTRDSDQARRLSKLDEVQNRLFHAAKDSDMDEIRGLLNEVELAGQSKNTFSQGVILCFAASMITFTLLFVLLAFSLTSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9A2</accession>
    <name>A0A8I6Y9A2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9A2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0632190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0632190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0632190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0632190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0632190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0632190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0632190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="122" mass="14379" checksum="556D29920A1F71E7" modified="2022-05-25" version="1">MIWKAWTPPKVRFFLWLAQQDRCWTADRLARRGLHHHPRCLLCDQAFETIHHIIIACPFTQQIWHEVLAWFRLPVGCPNQEDSLLDWWKPATQHPPPTAERDGIHHPAPTLDGMEAPQQLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9J0</accession>
    <name>A0A8I6Y9J0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9J0"/>
    <dbReference type="SMR" id="A0A8I6Y9J0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0634400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0634400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0634400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0527110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0527110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0527110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0634400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0634400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0634400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.280:FF:000003">
        <property type="entry name" value="Kinesin-like protein KIN-10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF908">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12836">
        <property type="entry name" value="HHH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="24"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="511"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0634400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1015" mass="109280" checksum="A6BB53F2C3A884E0" modified="2022-05-25" version="1">MEEEAAVDLISMEEEAVTSGGDGGVRVVARICPAPTAPAAGFQVAASRGRRPRASGPGAGAVLSFTPAAAAPTPSSSSQGRAAGGQRKEEHRLDWCYLHDDTNHQVFLHELHPILHHQLHQNQNTNAGSSNACVVACGAAAAKDHLFKGSQEQPGLVTIAMEEILGFAASIGGSVRVSFYQVVQDTHVLDLLEPKEQEVLVLEDAQGKTHLKGLSKVHVRSIDDFVRLGYFDDNQDKQQQQLAKPFSTQQQPAKSSSTQLPTRGHQGLIIHVSTSDQDGKERAVAKINFLSLTDYVDPKHKIGSGGAALSSGNKSMYTLMNVVQALNSNQSFVPYRQSKVTRILQDSLCKTSGAVVIACLEEVSCQDAVSTLSLAARSSQVATQVANEQCRRSAIITSFKRADANLSAVAKSSSRPILSSMNLPNPVVEKQDRPQWNMSAVKASRTPVPNKRSRPIMHSAKKSENSLATPIKITQKDATPTMSGRSQPIMHSAKKSENSLATPIKITQKDATPTMSGRSQPIMHSAKKPESTVSPPTKMKQKDGKPAMSGRSQVIMRSAKKTDSSLSTSTKWKQKDAKPTMSGSALLCPSTNSAKEDIAPATVTKVEEVLSSQGVEIDAPSADEGFDKTSDALDTVPSEVQKVVSSGMAIDAPSTDEGFDKTSDALDTASSEIHKVVSSGMEEEDYSSSALDAASSCTIDLGEICSPNIPDALVEKTLVKTIMNTPKISDKLREISNSLKLLNARPLSVMPQKIAMEKTQEQGMEKTQKVAMEPAQEVGMKTAQEVDMETTQEVSKERTQEVGMETTQEVGMETTQEVAVETTQAVGVETTQEVVIETTQEVAVETTQEVAVETTEEVSINTREEVAIKTTQEVAVETTKEVVMENVQGVTNIDAAAEPKTPALHLKLEQAADTKAPGIHLKFEQAADHPASSFKTPSTGIKRSIVQECMNFLNSANKEQLKSLKGIGEKRANYIIKLREHSPELFKGIDDLRDVIGMNKREINNMMSGIITDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAF0</accession>
    <name>A0A8I6YAF0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123396012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946974.1">
        <property type="nucleotide sequence ID" value="XM_045091039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAF0"/>
    <dbReference type="SMR" id="A0A8I6YAF0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493440"/>
    </dbReference>
    <dbReference type="GeneID" id="123396012"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0408840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0408840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0408840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0493440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396012"/>
    <dbReference type="OMA" id="HEHTLES"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="199"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="41612" checksum="8DA273202C26976F" modified="2022-05-25" version="1">MVHADQEKLVQVVAADVGLVAPPSRYVLREENRPTTVAKQAKLVIPIVDVSRLAMPDDVEESAKLRSALQSWGLFVVTGHGMPTELLDEILEATRKFFHLPPEEKQKCGNVIDGVSFQNEGYGIDRIDSDEQVLDWCDRLWLQLQPEDERRLQFWPQHLRELLHEYTLESGRVTMDVLKAMAKLLNQEEGFFIDMVGERFKSYSRFTYYPPCPRPDLVNGLKPHTDNSVITLLLMDKDVGGLQVLKDGNWVDVPVLGHDLLVVVGEGMEIVSNAIFKAPWHRVVTSADKERMSLAMFYQPEPERIIGPPGALVHEKRPPMFKKCLVQTLADGYWDAFAVGDRTVDFLNVRINAEADAELEARAVVANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAQ6</accession>
    <name>A0A8I6YAQ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0410670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0410670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0410670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0342130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0342130.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0410670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0410670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0410670"/>
    </dbReference>
    <dbReference type="OMA" id="AMFYIKE"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Monosaccharide-sensing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
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        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF35">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="612"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
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        <location>
            <begin position="48"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0410670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBZ4</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="125" mass="13624" checksum="C9EAD6BE779BA955" modified="2022-05-25" version="1">MSSVEIPPQGPNAMLGSFTFVDPVQPAQMRDPGEKKRGRCGDLLLADDSEAAGWSSAILDRSLALRRRRGPAAPPSGHRGCLVVTEAQNSRDQEKGPEVVAEEILERQGSEKKPTVEELVYWTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDE8</accession>
    <name>A0A8I6YDE8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDE8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433210.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0521050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521050"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="168" mass="19573" checksum="DCFAA9F635CE9369" modified="2022-05-25" version="1">MGSEFSDAGRRWWIDTYPSAHEVARAYDVVVWHAERPQKHLNFSEIESRVEAKMLVPQGIKMKEITTKKKTTKKPSVVVNAGETDEEAMARFAREHPEYVRAELEHYWKREAEQKKKQDEAGPSTVIPIDSSSEEDCADFSEEEEEEGCDDPEKEEFWEQFRSSDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDN6</accession>
    <name>A0A8I6YDN6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="13">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447906</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. This NDK is microtubule-associated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11 12">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044980556.1">
        <property type="nucleotide sequence ID" value="XM_045124621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDN6"/>
    <dbReference type="SMR" id="A0A8I6YDN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0346530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0346530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0346530"/>
    </dbReference>
    <dbReference type="GeneID" id="123447906"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0289210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0289210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0289210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0346530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0346530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0346530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447906"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="11">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0346530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="16805" checksum="E9F9664C5BBBFAA8" modified="2022-05-25" version="1">MEQTFIMIKPDGVQRGLIGDIISRFEKKGFYLKAMKFMNVERSFAQQHYADLSAQPFFAKLVEYIISGPVVAMVWEGKDVVLTGRRIIGATRPWEAAPGTIRGDHAVEVGRNVIHGSDSVENGKKEIALWFPGGLAEWKSNLHPWIYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFT0</accession>
    <name>A0A8I6YFT0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFT0"/>
    <dbReference type="SMR" id="A0A8I6YFT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0469910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0469910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0469910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0469910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0469910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0469910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF48">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0469910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="170" mass="19420" checksum="E691688C258D2D45" modified="2022-05-25" version="1">MVASGLKLFLPDIISPTQSAFVRGRLITDNVLVAYECFHTIKRKKEGKEDLFAIKLDMHKAYDRVEWPFIKDIMIKLGFHENWVNFIMQCVSTFEYRTRFNTEEIGSFKPTRGLRQGYPMSPYLFILCTEVLTALLTQAEETGGISGVKVCRDAPPVTNLLFGDDSLILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFU9</accession>
    <name>A0A8I6YFU9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123413503</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Cd(2+)(in) + ATP + H2O = Cd(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Zn(2+)(in) + ATP + H2O = Zn(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20621"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962373.1">
        <property type="nucleotide sequence ID" value="XM_045106438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFU9"/>
    <dbReference type="SMR" id="A0A8I6YFU9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727780"/>
    </dbReference>
    <dbReference type="GeneID" id="123413503"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0603650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0603650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0603650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0727780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413503"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008551">
        <property type="term" value="F:P-type cadmium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016463">
        <property type="term" value="F:P-type zinc transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02079">
        <property type="entry name" value="P-type_ATPase_HM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000022">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000043">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051014">
        <property type="entry name" value="Cation_Transport_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085">
        <property type="entry name" value="CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085:SF12">
        <property type="entry name" value="HMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="646"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="13">
        <location>
            <begin position="6"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="747"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="698"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="946" mass="100371" checksum="B894E0D1F7BD5634" modified="2022-05-25" version="1">MAGKLEKSYFDVLGICCPSEVPLVEKLLEPLAGVRKVTVVVPSRTVIVLHDAAAISQAQIVKALNGARLQASVRAYGGGGQSKINKWPSPYVLLCGVLLVASLFQHFWRPLRWLALVATAAGLPPIVLRSVAAARRLTLDVNVLMLIAVAGAVALKDYSEAGFIVFLFTTAEWLETRASCKATAGMSSLMSMAPQNAVLAETGQVVAAQDVKVNTVIAVKAGEVVPIDGVVVEGRSEVDEQTLTGESFPVAKQTDSQVWAGTLNIDGYISVRTTAMADNSAVAKMARLVEEAQNSRSETQRLIDTCAKYYTPAVIVMAAAVAVTPVIVRAHNLRHWFQLALVLLVSACPCALVLSTPVATFCALLMAARTGLLIKGGDVLESLAGIKVAAFDKTGTITSGEFSVAEFRPVGERVPRQQLLSWVSSVESRSSHPMAAALVDYARSNSAKPTPENVMEFQIYPGEGIYGEIDGHGVYVGNRRILSRASCETVPEVNDIKGVTVGYVACNKELVGIFGLSDVCRTGSAEAIRELRSMGIKSVMLTGDSAAAATHAQNQLGNVLAEVHSDLLPEDKVRLVDELKARDGPTLMIGDGMNDAPALARADVGVSMGVSGSAVAMETSHVTLMSNDIRRIPKAVRLARRTRRTIVTNIVFSVATKLAIVGLALAGHPLVWAAVLADVGTCLLVIMYSMMLLRGGDGARHGPRSHIEKHGTSASHHHHCSDGPCKSAGGCGDSSAGGHACGDEHHCHGHGDREEPGSPHHHGCEDHGHGHGHCKEPSKLHPMDSHRCQDHGHGHGHSHCKEASIKLVTSKNISHGHGHSHCKEKHDEHSTNSVESSTQEHSILIDESAAAQQQILCDHQIEEECGHHSKAKARATARPTDCGSLSLRRDTVGDDNEGCGTKARDACSSRRAGCAAGETGRCCRSARASRCGGHASMLKLPEIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHC0</accession>
    <name>A0A8I6YHC0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">indole-3-pyruvate monooxygenase</fullName>
            <ecNumber evidence="3">1.14.13.168</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123406193</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.168" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044955602.1">
        <property type="nucleotide sequence ID" value="XM_045099667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHC0"/>
    <dbReference type="SMR" id="A0A8I6YHC0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556600"/>
    </dbReference>
    <dbReference type="GeneID" id="123406193"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0462330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0462330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0462330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0556600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406193"/>
    <dbReference type="OrthoDB" id="752677at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103075">
        <property type="term" value="F:indole-3-pyruvate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF90">
        <property type="entry name" value="FLAVIN-CONTAINING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="42335" checksum="7E770FC6F2CD88E1" modified="2022-05-25" version="1">MENVTMLIVGAGPAGLATAACLSQFSISHVIVERENCSGSLWRHRAYDRLKLHLGKEFCELLHMSYPADATTYIPKNMFVKYLDDYIEHFNIQPKYLTSVESCIYDNDKKCWSIKALDMAKCMTTDFTTQFLVMATGENSAENIPMVSGLQSFPGETIHSRNYKSGERFYGKKVLVIGSGNSGMEIAYDLASHGANSSIVIRSPIHVMTKELIRLGMTLARHLPLHLVDNLLVMGANYIFGDLSRYGITRPKLGPMSLKSRTGRSAVIDVGTVGLIKKGIIKVQREVTNIKGRTVEFQCGNKVSFDAIVFATGYKSTTNIWIKNNESMLNENGMPIKEYPKHWKGENGLYCTGLARTGLAGIAMDVKNIANDINDMIGSFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHN8</accession>
    <name>A0A8I6YHN8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123408471</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044957509.1">
        <property type="nucleotide sequence ID" value="XM_045101574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHN8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637150"/>
    </dbReference>
    <dbReference type="GeneID" id="123408471"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529470.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0637150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408471"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF1">
        <property type="entry name" value="OS06G0109100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035218691" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="30"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="104"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="170" mass="18572" checksum="C45DDD7984971166" modified="2022-05-25" version="1">MGFPAVYSEMPRLLLNLLFLLGHLRRLSSWLLRLVGAGVDDDLSFDYSDASDAAYFADHHHHQHQHDHGHGLEELEEHSPAVRFDALSAASAGDDGTPLLPEGCAVCLGDFHGAARVRRPRGCRHVFHRGCLDRWAAHGHRTCPLCRAPLLPPPATAPVLLPLPVPLPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YI08</accession>
    <name>A0A8I6YI08_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YI08"/>
    <dbReference type="SMR" id="A0A8I6YI08"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0727700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="228" mass="24926" checksum="B3622095CEC9B643" modified="2022-05-25" version="1">MPHLYDLYGMAHTASYKTAKAFSESDLDDPNNFTNISSHHKLVAYRDTGKATKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKVRQSSSCPEITRRLRPVDLAIEAALQKERLANQAALEKERLANQAAIEKERLANQAALQAALDERDQTTTRLIEEERSRNEAGQRALYKLFVGLCEKSGQVPPPMPVFSSIGTNNSRAASHDPSRGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YI61</accession>
    <name>A0A8I6YI61_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <evidence type="ECO:0000313" key="2">
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        </source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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    <sequence length="493" mass="54070" checksum="6B79F94C08922D40" modified="2022-05-25" version="1">MEGSSGPRRSSTQDVGSPAGGADLISALPDEVILLFLARLPCASAAARTGVLSRRWRGLWSLLRQIVFRDVPFPSLEAALGRVLPPPHAVSLLEICVPMEQSTDSAGFNSLLRAAARLEPEKLDFRLPSHLTDLPSGSRIAVDLPCSHRATSISLGLSTPFSLRMPAGAQFPGIEALSLSDCTTELDALLSCCPRLRTLSVTRGLYPNYKCDIRVNSPSLQELAVHHRESETQHVDIVAPALKQLALSFSNGGEISISVLAPMVEKVSWHCCYLGHRIVSGLWSLRKLKLRLQTAERQGQLSSLQIHARADLSFLHAQGDNFTQEIGKHIMVAPFSCLELHLTAKGHAYGGFVFHLLRVDRIHAATRRLKLILNRSAMEGGCPLHCPCEFRNWRFETISLPALEEMEFNGFQGEDHEFDLLQLILGCAPTLKRMVVHLSKETSASHEGCAKIYSIFKACSSVKCDVYHSSGLMHGSQNCPSMRFSGVLIYGAH</sequence>
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    <accession>A0A8I6YKI5</accession>
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                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
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    </evidence>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953482.1">
        <property type="nucleotide sequence ID" value="XM_045097547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKJ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600630"/>
    </dbReference>
    <dbReference type="GeneID" id="123403626"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0497910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0497910.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0497910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0600630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0600630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0600630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403626"/>
    <dbReference type="OrthoDB" id="1080706at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34059">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34059:SF3">
        <property type="entry name" value="OS02G0656100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0600630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="301" mass="31870" checksum="3EB88F3910E4B1FB" modified="2022-05-25" version="1">MAGSAAQAPTQTGRRTPIPTALVPAAVLLAVVVAVLSLLPSVAQAVWELPHLFLLGLVISYGVFAQQKNGAASAGDGDATKDGARAWNSRYHPDDPLVVVAPGHAEGDDGDDGAGGRPFSLPVRRLRTVVEEPTEAGGASGESVGEETDSSEYTAGFWAGAPAAPSPPSVLDSFDSRKFNAATPPPTMSKGFPGYDSASPRDQSSCNEEEEAEEVEEEEEEGTDWDEDADGSDEMTAASSERSFPGDFVACRNRRYDGSGDGESVDEELIELATRPGPDEVDRKADEFIAKFREQIRLQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YM25</accession>
    <name>A0A8I6YM25_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YM25"/>
    <dbReference type="SMR" id="A0A8I6YM25"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0438480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0438480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0438480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0527200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF61">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="66"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="233" mass="25651" checksum="95BE68915BDEBE7E" modified="2022-05-25" version="1">MAPSVQVSAVAAPHVLLLSRRLAYAPVSAPSVPLLRSGSGRLTRRASVRVRAGAGGGSGGGPRREAPHDVLGVAPSASPAEIKRAYRRLALKYHPDVNKEANSQEKFLRIKHAYNTLMSSESWSKYASSSSKPADAEEEFDWLAAIIKEMETYRKAEDGLDLEWKIESVAEVVFTYCLTIAMLIFVYQKELIVLIKSLSLEVTFNCFKPHDPSSLTKREAIDDQFNATALNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVB2</accession>
    <name>A0A8I6YVB2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123410125</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044958947.1">
        <property type="nucleotide sequence ID" value="XM_045103012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVB2"/>
    <dbReference type="SMR" id="A0A8I6YVB2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0686030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0686030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0686030"/>
    </dbReference>
    <dbReference type="GeneID" id="123410125"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0569500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0569500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0569500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0686030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0686030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0686030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410125"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000067">
        <property type="entry name" value="calcium-dependent protein kinase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000050">
        <property type="entry name" value="Calcium-dependent protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="85"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="414"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="517"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="36"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0686030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="579" mass="63845" checksum="FCB2A98E80270199" modified="2022-05-25" version="1">MGGCYSAYACSRKLRGRLGNLSFVLPVTERDAAAANAAAGTSSTSSAKKGDHGSSRRSNGEGPAGGTEEEELVTKTTTAEFGRRYVLGKELGRGEFGVTRRCRDAATGEALACKTIRRHRRRGGRGLNRKPAGGGGAEAAARAAAAAAAAHAADVRREVAIMRRMSARGGAAVVRLREAREDQDGSVHLVMELCEGGELFDRIVARGHYSERAAAKIFRTIVNVIQICHSNGVIHRDLKPENFLFANKSEDAALKVIDFGLSVFFNPGDRFTEVVGSAYYMAPEVLKRNYGQEVDVWSAGVILYILLCGVPPFWGDNDEKIAQAILRGGLDFNREPWPRVSGNAKDLIRRMLDPDPATRLTAHQVLEHPWLKNADTAPNVSLGEAVRSRLQQFSAMNKLKKKALGVVARNMPVEELDKYVQMFHLMDKDKNGNLSLEELMEGLHINGQRVPESEIRMLLEAADTDGNGTLDCDEFVTVSLHLKKMTNDKYLAAAFRYFDKDGSGFIEIDELRQELGPNEQAILEIIRDVDTDRDGRISYQEFELMMKSGADWRNASRQFSRANFNTLSRKLCKQENSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXI9</accession>
    <name>A0A8I6YXI9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protease Do-like PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401764</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951552.1">
        <property type="nucleotide sequence ID" value="XM_045095617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXI9"/>
    <dbReference type="SMR" id="A0A8I6YXI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0615620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0615620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0615620"/>
    </dbReference>
    <dbReference type="GeneID" id="123401764"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0510450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0510450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0510450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0615620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0615620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0615620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401764"/>
    <dbReference type="OrthoDB" id="4217619at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000012">
        <property type="entry name" value="protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000131">
        <property type="entry name" value="Protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF18">
        <property type="entry name" value="PROTEASE DO-LIKE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="308"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Protease Do-like PDZ" evidence="7">
        <location>
            <begin position="417"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0615620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="61112" checksum="391232410A465FE0" modified="2022-05-25" version="1">MDGSASKGKRKRGRKSKAAADHRSPSPDAPAAAAADNPAPAAAGRRGRKPRRVEVEAPAAADASTPRRGDAKHLANGGDAAMAELGPAGWEEVVRVVPSMDAVVKVFCVHTEPNFSLPWQRKRQYSSSSSGFIIGGRRVLTNAHSVEHFTQVKLKKRGSDTKYLATVLAIGTECDIALLTVSDDEFWEGVTPVQFGSLPALQDAVTVVGYPIGGDTISVTSGVVSRIEILSYVHGSTELLGLQIDAAINSGNSGGPAFNDRGICVGIAFQSLKHEDVENIGYVIPTPVIKHFIQDYEKSGAYTGFPIIGIEWQKMENPDLRKAMGMKPDQKGVRVRRVEPTAPESGCLQPSDIILSFDGVDIANDGTVPFRHGERIGFSYLVSQKYTGDKALIKVLRDSKVHEFKIKLATHKRLIAAHVKGRPPSYYIVAGFVFAAVSVPYLRSEYGKDYEYDAPVKLLVKHLHSMVESPDEQLVVVSQVLVADINIGYEEIVNTQVLAVNGHPVKNLKDLVTTVENCKDEFLKFDLEYDQIVVLETKTAKAATQDILTTHCIPSAMSDDLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z496</accession>
    <name>A0A8I6Z496_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123401208</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044950878.1">
        <property type="nucleotide sequence ID" value="XM_045094943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z496"/>
    <dbReference type="SMR" id="A0A8I6Z496"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623070"/>
    </dbReference>
    <dbReference type="GeneID" id="123401208"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0517020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0517020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0517020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0623070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401208"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="321"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="492" mass="52550" checksum="EE0477419EC65364" modified="2022-05-25" version="1">MGFLSLVGNSFGCSASGERLVSAARDGDLQEARALLEYNPRLARYSTFGGRNSPLHYAAAQGHHEIVSLLLESGVEINLRNYRGQTALMQACQYGHWEVVQTLMLFNANIHRTDYLNGGTAIHFAALHGHARCLRLVLADYVPSIPNFLNQTKHRSSEEVSDADFDDDGLVKMVNWKADGGLTPLHMAALNGHVECVQLLLDLGASVSEVTIEDGTTIDLLGSGSTPLHYAACGGNAVCCQLLIARGANLAAKNASGSTPLMVAHSWHRNSIEEILSNEPGGQIRTLPSPYLCLPLMSIMSIARECGWRYLNKSPVCIDPCAVCLDGSCSVAAEGCKHEFCTRCALYLCSTSYTSANPAGAIPCPLCRHPIISFVALPGTSPKRELPRNSLSLSFCATCPAVSSDSAAPGGTEFHCGSGVRMPPMGSSSFRSLSCQAMKLNPSFCMGAMDTNPCLIRCSRFGSSLNRSASHGETNRRGAWPVTFSPIVATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z775</accession>
    <name>A0A8I6Z775_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123410638</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959517.1">
        <property type="nucleotide sequence ID" value="XM_045103582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z775"/>
    <dbReference type="SMR" id="A0A8I6Z775"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653390"/>
    </dbReference>
    <dbReference type="GeneID" id="123410638"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542090.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410638"/>
    <dbReference type="OrthoDB" id="645938at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF237">
        <property type="entry name" value="OS06G0156700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035277877" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="40600" checksum="7294A949A61DACEF" modified="2022-05-25" version="1">MARRINGVTAAASASLLLAFLMPAAADGDGGGTTRYNAMFNFGDSLSDTGNLCVNKSAAAELLLTFANPPYGMTYFGRPTCRCSDGRLVVDFLAQSLGLPFLPPSKLPGADFRKGANMAIIGGTALDFEFLKSIGLGYPIWNNGAINVQLRWFRDLLPRVCGGPQTCRPYLARSLFLLGSIGGNDYNAMLFFGFNVSRAKSYAPNVVDNIAAAVERLIELGAADIVVPGTLPIGCIAVYLAILPSGDKSDYDERGCLKPLNGLAMYHNSLLQDRLNGVRARHGSAVRIMYADYYAHAVDMLRDPARFGFTTVITACCGAGGGPYNYEFDARCGMKGATACADPWRHESWDGVHPTEAVNKLVADGWLRGPYCHPPILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z907</accession>
    <name>A0A8I6Z907_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956722.1">
        <property type="nucleotide sequence ID" value="XM_045100787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z907"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747260"/>
    </dbReference>
    <dbReference type="GeneID" id="123407621"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0619890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0619890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0619890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747260"/>
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    <dbReference type="KEGG" id="hvg:123407621"/>
    <dbReference type="OMA" id="MDAQMQE"/>
    <dbReference type="OrthoDB" id="653441at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37194">
        <property type="entry name" value="T2E6.7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37194:SF2">
        <property type="entry name" value="T2E6.7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="89" mass="9701" checksum="5D7242026F55D137" modified="2022-05-25" version="1">MEMNTATSLEPRAKATLVLGGEQFAVSSESGTLSEQLAVMREKSMVILKEYITKHNVPNDVPDESIEGPSDEEGEALAKNPPKKSKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z916</accession>
    <name>A0A8I6Z916_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123411228</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960101.1">
        <property type="nucleotide sequence ID" value="XM_045104166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z916"/>
    <dbReference type="SMR" id="A0A8I6Z916"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0671320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0671320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0671320"/>
    </dbReference>
    <dbReference type="GeneID" id="123411228"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0557100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0557100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0557100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0671320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0671320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0671320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411228"/>
    <dbReference type="OMA" id="PVHERQY"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="12 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="76"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="181"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="281"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0671320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="378" mass="41339" checksum="AA72E383F5DEDBE8" modified="2022-05-25" version="1">MDELRPPSVVQKLHGQSMLLRQISSFAMRSPVIDNAHFARNFSVTKYHQTNGSIGRLPLNTPSPMRAPAPEEKGFSGFMIDFLMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKSGRLSHPYKSIGDCFSRTIKDEGVVALWRGNTANVIRYFPTQALNFAFKDHFKRMFNFRKDKDGYWKWFFGNIASGGAAGASSLLFVYSLDYARTRLANDAKAAKVGGQRQFDGIVDVYRKTLASDGVRGLYRGFNVSCVGIIVYRGLYFGMYDSLKPAVLVGSLEDNLLASFLLGWGVTMGAGLASYPIDTVRRRMMMTSGESVKYKNSMDAFKKIVAEEGAKSLFKGAGANILRAVAGAGVLAVYDKLQVVVFGKKYGSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0P8</accession>
    <name>A0A8I7B0P8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0P8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0023080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0023080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0023080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0029190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004252">
        <property type="entry name" value="Probable_transposase_24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03004">
        <property type="entry name" value="Transposase_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="17174" checksum="0942814211356472" modified="2022-05-25" version="1">MRQADPEKKRPHRSQVHLATHKKKDEANAENIDRNESLDRLKNLITERPELAKKLNGRVAWEGDALQEVFGKEKIGQVHGISLLPTPKQVYGWTPRYLKNINMTTTDGSPYEVEHDVWEEIAKMKEHIKRQYQIIEHMKNKEGNVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6P8</accession>
    <name>A0A8I7B6P8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447219</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cytoprotective ribonuclease (RNase) required for resistance to abiotic stresses, acting as a positive regulator of mRNA decapping during stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044979737.1">
        <property type="nucleotide sequence ID" value="XM_045123802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6P8"/>
    <dbReference type="SMR" id="A0A8I7B6P8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335150"/>
    </dbReference>
    <dbReference type="GeneID" id="123447219"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0279480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0279480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0279480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447219"/>
    <dbReference type="OrthoDB" id="10023235at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:mRNA catabolic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20443">
        <property type="entry name" value="Tudor_AtTudor1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000011">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Silence_cplx_Nase-comp_TudorSN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047395">
        <property type="entry name" value="Tudor_AtTudor1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="14">
        <location>
            <begin position="183"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="14">
        <location>
            <begin position="378"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="14">
        <location>
            <begin position="588"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="13">
        <location>
            <begin position="780"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="989" mass="108625" checksum="C1AA2DCB19EC1AC3" modified="2022-05-25" version="1">MASNTGASGWLRGKVKAVTSGDCLLIMGSTKAEIPPEKSITLSYLMAPRLARRGGVDEPFAWESREFLRKLCVGKEVTFRVDYTAPNIGREFGTVYLGDKNVAYLVVAAGWARVKEQGPKGGEPLPNVSELIRLEEVAKQQGLGRWSKEPGAAEESIRDLPPSAIGELSGFDAKGFAVANKGKSLEAIVEQVRDGSTVRVYLLPSFQFVQIYVAGVQAPSMGRRPPNPTVVAEPEGTTNGTTNGDDSGEAPAPLTTAQRLAASTASTEVPPDRFGREAKHFTETRVLNRDVRIVVEGTDSFSNIIGSVYYPDGDTAKDLSLELVENGLAKYVEWSANMLDVEVKIKLKNAELQAKKEQLRIWTGFKPPVTNSKPIHDQKFTGKVVEVVSGDCIIVADDAAPYGSPSAERRVNLSSIRAPKLGNPRREDNKPENFARESKEFLRTRLIGKQVTVEMEYSRRISTMDGQNAMPAVNSADTRVLDYGSVFLGSPSQADGDDTSSVPSTGTQPKINVAELLLSRGFAAISKHRDYEERSHYFDALLAAHSRAEKAKKGVHSDKLPPVMHITDLTMVNSKKAKDFLPFLQRNRRHTAIVEYVFSGHRFKLTIPKETCSIAFSLSGVRCPGKDEPYSSEAIALMRRMVLQRDVEIEVETVDRTGTFIGSLWESKTNVGSVLLESGLAKLSSFGLDRIPDAHVLTRAEKLAKQQKLKIWENYVEGEEVTNGSASESKQKEILKVVATEVLGGGKFYAQTVGDQRVASIQQQLASLKLKEAPVIGAFNPAKGEIVLAQFSVDNSWNRAMIVNGPRGAVESVDDKFEVFYIDFGNQEVVPYNRIRPADPSVSSSPPLAQLCSLAFIKVPGLEDDNGQEAAEYLSECLLSSSKQYRAMIEERDTTGGKVKGQGTGPVLIVTLVDPETESSINAAMLEEGLARLERGKRWDTKERKTALENLEQFQEKAKKERLRLWQYGDVESDEEEQAPGGRRPPPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8R5</accession>
    <name>A0A8I7B8R5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0452510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="22467" checksum="62AE4D478B030AB7" modified="2022-05-25" version="1">MEFFTIILEKSSSRQVLSDNFVKMLDGHRPKNMKLRQTGNGLRKLWDVEVAFDTDGSMHLDRCWKQFLCAYDLRHGYFLIFRYDDNATFAVKVFDTTMCRRRYQDDDDASNGSRSSEYCDMCYVYGSSDSDYSKSSSDSGYSKSSSDDGLAMVIPQLGDRAMPILVEEYIPQPGLGLRRSKRIRMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BF97</accession>
    <name>A0A8I7BF97_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">MYB transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123398226</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Forms a homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044948657.1">
        <property type="nucleotide sequence ID" value="XM_045092722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BF97"/>
    <dbReference type="SMR" id="A0A8I7BF97"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0508400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0508400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0508400"/>
    </dbReference>
    <dbReference type="GeneID" id="123398226"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0422210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0422210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0422210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0508400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0508400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0508400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398226"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Telomere repeat-binding factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044597">
        <property type="entry name" value="SMH1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267">
        <property type="entry name" value="SINGLE MYB HISTONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267:SF8">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0779">Telomere</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="14">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="15">
        <location>
            <begin position="122"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0508400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32737" checksum="047334A126FF7708" modified="2022-05-25" version="1">MGARKQKWTSREEAALRAGIAKHGVGSWRVILTDQEFSSILSCRSNVDLKDKWRNMNVFSTESGSMNKGRTATKKNRATPKRNDHSMAISTVASDVDDEIVDEQPIASVSSEVWNVLIPKKSRSRLNNIILESVKNLNEPTGSHRTTIANYIEEEYLPPGDFDHILSANLKDLTTSGELIEVNRNYRIAPAPGSMYSEGRSPETLLLEDIQGEPQMTESDDIETLTKSRVDAELAHMITMTAKEAASAAARAVAEAEALMAEAEAATREAEAAEADAQAAQAFAEAVRSRNVTELMAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFK9</accession>
    <name>A0A8I7BFK9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GH10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFK9"/>
    <dbReference type="SMR" id="A0A8I7BFK9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003305">
        <property type="entry name" value="CenC_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031158">
        <property type="entry name" value="GH10_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF89">
        <property type="entry name" value="GH10 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02018">
        <property type="entry name" value="CBM_4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00591">
        <property type="entry name" value="GH10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBM-cenC" evidence="10">
        <location>
            <begin position="13"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="9">
        <location>
            <begin position="323"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="219"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="55078" checksum="A36857DD177478D6" modified="2022-05-25" version="1">MAGTQDASLITQNSTLEGGSLAGFAALGSRTTTLSVHDEEEMVAVTVGHGGDGNEPSGRYILVSGRADEKDGLRQVITGGVLKPGITYRVAGWISLVVAGAASRGTSHPVRVNLGVAMDDDESESLQVECGAVCAEAGGWTEIMGAFRLMTEQRSAAVYVHGAPAGVDVKVMDLRVVSVDREARFRQLKDKTDKHLIVLALLHQPLGLDRLRERAQVVPHRGAAGGSSTTPTPTRSSRSATAWTSASAATASSGPWKATCSSGSRAWTRTSSSLPSRATSRAWCPATLATSRITTSTTRCCTANSSGTVSETRTSRRSCSRRYANDFNVECGNDPNATPEKYAEQVAWLQSCGAVVRGIGLQGHVTSPVGEVICAALHRLATTGVPVWFTELDVSEPDVNLRAKDLEVVLREAYAHPAVEGIVLWGFMQGTMWLQDAWLVDANGTVNEAGQMFLNLQREWKTDVRGNADGDGNFNFRVFHGRYFVEVTTATGCRMLKTFTVEKGDNTPLLVDLGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BG59</accession>
    <name>A0A8I7BG59_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123406272</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955693.1">
        <property type="nucleotide sequence ID" value="XM_045099758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BG59"/>
    <dbReference type="SMR" id="A0A8I7BG59"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540940"/>
    </dbReference>
    <dbReference type="GeneID" id="123406272"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0449880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0449880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0449880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406272"/>
    <dbReference type="OMA" id="ATWSCFL"/>
    <dbReference type="OrthoDB" id="773329at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF55">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035163563" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="21"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="883"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="38"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="185"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="943" mass="104559" checksum="3706E6AAC796784C" modified="2022-05-25" version="1">MDRSLANFVLLLMAAASTWSFFLVAADAGRLQPSCIGREREALLAFKQVINDTDDSLGSWQQERHDCCQWAGITCSNTTGHVIKLDLGYANLVGQISPSLLSLEHLEYLNLRNNGLFGSDGRVPEFLGSLKNLRHLDLSEMSFSGTVPPQLGNLSKLEYLNLDLSDIRYFNNTGMVSTDISWLARLPLLVHLGMSGMNLSSIADLPVTVNKLPSLEFLGLSECLLSSANQSLTHLNLTNLQDLDLSHNYFGHPIASGWFWNLTSIEYLDLSDTSLHGPFPNALGNMTSLQWLSLSSMGNRATMTVDLKSLCDLENLWLDGSLSSGNITEFLDKLPRCSSNKLQRLNLSSNYMVGILPHRMGHLTNLSWLDLSYNNITGAIPLGIGNLSCLENLFISNNLLTGAIPPGLGNSTFVYLSNNNLSGPIQLGIESCTRLQYLDLSYNSITGPIPSMLGNCTKLYYLALSNNLLTGDIPPGLGNCTSLQYLSLSKNLLTGHLTSEISLLGNLTELDLSNNNLDGVITGEHLVTLKNLEHLDLSHNSFSGPLPSEFGAYRLVELTLSYNYFSGHIPESVCMLRNLLVLDLSNNLLVGEFPRCSQKPILVFLLLSHNRFSGKFPSSLKNYSSLAFMDLSTNSFHGTLPLWIGDLVYLRFLQLSHNFFSGHIPITITNLKRLRQLSLAGNSIFGILPWSLSNLTAMTQKHTKRPGIDMSIWYTGYVGKFREVWPIVMKRQELKYGTGIFYVVSMDLSLNYLTGEIPDGLTSLNGLLNLNFSWNQLSGKIPGKIGAMKSLESLDLSRNNLSGEIPTSLSDLTYLSSLDLSYNNLTGRIPPGRQLDTLYLDNQSIYRGNFGLCGTPLERNCSGDNALEGDNQQKIEKASEHVLFFYSGLGSGFMAGLWVVFCTLLFKKVWRVAYFRLFDKLYDKVYVFLVVTWGRINHKETTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BG61</accession>
    <name>A0A8I7BG61_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123403419</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953290.1">
        <property type="nucleotide sequence ID" value="XM_045097355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BG61"/>
    <dbReference type="SMR" id="A0A8I7BG61"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541150"/>
    </dbReference>
    <dbReference type="GeneID" id="123403419"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0450060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0450060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0450060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0541150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403419"/>
    <dbReference type="OMA" id="ITFEYED"/>
    <dbReference type="OrthoDB" id="621599at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF95">
        <property type="entry name" value="OS11G0564900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035248698" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="32"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="833"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="40"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="893" mass="99425" checksum="6C0B195283ACEB56" modified="2022-05-25" version="1">MDSSPSAKSVLLLMAAAAAWISFFLVADASAGAVACIRRERDALLAFKQGINDTDDELRSWQRGSQDCCRWAGITCSNMTGRVIGLDLSRRFSLVGQISPSLLSLEHFQYLNLKSTSLCGHGGRIPEFLGSLNNLRHLDLSYMSFSGVLPPQLGNLSKLEYLDLSNMEMDVIDISWLSRLPRLMYLDISYTNLSSIAAWPPVVNMIPSLKDLRLSYCSLSSTNQSLTHLNLTNLQHLDLSRNYFAHPIASSWFWNVTSIEYLDLSDTSLHGPFPNALGKMTFLRQLSFFGIGNTATMTVDLKNLCDLEIIWLDGSLSSGNVTEFLKKLPRRCPSNRLQELKLSSNNMVGMLPNRMDYLTNLSSLDLSYNNITGAIPPWLENCTSLSYLSLSSNSLTGPIPVGIGRCTLLDILDLSYNNITGAIPLGIGNFTTLRYLVLSHNLLTGHVPSKIGMLGDLIDLDLSNNNLDGLFTREHMVSLKNLRHMDLSHNSFSGPLPIETRAQFLKELTLSSNYFSGHIPESICQLRSLLVLDLSDNFLEGELPHCSHKPNLVFLLLSNNGFSGKFPSSLRNYSSLAFMDLSWHNLYGTLPFWIEELVNLRFLQLSHNLLYGDIPVTITNLQHLHQLSLAGNNISGAIPESLSNLTSMAQKDPQNSEDYMSAWYNNNVGTFRQVWHVVMKRQELKYGAGIFDVVGIDLSLNHLIGEIPEMITSLGGLLNLNLSWNHLSGKIPGKIGAMKSVESLDLSRNNLYGEIPASLSELTFLSSLDLSYNNLTGIIPRGSQLDTIYIENPAIYTGNIGLCGPPLERNCSGNNSLEHVNQPRRDNVYEAKMFFYFGLGSGYVAGLWVVFCAMLFRKAWRVAYFRLFDKLYDKAYVFAVLTWGRINGKASTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIT3</accession>
    <name>A0A8I7BIT3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIT3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0673280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0673280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0673280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0558740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0558740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0558740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0673280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0673280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0673280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF45">
        <property type="entry name" value="OS06G0294000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="169"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="383"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0673280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="491" mass="53243" checksum="C1C661848DAC5376" modified="2022-05-25" version="1">MGPDGKKPSGPSSTPSVPSSTEYQLKKYLLLLATLVATVTYAAGLNLPGGVWQDTKDGHLVGDLILHDTHYHRYLTFYYCNATAFAASVVVCLLLLILDSESKPWAAALRVLMVLDLLGLMGAYAAGSCRDAFTTIYSSVIMSAVLAYIAIAFFVYVFSKKQGEDSEKHKREDQHEVLMLLATFVVTITYVAGLNPPGGFWSNSQDGHRVSNPVLQEHNSSRYQAFFVCNTTAFVSSLLIIILLLDKKLILSLPANKLSGPFVRFLALYVFIIIALFGLVGAYAAGSCRELDTTTYVVCLIGVVLGYIFLQVAITKAIPENATGWLKNIKELFVESSRGNEALGKDEASEAMERAKTNEASKRTNKAPVKANAKESRNSQANEALEKARNLVILLATLVASITYQAGLDPPGGLWPDDQDGHKGGDPILLTTHPTRYKVFFYSNSAAFVASLIVITMVQSSSLLKRHTLEAAMLLDLFGLIGAYAAGSAEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIV8</accession>
    <name>A0A8I7BIV8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIV8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0676510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676510.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28938" checksum="222D10DD747C395D" modified="2022-05-25" version="1">MPGRPSRQVVRLTKCINVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGVEKPIHWSYGLGVTVDMAVLDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEILENRDKDARALRMELYATRARLWTALTQLAPVVQKGYGEMEMLNPVRNHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-01-23" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A1C0L4</accession>
    <name>A1C0L4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hexosyltransferase</fullName>
            <ecNumber evidence="15">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">UCW98</name>
        <name evidence="17" type="synonym">LOC123407177</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="Proc. Natl. Acad. Sci. U.S.A." volume="103" first="19581" last="19586">
            <title>The wheat and barley vernalization gene VRN3 is an orthologue of FT.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Fu D."/>
                <person name="Li C."/>
                <person name="Blechl A."/>
                <person name="Tranquilli G."/>
                <person name="Bonafede M."/>
                <person name="Sanchez A."/>
                <person name="Valarik M."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="17158798"/>
            <dbReference type="DOI" id="10.1073/pnas.0607142103"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Golgi apparatus membrane</location>
            <topology evidence="2 15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="15"/>
    <dbReference type="EMBL" id="DQ900685">
        <property type="protein sequence ID" value="ABL11234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A1C0L4"/>
    <dbReference type="SMR" id="A1C0L4"/>
    <dbReference type="CAZy" id="GT31">
        <property type="family name" value="Glycosyltransferase Family 31"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654060"/>
    </dbReference>
    <dbReference type="OrthoDB" id="414175at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000012">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF290">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABL11234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="365" mass="40287" checksum="B77EB78B4F6BF0AB" modified="2007-01-23" version="1">MPSSPKVFSSSAAAASRRSSLRRLLSSPAVSAACLLFGLTGFLAAAVSLSRGPAEAPRGRCPDSSHPLSVSVAWDRRPGDAAGGATEFPAGHATGSRGRHKVMAFVGIFTGFGSVGRRRALRRTWLPSDRQGLLRLEEATGLAFRFVIGKSNDKSKMTALEREVEEYDDFVLLDLEEEYSRLPYKTLAFFKAAYALFDSDFYVKADDDIYLRPDRLSLLLAKERPHPQTYIGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYALSADVVASLVALRNNSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTASSIAVWDIPKCSGLCHPEVKMLELHRRKECTGGPTEAAETETDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2CS07</accession>
    <name>F2CS07_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123426754</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK354409">
        <property type="protein sequence ID" value="BAJ85628.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966571.1">
        <property type="nucleotide sequence ID" value="XM_045110636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CS07"/>
    <dbReference type="SMR" id="F2CS07"/>
    <dbReference type="FunCoup" id="F2CS07">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2CS07"/>
    <dbReference type="PaxDb" id="4513-MLOC_12172.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027090"/>
    </dbReference>
    <dbReference type="GeneID" id="123426754"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0021410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0021410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0021410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426754"/>
    <dbReference type="eggNOG" id="ENOG502QSP6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_12_1_1"/>
    <dbReference type="OMA" id="MGIKYVR"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CS07">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd07245">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46142:SF3">
        <property type="entry name" value="F18B13.24 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="22"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ85628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="23234" checksum="91056978C1164B2B" modified="2011-05-31" version="1">MLNTTVAMVSSGGKGSVLPLASLNHISIVCRSVEESLDFYMNVLGFTPIRRPGSFDFDGAWLFNYGIGIHLLQSEHPESLPAKKEINPKDNHISFQCESMVAVERRLKELGIQYIKRCVEEGGINVDQIFFHDPDGFMIEICNCDNLPVVPLADQTFAMAACKRFAPVKQQQLPMPVAQAPPAPPAAATQCIPAPNPAMQRVGAEDVAHISCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXM2</accession>
    <name>F2CXM2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uncharacterized protein ycf23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123450485</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot</tissue>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ycf23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK356375">
        <property type="protein sequence ID" value="BAJ87593.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK363273">
        <property type="protein sequence ID" value="BAJ94477.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983642.1">
        <property type="nucleotide sequence ID" value="XM_045127707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXM2"/>
    <dbReference type="SMR" id="F2CXM2"/>
    <dbReference type="PaxDb" id="4513-MLOC_4845.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088680"/>
    </dbReference>
    <dbReference type="GeneID" id="123450485"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0072930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0072930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0072930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0088680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0088680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0088680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450485"/>
    <dbReference type="eggNOG" id="ENOG502QUDA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5415at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CXM2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007570">
        <property type="entry name" value="Uncharacterised_Ycf23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36895">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36895:SF1">
        <property type="entry name" value="YCF23 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04481">
        <property type="entry name" value="DUF561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ87593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ94477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0088680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="298" mass="30732" checksum="A885C1A6B16B5A4C" modified="2011-05-31" version="1">MAAPGSLALLQAPSFAAAKCRPVAMSRGAARAVFAVRASAASAATKDAVLRPFRENRALKIISGLQNFDRSNVASVVTAADKGGATHVDIACDQDLVKLALELTNLPICVSSVDPSAFQSAVEAGAQMIEIGNYDSFYDAGIEFSSEQILNLTRETRKILPGITLSVTVPHTLSLPDQMRLAELLEEEGADIIQTEGGKSSSPTKPGVLGLIEKATPTLAAAYSISRAVSIPVMCASGLSSVTAPMAVTAGAAGVGVGSAINKLNDVVAMIAEVRSIAEALGTTASRNVSEDLRTIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZ66</accession>
    <name>F2CZ66_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123399475</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356922">
        <property type="protein sequence ID" value="BAJ88137.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949822.1">
        <property type="nucleotide sequence ID" value="XM_045093887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZ66"/>
    <dbReference type="SMR" id="F2CZ66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491320"/>
    </dbReference>
    <dbReference type="GeneID" id="123399475"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0491320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000382">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF702">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730780" evidence="2">
        <location>
            <begin position="31"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ88137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="337" mass="37465" checksum="D9D3C4759869CCD5" modified="2011-05-31" version="1">MAPPRRVCVTGGGGFIASWLVKLLLSRGYAVNATVRDPSDPKNVHLKQMDEARENLHLFRADVLDYDTLTRAFEGCEGVFHLATPVPEDKIVDPESEVLAPAVKGTSNVLKACSAMKVQKVIVLSSTAAVDFNPNWPQDKLKDESCWSDKDFCQKNEDWYSVAKIVAEQASFEYSEKNGLNVVTVCPPLVFGPLLQPTVNTSSKFLIYVTNGGPDVMSNKLWHIVDVRDVADALLLVYEKAESYGRYICTPNNICTTDLVDLLKKMHPQYSYVNNIVDVDRKAPISSQKLKDLGWKPRKLEETLSDSVDCYEKAGLLQGSAARPCRLPHLFRLAGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F2DF63</accession>
    <name>F2DF63_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123410028</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK362530">
        <property type="protein sequence ID" value="BAJ93734.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK374734">
        <property type="protein sequence ID" value="BAK05930.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958835.1">
        <property type="nucleotide sequence ID" value="XM_045102900.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958836.1">
        <property type="nucleotide sequence ID" value="XM_045102901.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958837.1">
        <property type="nucleotide sequence ID" value="XM_045102902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DF63"/>
    <dbReference type="SMR" id="F2DF63"/>
    <dbReference type="PaxDb" id="4513-MLOC_34756.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708730"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708730.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708730.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708730"/>
    </dbReference>
    <dbReference type="GeneID" id="123410028"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708730.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708730.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410028"/>
    <dbReference type="eggNOG" id="KOG1562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_3_0_1"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DF63">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000003">
        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000048">
        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF29">
        <property type="entry name" value="AMINOPROPYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="52"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ93734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="385" mass="42090" checksum="7E050387FFB1C58E" modified="2011-05-31" version="1">MEGGGARNVSAAAVQTKGTGDDGSRKPLPPCCVKAQAAVAESEAKCHATVVSGWFTGTRSRSGKPSKAQYFNNPMWPGEAHSLKVEKILYQGKSPYQEVLVFESSTYGNVLVLDGIVQLTDKDECAYQEMVTHLALCSIPSPKNVLVVGGGDGGVLREIAKHDSVESIDICEIDQLVIDVCKDFFPRLYVGYKDPRVRLHVGDAVEFLRNSPEGKYDAIIVDSSDPIGPAQELVEKPFFQAIARALKPGGVLSNLAESMWLHTHLIQDMLSICREVFKGGVHYAWASVPTYPSGVIGFLLCAKEGPPVDFLTPVNPIEKIEGATKDGREMRFYNSEIHRAAFILPTFVKRELEAYNTSTEKEKLEKPTAKPVKMKVMRDSAITAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2DHG3</accession>
    <name>F2DHG3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123411187</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK363330">
        <property type="protein sequence ID" value="BAJ94534.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960061.1">
        <property type="nucleotide sequence ID" value="XM_045104126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DHG3"/>
    <dbReference type="SMR" id="F2DHG3"/>
    <dbReference type="PaxDb" id="4513-MLOC_20326.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0663690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0663690"/>
    </dbReference>
    <dbReference type="GeneID" id="123411187"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0550580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0550580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0550580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0663690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0663690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0663690"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPP6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FCDDEPE"/>
    <dbReference type="OrthoDB" id="1927218at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DHG3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009789">
        <property type="term" value="P:positive regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000048">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF446">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-LIKE PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="250"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ94534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="332" mass="36056" checksum="0A06A2635FC5DADC" modified="2011-05-31" version="1">MEMPGGSGGGALARQGSIYSLTFDEFQSALGGAGKDFGSMNMDELLRNIWTAEESNAIAATLTTATTAVPTSNVDAQPQPPPPQQQQQAILRQGSITLPRTLSQMTVDEVWRDIMGFCDEEPPPPPAPAPAQAERQQTLGRMTLEEFLVRAGVVREDMGGQTVVVPARAQALFPQGNVVAPTMQVGNGVVHGVVGQGAGVPMTVAAPTTPGVLNGFGKMEGGDLSSLSPVPYPFDTVTRARKGPTVEKVVERRQRRMIKNRESAARSRQRKQAYIMELEAEVAKLKENNEALQKKQVEMLQKQKDEVIERIEKQLGPKAKRFCLRRTLTGPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJA7</accession>
    <name>F2DJA7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448090</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363975">
        <property type="protein sequence ID" value="BAJ95178.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980794.1">
        <property type="nucleotide sequence ID" value="XM_045124859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJA7"/>
    <dbReference type="SMR" id="F2DJA7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0353220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0353220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0353220"/>
    </dbReference>
    <dbReference type="GeneID" id="123448090"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0353220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0353220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0353220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448090"/>
    <dbReference type="OrthoDB" id="680678at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000911">
        <property type="term" value="P:cytokinesis by cell plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040321">
        <property type="entry name" value="SCD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31762">
        <property type="entry name" value="FAS-BINDING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31762:SF13">
        <property type="entry name" value="OS11G0520500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="430"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ95178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0353220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="711" mass="78556" checksum="EF2545FF473364C3" modified="2011-05-31" version="1">MDAGRAGARRPRPAAAASDPRRAAAAREAMLRMEEVMLAHAGSAGEFSMIIEAPLPNLQRYRRNPTPPAAPSSGSPSRRRGGPHDAGSRDDVPTRLRREGSVTEDIGSARGVRGGEDMGYADDARSRRGTLDARAARPASAGGPEEETEAPVRLRDPRRERGRVSAPPARIVEVQPDVEAVMEEETPLQQLSRGARSSSADRRVEAAVEAEAMAARPSSRGSRREGTGSGENAIVAEPAVEVEADVESVGWSSSHGSEDGREEAVPLAKPLESIITGGRSRSNSPAISRNGVDSGVANRAQSTGRSSFVPPVGANVRPVQAVEVPNGTPRERRTIYPDPTFAQSGRSRDSHDSSTLTEEVEMLKDENVNLLEKLGLAEEKFRQSEARTRELEKQVANLGDGLSMEVKLMKRREEMLVRKEQEIRKALISKNDKSEEITTLQQQLQSASEKAAVSERKLNEAESETKALRTMTQRIILSKEEMEEVVMKRCWLARYWGLAVQYGIYPDISMSKYEYWSSFAPLPLEYVTSAGLRAKDGGSNDLEETDMLVHDLTATAGEGNIETMLAVDKGLKELAFLKVEDAVLIALAQHHRPNVAELSDPDIKSSGDEKFTEAFDLSKEEEEDVLFKQAWLMYFWRRAKTHNVEEDIAEERLQMWVDRHGQQPTSHDAVDVEQGIHELRKLGIEQLLWEFSRQEVNVAERELSDAEDDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMK9</accession>
    <name>F2DMK9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123407113</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365127">
        <property type="protein sequence ID" value="BAJ96330.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956101.1">
        <property type="nucleotide sequence ID" value="XM_045100166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMK9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0706770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0706770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0706770"/>
    </dbReference>
    <dbReference type="GeneID" id="123407113"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0586100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0586100.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0586100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0706770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0706770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0706770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407113"/>
    <dbReference type="HOGENOM" id="CLU_053945_0_0_1"/>
    <dbReference type="OrthoDB" id="1908649at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF5">
        <property type="entry name" value="OS08G0107100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ96330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0706770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="36908" checksum="064750839F332328" modified="2011-05-31" version="1">MECEPEELQFLGPVGIYRESVAILRAHRPLYARIAAAFVLPLSALFLAHIAISHALFSTIDSDDSALESSAPGTASQQRILQRLGADWAALVLFKAAYLLALLLFSLLSTAAAVFSVASVYSAKHDALTFPRVLSVVPRVWRRLAATFLAAFALLFAYHLVFVVVFIALLVATDNGSTLAGLLAFVVAIAYLVGLVYLSVVWHLASVVSVLEDYKGFQAMRKSKALIQGKLWTVVFIFVTLNLVFVVVEFAFRAWIVQGARHGLGAGGRLLLGLVILAALCSVVMVALVVQTVVYLVCKSYHHESIDKSNISDHLEVYLGDYVPLKASDVQMQHFGDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMR7</accession>
    <name>F2DMR7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123448220</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365185">
        <property type="protein sequence ID" value="BAJ96388.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980984.1">
        <property type="nucleotide sequence ID" value="XM_045125049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMR7"/>
    <dbReference type="SMR" id="F2DMR7"/>
    <dbReference type="STRING" id="112509.F2DMR7"/>
    <dbReference type="ESTHER" id="horvd-f2dmr7">
        <property type="family name" value="Hydroxynitrile_lyase"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_63777.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0357170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0357170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0357170"/>
    </dbReference>
    <dbReference type="GeneID" id="123448220"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0297980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0297980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0297980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0357170.1">
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    <dbReference type="KEGG" id="hvg:123448220"/>
    <dbReference type="OMA" id="EKFVPNI"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DMR7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080032">
        <property type="term" value="F:methyl jasmonate esterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080031">
        <property type="term" value="F:methyl salicylate esterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009694">
        <property type="term" value="P:jasmonic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009696">
        <property type="term" value="P:salicylic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000051">
        <property type="entry name" value="(S)-hydroxynitrile lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045889">
        <property type="entry name" value="MES/HNL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992:SF1008">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992">
        <property type="entry name" value="METHYLESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="12"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ96388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0357170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="267" mass="28973" checksum="A110A1E85CFE0E34" modified="2011-05-31" version="1">MEADSGAKAKHIILVHGVCHGGWSWYKVAAGLRSAAGHGYRVHAPDLAASGIDDRRLPEVATFSEYTGPLLDALRSLPAGEKAVLVGHSLGGLSVALAAEMFPDKVALAAFLSAYMPDCASPPSHVLIQHGAGNWVSPLDNEMKPQDADGRLPASFMFGPQFIEQKLYQLCSPEDITLAKSLIRVGSLFLEDLQARQPFTKERYGSVRKVYVVCKQDVTIPEAYQRSMVANNPVDEVREIDGADHMAMLSAPDQVVKCIVDIVEKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2DU36</accession>
    <name>F2DU36_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123412612</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367404">
        <property type="protein sequence ID" value="BAJ98607.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961501.1">
        <property type="nucleotide sequence ID" value="XM_045105566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DU36"/>
    <dbReference type="PaxDb" id="4513-MLOC_79687.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063590"/>
    </dbReference>
    <dbReference type="GeneID" id="123412612"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0050600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0050600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0050600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412612"/>
    <dbReference type="eggNOG" id="ENOG502RY1F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098831_0_0_1"/>
    <dbReference type="OMA" id="KEIDIGW"/>
    <dbReference type="OrthoDB" id="779714at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DU36">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008390">
        <property type="entry name" value="AWPM-19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33294">
        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33294:SF3">
        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05512">
        <property type="entry name" value="AWPM-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ98607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="171" mass="17887" checksum="5DCB28B1BA339290" modified="2011-05-31" version="1">MASGGLKSMASGFLFLNLIMYVVVAVIAGWAINYSIDNSAHSLKGASPPVRLFPIYFPMGNLATGFFVIFALLASAVGISTSLTGLRDVTEGYPASMMSAAASALVAWTLTLLAMGLACKEISIGWRPPSLRALETFTIILAVTQLLCVGSLHAGANAAIMQTPMGMRARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2DUR6</accession>
    <name>F2DUR6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123412089</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role in vesicle trafficking.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK367634">
        <property type="protein sequence ID" value="BAJ98837.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960971.1">
        <property type="nucleotide sequence ID" value="XM_045105036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DUR6"/>
    <dbReference type="SMR" id="F2DUR6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701640"/>
    </dbReference>
    <dbReference type="GeneID" id="123412089"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0582020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0582020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0582020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0701640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412089"/>
    <dbReference type="HOGENOM" id="CLU_067947_1_0_1"/>
    <dbReference type="OrthoDB" id="845153at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001596">
        <property type="entry name" value="vesicle-associated protein 4-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF58">
        <property type="entry name" value="VESICLE-ASSOCIATED PROTEIN 4-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MSP" evidence="5">
        <location>
            <begin position="41"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ98837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25374" checksum="C1C9F3FC2A376EDC" modified="2011-05-31" version="1">MPLWGTASGPPPPVAEGGAAEGSGGSSGAAAIRSLLPTRRRLRLDPPSKLYFPYEPGKQVRSAVRIKNVSKSHVAFKFQTTAPKSCFMRPPGGILAPGETIIATVFKFVEHPENNEKPLDQKCKVKFKIISLKVKGPVEYVPELFDEQKDQVAVEQILRVVFLDAERPSAQLDRLKRQLAEAEAALEARKKPPEDNSPPIVGEGLVIDEWKERRERYLARQQVEEVDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DYG2</accession>
    <name>F2DYG2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123402029</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Binds to the hexamer motif 5'-ACGTCA-3' of histone gene promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds DNA as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK368931">
        <property type="protein sequence ID" value="BAK00134.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951819.1">
        <property type="nucleotide sequence ID" value="XM_045095884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DYG2"/>
    <dbReference type="SMR" id="F2DYG2"/>
    <dbReference type="STRING" id="112509.F2DYG2"/>
    <dbReference type="PaxDb" id="4513-MLOC_58614.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573350"/>
    </dbReference>
    <dbReference type="GeneID" id="123402029"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0475320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0475320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0475320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0573350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573350"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRKF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="YDMAELD"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DYG2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000019">
        <property type="entry name" value="BZIP family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693:SF22">
        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="153"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DOG1" evidence="13">
        <location>
            <begin position="218"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="103"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK00134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="460" mass="50155" checksum="1EFBF370B56F1B86" modified="2011-05-31" version="1">MGDRPWQQPHEHASCSAQAGMIQASTAATSSIHGSIMRKDPGGYEDLAELDQALFLYINSQDQQPIQEQPQTLNIFPSRPMHVVAEPSPKAATSASNIIAASSNLKLQRPPSNSKQSSMPAPGGKATVKREGSGSGGAGTPSTSEHEGPRTPDAKTLRRLAQNREAARKSRLRKKAYIQNLETSRVRLSQMEQEMQRCSAQGAILGGGAGIGGLSPEAAWFDGEYARWVDEHDRMMRHLRAAVDAEGVEHDAAAADGEQLLRQLIDAAAAHHVVLAELKSAVARADVFHLVSGTWLPAAERCFIWIGGSRPSDLIKVMARHMEPVTEQQAAGMYDVQRWAQEREEALDRELQATYRSLSDTVSSDALISPYPDTAAYMAHMSLAISNLSSLEAFVRQADALRLQTLHRLPQVLTARQSARCFLAVADYSQRLRALSSLWLARPRQDQPAQPGAGGRLFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2E2A6</accession>
    <name>F2E2A6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123427751</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370277">
        <property type="protein sequence ID" value="BAK01478.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967805.1">
        <property type="nucleotide sequence ID" value="XM_045111870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E2A6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194120"/>
    </dbReference>
    <dbReference type="GeneID" id="123427751"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427751"/>
    <dbReference type="HOGENOM" id="CLU_023808_1_0_1"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017121">
        <property type="term" value="P:plasma membrane phospholipid scrambling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK01478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="360" mass="40044" checksum="8AA6EED2DF3BCABE" modified="2011-05-31" version="1">MRRLPGLLFQAAAAGRAAARASSSHVSGSRGFSSGGGGSPLPRGWLRRLWIEELKREKEAARKLRVCNAAAKAGGAGEDPLGYLANAARAFVNSGSAAAGVDKVRSGGAARGEAAPSKGGQYDPKDLPYLEAKLEPLLSRANLLIARDVEWANIMFAFEQESRYIIMDPLFSQSPVGYIREKSNVIFRQLLRSRRPFTAQFTDAMGNEIFTVRRPFWFINSSIYAEVDGKEVGVVHRRWHLWRRIYDLYLGNKQFAVVENPGFWNWTFTLIDEDENVLAQIDRNWRGIGFELFTDAGQYAIRFGDDGQSSRLGLDIEKLHVVRPLSLPERSVALALAVSLDSDYFSRRGGWGLPFLIATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2E9W4</accession>
    <name>F2E9W4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chalcone--flavanone isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123410482</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the chalcone isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK372939">
        <property type="protein sequence ID" value="BAK04136.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959363.1">
        <property type="nucleotide sequence ID" value="XM_045103428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E9W4"/>
    <dbReference type="SMR" id="F2E9W4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0662560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0662560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0662560"/>
    </dbReference>
    <dbReference type="GeneID" id="123410482"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0549680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0549680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0549680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0662560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0662560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0662560"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWEB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MRVKNLD"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E9W4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.890.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016087">
        <property type="entry name" value="Chalcone_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016089">
        <property type="entry name" value="Chalcone_isomerase_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284:SF1">
        <property type="entry name" value="CHALCONE--FLAVANONE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16035">
        <property type="entry name" value="Chalcone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chalcone isomerase" evidence="3">
        <location>
            <begin position="232"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK04136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0662560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="417" mass="46312" checksum="FE76864CC88B254B" modified="2011-05-31" version="1">MKHDRLIFANIGHGGGYMYQYPPEFPMSHDLGLSLVTHLGRLLGSSLQHRREICSSGNVKAQEAFICLRKFARAFFFRFSRAYDPKSPHGFSHIKRVTSGVQNLVGSQFGSLKDGHAVQLLLARLAQATVGRLWNDIEQQHASNLLTMAGFAAIVPPFENISPKMLAELIKFGNVDGYINMPADQPCLDRKRLSCSSVAVPTTIFQEDAVEPKTGIKFPAFLEDDSCPATTVLVGVGFKGMKIMRVKNLNLYAFGLYMQPNSICEKLGPKYASVPTTKLKDDPDFYNDLLREDLHIRVRLVVNYKGLSIGAVRDVFEKSLGLRLRKINPNTDYHCLKTFGSYFTKDIPIPAGTKIDFCQTSDGQLITEINGRQISAVQSKDLCRAFFDMYIGDSPVSLEAKREVARNVAGLMGRCGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2EEL1</accession>
    <name>F2EEL1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123398767</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK374587">
        <property type="protein sequence ID" value="BAK05783.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949449.1">
        <property type="nucleotide sequence ID" value="XM_045093514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EEL1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000870"/>
    </dbReference>
    <dbReference type="GeneID" id="123398767"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0000450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0000450.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0000450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0000870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398767"/>
    <dbReference type="HOGENOM" id="CLU_167986_1_0_1"/>
    <dbReference type="OrthoDB" id="652307at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EEL1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021775">
        <property type="entry name" value="DUF3339"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33128:SF45">
        <property type="entry name" value="OS05G0103300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33128">
        <property type="entry name" value="OS05G0103400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11820">
        <property type="entry name" value="DUF3339"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK05783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="72" mass="8019" checksum="ACFA3671088569D1" modified="2011-05-31" version="1">MQDWSPVLISFMLFILLSPGLLFQIPGKSRTIECGKFHTSVVSILIHTIIFFALDAFFLVVVGVRIEFGSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2EI22</accession>
    <name>F2EI22_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123411662</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK375799">
        <property type="protein sequence ID" value="BAK06994.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960563.1">
        <property type="nucleotide sequence ID" value="XM_045104628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EI22"/>
    <dbReference type="SMR" id="F2EI22"/>
    <dbReference type="PaxDb" id="4513-MLOC_73087.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657390"/>
    </dbReference>
    <dbReference type="GeneID" id="123411662"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411662"/>
    <dbReference type="eggNOG" id="KOG1444">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045047_0_0_1"/>
    <dbReference type="OMA" id="HMVTKLG"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EI22">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005338">
        <property type="term" value="F:nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015780">
        <property type="term" value="P:nucleotide-sugar transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="17"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK06994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="342" mass="37040" checksum="FBACB983C881331A" modified="2011-05-31" version="1">MAKPAYATSSLVLGYALCSSLLAIINKYAITKFSYPGLLTALQYLTSVVGVWSLGKLGFLYHEPFNFQIAKKYAPAALVFYLAIFTNTHLLKHANVDTFIVFRSLTPLLVAVADTTFRKQPCPSKLTFLSLVIILGGAVGYVMTDSGFTLTAYSWAVAYLITITTEMVYIKHMVTNLGLSTWGFVIYNNLLSLLMAPVFGVLTGEHLSVFRAIESRGQSWFELDAFIAVALSCVFGVLISFFGFAARQAVSATAFTVTGVVNKFLTVAINVMIWDKHANAFGLVCLLFTLAGGILYQQSVTARGNTPAHREAVANKGCGGDDGTEFDPEKQGLVSSPKDSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>M0W8U7</accession>
    <name>M0W8U7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123398119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044948559.1">
        <property type="nucleotide sequence ID" value="XM_045092624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W8U7"/>
    <dbReference type="FunCoup" id="M0W8U7">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W8U7"/>
    <dbReference type="PaxDb" id="4513-MLOC_4507.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481640"/>
    </dbReference>
    <dbReference type="GeneID" id="123398119"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0399230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0399230.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0399230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0481640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398119"/>
    <dbReference type="eggNOG" id="ENOG502S25C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W8U7"/>
    <dbReference type="OMA" id="GYFSCTY"/>
    <dbReference type="OrthoDB" id="1933825at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044246">
        <property type="entry name" value="ZFP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47287">
        <property type="entry name" value="C2H2 AND C2HC ZINC FINGERS SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47287:SF15">
        <property type="entry name" value="ZINC FINGER PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="124" mass="13457" checksum="69E069034846FC7E" modified="2013-04-03" version="1">MEFNGGEGEGDIDLNLSLHRASSPEPLGYFTCTYCDKKFYSSQALGGHQNAHKFERSVAKRTRELAAARRHGHAGLVGAGEEASRRGKDPTSNAAGSSSHRRASSPETARRDLITEEIDLSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0YHE5</accession>
    <name>M0YHE5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123449706</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045127016.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407900.1">
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407900.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407900.2"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407900.3"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="YbiR_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="28"/>
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            <end position="204"/>
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        <location>
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            <end position="517"/>
        </location>
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        </source>
    </evidence>
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    </evidence>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>M0YK33</accession>
    <name>M0YK33_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0YK33"/>
    <dbReference type="SMR" id="M0YK33"/>
    <dbReference type="STRING" id="112509.M0YK33"/>
    <dbReference type="PaxDb" id="4513-MLOC_7012.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0583690.1"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0703630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703630"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTX3">
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    <dbReference type="InParanoid" id="M0YK33"/>
    <dbReference type="OMA" id="YTGPAPM"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YK33">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="453"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="497" mass="54574" checksum="755547B6508413AD" modified="2013-04-03" version="1">MTAAIQYDNISSIMSVTLWLGDDHGPSYSLSSKVDLKSALPELVAVGFSASTGKSFELHQLLSWHFNSSLQGKTATVVAPPVLPPPSSRTSSSGVVAGAVAGASLFLVVFFSMSAILLRRRHNKKKREAKEEMDSEDEMIMEMEFGTGARRFPYRQLTNATRNFAAEEKLGQGGFGAVYRGHLRELGLDVAIKRFSKESSMQGRKEYMSEINVISRLRHRNLVQLVGWCHNHDELLLVYELMPNRSLDIHLHGKGTFLTWAMRMKIVLELGSALLYLHEEWEQCVVHRDIKPSNVMLDESFCAKLGDFGLARLIDHSVGMQTMTIVSGTPGYMDPQCLITGRASSESDVYSFGVVLLEVACGKRPMSTSANKQGVSRLTEWVWNLYGQGGVLEAVDERLNGHYDEAEVERVMAVGLWCAHPDPSARPSIRTAMATLQSKDAKQPPMLPAKMPVPTYAPPMAPWDGQSSSSGLSASTVTRSSTTSGYTGPAPMVTPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287G7J6</accession>
    <name>A0A287G7J6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123445759</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044978730.1">
        <property type="nucleotide sequence ID" value="XM_045122795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287G7J6"/>
    <dbReference type="SMR" id="A0A287G7J6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0074170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0074170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0074170"/>
    </dbReference>
    <dbReference type="GeneID" id="123445759"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0074170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0074170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0074170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445759"/>
    <dbReference type="OMA" id="DLQFISM"/>
    <dbReference type="OrthoDB" id="10063692at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287G7J6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.40:FF:000004">
        <property type="entry name" value="ataxin-3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033865">
        <property type="entry name" value="Ataxin-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF3">
        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR633865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0074170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="318" mass="34972" checksum="2C61BEE9EF8DF0ED" modified="2017-11-22" version="1">MDAGTGNSGMLYHELQARQLCGLHALNAALQGPFFSEGDLLQIAADLDAREREVMSAAGTVGAGDFLAEGEGSHNVSNSGDFSVEVLKRALEVWNLQFISMYSQAAAGAQSNPELETAFICHFQNHWFCIRNVDGEWYNFNSLYLAPEHLSQFLLSAYLDSMRGPGSNIYVVRGTFPRDCPTDYNDFGQWLDPEEARIITHSRFEAQQKQGSNSAIAMASRRYLTVGVASGLRGLEVPGTKIEVPRQQPYQQKGMTMMQEESDDELKAAIAISLMPFEAPGTSSLPAQEERNLKSALGKDTSMEEPVWSNSEGPVEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287I4I9</accession>
    <name>A0A287I4I9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="4">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123426254</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glycolate + O2 = glyoxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25311"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25312"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Photosynthesis; photorespiration; glycine from 2-phosphoglycolate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044965987.1">
        <property type="nucleotide sequence ID" value="XM_045110052.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965989.1">
        <property type="nucleotide sequence ID" value="XM_045110054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287I4I9"/>
    <dbReference type="SMR" id="A0A287I4I9"/>
    <dbReference type="PaxDb" id="4513-MLOC_16035.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0152860.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0152860.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0152860"/>
    </dbReference>
    <dbReference type="GeneID" id="123426254"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0125770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0125770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0125770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0152860.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0152860.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0152860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426254"/>
    <dbReference type="eggNOG" id="KOG0538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287I4I9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009854">
        <property type="term" value="P:oxidative photosynthetic carbon pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000063">
        <property type="entry name" value="peroxisomal (S)-2-hydroxy-acid oxidase GLO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF107">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 14" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0323">Glycolate pathway</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="285"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="308"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0152860.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="40226" checksum="4ED8641E9125293E" modified="2017-11-22" version="1">MEITNVTEYQAIAKQKLPKMIYDYYASGAEDEWTLQENREAFARILFRPRILIDVSKIDMTTTILGFKLSMPIMISPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKDRRVVEQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFVLPQNLTLKNFEGLDLGKMDQANDSGLASYVAGQIDRTLSWKDVKWLQSITTMPILVKGVITAEDSRLAVENGAAGIIVSNHGARQLDYVPATISALEEVVKAARGQIPVFLDGGVRRGTDVFKALALGAAGVFIGRPVVFSLAVAGEAGVSNVLKMLRDEFELTMALSGCNSLADITRNHVVTEADKFGVMPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287NF05</accession>
    <name>A0A287NF05_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447940</name>
        <name evidence="8" type="synonym">MLO</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in modulation of pathogen defense and leaf cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminus contains a calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MLO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980612.1">
        <property type="nucleotide sequence ID" value="XM_045124677.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980613.1">
        <property type="nucleotide sequence ID" value="XM_045124678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287NF05"/>
    <dbReference type="STRING" id="112509.A0A287NF05"/>
    <dbReference type="PaxDb" id="4513-MLOC_11560.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0347680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0347680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0347680"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0347680.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0347680.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0347680"/>
    </dbReference>
    <dbReference type="GeneID" id="123447940"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0290140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0290140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0290140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0347680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0347680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0347680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0347680.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0347680.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0347680"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQK2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1388414at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287NF05">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF9">
        <property type="entry name" value="MLO-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4 8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0347680.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="514" mass="59580" checksum="D860740545B0752C" modified="2017-11-22" version="1">MEEEGRSLAETPTWSVATVTTLMVATCFLIERSLSRLAKWLRKTKRKAMLAALEKIREELMLLGVISLLLSQTARWISEICVPSTLFTSKFYMCTEKDFDDLDQHGEHTANNTHIARILVGGQSMHVCDEGHEPFVSYEGLEQLHRFLFILGFTHVLYSFVTVVLSMIKIYSWRKWETQACTLSREQLQPRRKVMRRQSTFVFHHASHPWSKSKILLWMLCFLRQFKGSIKKSDYMALRLGFITYHKLPHSYDFHKYMVRSMEDDYNGSVGISWPLWVYAIICIFVNVHGLNIYFWLSFAPVILVLLVGTELQHVIAQLALEVVEATATNVGTQLKLRDDLFWFGKPRVLWWLIQFISFQNAFEMATFLWSLWELSANSCFMKNQYMVVIRLASGLLVQVWCSYSTLPLNVIISQMGSKFKKSLVSESVRDSLHSWCKRIKERRHNPLFMRNGTLTSRSVCSLDTTIYETDHETNTVCTLSRTVSASSLDEALTAITVDDEEEISHIEEDTRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287PGC8</accession>
    <name>A0A287PGC8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123449297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982390.1">
        <property type="nucleotide sequence ID" value="XM_045126455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287PGC8"/>
    <dbReference type="SMR" id="A0A287PGC8"/>
    <dbReference type="STRING" id="112509.A0A287PGC8"/>
    <dbReference type="PaxDb" id="4513-MLOC_37041.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396180"/>
    </dbReference>
    <dbReference type="GeneID" id="123449297"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449297"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287PGC8"/>
    <dbReference type="OrthoDB" id="1921697at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287PGC8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35468">
        <property type="entry name" value="MYOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35468:SF1">
        <property type="entry name" value="MYOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="484"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="394"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="545" mass="60729" checksum="2A66E522B870048E" modified="2017-11-22" version="1">MSARRPARAQHAAATATVTPRILNLARRSSSAAGGRPSRGVARPQQPRPAPQAVNLGALFEMERRVRGLESAPASPPCSSRGPAARPREREDAAEQEEKWRFQAEILRAECNFLRMEREVALRKLDCHRGQMEAALKTAVETLASGRKKIDGSGEVGVAAALEEGIEDLKEMMQELRVEKESGRRAMSGTRELRRSHGRNFDRQASSLRRRLEKMPSADAEPTIKDIREIARPVSPPLPPPADHSDGDDHAPSANLSDVEMLRVKMEGMSNGMRERMAEYSRRLEAVASGGDNAGCQSRKCGSRHSRKASACSQRSWSGSSNASNGSNVAAFRQNASHGGTSVASEKNHPHKIVAEDCKLVRSGSCCDCKEIVGKIMKQVRAESEQWTEMQDMLEQVRLEMQELQSSRDTWQRRAIASDISLGSLNSEMLEWKQRAQASEQHAEELQKKVSELQGKLHTFKSHFPAPNLPSRAWSEACRMENPRAAKAQHHHHRPPQQQQQQQPDCGKEKEKHVLICRVRHSPSVIPRRSPLQDIGNIALPRRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287RAI9</accession>
    <name>A0A287RAI9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123395473</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044946413.1">
        <property type="nucleotide sequence ID" value="XM_045090478.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946414.1">
        <property type="nucleotide sequence ID" value="XM_045090479.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946415.1">
        <property type="nucleotide sequence ID" value="XM_045090480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287RAI9"/>
    <dbReference type="SMR" id="A0A287RAI9"/>
    <dbReference type="FunCoup" id="A0A287RAI9">
        <property type="interactions" value="815"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287RAI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0472920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0472920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0472920"/>
    </dbReference>
    <dbReference type="GeneID" id="123395473"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0391720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0391720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0391720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0472920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0472920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0472920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395473"/>
    <dbReference type="InParanoid" id="A0A287RAI9"/>
    <dbReference type="OMA" id="NFSCFCL"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287RAI9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000061">
        <property type="entry name" value="Probable protein phosphatase 2C 67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF699">
        <property type="entry name" value="INTEGRIN-LINKED KINASE-ASSOCIATED SERINE_THREONINE PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0472920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="360" mass="39089" checksum="3F4CC350867055E0" modified="2017-11-22" version="1">MTHQKREASSSNEDWESKRPKGAATSTEKDCHVETASSQEANGEKRDTSQKGSNLPLDPCISDEKSTTISKVPSQQEMILTSIEADAAEDKGCRHTMEDAWVVLPDASMGSPGNLRCAHFAIYDGHGGRLAAEYAQKHLHPHVIAAGLPRELINGKVAKKAIIEGFRRTDESLLQESAEGNWQDGATAVCVWVLGQTVIVANAGDAKAVLARSTSTDGEGAVADIKNPLKAIVLTREHKAIFPQERSRIQKAGGSVGPNGRLQGRIEVSRALGDRHFKKVGLIATPDVHSFELTMKDHFIILGCDGLWGVFGPSDAVEFVQQQLKETSSATLAVRRLVKEAVRERRCKDNCTAVLIVFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287SDM8</accession>
    <name>A0A287SDM8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">COBRA-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
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    </reference>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
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        <text evidence="1">Belongs to the COBRA family.</text>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23957" checksum="4C3FE13EFAF94F15" modified="2017-11-22" version="1">MAPVSGVAGSSRSAACCAVLLTVVLFFSAPATTEAYDSLDPNGNITIKWDIISWTPDGYVATVTIFNYQQFRHISAPGWQLGWSWAKKEVIWSIAGALATEQGDCSKFKSAPPHCCKRDPTIVDLLPGTPFNQQIANCCKAGVIKTFNQDPGNAASSFQISVGLAGTTNKTVKMPKNFTLKAPDPGYTCGRALVGRPTRYYSSDGRRVTQALMSWNVICTYS</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="GO" id="GO:0010333">
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0046246">
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            <begin position="5"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="207" mass="23949" checksum="77D33C8260EE954D" modified="2022-05-25" version="1">MTEMAGIKKERVRKSILAAGFSSDLPMRMELIDTLQRTGVAYHFGKEIQELLRGMHGDNQGSSDDLHTTAVRFYLLRTHGYHVSPDVFMKFRDDRGNFVSNDADSLLALYNASYLRTRGEEVLDDAIIFTNSRLHCMLEHLEPQLAEEVRCALETPIFRRVERVETRLYIPVYEKKATRDEDILELAKLDFNILQTIYCKELKTLTT</sequence>
</entry>
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    <accession>A0A8I6WA24</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
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    <reference evidence="3" key="1">
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0109790.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="118" mass="14596" checksum="5A08CC665D3D7DB3" modified="2022-05-25" version="1">MYYRWIDTEMPDWAVIEIRERGRRAWASLDLEERREKVEAEEKAEQKKEWEDYCVEQRAFLDEMKRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEAGDGKGKYPRWTQ</sequence>
</entry>
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    <accession>A0A8I6WAM1</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115570.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="156" mass="17322" checksum="8A2343D74CF26C0E" modified="2022-05-25" version="1">MKCKTEPSLAMLHILSSVAFCSGSGMNPPLSLSLSLSPSPYEEEEQQTSEALIADSLTDRLCGCHITSLLLWCCQPAIPLPWIWLWRGLGTVEFCSDVSVCDEVWSADARYEQLPRLSKLLTTMASLMDGWMLLCDEEMEFLPGAMMMTMAEISMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCW4</accession>
    <name>A0A8I6WCW4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hyperosmolality-gated Ca2+ permeable channel 1.4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123436852</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971599.1">
        <property type="nucleotide sequence ID" value="XM_045115664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCW4"/>
    <dbReference type="SMR" id="A0A8I6WCW4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053700"/>
    </dbReference>
    <dbReference type="GeneID" id="123436852"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0042080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0042080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0042080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0053700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123436852"/>
    <dbReference type="OMA" id="FRIWERS"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF98">
        <property type="entry name" value="TO DEHYDRATION PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="571"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="7"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="197"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="10">
        <location>
            <begin position="370"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="733"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="92555" checksum="2C070F75A3A93353" modified="2022-05-25" version="1">MATLQDLGVSAFINILGAFVFLLLFAVLRIQPINDRVYFPKLYIAGKRGAGDHRGARRVINLNLCTYFKFLSWVPGALRMGQTELIHHAGLDSAVYLRIYTLGLKIFLPVMVVALLVLIPVNVSGGTLLNLRKDVMFSDIDKLSISNVSPGSNRFFIHLLMAYVFTFWTCFMLYKEYSNVAFMRLHFLASQKRCADQFTVIVRNIPRVSSHSTSETVDEFFRRNHPDHYLGQQAVYNANRYAKLVKQKERLQNWLDYYQLKFERHPDKRPTGRTGCFGFCGRQVDQIDYYRATISELDKRIASERQRVLNDPKAVMPVSFVTFDSRWGAAVCAQTQQSKNPTQWLTNWAPEPRDVYWQNLAIPFFSLSIRKFLISIAVFALVFFYMIPIAFVQSLANLEGIEKVAPFLRPVIEVKVVKSFLQGFLPGLALKLFLYILPTVLMIMSKVEGYVSLSSLERRTASKYYYFMLVNVFLGSIIAGTAFEQLDSFFHDPPSQIPRTIGVAVPMKATFFMTYIMVDGWAGIANEILRVKPLVIYHLKNMFIVKTERDRERAMDPRSIALGENLPSLQLYFLLGLVYAVVTPILLPFIIVFFAFAYLVYRHQIINVYNQEYESAAAFWPQVHSRIIASLLISHVTLFGLLSTMKAAYSTPLLIFLPMLTLWFHKYCKSRFEPAFRKYPLEEAMEKDVMEHASEPSLNLKTYLANAYLHPIFHMFEQEDQKEAATIEVRIDKSEHQQQQHVRSSHSQYEEETSAQTHYHHEEMTSSQYQYQYQYQHEQTHMRSEQSPPHFVYHHGDEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDD2</accession>
    <name>A0A8I6WDD2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123403364</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953241.1">
        <property type="nucleotide sequence ID" value="XM_045097306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDD2"/>
    <dbReference type="SMR" id="A0A8I6WDD2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000540"/>
    </dbReference>
    <dbReference type="GeneID" id="123403364"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0000540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403364"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF531">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035297002" description="Protein kinase domain-containing protein" evidence="17">
        <location>
            <begin position="24"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="303"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="422"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="82808" checksum="477A42D718F63924" modified="2022-05-25" version="1">MQPLLRLGLGLALLAVAAQYATGTVAPGSECRRWCGDVEIPYPFGIDPGCSLGEDFDISCKVQDGIRKPFKYTLEVLSISLTNSTARVLNNIMGYCYNNSTTSMEEFGNSMDVGEISPSPSFPYRLSDVHNKFTVIGCNALAFMSDTDDTGTGYQAYGVASCRGLTDLVDGSCSGIGCSQTTIPKKMRNYYTDFLKSVNTSKIWEFNPCSYAVLMETTRFNFSTSYISNTVFNDTYLGRVPMVVDWAIRNVSSCEAAQRNNTSTYACLSSSSKCVNSTNDLGYTCSCIPGYDGNPYLLDGCTDVNECKDHPCPSDGFCHNTDGGYWCSCGQGKVFAYKSNTCNPDTRFIIGVTIGLFGLMVIIMATIFWGQMICQKRKLSKVKQEYFRQHGGVLLFDKMKSEKGLAFTVFSEDELIHATNNFDNSRILGRGGHGTVYKGIMKDKMPVAIKRCGLVDERQKKEFGQEIIILSQINHKNIVKLLGCCLEVEVPILVYEFVLNGTLFELIHGENRLLKISFSTLLRIAHEAAEGLSFLHSYASPPIIHGDVKSSNILLDNNYMAKVSDFGTSILAPSDKEQFVTIVQGTCGYLDPEYMQTCQLTDRSDVYSFGVILLEILTGQLPLKLEGSETQRSLSSVFLSAMKKNNLDDVLASHVKGQESMELLTGLADLAKKCLDMHGINRPSMKEVADELNKLRKLSLHPWLQLDMETDAESLLGGESTSGHEIELSGYTIIGENENQPINTKGSCYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WF01</accession>
    <name>A0A8I6WF01_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123450220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983518.1">
        <property type="nucleotide sequence ID" value="XM_045127583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WF01"/>
    <dbReference type="SMR" id="A0A8I6WF01"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087600"/>
    </dbReference>
    <dbReference type="GeneID" id="123450220"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0072140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0072140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0072140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0087600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450220"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1000.10:FF:000003">
        <property type="entry name" value="Guanylate-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000723">
        <property type="entry name" value="Guanylate-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1000.10">
        <property type="entry name" value="Guanylate-binding protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="7">
        <location>
            <begin position="62"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="951"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="546"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="620"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="892"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="956"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1004"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1066" mass="119849" checksum="7A7AA33965EACCB7" modified="2022-05-25" version="1">MLQRLGLRGSPSAAAAADASASPAAPAAAAGGLGRPLRLVYCDDKGKFVMDPEAVAALQLVKGPVGVVSVCGRARQGKSFVLNQLLGRSSGFQVASTHKPCTKGLWMWSAPLKRTGLDGTEYNLVLLDTEGIDAYDQTGTYSIQIFSLAVLLSSMFIYNQMGGIDEASIDRLSLVTEMTKHIRVRASGGRSSASELGHFSPVFIWLLRDFYLDLSEDNRKITPRDYLELALRPVQSGGRDVSSKNGIRESIRALFPDRECFTLVRPVNNEKDLQRLDQLPMNTFRPEFTSGLDAFTKFVFDRTRPKQLGASTMTGPILAGLTQSFLDAINTGAVPTISSSWQSVEEAECRRAYDSAVDTYNSSFDCKKPAEEDALREAHERAVNKAISVFNASAFGAGSARSKFEKLLQTTLRKRFEDFKRNAFLEADLQCSNKIQSMESKVRAACNRPDSKLDDVVRLLDGLLMEYEATSYGPKKWTKLVTFLHQCLAGPMLDLFRKQLEHIDAERNALRLKCSSSDDKLSLLRKQLEASEGHRAEYLRRYEETINDKQKMSKDYSVRIAELQNKVSKLDERCKSLSSALEQAKRECADWKSKYDHCISQQKADESKLKSLIASLESRVSISEGRLSAVREQAESAQEEASEWKRKYEYAANEAKTALERAASAQERTNKKVQEREDALRAELATQLHEKEELISTLNAKINKTEVHKTSLMSRLEVTEAKLKNHESDSVALKEEIRLLTDNLESIKTEAQSREKEVGILEQEKNHLQEKFLSECKKFDEADMRCKDAEREAKRAVELADLARVEAAAAQRDKGEAQRLAMERLTLIERMERQVETLEREKSKMVEEMERLHQSELDALSKVRMLDERVDEREKQIGEMLEQNNQQRSNTVQVLESLLATEREACAEANKRAESLSLQLQSTQGKLDMLQQELTSVRLNETALDSKVKASYSRRARGEATESVHYMDVDDDDTGRRRKRSKSTTSPFKNNHTEDGGSVFFGEDTNNGSQQLEGTETETEDYTKFTVLKLKQELTKQGFGAQLLQLKNPNKKDIVALYEKHIVGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WH02</accession>
    <name>A0A8I6WH02_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PROP1-like PPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123398673</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
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    <dbReference type="RefSeq" id="XP_044949090.1">
        <property type="nucleotide sequence ID" value="XM_045093155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WH02"/>
    <dbReference type="SMR" id="A0A8I6WH02"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0011250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0011250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0011250"/>
    </dbReference>
    <dbReference type="GeneID" id="123398673"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0008370.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0008370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0011250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0011250.1.CDS1"/>
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        <property type="component" value="Chromosome 1H"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
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        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="191"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="369"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="474"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="509"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="545"/>
            <end position="579"/>
        </location>
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        <location>
            <begin position="580"/>
            <end position="614"/>
        </location>
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        <location>
            <begin position="615"/>
            <end position="649"/>
        </location>
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        <location>
            <begin position="650"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="PROP1-like PPR" evidence="5">
        <location>
            <begin position="698"/>
            <end position="842"/>
        </location>
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        <location>
            <begin position="720"/>
            <end position="754"/>
        </location>
    </feature>
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        <location>
            <begin position="755"/>
            <end position="789"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0011250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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    <name>A0A8I6WJI3_HORVV</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC123421428</name>
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    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the protease inhibitor I20 (potato type II proteinase inhibitor) family.</text>
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    </dbReference>
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        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="8318" checksum="012A7535F809ABA4" modified="2022-05-25" version="1">MATSRLHIACALLLIGVVLLGQNQEGMEAVACPQYCLEVDYVTCPSSEQQKLPARCNCCMAPKGCTLHLSDGISQTCS</sequence>
</entry>
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    <accession>A0A8I6WKQ4</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKQ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0210700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="26092" checksum="6F83089558693EAE" modified="2022-05-25" version="1">MPTTCYEQVLAHGTTELKFVYTNDSVMVPSFLFYFHERLQEEEENENFMELDLEYTANQQGVVVIQLCFKRHVMVFQWASSDKHYPELMDFLRSGIHFATVDIRNDKLKMRHNFGIEIPADCHIDIQDKFKLEHERTLMAHMAVALIDEDYADMNTKFPRAYFQLKSGRGPHLTVSTLSMQQKMHTYVSYELYCMIRIMNYGQCHLAPAPAPRVWGLFDSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WL72</accession>
    <name>A0A8I6WL72_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123431250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971010.1">
        <property type="nucleotide sequence ID" value="XM_045115075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WL72"/>
    <dbReference type="SMR" id="A0A8I6WL72"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213050"/>
    </dbReference>
    <dbReference type="GeneID" id="123431250"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0177190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0177190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0177190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431250"/>
    <dbReference type="OMA" id="EIFSRWF"/>
    <dbReference type="OrthoDB" id="407275at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035205422" description="FAD-binding PCMH-type domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="75"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="535" mass="58140" checksum="0478CD9E4714441E" modified="2022-05-25" version="1">MGMLRGLALAFSVSFFSCYLAMVSSDVASSDSDGDGFLQCLREKIPRELVYTQSSSNFTDVLVSSIKNPRFFTNATARPLCIVTPGDASHVQAAVVCGRMQGVRLRVRSGGHDYEGLSYRSARPSEVFAVVDVGASLRAVRVNRLESTAWVDSGATIGELYYAIAKNDSLVAFPAGECTTVGVGGHFSGGGIGMMMRKHGLAVDRIVDAKLVNADGDLLDRAGMGEDLFWAIRGGGGGSFGVVLSWKVQLVNVPPTVTVFNIGKTVDQGAVDILTRWQDVAPSLPSDLTIRVIVQQGQQALFQGQYLGACGALVETMGEQFPELGMTSADCQQMTWLQSAATPFISFSSNGTLEEVLLNRTASLSTSTKGKSDYVRRAITKAAWEEIFSRWFAMDGAGRLILEPHGGFMDTIPAAATPYPHRNGVLYVIQYMAFWQQQGEGGAAAKSWIGGLYDFMGQHVSKNPRRAYVNFRDLDIGQNDDDGTFDFENGPLWGERYFVGNYRRLASVKSVVDPTNYFRNEQSIPPLIETTTQVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMR6</accession>
    <name>A0A8I6WMR6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMR6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0087940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="148" mass="17704" checksum="E43C16D051061CB5" modified="2022-05-25" version="1">MKFFMCANYESDPPESISAYVGPPSPPPLCMHYRWIDTEMPDWAVTEIRERGRRAWASLDLEESREKAEAEEKAEQKKEWEDYCVEQRAFLDEMIRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEAGDGKGKYPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WN92</accession>
    <name>A0A8I6WN92_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="8">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123424563</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044964130.1">
        <property type="nucleotide sequence ID" value="XM_045108195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WN92"/>
    <dbReference type="SMR" id="A0A8I6WN92"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106750"/>
    </dbReference>
    <dbReference type="GeneID" id="123424563"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0106750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Alpha-glucan phosphorylase 2, cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF4">
        <property type="entry name" value="ALPHA-GLUCAN PHOSPHORYLASE 2, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00343">
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    </dbReference>
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    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="474" mass="54892" checksum="02D9FA0BAC8EE7AB" modified="2022-05-25" version="1">MATLNLPSWGYGLRYRYGLFKQRIAKEGQEEIAEDWLDKFSPWEIVRHDVVYPIRFFGHVEISPDGRRKWAAGEVLNALAYDVPIPGYKTKNAISLRLWDAKATAADFNLFQFNDGQYESAAQLHSRAQQICAVLYPGDATEEGKLLRLKQQYFLCSASLQDIIFRFKERKPDRVSGKWSEFPSKVAVQMNDTHPTLAIPELMRLLMDEEGLGWDEAWDVTNKTVAYTNHTVLPEALEKWSQAVMRKLLPRHMEIIEEIDKRFREMVISTRKDMEGKIESMRVLDNNPQKPVVRMANLCVVAAHTVNGVAELHSNILKEELFADYLSIWPKKFQNKTNGITPRRWLRFCNPELSEIVTKWLKTDKWTSNLDLLTGLRKFADDEKLHAEWAAAKLASKKRLAKHVLDVTGVTIDPNSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKEMSAEERQKVTPRTVMNVNPAMALVDWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WND2</accession>
    <name>A0A8I6WND2_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WND2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0107780.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0088370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0088370.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0088370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0107780.1">
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="term" value="P:cell fate determination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="TPD1"/>
    </dbReference>
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        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33184">
        <property type="entry name" value="PROTEIN TAPETUM DETERMINANT 1-LIKE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="TPD1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="14563" checksum="090EECB114582C9D" modified="2022-05-25" version="1">MPASLNLVSFVHAAVGADVPHRVSLGCAGAADIAIEQGPGTTLPSGIPSYTVDIINRCSGAGRRADCAISGIHVRCGWFSSVTLVEPTKFRRVAPDDCLVNDGKPLLADDTISFEYANSFAYKLSVAKASCVHPAADTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQV9</accession>
    <name>A0A8I6WQV9_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">pectinesterase</fullName>
            <ecNumber evidence="3">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQV9"/>
    <dbReference type="SMR" id="A0A8I6WQV9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0251060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0251060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0251060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0251060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0251060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0251060"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046910">
        <property type="term" value="F:pectinesterase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000029">
        <property type="entry name" value="Pectinesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158825" description="pectinesterase" evidence="7">
        <location>
            <begin position="43"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="8">
        <location>
            <begin position="75"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0251060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="41919" checksum="67BAD9136D00D993" modified="2022-05-25" version="1">MYGHDTIPSAPSNYTASEMGTRSSLVLLPLAILLLNNSHASAVQTLAGDTISMPLWVHPRERQLLDLKVGDMQVDMVVSQDGSGHFSTVSEALEAARPVRGKMYVIYIKGGIYREEVVIRKNNIALIGDGINKTVITGNKSRQGDGWASYDTAVVYAKGKMLLARDLTVENTAGREAGAAVALRSASKQSVFYRCEFKGYEDTLLADSNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSFQNCRVTSQEDLNGSETETYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWNKTNGDVPPTVFYGEYGNTGPGANLSKRVPWLHVMDAGQAEHYTVKRLIRGDEWLNATGVVYQPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WRA6</accession>
    <name>A0A8I6WRA6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123429434</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="SMR" id="A0A8I6WRA6"/>
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        <location>
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            <end position="168"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0111370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="367" mass="40301" checksum="E877C867CC1473D0" modified="2022-05-25" version="1">MSDVTAVMDLGVEEPQLSLPPGFRFHPTDEEVVTDYLTRKVLHESFSCQAITDVDLNKNEPWDLPGLAKMGEKDWFFFVHKGRKYPTGTRTNRATERGYWKATGKDKEIYRGKVLVGMKKTLVFYTGRAPSGGKTPWVMHEYRLEGQLPHRLPPTAKDDWAVCRVFNKDMAAAKNAPAQMAPVANGAMEDPLAFLDVDGFVNYDELLNNAGLLDDADLPMLMDSPSASGADDFAGASSSTTSTALPFDLDTEHLTIKTEPPVPQQQMQSPNYFFMPATTANGNHGGAGYSPYQGAMGVDQQTAIRRHCKPEVASSSALLSPSLGFFDTCALAGAADTSFPMMPSSRSYVDLEELCRGEPPMDFSNMW</sequence>
</entry>
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        </recommendedName>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973185.1">
        <property type="nucleotide sequence ID" value="XM_045117250.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="123440694"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440694"/>
    <dbReference type="OMA" id="QTHFEFA"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SLX8"/>
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        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
    <sequence length="252" mass="28236" checksum="AC73E1B876D9A3CC" modified="2022-05-25" version="1">MNRLVGSGIGSADVPPPQHQQMEDVMGLSDPMLLELRNNYRQRIIEYARKAVIARLDARSRTPADWQRRLPELAWRVEKVLFEKHPNKREYYNMTNGPVQTHFEFAVTAALAQIQRQEHGRNQQMSRQRASPTAYAPGGMIPAPGVTQGEAPNEHVNTVLSLGINSSDAHSEVSNSLLAVCAPVNKEVSKEPKFSCPFCFDELVDASSTNCGHIFCLECIKTSIQAQNRCPACWRALTMNSFHRVYLPATMD</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC123427849</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="4" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967919.1">
        <property type="nucleotide sequence ID" value="XM_045111984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXD4"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196160.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196160"/>
    </dbReference>
    <dbReference type="GeneID" id="123427849"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0162780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0162780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0196160.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196160.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427849"/>
    <dbReference type="OrthoDB" id="625046at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47127">
        <property type="entry name" value="10A19I.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="286"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196160.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="568" mass="62436" checksum="5322EBAA6CA75ED9" modified="2022-05-25" version="1">MNLVQHADNMPGNNGSGKQAVWTTAMSSFVLKFMANLVTSGTRASNSFRQVHHKSCAKALNERFKLQVSAAQISNHLRKWKRIWGRVNKLKTLSGALWDEHTCTVVLDQEHYASHVKDHHSDADLLNTPIEHYHEMATICGSGMVSGVYASRNSNDLLSTDVADDEFENEDSNGESDPLATYVTENEMENRKTNGVSDPLATDVSENEKTCEEAMKSFTNTDGGNFGDPSGSLPPPKKAKMSHLNLSQQCGALVTQPGVAISHGMNMEQHADNMGGTNGGGGQLVWTNAMSSFVLQFLTNLVVGGTRPSHVFKQVNLKSCAQALSEHFRVQVNSAQISNHLRKWKKIWKKVNILKSLSGALWDANTSTIVLNHEHYTAHVKDHHHDADFLNTPIKNYREMATIFVKGMASSRPARSSNESLAREPENVIENENVNGGGGLIIAKGVEGAAMSFTNTDEGDSGDSSGSLPPPPPPKKAKVKNDDSLLCTMTRVLDRLAEAVEKCSRRDTDVPDGLWANMKGLPGFEQEHLAHYYAYLCENAHVARAFVKLSVPHKMVWVNRYIKNHLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYH9</accession>
    <name>A0A8I6WYH9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123443417</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975705.1">
        <property type="nucleotide sequence ID" value="XM_045119770.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WYH9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254050"/>
    </dbReference>
    <dbReference type="GeneID" id="123443417"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0210920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0210920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0210920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0254050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443417"/>
    <dbReference type="OrthoDB" id="24822at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000914">
        <property type="term" value="P:phragmoplast assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010245">
        <property type="term" value="P:radial microtubular system formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14010">
        <property type="entry name" value="STKc_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056980">
        <property type="entry name" value="ARM_RUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056981">
        <property type="entry name" value="HEAT_ULK4_RUNKEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044591">
        <property type="entry name" value="RUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562">
        <property type="entry name" value="SERINE/THREONINE-KINASE ULK4-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24970">
        <property type="entry name" value="ARM_RUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23606">
        <property type="entry name" value="HEAT_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="350"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="355"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1344" mass="149246" checksum="5B68FB5E104AFCAF" modified="2022-05-25" version="1">MNNFHVYEAIGRGKHSTVYKGRKKKTIEYFAVKSVDKSQRSKVLNEVRMLHSLDHPNVLKFFSWYETSAHFWLVLEYCVGGDLKGLLEQDKKLPESAMHDLAYDLVKALLFLHSQGIIYCDLKPSNILLDEFGCMKLCDFGLARRLKDIEKTDPGDVPQPMRGTPCYMAPELFQEGGVHSYASDFWALGCVLYECYAGRPPFVGREFTQLVKSILSEPAPPLPDNSSRSFQNLINCLLMKDPAERLQWSELCAHNFWRNSMPMIPLPPQPAFDNMVGLPTTPYLSERNDDKPSRQLTPPKTREHLRKKDENSAKVFTTPVKNVQTGKKNNAKPCKADGLKGVNVLRMSRIAKKNLQREKDKENYRRPPTETDENEAEVKIENNDMELDFGENPEGDTPDDNDASDNAGSTADEKHTAEGTDGNEENCINQVDMLTDECSIKPDTMLKAGQNCSENLDVVATPPSFCMRKARPKITSGAATGSEPSNIFEAFWHPTDLAVKPVMPSKKGDKATEAVPVLPFEALPAADYIRLPREQMNAFNSQIIQSLSGSFQVSEKQNIIRYLELLSMNSDAANVITNGPIMSLLIKMLRLSKTSVLRVQVASLMGLLIRYSTILDAELASSGIVNALSDGLRDRHDKLRRFCMATLGELLFYISTQSDQDSKESNAQESPMKDNKSGALWQVPSSVIALVSSILRKGEDDLAQLYALRTIDNICSQGTDWTSRFASQDVIGHLCYIYKATGKQENTRLIAISCLSRLARFSSSCTHLILEKLSFKDIACTLLKGNPREQQISLNLLNSALGNSHIIPNMSRYILSLTEEKQLVPGLISLIEQGADILRGKTLLFVALLCKNSRRWLPQFFCNAKLLSAVDRLGKEKDGFIHQCTEAFVQLIASLVPGILDTVSSDIQQVMGGKRHGPIIALTGRAHPKSTIHLFPVILHLLGSVSFNHRVVTSQVLLQLANLMKILETPFQARDDFQMTLLRVLEAATEEPSVILREHKIFTSRFLPSLSILYKGNKDCDARFLCLKILSDVMIVIFSDSSFTADEQCLADLKTISHKYFLPMYPSFAEDEDPIPMYAQKLLVMLMEHDCVKVSDILNEATVSQCFEFLLGDLSNANVSNVKLCFALASAPDMDSNILSQLQVVRRIGNLLEFVTAKDMDDFLEPTLELCRAFIIRGTGSNRSIVLTKEPALLVDSAFSMSVAVDQQSCIMDICDLGGSMGIFLEVVGNSDPQISDLASDCVVLLLKAAPREATMGLLTNLPKLSALLDLLKHGASLLLARLLYSLAFSCRQYLTQGMILSISVPALMRVEALVSSFKGSQDSCLADAASCVGAELQRLPRCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZS8</accession>
    <name>A0A8I6WZS8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123452136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984668.1">
        <property type="nucleotide sequence ID" value="XM_045128733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZS8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093060"/>
    </dbReference>
    <dbReference type="GeneID" id="123452136"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0076600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0076600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0076600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0093060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452136"/>
    <dbReference type="OrthoDB" id="2020426at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040244">
        <property type="entry name" value="EDR4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021480">
        <property type="entry name" value="Zinc_ribbon_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105">
        <property type="entry name" value="EXTRA-LARGE G-PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105:SF8">
        <property type="entry name" value="OS05G0586400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11331">
        <property type="entry name" value="Zn_ribbon_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable zinc-ribbon" evidence="2">
        <location>
            <begin position="240"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="780" mass="86039" checksum="1618C4B6D97E8678" modified="2022-05-25" version="1">MGSEPKEMKYRRRVRAPEPGDYGQCGDRGGGAVDWAALKQQDPAELLRKLDELRDQITRSCEAAGPPPHRMGRRAVPMRPSHADPLPPPPGRGAPPEYYRPSRHAGRYGPPSSYDQRSVCDDRYARQPSGRFRQPRPEGQWEGHGYGGQGSGHHSSCQCPQCVHGQRAVPPQEESIPMARFFAGQQRPPYQFDRSASSISSEYDRRSVASSLYSQHLSISKRRVEYFSKKADSFCRPAKGGAPFVVCSSCNQLLQLPPGKCTAPKQNQVQCGSCSEVVTFIKLKGVKVHPVIPSSSFSVPKSVRSSDRRAPQSSGWYPYQDDDTSSFDSYRQKQNFSDNLSQSSIGSYGSSTNKEHGPNKSSQVKPVSVSKSRFADSPKDILCQGDADSHVEASLIRTICPQAPVLEDKLVDPFSSQQNDCSGGDQIKSKRYGLNSKGNFDVRGERIDVKCEEKRNGKQKDGSGEETRKKTYGQKYLKQLNASCPTSHIGNKSMRKANSDDSSSLEDEVTSKKYEEKSKQDSNNFEVQGINKRYGNCDKEGYNNDLEFDSITKTCEEESIEDDYSKSVSSNCENAKTVAKNESSVSERTNTSSHVSSDAGLDEIQSSAGKSGDSSFFGGFLKKGFKDLSLFNQSMDSVKVSINGHPISERALRKAEKKAGPVSPGSYWYDQRAGFWGVMGHECSGIIPPFIKEFSYSMPKNCAGGNTGVLVNGRELHQKDFDLLVKRGLQRFSEKSYTVDISGNVIDAATGDKLRSLGKLAPTIEKMKRGFGMHVPEEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X260</accession>
    <name>A0A8I6X260_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123440067</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the type IV zinc-finger family. Class B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972592.1">
        <property type="nucleotide sequence ID" value="XM_045116657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X260"/>
    <dbReference type="SMR" id="A0A8I6X260"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277700"/>
    </dbReference>
    <dbReference type="GeneID" id="123440067"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0230070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0230070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0230070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440067"/>
    <dbReference type="OMA" id="DHYYKHC"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46813">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46813:SF15">
        <property type="entry name" value="GATA-TYPE ZINC FINGER TRANSCRIPTION FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="9">
        <location>
            <begin position="103"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="232" mass="24250" checksum="B8E84EED023D170B" modified="2022-05-25" version="1">MLHEAAPCTCGLLYGSCGGGCSMLFAAAPGDHHYYTKQCVDDGSYATYGGSVDCTLSLGTPSTRRAEAGVRAPAAGLPWEAVPSCNGRQDIGPARADQSNAGAASARRCANCDTTSTPLWRNGPRGPKSLCNACGIRYKKEERRAAAAAAAPTALASESGIEYAYGYARQQQQQQQQQWGCYGPAVAKAASYGMFGDAAAEDGPCLPWGLGVMPSSPAFGSVREMTSLFQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3I9</accession>
    <name>A0A8I6X3I9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3I9"/>
    <dbReference type="SMR" id="A0A8I6X3I9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0160970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160970"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26537" checksum="1D679642A9493AE3" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRYFNQSQYICTVRVFLDYPGGEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTTLTQLALVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3Y5</accession>
    <name>A0A8I6X3Y5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123430227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HIPP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970042.1">
        <property type="nucleotide sequence ID" value="XM_045114107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3Y5"/>
    <dbReference type="SMR" id="A0A8I6X3Y5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164110"/>
    </dbReference>
    <dbReference type="GeneID" id="123430227"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0135330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0135330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0135330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0164110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164110"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF367">
        <property type="entry name" value="HMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
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    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="86"/>
            <end position="149"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="209" mass="23280" checksum="CC8137BA15AFE116" modified="2022-05-25" version="1">MDLRVHPNHSNLASKRNATYPVIFPLTSSFLVYIYTHTRPSTVLYPRTLLLQSCEIKIMGVLDHLSDLCSMTDTKAALKLRKRRPLQTVNIKVKMDCEGCERRVKNAVKSIRGVTAVSVNPKMSKVTVTGFVEPSKVLARVKSTGKVAEMWPYVPYSLTTYPYVGGAYDKKAPAGFVRGAPQAMADPGAPEVRYMNMFNDEDVNSCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4M7</accession>
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        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="17202" checksum="7D858AD91EDCED2B" modified="2022-05-25" version="1">MSSLPSTRAASTAPRAARARSPQRPRPTDASTEAPHTRIPELSWPRRTRAELVQPRAVCAVSTDTDGCVMRACERLTTCVLLRSRAEPASPWVDAGIALGRRRRARRCGAHNLFGEMLGPLGRTNRDLLRCPYWHIRRACACHTYEQTSQQNGR</sequence>
</entry>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044974924.1">
        <property type="nucleotide sequence ID" value="XM_045118989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4P9"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234670.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="term" value="P:DNA-templated DNA replication"/>
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        <property type="project" value="EnsemblPlants"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="146"/>
            <end position="224"/>
        </location>
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        <location>
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            <end position="173"/>
        </location>
    </feature>
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        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234670.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="66785" checksum="EE6D3540DB4EF5AB" modified="2022-05-25" version="1">MVGPQYDLVGNPLGAVRATFERAAAAAAAESGGRDPVAAFRSKDWGASEVFRSFLFEQGGLDKVPVLDASNLGLIKPNTLVRFRGMVQDMLGNEFYAGAFKDGATWRTNKFTDFSPFSMPHPCDSHLWERHLFHCVPVPGQNSWTLDSSPGPDLRQMVNCLSPEQREKRKRDGDDDAMDVSENGNGESSSCSKKPKEDDVQIPSSSAEMPASENMPQMNGNDHHIPGSSFSCLVKIYDMPESQVKLNDVAEFIGVYTFDPELAAPNDNSDDIMYDLIEDVTAQLPPSKVPRLHCLVWRKLSSVDFLAKHPAVEPLPSLLKGIRQSLLSHLTLVLGKDDLAANCLLLHLLSRLRTRVDVVTVGRLSLNFTGFNRESVSIFGKQLNTLIQGLMPYSQAIPLSIEYLNTATLQPRKDNKSGRLVTAILQLPQGTHLTFDETLLQSGSLASKGVENTVLLKNLMESQMVEYDFEYYKLEMATDVQLLTLSEGKSNILPSDLVVPFRPSTVSEVNATSEELESWRWYLATVRSLPQSSEPEIYQTIQDEMVSAMRNDRSLGCTELSRWLTMAQITASSFGEKSLSLEHWQMVKELERLRKERMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X590</accession>
    <name>A0A8I6X590_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0166060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0166060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0166060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0166060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0166060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0166060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="21962" checksum="2992FC4B8D2F0FF6" modified="2022-05-25" version="1">MEFFTVILEKSSRLVLPHNFVKMLDGHRPQNLKLRQAGNGLRKLWDVEVVFDADGRMYLDRGWKQFVRANDLGIGYFLVFRYDGNATFVVKVFDTTMCRRRYQDDDDAGNGSRSSEYCNKCYVYGSSDSGSKSSSDSGDNKSSSDDGLVMDVPQLGDRAMPIVVEQYIPQPCLGLRRSKRIKMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFB3</accession>
    <name>A0A8I6XFB3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970290.1">
        <property type="nucleotide sequence ID" value="XM_045114355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFB3"/>
    <dbReference type="SMR" id="A0A8I6XFB3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0179320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0179320"/>
    </dbReference>
    <dbReference type="GeneID" id="123430486"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0148150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0148150.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0148150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0179320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0179320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0179320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430486"/>
    <dbReference type="OMA" id="DWMMEEH"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057441">
        <property type="entry name" value="Beta_prop_At2g24240"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF116">
        <property type="entry name" value="OSJNBA0029H02.24 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25279">
        <property type="entry name" value="Beta_prop_At2g24240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="9"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="451" mass="48852" checksum="264C3958FADB846D" modified="2022-05-25" version="1">MAARPGGGGRVRFNVGGQAFETTTTTLANAGRDSMLGALLDASWNVPCASDGGEDAAAEYFIDRNPACFAVLLDLLRTGSLHVPPHLPEKLLYREALYYGLLDHVRAARWGSFDGDRLRLASSVPGRAPGDGTAVRAAPDGGCCVAHGGAVHVYNWMLDERRPVSLDHSQVNDVAYLDAATLLIAAREHLGKCDGGMAVFSAVSGDLRHRFRVQHDRQAKSFTAGALAFDRDSRIFASCKGRLNEYGVGVWDSATGEQADFFYEPPGCALGDADRLQWLDATNALMVATLFPKTDNCSIGLLDFRDKSVAWSWSDAGTAASLEEKRVLHAIAMEDERSVCVINQYDDLGFLDLRSNAGAVRWSSRSKLMNRKAPGEESCYPKLATHGGQLFSSMNDSISVFSGPEHVLTSTLRRSYGGAICDFSIGGDRLFALHNEENVFDVWETPPPPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI54</accession>
    <name>A0A8I6XI54_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123445193</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978084.1">
        <property type="nucleotide sequence ID" value="XM_045122149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI54"/>
    <dbReference type="SMR" id="A0A8I6XI54"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309410"/>
    </dbReference>
    <dbReference type="GeneID" id="123445193"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0258310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0258310.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0258310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0309410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445193"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000002">
        <property type="entry name" value="p69E protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035190943" description="Subtilisin-like protease" evidence="12">
        <location>
            <begin position="24"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="15">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="13">
        <location>
            <begin position="138"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="378"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="16">
        <location>
            <begin position="668"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="785" mass="80913" checksum="CC6502586FD0777B" modified="2022-05-25" version="1">MPRRLLLLLLPLLLAARVRLGAGEDTGGERTYIVRVDADAKPSAFPTHAHWYESAVLAASGAGGGWPEGGPLIHTYSSALHGFSARMSPSAAAALAGAHGVAAVLPERVRRLATTRSPRFLGMLSSPPSAILADSDFGSDLVIAVIDTGISPAHRSFRDRGLGPVPPRWRGVCASGPGFPPGSCNRKLVGARFFSAGYEATSGRMNETAEVRSPLDNDGHGTHTASIAAGRYVFPASTLGYARGVASGMAPKARLAAYKVCWVGGCFDSDILAAFDAAVADGVDVVSLSVGGAVVPYYLDAIAIGAFGATEAGIVVSASAGNGGPGDLSVTNVAPWMTTVGAGSMDRAFPANVRLGNGQVLDGVSVYGGPVLQSGKMYELVYAGATSYSASTCLDGSLDQAAVRGKIVVCDRGVNSRAAKGDVVHRAGAAGMVLANGAFDGEGLVADCHVLPATAVGAASGEKLRKYIASSSPQKPATGTILFEGTHLGVHPAPVVAAFSARGPNPQSPETLKPDLIAPGLNILAAWPSGVGPAGIPSDGRRTEFNILSGTSMACPHISGLAALLKAAHPTWSPAAIKSALMTTAYTRDNSNGTMTDESTGKVAGVFDFGAGHVDPMRAMDPGLVYDIAPMDYVNFLCNLNYTEQNIRAITRRQADCRGARRAGHAGNLNYPSLSATFTADGAKAKMRTHFIRTVTNVGGGRSVYRATVRAPEGSTVTVRPERLAFRRDGQKLSFTVHVEAAAPMPPATAMEPGSSQVRSGALTWSDGRHAVVSPIVVTLQAPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI94</accession>
    <name>A0A8I6XI94_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Vacuolar protein sorting-associated protein 41 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123428377</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Required for vacuolar assembly and vacuolar traffic.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the VPS41 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968511.1">
        <property type="nucleotide sequence ID" value="XM_045112576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210930"/>
    </dbReference>
    <dbReference type="GeneID" id="123428377"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0175360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0175360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0175360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0210930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428377"/>
    <dbReference type="OMA" id="PQLVWQD"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16687">
        <property type="entry name" value="RING-H2_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000545">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 41 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000554">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 41 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016902">
        <property type="entry name" value="Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057779">
        <property type="entry name" value="Znf_RING_Vps41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23555">
        <property type="entry name" value="zf-RING_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028921">
        <property type="entry name" value="VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="12">
        <location>
            <begin position="41"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="9">
        <location>
            <begin position="611"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="Vps41 C-terminal RING finger" evidence="13">
        <location>
            <begin position="871"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="910"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="709"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="943" mass="105852" checksum="4E8E263F603D6EB8" modified="2022-05-25" version="1">MSSAARRAHHPSLVNGDGEYGPEDEEEEDEEEGEPEEEPRLKYQRLGGSVPAILSTDAAAAIAVTDRAVLLGTHDGTLHILDFQGNQSKEIKAHTATVNDISFEDREYIGSCSDDGTVVISNLFTDDKLKFEYHRPMKAIALDPQYSRSNNRFATGGLAGQVLVLTKSRWVSGYNKKVLREGEGPIHSMKWRTDLLAWANDAGVKVHDMKTDRGIAFIEKPKGIPRPEVLLPHLVWQDDTVLVIGWGTSVKIAAIRTDLSQGLNGMQRSVSAASSEKYVDIVGSFQTGYHISGIAPFGDLLVMLAYIPEEDERDKKNNTSVSSRQGTAQRPEIHLVSWKNDVLTTDALPIHGYEHYKAKDYTLAHAPFSGSSNAGGQWAAGDEPLYYIVSPKDIVVAKPRDTEDHIAWLLQHGWHEKALAAVEAGQGRTELLDEVGTRYLDHLIIERKYAEAAQLCPKLLRGSPSAWERWVFHFAHLRQLPVLIPYIPIENPQLSDTAYEVALVALTTNGTFHELLLTTIKSWPPTLYSASPVISAIEPQLNSSSMTNSLKEALAELYVINGQYEKGLFLFAELLKPEVFEFIEKHNLHDAIHDKVVNLMLLDSKRAVHLLIQHRDIIPPYEVVEQLLHASKSCDKKYLLHQYLHALFEVDIHAGKDYHDMQVELYADYEPRMLLPFLRTSQHYRLDKAYEIFAQKEFVKEQVFVLGRMGNAKEALSTIINKLEDIQEAVEFVTEQHDDELWDELIRQCLQKPEMVGMLLEHTVGNLDPLYIVSLVPDGLEIPKLRDRLVKIVTDYRTETSLRHGCNDILKADCVNLLVKYYHEARRGVCMASMDEEAQVARVNEGSSRTGDRSSSLRNLEIKSRTRCGARCCLCFDPLSIQDMSFIVFYCCHAYHQSCLEGGLDSMKSNSNARDSDEGSEDDEGSPSGESRMRCVLCTTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIF9</accession>
    <name>A0A8I6XIF9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123448784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044981725.1">
        <property type="nucleotide sequence ID" value="XM_045125790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIF9"/>
    <dbReference type="SMR" id="A0A8I6XIF9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380840"/>
    </dbReference>
    <dbReference type="GeneID" id="123448784"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0380840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448784"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000018">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF4">
        <property type="entry name" value="INACTIVE GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="153"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="334"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="622" mass="69898" checksum="5A719742C8F2E2EE" modified="2022-05-25" version="1">MVTTVLAVAASAAPAPPPSLAARFSPVAGPAVGFRAHCWITAKMKLHKALGRHGWQVQRKLDIRGDGKIPDYFEVAWRTGNITRRNVQPAGETGGETANTSSVMSDNSIDDKGLLHLGLNNSEADRPVLEEDLVLFDNFNDQPESVPSLCIAVIGATGELARSKVFPALFALYYSGFLPQNVAIFGYSRKTLADEDLRSMIESNLTCRVDHHENCEDKLNEFLKRTYYVDAGHDNKDGMAKLNLRMTQIEGTCAANRIFYLAVPQEALLDVALPLSDIAQTKHGWNRIIIEKPFGFTSLSSQRVTQSLLSRFEEKQIYRIDHLLGKDLIENLTVLRFSNLVFEPLWSRKYIRNVQVVFSEETSTETQGRYFGNYGIIRDIVHSHILQTIALFAMEPPVSLDGEDIRDEKVKVLRSIRKVDIDDVVLGQLKNTCGKVDQYTKSMTPTYLAAAMYIDNARWDGVPFLIKTGMGLMENRAEIRIQFHHVPGNIYRERFGHDIDLDTNELVLRDLPEEAILLKVNNKVPGLGLQLDASELNLLYRDRYDVEVPDSYEHLLLDVLDGDSHLFMRSDELAAAWSVLAPILHEIDQKRVAPELYEAGDKGPINAYYLAAKHGVRWDDGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIN1</accession>
    <name>A0A8I6XIN1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIN1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0361300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28969" checksum="A0717B7EC2186829" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVHDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQRAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGSLLPPVRGPRPQPCPYGSQGSQARVFPDPQVELPGLGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIR9</accession>
    <name>A0A8I6XIR9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123449932</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044983237.1">
        <property type="nucleotide sequence ID" value="XM_045127302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIR9"/>
    <dbReference type="SMR" id="A0A8I6XIR9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414210"/>
    </dbReference>
    <dbReference type="GeneID" id="123449932"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449932"/>
    <dbReference type="OMA" id="NYKGLRS"/>
    <dbReference type="OrthoDB" id="408728at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002338">
        <property type="entry name" value="Expressed protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="281"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="386"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="426"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="211"/>
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        <location>
            <begin position="344"/>
            <end position="364"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="757" mass="83394" checksum="FB5BA45E4676BA7C" modified="2022-05-25" version="1">MARVDARAVEEEFFDSREVVSPPSSVASPASSGRLDGEDGDGWLSAGALLEVWATGPCSSVEERRQRFVRSLGLLDPAHESQPRSWPGATEEEIVVLGNTGSADPASPAPRPSRGAGEEEAEEAGAPGGSGEEGLECVFKNLDDGTVFVVHELGKDGSFRSLRERRSNRTVTAAEFERISGSSPFIRELMRRVEDSSDEPSTPDKSAAARARRRRRRRRFGWLRRLGIGACVLDAEDEDEANSTSSSSCRSCSRKVLDRVKVRPHKKRSKELSAVYRGQEIKAHKGAIVAMRFSSDGQYLATGGEDGVVRVWRVVEGDRPDELDFAEDDPSCVFFTVNENSELAPVNSSEGTKNKQDKSSKGQADPACVVIPHRTFALSQVPVHEFYGHDDAILDLSWSKNGDLLSASMDKTARLWRVGCNSSLKVFFHNNYVTCVQFHPTSDNYFISGCIDGLVRIWDVRKCLVVDWANSKEIITAVCYRPDGKGVVVGTITGNCRYYDASENRLELESQVSLNGRKKSPLKRIVGFQYCPSDPKKLMVTSGDSQVRILDGVHVISNYKGLQSSSQVAASFTPDGDHIISASDDSRIYMWNHVNQLAPVTSRVKTVWSYERFFSNDVSIAIPWNASQSRNSISLACNIPSLRQEVSGDLCDIQDITSRCQTEDCLEDDNMFRLPSGNFTLSRAFLAESAPRGSATWPEEHLASNSATASTLRKSQYKFLKASCQNAATHAWGQVIVTASWDGHIRSFQNYGLPVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJM9</accession>
    <name>A0A8I6XJM9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lycopene beta-cyclase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJM9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268220.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268220"/>
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="LYCOPENE BETA/EPSILON CYCLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32098:SF5">
        <property type="entry name" value="LYCOPENE BETA_EPSILON CYCLASE PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321330.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="65495" checksum="399CFC8857DEA88F" modified="2022-05-25" version="1">MPPLPLPTAPLPPASCHGRRLALKPLRCRRGLLRVRASSETQPPPPPSRTQTIMDKISSAGDEVGGAGGAYSYDALKRLDQICSSICSAPQEASSSKAPPQIVTRVQGPARAAADLGAETFDVLVCGGTLGIFIATALSYRGLRVGIVERNAVKGREQEWNISRKELMEIVQVGILSEAEAEQVVTSDFNPNRCGFEGKGEIWVEDILHLGISPAKLVEIMKARFISSGGEIFEGKSVSSISVHDDLAVLKLSDGDRLRCRLVVDAMGNFSPIVRQIRSGRRPDGLCLVVGACARGFEKNTTSDVIFSSSSVKSVGGDSGRGVQLFWEAFPAGSGPTDRTTYMFTYVDPQFGSPKLEELLEMFWDLMPGYQDTVLENLDIRRVIYGIFPTYRDSPLPAAFDRILQVGDASGIQSPVSFGGFGSLTRHLGRLSNGVYEAVSGDFLDAQSLKLLNPYMPNLSASWLFQRAMSTRPGSDISPTFINELLFANFQSMQKLGDSVLRPFLQDVIQFGPLVKTLGLVMISRPQILPEIFKQVGVGVILNWSGHFVMLGYYTFLSSFVGPAVTPWVKSLAPRDRYQWDRYLEAWEYGAGLDYGQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMN2</accession>
    <name>A0A8I6XMN2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123439952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972500.1">
        <property type="nucleotide sequence ID" value="XM_045116565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMN2"/>
    <dbReference type="SMR" id="A0A8I6XMN2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270730"/>
    </dbReference>
    <dbReference type="GeneID" id="123439952"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0224380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0224380.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0224380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0270730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439952"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000646">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="305"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="507"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270730.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="65766" checksum="692B8CC2C0B3DAB0" modified="2022-05-25" version="1">MAPLPLPLPATPYPPKPHESPRPASLHAALASLSQQGSDHGSLRDAFALVSRAERQSSPGAAVAVGPEVYVSLLQCCVAAGSLRAGRQVHAAAVKRGPYYCRHAYIGTKLAVFYARCGALADAERVFDALPKKNAFAWAAVIGLWSRAGLHSRALDGYVDMLQAGVPVDNFVVPNVLKSCAGIGMVGTGRALHGYAWKAGFRECVYVLSSLVDFYGKCGKVDDAREVFDAMTETTVVTWNSMLMGYINNGRIDDAVELFYQMRVEGVLPTRVSLLSFLSASADFEAPDWGRQGHAMAVSGGLEMDVILGSSIINFYCKVGLVEAAEAVFEQMVERDAVTWNLMIAGYLQDGQTDKAFITCRKMLESGLRFDCVTLASIIMACMTSSSMEMGRVAHGYAVRNNLESDKAVACGLIELYMSSERTEHARGLFDVMSGRDMVMCKVMISAYADRGMSCQALEVLYQMQHEGISPTAACWDSVISAFMKNEQIDEALEIFSEMLLTKTRPNLRTWSLLISGLSRNGMHCEVMNLCCKMHEVEPAPSPTIFSAALVAMKAAASVQYGKAMHACIVKKGLLLSKSVIQSLLNMYGSFSDTGTVESLLGLLAAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUZ9</accession>
    <name>A0A8I6XUZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0322950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0322950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0322950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0269590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0269590.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0269590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0322950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0322950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0322950"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044246">
        <property type="entry name" value="ZFP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47287">
        <property type="entry name" value="C2H2 AND C2HC ZINC FINGERS SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47287:SF15">
        <property type="entry name" value="ZINC FINGER PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0322950.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="256" mass="26792" checksum="30300B574A90A95C" modified="2022-05-25" version="1">MGHSSRTASMEAVKAEDEEGEVVQPANEELSAPSLELNLVGALGLGENAAADAIVAAGPSDEKMAPAAVAGGENRRMFKCNYCQRKFYTSQALGGHQNAHKRERSIAKRVAAGRGGGPAASPYGAADHLVPHHLRYPGTWPYSAAGRPFLGRGAATAPFFGMQMQHHHMWNAAAQPSLAGLARYAGAHHNPMYRPEAYGYGASSMAPMSAFPVAAPAVSGIWRADASGSGNGGARSINESKKKEEAASKIDLTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XV86</accession>
    <name>A0A8I6XV86_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123441064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the abscisic acid and water stress-induced protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XV86"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0325150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0325150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0325150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0271270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0271270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0271270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0325150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0325150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0325150"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003496">
        <property type="entry name" value="ABA_WDS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33801">
        <property type="entry name" value="ABSCISIC STRESS-RIPENING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33801:SF8">
        <property type="entry name" value="OS01G0963600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02496">
        <property type="entry name" value="ABA_WDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0325150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="282" mass="29945" checksum="8CEC57274AB87580" modified="2022-05-25" version="1">MESSMSANPHRPSSPVTSTRLAINTPVLKTFGTSKQASTRTGVDHTLASLASPPALTMSGYYNGGGRTMSYSNTDECFDGGRTMYSNTTEECYDAGKHGHGHGHGHGHGHGGANMYSNTTDVECFESGRQGHGGGGRTMYSNTVDEECFDSGRHGLGHGHGHGYGHNDGRAMSYSTTTEECFGGGEQGQGYYKKEVKQHKNRERVGEVGAIAAGAFALYEGYEAKKDPAHARKHQIEAGVAGAAALGAGGYAYHEHREQKQAAAGYGGQQECRMPVHNSYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVB8</accession>
    <name>A0A8I6XVB8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVB8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0450770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0450770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0450770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0450770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0450770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0450770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0450770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26444" checksum="93930A31E5C1F925" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFQDYPGVEKPIHWSYGLGVTVDMAVQDAAYSMLSIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQTTVEMLENRDKDARALRMELYATRARLWTALTQLAPVVQIGYGEMEMLNPVRTHLPAHVDRPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XX36</accession>
    <name>A0A8I6XX36_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XX36"/>
    <dbReference type="SMR" id="A0A8I6XX36"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0466290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0466290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0466290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0466290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0466290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0466290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF99">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="594"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0466290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="684" mass="77757" checksum="5038404838ADBF71" modified="2022-05-25" version="1">MYIKIESCRLRWYRKNQTKIRADLYKGVVDAITSGETRASAVGVRIVLPGMYPSSNRDMKRRHMDAMSIVHTYGKPDIFLTMTCNPKWEEISNELLPGQTAQDRPDLVARVFYGKLEAMKELLFKKHILGVVVAYVYVVEFQKRGLPHAHFQLIMDASYKLVVPEQYDRVISAELPDKHKYPELYALVVKHMMHGPCGALNPNNVCMQQNKCKCRYPRAFNENTAQGKDSYPVYRHRDDGHRAKVRGKVLDNRWVVPYNPYLLRMFNCHINVEVCSSIKAVKYLYKYIYKGHDRASFSIDQPNANGIIDEIKRYVDARWVTPPEAMWRIFGFSLWGNSPSVLQLPLHLLNMHRVAFNEQADLTNVLASQSASKSMLTEYFKANQDHLWARHILYKDFPGSFTWQRTKYWKPRVERYQIGRIVSANPAEGERYHLRVILNHVAGKTSFEDMLTVDGILCGSFREAAERLGLIEADNTLDDCLTEAEQWAMPCSLRRLFATILVHCEPGDVRGLWDRNFGPMCDDYRRAHKCTNDVEQMVLLDIRGILQSMGKDIVDFALPCIDDEFDPTGGEAREVIEESTVEFDVNDTKLGSSLNLEQRVAYDKILAAVDRSDGGVFFVDGPGGTGKTFLYRALLAKVRSKGNIAIATATSGVAASIMPGGRTAHSRFKIPLSCDDGASCSFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYL1</accession>
    <name>A0A8I6XYL1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYL1"/>
    <dbReference type="SMR" id="A0A8I6XYL1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0437170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437170.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="73" mass="8252" checksum="35A26FD0002993EC" modified="2022-05-25" version="1">MLNRGTYREAIGVILPFELQEAFPLADILIYIVDKVVCNRKKQEIEVGCDLAGLVCFVVFSCNQFIGTEKRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZH9</accession>
    <name>A0A8I6XZH9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZH9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0361100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF87">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="158" mass="19026" checksum="5E9E07350E215904" modified="2022-05-25" version="1">MRCPLICNWAVEFHLPHRVMHQFGLFQPHPPEWVDTDTQLHRLDRRRQRKIKDWQKHHKNYVIMFEQSVQAATSMQGTQPREHCPLAFNNYVRWFQESTLVEICPSAYEEDILEEPTEYDALAHGEYNRLIREGYQTSFAPLLNFVRSRNKLMKPKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2I7</accession>
    <name>A0A8I6Y2I7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
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    </dbReference>
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    <keyword evidence="12 17" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="5 17" id="KW-0813">Transport</keyword>
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        <location>
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            <end position="93"/>
        </location>
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            <end position="365"/>
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            <end position="496"/>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0588790.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="504" mass="55337" checksum="C76AD7A3A808B42B" modified="2022-05-25" version="1">MATLRVDEIHKILRERIEQYNRKVGIENIGRVVQVGDGIARIIGLGEIMSGELVQFAEGTRGIALNLESKNVGIVLMGDGLMIQEGSFVKATGRIAQIPVSEAYLGRVVNALAKPIDGKGEIIASESRLIESPAPSIISRRSVYEPLQTGLIAIDSMIPIGRGQRELIIGDRQTGKTAVATDTILNQKGQGVICVYVAIGQRASSVAQVVTTFHEEGAMEYTILVAEMADSPATLQYLAPYTGAALAEYFMYRERHTLIIYDDLSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNSLLGEGSMTALPIVETQSGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVGSAAQIKAMKQVVGKSKLELAQFAELQAFAQFASALDKTSQNQLARGRRLRELLKQSQANPLPVEEQIATIYTGTRGYLDSLEIEQVNKFLDELRKHLKDTKPQFQEIISSSKTFTEQAEILLKEAIQEQLERFSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6K3</accession>
    <name>A0A8I6Y6K3_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="104" mass="12081" checksum="D29C0D94754C9270" modified="2022-05-25" version="1">MIQEVCEAEIASDPLVEKLNLVEKQNEYLKEKLKGIEEEKMKLELYVADVVDDHTIKTDAMCLKIRRIRNYALNKEAWYHYVVGSIVTLVVILITFVVAFKCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8J3</accession>
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            <fullName evidence="7">BRCT domain-containing protein</fullName>
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    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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            <strain evidence="9">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        </subcellularLocation>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.7HG0538690"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648920"/>
    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="52"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="196"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="330"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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            <begin position="148"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648920.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="38065" checksum="E2D7CC7068865C81" modified="2022-05-25" version="1">MPEPSSDPGNGKGKRSLPSWMGSGSGDGGGGPSPGKKKHAEAARERAPTGPDFSKLLDGVVFVLSGFVNPERGRLRSQALDMGAVYRPDWSDDCTLLVCAFANTPKFRQVQSENGTIVAKEWISESHSQRKLVDIEPYLMHAGNPWRTNKEPAESSQDQKKPRKEHEKQLEKTHAKTPPSVATKAGHSGAINKQFSPSKIKQWAMDDFAKTISWLESQEEKPEPNELKAIAAEGVITCLQDAIESLEQGNDISGVAEQWSFVPHVVDELVRLDGKGSSMSKEQLAQLATKCKKIYQAEFAQMDSDDKKGKERPGRTGVDDAQYDSDDTIEMTEEEIDLSLQTVLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9R3</accession>
    <name>A0A8I6Y9R3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123404041</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953887.1">
        <property type="nucleotide sequence ID" value="XM_045097952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9R3"/>
    <dbReference type="SMR" id="A0A8I6Y9R3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547600"/>
    </dbReference>
    <dbReference type="GeneID" id="123404041"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0455080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0455080.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0455080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0547600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404041"/>
    <dbReference type="OrthoDB" id="598311at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF55">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035254495" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="25"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="39"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="101"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="868" mass="95993" checksum="6DF709AB7053EB16" modified="2022-05-25" version="1">MHRSVAKLLALLLAMVIFSVAAHARQLQRAHAAGASCLPHERDALLALKQAINTDPYDNVLASWQRRHKHCCRWWGVTCSKETGHVIELDFGGTGLDGKISPSLLSLEHLEYLKLYATNLLGPDGTSLFPEFLCSLSNLRHLDLSYTQFSGRLPTQLANLSNLEYLDLSYTQFSGRLPAQLANLSNLEYLDLSYTSMSVILPPQLGNLSNLRHLGLGFMQDIHASDISWLTHFHFLEYLDMSDINLSSADVFLAANKIPSLKVLILINCSIPNANQSLTHVNLTKLEELDLSRNYLGHQIETCWFWNLTSLKKLGLDSTALYGPFPDALGGMISLQRLAFRNNGNSATMTVDLKNLCDLEFLVLDGSLAFANLTEFVRKLPQCASSKLWHLSSNHNNMSGMLPDSVGLFTGLEYLSLYNNSITGVIPTGLVNCSSLDIVILRLNQLSGQVPTLPRSLAEVDLSMNSLSGPLPSDFGAPDLSVLSLSSNYITGHIPRPICKLRHLYFLDLSQNRFVGEFPRCSSMSGLQFLYLSNNNFSGNFPALLQNCSELTFLDLAMNKFGGALPVWIGDLVNLRFLQLNHNMFNGDIPASITSLVLLQQFSLASNNISGSIPSSLSKLRAMTLKHLRRLDSRWSESSIILQLPVDILSVAMKQQELKFSGDAVINMVSIDFSLNQLTGEIPDEITSLNGLLSLNLSLNHLSNKIPVKIGDMKSLESLDLSRNNISGEIPTSLSDLTFLSSLDLSYNNLAGRIPAGSQLDTLYAEDPSMYDGNSRLCGRPLHRNCSGNSPPGYGNQQRGENAYDPVMFFYIGIASGFVVGLWLVFCAFLFKRAWRYAYFRLIDELCDKVYMFVVVTCGRINTKAIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YC81</accession>
    <name>A0A8I6YC81_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">sulfate adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123452179</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044984718.1">
        <property type="nucleotide sequence ID" value="XM_045128783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YC81"/>
    <dbReference type="SMR" id="A0A8I6YC81"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509820"/>
    </dbReference>
    <dbReference type="GeneID" id="123452179"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452179"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000002">
        <property type="entry name" value="ATP sulfurylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF65">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="14">
        <location>
            <begin position="55"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="13">
        <location>
            <begin position="231"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="473" mass="51929" checksum="021BDC8B8220CFFC" modified="2022-05-25" version="1">MATQAAFAVRFPQLARPSRGQGQPAAAAARVAVRGGGRAAAAAARGVRCRAGGLIEPDGGRLVELVAPEEGGRRAALRREAAALPHRVRLGRVDTEWLHVLSEGWASPLRGFMRETEFLQALHFNAVRGADGSLVNMSVPIVLALDDAQRRAIQASAATSVALVDAHDRPVAVLRDIEIYKHNKEERIARTWGTAARGLPYVEEAITNAGDWLIGGDLEVIEPIKYNDGLDQYRLSPAQLREEFARRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGFTKADDVPLSVRMKQHEKVLEEGVLNPESTVVAIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDTKHNKMNFFDPSRKEDFLFISGTKMRSLAKNRESPPDGFMCPGGWKVLVEYYDSLAPPEGSSKLREAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YG09</accession>
    <name>A0A8I6YG09_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone chaperone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123448434</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044981244.1">
        <property type="nucleotide sequence ID" value="XM_045125309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YG09"/>
    <dbReference type="SMR" id="A0A8I6YG09"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0364440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0364440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0364440"/>
    </dbReference>
    <dbReference type="GeneID" id="123448434"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0303540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0303540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0303540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0364440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0364440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0364440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448434"/>
    <dbReference type="OrthoDB" id="514832at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037647">
        <property type="entry name" value="HIRIP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410:SF2">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="5">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="219"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="385"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="441"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0364440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="480" mass="52795" checksum="0FA7792310EA54C6" modified="2022-05-25" version="1">MLLPPKEVLSATSDHHYHRRMEADGAAAAAATRGREAEIIEALRARVPFFKKQADSLTLEGVRRTLEKDMDLKINSLDPHKKFIKQCVDKVFSGCDNESTDNASERAEAKVDNLSEDAQPMFGSNTISSSSDEQGARSTETNKDPEGAKNHSSGIDITEAMIMKAIDKRASYFRENSETLTLLGVRRTLEEDLKLEKKALDAFKGYITKELDRVLQEPENGMADHSKQEACKDAGQKTSKGSKRARQDSDTFELNNSHSEREDSDEDIRPKKRGPKKGKSIKQQKKVTAGKKLSTLKVEKVAKRDLDKSDKDQGRNSAEDNPLSSAVDNKKAAPAHGKRVERLKSVIKSCGMSVPPSVYRRAKQAPESKREACLIKELEHILEKEGLSSHPSEKEIKAARKRKERAKDLEGIDMSNIITSSRRRSASSFIPLAVPKIEAGSDSDDDDDGEDENVEGGDKGDDDEAEAGDGSADDAGNGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIH8</accession>
    <name>A0A8I6YIH8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase I-specific transcription initiation factor RRN3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123413502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962371.1">
        <property type="nucleotide sequence ID" value="XM_045106436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIH8"/>
    <dbReference type="SMR" id="A0A8I6YIH8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730720"/>
    </dbReference>
    <dbReference type="GeneID" id="123413502"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0606320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0606320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0606320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0730720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413502"/>
    <dbReference type="OrthoDB" id="26970at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="72073" checksum="A0856740808B995D" modified="2022-05-25" version="1">MVAELRRDDMAGEAMPVRDAVAFRLVEHVLQSVRMEPLLVNPYDQEQYGKMLDTLDTSKRKSADDEALYVTYLKALSEAVSKIDIMYHHSLLNNIFSVRIWYLQRDTMVALLDLITRLAAVADQYLRECLQMLVNNFTPPIIVKHNNEVPPWAVSRKKEIFSRLCESLKTISDTVPLAPRMLRDIIDRSMPKLFDNKAKMISFVECMLGLDTDRMGDLIGAILLAKVVDLITELDVNITWEDILQEEHNKGIFEMELEDLDGDEDGDGFGQAGTKVLFGGNACAEKLDGLMVVVCEHLKSLDRQCLYKEFVILKTIFRASLLRVHRSKFAQFIMFYACSLDPLILGEEFALFLTDIVKNKEEDPISRMSAVSYVGSYLSRARFIPANTVVTILKKLVDWCVGYCDYQSGRDATAIPNHQLFYATCQAVMYVLCFRLRSIMDYPNLKSDLFHMPFGFLFRHRLEPLKVCLPSIVNEFLRQAKDAGLVAAFVDPASEDAIESDLSRTFGGIDRLDMFFPFDPYLLKESDRYIRPNFEFWSMVKTTYSDDTDDDDDELEGLDAPEMNVGSLDDHVEIDINSDDDDLEYSMNKMSITPHHSFFHQMAMDSDGGLSMPARIRPSTSPPSRWAMSGGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YL14</accession>
    <name>A0A8I6YL14_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956661.1">
        <property type="nucleotide sequence ID" value="XM_045100726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YL14"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0725150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0725150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0725150"/>
    </dbReference>
    <dbReference type="GeneID" id="123407564"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0601390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0601390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0601390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0725150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0725150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0725150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407564"/>
    <dbReference type="OrthoDB" id="552995at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34684:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34684">
        <property type="entry name" value="OS08G0192200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0725150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="29375" checksum="963EB37188EB19A3" modified="2022-05-25" version="1">MDLGTENRLAALLLEEARRLRMQAEKEGVHAYLQKPNVRHRPNSRFLAATVLGVQHANRVVEVNEMWRAREKELELESKMKSRTNGLNDWRSQKRKNDSRDQSPVSKIQRDRPYGASFSSRDSHSSSYSDQEDGLGDDEIEEFLRSRVKRGLGAIGSRMDEPGPYLKPPSSRQDNSANLDAREKEERKRRVYGPEKPLFLRSMKSSDEEPSTLKHKHIKEEKKNSSNRERKEEENRRAKHHHREHKSRSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YN20</accession>
    <name>A0A8I6YN20_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YN20"/>
    <dbReference type="SMR" id="A0A8I6YN20"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0699080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0699080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0699080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0699080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0699080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0699080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052160">
        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0699080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="130" mass="14918" checksum="F445F888D35BB690" modified="2022-05-25" version="1">MPMNYSLVIEPFDVWGFDYMGPFPSSNGYTHILVVVDYVTKWVEAIPTKNADHHTAIKMLKEVIFPRFGVPRYLMTDGGSHFIHGVLRKMLAKYDVNHRVASPYHPQSSGQVELSNREIKLILQKTVNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YN32</accession>
    <name>A0A8I6YN32_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YN32"/>
    <dbReference type="SMR" id="A0A8I6YN32"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0622100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0622100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0622100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0516080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0516080.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0516080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0622100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0622100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0622100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0622100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="102" mass="11686" checksum="FFFF08AA65750B05" modified="2022-05-25" version="1">MTLPNTFMSADQIFEFVRDADCYLNVSIAYRILLTVPVTVATAERSFSKLKLLKNYLRSTMSQERLNGLSMCCIEKNMLDSINLDTIIDDFASKYARKSHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNS2</accession>
    <name>A0A8I6YNS2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vesicle-associated membrane protein 727</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123407871</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045101107.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957043.1">
        <property type="nucleotide sequence ID" value="XM_045101108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNS2"/>
    <dbReference type="SMR" id="A0A8I6YNS2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0689340"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689340.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689340.2"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0689340.1">
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689340.2"/>
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        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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        <property type="term" value="P:protein targeting to vacuole"/>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vesicle-associated membrane protein 727"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vesicle-associated membrane protein 727"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
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        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
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        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF169">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="9"/>
            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="153"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689340.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27712" checksum="3C907E51F00D4FEB" modified="2022-05-25" version="1">MNGGSKQTLIYSFVAKGSVVLAEHTAFSGNFSTIAVQCLQKLPSNSTRSTYSCDGHTFNFLVDRGFVFLVVAEEALGRSVPFVFLERVREDFMQRYGSSIDEEGQHPLADDADEDDFLFEDRFSIAYNLDREFGPRLKDHMQYCINHPEEINKLSKVKSHLSEVKGIMMDNIEKILDRGEKIELLVGKTETLQSQADSFHRHGRELRRKMWLQNLRFKLMVGGGIAFLILILWLMVCRGFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQ49</accession>
    <name>A0A8I6YQ49_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        <location>
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            <end position="177"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720610.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20347" checksum="3D27DFBE7064FDCD" modified="2022-05-25" version="1">MAPKKTPKGKSGFFGVRQKPSGHQDEGDPHEVVRAYDKAVWHAERPREHLNFLEIESRVEAEMLVPQGIKMKEITTKKTATKKPSVVINAGETDEEAIARFAREHPEYVQAELEHYWKREAEQKKKEDEAGPSTVIPIESSSEEDWEDFSTAAEEEGCDDPEKEDFWEQFRSSDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQJ1</accession>
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            <fullName evidence="6">Reticulon-like protein</fullName>
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        <name evidence="9" type="primary">LOC123410765</name>
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    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
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    <reference evidence="9" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044959641.1">
        <property type="nucleotide sequence ID" value="XM_045103706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQJ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712170"/>
    </dbReference>
    <dbReference type="GeneID" id="123410765"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044647">
        <property type="entry name" value="RTNLB17/18/21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46626:SF4">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46626">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="190"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="411" mass="45362" checksum="7A720B232EB0BAC5" modified="2022-05-25" version="1">MEIEIEPDSPRRLTPAPMPRSGDIAVADGTATLSPPPSRPQGRASPSPPLSPRDDVLPLSPPRDSPARRRGRSKPLRTTVVDQGAGAVGSPRRKRRGAGEPRAAASPGKNVRRARRRLDSDGGRELTANEAADEEAAGKTQRRKTAAQPRAAVKEKKGSSLALVPYPPINPTRGAENVEQFDWEGLWKRVVDLMMWKNVGRSAFWFGSGSMLFLSSSLSRDVNFSPIKILCHFGVVTLVLAFFKDSIPRRQNTEQVKSFQLTEEDVLRIGRAVLPIANSLISMSRVIFSGDPSMTLKVLPILLFGAKYGHLLTVWRLLATGFFGCFTLPRLYSCYSSHIHEKVEGLNARILNAWKSCPRKKLVAAAAVTTFWNLVSVKTRVMTAFVSAVALRYHYHYGRSSRNSEGAIRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YW21</accession>
    <name>A0A8I6YW21_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YW21"/>
    <dbReference type="SMR" id="A0A8I6YW21"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF295">
        <property type="entry name" value="OS10G0429651 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="197"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="388" mass="43729" checksum="2F0C8CE0C216F588" modified="2022-05-25" version="1">MSSFAGVTVLDGNNRCHCEMSGVHASTDVDTGYHLLIIKACSRINGLVPTGQNITCGPFVIGGHEWVLDYYPNGENPSCADYISVYLTLLYDADVDREEPVEAKFSFSLINQVEKQMPVYIRGTKTYSFSSSAPMWGISKFVRRDVLEQSMDMACDCFTIRCDIMVCEDPKTKDDAGGTLPDIHQHFSNLLDNKLGADVTFEVGGETFAAHRAQLFGPMKEGTATSSIIQIKDMEAEVFRALLSFVYTDSFPEMYKDIMEENRMIVSEEQEQADEAVDDEMSETREMELQWLHDLLVAADRYDLQRLKFICEKRISERIGVSSVASSLALAEKHRCRGLKEACLEFIQVQSPSRLKRVMATSGWEHIMMTYPSVLNEFIAKLISSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZA6</accession>
    <name>A0A8I6YZA6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123402050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044951845.1">
        <property type="nucleotide sequence ID" value="XM_045095910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZA6"/>
    <dbReference type="SMR" id="A0A8I6YZA6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551730"/>
    </dbReference>
    <dbReference type="GeneID" id="123402050"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0458430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0458430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0458430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0551730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402050"/>
    <dbReference type="OrthoDB" id="600272at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000001">
        <property type="entry name" value="probable alkaline/neutral invertase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF42">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="569" mass="65027" checksum="61892CF9F6C083A1" modified="2022-05-25" version="1">MVLSPIKAARSKVTELTDDTKYDSLNLEQKKKMWPIERHRSAEVSQAFLSKIEHDGFQLCHQPQILEIVKGGCTPRSSSDPPGESTADSNGMHRHTITDAAWEALKQSIVYFKDQPIGTLAAIDKSQAELNYDQVFMRDFVPSALAFLMKGEPMIVKNFLVETARLQSREKMVDLFKLGQGVMPASFKVHHSHPTKKTETLLADFGEIAIGRVAPVDSGLWWIFLLRAYTKYTRDSSLAESPHCQRAMRLILKLWLSEGFDTSPALLCADGCSMIDRRMGIYGYPLEIQALFFMALRCALSLLKDSNDDFVYQITKRIKALSYHLHSYYWLDFQRLNDIYRYKTEEYSQTALNKFNVIPESIPDWIFDFMPSRGGYFIGNVSPARMDFRWFCLGNFIAILSSLATGEQAEAILDLVEERWEELIGEMPLKICYPAMENQEWQIVTGCDPKNTRWSYHNGGSWPVLLWLLVAVSVKLGRPHIARNAVELMEKRLAKDDFPEYYDGKTGRYIGKQSRKFQTWSVAGYLVAKMLLDDPSNLRAVSLEDDGHIREPVLKRSNSCPGPHVSNWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z0U9</accession>
    <name>A0A8I6Z0U9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0U9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0574010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0574010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0475880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0475880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0475880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0574010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0574010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0574010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006734">
        <property type="entry name" value="PLATZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065:SF51">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04640">
        <property type="entry name" value="PLATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="215" mass="24081" checksum="7B5407EC86483BAD" modified="2022-05-25" version="1">MGMPFDHQAASWGRRTPAWLKPLLGAEFFKPCADHPYLVKNECNHYCLDCAGEDDAVCCTLCVSAHRNHRVVQIRKSSYREVIRVAELKAVADVSLVQTYVINYDRVVFLNRRPQAPQHGVKCVGPAGACRECGRGLVDANFRFCSLGCKLDGMVFDANLTFMVHPRCHRENSGSNTEEEGEEDGDPSGPSDPPPGWISYRRRPRKGVPERAPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z5Q7</accession>
    <name>A0A8I6Z5Q7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123409820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958628.1">
        <property type="nucleotide sequence ID" value="XM_045102693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5Q7"/>
    <dbReference type="SMR" id="A0A8I6Z5Q7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636690"/>
    </dbReference>
    <dbReference type="GeneID" id="123409820"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0636690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409820"/>
    <dbReference type="OrthoDB" id="695235at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF1">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035228509" description="Peptidase A1 domain-containing protein" evidence="7">
        <location>
            <begin position="37"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="87"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="456" mass="46982" checksum="084AF9364AF3BAF8" modified="2022-05-25" version="1">MARPAASTDGSALAVTLVGWLLVLQLLLLAPAPTAARRATSVNQTPNFLSRKPPRSGTNALLKKARRRNRNTDLLGSAAADDAGYVVLYNVSIGATPNDVSGVVDVLNDFVWTTQCVAAPVRVQCASQTCRSLLANDTMDACGGNPSGDDTCSYVNVYAPGSNTTGFLANETVAVGSFVGAAILGCSAANSTGPLVGEVGSFGFNRGALSLVSQLSVSKFSYYLAPDEAGSSDSESVVLLGDAAVPQTRGGGRSTLLLRSTAFPDVYYVKLSSIQVDGQALSGIPTGAFDLAADGSSGGVVMGTLSPVTRLQVDAYNALRQALVSKINAQEVNGSAFAGGVFDLCYDAQSVATLTFPKITLVFDGGNAPATLELTTVHYFFKDNVTGLQCFTMLPMPVGTPFGSVLGSMVQAGTNMIYDVGGETLTLEEGAAAPPPSQVVSLMAIASLLLAWVLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z6X9</accession>
    <name>A0A8I6Z6X9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z6X9"/>
    <dbReference type="SMR" id="A0A8I6Z6X9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650530.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650530.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0539850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0539850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0539850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650530.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650530.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <location>
            <begin position="162"/>
            <end position="264"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
    <sequence length="312" mass="35188" checksum="9224E9669328A4A9" modified="2022-05-25" version="1">MEYSQKFKILDIPPVVQELVAGGVHEPPVQYKVPEQDRPAAVSEMPEPIPVIDLGRLSANNAEEFAKLQSALENWGFFLAVGHGMEPSFLAETMSVSKEFFKLPLEEKQKVSKIAYGDTLSIEGDILSEYTVKCRAVANIVLQNMAKLLNLDEEYFTNKFADTSYTLVGFNYYPPCPKPDHVFGLRPHTDGSAITVNFIDADVSGLQFEKNSTWYNVPIVPTALVVNIGDVMEILSNGFFKSLMHRVVTNTEKERLSLAMFYSLDMEMDIEPVPDLLDDKRPPRYKIKNKDYIAKQTYIFATGKQTIDTLKI</sequence>
</entry>
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        <lineage>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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    <reference evidence="2" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </reference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0709370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28973" checksum="33A86E16DABF2916" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTIEMLENRDKDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRNHLPAHVDWPAIGGVTPLRGPLLPPIRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0R0</accession>
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        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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        <name evidence="20" type="primary">LOC123427868</name>
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    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
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        <property type="nucleotide sequence ID" value="XM_045112007.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I7B0R0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0032260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0032260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0032260"/>
    </dbReference>
    <dbReference type="GeneID" id="123427868"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0025370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0025370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0025370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0032260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0032260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0032260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427868"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000239">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000207">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF35">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="283"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="168"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="610"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="610"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0032260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="657" mass="69321" checksum="88377A12F2440152" modified="2022-05-25" version="1">MSSPTAAPAPTSPPAPPANATAPPPATPSAPPPATPSPSPPAPANPPPASVPPPAAPSASPPAPSSTPATPSAPSPSPPGSPAAPSPPSDTPSPPSPEGRSPPSSGGGGKSPSTPGHNNPSPKSPPHSSGGGGSGVSTSVVVGVAVGGFVLLLLATFVCLCCLRKKRRRQPPPPHYGYPPPPPQQYKDDTYGATYQNWHNNVPPPPPDHVVKMHPSPPPAYANRPPQAPPPPPPPMINSSGGSGSNYSGGEILPPPSPGTALGFSNSKSTFTYDELVRATDGFSDANLLGQGGFGYVHKGVLPNGKEIAVKQLKLGSGQGEREFQAEVEIISRVHHKHLVSLVGYCISGGKRLLVYEFVTNNTLEFHLHGKGRPTLEWPIRLRIALGAAKGLAYIHEDCHPKIIHRDIKSSNILLDFKFEAKVADFGLAKFTSDNNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGRRPVDSTQTYMDDSLVDWARPLLMRALEDGNYDELVDARLGKDFNPNEIARMIACAAACVRHSARRRPRMSQVVRALEGDVSLEDLNEGVRPGHSRFFGSYSSSDYDSGQYNEDMKKFKKMAFTTNDYTSSQYSAPTSEYGQIPSASSSEGQQTQEIETGTMKKGGHSGYSSGYSGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2G9</accession>
    <name>A0A8I7B2G9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B2G9"/>
    <dbReference type="SMR" id="A0A8I7B2G9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0013910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0013910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0013910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0017920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017920"/>
    </dbReference>
    <dbReference type="OMA" id="CQHGRDE"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9744" checksum="F549408B9D38F19C" modified="2022-05-25" version="1">MKVRDYELDQYGVVNNAIYASYCQHGRDELLEGVGISADAVARSSESLALSEMHLKYFTPLRSGDKFVVKVRSDTKTPKSSILLSGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3D0</accession>
    <name>A0A8I7B3D0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPR1-interactor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3D0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0060200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0060200.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0060200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0047780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0047780.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0047780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0060200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0060200.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0060200"/>
    </dbReference>
    <dbReference type="OMA" id="EPPLWKP"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010112">
        <property type="term" value="P:regulation of systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031425">
        <property type="entry name" value="NPR1/NH1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33669:SF22">
        <property type="entry name" value="NRR REPRESSOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33669">
        <property type="entry name" value="PROTEIN NEGATIVE REGULATOR OF RESISTANCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15699">
        <property type="entry name" value="NPR1_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0060200.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="162" mass="17276" checksum="681FCE6735315A8B" modified="2022-05-25" version="1">MDATAADRQEAPPTASDGAPTTSVMEVPELSELPIAGDGGTPEESGGGEDEQVERFYALLANIRALRDVYGYGAGGSSRKRARGAEPPLWKPKFRMEDFREADDSLPGKKGKSDRVERKRLENSDAEEADGEDGEVVQENDRASASQTTRVLAAPACADCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B512</accession>
    <name>A0A8I7B512_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkaline phytoceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975326.1">
        <property type="nucleotide sequence ID" value="XM_045119391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B512"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244600"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34368">
        <property type="entry name" value="OS01G0962200 PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
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        <location>
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            <end position="157"/>
        </location>
    </feature>
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        <location>
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            <end position="186"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="30960" checksum="1F8CCC1ED5292867" modified="2022-05-25" version="1">MHLAADYSHRGGGRLGLGPFAAAVLRTDNRRRAIAGGAVLASALLLVATPRLRHSPALHLFADMRNLLGVPNTLNVLTAYPLLLAGVPGLILCLFGSGCFGISLRWEALGWFLFYAGNVGAAFGSAYYHLKPDDDRLIWDRLPMMMSASSLLSILVIERVDERAGLSCLISLLSLLLVSSACERILDDMRLWVVLNLVPCVAIPAMLFLFPPKYTHSRFWFLATGFYLLARFEGLADRKVYSVNRYFISGHSLEHLCFAMVTLILTVMLSFRNIKIARDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Inhibits both subtilisin and chymotrypsin.</text>
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    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5A5"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122530.2.CDS2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="68" mass="7668" checksum="6B3B72F0E3145CF9" modified="2022-05-25" version="1">MSKPCDGPKTEWPELVGRTIEEAREKIKADRPDLTVNPVPVGDAVPLDFNEKRVWLWVDTVAEVPRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5K7</accession>
    <name>A0A8I7B5K7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123429849</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969760.1">
        <property type="nucleotide sequence ID" value="XM_045113825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5K7"/>
    <dbReference type="SMR" id="A0A8I7B5K7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134600.1.CDS1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123429849"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0110900.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0110900"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429849"/>
    <dbReference type="OrthoDB" id="660799at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000012">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
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    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="11">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="11778" checksum="E2314B0ACA27A787" modified="2022-05-25" version="1">MSGRGKGAKGLGKGGFKRHRKILRENIYGITKPAIRRLARRGGVKRISGIVYEETRVVLKLFLEHVIRDVVVYTQHARRKTVIATDVVYALKRQGRTLYGFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5R8</accession>
    <name>A0A8I7B5R8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein FLX-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123425942</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965643.1">
        <property type="nucleotide sequence ID" value="XM_045109708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5R8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142490"/>
    </dbReference>
    <dbReference type="GeneID" id="123425942"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0117350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0117350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0117350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0142490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425942"/>
    <dbReference type="OrthoDB" id="1928946at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040353">
        <property type="entry name" value="FLX/FLX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405:SF7">
        <property type="entry name" value="PROTEIN FLX-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="155"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="37974" checksum="739032EFD6B4EA68" modified="2022-05-25" version="1">MAGRHRNPLPSSFSRGAGGGNHPHPPPPHHAHLPPYHLDEFREPPRLPPHHLDDFRDPARLPPGHPDSLREHPPPPRHHFAGHGGGTLPPASHMAAALEERIGAEIEEAHALLGQNQRLSATHVALVQEVSAVRHELGHTARAIGAAQQEGDLRIREVYERLMKMEAELRAVEEMRAELAHVRMDIQQLGAARQELMGQIQGYTQDLARSAVELQQVAAVKAETQELRHETQHLRSSIELEKKGYAESYEQGQEMQKKLVSVASEVEKLRAEVANAEKRSRVAVSAGNQGYAGSYGNPNANYAANPYNVGYSMNQVNATDSGSQYGAGGAHSSWGAYDMQRAPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6A5</accession>
    <name>A0A8I7B6A5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123445249</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978146.1">
        <property type="nucleotide sequence ID" value="XM_045122211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6A5"/>
    <dbReference type="SMR" id="A0A8I7B6A5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310710"/>
    </dbReference>
    <dbReference type="GeneID" id="123445249"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0259500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0259500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0259500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0310710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445249"/>
    <dbReference type="OrthoDB" id="657274at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF94">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="4">
        <location>
            <begin position="335"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="653" mass="73666" checksum="D5221E2EFA5A589D" modified="2022-05-25" version="1">MHLTTVSRSASEKVHLAVGDDPKQWWLTLIWASRCFIPQHNLLVLVHIHRPAIPTVLGAPVPASTLTEESICVHMTNMRKIIENNFKKCLQLYEVHAEILIIDNDKDVGVALVEVIKEHKITTLVMGAKRRKHGWKSRRAVALEKQADCLCNILYLHNGFVVDSRHQHTDRKIGVPSLRGCHFSGSSSNATKSSSFFNSCSTADAFDVEHLDDPSLVMNTTYIYNDSRFNAIIGLKSFDSFRELVNQQILAEHSRDLYQAFHIKYCDIFTGCQFIGGFDSLIGVDSQNLGDAHWKSIKSWPAVLDYIVSVLKTLKMQLLMQNHRACNGFTHDDLSEAVKIPIRRLFAVASMVSDLEVRKSPEKLFCVLNMYTALVDAAPTLRKVFSTKSIRDVLGELEDSVRGILKELKGLIHTYNSPKVVLDGDILPISGYLMRYIVLLVKHKVSLDTILGHDYTDSLLKVEGMSLTGGLVSGLITDLESVIDKQSKQCAASKGLRCIFLMNNIHFILQEVKQSDVQLTVKAKWFEKRQSCIKGYIKSYLSALWVPVTSTLEVARSVPPSKRARTNLLNFWYTAPSLTPLQSFASAFNEACSCQICWKVPSPALRDELRVKILEFVSRAYHGHLESQRQSAEQNANDFELEWKRKISELFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B926</accession>
    <name>A0A8I7B926_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B926"/>
    <dbReference type="SMR" id="A0A8I7B926"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0190550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0190550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0190550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229750"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="361"/>
            <end position="421"/>
        </location>
    </feature>
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        <location>
            <begin position="440"/>
            <end position="505"/>
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            <begin position="521"/>
            <end position="553"/>
        </location>
    </feature>
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        <location>
            <begin position="659"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="471"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="745" mass="84728" checksum="446402E83A04E1FF" modified="2022-05-25" version="1">MNGRMIQSVMGSGDKSSSADFHGIMANTAGCGAYCSQDFIESSTAKYNDSELFSYPPIPVSCYPPLSIVSNKVGHVPWSTSSSYKVYSPQLFSVSCDETSAGWRLISVAEHQKTDSDITLSHIEQMLMQDTDDKVRPPHGETAIRTMEEPFYELLGQKYPVLPELLPLCACHRLKNLNGSVSRITGQLCSNCSVNVSSHNYHSNVDSQAFQAPWTLSAIVGEAKQFPLGAERMEIGLNISGLSIAKKPGRDNQSLHMIDKDTIKDASFEVRGRKVFRGTEELDLLEGRRNKQIAIFPDEPIRNEAFDKVLLCSEHNKPVDEDIILQQDMADKSTKYPQKDQGRKPARRMTRGKTRKKKEVVDLRSLLIHCAEAVSVNNRTLASDILKSIRQHCSPSGDDTQRLAFYLADCLEIRLSGSGSQINQKVITTPTNAAHILKLFHLCFTVCPYLRSSYYLSNKTILDVSKGKPRVHIIDFGIFYGFQWPSLLKQFADREGGPPKLRITGIELPMPGFRPDEMNKNTRLRLTEYANIFQVPLEYRQISSKWESISIEELNIDKDEVLVVNCILRMKNVGDETMSINSARNRVLNTIRMMKPEVFIHGVVNGSYNTPFFLSRFKEVMYHYTSMFDMLEKTTPRDNETRTVLERDIYKYAILNAIACEGSERIERPESYKKWNVRSIRARFEQLPLNPTIVKGIQYMVRQIYHKDFFVDEEDQCLVLGWKGRILYALSTWKPSESVDEGDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDF1</accession>
    <name>A0A8I7BDF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123447526</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980068.1">
        <property type="nucleotide sequence ID" value="XM_045124133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDF1"/>
    <dbReference type="SMR" id="A0A8I7BDF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416070.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123447526"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0346770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0346770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416070.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123447526"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF120">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="252"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="357" mass="37072" checksum="204C95D67BC67B16" modified="2022-05-25" version="1">MAPEWEAALGMELGMGTHYHHAPPSAAASPMNHHHHHHHGYSHSQPPHQYHFYGGAAGGDGADPMRVDEMLDLSSHLGAHDFFSGAANGQGGDNAPAPPAAAAASSSDHQHHHPSSFNLSFADEFYLPVPTEEAAELEWLSNFVDDSYPDVPNYPPAVQAAMAAAARNGGVVVKQENSASAAAPGRGARSKRSRAASAAAAAWHALAPRQPSPSSSSSSSDSKPARSGGGGGGVKKSGLVVGAAELGGGEQSGEVRRCTHCASEKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTQHSNSHRKVMELRRQNELVHLRGGVAGGVVSGSSGSGGAAEHMFRDYGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFQ9</accession>
    <name>A0A8I7BFQ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFQ9"/>
    <dbReference type="SMR" id="A0A8I7BFQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0436570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0436570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0436570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.310.10:FF:000001">
        <property type="entry name" value="Serpin family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.310.10">
        <property type="entry name" value="Serpins superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF325">
        <property type="entry name" value="SERPIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="309" mass="33562" checksum="388BE4B22D38A640" modified="2022-05-25" version="1">MVAAGTRGDTLSELLGFLGSKTIDQLHRAKATELVGRLNGLAQTFFASGVWARQHVNAFVADATDNLIRDVLPPASVDSSTAVVLANALYFKGAWYHPFDVSTAPFHVPGGTTVRVPSMTTSESQRIAVYPGFRALKLPYKNDVQVQQQEEFYMLILVPDKDTVSLADLYDKAVSTPEFFKKHTPAREVPVGRFMVPKFSFTFEFEASSAMRKLGVTKAFEDGDFSGMLGGGDKGLVIDKVHHKATVEVDEVGTVAAAATVVEIKYGSVGPGLRRGVYFVADRPFLFAVVEERTDALLFLGHVVNPLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGG2</accession>
    <name>A0A8I7BGG2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401285</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SFH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950993.1">
        <property type="nucleotide sequence ID" value="XM_045095058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGG2"/>
    <dbReference type="SMR" id="A0A8I7BGG2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554700"/>
    </dbReference>
    <dbReference type="GeneID" id="123401285"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0460800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0460800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0460800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0554700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401285"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL_PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SFH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="7">
        <location>
            <begin position="156"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="632" mass="71840" checksum="0670347B65A95F5B" modified="2022-05-25" version="1">MSGHLDRLVRPVSGFEGCSSHHDERRSYERRSDFEISEDEKKTRMGSLKKKAIDASSKLRHSLKKKNRRKSGSRVLSVSIEDVRDLGELEEVEAFRQALILDELLPTRHDDYHMLLRFLKARKFDIEKAKQMWTDMLQWRKEYGTDTIVEDFDYTELDTVLEYYPHGYHGVDKEGRPVYIERLGKVDPNKLMNVTTMDRYVRYHVKEFERSFLIKFPACSLAAKRHINSSTTILDVQGVGLKNFSKTARELIMRLQKVDNDNYPETLHQMFIVNAGPGFRMLWSTVKSFLDPKTTSKIHVLGNKCQSKLLEIIDASELPEFLGGNCTCPEYGGCLKAEKGPWKDANILKKVLNGEAQCARQIVTVSNGMETIISYAKSKHQTVRGSDTSTAESGSEAEDVTSPKALRSYISHPKLTPVREEVKMIRATSFSTRMPEYDIPVVDKAVDATWKRELPRKAPFPSKDSSSLAATTKAPNRSLDQVIPALMAFVIAIVTLLRSVKDVATKRLPHKNESDEQSSSIYQDCIPKEEFRPPSPGPGFAESDLFSLVLQRLAELEAKVHALEEKPSEMPCEKEELLNAAVRRVDALEAELIVTKKALHEALIRQEELLAYVDRKTIAKAQKKKKKPMSCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BHS6</accession>
    <name>A0A8I7BHS6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123406369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044955815.1">
        <property type="nucleotide sequence ID" value="XM_045099880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BHS6"/>
    <dbReference type="SMR" id="A0A8I7BHS6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628630"/>
    </dbReference>
    <dbReference type="GeneID" id="123406369"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0628630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406369"/>
    <dbReference type="OrthoDB" id="1927826at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF111">
        <property type="entry name" value="F-BOX PROTEIN PP2-B12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="299" mass="32954" checksum="03484FB63B5D9DC6" modified="2022-05-25" version="1">MEEACEIARLPEELVSAALAHTSPRDACRAAAVSPAFRAAADSDDVWARFLPPGGLPPLADGEVAVPAPPSSKKELFLRLSAGPALLQDRLVSVWLDRETGAKCYMLSARNLFIVWGDTPQYWTWIPLEDSRFSEGAQLMSVCWFEIRGKIHSKMLSQNTTYAAYMVFKTTDNFYGLDFPVQEASISAGATNSTRKVCLQGNDDDGGGEGGVPENYLPMIPFPVRRLRRRNRRVVSHEENATFPQQRTDGWMELELGEFLNEGGGDDGEVSISLVETKGGNWKSGLIVQGIEIRRKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTH3</accession>
    <name>F2CTH3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444797</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK354925">
        <property type="protein sequence ID" value="BAJ86144.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977580.1">
        <property type="nucleotide sequence ID" value="XM_045121645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CTH3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0299540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0299540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0299540"/>
    </dbReference>
    <dbReference type="GeneID" id="123444797"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0249800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0249800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0249800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0299540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0299540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0299540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444797"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="126"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ86144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0299540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="275" mass="29456" checksum="F5453CB0B424BEF8" modified="2011-05-31" version="1">MSHNYGDTIPLHPSSAQSDMDEIESLMYDAPSATVLPARPPSPPRASIPISTSPPPPSSKPPLPASSVPIFVPQGPTPAGPPASVSVAIASDGFGPPPNTLTEPVWDTVKRDLARIVSNLKLVVFPNPNREDPGKALRDWDLWGPFFFIVFLGLTLSWSASVKKSEVFAVAFAVLAAGAIILTLNVLLLGGHIIFFQSLSLLGYCLFPLDVGALICLLKDNVILKIIVVTVTLAWSSWAAYPFMSAAVNPRRKALAIYPVFLMYISVGFLIIAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXK4</accession>
    <name>F2CXK4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyadenylate-binding protein</fullName>
            <shortName evidence="11">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123411286</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
            <tissue evidence="16">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK356357">
        <property type="protein sequence ID" value="BAJ87575.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK358958">
        <property type="protein sequence ID" value="BAJ90169.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960153.1">
        <property type="nucleotide sequence ID" value="XM_045104218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXK4"/>
    <dbReference type="SMR" id="F2CXK4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693350.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693350.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693350"/>
    </dbReference>
    <dbReference type="GeneID" id="123411286"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0575590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0575590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0575590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0693350.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693350.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411286"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000217">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000239">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000411">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="42"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="130"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="221"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="324"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="14">
        <location>
            <begin position="564"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="487"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAJ87575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ90169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693350.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="663" mass="71730" checksum="6DA758162446E820" modified="2011-05-31" version="1">MMAAQVQALAAPAAGAMEGVASPAAVAAAAAATATTTAFPATSLYVGDLDVSVQDAQLFDVFSQVGSVVSVRVCRDVNTRMSLGYAYVNFSSPADAARALEMLNFTPVNGKPIRIMYSNRDPSSRKSGAANIFIKNLDKSIDNKALYDTFSAFGNILSCKVATEMSGESKGYGFVQYEQDESAQNAINELNGMLLNDKKVYVGPFVRKQERENVFGSPKFNNVYVKNLSESTTEDNLKELFGNFGPITSVIVVRADDGKSRCFGFVNFENPDDAVHAVEDLNGKKFDDKELYVGRAQKKSEREMQLKESFEKSNKETADRNQGTNLYLKNLDGSVDDDEKLKELFAEFGTITSCKVMRDSNGVNKGSGFVAFKSSEDATRALVAMNGKMVGSKPLYVALAQRKEERRARLQAQFSQMRPVMPPPVAPRMPMYPPGVPGMGQQLFYGQPPPAFVNPQPGYGFQQHMIPGMRPGVAPMPNFVMPMVQQGQQPQRPSGRRAGAGGMQQPMPMGHQQMVPRGGRGGYRYASGRGMPEAAFRGVGGMAPSLYEMGRMTPGDTGAPQQVSSGALASALANSPPEQQRLMLGESLYPLVDQLEHDQAAKVTGMLLEMDQTEVLHLIESPDALKSKVAEAMEVLRSAQQQQTNVPADQLAALSLSDGFVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F2DC91</accession>
    <name>F2DC91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">heme oxygenase (biliverdin-producing)</fullName>
            <ecNumber evidence="3">1.14.14.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123410937</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heme oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.18" evidence="3"/>
    <dbReference type="EMBL" id="AK361508">
        <property type="protein sequence ID" value="BAJ92712.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959795.1">
        <property type="nucleotide sequence ID" value="XM_045103860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DC91"/>
    <dbReference type="SMR" id="F2DC91"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0719440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0719440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0719440"/>
    </dbReference>
    <dbReference type="GeneID" id="123410937"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0596670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0596670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0596670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0719440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0719440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0719440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410937"/>
    <dbReference type="OrthoDB" id="652091at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006788">
        <property type="term" value="P:heme oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19165">
        <property type="entry name" value="HemeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.910.10:FF:000005">
        <property type="entry name" value="Heme oxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016053">
        <property type="entry name" value="Haem_Oase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016951">
        <property type="entry name" value="Haem_Oase_decyc_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35703">
        <property type="entry name" value="HEME OXYGENASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35703:SF2">
        <property type="entry name" value="HEME OXYGENASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01126">
        <property type="entry name" value="Heme_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ92712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0719440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="288" mass="31591" checksum="5F60754A8F228596" modified="2011-05-31" version="1">MAPAAASASASIAAIAQHHAPAAPRASVSVAAAGRNGGLSVSVGVRPVPGISVVNSQRRRMVAAAAATEMAPAARGEGGGKPFVDEMRAVAMRLHTKDQAREGEKEPQAPPVARWEPTVEGYLRFLVDSKLVFQTLEDIVDRAAVPWYADFRNTGLERSEPLKKDLEWFTEQGHTIPEPTAAGTAYASYLEELSEKDPQAFICHFYNVYFAHTAGGRMIGKKVAEKILNKKELEFYKWEGTLSQLLQNVRTTLNQVASSWSREEKDHCLEETEKSFAYSGDLLRQIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F2DD77</accession>
    <name>F2DD77_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123425930</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ98292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="146" mass="16287" checksum="5DE6813D6EBAFB04" modified="2011-05-31" version="1">MRGEANGGDRRLRDEDGEEEEPPQRQEEEERAAKPSSGQQQQPAAAGAATTTTTRSVGYVGKHRLSAAIQRLDQELQSLQDELNELETMEPASAACREVITSTEGKPDPLLPITSSPENSSWDRWFQRVRSSRSNKWWQSKGSDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F2DVB6</accession>
    <name>F2DVB6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123452696</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK367834">
        <property type="protein sequence ID" value="BAJ99037.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985338.1">
        <property type="nucleotide sequence ID" value="XM_045129403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DVB6"/>
    <dbReference type="SMR" id="F2DVB6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518960"/>
    </dbReference>
    <dbReference type="GeneID" id="123452696"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0518960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452696"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF5">
        <property type="entry name" value="OS03G0795500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="2">
        <location>
            <begin position="22"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ99037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="21090" checksum="78C5C39F88D8ED5F" modified="2011-05-31" version="1">MAAAPAPTAAAPAPAPAAAAVPSASVPRGQVDLVDFIDWTGVECLNQDPAHGIANALKQGYREDEGLHLASDSDEQLLIYIPFMQVIKLHSALFKGPEEEGPKTVKLFSNREHMGFSNVNDFPPSDSVDLSSSHLLESKPVTLKYVKFQNVRSLTMFIEDNQSGADITKIQKIALYGTTVDTTNMKDLKKIEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2E206</accession>
    <name>F2E206_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK370177">
        <property type="protein sequence ID" value="BAK01378.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372316">
        <property type="protein sequence ID" value="BAK03514.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E206"/>
    <dbReference type="SMR" id="F2E206"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279370"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279370.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279370.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279370.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279370.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279370"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015995_0_0_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000594">
        <property type="entry name" value="RING/U-box superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF25">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RLIM-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="378"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK01378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="423" mass="46379" checksum="14826844CAAABD81" modified="2011-05-31" version="1">MEVVDLVSSDSEGEACAHSPDRKRPAREPADPSRGPGSLPVQTSRMAWPPQHPCQAVRKDKEKAGEGESAWAAGPPSLRGGSHGSGAGILGARPVGWDSWSAAAHKSRDERDETAGRCWGPWGDEICRSVSMPKEEPDNRDGAAMPGQSSMAIPELLMEDSSAWLSRIKGLHFPLPDASQLKARQIESDEMLALKLQEQLNQEHPGSQSSQQVDSTLAWTLHEEDAARARIAAREGQSSSSQRDRSMAHLYSYGWRSQVQGSTSWTASHTSAPTTSRRWLPRNSGPGSEQQNMIISQLTKGLFGEENMDLEMRMAVLDSLQDALESCADTYPTDSDDDDYENLIALDDNNHHRGASDSEIDSLPVSVAEGESRRDEPCPICLDCPADGVSLRHLPCAHKFHKECIDRWLRMRTSCPVCKSSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F2E376</accession>
    <name>F2E376_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442882</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Membrane</location>
            <topology evidence="2 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370599">
        <property type="protein sequence ID" value="BAK01798.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974988.1">
        <property type="nucleotide sequence ID" value="XM_045119053.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974990.1">
        <property type="nucleotide sequence ID" value="XM_045119055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E376"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236030"/>
    </dbReference>
    <dbReference type="GeneID" id="123442882"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442882"/>
    <dbReference type="HOGENOM" id="CLU_060198_2_1_1"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF2">
        <property type="entry name" value="PRA1 FAMILY PROTEIN F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK01798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="193" mass="20616" checksum="35E2801132D36AAB" modified="2011-05-31" version="1">MSKYGTIPTSSSSAAGAAHLGGASPLDFISRAKARGATALATRRPWRELADVHAIGLPPSLGDAYLRVRANLAHFAMNYAIVVLVVVFLSLLWQPISLIVFLVCMVGWLVLYFLRDEPVVLFGRVVGDGVVLAVLAVVTLILLLLTGATTNILTSLLIGFVLVVVHAALHKAEDNVDDEVGRWYAPVPAQPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F2E8E8</accession>
    <name>F2E8E8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123443745</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK372422">
        <property type="protein sequence ID" value="BAK03620.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976200.1">
        <property type="nucleotide sequence ID" value="XM_045120265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E8E8"/>
    <dbReference type="PaxDb" id="4513-MLOC_56183.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0267150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0267150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0267150"/>
    </dbReference>
    <dbReference type="GeneID" id="123443745"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0221410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0221410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0221410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0267150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0267150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0267150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443745"/>
    <dbReference type="eggNOG" id="KOG4223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038312_1_1_1"/>
    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E8E8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000297">
        <property type="entry name" value="Calcium-binding EF hand family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000328">
        <property type="entry name" value="Calcium-binding EF hand family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF53">
        <property type="entry name" value="CALCIUM-BINDING EF HAND FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="339"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK03620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0267150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="44820" checksum="E6FAD34C18BB56E1" modified="2011-05-31" version="1">MTPPPAVPPGRKSPAAVLFLCLVTTSLLMFILLASYTPRLEPHGRSPHRRLKLHPKNSAAVASSYGAGAVHESGGNRHAAPFDPAIAELERRLEDKEWEREHYRILHGDAEKGDHMKEWEDFLKEEEDFINDDDRFNISDRIRALFPKIDLSPEDGFVSLDELIRWNLDQARADQLHRSAREMELYDKNGNGIVSFTAFQTLRQQSHGDGNSLGFPWWKEEHFNASDVNGDGFLNKTEFHDFLNPSDSENPKIINLLCRQELRQRDKDGDGKLNFEEYFHGLHDHIHGYDDENAAISHIGNMTIAKERFSKLDKDNDGFISGHELEPVLDKLHLSERYYARQQATHAISEADKDHDGRLTLEEMIENPYAFYGSVYFSDDEDYFHEEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2EJ21</accession>
    <name>F2EJ21_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123411277</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="AK376148">
        <property type="protein sequence ID" value="BAK07343.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960144.1">
        <property type="nucleotide sequence ID" value="XM_045104209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EJ21"/>
    <dbReference type="SMR" id="F2EJ21"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0691600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0691600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0691600"/>
    </dbReference>
    <dbReference type="GeneID" id="123411277"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0574190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0574190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0574190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0691600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0691600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0691600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411277"/>
    <dbReference type="HOGENOM" id="CLU_006348_5_1_1"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.20:FF:000002">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000082">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000335">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF359">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="273"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="407"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK07343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0691600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="644" mass="71362" checksum="8BD8A9F19B9EA6AF" modified="2011-05-31" version="1">MPQPVLPPSSAATADPEPSRAGAREMDDEDLVEELLATVNSARAYSEFRRTQRKECHNLLRWLQLILPLLEELRDSAPHLTDDAYRRLTLLGRALAAARRLLRSCNDGSKIFLALESEAVLGRFRTVYEKMNSALDGMPYAELGISDEVTEQVELMNAQLMRCKKRTDTQDIELSMDLMVILQNNKDEERNADRAILDRLASKLELQMLADLRAETVAIKKLINERNGQHADSTKQIVELLHKFKAIAGIEEKNVLGSEVFVTKSLDKCPSLMIPDDFLCPITLEIMTDPVIVASGQTYERRSIQKWLDSGERTCPKTRQPLVHLSLAPNYALKNLILQWCDKHKVELQRREPEPVAEQDGHPREDIPSLVEALSSIHPDVQRKAAKKIRMLSKESPENRALIVGNGGIPALIGLLAYPDKKVQENTVTSLLNLSIDHSNKLLITKGGAIPLIIEILRNGSAEGQENSAATLFSLSMLDENKATIGTLGGITPLVELLTNGTVRGKKDAATAIFNLILNQQNKVRATQAGIVPSLMKVMDDRSLGMVDEALSIFLLLSSHPTSVGEIGTTPFVEKLVQLIKEGTPKNKECALSVLLELGSKKQTLLVHALRFGLHEHLSQIAKTGTSRAQRKANSLIQIAKKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2EK12</accession>
    <name>F2EK12_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123446941</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Seed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK376489">
        <property type="protein sequence ID" value="BAK07684.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979422.1">
        <property type="nucleotide sequence ID" value="XM_045123487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EK12"/>
    <dbReference type="SMR" id="F2EK12"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333260"/>
    </dbReference>
    <dbReference type="GeneID" id="123446941"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446941"/>
    <dbReference type="HOGENOM" id="CLU_029813_0_0_1"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16620">
        <property type="entry name" value="vRING-HC-C4C4_RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF6">
        <property type="entry name" value="OS03G0659400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="9">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="550"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="604"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK07684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="731" mass="80148" checksum="AB9B656C3E57E763" modified="2011-05-31" version="1">MGVLYYKYKSAKETYPVQLPYSFISVSELKQLILTSNRHGIGRSRGRGPREDIALSNAQTGEEYADENAMIPQNVTVLVRRTAGQESENIVVISSRKVIEDGSIASNRSVVTESVSKSCSSAEMKDEDAAIAAVIDAAELKWEEQSFKRGQAPGRFISGRQNGHGSSEREAPPPGYVCRSCGVAGHFIQHCPQENQTPPPGYTCYRCRIPGHFIQHCPTIGDSKFDNYKMSRPVAPVVPLNPVDGILSALAPAASVSEVDDLPAELHCQLCNKVMADAVLTSKCCFVSFCDKCIRDYIITQSKCICGVKVLADSLIPNPTVRSTISNLLGTRTCSTASGTGKHRSSSGSNADPKSQSHTTSAALERDTKQCLDSQLSPAASDARLQVATEHDQVDRPQKNSDLKSNTEGSAGRSAEKAVPSADLPKPKDVSESTLKGTAISSGTLEPKVARSDQLKKKRKKADSSKAVHPNNADYGYNVPFDPSYCNPFNGGYVMGPFGVPEPYMYGSMGMPYGGFPMGPFGMNPFGNIPPQALAMQGYPPNYQSWETQAAVHRDAEAAARARQVSHGNADAAVRSRQTERPKDSGAWPRPSERNQRMGSPQGRELRDRPQSRPEPSERNQRLGSPRGRESRERSRSRSKPSERSQRSGSPRGRESRDRSQSRPARSRDHGRNDRGSSDYHEDHHDRKRMRASSTVDGDTDSSQRSKHSSRSLRRDDGSDDEQNFKRKWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0W5Z8</accession>
    <name>M0W5Z8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123439405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972061.1">
        <property type="nucleotide sequence ID" value="XM_045116126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W5Z8"/>
    <dbReference type="FunCoup" id="M0W5Z8">
        <property type="interactions" value="964"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W5Z8"/>
    <dbReference type="PaxDb" id="4513-MLOC_44131.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232780"/>
    </dbReference>
    <dbReference type="GeneID" id="123439405"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439405"/>
    <dbReference type="eggNOG" id="ENOG502QUC8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W5Z8"/>
    <dbReference type="OMA" id="WEYDNKD"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W5Z8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF25">
        <property type="entry name" value="OS01G0100600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="36138" checksum="0067FFE9E417C18C" modified="2013-04-03" version="1">MDTTQFAMIEELASLVKDNLYSKHLVLSTEEALVTLLQHNYLDDDDEHDRTTTGASHGAAGNTMELQPTSSYNRLLLHRLADIYGFAHESVGEGEDRHLVLQRCPETAIPPVLVSDVLWNYDDSDGPSASLMLARNETDLQKTYEPEDVQDAISLESLHLKTDTPVSKPLQQSVPLSAASLKEREAAYRAARERIFSTHEAKGKDTSAAKSRHVPAVAQRMIAHALGKRVEDPTERAALVNVKGKELVNGRTSMDNQHAVKPDNKEAAKHNLSSPGGGPYWNLSSQGRPTPTVSAETLKKEQTGAAKRMFAHALRLPGVEISNGAARKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0W6X4</accession>
    <name>M0W6X4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123425430</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965067.1">
        <property type="nucleotide sequence ID" value="XM_045109132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W6X4"/>
    <dbReference type="SMR" id="M0W6X4"/>
    <dbReference type="FunCoup" id="M0W6X4">
        <property type="interactions" value="962"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W6X4"/>
    <dbReference type="PaxDb" id="4513-MLOC_44338.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128200"/>
    </dbReference>
    <dbReference type="GeneID" id="123425430"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0105750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0105750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0105750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425430"/>
    <dbReference type="eggNOG" id="KOG2743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W6X4"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W6X4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="364" mass="40773" checksum="8DC7660CDA8A6ABE" modified="2013-04-03" version="1">MEDDDDCPPLAVELPPQRTSPLVPASSSASAPVGVTVITGYLGAGKSTLVNYILSGQHGKRIAVILNEFGEEIGVERAMINEGQGGALVEEWVELANGCVCCTVKHSLVQALEQLVQTKERMDHILLETTGLADPAPLVSILWLDDQLESSIRLDSIITVIDAKNFRRQIDEHTNSSSFPEAFHQIAFADVVILNKIDLVKDDLEDLERQIHDVNELVTVVRSVRCQVDLNTIFDRQAYGVKNSSQLQELLEYSKSAPPNSRHDNSISTLCIHEQDPVNLAKVESWLEDLLWEKKSNMDIYRCKGILNIHNSNQLHTLQAVREVYEVVPAREWSETQPRMNKIVFIGRDLDISTLQDSFSRCKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y8I4</accession>
    <name>M0Y8I4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123442853</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974955.1">
        <property type="nucleotide sequence ID" value="XM_045119020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y8I4"/>
    <dbReference type="SMR" id="M0Y8I4"/>
    <dbReference type="PaxDb" id="4513-MLOC_6671.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235170.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235170.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235170"/>
    </dbReference>
    <dbReference type="GeneID" id="123442853"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0235170.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235170.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442853"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="GIQPWIN"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y8I4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235170.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="469" mass="52091" checksum="C3BB83C4F671F560" modified="2013-04-03" version="1">MKDRSHGLALRVSLLLLVLIIIVIAQAQSEKNQTTVQVSHLPGFHGPLPFSLETGYVQVDNRNGVHLFYYFVQSETSPTEDPVLLWLTGGPGCSAFSGLVYEIGPLSFQPDSYTGGLPELVYRPDSWTKVANVIFLDSPVGAGFSYSATDQGYKSSDTKAVDQIAIFLTKWYKEHPQFLLNPLFIAGDSYSGLIGPPLTVQIAKGIEMGDKPLLNLKGYIIGNPLTDRKFDLPARVPYAHRMGLISDEQYEKYRESCGADTGMNRNIQCKNCHDAIDKCLKGINIHHILEPECSSEYNGNSDSGRTLLDYRGAELGLSDISSECREKGYSMSSIWANNRAVREALGVHNGTVPVWLRCNHGTPYTTDIRSSVEYHRSLASRGYRSLIYSGDHDMTVPFIGTQAWIRSLRFAIVDQWRPWYATGQVAGFTTLYANNLTFATVKGGGHTAPEYRPKECLAMVDRWLSGRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>M0YC53</accession>
    <name>M0YC53_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123425358</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964980.1">
        <property type="nucleotide sequence ID" value="XM_045109045.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964981.1">
        <property type="nucleotide sequence ID" value="XM_045109046.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964982.1">
        <property type="nucleotide sequence ID" value="XM_045109047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YC53"/>
    <dbReference type="SMR" id="M0YC53"/>
    <dbReference type="PaxDb" id="4513-MLOC_67781.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0126220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0126220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0126220"/>
    </dbReference>
    <dbReference type="GeneID" id="123425358"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0104130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0104130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0104130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0126220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0126220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0126220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425358"/>
    <dbReference type="eggNOG" id="KOG1561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YC53">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018362">
        <property type="entry name" value="CCAAT-binding_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00686">
        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0126220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="300" mass="32750" checksum="A3530A081AD164DD" modified="2013-04-03" version="1">MEDHPGHPISNYDFLSGNGCHSKKLVDKNYDQDSSSTKSDRSQQEAPAMTDSSLNEQHVSRPSSQSDNDNDHGKPDQHMIKSLLSLGNPDTVAPPPMLACSQSFAYIPYPADAYAGVFPGYASHAIVHPQLNAATNSRVPLPVEPAAEEPMFVNAKQYHAILRRRQIRAKLEAQNKLVKARKPYLHESRHRHAMKRARGTGGRFLNTKQLEEQKQKQASGGASCTKVLGKNTVLQSSPTFAPLASAPSNMSSFSTTGMLANQERACFPSLGFRPTVSFAALNGNGKLAPNGMHQRASMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0YHL4</accession>
    <name>M0YHL4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123399991</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950315.1">
        <property type="nucleotide sequence ID" value="XM_045094380.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950316.1">
        <property type="nucleotide sequence ID" value="XM_045094381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YHL4"/>
    <dbReference type="STRING" id="112509.M0YHL4"/>
    <dbReference type="PaxDb" id="4513-MLOC_69412.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497490"/>
    </dbReference>
    <dbReference type="GeneID" id="123399991"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0412660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0412660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0412660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399991"/>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YHL4"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YHL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14479">
        <property type="entry name" value="SPX-MFS_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051068">
        <property type="entry name" value="MFS_Domain-Containing_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045264">
        <property type="entry name" value="SPXM_SPX_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YAJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510:SF65">
        <property type="entry name" value="SPX DOMAIN-CONTAINING MEMBRANE PROTEIN OS09G0521800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="694" mass="76193" checksum="396D5F3D5CAC8ADA" modified="2013-04-03" version="1">MVKFSKKLTTDQVPGWEEYYFNYKLLKARVKVYTEQTKEGNHDRRRVLKDFSKLLDDEIEKIVLFMIEQQGLIAARLEELGKRRAVLEDIPLLQEITELREDYRAVGHDLVRLLKFVDLNANAVRKILKKFDERLGYKFTDYYVRSRSNHPYSQLQQVFKHVGIGAVVGALSRNLGDLEEREGSYLNIYDQHPLAIPKDPIIDMIKATADKLTNSTNFLRFLGQHALIAQGSIADDSPEEQQVSEDKYHFISLVLNLANTFLYMVNTYIVVPTADDYATSLGAAATVCGVIIGSMAVAQLFSSVYFSAWSNRSYFRPLLFSSVVLLLGNVMYALAYDLDSLAILLAGRVLCGMGSARAVNRRYISDCVPQRIRMQASAAFVSASALGMACGPAIAGLLQVNFRLYAVTINQDTLPGWVMAFGWLAYLLWLWISFKEPVLGDAGDEGHRQGSSRGSSSLGYRKQGLAGEYLLKQDAEGESEEEETPAPAAAPSIAEAYGLLTPSVKVQLLIYFMLKFSMEILLSESSVVTSYYFGWNTSTVAVFLAALGLTVLPINAVVGTYISNMFEDRQILVASEAVLLAGVALSFHVPGTAYTAAQYVCSALLTFVAAEVLEGVNLSLLSQVMPARLSRGTWNGGLLSTEAGTLARVAADGTITLAGYLGQGALLNATLLPSLLICAASIAATLSTYNSLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287TFN9</accession>
    <name>A0A287TFN9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123401925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951726.1">
        <property type="nucleotide sequence ID" value="XM_045095791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287TFN9"/>
    <dbReference type="SMR" id="A0A287TFN9"/>
    <dbReference type="FunCoup" id="A0A287TFN9">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287TFN9"/>
    <dbReference type="PaxDb" id="4513-MLOC_12352.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553470"/>
    </dbReference>
    <dbReference type="GeneID" id="123401925"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0459760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0459760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0459760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0553470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401925"/>
    <dbReference type="eggNOG" id="KOG2699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287TFN9"/>
    <dbReference type="OMA" id="CANAKDH"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287TFN9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd10461">
        <property type="entry name" value="PUB_UBA_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14290">
        <property type="entry name" value="UBA_PUB_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46713">
        <property type="entry name" value="F13M7.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46713:SF1">
        <property type="entry name" value="F13M7.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="411" mass="46205" checksum="E7A40485454C8BAB" modified="2017-11-22" version="1">MAGLSLRCGDCGVQLRSVEEAQAHAEATSHTNFAESTEAVLNLVCADCGKPCRSQTEVDLHKKRTGHAEFTDKTMEAAKPIDLEAPPKPAAEAMDVDASGSAEPQELVAPEVNKEMLGELESMGFSTARATRALHFSGNSTIEGAINWLSEHQEDPDIDEMPMVPANSEANKPSLTAEEKKIKAQELRERARKKKEEEERRMEREKEKERIRIGKELLEAKKMEELNERKRIIELRRLEKEEEKRAREKIRQKLEEDKAERRRKLGLPAEAPAASKPSAPPPVEEKKSAFLPVRPATKAERMRDCLRNIKQQNKEEDAKVKRAFQTLLTYIGNVVKNPDEEKFRKIRLTNATFQERVGSLGGIEFLELCGFEKPEGEEILFLARDKVDKAVLNVAGAELNSAITNPFFGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287U1Y5</accession>
    <name>A0A287U1Y5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123402722</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952609.1">
        <property type="nucleotide sequence ID" value="XM_045096674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287U1Y5"/>
    <dbReference type="SMR" id="A0A287U1Y5"/>
    <dbReference type="PaxDb" id="4513-MLOC_67722.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582230"/>
    </dbReference>
    <dbReference type="GeneID" id="123402722"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0482710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0482710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0482710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0582230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402722"/>
    <dbReference type="eggNOG" id="ENOG502RKDV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FMLRPEK"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287U1Y5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF671">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41384" checksum="70E4F6F50CE294CC" modified="2017-11-22" version="1">MGRWEALVAIAVVAVFLAAAAEKVDAAKAAAKGKYQALFNFGDSLADAGNLIANGVPDNLTTARLPYGQTYFGKPTGRCSDGRLVIDHLAQEFGLSLPPPSKANHSDFKHGANFAITGATALDTPYFEVRGLGAVVWNSGALMTQIQWFRDLKPFLCNSTKEECKEFYANSLFVIGEFGGNDYNAPLFAGKGLTEAYKFMPDVIQGISDGVEELIAEGAADLIVPGVMPTGCFPVYLNMLDMPAHEYGSQSGCIRQYNTFSWVHNEHLKRALEKLRPKYPNVRIIYGDYYTPVVQFILQPEKFGFYKQLPRACCGSPGSVAKAVHNFNVTAKCGEPGATACADPSTHWSWDGIHLTDAAYGHIAKGWLYGPFADQPIVQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287XAS7</accession>
    <name>A0A287XAS7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123410459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959341.1">
        <property type="nucleotide sequence ID" value="XM_045103406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287XAS7"/>
    <dbReference type="FunCoup" id="A0A287XAS7">
        <property type="interactions" value="1857"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721230"/>
    </dbReference>
    <dbReference type="GeneID" id="123410459"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0598150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0598150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0598150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0721230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410459"/>
    <dbReference type="eggNOG" id="KOG2819">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287XAS7"/>
    <dbReference type="OMA" id="YRKNDQK"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287XAS7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="425" mass="47065" checksum="D46A700A50D5D8A4" modified="2017-11-22" version="1">MQTQRSPAMVGGGPGSAVGSPSPATVPVRRRCEGTAMGAVTLDLRPGLGVGPFTLGMPISDAFAQIEHQPNIYDVVHVKYFDEEPLKLDFVISFPDHGFHLRFDPWSQRLRLIEIFDVKRLQLRYATSLIGGPSTLATFVSVYALFGPTFPGIYDKERGMYTLFYPGLSFAFPIPSQYTNFFNNGEVADLPLEFPDGTTPVTCRVSIYDSSTDSKVGVGSLMDKAVIPALPAGSLYMEEVHAKLGEELWFTIGGQHIPFGASPQDIWTELGRPCGIHQKQVDQMVIHSASDLRPRTTLCGDYFYNYFSRGIDILFDGQTHKIKKFVLHTNFPGHSDFNSYMKCNFVIYDAEGGQQPTGNTSKNCITPRTKWEQVKEILGDCGRAAIQTQGSMNNPFGSTFVYGYQNIAFEVMKNGYIATVTLFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WBV7</accession>
    <name>A0A8I6WBV7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WBV7"/>
    <dbReference type="SMR" id="A0A8I6WBV7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0029680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="27012" checksum="1A127C0E9C9D0C8B" modified="2022-05-25" version="1">MATFIPVKFTEFSQPDATMPFGKELVQITKDLRIALPTITGKPTSSYPEDSRYRIEVHVPGRTFQPRTEPKDFKFIAPNWILRRDMAIHCALGRIKKEYKGCPISPDLFTVSRRNEDGEIISSKTKDALLSYAQTLEKHSGTLEHHVIKDARKIKQLSLHELELEDAAREAHYEHELEVKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPITMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCA0</accession>
    <name>A0A8I6WCA0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123425844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965525.1">
        <property type="nucleotide sequence ID" value="XM_045109590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCA0"/>
    <dbReference type="SMR" id="A0A8I6WCA0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139470"/>
    </dbReference>
    <dbReference type="GeneID" id="123425844"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0114920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0114920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0114920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0139470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425844"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000025">
        <property type="entry name" value="TBC1 domain family member 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF659">
        <property type="entry name" value="OS07G0525400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="67"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="446" mass="50771" checksum="7DC8B8F1C200A349" modified="2022-05-25" version="1">MIKWGISSGTPADSYYEIRSDCTDDVPKSKFKIKAGKTLSVRKWQAAFNPDGCLDIASVLSRIQKGGVHPTVRGEVWEFLLGCFDPRSTFDEREEIRQIRRLQYARWKEECREMDSHVGSGKVITAPLITEDGRPIKDPLVLLEATSNQNTSDGASTSSNNGIEVDDSAERITDKQIIEWKLTLHQIGLDVLRTDRTMVFYENKENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMIVLLNDEADAFWCFEKLMRRLRGNFRCTDQSVGVANQLQHLASIIQVLDPKLHDHLETLGGGDYLFAFRMFMVLFRREVSFGDSLYLWEMMWALEYDPDIFFAACEEASGAHKKVSKSKLRGVRHFAKWDKDKDKDVPEETDGPVPISVFMVASVLKEKREKLLQEARGLDDLIRILNDVNGNLDAKKACAGALKLHKKYLKKVQAKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCB5</accession>
    <name>A0A8I6WCB5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123431407</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
            <location evidence="13">COPI-coated vesicle membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971132.1">
        <property type="nucleotide sequence ID" value="XM_045115197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCB5"/>
    <dbReference type="SMR" id="A0A8I6WCB5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0044090.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0044090.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0044090"/>
    </dbReference>
    <dbReference type="GeneID" id="123431407"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0034210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0034210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0034210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0044090.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0044090.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0044090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431407"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000071">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000078">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000002">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000011">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="15">
        <location>
            <begin position="28"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="16">
        <location>
            <begin position="624"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="17">
        <location>
            <begin position="772"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="584"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="605"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0044090.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="889" mass="98751" checksum="0ACF06FB0E23D1C0" modified="2022-05-25" version="1">MSQPLVVKKDDDLDEEEYYSPFLGIEKGAVLQEARVFHDPQLDARRCCQVITKLLYLLNQGDTFTKVEATEVFFATTKLFQSKDAGLRRMVYLMIKELSPSADEVIIVTSSLMKDMNSKTDMYRANAIRVLCRIIDSTLLTQIERYLKQAIVDKNPVVASAALVSGIYLLQTSPEVVKRWSNEVQEAVQSRAALVQFHALALLHQIRQNDRLAVSKLVTSLTRGSVRSPLAQCLLIRYTSQVIRESGMNTQGGDRPFFDFLESCLRNKAEMVILEAARAITDLNGVTSRELTPAITVLQLFLSSSKPVLRFAAVRTLNKVASTHPLAVTNCNIDMESLISDQNRSIATLAITTLLKTGNESSVDRLMKQMTNFMSDIADEFKIVVVEAIRSLCLKFPLKYRSLMNFLSNILREEGGFDYKKAIVDSIIILIRDIPDAKESGLFHLCEFIEDCEFTYLSTQILHFLGNEGPKTSDPSKYIRYIYNRVILENATVRASAVSTLAKFGALVDALKPRIFVLLRRCLFDGDDEVRDRATLYIKLLDGEATVGETEKDVTDFLFGSLDVPLANLEASLRNYEPSEVPFDISSVPKETKSQPLAEKKATGKKSTGPSSTVNGPVPTIDASYEKLLSAIPEFAGFGKLFKSSTPVELTEAETEYSVNVVKHIYDGHVVLQYNCTNTIPEQLLEEVMVFVDASEAEEFSEVASKPLRSLPYDSPGQTFVAFEKPEGVLATGKFSNILKFIVKEVDSSTGEAEDDGVEDEYQLEDFEIISADYMLKVGVSNFRNAWESMDPESERVDEYGLGVRESLAEAVSAVISILGMQPCEGTDVVQNNSRSHTCLLSGVFIGNVKVLVRLSFGISGPKEVAMKLAVRSDDPEVSDRIHEIVANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCG5</accession>
    <name>A0A8I6WCG5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCG5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0000520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1135">
        <property type="entry name" value="OS08G0246300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="51"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="410"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="98057" checksum="0F9322ADBFB2D3C3" modified="2022-05-25" version="1">MLSVEDLMKDQSGLADGKGNIKEEGESEPYTISGDVDRDRVFYDLHAGKKLSVWGIAGLGKSSLVKHHYNKIKSMQLYAMYGWVEVPQPFNLMDLCQRLLLDLLSDDVHDKEAVAIGLFGGQDPIEVCIKILLEHKCIVVFDGLGSTDDWDVINKTLFSQPISGGTIVITRQEKVARHCSQDNVYNTYGLEPDVALDLFTTIILNGKRLFPDAEEASRLMLSKCGGLPEVIIAIGKQIHPKAQQKDSLLRILNEINVDFIGYLQLHLPSLRGLFCWMKSYFDTCSDDHKPCIFYMSIFPADERIRWRRLLRRWIAEGYSCNEETAGDLAMELNNSSIMYYGDGNEISIRVKFKLNGFFLEYVKSQPMEHNRVFALDGSCSPSSRLTGQHLTITSSWDRDKIVFQSLDMSKLRSFTVFGEWKSFLICKKMKLLRVLDLEGTTTSDSTSSVSDDDLEKIAELFPRLKVISIRGCKGITRLPDSMGDMKQLNTLDAKNTSIVELPPAIITKLHKLQYIRVGSGTSDSEESEPEVLAGTLFPHAPPQEDGPSGTSPFLRAKKALSGVVQGASTGARKMAHQSCSKLRIFKNDVIRQHVTENGGGVEVIPAAAERIGELTELRTLGVVNVAYVPGALRFFKELKKLTQLRKLALSGITRENWKMLCCAISGHRHLEILWLQLLLLKEDNASYDFARFDDISKPPMTIQRLKVLYTSKAVAGAGYAWITPTWIKQLGKIRFGHELTVSSQEDIDLLYESPGQLPGSYDRLHIKPTEDLIFHKGLSYNALSITCNGRMSTVAFRGTDTSETSENSDFKAKELKIHSCRSCGLRCLRISGLKHFKNLDNIRVTGQCSNEYEQDLRRQFEEHTENHKLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDK7</accession>
    <name>A0A8I6WDK7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123405678</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955234.1">
        <property type="nucleotide sequence ID" value="XM_045099299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDK7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013220"/>
    </dbReference>
    <dbReference type="GeneID" id="123405678"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0010090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0010090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0010090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0013220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405678"/>
    <dbReference type="OrthoDB" id="1881997at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32843" checksum="714B333A4961A799" modified="2022-05-25" version="1">MHGGGSKMLGVVNFITFLASIPVLGGGIWLASRANTTDCIRFLQWPIIIIGLAVMVVSLMGFAGACYRQTWLLRIYLFAMFFIVVALLFFIVFAFAVTDRGEGQVVMNRRFLEYQLSDYNGWLRGRVADPDYWATISACLRDGHACRSMRRPVRDPNTGMLVLEPAVVFYGRDLSPIQSGCCKPPTSCAFTYNNETYWSANPGVPTVVTDPDCLKWSNDQQTLCFQCDSCKAGVLAGIKKSWRKVAIINIVVLIILVIVYVAGCAAFRNAKRDDNDESYGMARMTKSRPSRFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WP25</accession>
    <name>A0A8I6WP25_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WP25"/>
    <dbReference type="SMR" id="A0A8I6WP25"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0115710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0115710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0095070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0095070.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0095070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0115710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0115710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0115710"/>
    </dbReference>
    <dbReference type="OMA" id="AHIDYAN"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035199661" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="30"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="72"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115710.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="576" mass="63161" checksum="46E8BEC5F633F839" modified="2022-05-25" version="1">MPRSSMAPSRIMALLAMLLVSRLFFCSQASSHGFLECVTTSVPGQLLFTRSSPSFASVLASSVRNRRFLTQSTVRPLCVVTATSASHVQAAVVCGRRHGVRLRVRSGGHDYEGLSFRSACPETFAVIDLAGLRAVRVSLGSPLEGAGAPATAWVDSGATLGELYYGIGKASDRLAFPAGLCPTVGVGGHLSGGGFGMLLRKHGLAADHVVDATMVDAEGRILDGDAMGRDVFWAIRGGGGGGSFGIVLSWRVMLVAVPPKVTAFTVHKSVEQGAVDMLTKWQEVAPALPDDLFVRVLVQRQVAKFQALYLGTCDALLPVMRRRFPEFGMNRTHCKEMTWLQSVPYIYLGIGATVEDILNRTDPVDATSSKATSDYVRHAIARDVWEEIFATWFARPDAGLMILDPYGGNMARVPEAATPFPHRAGVLYNIQYMNFWTAAGDGAAQTAWIRGVYAFMEPHVSKNPREAYVNYRDLDLGANVVVGNVTSYEAGKVWGEKYYKDNFRRLAMAKRQIDPDDYFRNEQSIPPLDAHEQRLIMRDVAPRGYANPVSMNHFYYILGCVCIFLMYIQRNYGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQ57</accession>
    <name>A0A8I6WQ57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426949</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966806.1">
        <property type="nucleotide sequence ID" value="XM_045110871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ57"/>
    <dbReference type="SMR" id="A0A8I6WQ57"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174010"/>
    </dbReference>
    <dbReference type="GeneID" id="123426949"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0143820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0143820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0143820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174010"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426949"/>
    <dbReference type="OMA" id="HDYKSID"/>
    <dbReference type="OrthoDB" id="1858121at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051761">
        <property type="entry name" value="MLP-like_ligand-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31907">
        <property type="entry name" value="MLP-LIKE PROTEIN 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="3">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="17134" checksum="4548AC66BF12FC54" modified="2022-05-25" version="1">MASKVELVVEVKSPADKLWTGMRESTELFPKIFPEQYKSIETVEGDGKSAGTVRLIKYTEGVPMVTFAKEKVEVADDEKKLVSYSVVDGELVSFYKNFRVTVQVTDKGSADGVGAVVNWTMDFDKASDEVPEPDVIKETAAKTFHDLDDYLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQI8</accession>
    <name>A0A8I6WQI8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123439560</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972195.1">
        <property type="nucleotide sequence ID" value="XM_045116260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQI8"/>
    <dbReference type="SMR" id="A0A8I6WQI8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0058170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0058170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0058170"/>
    </dbReference>
    <dbReference type="GeneID" id="123439560"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0046050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0046050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0046050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0058170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0058170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0058170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439560"/>
    <dbReference type="OMA" id="HCPTWSW"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141046">
        <property type="term" value="F:Atg8-family conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.50:FF:000007">
        <property type="entry name" value="Autophagy-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0058170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="316" mass="35635" checksum="A5592D43F78ABC8A" modified="2022-05-25" version="1">MQVKQKVYELYKGTVERVTGPRTVSAFLEKGVLSVPEFILAGDNLVSKCPTWSWEKGDPSKRKPYLPSDKQFLVTRNVPCLRRAMSVEEEYDAAAAEVVLDDDDDGEGWLATHGVQASESKEEEDIPSMDTLDIGKVEEIKSIPSYFGASEKPDEEEDIPDMDTYEDTGDHSTATPQPSYFVAEEPDDDNILLTRTYDVSITYDKYYQTPRVWLTGYDEARMPLKPDLVFQDISQDHAHKTVTIEDHPHLLAGQHASVHPCKHAAVMKKIIDVMMSQGGTPEVDKYLFIFLKFMASVIPTIEYDYTMDFDLGSPST</sequence>
</entry>
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    <accession>A0A8I6WST4</accession>
    <name>A0A8I6WST4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WST4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0130380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035266326" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="20308" checksum="32DFCE407B03CBBB" modified="2022-05-25" version="1">MAPTRSLVLLVAYTVLAAVMLQPSEERVQTAKAPSAPHLAGGKQAEAVVATSSPASPFTGVPQLPPLPGLPPLAGLPALPPSPGFPGMAGTQPSPSPSQQPAITSCLGPLILVAPCARYLTNSSVRAPPRTCCDGLRSLVGGGNRICLCHAIMGDLGTFAGGTIDQLRMLVLPLTCSTFVPPDLLLMCIVNPVPPILPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WV15</accession>
    <name>A0A8I6WV15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123440569</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044973069.1">
        <property type="nucleotide sequence ID" value="XM_045117134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WV15"/>
    <dbReference type="SMR" id="A0A8I6WV15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303990"/>
    </dbReference>
    <dbReference type="GeneID" id="123440569"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253710.1">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cts1-like"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
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        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035248676" description="chitinase" evidence="10">
        <location>
            <begin position="33"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="33"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303990.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="308" mass="32223" checksum="5F0CE6028AD7DF04" modified="2022-05-25" version="1">MAVPAKASSSFSTACQLLAAILLLSSAPRSHGGSIAIYWGQNGNEGTLAETCGTGNYAFVNIAFLCSFGSAQQAPQLNLAGHCDPYSNACTNLTADINLCQSKGVKVMLSIGGGAGGYTLNSEQDAADLARYIWDGFLGGSSPKRPLGAAVLDGVDFDIEGGNPDYYGALAAHLKSYGGKGGGKEVYLSAAPQCPFPDQWVGKALETGLFDYVWVQFYNNPPCQYVQGDTANLMDSWKQWTSGVHAKYIFLGLPAAPAAAGSGFIPAASLESQVLPALKGSSKYGGVMLWSKFYDDQDGYSSAIKKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZY7</accession>
    <name>A0A8I6WZY7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123424370</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044963909.1">
        <property type="nucleotide sequence ID" value="XM_045107974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZY7"/>
    <dbReference type="SMR" id="A0A8I6WZY7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099170"/>
    </dbReference>
    <dbReference type="GeneID" id="123424370"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0081690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0081690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0081690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0099170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424370"/>
    <dbReference type="OrthoDB" id="1663137at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000073">
        <property type="entry name" value="12-oxophytodienoate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF125">
        <property type="entry name" value="NADH:FLAVIN OXIDOREDUCTASE_NADH OXIDASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="12">
        <location>
            <begin position="12"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="369" mass="40722" checksum="2B276EFB9211C378" modified="2022-05-25" version="1">MEPEAESKPIPLMTPYKMGSTLDLAHRVVLAPLTRQRSYGNVPQPHAAVYYGQRATAGGMLITEATGVSDTAQGYTDTPGVWTAEQVEAWRPVVDAVHAKGAAFFCQLWHVGRVSTYGFQPGGAAPVSSTERMVGPQVRHDGSREEFSPPRRLAVEEIPLIVDDFRKAARNAIDAGFDGVEIHGGNGYLIEQFLKDSANDRDDGYGGSLENRCRFALEVVAAVVKEVGAHRVGIRLSPFMDYMDCHDSDPHALGLHMATKLNDHGILYLHMIEPRMALVDGRRVVPHRLLPYREAFKGTFIANGGYDREEGDKAVSSGYADLVSFGRLFLANPDLPKRLELNAPLNKYNRMTFYISDPVVGYTDYPFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0T7</accession>
    <name>A0A8I6X0T7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123448423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981219.1">
        <property type="nucleotide sequence ID" value="XM_045125284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0T7"/>
    <dbReference type="SMR" id="A0A8I6X0T7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0364120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0364120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0364120"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0364120.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123448423"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="34"/>
            <end position="361"/>
        </location>
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        <location>
            <begin position="116"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0364120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="361" mass="40147" checksum="B6712A1128214548" modified="2022-05-25" version="1">MAPLRRAATAAEVLLALTGLLLLVLSPAGAATAAVEGGELRMKQSDGGYSYNSTLAHVLVEYASAVYTSDLTSLLTWTCPRCEGHTKGFEMIEIIVDIEKCLQAFVGVAPDPQSIIIAFRGTQEHSASNWIEDLFWKQLDVTYPGMPNAMVHHGFYSAYYNTTLRHEILKSVQWAWKIYGRLPINVVGHSMGGALASFCALDLSVKWGSHKVQLITFGQPRVGNPAFAEYFNEQVPRTIRVTHENDIVPHLPPYFYYLGEWTYHHFAREVWLRETIVGNVVTRNATVCDCSGEDPICSRSVYGRSVADHLEYYGVNLHADSRGTCQFVIGASNSAYGDILQVDGTIILSRYLQERYPVESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6I5</accession>
    <name>A0A8I6X6I5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
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        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0252720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="67" mass="7217" checksum="E657EBF1A672BCD5" modified="2022-05-25" version="1">MTGPEAVVKIHAMGEGDVKIVGVSADDHAMEAFMNVGADAFVPKPMRREVLIPIIQDIINKNNNAMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
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    </dbReference>
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            <begin position="118"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0263750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="250" mass="28883" checksum="0F447A86F105A8BF" modified="2022-05-25" version="1">MAGLDPQFSPEKRNLFHASTFMIVGDGLTALFWEDRWLHGQSIRELAPLLYLCIPKNRRRERTVAEGIAGNAWARDIRGVVGLQEIGQYLRTWQLVARTNLSSEPDKLIWKWTANGIYSARSCYRATFHGSLTCHTWQLIWKGWAPPKVKFFHWLANLDRCWTAARLARRGLPHHTRCLLCDQEPETIQHLLISRSFARQSWHETLSWLHLPVPLRGMTLPSRIGGVGRETPPHHRSARHCDLSRCWSPG</sequence>
</entry>
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    <accession>A0A8I6X8Q4</accession>
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        <recommendedName>
            <fullName evidence="6">DUF547 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123430094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044969943.1">
        <property type="nucleotide sequence ID" value="XM_045114008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8Q4"/>
    <dbReference type="SMR" id="A0A8I6X8Q4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0154590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0154590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0154590"/>
    </dbReference>
    <dbReference type="GeneID" id="123430094"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0127370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0127370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0127370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0154590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0154590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0154590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025757">
        <property type="entry name" value="MIP1_Leuzipper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23054:SF55">
        <property type="entry name" value="ELECTRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23054">
        <property type="entry name" value="TERNARY COMPLEX FACTOR MIP1, LEUCINE-ZIPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14389">
        <property type="entry name" value="Lzipper-MIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ternary complex factor MIP1 leucine-zipper" evidence="3">
        <location>
            <begin position="79"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="DUF547" evidence="2">
        <location>
            <begin position="426"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0154590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="73090" checksum="AEC344A4438E4025" modified="2022-05-25" version="1">MREMEAQEKKGHRSLAKAKPVKPSYNYNAPSRHKRSKSDLEEKNAKDAPQTASNQPKLNGRNSNTHLQSETNKGIQPKSHTQDSLRKEIVQLERHLDDQQTVRGALEKALGPNPAPVTLSNDSPILQPTNQLIREVATLELEIKHLEQYLLTLYRKAFDQAPSLVVRPEEPAPAKPSMSSRSALMEETPKAKATVGRGGDAMLHYSCPPLSKRRNGTMEDCSPSTCPTKAMDSDHGLRSQSALSFRGVCSSRISPSEESLARALRSCHSQPFSFLEEGEAATTSGVVSLADYLGTNVADHIPETPNNLSEEMVRCMAGVYCKLADPPLVHHRASSSPTSSLSSTTSVVSPQYLGDMWSPNCRREATLDSRLINPFHVEGLKEFSGPYNTMVEVPSISRDRPRLREVEDLLQTYKLILHRLETVDLRRMANEEKLAFWINIHNALLMSAYLKHGIPQNHLKKTSLLVKAECKIAGRSINAAAIQGLVLGCSTHCPGQWLRTLLQPRMKSRGSKAGGQWQAFGIHRAEPLLRFALCSGSHSDPAVRVYTPKRLRQQLEAAKEEYIRATVGVRPPDHQHRRGRVVLPKLVEAYARDVGLSPERLLDAAQRCLPESVRAAVQRCRQQQQGTAASKAVVEWAPHRQSFRYLLARDLAFPHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBA2</accession>
    <name>A0A8I6XBA2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448183</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980928.1">
        <property type="nucleotide sequence ID" value="XM_045124993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBA2"/>
    <dbReference type="SMR" id="A0A8I6XBA2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0356120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0356120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0356120"/>
    </dbReference>
    <dbReference type="GeneID" id="123448183"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0297110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0297110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0297110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0356120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0356120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0356120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448183"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044590">
        <property type="entry name" value="CML48/49/50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46824">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46824:SF11">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0356120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="326" mass="33881" checksum="56EB43F628B49B6E" modified="2022-05-25" version="1">MAGYPPPPGSGYPYGAAGGYGAPPPSGQKPPKEGKTSSSSGPDPYHGAPPPQQPYGGGGGGGYGAPPSYGQKPPKEGKTSYSSGSDPYHGAPPPQQPYGGGGGGGYGQQPYGAQPPSSAAPYGGPPAAQPYAGGGAGGYGSPFAALVPSTFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSSQSFSLRTVHLLMYLFTNSNVRKIGPKEFTSVFYSLQNWRGIFERFDRDRSGRIDAAELRDALLDLGYSVSPTVLDLLVSKFDKTGGKNKAVEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFGYEAFMLTVLPFLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCH6</accession>
    <name>A0A8I6XCH6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="7">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123446523</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the CCR4-NOT complex, at least composed of CRR4 and CAF1 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044979032.1">
        <property type="nucleotide sequence ID" value="XM_045123097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCH6"/>
    <dbReference type="SMR" id="A0A8I6XCH6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341190"/>
    </dbReference>
    <dbReference type="GeneID" id="123446523"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0341190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446523"/>
    <dbReference type="OrthoDB" id="670902at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="299" mass="32903" checksum="68B8B3B51AC03573" modified="2022-05-25" version="1">MSPAAGMFPVFPPPPFCYTFPVQPPPYFHHAPMSPVWPSPAVPVRSVWAYNFNAESDSLRSAARRARYVAVNVHYPGVVHHAGGQDHNTLTAEQRYALVKANVDGLTPLQVGVALYGNDDGYLGAWEFNLRDFRPRADPHDEKSLAYLARRGLNVGALRDHGVRADMLCATLFESGLISPRRGPPRSWITYAGAYHVAYLVKIVTRGAALPEDVAGFDDAVRRYLGNQVYDVAWMAGDCPAMPLGLERIAAHLGFHLPLWSPQLAAAAGVRALQVFMHLKYGEFGGNVQMYRGLLEGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDK3</accession>
    <name>A0A8I6XDK3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDK3"/>
    <dbReference type="SMR" id="A0A8I6XDK3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0202130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="48"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25032" checksum="6C8365A35734A99C" modified="2022-05-25" version="1">MPTRFEKVLAHGTTMLAVVYTNESREVPFFLEQLKERWLDAAMDREKFLGLDLEYTADQRGVAVIQLCFARHVLIFQWASSDKHCPELMDFLRSGITFATVDIRNDKLKMRYSFGIEIPTGCLIDLQTVFRLRHVRTSMAHMAAALIDEEYGDMKTSFPKSQHKLWEKAPLDPINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEL0</accession>
    <name>A0A8I6XEL0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEL0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0366130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0366130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0366130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0366130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0366130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0366130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF31">
        <property type="entry name" value="OS06G0482850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0366130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="22684" checksum="A5D6E09D51481953" modified="2022-05-25" version="1">MDEAELVLAHVMVASITGNRLRVSPDEVAELLLSSLELAEGDLTEHAHHPEDFLIVFPSLATMRRLNGEHFISSPRSALSMRPWCKLAHADADELGYRVELELRGIPAHAWHLSTAEHVLGASCWIERLHPRTRCRADLAVSRLSGRAHDPAEIRRAAILAIIEQLPPRMPSEAPTIRTLSFPISIALTKVELMRAADTSQTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEY2</accession>
    <name>A0A8I6XEY2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEY2"/>
    <dbReference type="SMR" id="A0A8I6XEY2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF21">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035192740" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="11">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="12">
        <location>
            <begin position="167"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="52382" checksum="B9EC15980EF9459C" modified="2022-05-25" version="1">MRSHTMLGLLITLALWCPVITNNTRGTAAACITVERDALATFNASITDPGGRLRSWQGVNCCSSGGVSCSKKTGHFVKLDLGAYSLKGEISPSLATLNRLVHLNLSHSDFDGVPIPEFVGSFKMLRYLDPSHAGFGGTAPAQLGNLSRLVYLDLGSFGGPGITADNFHWVSKLTSLRYLDLSWSYLAASVDWLQAVNMLPSLQVLHLNDASLPATDLTSLSQVNFTSLKLLNLKSNDLNSSMPNWIDKLSALSELDMTSCGLSGMIPDDLGKLTSLKFILLGDNKLTGVIPTSANRLCNLVQISLSGNTLSEDIAEAGKSMSPSMKRLQVLELSGNKLTGNLSGWLEGMSGLRILDLSANSVSGVVPYGIGPVPHSLASVPYLSYLNLSYNDLSGQIPFDTLTCLFTLLGFAFGISTVFTTFICSAVARKAYFRFTDRALSKLWCTAVEMKLSINRMMPAGRDPSMATRSQDTITCYELEQPSAASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG66</accession>
    <name>A0A8I6XG66_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123427541</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967550.1">
        <property type="nucleotide sequence ID" value="XM_045111615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG66"/>
    <dbReference type="SMR" id="A0A8I6XG66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188820"/>
    </dbReference>
    <dbReference type="GeneID" id="123427541"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0156350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0156350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0156350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0188820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427541"/>
    <dbReference type="OMA" id="FLTEEFM"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044:SF57">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="471" mass="50850" checksum="4260F6E9D8D7F8D3" modified="2022-05-25" version="1">MAPAAMEVAPAPVAIVAVPFPAQGHLNQMLHLSLLLASRGLDVHYAAPAAHVRQARARVQGWDARAVGSLHFATLDIPRYATPPPDPDSPVAFPCHLQPLFHAFCDHAAGPLGRLLEELAASHRRVVVVHDNVVAFPALEASRLPNGVAYALQCVGQSFGAGFKDPSHRLVRALGLPVPSPESFLTEEFMGLVERQGGLGVPGAGLLLNSCRALEGEFIHAQAETLSLDGKRLFSIGPLNPLLEQDLDATKPALSVQPRHECMDWLDKQPPSSVLYLCFGTMSSLPGKQIEELAGALQSCEQRFIWVLRDADRADIFAEAGESRHAKLMSDFTKRTEGRGLVITGWAPQLEILAHGATAFFVSHCGWNSLLEGLSHGKPILAWPMHSDQPWNAGYVCGHLKAGMVMRSWEKSRETLPAKEIQEVIKRAMDSDQGIAVRSAAKALAEDVRAAVANGGSSWADMEDFIGCITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGF3</accession>
    <name>A0A8I6XGF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGF3"/>
    <dbReference type="SMR" id="A0A8I6XGF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0321450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0321450.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0321450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0386360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF128">
        <property type="entry name" value="OS03G0282100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="7">
        <location>
            <begin position="17"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="322" mass="36178" checksum="8205BEFFA3F5C722" modified="2022-05-25" version="1">MAATTEVATYANPWWVEVWVLTRRAFTNTWRTPELLVIRFGAVVVTGFILATFWRLDNTPKGVNERFGFLAIAMSTMYYTSADALPVFLIERYIFLRETAHNAYRRSSYTLSNAIVAFPPLVVLSVAFTGITFFGVGLAGGTEGFVFFALIVLASFWAGSGFVTFLSGAVPHVIIGYTVVVAVLAYFLLFSGFFVTRDRIPGYWIWFHYLSLIKYPYEAVMQNEFGAEPGKCFMRGVQMFDGTPMGKLPVATQVTVLNAMSKSMRIDFNSSSCITTGMDILAKQAVDQLGKWGCLWATLAWGFLFRILFYFTLLLGSRNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XH82</accession>
    <name>A0A8I6XH82_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Helicase-like transcription factor CHR28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123427954</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968034.1">
        <property type="nucleotide sequence ID" value="XM_045112099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XH82"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0199060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0199060"/>
    </dbReference>
    <dbReference type="GeneID" id="123427954"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0165370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0165370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0165370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0199060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0199060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0199060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427954"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000068">
        <property type="entry name" value="SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF3">
        <property type="entry name" value="OS04G0629300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="389"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="723"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="873"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0199060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1049" mass="115296" checksum="AF5DB96E8245EE79" modified="2022-05-25" version="1">MEIIDLCSDSEDDVKLCSKGKGITHLCSEDRKDNVVPIDGVDSSLLLLNTPFAKNNAQQPITLDDDDWISSNHASCSYRSPAGSSHRIYPLSSSSLMLYDSSYGKTKQEIDDDDDIYMYSVPPQSFPISHPSLSDEEDLTARFNYVGTSADDGTGMFSSATSVDWKPPFGSSQVKTDSDNDDVYAYEGPHSHRMFPPSMPSFNPVNGPEFSNGFSTQSHPNGEKRPLGCDERAIYEEALQHISQETKEEDLPEGVLSISLLKHQRIALAWMLSKENSSHCPGGILADDQGLGKTISTISLIQKERVQQSNFMSADSDSKNSVPLDLDDDDIVMAMDKKEPSDSLDHELCSSLSGSAFNNMAKNVKVEPRKKARVGSASISRSATRPSAGTLVVCPASILKQWASEIKAKVTESSRLSVLVYHGSSRTTKPTELAKYDVVVTTYTIVGQEVPKQDSDDDMEPNIDEKYGICPDFAARKKRKLSKQTKKKAIKKKKLSSSDADLGGGPLARVRWFRVVLDEAQTIKNHHTKSARACCGLKAKRRWCLSGTPMQNTIDDLYSYFRFLKYEPYSSFSLFRSMIKGPISRGSSQGYKKLQTVLKIILLRRTKETLLDGEPIIKVPPKTIELKKINFTQEERYFYLALEEGSREKFKEFAAAGTIKQNYANILVLLLRLRQACDHPYLLKDMSNKTNYTDPASIEMAKQLPREIVMNLLEKLEVRRPICMICAEHRNNGMESRTGPKEPPENAVITTCCHIFCYECAQESLSEEEVCPVCKQKLCSELLFSRPVLRLCISDELESYATAAGDSSAAAAAAADSSVVVAAAADSSAVVAAASAADSSAAAAAASAADSSAAAAAADESSSICEASYISSKIKGAVDTLKSIFNTHGPADSDTPKAIVFSQWTGMLNVLELSLNSNFINFRRLDGSMSLDDREEAVQEFKADPEVRVMLMSLKAGNLGLNMIAASHVIMLDPWWNPYAEDQAVDRAHRIGQTRPVTVTRFTVNGTVEDRILALQAKKREMVESAFGEKSGGISTRLTVEDLGYLFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHC6</accession>
    <name>A0A8I6XHC6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123444899</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977716.1">
        <property type="nucleotide sequence ID" value="XM_045121781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHC6"/>
    <dbReference type="SMR" id="A0A8I6XHC6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302080"/>
    </dbReference>
    <dbReference type="GeneID" id="123444899"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444899"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19125">
        <property type="entry name" value="AKR_AKR4C1-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000010">
        <property type="entry name" value="NADPH-dependent aldo-keto reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044498">
        <property type="entry name" value="AKR4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="7">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="311" mass="34263" checksum="6D8653D00F8058A3" modified="2022-05-25" version="1">MATHFVLNTGAKIPSVGLGTWQSDPGVVGEAVYAAVKAGYRHIDCARAYNNEKEVGLALKKLFDEGVVKREDLFITSKLWCGHHAPEDVPEALGDSLSDLQLEYLDLYLIHWPFKIKKGTSIGNPENFLPPDIPATWGAMEKLHDAGKARAIGVSNFSSKKLGDLLAVARVPPAVDQVECHPSWQQSKLHSFCQSAGVHLSAYSPLGSPGSTWMNGNVLKEPVVLSIAEKLGKTPAQVALRWNIQMGHSVLPKSVSEERIKQNLDVYDWSIPEDLLAKFSEIKQGRLLMGNFIVNKDSVYKTHDELWDGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHX6</accession>
    <name>A0A8I6XHX6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123426318</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044966064.1">
        <property type="nucleotide sequence ID" value="XM_045110129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHX6"/>
    <dbReference type="SMR" id="A0A8I6XHX6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0155570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0155570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0155570"/>
    </dbReference>
    <dbReference type="GeneID" id="123426318"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0128260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0128260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0128260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0155570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0155570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0155570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426318"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000357">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF1">
        <property type="entry name" value="OS07G0119000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="368"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="85"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="723"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0155570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="743" mass="80668" checksum="EC16880E09B43DE2" modified="2022-05-25" version="1">MPSWWKRSKSAFHLSATTASSSSSSAPVSPSRASTSRSQPRRAGSDDAGDLLLARPHRQPRQLTRQRKLRHVYDIDALLADLGIDVASSSPPPHARGRASASDAVGFGPPISRSSNAAEGVVAPPPRSASSPVLHPLPLPSPSPKPPAELETSEPAGVAEGGNERTSLQIPRVTGQTVQKFPEHNDFGSNGTRRPASSHHRKALHDKFQDKSSAETGNFRLNIPTKSAPTSGFSSPVGSPRRLSNADVSSTVASSYAPQAWSAPSIHTIDFLGASSPRTSPEKLIGASEPSPYSSTFRSPILMPRNTSAPPSPHPKLFPENQISRTEVNGSVSLHPLPLPPSAISPMQTSFSNQPAPKVEMPSVACQWQKGKLLGSGTFGCVYEATNRNTGALCAMKEVNIIPDDAKSVESLKQLEQEIKFLSQFKHENIVQYYGSDTIEDRFYIYLEYVHPGSINKYVKQHYGAITESVVRNFTRHILRGLAFLHGQKIMHRDIKGANLLVDINGVVKLADFGMAKHLSTAAPNLSLKGTPYWMAPEMVQATLAKDVGYDLAVDIWSLGCTIIEMFDGKPPWSDLEGPAAMFKVLHKDPPIPENLSQEAKDFLQCCFKRNPAERPTASELLDHPFIRNSSHYNKHGSIHAFAGIKVHDNGYGFRDKTTSKSEPYVKGRNTIGEPNSARPFESSTFRLTPLTIQGVTPNFAPQPFGLASNPGSFAVSTNPMHFPMANPQPSPLPRPNGKEVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI52</accession>
    <name>A0A8I6XI52_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123428338</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044968474.1">
        <property type="nucleotide sequence ID" value="XM_045112539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI52"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0209560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0209560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0209560"/>
    </dbReference>
    <dbReference type="GeneID" id="123428338"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0209560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0209560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0209560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428338"/>
    <dbReference type="OrthoDB" id="1927254at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF327">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="51"/>
            <end position="105"/>
        </location>
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        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
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        <location>
            <begin position="96"/>
            <end position="133"/>
        </location>
    </feature>
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        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
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        <source>
            <dbReference type="RuleBase" id="RU369094"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0209560.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="208" mass="22055" checksum="9F150A167A8C0A5C" modified="2022-05-25" version="1">MAPASASLLPAAGAKRPFAADSAVDAADEGQPLAQQSAVTKKHGESQQEKLECPRCSSSDTKFCYYNNYSTAQPRHYCRTCRRYWTHGGTLRKVPVGGACRRGSGSSNKRRRPSAEPHTTSSDSPQQDTLPPLPVFPFLTDGGPVFLPQFDLGLGGFPWTTPAATDNLYDGLAPWGGCDGALAPTGAWEDLGGLELTWPPPPPPAAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIC0</accession>
    <name>A0A8I6XIC0_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0435610"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0435610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="98" mass="10950" checksum="775599DCFC8EE1A1" modified="2022-05-25" version="1">MEQCRQGKSAKRIRNFGKRIGSEDWARGSRPRTRRLLADCSSCSRGESGSPRAGRGTDRESPLRELSTSMKQSTQNWYGQGESDCLIKTKHCDGPRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIP4</accession>
    <name>A0A8I6XIP4_HORVV</name>
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        <recommendedName>
            <fullName evidence="19">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123428519</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968676.1">
        <property type="nucleotide sequence ID" value="XM_045112741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIP4"/>
    <dbReference type="SMR" id="A0A8I6XIP4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215660"/>
    </dbReference>
    <dbReference type="GeneID" id="123428519"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0179250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0179250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0179250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0215660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428519"/>
    <dbReference type="OrthoDB" id="664066at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="318"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="395"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="716"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="753" mass="82190" checksum="13716230CE7903E9" modified="2022-05-25" version="1">MHCILRVFAHRSRHRASQRPPTGMASFSSATTSTCFLLLHLVIIFFHLPTPAVSLAFNCSRFTTEDLKDMKFEGDASFNTLYGSIPMNTNTYRVASRVSYSAAPLQLWDKMTGEVASFTTQFQFQIHITGDESSKGGMAFFLAGYPSSLPGNCTPDSLGLTNQSADAVPSGDSRFVSVKFDNFNNTIVADPDTSSYDHIGINVNSLISVSTQPLPSFTLNNTQTAQIEYDNVSSILAVTLWSDDADGQDQRSSSLSSKVDFKSVLPEQVSIGFSTNTLSGMDIFLNSWYFNSSLEPQQTPPPPPPPPPSSPPQSPPGLGSGVIVGAAAGATMFLVLLFAAAAALVVRRRRHQNMMETEEYYTDSEGEGEPMSEIEMGTGPRRFPYRELMEATKNFAAEEKLGQGGFGAVYQGYLRDPAGLAVAIKRLQSSIQGKKEYKSEVKVISRLRHRNLVQLIGWCHGHDQELLLVYELMPNRSLDIHLHSKQGTFLTWPMRMKILLELGSALLYLHEEWEQCVLHRDIKPSNVMLDESFGAKLGDFGLARLVDHAAGMQTMTAISGTPGYLDPECVNTGRASAESDVYSFGVVLLEVACGRRPMSVIAPNQHQQKNGGVFRLVEWAWGLYGRGAILEAIDERLNGDYDAAEVERVMVIGLWCAHPNPSARPSIRTAMATLQPKVDQAYQLPMLPSKMPVPVFEPFALANAVGMSSSSSHTFGVSSSTWSSMPPHTSGTASSDASTSTNSSKTSSSLLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJD4</accession>
    <name>A0A8I6XJD4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123448545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981424.1">
        <property type="nucleotide sequence ID" value="XM_045125489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJD4"/>
    <dbReference type="SMR" id="A0A8I6XJD4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370780"/>
    </dbReference>
    <dbReference type="GeneID" id="123448545"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0308580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0308580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0308580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0370780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448545"/>
    <dbReference type="OMA" id="WGLKYAV"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="540" mass="57959" checksum="23F8E8161C2D5BA8" modified="2022-05-25" version="1">MVGYGIQSMLKDGHKHLSGLDEAVLKNIGAGRELSAITRTSLGPNGMNKMVINHLDKLFITNDAATIVNELEVQHPAAKLLVLAARAQQEEIGDGANLTISFAGELLEKAEELIRMGLHPSEIIIGYTKAINKTLEILEDLVEKGSENMDVRNTEEVMLRMKSAVASKQFGQEDVLCPLVADACIQVCPKNPANFNVDNVRVAKLVGGGLHNSSVVRGMVLKNDAVGSIKKVEKAKIAVFAGGVDTSATETKGTVLIHSAEQLENYAKTEEAKVEELIKSVADSGAKVIVSGAAVGDMALHFCERYKLMVLKVSSKFELRRFCRTTGAIALLKLSRPNADELGYTDSVSVEEIGGARVTVVKNEEGGNSVATVVLRGSTDSILDDLERAVDDGVNTYKSMCRDSRIIPGAAATEIELAKRLKDFSLKETGLDQYAIAKFGESFEMVPRTLSENAGLGAMEIISSLYAEHAAGNMKVGIDLEGGACKDASIMKIWDLYVTKSFALKYSADAVCTVLRVDQIIMAKPAGGPRPPQQGAMDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJU2</accession>
    <name>A0A8I6XJU2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJU2"/>
    <dbReference type="SMR" id="A0A8I6XJU2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0190750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0190750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0190750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF909">
        <property type="entry name" value="OS11G0673900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="191"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="432"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="548"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="841" mass="95801" checksum="DA300A6CA7B7E029" modified="2022-05-25" version="1">MAAILETLLKSSVNKLQDVITDEAILILGVEEELTKLLRRVELIQCCIYDAEKRRTKEQAVNNWLGQLRDVIYDVDEILDVARCKGSKLLPDDHPSPSSGKSFACKGLSVSSCFCNIVPRHDVAVRIRSLNKKIECIANDKVFLTFNNSTQPIANGQTSKLIRSSNLVEPNLVGKEIIHASRKLVDLVLAHKEYKSCKIGIVGTGGVGKTTLAQKIYNDQKIKGSFMMHAWICVSQDYNEVILLKEVLRNVGVHNEQGETVSELQRKLAETIEGKSFFLVLDDVWHSNVWTDLLRPALHETTSGVILVTTRDDQITRRIGVQHTHRVDLMSAEVGWELLWKSMNIEEEKEVQYLRSTGIEIVHKCGHLPLAIKVTASALASRDLTENEWKNYLGKYAGSPSMLCDEIEGALYLSYDELPHHLKQCFLYCALYVEDYIIERDEVTWLWIAEGFIKEQQGQLLEDIAEEYYHELIHRNLLQPDISYFDMSHCKMHDLLGQLALNISREECFIGDVETLREGNMSKLRRITAVIKKDMLVLPRVDPVGVKVRTFLTVKGPWRSEDTLFKRFLLLRVLVLNYSLVQSIPDYIGKLIHLRLLNLDHTGISCLLESIGSLKNLQVLSLRWCNDLHNLPLAVTLLCSLRCLDLYGTKINEVPKGIGKLKFLTYVDGYRISDGSNDTVAQDGWKLEELSSLSQMRYLSLVKLERATHCSTSAVLTDKKHLKELVLEWTERGEGSYSEEDVSNAEKVFEQLIPPPNLEDLWIFSFFGQRYPTWFGTTYLSSLIYLKLENVRSCVHLPPIWQLPNMKYLRIEGAHAVTKVGSDYRVILYAMSCLLFLNLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKA0</accession>
    <name>A0A8I6XKA0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123439449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972095.1">
        <property type="nucleotide sequence ID" value="XM_045116160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKA0"/>
    <dbReference type="SMR" id="A0A8I6XKA0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234840"/>
    </dbReference>
    <dbReference type="GeneID" id="123439449"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0194890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0194890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0194890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0234840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439449"/>
    <dbReference type="OMA" id="CDGHSCP"/>
    <dbReference type="OrthoDB" id="775830at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF55">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035149993" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="83"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="454" mass="47788" checksum="FC18A974EAA55586" modified="2022-05-25" version="1">MAGRALLLIVFVLTARLCGCTGRVVGGGFSVEFIHRDSAKSPFHDPSLTPYDRVLAALRRSTARLYPGPGGAVSEVVSTPYEYLMYVNVGTPRTRMLALVDTGSDLVWFKCSNGTAGPPPGAPLSVAFDPSSSSSYGLLGCRSDSCHAIYGTSCDAKSFCRYRFSYADGSMTTGLLSTETFTFDDAPGGCVGCRDRPQLQVPRVNFGCATNVTGQASRLTGNVGLGAGSGSLVNQIGAATSLGRRFSYCLAPFYVNASSILSFGSRAAVTEPGAVTTPLIPSVVDAFYTILLASVKIGSSTIMPPKRSPVIVDSGTVLTYLDKGLLDLVVAELARSIKLPRKQSPERLLDLCYDVAGETEAWVKLFPEVTLEFGGGAVITLQARNAFVEAREATVCLAMSAVTDNRPVVATIGSIAQQNFHVGFDLDKGAMSFAAADCASSYSSLLPPSVYLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMN4</accession>
    <name>A0A8I6XMN4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447864</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980505.1">
        <property type="nucleotide sequence ID" value="XM_045124570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMN4"/>
    <dbReference type="SMR" id="A0A8I6XMN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345140"/>
    </dbReference>
    <dbReference type="GeneID" id="123447864"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0288120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0288120.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0288120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447864"/>
    <dbReference type="OMA" id="DHERQET"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146:SF2">
        <property type="entry name" value="OS11G0222000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE-LIKE PROTEIN CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035214115" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="456"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="774"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="793" mass="84166" checksum="23A2794E01F6BA61" modified="2022-05-25" version="1">MMRPPRGILLFLAMLVALLLVRPGAASTLATFAMAKAEETTIVCGLLPSAASPALFDLNCTAAGGDHERQETYPSTHPFSALAGGEDFLCAVGPSSARAGAVDMRWWDLSKNAGRSKRVYLGPPLRALASSGYRVCGVLSSGELHCWRWRGLAIPAGLRFVAVAVGKGFVCGIVDGQDAPIRCFGNKTEDIEAVRAAPQGGSYDVVVACGRHACALSMGGGLSCWGSGAPALDGENVTAGYAALALGESGVCGLRTNGTIRCFGHGVAAPPEPLAGLQYIDVQAQGCAFCGVLLANYSLVCWGGHEFNATNRLVFDRVLPGPCVPMSSCQCGVLPGSANLCAGGRCICVDCAFELNVAFPKAANPGEASSSSSRRRRKIIIGVAVGAAALLVLVVALQFALLLWCRRRRRRNNSPDELAAMQSLMPPRLGSSRSKGPGSVVEHFTLDTLRAATEGFDDSRRIGSGSFGSVYRGTLPDGREVAIKRAEDHAKSSSSAARPARRRDRETAFNSELIALARANHKNIVCLLGCCAESGERVLVYEFMMNGTLHDQLHDRSPMAAPVLSWRGRLTIALGAARGIEYMHVYAVPPIIHRDIKSANILLDDSWTAKIADFGLSSVLDPAGDCEDNGGDDPQQQQPQRTVYTGGTVGYMDPEYYRLQHLTDKSDVYSFGVVLLELMSGCRVVQRYAESVTPKNVVDFTVPHILADDVARVLDPRLPAPTAHEGEALAYVGYLAADCVGPVGCERPSMTEVVDALERALAACSTAPVSRSGTARRVLSRSGTDQFDLTDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPM7</accession>
    <name>A0A8I6XPM7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0189920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0189920.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0189920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.40">
        <property type="entry name" value="ENT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033485">
        <property type="entry name" value="EMSY-LIKE_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005491">
        <property type="entry name" value="ENT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036142">
        <property type="entry name" value="ENT_dom-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432:SF35">
        <property type="entry name" value="OS01G0611200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03735">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01191">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158639">
        <property type="entry name" value="ENT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51138">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENT" evidence="4">
        <location>
            <begin position="339"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="405" mass="42862" checksum="AFF7F19A43314124" modified="2022-05-25" version="1">MRITLGMSLEAWVTTDAVAPPMAMGAWRAGEVVWGNGHSYVMRWFDGGPDSGRILRKFVRPLPDPAVQLPADLGAGDNVEVLDGSLWKWAEVVAAAADGHGGLFEVKIVGSSQVLTAERGALRPRQVYGEKGWVVLHKGNKIPAESVVPSRPIAGKNINGKATNGGSKFGAAPHAAVMLGKSTKRSSCTVTVDAGLVRDAKRPQCYGDANPCYLMKKRPQCDLVGGAARDSSSSDSDDDSSSTSSKSDSSSSADSSDDSSSTNGSSSNGGGVRAAVPTAGEQCQENRQASPPNSEGEGQDSNDDGRASASATMQHRLAPADEEEAVTMRRNRQAEEGEHGHGVHGLELEAYVSVMKAFHATGPLSWAKEELLTGLRLQLHVSGDEHLQVIRRLNGNNKTRKRLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRB9</accession>
    <name>A0A8I6XRB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRB9"/>
    <dbReference type="SMR" id="A0A8I6XRB9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0553440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20953" checksum="F5EDAAE15E6D872E" modified="2022-05-25" version="1">MLTRFGEVLAHGKTKLDVVYTNESREMPYFLEQLKERWLDAAMDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRSGITFATVDITNDKLKMRTSMAHMAVALIDEEYGDMKTNFPKSQHKLWEKAPLDRINVEYAAKDAYVSYELYRKIRVVDYGQRHLE</sequence>
</entry>
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    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the EXORDIUM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948195.1">
        <property type="nucleotide sequence ID" value="XM_045092260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XT76"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429970.1">
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    </dbReference>
    <dbReference type="GeneID" id="123397722"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0357310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0357310.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0357310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0429970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0429970.1.CDS1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006766">
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        <property type="entry name" value="OS10G0376400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN EXORDIUM-LIKE 5"/>
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    </dbReference>
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        <location>
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            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035234188" evidence="6">
        <location>
            <begin position="30"/>
            <end position="318"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="318" mass="32583" checksum="80F3D25374A911A1" modified="2022-05-25" version="1">MTTTKPHAACSLLLALLVVGLTRPPPCASSPYNPPPPAMTYHHGGVLDGTVPVSVLYYGAFSPRHKAVLADFLHSLSPRSRPHGAFGAPSSSSSVAQWWETVDRYVQRAGRERTRVMLTNQVSDEGCSLGKHLSRLQVEQLAARLGVAPGGVAVVLTAADVAVDGFCGSSCGLHGSLAPGGAVHVWVGNAAVQCPGRCAWPFHAAPGRRHGSGGEAPLRAPNGDAGVDGMVINLAALLAGAVTNPYGRGYFQGEAGAPVEVGGGCPGAYGRGAYPGYPGAVKLDAATGGGYNVVGRNGRRYLVPALVDPANYSCLIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="1" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR32011:SF4">
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        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="291" mass="31004" checksum="9368171D34AB716A" modified="2022-05-25" version="1">MAFQPRRTVPPAIGPIGARGEVRAVAMVDVEHRMAGMAPAAHAAGLRRLSTRAAADPSSASASPRHGLYSFQGVASSVLSHLRASGVAILLGLSETELARAEAEMGFTFPPDLRAVLALGLPSGPGFPDWRSRAGLRAAFDLPVAAASLQIARGALWPRCWGKRPADPERSRRLARSVIRRAPLLVPLFDRCYLPCSPSLAGNPVFFVTDDPVLCCGLNVVHFFTRESSFQPMDISSQFAAMPSSGTSTPCTRRSLDAACGGQAPRWIEFWSDAASNISRARTSEEHASSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0267350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28827" checksum="B9F056AAF8B9E9AA" modified="2022-05-25" version="1">MPGHPSRQVVHLTRCIDVSGHTAMLVRVMSVCGYRWYPEYTVEEQYQDFNQSQYICTVRVFPDYPGAEEPIHWSYGLGVTVDIAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTAEMLENRDSDARALRMELYATRARVWTALTQLAPVVQTGYRDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGQRPHLCPYGSQGSQAGVFPDPQVELPGHGGNLYEMFYVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="10 11">
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        <lineage>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044984760.1">
        <property type="nucleotide sequence ID" value="XM_045128825.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6XWG2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510280"/>
    </dbReference>
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        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
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        <property type="entry name" value="DUF7028"/>
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        <property type="entry name" value="IDM1_C"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR013083">
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    <dbReference type="PANTHER" id="PTHR46309">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1029"/>
            <end position="1074"/>
        </location>
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        <location>
            <begin position="1165"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="392"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="530"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="812"/>
            <end position="834"/>
        </location>
    </feature>
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            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
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        <location>
            <begin position="405"/>
            <end position="418"/>
        </location>
    </feature>
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        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
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        </location>
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            <begin position="694"/>
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    </feature>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51186"/>
        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510280.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1350" mass="147119" checksum="9F10A34AB0578F75" modified="2022-05-25" version="1">MRHNGLERSPMPGIEDALMKDSPKASSLEVENPSLADFDHLGGGPTLAGKKRKDVPGGENGGVTKRVLRSDTMKLHVDAEPEGEAAAAAVDVSGADSLEKTDCGEAIAGDSLDKTGCGEAIAGDSLEKTNCEAVAGDSSEEANCEAIAEEGGSGLQTVDACEGDDEHMDEVPVKVDDAIEVPDKCYEDNVESLGVAEENGLDSQQSNSESDDKREKADEKIHSATQEEENVSAAATTGGCTDDSQENKAANGPCQGEVIDPSTAANDDELGIGVSTKSPTSASEPVEQDDTVVRTEGMVLHSGDQGVEKHRHNDDVPIDTGTSLIENGRCVADNHITLTDCTNQKGGLGSPVHRTKGFSTPDIVFIRRRSLTRNSCEMKQVKPEEEEVQFERRVTRSSTVRQREASGSSCKSTANAASMESKGRKEDVVHYRRKVSNTASLKADHTEPAKRGTSTKNNTLNGKVTAQRISSVTGNDYPANITENKASAAEIKINSKPQPSTRSGGIAKATTKDAVSLVDQNVCSSAFTEKNDTELTDSEGVRSENKTAVPKSPLSVGAKIVASKKRMLESGLDKTAGGSPVEMPSMKKTRSTSHTELDQSKKSSGKMATEKHSSSDKKDILTKQQHHSRAAELSRSVNPSNKDASKLSHNGSDVDGTDINITHKRNCRGRRERPPVLIKQEYSSESEEVIVVRKDRRKRKGPMRKQRSGSRPDHSSGSPKASNSKGNRKTKYGSKEKSGNPGEPTCALSEKRKISEQIKAILLDAGWKIDLRPRNGRDYLDSVYIPPTGKGSYWSITKAYAIYQSMQSEQKDEAASENLSKKSPGSPGKIHASTVSSLPEEILSKLKRVVVNKRKTKIELQKSKKRKHGLLKNSKNLKGRPKEKKKISKERKKRGGCALLARGSNQEAGSSNGFAPYEWKRTVFSWLIDLDVLSVNARLKCMDESRSKVLLEGLITRDGINCSCCSKVVTVLEFVAHGGGQLSKPYRNILVDGLDNDLLHCLIIAWDKQSDSERQAFFPVSTEGDDPNDDTCGICGDGGNLICCDGCPSTFHMSCLELEELPSDDWRCTNCSCKFCHEHLNHDVPDNAEIDPLHSCSQCEKKYHPSCSPETEKLSSVSSQAGNHFCQQSCRLLFEELQNLLAVEKDLGPEYACRIIQCIHEDAPETVLDLDGRVECNSKIAVALSLMDECFLPIVDQRTGINLIRNVVYNCGSNFLRLDFRGFYIFILERGDEIVSAASVRIHGTKLAEMPFIGTRHMYRRQGMCRRLLDGIEMILSSLKVEKLIIPAINELVDTWTSKFGFSPLEVSDKQEVKSINMLVFPGTGLLQKSLLNKQASPQEHPGSEGGQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y108</accession>
    <name>A0A8I6Y108_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y108"/>
    <dbReference type="SMR" id="A0A8I6Y108"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0288170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0288170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0288170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0288170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0288170"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0288170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="22638" checksum="FA162956A1C5681C" modified="2022-05-25" version="1">MEAIVKARKEANEVRKMARNQDAAAEERRVAAEERKVALEERKVGMEERAKLLEMEKHLFFLDTSLFNDAQKEYVNLAREEVLIQKRVLIRGMGGGGLGAMGGGGLGTMGGMGGYEAMGCMGAPPAAMGGMGGFGAPSDAMTAMGGMSFASLMGGMGAPPAAMGGMSAPPAVVEDLANTVGASCDAVRDEVREEDSSTEAEESSSKDEDEDEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y124</accession>
    <name>A0A8I6Y124_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroorotase, mitochondrial</fullName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123440245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
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</entry>
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        <submittedName>
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        </submittedName>
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        <name evidence="3" type="primary">LOC123449731</name>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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            <end position="41"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="236" mass="24952" checksum="33B97A01EE37D520" modified="2022-05-25" version="1">MGTMTMASSSLVLRPRASPLSPRRAPTRRAALPAPQATAAETPREGLSVVVGEGETRTPTTRLYSLAPYPLLLAALLPGAEPITAAFEPFVELVRTFSLPDWLVHWGHPGNMAVVLFAMGGYGTYLGFRIKLSDDPEEKAKAKDLHPKLLGGMFFFFALGATGGVTALLTSGKPIFESPHAVTGVIGLALLTVQSLLPTLFEGNPGLRGAHGLLGSSIMTLFLFHAAFGLQLGLSF</sequence>
</entry>
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    <name>A0A8I6Y579_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="5" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045127437.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0417550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0417550"/>
    </dbReference>
    <dbReference type="GeneID" id="123450040"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0347480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0347480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0347480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0417550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0417550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0417550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450040"/>
    <dbReference type="OrthoDB" id="620208at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Polyubiquitin-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
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    </dbReference>
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        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="159"/>
            <end position="245"/>
        </location>
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        <location>
            <begin position="245"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0417550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="396" mass="44623" checksum="4ACF0EA540F77889" modified="2022-05-25" version="1">MADGGPEAAPPLEFPSAGWRGLYDRLQGLLPRVEALAADRARLEEAVRLSDAREKSLHARLLQADASRERWKAAYAELPPGTNPKLAELQERDLVDSQSGKEALPDADNSQLKETSQKRRKLINDKTNKKKRTKLTNEETGQNKRTKLTNEMHLERCTMQIFVKINCSIFRKKRKTHCLKTITLEVMGSNTIYDVKDKIQEKEGIPAGEQRLMFGSELLVDSCSLEDYSIEEESTLTLDLVPRGMHIFIRTRSGKTMTIGVEGEDSLYSVKAKFFDETGIPPSRQRLFFAGKQLEDGRTLADYDVQNESTLHVVLRGITRQSGEMRISVKTVTGKKVIEHAMKRRQAIDNIKAWIYSELCILPEEQQLSGEDGAPLAEVIRSSCTVVLQIRPPGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6U4</accession>
    <name>A0A8I6Y6U4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF985 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123401251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950935.1">
        <property type="nucleotide sequence ID" value="XM_045095000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6U4"/>
    <dbReference type="SMR" id="A0A8I6Y6U4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611460"/>
    </dbReference>
    <dbReference type="GeneID" id="123401251"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0507050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0507050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0507050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0611460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401251"/>
    <dbReference type="OMA" id="HWHRIDA"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
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        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387:SF3">
        <property type="entry name" value="DUF985 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF985" evidence="1">
        <location>
            <begin position="10"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="21498" checksum="38D311668C6E463D" modified="2022-05-25" version="1">MGSCEMTATEVAALLDLKPHPEGGYYAETFRDSSVSLTTAQLPPQYKVGRAVSTAIYFLLPAGSVSRLHRIPCAETWHFYKGEPLTVFELHDDGHIDLTVIGPHLDAGQRPQYTVPPNVWFGSFPTLDVESFASDGSHLVNSRKRDPEKHYSLVGCTCAPGFEYEDFKMASFDDVKTIAPKAEPFLNYLIPSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y713</accession>
    <name>A0A8I6Y713_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123395940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044946898.1">
        <property type="nucleotide sequence ID" value="XM_045090963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y713"/>
    <dbReference type="SMR" id="A0A8I6Y713"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432950"/>
    </dbReference>
    <dbReference type="GeneID" id="123395940"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0359720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0359720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0359720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395940"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010349">
        <property type="term" value="F:L-galactose dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19163">
        <property type="entry name" value="AKR_galDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000011">
        <property type="entry name" value="Aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044479">
        <property type="entry name" value="LGALDH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="316" mass="34277" checksum="28EAAF96CC35A12B" modified="2022-05-25" version="1">MELRTLGGTGLRVSPVGFGASPLGNVFGDVPRDVARATVRRALDLGINFFDTSPYYGGTVSESVLGDCLRFAGVPRDSFVVATKCGRYKEGFDFSAARVTRSVDESLARLGLDYVDILHCHDIEFTNLDQIVNETIPALQKIKESGKARFIGITGLPLSIFTYVLDRVPPCSVDLVLSYCHYGINDTALVDLLPYLKSKGVGVITASPLAMGLLTDNGPPEWHPAPEELKLACRTAADHCRKKGKHITKLAMKYSLMNNEISTVLVGMNSPEQVEENVAAAVELSTSGIDEELLHEVEAILEPVKNLTWPSGIQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7B9</accession>
    <name>A0A8I6Y7B9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7B9"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28876" checksum="BBFEC0DF43530F51" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLARVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGAYFDSSMEDPLLQSTVEMLENRDKDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8Z7</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC123401507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951266.1">
        <property type="nucleotide sequence ID" value="XM_045095331.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="123401507"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0629340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0629340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0629340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401507"/>
    <dbReference type="OrthoDB" id="994207at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33344">
        <property type="entry name" value="OS02G0761600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0629340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="16216" checksum="851394C7E434BEF8" modified="2022-05-25" version="1">MTAASRLSYKNATVALCAINVLAVALLLRSHFSSWPRIAGGHRFDSDQLRYIWESEELRRAMEPVDLIRRVKEIEQEAYGEPGMSTAQEGTAAVDLSKRLKDLRQGNDGSSQRALEEWRKRKMERARQRAIGKDGMIPGTKTR</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541270.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="48"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25188" checksum="F86E333B7FA84361" modified="2022-05-25" version="1">MPTRFEKVLAHGTTMLDVVYTNESREMPFFLEQLKERWLDAAMDHEKFLGLDLEYTADQRGVVVIQLCFAHHVLIFQWASSDKHCPELMDFLRSGITFATVDIRNDKLNMRYIFGIEIPTGCLIDLQTVFRLRHVRTSMAHMAIALIDEEYGDMKTSFPKSQHKLWEKAPLDRINIEYATKDAYVSYELYCKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y962</accession>
    <name>A0A8I6Y962_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ADP-ribosylation factor-like protein 8B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123449413</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
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            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in lysosome motility. May play a role in chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982567.1">
        <property type="nucleotide sequence ID" value="XM_045126632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y962"/>
    <dbReference type="SMR" id="A0A8I6Y962"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398980"/>
    </dbReference>
    <dbReference type="GeneID" id="123449413"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0331930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0331930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0398980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449413"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
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    <keyword evidence="9 13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
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    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <position position="51"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
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        </ligand>
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            <dbReference type="RuleBase" id="RU003925"/>
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        </source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20711" checksum="B7AA4A95662A9C89" modified="2022-05-25" version="1">MGFWEAFLNWLRSLFFKQEMELSLIGLQNAGKTSLVNVIATGGFSEDMIPTVGFNMRKVTKGNVTIKLWDLGGQPRFRSMWERYCRAVSAIVYVVDAADRENMAIAKSELHDLLSKPSLTGIPLLVIGNKIDKPEAFPKQSFTEVMGLKAMTDREVACFMISCKNSTNIDSVIDWLVKHSKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6Y9M2_HORVV</name>
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            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="83"/>
        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="236" mass="27720" checksum="04C22F0A2CDE27DD" modified="2022-05-25" version="1">MVSWSEGSRSSWDDDSVTSQLPRTIPCYELSGIAHELSSRCMHLEPCLRQVAFQSTDTGWRFFACAKEKAEEKCCYLEWIDPEWSVSMQFCIGQLWSMHDKENKDKIRDNLKLGEENFKIVGEKRRMEEELRIFKLDFAKMVSNKEEAISQLGSARLAISDLKEELEKKKLADHSSTNLHHVLRAKAEKERDQLVLERDQMKEEKKKLEHIISDMMKQNHGYKDKVKKLKEICDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YC26</accession>
    <name>A0A8I6YC26_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123413511</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044962380.1">
        <property type="nucleotide sequence ID" value="XM_045106445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YC26"/>
    <dbReference type="SMR" id="A0A8I6YC26"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654930"/>
    </dbReference>
    <dbReference type="GeneID" id="123413511"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0543420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0543420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0543420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413511"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000008">
        <property type="entry name" value="MADS-box transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </citation>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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            <end position="66"/>
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        <location>
            <begin position="81"/>
            <end position="328"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="360" mass="42488" checksum="4EFA0A900533AF11" modified="2022-05-25" version="1">MDLLQRVLNFKGTPPWLNATALTALTDHWRPEMHSFHLPLGEMSVTLEDITMITGLPIEGRALTGKRYFRKCSNDASPTVVERYARAYLWNLLTQVVFLNGTGDTTSWMFLDPLCVWDVKWSWGSAALAFLYRQLDGACMRSKPTSTLGGFVWALQVWMWERIPVGRNLSLPPEEPWKYPFDGDEERYPTIAHTWANAQWTSIAPMGRYKAYISELDMLTYEQDNWRPYTILRRFPLRNMCLRDQHLWRVWCPMVCFFAVEWHLPYRVSMQFGVQQRSPPDHVETSVVLHKTNRQNNKSVINWEEHHIMWVDMWNAQRNTRVETDQTPDSDKAAYLRHLEWMRKEYKIIHKGARHDGVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKU9</accession>
    <name>A0A8I6YKU9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP synthase subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123418199</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the chains of the nonenzymatic membrane component (F0) of mitochondrial ATPase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962898.1">
        <property type="nucleotide sequence ID" value="XM_045106963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKU9"/>
    <dbReference type="SMR" id="A0A8I6YKU9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0754040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0754040.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0754040"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0760970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0760970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0760970"/>
    </dbReference>
    <dbReference type="GeneID" id="123418199"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0754040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0754040.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0754040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0760970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0760970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0760970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123416997"/>
    <dbReference type="KEGG" id="hvg:123418199"/>
    <dbReference type="OrthoDB" id="1932197at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000005">
        <property type="entry name" value="ATP synthase subunit 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF38">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0138">CF(0)</keyword>
    <keyword evidence="11 19" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 19" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0691">RNA editing</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="20">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0760970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="12176" checksum="DB51B8E327D47696" modified="2022-05-25" version="1">MEVQAQVLRIINKKSKKEQRRKNVTRKVFSRLEMLEGAKSIGAGAATIALAGAAVGIGNVLSSLIHSVARNPSLAKQSFGYAILGFALTEAIALFAPMMAFLISFVFRSHKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRB6</accession>
    <name>A0A8I6YRB6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123407187</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Ungrouped subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956200.1">
        <property type="nucleotide sequence ID" value="XM_045100265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRB6"/>
    <dbReference type="SMR" id="A0A8I6YRB6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0647310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647310"/>
    </dbReference>
    <dbReference type="GeneID" id="123407187"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0537210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0537210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0537210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0647310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0647310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407187"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000036">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF30">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="68"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="645"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="686"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="525"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="153"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="903" mass="99438" checksum="987DAC9F1AC4CEC2" modified="2022-05-25" version="1">MASGARNGGLGSRTAAKMDRQQSSTATPKAPAGKPRLSAASGAYRRTSSGPLPAAGGRNSSESGVSSRVRVAVRLRPRNAEEQAADADFADCVELQPELKRLKLRKNNWESDTFEFDEVLTEFSSQKRVYEVVAKPVVESVLEGYNGTVMAYGQTGTGKTFTLGRLGDEDTAARGIMVRAMEDILADITPETDTVSLSYLQLYMEMIQDLLDPVNDNIAIVEDPRTGDVSLPGATVVEVRDQKSFMDLLRIGEAHRVAANTKLNTESSRSHAILMVNVRRAVKGRSEMNVSMSAENGHSSSMMDSLRPPIVRKSKLVVVDLAGSERIDKSGSEGHTLEEAKSINLSLSSLGKCINALAESSAHVPVRDSKLTRLLKDSFGGTARTSLVVTIGPSPRHRGETTSTIMFGQRAMKVQNMVKLKEEFDYKSLCRRLDIELDKLIAENERQRKYFDDEIERITAEAQYRVAEAEKECKISLENEKMKYHQEYLDSIKILEEKWKVHQQSPKKQIKEPEPTSNGDVQDLLQNEKMLRQSAEDESNDLKNQLSHWKKMEATATAEVVRLRRMLDTEASQKDKLEEEIAVLKSQLMQLSLDADETRMSLDTGDGPGKIFPGLDSLMSHSRNSQPREQSNGPKAPVAKLFEQVGLQKILSLLESEEPDVRVHAVKVVANLAAEEANQEKIVEAGGLTSLLMLLRSSEDETIRRVAAGAIANLAMNETNQDLIMAQGGVTLLSMTASDAEDPQTLRMVAGAIANLCGNDKLQTRLRGEGGIKALLGMVKCGHPDVLAQIARGIANFAKCESRAATQGNKVGRSLLVDDGALPWIVKNANNEAAPIRRHIELALCHLAQHEVNAKDIVSEGALWELVRISRDCSREDIRMLAYRTLTSSPTLQSEMRRLRIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YTW6</accession>
    <name>A0A8I6YTW6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YTW6"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0575230"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.6HG0476970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0575230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0575230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0575230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0575230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="96" mass="11095" checksum="02E4788E4EB82D45" modified="2022-05-25" version="1">MEKGRDEDNAEAREEERLANLKARNSEDFARFGSFRFADAKEISRGKANQRQTKRKKSTVEKPTVEVTRVLRSQTTRDVDNMPSDNIEEHEGHEGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YW73</accession>
    <name>A0A8I6YW73_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YW73"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0606720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0606720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0606720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0606720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0606720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0606720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="150"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0606720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23254" checksum="1F1BC5A4A0F65727" modified="2022-05-25" version="1">MSLPCSDQSIRPRKMQSASLPRGVEAVRCWCGDVCKVKEVTDFSDWLGMKFFMCANYESDPPESISAYVRPPSPPPLCMYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYCVEQRAFLDEMKRKNQEEKLRLEEVYRQREQAREADRERKRERARAAKAAEEAGDGKGKYPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXP1</accession>
    <name>A0A8I6YXP1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXP1"/>
    <dbReference type="SMR" id="A0A8I6YXP1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0718490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15692" checksum="3A16901657EB6534" modified="2022-05-25" version="1">MVLVSASVGPPPVYDSDVEVCEPEIPTHIKILVEKLNLVEKENKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDVMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZG5</accession>
    <name>A0A8I6YZG5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleosome assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123413544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May modulate chromatin structure by regulation of nucleosome assembly/disassembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962411.1">
        <property type="nucleotide sequence ID" value="XM_045106476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZG5"/>
    <dbReference type="SMR" id="A0A8I6YZG5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650920"/>
    </dbReference>
    <dbReference type="GeneID" id="123413544"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413544"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Nucleosome assembly protein11"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650920.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="42809" checksum="0E0B9A87CA41F290" modified="2022-05-25" version="1">MGGEKENIDLSDLNISLPPAAAALSAEDRVDLVNALKDKMQSLAGLHADALESLSPNVRTRVEFLRGIQSQHDEIEAKFFEERAALEAKYQKLYEPLYAKRYDIVNGVVEVDGVVKEPTTENAAEGGDSDAKGIPDFWLTALKTNEVLTEEIQERDEPVLKYLKDIKWSRIDDPKGFKLEFFFDTNPFFKNSVLTKTYHMVDEDDPILEKAIGTEIEWYPGKNVTQKILKKKPKKGSKNTKPITKTEECESFFNFFSPPQVPEDDEDIDEDAADELQGQMEHDYDIGSTIRDKIIPHAVSWFTGEAVQAEDFDDMEDGDEDDDDEDDDEEDDEDEDEDEDEDEDEEELSKPTKKVAGKPKGPAKGGAQGNAEQPADCKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZS7</accession>
    <name>A0A8I6YZS7_HORVV</name>
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            <fullName evidence="14">Subtilisin-like protease</fullName>
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        <name evidence="12" type="primary">LOC123412707</name>
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    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
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        </lineage>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961580.1">
        <property type="nucleotide sequence ID" value="XM_045105645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZS7"/>
    <dbReference type="SMR" id="A0A8I6YZS7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654290"/>
    </dbReference>
    <dbReference type="GeneID" id="123412707"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542820"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412707"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035305825" description="Subtilisin-like protease" evidence="8">
        <location>
            <begin position="27"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="29"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="135"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="630"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="739" mass="78680" checksum="2588C0BA7B31B8DC" modified="2022-05-25" version="1">MDSRTAFRGALLLLVTLLPLSASASSKLYIVYMGDKKHDDPSVVTASHHDMLTSVFGSKDEALRSIVYSYKHGFSGFAAMLTKSQAETIAKFPEVVTVKPNTFHETHTTRSWDFLGLDHNQPGQQPGLLKKAKYGEDVIVGVIDTGIWPESRSFDDNGYSPVPARWKGKCQTGDDFNATSCNRKIIGARWYGRGISDEVLKKNYKSPRDIEGHGTHVASTIAGGEVQGVSYGGLGMGVARGGAPRARLSIYKACWLGGGCPVAAVLAAIDDAIHDGVDVLSLSLGGAGTEFPGTLHAVQRGISVVFAGGNDGPVPQTVANALPWVTTVAASTIDRSFPTLISLGNKEKLVGQSLHNNASLISGDFKGLVYAGSCDTESSLALSNVTGKIVLCYQPMAGRSMPPRQALPIAIKLTAMAGAKGLIFAQYTTNLPDILTLCKGVMPCVVVDFEIAQRIASYWSWDKGNAVVKVSPAMTVVGKGVLSPRVASFSSRGPSMLFPNILKPDIAAPGVGILAAWDGSYQFGSGTSMACPHVSAVTALLKSVHPDWSPAMIKSAIVTTASVTDRFGMPIQAEAVPRKLADPFDFGGGHINPDRAVDPGLVYDMDAREYNKFFNCTLGYLDGCESYYLNLNLPSIAVPDLKDHVMLRRTVTNVGPTEATYHLLVEAPAGIDVSVEPSVISFTKGSSRSVTFMVTFTTRQRVQGGYTFGSLTWSNGSTHSVKIPVAVRTVIQDFVADTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z0M8</accession>
    <name>A0A8I6Z0M8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123412878</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044961774.1">
        <property type="nucleotide sequence ID" value="XM_045105839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0M8"/>
    <dbReference type="SMR" id="A0A8I6Z0M8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742900"/>
    </dbReference>
    <dbReference type="GeneID" id="123412878"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0615980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0615980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0615980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0742900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412878"/>
    <dbReference type="OMA" id="MKANNED"/>
    <dbReference type="OrthoDB" id="605778at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000522">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035270980" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="29"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="344"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742900.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="698" mass="76272" checksum="DF89069153AAA259" modified="2022-05-25" version="1">MGSRRHPTSTPVVLVLLLLHLYYAPRWAFSLSFSLNFSGPGAGSSVDLDGDASIRPPRLELTRNLLSSVGRASYAQKVPLWNGATGEMASFTTNFSFQITPLKPVSGEGMAFFLGHFPSEIPPQSAGGSLGLLPALINGTGPTRIVAVEFDTLDNLYYGGVGPNHVAIDVNSMISTASTPTTSTPGKNLTSPYVMQAYVRYHNVSKMLAVDLLIDDALYQVSTTVDLSKELPEEVAVGFSAATADSSQLHRILSWSFSSTLPPLPIRNNNKKKLVMILSSVLVPFLSLLVCVAVVLCRRHKKMKANNEDNQERYAHRADLERGVAAGGPRRYTFNELVAATKHFAEEEKLGRGGFGSVYRGHLTLSSAAAGRRAVAVKMLSTESSAQGRKEFEAEVRIISRLKHRNLVQLLGWCDSRKGLLLVYELVSEGSLDRHLYSKGRCLTWPQRYKIILGLGSALHYLHGEWEQCIVHGDIKPSNIMLDSSLNTKLGDFGLARLADHGTGLMQTTKAVLGTVGYIDPEFVNTRRPCTESDVYSFGVVLLEIVAGRRPVMETAGKSFTLVRWVWSLYGRNAIIDAADERLRGDEADERWMGRALVVGLWCAHPDRSERPSIAQAMHVLQSDEVRLPALQLHMYRATPDPATYAGPYEAFSIDCSTSSSGCVRSSLVNTGDTTHSSDSSSTALLRNSKDLLPICKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z7Q4</accession>
    <name>A0A8I6Z7Q4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z7Q4"/>
    <dbReference type="SMR" id="A0A8I6Z7Q4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0546790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0546790.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0546790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0658870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF24">
        <property type="entry name" value="TTF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="90" mass="10518" checksum="F64948E9B91C4350" modified="2022-05-25" version="1">MEIFEFVREADCYPNISIAYRILFTMPVTVASAERSFSKRKLLKNYLRSTMSQERLNGLATLCIEKKMLDEIDIDTIISDFASKNVRRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZG09</accession>
    <name>A0A8I6ZG09_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZG09"/>
    <dbReference type="SMR" id="A0A8I6ZG09"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0751280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000362">
        <property type="entry name" value="Ser/arg-rich protein kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="142"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="255" mass="28273" checksum="7D38402FFB7C0056" modified="2022-05-25" version="1">MQSKLGWGHFSTAWLAWDTAHSRYVALKVQKSKQHYSEAAMDEIKILRQIADGDLEDSRCVVKLLDHFKHTSPNGSHVCMVFEFLGDNMLTLIKYTDYRGIPLPMVKEICPHVLIGLDYLHRELSIIHTDLKPENIFLVSTIDPSNDPRKSGVPLVPPAARVTEPPPRAPARPSTSSDVTRNQQKKIRKKAKHVVASTSEGNGAVAFADIDESDDKGDLNTANEGSPSQDGDRREEEGIGGATKGLGIGWRWKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0Q0</accession>
    <name>A0A8I7B0Q0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0Q0"/>
    <dbReference type="SMR" id="A0A8I7B0Q0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0023250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0023250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0023250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0029470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029470"/>
    </dbReference>
    <dbReference type="OMA" id="TRVARCK"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309:SF9">
        <property type="entry name" value="PEROXISOMAL FATTY ACID BETA-OXIDATION MULTIFUNCTIONAL PROTEIN MFP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="214" mass="22585" checksum="EFC2426D2575156A" modified="2022-05-25" version="1">MAAKGTTEMEVGGDGVAVITICNPPVNSLSIDVLLSLKQSYEEALQRKDVKAIVVTGKGGKFSGGFDISSFGDLHSGKIEQPKVGYVSIDILTELLEGATKPSVAAIDGLCLGGGLEVSMACHARISTPTTQLGLAELQLGIIPGFGGTQRLPRLVGLAKSLEMMLLSKPIKGEEAHQLGLVDALVSPNYLVNSARRLALDICELRKPWIKSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B245</accession>
    <name>A0A8I7B245_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123425132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044964741.1">
        <property type="nucleotide sequence ID" value="XM_045108806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B245"/>
    <dbReference type="SMR" id="A0A8I7B245"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0108050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0108050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0108050"/>
    </dbReference>
    <dbReference type="GeneID" id="123425132"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0088660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0088660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0088660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0108050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0108050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0108050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425132"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054292">
        <property type="entry name" value="DUF7028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059153">
        <property type="entry name" value="NSD_PHD-1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF24">
        <property type="entry name" value="OS07G0693650 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22970">
        <property type="entry name" value="DUF7028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23011">
        <property type="entry name" value="PHD-1st_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="334"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0108050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="638" mass="69277" checksum="D27253EE06D2C83E" modified="2022-05-25" version="1">MAIAEYVEFMAGGGRGPGGDELRRRARDSLLSSGWTLYKKAKCDGRQELRYQAPHGASYISLIVACKKFQQFSTAPPRSRTSGKPGMKRKSAAPDCSSRKSKEINAENGGRETGFGGGEDERGPAVRNPFSRECCLAKHRKMRRVSALYADDALEKYAARKRKKASSAPPQLCAASPAASLVRVLRPRTKDGDKAQAAAAAAAAACQPTRSRTIIAVLMDKKIVPPTAKLSYKRTRDEPPVKQGTLTAQGTIRCACGCGRKSFTVAEFAAHALGGECGAERPSASVYLNDGRSLSQCLVQLMRAHGGGGNKTMGSPSPRARLKRKCPPELEGGDFVCSVCADFGDVLLCDCCPSTFHHGCVGLDATPQGEWFCPSCRCAVCGSSEFDLDADGEFTDKTAIYCDQCEREYHAGCIRSRGDRLECCPEGPWLCSHDCSNVFQRLQGLVGRSIPTSVEGLTFIVLRSTKLPEEEAMAAEEHGKLCAAFDVLHECFVTLIEPQTGTDLSHDIVFNRESELRRLNFRGFYVVGLEKGGELITVGTLRVYGKKVAELPLVGTRFAHRRQGMCRLLINQLENLLGELGVERLVLPAVPELLQTWTGSFGFQAMSHSDKMEIAEHTVMCFQGTTMCQKFITAAAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3P7</accession>
    <name>A0A8I7B3P7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3P7"/>
    <dbReference type="SMR" id="A0A8I7B3P7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0157510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0157510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0157510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0190140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190140"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053151">
        <property type="entry name" value="RNase_H-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723">
        <property type="entry name" value="OS05G0353850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723:SF24">
        <property type="entry name" value="RNASE H TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12834" checksum="79C375E022FB1796" modified="2022-05-25" version="1">MILRDEKGGVIFASYRKLFHCNEALEVELQYMMEGLKLSLEHTNSTILLQSDCAMALKTISEASIDSSAYGHLVSEIKSYFSTRVVIPVKIIREQNRVAHCLTNYDRCGDSIAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7G6</accession>
    <name>A0A8I7B7G6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Beta-fructofuranosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123427358</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7G6"/>
    <dbReference type="SMR" id="A0A8I7B7G6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0184450.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0184450.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0184450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0184450.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0184450.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0184450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.560:FF:000002">
        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050551">
        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="8">
        <location>
            <begin position="299"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0184450.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="51945" checksum="9E620DD04EB3CA17" modified="2022-05-25" version="1">MVPDHWYDINGVWSGSATQLPDGRIVMLYTGSTEDAVQVQLLAEPADPSDPLLRRWAKSESNPVLVPPPGIGLTDFRDPTTAWLNPTDRAWRITIGSKNQEHAGLALVYRTEDFLHYDLLPALLHVVQGTGMWECVDFYPVSADLAADVGLDTSTAPGPGVKHVLKASLDDDRHDYYGIGTYDAGTDRWTPDDAAIDVGIGLRYDYGKFYASKSFYDPVGRRRVLWGWIGESDSERADVLKGWASLQSIPRTVLLDTKTGSNLLQWPVVEVENLRMRGKLFDGLDMPPGSVVPLDVGRATQLDVEAVFQVQAGAPAAGSAAAGAEAPYNCSASAGSAGRGLLGPFGLLVLADDGLSEQTAVYFYLVRGADGKLSTHFCQDAFRSSKANDLVKAVYGSSVPVLDGEDLSVRILVDHSIVESFAQGGRTCITSRVYPTKAIYDSARVFLFNNATNLNVTAKSIKIWELNSAYIRPYPYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8F3</accession>
    <name>A0A8I7B8F3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8F3"/>
    <dbReference type="SMR" id="A0A8I7B8F3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0173550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0173550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0173550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0208730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051863">
        <property type="entry name" value="HIPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811:SF74">
        <property type="entry name" value="HMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="141" mass="15473" checksum="6852C5E36A3EB8FF" modified="2022-05-25" version="1">MKKVVLKLDLHDDRQKQKALKAVSGLHGIDLMSVDMKDQKMTVVGSMDPVDVVAKLRKSFPGAHIVSVGPAKEEKKDDKKDGAGGDKKDGGGGDKDKKDASKQVVYAAPYWCPPPPQQHHHPHPYYFVRSAEEDPNSCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9A2</accession>
    <name>A0A8I7B9A2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123396882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="7">subsp. vulgare</strain>
        </source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="8">subsp. vulgare</strain>
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    </dbReference>
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        <location>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5035297011" evidence="5">
        <location>
            <begin position="26"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="61"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="182"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="272" mass="29759" checksum="3C0267493EBE9A25" modified="2022-05-25" version="1">MAGVSANASALVALLLSVLVASVRCAANYGTSAARSYNSGWLPAKATWYGAPTGAGPMDNGGACGFKNVNKYPFSSMTSCGNEPLFDGGAGCGSCYEIRCVAANNPSCSGQPRTVVITDMNYYPVARYHFDLSGTAFGAMAKYGLNDKLRHAGIIDMQFRRVRCNFPGMKVTFHVQRGSNPNYLAVLVEYANVDGTVVRMELMQTRNGRKTGYWEPMRRSWGSIWRMDTSRGLQGPFSMRITSDSGKTLVANNVIPAYWQQDKAYWSNVQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEL1</accession>
    <name>A0A8I7BEL1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123397979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948423.1">
        <property type="nucleotide sequence ID" value="XM_045092488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEL1"/>
    <dbReference type="SMR" id="A0A8I7BEL1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0482600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0482600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0482600"/>
    </dbReference>
    <dbReference type="GeneID" id="123397979"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0400010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0400010.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0400010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0482600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0482600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0482600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397979"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001780">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g30825, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001331">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001509">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46862:SF5">
        <property type="entry name" value="OS02G0170000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46862">
        <property type="entry name" value="OS07G0661900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="542"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="612"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="752"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="787"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="822"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="857"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0482600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="104226" checksum="982D841EA7760A86" modified="2022-05-25" version="1">MGLDLVGFSSRVLPTSAGYAADRRHARGGAAASHGFFAWRSHPRTTLKDSVVCSTEDGGGGGAESTLCVSGPSENASRATGGVAADLGRGIADGITASQTSSMGRKKGGRLWRRPSGGDKPARAPRRAPVREDPQVRRVLVNNPDVNAILSGVSRDSSIAECNSVLIRLEKHSDVKALDFFVWMKANGKLERNADAYRLALQAIAWKEDWRRAELLLHEMVAVSGCRLDAGAFNGLIYVCAKRRLADWGTKWFHMMLERKVQPNVSTVGMLMGLYQKTGNLKEAEFAFVKTRECNVKCVNAYSAMITLYTRSRLFDKSEEVITLMKHDGVVPNMENWLVQLNAYSQQGKMEEAELVLQSMEDGGVSPNVVAYNTVITGYGKVSDMQKAKEVFDRLGSSGLAPDETTYRSMIEGFGRADKHKEAISYYKKLKSSGFQPNASNFYTMINLIARHDDSESAAEILRDMRAAGCQCSSIVTILVRAYRTVGRMHKVLPILQSCFYKKVLYDATSCSILVTTLVQNSLLEEALCVLREKKWQDSDFEDNLYHILICSCKETGSYEDAVRIYNQMPESQTHPNLRICCTMIDVFSAMERFTHAETLYLELKVSACVLDMIAYSVIVRMYNKAGRPEDACSVLEDMEKQKEIVPDKYLFLDMLRTYQKCGMLEKLTDTYYRILKSEVEWDEAMYNCIINCCGPAIPVDELSRIFDEMIQLGHLASTVTLNVLLDIYGKAGLFNRAEKVFSMARKQGLADTISYNTIIAAYAQSGNFRSMNYFVQKMQDAGFPVSLEAYNCMLNAYGKSGQLEKFSAVLQKMRRARCDFDHYTYNIMMNIYGRKGWIEGVAYVLSELKSRGVEPDLYSYNTLIKAYGIAGMPEDAVKLMQEMRIKGINADRVTYTNLIAALQRNENFLEAVKWSLWMKQTGVVGVGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJY0</accession>
    <name>A0A8I7BJY0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJY0"/>
    <dbReference type="SMR" id="A0A8I7BJY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0737350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0737350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0737350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0737350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0737350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0737350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="48"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0737350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25116" checksum="25F6273948D1FB04" modified="2022-05-25" version="1">MPTRFEEVLAHGTTMLDVVYTNESREMPFFLEQLKEGWLDAAMDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSNKHCPELMDFLRSGITFSTVDIRNDKMKMRHNFGIEIPTGCLIDLQTIFRLRHVRTSMAHMAVALIDEEYGDMKTSLPKSQHKLWEKAPLDRINIEYAAKDAYVSHELYRKIRVVNYGHRHLEEGGHFDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BK35</accession>
    <name>A0A8I7BK35_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARPC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BK35"/>
    <dbReference type="SMR" id="A0A8I7BK35"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0745360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0745360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0618130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0618130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0618130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0745360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0745360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0745360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.10">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036753">
        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69060">
        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28519" checksum="52389F16933F77B0" modified="2022-05-25" version="1">MSASATCLLPFVITIPLQLFAIARLIISSMQSLNCCRPTGGISQLPRKLFKASQPSSIDATQEIYVGGVPCLSAVHRTRRSFIAMVYHSSFIDDDGITKACGCPLLPLKTHIKGPAPASDSDKADIIDEAITFFRANVFFKNFHVKSPADKLLIYLTSYINIALKRLETCRTLAVGTKAIINLGLEKVPVPGEPGFPFPGLFTLPQSQEEAELLRNYLKQIREETSGRLLNCAYRANGTPNKWWLAFAKRKFMNVVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-06-12" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>A5CFY2</accession>
    <name>A5CFY2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tua4</name>
        <name evidence="20" type="synonym">LOC123452449</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2007" name="Physiol. Plantarum" volume="131" first="571" last="580">
            <title>Distinct tubulin genes are differentially expressed during barley grain development.</title>
            <authorList>
                <person name="Radchuk V.V."/>
                <person name="Sreenivasulu N."/>
                <person name="Blume Y."/>
                <person name="Weschke W."/>
            </authorList>
            <dbReference type="PubMed" id="18251848"/>
            <dbReference type="DOI" id="10.1111/j.1399-3054.2007.00976.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2007-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Radchuk V."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AM502847">
        <property type="protein sequence ID" value="CAM58977.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A5CFY2"/>
    <dbReference type="SMR" id="A5CFY2"/>
    <dbReference type="PaxDb" id="4513-MLOC_52788.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0426940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0426940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0426940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0513830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513830"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="OMA" id="WRDISAC"/>
    <dbReference type="OrthoDB" id="1853138at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A5CFY2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="17">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="18">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAM58977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="451" mass="49760" checksum="BFDDD43226368541" modified="2007-06-12" version="1">MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQTSGDKTIGGGDDAFNTFFSETGAGKYVPRAVFVDLEPTVIDEVRTSAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTTSLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVSEITNSAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVVEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEDGEEDDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2CPN2</accession>
    <name>F2CPN2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123407512</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK353584">
        <property type="protein sequence ID" value="BAJ84803.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956594.1">
        <property type="nucleotide sequence ID" value="XM_045100659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CPN2"/>
    <dbReference type="SMR" id="F2CPN2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0746810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0746810"/>
    </dbReference>
    <dbReference type="GeneID" id="123407512"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0619460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0619460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0619460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0746810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0746810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0746810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407512"/>
    <dbReference type="OMA" id="NACDFRC"/>
    <dbReference type="OrthoDB" id="1905076at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CPN2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF67">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="87"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ84803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="27243" checksum="6C56530D3EFF64DA" modified="2011-05-31" version="1">MDDGDIDFSNPETYLCEAMGNDPPASCSMGSYFDDILNSDADHLACTHTHTCNPPVHDLAHHTHTCVHVHTKILSASDDAAETSESLPDAKKQRPSGNRAAVRKYREKKKAHTALLEEEVAHLKAVNQQLVKKLQSHSALEAEVARLRCLLVDIRGRIEGEIGTFPYQRSVKSNEFVDQGSFLGGAQVMNSCDFRCNDQLYCNPGMQQARTMDDDGVMSAGQVLGQGAGDTMGCVKPGSLNPPGCRGGQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQ09</accession>
    <name>F2CQ09_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123433209</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK353711">
        <property type="protein sequence ID" value="BAJ84930.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971374.1">
        <property type="nucleotide sequence ID" value="XM_045115439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQ09"/>
    <dbReference type="SMR" id="F2CQ09"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0047710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0047710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0047710"/>
    </dbReference>
    <dbReference type="GeneID" id="123433209"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0037230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0037230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0037230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0047710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0047710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0047710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123433209"/>
    <dbReference type="OrthoDB" id="2020333at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36348">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36348:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ84930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0047710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="329" mass="37351" checksum="4EEE44F2FD1F8473" modified="2011-05-31" version="1">MATATASTSLRPPPSPSPSLPRLAGSLRQWCWGTPTHSSRRRAFHAHRRKRNTLVCAAVKGPEESLKKTIEVDRLIDMLRDANPGELDLIVVENILAFSEAFWVRLAARIDLCKSDDDKKDYEELAENVMNIVDRVVHKTDEKIEQSTDVLKAIISPVMNEGEDAMWPPRNPEALKLMEKEISNREKEGQLDESFLSEVNAQLRQAKEDVDKPGLQVMLQKVLQLYASNFLQKRSYAYKGGEVVVPEKFLESIIEAPENDWNRLLLDGLTVGKGDVSPEEFYAVTKKRIERILIRTEGGSYQQRVLVEYIKEIQARAEEIVNRLQGPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2CT29</accession>
    <name>F2CT29_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123410592</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ86527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0265300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="240" mass="26385" checksum="9958B6104938B93F" modified="2011-05-31" version="1">MSPPTKKERAAEARASSSKPIVAGPPQVQRRRVSGGGWTSRRISFYASRVYFVLIILQIPLFRVPCRAGTCTTPIQITSSQLVSNEIFPPSVVKAMLYPGAIVRNLTTSMSMTFPRWSDLFDIYNLTEAKNASAVIDLQRLEMLAGSYFCVAGALVGIINPGRMILFGTLLVIWGLVKDALFRKPVISDPTEPVYVYPTILIALICSFMSITYNVKKKAMSSQSVSISKPLQSSAKSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXQ9</accession>
    <name>F2CXQ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123425931</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK356412">
        <property type="protein sequence ID" value="BAJ87630.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965631.1">
        <property type="nucleotide sequence ID" value="XM_045109696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142190"/>
    </dbReference>
    <dbReference type="GeneID" id="123425931"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0117130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0117130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0117130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0142190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0142190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0142190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425931"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF8">
        <property type="entry name" value="POTASSIUM TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="558"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="12">
        <location>
            <begin position="109"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="13">
        <location>
            <begin position="610"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="710"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="715"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ87630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0142190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="853" mass="94298" checksum="A0358D6D6ABF6C8F" modified="2011-05-31" version="1">METGSSGGRRLPKTESAEMRWVVSGGASEDDEIDSSDDGDGGTDTPTAALGSRGGGGGYSDAEEDEEDALLRQRLVRTGPRADSFDVEALDVPGLYRHQEFTVGRSIVLALQTLGVVFGDVGTSPLYTFDIMFNKYPNTSKEDVLGALSLVIYTLILIPLLKYTLIVLWGNDDGEGGIFALYSLICRNAKASLLPNQLPSDTRISSFQLKVPSVELERSLRIKERLETSSMLKKLLLMLVLFGTSMVIADGVVTPAMSVMSAVNGLKVGISSVNEGEVVMISVAFLIVLFSLQRFGTSKVGLAVGPALFIWFCCLSGIGIYNIIKYGTEVLRAFNPIYIYYYFEKNPTQAWMSLGGCLLCATGSEAMFADLCYFSVRSVQLTFVCLVLPCLLLGYLGQAAFLMENLTENEQVFFLSIPSQVFWPVVFIATLAALIASRTMTTAIFSIIKQATALGCFPRLKIIHTSRKFMGQIYIPVMNWFLLVSCLAFVTTFGSINEIGNAYGIAELGVMMMTTILVTIIMLLIWQVNIIVVLCFLTLFLGLELFFFSSVLGSVADGSWVLLVFAAVLYLVMYIWNYGTKLKYETEVKQKLSMDLMMDLGCNLGTVRAPGIGLLYNELVRGVPAIFGHFLTTMPAIHSMIIFVCIKWVPVPVVPQNERFLFRRVCPKNYHMFRCIARYGYKDVRKENPQTFEQLLIESLEKFIRREAQERSLESDENGNTDSEEEVGSTSSRVLVGPNGSIYSLGVPLLAESAGVSNPNLGSSTSFDGSLDGTMDGRRSLDNELSFIHKAKECGVVYLLGHGDIRARKESFFAKKLVINYFYAFLRKNCRRGIATLSIPHTRLMQVAMQYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2D320</accession>
    <name>F2D320_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123396032</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK358277">
        <property type="protein sequence ID" value="BAJ89491.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946996.1">
        <property type="nucleotide sequence ID" value="XM_045091061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D320"/>
    <dbReference type="SMR" id="F2D320"/>
    <dbReference type="STRING" id="112509.F2D320"/>
    <dbReference type="PaxDb" id="4513-MLOC_58790.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0429510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0429510"/>
    </dbReference>
    <dbReference type="GeneID" id="123396032"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0356880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0356880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0356880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0429510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0429510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0429510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396032"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_6_0_1"/>
    <dbReference type="OMA" id="EMEWALI"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000028">
        <property type="entry name" value="Glutaredoxin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ89491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429510.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="11119" checksum="C213C865FD841E2A" modified="2011-05-31" version="1">MEQVTKLAGQRAVVIFSMSSCCMCHTVTRLFRDLGANPAEVDLDEDPRGKEMEKALARLLGRNPAVPAVFIGGRLVGSTDKVMSLHLSGKLVPLLRNAGAVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2DET8</accession>
    <name>F2DET8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123409318</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362405">
        <property type="protein sequence ID" value="BAJ93609.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958191.1">
        <property type="nucleotide sequence ID" value="XM_045102256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DET8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677120"/>
    </dbReference>
    <dbReference type="GeneID" id="123409318"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0561930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0561930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0561930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0677120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409318"/>
    <dbReference type="OrthoDB" id="77038at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056866">
        <property type="entry name" value="Znf_WRKY19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31827">
        <property type="entry name" value="EMB|CAB89363.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31827:SF43">
        <property type="entry name" value="OS06G0472700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24906">
        <property type="entry name" value="Zf_WRKY19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
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        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ93609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="447" mass="46659" checksum="54B83CE9F56D5BB7" modified="2011-05-31" version="1">MSGPPVFSVLQGSNTTHRGQQISMIGNGHSFQQGVYVMDPSSFNQGIYAEKQSSFAERTNMIQLNNCMADNSSPAGNRCQRLGCNEVVEGQTAFCKSHRQCQMIGCPQVPPNGVAFCMAHGGGHLCNDPGSSVVPFTESEGSIKYEGDSRFRVMENAMVSTGINNPDGEVVMCKFQGCSKRSQGNTMYCKVHSGGSKACMVQGCTKGAHGGTPMCIAHGGGKRCSVAGCRNAACGSSQGRTDCCVRHGGGKRCKHDGCGKGAQGNTDFCIGHGGGRRCKFEGCGKSAQGRSDNCIKHGGGRRCKFEGCSASAKWGVDFCSAHRKGMLGGNDTADGAPKPKRRAKKSAGTKKAEKAKRAKKTVVGPAGLSGEDATMPAVLSADMPEMGAIHVATHVSDRPKSPETAIALQQPPVQLQQPPPLQSLEPSGLTASTEEGLPVAGSVFFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFH0</accession>
    <name>F2DFH0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362637">
        <property type="protein sequence ID" value="BAJ93841.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DFH0"/>
    <dbReference type="SMR" id="F2DFH0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0141340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0141340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0141340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0171170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905761">
        <property type="term" value="F:SCF ubiquitin ligase complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000037">
        <property type="entry name" value="F-box protein SKIP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF28">
        <property type="entry name" value="F-BOX PROTEIN SKIP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="28"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ93841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="319" mass="35393" checksum="688B5904755017EF" modified="2011-05-31" version="1">MAAPAPSPSPSPSRHHRPDEPPSEEEAARDWAGLPWDALLAVLHRLDHVDVLMGAGQVCSPWRCAARDEPELWRRVEVRSHADRRPFHTAEPSRARRHSCAVLCGLARAAVRRAAGQCEAFCGEGAADDSVLSLLADEAPSLKSLRIISGDRIVDGRLRLTVTSFTLLEELELSLCTDVYPGTCEAVGSACPRLRRFRLSKDGFCKWYTKNIDQEAMAIATMRGLRSLQLFANPLSNDGLAAILAGCTRLESLDIRHCFNVGMGAAAIRARCPGIHTLRLPRDSTIDYDLKFSPQDMTPWNTQDENYCDWHDLIYCSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2DHM7</accession>
    <name>F2DHM7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395828</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363394">
        <property type="protein sequence ID" value="BAJ94598.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946798.1">
        <property type="nucleotide sequence ID" value="XM_045090863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DHM7"/>
    <dbReference type="STRING" id="112509.F2DHM7"/>
    <dbReference type="PaxDb" id="4513-MLOC_40112.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0459480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0459480"/>
    </dbReference>
    <dbReference type="GeneID" id="123395828"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0380330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0380330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0380330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0459480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0459480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0459480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395828"/>
    <dbReference type="eggNOG" id="ENOG502QQGE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="GPLKGCM"/>
    <dbReference type="OrthoDB" id="2018987at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021864">
        <property type="entry name" value="DUF3475"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007700">
        <property type="entry name" value="DUF668"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31371">
        <property type="entry name" value="BNAC09G50660D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31371:SF20">
        <property type="entry name" value="OS12G0146500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11961">
        <property type="entry name" value="DUF3475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05003">
        <property type="entry name" value="DUF668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3475" evidence="2">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="DUF668" evidence="1">
        <location>
            <begin position="377"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ94598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459480.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="545" mass="59658" checksum="30662040739E646E" modified="2011-05-31" version="1">MRKLSTGRGGGERVAMLSFEVAALMSRAASLWRALEEDQLARLRGEGVRLEGVRRLVADEDSALLALAVGEMAGACGDISCAVARLSGRCADPLLRRFDELYAGLIAGGAGADSHRLRYAAAKKMDRKARKMQRLVAATGLLCQEIDVLAELEQGARLRRAQFAPGEAARRVARQRQEVDRLRAASLWNRSLDYAVRLLARSLFTIVARIIDVFDLQPKKIAMNDYSMVSPAGARLSFSWSNSFVGSTNSLVYPTDFPVDARKRSTGAANSGKAPNGDVRRFLLSRSKSLRQLKWPVRGKHLIGCVVSGSKSPTREGWVHGGHDLPLSFSYVSSNNEDSSVNYQSNESDRHSASTKLSTSVFECSSHDVLANGPETTLGAAALASHYANLVVFAEKLAISPRHICPDERDALYGMLTDSIRASLRARLRPPSSAARKKDTACDRVLAAEWADTVQGILGWLAPVAHNTVRWRSERSFEQRNVGSGTSVLLLQTLHFADRDKTEDAITELLVGLNYLWRYGTQLSAKPKLESVGGDVYHDRADYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTV8</accession>
    <name>F2DTV8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123399206</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AK367326">
        <property type="protein sequence ID" value="BAJ98529.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949571.1">
        <property type="nucleotide sequence ID" value="XM_045093636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTV8"/>
    <dbReference type="SMR" id="F2DTV8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489580"/>
    </dbReference>
    <dbReference type="GeneID" id="123399206"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0405640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0405640.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0405640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0489580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399206"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000006">
        <property type="entry name" value="Nuclear transcription factor Y subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF150">
        <property type="entry name" value="OS09G0480700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="82"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ98529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="203" mass="21782" checksum="B1F7E3EFEB33C6E1" modified="2011-05-31" version="1">MDPTKSSTPPPPPVLGAPVGYPPGAYPPPPGAAPAPYPPQLYAPPGAAAAQQAAAQQQQQLQVFWAEQYREIEATTDFKNHNLPLARIKKIMKADEDVRMIAAEAPVVFARACEMFILELTHRGWAHAEENKRRTLQKSDIAAAIARTEVFDFLVDIVPRDDAKDVEAAAAAAMATAAAGIPRPAAGVPATDPSMAYYYVPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DUH8</accession>
    <name>F2DUH8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444941</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK367546">
        <property type="protein sequence ID" value="BAJ98749.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977767.1">
        <property type="nucleotide sequence ID" value="XM_045121832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DUH8"/>
    <dbReference type="FunCoup" id="F2DUH8">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2DUH8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302980"/>
    </dbReference>
    <dbReference type="GeneID" id="123444941"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0252800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0252800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0252800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444941"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DUH8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="244"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="460"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="512"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ98749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="532" mass="59944" checksum="61AE2DF408E3B645" modified="2011-05-31" version="1">MMGGVLLDHFRSSAWFYLTPVLAACAPIGVLRTYFNQHLRRPVRRLLPFLDPFVTIDIAAKPEDYSYSYQGKVKSSDAYAEVLAYLSAVCSREARELRAEGAVEGHGFVLSLREGQEVADEFKGVTMWWSAVAEEKATWRASGRCCRLTFHERHRRLVVDEYLPYVRRAGQEVTFGNRPRRLYSNKKELNYHSRRDEVWSYIDFDHPTTFDTLAMDPAKKQMIMDDLEDFANSKDYYRQIGKAWKRGYLLHGPPGTGKSTMIAAMANHLNYDIYDIELTTLETNSDLRKLFIETTGKSIIVIEDIDCSLDLTGSRATKLPPPPAHDDAADGNDKSRKRRNILTLSGLLNFIDGLWSAHSGERIIVFTTNHLDKLDPALIRRGRMDMHIEMSYCGFEAFRTLAGNYLGVDAHPLFGAVGELLRAVEMTPADVAECLMPSKRSARDADACLARLIDQLKEKAAEKDKESKAAEEGDERDAAKEDDKTETEKVASMSKKEKSEEKKQSCRVMTNGARSGASAVMSVSSSTDHYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWG4</accession>
    <name>F2DWG4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123443857</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368232">
        <property type="protein sequence ID" value="BAJ99435.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976330.1">
        <property type="nucleotide sequence ID" value="XM_045120395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWG4"/>
    <dbReference type="SMR" id="F2DWG4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270870"/>
    </dbReference>
    <dbReference type="GeneID" id="123443857"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0224500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0224500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0224500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0270870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443857"/>
    <dbReference type="HOGENOM" id="CLU_008664_0_0_1"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF85">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4 HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="236"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ99435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="912" mass="101224" checksum="AAE93FB06A1F2493" modified="2011-05-31" version="1">MRVVPSAFLRRAASMSSHYNRRGSSRPHRGYSSRPPPPSAYADAELVSGDSHLSTVPAANDSLRRGGGPRASPPQYRHGPQLQPPPYGYGYGQPQPHPQPQPQVQPYGFVPYNYSHPPQGPFPGPQYGYGTPNQYGHWHPQPYRVVPPNGGFLPRNAGFRPAAPQLQSRLAQYKREWRSVQKLPPRHAERFKVLSYNILADYLAQEHQDLYRDIPSFIMDWNWRKNRIGLEISCWRPDIICFQEVDKFTDLEQEMSTRGYTGIWKMRTGNAVDGCAIFWRTARFQLCYKEDIEFNKLGLRDNVAQLCVLESVFQRNVQTGSTHLSTSSIHPQQAKQVVICNIHVLYNPKRGDIKLGQIRTLLDRAYATSKRWNDAPVILCGDFNATPKSPLYNYILEQKLNLFGLARNAISGQQTSSHGLYTGSNTSRYTFRPPLHMTNGREGRMITLDVHKPQSEAKSLVRDSCLAGREPVLTAIASTSCLNSESSKYFGNNRPCSGPSNLDEQGLSSCLSGLAKDACNSDGAAECEEGAAVDSSSEECFEGIKVESREPDIGGVQCSQTAVYDEAIQSDSSEAISRHLLSSELSGRIDSVGELRCVSSKDPNSLGDLSGNVICEDVTGGFEGNSAQSDTLLNVSKENPGEKEKCNESMSGQNNCTTPKSESSHFSDSLKSADARCKMSNMRVDEEINTGSTHLTSPVEQLTHQTNSATSDSCGNQCTPEVTNKHLDSYSCPEEFGNHACSVEDDVTANENLCTNVLSDPTSFKEFAGDNECLHLDKNQLPTISNGSRHAHKVVPYGGYYNDPYRWTVDEMKAATGKEECTYVEHNLKVRSVYTDVEDFHGTKDANKEPLVTSYNRKFMGTVDYIWASEDLQTVSVLDTFPEGILKETIGFPTKKWGSDHIALVCELAFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECC1</accession>
    <name>F2ECC1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123439951</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Seed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK373796">
        <property type="protein sequence ID" value="BAK04993.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972499.1">
        <property type="nucleotide sequence ID" value="XM_045116564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECC1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270610"/>
    </dbReference>
    <dbReference type="GeneID" id="123439951"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0224260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0224260.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0224260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0270610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0270610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439951"/>
    <dbReference type="OrthoDB" id="1898295at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057710">
        <property type="entry name" value="DUF7950"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33595:SF7">
        <property type="entry name" value="OS12G0242500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33595">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25821">
        <property type="entry name" value="DUF7950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7950" evidence="2">
        <location>
            <begin position="176"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK04993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="312" mass="32249" checksum="A6BDA23429AFE475" modified="2011-05-31" version="1">MMERKGGCCLAPRYAAGAQAGQAWHLGKVVLKFRPIAPKPAAMAPAPVTPAPVTPVPAGKEKRKRKAVAGPGGTGRRGRKPKKSAAVAVAAAAAASTTQKLDYVQDKPLSSPSSSSSGTTSVSSPPPPPLPATLALMPVLPAQEGFGGAAPVANLAPAHVARLVLPPQALRQPVPWVTVEDVTGIWRHGEEPYAAVCGGEEAPTFVSDQCGRVTWTNVAFNRTVSGREDDDAAMAPSAAASEVRVVLAAKDGTPVPAWGSCAGFTCRVRVPYACPRRGSLVAPCDVWRLDAGGYLWRLDLQATLSLSLGGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F2EE44</accession>
    <name>F2EE44_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="AK374420">
        <property type="protein sequence ID" value="BAK05616.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EE44"/>
    <dbReference type="SMR" id="F2EE44"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0246180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0246180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0246180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0204380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0204380.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0204380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0246180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0246180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0246180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EE44">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF395">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="34"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK05616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0246180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="41195" checksum="14476DCC160DAEFA" modified="2011-05-31" version="1">MATTKRSAAAVDGHADRPGAACVKKRRVSTTGDYDHESCLGEGMYGVVDKRRHRATGEAVAIKSFRNPYNKDEPVDAEEVLREARFLEACGGHPHIVGFRGVVQDYVTNELCLVMEYVQGKSLYCHLRERRGGLPEATVRDFMWQLLTAATKMHRCQVVHRDIKPANIIVGEGGVLKLCDFGVALSMSEAPPYRQAGTGMYRAPEMLLGKRDYDALVDMWSLGCVMAETITGQGLFDEDNSICLIRRIFEVVGMQDDTTWPWFMSLPLAEEVPQVQTNTLRELFPEETLSKDGFEVLSGLLACNPDKRLTAAAALKLPWFTTIAANARPLASRPAAATATVSVKVEEVEIVPSTLPRKKVTAKKYVSLFVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2EI18</accession>
    <name>F2EI18_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123448151</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Flower</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375795">
        <property type="protein sequence ID" value="BAK06990.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980876.1">
        <property type="nucleotide sequence ID" value="XM_045124941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EI18"/>
    <dbReference type="SMR" id="F2EI18"/>
    <dbReference type="STRING" id="112509.F2EI18"/>
    <dbReference type="PaxDb" id="4513-MLOC_60544.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0355140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0355140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0355140"/>
    </dbReference>
    <dbReference type="GeneID" id="123448151"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0296280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0296280.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0296280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0355140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0355140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0355140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448151"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_4_1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF87">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="93"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK06990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0355140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="43194" checksum="EB1FDA2CE138B885" modified="2011-05-31" version="1">MLSWLRCFPHDGTAMDEERKPRPGRSATFRKKHCPDAAAARKRFIRSGTSLTASSSARASFGRHSVDVANYNHSIVSARSFTFRELGAATDSFSQANLIGEGGFGRVYRGLIGSSAVAVKQLDRTGFQGDHEFLVEVLVLSSLLTHPNLVGLLGYCADGNQRLLVYQLMPLGSLENHLFLPQPQTQAPADGDGKQVLPWRTRMRIAHDAAQGLEFLHETANPPVIYRDLKSSNILLDEGYNAKLSDFGLAKLATPITRNGKGGEAEEDKDGSRVMGTYGYCAPEYVRMGHLTVKSDVYSFGVVLLELITGRRAIDDSRPEGEHNLVAWAAPMFGEQRRLQELVDPLLGQGPSGRELKQAVAVAAMCLQEEDTVRPIMSDVVMALSFLAADDDMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2EI88</accession>
    <name>F2EI88_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123408683</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK375865">
        <property type="protein sequence ID" value="BAK07060.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957678.1">
        <property type="nucleotide sequence ID" value="XM_045101743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EI88"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708840"/>
    </dbReference>
    <dbReference type="GeneID" id="123408683"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587950.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408683"/>
    <dbReference type="HOGENOM" id="CLU_139400_0_0_1"/>
    <dbReference type="OMA" id="HRDANAW"/>
    <dbReference type="OrthoDB" id="690994at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EI88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35704">
        <property type="entry name" value="OS02G0254600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35704:SF6">
        <property type="entry name" value="OS06G0529900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK07060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="131" mass="14934" checksum="BF394CC21ABEF74E" modified="2011-05-31" version="1">MGNCLNKASTQQQQREMRRRRHGHGERVAPEEREEEEELRSIGQVLLQEEEEEEEEAPAAASAAGMKVKVVLTRAELEWLMAQLKSGEQRLEDVLRQMGNARDVDDKPPRADGWRPRLECILECPEAADAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F2EIZ1</accession>
    <name>F2EIZ1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123428284</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376118">
        <property type="protein sequence ID" value="BAK07313.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968412.1">
        <property type="nucleotide sequence ID" value="XM_045112477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EIZ1"/>
    <dbReference type="SMR" id="F2EIZ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208700"/>
    </dbReference>
    <dbReference type="GeneID" id="123428284"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0173530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0173530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0173530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0208700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428284"/>
    <dbReference type="OrthoDB" id="686027at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051863">
        <property type="entry name" value="HIPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811:SF21">
        <property type="entry name" value="HMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK07313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="162" mass="17293" checksum="F14B4CC8D98A73D7" modified="2011-05-31" version="1">MKKVVLKLDVHDDRHKAKALKAVSGLHGIDQLGVDMKDQKMTIVGTVDPVAVVGKLRKLFPGVQIVSVGPAKEEKKDDKKDGGGDKKPGGDKKDGDKKDGGGGDKKDGDKKAGGDKKPQQDAKPAMPVYPPYGYAHYGYPPPPPPRYVVHSAEDDPNSCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>M0WAY2</accession>
    <name>M0WAY2_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123440661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973157.1">
        <property type="nucleotide sequence ID" value="XM_045117222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WAY2"/>
    <dbReference type="FunCoup" id="M0WAY2">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_4701.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308770"/>
    </dbReference>
    <dbReference type="GeneID" id="123440661"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0257750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0257750.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0257750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0308770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440661"/>
    <dbReference type="eggNOG" id="ENOG502R3IY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WAY2"/>
    <dbReference type="OMA" id="HGNDYLM"/>
    <dbReference type="OrthoDB" id="661594at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="91" mass="10460" checksum="4CF169907E967767" modified="2013-04-03" version="1">MAHFQEVDYCSEEVRAVGNPARRGGGVQEHIVKETFVQEFDTAGRHHGHHGHHGRGSGHFEVRESRLEEDINTRTGEFHERKENFVIRADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>M0WD89</accession>
    <name>M0WD89_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123402152</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044951967.1">
        <property type="nucleotide sequence ID" value="XM_045096032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WD89"/>
    <dbReference type="SMR" id="M0WD89"/>
    <dbReference type="PaxDb" id="4513-MLOC_4942.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609390"/>
    </dbReference>
    <dbReference type="GeneID" id="123402152"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0505250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0505250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0505250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0609390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402152"/>
    <dbReference type="eggNOG" id="KOG1448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10263753at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WD89">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000032">
        <property type="entry name" value="ribose-phosphate pyrophosphokinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000034">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF34">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="313"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="417" mass="45483" checksum="BAFD29860FB7D1A0" modified="2013-04-03" version="1">MLPSATAANTYYYYYWPGAAAAGGLGFSRAAIIGRAKGESNRYSRSRTTCLCLAARVSIPPTPSPSEPLPTRRSPVPASAAAAACCRDEMEAAAAVAVPRHKVKKQINLFYCAECEELALKVAAASDAIQLQSINWRNFPDGFPNLFINNAHDIRGQHVAFLASFSSPAIIFEQISVIFALPKLFIASFTLVLPFFPTGTFERVEEEGDVATAFTLARILSMIPKSRGGPTSVVIYDIHALQERFYFGDDVLPCFETGIPLLLQRLSQLPDADNVTIAFPDDGAWKRFHKSLANFPVVVCAKVREGDKRIVRIKEGNPEGRHVVIVDDLVQSGGTLRECQKVLAAHGATKVSAYVTHAVFPNQSYERFMTANSAEPGDQFAYFWITDSCPQTVKAISQQPPFEVLSLASSIADALQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y0X7</accession>
    <name>M0Y0X7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123399922</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transcription factor probably involved in vascular development and shoot tissue organization. Binds to the DNA sequence 5'-CCGAGTGTGCCCCTGG-3' present in the promoter region Box II of the phloem-specific rice tungro bacilliform virus (RTBV) promoter. May regulate tissue-specific expression of the RTBV promoter and virus replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950240.1">
        <property type="nucleotide sequence ID" value="XM_045094305.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950241.1">
        <property type="nucleotide sequence ID" value="XM_045094306.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950242.1">
        <property type="nucleotide sequence ID" value="XM_045094307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y0X7"/>
    <dbReference type="SMR" id="M0Y0X7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496250"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496250.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496250.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496250"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496250.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496250.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496250"/>
    </dbReference>
    <dbReference type="GeneID" id="123399922"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0411590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0411590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0411590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0496250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0496250.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496250.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0496250.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496250.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399922"/>
    <dbReference type="eggNOG" id="ENOG502QTK9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="TGQAMSN"/>
    <dbReference type="OrthoDB" id="1435597at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y0X7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000009">
        <property type="entry name" value="probable transcription factor PosF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690">
        <property type="entry name" value="TRANSCRIPTION FACTOR POSF21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690:SF124">
        <property type="entry name" value="TRANSCRIPTION FACTOR RF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="174"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="192"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="339"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="375" mass="40855" checksum="8A59601D2AF9A19B" modified="2013-04-03" version="1">MNKDRSRISADGGGDGLPPQSSRRSATPPPEYDISRMPDFPTRNPGHRRAHSEILSLPDDLDLCAPGGGDGPSLSDENDEELFSMFLDVDKLNSSCGPSSDQAESSSAAAAGGGEAARAGHVMRPKHQHSQSMDESMSIKAEDLVGAPGMDGMSTVEAKKAVSAAKLAELALVDPKRAKRIWANRQSAARSKERKMRYIGELERKVQTLQTEATTLSAQLSLLQRDTSGLTSENGELKLRLQNMEQQVHLQDALNDTLKTEVQRLKVATGQMGNGGGMLMNYGGMSQAPHQFGGNQQMFHNNQSMQSLLATHQLQQLQLHQQPQQQQQPMRPQHHHHQQQPLHPLQAQQFQQAARDLKMKGPMWGDGKSSGGGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-07-05" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q4VM11</accession>
    <name>Q4VM11_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Beta-amylase</fullName>
            <ecNumber evidence="4 12">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bmy2</name>
        <name evidence="17" type="synonym">BAM2</name>
        <name evidence="14" type="synonym">Bmy2</name>
        <name evidence="18" type="synonym">LOC123425805</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Allelic variation in the beta-amylase 2 (bmy2) loci and functional consequences of this variation.</title>
            <authorList>
                <person name="Clark S.E."/>
                <person name="Hayes P.M."/>
                <person name="Henson C.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2006-08" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis of gene expression in barley Bmy1 and Bmy2.</title>
            <authorList>
                <person name="Vinje M.A."/>
                <person name="Henson C.A."/>
                <person name="Duke S.H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="journal article" date="2009" name="Plant Physiol." volume="150" first="190" last="204">
            <title>SPATIO-TEMPORAL PROFILING OF STARCH BIOSYNTHESIS AND DEGRADATION IN THE DEVELOPING BARLEY GRAIN.</title>
            <authorList>
                <person name="Radchuk V.V."/>
                <person name="Borisjuk L."/>
                <person name="Sreenivasulu N."/>
                <person name="Merx K."/>
                <person name="Mock H.P."/>
                <person name="Rolletschek H."/>
                <person name="Wobus U."/>
                <person name="Weschke W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2009-04" db="EMBL/GenBank/DDBJ databases">
            <title>Differential expression of two barley beta-amylase genes (Bmy1 and Bmy2) in developing grains and their association with beta-amylase activity.</title>
            <authorList>
                <person name="Vinje M.A."/>
                <person name="Willis D.K."/>
                <person name="Duke S.H."/>
                <person name="Henson C.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="5">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
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                <person name="Muehlbauer G.J."/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
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        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
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        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="212" mass="23283" checksum="8E625FD7D7099AEB" modified="2017-11-22" version="1">MGTDFSGTLPAARLGPPRARRHGRPRPIIHAARSVRAHCAPDWPIYFPVRSDRSFPFPFLPSAGARDGIMAAACSLSLLPLAPPATPRPALFSRCIAAPRRGRNAGGGTGRLRLARRRLPPPAAAIEEVEQTEAMMRVAADDDSITATVVSVLLTVSFVGLSLLTIGVIYLAVQDFLQKRESEKFEREEAEKQKEEARQKRAKARGKKRNRR</sequence>
</entry>
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    <accession>A0A287QLF7</accession>
    <name>A0A287QLF7_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC123395886</name>
    </gene>
    <organism evidence="2 3">
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Triticodae</taxon>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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        </submittedName>
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            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0041520.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="234" mass="26515" checksum="23AF90EAEB554F24" modified="2022-05-25" version="1">MLVRMMSVSGYRWYPEYTVEEHYRDFNQSQYLCTVRVFPDYLGAEEPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLHNAEFCYVPASQPGEEGYLSGVYFDFSMEDPLLQSTAEMLENRDRDARALRMELYATRARLWMALTQLAPVVQIGYGDMEMLNPVRTHLPAHVDWPAIGGFTPLRGPLLPPVRGPRPHPCPYGSQGSQAGVFPDPQVELPSHGGNLYEMFYADA</sequence>
</entry>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFJ4"/>
    <dbReference type="SMR" id="A0A8I6WFJ4"/>
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0022540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0022540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053151">
        <property type="entry name" value="RNase_H-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
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            <end position="148"/>
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        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="183" mass="20185" checksum="E0DF26EBFB06BAC8" modified="2022-05-25" version="1">MPMVREANVEILSPSTPLPWPRPPTDCAALSVDGAFMQEDGSAAAGMILRRYDGSVIFAAYRCIFNCNDALKAVLHAIMQGMALALQHCSLPIVVQSNSSVALAAMTGESLSRSAYEHLVAEIRHHMEAREFVPSKIKCEQNMVAHRLTLYSRTEFTTIVWLGRSPSCIEELVPLDCNPIHME</sequence>
</entry>
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            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001990.1">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549:SF226">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <location>
            <begin position="101"/>
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        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001990.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="314" mass="35690" checksum="EF4D5BFEA5D11358" modified="2022-05-25" version="1">MDPALSTYFYANFERISTDIMMLENQIPWIMVKIIFSFMRPVLSSPWSCPWEKFVVAMRRGLKNQMVGDDVQDLDIDPMYEPPHLLGLVRFYIVGKYKDDKSDELPPEEHPPEEHPPEEKPMSLSISVAELEDIGIRLVPKTGTEAVLADMRLHSSKWGCFSDLFVPPLCLTEANATSLVNMAALELCIAPDFNDVGAENSAVCSYLHLFAMLLDQERHVQDLRKNKVIEGGGLTSKETLQFFTSIGKNMRHGTSYLHIIIEIDEFKRKRLFLLKAYLFIMKHWTKIIAVTTVIGAVAGILSSLQNLKTKQPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeinae</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="306" mass="32318" checksum="08568BA3E4451345" modified="2022-05-25" version="1">MAGRDLPSLAFAIARLLQKESSRFPRTHRHRVAHRHQGLGARSGPRRRFFARNHCQNRRRGYARRGNQPLRGHAPTVHQAGPSAPATASLHHTGPSAPAVAGDYIVPATPPSAFNIPPMDCLLAASSAPFLGLEEMFPCELAPPPLPPYYMQDEFGLCPARTGAPPWLPSSMPSDKLSEHLFPPGYGPVSDLPSLKPAAAETGGYPAVPYLNGAIKMEEEGEGQGSSSMPPPPSLATPPPPPAPPLPPTPPPEVRHIRRQIIADMAQNRAAVRGTWSSASLGLTGAPGATSNGGGRAAARGTPRLL</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
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</entry>
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            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
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    <organism evidence="13 14">
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            <taxon>Hordeinae</taxon>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044971882.1">
        <property type="nucleotide sequence ID" value="XM_045115947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMU8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223150"/>
    </dbReference>
    <dbReference type="GeneID" id="123439208"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0185460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0185460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0185460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0223150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439208"/>
    <dbReference type="OrthoDB" id="4788989at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044286">
        <property type="entry name" value="SINL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632:SF16">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="80"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="266" mass="29767" checksum="68F5FA9DF960F89E" modified="2022-05-25" version="1">MEAERRNNEKSAASEGAIPLEVLDCAFCYHPLRPPVFQCRVGHVICSSCHHKLRKKDKCHVCSITGGYNRCIAFDHILESIKTSCSNSIYGCTVNTQYYKQEDHEKACPHAPCFCPEPGCGFVGSTTQLQCHLTHDHMLPATAFAYGYCFTLEMQEGMRVLHRKEEGGPLFLAKFTPVPPFGNAVSILCIDPHAMVGKHKFECHVDIYSRTMGWNQHSNFQIISTDLSNGFPGEEASYTFIVPNISSNPHTTTTRLLIRPPNITRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WP55</accession>
    <name>A0A8I6WP55_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding Rossmann-fold superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123424901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044964528.1">
        <property type="nucleotide sequence ID" value="XM_045108593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WP55"/>
    <dbReference type="SMR" id="A0A8I6WP55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0116840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0116840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0116840"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0116840.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0116840.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0116840"/>
    </dbReference>
    <dbReference type="GeneID" id="123424901"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0096030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0096030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0096030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0116840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0116840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0116840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0116840.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0116840.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0116840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424901"/>
    <dbReference type="OrthoDB" id="426386at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000344">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000377">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="171"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="1">
        <location>
            <begin position="310"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="483"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0116840.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="69241" checksum="9A8B6E4A64EE17E7" modified="2022-05-25" version="1">MILCGRGGLSRSNPCLQYHRNRHRRRRACDKAPAARLHSLAAPSVQELKMNCPSSSSSCCGVSPPVLASPRGEFAAGCSTRTTQKAHFLGSRQSSQLIYSPTSSRLSRRAVIALAGKQSWDIGRFAKTLFFFNGPPNPLKIVESIMSSITASAPTEAPKKAETSDVVLVTGATGGVGRRVVDVLRKKGVPVRVLVRNAEKARTMLGPDVDLIIGDVTKGDTLDPKYFKGIKKVINAVSVIVGPKEGDTPDRQKYSQGIKFFEPEIKGPSPEMVEYLGMQNLINAVKESVGLSEGKLLFGFKGNLCGKFVWGALDDVVMGGVSESAFQIQPTGSETGEATGLFKGIVSTSNNGGFTSIRTKNFTVPEDLSAYDGVELRVKGDGRRYKLIIRTSYEWDTIGYTASFDTMKGEWQSIRIPFSSLIPVFRARTATDAPPFDASNITALQLMFSKFEYDGKLNPTFAEGQFELPFSSIRAYISEPITPRFIHVSSAGVTRPERPGLDLSKQPPAVRMNKELGSILTYKLKGEDLIRESGVPYSIVRPCALTEEPAGADLIFEQGDNITGKISREEVARICVAALASPSAVGKTFEVKSTVPFSEPFVIDPSNPPPEKDYEVYFKELKDGITGKEALEGTPALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQQ8</accession>
    <name>A0A8I6WQQ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQQ8"/>
    <dbReference type="SMR" id="A0A8I6WQQ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0024150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0024150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0024150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0024150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0024150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0024150"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0024150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15741" checksum="4667E863802DB847" modified="2022-05-25" version="1">MVLVSAFVGSRPVYDSDVEVCEPEIPTHIKILVEKLNLVEKENKYLKKKLKIIEDNNMELELHVADVIDDRKIKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR59</accession>
    <name>A0A8I6WR59_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR59"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0016840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25930" checksum="13A4BE245E0E2548" modified="2022-05-25" version="1">MLLCGYRWCPEYTVEEQYRDFNQSQYICTVRVFLDYPGAEEPIHWSYGLGVTVYMAVQDGAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEYPLLRSTVEMLENRDMDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGTRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WU94</accession>
    <name>A0A8I6WU94_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Trafficking protein particle complex II-specific subunit 120 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123427163</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044967071.1">
        <property type="nucleotide sequence ID" value="XM_045111136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WU94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0179460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0179460"/>
    </dbReference>
    <dbReference type="GeneID" id="123427163"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0148230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0148230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0148230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0179460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0179460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0179460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="202"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="343"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="548"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="7">
        <location>
            <begin position="858"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="8">
        <location>
            <begin position="1040"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1197" mass="130716" checksum="7A85DC9BC16420A7" modified="2022-05-25" version="1">MEPGLSIESGSAIRVAVLPVGGPIPPQCLRDYAALVARHARVDLASLRPYYSEHQKSPFAHQPWDTGCLRLKFVLGGCVPSPWEDFQSSRKVLAVVGICHLPSSPDLARVAADFLDAARTYPSALASRCFAFCPTDAQLLEERKDGIIMFPPSDQKSLELHMFTMIQDLAASLLMEFEKWVLRAESTGTILKTPLDSQSSLGSEEVIKAKKRRLGRAQKIIGDYCLLAGSPADANAHYTTAIDLARLTGDVFWHAGALEGSVCALVVDRMGQSDPVLEDEVKYRYYTIIQLYRRATLQDNAQRVSPVSFELEAALKLARYLCRREVAKEVSDLLMGAADGAKALIDASDRLILYIEIARLFGSLGYKRKAAFFSRQVAQLYLQQDNAYAAMSAMQVLTMTTSAYHVQSRKTSKPDHASLKELGASNSNADSGKAHSQSVVSLFESQWSTLQMVVLREILMSSIRAADPLTSWSAAARLLRSFYPLITPAGQSGLASSLSNSADRLPWGTRCADPCLPFIRLHSLPFHPSQRDIVKRNPHKKEWWIGAGPSGPFIYTPFTKGGTSGNSKQEINWIVGEPVQVMIELANPCSFDLVVESIYLSVHSGNFDAFPVTVNLPPNTSKLVLLSGIPTQVGQVSIPGCIVHSFGVITEHLFKEVDSLLLGAAQGLVLSDPFRCCGSSKFKSVNFPSISIVPPLPLLVANVVGGDGSILLYEGEIRDVLITLTNAGTVPVEEANIALSGKNQDSVISIAHSTWKSALPIKPGGEVTFKVTLRAWHLSLTDLEADGSRSPANPRRVAREGINPFLNIHYAGPSADQGNGVVSLPPGRRLAVPLNICVVQGMRLVRARLLSMEIPARFSEAHLRPVSGKDNISDESNMLHNDISLLKIDPYKGSWGLRLLELELFNPTDVVFDVDVSVHLDGTIVPEDNTADVACHKTRIDRDYSARVLIPLEHFKLPVLDASFFVKENGSDEQLGSKVTIAEKNAKAELNASISNLISKIKVRWHSGRNSSGELNIKDAIQAALQASILDILLPDPLTFSFRLAKDAKPANDSSHSTVENVGPFTGENVLRCKDPISAHKMTHMEVQIRNNTKEIIQMNLSISCKDVAGENCFEENSATVLWAGVLNDIQLEVPPLQEVMHPFSVYFLVPGDYSLQSSSVIIDATDVLRARAKAESPDEPILCRGSPFHIHVVGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUI2</accession>
    <name>A0A8I6WUI2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123444774</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977547.1">
        <property type="nucleotide sequence ID" value="XM_045121612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUI2"/>
    <dbReference type="SMR" id="A0A8I6WUI2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298980"/>
    </dbReference>
    <dbReference type="GeneID" id="123444774"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0249240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0249240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0249240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0298980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444774"/>
    <dbReference type="OMA" id="NACPRLP"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="516" mass="55843" checksum="211D26ABD0570190" modified="2022-05-25" version="1">MESGEPLPRHDGRGGWRAALFIVAVGFLERIGFTGVGGNLITYLTGPLGMSTAAAAAGVNAWSGTVLVLPLVGALAADSRLGRYRAVLIAGVLYLLSLGMLTVSSMLQTSHLRPAGCHNTTSTCSPPPSASSPARIAFFYTAVYLLALAQGFHMPCSEALGADQFDPSLSASRSSYFNWFHFSISWGYAIAAIVITYIEENVGWTVGFAVCWATMVVYLVVFLLGSRTYRVEQPVDSSSLAQLTKKLIFSRADATDTQRLLATGQNMDDNGFLGKLLPIWLSSIVIAAATSQVSTLFTKQGSTMDRRLGAATGLVVPPAALQCFVSFTYIALVPVYDRALVPLARRLTRHPAGVTMLQRIGAGMVMSCVTMVVAGLVEAKRLRVATDAGLLDRPDVAVPMSLWWLVPQYVLVGFTEVFCFIGLEEFFYDQVPDGLRSVGLALCLSIFGVGSYASGMLVWAVDWATTRGGGESWFADNLNRAHLDYFYWILAGLSALQVAVFLYFANRYVYRKKPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X162</accession>
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        <recommendedName>
            <fullName evidence="2">VTT domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC123448559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X162"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0371680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0371680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0371680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0309400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0309400.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0371680.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0371680"/>
    </dbReference>
    <dbReference type="OMA" id="WVQRNKY"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="entry name" value="VTT_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47699:SF1">
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    </dbReference>
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    </dbReference>
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            <begin position="50"/>
            <end position="71"/>
        </location>
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        <location>
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        </location>
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        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0371680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="270" mass="29222" checksum="E7FA75F5524E7D53" modified="2022-05-25" version="1">MARTTNGWARGVVAFVAVAVAVSAGRRYSWDVEAAVAAFRRGRGSLGPWAAPAYVAAHALTLALCPPYAIFFEGAAALFFGFLPGVACVFCAKILGASLSFWIGRAIFKYFASAMEWLQRNKYFHVVVKGVEKDGWKFVLLARFSPLPSYIINYALSATDVGFFRDFLLPTIAGCLPMILQNVSIVSLAGAAVASTTGSKKSVYSCLLPILGIVSSILISLRIKQYSSALAIPEDLTNSPTNGNINEDAKLLWRNLKIPLPRKLGKEDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1T8</accession>
    <name>A0A8I6X1T8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123431159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970931.1">
        <property type="nucleotide sequence ID" value="XM_045114996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X1T8"/>
    <dbReference type="SMR" id="A0A8I6X1T8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208240"/>
    </dbReference>
    <dbReference type="GeneID" id="123431159"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0173120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0173120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0173120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0208240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431159"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF130">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="173" mass="19389" checksum="2239C1B6EA92DDFF" modified="2022-05-25" version="1">MASRDPLIVGRIVGDVVDYFDMAAQLRVLYSNREITNGFELRPSQVENQPTVRITGRRGSLYTLVMVDPDVPSPTNPTQREYLHWLVTDIPDGGDMSRGTEVMAYEKPQPTAGIHRVAFVVFRQAARQAIYAPGWRSNFITRDLAECYGLGAPVAAAYFNCQREGSCGGRRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X290</accession>
    <name>A0A8I6X290_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SRP54-type proteins GTP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X290"/>
    <dbReference type="SMR" id="A0A8I6X290"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0114710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF39">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="331" mass="36377" checksum="D10B52AAEE9E7FC7" modified="2022-05-25" version="1">MESDPVKIAVEGLEKFRQEKSDLIIIDTSGRHMQEAALFEEMRQVAEATKPDLVIFVMDDSIGQAAFDQAQAFKQSASFGAVIVTKLDGHAKAGGALSAVAATKSPVIFIGTREHIDDFDVFNVEPFVARLLGRGDLPGLIDKMESIVPADQQSDLVAKLSKGAFTLRLLYEQFQNLLKMGPMSQIFSMLPGFSSELLPKGQEKQSKEKFKRYMTIMDSMTPAELDSMNPKLMTESRIIRVARGSGRKVKDVMEMLEEYKRLAKMWSKLNVSKLIPPNGKMSAQAIQKMLKVMPPQVVQQMGGKSGLEALLKQLGGGKDTSKMLAGMRGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3X0</accession>
    <name>A0A8I6X3X0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444545</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044977239.1">
        <property type="nucleotide sequence ID" value="XM_045121304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3X0"/>
    <dbReference type="SMR" id="A0A8I6X3X0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292830"/>
    </dbReference>
    <dbReference type="GeneID" id="123444545"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0243530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0243530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0243530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0292830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444545"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097466">
        <property type="term" value="P:ubiquitin-dependent glycoprotein ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000015">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035175302" description="alpha-1,2-Mannosidase" evidence="9">
        <location>
            <begin position="36"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="675" mass="74465" checksum="EAC9EFF327A75403" modified="2022-05-25" version="1">MMQPMRLRSQGRGSPLLCLSAALLAVALLPGAAVADGVTPTEALRLRDEVKDMFYHAFDGYMQHAFPLDELRPLSCQGEDSLGGYALTLIDSLDTLALLGDKERFAAGVEWVGKNVRFDINKTVSVFETNIRILGGLLSAHLIASDYATGMKIESYDDQLLHLAADLAQRLLPAFDTPTGIPFGSVNLLYGVDDDESKITSTAGGGTLTLEFGVLSRLTNNTVFEQVTKNSVRGIWARRSKLNLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLLKAYLLFGDEEYLYVFEEAYKAAMHYLHHDPWYIEVNMNSGATVWPLFNSLQAFWPGLQVLAGDVDPAIRTHAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDFRYLDVGRDILASLQYAAKCPCGYCHISDVETHKQDDHMESFFLAETVKYLWLLFDLAVGPDNIVENGPYKYIFSTEGHLLPATPEISLVDEHCLYSEAFCNNSTDRGYISADGSSKHPSVNCTHFDERQTVSSQNSLSNVLSTTRGYIKGVCPGLSHAQKLGISYHDNTVEQNSRSHENPDESYTDQSSVQIQSSSVILISHPATSPPDEPLETGSRTQYLENIVVTGDSEPVEVSGSSEGSTENLAEDTTLGISEQIEVTAAEEVASQDIHIEDGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X5T2</accession>
    <name>A0A8I6X5T2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123443657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976070.1">
        <property type="nucleotide sequence ID" value="XM_045120135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X5T2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0263560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0263560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0263560"/>
    </dbReference>
    <dbReference type="GeneID" id="123443657"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0263560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0263560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0263560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46951">
        <property type="entry name" value="BED-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46951:SF3">
        <property type="entry name" value="OS01G0547200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="8">
        <location>
            <begin position="62"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="990"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="1158"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="1161"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="437"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0263560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6G1</accession>
    <name>A0A8I6X6G1_HORVV</name>
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        <recommendedName>
            <fullName evidence="8">RNA polymerase II subunit 5-mediating protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123445318</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045122281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6G1"/>
    <dbReference type="SMR" id="A0A8I6X6G1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0312420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0312420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0312420"/>
    </dbReference>
    <dbReference type="GeneID" id="123445318"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0261010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0261010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0261010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0312420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0312420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0312420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445318"/>
    <dbReference type="OrthoDB" id="21413at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001243">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Prefoldin_URI1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prefoldin chaperone subunit family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0312420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="40834" checksum="C165330BBC8861A2" modified="2022-05-25" version="1">MATARKGTATPLASAFSPEETRRAVSRVSQAIADRRADLARVQGFFADNAALVNLVQRLPDELSHQIMVPFGGAAFFPGSLIHTNELLVLLGDGYYADRSAKQTTEILHRRGMELEAQMEAIKATISDLEAEAKFFESTAAEASEGLVEIREEYDEEDTEIVSSKTEASSSSGGISDEEHARMMARFDELEMLEKEAGSSSEDEGNDDDDDDDEDEDAGSSEDGEENGDTLSYGNEHGNVSFGASVSGSCGNDQSQGNAQLKSALKKLGEKKALQGSSLAPSGSTSITNSEIQASLRKAVSFKDENGQIVCSSRYSSGPKVSSSRDRKILPGGQKAFTGSIVEHDDGLSAIEQPRSNDSEKAASSASSSRPMSRFRMQKGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6M2</accession>
    <name>A0A8I6X6M2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6M2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF27">
        <property type="entry name" value="TTF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416980.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24752" checksum="D2B3239DDADA5EFD" modified="2022-05-25" version="1">MRKYQSGAYKRKKKAEEQNLINRSYKGGMDKFVRKEAPQVSSGNQSVDPSILAIVPYNDATNGQTETYNMEVEEDHIDVNLNNSPREDVDDSSKSDIFDPRNWDSLDSKQIDILAQKGPKRDNSIKKGPKDRYRKRFTTIFYTRFLSNGECCDRDWLVYSKQLDRFFCFACNLFRKSHQKGQLANEGCNDWSHMTNRLKDHETSADHVLSMTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7H1</accession>
    <name>A0A8I6X7H1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123444273</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976885.1">
        <property type="nucleotide sequence ID" value="XM_045120950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7H1"/>
    <dbReference type="SMR" id="A0A8I6X7H1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284940"/>
    </dbReference>
    <dbReference type="GeneID" id="123444273"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0236370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0236370.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0236370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0284940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444273"/>
    <dbReference type="OMA" id="NHGIDPW"/>
    <dbReference type="OrthoDB" id="411581at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000329">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001812">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g29760, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF379">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035233105" description="DYW domain-containing protein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="537"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="629" mass="68750" checksum="6732D66ED31B1AED" modified="2022-05-25" version="1">MAPLPRRLVPLRDSATLLLLPSLVAASSQSQFRLREIHAHLLVSGRLASPSHHADFVASLASSSHLSYARLLLPQRPATLLAHNGLLRALARGPCPGLAFAAFRELPLAPDHYSFTFLVRAATSLAAAASATPVPTDVAVNLLAGSVHAAAFQHGHATDPHVQSGAVSMYAAVGDVGAVRAAFAEIVSPDVVCVTAMLGALSAGGDVDTARELFDGMPQRDHVAWNAMLTGYVRVGRSREALGLFDEMQKAGVAVSEVTLVSVLTACAQMGALERGMWVHSYVCSRGMRVSVTLGTALVDMYSKCGVVTMSMEVFETMRERNIYTWTSALSGLAMNGMGEECLELFKRMESAGMEPNGVTFVAVLRGCSVAGLVEEGRACFDSMKDKHKVEPWLEHYGCMVDLYGRAGRLDDAVDFINSMPVEPHEGVWGALLNASRIHNNVDLGKHAMHKLTEIESKNDAAHVLLSNIYAESHNWKGVSKVRNMMKSKGVKKMPGCSAIEVDGKVHEFFVGSKSHPRYKDIQTMLAEMSHRLRLQGYAANTKEVLFDIEEEEKEGAISLHSEKLALAFGLITLPEDTVIRIVKNLRVCKDCHDYTKLISKVFDREIVMRDRNRFHHFKHGACSCRDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9J2</accession>
    <name>A0A8I6X9J2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123428570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968727.1">
        <property type="nucleotide sequence ID" value="XM_045112792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9J2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0217580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217580"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0217580.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217580.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217580"/>
    </dbReference>
    <dbReference type="GeneID" id="123428570"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0181010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0181010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0181010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0217580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0217580.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217580.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214:SF12">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214">
        <property type="entry name" value="ZINC FINGER, RING-CH-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="113"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0217580.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="374" mass="40745" checksum="B808A5718957E6FE" modified="2022-05-25" version="1">MGVEEEQDVQQPDGAAAKPDAARSPTTPEHGQQKPSNPRVSTCSSDKDDGLALCRVCHCVEPDLRGESALGFLGIVPPSPDPSCLKADKDPDNDATKNSTSKDGIDVPKFLEFISPEGEIFKCATDIESGPLRPQDDVVNLGCSCKNELALAHYACALKWFISHGSTVCEICGNVATNVRPEDFNMVLASLKDYEALRERTSTGDLSYLHYRADASVDPVALAAVRRQRLCEISSWFNPHNTHFVFSRSQRHNNNNNEEVPVSPSNNSADYSVSAWGPSHGGSTVGSTGAFVAIALAFVILAWFVAPHVGKRAAAIILHMLLGGLCSLTIIISLRFVFPRIQFGSMRYWAILFMSWFLVFGVWASRTRTIRSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9U3</accession>
    <name>A0A8I6X9U3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123430485</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044970289.1">
        <property type="nucleotide sequence ID" value="XM_045114354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9U3"/>
    <dbReference type="SMR" id="A0A8I6X9U3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178940"/>
    </dbReference>
    <dbReference type="GeneID" id="123430485"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0148010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0148010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0148010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0178940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430485"/>
    <dbReference type="OMA" id="HKPAVPN"/>
    <dbReference type="OrthoDB" id="614159at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF760">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C 42-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="14">
        <location>
            <begin position="35"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="340"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="386" mass="41882" checksum="08866B46F3884C35" modified="2022-05-25" version="1">MTMKNAWQANSAMSTISREPVLSKTTERGENDRLEYAVSSMQGYRANMEDAHAAVEDLDVSTATSFFGVYDGHGGPAVSMYCAKHFHLEVQKHPHFNDSLRIAVESAFFRMDQMMMTEEGRRELYEYSPANNNANANSTVKDMLLSCACVNLKKRPGPADVGSTACVALIRDNQIIVGNAGDCRCVISRNGQATVLTNDHKPSVPAEKRRIENAGRSVTVTGGAGRIDGGIAVSRSIGDMRYKSNSRLTPALQALTCSPEIRLENITAETEFLVMACDGVWDVVLTQGLVEIIRKNMKSGMDLGKNCEAILDMCVEPPQPSVDNMTILLVRFKHKEAAAAAAAPDPSRADDGKHKSKASVELGVTDNLNKAAPPTASARAKPRRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBI8</accession>
    <name>A0A8I6XBI8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SOUL heme-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123444885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977698.1">
        <property type="nucleotide sequence ID" value="XM_045121763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBI8"/>
    <dbReference type="SMR" id="A0A8I6XBI8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0301820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0301820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0301820"/>
    </dbReference>
    <dbReference type="GeneID" id="123444885"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0251770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0251770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0251770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0301820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0301820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0301820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444885"/>
    <dbReference type="OrthoDB" id="6424451at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000010">
        <property type="entry name" value="SOUL heme-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000013">
        <property type="entry name" value="Soul heme-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF58">
        <property type="entry name" value="SOUL HEME-BINDING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0301820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24471" checksum="B07849445E94F9B5" modified="2022-05-25" version="1">MGLAMAKLMVGKIIVETPKHEVVHTGDGYEIRKYPPCVAAEVTYDPKEMKGDRDGGFQVLANYIGAFGKPQNTKPAEAIAMTAPVITSSSSGSGEAEPIAMTAPVITSSEPEPVAMTVPVLTAEGREEQTGKVTMQFLLPSKYAKAVEAPRPTDERVVLREVPERKYAVVRFSGLAGDKVVAEKAEGLKAALHKDGHVVTGPFVLSRYNPPWTLPPLRTNEVMFPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDY6</accession>
    <name>A0A8I6XDY6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDY6"/>
    <dbReference type="SMR" id="A0A8I6XDY6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0168800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0168800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0168800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0202930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="57"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="233" mass="26387" checksum="2CABBB7F09AFC357" modified="2022-05-25" version="1">MIIMASRTKFQTHFSAGKAQFPSEPMARWRLVLTVALLAAAGWRADALSVKVTGTGTECVHELAPYEGDSVLGYFVAHRDIDLTVTSPGGNTIHTSKGKSSDNIEFKAPSGGIYKFCFHNPCGAPETVSFYIHIGRIPNVHNLAKYGHNLVLLEALEEEAMRKRFKEWMDRYHRKYKDEEEKAQRYELFKKCAEKVDKLNALPDGVTYGTNHFCDRSEEEMQPYFTGELDVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFW6</accession>
    <name>A0A8I6XFW6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFW6"/>
    <dbReference type="SMR" id="A0A8I6XFW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0285280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0285280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0285280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0341840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341840"/>
    </dbReference>
    <dbReference type="OMA" id="HQLSTEY"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="12629" checksum="F1DF506218E6A3D5" modified="2022-05-25" version="1">MFKSVPPAQAVLIKLVLHHWSDDDCVKILTQCKKAIPWREEGGKVIVIDIVVVDSSSGPTHEAELLVDVAMMVSTNGRQRDETDWSELFTKAGFSDYKIVKKLGARCVIEVYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJ80</accession>
    <name>A0A8I6XJ80_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJ80"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0307560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0307560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0307560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0369430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="106" mass="11791" checksum="96DFC96B356F973E" modified="2022-05-25" version="1">MGATIPARIQPCYIDHVPCPKVVSSLGASFFGFTIKPDAAPFRIIIYRPFFPPVPSRMKIVVCMFDFGCRCNRIGGSILWQDQSTGQTKTLLQEELCYAPPPDIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJG3</accession>
    <name>A0A8I6XJG3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJG3"/>
    <dbReference type="SMR" id="A0A8I6XJG3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0203550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0203550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0203550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0203550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0203550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0203550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF92">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="235"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0203550.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="418" mass="46897" checksum="6DC49A4548C072DE" modified="2022-05-25" version="1">MPGGRTAHSRFKIPLSIEDGASCSFTKQSGTAKLLRMASLILWDEASMTKRQAVEALDNSMRDIMGRRHQPFGGKTVVFGGDFRQVLQVVRKGSRGQIIDATLRSSYLWKGMRQLRLVTNMRAHNDPCFADYLLRVGNGTEETDEEGNIRLPQDICVPPTGDGIDLENLIDHVFPAPDHNMSDPNYMTSRAILSTKNDNVDKINTCMIERFQGEEKIYHSFDSAEDDPHGNYAPEFLNNLTPNGLPPHALKLKIDCPVILLRNIDPANGLCNDTRLVVRGFQRNVIDAEIVLGQHAGRRVFLPRIPLCPSDDDIFPFKFKRKQFPVRLSFAMTINKAQGQTIPIVGVYLPDPIFSHGQLYVALSRATAKRNIKILAIKDDGKGKVKGKEQSKNSKKRKRSEPLLTTMKNIVYKEVLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLU2</accession>
    <name>A0A8I6XLU2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLU2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0261080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0261080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0261080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0261080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0261080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0261080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0261080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="16072" checksum="651348A20021AA33" modified="2022-05-25" version="1">MVYGKACHLPLELEHKAYWAIKELNFDFKLTGEKRLFDISSLDEWRTQVYENAQLFNEKVKRWHDKRIRKREFNVGDYVLLYNSRLRFFAGKLLSKWEGPYVVEEVYRSGAIKINNTEGNCPRVVNGQRIKHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM81</accession>
    <name>A0A8I6XM81_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Cysteine-rich receptor-like protein kinase 25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XM81"/>
    <dbReference type="SMR" id="A0A8I6XM81"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0524170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0524170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0524170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0435700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0435700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0435700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0524170.1">
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    <sequence length="688" mass="74329" checksum="00DA7BB6F6325264" modified="2022-05-25" version="1">MPSVKVKPPSTAPHMLLLVLLAASLGASQAWVQCGPSAASPSPAPAPAPSPASSTTANSSVFRANILALLDELPHATAPTGFASLSRGTGSDRAFVRGLCRGDLAESVCLADLQEAVRNLSGRCASSRSAGAWYRQCYISYADTNDSAVYEGRIGGILYDTRRVADADSYQRTYYALMSRLVARAAGGGNRSARTSMFATGEAVYAGDDPNGTMYGLVQCMRDRSDAECQQCLQSLVPKLPTCCRGYQGGVAMDFNCHLRVQLYTYYDLALDAPPPAPALPPPSPPSTGENPGKGRPSKRAVVVAATLTTGTLLLVLLLVLLCVYMKRRADKRPRGGAREDSSTTYVSSEQFTLPLLRAATGNFAAENKLGEGGFGQVFKGKMPNGQAIAVKRLSQSSSQGFHELKNELVLAAKLGHRNLVQLLGVCLEEKEKLIVYEYLPNRSLDTLLFDNGRRRRRRHGLDWRKRHTIICGLARGLLYLHEESRLRVIHRDLKPSNVLLDEHMNPKISDFGLARAFRGDQSRDVTKRAAGTLGYMSPEYAYCGHVSVKSDMYSFGVIVLEIVTGRRNSSAGQDQAAASSLLSEVWEKWRAGTAAEMADASLGGQYPRPQLLSCVHIGLLCVQKKPELRPDASEVVLMLSSQSTSRRAPSRPAFYAGSTTGAVAASSARRCENVSRNGVTMSELEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XN49</accession>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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            <strain evidence="9">cv. Morex</strain>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="term" value="F:histone reader activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
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        <property type="entry name" value="OS12G0161500 PROTEIN"/>
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    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
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            <begin position="786"/>
            <end position="910"/>
        </location>
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            <begin position="356"/>
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        <location>
            <begin position="618"/>
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        <location>
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        <location>
            <begin position="715"/>
            <end position="725"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
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        </source>
    </evidence>
    <sequence length="1052" mass="115920" checksum="0C1088337446D7ED" modified="2022-05-25" version="1">MDDVCDECGDVGYSHLLLRCSNCNNAARHRYCLDEINFDLAVEWLCSDCIPKHSEAIKSPGETNCQRQLRNTQLSFFVVNEPNVEHEKATKAQGLYRSRLCRKREETLDSGEHVPSGDTSNRGRCVEETLDSCEIFVSDIPNHIDCKKEGEDINGHLICAPESYGGSSNFALDHASSIEPNNLQKAIGGSKLASGYVDCHDLPNARSGCFASSKYVEGFFPRGRKGDMFSLMNDVEGSCPMIVDKSCSTSACMEQADGLSVNMEKSEPSKLVKGLEESVVAAKFAPNHSKPMQGSDLKKGSVDVLNPLEQRLDSLAMLQSSSSNELNDAAIQENGAERTRCFAGKETVASELGNHKGSNPSMANESLSSSQGNSDEANRRSDELLSSTKDNKMRRRLCVARKIINSCNVNGTTDSESARDAINLAGKTSSLAVKKVPLQLILQEKGINNELLPSKFLSPCESTKINPRKRKQSEDYAPDGTKYQEDCIISGIGNANVALSKSGRPLRACQYMPRKDNGSNDKAIGRSKMGKSKKMSAKSGRPLRACQYMPRKDNGSNDKVIGHSKMGKSKKMGKSWLANNGIRDSRKRGMVTGVLQPNSLRRRSVDRSRPSPYSLRRRSVDRSRPSENMDPALMESSCALNNPSSSCAAQSSGFSSKTEELWEGDEPKKRRKLILTYDEEEDAEALQAEDLNSRSCDDDEHVKKQRKCMENAAENQRRSVKEGKEALGSGNLNHQYSKNRKQVKKQRGIEAEEDENASVRNPNTECARMPVISECIGGQPLDIPYWTGIMKVNNNYIPLAAHLSTKAGKKVQELSRSLPPIMKVAKLSTSKSCPKHLEAPIPTADSIGLYFFSGDMRPNKELDELVKHLADSGIILEAVVGLAKLFVFPSAVLPAEYQTFQGKPYLWGVFKPRKYKIRRLPPVEQDCMAHVSKEEHAQEQHALDQEDKARTDTLDHVVHYENQPLLDANQQGKEALSGNGLTPVDVQALVCANIGPADHRQPCSNPEAPPLELCGFVVSRTPRSAQLIQEMQKEGALLFVVQQVITEPGSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRY4</accession>
    <name>A0A8I6XRY4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRY4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0419940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0419940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0419940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0349520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0349520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0349520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0419940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0419940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0419940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0419940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="13048" checksum="9B5ADFE078BCE815" modified="2022-05-25" version="1">MCGLSCLKFQRRSRIQGGGEGREAKSSSFVPGSPFPCEREIRPPPAEMQAAALWSKGLPGLRSHLCAAASFHSMPAFPPSGRASSIPSMNMGNRSYPRYAWTHLSVVIWTGWEHERHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS76</accession>
    <name>A0A8I6XS76_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS76"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213150"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF19">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="20427" checksum="60D9357016E504FE" modified="2022-05-25" version="1">MAEESRCFKFCMIAMAIVIMAGFVFSIVWETTRPCDTKYTVTITSVDGLDLHSDRQALSPVFGITVHIDSTHNQMTNECVGGVGSSAIVTYGDALLAKGTMPGLCVQRTKVREAATKAWGMNVEVPHFLRDRLASELERGQAVVDVAVRTPGGGGCYINGCLDTVLVCKAKIGGGPSPCFRATTVSGTNTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTF7</accession>
    <name>A0A8I6XTF7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTF7"/>
    <dbReference type="SMR" id="A0A8I6XTF7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="184" mass="21901" checksum="51699481C39197CF" modified="2022-05-25" version="1">MRGYLQGPFPYVTYFTYLYHVVFDAPLCVILLTMSYDACFVGMAASQPNKAKAEWGEEKDAASEEQLLMDRAAKKLREEDAAEARKKKDEEDNAKRDEEWQMFLEHKRYEARIKENDRKTLEKRKKEYEANFKKMLAEAATKREREHQRFTERVKEEASKLRKREEEEEEERKKKKGKGPCSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XV18</accession>
    <name>A0A8I6XV18_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123441024</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973490.1">
        <property type="nucleotide sequence ID" value="XM_045117555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XV18"/>
    <dbReference type="SMR" id="A0A8I6XV18"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0323380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0323380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0323380"/>
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        <property type="entry name" value="matE"/>
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        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
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        <property type="entry name" value="MatE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="458"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0323380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="494" mass="52648" checksum="980496304D29CFEC" modified="2022-05-25" version="1">MADDAHGPSGRLESILTDSSAPLASRVWAAGAIELGLLTRLAAPAVVMYMINYLMSMSTQIFSGHLGSLELAAASLGNTGVQMFAYGLMLGMGSAVETLCGQAFGAHKYDMLGTYLQRSAVLLCCTGIPLAVIYAFSEPLLVLLGQSPEIARAASIFVYGLIPQIFAYAINFPIQKFMQAQSIVLPSAYISTGTLVLHLLLSWVVVYKAGLGLLGASLVLSLSWWVIVAAQFAYIAVSPTCRETWTGFTCQAFSGLPAFLKLSAASAVMLCLETWYFQILVLIAGLLPNPEVALDSLSVCMTLAGWVFMVSVGFNAAASVRVSNELGAGNPKSAFFSVWVVTALCAAVSVAFAIVILCLRNHISYLFTESEIVSDAVADLCPLLAITLILNGIQPVLSGVAVGCGWQQFVAYVNIGCYYIVGVPLGIVLGFVFNLGVKGIWGGMIGGTTMQTAILLWVTIRTDWNKEVEEAHKRLNKWDDSKKEPLLTAGTDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XV87</accession>
    <name>A0A8I6XV87_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123399479</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949826.1">
        <property type="nucleotide sequence ID" value="XM_045093891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XV87"/>
    <dbReference type="SMR" id="A0A8I6XV87"/>
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532120.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123399479"/>
    <dbReference type="OrthoDB" id="636220at2759"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Expan_Lol_pI"/>
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    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
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        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
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        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342088" description="Expansin" evidence="6">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="7">
        <location>
            <begin position="45"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="8">
        <location>
            <begin position="169"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="254" mass="27697" checksum="A8405835EDA0FB0A" modified="2022-05-25" version="1">MAGRMCFLQLFTVVLAFCFMPAKSGYWLPAHATFYGGADGSDTMGGACGYENLYNAGYGINNAALSTALFNNGLSCGQCYLITCDTSKSNMCKPGTSITVSATNFCPPNWALSSDNGGWCNPPRVHFDMSQPAWENLAIYRAGIVPVLYQQVACQRQGGLRFTINGFNYFELVLVTNIAMSGSIKSMSVKGTNTAWIPMSQNWGANWQCLAGLKGQGLSFAITSSGGQYKVFQDVVPAWWLFGQTFSTWQQFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWG3</accession>
    <name>A0A8I6XWG3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWG3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053198">
        <property type="entry name" value="Gynoecium_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066">
        <property type="entry name" value="OS05G0427100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066:SF45">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="34"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="26662" checksum="D1C0C7A8AE65DC04" modified="2022-05-25" version="1">MAAAAPEASGSGSVSSAARLRMMCSHGGSFAPCGPDGAVRYVGGQTRVLAVPRGVLFRELATKLGEMAGGKIVKALRYRLADDEEVLVSVTCDEELAHLRDEYDRLQATRPSATFRVFFSGVQRPQASGRPPLHPGTLRRVQSDPCTAARVAAGPAPPPMRRVQSAQDVAGYGRFKPRCHEKPHPRRDRYAPMPAQPTRSPLSAMSKNSHGAIRTGQELMTTTKKARSRDAVAAEAAALYGSWSEIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0T0</accession>
    <name>A0A8I6Y0T0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0T0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0640870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF189">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
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        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="6">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="188" mass="22006" checksum="E750D4995D9DE5D9" modified="2022-05-25" version="1">MPSRRRSASGYRGVRQRPNGGFYAEIRSGDLRLSLGTYDTAHEAARAFNAAAWRLGRPRLQMNFPDVHTLQQALDLAPPPRLNSTQDRAEHTALQRRLLVAQEDERIMAEWRRRHPEDVAYEQEYWERRREERLDRRRRKALACAQADLVNAGGRSFFSEEDERWFDIWLSTSDDTKDDDDGADDWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4U3</accession>
    <name>A0A8I6Y4U3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123399522</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949858.1">
        <property type="nucleotide sequence ID" value="XM_045093923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4U3"/>
    <dbReference type="SMR" id="A0A8I6Y4U3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533630"/>
    </dbReference>
    <dbReference type="GeneID" id="123399522"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0443930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0443930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0443930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0533630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399522"/>
    <dbReference type="OMA" id="YYWTSAT"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035341376" description="Expansin" evidence="6">
        <location>
            <begin position="26"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="7">
        <location>
            <begin position="47"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="8">
        <location>
            <begin position="171"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="255" mass="27537" checksum="FE129DA604FA1382" modified="2022-05-25" version="1">MGKQTAVALVLLGVLCGIASHGVDAQYYWTSATATFYGGADGSGTMGGACGYGNLYGSGYGINNAALSTALFNDGAMCGACYIIYCDTGRSKMCRPGTQITVSATNFCPPNWALPSNNGGWCNPPRLHFDMSQPAWTSIAIYQAGIVPVMYKRVSCQKRDGIRFGISGRDYFELVVVTNVGGSGVVAQMWIKGSNTNWLAMSRNWGANWQSNAYLNGQRLSFRVRLDDGREATASDVAPSNWWFGATYSSWINFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6A1</accession>
    <name>A0A8I6Y6A1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6A1"/>
    <dbReference type="SMR" id="A0A8I6Y6A1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0510970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510970"/>
    </dbReference>
    <dbReference type="OMA" id="QWCIADQ"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF35">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237249" description="X8 domain-containing protein" evidence="8">
        <location>
            <begin position="41"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="9">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="133" mass="14978" checksum="2E3F1664497B902D" modified="2022-05-25" version="1">MRTYLPPSAGRVKNCDLRNQAATRLMLLLYFILHFAAVSAQQEDGNWEKQWCIEDEQRPDGILQIALSWACGPGGADCTMIEPNKSCYLPNTVRDDASYAFNSYWQKYKKHGGSCYFNAAAMVTNLNPSMSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6Q3</accession>
    <name>A0A8I6Y6Q3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore complex protein NUP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6Q3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.150.10.10">
        <property type="entry name" value="Serralysin-like metalloprotease, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416:SF20">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1069"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1211" mass="122402" checksum="CEC6B7A3F6A2AD8B" modified="2022-05-25" version="1">MAIDHSPPADVPIRDVNSPANLAKQYMSSRYSREPQQSNLRSQVLFGNKAEASNTGYYMTSGAPLVQELNQVNNENPGLPVNGYMASGLRGRSAICKMSRSPFFKGPSSSSDMNVSPFSLSHARTPSLAAGGRQVLKRRGAEFDNELGSVGPIRRIRQKSNMMSPFKDARASPRGNFLPTRTIGSDFAEGSSALQEPSSSKRLLLESGQSLRTVERQRYSEDGKLSNDDVPPVPTQPNKMAEKIFEQLNIIAPSPKGKQSGQQSVAGKPRQSMSNGPKGVSDPSSSRQFQDSDGINHPHDSDLNGSTLNEDKLMKDGSSKVHSNTFHQDLGNKEMKSDHVAILNKPAISGNSFSATPSRKPGFKMAVFEDLTELDDDLEAPVPSKNSKFETDVKATEQKFDSKIKEQKVQHVKFEKKVESTSSKENVINSSVTEQPRTSTSKDVSTPGLFSFSDPEKKGTPNVSSNNNSGFTFPHVPPADSPEATVSAVPLASNKDDKQTSASPFMFGAKPSSTSDLETSTTAGVKTKGRPGESATKPASLDTPNLEKGNGRGGAGDIHKSSDKVLPTAAPTLTPPLHFASAASASPGLSNGLSHTSPPKLSDTTPTHIPAVNAASLTTIAGFSVSSSSPPVSSSLPAFPTINFGSSTSTVAAPKPESTSTETKPASTSLFGTVGTTAESKSKAPEPASKASSNLNTASVFSSNITTFSSSPITSSSTSSSTATFSSSPVASFSVGTAPATSTPSTTSGVQPASTAAFAFSSSGNSIFGFSSPAQSTGLSTTVGGSTSQPFPASTIFSSKLPQPQGTVSQPPQSSATQFSSSFPIVASGAAASSSAPGTVSFGVGASPPGTLLFGAGSSSSGPGTVSFGAGASSSGGPGSVPIGVGASSSGTGVSSFGAGVSSSGPGTVSFGASLSSSGPGTMAFGGAPSSSGPGTVSFGVTTSSSGPVFGNSPFGSGAAAFSGSGSGFAFSSPSSSTASSFTVASTSMFSSSSTASSSAALSNPFGSASSSPSMFTFGQSAASSGGAFSFGAQSAPAFSSQAPPVYSFTSASASMNLSTPQPAFGMTNANTGFGMGSPGNDQMSMEDSMADDTNQAAPQQVSAPVFGSSVFGQPGGSPAAPVFGQPAGSAAAPVFGQPASSPAAPVFGAPAAQPAGAFQFGGQQGSVQQNPSFPTGGSLEFQGGNFSLGSMGGDKSNRRIIKAKRTQKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8S0</accession>
    <name>A0A8I6Y8S0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
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                <person name="Muehlbauer G.J."/>
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        </source>
    </evidence>
    <sequence length="230" mass="25649" checksum="253504B3E9A3019E" modified="2022-05-25" version="1">MLKAVIQDRVLFNGFINANYNPVVPLLNGETWHVITALTAFLELFYDATVTLSGVYYPTSPLMVHTLLDIASHLKAYEADGLLLNVVADMKIKYLKYWKKVPLLYALAFILDPRAKLEGYRSALNVLSASLSLDYTDDFNTARDSLYDIYAKYEQKYSGVRMQRPPPAPTAGKKRGAWSKIFASSSSSSSTAGSSSTSASSIPSYWWKYANANVWIFSTNESSFKCVSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9V2</accession>
    <name>A0A8I6Y9V2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9V2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0546780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0546780.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0546780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0658860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658860"/>
    </dbReference>
    <dbReference type="OMA" id="GIWSAFG"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658860.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="25921" checksum="3D8440E6FAB74C97" modified="2022-05-25" version="1">MPPSASNKKKKATVSSGGSLPDEMIMEVLLRLPVKSILRFRVVCRSWAALFSTEQFCNLHMATPKIAPPKLLFVSPTSRPGSTEVCSCSPSAPKDDLLFSLDSACGNSMQLVMPVPCHGLTLLFDAVAPAYYICNAATREVTRLPPFSHVFRRTSTGLGFDARTRMYKVVRLIKGSRNDVESVRCEVYTHEGGSGDCSSLAGGRLSEEYPLDCAGLQALLLIMRHCTIYHLCLQTDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBN7</accession>
    <name>A0A8I6YBN7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBN7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0596970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596970"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF290">
        <property type="entry name" value="OS04G0422900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="2"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="116" mass="13133" checksum="553BF8DB4228C86F" modified="2022-05-25" version="1">MHVPITISGEKVAPFRNRKGTLSHNVMVVCDFDINFTFISCGWEGSATDPKVLRSALNNGFPVPPGKFYLVDGGYANTPYFLAPYRVVRYHLKEFARGRRGPKNHTELFNHRHAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YEC3</accession>
    <name>A0A8I6YEC3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952601.1">
        <property type="nucleotide sequence ID" value="XM_045096666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YEC3"/>
    <dbReference type="SMR" id="A0A8I6YEC3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0621750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0621750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0621750"/>
    </dbReference>
    <dbReference type="GeneID" id="123402712"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0515770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0515770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0515770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0621750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0621750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0621750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000005">
        <property type="entry name" value="Transcription termination factor MTERF4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF24">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0621750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="491" mass="54973" checksum="9759B60B3BA0044A" modified="2022-05-25" version="1">MLPLARAALRRLGPAAAGDGSLPARLLLLAPLASKPSSSTPPEYQMPSVTWGVIQGRRERLVSRVLALDFLRSAGVTDPAGELEAVELPSSLDVLQERLDFLLRLGLSTDDLSAYPFLLACSLRKNVIPVLSYLEKLGVTRARLAAFVRAYPACLHASVAVDLAPIVKALRGLDVDRQDIPRVLERYPDVLGLKPDGTISTSVAYLVGILGVAPRDIGPMVTHYPFFLSMRVGTTIKPFCDYITSLGLPMRILARIIEKRPYILGYDLEETVKPNVEALLSFGIRKEVLPLMIAQYPSILGLPLKVKLAAQQYFFNLKLKIDPDGFARAVEKLPQLVSLHQNVILKPVEFLRGRGITDDDIGRMLIRCPQILLLRNELMKNSFYFFKSELKRPISELLEYPEYFTYSLESRIKPRYMRVASKGIRCSLDWFLNCSDQRFEERMRGDFIEGDAPGPSFTMGGKLQMPGNQIVSDDDNEDSDDEVLYRRTVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGY5</accession>
    <name>A0A8I6YGY5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123412507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961394.1">
        <property type="nucleotide sequence ID" value="XM_045105459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGY5"/>
    <dbReference type="SMR" id="A0A8I6YGY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0738850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0738850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0738850"/>
    </dbReference>
    <dbReference type="GeneID" id="123412507"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0612820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0612820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0612820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0738850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0738850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0738850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412507"/>
    <dbReference type="OrthoDB" id="610439at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34838">
        <property type="entry name" value="OS08G0142100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34838:SF7">
        <property type="entry name" value="PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035259213" description="Pectinesterase inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0738850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20422" checksum="1EC45548BC22A189" modified="2022-05-25" version="1">MAMAKIILLLVVLVPIRMAAARRDCPKVPLLGAVAACQKACGTKLMHDLCISMMRQGGADMSPSHMERATVYAILAAHSAGNSFDDTKLAASNQLSQNSSLSTTLRNAYEGCMNDYAPADSSIDHIADEALPSCRFKGLADEYTRVITSVEKCRVRLLSPTLVKTPLYPMVLADRNKAVLANLLGKLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YH56</accession>
    <name>A0A8I6YH56_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helicase MAGATAMA 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951074.1">
        <property type="nucleotide sequence ID" value="XM_045095139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YH56"/>
    <dbReference type="SMR" id="A0A8I6YH56"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554420"/>
    </dbReference>
    <dbReference type="GeneID" id="123401355"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0460520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0460520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0460520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0554420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401355"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18042">
        <property type="entry name" value="DEXXQc_SETX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002371">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF476">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="2">
        <location>
            <begin position="265"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="2">
        <location>
            <begin position="456"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="3">
        <location>
            <begin position="540"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="800"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="830" mass="91527" checksum="84B056DAB008688A" modified="2022-05-25" version="1">MAVDKPGGSGSGGGGASSSPASTTDRFLKIVLSWDYLRIVAASKGADKAKGLREVKNSYASVDEYLGVFEPLLFEEVKAQILQGRRNDGEGEEDEIKLDWQRGAVRTCAESEGFHKLSMLVSDGLRDIVSLSENDLLLLSKEKFEEGVNPTAYAFAVVEQRGGKDTLSLRTFVAGEIRNLNVAQPVKSLRLQRFASILSKPDSFLWILKMCSLSTILREYSGMHSVASHPFKDLILSASENNRDGDDQNRAWNVPQPLMDYLKANLNGSQLDAVNAGLSRRSFVLIQGPPGTGKTQTILGLLSAVLHSAPARMQTRGGFDVQKHGPELDIESKHANWMKASPWLIGANPRDMIMPVDGDDGFYPTGNDLKPEVVSSNRKYRAHVLVCAPSNSALDEIVLRVLQTGIRDENNNTYNPKIVRIGLKAHHSVKAVSMDYLMDQKQSGVASDGGRPGAGERDRLRASLLDEAAIVFSTLSFSGSAIFTRMTRAFDVVIIDEAAQAIEPATLVPLVHGCRQVFLVGDPVQLPATVISTTARKLGYGRSLFQRFQAAGFPVQMLKIQYRMHPEISVFPSKEFYEGILEDGEGLDKKRPWHSYSCFGPFCFFDVDGVESHLSGSGSMVNEDEVEFITLLYHQLATRYPELKSSSQVAVISPYRGQVKLLTDHFRSTFGDQSKEVIDVNTVDGFQGREKELVIFSCVRCNKEQSIGFVSDFRRMNVAITRARSAVLVIGSSSTFKKDKHWTNLVESAKERNRYFKVPKPFTAFFAEDNFKTMKVERPVLDARISEAIEAINEVVARQEVMDADDAGDHQADGDDYDAMEADDGGGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJW7</accession>
    <name>A0A8I6YJW7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11654" checksum="0365CA93909966B8" modified="2022-05-25" version="1">METETEAKLIIRRVCMETETGICNLPADCLALVAYLTSPGDACRLAATATADSDEVWESFLPADILARCSTAGRRREDETKKELFSRLCDSPVLLDGGKLVIFAVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQK7</accession>
    <name>A0A8I6YQK7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC123407103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956085.1">
        <property type="nucleotide sequence ID" value="XM_045100150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQK7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0724560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0724560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0724560"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0724560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0724560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0724560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407103"/>
    <dbReference type="OMA" id="WAYARII"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0724560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="77" mass="8919" checksum="D5C764B03C49CA35" modified="2022-05-25" version="1">MVPPGARRWAYVRIMAGTILGGGLGFYVMHRIETSYKAKMEERLRRYEAHLLAKEKEAQELQDEGRREDQAQLLPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQV0</accession>
    <name>A0A8I6YQV0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQV0"/>
    <dbReference type="SMR" id="A0A8I6YQV0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0715240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715240.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="246"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715240.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35656" checksum="3408389C5F0D9AE4" modified="2022-05-25" version="1">MIDILSGTRYKSRQFGVLSDVLDHMKRHGTRSVPVEDLLGILRAYTEKHLANLRKLAKKRRVRMRTPPETDALNILLDAFCKCGMVREAETVFGRVRKKLQGNAETYNILFFGWCRARDPKKAMKVLEEMIQMKHTPENFTYIAAIDSFCSAGLISEARELFEFMRTEGSKISSPTAKVYAIMIVALGKADRMDECFELIADMIKRGCMPDVSTFKDLIEGMCLVDKLDSAYCILEEMGKAGFPPDIVTYNCFLEVLCSLQKADDALKLCERMIEAHCEPSVHTYNMLMVMFFAMREPHRALDIWIEMDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YV77</accession>
    <name>A0A8I6YV77_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123398356</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948769.1">
        <property type="nucleotide sequence ID" value="XM_045092834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YV77"/>
    <dbReference type="SMR" id="A0A8I6YV77"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510530"/>
    </dbReference>
    <dbReference type="GeneID" id="123398356"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0424080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0424080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0424080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0510530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0510530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0510530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398356"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000331">
        <property type="entry name" value="Protein NRT1/ PTR FAMILY 4.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
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        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0510530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="578" mass="63037" checksum="44C5638614FBC7A3" modified="2022-05-25" version="1">MGSSFGLVDFRGRPVDTGRHGGVRASMFIYIMVWLGNVVNVANNMNMVSYLRGTMNMGVAAAATTSTNFLAALQMFTIPAAFLADSYIKRFYTVLIFAPVEILGYILLAVQAHVPSLHPPACSPSEPQSCEPVHGANLSLLLLGIYMICIGEGAIRACLPALGGDQFDNADPVERRLEASFFNWYTFSVSMGAFFGLIFIVWLENNKGWDVGFAVCAGIVLLGLLIWAAGFPFYRNQLPAGSPITRIMQVIVVAWKKRNLKLPANPDDLNQMTGDDAEGLEVLHRTEGLQFLEKAAIIDNGARGSWSLCDVTQVEETKIVCRMIPIFLTSALGYTPVSIILSFTVQQGNTMDTRLGAIRVSPATLFLIPTIFQLVILVVYDRLLVPLLRRATGYVGGVTHLQRIGVGFVATVLASAAAALVETRRKGVAEGSGLVDSPAGVPMSVFWLTPQFFLLGVVDVTSFVGLLEFFYSEASAGMKSIGSSVFYCMLGLSAWFSTLTIQLVNRVTRHGDGEAGWLDGANLNRSRLDSFYWLVCVLELVSSVVYLFLARRYLYRNDQRVAAEDDKEIPSAGYVDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YWZ0</accession>
    <name>A0A8I6YWZ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123404672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044954546.1">
        <property type="nucleotide sequence ID" value="XM_045098611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YWZ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0629140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0629140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0629140"/>
    </dbReference>
    <dbReference type="GeneID" id="123404672"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0522190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0522190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0522190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0629140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0629140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0629140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404672"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF64">
        <property type="entry name" value="OS02G0816300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="164"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="643"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
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            <begin position="464"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0629140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1212" mass="134117" checksum="316F302D0CE515B2" modified="2022-05-25" version="1">MASSTAAARLRELAPVPGAGVSAAAAAALAECCAGLLGCGDAGAACSALDALCAAGGDAMRRHADELTPLVVGRLGDGDAAVREAARRFLVLLMEMKEMNARSENTQAGSSVPDVHYSSFMTSEAESSSANQVRKNPKQKSNTRDMSLLAGEEDITRKSIEPIKVFSEKDLIKEIGKITSTLQPENDWSIRITSMQRVEGIVLGGAADYSAFPTLLKQLMPPLIAQLLDRRSSVVKQACHLLVFLSKELLRDFELCAELLIPALLKNVVITVLVIAESADNCIKEMLRNCKVARILPRIIECAKNDRSAILRGRCCEYAILMLEYWVDTPELQRSANLYEELIKCCIADATSEVRSGARACYRIFSRIWPERSHQLFSSFEPSRQKMIIDDDAETHQKQLSPIEGVKLGQPQSVPCIPTAMDKVVMVDSQTSISSGDLQSALVKIPRHSDMTLKIPAKDSKDDLATGRTFEDMSTLGKERIPVRDPDERVSGISSPSRDLPSATPFEPTQDILLSDAIVIATFQDKAECKPEVEHITSCQVQASGDPSEMLSRTPPASSEESENLLNQRPTKSSSNGISGGRLRTQQEERPCVRTPRKSVVPKHSSNNHTPNFRRPLLSKLMTNWFYASTRGDLDGKQLIIGEMANSMDVPSSLPGALSLGLNPKSDWMMRVYAFNFLRQHLLERGPKGIQEVAQNFEKVMRLVSQYLGDPHHKVAHGALSSLAEIMPVFKKPFEHYLDKMLPHVFSRLNDPKESTKQQCFAILKLAGESYSIDSLLPALLRSLDEQKSPKSKLAILEFAKASFAKCTVSSDIYSGSSFLKPWLGKLTLLFKDRNKKLKEAAVVGLSSVYCHYDPETMLSFIVTLSMEEQKQLTRAIKQLIPMIGSDLEEFLQQRRHKQKVPSFERFAATDPHPRSYVGRQHKSQQHDAYQSSDVRVNEVFSSAFQYLPSTHLEVYGRRTAKVESELGGESYGHRPETMGKIFSPARRSKDMMMNGSQSHEPSVSKMHHQDMRSVAETNSHSAPIKNLHQMSSSLLEMLDDPTESTRELALSLLAGTLEKQEKALENCIETLVVKLLHATKDSALKVVNLAHICLTTVVTQFDPLRCLLAIVSQLVSHDEKILIVSINSLSKLVMRLSHDDLMDNLSTFLPVVLDAFENHSPYVRKAILLCLVDTYLKLGPAVAPYLERLGGSQLLQLVVATSASRLSRAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZ57</accession>
    <name>A0A8I6YZ57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123410756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044959632.1">
        <property type="nucleotide sequence ID" value="XM_045103697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZ57"/>
    <dbReference type="SMR" id="A0A8I6YZ57"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648120"/>
    </dbReference>
    <dbReference type="GeneID" id="123410756"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0537910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0537910.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0537910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0648120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410756"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000012">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF607">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="98"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="238" mass="26670" checksum="D5ED6A172957F0CC" modified="2022-05-25" version="1">MSQQPEQAPVRLITAFGSPFAHRVEVALTLKGVPYELLVEDLASKSDLLLAHNPVYQSVPVLLHGDRAVCDSLVIVEYVDEAFHDDDGTAPRRLLPADPYDRATARFWADFVANKCLKPLWQSTWTDGEEQARLARETKEGLGVLEAQLDGKRFFGGEALGFVDLAACTLAHWLGVLGEVGGVRLMEDGEYPALRRWAKEYTSHEVVRRSLPDRDELVAYFTKNKEKYRSSMLKPAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZD9</accession>
    <name>A0A8I6YZD9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123406364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The PPC domain mediates interactions between AHL proteins.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955809.1">
        <property type="nucleotide sequence ID" value="XM_045099874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZD9"/>
    <dbReference type="SMR" id="A0A8I6YZD9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631450"/>
    </dbReference>
    <dbReference type="GeneID" id="123406364"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0524350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0524350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0524350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0631450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0631450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0631450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406364"/>
    <dbReference type="OrthoDB" id="688543at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039605">
        <property type="entry name" value="AHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500:SF9">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="4">
        <location>
            <begin position="301"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0631450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="555" mass="56574" checksum="68861DDA8F4D7CD2" modified="2022-05-25" version="1">MDGRESTATSGPNYSPFYVQHRGMGPPGGVTGSGLHGPPAAGYRQQQQLDAVSGGYTFHQPPFGAPAHIGQQGYHQNHVEASQQHMAQQQGAGAGAGAGGSQHMTQHSVSSGGGLQHMAQQHSAGGGGSQHMAQQHSAGGGGSQHMAQQHSAGGGGSQHMAQHGGGGGSQHMMQHNAGGGADGGMDIGMGVVAVSGDAKGDQGGDAGQDEQVKKKRGRPRKYKPDGSVTLGLSPSPSTPHSSSPGMGTMVTTPGSGFGQGTGSGGSGSGALTEKRGRGRPPGSGKMQQLASLGKWFLGSVGTGFTPHVIIISAGEDVAARIMSFSQQGPRAICIISATGAVSTATLHQDSDSGVVTYEGRFEILCLSGSYLVLEEGGTRTRSGGLCIALCGPDHRVIGGTVSGVLTAAGTVQVIVGSFMYGGGTKKNKGKADQDVENEEQNGGEDTPALALPEHPHNMPPHPMSGWPPGLMNQMDPRSSPMYGGGSNKNKTKAEQDMEDEERNSGGGGGGEEPLAMAHPEHNMNMPPPQMSGWQPGLMRQMDPRSSSIDINSIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z4P6</accession>
    <name>A0A8I6Z4P6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z4P6"/>
    <dbReference type="SMR" id="A0A8I6Z4P6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="153" mass="17435" checksum="9EF344E9076D9D2F" modified="2022-05-25" version="1">MIRDNSITQAIFNYYPQCPRPDHVLGLKAHTDGSIITVNFTDVEGLQLEKNGVWYNVPIISNALVINIGDVMEILSNGFFKSLVHRVVTNAEKERLSLVLVYTLELETQLEPVLELVDDKRHARYMKIKLNDYIEKYHDTYATGTLAIDGVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z6J1</accession>
    <name>A0A8I6Z6J1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acidic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the plant thionin (TC 1.C.44) family. 4 C-C subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z6J1"/>
    <dbReference type="SMR" id="A0A8I6Z6J1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0645060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0645060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0645060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0645060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0645060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0645060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1350.10">
        <property type="entry name" value="Thionin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001010">
        <property type="entry name" value="Thionin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036391">
        <property type="entry name" value="Thionin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920:SF3">
        <property type="entry name" value="ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920">
        <property type="entry name" value="THIONIN-2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00321">
        <property type="entry name" value="Thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00287">
        <property type="entry name" value="THIONIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57429">
        <property type="entry name" value="Crambin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00271">
        <property type="entry name" value="THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303985" description="Acidic protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0645060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14601" checksum="D2EAC3AB8F466BC2" modified="2022-05-25" version="1">METISLKSIIIGVLMLGLVLQQTHIEAKSCCCSTTTRNCYNVCRVTGSSRPTCASLCGCKILEKCVPSCNRLNLITDGDGAKSIEYCKLECMSSLCGNINAVVASEEVNDVKDHCKTGCYHLYTKDYEFGEVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZFM5</accession>
    <name>A0A8I6ZFM5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123412534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961418.1">
        <property type="nucleotide sequence ID" value="XM_045105483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZFM5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747800"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747870"/>
    </dbReference>
    <dbReference type="GeneID" id="123412534"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0620350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0620350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0620350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412533"/>
    <dbReference type="KEGG" id="hvg:123412534"/>
    <dbReference type="OrthoDB" id="672536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="18172" checksum="F36B915D90672A96" modified="2022-05-25" version="1">MGGDLFRSMTELQLIMLEMKAPELANIYVLLNKSGCSNLERLFVQFPIAPHEPLVDSFDYVGLEQPPDVLENLKVVKITNFNWNRYELQLVCILLRKASSLHKLLLITPSLEPLQVHGIQQQADLLFLAEAVANGKVILSGSDDAATQPFHSDASADFKTFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B241</accession>
    <name>A0A8I7B241_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B241"/>
    <dbReference type="SMR" id="A0A8I7B241"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0107640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0107640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0107640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0107640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0107640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF60">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
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    <feature type="domain" description="Expansin-like CBD" evidence="6">
        <location>
            <begin position="107"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="202" mass="21568" checksum="673075649CFC9384" modified="2022-05-25" version="1">MITAGGPSIFKNGKGCGACYQVTCTGNAACSGFPVTVVVTDECLGGLCLAEAAHFDLSRKAFGAMAKPGQADKLRNAGGIRVQYNRVPCNWGGVDMVFKVDASSNPSYLALLIEDEPGDGDLSAVELQQWSRGYGWEPMQELRGAVWKYNSTTALQAPISIRLTTGSGKQLVASNVIPRIWQAGRTYRYPLIALSGRVPIYG</sequence>
</entry>
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        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I7B3C0"/>
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    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0059130.1">
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF8">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23119" checksum="AEB9A2F4EDCD1215" modified="2022-05-25" version="1">MVEESKIARVNDNLESAFTIHNLKEEKNKLDANYDKLVQDVHQLMDMQEDRVVDFSYLQADHTYQQECKKAEMAKKDADKQKLNQKYEILCNLTSAQATVIQNFKFKNMKEKELLSEAKMNLELKNAEFTKFEEKLSQEKLELKFQAADLLKGKEKHNEEKYMLKLKIVELMKAEEDLKEKIKGIQAILHNRTRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3C8</accession>
    <name>A0A8I7B3C8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3C8"/>
    <dbReference type="SMR" id="A0A8I7B3C8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059970"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059970"/>
    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="7"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="8">
        <location>
            <begin position="128"/>
            <end position="222"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="24687" checksum="BC1FF06531DB9852" modified="2022-05-25" version="1">MLGSGPFNIRSGTSMSTPHVSGITALVKSSHPDWSAAAIKSAILTTTDITDSAGGPILDEQHQKATAYAMGAGHANPIKAIDPGLVYDLSITEYAGYICALLGDQGLASIARNPMLSCKMLPKIPEAQLNYPSITVPLRTRPFTVHRTVTNVGPPNSVYTLKMVVPKSLRVCVYPETLVFSKAGEKLTYSITASTNSTGGKKFMEGTLSWISKNHVVRSPILFVVGLDSPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4I6</accession>
    <name>A0A8I7B4I6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4I6"/>
    <dbReference type="SMR" id="A0A8I7B4I6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0221500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="14522" checksum="C02D0C83636C324D" modified="2022-05-25" version="1">MYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYCVEQRAILDEMKRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEAGDGKGKYPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5E8</accession>
    <name>A0A8I7B5E8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123443882</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976359.1">
        <property type="nucleotide sequence ID" value="XM_045120424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5E8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0271710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0271710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0271710"/>
    </dbReference>
    <dbReference type="GeneID" id="123443882"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0225190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0225190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0225190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0271710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0271710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0271710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443882"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000033">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050974">
        <property type="entry name" value="Plant_ZF_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12506">
        <property type="entry name" value="PROTEIN PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12506:SF50">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="392"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="447"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="392"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="447"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0271710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="500" mass="54722" checksum="716F8509B9CF0CC1" modified="2022-05-25" version="1">MSGYPFYPYGRGGAGDDAAAMSGYPFYPYGRGGVGDDAAARYSSYEIDLIAARYAGDPSPYPYPYPSASGEFDTHVGARRPADVLYHRPIMGSHSTIGQSDDFYSPNTMAKRPRLESSLPIYPQRPGEKECAFYMRTRTCKYEETCKFDHPQWVPKGGIPKWKEGQKAEESYPERPGKPDCPFFVKTGMCKFGSECKFNHPKKKDDGTVAGSGDKESLLSGNSTLPVKPSEPCPVSLISGNSTLPVKPFKPCPHYAKGKCKLGTNCKFSHAKDMEAPSSGENESENTATAEAAGHNGAVHDLVSAKKVAAVALEQNSKGMPIRPGEVDCSFYIKTGSCRYGRACRFNHPERHNEASVVPGKILNPAAKFLPSFDFRAAHVPIEPEPITYPQRPGETVCDFYKKTGFCKFSERCKFHHPVDRSAPGSIAKRESSQQPVMLTVAGFPRREDAEACAYYMKTGACKFGVQCKFDHPPLGEVIANVSKQGDGKKVVGLSYVLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6P3</accession>
    <name>A0A8I7B6P3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldose 1-epimerase</fullName>
            <ecNumber evidence="5">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123426900</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aldose epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044966741.1">
        <property type="nucleotide sequence ID" value="XM_045110806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6P3"/>
    <dbReference type="SMR" id="A0A8I7B6P3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173090"/>
    </dbReference>
    <dbReference type="GeneID" id="123426900"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0143050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0143050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0143050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0173090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426900"/>
    <dbReference type="OrthoDB" id="274691at2759"/>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000008">
        <property type="entry name" value="Aldose 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035245854" description="Aldose 1-epimerase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="96"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
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        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="39646" checksum="4ABF84E7DFB16E32" modified="2022-05-25" version="1">MARAPLLLLLPLLLAALCLASLAPGAIAAGRKTVGVYELRKGDFSAKVTNWGATVTSVVFPDSKGNLGDVVLGYDTIAEYVNGSSYFGALVGRVANRVAKARFVLDGKVYHLYANDGKNALHGGHRGFSKVIWTVKEYVSGGDSPHITLYYHSFDGEQGFPGALDVYVTYELSSPYVLSVRMNATALDKATPVNLAQHTYWNLGGHGSGDVLGNTVQLLASRYTPVDATLIPTGQLAPVAGTPYDLRAPTAVGEHLRQVVGGSSGGSAVYGYDINYVVDGDARALRKVAAVRDGASGRALELWADQPGVQFYTGNFLKDVKGKGGETYGQYAALCLETQGFPDAVNHPEFPSQIVRPGEAAYKHDMVFKFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B839</accession>
    <name>A0A8I7B839_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A8I7B839"/>
    <dbReference type="SMR" id="A0A8I7B839"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="156"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0429430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="483" mass="56776" checksum="86E8B61AD753EEF3" modified="2022-05-25" version="1">MEKYKVAKKAAKRAVSEARGRAYEDLYQRLNTKEGERDIYKMAKIRERKTRDVGEVKCIKDGDDQLLVKDEAIKRRWREYFDNLYNGEVDSSTIELDDSFDDTSMCFVRRIQECEVKEALKRMKVGKAMGPDGIPIEVWRGLGDIAIVWLTKLFNLIFRSNKMPEEWRRSILVPIFKNKGDVQSCTNYRGIKLMSHTMKLWERVIEHRLRRLTSVTKNQFGFMPGRSTMEAIFLVRQLMERYKEQKKDLHMVFIDLEKAYDKIPRNVMWWALEKHKVPIKYITLIKDMYDNVVTSVRTSDGDTDDFPIRIGLHQGSALSPYLFDLVMDEVTRDIQGDIPWCMLFADDVVLVDDSRTGVNRKLELWRRTLESKGFRLSRTKTEYMRCNFSATRHEDGEVSLGGQVVPERDTFRYLGSMLQKDGDIDEDVGHRIKAGWMKWRQASGVLCDKRVPQKLKGRFYRTAIRPAMLYGAECWPTKRRHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B957</accession>
    <name>A0A8I7B957_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123439433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035231322" evidence="1">
        <location>
            <begin position="27"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="18637" checksum="DF9C7A8952B71287" modified="2022-05-25" version="1">MATSFSCFLDISILVVFFSLPSLTGARLLYADKNTLNDSKSFSIGGGRGKGGGRGFGVSIRHGGHDTSIAIGGGLGGGAATNHGGGTSVGGGAGAGIGIDIGHGGVDVGIGGGGGGAASTGGVHAGGGGGGGIGVHIGHRGVTISAGGGGGAGVGAGGSGGGEGGGSGVGRAGNAVGGGGGYGSANGGNGSGGGTGVGSAGGNAGGGSGNGGVIHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAT6</accession>
    <name>A0A8I7BAT6_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC123402902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044952803.1">
        <property type="nucleotide sequence ID" value="XM_045096868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAT6"/>
    <dbReference type="SMR" id="A0A8I7BAT6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540930"/>
    </dbReference>
    <dbReference type="GeneID" id="123402902"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0449870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0449870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0449870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402902"/>
    <dbReference type="OMA" id="VGMFTIK"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009807">
        <property type="term" value="P:lignan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF57">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="8"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="313" mass="34044" checksum="FE37A651A9F3C5D5" modified="2022-05-25" version="1">MALNKGRSRVLVIGGTGHIGKHIVAASARHGHSTSVLIRDVAPSDLAKMQLLKSFIDTGVALIKGDLFDHGSLVNAIKGADVVVSAVGPRLVAEQTRIVMAIKESGNVKRFLPSEFGSDVTQVHTVDPAAALFARKVSIRRLIEAEGIPHTYVCCNCFAETYLPSIGDVTAVGAGPPSDKITVLGDGDAKAVFVVEEDIAAYTMRAVDDPRTLNKILYMRPRANILSHNELISMWERKVGRRFQIVRIPEADLLKLIKESAFPLNILLSLALSIFVGGDQANFKIEPSFGVEATELYPDLKYNTVDEYLDRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BBP0</accession>
    <name>A0A8I7BBP0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BBP0"/>
    <dbReference type="SMR" id="A0A8I7BBP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0342820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0342820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0342820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0342820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0342820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0342820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0342820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25801" checksum="914A3D9103D96030" modified="2022-05-25" version="1">MSVCGYRWYPEYTMEEQYRDFNQSQYICTVRVFPDYPGAKEPIHWSYGLGVSVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLGGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSLARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BC19</accession>
    <name>A0A8I7BC19_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc finger-XS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC19"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0360880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0360880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0360880"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005381">
        <property type="entry name" value="Znf-XS_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03470">
        <property type="entry name" value="zf-XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger-XS" evidence="1">
        <location>
            <begin position="64"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0360880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="112" mass="13118" checksum="33BFEA989EC481A7" modified="2022-05-25" version="1">MKRKLAKTWFKSPYDSDYDDDEPYEYNSRDDVEDGNKESFIEKEAQTIKEKWKAAYFMRGMFRCPYCTTKPKPRDGVYEHLMSHARGLSQSADQDIKTRAEHAALLKTLGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BC78</accession>
    <name>A0A8I7BC78_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123403843</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953700.1">
        <property type="nucleotide sequence ID" value="XM_045097765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC78"/>
    <dbReference type="SMR" id="A0A8I7BC78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0604140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0604140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0604140"/>
    </dbReference>
    <dbReference type="GeneID" id="123403843"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0500770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0500770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0500770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0604140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0604140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0604140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403843"/>
    <dbReference type="OMA" id="TMVYEED"/>
    <dbReference type="OrthoDB" id="664987at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027214">
        <property type="entry name" value="Cystatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47116">
        <property type="entry name" value="PHLOEM FILAMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035212959" description="Cystatin domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="34"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0604140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="119" mass="12652" checksum="59259412F34B4374" modified="2022-05-25" version="1">MRTTSFLLLLLAGAANLYLIVSPTKALGGEWQPIPNVADAHVQGIGKCAVDEQNKVTNCGLRFAKVVSGKVQNTGVTTYLLDVDALRLDGSHKIYQVEVVEQNSSGSSTCKLVSFGPNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCQ2</accession>
    <name>A0A8I7BCQ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">laccase</fullName>
            <ecNumber evidence="6">1.10.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCQ2"/>
    <dbReference type="SMR" id="A0A8I7BCQ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0520940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0520940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0520940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF242">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0052">Apoplast</keyword>
    <keyword evidence="12" id="KW-0186">Copper</keyword>
    <keyword evidence="13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="62"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="360"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="500" mass="55107" checksum="F854366FAF9DD8DF" modified="2022-05-25" version="1">MITQCPIQPNTTFTYRFEVSGQEGTLWWHSHVSALRATLHGIILIRPRSGSSYPFPKPDVEVPVIIGEWWQRDLVKVDNNFSIGGSFDDNPAAAAINGKLGDLYNCSGVAEDNFVLDVEPGKTYMLRLVNAALFSEYYFKVAGHKLTVVGADANYARPFTTDVIAVAAGETIDVLMLADAPPCRYYMAALANQPPVPDPQIPVFVTRGVVQYKNISSSDAQNCSRDEPPLMPAMPDQHDTITTFYFHGNLTGLQPGSNPLLPQVRERVDERLFLTLGKGSMCTHNKTSCKRGGSSESFEVAYINNVSFHLPETTAVLQARYHRGGKLNGMNGGVPVQELPSRPPRAFNFTDTALIPVVPGGRMEELEPTRKATMTRRFAYNATVEVVFQSTATMQSDSNPMHLHGHDFFVLAQGHGNHDAARDVRSYNLVDPPMKNTVQVPRLGWAAIRFVADNPGAWFLHCHFEFHMAMGMAAVFEVDNGPTLETTLPPPPSDLPKCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCY8</accession>
    <name>A0A8I7BCY8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123449464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982636.1">
        <property type="nucleotide sequence ID" value="XM_045126701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCY8"/>
    <dbReference type="SMR" id="A0A8I7BCY8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400760"/>
    </dbReference>
    <dbReference type="GeneID" id="123449464"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0333480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0333480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0333480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0400760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449464"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010009">
        <property type="term" value="C:cytoplasmic side of endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000851">
        <property type="entry name" value="Ras-related small GTP-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="21473" checksum="FCEE5869F2E408FA" modified="2022-05-25" version="1">MGCSSSAPARSTGGLNNINGSSSATDSNDLRAKLVLLGDSGVGKSCIVLRFVRGQFDPTSKVTVGASFLSQTLALEDSTIVKFEIWDTAGQERYAALAPLYYRGAAAAVVVYDITSPESFKKAQYWVKELQKHGNPGIVMVLVGNKADLHESRSVPSQEAQEYAEKNSMLFMETSAKTSDNINQVFEEIAKRLPKPTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDU5</accession>
    <name>A0A8I7BDU5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDU5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0364870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0364870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0364870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0440760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="193" mass="21011" checksum="F014339C401F097D" modified="2022-05-25" version="1">MPNKTVLATSLDKMTNHGDVFKMKLLMYLISSVFAPTTSLRSSNKCFPVSLMYVDHLDLSTVDIDVLGGPPSPHKFVFSMWAYSFVGEHTHRPTGCRGRYDYNSSHELPKTQEELFGDGYGGMDAGPAKDDHDDTENVQHGSGRMKSREAPAGECNRKAPDVVPPLKGGNVSTFASDAVVSPSKRGRTTKDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BE25</accession>
    <name>A0A8I7BE25_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123452372</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044984956.1">
        <property type="nucleotide sequence ID" value="XM_045129021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BE25"/>
    <dbReference type="SMR" id="A0A8I7BE25"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0455740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0455740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0455740"/>
    </dbReference>
    <dbReference type="GeneID" id="123452372"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0377220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0377220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0377220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0455740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0455740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0455740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452372"/>
    <dbReference type="OMA" id="KKTHDMY"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="141"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="331"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0455740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="530" mass="57963" checksum="F8B2CDD7D92C7242" modified="2022-05-25" version="1">MDPELLSMQEELSNDRKKDTKSKKAKKPKPAAEEAAAEEDDLSNKRKKDKKSKKDKKRKLAAENEEAATEEEGKSSKKRGVTKEEPDQVGAVEKSVAVTGKGFADPKYAPLKSFAAAALPTQVLDCCKGFDRPSPIQALAWPYLLDGRDFIGIAATGSGKTIAFGVPALMHVRKKLSEKGAKKGLPRCLMLAPTRELAQQIADVLTEAGAPCGINSVCLYGGTSKGPQISSLKSGVEIVIGTPGRMKDLIEMGVCRLNEVSFVVLDEADRMLDMGFEPEVRAILSQTSSVRQMVMFSATWPFAVHQLAQEFMDPNPIKVVVGSEDLAANHDVMQIVEVLDDRARDSRLVALLDKYHRAQSNRVLVFVLYKKEAGRVEAMLNKRGWKAVSVHGDKAQHDRTKALSLFKEGKCPLMIATDVASRGLDIPDVEVVINYSFPLTTEDYVHRIGRTGRAGKKGVAHTFFTQADKGLAGELVNVLREADQVVPPALTKFGTHVKKKESKIYGSHFKEITADAPKSTKITFGDSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFQ6</accession>
    <name>A0A8I7BFQ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123407203</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956219.1">
        <property type="nucleotide sequence ID" value="XM_045100284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFQ6"/>
    <dbReference type="SMR" id="A0A8I7BFQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747760"/>
    </dbReference>
    <dbReference type="GeneID" id="123407203"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0620310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0620310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0620310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407203"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020">
        <property type="entry name" value="OSJNBB0059K02.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020:SF33">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288344" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40671" checksum="8D3EAD8E26A908E8" modified="2022-05-25" version="1">MKLPPAVVWLLLVVLFFDAARVEARGTPSAEESKNQWTSMFVFGDDFADNGNLPKLPGGHSQSDLSRQWIYPYGSYINSRGSAAAVPTGRFSNNKIQSDFIARILGLNAAPPAYMHTLDQSCDPTGMTFASGGAGVFQKKVPTLAAQVKSFMRLIKSGIISKEQLRHSVALVAISGNDYMSGADVKNSFLSSFDDIDTYIGNVTTEIVKNVEQLQKMGVKKVLVNNMLPIGCTPLRTSPNNYTACDILGNYAASVHNKNLKQLMDKKNNAYMLDLYTAFTDIINHAPVEGSDQSNNKFNNNLAPCCEGFYDTGFCGQQGDTGEPLYELCENPDQLFYWDEVHPTHAGWKAVMKALEQPLKEFLDRDYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-09-23" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>B5B0D1</accession>
    <name>B5B0D1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NBS-LRR resistance protein analog 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">RGA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RGA1</name>
        <name evidence="14" type="synonym">LOC123453035</name>
        <name evidence="13" type="synonym">Rga1</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2008" name="Proc. Natl. Acad. Sci. U.S.A." volume="105" first="14970" last="14975">
            <title>The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains.</title>
            <authorList>
                <person name="Brueggeman R."/>
                <person name="Druka A."/>
                <person name="Nirmala J."/>
                <person name="Cavileer T."/>
                <person name="Drader T."/>
                <person name="Rostoks N."/>
                <person name="Mirlohi A."/>
                <person name="Bennypaul H."/>
                <person name="Gill U."/>
                <person name="Kudrna D."/>
                <person name="Whitelaw C."/>
                <person name="Kilian A."/>
                <person name="Han F."/>
                <person name="Sun Y."/>
                <person name="Gill K."/>
                <person name="Steffenson B."/>
                <person name="Kleinhofs A."/>
            </authorList>
            <dbReference type="PubMed" id="18812501"/>
            <dbReference type="DOI" id="10.1073/pnas.0807270105"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2008-07" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Brueggeman R.S."/>
                <person name="Druka A."/>
                <person name="Nirmala J."/>
                <person name="Cavileer T."/>
                <person name="Drader T."/>
                <person name="Rostoks N."/>
                <person name="Mirlohi A."/>
                <person name="Bennypaul H."/>
                <person name="Gill U."/>
                <person name="Kudrna D."/>
                <person name="Whitelaw C."/>
                <person name="Killian A."/>
                <person name="Han F."/>
                <person name="Sun Y."/>
                <person name="Gill K."/>
                <person name="Steffenson B."/>
                <person name="Kleinhofs A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="journal article" date="2013" name="Phytopathology" volume="103" first="1153" last="1161">
            <title>Allele characterization of genes required for rpg4-mediated wheat stem rust resistance identifies Rpg5 as the R gene.</title>
            <authorList>
                <person name="Arora D."/>
                <person name="Gross T."/>
                <person name="Brueggeman R."/>
            </authorList>
            <dbReference type="PubMed" id="23841622"/>
            <dbReference type="DOI" id="10.1094/PHYTO-01-13-0030-R"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="5">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="6">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="EU883583">
        <property type="protein sequence ID" value="ACG49265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU812563">
        <property type="protein sequence ID" value="ACJ10039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KC433594">
        <property type="protein sequence ID" value="AGO59120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5B0D1"/>
    <dbReference type="SMR" id="B5B0D1"/>
    <dbReference type="STRING" id="112509.B5B0D1"/>
    <dbReference type="PaxDb" id="4513-MLOC_6270.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0526160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0526160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0526160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0437530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0526160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0526160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0526160"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_25_2_1"/>
    <dbReference type="OMA" id="SHIRMAK"/>
    <dbReference type="OrthoDB" id="629866at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5B0D1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1227">
        <property type="entry name" value="OS11G0462500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="137"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="357"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="501"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACG49265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ10039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGO59120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0526160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="895" mass="99789" checksum="8D046485F9BC950B" modified="2008-09-23" version="1">MEVAVVVGPIVKLLPKLLSVIDGKRKQLDSLEVDAGFIRRDLQSIQEIIGRSNGSRSITDLWVRDLRRLADDMEDCIDRFQVGKMSRISFVGKICKLKKRSKETLEQLQNCISIAGAAAPAPDPPSAPGGTDQDPEEQLLGLLARSQPEGNLKVISVAGFSGVGMTRLAHKVYSDRDVRSQFPLHAWVRAAPGMSVLKLLQKIHDQLMLISITKDHGETASNSKIIPQINGNAQHAVSDHPLAGLLKTGRYLIVIDGVNTHELYDVLSAFSWADGMDGRIIMTTTIQLPAATCCKCGNGSSLAMDSTSQVFIGELTESGFVESCLHLRDDTLARMRRSSKEILSSPVVQDLLLYFCMFPRDHPVRRNPLIRRWLAEGLVFPQPETEIFSQDVAVKNLESLISRNIIQPIQVRTYGNVKKCQTSGIMLNSISSKSKSQNFITMLCGGQTTEKNLPGKEIRRLSLHLNGAANGPLDLPKQLSRLHTLAVFPDDTNITRHQANLNYAEYKLLRVLDLKECADVKAEHVGKICDLLLLKYLSLGDSIDKVPRKIVKLKWLETLDMRRTQVVMLPIEVLHLPGLKHLLGKLQLLEGDCTQKKLEKLLSEDSELQRLSGFVTDKSEGFAQLMGRMVKLRKVKIWCDSSADVTKLDHVLGAMKKFIREGLDMTRVDRSLSMDFQGCPTECSERFMDSLQATGRLTSLKLRGKLTQLPQFQAKLNYIEELCLSRTNLSGDTILDGLSELRMTLKYLKLVEDKLEHLVIKPEHFRSLKGLCLVGEQRMQDITIQDEAMPYLVSLHILCEALGNLPGVDITHMKRLKEVALHSGVEEMIKDGWQTAAMNHPNRPNVLFIHHANSSPRDSPPCQATVEIANRTKPIGRKFTASIMGGIFACARPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQ95</accession>
    <name>F2CQ95_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123444455</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AK353797">
        <property type="protein sequence ID" value="BAJ85016.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977100.1">
        <property type="nucleotide sequence ID" value="XM_045121165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQ95"/>
    <dbReference type="SMR" id="F2CQ95"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0290180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0290180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0290180"/>
    </dbReference>
    <dbReference type="GeneID" id="123444455"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0241030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0241030.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0241030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0290180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0290180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0290180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444455"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14663">
        <property type="entry name" value="STKc_SnRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000653">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000015">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF140">
        <property type="entry name" value="CBL-INTERACTING SERINE_THREONINE-PROTEIN KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="49"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="17">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="334"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ85016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0290180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="519" mass="57719" checksum="9BC716FAE29F5132" modified="2011-05-31" version="1">MAAVKPPPPDPSPQAARLPSPSSSSSSASAAAAAKRGGTGSRGLLMGRYELGRVLGKGTFAKVYHARHLQTGESVAIKVLDREKAVRSGLVSHIKREIAVLRRVRHPNIVHLFEVMATKTKIYFVMELVRGGELFSRVSKGRLKEDTARRYFQHLISAVGFCHTRGVFHRDLKPENLLVDEAGNLKVSDFGLSAVAEPFQPEGLLHTFCGTPAYVAPEVLARRGYEGAKADIWSCGVILFVLMAGYLPFHDQNLMAMYRKVYKGEFRCPRWFSKDLTSLIMRFLDTNPSTRITLPEVMESRWFKKGFRPVKFYIEDDQLYNVIDAENDMLDLGLSDPLPQPLPPPPPPPQEVDGNDSGSESDSSVMSCPATSSFEERQRLRGPLPRPASLNAFDIISFSRGFNLSGLFEEKGDEVRFISSEPMSDIITKLEEIANVKSFAVRKKDWRVSLEGTREGVKGPLTIGAEIFELTPSLVVVEVKKKAGDKEEYDDFCNKELKPGMEHLVHQMVPVPNTPTISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSS2</accession>
    <name>F2CSS2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK354674">
        <property type="protein sequence ID" value="BAJ85893.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CSS2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0132830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0132830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0132830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0160950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160950"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CSS2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF10">
        <property type="entry name" value="OS04G0376100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ85893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="20758" checksum="EF8A6D5306A9AE3A" modified="2011-05-31" version="1">MSTPAGDGEATGAGTPEAAAPFSADWKERILVPVVAAGVVGAGFGLLSRHRARLGPIRAAATYAANLSIVAGCYGGARELARDARATTPDDLMNSVVGGLASGAVLGRIQGGHFGAVKYAVTLAVAGTALDYFAMKLRPQWLDWKEHLSVDTKDWFTVPEWSPIQVLDEEALAKKREREKLLFAQRALGKVSKEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0C1</accession>
    <name>F2D0C1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123440048</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AK357328">
        <property type="protein sequence ID" value="BAJ88542.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972579.1">
        <property type="nucleotide sequence ID" value="XM_045116644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0C1"/>
    <dbReference type="PaxDb" id="4513-MLOC_70309.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059350"/>
    </dbReference>
    <dbReference type="GeneID" id="123440048"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0059350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0059350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0059350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440048"/>
    <dbReference type="HOGENOM" id="CLU_010485_2_3_1"/>
    <dbReference type="OMA" id="LWEVESH"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D0C1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000170">
        <property type="entry name" value="Probable methyltransferase PMT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF37">
        <property type="entry name" value="METHYLTRANSFERASE PMT6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ88542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0059350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="600" mass="68058" checksum="02701CA17F168D11" modified="2011-05-31" version="1">MGRWCSPASAEPRSVQLLLLGVALVAASFYAGTLFQSSASPALILPPSASRSSGSSEPQGASELTNKVALTYRTALISVPAHGLDVCPLEHNEYVPCHDAAYVSKLRELDRSRHENLEAKCPPREESLFCLVPPPNDYKIPIRWPTSRDYVWRSNVNHSHLSEVKGGQNWVHENGKLWWFPGGGTHFKHGATEYIERLGNMTTNSTGDLRSAGVVQVLDVGCGVASFSAYLLPLDIHTMSFAPKDGHENQIQFALERGIGAMISVLATKQLPYPGNSFEMVHCSRCRVDWHENDGILLKEVDRLLRPNGYFVYSAPPAYRKDKDFPIIWEKLINITTSMCWKLIAKHVQTAIWIKPEDESCRQKNADMGILNICDPSDTSSWQAPLMNCVRLNTDQLKIQKLPSRPERLLFYSRSLELIGVTPEKFENNNQFWRDQVRKYWSFLGVEKTSIRNIMDMNANYGGFAMALSTDPVWIMNIVPNTTINTLPVIYDRGLIGSYHDWCQPFSTYPRSYDLLHAFHLFSHYQGHAGGCLLEDIMLEIDRIIRPQGFIIIRDENTTLSRISDLAPKFLWDVTTRTLENEENRPEQVLICRKKFWAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0F5</accession>
    <name>F2D0F5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123444131</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357362">
        <property type="protein sequence ID" value="BAJ88576.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976684.1">
        <property type="nucleotide sequence ID" value="XM_045120749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0F5"/>
    <dbReference type="SMR" id="F2D0F5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280830"/>
    </dbReference>
    <dbReference type="GeneID" id="123444131"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0232770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0232770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0232770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0280830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444131"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031567">
        <property type="term" value="P:mitotic cell size control checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010091">
        <property type="term" value="P:trichome branching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF24">
        <property type="entry name" value="HISTONE CHAPERONE ASF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ88576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="185" mass="20853" checksum="DC514D93CAECA59E" modified="2011-05-31" version="1">MSAVNLTNVAVLNNPTSFVNPFQFEISYECLVALEDDLEWKLIYVGSAEDENYDQQLESVLVGPVNVGTYRFVLQADPPDPSKIREEDIIGVTVLLLTCSYMGQEFIRVGYYVNNDYDDEQLREEPPAKLLIDRVQRNILTDKPRVTKFPINFHPETSGGQQQDQPQSAVPENHTGEGSKANTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2D4B9</accession>
    <name>F2D4B9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123408875</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK358728">
        <property type="protein sequence ID" value="BAJ89940.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957845.1">
        <property type="nucleotide sequence ID" value="XM_045101910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D4B9"/>
    <dbReference type="SMR" id="F2D4B9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708870"/>
    </dbReference>
    <dbReference type="GeneID" id="123408875"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408875"/>
    <dbReference type="HOGENOM" id="CLU_002706_37_6_1"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000381">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000285">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF470">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="127"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="329"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="467"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="545"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ89940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="637" mass="70090" checksum="FA5AAD413C21792E" modified="2011-05-31" version="1">MLPSILSKSRKAPSPAGHCRRLRRLLSSSLGTARALPPNTGFAASPPPPSTNPRLASSPHAYHHHTSILQSCVASGSLRAGRQLHGRLLVSGLGPDTVLSTKLVDLYAACGQVGHARRLFDGMPKRNVFLWNVLIRAYAREGPREAAVRLYRGMVEHGVEPDNFTYPLVLKACAALLDLETGREVHQRVSGTRWGQDVFVCAGVVDMYAKCGCVDDARAVFDGIAVRDAVVWNSMIAAYGQNGRPMEALALCRDMAANGIGPTIATLVSAVSAAADAAALPRGRELHGFGWRRGFGLQDKLKTSLVDMYAKSGWVQVARVLFEQLMKRELVSWNAMICGYGMHGHADEALALFNKMKGDAQVTPDNITFVGVLSACNHGGMVEEAKEFFYLMVNAYSIKPTVQHYTCVIDVLGHTGRFEEAYDLIKGMSIEPDSGIWGALLNGCKIHKNVELGELALQKLIELEPEDAGNYVHLSNIYAQSGKWEKAARVRKLMTNRGLKKILACSWIELKGKTHGFLVGDASHPRSDEIYGELERLEGLMSDAGYVPDIIPVFHNVDDDEKRNMVRSHSERLAIAFGLISTPPGTKLLVTKNLRVCEDCHVVIKLISQIVQREIIIRDVNRYHHFVNGECSCKDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5A1</accession>
    <name>F2D5A1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123447016</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
            <tissue evidence="18">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant 'ANKYRIN-BTB/POZ' family. 'NPR1-like' subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK359061">
        <property type="protein sequence ID" value="BAJ90272.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366647">
        <property type="protein sequence ID" value="BAJ97850.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979505.1">
        <property type="nucleotide sequence ID" value="XM_045123570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5A1"/>
    <dbReference type="SMR" id="F2D5A1"/>
    <dbReference type="STRING" id="112509.F2D5A1"/>
    <dbReference type="PaxDb" id="4513-MLOC_51988.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333690"/>
    </dbReference>
    <dbReference type="GeneID" id="123447016"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333690"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034895_1_0_1"/>
    <dbReference type="OrthoDB" id="71307at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D5A1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000031">
        <property type="term" value="P:regulation of salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009862">
        <property type="term" value="P:systemic acquired resistance, salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18310">
        <property type="entry name" value="BTB_POZ_NPR_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000123">
        <property type="entry name" value="regulatory protein NPR3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044292">
        <property type="entry name" value="NPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021094">
        <property type="entry name" value="NPR1/NIM1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024228">
        <property type="entry name" value="NPR_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057250">
        <property type="entry name" value="Znf_C2HC_NPR-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46475:SF4">
        <property type="entry name" value="BTB_POZ DOMAIN AND ANKYRIN REPEAT-CONTAINING PROTEIN NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46475">
        <property type="entry name" value="REGULATORY PROTEIN NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11900">
        <property type="entry name" value="DUF3420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12313">
        <property type="entry name" value="NPR1_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52046">
        <property type="entry name" value="ZF_C2HC_NPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="56"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC NPR-type" evidence="16">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="563"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ90272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ97850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="589" mass="65501" checksum="A5A8FBA3CF9A801A" modified="2011-05-31" version="1">METSTVTYSSSSPPPSSSPQQQQAEQAADLDAVSLGRLSANLERLLDPAFLGCADADVVLAAGDAVVGVHRCILAARSNFFLDHFSSLAADDKERPRLELADLVPGGRHIGRDALVAVLGYLYTGRLRPPPRDAAVCVDELCPHEACRPAIDFVVESTYAAAGFQISELVSLFQRRLSDFVNIALVEDIVAIVHVASTCQLHELLSQCIQRVAGSSVDSRYLEKELPDEAFAKVKEFRRYSLHDDSDEFILDPEHARKVRNIHKALDCDDVDLVGLLLQESGITLDDAFAIHYAAAYCEPKVLAGILKLVPAGDCVNVNLKNDSGYTPLHLACMRREPQILLSLIEKGASVVERTRDGRDALTICKRLTTEKDCNKKFEKCKERSKAYLCIDILEQEIKRKSFIFEELLSAEVSVATPLLVDNFHMRLINLENRVAFARIFFPSEAKLVMRIAQADSTEEFAGLTSANFNKLKEVDLNETPTMQNKRLRERLDALTKTVELGRKYFPHCSDVLDKFLIEESTDLHFLESGTAEDQCTRRSRFSELKEDVRKAFSKDKAVAAIASSTSSYSPRGDGKVNRHGHKKARLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5C3</accession>
    <name>F2D5C3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123427334</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AK359083">
        <property type="protein sequence ID" value="BAJ90294.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK365921">
        <property type="protein sequence ID" value="BAJ97124.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967285.1">
        <property type="nucleotide sequence ID" value="XM_045111350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5C3"/>
    <dbReference type="SMR" id="F2D5C3"/>
    <dbReference type="FunCoup" id="F2D5C3">
        <property type="interactions" value="2060"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2D5C3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183920"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183920.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183920.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183920"/>
    </dbReference>
    <dbReference type="GeneID" id="123427334"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0152210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0152210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0152210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0183920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0183920.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183920.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427334"/>
    <dbReference type="eggNOG" id="KOG2334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="GPIRTNS"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D5C3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002943">
        <property type="term" value="P:tRNA dihydrouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ90294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ97124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="320" mass="35751" checksum="324913829E45807D" modified="2011-05-31" version="1">MDYRNKLVLAPMVRAGTLPFRLLAAEYGADITYGEEIIDHKFVHCQRVTNESLGTTDFLERGTDSVVFRTCPQERDRVVFQMGTSDAVRALKAAEIVCNDVAAIDINMGCPKAFSVSGGMGSALLSKPELIHDILTTLRRNLNTPVTCKIRLLNTRQDTVELARRIEKCGVPALAVHGRKVKDRPRDPAKWDEIADVVSALSIPVIANGDVFEYEDFKRIKDATGATSVMAARGALWNASIFSPNGKVPWEDFKREYVRKTILWDNCIKSTKTTLREIIMHYICLELPEGKGVIKCGSSADVARLYGEEDYYNFVVSNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F2D6I8</accession>
    <name>F2D6I8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="12">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123406314</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="12"/>
    <dbReference type="EMBL" id="AK359500">
        <property type="protein sequence ID" value="BAJ90709.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955733.1">
        <property type="nucleotide sequence ID" value="XM_045099798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D6I8"/>
    <dbReference type="SMR" id="F2D6I8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592050"/>
    </dbReference>
    <dbReference type="GeneID" id="123406314"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0490690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0490690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0490690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592050"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_3_1"/>
    <dbReference type="OMA" id="FMAHMAC"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D6I8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF112">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GAPC1, CYTOSOLIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 9" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="13">
        <location>
            <begin position="4"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="13"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="213"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="10">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ90709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="337" mass="36612" checksum="137A47894C90DF8E" modified="2011-05-31" version="1">MAPIKIGINGFGRIGRLVARVALQCPDVELVAVNDPFITTDYMTYMFKYDTVHGQWKHHEVKVKDSKTLLFGEKEVAVFGCRNPEEIPWAAAGAEYVVESTGVFTDKDKAAAHIKGGAKKVIISAPSKDAPMFVCGVNEKEYKSDIDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHAMTATQKTVDGPSSKDWRGGRAASFNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTVDVSVVDLTVRLAKPATYEQIKAAIKEESEGNLKGILGYVDEDLVSTDFQGDSRSSIFDAKAGIALNDNFVKLVSWYDNEWGYSTRVVDLIRHMHSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2D710</accession>
    <name>F2D710_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447066</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The RING-type zinc finger domain is responsible for E3 ligase activity.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="AK359672">
        <property type="protein sequence ID" value="BAJ90881.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979565.1">
        <property type="nucleotide sequence ID" value="XM_045123630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D710"/>
    <dbReference type="SMR" id="F2D710"/>
    <dbReference type="PaxDb" id="4513-MLOC_66488.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334180"/>
    </dbReference>
    <dbReference type="GeneID" id="123447066"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278640.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447066"/>
    <dbReference type="HOGENOM" id="CLU_055198_0_0_1"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D710">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044390">
        <property type="term" value="F:ubiquitin-like protein conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16745">
        <property type="entry name" value="RING-HC_AtRMA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000523">
        <property type="entry name" value="RING finger protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ90881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="238" mass="24942" checksum="96CA2757BC47523F" modified="2011-05-31" version="1">MAANVGESAAVGGSSSSDAAGGSFECNICFELPQEPIVTLCGHLFCWPCLYRWLHMHANTPECPVCKAIVEEDKLVPLYGRGKDRVDPRSKNTPGADIPQRPAGQRPATAPQADPNNNFGNAHANPWFMGVGGAGAGAGVPLANARWGNYAFSAAFGGLFPMLSFQMHGFPDPAAYAQPAGFHYGFGHGHGFHGGHMGPAHGVPRQGPLGQQQQQADVYLKALLIMVGVLVIASLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7U8</accession>
    <name>F2D7U8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123395639</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AK359960">
        <property type="protein sequence ID" value="BAJ91169.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK364584">
        <property type="protein sequence ID" value="BAJ95787.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946600.1">
        <property type="nucleotide sequence ID" value="XM_045090665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D7U8"/>
    <dbReference type="SMR" id="F2D7U8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0443080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0443080"/>
    </dbReference>
    <dbReference type="GeneID" id="123395639"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0366920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0366920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0366920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0443080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0443080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0443080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395639"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prefoldin subunit 3"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ91169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ95787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="195" mass="21617" checksum="628D0B238BC70AA2" modified="2011-05-31" version="1">MAAATSSSAVAVSTPQGVAERRGIPAASFVEDVETYLRQAGLEVNSALAFLQERLQQYKMVEMKLLAQQRELQAKIPDIEKCLDIVATLKAKKALGEALISDFELSEGIYSRAKIEDSDSVCLWLGANVMLEYSCDEANELLKSNLDNARASLEVLVGDLHFLRDQQTITQVTIARIFNWDVHQRRSKQPAMKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2DCT0</accession>
    <name>F2DCT0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123401548</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK361697">
        <property type="protein sequence ID" value="BAJ92901.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951310.1">
        <property type="nucleotide sequence ID" value="XM_045095375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DCT0"/>
    <dbReference type="SMR" id="F2DCT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557070"/>
    </dbReference>
    <dbReference type="GeneID" id="123401548"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0557070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401548"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF31">
        <property type="entry name" value="OS02G0139100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="72"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ92901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="206" mass="22839" checksum="E8483FBA28304A44" modified="2011-05-31" version="1">MEEAAATETVTETGTPEKSSYRYWVRQATGEAAPLPVPRKLDPAAANGVGGGNPNAPPSLGSVWNQAGTWEEKNLNSWANGRIKDLLGSLGSLDFSTGKASIDEVTKCSGDAFLVTVRNKKRVGYNYELSLRFKGEWLIKEEKKKVTGHIDIPEFSLGELDDLEAEVRFTDTLEWDDKSRISKDVKSFLSPIREKLGTFEQELKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DH32</accession>
    <name>F2DH32_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123443103</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363199">
        <property type="protein sequence ID" value="BAJ94403.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975297.1">
        <property type="nucleotide sequence ID" value="XM_045119362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DH32"/>
    <dbReference type="SMR" id="F2DH32"/>
    <dbReference type="FunCoup" id="F2DH32">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_57556.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0243720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0243720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0243720"/>
    </dbReference>
    <dbReference type="GeneID" id="123443103"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0202290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0202290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0202290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0243720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0243720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0243720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443103"/>
    <dbReference type="eggNOG" id="ENOG502QU5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011950_2_0_1"/>
    <dbReference type="OrthoDB" id="654309at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DH32">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36892">
        <property type="entry name" value="OS01G0201800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36892:SF10">
        <property type="entry name" value="OS01G0201900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="826"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ94403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0243720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="859" mass="93665" checksum="6D1B3497228E84BE" modified="2011-05-31" version="1">MAAAAFSIREYAASMRGGTAAEGRRLFGIEDLPPLRAPRCRWWADEVASTLAAAAPAGSPRRAPAKAKPPKKRSLSDLFAASPPLALPPASDTGCKEQTEVDGDEALRAIARRAKEEKKRKRKRQEEDETAVAAAAPESSGGREPEGNFAGRKEALENPDLFAGLDTHSSWKPEVSQQYREKISERRKQAKINNINKKADTRKHIDNNKANKAGKPRDLENFIPRNGILKCTKHTSVKMVKEKRGSSEGKEVIELCCKSVKRVKFSEADAILELPERESLCKMFSDAMASSSSSSSSSSSFTSTEGDKCITTESGSSHMPMEAFTKAKEANKNSDHEDSADKCISAETGTSHIPMEAFTKTIEANKNYDHEDSTEPGNREMSPPLIDLNMALPESTELDHRYDSYSVVLNLEHTHEETLSSVVELLDGRENRMNFSVDSQRLESEESTADLEIITNSSSASTFLHGEAIKVSDVNAAGPPLSLTELAETHRGCSNVSVKDTMTMSTTPCALPDHTFQDSFRQQQGWFSTSDKFSSWPSHESNVSHSKELNFHAELHVPHENGPSTGQTVRLMGKDLPVCTTRFFETAQKHTGTFTSDYLNLNVFLPQQGQPFLSLQGQNFPSDTVNSTSTIHASTYHASTSQARTTHDYSHPFPATNVLSGDQLPYEDRFVDFSNSQTNWSFQLGCPPPPKRGSAAFEQNSPSRRYYSDPITITEPPTAPPLPTTRQHGTPSSGFHANLPQPHVVHPVSSSVCHLNSVGFTFNHPDRVVQAPSNSIRDATFSARTTDNRVGSAVLGNSSASPSGRYVQMRSGPVKLTPGVRHVLVPNDGTGDDDSAPVYSCVPLGSRSTNGAGSRNKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DIJ6</accession>
    <name>F2DIJ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein O-GlcNAc transferase</fullName>
            <ecNumber evidence="3">2.4.1.255</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123426467</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="3"/>
    <dbReference type="EMBL" id="AK363714">
        <property type="protein sequence ID" value="BAJ94917.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966243.1">
        <property type="nucleotide sequence ID" value="XM_045110308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DIJ6"/>
    <dbReference type="SMR" id="F2DIJ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160170"/>
    </dbReference>
    <dbReference type="GeneID" id="123426467"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0132020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0132020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0132020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0160170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426467"/>
    <dbReference type="OrthoDB" id="421121at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000131">
        <property type="entry name" value="probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000181">
        <property type="entry name" value="probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000303">
        <property type="entry name" value="Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11380:FF:000002">
        <property type="entry name" value="probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11380:FF:000003">
        <property type="entry name" value="probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000070">
        <property type="entry name" value="probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="164"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="232"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="334"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="402"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="436"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="470"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="9">
        <location>
            <begin position="517"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="9">
        <location>
            <begin position="767"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ94917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="986" mass="110326" checksum="596983D3C974AD2C" modified="2011-05-31" version="1">MLSLRSDARHLAAPAVRGGDDVWLGVSGGAGPAPEGPAPAAAFLLPPVSLDARSKQLVGVNEEDHLALAHQSYKSGKYSQALEHGNAVYEKNPRRTDNLLLLGAIYFQLRDYDLCIAKNEEALAIDPQFAECYGNMANAWKEKGDIDLAIRYYLTAIQLRPNFCDAWSNLASAYTRKGRLNEAAQCCKQALVLNPRLVDAHSNLGNLMKAQGLVQEAYTCYLEAIRIDPHFAIAWSNLAGLFMEVGDLNKAMQYYKEAVKLKPSFADAHLNQGNVYKAMGMLQEAVACYQRALQARPDYAMAYGNLATIYYEQRQLDMAIHCYNQAIICDSRFVEAYNNMGNALKDAGRVEEAINCFQSCLVLQANHPQALTNLGNIYMEWNMISTAASFYKAAIAVTSGLSSPLNNLAVIYKQQGSYADAIACYTEVLRIDPTAADALVNRGNTFKEFGRVAEAIQDYIQAVTIRPNMAEAHANLASAYKDSGHQEAAIASYKQALCLRPDFPEVTCNLLHTLQSVCDWENRDTMFREVEEIIRRQIKMSLLPSVQPFHAIAYPIDPLLALEISRKYAVQCSLIASRFGLPPFVHPPPLPVKAEGKHGRLRVGYVSSDFGNHPLSHLMGSVFGMHDRDNVEVFCYALSQNDGTEWRQRIQAEAEHFIDVSAMTSDVIAKMINEDKIQVLINLNGYTKGARNEIFAMQPAPIQVSYMGFPGTTGASYIDYLVTDEFVSPTRYAHIYSEKLVHLPHCYFVNDYKQKNCDVLSPVCPHKRSDYGLPKDKFIFACFNQLYKMDPEIFDTWCNIVKRVPNSVLWLLRFPATGEMRVKAHAAARGVSPDQIIFTDVAMKHEHIRRSELADLFLDTPLCNAHTTGTDILWAGLPMITLPLEKMATRVAGSLCLATGLGDEMIVSSTKEYEDRAVELATNPAKLQALTNKLKEVRLTCPLFDTARWVRNLDRAYFKMWNIYCSGRHPEPFKVKEDDSEFPYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2E1M2</accession>
    <name>F2E1M2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY transcription factor WRKY51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123398892</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription factor. Interacts, when in complex with WRKY71, specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WRKY group II-a family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370043">
        <property type="protein sequence ID" value="BAK01244.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949273.1">
        <property type="nucleotide sequence ID" value="XM_045093338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E1M2"/>
    <dbReference type="SMR" id="F2E1M2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519920"/>
    </dbReference>
    <dbReference type="GeneID" id="123398892"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0432220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0432220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0432220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398892"/>
    <dbReference type="eggNOG" id="ENOG502QPQX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="687407at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E1M2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000004">
        <property type="entry name" value="WRKY transcription factor 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018872">
        <property type="entry name" value="Zn-cluster-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10533">
        <property type="entry name" value="Plant_zn_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="10">
        <location>
            <begin position="374"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAK01244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="48081" checksum="5880B47AA80B74ED" modified="2011-05-31" version="1">MEGVEEANRAAVVSCKRLVARLSLSAGDPFRLAAVAAETEEAVSRFGKVVNILSSRVGHARARVGRRSSPAGDPIARCLLEYHPPPPVPYCPPATATATATAPQLHGSSSSTPAPPTPLKQMPVPVVAAAPCAAGNGKVVAPADNCDADRDMFFDTPLLDLSGCSVTPASIAAAQINGSRVSAAPAAKPTDRGMFFQTPLLDLSGCGVTPASIAAAQINGSRVSAAPAASHPPPPPPPPPPQILFHHQIPQQQQQKKRPLEQQRPASSDNKRFHFEQKPASEKPFHIEIPAARSGKEPEVITFSFDNSVCTSSAATSFFTNMSSQLITMSESSACAPASRKAAHKADDDGKCHCPKKKKPREKRVVRMPAVSDKVADIPSDNYSWRKYGQKPIKGSPHPRGYYRCSSIKDCPARKHVERCRGDAGMLIVTYENDHNHAQPLDLATLTANSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F2E349</accession>
    <name>F2E349_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123428190</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370572">
        <property type="protein sequence ID" value="BAK01771.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968310.1">
        <property type="nucleotide sequence ID" value="XM_045112375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E349"/>
    <dbReference type="SMR" id="F2E349"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0205830"/>
    </dbReference>
    <dbReference type="GeneID" id="123428190"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0171240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0171240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0171240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0205830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0205830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428190"/>
    <dbReference type="HOGENOM" id="CLU_004747_7_1_1"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000183">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000001">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000006">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000004">
        <property type="entry name" value="Cullin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000002">
        <property type="entry name" value="cullin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000007">
        <property type="entry name" value="Cullin-3A like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="374"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK01771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="732" mass="85021" checksum="9AA181D4591E4A9B" modified="2011-05-31" version="1">MNPQRKRIPKIEPFKHRVEVDPKFFDKSWKKLDDAIREIYNHNASGLSFEELYRTAYNMVLHKHGGRLYDKLAENLKGHLREMGKLVEAAQGGLFLEELQRRWADHIKALQMIRDILMYMDRTFIPSSKKTPVFEHGLELWRDIVVRSPKIHGRLVDTLLELIHRERMGEMINRGLMRNTTKMLMELGSSVYQDDFERPFLEVSASFYSGESQQCIERCDCGEYLKNAEKRLAEESERVTLYMDAKTADKIANVVDKEMLTNHMQRLFLMENSGLVNMLINDKHEDLTRMYDLFKRVPDGHSSIRSVMASHVKETGKILVTDPERLRDPVDFVQRLLNEKDKYDEIVSVSFGNDKTFQNALNASFEHFINLNNRSPEFISLYVDDKLRKGVKGAANEEDIETVLDKVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTSSDEAERNMLVKLKTECGYQFTSKLESMFTDLKTSQDTMQSFYANLAGDVDGPTISVQILTTGSWPTQPCATCNLPPEILVVSEQFRAHYLGTHNGRRLTWQTNMGNADIKATFGDRKHELNVSTYQMCVLMLFNSTDTLTYKEIEQATAIPSVDLKRCLQSLACVKGKNVLRKEPMSKDISDSDSFHFNDKFTSKLVKVKIGTVVAQKESEPEKQETRHRVEEDRKPQIEAAIVRIMKSRRVLDHNSIVTEVTKQLQARFLPNPVVIKKRIESLIEREFLERDKVDRKLYRYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F2EHJ5</accession>
    <name>F2EHJ5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123441222</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375622">
        <property type="protein sequence ID" value="BAK06817.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973648.1">
        <property type="nucleotide sequence ID" value="XM_045117713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EHJ5"/>
    <dbReference type="SMR" id="F2EHJ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330520"/>
    </dbReference>
    <dbReference type="GeneID" id="123441222"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0275280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0275280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0275280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0330520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441222"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="538"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK06817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="657" mass="74030" checksum="2ADCA296EEE66559" modified="2011-05-31" version="1">MSMSGADFNGVGEDPRVFRNICRDRILKDMLKPDKDKETKSSWKVLIMDKFTVRIMGYACKMAEITDAGVSLVEDLFKRREPMPSMDVIYFVQPLKENVIMLLSDMSGRCPLYRKAYIFFSSPIPKDLVTYIKNDSSVIPRIGALREMNLEFFAIDMQGFVTDHDMALTDLYGANENNSKKFNDTISTMACRIATTFASLKEFPTVRYRAPKSADPSTAPKFDMVPKWLATATWEIVSKYKSTIPEFPQKETCELLVLDRPIDQIAPVIHEWTYDAMCHDLLEMDGNKYIYEVSKGDAEPEKKEALLEDQDPLWIELRHTHIADASERLYEKMNTFVSKNKAAQLHSRDGGEISTTDLQKIVQALPQYGEQVDKLTLHIDIAGKINRCIREFGLRDLGQLEQDLVFGDAGAKEVINMLRSKQNLSEENKLRLLIIYAIVCPDKFEGDKGDKLMQLAKLPSDDMKAINSLRYLVSSDAKKAARAGGFSLKFDAQKKKNTGVRTERQDGEEGWALSRFFPLIEELVEKLSKGELPLNEYPSLSQPSSTSQGTAESGSAPKPAQNPQPMSRRSRRTPQWAKGRNSDDGQSSDSSVLRHASGDFKRLGNRIFVFIVGGATRSELRVAHKLTMKLKREIVLGSSSIDNPPQFISKLKALGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>F2EK03</accession>
    <name>F2EK03_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="6">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123449199</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Flower</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Extracellular space</location>
            <location evidence="6">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="6"/>
    <dbReference type="EMBL" id="AK376480">
        <property type="protein sequence ID" value="BAK07675.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982262.1">
        <property type="nucleotide sequence ID" value="XM_045126327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EK03"/>
    <dbReference type="SMR" id="F2EK03"/>
    <dbReference type="PaxDb" id="4513-MLOC_60000.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0393250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0393250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0393250"/>
    </dbReference>
    <dbReference type="GeneID" id="123449199"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0327160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0327160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0327160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0393250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0393250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0393250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449199"/>
    <dbReference type="eggNOG" id="ENOG502QVQI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048041_1_1_1"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EK03">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019646" description="Xyloglucan endotransglucosylase/hydrolase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="8">
        <location>
            <begin position="14"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK07675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0393250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="325" mass="36298" checksum="4E47A51419E3C80F" modified="2011-05-31" version="1">MACHFLLAVLLASSSWVAASSGAAADDVMVPRPTTAAALTFREGYTQLFGDSNLRLHGDGKRVHISLDERTGSGFASQGAYFHGFFSASIKLPSDYAAGVVVAFYMSNGDVYEKTHDELDFEFLGNVRGKEWRVQTNVYGDGSTAVGREERYGLWFDPTHDFHRYAILWTNRTIVFYVDGTPIREVVRSEAMGAQFPSKPMSLYATIWDGSSWATSGGRYKVEYKYAPYVAEFTDLELRGCASHDRAQPASCEPEGMPARQRAAMERVRARHMTYGYCYDRARYPAPLPECRVGAEAAMYLPSGEARSSDRRRHGKRHRRADSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>M0USM0</accession>
    <name>M0USM0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447417</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable anion transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sodium/anion cotransporter (TC 2.A.1.14) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979951.1">
        <property type="nucleotide sequence ID" value="XM_045124016.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979958.1">
        <property type="nucleotide sequence ID" value="XM_045124023.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979966.1">
        <property type="nucleotide sequence ID" value="XM_045124031.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979974.1">
        <property type="nucleotide sequence ID" value="XM_045124039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0USM0"/>
    <dbReference type="SMR" id="M0USM0"/>
    <dbReference type="FunCoup" id="M0USM0">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0USM0"/>
    <dbReference type="PaxDb" id="4513-MLOC_13828.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078230"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078230.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078230"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078230.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078230.3.CDS3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078230"/>
    </dbReference>
    <dbReference type="GeneID" id="123447417"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0063620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0063620.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0063620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078230.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078230.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078230.3.CDS3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447417"/>
    <dbReference type="eggNOG" id="KOG2532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="LITFWMP"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0USM0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17380">
        <property type="entry name" value="MFS_SLC17A9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000199">
        <property type="entry name" value="Probable anion transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000220">
        <property type="entry name" value="Probable anion transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044777">
        <property type="entry name" value="SLC17A9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF445">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="12"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078230.3.CDS3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="436" mass="47651" checksum="8639621F34EF4D4E" modified="2013-04-03" version="1">MKRMKIPKRYVIVLLTFICTNVCYIERVGFSIAYTVAADAINVNQANKGLILSMFYYGYVLSQIPGGWAAQRLGGRRVLLLSFLLWSLICGLIPLDPNRVVILVLSRLFVGVAQGFIFPAIHTVLAQWVPPQERSRSVSLTTSGMYLGAAGGMLFFPSLVKHMGPQYVFFVEAVLGVAWSVIWLKFSSEPPRTDLPKVAMPKVASREKIKALAGGVVAPRTVKIPWRKIIFSLPVWAIVVNNFTFHYALYVIMNWLPTYFELALKLSLQDMGSSKMLPYFNMFIFSNIGGVVADHLITKRILSVTKTRKLLNTIGFVVAAVALMALPSFGTPSGTVICSSVSLGFLALGRAGFAVNHMDVAPKFAGIVMGVSNTAGTLAGIVGVGLTGNILEAAKASNMDLTSSETWKTVFFVPAYLCIFSSVIFLIFSTGEKIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>M0VSZ1</accession>
    <name>M0VSZ1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0VSZ1"/>
    <dbReference type="SMR" id="M0VSZ1"/>
    <dbReference type="PaxDb" id="4513-MLOC_3698.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0509230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0509230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0509230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0614170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614170"/>
    </dbReference>
    <dbReference type="OMA" id="DSMHATG"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VSZ1">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20169" checksum="BB497D6630F7D6AD" modified="2013-04-03" version="1">MEASGLVFLAALNSFPALTRSSLNSNTAGGNHALVSSWLSSLLPFCCCSCCCLPRRSSLSMEMDSVVSLGYNSKFEGSGFVMFTSQPAFLVMTCEHVVGSYKSIQVTFPGKTKWYSAGVRWTDKDADLALLRFAPDGDCSRCAALQFADPNTTALNSGNVEMIAFHATSPGRLLYPGVFDGCITAGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>M0W2S9</accession>
    <name>M0W2S9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123407225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956246.1">
        <property type="nucleotide sequence ID" value="XM_045100311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W2S9"/>
    <dbReference type="FunCoup" id="M0W2S9">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W2S9"/>
    <dbReference type="PaxDb" id="4513-MLOC_4183.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643260"/>
    </dbReference>
    <dbReference type="GeneID" id="123407225"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0534510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0534510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0534510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0643260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407225"/>
    <dbReference type="eggNOG" id="ENOG502QSA0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W2S9"/>
    <dbReference type="OMA" id="ITNCCIQ"/>
    <dbReference type="OrthoDB" id="1878759at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038920">
        <property type="entry name" value="At3g05675-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058039">
        <property type="entry name" value="At3g05675-like_ankyrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060:SF7">
        <property type="entry name" value="OS06G0129200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060">
        <property type="entry name" value="OSJNBA0011J08.25 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25553">
        <property type="entry name" value="BTB-POZ_ANK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="437" mass="49032" checksum="8704BF10105DAF14" modified="2013-04-03" version="1">METVNAVDGYKFADESTSDVRVCFRRTGSNEQQPERFPCHSSILSDRSKYFADLLGRRDVPFGNNNCIEVECPRAEYDHYVKLLKFMYLSRESIEEEFTSVKSALGVLRAATSLKSEFIAETCIGYLESASWDEKEEEEILQFAQTLGPEAAAPLLARLQAPSANAVKTVFISAVHFATSMEISSAPVFDDLKTAAQEQIDFMLHDGDDPAIVMMDEDVRSVFREGLTKLLSTLRTGLDLLASEFDQLPEQAEQRILHSLVDIDWITTVLTKIELMNEFVSGWLEISDHVISVVQDDKYSSGLWAVKRKLIEVTGKALDAVGYGSVVLPSTSRTHLVKTWLPYIRTTKRLLDGKTKDDAFPQMDANLCQNIESAIVSMVLALPSGDQSDILSDWMQKADQFRYPDLTEAFEMWCYRSKTAIRRLNGAIDKAGNPISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0WVE5</accession>
    <name>M0WVE5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0WVE5"/>
    <dbReference type="SMR" id="M0WVE5"/>
    <dbReference type="FunCoup" id="M0WVE5">
        <property type="interactions" value="1042"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WVE5"/>
    <dbReference type="PaxDb" id="4513-MLOC_54973.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0162880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0162880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0162880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0196300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196300"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WVE5"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WVE5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="333"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="587" mass="63955" checksum="80752A0744256E4D" modified="2013-04-03" version="1">MAKSSSATASGTKKRKSKSGALTQEEVKSLGLELLSSRAHLNHAPTLLALLSPTAPLSIALEALISLQSFFEPLLPSIPSASAAAAVAAGGASDDPELVFGAWLRQRFEEFVAALVELSVSPDSGDAIREVALDAFMDFVKLGKDGSFNSAIYHKFLHAVVHATDSIDAVLELLGSKYAKYADVCFFTYTSLDKIANSLGSQTTGSGKDGLQNGDDGAKDRSAICVHNVYNILVHIPALDFKKETKFDMWSTVGLSSKGEKDTSEGSSATRISKKLKLKFTKAWLAFLKLPLPLDVYKEVLATLHQNVIPSMSNPAILCDFLTTSYDIGGVISVMALSGLFILMTQHQLEYPKFYDKLYALLTPAVFMAKHRSVFLQLLDACLKSSYLQAYLAASFAKRLSRLALSVPPAGALIIIALIHNLLRRHPSINFLVHWEVAQDDGEASRPKKIGADPFNNEETDPAKSGAMRSSLWEIDTLRHHYTPAVSRFVVSLETDLTIRAKTMEMKITDFSSGSYATVFRDEARRRIKQVPLAFYRSTPTSLFQESDFPGWTFGCQSNTGAQASVEGNAVHTLETVDASPAKRSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>M0X1H2</accession>
    <name>M0X1H2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123428019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968106.1">
        <property type="nucleotide sequence ID" value="XM_045112171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0X1H2"/>
    <dbReference type="SMR" id="M0X1H2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0201200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0201200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0201200"/>
    </dbReference>
    <dbReference type="GeneID" id="123428019"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0167180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0167180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0167180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0201200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0201200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0201200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0X1H2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00043">
        <property type="entry name" value="rRNA maturation RNase YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="ENDORIBONUCLEASE YBEY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986:SF2">
        <property type="entry name" value="OS02G0227000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0201200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="524" mass="58939" checksum="5D3A604CA6B074EE" modified="2013-04-03" version="1">MARLHHLLSRALASNHLLRPPPLPFSARPTLPLHPQPPPPPHPHGRSPPPFPSAASRHYASSIPRRRRRGSAPTMFPRRRRARRKGPGELSVQIGIEEALPDDPLILSIAETLRTDAGKAMKLAFHNLGNSEYKTRDPCISNVDEYDSVEVSLLLCDDDFIRKLNKEWRDEDHATDVLSMSQHIPGLQIPVLQLGDLVISVETARRQAEERGHALLDEIRILMVHGLLHLLGFDHELSKEAEEEMEQEEEHILNTLEWKGKGLIKSAYHFCTDMDHSENSDANRDIEKRSLREGHQPKLTHIVCDIDGHLHSESIESLREAISRGVTVIMLTGKTRASTIATFKLLNMEGRGDFISENSPGVFLQGSLVYGEHGQLIYRANLDVDICKEACLYSMNHKIPLVAYCEEQCLTLFEHPSVDLLHTVHHETKVKVMPSVEDLLEYSSIQKLLFLGNTDEDFSVLTRHWSELTEGKACVIKGQLNALEIVPLNASKGGGIRVLLDHLGITEDSDLEVVGDYTRWLSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287KBF4</accession>
    <name>A0A287KBF4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123442888</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974999.1">
        <property type="nucleotide sequence ID" value="XM_045119064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287KBF4"/>
    <dbReference type="SMR" id="A0A287KBF4"/>
    <dbReference type="FunCoup" id="A0A287KBF4">
        <property type="interactions" value="1428"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287KBF4"/>
    <dbReference type="PaxDb" id="4513-MLOC_55326.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236160"/>
    </dbReference>
    <dbReference type="GeneID" id="123442888"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442888"/>
    <dbReference type="InParanoid" id="A0A287KBF4"/>
    <dbReference type="OMA" id="ISMAHRY"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287KBF4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000002">
        <property type="entry name" value="Cyclin/Brf1-like TBP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000066">
        <property type="entry name" value="Transcription factor IIIB subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="77"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="178"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="530"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="582" mass="63747" checksum="58074987E4585067" modified="2017-11-22" version="1">MVYCSHCADYCPFIKDPDKGYICCGTCGKVIDQEIFTDEPTFFKDSSGASRLAGNILSSIESGSSLSHERTLMKGRDEIWQIVTSLHVGGGDTIIDMAHKFYTLAVDHNFTRGRRTTHVAAACLYIACRQSKKAYLLIDFSDYLKISVYVLGAVFLQLCQVLLLAEHPIVQKLIDPSLFIHRFTERLLGKRDNAVSDTALRIVASMKRDWMQTGRKPSGLCGAALYIAALSHGYNYTKSNIVAVVHVCEATLTKRLIEFENTDSGSLTIEDFLAKADEEPVSIFSPIPGEVLCKHKDKAAEHFAHGLCEKCYNKFTKLSGGLEGGADPPAFQRAEKQRLEAAKKAEEAAAIKEGMLGESICEIQNSDAEYNIMSTTEDSVGDKSATIGSGEIGKDYVASKDPEVGGENGKADADPESFSDIDDVEVDGYLHNEEETQYKKIIWEEMNKEYLEEQAAKEALAAELAARGVGVGEGQQKKRRRNEDMKNSTPAETPAEATYNMLKRKGLGSKINVEAVGGLYNTKDEASEVNEKGDMGFDGEHAHDTGDGETLEGGYDYADYNNDGYADGGDAGAYEDYDGIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MJH1</accession>
    <name>A0A287MJH1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucan endo-1,3-beta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123440915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973391.1">
        <property type="nucleotide sequence ID" value="XM_045117456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287MJH1"/>
    <dbReference type="SMR" id="A0A287MJH1"/>
    <dbReference type="STRING" id="112509.A0A287MJH1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0318900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0318900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0318900"/>
    </dbReference>
    <dbReference type="GeneID" id="123440915"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0266100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0266100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0266100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0318900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0318900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0318900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440915"/>
    <dbReference type="InParanoid" id="A0A287MJH1"/>
    <dbReference type="OMA" id="CGLLMAT"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287MJH1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0318900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="36446" checksum="51100A18A3467E20" modified="2017-11-22" version="1">MARQRTFSSSTLAVALVVGILASIPIEVQSIGVCNGMIGDSQSLPPRADVVQFYKTNGISAMRIYAPDPETLRALDVDGTGIDLMMDVGNGNLSALASDPGLAASWVQENVLPYPRVSIKYIAAGNEVVGGDTQNIVPAINNLNNALAKAGITSVKVSTAVKMDVLSSSSPPSAGVFKDAYMTEVTQLLKSTGAPLLANVYPYFAKRDTPSIDLSFALFQQSPNPVHDDGNGLTYTNLFDAMVDALYTAMEKADASDVPIVVSESGWPSAGDDLATLTNAQTYNQNLIDHVGKGTPKRPVPLETYIFAMFNENQKEGPDTERNFGLFNGPDKTPVYPIRFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W3N6</accession>
    <name>A0A8I6W3N6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NBS-LRR resistance-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W3N6"/>
    <dbReference type="SMR" id="A0A8I6W3N6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0002940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0002940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0002940"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0003000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0003000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0003000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0002940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0002940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0002940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0003000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0003000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0003000"/>
    </dbReference>
    <dbReference type="OMA" id="HGMYNAS"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF23">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="2">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="107"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0002940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="54187" checksum="DE3076E5BEBD1CD0" modified="2022-05-25" version="1">MRQWITAGFITEKDESKTLEEVAEGYLVELVNRSLLRVVERNHTGRLKCCQMHDVIRLLALNKAKEECFGKGYNGSGGTGAFSIEGAGRISVQCGNLEQLSRSCARHLRALHVFERYINVDLLKPIITSSNLLSTLDLQGTNIKMLPNEVFNLFNLRYLGLRNTEVESLPEVVGRLQNLEVLDALESKLMYLPKSIVKLRKLRYLYVCTPATSETVTIGGVKVPNGMQHLAGLRALQSIKATPEFLREVAALTELRTFDVCDVRREHSADLSNAITKMSHLVHLGIAVAEDELLRLEGLNLPPTLSMLTLGGQLEKTKMPQLFSSWSHLNSLTRLHLEFSNIDEQTLSCLFVLRGLRFLYLLKAFEGKRLDFYAGSFPKLRHLLIGYASQLNQVGIEEGAMQNLVELLFRDCPELKFLPDGIEHLAALEKLVLQDTSEELIEKLRQKRDSDECSQDVMKINHIRNVTVVLSHKGLVERIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W6I3</accession>
    <name>A0A8I6W6I3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7731 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123412321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961215.1">
        <property type="nucleotide sequence ID" value="XM_045105280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W6I3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061520"/>
    </dbReference>
    <dbReference type="GeneID" id="123412321"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0048900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0048900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0048900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0061520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412321"/>
    <dbReference type="OMA" id="TCIWLLR"/>
    <dbReference type="OrthoDB" id="1843925at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056633">
        <property type="entry name" value="DUF7731"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34366:SF9">
        <property type="entry name" value="OS03G0304200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34366">
        <property type="entry name" value="OS07G0289901 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24865">
        <property type="entry name" value="DUF7731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035173474" description="DUF7731 domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7731" evidence="3">
        <location>
            <begin position="58"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="28835" checksum="A0484A1C7600855C" modified="2022-05-25" version="1">MASFKLTHVCFIVVMFSALFSCCRGQGGGGGGGAGAATAGGGGGSGIGAVVPGTQDSVQIVAQAALCFDNRPVLTGCLQAMGINSSGTGAGMPPPAPGSAASTAIMCSPPCFGHVSMMMSCVNAIFGNFVSYNPGLMQGVQAVFQMSCGNVNGPGGAGAGAGGAPGGGAAGGAGGGPAGNAAGAGGAGASGGVGGGAPGGIPNGIGGPGGIPNSIGGAAGGGSGAAGASGTTNIIGGAAGGGAGAGAGGASSGGVGGNDTTNGGAAIGTVGSSNITSVSPNAGSHVAVSNQSRPTSSADGPTLSLKAACSSALVIWAGASLMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLL0</accession>
    <name>A0A8I6WLL0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449710</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982960.1">
        <property type="nucleotide sequence ID" value="XM_045127025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLL0"/>
    <dbReference type="SMR" id="A0A8I6WLL0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085420"/>
    </dbReference>
    <dbReference type="GeneID" id="123449710"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0070250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0070250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0070250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0085420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0085420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0085420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449710"/>
    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1020:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.50:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="76"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0085420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="211" mass="24299" checksum="D213090C2915FC27" modified="2022-05-25" version="1">MAGQSDPHLSLFSPSEVEFVAEDEIVEIVPNIRMEALNMICGDFGPFFPQIPSKVPLWLAVALKRRGKCTIRAPEWMTVERLTQVLDAERESPREFQPLPFHYIEISKLLFDHARDDITDAYLVRSLIEDIRDVRFHKVESGLETISGRTHAVKLKNLSAMEVNIVRPFMVRTLQAFYKHDSPQMIQQADNTGSRSTPVTDRGPRRDLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM46</accession>
    <name>A0A8I6WM46_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123402754</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952645.1">
        <property type="nucleotide sequence ID" value="XM_045096710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WM46"/>
    <dbReference type="SMR" id="A0A8I6WM46"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092550"/>
    </dbReference>
    <dbReference type="GeneID" id="123402754"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0076210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0076210.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0076210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0092550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402754"/>
    <dbReference type="OMA" id="KGCCMCL"/>
    <dbReference type="OrthoDB" id="713501at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <lineage>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="9">subsp. vulgare</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A8I6WSP5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0159940.1">
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    </dbReference>
    <dbReference type="GeneID" id="123426456"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0159940.1">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032116">
        <property type="term" value="C:SMC loading complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000614">
        <property type="entry name" value="Sister chromatid cohesion protein SCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21394">
        <property type="entry name" value="MAU2 CHROMATID COHESION FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="689"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="707"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0159940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="727" mass="80318" checksum="BB924857A0C01071" modified="2022-05-25" version="1">MPSAGPSMSAADGLLALAEEAERRRDFSTATSCLESALSPPHAASLLPLAEARARMRLAGLLLARNKGLANAKAHLERALLVLNPLPSAPPRLKLLAHSLLANVYGLLGAVPSQKHALRRGLTLLASASASGLLPSGPALLWTSNFQAQLASALVADGDAASALSTLSAGAAAAADLESPQLDLFFAATALHVHLLCWEDNAAVEDAVARVSRLWDALTAEQKEHWVGLFFYTELLQTFYLLRICDYKAASKHVERMDIAVKNEMERGRRIKELGTELSAVEGTLAQTMLKERERVALAHKQGQLRAQLQALCGYDTLNDVLDYGDKLLLAPPPMHGEWLPRTAVFVLVDLMVVMVSRPKGIFKECGKRIHSGLQLIHEELSKLGIVDGVTEGDLEHSTMWTAGLYLMLLLQFLENNVAVELTRSEFVEAQEALAQMKNWFTRFPTILQGCESTIEMLRGQYAHSVGCFDEAAFHFLEALKLTENKSMQSMCQVYAAVSYICKGDAESSSEALELIGPAYRTMDSFVGVREKTCIIFVYGLLLMRQHNPQEARVRLASGLRIAHQQLGNIQLVSQYLTILGTLALQLHDTGQAREILKSSLTLAKTLYDIPTQIWILSVFTELYRELEEKENEMENSEYVSKKEIELQRRLAEARSRACHQELVEKVRIEAEPLHDLFQKHNDMSGLPVNDDLDIPESVGLSTPQPSSVKRLVDSSSVRRSTRRRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WT84</accession>
    <name>A0A8I6WT84_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123425736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WT84"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0136340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0136340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0136340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0136340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0136340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0136340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264:SF3">
        <property type="entry name" value="OS07G0554100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264">
        <property type="entry name" value="OS07G0554500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0136340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45414" checksum="B73F2B4FB04AA773" modified="2022-05-25" version="1">MAEMSSLPVTDDLLAEIFLLLPTPADLVRASAACVAFRRLVTDRPFLRRFRSLHARPFLGFLNHKGFHPARPPHTSAPAASAVSLAADFSFSFLPSHDSWIVRDVRDGRVLLDRTPEDVAGEESPVFTELAVCDPLHRRCVQLPPIPDDLAVSVEHPLRVEFERWCEPFLAPPGEEATEETSFRVIWMAQCKTKLVAFVFSSSTGEWRAVASQSWGDLLVGTGVSTASSKSPVFFGRQYACGCFYWVMDWRQKLLMLDTQMMEFSIADLPPGYRRPPIAIVDAGEGRPGMFAVRENDAVGTFDLYYTIRRNEGQSFNQWQMEKTIPLESGYRYYLRGATERYLLLLRSEDDSASSSSLEMSGTECFSLDVKTLQLESICRLKHHILRAHIYTNFPPSLSSQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUC8</accession>
    <name>A0A8I6WUC8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123412593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961482.1">
        <property type="nucleotide sequence ID" value="XM_045105547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUC8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063310"/>
    </dbReference>
    <dbReference type="GeneID" id="123412593"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412593"/>
    <dbReference type="OrthoDB" id="650089at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF292">
        <property type="entry name" value="OS02G0262500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="302" mass="34857" checksum="FA45C7E3B165E1E2" modified="2022-05-25" version="1">MYRKKTLWTASYLSDGFFLGMRSNQRSESLNSSLHLHLDYGMTIVDLVVHYENCIDRLRENEAEDDCTANQSLPPSITAYKAIEDHDALVFTPANFYILQKDLLKLAELEIFETLVGVQRSTYIVTWKNNHKLRYDVIYEPSNLDETISYSYGMMVRKGLPSKHIFFVLVNVLKLSEIPNCCVLRRLTKHARHGLPAQRRSDLFGWGWSGEEQRSWYSRLSIKDAQAFHVASNDTFLFDDLMKCLDNIILQRKIPLEEIIGQRRYKRMIGNAPEESGGIIGDPPNIRSKGAPKKPRKVVQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WWI8</accession>
    <name>A0A8I6WWI8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123440935</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3 9">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044973407.1">
        <property type="nucleotide sequence ID" value="XM_045117472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WWI8"/>
    <dbReference type="SMR" id="A0A8I6WWI8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0320140.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0320140"/>
    </dbReference>
    <dbReference type="GeneID" id="123440935"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0267120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0267120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0267120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0320140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0320140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0320140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440935"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000012">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF708">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="91"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0320140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="226" mass="25272" checksum="DB5439C2EF0AEB20" modified="2022-05-25" version="1">MGEPEPPVKLISSFGSTFSHRAEVALRLKRVSYELVVEDLRNKSQLLLTHNPVHKKVPVLLHGDRHAISESLVIIEYVDEAFAGRPLLPTDPHARATARFWAHFIDEKCMKSLWAALWMEPSEAQRRSVAEAKGSLALLEEHLGERSFFGGDSVGLVDIAASVLAHWLGVMEQISGVTVVTDAEFPTLCHWARRYASDEDVKTCLPERGELIAEEMFRSMAMTTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZ60</accession>
    <name>A0A8I6WZ60_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZ60"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270880.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270880"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0270880"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF24">
        <property type="entry name" value="TTF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="258" mass="30448" checksum="C16359D2CEEE5A07" modified="2022-05-25" version="1">MAFFVQPLLKKMSNGEVCDRRWLVFSKQANKVFCFCCKLFNSNNCKSTLGHDRFCDWWHISERLKEHEASVDHITNMNCWNELRNRLSKHETIDKELQHQITKEKECIRQVLLRIFSIVKFLGKHNLGSRVSSEQFYDDSNGNFLVCVEMVVDFDLVMQYHLWRIQNKDIHYHYLSHKIQNELISLMTADIRNSIIKIVKVAKYFSVILDCAPFVSHQEQVSLLVRCVHMYGGKTQIVEYFLGFLVVHISFGNFQFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X001</accession>
    <name>A0A8I6X001_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine desulfurase</fullName>
            <ecNumber evidence="2">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123448081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6X001"/>
    <dbReference type="SMR" id="A0A8I6X001"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0352920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0352920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0352920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0294400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0294400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0294400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0352920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0352920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0352920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="152"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0352920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="549" mass="59048" checksum="EF415814219670B6" modified="2022-05-25" version="1">MATHLAWSTPPRVVEEGAIVERQGSASAGGGGGLGDGSGCYTQAAPLHHGERKHTARLWLIQPTETARMATAAAVASLRYFPSSVRNCSYPGAISGGGGGGAVVTFSPRRGRCATAAVAAPSREAEPASSLGDLTRVDFPILDQEFDGNKLVYFDNGATSQKPSHVMKALDDYYRFYNSNVHRGIHALSAKATDAYESARRKVANFVNAADSREIIFTRNATEAINLVAYSWGLSNLKEGDEIILTVAEHHSAIVPWQFVSQKTGAVLKYVGLTKEEVPDIEQLKGLLSSKTKIVVVHHVSNVLGSMLPIEEVVSCANKVGAKVLVDACQSVPHMPVDVQKLGADFLVASSHKMCGPTGVGFLHGKFEILSSMEPFLGGGEMIADVLEDKSTYAEPPSRFEAGTPAIGEAIGLGVAIDYLSHFGMQRIHEYEKELGSYLYESLLSVPNVRIYGPAPSQSDHRAPLCSFNVENVHPTDIAEILDLQHGIAIRSGHHCAQILHRTLGINASARASLHFYNTKEEVDVFIHGLKATIDFLTSQHLFSSVSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0E4</accession>
    <name>A0A8I6X0E4_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123444331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976951.1">
        <property type="nucleotide sequence ID" value="XM_045121016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0E4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286380"/>
    </dbReference>
    <dbReference type="GeneID" id="123444331"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0237710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0237710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0237710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0286380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444331"/>
    <dbReference type="OrthoDB" id="678085at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36892:SF7">
        <property type="entry name" value="HVA22-LIKE PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36892">
        <property type="entry name" value="OS01G0201800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="904"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1228" mass="134558" checksum="34C8905C6FD4361C" modified="2022-05-25" version="1">MAAASAAASAAAAEAFSIRGFASMMRAVDAAKCWPFGGVEDGTTETPQLPPMDPTPRSRWWADGVAAARARPGFCAAGRDFAVGGGMEDGLRKATKRKGSRGSGGAGRANKRQRALQSLFYLKHKERTSKPQPTSRLLQLMLHKGLLRKRKGCTIPALRELVMRKKLQERQNQMSTHRNSMKKQSGRAMDHEQSIKISRPIDYPVNPGCEVVNHVACPPKDDIFGDLPLLESSKVMFRTGVDILPTNIEDSFVENQNGSDAISETEPLKLMSFVDISKQTSLHVEDLVKKEQTPDKGSTCIFPNDAGRSHSSSAEFDGLLNHTSVAKTSLPEMQLKSTNVPAYCNEGAKSGSSDRPQGCFYMNTNCFQEMERPGTSSVAVRTRTEAIKKEDAAVHGLNNTGVTVEKTCLPEMPLKSTHVPALSSYCNGRAESGCSNRPKGPFHTNTNCFQEMKRLGTSSVAVRTRTEAITEDRDAAVHGLNNTTVTMAKTCLPEMQLKSTHVPALSSYCNEGAKSGSSNRPQGCFYTNTKFFQEMTRPGTSSVAVRTRTGAIEKDGDPAVHDINHTSITVAKTCLPEVQRKSTHVPTLSSYCNEGAKSGSSNHPQSCFYSNTNRFQEMTRPGTSSVAVRTRTEAIEKDRDTAAHGKKSTDISGRLVPTEYYPSCEGSLSPSVISQRTFNAGINADGMSSCRIVPAQEYVPASNPCKVASNVHHGSRKSVDTCTSLSIDDQGSWHSKFYPARSPASINLPFMKLPGLEKMEISSYDPRTGETNFVNGRSMNTVRYQQEKQVGGMASTIQGQNKIGFSDSQAGKKDPDGFVRRDNCHLHQPTMRLMGKTVSLSKGRMEQEVPTTGKVWIDKNIMEGDHPSSISCQLPPKRVFPYHDSVMLRTRVHESSDTLPRIHNNNNNSNNNNNNNNLPRIPSSNFAEARPIVTDAQNHRPPQTNIVSSAVKDWTWNSGSQFGQQAQVNKESIIGNSRTRHTELSQPPKLTSIPRNQYLQSGNPASSMYAEHTFVGSAVNRSSSSFPQWSLNTSMRGEYQNPTSFVYEDPRSVPAHQPPCQVAGANLFSASNIFSHDYGTKNAESRNSYQRAHPSLPTSAASRFHDYCTRNADSRYSYQGAHPSLSTSMASKSISAISLTCTGGVLNMDGRKGVSMVDERGKRPGCADNVSQQPAKRQLVSDKQELSTSMFPSMEKYSFGWSLNDTVGPRMLDFSNKNSRTCDKNIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X245</accession>
    <name>A0A8I6X245_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X245"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0276960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28957" checksum="7DB410A04AFC658B" modified="2022-05-25" version="1">MPGRPSRQAVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEEPIHWSYGLGVTVDMTVQDAAYSMLTIMRARLALLQNLEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALCMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGPQGSQARVFPDPHVELPSHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X677</accession>
    <name>A0A8I6X677_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X677"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0310080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="64" mass="6454" checksum="65D1C4A1FB7507E4" modified="2022-05-25" version="1">MAVTPGITQGASENSGMSRVIGNIGPLSSGPDMVPLNANMGTFLPGADNSTELVLISFSLCTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBL4</accession>
    <name>A0A8I6XBL4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Co-chaperone protein p23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123446914</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as a co-chaperone for HSP90.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with HSP90 in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979394.1">
        <property type="nucleotide sequence ID" value="XM_045123459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBL4"/>
    <dbReference type="SMR" id="A0A8I6XBL4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333020"/>
    </dbReference>
    <dbReference type="GeneID" id="123446914"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446914"/>
    <dbReference type="OMA" id="LEDWNAN"/>
    <dbReference type="OrthoDB" id="1564555at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000030">
        <property type="entry name" value="Co-chaperone protein p23-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="17052" checksum="7E867B9E23A0D808" modified="2022-05-25" version="1">MSRQPEVLWAQRSEKVYLTISVPDAEDVVIKTEPQGIFSFSAVAHGESFSFTLELFDSILPEGSKTKTKVGSRNIICSIQKDKKCWWKRLLKSEAKHPYIKVDWNKWCDEDEESENSGSDDDFDGVENDENDADDGMLYLPDLEKLRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBS8</accession>
    <name>A0A8I6XBS8_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBS8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0237740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0237740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0237740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0237740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0237740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0237740"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045501">
        <property type="entry name" value="DUF6490"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46610:SF18">
        <property type="entry name" value="OS01G0183850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46610">
        <property type="entry name" value="OS05G0181300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0237740.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="188" mass="20664" checksum="A304D90500125D1D" modified="2022-05-25" version="1">MEPTQTLVARYTPPPKTVQLGQGDGSAQPGCGWAFWAVLSLVTGHLAWGLYRARRSVHDLAFVIMAYYPTYFCLWGLYVCLRRHELLRCDDDPAAAPKRRQARFRVWAASLTVGGMIAVQLAGATPDLALKFTLIALGCLAILLAGYCLYAGGRGAPAEAGSRPEKELHEVSTEHRVSTLKLEVHPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XD41</accession>
    <name>A0A8I6XD41_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XD41"/>
    <dbReference type="SMR" id="A0A8I6XD41"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0253100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303972" description="3'-5' exonuclease domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="82"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="28946" checksum="1F04B54CBE00F431" modified="2022-05-25" version="1">MKSTNRSRVAISLFLLLSRSPSLFFSSLLPFSSSTMPTSFHKVLAHGMTMLDVVYTNLSSEVPFFLQQLKEKWFDNAVDHEKFLGLDLEYTADQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSGITFASVDIRNDKLKMRHNFGIEIPAGCLVDLQTIFRLRHDRTSMAHMAVALIDDSYADMKTSFPKSQHKLWEKGPLYDINIEYAAKDAYVSYELYRKIRVVHYGQRHLEEGGHSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDH5</accession>
    <name>A0A8I6XDH5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123443542</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975893.1">
        <property type="nucleotide sequence ID" value="XM_045119958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDH5"/>
    <dbReference type="SMR" id="A0A8I6XDH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0257920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0257920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0257920"/>
    </dbReference>
    <dbReference type="GeneID" id="123443542"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0214120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0214120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0214120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0257920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0257920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0257920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443542"/>
    <dbReference type="OMA" id="DEMIREP"/>
    <dbReference type="OrthoDB" id="727752at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Calmodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calmodulin"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF53">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13499">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0257920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="16804" checksum="A4C4B5516677509B" modified="2022-05-25" version="1">MADQLTDDQIAEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMVREADVDGDGQINYDEFVKVMMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEC7</accession>
    <name>A0A8I6XEC7_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123449408</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982558.1">
        <property type="nucleotide sequence ID" value="XM_045126623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEC7"/>
    <dbReference type="SMR" id="A0A8I6XEC7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398930"/>
    </dbReference>
    <dbReference type="GeneID" id="123449408"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0331880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0331880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0331880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0398930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449408"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF6">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="344"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="379"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="414"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="519"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="554"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="589"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="624"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="659"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="710"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="789" mass="86864" checksum="6398EE870409EBF1" modified="2022-05-25" version="1">MDTPPSTSASAATGSDPDSALVAAVADALVSASRLPAPVPMPTLLAAYLPRLTASHHPRVLSIAASNPALASPEPLLAYRSFLSPPSCLPSLIPLLPVLPYRHLFPLLLSFVPLDPLRHLHRHLLGHLPSTPLADAALCAYSRLRLPHLAAQLLHSLRRRIRVRPSLQAANAVLSALARSPSTSPQASLDAFRSLIALRLHPNHYTFNLLVHTHCSKGTLADALATLSTMQGFGLSPDAVTYNTLLHAHCRKGMLGEARTLLARMKKEGITPTRPTYNTLVSAYAKLGWIKQATKVLEAMTANGLEPDLWTYNVLIAGLCQAEKVDEAFRLKDEMERLDTLLPDVVTYNTLADACFKWRRSSDALRLLEEMREKGLKPTLFTHNIIIKGLCKDGELEEALGCLKKMSDEGLAPDVITYNTLIDAYCKAGNVAKAFALMDEMVGRGLKLDTFTLNTVLYNLCKQKRYEEAQGLLQSPSQRGFVPDEVSYGTVMAAYFKEYNPEPALRLWDEMIQRKLTPSISTYNTLIKGLGRMGRLKEAIDKLNELMEKGLVPDDTTYNIIIHAYCKEGDLENAFQFHNKMVENSFKPDVVTCNTLMNGLCLQGKLDKAMKLFDSWVEKGKKVDVITYNTLIHAMCKDGDVDAALQFFADMEVRGLQPDAFTYNVVLSALSEAGRPEEAQSMLHKLTESGKLSQSFSSPLLKLSSVDETESAKDHKGNTEEETGGNPQDSALEADTKLLNELCTGGQFKEAKAILDEMMQKGMSVDSSTYITLMEGLIKRQKRQTHAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG10</accession>
    <name>A0A8I6XG10_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG10"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0157660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0157660.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0157660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0190310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="80" mass="9121" checksum="11FB086C440DB37B" modified="2022-05-25" version="1">MSTARESGRKEGLREVSMRTRWKAKNLSSSSNTRRGFVLSSCVATHAYCSLVFIPLPFGSHNIKMIHNIKMIYFKFIHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG65</accession>
    <name>A0A8I6XG65_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979913.1">
        <property type="nucleotide sequence ID" value="XM_045123978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG65"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0411840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0411840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0411840"/>
    </dbReference>
    <dbReference type="GeneID" id="123447380"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0343180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0343180.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0343180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0411840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0411840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0411840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447380"/>
    <dbReference type="OrthoDB" id="1915958at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045320">
        <property type="entry name" value="JAGGED/SL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730:SF108">
        <property type="entry name" value="PROTEIN LATE FLOWERING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730">
        <property type="entry name" value="ZINC FINGER PROTEIN JAGGED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0411840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="24800" checksum="41CD58BCCA4E0623" modified="2022-05-25" version="1">MHSPHYCSTSLFSHLRGFRSGGIPIENPYIGTPLLPVLGAAATLAYTILYTSMERASSTWFHAQAAAAAEADLLLASTPVGRRLDRYDEEAQTVCVDGKKVRLFQCLFCDKTFLKSQALGGHQNAHRKDRLAGFLGDPYSNDTPLGGAATGPSRDSAAGWSMCTYITSHGGGAAAAPATDDCRLERWGGGRAPRFVEPAPVMDPSTGRDGVVGWSRASIPSSGGGEALDLELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGJ0</accession>
    <name>A0A8I6XGJ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123427706</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044967750.1">
        <property type="nucleotide sequence ID" value="XM_045111815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGJ0"/>
    <dbReference type="SMR" id="A0A8I6XGJ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0193060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0193060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0193060"/>
    </dbReference>
    <dbReference type="GeneID" id="123427706"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0160030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0160030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0160030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0193060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0193060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0193060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427706"/>
    <dbReference type="OMA" id="ATNCCTR"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000589">
        <property type="entry name" value="RING/U-box superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF46">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="29"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="131"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0193060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="374" mass="40523" checksum="1E4D531BE7C1D9BF" modified="2022-05-25" version="1">MDEHMGRRTVGGLLFTKGGSILLFREDSSRRKAGACCPRNGCNGTRHSTDKGRPTPSHREAAATAAKEAAPTTRRSQPLRKKPPQGSSNPAEPCSETDNRTGETAAPGAGRDLLARLKDRVNASRKRSLAREMSSSSSSSGGFSASSSGGGATRSSAVSRPTRRAASRIRKADEGENAGGARRAPRRDTGGGVGARRNSDDPVMVGQRAAREQAPTEGFISGFLARYRGSLQGGSSLQDGTEDSSGYWRFDVEGSEELENYFMLSDRHRAMRMDIDGMSYEELLALGDRIGTVNTGLSEDALYKCLKRSLYTPTAPETHLDCDRKCSICQEEYSGGEEVGNMACKHYYHITCIQHWLRQKNWCPICKSVAAKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHV7</accession>
    <name>A0A8I6XHV7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123439167</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971846.1">
        <property type="nucleotide sequence ID" value="XM_045115911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHV7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221000"/>
    </dbReference>
    <dbReference type="GeneID" id="123439167"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0183810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0183810.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0183810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0221000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439167"/>
    <dbReference type="OrthoDB" id="671240at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF255">
        <property type="entry name" value="XYLOGLUCAN GALACTOSYLTRANSFERASE GT17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="76"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="520" mass="58062" checksum="8BFA3F13414E5652" modified="2022-05-25" version="1">MKSFSRNIQRDIDAAAVADGDHVGKPHSERGVRRPSRLFFLGLLYTVLWTSLVFLHHISTSVLDLKPSAFFLRDPCAGRYVYMYDLPPRFNADLARDCRRISGSPDVCKDVRNDGFGPPLTGGGEGGRSLPDRGAYETDQYMLGIIFHARMRRHECLTADPAAAAAVYVPFYAGLDAAMHLDNADLAARDALSLDLVDWLAQRPEWRAMGGRDHFLVSGRGTWDFLRRPDAGGWGNALMTYPAIRNATFLTTEASPWHGHDFAVPFPSHFHPSSDADVAGWQDRMRRARRGLLWCFAGGPRGGDTGTVRAQIIEQCGRSSSRCSLLGRSAVTKPGHYAPGHAMRLLGSAEFCMQPRGDGYTRKSTFDAILAGCIPVFFHPVSAYLQYTWHLPRDYRSYSVFIPAGDLSRNASIEEVLLNIPPEKVAQMREQVIRLIPTVMYRHPAAMGVTFKDAVDVALERVIHRVAKRRRDAAEGREHVDGVDGGDSWKYDLLEDGEEKIGPHEFDQYLYMHVTKDLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKS3</accession>
    <name>A0A8I6XKS3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor HY5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKS3"/>
    <dbReference type="SMR" id="A0A8I6XKS3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0241370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0241370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0241370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010017">
        <property type="term" value="P:red or far-red light signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010218">
        <property type="term" value="P:response to far red light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000004">
        <property type="entry name" value="Transcription factor HY5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF4">
        <property type="entry name" value="OS01G0174000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="14089" checksum="FAF9D707508E4D0B" modified="2022-05-25" version="1">MGGGGGSASSGAGDGKQLQLAAAGGGQAPAGKKRGRAAGDKEQNRLKRLLRNRVSAQQARERKKAYMTELEVKAKDLELRNAELEQKVSTLQNENNTLRQILKNTTAHAGKKPSGGGGKGGDGVKKHHHHFGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNV5</accession>
    <name>A0A8I6XNV5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123395035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044945885.1">
        <property type="nucleotide sequence ID" value="XM_045089950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNV5"/>
    <dbReference type="SMR" id="A0A8I6XNV5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468940"/>
    </dbReference>
    <dbReference type="GeneID" id="123395035"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0388240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0388240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0388240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0468940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395035"/>
    <dbReference type="OMA" id="QMYKING"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000375">
        <property type="entry name" value="Uncharacterized protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="72"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="294" mass="31213" checksum="C4890A3E0C8F2CFE" modified="2022-05-25" version="1">MRSALSHLIRSSSSASPSRLSSPVLLKNGKAFSSDATPRDSRLVDEPFKVEEAEPVKVPPPPPSPDKLLVLGGSGFVGSHVCKEALERGFVVSSLNRSGKPSISESWADKVIWNQGNLLEPASLEDSMDGVSAVVSCVGGFGSNSQMFKLNGTANINAIRAAAEKGIKRFVYVSAADFGLVNYLLQGYYEGKRAAEAELLSKFTYGGVILRPGFIHGTRRVGSMNIPLGLVGSPMQMVLQNAKPLTRLPLVGPMLTPPVSAASVAKVAVRAATDPVFPPGIVDVYGIMRYSEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQY5</accession>
    <name>A0A8I6XQY5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414950"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549:SF164">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF247)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414950.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="458" mass="50693" checksum="1F9F6F8EFA087102" modified="2022-05-25" version="1">MNTANSVDTTTIIVEMPPAAELADELIQASQKLEAVYSNILNQIHRFPASLRSLDGDYIVPRMVAIGAYHHGLPELQDMEEIKKAAAYHFCRDSGLSPEAVYHKVLAVASDARSCYTDGELQCVTSDEDFFASIMFRDGCFLLQTILWILQQGGGDGPPLPSFSCKLNSMALLRDLFLLENQIPWVVLEALMGCRLDVFPDALREFIVHIGKFFQLHTDTDLLHQPLDLKLDGNGSYSSYSKPAHLLALLHFHHSGGSSSTDELHLLGLETLPPVVTSAVDLAQTGIKLAPSKTAAFGGMALISKGALFPELSMPPLLLSELTACWLVNMAAFESTGSVHHGATSGHYIVSSYLTLLSMLMDRVEDVHELRAKRVIHSDFTNDTTLAFFKGGLTSYLYVNDRYLDILKLIEKHKLERWVWIAIHRFIYNNLKTIVAVLSIISVLVGIFKALFSLQKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRI0</accession>
    <name>A0A8I6XRI0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123431157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044970929.1">
        <property type="nucleotide sequence ID" value="XM_045114994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRI0"/>
    <dbReference type="SMR" id="A0A8I6XRI0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208160"/>
    </dbReference>
    <dbReference type="GeneID" id="123431157"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0173070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0173070.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0173070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0208160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431157"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF239">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="164" mass="18823" checksum="D05F0FED329D72CF" modified="2022-05-25" version="1">MEPIRVPTRTRIPIIYQVVEDREPADGQEFLARGVRDWMQGRLPLIDQELDMLQRQLEAQQQSRHEDEDEERFWPGLVVPASRDAVLQLPETTGAPAGARLQQTECAVCLKDFEVEDRVTTMPCDHYFHQGCISEWLKVSCVCPLCRHALPAASPQARQMEVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS58</accession>
    <name>A0A8I6XS58_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123452962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044985647.1">
        <property type="nucleotide sequence ID" value="XM_045129712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS58"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421100"/>
    </dbReference>
    <dbReference type="GeneID" id="123452962"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452962"/>
    <dbReference type="OrthoDB" id="607685at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046527">
        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120:SF62">
        <property type="entry name" value="PIR2-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20235">
        <property type="entry name" value="PIR2-like_helical"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIR2-like helical" evidence="3">
        <location>
            <begin position="59"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="PIR2-like helical" evidence="3">
        <location>
            <begin position="377"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3615" evidence="2">
        <location>
            <begin position="593"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="742" mass="83095" checksum="497C1BB11D850537" modified="2022-05-25" version="1">MEPTPAAEHQESQPMARHQESQPTSSDLGERRGLFSCLFSECDDPPFNDSNLEAHLLETILHLYEEAFERLPVHDMPVEAADQLAISMRKGGLCLGLLDPVANIILNTIALLPHDFGDMAAKRRSKRLAGRRDSSSWVVPSSCRRVTWRSIAVSSCRALVRFMVDYFGCLSEEQATRYLHWAHADLALAVQLVEHDLRAAHVAPPDPASARTQAAFKYAAICGWHPAPDDLLRLHASPLPKQRLRDAAPFLNKGGRKLTVHHVITIMDLLRYQEGAAVDLQVNLLPSARELVVYCRNLKADEGNLDISNTTSSDGFHMVTIKVERHGYHFAALRSPEDYRSMMSSCLKGAAEMHRVVNSCSSDACEYTLSLRMRLYDMIHGFYLKVFAMLPSTWLDLIPHILYAGHCYGPLDPVSNIITNSILHRLVRPIPWLGSKIKVYDILDTLSMLRVEVRSLEGLIALVRASSKPRCSTQQAMEHLSCKRCDLSQESHTSQQFAAAAAAARHPYDVLGSSLAPSLIIDLRRMLATSANGRISSESLEQIMCILKETCAPMCGPLLPYAAKLSKEAKETLLEKRSHYMKMKLFIHAELAQVLKKYAFEHPLEPKYVPSVICGLVAASESLDRFSYHVNFVAASESGIADNQLFFADINAPFSHPSKPSFCCRLPLTSAGRCYYGKASARKIVYPDSSELLECDCDITDYGTRQTDGMLEPDFVLEYRRDAGFAEDLRKYCEEQKKRDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTW5</accession>
    <name>A0A8I6XTW5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC123442571</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044974585.1">
        <property type="nucleotide sequence ID" value="XM_045118650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTW5"/>
    <dbReference type="SMR" id="A0A8I6XTW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0226150"/>
    </dbReference>
    <dbReference type="GeneID" id="123442571"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0226150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0226150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442571"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000214">
        <property type="entry name" value="WAK1-OsWAK receptor-like cytoplasmic kinase (OsWAK-RLCK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000161">
        <property type="entry name" value="Wall-associated receptor kinase-like 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF2">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035219790" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="25"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="324"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="659" mass="72172" checksum="2BBE6C5B364440E3" modified="2022-05-25" version="1">MPPPLLLLLLLSASFLRLPAPGGAACSPKACGDLSITYPFWLEEGAGRPPCGSPSFELKCNGTHAFLSRSILGQYQVARVFADNSSFVAVNHNLLPNAGCPPRWFNVSLGLGLGPYTISKKNMEVLVLYNCTDQQRAPPPGFSRTRCADGSFYRLGGVYGGHSGPGIVPPACSGLAVVPVLGFPDGEDYVPSMRQGFLLEWTVPSDDCPKCEASGGQCRYANDGTGFSCSCSGGVYPDKCGNRNPARLKTIAGVVGGIGAILFMFVVCFVYFAWYKRKKRKQAIASKEFMRSGSSTTSYSKDLELDSSPHIFTFEELEVATDGFSASRELGDGGFGTVYKGKLKDGRVVAVKRLYKNNYRRVEQFLNEVDILSRLLHQNLVILYGCTSRMSRDLLLVYEFIANGTVADHLHGCRAAERGLTWPLRLNIAIETAEALAYLHAVEIIHRDVKTTNILLDNSFHVKVADFGLSRLFPLEVTHVSTVPQGTPGYVDPVYHQCYKLTDKSDVYSFGVVLVELISSKPAVDMSRSHSEINLANMALNRIQNHEVVQLVDPELGYDTDPETKRTIDRVAEVAFRCLQMERDLRPSIKEVVEILTCVRDGDCLSKSLKKKGSQKEDAHLLTVTDGVQFSPDTVIHRFHSQSTNHSGASNASGLSNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXJ9</accession>
    <name>A0A8I6XXJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="17569" checksum="F0B410A93E26D900" modified="2022-05-25" version="1">MICFFAMEWHLPHRVSKQFGVQQRTPPDYVETSVQLHKTNRQTNKSVINWEEHHMIWVDMWNAQRHARVENDQTPDTDEAAYLRNLEWMRTEYRVIHKAAWTRSDCLDLLPSEAVDVPFNNSIREIVGAHLDYGPLHDRVVRLFFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y064</accession>
    <name>A0A8I6Y064_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Membrane</location>
            <topology evidence="2 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948528.1">
        <property type="nucleotide sequence ID" value="XM_045092593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y064"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0469030.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0469030.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0469030"/>
    </dbReference>
    <dbReference type="GeneID" id="123398085"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0388300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0388300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0388300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0469030.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0469030.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0469030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398085"/>
    <dbReference type="OMA" id="IVMILHA"/>
    <dbReference type="OrthoDB" id="779054at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF1">
        <property type="entry name" value="PRA1 FAMILY PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0469030.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="244" mass="25986" checksum="900529BE83AE8588" modified="2022-05-25" version="1">MSSSSFKPNPLSLSVPDPALDRWLRDSGYLDLLDSSAPAPAAAAPTRASPASTSSGPGAAAEVLAFARTLASLLSLNPFACLSAADLAAPTPSWSLAFIGPPGAASYSWPPTPTQARLRVQENVRRYARNYAALSILVFACCLYRMPVSLLGLLASLAVWEAVRYCRDRWGLAERAPGIGQALLHCAQIATAILLYVCNLQFALVYAVGLSYAVMMLHASLRKLTPSSLSVPANKNRRAQPRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2E4</accession>
    <name>A0A8I6Y2E4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2E4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0460510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0460510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0460510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0554400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0554400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0554400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46327">
        <property type="entry name" value="F16F4.11 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46327:SF17">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0554400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="181" mass="20689" checksum="4FB22DDA3CF34981" modified="2022-05-25" version="1">MFPVSGAGVKRSAPSDQHERPSRRSKGASSHQANKSTGKAKVVANAQDHGSDGANERRRRRHLTIDINKVPHHDDGDLDNPVPATASGVRCSLPQQHLHRALSLDSKMRELEMKKREIEIEDAILELELERVEWAAENLREDMELKTMRLENEGMRLENNRLQVEIKRKELELRVVRSKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2T1</accession>
    <name>A0A8I6Y2T1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123402967</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARR family. Type-A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952876.1">
        <property type="nucleotide sequence ID" value="XM_045096941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2T1"/>
    <dbReference type="SMR" id="A0A8I6Y2T1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596950"/>
    </dbReference>
    <dbReference type="GeneID" id="123402967"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0494700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0494700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0494700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0596950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402967"/>
    <dbReference type="OMA" id="YIAAMVE"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000159">
        <property type="entry name" value="Two-component response regulator ORR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF74">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="25"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="15302" checksum="664811D01DF60030" modified="2022-05-25" version="1">MSSVAGGVVGTTAVLPGAGGGAPLHVLAVDDSSVDRAVISGILRSSRFRVTAVDSGKRALELLGSEPNVSMIITDYWMPEMTGYELLKKVKESSTLKQIPVVIMSSENVPTRISRCLEEGAEDFLMKPVRPSDVSRVFNRVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3Z1</accession>
    <name>A0A8I6Y3Z1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123440558</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>jasmonate + 2-oxoglutarate + O2 = (1R,2R)-12-hydroxyjasmonate + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:67144"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58431"/>
            <dbReference type="ChEBI" id="CHEBI:132022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:67145"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973057.1">
        <property type="nucleotide sequence ID" value="XM_045117122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3Z1"/>
    <dbReference type="SMR" id="A0A8I6Y3Z1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303730"/>
    </dbReference>
    <dbReference type="GeneID" id="123440558"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0253490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0253490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0303730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440558"/>
    <dbReference type="OMA" id="PAFWHGD"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120091">
        <property type="term" value="F:jasmonic acid hydrolase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900366">
        <property type="term" value="P:negative regulation of defense response to insect"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000008">
        <property type="entry name" value="Jasmonate-regulated gene 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="217"/>
            <end position="319"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="376" mass="41585" checksum="947A9F1646AF5DE0" modified="2022-05-25" version="1">MADCMQEWPEPIVRVQAVAESGLAAIPGCYVKPPHDRPAQQHLAAVAVLHEPSDTSIPVIDLGELLAADEGRIDGLITEAVAAACRDWGFFQVVNHGVAPELMRAVREAWRGFFRLPISAKQQYANQPRTYEGYGSRVGVQKGGPLDWGDYYFLHLAPEAAKSPDKYWPTNPAICKELSEEYGREVTRLCELLMKVLSASLGLEETRFREAFGGAECGTCLRANYYPRCPQPDLTLGLSAHSDPGVLTVLLADEHVRGLQVRRADGEWVTVQPVRHDAFIVNVGDQIQILSNSVYKSVEHRVIVNAKEERISLALFYNPRGDVPIAPALELVTPDRPSLYPPMTFDEYRVYIRKNGPRGKAQLEGFKGKAAPAGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4P7</accession>
    <name>A0A8I6Y4P7_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">Folate receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123402893</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0590430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0489440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0489440.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0590430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0590430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0590430"/>
    </dbReference>
    <dbReference type="OMA" id="EANPECL"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="entry name" value="Folate-binding_rcpt-like"/>
    </dbReference>
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        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37390:SF1">
        <property type="entry name" value="FOLATE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37390">
        <property type="entry name" value="OS02G0592500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035312683" description="Folate receptor-like domain-containing protein" evidence="4">
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            <begin position="28"/>
            <end position="289"/>
        </location>
    </feature>
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        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Folate receptor-like" evidence="5">
        <location>
            <begin position="58"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0590430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="289" mass="31526" checksum="06BACB41E1B025E1" modified="2022-05-25" version="1">MSPPPPLRPTPTYSLLLLLFFSLTASAAQPKGVCVSPGGRFPAFSSEGKPPGKAAKGRRDLALCRIFRQNTCCDVTQTFPALLSVRKLSSTGEGSGECLHLWELLECSVCDPRVGVRPGPPVICASFCDMVFKACSEAYFSIDMKTQALSPCGLGDILCGKAHKWVSNGTELCRLAGFSVQVSDASLSEVDETFCYGGKASLDSISDTWTSSKDRPALHGMPSWDLEDFQRWAKEMPVGERISWAVGGMVLTAGLIFISKREGYSRRRKQASVAQTVRKKWNRELTRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y523</accession>
    <name>A0A8I6Y523_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123404256</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044954110.1">
        <property type="nucleotide sequence ID" value="XM_045098175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y523"/>
    <dbReference type="SMR" id="A0A8I6Y523"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611340"/>
    </dbReference>
    <dbReference type="GeneID" id="123404256"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0506940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0506940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0506940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0611340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404256"/>
    <dbReference type="OMA" id="NQKISHW"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF514">
        <property type="entry name" value="OS07G0598600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="38"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="448" mass="47850" checksum="985811414C057881" modified="2022-05-25" version="1">MVMPMASAGDGQDTEARRPLLARRHKESEDGLDDGGDASFSGAVFNLSTTIVGAGIMALPATMRVLGLVPGLTLVVLAAVLTDASIELLMRFSNAVGAASYGEAMGDSFGALGRGLLQLCVVVNNIGVMVVYMIIIGDVLSGTSSSGKHHHGVFEGWFGPNRWNGRFAILLITTLAVFAPLTCFKRVDSLKYTSALSVALAVVFVVITAGIAMIKLTRGQIPMPMLFPDVHGTWASIFKLFTAAPVLVTAFICHYNVHPIHNELKDPAQIRPIVRASLVLCSTVYVTTSFFGFLLFGEETLDDVLANFDSDLGIPYGGVFNDAVRVSYALHLMLVFPIVFHALRLNMDGLLFPSARPLACDNRRFAVLTAALLAVIFLAANFIPNIWDAFQFTGATAAVSIAYIFPAGMALRDRHGIAKKGDKVLALFMIVIATVSNGVAVYSDASSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6M8</accession>
    <name>A0A8I6Y6M8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6Y6M8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0618010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0618010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0618010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0618010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0618010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0618010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="3-5 EXONUCLEASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0618010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25136" checksum="60F1C67AF0C7852C" modified="2022-05-25" version="1">MPTIFREVVAHGATKLRVVYTNLSTEVLFFLQQLKERWFNQAPDHEKFFGLDLEYTADQRGVAVIQICFASHVLIFQWAKSDKHCPELMEFLRSGVTFASVDIRNDRLKMRHSFGIEIPAGCHVDLQTILRPRHDRTSMAHMAVALIDESYGDMKTSFPKYQHKLWEKGPLNDINIQYAAKDAYVSYELYRKIRVVNYGQRHLEELGHSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8P0</accession>
    <name>A0A8I6Y8P0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamine synthetase</fullName>
            <ecNumber evidence="3 9">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123449274</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_044982361.1">
        <property type="nucleotide sequence ID" value="XM_045126426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y8P0"/>
    <dbReference type="SMR" id="A0A8I6Y8P0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395540"/>
    </dbReference>
    <dbReference type="GeneID" id="123449274"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0395540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449274"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF109">
        <property type="entry name" value="GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="106"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="354" mass="38775" checksum="9BEBBF18621C5C29" modified="2022-05-25" version="1">MASLADLVNLNLSDCTDKVIVEYLWVGGSGIDIRSKARTVNGPITDASQLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGDNILVMCDCYTPQGVPIPTNKRHNAAKIFNSAKVAAEETWYGIEQEYTLLQKDVNWPLGWPIGGYPGPQGPYYCAAGADKAFGRDIVDAHYKACLYAGINISGINGEVMPGQWEFQVGPSVGIAASDQLWVARYILERITEVAGVVLSLDPKPIPGDWNGAGAHTNYSTKSMRQAGGYEVIKKAIEKLGKRHMQHIAAYGEGNERRLTGHHETADINTFKWGVADRGASIRVGRDTEKDGKGYFEDRRPASNMDPYVVTSMIAETTLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAF6</accession>
    <name>A0A8I6YAF6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123410276</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044959120.1">
        <property type="nucleotide sequence ID" value="XM_045103185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAF6"/>
    <dbReference type="SMR" id="A0A8I6YAF6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0642640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0642640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0642640"/>
    </dbReference>
    <dbReference type="GeneID" id="123410276"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0533930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0533930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0533930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0642640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0642640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0642640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410276"/>
    <dbReference type="OMA" id="LIMHVIR"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1396">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0642640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="489" mass="54285" checksum="A9D6518101EF7FA0" modified="2022-05-25" version="1">MGSLAPVERPHAVMIPYPAQGHITPMLKLAKLLHTRGFHITFVNTEFNHRRLMRSQPQGAGTLHGLPAFRFAAIADGLPPSDREATQDTAALSYSTMTTCLPRLKELIIKLNEEAGTSGGALPPVTCLVADGTMCFALVAARELGLRCATFWAPSACGFMGYCYYKGLIERGLFPLKEEAQLTNGYLDTIINWIPSMPKDLRLRDLPSFVRSTDPDDIMFNFFIHETTAMSQASAVIINTFDELDAPLLDAMSKFLPPVYTVGPLHLTVRNNVPEDSPLIGIGSNLWKEQDGPLRWLDNQPPGSVVYVNFGSITVMSNEHLLEFAWGLANSGYTFLWNVRPDLVKGHDNAVLPPEFSAATEDRSMLSTWCPQEKVLEHEAIGVFLTHSGWNSSLEGICGGVPMVCWPFFTEQQTNCRYKCTEWGIGMEIGEEVTRTEVEAILREAMEGGKGQEMRRRVLKLQENAVASARHDGRSMRNVDRLIQEVLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFV8</accession>
    <name>A0A8I6YFV8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="1">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123408998</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFV8"/>
    <dbReference type="SMR" id="A0A8I6YFV8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0703920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF486">
        <property type="entry name" value="OS12G0427000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="327" mass="36250" checksum="62B70B7D69FAA717" modified="2022-05-25" version="1">MAAFAGKKEAAWPLTMARYERLEKLAEGINSEVFKAWDTEDNLIVAVKRLSGSGDDGFIISTLPEVMREAMCLGACSGIPSILQRRATCVAACQREANDFFIVMDYVGRLNLRGYMQRRVCRRRPFSEDEVRRIMKQLVEGVTAVHAVGVLHLDIKPENALLDDGTEDTKEKPKKGPVEDDVRGELKEDRIVYKIGGFGMSMKGRSKRQPEVTILTPYSAPELLLHSCEYDDRVDTWGLGCIMADLLSDTGVCTFDGESDIKIMAKVFGIVGTEGTKDWSGYSGVASGQKSKLPGGRGISRLRHKFPSRKLSTAGFEVRFRGQKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGI7</accession>
    <name>A0A8I6YGI7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123395853</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044946821.1">
        <property type="nucleotide sequence ID" value="XM_045090886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGI7"/>
    <dbReference type="SMR" id="A0A8I6YGI7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0477980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0477980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0477980"/>
    </dbReference>
    <dbReference type="GeneID" id="123395853"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0396010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0396010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0396010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0477980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0477980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0477980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395853"/>
    <dbReference type="OrthoDB" id="10253115at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44378">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 17, PEROXISOMAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44378:SF2">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 17, PEROXISOMAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="120"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="185"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0477980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="722" mass="77699" checksum="D10F3353FD59A35A" modified="2022-05-25" version="1">MAATANKPLAAITADDLAAAGATEPAALHSAVRGAVDAASARGPAAVWGELSRGVLRPGVPFAVHRMLYYGCYAGSPSATPPAWTPDPDEAALTNVGRVLEARGSEIIGQAYKDPVTSFRDFHKFSNENPEAYWKMVFEEMGITFSVEPSCILRDSDVDPGGEWLPGAVLNAAANCLTAKPGRTSSDVAIVWRDEGKDSEPLNFVTVEELRKKVSLVANALDALNLVKGSAIAIDMPMNVNAVVIYLAIVLAGYIVVSIADSFAAPAISMRLKISEAKAIFTQDCILRDDKELPLYSRIVEAKAPMAIVIPARGTSTSVKGLRTDDLSWEDFLGRADHTKANIYTTVEQPAYQFSNILFSSGTTGEPKAIPWTHLTPLKAAADGWCHMDIRKGDVVAWPTNLGWMMGPWLVYASLLNGASMALYNGSPNSSGFAKFVQDAKVTMLGVVPSIVRTWKSTDCTAGFDWSTIRCFSSTGEASSVDDYLWLMGRACYKPVIEYCGGTEIGGGFITGSLLQPQALSAFSTPAMGCNLFILDSIGNPLPQDSAGIGELALDPTLFGSSTTLLNADHHEVYFSGMPEWNGKVLRRHGDEFERTTEGYYRAHGRADDTMNLGGIKVSSIEIERICNRVNDAILETAAIGVPPVGGGPEQLTIAVVFKDQSPQAEDLNQLKLMFNSALKKLNPLFKVSSVVVVSSLPRTASNKVMRRVLRKEFTQAKHSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIE5</accession>
    <name>A0A8I6YIE5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Domain X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123411510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960405.1">
        <property type="nucleotide sequence ID" value="XM_045104470.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960406.1">
        <property type="nucleotide sequence ID" value="XM_045104471.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960407.1">
        <property type="nucleotide sequence ID" value="XM_045104472.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960408.1">
        <property type="nucleotide sequence ID" value="XM_045104473.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960409.1">
        <property type="nucleotide sequence ID" value="XM_045104474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIE5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0709820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0709820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0709820"/>
    </dbReference>
    <dbReference type="GeneID" id="123411510"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0588750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0588750.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0588750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0709820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0709820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0709820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411510"/>
    <dbReference type="OrthoDB" id="596361at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000374">
        <property type="term" value="P:Group III intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090615">
        <property type="term" value="P:mitochondrial mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006521">
        <property type="term" value="P:regulation of amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001006">
        <property type="term" value="P:regulation of cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010896">
        <property type="term" value="P:regulation of triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009845">
        <property type="term" value="P:seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01651">
        <property type="entry name" value="RT_G2_intron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024937">
        <property type="entry name" value="Domain_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051083">
        <property type="entry name" value="GrpII_Intron_Splice-Mob/Def"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34047:SF2">
        <property type="entry name" value="NUCLEAR INTRON MATURASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34047">
        <property type="entry name" value="NUCLEAR INTRON MATURASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01348">
        <property type="entry name" value="Intron_maturas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain X" evidence="2">
        <location>
            <begin position="518"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0709820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="706" mass="80481" checksum="778D3A92FD672767" modified="2022-05-25" version="1">MPPPPPPRAALRRLLPATGIQPIRRRPAPPPPADQSPLPDPYALLVHDPIDLLSSLWRRAFAHPPSTPFPNLSGYASRLDLWLLSYQRACAHATGSFPPRQAVPLPTLHSLLRLRAAALRRHPAFPWGASTHLLLRSPADTPSTVPVSRRKLTARLANAPPPFQDHVVQELLLLLLEPVFEPRFSPKSHAFRPGRGPHTAIRSVRSGFAAYLWFITADLTTVVDALSSDTILSCVEKAVSDRKVLSLLKSALNAPVRPGSVPPREKDLDGLSKKRLKRKVLRKSRKKKVLNEDEPKPDPYWLRLFFGFAPEQACHVPEYGHCGMISPLLANVCLNELDWWMEERINEYFRPSRHDSIWKEAGDEGCHNPSWPEFVPSSGREKTRKMDFLRFGSHVLIGVRGPREDAVEIRRQLMEFCERTFGLRPENSSVEIEHITRGIEFLDHVIARRVIYPTLRYTASGGNIVSEKGIGTLLSVTASLQRCIRHFRKLELVKGDRDPEPLPCSPMLYSGQAHTNSQMNKFLETMADWYKYADNRKKIVGFCAYVIRSSLAKLYAARYRLKSRAKVYKIASRDLSRPLRESTNNDAPEYSDLLRMGVVDIIEGVQFARMSSIPSCDYTTFPRNWVPHHELVLHEYIKLQDPKFFCELHKTIKRREISSPQDDVSKMVWHYKVHGVYDNNRVSKKLNEWRGNDEAVNGETQLLLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIX3</accession>
    <name>A0A8I6YIX3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase APG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123411137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960002.1">
        <property type="nucleotide sequence ID" value="XM_045104067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIX3"/>
    <dbReference type="SMR" id="A0A8I6YIX3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650710"/>
    </dbReference>
    <dbReference type="GeneID" id="123411137"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411137"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF16">
        <property type="entry name" value="GDSL ESTERASE_LIPASE APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035245846" description="GDSL esterase/lipase APG" evidence="2">
        <location>
            <begin position="31"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="38768" checksum="0A43078A4F4B0C39" modified="2022-05-25" version="1">MPSSRSGSPATQWRLVLLALPLLVSCGVHGQVVPAVISFGDSTIDVGNNNYLPGAVFKANYAPYGENFRRHRATGRFSDGKIVTDITAETLGFEGYAPPYLSPLASGKNLLTGANFGSAASSYSDDTAAMYDAITLSQQLRYYKEYRSKLAAVAGRRQARTILAEALYVVSTGTGDFIQNYYHNASLSARYDVDRYCDLLVGIFSGFTDELYRLGARRIGVTTMPPLGCLPATIRLYGKGRSGCLPRLNRDAETFNRKLNATVGALVRRHAGLKVAVFDVYTPLRDLSERPAAQGFAEAGRTCCRTGKAGTRVYLCDPATAAGMCRNASSYVYFDGVHPSEAANLVIAESLVSAGIDLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJ64</accession>
    <name>A0A8I6YJ64_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain dehydrogenase/reductase</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123408250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044957336.1">
        <property type="nucleotide sequence ID" value="XM_045101401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJ64"/>
    <dbReference type="SMR" id="A0A8I6YJ64"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653480"/>
    </dbReference>
    <dbReference type="GeneID" id="123408250"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408250"/>
    <dbReference type="OMA" id="DGKHAEH"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000396">
        <property type="entry name" value="(+)-neomenthol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF137">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="307" mass="33397" checksum="9F13F4C75C527F90" modified="2022-05-25" version="1">MEGAISSRPNTRVAVVTGGNKGIGFEVCRQLAVDGGVTVVLTARDEARGAEAAERLRALGVTDAVFHQLDITDASSIATLVYFLDTRFGKLDILVNNAGVGGVEYPQELEANDGQFAGLDSKQRLEWMVKNVREPIDAARKAVETNYYGTKHVTQALLPLLQSSKEGRIVTVSSEYGLLRLISNEEARRDLNDIDNLTEERLDELLGKFLNDFEEGDALETHGWPTGFSAYKVAMAATNAYSRILARRHPMLRVNCAHPGYVKTDMSMGSGVLTPEEGAQRVVMVALLPDGGPTGKYFTKGEEASFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJ80</accession>
    <name>A0A8I6YJ80_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJ80"/>
    <dbReference type="SMR" id="A0A8I6YJ80"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035248692" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="132" mass="14214" checksum="D825736B0181FB5E" modified="2022-05-25" version="1">MAPARHLATATLAATAAVVALLLLAGGHGADAGADTSDCRVAQTAFGECTDYITSFEDAVVTPRCCQGLRTISDLATSREQRRELCECVRSVMVAAGNVIFHRVQMLRSGVACGFKPDFLPSAYYFYRCFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJH2</accession>
    <name>A0A8I6YJH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJH2"/>
    <dbReference type="SMR" id="A0A8I6YJH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0656210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000328">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000453">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase HSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF134">
        <property type="entry name" value="OS01G0960400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="533"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="809"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="824"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="834"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="561"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="873" mass="95637" checksum="46275D8E4F3C7DB1" modified="2022-05-25" version="1">MGRELLRLNLSGTLAPEIGQLSRMKIIDFMWNSIGGSIPKEVGNITSLELLLLNGNQLTGSLPEEIGFVPNLNRIQIDQNHISGSIPKSFANLNKTEHFHMNNNSLSGQIPPELSRLPSLVHLLLDNNNLSGYLPPELSQLPKLLIVQLDNNNFSGSSIPSSYGNITTLLKLSLRNCSLEGPALDASGIPQLGYLDISWNQLTGPIPSSKLASNITTIDLSHNRLNGSIPGSFSGLPILQRLSMENNNLDGSVPSDVWGNIDFSGNRSLILDFRNNALTNLSNPLTPPANFTILLSGNPICTSQNQLNISEYCQPTSVAVPGGFTNNNTLCPPCSTDLPHENILRSPIPCLCAIPLHVDYRLKSPGFWDFVPYEGEFQHYLSSGLSLSSYQLEVSTFMWEEGPRLKMNLKLFPNNTALFNSGEVLRLRDMFTGWLITDSDIFGPYELIDFIPGWYENILPRRTKSSLSTGAIVGIVIAAFAAAAILSSLVTHIILRRRSSQVSKRRTVKKILMKIDGVKDLTFEELSNCTSNFSDSALIGQGGYGKVYRGVLADGTGAAIKRTQQGSLQGSEEFFTEIELLSRLHHRNLVSLVGYCDEEDEQMLVYEYMPNGTLRDHLSAKAKEALSFPMRLRIALGSSRGILYLHMEADPPIYHRDIKTSNILLDLKFVAKVADFGLSRLAPLPEMEGIAPGHVSTVVKGTPGYLDPEYFLTHNLTDKSDVYSLGVVFLELLTGMQPISHGKNLVREVMAANQSGMILSVVDMRMGPCPWGCVERFAALGLRCCQDETDARPSMVEVVRELETIWQMTPQTDSVPSELESVSVDPSRTGTQPSSSSSSAGGSMMAYQYMSSSDVSGSNLLSGVVSSTNPRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLA7</accession>
    <name>A0A8I6YLA7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA repair protein XRCC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123452159</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the XRCC4-XLF family. XRCC4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984695.1">
        <property type="nucleotide sequence ID" value="XM_045128760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLA7"/>
    <dbReference type="SMR" id="A0A8I6YLA7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509420"/>
    </dbReference>
    <dbReference type="GeneID" id="123452159"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452159"/>
    <dbReference type="OrthoDB" id="8064436at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005958">
        <property type="term" value="C:DNA-dependent protein kinase-DNA ligase 4 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010165">
        <property type="term" value="P:response to X-ray"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000012">
        <property type="entry name" value="DNA repair protein XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010585">
        <property type="entry name" value="DNA_repair_prot_XRCC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053962">
        <property type="entry name" value="XRCC4_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053961">
        <property type="entry name" value="XRCC4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009089">
        <property type="entry name" value="XRCC4_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28559">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28559:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06632">
        <property type="entry name" value="XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21924">
        <property type="entry name" value="XRCC4_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58022">
        <property type="entry name" value="XRCC4, C-terminal oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50809">
        <property type="entry name" value="XRCC4, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XRCC4 N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="XRCC4 coiled-coil" evidence="9">
        <location>
            <begin position="125"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="30952" checksum="8B5D3816B7DEE748" modified="2022-05-25" version="1">MATAAAAPRHSCAKLSVAVEDPKAPGGGGIFVKATWLPTRFSLAVTDGAGAWVADASDAEVRLRAEQWDQPVSEYLALAERYLAFHQPSSTYSFHEAGAGRRLSWTFEKQGTKLEWRWKLQPAPHPQQTIADVLDFLMDANIRLSEEVVRKTQSFEKLKQEAENCLQQSDRFNNEKAEFEQASFTKFVAVLNSKKAKLRQLKDKIAALESADKAPKEEEEEEGNSTDRTEPIEEGSDKDQSVNDEPSETGSGGDTHSSPEKSAAAAATSRGRRGRKRTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YP84</accession>
    <name>A0A8I6YP84_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC123404738</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044954619.1">
        <property type="nucleotide sequence ID" value="XM_045098684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YP84"/>
    <dbReference type="SMR" id="A0A8I6YP84"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546030"/>
    </dbReference>
    <dbReference type="GeneID" id="123404738"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0453900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0453900.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0453900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0546030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404738"/>
    <dbReference type="OMA" id="WALRMRR"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000260">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF81">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE CEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035173494" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="33"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="702"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="790"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1091" mass="115144" checksum="75CF09D88F43A8AB" modified="2022-05-25" version="1">MAGRGRGGALAAAAALLLPVIVLLSWCWGGVAQPSPRCGDGDLAALRGFSTGLDAPVDGWPADDNGCCAWPGVVCGGAGVVGVVLPNRTLRGEVAASLAGLTALRVLNLSGNALRGALPPGLLRLRRLEVLDVSSNALAGALVDAAGAGLIELPAVRVFNVSYNSFNGSHPVLPSAVNLTAYDASGNAFEGHVDAAAVCTSSPGLRVLRLSMNRLSGDFPVGFGQCRFLFELSLDGNGITGVLPDDLFAATSLRYLTLHTNSISGEVPVGLRNLTGLVRLDLSFNAFTGALPEVFDALAGTLQELSAPSNVFTGGLPATLSLCVNLRVLNLRNNTLAGAIGLDFGAVNSLVYLDLGVNKFTGPIPASLPECTGMTALNLGRNLLTGEIPPSFATFPSLSFLSLTGNGFSNVTSALRILQRLPNLTSLVLTKNFRGGEAMPEDGIDGFGKIEVLVIANCELTGAIPAWLAGLRKLKVLDISWNRLAGPIPPLLGELDRLFYLDISNNSLQGEIPASLTRMPALLAGSGNGRDDDDEKVQDFPFFMRRNVSAKGRQYNQVSSFPASLVLGRNNLTGGVPAALGALARLHIVDLSWNGFSGPIPPELSGMTSLESLDVSHNALSGAIPASLTRLSFLSHFAVAYNNLSGEIPVGGQFSTFSRVDFAGNPFLCGFHVGRKCDRERDDDDQATDGSTTWSNEGRRSAASAGVVAAICVGTTLLVAVGLAVTWRTWSRRRQEDNACRVAAGDDEESLDSSAARSSTLVLLFPGDEEEGETTTVVTLDEVVKATGDFDESRIVGCGGFGMVYRATLADGRDVAVKRLSGDFHQMEREFRAEVEALSRVRHRNLVALRGYCRVGKDVRLLIYPYMENGSLDHWLHERANAGDALPWPARLRIAMGAARGLAHLHGGGGGARVMHRDVKSSNILLDAAMEARLGDFGLARLARGSDDTHVTTDLVGTLGYIPPEYGHSPAATYRGDVYSMGVVLVELVTGRRPVDMAARLGARDVTAWAARLRREGRGHEAVDAAVSGPHREEAARVLELACACVREDPKARPTAQQLVVRLDAIAGAAATQEVCSDAQHTVDDRSFGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPX4</accession>
    <name>A0A8I6YPX4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123410594</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959473.1">
        <property type="nucleotide sequence ID" value="XM_045103538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPX4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636630"/>
    </dbReference>
    <dbReference type="GeneID" id="123410594"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0636630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410594"/>
    <dbReference type="OMA" id="VKDWIID"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="12"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="190"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="39485" checksum="CAF7D4208852E7AB" modified="2022-05-25" version="1">MGGTGGGGVWPVVVMVFLNVIAAVMVSLVKVAMNGGLDPLVLVTLQQLTAACFLGPIAFFREGKARPKMTLEIFAYLFASAALGAALRQYMIFVALRYTTATFVTAFSNVAPVLTFLLAAATRSESLNLRTATGAAKLAGTLVSLAGAMVLTFYRGVPLTHAHHGGQLHYSAPSPASSVADPGASKRWTLGTVAILGNCVCLSCWFLLHGRLARKYPYVYSCNAFMSTFSFLQVAAVGLCAHRSLGLAAWLVTSKFQILTILYAGVVGCGVSFVLLTWCIEKRGPVFVAAFIPVVQIIVSVMDFTVLHETLYLGSVLGSVFVIGGLYLLLWGKRQEALQQPPKVAEDESEQQQQPQQQQQQVQVQMQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YSZ1</accession>
    <name>A0A8I6YSZ1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YSZ1"/>
    <dbReference type="SMR" id="A0A8I6YSZ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0748250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15684" checksum="DB858473D40C710E" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDVEVCEPEIPTHIKILVEKLNLVEKENKYLKKKLKIIEDKNMELELHVADVVDDRKMKIDAMRLKMDAMRLKMDEMRLKIMNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YV87</accession>
    <name>A0A8I6YV87_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YV87"/>
    <dbReference type="SMR" id="A0A8I6YV87"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0684830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0684830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0684830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0684830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0684830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0684830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF189">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0684830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="198" mass="23428" checksum="ECD865591826B84C" modified="2022-05-25" version="1">MPPRRRSASGYRGVRERPNGRFYAEIRSGNLRLSLGTYDTAHEAAHAFDATAWRLGRPPRQMNFQDVYTLQQALDVAPPPRVNSAQDRAEHTAWQRRLLVAQEDERVMAEWRRRHPEDVAYKQGYWTRRREEDTRRRRDERLDRRRRKALASAQADLVNAGGSSFFTEEDERWFDIWLSTSDDTNDDDDDADDWSDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YYL2</accession>
    <name>A0A8I6YYL2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="15">PARP</shortName>
            <ecNumber evidence="15">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123410032</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_044958845.1">
        <property type="nucleotide sequence ID" value="XM_045102910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YYL2"/>
    <dbReference type="SMR" id="A0A8I6YYL2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726670"/>
    </dbReference>
    <dbReference type="GeneID" id="123410032"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0602690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0602690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0602690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030592">
        <property type="term" value="P:DNA ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08002">
        <property type="entry name" value="WGR_PARP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000023">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000005">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.140.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.140.10">
        <property type="entry name" value="WGR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 15" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAP" evidence="17">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="17">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="17">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="20">
        <location>
            <begin position="258"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="19">
        <location>
            <begin position="383"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="18">
        <location>
            <begin position="509"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="734" mass="82104" checksum="8622D507C8FF33DA" modified="2022-05-25" version="1">MSAKLRVEELRAELQRRGLDASGTKPALVLRLDAAIRKEEKEVASAAEMCNVDGIDGLAMDGKAEGKSKRKRKRRGEGETEEENDMLDAAKLESMGYRELQALAKARGLNANGGKKDVLHRLLSSPATSVVDCGIQDKKEVAEADDGKVDELKKENIVTDVMLDAAQLQGMSYRELQALAKARGLASNGIKKDVIERLLLTPANPVAVADGGFQEKKKLAKGGVGEVEEEVKKEKIVKATKKGAAVLDEHIPDDIKMTYHVLQVGDEIYDATLNQTNVGDNNNKFYIIQALESDAGGSFMVFNRWGRVGARGQQKIHPCSTRDEAIDEFEGKFEDKTKNSWSNRKNFECYAKKYTWLEMDYGEVDKETTQVQKKGSITDQIKVTKLETRTAQFISLICNISMMKQQMMEIGYNADKLPLGKLSKSTILKGYDVLKRISIVISRADRRQLEQLTGEFYTVIPHDFGFKKMREFLIDTPQKLKAKLEMVEALGEIEIATKLLEDDSSDQDDPLYARYQQLHCDFTPLEVHSKEYSMIKTYLTNTHGKTHSGYTIDILQMFKVSRHGETERFQKFASAGNRMLLWHGSRLSNWAGIFSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCYASQTSRSGVLLLCEVALGDMNELLDAKYDANNLPKGKLSTKGVGQMAPAESKVTEDGVVVPLGKPKEEPSKRGSLLYNEYIVYNVEQIRMRYVLHVSFNFKGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z4L5</accession>
    <name>A0A8I6Z4L5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7597 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z4L5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0626670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0626670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0626670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0626670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0626670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0626670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056018">
        <property type="entry name" value="DUF7597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075:SF10">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075">
        <property type="entry name" value="OS02G0499800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24530">
        <property type="entry name" value="DUF7597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7597" evidence="2">
        <location>
            <begin position="8"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0626670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="442" mass="47810" checksum="0CF513ACB53DBE5C" modified="2022-05-25" version="1">MVNFELYPEFFLPPGHNIIDGGPDRLPRTYTTPAVPITRRHERFVIADVHPAPSADHLVRGIGFRGQEGFRHGCLMLLGVPLDFRNTEDLRAAVNTFREFHHWVNDDPYLVSWSAPVYILGAAPAEVLPNDEDPMPLNGNPHPLPGQLVNDNLQFSLPPYPALGWNAMLLAPEDPVVPAAADDGGWGQPPTAAYGGGWEPEAALEAPVADPVQDQESMDPAAEPEEDVLGVEEAQDDNPLAIVPYKPPQIQSGNLFVGAAMVFYGPPLPPVLSWSRTFDALMGAATSMHVPRQLQMPMVAPVVIPKRSWAAAFDSAAPALPPPEPAEDFSFQSPAPTKKGTRKKATPVVDSSVRRCTRGSIKRDGYKQILHELPAHVPKKRKPKAKPMPSPAQESDDVQVSPATPIPVIQAVGQTLGIAPEKLTVDRLMEDPADSAPSSADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z5Q2</accession>
    <name>A0A8I6Z5Q2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5Q2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33978">
        <property type="entry name" value="SERINE/THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33978:SF4">
        <property type="entry name" value="SERINE_THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="175" mass="18700" checksum="E961326DD0A83FDE" modified="2022-05-25" version="1">MARHIQADHESADDIGGGAGAALVLWDCGSALYDSYELTSFRRQLDATVLACRSLSMPHLPAASQAVHPAGRRKRARRLPALLRKLFSKVLRLASARGARHDRYRAYEGYGHGGYSGTGSPWSGALTSIPEESGGSPEARSSPVVPGPSALRRAQSERFIGGKTASPMVQFDVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z8X3</accession>
    <name>A0A8I6Z8X3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear condensin complex subunit 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123407292</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND3 (condensin subunit 3) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956333.1">
        <property type="nucleotide sequence ID" value="XM_045100398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z8X3"/>
    <dbReference type="SMR" id="A0A8I6Z8X3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669710"/>
    </dbReference>
    <dbReference type="GeneID" id="123407292"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0555710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0555710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0555710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0669710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407292"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025977">
        <property type="entry name" value="Cnd3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF5">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12719">
        <property type="entry name" value="Cnd3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear condensin complex subunit 3 C-terminal" evidence="9">
        <location>
            <begin position="521"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="939"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="972"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="984"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1026"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1054" mass="114665" checksum="ACFC3F6A1A29CB11" modified="2022-05-25" version="1">MSPAAAVSSDDETRRLAREAARVFDECRASLAVHPRKLRELAAVRASASGGRFLPAFCNAITPLFEVPRRSPASDRVTRFAVAFASSSASAGADGTGFLEGFLRFLLVASAAALRPARFRACQIISEIIMQVPDDAEVSSEIWDEVIDGMKIRVQDKIPAIRVFAVRALSRFAIDEEDGGIIGIFLETLEKEQNAEVRKTIILSLPPSSATLESIIESTIDTSESVRRAAYFVLSTKFPLQSLSIKQRTTLLHRGISDRSPSVNNECLRMLKEEWLVKHCGGDVIALLRFLDVETYESVGESVMGVLLKDGAVRVQDGQTIMQYFTENTEDAEQVSSIKLMEAEVALYWKVMCMHLQAEAQIKGSEAATTTGTEAAVYASEASDKNDLLDGVLPSTVAEFVDLVKAHLSAGPNYHFASRQLLLLGGMLDFSDSMNRKVASSFLYELLNRPLEHEVDEDGNQIAIGDGVSLGGDLQWAKAVSELAKKVHASVGEFEMVIATVVEELARPCRERTADFMQWMHCLAVTGLLLENTSTLRSLEGTAIRPSEVLQSLLLPAAKQKHVDVQRAALRCLCLFGLLENKPSTELVKQLRLSFINGPDLVSAMASKALIDLVTWHGPQELDQAIGTEPSDANYEKTQFAPVDISDLNDDDLNVGILSILFSGFHKDDWGFSPEGDNHDNVLTILGEGFAKILLLSENYPSISADLHPVILAQLVRLYFLEESKELGRLKQCLSVFFLHYPSLSDKHKRCVSSAFVPLMRAMWPGIHGNPGGSGPVVSKRRKHAVQAARFMVQMVQSPLFSAETAEQACMSPESLSGSPGLFVNYDISEEGLAIRIAVEVGNSQDKKSAPVRAYALALCKVAVLLRFRQSEQKAIKCMRGLVNTLAASAASDKDLMKELAQMASRLKSLDESPEEELPQDQADALFEQLGLADGIKLDTVNPVVPPTPAPSRSGRAPPARRRARRAASSSDDSEAEAEAESLDVTSGSQVQATPSVTTARAQRASKTAALSKFKSSVKPSLEAASDSEQEEMSGLTTDEDPSDDSDDPDEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z9F3</accession>
    <name>A0A8I6Z9F3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(adenine(34)) deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123411743</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine(34) in tRNA + H2O + H(+) = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="SMR" id="A0A8I6Z9F3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0676570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676570"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000005">
        <property type="entry name" value="tRNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF179">
        <property type="entry name" value="TRNA(ADENINE(34)) DEAMINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="11">
        <location>
            <begin position="1391"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="257"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="400"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="775"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="861"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="957"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1004"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1103"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1207"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1355"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="281"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="868"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="962"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1042"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1063"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1115"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1147"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1207"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1224"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1594" mass="178096" checksum="C8C0377BD668E782" modified="2022-05-25" version="1">MYSSYSAAAFALRAAKPAYNYNAHSPYAPSHHCSQQHYDGDGAHRELLPLNPHLSPRFLLDGYLLRHSTHLLLLSARLRPPLPTPPPPHSCCHRRAAARCCCSGGGGGVRSYVQHVTWGLEGRGHRCCGQGAGRSDLGVGCRRLKARGCGCGSYGSGRLRLGTGCGHRDTPRLLGRAVRQEVWEYEGGQWPHRSCSMECHSDWDDEEDEDECGHAQWELPGKASFRRKWKEEEDDICRDCRERKGVENEYYGEAEYSGRRRENRDVNGAHDRHRYHRRRLEQRGYIDGDDTRRRSDVMEGRDRREFESDEARNVGARRYSEDDRKHDQRRERRDSEYEDVLDARRVGAGRYNEDDRRFDRRTGRMDFEIDSGDDVRRDGRRLRNDDARFVIRNTRRKEYMEEDDQDMAERKHYSRGRSASAFHEDDSQRASSSRNTVDTRVARGNSSSRVRWDDNVDRRAAQTLEDREGRYSSLVGLSNDEKAEYNYDDARIFRVRDSRTGTQDVKVITEDDTRLSSGSKNSSIMKHHSDIDRKVAARKDETKKSSLKVAESLSEFRGNNTELDSATRINQLEDRRNYIDNKSSSLQSSVKMASDSRRQIDQHDVVDQQVVALTDSRTSTEKLTDIKMDSSQHVSRASHSQRNYDEVNQMDIDDRSTSVENITHITRDKRRYINQQVIHETDVDVQNVTHVDVSTIHASDISASRSSQNHSETRSDVNSISNISLIDRARGQQEQIHQNKVIACDNTIVRGSQSHHDTGVYGQVHSASVIDSTKEMQEQAELTKAHTSNAATTSTSESHIQTRIYDRFQPSSSVNTVSSVKEQIDLTKIHSSDTAGVSNSHNRSNNEACRTSVLNIVEARDSRDESELQITQGSGIDRSNQVGATFSESSQDSRERLTRVEETGRLVQHNTVLNSQHTGTSRISDDKDITSLGIESTEEASTINVDMEQRGTILGRSETTARENIQGGSSARKSVNESVLASASRLEKSSTFHVGQFVSELQRGVSDAGTTTKKTEKSIMEGTTRSSSRSRMKGPSDEIWDVQSTTSQETFKTADKEEGSSADGGTNSASQTPKSETAIAKNVHKSLWAYVADIVRLGWVKHGESHDSTSKSFKKSSSSNSQRSEGWLSSQGHDNDGIQKRNWGSKQNDHSLMKSHSGESESIVASTLKDESLPTGTQGLQISGAGNVPEVGRSKGEFVPKITKDDVQISGERAKQSEVGASPKGNTMGSSAEDSISTSVDVTVGHSPEHEAGSSSGISTQGSAEIKSGKGVSAGTSCTSIKAKEAGRSEDAGSWRYGPSVDITPYQFPQTQAMVPHENTSSAFLESTELPTGGSTGMKEKIVEHKAPEVLRTEEKDAELKRRKIQRNKQVLKETFDEWEEAYQHDAKQRKTDELFMKEALLEAQRAADIWEVPVGAVLVHNGEIIARGCNLVEDLRDSTAHAEIVCIREASSKLKTWRLADTTLYVTLEPCAMCAGAILQARVDTVVWGAPNKLLGADGSWVRLFPGDGQTSTLDPANQKQAAGPVHPFHPKIIVRRGVLSIECSEIMQQFFQLRRKKKRAESPPRAHYSGHRHHPIKFFTKMQHMFGTVFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3R3</accession>
    <name>A0A8I7B3R3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metal-nicotianamine transporter YSL7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123430734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970514.1">
        <property type="nucleotide sequence ID" value="XM_045114579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3R3"/>
    <dbReference type="SMR" id="A0A8I7B3R3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191640"/>
    </dbReference>
    <dbReference type="GeneID" id="123430734"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0158850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0158850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0158850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0191640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430734"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF48">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="605"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="642"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="695" mass="75815" checksum="FAB5DE387DD8DD32" modified="2022-05-25" version="1">MEPSTPPAATHHLWGMEDEHPSTERVFEGEPVPSRSETITVRSVAVSIVLGCTLSVVAMKLALTSGFAPSLAIPSGLLGFFLPRLWVRLMNSCEASQLPFTRQENTVIQTCVVACTSIAYSGGFGTYILGMSKDSAEGGVGGDGLNVEEPHIGRMIVFLLLTSFAGMFAIMPFRNSLIIHRRLTFPSGTATAHLINTMHTPQGAKQASQQVAMLLKTLFGTVAWSIFEWFFSGGEGCGFQAFPVFGLSAFRLGFYFNFSMTNVGVGMFCPYKITISMLAGSLISWGLIWPYIQTKEGDWYPQGLDGDNISGINGYRVFIGISMILADGLLHMLCILVRTLYTMYKQQENPQPRQSNNGQPFQCLSAVLDRTAYSFDDRRRTQVFLRDRIPTMAPVIGYMVLSLISTVVIPQLYPQLRYHHVAFAYIIAPVFAFCNAYGNGITDMNMATTYGKIAMLIFSSWVGLRDGGVVAGLAACAIIVSNVSTASDLMQDHKTGYITLTSPHTITICQAAGTALGCVVNPVIFWVFYRVYNSGAHDDSNSIGPYAKVYRGISVLGMTENGLPKHTMLLCKIFLALALSISVLREVSTHRRWRVRRYIPSTIGMAVAFFVPPTIPVGMVLGSAVIYLWGRCDRDDMRLMSPAVACGLICGDGFGSLLSSMLTLLKATPPICIMFVSRGVNESLDAFLAAKGMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4A9</accession>
    <name>A0A8I7B4A9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4A9"/>
    <dbReference type="SMR" id="A0A8I7B4A9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092940"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0076520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0076520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0076520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0076530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0076530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0076530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0092940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0092980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="21394" checksum="37A8D5D3333CBAFB" modified="2022-05-25" version="1">MAALRHAARRLCERALQQRGALQQRGAQARSSFVADEPRRLLLNGTSPGRRLMSNSSIGGKPASRAEAKGELELLEWQFKKKKEELFDLLIAVKCSDLYPQLSWKDIETHRLISHLASQVEPRPHDPIWRRFRRTRRLNEFNTICAFLVLYGQLFAYWVQTNIREYGAASKNSEANSVSEPKNDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7G4</accession>
    <name>A0A8I7B7G4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123449248</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982330.1">
        <property type="nucleotide sequence ID" value="XM_045126395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7G4"/>
    <dbReference type="SMR" id="A0A8I7B7G4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0394710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0394710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0394710"/>
    </dbReference>
    <dbReference type="GeneID" id="123449248"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0328410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0328410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0328410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0394710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0394710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0394710"/>
    </dbReference>
    <dbReference type="OMA" id="HMPAMEV"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000003">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor ANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF204">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="288"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="390"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0394710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="634" mass="68185" checksum="C6819AB48E9FB0D3" modified="2022-05-25" version="1">MRAMAGGGTNCLGFSLSPHIDMAMEVPSSSGPDHAQPASASAMSTNAATCNFLFSPPAQMAAPPPGYYYIGGAYGDGTSTAGVYYSHHPVMPVTSDGSLCIMEGMMPSSSPKLEDFLGGGNDGGHDTVTYYSHHHQQQGQQDQEGSRVYQHHQHYQQQQQELAPYNFQHLTEAEAIYQEATAPMDDAMAAAKNLLVTSYGSCYSNAGMQPLSLSMSPSSQSSSCVSAAPQQHQMAAVASAAASMAASQGGSNGGGEQCVGKKRGTGKGGQKQPVHRKSIDTFGQRTSQYRGVTRHRWTGRYEAHLWDNSCKKDGQTRKGRQVYLGGYDNEDKAARAYDLAALKYWGPSTNTNFPLETYREEVEEMKSMTRQEFIAHLRRRSSGFSRGASIYRGVTRHHQHGKWQARIGRVAGNKDLYLGTFTTQEEAAEAYDVAAIKFRGLNAVTNFDITRYDVDKIMESSSLLPGEEARKVKPIEAANHVPANAMHNGGAELSHAEEGSSGVWKMVLHGTPQQAAPCTTEVADIQKGFMDGDSRSSLHGVAGLDAESAAHEIDASGKINYSNSSSLVTSLSNSREGSPERFSLPSLYAKHPNAVSLATMSPWMAMPAPTATHALRGPNSSIPTMPVFAAWTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7P0</accession>
    <name>A0A8I7B7P0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123447213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979730.1">
        <property type="nucleotide sequence ID" value="XM_045123795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7P0"/>
    <dbReference type="SMR" id="A0A8I7B7P0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407470"/>
    </dbReference>
    <dbReference type="GeneID" id="123447213"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447213"/>
    <dbReference type="OrthoDB" id="1063785at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02043">
        <property type="entry name" value="serpinP_plants"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF316">
        <property type="entry name" value="SERPIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="51"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="434" mass="46752" checksum="0C8707CEF902EFC4" modified="2022-05-25" version="1">MQSVKEFVLRCLWVCTEAEPEPAPAPYPALAPYPALAPAPATSDGLQAFALGLNKRLADDDAGRSGNLVTSPLSVYAALSLVAAGAREGTLDELLRVLAAPSRDFLAGHVRALAEHVLADGSLTGGPRVSFACGVWHDSTTPLRPAYRVAAAESYKAVARSANFRQEPEEAREQINAWVAAATNDLIPSILSPGALSSCTVLVLANAIYFKGKWEKPFDKELTEDGKFHRLDGTAVDAPFMRGLGRHNIACHDGFKVLQLRYVQGRSSPGQPQPPPIYSMCVFLPDARDGLWGLTDKIARDPGFLRKHLPCDDVLVGDFRLPKFKVSFGVTMKGILQDMGLKEAFELGKADLSDMVEDGAGKLALHKIVHRAVIEVNEEGTEAAAATVATMIMCSYTVRPRVDFTADHPFAFFVIEEVSGATLFAGHVLDPTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BG80</accession>
    <name>A0A8I7BG80_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">High-affinity nitrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123405196</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NAR2.1. Imports nitrate with high affinity when expressed with NAR2.1 in a heterologous system (Xenopus oocytes).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044954916.1">
        <property type="nucleotide sequence ID" value="XM_045098981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BG80"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543400"/>
    </dbReference>
    <dbReference type="GeneID" id="123405196"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0543400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405196"/>
    <dbReference type="OMA" id="MANIVAC"/>
    <dbReference type="OrthoDB" id="605532at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071249">
        <property type="term" value="P:cellular response to nitrate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015706">
        <property type="term" value="P:nitrate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000048">
        <property type="entry name" value="High affinity nitrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000053">
        <property type="entry name" value="Nitrate transporter 2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
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        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054186"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="54627" checksum="9C791BA082C56BEB" modified="2022-05-25" version="1">MEVEAGAHGDTAASKFTLPVDSEHKAKSFRLFSFANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLAKADIGNAGVASVSGSIFSRLAMGAICDLLGPRYGCAFLVMLSAPTVFCMAVIDDASGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIGTVNGLAAGWGNMGGGATQLIMPLVFHAIQKCGATPFVAWRIAYFVPGMMHIVMGLLVLTMGQDLPDGNLASLQKKGDMAKDKFSKVLWGAVTNYRTWIFVLLYGYCMGVELTTDNVIAEYYFDHFHLDLRAAGTIAACFGMANIVARPAGGYLSDLGARYFGMRARLWNIWILQTAGGAFCIWLGRASALPASVTAMVLFSICAQAACGAIFGVAPFVSRRSLGIISGLTGAGGNVGAGLTQLLFFTSSQYSTGRGLEYMGIMIMACTLPVALVHFPQWGSMFFPASADATEEEYYASEWSEEEKAKGLHIAGQKFAENSRSERGRRNVILATSATPPNNTPQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGM6</accession>
    <name>A0A8I7BGM6_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0566100.1">
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15959" checksum="F902160AB07DBF54" modified="2022-05-25" version="1">MGMSPYKMVYAKACHLPLELEHQAYWAIKEGNYDFKLAGEKRLFDISSLDEWRTQAYENANCLRKKLKECMTKKSKTVIQCRIPSSFVQFLFQFFVGKLLSKWEGPYVIEEVYHSGALKLNNFEGKKTKSAKWEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJ85</accession>
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            <fullName evidence="2">Structural maintenance of chromosomes protein 5</fullName>
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        <lineage>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the SMC family. SMC5 subfamily.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="42"/>
            <end position="249"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
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        <location>
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            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0697740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="258" mass="28894" checksum="A1E559A527AE8394" modified="2022-05-25" version="1">MAPACAAKRPKLEPSASASTSAAQHEHQRGDDDYVLGNIMEIELCNFMTYGRLVCRPDPRLNLVVGPNGSGKSSLVCAIALALATDPYILGRASSVGAFVKRGEESGHVRLSLRSQSQASDIHITRKIDANNKSEWLLDGATVPKKHILDVIKKFNIQINNLTQFLLQDRFCEFAKLTPIQPLEDTEKAVGNPNLPVQHRQLIERSRELRNIEVAIKQKEQTLNNLKALNAEQEKDVQRVRQRDKLFKKAELMKKKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJJ4</accession>
    <name>A0A8I7BJJ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJJ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0593210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0593210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0593210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0715180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000220">
        <property type="entry name" value="Cyclin superfamily protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin C-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="8"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18931" checksum="555690C2A88E81FF" modified="2022-05-25" version="1">MTAPTAKCFLRRFVRAAQVCDEDPPLHLEFLANYVVELSLLEYSLLAYPPSLVAASAIFLMKFILQPAKHPWISAAFTDLRVFAESKCCLIKLTCTTSHCKLEAELYPCPLHTVQAVGTMRLCEGAAPPFSVGVIFLQSEKSTVHISTNLSGRSNVQLQCRQNSSGTQHASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2CRE6</accession>
    <name>F2CRE6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Receptor kinase-like protein Xa21</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LOC123412477</name>
    </gene>
    <organism evidence="29">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="29">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="30" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="30">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Receptor kinase that detects X.oryzae pv. oryzae protein Ax21 to promote innate immunity. Following X.oryzae pv. oryzae protein Ax21 detection, undergoes cleavage, releasing the processed protein kinase Xa21 chain.</text>
    </comment>
    <comment type="function">
        <text evidence="23">The processed protein kinase Xa21 chain released by protein cleavage after X.oryzae pv. oryzae protein Ax21 detection translocates into the nucleus where it can bind and regulate WRKY62, a transcription factor. Confers resistance to the bacterial pathogen X.oryzae pv. oryzae (Xoo).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AK354198">
        <property type="protein sequence ID" value="BAJ85417.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961359.1">
        <property type="nucleotide sequence ID" value="XM_045105424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CRE6"/>
    <dbReference type="SMR" id="F2CRE6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0737830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0737830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0737830"/>
    </dbReference>
    <dbReference type="GeneID" id="123412477"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0611910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0611910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0611910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0737830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0737830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0737830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412477"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000432">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF151">
        <property type="entry name" value="OS02G0216000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="15 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 27" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730775" description="Receptor kinase-like protein Xa21" evidence="27">
        <location>
            <begin position="23"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="721"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="758"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00054320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00056628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00072040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="BAJ85417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0737830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1065" mass="111997" checksum="14535F874856251C" modified="2011-05-31" version="1">MARPAAASVALLLLSCVAAAAGTDRDALLAFKAGVTSDPTGALRSWNNDTGFCRWAGVNCSPAGRVTTLDVGSRRLAGMLSPAIADLAHLELLNLTDNAFSGAIPASLGRLGRLEWLSLCDNAFTGGIPAALRGLGNLTTAYLNANNLTGRVPAWLGAMPALMKLRLSTNSLSGRIPPSLANLKTIQRLELAENQLEGDIPDGLTRLPNLQFFTVYQNRLSGEIPPGFFNMSSLQGLSLANNAFHGELPPDTGAGWPNLLYLFLGGNRLTGRIPATLSNATKLLSISLANNSFTGQVPPEIGKLCPESLQLSNNQLTATDAGGWEFLDNLTSCDALTGILLDGNKLAGALPSSVTRLSTQLMWLSMSGNRISGVIPPSINKLVGLQALDLRHNLFAGTIPEGIGKLENLQELQLQGNELTGPVPSTIGDLTQLLSLDLSGNSLNGSIPPSLGNLQRLVLLNLSGNGLTGVVPRELFGLSTMSSAMDLSRNQLDGVLPREVGQLAKLTFMALSGNRFIGDVPAELGGCQSLEFLDLHSNLFAGSIPPSLSRLKGLRMMNLSSNRLSGAIPPELAQITALQGLDLSRNELSGGVPAGLANMSSLVQLDVSGNNLVGDVPHRGVFANATGFKMAGNSALCGGAPQLRLQPCRTLADSTGGSHLFLKIALPIIGAALCIAVLFTVLLWRRKRKSRTTSMTARSVLNGNYYPRVSYADLAKATDGFAEANLVGAGKYGCVYRGTLALKTKGNLSHEAMAVAVKVFDLRQAGACKTFLSECDTLRNARHRNLIGIVTCCASVDAAGGEFRALVFDFMPNSSLDRWLHPGPSDVRKHGGLSLVQRLGIAVDIADALSYLHNSCDPPIVHCDLKPGNVLLGDDMTARIGDFGLAQLLLLDAPGGTESTIGIRGTIGYVAPEYGTTGSVSTAGDAYSYGVTLLEILAGKAPTDGGLGDGTTLPELVAAAFPERIEQVLDPALLPMEELDRSVSVSASISTMSTASLSYSEDSEVRVTARDCVVAAVRVALSCCRRAPYERMGMREAAAEMHLIRDACLRACGAEKPVVQDLPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2CRY9</accession>
    <name>F2CRY9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123452797</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK354391">
        <property type="protein sequence ID" value="BAJ85610.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK358288">
        <property type="protein sequence ID" value="BAJ89502.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK364252">
        <property type="protein sequence ID" value="BAJ95455.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985449.1">
        <property type="nucleotide sequence ID" value="XM_045129514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CRY9"/>
    <dbReference type="SMR" id="F2CRY9"/>
    <dbReference type="PaxDb" id="4513-MLOC_54622.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520940"/>
    </dbReference>
    <dbReference type="GeneID" id="123452797"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433110.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0520940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452797"/>
    <dbReference type="OMA" id="MRSAWSH"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CRY9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="265"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="483"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ85610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ95455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="508" mass="56309" checksum="D3535AD801FFA726" modified="2011-05-31" version="1">MRGMDGSSGVWGGLNSGVVLSLIAVLWTVVWQNLQHLQLQHFFKRHLGRHARRLAALVDPYLSVTIAEYDGGRMRRAEAYEEVKAYLAASTSRSARHLRAEGARDADRLVLSMVDGEEVADALLPEEGGGAVFWWAYSRPPPQQDRRWGGGFGGGGGDEENRRFYRLFFLDRHRDQVLNAYLPRVRRQGRAVMVQNRRRKLFTNISTHQFTDGGYTRSAWTHVPFEHPKTFATLAMDPAAKKEVMDDLDAFKAGKQWYERVGKAWKRGYLLHGPPGTGKSAMIAAMANHLDYDVYDIELTSVHSNTDLRKLFIGTTSKSIIVIEDIDCSLDLTGARNAKKKDAAPEDDDKGKGDKKGATDATSKVTLSGLLNFIDGLWSACGGERVIVFTTNHLEKLDPALIRRGRMDKHIEMSYCRAPAFEFLAKAYLGVEEHELFGAVGALLREVDMTPADVAENLTPKSADDDADSCLRGLVAALEKAREVKASSGGQEKQPEEEDGGVVAVDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSW9</accession>
    <name>F2CSW9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401881</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK354721">
        <property type="protein sequence ID" value="BAJ85940.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951672.1">
        <property type="nucleotide sequence ID" value="XM_045095737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CSW9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0567190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0567190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0567190"/>
    </dbReference>
    <dbReference type="GeneID" id="123401881"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0470090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0470090.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0470090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0567190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0567190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0567190"/>
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    <dbReference type="KEGG" id="hvg:123401881"/>
    <dbReference type="OrthoDB" id="838391at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
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        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF198">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN SAUR72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ85940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0567190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="12501" checksum="D43DDF0126004A5B" modified="2011-05-31" version="1">MKRLFRRLSRVAAADSSSAAAATAYRQLRSAPKQPSAAGGGKVPQGHVPVCVGEEGGPVERFAVRADLLGRPAFAALLLRAAQEYGYGHPGALRIPCPVADFRRLLVRLSDDPYAADC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTG8</accession>
    <name>F2CTG8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123403124</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EMBL" id="AK354920">
        <property type="protein sequence ID" value="BAJ86139.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953022.1">
        <property type="nucleotide sequence ID" value="XM_045097087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CTG8"/>
    <dbReference type="SMR" id="F2CTG8"/>
    <dbReference type="STRING" id="112509.F2CTG8"/>
    <dbReference type="PaxDb" id="4513-MLOC_6677.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0552540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0552540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0552540"/>
    </dbReference>
    <dbReference type="GeneID" id="123403124"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0459050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0459050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0459050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0552540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0552540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0552540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403124"/>
    <dbReference type="eggNOG" id="KOG3210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_0_0_1"/>
    <dbReference type="OMA" id="RWHQYFV"/>
    <dbReference type="OrthoDB" id="2039at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CTG8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000038">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF1">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="144"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ86139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0552540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="256" mass="27195" checksum="A77BFEE07BE8E5AD" modified="2011-05-31" version="1">MAAVVGVLALQGSYNEHMAALRRIGAKGVEVRKPEQLLAVDSLIIPGGESTTMAKLANYDNLFPALREFVGTGKPVWGTCAGLIFLANKAVGQKTGGQELVGGLDCTVHRNFFGSQLQSFETELSVPMLAEKEGGSNTCRGVFIRAPAILEVGQDVEVLADCPVPAGRPSITITSGEGVEDQVYSKDRVIVAVRQGNILATAFHPELTSDSRWHQLFLDMDKESQAKALAALSLSASSNNAEVGSKNKAPDLPIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2CW55</accession>
    <name>F2CW55_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ubiquitin-related modifier</fullName>
            <shortName evidence="8">SUMO</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123445214</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK355858">
        <property type="protein sequence ID" value="BAJ87076.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK369215">
        <property type="protein sequence ID" value="BAK00417.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371587">
        <property type="protein sequence ID" value="BAK02785.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978107.1">
        <property type="nucleotide sequence ID" value="XM_045122172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CW55"/>
    <dbReference type="SMR" id="F2CW55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309820"/>
    </dbReference>
    <dbReference type="GeneID" id="123445214"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0258670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0258670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0258670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0309820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445214"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ87076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAK00417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="101" mass="11234" checksum="4FB012AD695FD3AC" modified="2011-05-31" version="1">MSGVTADEDKKPAGDGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDLNSIAFLFDGRRLRGEQTPDELEMEEGDEIDAMLHQTGGCFRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2D1K6</accession>
    <name>F2D1K6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123427005</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357763">
        <property type="protein sequence ID" value="BAJ88977.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966869.1">
        <property type="nucleotide sequence ID" value="XM_045110934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D1K6"/>
    <dbReference type="SMR" id="F2D1K6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0175560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0175560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0175560"/>
    </dbReference>
    <dbReference type="GeneID" id="123427005"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0145090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0145090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0145090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0175560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0175560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0175560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427005"/>
    <dbReference type="OrthoDB" id="643388at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000009">
        <property type="entry name" value="NAC domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044799">
        <property type="entry name" value="SOG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31079:SF52">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31079">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="134"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ88977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0175560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="442" mass="49456" checksum="1254CFF4378ACECB" modified="2011-05-31" version="1">MASRVHAEPAPDPREARRQSRVGFACLLPWAKHFGHSLRLFKGRAHGRLGHPPGSFPPHPPRMLVRSLLPHHSATPEPPQRGRAPAMDEPFIVTGKIIAMMIRNGELVAEAGMECPKCEHRIDNSDVSSQWPGLPIGVKFDPTDLELLGHLEGKISRAVPHVLIDDFIPTIGKEEGICYTHPENLPGVKLDGIASHFFHKIGNAYDVGTRKRRKISNNYHTVCDERLRWHKTGKSRSILDNNGVIKGWKKIMVLYMGSKKGGGKTKKTNWRMHQYHLGVDEDEKDGELVVSKVFYQSESEKAGQPLIWCGNEEYDSFAGENDPPTPMTYPPEPCRPNDSPFETEQNQEEDEEFRQSAVRGAKEWLAGTTSHAVNDMALPGLDEHPSRGGTPDYPENSEKQHLPLDAEELQGFPDLGTSPHFSSLAGMKFDSLDGFHSWLKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDD8</accession>
    <name>F2DDD8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446860</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK361905">
        <property type="protein sequence ID" value="BAJ93109.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979336.1">
        <property type="nucleotide sequence ID" value="XM_045123401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDD8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398160"/>
    </dbReference>
    <dbReference type="GeneID" id="123446860"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0331280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0331280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0331280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0398160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446860"/>
    <dbReference type="HOGENOM" id="CLU_129534_4_1_1"/>
    <dbReference type="OrthoDB" id="661559at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181:SF55">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181">
        <property type="entry name" value="OS01G0778500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ93109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="86" mass="10332" checksum="C67997473904ED09" modified="2011-05-31" version="1">MGCWWERLVLPVRRAWLGVASRFGVRQSGLWRLRQEVSTCEYEDVRVMWEMLSRTASAPPPPPARRHSRFRQPRPWADRLRLCRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFR5</accession>
    <name>F2DFR5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123409747</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK362732">
        <property type="protein sequence ID" value="BAJ93936.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK374231">
        <property type="protein sequence ID" value="BAK05428.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958547.1">
        <property type="nucleotide sequence ID" value="XM_045102612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DFR5"/>
    <dbReference type="SMR" id="F2DFR5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713870"/>
    </dbReference>
    <dbReference type="GeneID" id="123409747"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0592120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0592120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0592120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0713870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409747"/>
    <dbReference type="OrthoDB" id="514822at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467">
        <property type="entry name" value="ZINC-FINGERS AND HOMEOBOXES RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="253"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="255"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ93936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK05428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="329" mass="36648" checksum="54BA5BE2CF57CAAE" modified="2011-05-31" version="1">MATTVVPLAAAMAAAPRARFLGAPDPFPLLATRPRLCLSPRGVACALRRPSKYKNRIKNEVVVAEDDIDGGDEDDDDEGGLEALFKQLEEDLKNDDLSVEDDDDNEISEEDMARFEQVLAEAMGDIDVDESAVDLVPSSEVVDNDEVADPVVKPELKSWQLRRLARALNIGRRKTSIKNLAGELGLDRGLVIELLRNPPPELLLMSDSLPDEPPSKPETKEIEPSPVADEVEVDEIEATETNAQMDLPIHVMSTEWSAQKRLKKAQLETLEKVYFKSKRPTNTMISSIVQVTSLPRKTIIKWFEDRREQDGVPDRHAAYKRPLSETMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F2DKE9</accession>
    <name>F2DKE9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123401664</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK364367">
        <property type="protein sequence ID" value="BAJ95570.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK369884">
        <property type="protein sequence ID" value="BAK01085.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951443.1">
        <property type="nucleotide sequence ID" value="XM_045095508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DKE9"/>
    <dbReference type="STRING" id="112509.F2DKE9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580120"/>
    </dbReference>
    <dbReference type="GeneID" id="123401664"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0481130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0481130.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0481130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0580120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401664"/>
    <dbReference type="eggNOG" id="ENOG502R5UY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ANGLRCH"/>
    <dbReference type="OrthoDB" id="675006at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DKE9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021899">
        <property type="entry name" value="DUF3511"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33193">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (DUF3511)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33193:SF71">
        <property type="entry name" value="OS02G0223700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12023">
        <property type="entry name" value="DUF3511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ95570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="188" mass="19922" checksum="B1E8B7A791B6C9D7" modified="2011-05-31" version="1">MPMERSVSLAERSTVAAPANDLRCHSASYAASYVPTKVQRARSTTSVAAPAVQRSGSTRTVSGAGPTPGLNLRCYSASYAASYKPFSDGAAQAKGPNTTTAAPTWCSAGRRSLNLRSYTPSFSALVDNDAPAPAKTAAAAPDDADAELQRKKRLVAYKVYDVEGKVKGSVRRSVKWIKVKCSRAVYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2DN26</accession>
    <name>F2DN26_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429067</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAJ96497.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366037">
        <property type="protein sequence ID" value="BAJ97240.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045113127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DN26"/>
    <dbReference type="SMR" id="F2DN26"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097950"/>
    </dbReference>
    <dbReference type="GeneID" id="123429067"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0080800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0080800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0080800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0097950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097950.1"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF82">
        <property type="entry name" value="APYRASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDA1_CD39_NTPASE"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="448"/>
        </location>
    </feature>
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        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
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        </ligand>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ96497.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="448" mass="48253" checksum="610CC5688F4F3B45" modified="2011-05-31" version="1">MANTLGIHSLVLVLLLLASSSAFADDVLDRKAGVAVEARPAPGKYAVIMDAGSTGTRVHVFRFDKKMELVDVGDDIEVFATVNPGLSSYAGRPQEAANSIVPLLEKANSAVPRSLMKRTPVKLGATAGLRLIGDEQAEQILEAVRGVVHTKSKFQYNPSWINVLEGSQEGSYLWVALNYLLDKLGGEYTKTVGVIDLGGGSVQMAYAISADAAATAPVVKDPYVTKEYLKGRDYNVYAHSYLHYGAMAARGEIFKAKNGPISYCMLRGFIGKYTYNGDKYDAIASPTGAAYDKCREDVIKALKLSAPCEAKNCTFNGAWNGGGGAGQADLYAASSFYYMASRVGLIDSDSTSGKTTPAAYRAAAEKICRLSLEEAKALYPRARATDVPYLCMDLVYQYSLLVYGFGLEPTKVITVVEKVKHGEYFIEAAWPLGEAIEAVSPTKRLQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQR5</accession>
    <name>F2DQR5_HORVV</name>
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        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LOC123453020</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="BAK06344.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045129776.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985712.1">
        <property type="nucleotide sequence ID" value="XM_045129777.1"/>
    </dbReference>
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    <dbReference type="STRING" id="112509.F2DQR5"/>
    <dbReference type="PaxDb" id="4513-MLOC_56030.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525860"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525860.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525860.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525860"/>
    </dbReference>
    <dbReference type="GeneID" id="123453020"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525860.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525860.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525860"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="VAPCDVK"/>
    <dbReference type="OrthoDB" id="1904536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DQR5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Outer arm dynein light chain 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000505">
        <property type="entry name" value="Outer arm dynein light chain 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF43">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="236"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="558"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ97436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="610" mass="64975" checksum="304CA5A7F815D9BC" modified="2011-05-31" version="1">MAKMSCFSILLTGRRQKRVQVGDAGKGAGTGNESPRAKPVAVESTDALPQPVAAAVVDKCGDKIVADVVIVVAHKAGEASPVKRDSLSDDFEFDFHPHPNKPVVSDVHGVDKQPLGTVPDAPVVTSGGEEAAAVEVDPSAKLKRSCSNIETKRTGPRDAPGMPVRSRSSGDLPGDLFVVTTPRRAHEASPDASVKTSRTADGVMLKRRSSSQVLPSRSRKLWWRLFLWSHRNLHRPWSARPSDAGTPGGGYTSDTLEEPADRKNKKVVVDESPPQPPSQNQWVAFCADHSLSDRVSEWVSSIDNSGCLRIAEEEDDDNGADQSMDLADDCVARPRAIEAGETSGKGGHGKLAKRCAAADDVAQANSIVQSLNGFSSVAHISGMGLKVVPMIAPFSNLRAVNLSGNFIVHISPGSLPKGLHSLDLSRNKIANVEGLRELTKLRVLNLSYNRISRIGHGLSNCTAIRELYLAGNKISDVEGLHRLLKLAVLDLGFNRLTTAKALGQLVANYHSLLALNLVGNPVQANVGDDALRKAVTDLLPQLAYLNKQPLKPREVATDSVARAALGGSGTGGRRRGASRRLSQSPGSSSSSSRSRSKGRQHHGSSVTARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2DRK3</accession>
    <name>F2DRK3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="7">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123431274</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the CCR4-NOT complex, at least composed of CRR4 and CAF1 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="7"/>
    <dbReference type="EMBL" id="AK366521">
        <property type="protein sequence ID" value="BAJ97724.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971029.1">
        <property type="nucleotide sequence ID" value="XM_045115094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DRK3"/>
    <dbReference type="SMR" id="F2DRK3"/>
    <dbReference type="STRING" id="112509.F2DRK3"/>
    <dbReference type="PaxDb" id="4513-MLOC_16273.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213970"/>
    </dbReference>
    <dbReference type="GeneID" id="123431274"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0177900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0177900.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0177900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431274"/>
    <dbReference type="eggNOG" id="KOG0304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027974_1_0_1"/>
    <dbReference type="OMA" id="WAYNLES"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000067">
        <property type="entry name" value="Putative CCR4-associated factor 1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAJ97724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="325" mass="34693" checksum="C93866F205F2FE2B" modified="2011-05-31" version="1">MKVQTEAAAGAGGEGKLVIRQVWAHNVESEFHAIRQAAERFPYVSMDTEFPGVIHCPSKHHASLTPSERYEALKANVDALHLIQVGLAFAASPDAPPSVAFEINLREFDPRVHRHNPRSVALLADHGLDFALQRRHGVDARVFSALLMSSGLVCSGAAAWVTFHSAYDFGYLVKLLMGRKLPRTLPEFIGLVRVFFGQQVYDARHVMDSCAGLYGGLDALAAQLGVERAAGMSHQAGSDAALTWDVFRRIREVYFANRQGLGAFAGVLYGLELDLALAPADNGGGKKCGAANANGNAVTANGTGTGTGKGRGGSNNNRRAVAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F2DRM4</accession>
    <name>F2DRM4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PHD finger protein ING</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123449682</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="AK366542">
        <property type="protein sequence ID" value="BAJ97745.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982916.1">
        <property type="nucleotide sequence ID" value="XM_045126981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DRM4"/>
    <dbReference type="SMR" id="F2DRM4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407110"/>
    </dbReference>
    <dbReference type="GeneID" id="123449682"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0338930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0338930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0338930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407110"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031900_4_0_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17015">
        <property type="entry name" value="ING_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15587">
        <property type="entry name" value="PHD_Yng1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000016">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="197"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="98"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ97745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="27941" checksum="49D08C2526E4CB4E" modified="2011-05-31" version="1">MGFLEDFQASVETLPAMLHKNYSLMRELDKSLQGVQLENEQRCQQEIEDIKHGLESGSITTYEPAKLKFSDEAIEEQKHCVRIADEKVALATQTYDLVDAHIQQLDQFMRKLEELRLEKEAAAAAAAAAAADAAVAATSAAPAGAGTFRSAAADPAPKTGRTGERSRGGRKKAKVPMELPTEQPPIDLELPVDPNEPTYCFCNQVSYGEMVACDNPDCKIEWFHFGCVGLKEQPRGKWYCLSCSAFQKKRKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXW4</accession>
    <name>F2DXW4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123407909</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK368732">
        <property type="protein sequence ID" value="BAJ99935.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371895">
        <property type="protein sequence ID" value="BAK03093.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957087.1">
        <property type="nucleotide sequence ID" value="XM_045101152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXW4"/>
    <dbReference type="SMR" id="F2DXW4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0702010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0702010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0702010"/>
    </dbReference>
    <dbReference type="GeneID" id="123407909"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0582310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0582310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0582310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0702010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0702010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0702010"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407909"/>
    <dbReference type="HOGENOM" id="CLU_078927_0_0_1"/>
    <dbReference type="OrthoDB" id="515493at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11446">
        <property type="entry name" value="bHLH_AtILR3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057075">
        <property type="entry name" value="bHLH_IRO3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044818">
        <property type="entry name" value="ILR3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133:SF6">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23177">
        <property type="entry name" value="bHLH_IRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="70"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="111"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ99935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0702010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="240" mass="26300" checksum="56C6F75960260AB8" modified="2011-05-31" version="1">MSLPPTDGGDDWFLDCGILEDLPAAACGAFPWDASVSSSNPSAEVGSYVNNSDVPKDSSSNKRLRSGPCGRPTSKACREKVRRDKLNDRFLELGTTLDPGKPVKADKAAILSDATRMVTQLRAEAKQLKDTNGSLEDKIKELKAEKDELRDEKQKLKLEKETLEHQMKLLTATPAYMPHPTMMHSPFAQAPMAPFHAQGHASAQKLMMPFVGYPGYPMWQLMPPSEVDTSKDSEACPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0P3</accession>
    <name>F2E0P3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123446940</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369714">
        <property type="protein sequence ID" value="BAK00915.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979421.1">
        <property type="nucleotide sequence ID" value="XM_045123486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0P3"/>
    <dbReference type="SMR" id="F2E0P3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333250"/>
    </dbReference>
    <dbReference type="GeneID" id="123446940"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446940"/>
    <dbReference type="HOGENOM" id="CLU_046006_12_3_1"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47802">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47802:SF1">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK00915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="137" mass="15045" checksum="1EFC4DCAB862712D" modified="2011-05-31" version="1">MATLQLNHIARETGDVRRLAAFYEEVLGFERVPSHAYSGFQVAWLRLPASPDVALHIIERDPAVASPGAEGVPPSQLPRRHHLAFSVADFDGFLTGLRTRGTELFEKTQPDGLTRQVFFFDPDGNGLEVTSSSPGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F2E2Z2</accession>
    <name>F2E2Z2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123443278</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK370515">
        <property type="protein sequence ID" value="BAK01714.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975545.1">
        <property type="nucleotide sequence ID" value="XM_045119610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E2Z2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0249580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0249580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0249580"/>
    </dbReference>
    <dbReference type="GeneID" id="123443278"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0207260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0207260.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0207260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0249580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0249580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0249580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443278"/>
    <dbReference type="OrthoDB" id="685087at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF3">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK01714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0249580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="412" mass="43596" checksum="2D9D0B1FBC2B817D" modified="2011-05-31" version="1">MPQQQPPLPLPPRMRPPTDREPAMAGPPPPRQRLGQAPRPFRAAEEPTARGPPPVVADRARPPEPRPRPSLAEEGRASHEALLANVARPAGRQEGRAAREAPAAAAVARPPEQQHGAPEGRATREAPVVPVARPPEKLHDAPEGRKAPRDAPIAPVARSPEQLYGSPEVRAAREAPVATVARPPEPRPGPYYVPGGGAAAPVATTDRPPETSPSPWPYPYYYEPEQRPPRRRTSALASCLMAAAFLLFAAGGAATALFLLFRPQPPEIAVAAVRLPSFAAANGTVAFTFEQVASVRNPNRAPLAHFDSSLRVAYAGGEIGSIYIPAGLIDSGSTKHWSTTFAVPAFPAATPPPLDMAAQQPAAAAAVMEVDSLLVVKGKVMVLRVLTHRVQASKVCRVGVSPIDGRVLGFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F2E4R2</accession>
    <name>F2E4R2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123424815</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371136">
        <property type="protein sequence ID" value="BAK02334.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964446.1">
        <property type="nucleotide sequence ID" value="XM_045108511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E4R2"/>
    <dbReference type="SMR" id="F2E4R2"/>
    <dbReference type="PaxDb" id="4513-MLOC_4602.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0115250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0115250"/>
    </dbReference>
    <dbReference type="GeneID" id="123424815"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0094680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0094680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0094680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0115250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0115250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0115250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424815"/>
    <dbReference type="eggNOG" id="KOG1965">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_11_2_1"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E4R2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF117">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="15">
        <location>
            <begin position="32"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAK02334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="538" mass="59246" checksum="AEB0B9A1B1016AE3" modified="2011-05-31" version="1">MAFEVVAAQLARLSDALATSDHASVVSINLFVALLCACIVLGHLLEENRWLNESITALIIGLCTGVVILMTTKGKSSHVLVFSEDLFFIYLLPPIIFNAGFQVKKKQFFRNFMTITLFGAVGTMISFFTISLAAIAIFSKMNIGTLDVSDFLAIGAIFSATDSVCTLQVLNQDETPFLYSLVFGEGVVNDATSVVLFNALQNFDPNQIDAIVILKFLGNFCYLFVSSTFLGVFSGLLSAYIIKKLYIGRHSTDREVALMMLMAYLSYMLAELLDLSGILTVFFCGIVMSHYTWHNVTESSRVTTKHAFATLSFIAETFLFLYVGMDALDIEKWKFASDSPGKSIGISSILLGLVLVGRAAFVFPLSFLSNLTKKTELEKISWRQQIVIWWAGLMRGAVSIALAYNKFTRSGHTQLHGNAIMITSTITVVLFSTMLFGILTKPLIRFLLPASSNGDPSEPSSPKSLHSPLLTSMLGSDMEAPLPIVRPSSLRMLITKPTHTIHYYWRKFDDALMRPMFGGRGFVPYSPGSPTDPNVIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECY7</accession>
    <name>F2ECY7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK374012">
        <property type="protein sequence ID" value="BAK05209.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECY7"/>
    <dbReference type="SMR" id="F2ECY7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0723450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0723450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0723450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0599860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0599860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0599860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0723450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0723450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0723450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014476">
        <property type="entry name" value="AHL15-29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100:SF20">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016021">
        <property type="entry name" value="ESCAROLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="3">
        <location>
            <begin position="102"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR016021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK05209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0723450.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="285" mass="29304" checksum="346DF40271319970" modified="2011-05-31" version="1">MATGSSKWWQGPMDFPPQPQQMQQHQHQQLQLPAVTMPAPAPAVAASPENKQQQQQQQGQGQGEGQMGAAAGAIVPLRRPRGRPMGSKNKPKPPIIITRDSPDALHSHILEVASGADVAACVAEYARRRGRGVCVLGASGSVVDVVVRGAAAPAPLPGRFELLSMTGTVLPPPAPSEASGLAVMLSAGQGQVLGGCVVGPLVAAGTVTLFAATFANAVYERLPLQDAAPDADVKPDLSAAPDASVSQQVQAQQPLAISQAMAMGAGYPDHRSPQYPWGGHQGGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2EF66</accession>
    <name>F2EF66_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123448275</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK374792">
        <property type="protein sequence ID" value="BAK05988.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981060.1">
        <property type="nucleotide sequence ID" value="XM_045125125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EF66"/>
    <dbReference type="SMR" id="F2EF66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0358390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0358390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0358390"/>
    </dbReference>
    <dbReference type="GeneID" id="123448275"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0299000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0299000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0299000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0358390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0358390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0358390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448275"/>
    <dbReference type="HOGENOM" id="CLU_124696_0_0_1"/>
    <dbReference type="OrthoDB" id="1933874at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052674">
        <property type="entry name" value="SelWTH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33638">
        <property type="entry name" value="SELENOPROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33638:SF1">
        <property type="entry name" value="SELENOPROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK05988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0358390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="20522" checksum="BA592758533E0F01" modified="2011-05-31" version="1">MPPKRKSPVATAALPAMPPRMTRSMAAGKRGADAPAKKEEAEAAAAAAAGQKGRKKAKKEVSAVAKEEEETVLSPPAAAKQKVKKNAKKEVVAAAVDDGGAGVENGKRVIVEACTQCQQFKRRALKVKEDLESAVPGVSVTINPEKPRRGCLEIREEGGDVFISLLNMPRPFNKMRELDMDKVIKDIAQKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2EIL4</accession>
    <name>F2EIL4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123452135</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Seed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK375991">
        <property type="protein sequence ID" value="BAK07186.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984662.1">
        <property type="nucleotide sequence ID" value="XM_045128727.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984663.1">
        <property type="nucleotide sequence ID" value="XM_045128728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EIL4"/>
    <dbReference type="SMR" id="F2EIL4"/>
    <dbReference type="STRING" id="112509.F2EIL4"/>
    <dbReference type="PaxDb" id="4513-MLOC_71738.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0508840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0508840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0508840"/>
    </dbReference>
    <dbReference type="GeneID" id="123452135"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0422550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0422550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0422550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0508840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0508840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0508840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452135"/>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_0_1"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EIL4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000232">
        <property type="entry name" value="Organic cation/carnitine transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="22"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK07186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0508840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="481" mass="52725" checksum="14EBA561CD235D7D" modified="2011-05-31" version="1">METYTTDDALSAMGFGKFQGLVLAYAGMGWVVEAMELNLLSFVGPLVREEWKVSAQDESLLSSVVFAGMLIGSLAWGFVSDRYGRRTVLLFSALFTSGMGFLSAWSPNYLCLMALRFLVGVGVGGGHVFSSWFLEFVPAQNRGTCVVLFSLFWTIGTILEASLAWVVISALNWRWLLVLTSLPCFLLLPFFRIIPESPRYLCVQNRMSDAILVLESVSMINQAALPPGVLTYRRENKVDTLTSETEVDHLLPVRETEIECKANNALSFRSGAVAALRRLLSPALCRSTLLLWFVYFANSFAYYGVVLLTSQLSDANRNCPSGLTNIQHQEDANLYRDTFVTSLAEIPGLIVSAVLVEWIGRKATMWSMLFTCCAFLGPLVLHQNELFTTALLFGARACAMGSSTVLCLYAPEVYPTSVRSTGVGIATSIGKIGGVICPLVAVGMLRTCHQMEAVFVFELVLCLAGVACVLFPVETKGREID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKJ5</accession>
    <name>F2EKJ5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444970</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Seed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376672">
        <property type="protein sequence ID" value="BAK07867.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977804.1">
        <property type="nucleotide sequence ID" value="XM_045121869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKJ5"/>
    <dbReference type="SMR" id="F2EKJ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303530"/>
    </dbReference>
    <dbReference type="GeneID" id="123444970"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0253310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0253310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0303530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444970"/>
    <dbReference type="HOGENOM" id="CLU_066247_13_1_1"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000035">
        <property type="entry name" value="Nuclear transcription factor Y subunit B-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF171">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="6">
        <location>
            <begin position="23"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK07867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="165" mass="17843" checksum="BF14B43A3F233681" modified="2011-05-31" version="1">MADDDSGSPRGGGGGGVREQDRFLPIANISRIMKKAVPANGKIAKDAKETLQECVSEFISFVTSEASDKCQKEKRKTINGDDLLWAMATLGFEEYVDPLKIYLQKYRDMEGDSKLTSKSGDGSVKKDIIGAHGGATSSNAQVMVQHGAYAQGMGYMQPQYHNGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL48</accession>
    <name>F2EL48_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ninja-family protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ABI-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449317</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a negative regulator of abscisic acid (ABA) response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Ninja family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376876">
        <property type="protein sequence ID" value="BAK08070.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982434.1">
        <property type="nucleotide sequence ID" value="XM_045126499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EL48"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396620"/>
    </dbReference>
    <dbReference type="GeneID" id="123449317"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396620"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031307">
        <property type="entry name" value="Ninja_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012463">
        <property type="entry name" value="Ninja_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413">
        <property type="entry name" value="AFP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413:SF49">
        <property type="entry name" value="NINJA-FAMILY PROTEIN MODD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07897">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ethylene-responsive binding factor-associated repression" evidence="6">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Tify" evidence="7">
        <location>
            <begin position="345"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK08070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="39204" checksum="7C8CE1413E5ADF37" modified="2011-05-31" version="1">MEAYSKDMLRGVGAGDDGEEKVQRSASRTEEVDLSLGLSLGGRFGAERRSGDGLAGSSSAVSIQEVAPAPPAPVIRTNTAPTMVYAGAGSSTATAFLRNGAVVQRSATTPTMAYAGAGSSTATAFLRNGAVVQPSATVPQGSGVPVNGVVVEPLALLRATVSAFLGSAGQVRPSATPGSCFPLTLSSIKEEPVANMPSQGSPAGQGILTNGGIRNHGSLPAGIEGWLRMRKSKNLKRLETKRRIQGLKPSMIPDASQGTPGAEGRSLSAAVMSWPVGTNGVVATASSSASAVVAAALGSGGERQHAPDMPAMMVQEMPLVWTIGLPNGRRAVGWLYEYSKVDEVRIKCVCHGSFFNPAQFVEHAGGGQVANPLRSILVKPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2ELV2</accession>
    <name>F2ELV2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123448838</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Probable glycosyltransferase that may be involved in the biosynthesis of xyloglucan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK377130">
        <property type="protein sequence ID" value="BAK08324.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981795.1">
        <property type="nucleotide sequence ID" value="XM_045125860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ELV2"/>
    <dbReference type="SMR" id="F2ELV2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0382670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0382670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0382670"/>
    </dbReference>
    <dbReference type="GeneID" id="123448838"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0318300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0318300.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0318300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0382670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0382670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0382670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448838"/>
    <dbReference type="HOGENOM" id="CLU_034328_1_1_1"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035252">
        <property type="term" value="F:UDP-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033843">
        <property type="term" value="F:xyloglucan 6-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009969">
        <property type="term" value="P:xyloglucan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000101">
        <property type="entry name" value="Probable glycosyltransferase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31311:SF22">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31311">
        <property type="entry name" value="XYLOGLUCAN 6-XYLOSYLTRANSFERASE 5-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK08324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0382670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="456" mass="51635" checksum="D9A6E5CF39E0090A" modified="2011-05-31" version="1">MPLPSSAMAEEHAGDQHEQQVAAERRARPKLLLAVPIIPIVLFIFAPCALFLFTTDLALPRIRIEYARRDGDRDAPASALAKTLPPPPPKAMVVVPSEEQQQLPPLRQLTDRPYSLGPNVSDYDARRAAWLAAHPRFPAFVAPGRPRVLVVTGSSPRRCKDPEGDHVLLRAFKNKADYCRVHGFDIFYSNAVLDSEMSGFWTKLPLLRALMVAHPEVELLWWVDSDVVFTDMLFEPPWGKYARHNLLLHGWDDAVYGARNWLGTNAGSFVIRNCRWSLDLLDAWARMGPRGPVRDRYGEVFAEALSNRAAYEADDQSALVYLLVTERGRWGPKVFLESSYLLHGFWEGIVDRYEEMRSKWRPGLGDDRWPLVTHFVGCKPCGEQSASYEAARCRQGMERALNFADDQILGLYGFQHQSLNTTAVRRVRNDTGRPLDADDEQIGRLLHPEFRAAKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>J7GIU6</accession>
    <name>J7GIU6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar invertase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123413222</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Plant Mol. Biol." volume="80" first="299" last="314">
            <title>Clusters of genes encoding fructan biosynthesizing enzymes in wheat and barley.</title>
            <authorList>
                <person name="Huynh B.L."/>
                <person name="Mather D.E."/>
                <person name="Schreiber A.W."/>
                <person name="Toubia J."/>
                <person name="Baumann U."/>
                <person name="Shoaei Z."/>
                <person name="Stein N."/>
                <person name="Ariyadasa R."/>
                <person name="Stangoulis J.C."/>
                <person name="Edwards J."/>
                <person name="Shirley N."/>
                <person name="Langridge P."/>
                <person name="Fleury D."/>
            </authorList>
            <dbReference type="PubMed" id="22864927"/>
            <dbReference type="DOI" id="10.1007/s11103-012-9949-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="JQ411255">
        <property type="protein sequence ID" value="AFP72241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962100.1">
        <property type="nucleotide sequence ID" value="XM_045106165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="J7GIU6"/>
    <dbReference type="SMR" id="J7GIU6"/>
    <dbReference type="STRING" id="112509.J7GIU6"/>
    <dbReference type="PaxDb" id="4513-MLOC_37242.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635200"/>
    </dbReference>
    <dbReference type="GeneID" id="123413222"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0527740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0527740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0527740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0635200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413222"/>
    <dbReference type="eggNOG" id="KOG0228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="202537at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="J7GIU6">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050551">
        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018053">
        <property type="entry name" value="Glyco_hydro_32_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00609">
        <property type="entry name" value="GLYCOSYL_HYDROL_F32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="8">
        <location>
            <begin position="123"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="9">
        <location>
            <begin position="464"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFP72241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="639" mass="70307" checksum="0093A260F7A7616B" modified="2012-10-31" version="1">MAPRGRRTSRSKDFLLSSGTAAVAVKPRGAVLDTPPVPLAYGVLKYQQSGGGTRWRECTVVLGAVAMVALFVTHALAPRASVLSEETRGAARPEQNIMVAAGSDADGFPWSNEMLQWQRTGFHFQPEKNYMNDPNAPMYYRGRHHFFYQYNPTGVVWGNITWGHAVSRDLVHWRHLPLAMVPDQWYDIHGVLTGSATILPNGTVIVLYTGKTDTSAQVQCLAMPTDPDDPLLVNWTKHPANPVILPPPGIGLQDFRDPTTAWFDNSDLTWRTIIGSKDDNGHAGIALMYKTKDFIRYELIQGVLHRVEGTGMWECVDFYPVGGGNSSSEEEAMYVLKASMDDERHDYYALGRYDAATNTWTPLDPELDVGIGLRYDWGKFFAATSFYDPVKRRRVMWAYVGETDSLSADVAKGWASVQTIPRTVVLDEKTRTNLLQWPVEEIETLRFNSTDFGVITIHTGSIIPLCLRQATQLDIEASFRLDDSAIAMINEADVNYNCSTSSGASTMGALGPFGLLIHAAANGGSEQLAVYFHVSRGLDGALRTHFCHDELLSSRASDVMKRVVGSTVPVLDGEALCVRVLVDHSIVESFAMGGRLTATSRVYPMEAIHTAAGVYLFNNATGSSITVEKLVVHEMASTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0US85</accession>
    <name>M0US85_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123452094</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May be involved in cooperative interactions with calmodulins or calmodulin-like proteins. Recruits calmodulin proteins to microtubules, thus being a potential scaffold in cellular signaling and trafficking. May associate with nucleic acids and regulate gene expression at the transcriptional or post-transcriptional level.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984611.1">
        <property type="nucleotide sequence ID" value="XM_045128676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0US85"/>
    <dbReference type="FunCoup" id="M0US85">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0US85"/>
    <dbReference type="PaxDb" id="4513-MLOC_13673.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0507950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0507950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0507950"/>
    </dbReference>
    <dbReference type="GeneID" id="123452094"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0507950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0507950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0507950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452094"/>
    <dbReference type="eggNOG" id="ENOG502QT9B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0US85"/>
    <dbReference type="OrthoDB" id="654277at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0US85">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF31">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="276"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="377"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="402"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0507950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="438" mass="48198" checksum="4FC63E6773B9BA39" modified="2013-04-03" version="1">MGISAKWIKSLVRIRKQEKGRGSENQEKAENAESSEASSSARQLHKRKHSLDLAVEELAVPNETSTDGTNTQLGSNSVSSESASRDAHVSLTEELLTEGDLAATVIQSAFRAFLARRALRALKGIVLLQALVRGHIVRKQTAETLQCMHELVRAEARVRARQAGVALENQVARKKVPEQDDCENHVREIEEGWCGGIGSVAEMQAKVLKRQEAAAKRERAMAYALTHQRQAGLRQQKPTNLQGSELDDDHWGSNWVERWVAARPWENRLLDNNAKESMPMCDDNQDEEIKPQVTPKGKAPTSSTVTPPNGLNKKKGANHRKSYSDVNFTSFGRSSSVLPSTSLGTSKQKQKSDDEAFEEVSSQPIDIASLAVLNQKERRGQLNTSAKKRLSLPNNVGGGASKETTNRNPTKRSISAKSDPKPRANAPNQARKQVELQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0W2J9</accession>
    <name>M0W2J9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123424951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0W2J9"/>
    <dbReference type="SMR" id="M0W2J9"/>
    <dbReference type="FunCoup" id="M0W2J9">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W2J9"/>
    <dbReference type="PaxDb" id="4513-MLOC_4138.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0021020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0021020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0021020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0016520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0016520.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0016520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0021020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0021020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0021020"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W2J9"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W2J9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000710">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000996">
        <property type="entry name" value="Small kernel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF898">
        <property type="entry name" value="OS07G0436600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0021020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="59354" checksum="81B952B0B0B3CFCD" modified="2013-04-03" version="1">MVGHGVLLIFYFFAAQYICLPVAVSHTRGGSMRPLPAFAAARLAAVRADPRGVPLPVFNSLLSNLASSDPSHAHLPLHLFRRLLLLLPARRPDAFTLSSLASSFLPLHPSRPRSHAAASLHAFSLRLGLLHADPVLTNSILLLYLRSPNHPITPLRLFDEMPSRTASTYNTLISYAPAGTDLRSLARRMVADGLCPDRFTVSALLSACASERDGRELHCFAIKCGMCGGDGDFHVSSGFVSMYCRVSRPGLAHRVFYGMRQRNVVSWTAMVGGYAENGMFEDAAKAFRAMWAVDGILPNRVALISVLSAVEGLMGLAEGKQVHGFAVRMGLYGEVSLNNALIDMYAKAGALRYARRVFDDATWQKDVISWGSMVLGYGLHGMGTEAVTLFDHMHASGVKPDSIVGLGVLSACCRAGLVLKGLEIYNSLVKDHRVHPTEEMSACVVDLLGRSGLIDHALDFIKSMSVEPGPGVWGALLDASVIHSNKETQDLACRSLLRLEEESPSNLVSVSNLHASSGRWNIVEQVRAKIKHGTLKKTPGRSWVNAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>M0W9F3</accession>
    <name>M0W9F3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123409748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045102613.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031421">
        <property type="entry name" value="DUF4666"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33730:SF37">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33730">
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="15511" checksum="F764DEEA35C602D6" modified="2013-04-03" version="1">MEGLQRSSSTFRRSGSSGLVWDERFLTEGAEAKAAGEGGTDEAQPEMRRSRSTGGVGMMLRRGGDDKKQQEQQQKKEQGLKKKDEGRDQQVFRTKEVAPDMDPPSPRVSGCILCAIFGGSGSGSGPAARRGRAKPKRRQRSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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    </gene>
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        <lineage>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045110065.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="123426266"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="FACTOR OF DNA METHYLATION 1"/>
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    </dbReference>
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    </dbReference>
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        <location>
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    </feature>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="74186" checksum="2C3B2169998AAD5D" modified="2013-04-03" version="1">MEHTSEEESEISDSEIDEYKDKIYAQLRSQKLKVRYGEKIFRCPFCLGKKKQDYNVKDLLQHASGIGAALKRKPRVRAAHLALAEYVKNDLGSSLEPSLQLAIVEYKPPKIEEEKFVWPWMGILVNLTTDLMDTNSVRESEHMLKSQLSRFRPCEVTILLDSKGQTDHSIIKFAEDWTGLKDALAFEKHFIVEQYSKTDWNRRNCRMDDLYGWLARSDDYNSHGTIGEHLRKIGVLKSIGDREHERIERIAHFTHQMEEKNKHVLDLELKNSQNAMKLDSMMKDKDRMVEEHNEKIRKMQEGARRNSSKIVEDNQRLQQELKTKREQVIRRHKQLEELARKSNIDRAKVEAEKEKNANENALLDLATLKHKKAREELRQLLKKHEQEKEDAFRRQYKLEEDLTSKQNLEMELAQLRGKLEVMKHMGAEADTTSKEFDKVSEELKEKDEELDAMESENQALIIMERRTNDELEQAKKELIDGLQQIQVTRSTIGVKRMGVLDEKAFIAACKKKAGNVVSKKKAKYDVEATLVLSRWEDEIMQPGWHPYKVIDVDGQAKEIVKEDDEKLQALKEELGQEAHDVVVKALLELNENNPSGRYPVPALWNFKENRRAPLDEAVAYILKQWKASKGKRTYCR</sequence>
</entry>
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        </submittedName>
    </protein>
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                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
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    </reference>
    <reference evidence="6" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
    <dbReference type="STRING" id="112509.M0WU41"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF62">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="88" mass="9859" checksum="FDDC5AD9FC4FEC9F" modified="2013-04-03" version="1">MSSGSSSRSTSPSSDSGWSKKENKMFEEALAYYGEGAPNLWEKVASAMGGTKSAEEVRRHFQILIDDVNNIEYGRIPFPKYKTQGFWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0XD37</accession>
    <name>M0XD37_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0XD37"/>
    <dbReference type="SMR" id="M0XD37"/>
    <dbReference type="STRING" id="112509.M0XD37"/>
    <dbReference type="PaxDb" id="4513-MLOC_59002.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0231880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0231880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0231880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0192460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0192460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0192460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0231880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0231880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0231880"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XD37"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1181">
        <property type="entry name" value="OS08G0170200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="92"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="213"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0231880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="66927" checksum="D7D6221AF4571000" modified="2013-04-03" version="1">MRLYGDKSKCPFDHPLEMSEKMLQRCGGVPLAILTMASLLDDKAREDWSKVYDSIGFEHRKNQVVDNTRKILLFSYYDLPYYLRPCLLYLSIYPEDYTIYKDTLIWKWVGEGFIEEEQGIGQYELGERYFSELVNRSMIQPVWGSYGSCFLIGCRVHDLVLDMICLLSNEENFVRVWDVNDQRISYQINARRLAIQRRVLEKDDYLANMCTQQLRSFSVTQCDVHVMPSLSSFEALRVLDMEDCSSIADGIYHLGHLGSLIHLRFLGLRNMPIDKLPEEIGNLKFLQTLDLESTGIEELPQSFGLLRQLKCLHFKVREVTVGMHLLGNLTSLEKLLLVFDTWSPEFVAELGKLTMLRNLNLFSFDGRVLNDNQMKALVKSLVKLQKIEVLGIDFLYSVSIGHQDWEGYVPPRQLRELSLRTESNILPAWISPSLIPKVTSLSLMLKEVKARDMEILGSFPELITLWLSDQGFGNSQDFLPDVVGELFPKLRYFCTPAPLRFLQGAMPSLESLEYTYLQVNQLKRDRIFVFEFGSWENLHSLQKVKATIRPDAAHENEAVVALELVANQHPNRPNLTVNIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>M0XMD5</accession>
    <name>M0XMD5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box/kelch-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123401423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951156.1">
        <property type="nucleotide sequence ID" value="XM_045095221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XMD5"/>
    <dbReference type="SMR" id="M0XMD5"/>
    <dbReference type="FunCoup" id="M0XMD5">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XMD5"/>
    <dbReference type="PaxDb" id="4513-MLOC_6134.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614970"/>
    </dbReference>
    <dbReference type="GeneID" id="123401423"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0509890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0509890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0509890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0614970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401423"/>
    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XMD5"/>
    <dbReference type="OMA" id="YHIDRID"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000007">
        <property type="entry name" value="F-box/kelch-repeat protein SKIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052439">
        <property type="entry name" value="F-box/Kelch-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122:SF2">
        <property type="entry name" value="F-BOX_KELCH-REPEAT PROTEIN SKIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122">
        <property type="entry name" value="GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="46835" checksum="C811E9FD9755A9EB" modified="2013-04-03" version="1">MLEGQSCLISRSLPSSCEQESRLAYMTYHLLEITRTKRPSGVLAIEHDGIAVAAVLAKRPRSEDSHDAVPVDCQGSNQGYSDSSTLISSIGRDNSINCLARCSRSDYGSIASLSRNFRSLVRDGGLYRERRRLGIAEHWVYFSCNVQEWEAYDPYRGRWMTLPRMPPNECFMCSDKESLAVGTELLVFGKEILSHIVLSYSILTNSWSRGVEMNAPRCLFGSASFGEKAIIAGGMDSHGSVLSSAELYNSETKSWVTLASMNKRRRMCSGVFMDGKFYVIGGMASNTEVLTCGEEYDLEKGTWRLIENMSEGLNGASGAPPLVAVVENELYAAQYAGKLVRKYNKSDNTWTTLGELPERPEAVNGWGIAFRGCGERLLVIGGPRVLGGGMIELHSWIPREGPLQWNMIGSKPSGNFVYNCAVMGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y2L9</accession>
    <name>M0Y2L9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123444015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976544.1">
        <property type="nucleotide sequence ID" value="XM_045120609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y2L9"/>
    <dbReference type="SMR" id="M0Y2L9"/>
    <dbReference type="STRING" id="112509.M0Y2L9"/>
    <dbReference type="PaxDb" id="4513-MLOC_65158.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277120"/>
    </dbReference>
    <dbReference type="GeneID" id="123444015"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0229560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0229560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0229560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444015"/>
    <dbReference type="eggNOG" id="ENOG502QUHY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Y2L9"/>
    <dbReference type="OMA" id="ENFFQWD"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000030">
        <property type="entry name" value="F-box/kelch-repeat protein At3g61590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000076">
        <property type="entry name" value="F-box/kelch-repeat protein At3g61590 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056592">
        <property type="entry name" value="Beta-prop_At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24750">
        <property type="entry name" value="b-prop_At3g26010-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="402" mass="45734" checksum="43FBEF61D2C193EF" modified="2013-04-03" version="1">MEDDSSWEALPIERIRVPAFNFRVISEASNGENELPVSLDAVVPGDILEKIFTFLPIASMIRSTAVCKRWHDIIYSSRYLWTHMLPQRPWYFMFTCNETASGYAYDPILHKWYDLELQGIDKSSCFVSSSCGLVCFMDNDNRNIISVSNPITKDWKRLLEPPGAKFPDYSTVAIKVDQVTHNYTVTLAKSKQVPEDYVQWEFSLYRYDSWSSSWVTAVKEVFIGWRGGDDSVICGGVLYCLIQSTGVLGNVEPRHRLIMYDLVAGPSETSLTQSSIPVPCSLTCGRLLNLREKLVMVGGIAKPNRPDIIKGIGIWELDRTQWQEVSRMPHKFFQGFGELDDVFCSGGADHLVYIQSYGATALLGFDMKQRQWKWSAKCPVSKKFPLQLFTGFCFEPRLDITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y5A0</accession>
    <name>M0Y5A0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123412603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961489.1">
        <property type="nucleotide sequence ID" value="XM_045105554.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961490.1">
        <property type="nucleotide sequence ID" value="XM_045105555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y5A0"/>
    <dbReference type="PaxDb" id="4513-MLOC_6582.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637360"/>
    </dbReference>
    <dbReference type="GeneID" id="123412603"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0637360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637360"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412603"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="KADIDMG"/>
    <dbReference type="OrthoDB" id="695871at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y5A0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF193">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="1">
        <location>
            <begin position="837"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="998" mass="113960" checksum="D1DD807BEB22C2ED" modified="2013-04-03" version="1">MKREVIIMADTIDAAAEEILGILESMSKQLQSIYFDGWFRLGASSALRVVAQHLKSSAAQEMKSDKVLHVDCSLWQSMRALQKAVAEELELPQSVMAIFNQHDEEDDFNGIDQCSRGVIKDVREEIFRRLAGSSFIVIFHNGSSHYIDLYDCGIPVTTFLSNKVLWTWGGGFRLRGNLKGWDSIDFQRNTHMVPLAELDHALQEEAADVANRMGILDPDTIVECFQYVWKQRCAGQIDWGMHASNYWVCDGIIQGQGGTCAWEVGDGLFRNINLDWIEEYNYIGHVCISDYDDRWVLATHQDLLHDGIGVLPPEATSFFLLPEKSEGRVVLPAVMFPYNNRLHVLHLSWCTFSFTSPPFLCCRHLRFLLLDHCTDIKEEIQSNSQNRSCFQKLWVFDLRYTDWYSKTTMGLMDELRELNVERVKDWMSIVDICGSRSSLVKFRVAPDPDSPQEIIMHRQLTNLSSAIHLKTVILENCVGLEQVVPDVLPPLVESFSFILTDAAIPKISSISFRGLGKLKSVFLRGMMENLLELDLSGSAVKSLDLREVVALNLKQLIMMGCDKLKAIQWPSSDERTKKLEVLRIDTLRPASSGHANWEEKAKETSGATRSSCFVAVEVMDARLLRSLKPIRECFYRFGIGHMEMSSSPASSVVGGGSECGQGIRNPNQYLYARDIFIEDHLQAVAANEGLIEWIWDGPSDLAVDRFKHWYVHIQDEEEMKNGLLLQLQQQQDRTKYNSIGGALLPFWLCYGARTLHVHDSLYNTSIPSTRNSHWPQLEECRVERCPKLSFVFATPIKSEDGSNKSDTVGRFPQLTTFWASQLSMARYIWNWSTIQLSGEDSFQHLEFLHLDYCPRLIHVLPLSVHMTTLRHLATLEVVCCGDLMEIFPLDPTERQEKQTIINFPELKRIHLHDLPRLQHICGGKMFAPKLETIKTRGCWNLGRLPAVARSCPEVDCEKEWWDNLQWDEGDANHHPSLYKLCHSMYYKKAQLPRGTVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MA64</accession>
    <name>A0A287MA64_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123445156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978034.1">
        <property type="nucleotide sequence ID" value="XM_045122099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287MA64"/>
    <dbReference type="SMR" id="A0A287MA64"/>
    <dbReference type="STRING" id="112509.A0A287MA64"/>
    <dbReference type="PaxDb" id="4513-MLOC_19293.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308430"/>
    </dbReference>
    <dbReference type="GeneID" id="123445156"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0257510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0257510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0257510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0308430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308430"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287MA64"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287MA64">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="54100" checksum="0783908CDF381C56" modified="2017-11-22" version="1">MAASRRLARKLPSIISKHQRLISPETETPEENPEISTSPASIPLDPSLPLLPLAVSHLSPPSPLPSLPSAHASSPEALLRLLRRARHHPRLAPLDLHLLLAAADASSAFRPDHRLTSLLAARLAASRRLPSLRRLLQLVLSRPCPCADDSIFACPDLLPTFRKAILAFATSCDVPAASEALASLRRAADSPLPAEFYNIILHALARLHRHDDTIRFYGEMTSVHRVAPDAYTFNILINSSCRVQGVDAAMRWFEEMRRRSCAPTGVSFNTLMRGFFREGRHKEGLKVAHEMLQLGAGLSVASMEILIGGLCRGGEALKAAEVFQEFLVDAVVPEGYDCLDLVESLCHVRRVDKAIEVVELLLERNRASCLSVPAGVTVLECLMKAGRLDDVCRLMGRMAGEGIVPDTISCNCIFEALCEAGRTSDANRLRILAKENGFAADGATYNMLVQGFGRQGRVKEGEAVLDEMLDLGFIPNIVAYNRLLDSLHVRRSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287QTQ6</accession>
    <name>A0A287QTQ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rubisco LSMT substrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123398318</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948733.1">
        <property type="nucleotide sequence ID" value="XM_045092798.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948734.1">
        <property type="nucleotide sequence ID" value="XM_045092799.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948735.1">
        <property type="nucleotide sequence ID" value="XM_045092800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287QTQ6"/>
    <dbReference type="SMR" id="A0A287QTQ6"/>
    <dbReference type="STRING" id="112509.A0A287QTQ6"/>
    <dbReference type="PaxDb" id="4513-MLOC_13341.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0452010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0452010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0452010"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0452010.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0452010.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0452010"/>
    </dbReference>
    <dbReference type="GeneID" id="123398318"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0452010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0452010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0452010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0452010.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0452010.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0452010"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398318"/>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287QTQ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000003">
        <property type="entry name" value="Rubisco methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000003">
        <property type="entry name" value="Rubisco methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF9">
        <property type="entry name" value="RUBISCO METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015077418" description="Rubisco LSMT substrate-binding domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="5">
        <location>
            <begin position="377"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0452010.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="530" mass="58828" checksum="2C8026EC81B14FA4" modified="2017-11-22" version="1">MIFAWKIWLHLSRLSLIHTNRSSEARAPPMASSALSGTHHRLLLPCFLRRLPQPGSRSCSRLRLAACHADTLLSSSGAQGPPSPAACLSASSAGGFSDWLLTNGLPPGKLAILERPVPCSRGGRDRPLHFVAAGQDLEAGDVAFEVPMSLVVTLERVLGDESVAELLTTNKLSELACLALYLMYEKKQGRDSLWYPYIKELDRQRGRGQLAVESPLLWTESELDYLNGSPMRDEVVVRDEGIKKEYNELDTLWFMAGSLFKQYPFDVPTEAFPFEIFKQAFVAVQSCVVHLQKVSLARRFALVPLGPPLLTYKSNCKAMLTAVDGSVRLLVDRPYKAGEPIIVWCGPQPNSRLLLNYGFVDEDNPYDRIAIEASLNTEDPQYQEKRMVAQRNGKLAIQKFQVCVGKEKETISEMLPYLRLGYISDPDEMQCILSSEGDTCPVSPCSERAVLDQLVVYLKSRLAGYPTNLDEDEAMLADGSLEPKKEVATRLVRLEKKMLHGCLEAANEFISGLPDHTVSPCPALYAPELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W5C4</accession>
    <name>A0A8I6W5C4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclease associated modular domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123428665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968799.1">
        <property type="nucleotide sequence ID" value="XM_045112864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W5C4"/>
    <dbReference type="SMR" id="A0A8I6W5C4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0035140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0035140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0035140"/>
    </dbReference>
    <dbReference type="GeneID" id="123428665"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0035140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0035140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0035140"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003611">
        <property type="entry name" value="NUMOD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199:SF2">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07460">
        <property type="entry name" value="NUMOD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclease associated modular" evidence="2">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0035140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="555" mass="63031" checksum="7B944F2407433FDB" modified="2022-05-25" version="1">MAPQLLGYYWVSSWPLLPQFPPWPCASHAQRRRVICFGPPRCRAGAVRVIAEVDPSLALDRSAKTVEIQFPTEEDGVRNEEDGEAVDEREMMRRMRISQANKGNTPWNKGRKHSPETLQRIRERTRLAMQNPKVKKKLMNLGHAQSEETKIKISLGVRRGWSLRLQKLMVQDGCFVEWRKMIADAARKGFAGGIAFQWNSHKILTEQLRQEWLEKVQQRRSMPRPTGNRRAPKTPEQRRKIAEAIAAKWLDREYRERVCSAINSYHGTSSGSKVPRKQRTPREPGAKREKKKSIQHRAVSLDDAHARTAPVKRKKSATPYKDPMAGEKLEMITKIRAQRTALEIEKKEAIERARSLIVEAEKAANALEPVAAKNPFAQASLIEARKLVTEARISLKGVDDNDGPAESSSDDTSDDSGVSELHKYELENQNNLIKQENKSVNGMKLPPRTVNGMDFYFDVSALGEKEQLIMYQRIENSMERAYLLPSAFSTVQYVNGNLGTNGLYINKQVVNNDQIDQIAAETTEFISAEPLEDVSSPANKSKMRWVRGRLVEAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W9S7</accession>
    <name>A0A8I6W9S7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123429313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044969303.1">
        <property type="nucleotide sequence ID" value="XM_045113368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W9S7"/>
    <dbReference type="SMR" id="A0A8I6W9S7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106340"/>
    </dbReference>
    <dbReference type="GeneID" id="123429313"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0087160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0087160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0087160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0106340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429313"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000444">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001494">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000243">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF536">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="379"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="480"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="695"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="787" mass="86379" checksum="F5BA07AAF1912C34" modified="2022-05-25" version="1">MRPRLPATRLNPAAAPRRAPTARLLSTAAPPSDARHLQPQQQQQLEQLIARGDLSRARHLFDEIPDPDARAYNALIRAYSWRGPAHAAGALALYAAMLRDGRSPPNKYTFPFVLKACSALLDLRAGRAIHRHAARAGLHADLFVSTALIDLYMKCARFRPANAVFRAMPARDAVAWNAMVAGYALHGMYGHALECLLRMQEGRACLRPNASTLVALLPLLAQQSALRQGKSVHAYSVRASLHDKDGVLVGTALLDMYAKCGNLLYAQRVFEAMAVRNDVTWSAIIGGFVVCGRMLEAFILFKDMLAQGLGFLSPTSVASSLRACASLADVRIGKQLHVLLAKSGLQSDLTAGNSLLSMYAKAGLIDEATTLFNDMAAKDTVSYSALVSGYVQNGMADAAFLVFRKMQVCNVEPDVATMVSLIPACAHLAALQHGKCSHGSVIVRGMAPETSICNALIDMYAKCGRIAFSRQIFDAMPSRDIVSWNTMIAGYGIHGLGKEATALFLDMKNHACEPDGVTFICLISACSHSGLVTEGKHWFHMMAQKYGITPRMEHYISMVDLLARGGFLDEAYQFIQSMPMKADVRVWGALLAACRVHKNIDLGKQVARVIQKLGPEGTGNFVLLSNIFSAAGRFDEAAEVRIIQKEKGFKKSPGCSWIEINGSLHAFIGGDRSHPRSPEIYQELHNILVDINKLGYQADTSFVLQDLEEEEKEKALLYHSEKLAIAFGILTLSEDKDIFVTKNLRVCGDCHTVIKYMSLVRRRDIIVRDANRFHHFKNGQCSCGDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJ44</accession>
    <name>A0A8I6WJ44_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein IRON-RELATED TRANSCRIPTION FACTOR 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination. Promotes the accumulation of mugineic acid family phytosiderophores (MAs). Required for ethylene-mediated signaling during iron deficiency responses. Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJ44"/>
    <dbReference type="SMR" id="A0A8I6WJ44"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0170560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0170560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0170560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0204990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204990"/>
    </dbReference>
    <dbReference type="OMA" id="INCEELS"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000074">
        <property type="entry name" value="Transcription factor ORG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF41">
        <property type="entry name" value="TRANSCRIPTION FACTOR ORG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="74"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="27751" checksum="EA40B80D88EA6C2B" modified="2022-05-25" version="1">MDHLLYGDPSGSSFSPLEAQIFSGELPPSPTWPNLDLDLDLDLDVLEDDIVRELSERPAKAASSGSGSGGPGSHKKLSHNAYERDRRKQLNELYLSLRSLLPDADHTKKLSIPTTVCRALKYIPELQKQVENLEKKKEKLASANCKPGVLSVSGSIAPTVSATCLNDKEIMVQISMSRDKDAATTLPLSKCINVLENEGLQLISSSTSSTFGNKMFYNLHLQRSQGALNMECPSFCDKLEQAIRKTAGLYLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMX1</accession>
    <name>A0A8I6WMX1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMX1"/>
    <dbReference type="SMR" id="A0A8I6WMX1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0084500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0084500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0084500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0102730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000227">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000250">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035273850" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="22"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="26"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="363"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="521"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102730.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="818" mass="90682" checksum="EA56171D6145D0CA" modified="2022-05-25" version="1">MLPSFVVFVLVISVPPVACSAAANGSSTLTEGQELRGTDRLISKNGKFALGFFTSESSKSFSDNTTLPNWYLGIWFNKIPKFTHVWIANREKPISDPNFRLSKLRISRDGNLVILNHASKSIIWSSQIIIVNGRRRANKKKSVMAALSDNGNLVIRGASNTSIVWWQSFDHPTDVFLPGAKVGRNKVTGQKYSLVSKKNQINPAPGLYCVELDPTGGRQYFGKLCNSSIVYFSTGEWNGKYFNLVPESSSTNFFHIQFIDNDEEEYFTYTPSDPTVITICLLDVSGLIKQLLWVESLQDWETIYIQPKASCDVFAVCGPFSVCSDSALSLCNCMKGFSVKSPVDWELDDRRGGCIRNTPLEHCGSNRSNLTVLTDQFFPLPSVTLPYHAHSMETIESRQECMQVCLRNCSCNAYSYGKSGCSIWHGQLINVKQYNNGTVNINEEILFLRLAAGEVQSLGNNTKGGRRRLIIGLTVGAFGLLVMLLIISMGKRKWCGHPLNNTEDACGVIAFKYVDLQHATKNFSEKLGGGGFGSVFKGILRDSTAIAVKMLDGARQGEKQFRAEVSTIGMIQHVNLVKLIGFCCEGDRRMLVYEHMANRSLDAHIFQGNGTMLDWSTRYRIAIGVAKGLSYLHESCHDCIIHCDMKPENILLDVSLVPKIADFGMAKLMGRNFSRVLTTMRGTVGYLAPEWISGVAITEKVDIYSYGMVLLEIISGRRNSSSKECTSNSDHDVYFPVQAARTLLEGDVGSLVDERLHGDINIEELERACKAACWCIQDHDFDRPTMGDVVQVLEGLVDLDMPPMPRLLQAISGSSSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WND5</accession>
    <name>A0A8I6WND5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123438681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A8I6WND5"/>
    <dbReference type="SMR" id="A0A8I6WND5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000470.1">
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    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0000890.1">
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0000470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0000470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123438681"/>
    <dbReference type="OMA" id="MAARITY"/>
    <dbReference type="OrthoDB" id="661283at2759"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0000470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="373" mass="40778" checksum="73F049BC38817444" modified="2022-05-25" version="1">MAAVVAVESLKPEASTMSGQPVELSPGQLHKLAELIHRQEVQKLQELKFESYAEQQNYLKDAREARDKVYHILDSAQEMVTEAEADNDATKQEIAKDVYEYCTKAIGTSLKFIRSYNTRLTYLDKLKTHSDDLIKQLKWLDPVTQQKEAQRLALEAGMYKKAALENAEKFQHFIPNQFSKWLKDNKIKFEDLVTDNMAKLGFKGPFKNLGDIQKLQVYDNIIAAAGHGKPSVTYTFEALGKVGVAALVFTAAVMVWDIYTAEDKLQAAVRDAVNALTSVVDLVIGQVVNTAVRAGFAALNVQIASAAVTVIGSVVGFGIGVLVGVAAGALLDLIFGSGTSNVQITDGLSGCRVAPMPDGLELARLIKHNYPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6WNY7_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNY7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005440.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005440.1">
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005440.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="121" mass="14199" checksum="0CB2F97B18242B70" modified="2022-05-25" version="1">MKDKQSDIDANELFVELKFLQNFMPKENIGPVEILNFLKRHDCFPNASIAYRVLLTIPVTAEKSFCKLKLLKSYMRTTMTQQRLNDLATIALESEVLEKIDYEDIIEDFISKNTARMRMFK</sequence>
</entry>
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    <accession>A0A8I6WQF6</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0179210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
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    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="319" mass="36370" checksum="1A4489DD498B8FD7" modified="2022-05-25" version="1">MQVLFYCSETYDDSIENEKSLQNARIIKEFWNDYYAKLLDIDKTKKTSNVDMYQDDVRKLLINEKKNKNGVFSDTELIDLQNQIANCTMKFDEDNLFKVTPNIVKFLINKDQPNAKQYLKGCLPSSLPMLKMFGIYTLEAIMIHVLGLVFNTLQESSAVKAARFIDQLNSTVREQARFLQYKAPGSGKVEAVLTSKVESVKDVVQPKGASKKEKKKKIQCHYDIGKYLLQFMIERNVLHISTDRGVTKEDPVLVLKKGQGYIENSCYVMCNLNINLLPIKLNLPMLCKPLDWQPAERGSDPDTLSDLIGGYLCKPTGDI</sequence>
</entry>
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            <fullName evidence="1">LRAT domain-containing protein</fullName>
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        <name evidence="2" type="primary">LOC123446297</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978908.1">
        <property type="nucleotide sequence ID" value="XM_045122973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSM2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0075070.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0075070.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0075070"/>
    </dbReference>
    <dbReference type="GeneID" id="123446297"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0060710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0060710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0060710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0075070.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0075070.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0075070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446297"/>
    <dbReference type="OMA" id="NNGFRCY"/>
    <dbReference type="OrthoDB" id="421951at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137">
        <property type="entry name" value="OS05G0310600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137:SF4">
        <property type="entry name" value="PROTEIN LEAD-SENSITIVE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LRAT" evidence="1">
        <location>
            <begin position="20"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0075070.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="275" mass="29137" checksum="B2E364DC867C421C" modified="2022-05-25" version="1">MGLLSNRIGKESLKAGDHIYSWRAAWVYAHHGIYVGDDKVIHFTRGRDQEVGTGTVIDFLLVSSGPNRSSTPCLVCSSDEVTAATETNGVTSSCLSCFLSGGALYRFEYDVNPALFLAKVRGGTCTLAATDSDEVVVRRAKYLLSNGFRCYSLFKNNCEDFAIYCKTGLLVAEGNVGLGQSGQAVSIIGGPLAAVVSTPFRLVTTNIYGMAVMAVGVYCVSRYAGDIGNRRDVLKVEVEELTAGLASGRIRAANITQLATPGQVQAPAVTTLVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WST1</accession>
    <name>A0A8I6WST1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123430197</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970022.1">
        <property type="nucleotide sequence ID" value="XM_045114087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WST1"/>
    <dbReference type="SMR" id="A0A8I6WST1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0161630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0161630"/>
    </dbReference>
    <dbReference type="GeneID" id="123430197"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0133330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0133330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0133330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0161630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0161630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0161630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430197"/>
    <dbReference type="OrthoDB" id="1886792at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="140"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="205"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="348" mass="37457" checksum="5558347F3B59C004" modified="2022-05-25" version="1">MEHHQRLLHLSPHQHQEHLMIGQGFFDVDPMHFHGEDGGFAVEPAAAGVDDGAWMEDLMHLGDELFGGGGGGGGGVAGDENDMVGAAAADQAWRQECEGASPDDNPCSYDDVISPVSGEQGPGCEPSRDDSDLSGTRKRRDRSKTIVSERKRRFRMKEKLYELRALVPNITKMDKASIIADAVAYVKNLQSHARKLKEDVATLEARPGLAGRRQQQQQQQKQGRRQGQHGRNGGDDEGNSGSSRGGGGARVTLVSAAQVGEGRFFVTVECERRDGVAAPLCAAVESLAGFLVESSNLGCSSDRVVSTLTLKVSEATEEVMISDRTVKLWVMAALLSEGFRPEATSEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUD4</accession>
    <name>A0A8I6WUD4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Purple acid phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123430504</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044970305.1">
        <property type="nucleotide sequence ID" value="XM_045114370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUD4"/>
    <dbReference type="SMR" id="A0A8I6WUD4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180720"/>
    </dbReference>
    <dbReference type="GeneID" id="123430504"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0149410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0149410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0149410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430504"/>
    <dbReference type="OMA" id="HTYDSTK"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07378">
        <property type="entry name" value="MPP_ACP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000027">
        <property type="entry name" value="Purple acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024927">
        <property type="entry name" value="Acid_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051558">
        <property type="entry name" value="Metallophosphoesterase_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161:SF19">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000898">
        <property type="entry name" value="Acid_Ptase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035286580" description="Purple acid phosphatase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="13">
        <location>
            <begin position="35"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="35153" checksum="28562660E568D358" modified="2022-05-25" version="1">MAVLAILVSALVLLSPVAAELQRVQHSPKEDGSLTVLAVGDWGRGGQYNQTLVAEQMGVIGEKLAADFVISTGDNFYNDGLAGDNDTAFFKASFTDVYTASSLQKPWYIVLGNHDYTGDALAQQSPAIREVDGRWTSVDKSFIVEADIVDFFLVDTSPFVLKYWNESKFDWRNVAPRDTYIATLLQDLDDALTASNATWKVVVGHHPISSGCEHGNTTELREHLLPVLKAHGVDMYLNGHDHCLQRISSIDSPVEFVTSGGGSKAWAGKFKATSDKMEFLYDGQGFLSMQLTAAEAHLAFYDVSGAVLHSWGLAKSAAAASIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUI9</accession>
    <name>A0A8I6WUI9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123441182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973623.1">
        <property type="nucleotide sequence ID" value="XM_045117688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062110"/>
    </dbReference>
    <dbReference type="GeneID" id="123441182"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441182"/>
    <dbReference type="OrthoDB" id="2019753at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47880">
        <property type="entry name" value="OS05G0353300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47880:SF1">
        <property type="entry name" value="OS05G0353300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="51700" checksum="DF876079B365D332" modified="2022-05-25" version="1">MALSSRAAAFPHVSLPSPRPRPAAVSLRPPPPPRSVRLDHVIPADLGLAGGGGLLAAAIEHLEREPAAAADDKAPLADLSPRELQLVLVYFAQEGRDAYCALEVFDWLRRADRVDGETMELMTAIACGWIERLVGAGGDVSDVSALLGEMDCVGLRPGFSLVEKAVALYWDRSERARAVEFVRDVLRRGSVAAGGEYDGERGGPVGYLAWKIMMDGDYRDAVNLVIEFKESGLKPEVYSYLIGLTALVKEQKEFSKALRKLNLSVKDGSIAKLDAESMRNIEKYQSKLLGDGVLLSNWAVQEGSSEVLGLVHERLLSLYTCAGCGLEAEHQLWELKLVGREPDTQLYDVVLAICASQGETAAVRRLLAGVESTSAGRRKKSMSWLLRGYLKGGFYLDASATLMWMLDMGLCPDYLDRAAVLAALQRKIQEPGNLESYMKLCKRLSETDLIAPCVVYLYVRKFKLWMMRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYI3</accession>
    <name>A0A8I6WYI3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYI3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0122800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0122800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0122800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0122800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0122800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="154"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0122800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23354" checksum="CC13EFC443E1AF1F" modified="2022-05-25" version="1">MSLPCSDQSIRPKKMKSASLPRGVEAVRCWCGDLCKVKEVTDFSDWLGMKFFMCANYESDPPESISAYIRPPSPPPLCMYYRWIDTEMPDWAVTEIRERDRRAWASWDLEERREKAEAEERAAQKKEWEKYCVEQRAFLDDMIRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEASDGKGKYPRRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYN3</accession>
    <name>A0A8I6WYN3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123428099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968201.1">
        <property type="nucleotide sequence ID" value="XM_045112266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYN3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0203500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0203500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0203500"/>
    </dbReference>
    <dbReference type="GeneID" id="123428099"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0169270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0169270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0169270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0203500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0203500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0203500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428099"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015245">
        <property type="term" value="F:fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF37">
        <property type="entry name" value="PROTEIN FATTY ACID EXPORT 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0203500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="206" mass="20196" checksum="0350A42C05805C50" modified="2022-05-25" version="1">MALLATTPLLAPLAFSPAPISASQSSLLFLRAPAPALLSLRSASPGARLLAAVASKEPELGGGGSEGGDGAGSGGGGGGGSDPQGGGDEEEEEKMGDGLSMSQKLTLAYAALVGAGGVMGYMKSGSQKSLAAGGISALVLFFVHTQLPVRPVFASAIGLGISAALLSVMGSRFKKSGKIFPAGVVSLLSLVMVGGYAHGIMRSSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0H2</accession>
    <name>A0A8I6X0H2_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123443390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975673.1">
        <property type="nucleotide sequence ID" value="XM_045119738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0H2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253290"/>
    </dbReference>
    <dbReference type="GeneID" id="123443390"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0210270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0210270.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0210270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0253290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443390"/>
    <dbReference type="OMA" id="FFDTPFH"/>
    <dbReference type="OrthoDB" id="591619at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="377" mass="42250" checksum="E4DFDC22710E8338" modified="2022-05-25" version="1">MAYNGEPPVPVPVPVVVANVDHHPAPLIGLFFDSPLQHVFHSGLPCTPAFAPAHRSNPDLVAAILSGDFFLTTLQARDHQQALWSVLDARGGYLLLRGGGTLLALLNPLARRCERIFDLRDTHIFDDDDYLEGDLGLAYGGLICDDENPTSFRIFCLASHGEFRLRAAVFFSFLGMGGNWFLSPWLDVPHHPHGLSLDNGMRVGNFIYWPYRNRSYVVVLDPDPNNPRLFVEMIPPLLNLNGFLSSVLGQINGGNMCIAYSNGFNIGFMLRQEYGLDAGAWANIRVLNLTDPINAKFPPLPENRLEIAAMRGSILYLTTSEMFHGPHVLSWFACLCLESGRLEWLFARMYDGFFQPYHHSSWSTSLLPECERFDPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X190</accession>
    <name>A0A8I6X190_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X190"/>
    <dbReference type="SMR" id="A0A8I6X190"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0029320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0029320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0029320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF12">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="124"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="230"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="361"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="396"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="431"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="466"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="538"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="622"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0029320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="781" mass="86279" checksum="CF581148A8958CAB" modified="2022-05-25" version="1">MRRPSPPAAAAAAAESIDALALALRFSTSAAAAAVPPPPDLNPPRLDPSDVRSHNAALIAYSSRGSRAPRLAHLLASFGAMLKSGAPPNRCTYNTLINAHCKLALLADARAALVRMREAGLAPDTFTYNCLMLGLCRAGLLAAACGLFVQMPRRWGACYDRYSYTILIKGLCAAGRIDDACRVFAKMSRGWCRPGVHTYTVLLDGLCKVRRVGEAEALLGEMVNKGVVPNVVTYNALIGGLCQEGRFDDVTKLLEKMEIQRHSPDCWTYTQVVHGLCKHGKLMKRNRVNPGVQTFNEVIHGFCCGGKVHKAMAFLTQMAGAGLSPNTVTFNSLISGQCSVGERKIALRLLDLMEEYGVLPDRQTYAIFIDALCDEGQLEEAHSLFSCLPMKGIKAHNVIYTSLVHGYCQVGDIDSAFGLMEKMASENCMPDVHTYNTLIDGLCKVKRLDRAIDLLDKMKKQGIEPTTCTFNILIKQMLWDKKHADAAKMYEQMISSGCKPDKQTYTLKISTDWFEGATKEENIDMAVVEMHEAGVFPDVETYNAIIKAYVDAGLKEKAFFAHVKMLSVPIDPDCTTYSILLNYMCNKDDSDAFDNEKIWKMVDVRNLQELFEQMCESDAAPGISTYKALLRGLCNQCRLEEVEWLLLKMQGNSILLDEDMSDYLLGCYCNLEMYREACEQFRSMAHQSFQPGLKSCCLLLSGLCDSGDHGMAVSIFSDMLGLGYNYDEVVWKLLIDCLHEKGHAGACLEMLSVMDAKKCVASTRTYASLVRLVAELNEDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1M9</accession>
    <name>A0A8I6X1M9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 24">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8E5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0171670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26313" checksum="7FB7FC5ACBD1778A" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQCICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQTTVEMLENRDKDARALCMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSHARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAH5</accession>
    <name>A0A8I6XAH5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123421076</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044963429.1">
        <property type="nucleotide sequence ID" value="XM_045107494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAH5"/>
    <dbReference type="SMR" id="A0A8I6XAH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093130"/>
    </dbReference>
    <dbReference type="GeneID" id="123421076"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0076680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0076680.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0076680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0093130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123421076"/>
    <dbReference type="OrthoDB" id="598235at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1182">
        <property type="entry name" value="OS07G0186500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035324181" evidence="7">
        <location>
            <begin position="19"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="181"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="436"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="553"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="899" mass="102866" checksum="782D5D3E4682632F" modified="2022-05-25" version="1">MAEAVILVLLQKLASTLGGATLKVFGSRLGKEAIFLLEVENNMKEIEIEFDVMQAFINQVDPYLTNNQIFQCWLKHIRKVAFEVEDIIDEYAFLLGKMDGTESFLRKTFRQSKNVMIWYNVASQLKQVKSRLQNLTTMKERYGIKIYDNDETSSSHSISRKFYFSDSSYLNDHDNAIVGQKDHLKKLLGCLNDAGPDRAIIMIQGMGGSGKSTLARSIYRKQDVTLCHAWISTSRNRQVEDILTNIIDKLDIGSDEHDNSDCEGMVKTIQTYLQNRKYLIVLDDLWDRESWSCFDNAFPKNSHGSIVIITTRNEAVASLAEEEHTIRLGNLSEEESWDLFSKKAFMKLPEATCPDGLISCAKKILEKCQGLPLAIAAIGSLLSYRGMEEREWKSFYNQLNWQLTYNPELSRVSNVLSSSLNDLPTHLKNCFLYCGLFPEDCEIRRKWIIRMWIAEGFVEDRGAEITLEEVAEEYLKELIQRSLIQVVERNEFGRPRRFKVHNIVKEITRTSSKKQRFGLICYNPVLTSLGDTERRVFVHAGGQDFQPGAAWQQLRSFLLFDKHVSVPWIRAASSSFRLLRVMCLRYSLLQNFPNAIVGLFNLYYLDLSRTKVKKIPKSVARLKNLQTLHLRRTFVRKLPREIKLLINLRHLSVSSDLYGTSILGNICGLKSLQTLRDVKASKNLVQNLSFLMQLKTLSITNVLASQNKDLWSSVGKLTVLTRLAVASDADDEILSIKNFRAPQYLEKFYLDGKLADGALFPISGRFQNLKRLSMRLSGLVQDPVSALSEMTNLIYLELTCAYNGESLVFCCGSFLKLRQLRLEKLENLNSVEINNGSMVNLTDIEFHDLGKLEYVPEGLTYLTKLQHLVADKMPELFTRKLEEEHRNGFLQHIGSIECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAH9</accession>
    <name>A0A8I6XAH9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAH9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0457860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0457860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0457860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0457860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0457860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0457860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0457860.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26499" checksum="F4CEB4E31EB2030E" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDDAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDMDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRNHLPAHVDWLAIGGVTPLRGPLLPPVRGPRPHPCPYGPQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCU0</accession>
    <name>A0A8I6XCU0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123430861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970632.1">
        <property type="nucleotide sequence ID" value="XM_045114697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCU0"/>
    <dbReference type="SMR" id="A0A8I6XCU0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197370"/>
    </dbReference>
    <dbReference type="GeneID" id="123430861"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0163870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0163870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0163870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0197370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430861"/>
    <dbReference type="OrthoDB" id="1879545at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000033">
        <property type="entry name" value="S-norcoclaurine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF139">
        <property type="entry name" value="BET V I_MAJOR LATEX PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="3">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="165" mass="18355" checksum="2D3961CF681BB04A" modified="2022-05-25" version="1">MKGSLCHEFETGLPAAEVWEMYRGLRISQVVPQLLPGIFRKVELVEGDGGVGTVLHLTFSHGIAPLEYQKEKFIKIDHENYVKEVIVVEGGLLDHGFQKYLIRIEIIGQTKKTSTIRSSIEYEVDDEKTDNASFASTGALANLAEAITEYVKAQKSAEQAREETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEW1</accession>
    <name>A0A8I6XEW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965207.1">
        <property type="nucleotide sequence ID" value="XM_045109272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEW1"/>
    <dbReference type="SMR" id="A0A8I6XEW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0131580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0131580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0131580"/>
    </dbReference>
    <dbReference type="GeneID" id="123425581"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0131580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0131580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0131580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425581"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000092">
        <property type="entry name" value="Constitutive photomorphogenesis protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="53"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0131580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="20427" checksum="25B576BDC64F4891" modified="2022-05-25" version="1">MSSSSSFPGSRFPFAAGGAGAGASGGGAGGGGGGGSGSSVRPWGSSGGTSVSSSGKRIQKELLDLNASDCSAGPKGDNLYHWLSTIIGPQGSPYEGGIFFLDIVFPLDYPFKPPMVTFKTRIYHCNVDSIGAVSLEILKDGWSPALTISKVLLAIKGIITNPDPYNPLVESIARLYLTDRAKHDEIAAEWTLRFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGG4</accession>
    <name>A0A8I6XGG4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectate lyase</fullName>
            <ecNumber evidence="3 8">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123440320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="3 8"/>
    <dbReference type="RefSeq" id="XP_044972830.1">
        <property type="nucleotide sequence ID" value="XM_045116895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGG4"/>
    <dbReference type="SMR" id="A0A8I6XGG4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292910"/>
    </dbReference>
    <dbReference type="GeneID" id="123440320"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0243580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0243580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0243580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0292910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292910"/>
    </dbReference>
    <dbReference type="OMA" id="QGDKMQW"/>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00824"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
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    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
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    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF74">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342294" description="Pectate lyase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="9">
        <location>
            <begin position="105"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="378" mass="41031" checksum="F366FF9CE7759340" modified="2022-05-25" version="1">MAPKGLLLPVTLLLLLLGAATANLRHENIIDRCWRGEGNWATDRQRLAMCSVGFAGKMRQNRGHGVTAYTVTDPSDDPVRPRPGTLRYGATVLPGKVWITFQPGSMHIRLAQPLFVKSFTAIDGRGADVHVAGGAGIVLYQVSNVVIHGLHVHDVRAQPPGRVVRPGGAVKNLDAGDGDAIRLLSSSKVWIDHNTLSRCEDGLLDVTLGSTDVTVSNNWFHNHDKVMLLGHDDQHVADRRMRVTVAFNRFGPNVNQRMPRIRHGYAHVVNNFYDGWKDYAIGGSMGPSVKSQGNLFVASTPDSAKVTRRMPVGDAAGKDWHWHSTGDSFENGAVFAQTGSRVPPNYNRHQEFQAASSGEVRSLTKDAGALRCSAGAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJM7</accession>
    <name>A0A8I6XJM7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123446481</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the HIPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJM7"/>
    <dbReference type="SMR" id="A0A8I6XJM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0375600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0375600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0375600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0312620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0312620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0312620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0375600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0375600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0375600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45868:SF82">
        <property type="entry name" value="HEAVY METAL TRANSPORT_DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45868">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="6">
        <location>
            <begin position="62"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="119"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="355"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0375600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="393" mass="43010" checksum="ABDA453BB40240F8" modified="2022-05-25" version="1">MWSYSAPASTYKVEHRICCLPCLSSQRLESHRLERLLPKRDKVISPQGALPSHPMASGQPAVQTFVLRVTIHCHGCKKKVRKVLKSIEGVHDVKVDAQQHKVMVTGTVDAETLVKRLHKSGKQALPWQHTPAKNPEPAPSPSTPTDAPAPAEDGSNDAAVADKKPAVPAKEPQTSSSDKKPEQETSPEKKPEMDKEAEPEKKAEKEEAKPSDESKKAGGEGAAAEPKAKGTEPAETTKEAGNNEGEGKKKQSKPKDADRSLPPAPAHVPRHPHHEFNPYNAPQPVMSYNMAQPRASVSHYAPQPEQGYSQPQAGYSSMTQHSPAQQAYSQHAQPMQQWSPSYLYMPYPHASPESYYQDYYSPPGTNASPPPPPPLHDSYSLFDDENPNSCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLU1</accession>
    <name>A0A8I6XLU1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123450021</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983353.1">
        <property type="nucleotide sequence ID" value="XM_045127418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLU1"/>
    <dbReference type="SMR" id="A0A8I6XLU1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416730"/>
    </dbReference>
    <dbReference type="GeneID" id="123450021"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0347440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0347440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0347440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450021"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000057">
        <property type="entry name" value="kinesin-like protein KIN-14R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF18">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-14R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="435"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="995"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="275"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="778"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1031"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1087"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1115"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="518"/>
            <end position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1136" mass="125162" checksum="6FC6FFC9957DEF82" modified="2022-05-25" version="1">MEAGDDPMDFTWTAGWEAAHPACDSSPDPAPDPAPACDSSPDPKPAPPACDSSLDPAPDPAPAPQEAEAEPMILVSGPRVAVSGLRRADCHAGECVLFLNAGGCAIEDDDPSAKFSSDSFFQGGESIETSESILEGGDYPSLYSSARYGDFSYRFDGLAPGDYYLDLHFAEIVHTGGPKGIRSFDVLVQEDKILSELDVFAVVGGNRPLQVLDIRAYVGSNGVIIINFKGVRGNPMVCGICVRKAPATPAAKLGTRGTGLCKKCLTVVEISSPIQKRTIKLISKYEKQIEDLTSQCTSKSDECSMAWSLVESTNQELDRLKMELHQKLVQSDNFEQVLDTQTDQLRKVSQNYENDKKLWAAAISNLESKIKAMKQEQALLSLEAHDCAQAVPDLSKMIEAVRALVAQCDDLKVKYHEEMAKRKKLHNIVQETKGNIRVFCRCRPLSKVETSSGFKCVVDFDGANDGDIGIMNGGTTKKTFKFDRVYTPKDDQAEVYADASPLVTSVLDGYNVCIFAYGQTGTGKTFTMEGTERNRGVNYRTLEELFKIAEERKDTVTYNISVSVLEVYNEQIRDLLATSPSSKKLEIKQAGEGSHHVPGIVEAKVEDINEVWDALQTGSNSRAVGSNNVNEHSSRSHCMLCIMVRAKNLINGDCTRSKLWLVDLAGSERLAKTDAQGDRLKEAQNINRSLSALGDVISALASRSSHIPYRNSKLTHLLQDSLGGDSKALMFVQISPSDNDASETLSSLNFASRVRGIELGPAKKQVDTAELQKFKQMLERSKQEVKLKDDSLRKLEENCQNLEKQTKGKEQLYKNLQEKVKELESQLDSKEKQQNQLSEKLKEKEETCTALEQKIRELERNLEQQHRSDCDVAALNQSIEELEQLWLKLKEQEQQTSDAESKIEELELKLKEQEQQRSAAESKAREMEQELLEAQKTESMLESQLLDLKKKLPEMGARKLQDANAMPEPCNNPIPRVLPATPVEANMVPPPCAREVMSEKAQQQHHRILRSSDSANKRVPSSLFAEAGVVNEKKRKGEDSHPAASARQNAGRKRSSLQAEVENQLPAAAAATRKRSLQGGEVRSKRASNPARPSTSASSRATTASVAANKAVSGSRVTRQQQATVVSSNKTKGWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNE0</accession>
    <name>A0A8I6XNE0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pirin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123395069</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="4">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044945934.1">
        <property type="nucleotide sequence ID" value="XM_045089999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNE0"/>
    <dbReference type="SMR" id="A0A8I6XNE0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533220"/>
    </dbReference>
    <dbReference type="GeneID" id="123395069"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0443530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0443530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0443530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0533220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533220"/>
    </dbReference>
    <dbReference type="OMA" id="CTIFATT"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000055">
        <property type="entry name" value="pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="6">
        <location>
            <begin position="32"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="7">
        <location>
            <begin position="179"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32201" checksum="EF68FEE83F697766" modified="2022-05-25" version="1">MASSSSSITFENPRKVVKKVMSLSQSEGDGATVRRSIGGHEVRNLDPFLLLDEFSVSKPAGFPDHPHRGFETVTYMLDGAFTHQDFSGHKGTIRTGDVQWMTAGRGIVHSEMPAADGVHKGLQLWINLASKDKMIEPRYQELESKDVSQAEKDGVAVRIIAGEAFGVRSPVYTRTPTMYMDFTMQPGSQLHQPIPEGWNAFVYIIEGEGVFGKEGTAPASAHNCLVLGAGDGLSVWNRSGAPLRFVLAAGQPLKESVVQQGPFVMNSRAQIQKAMEDYYYGRNGFEKASQWSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS41</accession>
    <name>A0A8I6XS41_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123428427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    </reference>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="867" mass="97444" checksum="F0F93EBB16CEF9FB" modified="2022-05-25" version="1">MRVAVVGGGVSGLAAAHELLAASGGGDVRVTLYEQEESLGGRARTVAVDDGAGRLQLDLGFMSFNQVTYPHMMERLEGLGVEMERSNMSFSVSTQLKGGGGGCEWGNDNGISGLLARKTNILKPSFWRMVYDIIKFKNDALTYLEDHEQNPDLDRNQTLGQFTQSHGYSLSFQEAYLIPVCTGMWSCSPQDVLSLSAFFVLSFCRNNGLLQLFRHTQLPTVKPRSQSYVNKVKGELESMGCLIKTSCRVKSVSSLYDGAAGYRVLENDGSEETYDSVILGVHAPNALKVLGAEATHHELRILGACQYVQRDIYLHCDKNLMPRNSSAWSAWNFLGTTSRGFSVTYWLNQIQKIKSVMPFLVTLNPPCVPNHVLLKWNTSLLVPSMAGAKAYLQLDQIQGKRGIWFCGAYQGHGCHEDGLQAGRAAAQGLLGKKCELLLNSKQMIPSWTEAGARLVVARFFNLYVSIGNLIFVEEGGNVFSFGKACDKCRIKSVIRVHDPLFYWKVATEGNLGLAEAYINGSFSFLDKREGLLNLLLILIANRDERRNRRVARKGGRWTPLHVIAKLAHAKYILREVSRKNTVTQTRRNISQHYDLNNDFFSLFLDKSMTYSCAVFKVENESLEAAQQRKLSLLINKAKVKRGHHVLDIGCGWGSLAIQAVKQTGCKYTGVTLSAQQHKYAERIVREAGLEDHITFLLCDYRQIPPCKYGAIISCGMIEHVGHEYMDEFFACCESYLAEDGILVLQFISAPEERYEQYRRRTDFIKEYIFPGGCLPSLARVMSAMTTSSRFSIEHVENIGPHYYTTLMRWRDNFMINKDEILALGFDDKFMRIWEYYLIFSASCFKSRTLGDYQVVFSRPGNRRLAVP</sequence>
</entry>
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    <accession>A0A8I6XUX7</accession>
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        <recommendedName>
            <fullName evidence="4">WRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUX7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529390.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529390"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0440370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0440370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0529390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34680">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34680:SF9">
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    </dbReference>
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        <location>
            <begin position="161"/>
            <end position="205"/>
        </location>
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        <location>
            <begin position="14"/>
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            <begin position="25"/>
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            <end position="248"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="336" mass="38046" checksum="233F48D0606AF0C1" modified="2022-05-25" version="1">MRIRRAASLLLGATPSTFSSSQPPRLNPPPLPPAHVGGPPPCLTNMAVTDLMDQLGIEDPEDDKHFMETYFWDGIPSLLSHHPWGSRCPFPTTTYADFIEEKEEMVVDMIDDVILEPRIMENNSEENKIPTKKGEEEKNKTKSTTKGKGKEKLEVYESDDARDRWPCKMNESKRAFCGRTASQPNSYCLHHSDQKPCAISKPPHRKRIVANKGLDQGFCYYDGFGPSRSTKRHHGSSRRQEHVEQKEHAPPDYIDLTAGVAGADNIEHQVMSVSECVDEPRQDNYTDVIVNDFCEEISDDDDAPGFKCETIKVSKTLSKKRGKKHMKDRSLKSLMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVA9</accession>
    <name>A0A8I6XVA9_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVA9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0500990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0500990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0500990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0415830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0415830.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0415830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0500990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0500990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0500990"/>
    </dbReference>
    <dbReference type="OMA" id="GEEDQCC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35167:SF6">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35167">
        <property type="entry name" value="OS05G0216466 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0500990.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="139" mass="15148" checksum="C2C18EF056FC08F4" modified="2022-05-25" version="1">MATQPRQSSKKILQPEAQPGKRKMEPKPKPKTKKAGVAAPAGAEAFTALELDVAEQLIRLSESGVSSGGSFSCLRSVDNLPAPPVKGCCVDWEEDEDHEVAGRQRRVKRYRLINEIYAATATEQIGGCNGSNRKNQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVV7</accession>
    <name>A0A8I6XVV7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123395451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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                <person name="Muehlbauer G.J."/>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="EnsemblPlants"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        <location>
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            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="412" mass="44996" checksum="403AB640F9FB4D12" modified="2022-05-25" version="1">MDPGAAPAPPIPPGSAAPDAADSDADATLPVDLLLEIAARSDAVSVVRCAAAAKPLRRGILDRGFRRLLAQRATATSGYDPSLLRGVSYKVEDSDANSPVRVVQPVPADEPSVLRGFNESLDFEPVASRDGLVVLRRLRPRPLFGQSIEEGPPGSVLRVCHSFTGAVSVLPSVSIRDYRKHALLSVEDNGRAFELLVADERLRIQIYSSREGRWGPVRAALPRRQSRPFHDSYPLVIGRTVHWLCNPEPLPPLRYLSGPEPYIVAVDADTMEASVIDLPRGCTSRMTASMSHRGLLLASSVDGRLKVVVSETQVISMWTMSPPVEGEPPRWVRQVLIDKQDWGVHSSVQFEGFGLRSGTVILYVGRVGLIRLNLATKEAVIVYHRSDTAYISQVCLHEIDLSSLLQTMKPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2K8</accession>
    <name>A0A8I6Y2K8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447284</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979809.1">
        <property type="nucleotide sequence ID" value="XM_045123874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2K8"/>
    <dbReference type="SMR" id="A0A8I6Y2K8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409220"/>
    </dbReference>
    <dbReference type="GeneID" id="123447284"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0340820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0340820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0340820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0409220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447284"/>
    <dbReference type="OMA" id="VCPKMAG"/>
    <dbReference type="OrthoDB" id="605210at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF54">
        <property type="entry name" value="OS10G0105900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="50020" checksum="B3335DD929436136" modified="2022-05-25" version="1">MATFTARRGEPALVRPARPTPTETKALSDLDDQWTLRFYESIVGFFRAAPGEAPPRPGKVARGVKAAVAAALVHYYPMAGRLRRLPGGNRLAVDCTGEGVAFVEASADVRLEDLGQPLVPPYPCVQEFLGDCGDTRDVLAKPLLFLQVTQLKCGGFVIGLHMCHCIADGFGILQFIKTIADFGSGELIPTTLPVWKRDIFTARMPPSVAHVYPAYKPFLHGLECSGDDVMLSTPPECMEVQYLFFGPNEIEILRSHVPEHLSKSTTTFELITAVMWRCRTLALGYESTQKVRVMFTLNTRGRSINGESAVPRGYYGNAHFSPMVEVTVEELAAKPLGHILELMRQVKLDTTKDRIKSMVDLMALWRERSPFGMDRTYEVSDTKWVGGNALQFGKAELVAAGTPHAGDFTSKLISYHTKCKSKDGQDSTVVSILLPKPAMEKFTKEMAFWLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5Q2</accession>
    <name>A0A8I6Y5Q2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123404034</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5Q2"/>
    <dbReference type="SMR" id="A0A8I6Y5Q2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045096">
        <property type="entry name" value="EDR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136">
        <property type="entry name" value="ENHANCED DISEASE RESISTANCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136:SF64">
        <property type="entry name" value="OS02G0102800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="67"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="6">
        <location>
            <begin position="253"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="205"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="467"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="768"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="783"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="814" mass="91574" checksum="38292D68DD6E3BB3" modified="2022-05-25" version="1">MLSSSASRREPAAPSSATAKTADLSKAAATFAMPRDPSPGAAAAANAKELQAKASALAAVREAAAAAVHHEGWMVRYGRRKIGRSFFHTRYFVLESKLLAYYKKKPKDSMVPLKSLLIDGNCRVEDRGLKTHHGQMIYVLCVYNKKEKEHQITMGAYDIEDALAWKKKIEQIIDQQQDSMADKNRKAFASMDFDTDLGGQFAFSDHDSAAEEEEERPTLIRRTTIGNGPPESIHDWTNEPDIGSNQNEPSQFSSKKNWRLLRCQNGLRIFEELLEVDYLARSCSRAMRAVGVVEATCEAIFGLVMSMDVTRYEWDCSFRYGSLVEEVDGHTAIIYHKLQLHWCPMLVWPRDLCYVRYWRRNDDGSYVVLFRSIEHPNCGRQRGYVRAFIESGGFKITPLKCRNGRPRTQVQHLMQIDLKGWFLNYSPSFQYHTLLQIQNCVAGLREYFSQTDECHIAPRIPVMEKMFDPSAEQKNPKPRAIDKIKPLDRDQKDSRNMSIIEEESDEDDDYQVPEANIEDDPNKPDSDAKHSEPPEKIDLSCFSGVLHRDPDEKTRNCWTVPDSTLFKVRSKNFPTDKSKIPAPSYLMELAAIDWFKDTKRMDNVGRQKGCVAQLAAEKGMHTFVANIQIPGSTHYSIVMYFVTNTMKKGSLLQRFFDGDDEFRNSRLKLIPAVPKGSWIVRQSVGSTPCLLGKAVDCSYIRAPGYLEVDVDIGSSAVANGVLGLVFGVVTTLVVDMAFLIQANTYEELPEQVIGAARLAHVEPAAAVVPDLENNNDSKDTNNDDTTTTTTTTTNNNNNNNNATSSEDDSSKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6Q0</accession>
    <name>A0A8I6Y6Q0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6Q0"/>
    <dbReference type="SMR" id="A0A8I6Y6Q0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0610340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0610340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0610340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000013">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF809">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="158" mass="16253" checksum="57C866BEC178D53A" modified="2022-05-25" version="1">MVSSTLDDSLYFIKLSGMTVTGKPLAVSSSEYSSLPTIIDSGTVITRLPTTVYDALSKAVAGAMKGTNHADACSILDTCFVGQASSLRVPAVSVAFSGGAALKLSAQNLLVDVDSSTTCLAFAPARSAAIIGNTQQQTFSIVYDVKSNRISFAAGGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y796</accession>
    <name>A0A8I6Y796_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y796"/>
    <dbReference type="SMR" id="A0A8I6Y796"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0635000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052439">
        <property type="entry name" value="F-box/Kelch-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122">
        <property type="entry name" value="GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122:SF25">
        <property type="entry name" value="REPEAT-CONTAINING F-BOX FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="62095" checksum="71B8EC352AE781EE" modified="2022-05-25" version="1">MKSRVGTTSLFPSQSSASALLSPLRSTSPMRPPDGSSSSSGPHGKHELQPEVVLYTASASASRRRGQTSADRCYALRALLRGYGLTMDERDVSTSRAHHSELKSLLAARGCAFSLPQLLVGGRPVGGPDHVRKLHQTGGLRHLLDAAPRPCRAFVCQACKRVGSAPCPKCSQARNKMLDHGVMEEEQEEERVVLFYPTQDVSMEGVEEQQAVVSWSNTIDVIGRELFIRCLHLVPRRDYGASIASLNREFNSVVRNGDIYRLRRKNGVAEHWLYLCCGNNNNPPEWDAYDPSTGRWIHVPKMPPSGSYFWDSLAVGTELLLFGETGVALRYSILTNSWTGLPDADAINTPRYLFGAASGGEKAYVAGGLDSSRTNALSSAEMYDSETHTWTPLPSMNRARYGCSGAFMDGKFYVIGGVSSTSSREPLTCGEEYDLNLRSWRVIDNMSQGLNETVDGAPPLIAVVNNELYGADYSENNDLKQYDKLDNKWITLGKLPVQSKNKDAFDMGFRACGDRLIVIGRPNNSTDEKVVELHSWIPDGQPPVWNFVATRPYRGNGILCAAMGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7K9</accession>
    <name>A0A8I6Y7K9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Laccase</fullName>
            <ecNumber evidence="5 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123446578</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Secreted</location>
            <location evidence="3 13">Extracellular space</location>
            <location evidence="3 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5 13"/>
    <dbReference type="RefSeq" id="XP_044979090.1">
        <property type="nucleotide sequence ID" value="XM_045123155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7K9"/>
    <dbReference type="SMR" id="A0A8I6Y7K9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383780"/>
    </dbReference>
    <dbReference type="GeneID" id="123446578"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0319180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0319180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0319180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0383780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446578"/>
    <dbReference type="OMA" id="DERCIVL"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF287">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11 13" id="KW-0186">Copper</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035340920" description="Laccase" evidence="13">
        <location>
            <begin position="33"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="41"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="167"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="440"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="594" mass="64944" checksum="91C4510A431C48FD" modified="2022-05-25" version="1">MEATRATRRLMRLCCCLSGVLLLSFLLPSALAEERFYEFVVQETVVKRLCQTNRIITVNGQFPGPTIEVHNGDTLAIRAVNMAQYNVTLHWHGLRQLRNGWADGPEFVTQCPIRPGSSYTYRYTIQEQEGTLWWHAHSSWLRATVHGALVILPKRGVPYPFPKPHKEFPVVLAEWWRRDPIAVLRQSMVTGAPPNVSDTILINGQPGDFLPCSSQETSIIPVVAGETNLLRIINAAMNSELFVSLAGHKMTVVAADAVYTKPFETTVVLLGPGQTTDVLVTAHAAPGRYYLGARVYASAQGVPFDNTTATAIFQYKDAAGCPPTGAVAVAGAGAKTGNPGRAGPAPMFPMLPANNDTNTATGFSNLIRSPRPVKVPGPVTQEVFTTIGFGLFNCRPGPFCQGPNNTRFGASMNNVSFQLPNTVSLLQAHYHRIPGVFTEDFPARPPVVFDYTSQNVPRALWQPVKGTRLYRVKYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYNPRRDEAKFNMVDPPSRNTIGVPVGGWAVVRFLADNPGVWLVHCHIDAHLTGGLAMALVVEDGKTELQTTMPPPVDLPICGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8E2</accession>
    <name>A0A8I6Y8E2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123401328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951048.1">
        <property type="nucleotide sequence ID" value="XM_045095113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y8E2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624180"/>
    </dbReference>
    <dbReference type="GeneID" id="123401328"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0517940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0517940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0517940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0624180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401328"/>
    <dbReference type="OrthoDB" id="589312at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="43"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="497" mass="56792" checksum="B33EFD7E5A580E22" modified="2022-05-25" version="1">MKNKNGRPRKSALNQAAAGNKNGRRKRNLKATRNEAAASNANEDRLSKLPNDLLLNILERLDTLDAIRTCILSRQMLKLPTMLSRLYLTFSLKGVLRTNHAVAYVTDNILSTRSPEVTISELKIRFLLLPNDSLIIGRSVARAMATQKVAAAEFEIVTKKRYSLCSSADLLQFGKQFNDLFGACPHAFAGLTSLWLQNMRFGELDIPNILSTCKLLEYLRLSHCDSGVHAVLQVEHDQLVELEVDYGKFQRVELISLPKLQRMTYDNWFSSQDPLYFGFVPQLSKLSLIKTRIRSDKTLELSQLLANVPNIDYLRLDFRSEKIWILPECPKLLKPVLNKLQHVNLDHLPEGCDLAWTMFILEAAPSLKELCITVWDHWCIMITNKELRKKYCFCEKADVKWKPYAPDFKHKNLAKLTIYGFQPDDNFMRYIRCVAEHAVNITEISLHDRKVCGRCGDLDPEIKDSVCPSRYPPTAEERKQITEALGLSSVAVVHFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCA5</accession>
    <name>A0A8I6YCA5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123408245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957332.1">
        <property type="nucleotide sequence ID" value="XM_045101397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCA5"/>
    <dbReference type="SMR" id="A0A8I6YCA5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656910"/>
    </dbReference>
    <dbReference type="GeneID" id="123408245"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545180.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0656910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408245"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
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        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="330"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
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    <feature type="repeat" description="PPR" evidence="4">
        <location>
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        </location>
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    <feature type="repeat" description="PPR" evidence="4">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="540"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="76100" checksum="E117601B5E20CBA9" modified="2022-05-25" version="1">MQMLSRHRHRHGRRLVHFVLKPPPFSTTNAASAPPPPPPPVCTPDAAPLVFSLRDHDPGALAPDDAIAALSSLAESDGSAAALAFFRRLAARPDARHLMRLYVTAATAFVARGSLPMAHEAMRRMVAAFAEAGRLPEAADMVFEMRSHGLPFCVETANWILTAGLHSRNFAYARKVFDGMVTRGGVCPDARSFRALVLGCCREGRVEEVEALLAAMWGQGFCLDNATCTVVVRTFCKKGRFRDVSGFFRRMLEMGTPPNVVNYTVWIDGLCKRGHVKQAFRVLEEMVGKGLKPNVYTHTSLINGLCKIGWTERAFRLFLKLIKSSSYKPNVHTYTVMIGGYCNEGKLARAEMLLGRMLEQGLTPNTNTYTTLISGHCKGGSFDRAFELMNKMTREGSLPNIYTYNAVIDGLLKKGKIEEAYKVLRRATTQGLELDKVTYTIFITEHCKQGHITYALDLFNRMIENGCHPDIETYTTLIATYCQQRQMEESQKLFDKCLAIELVPTTQTYTSMIAGYCKVGKSTSALRVFDTMVQNGCLADSITYGALISGLCKESRLEEARALYEGMLDKHLVPCEVTPVTLAFEYCRRERTSVALSILDRLDKRRQIHATNVLVRKLSAMGNVDDASLLLKRVLDGDSAVDRVTYTGFINSCYANNRHALASEISDKISKKPSSFQEKNATVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6YCG7_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC123410008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044958813.1">
        <property type="nucleotide sequence ID" value="XM_045102878.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6YCG7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658660.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF93">
        <property type="entry name" value="AP2_EREBP TRANSCRIPTION FACTOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="123"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="17782" checksum="A8F3980D727F4601" modified="2022-05-25" version="1">MARPQQRYRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARIMCGPRVRTNFPDNDAAPSSSFLSPALVAKLHRFNVACGPQAAQQGDKGASASSVGVEPRTSPTPFAGYTGNGASAPSAAAGWSGGFLEEQYVEQMIEELLDSNFSMEISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF11</accession>
    <name>A0A8I6YF11_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF11"/>
    <dbReference type="SMR" id="A0A8I6YF11"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0689210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0689210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0689210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000219">
        <property type="entry name" value="Putative retroelement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="10" id="KW-0229">DNA integration</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="17">
        <location>
            <begin position="214"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="18">
        <location>
            <begin position="632"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="16">
        <location>
            <begin position="940"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1013" mass="114210" checksum="1854DB82ADC0FD3D" modified="2022-05-25" version="1">MAGIKTAKTMLLPVTINGERLTALVDMGSMHNFLSGDAMRRLALQPAGSEKFSVTVANGDRLACQGVARQVPVLIGDEPFSIDCVGIDLGCYDFILGINFLSTLGPILWDLDVLSLIFWREGDRRVQWTGIDGSGAVTPQLQLMAAALDEAHPLLADLLQQHTDIFDEPQGLPPVRPCDHRIHLLPDTTPVAVRSYRYPQLQKDELKRQVAVMLAQGIIRISTSPFSTLVLLVRKPDGTWRFCIDYRALNALTSKDKFLIPVVDELLDELDGARFFTKLDLRSGYHQVCMHPDDIKKTAFRTHHGHFEFLVMPFGLSNASATFQALMNNVLSPYLRRFVLVFFYDILIYSASWAEHLQHVAIIFNELLAHRLHLKRSKCSFGTTSVAYLGHVISADGVAMEANKVAAVAAWPIPRSPRELRGFLGLAGYYRKYIRDFGLIVAPLTRLLRRDAFSWDEEATTAFEALQRALTTGRVLQMPDFDMPFMVDCDASGIGFGVVLHQGEGPLAFFSRPFAARHHKLAAYERELIGLVQAGLRQRLDTGELDAPWRLDEGLLLHGRRIFVPDHDDLRHQVVTLAHSAGHEGMQKTLHRLRVDFYIPGDGAMVCDWVRSCMTCQRNKTETLRPAGLLQPLDVPSQVWADISMDFIEGLPKVGGKSVILTVVDRFSKYAHFIALGHPYTAASVAWAFFDGIVRLHVFTSSIVSDRDPVFRGHVWRDLFRLASVKLRMSTAFHPQTDGQSEVVNKVIAMYLRCVTGDRPRAWVDWLAWVEYCYNTSYHSALRTTPFEVVYGRPPPTMLPYEPGTARSETAGDLLRTRDDILAQARQRLLQAQQMARKYYDAHHREAEFAVGDWVCLRLLHRTTQSLDLRSKRKLGARYVGPFRVLERVDTLAYRLELPAGSRLHDVFHVGLLKAYRGDPPAATPTLPPTSDGRLLPASAQVAKVLQAQQRRGVWHVLVQWTGLPKEEATWEKLDDFRQQFPDVQLKDELFQKAGRDVMTGTTYQRRRPNGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF44</accession>
    <name>A0A8I6YF44_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF44"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0613060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0613060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0613060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0508310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0508310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0508310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0613060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0613060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0613060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0613060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="128" mass="14551" checksum="95B2FF9F11FF0B12" modified="2022-05-25" version="1">MGGFPFSAGRHLLMMKSSRQREQSRCAPLLHTPGTPAIEEDLLFRGMEEGRRPRRTAAQIRFWSIFLRGLTVTAGIAGAVFLLVMRFVYQTQELQDTFTMILLLIISFVPAGFFWCLTHEDMQTPTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YG87</accession>
    <name>A0A8I6YG87_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123398931</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin D subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949307.1">
        <property type="nucleotide sequence ID" value="XM_045093372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YG87"/>
    <dbReference type="SMR" id="A0A8I6YG87"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0487790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0487790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0487790"/>
    </dbReference>
    <dbReference type="GeneID" id="123398931"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0404110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0404110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0404110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0487790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0487790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0487790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398931"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20543">
        <property type="entry name" value="CYCLIN_AtCycD-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000034">
        <property type="entry name" value="D2/4-type cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000040">
        <property type="entry name" value="D6-type cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="93"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="190"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="319"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0487790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="37659" checksum="08683B273A8AB31F" modified="2022-05-25" version="1">MAPSYEMAASILLCAEDSSSVFGFGDEEESSSSSAAGGSPYCGGELAVEFPLPSEECVARWVATEAEHMPREDYAERLRAGGVDLRVRVDAVDWIWKVHAHYGFGPVTACLALNYMDRFLSLYHIPEGKAWMTQLLSVACLSLAAKMEETSVPQSMDLQAGDARYVFEAKTIQRMELLVLSTLKWRMQAVTPFSYLDYFLHQLSGGSAPSRQAVRDAAELVLCISRGTNCLEFRPSEIAATVAAAVAGEEGACCTHVDKERVVSCHEAMIQATGATVPPPKTASLMMGRAYSPAMSAPRSPTGVLDLDAGYLSSCTSDGTSTATMAASSSPPSASSGFDSSPVSSKRRKISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGX3</accession>
    <name>A0A8I6YGX3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGX3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551190.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0458030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0458030.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0458030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0551190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="99" mass="10621" checksum="1ADB269F889EB306" modified="2022-05-25" version="1">MAVDRADKENLPPATAAAAARPHGVRSCKLKRLGRARRRVPLRDITNVFVAESAVAELQQALPQQPQEGSAAAELAVKNGPAGGAVLKPGRYLLRKEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIL4</accession>
    <name>A0A8I6YIL4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123412277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961161.1">
        <property type="nucleotide sequence ID" value="XM_045105226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIL4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0647930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647930"/>
    </dbReference>
    <dbReference type="GeneID" id="123412277"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0537730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0537730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0537730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0647930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0647930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412277"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="entry name" value="Prob_Methyltransferase"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44067:SF3">
        <property type="entry name" value="OS06G0138600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44067">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="40316" checksum="4B3054D981589FB0" modified="2022-05-25" version="1">MGSVSLKLPAPRRRHGGPLSCLCSPAPLNLLMLLSLLSTNLLAFFAFFAPSPLHDPATTAPFSSSSNLSAHVAAIALEIGGASSSPGARLPDGLPPELLLFLTPHALPLGRDARTGLTHMPASVAASCLRSPSALALLSAFMSYAPHSACPRNATLPRRLVSKGCEPLPRRRCLSRGPRAPLPASGMGLDHRRWVAPARGGHEFLVDDVLRLGASKIRIGFDVAGGAANFAARMRERGVTVVTSVLDVAGKPMNEFVASRGLFPLLLSPAHRFPFYDGVFDLVHVGTAALDEAGAPAMGQAATPEALEFFMFDVDRVLRAGGLLWIDSYLCQSEERRRVVAKLIERFGYKKLKWVVGEKAGGASTSTYLSAVLRKPARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJ88</accession>
    <name>A0A8I6YJ88_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cytokinin receptor related to bacterial two-component regulators. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJ88"/>
    <dbReference type="SMR" id="A0A8I6YJ88"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0614430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0614430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0614430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0740760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0740760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0740760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000061">
        <property type="entry name" value="Histidine kinase 5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Histidine kinase 5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Probable histidine kinase 1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
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    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
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        <property type="entry name" value="His_kinase_dom"/>
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        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
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        <property type="entry name" value="HISTIDINE KINASE 5"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RESPONSE_REGULATORY"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="371"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="12">
        <location>
            <begin position="821"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="521"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00002427"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
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            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0740760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="970" mass="109030" checksum="71B0B07920593C91" modified="2022-05-25" version="1">MGDEYRCDSDDEVAPPMWPNVGDAHQKQFKMEELGKDHDALNDVEVREQPARVDHHRLMEIASSEKGTSQMQYFFKHWQYTRANNARLLSEEVSLLSQQRKEIEEKKQQILEEQRFQDENYYAAKRQVPILDEVYKNEWRRPSKKTDDHSRNQELKIDAEYNTISYWKERAMQLEIALEASLERERALEEKLEADIKNLQSHTPVEEFSGMLKRADYFLHLVLQSAPIVIAHQDADLRYRFIFNHFPTLADEDVIGKTDHEILSGEGIDEMNNVKREVMAKGIPTKREFVFNTPLFGAKTFVTYIEPVFSKSGETIGVNYVAMDITDQVKTREKMADIRVREAIQKAKETELSRSLHITEETMRAKQMLATMSHEIRSPLSGVLSMAEILATTKLDKEQYQLLEVMLSSGDLVLQLINDILDLSKVESGAMKLEATTFRPREVVKHVLQTAAASLKKELTLEGFIGDDVPLEVIGDVLRIRQILTNLISNAVKFTHDGKVGIKLHLVDKQQAGCELENGQLHKRAHPASPVTTATENSAASPRNYDKDALRCSNREDVCQNGVVSNENFREYQEGEVVWLQCDVHDTGIGIPEKALPCLFKRYMQASTDHARKYGGTGLGLAICKQLVELMGGTLTVVSYEDEGSTFTFMIPCKIPAKEENSDDPDDEASARNNFTINDIEGSFVFKPKVRPSLLSSGVPIMNNTKFFGSNLMCYDDSTNVIENHKPFSNGFISMKDNSGKCAPAASQSNGPNVSSIEEEKNDCSMVFELNSQAERVSSSRADTASVSGAGIQEGRKACKGLEENSLNKKSKCSPSSSKAKILLVEDNRVNIMVAKSMLEPLGHGIDIVNNGLEAIRAVQHHQYDIILMDVHMPVMDGLQATKLIRHFENTGCWDASVKPEHNEMIANSAISSDCVQDKKGKRVPIIAMTANSFAESADECLAAGMDSYISKPMNFQKTKECLQRYLLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YK50</accession>
    <name>A0A8I6YK50_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123397120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045091791.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6YK50"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0498610.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123397120"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0413790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0498610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0498610.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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    </dbReference>
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            <end position="279"/>
        </location>
    </feature>
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        <location>
            <begin position="117"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0498610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="323" mass="34741" checksum="E7BAE65056735D1D" modified="2022-05-25" version="1">MAASSTRPLNPAAHPFVPVASAPAKLCSSAWSSLPVGFPIPTQCLRPPPSLPLPCGLSVSVPPLPWRPWVKVPPPLPCHVTVYCRSLSCPVTAYCIPPPLLPGKSCSITKTVDGAGDKSLKAEVENRPSPRSVLSPRSPASVSARAPKPRAAPRPMGSKPAFDPRSAKTSLMICNIPNNFSKRRMMAILDQHCADENDKLRRRGGGKFDMSEYDFLYVPIDFGKKYNKGYAFVNMTTASAARRLHAFLHGHSWAAAGSRKVCEIVHANIQGANALAKHFSGSRFPCGNGNEEFLPVRFGPPRNGPRPTAERVIGHAVVASARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLM8</accession>
    <name>A0A8I6YLM8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glabrous enhancer-binding protein-like DBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123411061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GeBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959918.1">
        <property type="nucleotide sequence ID" value="XM_045103983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLM8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0668950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0668950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0668950"/>
    </dbReference>
    <dbReference type="GeneID" id="123411061"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0555040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0555040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0555040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0668950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0668950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0668950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411061"/>
    <dbReference type="OrthoDB" id="679858at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053932">
        <property type="entry name" value="GeBP-like_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662">
        <property type="entry name" value="BNAANNG10740D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662:SF13">
        <property type="entry name" value="OS09G0287600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04504">
        <property type="entry name" value="GeBP-like_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glabrous enhancer-binding protein-like DBD" evidence="3">
        <location>
            <begin position="134"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0668950.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="322" mass="35479" checksum="AD8DC205B700C043" modified="2022-05-25" version="1">MGKKRGEKRSAPPPPPPAASSEETHSGAHESEDEETIAVSRTPAPKNPPPPPQKGKESEASDEDEEEEEEEPSHAAPTTAPKKDPPAARQSEDSDNSNDDEDGGSGSDKDAPPPKPSVKQQAKGPEVKVPRLAFHRVWSTNDEVRILKALAAHQKEHGVLPQPDALVEALAGKLDNRAYSSKELQGKVKSLKHRYVSAAKKYELPTKEHDRRLFDLSKTVWARDKSMARAATAPTAGATAKANNHEPKGFDEMCELYPYLAEEVRKLGAPGMFKREFGKMDDDKARLLDEKIKKQRVAQMKVELHRSDLAREVTKAVMDLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPZ8</accession>
    <name>A0A8I6YPZ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402326</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952163.1">
        <property type="nucleotide sequence ID" value="XM_045096228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPZ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541490"/>
    </dbReference>
    <dbReference type="GeneID" id="123402326"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0450300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0450300.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0450300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0541490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402326"/>
    <dbReference type="OrthoDB" id="638496at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
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    </dbReference>
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        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mterf"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="385" mass="41839" checksum="F2F4DB5C8DC5E359" modified="2022-05-25" version="1">MLRLRSSILAHLLSSSPAASPASPLHRLISAAAPAIPPNPGSFAVEEYLVSTCGLTQAQAVKASAKLSHLKSPAKPDAVLAFLAGLGLSAADVATLVARDPRFLCAGVERVLAPNVAALTGAGLSRSDIARVLSLGRPQFRHRAMASTLHYYLHLLGSLDNLLRALKYGSLFSSDVERVVKPNVALLHECGLGACDIAKLCLSSRWLLNTNPQRLQAMVVCAEGLGVPRGSRMFRQALHAVAFLSQDKIAAKLEFLKNTFRWSDAEVGTAVSKAPTVLELSKALLQRKSEFLISEVGLEPAYLAHRPVILNYSMDGRLRPRYCVVKFLKENALLDRDRDYYSAVVLTEKVFMEKYICPHKVAAPHLTEDYAAACRGEVPTRFIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQJ8</accession>
    <name>A0A8I6YQJ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123395608</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044946568.1">
        <property type="nucleotide sequence ID" value="XM_045090633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQJ8"/>
    <dbReference type="SMR" id="A0A8I6YQJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462130"/>
    </dbReference>
    <dbReference type="GeneID" id="123395608"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0382520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0382520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0382520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0462130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395608"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14520">
        <property type="entry name" value="DSP_DUSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000083">
        <property type="entry name" value="Dual specificity protein phosphatase 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="118"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000941-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="367" mass="40503" checksum="5E1A917D9E08D7C3" modified="2022-05-25" version="1">MSAASRDAPVPPAMPDLVRDHLYFGDINDAIAALTASLPDGTDITHVLSVVSSASISFFTDYRPGLCLPTEEVRRVVAGEDGTPSVVAPGRLVRVVERTGEGLRVTRMAVPLKDTEEENLLDHLEPCLDFIDEGRKAGSVLVHCFAGVSRSATIITAYLMRTEQKSLEEALQSLKEINESVCPNDGFLDQLKLFEEMGFKVDTSSPLYRRFRLKLLGQSYRVGEKIGNHVFEDDPGVTRQPNPTEELSGKGKTPKTAYRCKKCRRMVAAQDNVIGHTPGEGNSSFEWHDKRKGHTHNKEQQDCSSLYVEPLKWMTPVEDGALEGKLSCIHCGARLGYFNWSGIQCNCGSWITPAFQISKSKVDVSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQK2</accession>
    <name>A0A8I6YQK2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950941.1">
        <property type="nucleotide sequence ID" value="XM_045095006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQK2"/>
    <dbReference type="SMR" id="A0A8I6YQK2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546180"/>
    </dbReference>
    <dbReference type="GeneID" id="123401258"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0454020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0454020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0454020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0546180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401258"/>
    <dbReference type="OrthoDB" id="1900877at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471:SF98">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Remorin C-terminal" evidence="3">
        <location>
            <begin position="396"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="456"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="508" mass="55806" checksum="C2F2A44A3A660518" modified="2022-05-25" version="1">MDYERIEKPSFPTQGGGFSPKRLRAMLLGVERRRKDDGVEGRQQEEEEEEEYGAVPMASVRSDDDARRGGSVCEEYKDVDVVSTMSGSSSSLDTGSGHRSRDSHSMGSRFRVPEEDSCDSESVASNFEFHKERGASARSAPTAVVAPFSKPPPSKWDDAQKWIASPATNRPGRAGGAFPRKMEKTGFGGGRLPPTKVVLEAMEEIDTKRIDSSQEKREIGWQKAANWATPEPYPEVEPCAKSAPGAESTIIDSAVTLSCDSSTTLHSATACIPPPPTGRSVSMRDMGTEMTPIASQEPSRTGTPVRATSPDCSRPTTPRRTLGINAAGGVINRGECSNADLSEEELQMKTRREIMLLGTQLGKTSIAAWASKKEEEKDASLSLKTVSSDQSAQNATEIRAAAWEEAEKAKYLARFKREEIKIHAWEDHQKAKIEAEMRKIEVEVERMRARAQDKLMSKLASTRHTADEQRAAAESKRDQSAARTAEQAEHIRRTGRMPPSLGCWNWCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YT13</accession>
    <name>A0A8I6YT13_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YT13"/>
    <dbReference type="SMR" id="A0A8I6YT13"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0570910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0570910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0570910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0473290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0473290.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0473290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0570910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0570910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0570910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1052">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="244"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="406"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0570910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="731" mass="83539" checksum="7DE758FA2540F0AD" modified="2022-05-25" version="1">MVISVMGKSGVGKTKLVKERYERHSTKRYFDVKAWVTCAPNLNASNLMKLMLSRLIGGSIKCTSDDDIRTMLHKVLENKKYLLVIDAEVSNTEWNIMLDYLPTRNIKSRVVRITQATDLEPPPPSFGEEKFELHHFQEHNITVELFLATLLMNEKEKHCGDLVIEAVKTNHAKFIFDVTGGLPLAVVLLSGLLRTKEYPGEWVKVFKHLDGNSNERKRLDNILSMCFDDLPHDLKSCFLYFAGFPASTLVKARSLVSMWMAEGFLRPKEGKTMEKVGERYLHELIHRRLMNLPPLENAAPGDERVTVQTRVHDFLVLEAQEANFMEIHSGDDVPTLSTTRRLSLQNHMDKYAALAEPLPKLRSIMSNFEKESQGGVETKTDIKTKVGACIPFPHKAQARSNDVMQKLLQGSKFLRVIFLDGLEIGKKLPSEISNVKHLHYLGITSCSIDEIPSSVGNLTRLQTLDVRGTSVTRLPREFWRIQTLRHVFGSIILPRRVGNLEQLQTLQEVKPDDDSDVWDTKTFTCMKRLQSLYISNLPEKNAQVALAAIYEVKYLMLLCIQGKVISLDLFTFSNFARLQVMILNGKIEPPLKDSDPLYFRKLSTLTKLTLNKTKVSQYFINKLSVELPLLASLALLPESYEERCLAFTDGFQSLKELKLDLDKCVEEIMIVDPACPCLEKLEITNYSKGLDLKVRHNIEKIIKHQDHYLYNKMKNMKVRDVDMVFHEDRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YTY7</accession>
    <name>A0A8I6YTY7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123400019</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950347.1">
        <property type="nucleotide sequence ID" value="XM_045094412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YTY7"/>
    <dbReference type="SMR" id="A0A8I6YTY7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497940"/>
    </dbReference>
    <dbReference type="GeneID" id="123400019"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0413150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0413150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0413150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123400019"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000229">
        <property type="entry name" value="Homeobox-leucine zipper protein HDG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042160">
        <property type="entry name" value="HD-Zip_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057993">
        <property type="entry name" value="HD-Zip_IV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN MERISTEM L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654:SF111">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ROC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25797">
        <property type="entry name" value="PDF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="121"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="14">
        <location>
            <begin position="324"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="123"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="71"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="776"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="853" mass="90945" checksum="C19C4FA60283E078" modified="2022-05-25" version="1">MSFGGMFDGAGSGVFSYDAGGGGAGMHNPGRLLAPPPIPRPGAGGGFASSTGLSLGMANMEGGGQLGDASRLGGYLGSTGSGGDGDSLGRAREDENDSRSGSDNLDGASGDDLDPDNSNPRKKKKRYHRHTPQQIQELEAVFKECPHPDEKQRMELSRRLNLESRQVKFWFQNRRTQMKTQIERHENALLRQENDKLRTENMTIREAMRSPTCGNCGGAAVLGEVSLEEQHLRIENSRLKDELDRVCALAGKFLGRPVSAISSPLSLPSSLCSGLDLAVGSNNGFMGMGMQSIPDLMGGGSAAMRLPAGMMGGGLDDGLGGEGVSIDRGALLELGLAAMEELVKVTQVDDPLWQPSLEIGIETLNYDEYRRAFARVLGPSPAGYVSEATREVGIAIINSVDLVNSLMNEARWSEMFPCVVARASTMEIISSGMGGTRSGSIQLMRAELQVLSPLVPIREVTFLRFCKQHADGLWAIVDVSVDGVLRPDSGAGGAGPAGYMGCRLLPSGCIVEDMQNGYAKVTWVVHAEYDEAAVHELYRPLLRSGQALGARRWLASLQRQCEYHAILCSNPHPNHGDRHEAISPAGRRCMLRLAQRMADNFCAGVCATAAQKWRRLDEWRVEGAGGREQASGGEDKVRMMARQSVGAPGEPPGVVLSATTSVRLPGTSPQRVFDYLRDEQRRGEWDILANGEAMQEMDHIAKGQHHGNAVSLLRPNATSGNQNNMLILQETCTDASGSLVVYAPVDVQSMHVVMGGGDSAYVSLLPSGFAILPDGHTMQAAPLDPSPQGSSPIAHGGGSNNNPGSLVTVAFQILVNNLPTAKLTVESVDTVSNLLSCTIQKIKSALQASIISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YU24</accession>
    <name>A0A8I6YU24_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YU24"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0499040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF87">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="401" mass="46506" checksum="8494A2CEB676DD98" modified="2022-05-25" version="1">MAVTLQDVSMITALPIEGKPLCMSTDFEGWRQQMEALIGMSPPEPEVEDGGKKDRVPAGAPFTWIAANFAHCPEDANDEVIQRYARVYMWYVISRTIFADGTGKNAPWMWLKALTVFDNKFTWGSAALATLYRQLDDACRRSTKDGGVGGCMLLLSVWSWERLPVGRPKTSKWNTWDDHGNPVRLPTWAYKWDLVSEVASEVNLLYKQYTNEMDSLTTEQVEWEPYGAWPNFGDAHPFELNPLCLQEKHLWLMCCPLICNWVVEFHLPHCVMHQFGLFQPHPPEWVDTDTQLHRLDRRRQRKIKDWHKHYKNYVNMFEQSESTRVEICPPAYEEDILEEPTEYDALAHGDYNKLIREGYQTSFAPVLNFVHKEVKKQADESEDILEKTPGGKKENLHFVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YWX9</accession>
    <name>A0A8I6YWX9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123405632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <end position="153"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="232" mass="25599" checksum="EA872721E8892385" modified="2022-05-25" version="1">MSQESDSISASTISTTATTGGCHLLKITGYSQSKQLPDGTNLRSCEFEAAGRSWCIEYVPRPPKKAEYVALALELITEGVGQPINTTVRFTLVPHHGKPEPATAPHYRSDRSRFHRTGVDRKGAYWSYPFITWEGLEGSGYLVDDCFSVRCDIHVIDTSAVADEVVQAHDLERMGLACSCTDDLCKRRHASSLALDLKKETPSDSASGSGSTTTSVQRRIKSIWFRLFGRKH</sequence>
</entry>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="6" key="1">
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="38939" checksum="9CDCB3E80F0940AF" modified="2022-05-25" version="1">MAASVWFFLWLASMGALQVSTVSIRLLANLVLCLRRPKDLRHTYGAWAVVTGPTSGIGRAMSLELARCGLNLVLVGRDPARLRDISQTISRTYAMQTKTVLFDFSLVSTAQGDKAMSRLREAVAGLNVGVLVNNAGVAKPGAVYLHEVDVEAWVRMIHVNVLALTEVTAEVLPGMLRRGRGAIVNIGSASGSVLPSHPLYSVYVATKRYVEEFSRSLSVEYKSTCIDVQCQIPFVVETNMVSSTVKNSFFVRPWVLSPDAYARAAVRWIGHGTLCIPNFAHCLQQWFTYLFPDSFKDMNLLKRNLQHRAIFRRIKPLREMRANSLDGRVPLSDKMGASVPLDQGGSDFRVHG</sequence>
</entry>
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    <accession>A0A8I6Z1W7</accession>
    <name>A0A8I6Z1W7_HORVV</name>
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        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1W7"/>
    <dbReference type="SMR" id="A0A8I6Z1W7"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0594240.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0594240.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
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    </dbReference>
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        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0594240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="22390" checksum="4FED33BA49506F70" modified="2022-05-25" version="1">MEFFTIILEKSSSTQVVPDNFVKMLDGHRPQNMKLRRAGNGLRKLWDVEVVFDMDGSMYLDRGWKQFVRAYDLRHGYFLVFRYDCNATNAVKVFDTTMCRRRYQDDNDASNGSRSSEYCDRCYVYGSSDSGYSKTSSDSGYSKSSSDDGLAMVIPQLGDRAMPIVVEEYIPQPGLGLRRSKRIRMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0L5</accession>
    <name>A0A8I7B0L5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <ecNumber evidence="13">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123425096</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + NAD(+) + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + NADP(+) + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DapB family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044964702.1">
        <property type="nucleotide sequence ID" value="XM_045108767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0L5"/>
    <dbReference type="SMR" id="A0A8I7B0L5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0021320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0021320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0021320"/>
    </dbReference>
    <dbReference type="GeneID" id="123425096"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0016770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0016770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0016770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0021320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0021320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0021320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425096"/>
    <dbReference type="OrthoDB" id="10259487at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02274">
        <property type="entry name" value="DHDPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000264">
        <property type="entry name" value="4-hydroxy-tetrahydrodipicolinate reductase 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000037">
        <property type="entry name" value="4-hydroxy-tetrahydrodipicolinate reductase 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011859">
        <property type="entry name" value="Dihydrodipicolinate_Rdtase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02130">
        <property type="entry name" value="dapB_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836:SF0">
        <property type="entry name" value="4-HYDROXY-TETRAHYDRODIPICOLINATE REDUCTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dihydrodipicolinate reductase N-terminal" evidence="17">
        <location>
            <begin position="70"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydrodipicolinate reductase C-terminal" evidence="18">
        <location>
            <begin position="196"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0021320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="342" mass="37090" checksum="C918CB5C6DBDA916" modified="2022-05-25" version="1">MLSAAFAVHPATVAPRRHSVRLRQQFCAPIGTATPRRRLPVAMLSITNAVASQSRESAPASAHRKLSFPILVNSCTGKMGKSVAEAAVAAGLQLVPVSFSAIEVPDGKLNICDRDIHIHDPSESESILRSIAKDYPDMIVVDYTVPDAVNANAELYCKLGLPFVMGTTGGDRQLLNKIVQDANVYAVISPQMGKQVVAFLAAMEIMAEKFPGAFAGYKLEVMESHQATKLDVSGTAKAVISCFQKLGVAFDLNEVNLVRDPEEQLAIVGVPEEHLGGHAFHNYHLTSPDETVSFEFQHNVCGRSIYAEGTVDAAMFLHTKIRSGADKKLYDMIDVLREGNMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B166</accession>
    <name>A0A8I7B166_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123441279</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973700.1">
        <property type="nucleotide sequence ID" value="XM_045117765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B166"/>
    <dbReference type="SMR" id="A0A8I7B166"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062390"/>
    </dbReference>
    <dbReference type="GeneID" id="123441279"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441279"/>
    <dbReference type="OrthoDB" id="550883at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241:SF32">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="141"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062390.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="415" mass="45028" checksum="3EB12BBF974547A6" modified="2022-05-25" version="1">MTVDRKGAQAAAAAAAATSAPFEIPALQPGRTCGAEESTRSHVLVEPIGSSDLLCNEYALLAQQNPKGDALPVASILRKKRPRRSRDGPNSVSETIKRWKEVNQQLEHDPQGAKRARKPPAKGSKKGCMQGKGGPENTQCGFRGVRQRTWGKWVAEIREPNRVSRLWLGTFPTAEVAAQAYDEAARAMYGPLARTNFPVQDAQAAPAVAVPVATEGVVRGSSASCESTTTSNHSDVASSSHNKQLQIQAPEISSRSDLLESTQSVEYSQQQSVPDAVSSIAMSTSEEDVYEPLEPISNLPDGEADCFDIEELLKLIEADPVEVDPVTVGSWNEFQDAGANARGSWNEFQCGVSWNANAGMEMGQQEPLYLDGLDQGMLEGMLHSDYPFPVWISEDRPMHNPAFHDAEMSEFFEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B312</accession>
    <name>A0A8I7B312_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123430213</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970033.1">
        <property type="nucleotide sequence ID" value="XM_045114098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B312"/>
    <dbReference type="SMR" id="A0A8I7B312"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0163240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0163240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0163240"/>
    </dbReference>
    <dbReference type="GeneID" id="123430213"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0134640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0134640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0134640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0163240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0163240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0163240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430213"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004825">
        <property type="entry name" value="PRK06181.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF91">
        <property type="entry name" value="OS04G0390700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
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        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="5">
        <location>
            <begin position="67"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0163240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="40070" checksum="4A539102CB998943" modified="2022-05-25" version="1">MEMLSMLKVGYTVLRSDTPATDLVNTFMDWAARRSLLLLALFVPPYHVYKLAASAAATVAPEDVAGKVVLITGASSGVGEQIAYQYARKGARLALVAGREGSLHEVAAKATDLGSPDVLVVPGDVARSEDCKAFVQATVERFGRLDHLVNNAGLTSVCWFEEMPDVASFKQILDVNFWGTVHPTHAALPHLKKSRGKIFVNSSAAAVLAMPRMSFCNASKAAVLSFAETLRMELGDEVGVTVATPGWIESEMTKGKHLSKGGRVEVDQDTRDARVGLFPVVRAERCAEAIVDAICRGRRSVTVPLWYRALFLWRSLAPEVGDVLQRVFYRRSSGHGGSQMRARRVLEVTGAKRLLQPPSLHTADIKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3B9</accession>
    <name>A0A8I7B3B9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Surfeit locus protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123427014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044966879.1">
        <property type="nucleotide sequence ID" value="XM_045110944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3B9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0175730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0175730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0175730"/>
    </dbReference>
    <dbReference type="GeneID" id="123427014"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0175730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0175730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0175730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427014"/>
    <dbReference type="OrthoDB" id="127285at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008833">
        <property type="entry name" value="Surf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47854">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2 (SURF2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47854:SF1">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2 (SURF2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05477">
        <property type="entry name" value="SURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0175730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="30732" checksum="16C2B00C16DD978F" modified="2022-05-25" version="1">MAKKGKATAAAASDSGASSPQGSVEGGGEKEGSFLLGQPTWEDAGGGRWRCAETGHELPEREKDAYARSRACRLALIDHGVAHKKPPLNAFKPHPEHKSKLICNITGDTINKSEEHIWKHVNGKRFLNKLEKLEEKMASGETGKIEGEQSNEVAKKSKSSKKKDKKKGKKEKKEAAVVNPSLPREPEPEIDDSDDSEFWVPPVGSRWDDDDGKDRWESSPGKDDIAEDEGASDDEDDDVMADKDDEVSEELASSRTKRLSVEAVGPSSFATRKKKPRKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B480</accession>
    <name>A0A8I7B480_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I7B480"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
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        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
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        <location>
            <begin position="9"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="14425" checksum="0704DBAD232EAD98" modified="2022-05-25" version="1">MDYEPAVFCSRCGRKASRWISWSVPNPGRRCYACVESKHGFLQWHDDPTSPFLRVLLGDLQDRVWSLEDSVAALCGDGDPAAGEVQERTVGAGAVQEAVHERTAGVGAVGDSVRMMVCGIVIFVSGLVVGMIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Triticodae</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I7B5E1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0126420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0126420.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0126420.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0126420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0126420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="241" mass="28184" checksum="6924A7200D583C23" modified="2022-05-25" version="1">MESYTLNEREHYEDVLGGPFLMELVHMMKLPIPEINVTKRKSGFWGVRTKVFGRKEDPKTEDINYGTLTHDCDMGMNTAVHDAIARLSYRHRAELGTHYFGRLGWQKPKGAHIILTHEQKVKFSPVVVYNQELEYYIKNLQMDLLSELFENDALHGSLKNDKLKVANVKKHDPEIQKLRQDLKNSQAMAHAGDVEIQKLRSQLLELQGRHRKLRINREYLAEELEKIKNDQKKPTDHEDMI</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="9" type="primary">LOC123440590</name>
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    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP-25 family.</text>
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    <dbReference type="RefSeq" id="XP_044973088.1">
        <property type="nucleotide sequence ID" value="XM_045117153.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8I7B666"/>
    <dbReference type="SMR" id="A0A8I7B666"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304730"/>
    </dbReference>
    <dbReference type="GeneID" id="123440590"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0254370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0254370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0304730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440590"/>
    <dbReference type="OrthoDB" id="19261at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15861">
        <property type="entry name" value="SNARE_SNAP25N_23N_29N_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNAP25 homologous protein SNAP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000040">
        <property type="entry name" value="SNAP25 homologous protein SNAP33"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044766">
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    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF34">
        <property type="entry name" value="OS02G0529500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="194"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="139"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="259" mass="28450" checksum="C66C7574CF298FD8" modified="2022-05-25" version="1">MPVAKGGKASSKPDPFDSDSDDGLVSSKPAKKSSAYSVPGGNKKQYQDGFKNSGGLENQSVEELEHYAAYKAEETTDTLNGCLRIAENIREDATSTMITLQKQGEQISRTHEKAVEIDQDLTKGEGLLNSLGGFFSKPWKPKKTKKIKGPVSRDDSFKKKVNRMEQRDKLGLSPRGKGNTREYGEAGTAMDKVQLEKKKQDDALDDLSGVLGQLQGMAVDMGSELDRQNKALDDMHGDVEELNSRVKQANQRARKIIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B775</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B775"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0377410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0377410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0377410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0377410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0377410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0377410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035326033" evidence="1">
        <location>
            <begin position="19"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0377410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="276" mass="31351" checksum="16CD50E6EF26E76C" modified="2022-05-25" version="1">MLPKFAFRALMLLHYSLLQMPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCSYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLSIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRMELYATRARLWTALTQLAPVVQTWYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYKMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAF1</accession>
    <name>A0A8I7BAF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC123394961</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="20"/>
    <dbReference type="RefSeq" id="XP_044945803.1">
        <property type="nucleotide sequence ID" value="XM_045089868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAF1"/>
    <dbReference type="SMR" id="A0A8I7BAF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530740"/>
    </dbReference>
    <dbReference type="GeneID" id="123394961"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0441490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394961"/>
    <dbReference type="OMA" id="QEPYMTM"/>
    <dbReference type="OrthoDB" id="1918782at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000384">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000016">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000025">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF9">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 24" id="KW-0732">Signal</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035202564" description="Receptor-like serine/threonine-protein kinase" evidence="24">
        <location>
            <begin position="28"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="26">
        <location>
            <begin position="21"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="499"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="783"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="839"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530740.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="864" mass="92148" checksum="AEB9DFB25767E7CF" modified="2022-05-25" version="1">MRPSWGDLAVVWCCFLLLLLLPFLSHGKDMPLGSTLTPGGNSAAWASPNSTFSLAFAPSPTSPSLFVAAVTYAGGISIWSAGAGAPVDSGGSLLLSSTGDLQLVNGSGAVLWSSGTAGRGVSAAALQESGSLVLKNSTGGAVWQSFDHPTDTVVMSQNFASGMNLTSGSYVFAVDRATGNLTLKWANAGSATVTYFNKGYNSTFTANRTLSSPTLTMQTNGIVSLTDGTLNAPVVVAYSSNYGESGDMLRFVRLDSDGNFRAYSAGRGSGTATEQWSAVADQCEVFGYCGNMGVCGYNGTSPVCGCPSRNFQLNDASNPRSGCRRKVELQNCPGNSTMLQLDNTQFLTYTPEITTEQFFVGITACRLNCLSGSSCVASTALSDGSGLCFLKVSNFVSAYQSASLPSTSFVKVCFPGDPNPPVSAGSTSSSRSSGLRGWVVALVVLGVVSGLVLAEWALWWVFCRNSPKYGPASAQYALLEYASGAPVQFSYRELQRSTKGFKEKLGAGGFGAVYRGVLANRTVVAVKQLEGIEQGEKQFRMEVATISSTHHLNLVRLIGFCSEGRHRLLVYEFMKNGSLDSFLFGAGSNSNDSGKAMSWATRFAVAVGTARGITYLHEECRDTIVHCDIKPENILLDEQHNAKVSDFGLAKLINPKDHRHRTLTSVRGTRGYLAPEWLANLPITVKSDVYSYGMVLLETVSGHRNFDISEETNRKKFSVWAYDEYEKGNILPIVDRRLAGEEVDMAQVERALQVSFWCIQEQPSQRPSMGKVVQMLEGIMELERPPPPKSSDSFMTVTTATTGGSASGVSSSVASTFASSVAAPPAPVPSPNVEQEMSVGRSASARTREIASLPLRSSEPYMTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAN6</accession>
    <name>A0A8I7BAN6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950027.1">
        <property type="nucleotide sequence ID" value="XM_045094092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537660"/>
    </dbReference>
    <dbReference type="GeneID" id="123399700"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0447370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0447370.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0447370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0537660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399700"/>
    <dbReference type="OrthoDB" id="1919377at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008390">
        <property type="entry name" value="AWPM-19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33294">
        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33294:SF5">
        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05512">
        <property type="entry name" value="AWPM-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="181" mass="19094" checksum="2B9727F1123A4032" modified="2022-05-25" version="1">MAGVGRNMVAPLLVLNLIMYIIVIGFASWNLNHFINGITNRPGVGGNGATFYFLVFAILAGVVGAASKLAGVHHVRTWRGDSLATSASSSLVAWAITALAFGLACKEIHVGGYRGWRLRVLEAFVIILAFTQLLYVLALHSGLFGNQFGNNNGGYPAEHAYGAGVGDHHNKGMDTGGVARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BE98</accession>
    <name>A0A8I7BE98_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123452498</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985090.1">
        <property type="nucleotide sequence ID" value="XM_045129155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BE98"/>
    <dbReference type="SMR" id="A0A8I7BE98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0467850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0467850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0467850"/>
    </dbReference>
    <dbReference type="GeneID" id="123452498"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0467850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0467850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0467850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452498"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF35">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="496"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="531"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="672"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0467850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="791" mass="87458" checksum="CED0F5B886DCF5D1" modified="2022-05-25" version="1">MRCAAAARSLLPLPPAAAPIHTHTASPSLGAELAAADALHALLSTLPPSLPTLLPCLSLLSPRLTTHTVSDALLWADLPVASRIRLFLFSALSPRLRSPLLHSRAVSVLLSVDPDAAMFDALADTRAAGLPAPSAAFAALITAHASAGRHQEAVDAFSRMGDFDCRPTASIYNAVFKVLVDSGAILLALALYNRMVSAGCPPNRATYNVLMDGLCKRGMAGDALKMFEEMLERGIVPDVKIYTVLISSMCSAGRTEEAARLICSMKEKGCPPDEITYNAFLSGLCKSGRVDDAFDRLELLRDGGYALGLKGYSCLIDGLFQAGRFDEGFSYYKEMLEKNVKPDVVLYTIIIRGFAEAGRTEDAFSLLNAMKDKGFVPDTFCYNTLLKAFCDVGNLDKAQLLQSEMLQNNVALDSTTHNIMICGLCKKGLVDEAMQVFHGMGELGCHPTVMTYNTLIDGLYRLGRLEDAQMLFYKMEMGNNPSLFLRLTLGANQVRDSESLRKLVDGMCESGQVLKAFKLVRGIIDSGVVPDVVTYNTLLNGLCKAKNLDGALRLFKELQLKGISPDDITYGTLIDGLWAAHRENDAMMLFQNILRGHGSPSLSIYNTMMRSLCRMKKLPQAINLWLDYLPKKYNLTPEDEVIANARKCFEVRSLDEAVKELIKIDQEYDSVNSNPYTIWLIGLCQVKRTDDALRIFHTLEECGIDVTPACCALLINYLCWDRNLNAAVEIMMYTLSKRFIVSQAVGNRLLRRLCIRYRRKDAQALAWRMHLVGYDMDVYLREPTKTLLYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGW6</accession>
    <name>A0A8I7BGW6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGW6"/>
    <dbReference type="SMR" id="A0A8I7BGW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0579510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579510.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF86">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="477"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579510.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1153" mass="131975" checksum="03547A812E6DF7FB" modified="2022-05-25" version="1">MSHLVWNYRGVGNRRTVREVRALVKAHSPSLVFLAETRQPSVKVEKMKWKLGLKGFCGVDSEGRGGDLALFWDESLHVTVLESCKRYIDVAVVDNGAGKSWRSTFVYGEPRVEHRQRMWDHLVRLKGTSALPWVVCGDFNEALWQHEHLSRTSRGESQMEAFRDTLMLCELVDLGFSGVPYTFDNGQNGQGNVRVRLDRACACDDWRELFPYVRVQHLVSPRSDHCPILLYLDASDDHRRRGSPKYEIMWERDPKLPDIISKAWARSRPGGDLGNVSASLKTVMADLRTWSKENFGQMEKQVATLQKELENLQLNDADRVLIRQKMQQLDELLYREEMMWLQRSRIVWLKEGDRNTKYFHRQAVWRVRRNNIRRLQKEDGSWCNSPSEMERMAASYFKEVYTKDPTLDPVAVLDVLEPKVSADTNEHLLVPYSDTEISDALFQIRPLKAPGPDGFPSRFYQRNWSCMKEEIIKAVRQFFVTGHMPEGVNDTCIVLIPKVQHPSSLKDFWPISLCNVIYKIVSKCMVNRLRPCLSELISENQSAFIRGRLISDNSIIAFECIHHIQSSPGSTDFCAYKLDLSKAYDRVDWDYLELALLKWGFAPRWVALVMECVRSVRYSVKFNGRLLQQFSPTRGIQQGDPLSPFLFLFVADSLSSLLHVAGQQRGLEAIKICRRAPAISHLLFADDSLLFFRASAESATIVKDVLNTYASVTSQLINPSKCSILFAESCSTQVAEDVKQILGVTQQAFEPKYLGLPVPEGCMHKGRFESLQSRLSKCLVDWSERYSSFASKEVLIKAVAQAIPVYVMSIFKLPLCVCDDLTKMMRQYWWGVENGKKKMSWIVWERMSLSKSQGGLGFRDMRAYNQSLLAKQAWRLLTVPDSLCAKLLRAKYFPNGNLVDTVFPASSSAVWKGIEYGLELVKQGMIWRVGNGANIRVWRDPWIPRGPPFTPILPKRNCRLNKVKDFLDGYGNWRIGLLQQYFCPADVDSIMKIRTSPRQLDDFVSWPWDRSGNLTVKSAYLFAMSTCWDQINPRASLSAPSGGRPLWKLVWNAAAPPKMQHFAWQVISGSLPTLAEKYRRHIGTTVVCALCGREETSFHALLVCPNAASLWDSMQRSWPLPNRTDIVCTGPEWLLHLLMESQELIRSRIIMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGZ5</accession>
    <name>A0A8I7BGZ5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGZ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0587910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0587910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0587910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0587910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0587910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0587910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0587910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="29099" checksum="8C52086D075D38A3" modified="2022-05-25" version="1">MPSRPSRQVVRLTRYIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYVCTIRVFPHYPGAEEPIHWSYGLGVTVDMAVQDAVYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTAEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPSHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJE3</accession>
    <name>A0A8I7BJE3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJE3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0705690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0705690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0705690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0705690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0705690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0705690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0705690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28985" checksum="8445C554D68F713E" modified="2022-05-25" version="1">MHGRPSRQVVRLTRCIDVPGHTVMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTAEMLENRDKDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRNHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2CPN4</accession>
    <name>F2CPN4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398115</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK353586">
        <property type="protein sequence ID" value="BAJ84805.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948552.1">
        <property type="nucleotide sequence ID" value="XM_045092617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CPN4"/>
    <dbReference type="SMR" id="F2CPN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441880"/>
    </dbReference>
    <dbReference type="GeneID" id="123398115"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0365830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0365830.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0365830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398115"/>
    <dbReference type="HOGENOM" id="CLU_083159_0_0_1"/>
    <dbReference type="OrthoDB" id="6379857at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043448">
        <property type="term" value="P:alkane catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000001">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050526">
        <property type="entry name" value="Rubredoxin_ET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47627">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47627:SF1">
        <property type="entry name" value="RUBREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="6">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ84805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="202" mass="21846" checksum="AE71BD0B5F4136F7" modified="2011-05-31" version="1">MAMATARLIHPCLVVSKSPRTAPTPLLLHTHKPLTSSFHFTLHSVDVSKDDKPLDTALETKQEDAAGVGVGDLTTPLPGELDAEEDVPKLDPRRFEEQFAVLNTGVHECRSCGYLYDQAKGDPSYPVPPGLPFAKVPDDWRCPTCGAAQSFFDSKSVEIAGFAQNQQFGLGGNSLTSGQKTLLIYGSLLVGFAFFLSGYFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXE1</accession>
    <name>F2CXE1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123444086</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK356294">
        <property type="protein sequence ID" value="BAJ87512.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976635.1">
        <property type="nucleotide sequence ID" value="XM_045120700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXE1"/>
    <dbReference type="SMR" id="F2CXE1"/>
    <dbReference type="PaxDb" id="4513-MLOC_80151.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279430"/>
    </dbReference>
    <dbReference type="GeneID" id="123444086"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444086"/>
    <dbReference type="eggNOG" id="KOG1533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CXE1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902182">
        <property type="term" value="P:shoot apical meristem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ87512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="304" mass="34356" checksum="FBD85F04E8460D7B" modified="2011-05-31" version="1">MVFGQVVIGPPGSGKTTYCNGMSQFLSLVGRKVAVINLDPANDALPYECAINIEDLIKLSDVMSEHSLGPNGGLVYCMDYLEMNIDWLEEKLKPFIEDHYFLFDFPGQVELFSLHTNARNIINKLVKKLDLRLTAVHLVDAHLCCDPGKYVSALLLSLSTMLHLELPHINVLSKIDLIENYGNLAFNLDFYTDVQDLSYLQYHLDQDPRSAKYRKLTKKLCDVIDDFSLVNFTTLDIQDKESVGNLVKLIDKSNGYIFSSIDSSAVEFSKIAAAPLDWDYYRTAAVQEKYMKDDEIIQKTSGMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2D183</accession>
    <name>F2D183_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123407603</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357640">
        <property type="protein sequence ID" value="BAJ88854.1"/>
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        <property type="nucleotide sequence ID" value="XM_045100770.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0023580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0023580.1"/>
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    <dbReference type="GeneID" id="123407603"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0018740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0018740.1"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0023580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0023580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0023580"/>
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    <dbReference type="KEGG" id="hvg:123407603"/>
    <dbReference type="HOGENOM" id="CLU_025359_1_0_1"/>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="8"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="183"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00892"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ88854.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0023580.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="384" mass="40964" checksum="EDFE16B10E00F79C" modified="2011-05-31" version="1">MSTDAGKKAYVVAMVIQVIYTGMYVVSKAAFDGGMNTFVFIFYRQAAATLLLLPLAILLERRNAPPMSLWLFTKIFMYALLGNTVSMNLHNISLKYTSATVASATSNSIPVITFLFAVLLHLEAIKFRAASGTAKLAGVALCVAGILVIALYAGPPLSPLNHHRAFHARAASTAGGKQGQWMKGTFLMLLANVTWSLWIVLQARLLKEYPNKLLATALQCLLSTVQSLALAAAVTAGSGEDMSAAWRLRLDVGLVAVAYSGFVVTGVSFYLQAWCIERRGPVFLAMSNPVGLVLTVFCSSFFLGEVVHLGSVLGGALLVAGLYSVLWGKSKEQPAPPPLSAGPASATTLKQGYVGSNVHTGAMNKEEDDAAKLEEKRTKLDSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2D6X2</accession>
    <name>F2D6X2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123444007</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediate ubiquitination of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
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        <property type="protein sequence ID" value="BAJ90843.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976538.1">
        <property type="nucleotide sequence ID" value="XM_045120603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D6X2"/>
    <dbReference type="SMR" id="F2D6X2"/>
    <dbReference type="FunCoup" id="F2D6X2">
        <property type="interactions" value="1789"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2D6X2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276900"/>
    </dbReference>
    <dbReference type="GeneID" id="123444007"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0229440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0229440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0229440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0276900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444007"/>
    <dbReference type="OMA" id="PNIASMY"/>
    <dbReference type="OrthoDB" id="1839788at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D6X2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000001">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 28, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="11">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ90843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="16564" checksum="19711FD411A97F60" modified="2011-05-31" version="1">MASKRIQKELKDLQKDPPTSCSAGPVGEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKDQWSPALTISKVLLSICSLLCDPNPDDPLVPEIAHMYKTDRHKYESTARTWTQRYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F2D9U6</accession>
    <name>F2D9U6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123398065</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
            <tissue evidence="13">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK360658">
        <property type="protein sequence ID" value="BAJ91867.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK370847">
        <property type="protein sequence ID" value="BAK02045.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948509.1">
        <property type="nucleotide sequence ID" value="XM_045092574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D9U6"/>
    <dbReference type="STRING" id="112509.F2D9U6"/>
    <dbReference type="PaxDb" id="4513-MLOC_37989.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456090"/>
    </dbReference>
    <dbReference type="GeneID" id="123398065"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0377470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0377470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0377470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0456090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398065"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031247_4_1_1"/>
    <dbReference type="OMA" id="CHINNNP"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D9U6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000055">
        <property type="entry name" value="Amino acid permease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="11">
        <location>
            <begin position="39"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ91867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK02045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="479" mass="51504" checksum="E22E277663CBB919" modified="2011-05-31" version="1">MAVGNGGAKKVVAPMEVSVEAGNGGDEAWMDDDGRPRRSGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPAVMLLFAAVIYYTSTLLAECYRTGDPATGKRNYTYMDAVRSNLGGPKVIFCGVIQYANLVGVAIGYTIASSISMRAIRRADCFHANGHADPCKSSSNPYMILFGLVQIVFSQIPDFDQIWWLSIVAAVMSFTYSGIGLSLGITQTISNGGIKGSLTGISIGVGITATQKVWRSLQAFGDIAFAYSFSNILIEIQDTIRAPPPSEAKVMKQATRLSVATTTVFYMLCGCMGYAAFGDAAPDNLLTGFGFYEPFWLLDIANVAIVVHLVGAYQVFCQPIFAFVERWAASTWPDSVFISREFRVGPFALSVFRLTWRSAFVCLTTVFAMLLPFFGNVVGLLGAVSFWPLTVYFPVEMYIRQRGVPGRSTQGICLRMLSVGCLIVSIAAAAGSIANVIEALKVYKPFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F2DF09</accession>
    <name>F2DF09_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123403005</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362476">
        <property type="protein sequence ID" value="BAJ93680.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952917.1">
        <property type="nucleotide sequence ID" value="XM_045096982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DF09"/>
    <dbReference type="SMR" id="F2DF09"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628130"/>
    </dbReference>
    <dbReference type="GeneID" id="123403005"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0521420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0521420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0521420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0628130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403005"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000219">
        <property type="entry name" value="Cinnamoyl-CoA reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF793">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ93680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="345" mass="38616" checksum="8C6F942C81D80A8C" modified="2011-05-31" version="1">MRSYSETKANGNREKEQQLVCVTGAGGFIGSWVVKVLLLRGYRVRGTARDPADNKNSYLLDLEGAKERLSVCRADLLDRDNLNGVFRGCNGVFHIASPMFNTDPELMVVAVEGTRNVIHAAADAGVRRVVFTSSYGAVHMDPNRNLDAVLDETCWSDYEFCKQTGNMYCCSKMMAEITATEEAAKRGLELSVVVPSMTIGPALQHKLRVVVPSTVHISRYLTGAKKTCPNVVTAYVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCEDDGKPMVKPYRFSNQRLRDLGLDFTPLKESLHETVLSLQQQGHLDLPVVPGPERARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F2DGU8</accession>
    <name>F2DGU8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123407106</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="SMR" id="F2DGU8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0679980.1">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SBP_sf"/>
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        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 6"/>
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    </dbReference>
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        <property type="entry name" value="SBP"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_SBP"/>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="11">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="162"/>
        </location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="38"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <end position="76"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
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            <begin position="142"/>
            <end position="153"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2DHA3</accession>
    <name>F2DHA3_HORVV</name>
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        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
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    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123412692</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AK363270">
        <property type="protein sequence ID" value="BAJ94474.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DHA3"/>
    <dbReference type="SMR" id="F2DHA3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0718900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000015">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF54">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="54"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ94474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="47203" checksum="88857A45F8E4F235" modified="2011-05-31" version="1">MAMSRAAAAVRQRRRVPLLPAARSMASWFGHVEPAPKDPILGVTEAFLADPSPDKVNVGVGAYRDDAGKPVVLECVREAERRIAGSTNMEYLPMGGSVKMIEESLKLAYGEDSEFIKDKRIAAVQALSGTGACRLFADFQKRFLPDSHIYIPTPTWSNHHNIWRDAQVPQSAFAYYHPESRGLDFAGLMDDIKKAPEGSFFLLHACAHNPTGVDPSEEQWREISQQFKVKNHFPFFDMAYQGFASGDPERDAKAIRIFLEDGHQIGCAQSYAKNMGLYGQRAGCLSILCDDEIQAVAVKSQLQQIARPMYSNPPLHGALIVSTILGDPALKSLWLKEVKGMADRIIGMRKALKDSLEKLGSPLSWEHITNQIGMFCYSGMTPEQVDRLTNEFHIYMTRNGRISMAGVTTGNVAYLANAIHEVTKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMF6</accession>
    <name>F2DMF6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zeta-carotene desaturase</fullName>
            <ecNumber evidence="10">1.3.5.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">9,9'-di-cis-zeta-carotene desaturase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123425989</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the conversion of zeta-carotene to lycopene via the intermediary of neurosporene. It carries out two consecutive desaturations (introduction of double bonds) at positions C-7 and C-7'.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>9,9'-di-cis-zeta-carotene + 2 a quinone = 7,7',9,9'-tetra-cis-lycopene + 2 a quinol</text>
            <dbReference type="Rhea" id="RHEA:30955"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:48716"/>
            <dbReference type="ChEBI" id="CHEBI:62466"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">Carotenoid biosynthesis; lycopene biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Plastid</location>
            <location evidence="10">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Plastid</location>
            <location evidence="10">Chromoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the zeta carotene desaturase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.6" evidence="10"/>
    <dbReference type="EMBL" id="AK365074">
        <property type="protein sequence ID" value="BAJ96277.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965700.1">
        <property type="nucleotide sequence ID" value="XM_045109765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMF6"/>
    <dbReference type="SMR" id="F2DMF6"/>
    <dbReference type="STRING" id="112509.F2DMF6"/>
    <dbReference type="PaxDb" id="4513-MLOC_22142.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0144000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0144000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0144000"/>
    </dbReference>
    <dbReference type="GeneID" id="123425989"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0118640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0118640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0118640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0144000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0144000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0144000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425989"/>
    <dbReference type="eggNOG" id="KOG0029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="LNMTWYS"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="UniPathway" id="UPA00803"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DMF6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009509">
        <property type="term" value="C:chromoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016719">
        <property type="term" value="F:9,9'-di-cis-zeta-carotene desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000111">
        <property type="entry name" value="Zeta-carotene desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014103">
        <property type="entry name" value="Zeta_caro_desat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02732">
        <property type="entry name" value="zeta_caro_desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF41">
        <property type="entry name" value="ZETA-CAROTENE DESATURASE, CHLOROPLASTIC_CHROMOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 10" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 10" id="KW-0957">Chromoplast</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="11">
        <location>
            <begin position="72"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ96277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0144000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="568" mass="62598" checksum="5EEF0AED5C0016E9" modified="2011-05-31" version="1">MAATSCALVSALVVGRRRGPSRRRAAPIGVVRCSLDSKVSDMAVNAPKGLFPPEPEHYRGPKLKVAIIGAGLAGMSTAVELLDQGHEVDLYDSRTFIGGKVGSFVDKQGNHIEMGLHVFFGCYSNLFRLMKKVGADNNLLVKEHTHTFVNKGGIVGELDFRFPVGAPLHGIQAFLRTNQLKVYDKARNAVALALSPVVQALVDPDGALQQVRDLDGVSFTDWFMSRGGTRESITRMWDPVAYALGFIDCDNISARCMLTIFTLFATKTEASLLRMLKGSPDVYLSGPIKKYITDRGGRFHLRWGCREVLYDKSLDGETYVKGLLISKATSSEIIKADAYVAACDVPGIKRLLPLEWREWDMFDNIYKLDGVPVVTVQLRYNGWVTEVQDLEKSRQLQKAVGLDNLLYTPDADFSCFSDLALSSPADYYIEGQGSLIQAVLTPGDPYMPLPNEEIISKVEKQVLDLFPSARGLEVTWSSVVKIGQSLYREAPGNDPFRPDQKTPVKNFFLSGSYTKQDYIDSMEGATLSGRRTAAYICGAGEELLAIRKKLVVDHVEKASGKVQMLQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F2DSY5</accession>
    <name>F2DSY5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123403188</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367003">
        <property type="protein sequence ID" value="BAJ98206.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953076.1">
        <property type="nucleotide sequence ID" value="XM_045097141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DSY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555690"/>
    </dbReference>
    <dbReference type="GeneID" id="123403188"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0555690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403188"/>
    <dbReference type="OrthoDB" id="582343at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141:SF112">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="67"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="162"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ98206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="524" mass="57506" checksum="064B7FAA63DE6B2F" modified="2011-05-31" version="1">MAGRAPYTNLDPVRAAGLQRMGFDPLELDRATEQLLTYVCTSLIPDFPFPATGRLVARPVSDGVDRISRLPRALLCNIVSRLPASDAARTAALSSRWRTIWLSTQPVLVDAYLRKDDYVWPPTPANSRAITAVVSRILEAHPGPFRCVHLACSHMNAYQAQLARWLQLLAAKGVQDLVLVNRPWPCDVILPDALFTINTLVRLYIGLWKLPDTARLGSYRTFPHLRELGICSVVMEQGDVDAIVARSPVLEVLSIQGSNKGLRLHLVSESLRCVQICGSVVEDIAMVKAPLLERLILHGCRDLAGGMCTRIRILDAPNLRVVGFLEPANHVLKIRDTIIRAGMKESTTTSLTSVKILSLNVRFGIRGEAEMVPRFLKCFPNVKTLHIMSGKCNQSTGTLNLKFKEVPMEISMSGISEMYFHEFRGDEGEVAFLKSLFKSAYGLEKAAIMMANPSLTPFSPDDALSKVKSELSISPGHVVVLVSGGPEGGRPWSFQKGTDFSCEDPFSVVAVSRRTAKFSATGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2E050</accession>
    <name>F2E050_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123442641</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369521">
        <property type="protein sequence ID" value="BAK00722.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974682.1">
        <property type="nucleotide sequence ID" value="XM_045118747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E050"/>
    <dbReference type="SMR" id="F2E050"/>
    <dbReference type="PaxDb" id="4513-MLOC_76498.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228960"/>
    </dbReference>
    <dbReference type="GeneID" id="123442641"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0189870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0189870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0189870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0228960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442641"/>
    <dbReference type="OMA" id="RWQPERL"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E050">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK00722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="218" mass="24123" checksum="CAB3E4C868FB41C1" modified="2011-05-31" version="1">MPVRVVDTATPSSQPSSGQDANAVQPSPPSCSLLTAGRCFAGTQNVSSLQKDEAWKVNVRIHGCDMEQGYLCGTMEALNVPLADTPVVTFWEGEIVDAKNYTFFTGKWEASAEDDVRHWSKFSSFTPLLSQIEADGGKSVDLSNYPFIFMRWKEQYFVNVGVDCGLTIAGFYYVCFSCSDGSISGFYYDPNSSPFQKLELKCTNEKQSGFTFSSYELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F2E276</accession>
    <name>F2E276_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123407276</name>
        <name evidence="14" type="synonym">PFK</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by AMP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="AK370247">
        <property type="protein sequence ID" value="BAK01448.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956313.1">
        <property type="nucleotide sequence ID" value="XM_045100378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E276"/>
    <dbReference type="SMR" id="F2E276"/>
    <dbReference type="STRING" id="112509.F2E276"/>
    <dbReference type="PaxDb" id="4513-MLOC_67234.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651530"/>
    </dbReference>
    <dbReference type="GeneID" id="123407276"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0651530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651530"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_7_2_1"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E276">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000002">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000018">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01981">
        <property type="entry name" value="Phosphofructokinase_II_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012004">
        <property type="entry name" value="PyroP-dep_PFK_TP0108"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005301">
        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000534">
        <property type="entry name" value="PPi_PFK_TP0108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="97"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="468"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="166"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="265"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity; cannot use PPi as phosphoryl donor" evidence="14">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK01448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="494" mass="53870" checksum="7A03BA56A9B07854" modified="2011-05-31" version="1">MDGAPPPKPEAKLAAGEAGYVLDDVPHVSDYLPDLPTYPNPLQDNTAYSVVKQYFVNPDDTVCQKIVVHKDGPRGNHFRRAGPRQRVYFEPDEVHACIVTCGGLCPGLNTVIREIVCGLSDMYGVTKILGIQGGYRGFYARNTIDLTPKSVNDIHKRGGTILGSSRGGHDTMKIVDSIQYRGINQVYVIGGDGSQRGAGVIFEEVRKRGLKVAVAGIPKTIDNDIPVIDKSFGFDSAVEEAQRAINAAHVEAGSAENGIGLVKLMGRYSGFIAHYATLASRDVDCCLIPESPFYLDGEGGLFKYIEKRLKENGHMVIVVAEGAGQKLIVENMKEMGQDASGNALLLDVGLWLSQKINEHFKKNKTTINLKYIDPTYMIRAIPSNASDNVYCTLLAHSVVHGAMAGYTGFTVGQVNGRHCYIPFYRITEKQNKVSITDRMWARLLSSTNQPSFLSKQDVDDAKVEDERTAKLLDGSPSNPKVEDKVATSNSNGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFD0</accession>
    <name>F2EFD0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123418299</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK374856">
        <property type="protein sequence ID" value="BAK06052.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962925.1">
        <property type="nucleotide sequence ID" value="XM_045106990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFD0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083860"/>
    </dbReference>
    <dbReference type="GeneID" id="123418299"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0068900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0068900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0068900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083860"/>
    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012438">
        <property type="entry name" value="DUF1639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33130:SF84">
        <property type="entry name" value="DUF1639 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33130">
        <property type="entry name" value="PUTATIVE (DUF1639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07797">
        <property type="entry name" value="DUF1639"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK06052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="223" mass="24924" checksum="CD130BCA593E4520" modified="2011-05-31" version="1">MAATADPRAKPPVAPSTHHLEPWARPQQPPRPHRVPAVESPSGAASGGRDRRRPSSESHRRDAAGDDDDDGIEGLRVKLMGHLRDAADRLRVPKKSPPLPPPPKAKEPEPDRMAPPPEQQPPQENVADKPWKLRERTRRRPAALSSSWPAAPPPPSRRRKRAPFSVALSTEEIEEDIYALTGGRPRRRPRKRPRAVQRQLDSLFPGLWLAEVTADDYKVPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL15</accession>
    <name>F2EL15_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123447961</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK376843">
        <property type="protein sequence ID" value="BAK08037.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="15780" checksum="110926108B86604B" modified="2013-04-03" version="1">MAGCGGKDGLYAVLGVSSDCSDAELRSAYRKLAMKWHPDKCGSSGGAEAAKARFQKIQAAYAVLSDPNKRILYDVGAYDSDGDDEGAGEILGDILEAMNQTPPQEDGESESLEDLHKQFEELFLKPDAYKSPPTPSSSSSKTRVSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0WJQ9</accession>
    <name>M0WJQ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123395279</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044946189.1">
        <property type="nucleotide sequence ID" value="XM_045090254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WJQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0464020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0464020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0464020"/>
    </dbReference>
    <dbReference type="GeneID" id="123395279"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0384060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0384060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0384060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0464020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0464020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0464020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395279"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WJQ9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16669">
        <property type="entry name" value="RING-H2_RNF181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF242">
        <property type="entry name" value="OS06G0633500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="331"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="376"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="407"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0464020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="506" mass="56514" checksum="F66D71000A870178" modified="2013-04-03" version="1">MSGNQTQSSDYERLASQPDAQCLVCTRPFTLDTEVTNSFEALAICRECKATVLNDSERDVTTSTSQQRRQRRRRSRTASLESNEDAFSQQFSQLINLARQGHEADIDSPTVVQQLASYNSTPNQSQRWHASDDESDGLSYADSVFDELESNISFGDYGGESDASLGQHSMMGREITIQLDNESYMNTDTDIDPMNAGIDQWDSDDQEDEQLEESDFDEAVDAMQQRQQQSRGIGPSELTGWESEDGVWTWRSQRANMTNLMADMEVPDVRTPFVGNPGDYADARQFEMILEQFAEDDSSRRGAPPAATSFIGNLPSLVISTSHEADGGLICPVCKDPMPIRTRAKQLPCMHLYHSSCILPWLSSRNTCPVCRYELPTDDPESERPERAAANERDVHGVEERTHLQEIGEEGSDEPEAEETQDMSYDAVEVTNTSEHGVHVAEQSNRARAHRRWLFIAVAPAVSFVGLALVLCFANPASSGRRRLCRGSQSASAVLVDSERSWWSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0X9T6</accession>
    <name>M0X9T6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0X9T6"/>
    <dbReference type="SMR" id="M0X9T6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0491670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491670"/>
    </dbReference>
    <dbReference type="OMA" id="DLETWAF"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0X9T6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF906">
        <property type="entry name" value="OS08G0205100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="253"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="620" mass="70550" checksum="5F615811AE2D2E8E" modified="2013-04-03" version="1">MVTTRLDEVAARACHNDRACIYIMKPLEEEDSRRLFFNRVFGSENVCSVQFKEISVEILKKCGGLPLAIITITSLLASHESGSSKEWESIKNSLGTKFATKPALEEMRGILNLSYTHLPIHLRPCFLYLGMYPEDRLIIRDDLVRQWIAEGFVCSLHGADLEDVAKSYFIELINRGLIQPEETCYGDVITCRLHDMMLDLILSKSTEDNFISVAYKYEDMTRFNSCEYKVRRLSLQSSVGGALSKTLATSMSQVRSYTQFGQSNYMLPLPQFKYLQVLAFEFPFNWKTIVDLRATGHLFLLRYLKVSALSAEVTLPAKIQGFVHLETLELHCRPIQRFPSDITRLPNLFHLILPECTLLPEGIQSMKSLRTLHCSDMSESSLKEIIGLSELTNLKELKLRVPTYFPGMTIECFDALLSSIGMLRDLKHLSLECWHKYDGYDSQPDSLSDPPPYLQTIHFEGMSFGVVPKWIGELRCLRILSLRVMYLPGDRVRALGKLPSLVFARFYVSDVSQDKVVVRTGLFPVLECFDFRSDEDVTANLSFEPGAMPKLRRLALAFDWQEWRGATPTGMEGLPCLQDIGLSVTTVSSNNNLKRIYEAMESAFKDAARVHPRHPSVTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>M0XFI8</accession>
    <name>M0XFI8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980695.1">
        <property type="nucleotide sequence ID" value="XM_045124760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XFI8"/>
    <dbReference type="SMR" id="M0XFI8"/>
    <dbReference type="FunCoup" id="M0XFI8">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XFI8"/>
    <dbReference type="PaxDb" id="4513-MLOC_59593.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0349850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0349850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0349850"/>
    </dbReference>
    <dbReference type="GeneID" id="123448004"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0291920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0291920.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0291920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0349850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0349850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0349850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448004"/>
    <dbReference type="eggNOG" id="ENOG502RSU3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XFI8"/>
    <dbReference type="OMA" id="EDCKGSV"/>
    <dbReference type="OrthoDB" id="646981at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XFI8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="399"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="211"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0349850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="474" mass="52148" checksum="1D4817269D30B389" modified="2013-04-03" version="1">METMSYPCSPLLSLPTHEESNFFIWSPQVALHENATIHVDHSTDQQHDNVFLDTAAQHYANDWQQQDASDVGVFTYCGERILGQESGNLVAIQEELMEENSLTDLLLTGAEAVEAGDPRFALAVFSRLDGLLLGTPENAAAGSFDRLAYHFSQGLRSRISSASTSCSPPEPLASDRMSVQQIIQELSPFAKFAHFTANQAILDATKGDAHVHVVDLNIGEGIQWPSLMSDLARHGGISFHLTAVTADADYSDAVHHASARWLSEFAESLNLPFQYSSLCLHSDEDLHEFSKNSKGSVIFSCDTTSMPYKLLSKLPALLPACVELLQPKLMVIIEEELVRIGKEVSLCKASFVDFFFEALHHFTTVFESLASCFAGGNQGVCLRLVEREMVGPRIQDFVGQYGSVTAEVNAPVVLERYGACDLSGCNVAQARMLVGLFNRGFEIVHEKGRLMLCWKSRPLTSVSVWAPILKERSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>M0XKD8</accession>
    <name>M0XKD8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0XKD8"/>
    <dbReference type="STRING" id="112509.M0XKD8"/>
    <dbReference type="PaxDb" id="4513-MLOC_60808.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0626380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0626380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0626380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0626380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0626380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0626380"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RRNI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XKD8"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0626380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="512" mass="58718" checksum="5BB30392EDF75A4B" modified="2013-04-03" version="1">MSSSLGRQAFARPTAKPSARGLNMHNRWSCLANLVEEEDELDRLSNLPDGLLLDILGRLDIADAARTRILARRWKEIPTMLSKFFITVGSFDDERNRELTCDNVVRANATVLGATRSLLESRTTSPFSINLLSVQFFLGDGSVSNIGQILANTMVTKKVGSVKLSLFTMKEGKQCRKDDVLTYGRQFKSFLDACPNTFSGLAQLKLENLRLAESGFREIFSICKQLEFLRLYNCDMGYLSLLEVSHPLLRELEIVKCDFERVDLMWLPKLTMLTYSWWVCEHDPLSFDYVPLLQTLSLTSMALARHKVLKLSEFLGKASISDLHLNFLCEKIWVKPEEPNQLLQVFHKLRHVTLTHISEVCDLNWTMFILQGAPSLQELCIKVWDHLCEIIEDEEQRKIYAFSKDKKDACAVWEAPPSDFKHHNLSVLRVFGFQCEERFVNYVKSVMEASVALGDIYLYEKPVCEDCEHVRQNDNYPRTSRLRTALRNIFDRGMCSLLRIHFPLSTTAGRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>M0ZCH9</accession>
    <name>M0ZCH9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0ZCH9"/>
    <dbReference type="PaxDb" id="4513-MLOC_81553.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0083420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0083420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0083420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0101130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101130"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SYYC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FFWATSH"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0ZCH9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF37">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="1">
        <location>
            <begin position="840"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1025" mass="116548" checksum="D2CB4BED576293E0" modified="2013-04-03" version="1">MPPEKIKLEAKNIDSAAEEIIGVLEDASKGNMIYFKGWGGFGASALLNLVAQRLKSLATLNKFAKVIHVDCSVWKNMRALQKAVGEELELPQSVMAIFERCDQEDDFNGIEEGSRGVIAEISREICRKLLSSKFVLIFHNGSRSYIDLYECGVPVISLLGNKVLWTWHVRFRYCAYLILEDGEKGKMKSQSDILLSASPNNVELPELLDLVRDLLHEDALEVAKCAGIVQPDDMSHKIVMECLIYRTLIAMDLKTHAPNYWVCDGIIQDQDNASAWEIGNSLERNMSSYWNFPYNEDTASVLQVSDDFLVNRWNFTTHEQLLKDYTRVLPPQVTSFFLSEDEPPTNTTSILPVRMFQYSQSSKLRVLHLSHCIFSFASPPFLCCSQLRLLHLDHCKNITHDHEHPSHSENMPCFQKLWVLDLRYTNWYWLLSEKMKRVMVELRELNVEGVKHGSISDLCGGRPSLVILRVTADPVPTEKKDTTIQAQFPNVFTSNSLTTSSFINDVLPPCLKSFTFINKAATVAKIFSISFRGSSLLKNILLRGNLGSLEELDLSGTAVKTLDLREVEAPILKRIILMSCGKLRAILWPPKDKGTWVLQKLHISTVQSASPNQNNWEEKTQEATTAAGSPSILTFGAQPQGIGQAASFQFNFYICVVDPRLMRSLVPFERHIVKHSVYMEIDSSPTATCGAAAGGRDVSQGMGSQQQVAQYLYARDVFQEHPQLVGAIEGVISWMWACPPIPIPSPQDWYFHMQDEEKMERGSLQRQRNTQRISIGVALAPVLMCDNARMLYVHDSSSTTCIPCPPDSDWNCLEWCRVERCPNLCSVFATPELKYRGTSFFSASYRHLVFLHIDCCPRLRHVLPLPASMNHYISPLPSLETLEIVCCSDLKEIFPLEPKRQENQQIVYFANLRRIHLYELPMLHHICGSRMFAPKLETVKIRGCWSLRILPAVSGDTSKRPKVDCEKEWWDNLEWLNWLGCYSDERKGCYSDQSWAEIKHDPSLYELSHSRYYKKADLPRGTVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-12-06" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q30H27</accession>
    <name>Q30H27_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">FT</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">Flowering locus T 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Flowering locus T1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Vrn-H3 late flowering allele</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FT1</name>
        <name evidence="4" type="synonym">FT</name>
        <name evidence="8" type="synonym">LOC123409685</name>
        <name evidence="3" type="synonym">VRN-H3</name>
        <name evidence="5" type="synonym">Vrn-H3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="Proc. Natl. Acad. Sci. U.S.A." volume="103" first="19581" last="19586">
            <title>The wheat and barley vernalization gene VRN3 is an ortholog of FT.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Fu D."/>
                <person name="Li C."/>
                <person name="Blechl A."/>
                <person name="Tranquilli G."/>
                <person name="Bonafede M."/>
                <person name="Sanchez A."/>
                <person name="Valarik M."/>
                <person name="Dubcovsky J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2006" name="Proc. Natl. Acad. Sci. U.S.A." volume="103" first="19581" last="19586">
            <title>The wheat and barley vernalization gene VRN3 is an orthologue of FT.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Fu D."/>
                <person name="Li C."/>
                <person name="Blechl A."/>
                <person name="Tranquilli G."/>
                <person name="Bonafede M."/>
                <person name="Sanchez A."/>
                <person name="Valarik M."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="17158798"/>
            <dbReference type="DOI" id="10.1073/pnas.0607142103"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="journal article" date="2006" name="Science" volume="0" first="0" last="0">
            <title>The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley.</title>
            <authorList>
                <person name="Turner A."/>
                <person name="Beales J."/>
                <person name="Faure S."/>
                <person name="Dunford R.P."/>
                <person name="Laurie D.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2006-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yan L."/>
                <person name="Fu D."/>
                <person name="Li C."/>
                <person name="Blech A."/>
                <person name="Tranquilli G."/>
                <person name="Bonafede M."/>
                <person name="Sanchez A."/>
                <person name="Valarik M."/>
                <person name="Yasuda S."/>
                <person name="Dubcovsky J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="5">
        <citation type="journal article" date="2008" name="J. Exp. Bot." volume="59" first="2707" last="2715">
            <title>Effects of photo and thermo cycles on flowering time in barley: a genetical phenomics approach.</title>
            <authorList>
                <person name="Karsai I."/>
                <person name="Szucs P."/>
                <person name="Koszegi B."/>
                <person name="Hayes P.M."/>
                <person name="Casas A."/>
                <person name="Bedo Z."/>
                <person name="Veisz O."/>
            </authorList>
            <dbReference type="PubMed" id="18550600"/>
            <dbReference type="DOI" id="10.1093/jxb/ern131"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="6">
        <citation type="journal article" date="2010" name="BMC Genomics" volume="11" first="707" last="707">
            <title>Genome-wide SNPs and re-sequencing of growth habit and inflorescence genes in barley: implications for association mapping in germplasm arrays varying in size and structure.</title>
            <authorList>
                <person name="Cuesta-Marcos A."/>
                <person name="Szucs P."/>
                <person name="Close T.J."/>
                <person name="Filichkin T."/>
                <person name="Muehlbauer G.J."/>
                <person name="Smith K.P."/>
                <person name="Hayes P.M."/>
            </authorList>
            <dbReference type="PubMed" id="21159198"/>
            <dbReference type="DOI" id="10.1186/1471-2164-11-707"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="7">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="8">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="9">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ100327">
        <property type="protein sequence ID" value="AAZ38709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DQ898517">
        <property type="protein sequence ID" value="ABI55200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DQ898520">
        <property type="protein sequence ID" value="ABI55203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EF055987">
        <property type="protein sequence ID" value="ABK91684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DQ900686">
        <property type="protein sequence ID" value="ABL11232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007825">
        <property type="protein sequence ID" value="ABV59387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007826">
        <property type="protein sequence ID" value="ABV59388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007828">
        <property type="protein sequence ID" value="ABV59390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007829">
        <property type="protein sequence ID" value="ABV59391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007830">
        <property type="protein sequence ID" value="ABV59392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007831">
        <property type="protein sequence ID" value="ABV59393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007832">
        <property type="protein sequence ID" value="ABV59394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007833">
        <property type="protein sequence ID" value="ABV59395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU007834">
        <property type="protein sequence ID" value="ABV59396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU331816">
        <property type="protein sequence ID" value="ACA29355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EU331872">
        <property type="protein sequence ID" value="ACA29356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q30H27"/>
    <dbReference type="SMR" id="Q30H27"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653910"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043994_6_1_1"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010229">
        <property type="term" value="P:inflorescence development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048510">
        <property type="term" value="P:regulation of timing of transition from vegetative to reproductive phase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000001">
        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF9">
        <property type="entry name" value="PROTEIN FLOWERING LOCUS T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ38709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABI55200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABK91684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABL11232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABV59387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACA29355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="177" mass="19851" checksum="F07B355A2501E7C6" modified="2005-12-06" version="1">MAGRDRDPLVVGRVVGDVLDPFVRTTNLRVTFGNRAVSNGCELKPSMVAQQPRVEVGGNEMRTFYTLVMVDPDAPSPSDPNLREYLHWLVTDIPGTTGASFGQEVMCYESPRPTMGIHRFVLVLFQQLGRQTVYAPGWRQNFNTRDFAELYNLGQPVAAVYFNCQREAGSGGRRMYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3T5P1</accession>
    <name>Q3T5P1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C-repeat binding factor 9</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="11 14">CBF9</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="13">CRT/DRE binding factor 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CBF9</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2005" name="Plant Mol. Biol." volume="59" first="533" last="551">
            <title>Structural, functional, and phylogenetic characterization of a large CBF gene family in barley.</title>
            <authorList>
                <person name="Skinner J.S."/>
                <person name="von Zitzewitz J."/>
                <person name="Szucs P."/>
                <person name="Marquez-Cedillo L."/>
                <person name="Filichkin T."/>
                <person name="Amundsen K."/>
                <person name="Stockinger E.J."/>
                <person name="Thomashow M.F."/>
                <person name="Chen T.H."/>
                <person name="Hayes P.M."/>
            </authorList>
            <dbReference type="PubMed" id="16244905"/>
            <dbReference type="DOI" id="10.1007/s11103-005-2498-2"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Rachis</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2006" name="Theor. Appl. Genet." volume="112" first="832" last="842">
            <title>Mapping of barley homologs to genes that regulate low temperature tolerance in Arabidopsis.</title>
            <authorList>
                <person name="Skinner J.S."/>
                <person name="Szucs P."/>
                <person name="von Zitzewitz J."/>
                <person name="Marquez-Cedillo L."/>
                <person name="Filichkin T."/>
                <person name="Stockinger E.J."/>
                <person name="Thomashow M.F."/>
                <person name="Chen T.H."/>
                <person name="Hayes P.M."/>
            </authorList>
            <dbReference type="PubMed" id="16365758"/>
            <dbReference type="DOI" id="10.1007/s00122-005-0185-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Rachis</tissue>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2010" name="BMC Genomics" volume="11" first="707" last="707">
            <title>Genome-wide SNPs and re-sequencing of growth habit and inflorescence genes in barley: implications for association mapping in germplasm arrays varying in size and structure.</title>
            <authorList>
                <person name="Cuesta-Marcos A."/>
                <person name="Szucs P."/>
                <person name="Close T.J."/>
                <person name="Filichkin T."/>
                <person name="Muehlbauer G.J."/>
                <person name="Smith K.P."/>
                <person name="Hayes P.M."/>
            </authorList>
            <dbReference type="PubMed" id="21159198"/>
            <dbReference type="DOI" id="10.1186/1471-2164-11-707"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="journal article" date="2010" name="Theor. Appl. Genet." volume="121" first="21" last="35">
            <title>CBF gene copy number variation at Frost Resistance-2 is associated with levels of freezing tolerance in temperate-climate cereals.</title>
            <authorList>
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                <person name="Dhillon T."/>
                <person name="Cheng H."/>
                <person name="Tondelli A."/>
                <person name="Pecchioni N."/>
                <person name="Stockinger E.J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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    <reference evidence="14" key="6">
        <citation type="journal article" date="2014" name="Funct. Integr. Genomics" volume="14" first="85" last="100">
            <title>The barley Frost resistance-H2 locus.</title>
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                <person name="Pasquariello M."/>
                <person name="Barabaschi D."/>
                <person name="Himmelbach A."/>
                <person name="Steuernagel B."/>
                <person name="Ariyadasa R."/>
                <person name="Stein N."/>
                <person name="Gandolfi F."/>
                <person name="Tenedini E."/>
                <person name="Bernardis I."/>
                <person name="Tagliafico E."/>
                <person name="Pecchioni N."/>
                <person name="Francia E."/>
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            <dbReference type="DOI" id="10.1007/s10142-014-0360-9"/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">subsp. vulgare</strain>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="807"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="825" mass="94250" checksum="27494F02B41EA545" modified="2017-11-22" version="1">MSGGFFRGTSADQDTRFSNKHAKLLKTQKFASELDHQVDMSKVKMDVMKPWIATRVTELLGFEDEVLINFIYGLLDAKEVDGKYIQIQLTGFMEKNTIKFMRELWSLLLSAQQNASGVPQQFLDAKEAEIQQRKAEEARIAQEIQKKREKDGRDAELDKRKMMAGDTGNSRSHGDSLGSALNNPNVDAEKEKELDLKHSSRTKSREHRRPRSTSLSPRGRQRSISPRRRSPSPSRQRSSRRSASPRRSVSPRKHSPRIAPSMSRRRSPYSRRSPSVPRSPSPRRRFPIRRRSPPSGRRRSPSPYRRRSPARPPRRSPSPARRRSPPRHRLPADRHRSPSPGRRRPRSPSPVRRRPRSPSPRRHRRSPGRRQSLSPHRSPQLRSPKRQRKSPISIRTFSANRPVSPQRRRSSSPHSRSPNRSHRSPSRDSDKGTNGAPSTKGRDLAQRNQERSSRESPDFEHRRLTKSLRSPTNDAERDSTHGSPLKDTGKHMPSQDSINTSGDEEEGGRARENARKANSSRRISKDFSADLQPKEVNVDNSIPAEKSSSKSRQDGGKDVPRKYDNQPSDSSEDALDGTRMKRQGDSPDDSRGKRQSPSRAGDSYPKDGINNERAKMDSHGASYDAIAAKKYSGKVDEASQSDGGSPLQKAKKRTYDSKHIDRHSSGSEESEKHRSQSEKRRHKKGHKHKKHYDDSSDSDSDSDDKESKRRRREEKKLRKEERRLRREERHRRRADRHASKQRLKYAGSPLSDLEKDRQSGSDADVGKKGSYTPREEPDPNKLEIELRQRALESLAKKSKHVVTPSSDVEKDHESDSDADVRKRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287PHF3</accession>
    <name>A0A287PHF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine synthase</fullName>
            <ecNumber evidence="3">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449306</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044982405.1">
        <property type="nucleotide sequence ID" value="XM_045126470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287PHF3"/>
    <dbReference type="SMR" id="A0A287PHF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396290"/>
    </dbReference>
    <dbReference type="GeneID" id="123449306"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449306"/>
    <dbReference type="OMA" id="GYKCLII"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287PHF3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000061">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000077">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000049">
        <property type="entry name" value="Cysteine synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000098">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="8">
        <location>
            <begin position="53"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="439" mass="47001" checksum="10E52A2A1C8D2769" modified="2017-11-22" version="1">MAAATAAGAAAAAASISLFAYYLLFGKSGSKFPWTRTTGADDRRTRRKGLVEAVGNTPLIRINSLSDATGCEILGKAEFLNPGGSVKDRVAVKIIEEALKSGNLVCGGVVTEGSAGSTAISLATVAPAYGCRCHVVIPDDAAVEKSQIIEALGATVERVRPVSITHRDHFVNIARRRALEANIASAQTEPNDIQTNGSAYHDTKALNSRQTNGSAHASSEVPDTGKCYPNSDSKGGFFADQFENMANYRAHYEWTGPEIWKQTKGTVHAFVAAAGTGGTIAGVSRYLKEMNRNVRCFLMDPPGSGLFNKVTRGVMYTKEEAEGKRLKNPFDTITEGIGINRVTANFMMAELDGAYRGTDREAVEMSRFLLRRDGLFVGSSSAMNCVGAVRVARDLGPGHTIVTILCDSGMRHLSKFFNDEYLANHGLTPTATGLEFLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287QYJ9</accession>
    <name>A0A287QYJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Jasmonate O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287QYJ9"/>
    <dbReference type="SMR" id="A0A287QYJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0474510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0474510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0474510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0393050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0393050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0393050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0474510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0474510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0474510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0474510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15285" checksum="E8405E1A4B359232" modified="2017-11-22" version="1">MVHMNQGQGETSYAHNSSIQSAQQNRMKPLIEADIIDLCGSRSTLFPEKMLIADLGCSSGPNALALVSTAVKAIINHWLQFQQPPPEVCVLLNDLPDNDFNTVVKSLVMLRQSNNKPVVVSCDWCRTGRTGVVLREALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WIV0</accession>
    <name>A0A287WIV0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123410236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044959079.1">
        <property type="nucleotide sequence ID" value="XM_045103144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287WIV0"/>
    <dbReference type="SMR" id="A0A287WIV0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683160"/>
    </dbReference>
    <dbReference type="GeneID" id="123410236"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0566720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0566720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0566720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0683160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410236"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287WIV0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46713">
        <property type="entry name" value="F13M7.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46713:SF5">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="348" mass="40093" checksum="A2D45A8EF92A0A52" modified="2017-11-22" version="1">MAVPQVDKKMLGELEVMGFPAVRSIRALHYSGNSNLQSAINWLLEHESDSDIDQLPLVSREISIECGDTLNEVRNSAQGMRTHVQERKSEEQATARNQKETSEVESEVNANEHEEEDRKRILALHKSKRDEEEKARGRIRNQLQEDKRERIQAAKDVMEAKRTLEENQRRRMMESRKAEQEEEKRARERIRQRIDDDKAERRRGLGLLRENTVASPPIVTLAKVKPIEPVVTSEQLRDCLRTLKKSHKDDNARVKRAFQILLKIVANIVKNPEEDKFRRIRLSNPVFKDRVGNLQGGVEFLELCGFQKLRNNSYLVMPRGKVDVALLNSAAVQVASAMENPYFGLLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W9K7</accession>
    <name>A0A8I6W9K7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="6">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123429245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044969235.1">
        <property type="nucleotide sequence ID" value="XM_045113300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W9K7"/>
    <dbReference type="SMR" id="A0A8I6W9K7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0103940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0103940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0103940"/>
    </dbReference>
    <dbReference type="GeneID" id="123429245"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0085380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0085380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0085380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0103940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0103940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0103940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429245"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000169">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000170">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0103940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="519" mass="58567" checksum="232249CFCA206AE7" modified="2022-05-25" version="1">MERKRVAIIGSGVNGLTACKHLLERGCRPAVFEADTLLGGVWARAPDCTSLQSERPMYQFSDFPWPESVTEVFPDRRQVADYLDAYARRFGVLDCVRFGRRVVGMEYVGVGEEEVAAWEEWAGCGEAFGSGEGEWRLTVADAEGNMETHVADFVVLCLGRFSGVPNIPTFPPGKGPEAFDGEVIHTMDYAKMGTKKANEMLKGKRVTIIGYLKSALDVAAECADVNGIEHPCTMVVRTKHWIIPSYYAWGFPISNLYLNRFSEFLVHKPGEGFLLCLLATILTPLRWLFSIFAESYYSIPMKKHDMVPDHSLFEALATCLIAITPKDHYKRLDEGSIVLKKSKTFSFCKEGVLVECESSPIKSDVVIFGTGFKGDQKIKNMFTSEYFQSIAVGPTSSTVPLYRECIHPKIPQLAVLGYSESLSNLYTAELRAKWLAHFIDGGFRLPSVKAMQSDILEWEKFMKRYSRVYFRRSCIGLLHIWYNDQLCQDMGCNPRRKNGIFAELFDVYGPHDYVNLHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WAQ4</accession>
    <name>A0A8I6WAQ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WAQ4"/>
    <dbReference type="SMR" id="A0A8I6WAQ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005430.1">
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    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="46"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="24957" checksum="3BD98DA70EA282A7" modified="2022-05-25" version="1">MPTRFPKVLAHGTTMLDVVYTNESREVPFFLEQLKEKWLDAAMDHEKFLGLDLEYTADQRGVAVIQLCFARHVLIFQWASGDKHCPKLMDFLRSGITFATVDIRNDKLKMRYSFGIEIPTGYLIDLQTVFRLRHVRTSMAHMAVALIDEEYGDMKTGFPKSQHKLWEKAPLDPISIEYAAKDAYVSHELYRKIRVVNYGQRHLEDGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WD49</accession>
    <name>A0A8I6WD49_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WD49"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0009050.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0006720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0006720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0009050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0009050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0009050"/>
    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="53"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
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        <location>
            <begin position="106"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
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        </location>
    </feature>
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        <source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0009050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="250" mass="28479" checksum="6829652F3F160D64" modified="2022-05-25" version="1">MAARKGPWTEKEDAQLVWFVRLLGERRWDFLAQVSGLRRTGKSCRLRWVNYLHPGLKRGRITADEERLILTLHAEWGSRWSRIARKLPGRTDNEIKNYWRTHMRKKAQEEKMMAKNKASSDSTAANSLTTTTTCSASTPTATSSTTDAPQTSMGDEAEEEASTSATEEKKAEVVQYCYAVDMDQLWNDIAASERYPDMMMSWGAAGHAAVAAPALEPSSPVWDLCEQDYYSLWRIDDQEYYNKIDQHALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Ubiquitinyl hydrolase 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC123424561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045108191.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106720"/>
    </dbReference>
    <dbReference type="GeneID" id="123424561"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.2HG0087510"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase C65 Otubain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042467">
        <property type="entry name" value="Peptidase_C65_otubain_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF18">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53244" checksum="07907C1224AB32D0" modified="2022-05-25" version="1">MKFKIKLKNPLRGASAASSRGGGGGDGDGATPPTSPRLPVAPPPASPSLDAWAPPTSAPPLQTPGPSPRPAGKDTDEGPDDEDWQEVEDDEVELEEGEIEDDADPGEEEEDDQDSQGGSLPELPLGTEDYPQTLSQLLVGETYPDAYLKMEFKPPINDRVVTGGLRCPDAYLGRPLLHGETYLKPPKDQFRCSDALPTVLGHVEQLSELVRESPDNFIIQEKTKILSKHYVRFRRTLQDGSCFYRAFMFSYMEILRHMQDKQDEVTRLIECLDLSKDRFSRLRWHKADFSIDPEKYFSRVVSELEEVLNVIAAGCTSEWLYQRSLQESFSGRVIPLLRLLTETEIRTEKFYRQYIPENLSVREFCRKAVQSLDAEASTIQMRALTFALGIPLRVEVVDNSLSDQGVLVKRLDFFRGTDWDKGPLRLTRGYLSSRTAPIQLKQGSYDADLLSPDGTPMLTLLCRRGHCDILYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLL2</accession>
    <name>A0A8I6WLL2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123416014</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in cooperative interactions with calmodulins or calmodulin-like proteins. Recruits calmodulin proteins to microtubules, thus being a potential scaffold in cellular signaling and trafficking. May associate with nucleic acids and regulate gene expression at the transcriptional or post-transcriptional level.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962706.1">
        <property type="nucleotide sequence ID" value="XM_045106771.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962707.1">
        <property type="nucleotide sequence ID" value="XM_045106772.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962709.1">
        <property type="nucleotide sequence ID" value="XM_045106774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLL2"/>
    <dbReference type="SMR" id="A0A8I6WLL2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076760"/>
    </dbReference>
    <dbReference type="GeneID" id="123416014"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0062220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0062220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0062220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123416014"/>
    <dbReference type="OMA" id="FAWESST"/>
    <dbReference type="OrthoDB" id="1704267at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF10">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4005" evidence="6">
        <location>
            <begin position="227"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="342" mass="37115" checksum="762559D044933756" modified="2022-05-25" version="1">MGRAMRWLKKVLTGGKKEGSKDQSTAAPPIERRRWSFAKARSSVADASRRPSVTAVVAGELSQSRPCGCGEAGAAVLIQKAFRGYLARKALRALKSLVKLQALVRGYLVRKQAATTLHRLQALMRLQASSHALKNLSSRRSIEQERKASLPVAHRRRLSEGGAGDAGFDRSPRIVEMDTCQLRCRSSRIAGRYAADPQAPPPSSPLAYLCKPPSRLQLRELEPRQPKTTQNTPRLPAVVPSGSPAKGRPSCGGGGGRESSSPRYMADTASSVARGRCQSAPRQRHGHNGAAAPGLPRAGSRKAAPQSQDSFSFKSSEATSRVEDYSEMSDEVTRDYYLDQLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMG6</accession>
    <name>A0A8I6WMG6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123431475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971215.1">
        <property type="nucleotide sequence ID" value="XM_045115280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMG6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096460"/>
    </dbReference>
    <dbReference type="GeneID" id="123431475"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0079700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0079700.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0079700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0096460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431475"/>
    <dbReference type="OrthoDB" id="649712at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF109">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096460.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="58852" checksum="6700A60396044B3B" modified="2022-05-25" version="1">MDILISAIAGDLVGRSMSFLISKYFQQQPDVSKIAQRLQSVVLRIDTVVEEAQGRDITNQGMLCQLKMLREEMYRGHYVLDALRFRPALDEEGEEGMSHSLAVPRKSSPAKRLRFSRTSSSSREALLIDTNRNIRQELQQMVDTLEDTMDGMKEFLFFLELYPRMLREPYGTYLFLDNCIFGRQTERRRILNFLMCPSATPDLAILPIVGPIRVGKSTLVENICRDDSVRDRFSMILFFPEGSLKDERVVNLRENNIKFRHQNFASQNRLLIIIETANDINEETWRRLKSSATCMTPCGESKIIITSWSDRIVNLGTTEALRLDYLPQEAYWHFFKSLVFRSTNSDEQPKLATMAMEIALELRQCFTSARIAAGILRDNFNARFWRTVLDCVRESKQTNLLMFDQHPYLRLREDAPMYCWRLVKGHRYFFICNHHQSESSENVPKINLQDIMLGCGGKLPCGEFEALAWRSRIPPYYNYTVSCKMQAPQLTVGRKKRVHQEEEHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNY2</accession>
    <name>A0A8I6WNY2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044964987.1">
        <property type="nucleotide sequence ID" value="XM_045109052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNY2"/>
    <dbReference type="SMR" id="A0A8I6WNY2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114600.1"/>
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    </dbReference>
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            <end position="158"/>
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        <location>
            <begin position="1"/>
            <end position="105"/>
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            <begin position="8"/>
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        <location>
            <begin position="83"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="458" mass="51137" checksum="DDFAC232E22F7C0C" modified="2022-05-25" version="1">MMPSSSSSRRRRRRRGNRNRLPPPAPITEAGEVTSDEAELPSAVAYRLERIPFSRPAPPKPKHAEPQPQPHGATHPSSSFSSSRRRRGRRRRKGSAPAPAPAPAAEEARDWAGLLLDALCAILHKLDHVEILTGAGQVCRSWRHAARDVPALWRRIDMRGHADIHELDLRGMAQVAVRRSAGQCEAFWGEYAAHEGLILFLGDQAPSLKSLRLISCYRVFIEELVEALNKFPLLEELELSLCSNIGGSHMFDVAKACPQLRVFRMSELRFHNLQDDSDGEDADSEFRYNKNDDAKGIATMLQLRSLQLFANNFTNEGLTAILDNCVHLESLDIRHCFNVVMDDALRAKCSRIKTLRLPNDPTDDYDIEALSPIWSGIGMGVDTDSDGDCVYGGPDYILDSDEYDDYCDPERYLDGVYIGRRVTLMDLLDSGYSMLGVSLNLCSIRVTECCAKVVNVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WPN4</accession>
    <name>A0A8I6WPN4_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0003240.1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0003240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="20958" checksum="E99E291B5486C9B7" modified="2022-05-25" version="1">MWHYTGPEDTTRTNVVGVTKERVTSWVLQITGPYENPKGARRVKPYSADHPPPNQAWTNWFSPVSNGNPEEEEEEGGQEDSVESTEYVSDSGESEEESSEEEEEDEDQDSPPPRPEHRTKRRHDPAVPSAPPTSPSAPPTGPVVPSVRSTKRARDAAAEPAGQSFKAPKPSGPKPRKALPRMRVAIPVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQZ6</accession>
    <name>A0A8I6WQZ6_HORVV</name>
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            <fullName evidence="13">Protein RRP5 homolog</fullName>
        </recommendedName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">rRNA biogenesis protein RRP5</fullName>
        </alternativeName>
    </protein>
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        <name evidence="19" type="primary">LOC123410099</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential for the generation of mature 18S rRNA, specifically necessary for cleavages at sites A0, 1 and 2 of the 47S precursor. Directly interacts with U3 snoRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with NF-kappa-B p50/NFKB1 and NF-kappa-B p65/RELA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044959020.1">
        <property type="nucleotide sequence ID" value="XM_045103085.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WQZ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0014620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0014620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0014620"/>
    </dbReference>
    <dbReference type="GeneID" id="123410099"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0014620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0014620"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05703">
        <property type="entry name" value="S1_Rrp5_repeat_hs12_sc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04461">
        <property type="entry name" value="S1_Rrp5_repeat_hs8_sc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000148">
        <property type="entry name" value="protein RRP5 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000212">
        <property type="entry name" value="Ribosomal protein S1-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000263">
        <property type="entry name" value="Ribosomal protein S1-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000314">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000159">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000179">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057300">
        <property type="entry name" value="OB_Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24682">
        <property type="entry name" value="OB_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="147"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="247"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="333"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="419"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="509"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="597"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="681"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="771"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="883"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="1066"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="1164"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="1261"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="1358"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="1448"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1523"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1579"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1525"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1604"/>
            <end position="1614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0014620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1901" mass="210861" checksum="B1D0E544F2EE2159" modified="2022-05-25" version="1">MAPHGDRKKGKGADRPGKPGLGPNRKEFKNHRKEEGEKAGGVDEEHEQEQQTAPHPAALFNAADDGDFPRGGRSLLSRDEMAEARTEAEAEFEKEERMGKKKRKASASSSGIDGDDDLGTLFGGATATTGKLPRFANRITLKNISPSMKLWGVVIEVNQKDIIVSLPGGMRGFVRTEEVSDIALHGNSKDNEGSVCAEVVHVGQLVPCMVLQVDDDKKEGKVHKRVWLSLRLSRMYKGLSLDAIQDGMVLTAQVKSVEDHGYILYFGVSTFSGFMPKAGKETVKIESGQLIQCVVKGIDKTRSIIHLSSDEDLISKSIIKDLKGLSIDHLIPGMMMSARVHSVLENGVMLSFLTYFTGTADIFNLLSSFPSGNWKDDYSKNKKVNARILFVDPSTRAVGLTLNQHLLRLNVPPINVKVGEIYDKSRVLRVDKKAGLFLEIPSPTPSPGFVSIHDVSDKDVKNVEKKFREGSLTRVRVLGVRHLEGVALGTLKESAFEGSVFTHADVKPGMVVRAKVVTVEPFGAIVQFSGGVKALCPLPHMSELDHVVKPPKKFKAGAELLFRVLGCKSKRITVTYKKSLVKSKLEVLASYADAKIGLVTHGWITKIEKHGCFVRFYNGVQGFVSRSELGLEPGTEAESVYHVGQVVKCRIVSVVPTSRKLYVSFTMSSHRVSQADTAKVGTIVSGVVERLTPATVVVSVNGFSKGSILNEQLADHHGQAAQLKNLLKPGHEFNQLLVLDTEGQNLVLSAKHSLINTANDIPSEISQMQAGAVVHGYICNIIEAGCFVRFLGHLTGFSPKDKAVDRPIEKLSDVFCVGQSVRSHVLNVNAESARVKLSLQQSMCSSPDCSFIQGYFLLDQKIAALKYPGPSTSHDWVKYFGIGSLVKGEVGAVEEYGVILNFKDYPDVVGLIEHHQLGGSSVKVGSSVKGLVVDLSDGVVNLSLKPELVGSVSKDGKKKKRHRAAVLDLELREEVNAVVEIVKDSYVVLSVPEYNHAIGFAPLMDYNSQLLPQHHYDNGQCINVVVGSIPSSDPSGRLILLPKGSAQDSSISGSKRAKKKSDYKVGSLVEAEIIDIKPLEVILKFGVNLHGRIHITEVFEEECSEHPFSKLKIGQKVHARIVARAEHSAKSGRNLKWELSIRPSLLKDLEKLTPSEAELNHSINDIVRAYVVKVDGEWVWLTVSRKVMAHLFILDSSVEPSELKEFQQRYSVGQAVKGRIIGVNREKRLLRLKALDNQCMPENIDDTQKTVSSIAEHTKEGDIVGGRIQRVLPGVGGLVIQIGPHLHGRVHYTEIVDSWVAEPLSGIHEGQFVKCKVLAVSRQSEGSVRVDLSLCSRNLDDLTTCTPRFEKVNDLCPGTEVKGYVKNVNAKGCFIMLSRMVDARIILSNLSDEYVQNPQKDFSVGMLVQGRVLSVESLSGKVEVSLRKSTGSKSQKLDAISYSDLHVGDIVDGQVKRVESYGLFVTIKSSELVGLCHISELSDEPVLDINACYRAGDMVKAKILKIDQDRHRVSLGLKESYFDSDMTDDENDNENDGERVPMDISRAPQISGGFNSTSFLPGPEPRASIPALQVTLDEYEGSDQEGDQKGHEIANGSESNVKKSDRRLKEKARKQREIEISALEERALQKDTPQTPDEFEKLVRSSPNSSFVWIKYMAFLLDLADVEKARSVAERALRTINIREEEEKLNVWVAYFNLENEYGSPREDAVKKIFQRALQYCDPKKVHLALLGMYERTKQNELADELFDRMTKRFKTSCKVWLRRIQFSLTQGKDVEYIKSVVNRALLSLPQSKRIKFLTQTAILEFKCGVAEEGRSRFELILREYPKRTDIWSVYLDQEIRLGDTEIIRALFDRVTCLSLPPKKMKFLFKKYLRYEKAQGDEERIEHVKQKAMEYVEISRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR18</accession>
    <name>A0A8I6WR18_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable glutamyl endopeptidase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Serine-type protease active in vitro against the LHCII N-terminal. Cleaves its substrate on the carboxy-side of Glu residues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase S9D family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR18"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0109980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0109980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0109980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0090260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0090260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0090260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0109980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0109980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0109980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000049">
        <property type="entry name" value="probable glutamyl endopeptidase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF21">
        <property type="entry name" value="PEPTIDASE S9 PROLYL OLIGOPEPTIDASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="12">
        <location>
            <begin position="721"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0109980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="889" mass="99874" checksum="3CF2FD2F8CAE9A4E" modified="2022-05-25" version="1">MLPRRLGQRLCFACANPAAAATPSSAPPRRRLRLLPHGGHASPIGFSATAMCAAGSLPAGPVPQDDGAVSGYRLPPKEIQEIVDVPPNPSYHLSPRRDRIMFLKRRAMPPLSELAKPDKVLAGIRIDPGSNMRSRMSFYTGVSIHLLMDDGSLGPEKVVHGYTDGSKINFVTWSPDGQNVAFTVRYEDEAGSDSNLVLWVANAESGEARPLFKQTDIRLNAIFELFVWVDHSTLLVCTIPSSRGDPPKKPLVPFGPRIRSNEQKNVIQMRATKEMLKDLHDEELFDYYATSQLVLVSLVGIVKPVASPAIYTSLNPSPDEKYLMLASVHKPYSSIVAYKRFPKKVELWTVHGRFVREVCDLPLAENIPIAANSVRRGKRLIRWRPDMPSTLYWVEAQDGGDANVEVSPRDIVYMEPAEPLNGEKPHVLLKLDLRYRRISWCYGLHALVYEYWQKTRRTRTWVISPDFNDFSPRLLFDRSSEDAYSSPGSPMMCRTPTGTLVIAKVKTGYEGTYILMKGQGATPKGSVPFLDLLNITTGAKERIWESGEDKYYESVLALMSYHPKCEIQLNELKFLISKESRREATQYYVSIWPDKKKVQITSYPHPYPQLASLQREIIRYERDDGVKLTATLYLPPGYNPSKDGPLACLIWSYPGDFKSREAAGQVRRSPNKFARINNSFPLLWLARGFAILADPTIPIIGEGDQEANDRYIEQLIASAEAAVNEIIRRGVAHPDKIAVGGHSYGAFMTANLLAHAPHLFCCGIARSGAYNRTLTPFGFQKEVRTLWEATDTYIKMSPFMLANKIKKPILLIHGEEDSKVTTAMQSSQFYNALKGHGVPCRLVILPFEGHRYAARESVMHVIWETDRWLQKYCASNSRSTQIIESSGEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR52</accession>
    <name>A0A8I6WR52_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123443444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">subsp. vulgare</strain>
        </source>
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    </evidence>
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            <begin position="34"/>
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        <source>
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    <sequence length="114" mass="13599" checksum="89AF6E88F5F93490" modified="2022-05-25" version="1">MVLISIPVGPHLVHDSNVEVCEPEIPTNPLVERLYLVEKENEYLKEKLKRIEEEKMKLELYVADVVNDHKIKMEKMRLKIRKIRKYAIDSEAWYNYDWINCYPSCDLDHICCCS</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">cv. Morex</strain>
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    <reference evidence="10" key="2">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">cv. Morex</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.110.10:FF:000001">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035268068" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0151370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="11614" checksum="4B9CFA5403D0C8FA" modified="2022-05-25" version="1">MEIVAIALILATMLASKAVAQNNGCSSVMMTLSPCLDFIGSKALAPGFSCCTTLAGVVQTDPRCLCMVLDGTAASFGIAINHTRALELPGFCKVQAPPISQCTGLLILRSVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2R0</accession>
    <name>A0A8I6X2R0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2R0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0216640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0216640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0216640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0180140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0180140.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0180140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0216640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0216640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0216640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0216640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="16038" checksum="09760175AAFF542D" modified="2022-05-25" version="1">MNFPDIRTLQQALDLAPPPRLNSAQDRAEHTAMQRRLLVAQEDERVMAEWRRRHPEDVAYEQEYWERRREEDTRRRREERLDRRRRKALACAQADLVNAGGSSFFTEEDERWFDIWLSTSDDTDDDGGADDWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2X3</accession>
    <name>A0A8I6X2X3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2X3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0192780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0192780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0192780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900055">
        <property type="term" value="P:regulation of leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd07325">
        <property type="entry name" value="M48_Ste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000007">
        <property type="entry name" value="Peptidase M48 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="8">
        <location>
            <begin position="128"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="336" mass="37029" checksum="548A9BB7B0AE5016" modified="2022-05-25" version="1">MAACLSPTLPRHTVHRRRGWAPKPPSSSSSPSTAAYSRRHRQHQHQIVATRSGAISARAASPAAAAAAARGLDADDIRHPLDKQNTLLLKAIPGLNDIGKALLGPVSEQVMVLQNIGSSVLVSENQLPELHQLMVEAAKILNTEAPDLYIRQNPVPNAYTLAINGKRPFVVVHTSLVELLTRKELQAVLAHELGHLKCDHGVWLTFANILTMGAYTVPGFGMVAGFLEEQLFRWLRAAELTCDRAALLVVKDPKVVISVLMKLAGGCPSLSDQLNVDAFLEQARSYDKASSNPVGWYIRNAQTRELSHPLPVMRAREMDEWSRSQEYRTLLHKMFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7L4</accession>
    <name>A0A8I6X7L4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphatidylserine decarboxylase proenzyme 2</fullName>
            <ecNumber evidence="16">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="16">Phosphatidylserine decarboxylase 2 beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="16">Phosphatidylserine decarboxylase 2 alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="16" type="primary">PSD2</name>
        <name evidence="20" type="synonym">LOC123445581</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. Contributes only to a minor proportion of PtdEtn production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="16">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="16">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The C2 domains have an essential, but non-catalytic function. They may facilitate interactions with other proteins and are required for lipid transport function.</text>
    </comment>
    <comment type="PTM">
        <text evidence="16">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type II sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7L4"/>
    <dbReference type="SMR" id="A0A8I6X7L4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0323600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0323600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0323600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0270080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0270080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0270080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0323600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0323600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0323600"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000200">
        <property type="entry name" value="Phosphatidylserine decarboxylase proenzyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000194">
        <property type="entry name" value="Phosphatidylserine decarboxylase proenzyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00663">
        <property type="entry name" value="PS_decarb_PSD_B_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033179">
        <property type="entry name" value="PSD_type2_pro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF17">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 16" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 16" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5035349756" description="Phosphatidylserine decarboxylase 2 beta chain" evidence="16">
        <location>
            <begin position="1"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035349755" description="Phosphatidylserine decarboxylase 2 alpha chain" evidence="16">
        <location>
            <begin position="606"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="18">
        <location>
            <begin position="20"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="16">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="16">
        <location>
            <position position="518"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="16">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity" evidence="16">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="16">
        <location>
            <begin position="605"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="16">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0323600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="648" mass="71716" checksum="C7489C2320024385" modified="2022-05-25" version="1">MGHAHSTSRHNGRAVGGAANGAEPQLPSRFSRFRSRLRLRRHRGRDRCGGAAAGDAGDSGKGVAADEFAGIARIRIVKADLRFKDKFFACLSLGERTYRTETSDSTHRPVWNSEKKVVIETNGPHIARISVFETNRFSKNTLVGYCEVDLFDLLTKDLEEEHVEDLALLDPSSSITTMGTISVSCYIEDPVETEQSFARRVLAIVDYNEDGKLSLSEFSDLMKAFGNGLAVNKIEELFRRADTNGDGIVDMDELAALLADQQEKEPIISNCPVCGESLGKYDKINDMIHMTLCFDEGTGNQIMTGGFLTDKQAAYGWMFKLSEWANFSSYDVGLRSGSTASHILVFDRRTKRLVEEAIDGKIVLSMRALYQSKVGLTLIDSGVKDILKNLSEKQGKKMNSPESAKDIPKFLELFAGQINMDETKDPIESFKTFNEFFIRQLKPSARPIAHTDDDSIATCAADCRLMAYSSVDESTRFWIKGRKFSIEGLLGADAHYNAFKDGSLVIFRLAPQDYHRFHVPVSGTVEKFVEIPGCLYTVNPIAVNSKYCNVFTENKRVISIISTSEFGKVAFVAIGATMVGSITFVKKEGDYVHKGDEFGYFSFGGSTVICVFEKDAIQFDADLLANSERSLETLVEVGTTLGVSKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7Z7</accession>
    <name>A0A8I6X7Z7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCB4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123428131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968231.1">
        <property type="nucleotide sequence ID" value="XM_045112296.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968232.1">
        <property type="nucleotide sequence ID" value="XM_045112297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7Z7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204320.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204320.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204320"/>
    </dbReference>
    <dbReference type="GeneID" id="123428131"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0169910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0169910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0169910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0204320.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204320.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428131"/>
    <dbReference type="OrthoDB" id="439612at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010190">
        <property type="term" value="P:cytochrome b6f complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021325">
        <property type="entry name" value="CCB2/CCB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044705">
        <property type="entry name" value="CCB4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34943">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34943:SF2">
        <property type="entry name" value="PROTEIN COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11152">
        <property type="entry name" value="CCB2_CCB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204320.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="31215" checksum="86E9ABB619D0735A" modified="2022-05-25" version="1">MEMTLLRSRTIAPLPSTPRPASAQRRRCRLPSRTVFPPQPPPPGRGDRVCSRAMRQRQEWVGDWVRSNDTLVRALPILVGGASLLAVLLNRAVSGIAAVSDASSSQSRADILTLALSVTDILAGLVWLSIRPKTISPVVPRGVDCKRVDADVSSSALRELLWTWDSLAAATCCKSLVVVYGGNCKLQIGIAAASPEDGNAVNVDAQKFMQGSLYKSAMESKKQSYLANLALYPGRIELPFLPANTQALILQPIGDKGIAVIGGDTIRGFTNLDQAWIAMIADKLDATLSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8A8</accession>
    <name>A0A8I6X8A8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import receptor subunit TOM7-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123443921</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976421.1">
        <property type="nucleotide sequence ID" value="XM_045120486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8A8"/>
    <dbReference type="SMR" id="A0A8I6X8A8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273630"/>
    </dbReference>
    <dbReference type="GeneID" id="123443921"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0226730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0226730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0226730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0273630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443921"/>
    <dbReference type="OrthoDB" id="284357at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012621">
        <property type="entry name" value="Tom7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34944">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34944:SF11">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08038">
        <property type="entry name" value="Tom7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="8754" checksum="A32E8ED5E7C14B0B" modified="2022-05-25" version="1">MASRPSLKPKPKGKGGKRGSSAAEDEQSTAAVAVRLAKEWSTWTMKTAKVVAHWGFIPLIIVVGMTKGDEPKPSLLQLLSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8X3</accession>
    <name>A0A8I6X8X3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123447135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979646.1">
        <property type="nucleotide sequence ID" value="XM_045123711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8X3"/>
    <dbReference type="SMR" id="A0A8I6X8X3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334460"/>
    </dbReference>
    <dbReference type="GeneID" id="123447135"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447135"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000367">
        <property type="entry name" value="glycine-rich RNA-binding protein RZ1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000033">
        <property type="entry name" value="Glycine-rich RNA-binding protein RZ1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48031:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48031">
        <property type="entry name" value="SRA STEM-LOOP-INTERACTING RNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="318" mass="34677" checksum="E57C04BC01AFC0C3" modified="2022-05-25" version="1">MAGKEDSRIFVGGLSFHTDERKLEDAFRRFGKVVETQIMLERHTNRHRGFGFVTFSDPRAVETAINDMHNKELDGRTISVNRAEPKLQTDDTRYSGGGGGGDRGDYRGGKGDGPPPGNCFECGRAGHWARDCPNPGGGRSGRFPSKFSGGGGRGDRFSGSDRFGDRYVDDRYDSGRYGGYREPIDSRDRYDGGRDRYASDRYPSGGDRYGTDRYGAAPDRYAPSGGGGGYGRERERSYERDTVRGGAYDRSGPRGGASYDRDGPRGGIGGGYDRDGPRGGGADYGSGGPTRYEGGGYRERPAPYDRPSRGGGRFDDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAW3</accession>
    <name>A0A8I6XAW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DEK-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123445278</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044978178.1">
        <property type="nucleotide sequence ID" value="XM_045122243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAW3"/>
    <dbReference type="SMR" id="A0A8I6XAW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311510"/>
    </dbReference>
    <dbReference type="GeneID" id="123445278"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0260150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0260150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0260150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0311510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445278"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000779">
        <property type="term" value="P:regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000220">
        <property type="entry name" value="DEK domain-containing chromatin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044198">
        <property type="entry name" value="DEK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468">
        <property type="entry name" value="DEK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468:SF1">
        <property type="entry name" value="PROTEIN DEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DEK-C" evidence="8">
        <location>
            <begin position="396"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="449"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="488" mass="54147" checksum="037A57B2BD555A6D" modified="2022-05-25" version="1">MATAAKPADPAPADPPADDPMDTEEHEEEPASAEEHEEEEQPQKKRAKGKRKRAPATPATGRPSRERKTVERYSELTPRSTPAKKSASILQGSGTKLKDIPNVFFKLTKRKADDNLQSLHSLLYGRKSTAHFLKRNLSQFSGYVWTDNPEKQRSKIKDKLDKMNKEKLLDFCDILDVQAKSTAKKEEVSAKLLEFLESPCITRAVVISAVKKGKKRRMKSKGTSKATAEGGEKKKRKSQKQAVEAEKEDDDEDGAGPADSEDLSMGGSDEDAEVKEDAKSDEEPEVTPVKKKSKDDKQGNKEAGSKAKEKDASVKKTPTKSVKSVSKPDVELENKKAVKRTPKNSTKQSKTPVDKAKKKVAKPKKDDGKESQNNSKARKKPGPKATGENKGKGKVAPTTKQLHAVVSNILKEVDFNTATLADILKKLGDHFDMDLMDRKSEVKRIIEEVINSMSDDEGEEENEEEDAEENGRKEEDSKEDPDEEKDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAZ2</accession>
    <name>A0A8I6XAZ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein 234 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123431478</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044971216.1">
        <property type="nucleotide sequence ID" value="XM_045115281.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971217.1">
        <property type="nucleotide sequence ID" value="XM_045115282.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971218.1">
        <property type="nucleotide sequence ID" value="XM_045115283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAZ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096480"/>
    </dbReference>
    <dbReference type="GeneID" id="123431478"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0096480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431478"/>
    <dbReference type="OrthoDB" id="43458at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018908">
        <property type="entry name" value="TMEM234"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10639">
        <property type="entry name" value="TMEM234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="12960" checksum="863A0975E9651C99" modified="2022-05-25" version="1">MAAVGGDAASMVAVGLVWGATNALMRRGALVWDRRSRSLPAGTGAVRRWADLLLTWQYSAPFLANLSASAAFFRLLGDAPISVAVPVTNATTFAATAVAAALLGEATRAAPAALGTALIVLGVWVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBV5</accession>
    <name>A0A8I6XBV5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123429276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044969267.1">
        <property type="nucleotide sequence ID" value="XM_045113332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBV5"/>
    <dbReference type="SMR" id="A0A8I6XBV5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0105290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0105290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0105290"/>
    </dbReference>
    <dbReference type="GeneID" id="123429276"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0086520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0086520.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0105290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0105290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0105290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429276"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47122:SF9">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47122">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="106"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0105290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="200" mass="23023" checksum="B50EE91732F4FEA1" modified="2022-05-25" version="1">MDTEFCSVYDVPIDQLINVPPDESDLHRCWEDFGGQLVVPHCPSTYDEAPDATPLEPERGWRRKRGPRKRRVLLCTDQIWHLGNHFGDTSCPLPNTPTASEGQECARRTNNGHWTPEEVATLVDGVEDCGVARWSAMKKKWFPESVRSAINLKDKWRNLLESYTGNAERRRYLHLDKKLIKRIQKAALDNPYPKPRGTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDJ9</accession>
    <name>A0A8I6XDJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDJ9"/>
    <dbReference type="SMR" id="A0A8I6XDJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0259360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0259360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0259360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0259360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0259360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0259360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="92"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0259360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="470" mass="51176" checksum="558FDF4DD77B53AA" modified="2022-05-25" version="1">MASIPSDEVSKNGHGNGNGPSPPRAKRPRAAFISAAQIRDEFAHHDPAVARVNNGSFGCCPASVLEAQARWQRLFLAQPDAFYFDGLQPGLRRSRAAVAALVNAGDVSEISLVDNATTAAAIVLQHAAWSFAEGHFARGDAVLMLHYAYGAVKKSIQAYVARAGATVVEVPLPFPVTSPHAIIAEFHAALAVAKAGGRKVRLAVIDHITSMPSVLIPVKELVAICRQEGVDKVFVDAAHSVGQVPVDVRDIGADFYTSNLHKWFFCPPAVAFLHTRKGGPIASQLHHPVVSHEYGNGLPMESGWIGTRDYSAQIVVPEAIDFVNRFEGGIEGIRSRNHEKVVEMGRMLAEAWGTFLGSPPVMCGSMVMVGMPSCLCIESDDDALRVRTMLRKDFKVEVPIYYNTRQVEVQEIAKDNNSDPVTGYVRISHQVYNVKEEYERLRDAVNKLVAEGFTSAELRPAEKIIYSTEV</sequence>
</entry>
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    <accession>A0A8I6XIQ9</accession>
    <name>A0A8I6XIQ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314460"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262720.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262760.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0314460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0314500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF192">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="204"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="315" mass="34188" checksum="89AD4FBBB08A78B1" modified="2022-05-25" version="1">MAMAAVLPAAATETHVRFCGKYTITAMEKPSNQPCTVRLRSDLILSHGVRGVDGRYVRVTTDPVARALDHRVELADAAVLRSDEACSDLIHDMLAEAPVTAGYDLAADNWMDSSTDSDRTPLDIAIAIVTYVREEVDGIVDMGCVAPSLDFNVHMPELRVTVVYSEPKALLLACKEAAAAAANAEAAGAPAPGRKRCRESGEPCAICMGDEDGSWDEEETVTLPCSHAFHSGCILTWFHRAPTCPTCRCDIMECFSFVRSSPPPEQLTDDVAEDEFELVIGFLAHGEQLVRPAGTDDEPQFIDSDDPEEEEFIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJ08</accession>
    <name>A0A8I6XJ08_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Triticodae</taxon>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
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        <location>
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            <end position="151"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPM2"/>
    <dbReference type="SMR" id="A0A8I6XPM2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294310.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294310.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0294310.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294310.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000455">
        <property type="entry name" value="Autophagy-related protein 18f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045142">
        <property type="entry name" value="BCAS3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022175">
        <property type="entry name" value="BCAS3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048382">
        <property type="entry name" value="BCAS3_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268:SF7">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 18F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268">
        <property type="entry name" value="BREAST CARCINOMA AMPLIFIED SEQUENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12490">
        <property type="entry name" value="BCAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21034">
        <property type="entry name" value="BCAS3_WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCAS3 WD40" evidence="3">
        <location>
            <begin position="76"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="BCAS3 WD40" evidence="3">
        <location>
            <begin position="355"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="BCAS3" evidence="2">
        <location>
            <begin position="618"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294310.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="880" mass="94485" checksum="19B301E068016CEE" modified="2022-05-25" version="1">MRNGAQAPRGGTGGGGGGGLFSARSLSSYMRIVSSGASTAASTLRSAGASLVNSIASHDDDAGRDQVPVQWAGFDKLECGGDVLRQVLLLAYRSGFQVWDVEHADDVRQLESRHDGAVSFIQLLKKPIASKRGEDRFVDARPLLALAGGGTSTGNANSHDVNGPVFNGTNGTYHNSGSEKLPTIVRFYSLKEHGYVHSMKFRSAVYSIRCSPRVVAVSQATQIHCFDAATLELDYTLLTSPIVSPISGYGPLGLGPRWIAYSGSPVPVPNTGRVTPQLLSLSPIVPPPGSNGSVVAYYAKESSKQLAAGIVTLGDVGYKKLSRYYSDLIPNGNGNIKQGNAGFKANGVTNGHTIDSEYAGTVIVRDIVSKAMVVQFRAHTSPISALCFDPSGTLLVTASIHGRNINVFRIMPSVDGSASEDGPNGTYVHLFKLQRGITNAVIKDISFSDDSEWILISSSRGTSHFFAISPYSGSTSFRYSDNNLAENNYVVDSSVKHATHWSQNASPSLSLNQKMLSLSGPPVALSVVSRIRNGSNLLKGAVHGAAAFATGVSSPISGAIASTFHNCKGGSNSDGSFLCMKYYLLVFSPSGSIIQYVLHHSAEPDSGIDYPSDAISYGSQRETDTRFVIEPLQKWDVCQKKNRRDTAESNLYNDFDSGENNKIFQKVVRKGTSIYPSNVAATERLKLSTDEKHNYYISESELQTHVAQIPVWSRSGVHFQVIGSGTLEAYATDNISGEIEIEKVQTHNVESRSKNLIPVFESLHTSRFQQTRVNTPDSNRSGFLQRHKSGFSADGRLSSRSSCSSLDCMSEVPNSSDDNSVGQYLVEDSAAAAAVNKNLNVNHQAELVNNTESLNTEAQLGVVNSKDDCEDSELLPDLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQS3</accession>
    <name>A0A8I6XQS3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein EARLY HEADING DATE 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein RICE INDETERMINATE 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123442889</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transcription activator that acts as a flowering master switch in both long and short days, independently of the circadian clock. Promotes flowering upstream of HD1 by up-regulating FTL1, FTL4, FTL5, FTL6, EHD1, HD3A and RFT1. Seems to repress FTL11 expression. May recognize the consensus motif 5'-TTTGTCGTAAT-3' in target gene promoters.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975001.1">
        <property type="nucleotide sequence ID" value="XM_045119066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQS3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236170"/>
    </dbReference>
    <dbReference type="GeneID" id="123442889"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442889"/>
    <dbReference type="OrthoDB" id="6354171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF236">
        <property type="entry name" value="PROTEIN INDETERMINATE-DOMAIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="14"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="498" mass="53122" checksum="7570A068E020B2D0" modified="2022-05-25" version="1">MMLKDFAAIQQQQQQQQLALAAAAADENMSNLTSASGDQTSVSSHPLPPPSKKKRSLPGNPDPDAEVIALSPRALMATNRYVCEICGKGFQRDQNLQLHRRGHNLPWKLKQRNPNEVVRKKVYVCPEPGCVHHDRSRALGDLTGIKKHFSRKHGEKKWKCDKCAKRYAVQSDWKAHSKVCGTREYRCDCGTLFSRRDSFITHRAFCDALAEESARAVAVAVEEQQHPGMLYSHGGGSAGYQMPSVMDPSSSLGGGHGLIQELCLKREREQQQQQQQQFAQPWLSEQQQLEMAGEGAQTMFGTTRMEQEFNASSTPESSTQPVGMSFASFPSSSAAGATASSHMSATALLQKAAQMGATLSRPSCQGQMAASTFSSSSSSAAITNVTNNTPPAATSSSAATSTAVGVGFGHAFEAPAPYGVDQRPSANRNAGNAAAAGGGRGNAGGANDGLTRDFLGLRAFSHGDILSMAGFDPCMPTSASARAAYDQQGPQSSNPWHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSX6</accession>
    <name>A0A8I6XSX6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dehydrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant dehydrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973103.1">
        <property type="nucleotide sequence ID" value="XM_045117168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSX6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305460"/>
    </dbReference>
    <dbReference type="GeneID" id="123440604"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0255060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0255060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0255060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0305460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440604"/>
    <dbReference type="OrthoDB" id="715558at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000167">
        <property type="entry name" value="Dehydrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030513">
        <property type="entry name" value="Dehydrin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33346:SF27">
        <property type="entry name" value="DEHYDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33346">
        <property type="entry name" value="DEHYDRIN XERO 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00257">
        <property type="entry name" value="Dehydrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00315">
        <property type="entry name" value="DEHYDRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00823">
        <property type="entry name" value="DEHYDRIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="29175" checksum="110992A90D53B8BB" modified="2022-05-25" version="1">MEYQGQHGNAANRVDQHGNPVAALGATAVTGPPTGGQLQPGREEHKTRGILHRRSSSSSSSSSEDDGMGGRRKKGIKEKIKEKLPGARKQTYGQPAAPAGMTGAGATGGPHYVQPAPAGTGVHGTTATTGTYVQPAPAGTGAHGTTATTGTYGQPAPAGMTGTGAHGTTATGEKKGMKDKIMEKLPGGHKNGQHTMPSAGAYGQPGMTAAGMDGNTTTGGGYGGQPGHAGTGTHGHATTGGAYDHQGHPGMTGTGAHGTTATGGAYNQQGHAGLAGTGEKKGIMDKIKEKLPGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSX8</accession>
    <name>A0A8I6XSX8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSX8"/>
    <dbReference type="SMR" id="A0A8I6XSX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0333090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0333090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0333090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0400270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF133">
        <property type="entry name" value="OS03G0193150 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57394" checksum="5E75B98005DFAD68" modified="2022-05-25" version="1">MDPGGPSRPAPFYAPFYAGGPAAHGGEAPSSSSPRPPPPPQPQQGHAIQPAPLRQTSRPWDLGAGALPTGFRTPPLHPQFGASSSGRYLSMPVEAGTAARNFERNPRDIPCSLGPSIRPSPWTPSNSDMVAVPQQRYAPQNWPPPSPPPQFVQHPPRFEPLPPQPWGAPEPPIYQDPRVQETKLVLLNSPMVLGQLVMSPEHSAILVHQLEVGDEQMRDMVLDGFKHCGREIMGDTQGHAVLEALLHSLHGRYRGLEIIVEVAIKPRLLSRSKDGVYSLELLITAVAGFPALSARLVHSFVNERVMDDVGGDELIVRCFTTMPDYVTAVLINHAIDTIDDKMLCPFGSTFLATCFEYAEGEEVPRFQTIILAKAVEMAKKRAGKHFLLHLLNHANVSIQFRKQLMSKLMKKVLDLCFHCQGQIVVEQCFVSKETELLNIALRACADLTTGELQVLLGERVVSRSFVLLLLHRLVEDGNTPFPEIAKDLARKIEELPPHIRKHDDVKLLMSAIRRVLARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTZ8</accession>
    <name>A0A8I6XTZ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTZ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0583600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0583600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0583600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0583600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0583600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0583600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF87">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0583600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="525" mass="60169" checksum="88A9D31ABB18947B" modified="2022-05-25" version="1">MTVTLQDVSMITALPIEGKPLCMSTDSEGWRHQMEALIGMSPQEPEVEDGGKKDRVPAGATFTWIAANFAHCPEDADDEVIQRYARVYIWYVISRTIFADGTGKNAPWMWLKALTVLDNKFSWGSAALAYLYRQLDDACRRTTKDGGVGGCMLLLSVWSWERLPVGRPKSSKWNTWNDHDNPVRQPTWAYKWDLVSEVASEVNLLYKQYTNEMDSLTPEQPHPPEWVDTDTQLHRLDRRRQRKIKDWQKHHKSYVLMLEQSVQATSSIQRTQYRQHCALAFSNYLRWFQESTRVEICPPAYEEDILEEPIEYDELSQGGYSKLIRKGYQTSFAPVLNFVRKEVKKQADESEDILDNTPGGKKGESALRLFIKEQGKKLRRLSNILGCRDPEYVSPSRSGSTERNTLDDAASSGPRMDDGDITSAANTQRNNQEDDVDTPHAADDMTLKDFQRSAYMLKPRKVFKRYSPDDYAKGKNPVAGGSRLSRMRSRDDEVEDDDDDESDDPEQFVVARRKKLVSKRGRGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZI9</accession>
    <name>A0A8I6XZI9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZI9"/>
    <dbReference type="SMR" id="A0A8I6XZI9"/>
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        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AP2_ERF"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="43"/>
            <end position="98"/>
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        <location>
            <begin position="158"/>
            <end position="216"/>
        </location>
    </feature>
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        <location>
            <begin position="117"/>
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    </feature>
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        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0560680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="24270" checksum="C0EE676B3C210CDC" modified="2022-05-25" version="1">MWSRCNSEGRRFGGYGRGYGACDLLWPRRDGRSHALPVQSGTGFRGVRLRDSGRYAAEITVDHSPMWLGTFPMPELAAHAYDAVAWQYGRPRSEMNFSDVETAEDATTMAPGFRMVCRAMEEENREARRQLRAAEADEEAMEKLRRENPDLVKTEGEFFASRGEKKPKTEANPFTVAGPSAPMINIEDSDESGLSDSDSDEEFDVDWKTLTRESGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0G2</accession>
    <name>A0A8I6Y0G2_HORVV</name>
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        <recommendedName>
            <fullName evidence="9 12">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123449027</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
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    <dbReference type="GeneID" id="123449027"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0323190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0323190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0388500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0388500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0388500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449027"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000114">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="83"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="553"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="601" mass="66236" checksum="59718561CE60F7B8" modified="2022-05-25" version="1">MGDLEDKLKGFEIKKEGEAEILMLNSNAVFFNPVQVHNRDMSIAVLRTFVAKRKEEHEELMNKRNNKSHKKGNQIETPVVNGDTALTQHDETDVVHEKEPNQATNEIEDPSKEETKTPSGKVTRELKPPIVLEALAASGLRSLRYAREVDGLGKVVALDNDKASVEACKRNIKFNGASVISKVEAHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQAVADGGLLMCTATDLAVLCGTNGEVCYSKYGSYPVRGKYCHEMALRILLASIESHANRYKRYIVPVLSVFMDFYVRVFVRVFTSASEIKNAPLKLSYVYQCVGCDSFHLQSLGRTVTKNNSVKYAPGIGPVVPQECSDCGKKFNVGGPIYSAPIHDQDWVLSTLTDVRPMKDRYPAYNKITSVLTTVSEELHDIPLFFSLHNISGTVKCTSPSAVMFRSAVINAGYQISSSHCNPLGLKSDAPWDVIWDIMRCWVKNHPIKEQPRDSAGTAILSKSPQLEANFSRAVAALSKAQMKKVNRFLPNPESHWGPKVRAGRRITNKHISLLGAEATNEAISRQDGNGAVTDKPGSDTGATVTDEENEPSTKRQKTDDGEPASEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2M9</accession>
    <name>A0A8I6Y2M9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">glutamate carboxypeptidase II</fullName>
            <ecNumber evidence="18">3.4.17.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC123452609</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="18"/>
    <dbReference type="RefSeq" id="XP_044985227.1">
        <property type="nucleotide sequence ID" value="XM_045129292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2M9"/>
    <dbReference type="SMR" id="A0A8I6Y2M9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517260"/>
    </dbReference>
    <dbReference type="GeneID" id="123452609"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0429840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0429840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0429840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452609"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000164">
        <property type="entry name" value="Os01g0740650 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000034">
        <property type="entry name" value="Probable glutamate carboxypeptidase VP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF75">
        <property type="entry name" value="GLUTAMATE CARBOXYPEPTIDASE AMP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035315590" description="glutamate carboxypeptidase II" evidence="19">
        <location>
            <begin position="38"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="21">
        <location>
            <begin position="326"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="20">
        <location>
            <begin position="580"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="727" mass="78202" checksum="0AE7B42AA24D42BE" modified="2022-05-25" version="1">MPHPSSSPSSPPQPLRLARVLLPFGLLLLVSLLVLHGRPPGARSGADPAALFLSLSPGANASIAADLRALTAGPHLAGTPGAAGVAAHVHARFRAAGLRTLTREYAPLLSYRAHASLALLAADRSLLARLSTDEPADPGRRVVRPYHAYAPSGAAVAEAVFVNLGREEDLAALDRLGVSLRGRVAVAIRGGGYRGGVVARAAGKGAVAVLIAGHADGGVERGAVLLGGPGDPLTPGWAAADGAERLGFDHEAVRRRFPTIPSMPVSADTAAAIVRSLGGPALPPEWRAGLGLGLGLEGHAGGVGPGPTLVNFTYQEDRKMATIKDIFAIIKGYQEPDRYVILGNHRDAWTYGAVDPNSGTAALLDVACRLGIMLRSGWTPRRTIILSSWDAEEFGMIGSTEWVEENLGDLQSKAVAYLNVDCAVQGMGLFAGSTPQLDKLLIDVTRQVKDPDVEGKTVHDTWSTMNGGINIERLARTDSDFAPFLHHAGIPCVDLYYGKEFPGYHTALDSYNWMEKHGDPLFLRHLAITEIWGLLALRLADDLVLPFDYQTYASQLQEHTNVLADMVNNSQSVSIINGFINDLSSAATEVQKEAKELKLLDIHDGHDLMRRRLLNDRLMLAERSFLHAEGLQGRTWYKHLLYSPPEDYESELSFFPGIADAISRSGKQSAKQRQAVVRQELWRVTMAIQRAANVLRGEFGAHNKPINFRGEFGAHNKPINFSVSVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3N4</accession>
    <name>A0A8I6Y3N4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123449548</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate biosynthesis; Calvin cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 11"/>
    <dbReference type="RefSeq" id="XP_044982738.1">
        <property type="nucleotide sequence ID" value="XM_045126803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3N4"/>
    <dbReference type="SMR" id="A0A8I6Y3N4"/>
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        </source>
    </evidence>
    <sequence length="231" mass="24739" checksum="A717CED57306FDB1" modified="2022-05-25" version="1">MGKGKVHPSPPAAAAGETAEGVLLRLLPAVVLAMAAPLGPEGKEVLAYLVLASLRSSAPPTPAREELVEGCGRKPHRPELGCGCFGCYTAYWSRWDGSPERDREAIHRAIEAFDEHLARKEEGKGVRRRKKRAASSKGKAKAPAAVDPPLPEQGEASAAAARVPAEVVDGGEEEVRAVEEEEATTAACARDDVPEERRRRVWGAVAGVFNWRGWGLWGSHSSVTQADIPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5L1</accession>
    <name>A0A8I6Y5L1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exportin-5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0259780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0259780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0259780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0311070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF18">
        <property type="entry name" value="EXPORTIN-5 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="1">
        <location>
            <begin position="241"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="1">
        <location>
            <begin position="724"/>
            <end position="1130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1641" mass="186818" checksum="2F79D92529082987" modified="2022-05-25" version="1">MRQLGEELYEDLLAKASLELLGKGLPPQVRHHGFKMMRHLSRSRSTAAALVAKVIWSGGISLLNDLLSPVAYLSKPGPDDAELAAFTLKWISDFRGGLCEADLPDLDEFLTNVMLLLSSLLEKHFESIIAERRDELMVSTEKDVLTVTSCLIAANAYAEWVPVVHLVKHGLVKRCESLLHLSKFQTYALKFFKILCQRRRPITVAKDYDDAMGHVFEILMSISQDSLTKVRACSDFNSERALDVAKGICDCLVTLVSSNMQCIIADSIKTSHFLRRMLEYYQHYKIALHFQCLPFWLMVLSEGPKDFFVASDPADVASASGTNSAENANIGTFLLFSDDICSVILDVSFKRMLKTPATILPYSLELWSDELHGKNGFIQYRSRLLDLIKYIAYRRPILAASRAAQRINSVTGDASTVSPPPKDLAAIESAQLVLNTVVNAIFDDSVENGEIVLDTQFSLHRIHHIFGGLLAQLLSLKWTEPRLAVILGHYLNAFCPYLRCHPPVVPSVVNKLFELLPSITASSHDLLTAQSARSQICSCIIRVCKAVDSTALPSDMKDFFSPKITEEDVLAFGMLDRANYSIEQNKGGDWSQDGHTIDLSASSNTMSSRQIHVQTARLGPCALKDENTGLSVAHPYLEYPLIIQPSPDSCFKPSCRKANPFTSLSVKQVVSGTDEPGLEALAVNNFDDQEYVPVQGNANSDLERPIESFGPKCLGKFKVTQGYQGKAKLLLWEHNLLSEAFISVTACPGIECKKQLLMSLLKSLNKIWTQPEWDEEYMSYTYRLCGLLCNDQFMETVLLLVKSFEEIKGSKIKHSTGTQEKCSPTSTANYRQSSTFLQLMLTLLLRILHFIHMAWTDQSAYDLPEILRRAKLMSTMEISTFLAETNTLPDNDGVDAIRVDAIATRLRKIRETGYNVIGLCANVGRSFFELDSSLFIHALVADISSMEFDHLGKLIQLTFVPLVRCCPRECWDKWVLLLLEFLFFYCERNFRYAWLTLINEGRAKVPDFFGDLDGPEEKLKKLEVELLLKFTRSVSLLLRVLASKELNSGLPDLNCPKSDLKSISSSSLMGYLLLNNCFGSFSMYLFGCLVDYQAATQALPFFHALIRLAVATDDGRLKQFILNEMLPTIVRFSDKSPQSGISRLRSDLSSSNAVSSMDDVVCLGQEIYEVYLHNQVTMMNGERADIKTRADGFIDWLSNELKSLHYRASLPAPNIFPKHSVWNWEFNEEFDRYFPTYMEMLHEVDTMNDCLEHKYLNGDALLERLNPEFKAKYAINSLQHPYLQIMSRMLPRKERDVCCRRRTDEIYKFLVQLINLKPYIKQNDCPDDVMDRLEESCHPQSDLWEVYALRAVDIFLDWLLVFWEPQFHPLIREHHNHKELLLTSARELAEGCAVFQPLKPDPADFLAHLRPYASVYIKTRKEQSGYYTAKKQVRLHEEFDNYLATCTWDHAIDFHSFMESARRDDEDAQFAELDADLAKMSFERKAKIVKRGHEMNSYTQKLRDLLLNDKVKDQLASLMDLLDEEGFFSTDDSSIDWAKESFTKLVEKFNDLTENCFDGCYAIQGIMDLQKFLPRKDDTCHDTVQMADLLLKVDHANKVWKGNLEHFWRDTRHYEHGYYNMLRQPLEEVFKSSEFVAMGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7R6</accession>
    <name>A0A8I6Y7R6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7R6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0548520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0548520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0548520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0548520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0548520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0548520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0548520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23144" checksum="66692DB1272B305E" modified="2022-05-25" version="1">MSLPCSDQSIRPKKMQSASFPRGVEAVRCWCGDLCKVKEVTDFSDWLGMKFFMCANYESDPPESICAYLRPLSPPPLCMYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERRAKAEAKEKAEQKKEWKDYCVEQRAFIDEMIRKNQEEKLLLGEVYRQREQAREVERERKRERARAAKAAQEAGDGTGKYPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y847</accession>
    <name>A0A8I6Y847_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y847"/>
    <dbReference type="SMR" id="A0A8I6Y847"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0389400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0389400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0389400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="26941" checksum="B606BFF25AC01C86" modified="2022-05-25" version="1">MATFIPVKFTEFCQPDATMPFRKELVQITTDLRIALPTITGKPTSSYPEDSRYRIEVHVPGRTFQPRTEPMYFKFIAPNWILGRDMAIHCALGRIKEEHKGCPISLDLVTVSRRSEDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLRELELEEAAREARYEHDLEAKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPVISNDEDYEESSTEGPTAMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8L8</accession>
    <name>A0A8I6Y8L8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123401980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951779.1">
        <property type="nucleotide sequence ID" value="XM_045095844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y8L8"/>
    <dbReference type="SMR" id="A0A8I6Y8L8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553340"/>
    </dbReference>
    <dbReference type="GeneID" id="123401980"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0459680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0459680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0459680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0553340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0553340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0553340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401980"/>
    <dbReference type="OrthoDB" id="1717827at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF59">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="59237" checksum="166A71DC69B21CD5" modified="2022-05-25" version="1">MPKKTSTNPMLRTAARRRISVAPARFAHGLAQAQDQDQPAVHERGGLEPSWVPLYRRISKLSNGRQPGMAAAEMTKYLRKRRPLSEYQIVAYVRKLRKFKRNACALELMEWMEARGAKLTPGHQALRLDLVSKVHGIQAAEEYFWSLPDILKSRKACSCLLNCYGEHMMACKGLEFYEKMKATNIVPNTLVYNSLMALYKKAGQPEKIPSTFEEMRERGITANSFTYFTLVESYVTMNDLEAAEKVLEELQKVAPLHWSLYTLMANSYVKLELCGKAEIALKKAEEVMNKAELSSWYFLLSIYARCGNATEVKRIWESLKSTFKKCLNRSYLVMLQALSTIDDFESLQEIFQEWESSHEQYDMRITNVMIKAYLDKGMIDEAEAIRQRTMAKGRCDERTIYIFAEYYLEKSNVTAALEILRDAKKMVTAHKWVPSKKHRSRFLKHYEESKDVDGVESFCECLRKLECLDVDAYEGMMRTYIAAGRTNPSIAQRIEDDGIHVGPETTKLLERVSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIZ1</accession>
    <name>A0A8I6YIZ1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIZ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0591260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0591260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0591260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326:SF54">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326">
        <property type="entry name" value="OS05G0543800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3615" evidence="2">
        <location>
            <begin position="150"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="271" mass="30823" checksum="73204A555B420B78" modified="2022-05-25" version="1">MAGDRYPSPPAGSQASSSSETETGVPEHVVEVSSSELGGASNDLTKFETLMLNDTPHTPESADGQEIFGGFYPLNLQGDIKFKRRQAEERAQFFQAQGYWPLDFETNREYEGMKLGKQTQSVQARGQHTYASKKEEIAANAKRWMSEEAMAAFTRYIESKDDLKGLDYEFDELVHQCFNVADYLKIFHHFNFTVKIKKPGSADWTSAMYFAEVKSICMIKVYACYPLEQDQYGECYACKNQGMGELRHPLKGIYDMGSEDIQHPFYYEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YM86</accession>
    <name>A0A8I6YM86_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YM86"/>
    <dbReference type="SMR" id="A0A8I6YM86"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0684710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0684710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0684710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0568430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0568430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0568430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0684710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0684710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0684710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF34">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035309764" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="9">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0684710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="358" mass="36292" checksum="CAEE9EB0B0546576" modified="2022-05-25" version="1">MASAAALLAMVVLLACLALADPPAREQLALLAILAATPLERKLGWSASTPACAWVGVTCDAANSTVIKLRLPGVGLVGPIPPSTIGRLTNLQVLSLRANRVSGAIPDDILRLSALRSVFLQDNAISGAILPGVSGLAALERLVLSHSNLSGPIPFALRGLAALRALRIDGNRLSGKIPSIANPGLNTTGSTAPSRRRSRGMGPSDGKPPKKKKKVSTAAIVGIIMAAVVVALLLLAILFCCKRSRRGARTDGAKGTAAAATGTTRPPASSGDGTGTGNDAGTSGSVAAAAAGGGTGEASRLMFVGKGAGYNFDLEDLLRVSAEVLGKGSVASERGRGERGEEGYRGERGDQEEAGRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YM98</accession>
    <name>A0A8I6YM98_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123452671</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985312.1">
        <property type="nucleotide sequence ID" value="XM_045129377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YM98"/>
    <dbReference type="SMR" id="A0A8I6YM98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518380"/>
    </dbReference>
    <dbReference type="GeneID" id="123452671"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0430810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0430810.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0430810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0518380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452671"/>
    <dbReference type="OrthoDB" id="10249567at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF481">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="18"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="181"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39655" checksum="15E14EB753F649FF" modified="2022-05-25" version="1">MAEHCREIPAAIIADSEERSYVLKVDGYSRTKALLKNGECMTSTPFSVGGHIWVVRYYPNGYPKHCDDFISLYVHNESADAEDVKVKAKLKLSVLDQNGTPVPSYSRAIPLHTFSSKAPDRGYHDFIHKAGLEGSPHLRDDCLTIRCDVTVVKDILDEEARIPPSDLHRHFGDLLGSKDGADLTFQVGGRGFPAHRCVLAARSSVFKAGLLGAMAESSCSTIEIRDMEPDVFECLLHFIYTDSVPLLDVVMAGHLLEAADRYDIPRLKVICEEKLCSHIDSNMVATSLALAEQHRFHRLKEACLRFLVTPSNFKAMIASDGFDHLTSSCCWSVVEELTARIIRLNSAEDIIMTFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQS5</accession>
    <name>A0A8I6YQS5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQS5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0714450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0714450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0714450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0714450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0714450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0714450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="6"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0714450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="123" mass="14955" checksum="0F26CD3B3572BE3E" modified="2022-05-25" version="1">MCTRDSNIWRSRCPMICFYAVEFHFVDRVATQFGKRQGIPTEETRSVITSAHGFSRRNNQDISDWAVKHHHWIAMWNQRETLIHKENRPHNDSAYQKYLVWYADRYRLKLKPGWTREEWSELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRN7</accession>
    <name>A0A8I6YRN7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953010.1">
        <property type="nucleotide sequence ID" value="XM_045097075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YYQ5"/>
    <dbReference type="SMR" id="A0A8I6YYQ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0625190"/>
    </dbReference>
    <dbReference type="GeneID" id="123403108"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0518760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0518760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0518760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0625190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0625190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403108"/>
    <dbReference type="OrthoDB" id="742397at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008709">
        <property type="entry name" value="Neurochondrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13109">
        <property type="entry name" value="NEUROCHONDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13109:SF7">
        <property type="entry name" value="NEUROCHONDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05536">
        <property type="entry name" value="Neurochondrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="69879" checksum="E13DF6F9CABD96C5" modified="2022-05-25" version="1">MDSSPVATTPSAPTAASAPPLEDCLRLLRGERDEQKLAGLLIAANVCRAGDAAAVAQVYRAVGPRFLRRLLNTGLGKVEGGKEEEREAYLRLAVTVLAGLARAPEVAADAGVVSAVPLIAEVVSKSPDLTITEECFELLSLVAIASEDGAYKFCEPGVMDMLFSQISNFPEGSRCLELSTHLLQLLVHKLRADSMSVEKLQGMASMVTSLAMLFGVLHTAVKFESLHMLATLLSQKESPLHDALRSMPSTIWKSHIRGGIIDVLQNRVVSSEKLQALLLAECMMSILGENWLSEDHKILDDKNAISVDKFVLLVLQSARVEVAVLLNELAFSKYESSKSSQTDDAIIQKQRNLAILFSLIERIIKMISDASSGEGEPSQTICERTIMQVITGLNETIGLVLDFLQDAKDHGQRKGDDLLAAVRIVGSYLAETPYACQEKTGHLLEFIFSIEGQDESSPFYSVRFMLPMLSQITTTADGCRTLVSFGGYKAVIDCLIKMTEENGMMIDDGSMFLACDTIINIMSNGKNYPIKMEPCFIRLLQALITWAGTTDASSVIMTAASLCTMVMELTSEEFLLSCSGFDPKTLGRLSDLIARSLHQDIPDDDSEQLNQKQIIASGYRRWADRFPSVRNVVHEHVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZN2</accession>
    <name>A0A8I6YZN2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123404214</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044954066.1">
        <property type="nucleotide sequence ID" value="XM_045098131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZN2"/>
    <dbReference type="SMR" id="A0A8I6YZN2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556470"/>
    </dbReference>
    <dbReference type="GeneID" id="123404214"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0462220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0462220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0462220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0556470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404214"/>
    <dbReference type="OMA" id="PRATGHW"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001143">
        <property type="entry name" value="Pre-mRNA-splicing factor rse-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000041">
        <property type="entry name" value="Splicing factor 3b subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="8">
        <location>
            <begin position="20"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="9">
        <location>
            <begin position="453"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="7">
        <location>
            <begin position="874"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="830"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1227" mass="135841" checksum="873847569F2F4DE7" modified="2022-05-25" version="1">MYLYSLTLQRATGAVCAVIGSFSGRDSKKSNAAGSSTQEIAVARGGTLDLLRPDPETGRLRTLLSVDVFGAIRSLAQFRLTGATKDYLVVGSDSGRLVILEYSPDRNRFDKVHQETFGKSGCRRIVPGQLLSVDPKGRALCIAALEKQKLVYVLNRDASARLTISSPLEAHKSHTLTFALTALDCGFDNPVFGAIELEYGDSDRDPTGQAASHAQKLLTFYELDLGLNHVSRKVSEPIDNGANMLVTVPGGADGPSGLLVCCDNFVLYRNQGHPEVRAVIPRRVDLPAERGVLIVAAATHRQKSLFFFLLQTEYGDIFKVDLDHHGDTVSELRIKYFDTIPVASAICVLRSGFLFAASEFGNHALYQFRDIGRDVDVESSSATLMETEEGFQPVFFQPRALKNLVRIDEIESLMPIMDMRIANLFDEETPQVYTACGRGSRSTLRILRPGLAISEMARSMLPAEPIAVWTVKKNINDMFDAYIVVSFANVTLVLSIGETIEEVSDSHFLDTTHSLAVSLLGEDSLMQVHPNGIRHIREDGRVNEWRTPGKKTITKVGSNRLQVVIALSGGELIYFEMDMTGQLMEVEKQDMSGDVACLAIAPVPEGRQRSRFLAVGSYDNTIRILSLDPDDCLQPLSVQSVSSAPESLLFLEVQASVGGEDGADYPANLFLNAGLQNGVLFRTNVDMVTGQLSDTRSRFLGLRPPKLFPCIVSHRQAMLCLSSRPWLGYIHQGHFLLTPLSCDTLESAASFSSDQCSEGVVAVAGDALRIFTIERLGETFNETSIPLRYTPRKFVILPKKKYLAVIESDKGAFSAEQREAAKKECLEAAGSAENGNGNGDQMENGDGQEDGEESNTLPDEQYGYPKAESEKWVSCIRILDPRSRDTTCLLELQDNEAAVSICTVNFHDKEHGTLLAVGTTKGLQFWPKRSLASGFIHIYKFVNEGKSLELLHKTQVEEVPLSLCQFQGRLLAGVGSVLRLYDLGKRKLLRKCENKLFPRTIVSIHTYRDRIYVGDMQESFHYCKYRRDENQLYIFADDSVPRWLTAANHIDFDTMAGADKFGNIYFARLPQDLSDEIEEDPTGGKIKWEQGKLNGAPNKVEEIVQFHVGDVVTCLQKASLIPGGGECLIYGTVMGSVGALLAFTSREDVDFFSHLEMHLRQEHPPLCGRDHMAYRSAYFPVKDVIDGDLCEQYPSLPADMQRKIADELDRTPGEILKKLEDIRNKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z2T2</accession>
    <name>A0A8I6Z2T2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z2T2"/>
    <dbReference type="SMR" id="A0A8I6Z2T2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0666570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0666570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0666570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0666570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0666570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0666570"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044607">
        <property type="entry name" value="RKD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373:SF28">
        <property type="entry name" value="OS06G0228950 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373">
        <property type="entry name" value="PROTEIN RKD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="7">
        <location>
            <begin position="110"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0666570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="250" mass="28220" checksum="5279AC200E8784CB" modified="2022-05-25" version="1">MPPASGLMGEVFGMIVTCLKRRRSSAVYPSRASVANHCALSTLLFRRAAVCKSSRAAPCLVPCNTGDLDTSFEDDLLRCWEAMEQSDNSTEVSGKGLPLPLPCYGEENGSASNATRADRVRSERVLTFELVSQYFYMPITQAARELNVGLTILKKRCRELGIPRWPHRKLKSLRTLINDVEVLQEAGKADDDGQLRALVEMLEQERRLLEQKPYVELEEKTKRLRQACFKASYKKRRLLALEPGEASKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1H5</accession>
    <name>A0A8I7B1H5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123416995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962788.1">
        <property type="nucleotide sequence ID" value="XM_045106853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1H5"/>
    <dbReference type="SMR" id="A0A8I7B1H5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079460"/>
    </dbReference>
    <dbReference type="GeneID" id="123416995"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0064740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064740.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0079460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123416995"/>
    <dbReference type="OrthoDB" id="663499at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000466">
        <property type="entry name" value="MAP kinase kinase kinase18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF98">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="49"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079460.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="493" mass="52088" checksum="30EB01EFB153827B" modified="2022-05-25" version="1">MHRHPPKNNPSTIQLPASKLEIRVPSQQTTKIPIPNAEEKATMAVSKQWTRVRTLGRGASGAEVFLAADDASGELFAVKSASTACAAALRREQRVMAGLRSPRVVSCIGGRGDRDGSYQLFLEFAPGGSLADRVASNGGLDELAIRGYAADIASGLAYLHSAGMVHGDVKARNVVIGADGRAKLADFGCAREAAAGAPIIGGTPAFMAPEVARGEEQGPAADVWALGCTVVEMATGRAPWTGMDGNALAALHRIGYTEAVPEVPQWLSAEAKDFLRGCLVRQAGKRCTAAQLLEHAFLASAVVDGKPQAVESKWVSPKSTLDAAFWESESDTEEAEHDSTAEMRIMALACPASALPDWDSDEGWIDVLSAPTEAPVAVEATTGLDDEAIRNEEGSGAGSRVLASTSDVEHNGDNGFLNAAEASNDSVRDHESLEECFASHQPVSCKLLLLLSTNSTSVLQHKSIDFVPCTNSVLLRAAPLFLRSYFCAPLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3N5</accession>
    <name>A0A8I7B3N5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine racemase</fullName>
            <ecNumber evidence="9">4.3.1.18</ecNumber>
            <ecNumber evidence="10">5.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">D-serine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123430675</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the synthesis of D-serine from L-serine. Has dehydratase activity towards both L-serine and D-serine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="9"/>
    <dbReference type="EC" id="5.1.1.18" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044970458.1">
        <property type="nucleotide sequence ID" value="XM_045114523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3N5"/>
    <dbReference type="SMR" id="A0A8I7B3N5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188890"/>
    </dbReference>
    <dbReference type="GeneID" id="123430675"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0156420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0156420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0156420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0188890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430675"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030378">
        <property type="term" value="F:serine racemase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070179">
        <property type="term" value="P:D-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006563">
        <property type="term" value="P:L-serine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000005">
        <property type="entry name" value="Threonine dehydratase catabolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050">
        <property type="entry name" value="SERINE / THREONINE RACEMASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050:SF1">
        <property type="entry name" value="SERINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0702">S-nitrosylation</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="31"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="337" mass="35760" checksum="E0D958AA73DF280F" modified="2022-05-25" version="1">MGSRDDDGHITECQGYAADINSIREARARIAPYVHKTPILSSTSIDAIAGKQLFFKCECFQKAGAFKIRGASNSIFALDDSQAAKGVVTHSSGNHAAAVALAAKLRGIPAYIVIPKNAPACKVENVRRYGGQVIWSDVTMESRESIAKKVQEETGAILIHPFNDKYTISGQGTVCLELLEQVPEIDTIIVPISGGGLISGVTLAAKAINPSIRILAAEPKGADDSAQSKAAGRIIKLPATSTIADGLRAFLGDLTWPVVRDLVDDVIVVDDNAIVDAMKMCYETLKVAVEPSGAIGLAAALSDEFKQSSAWHESSKIGIIVSGGNVDLRVLWDSLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5H5</accession>
    <name>A0A8I7B5H5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123443993</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976518.1">
        <property type="nucleotide sequence ID" value="XM_045120583.1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276480"/>
    </dbReference>
    <dbReference type="GeneID" id="123443993"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0276480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443993"/>
    <dbReference type="OrthoDB" id="509637at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053290">
        <property type="entry name" value="TSET_complex_member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45521:SF2">
        <property type="entry name" value="TRANSDUCIN_WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45521">
        <property type="entry name" value="TSET COMPLEX MEMBER TSTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="259"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1275"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1359"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1368" mass="146102" checksum="DCC2C8497DD6AFD7" modified="2022-05-25" version="1">MLRLRAFRPTSDKVVKIQLHPTHPWLVTADANDRVSVWDWEHRQVIYELKAGGVDERRLVGVKLEKLAEGETDSKGKPTEAIRGGSVKQVSFYDDDVRFWQHWRNCSAASEAPTAVNQQSSAFSAPAPSTRGRHFVVICCENKAIFLDLVTMRGRDVPKQELDNKSLLCMEFLSRSSSSDAPLVAFGASDGVIRVLSMMTWKLVRRYTGGHKGAISCLMTFMSAAGEVHLVSGGSDGLLVLWSADHIHDSRELVPKISLKAHDGGVVAVELSRVMGSAPQLITIGADKTLAIWDTVTFKEIRRIKPVPKLACHSVASWCHPRAPNLDILTCVKDSHIWAIEHPTYSALTRPLCELSSLVPPQALAQHKKLRVYCMVAHPLQPHLVATGTNIGIILSEFDPRALPAVAPLPALTESKEHSAVYIVERELKLLNFQLSNTANPSLGSASETGRSRNETIDQLIVKQSKKHISTPAPHDSYSILSASSSGKYVAVVWPDIPSFVVYKASDWSVVDSGTGKLFAWDSCRDRYALVESALAPRMPLIVKGGSSKKAKEAAAAAAQAAAAAASAASTATVQVRILLDDGTAHVLQRSIDGRSEPVVGLHGGALLGVTYRTSRRISPLTATAISTVQSMPLSGFGGSGSSFASDDPFSSREGPPQNFQLYSWETYQPVSGLLAQPEWTVWDQTVEYCAFAYQQYIVISSLRPQFRYLGDVSIPSATGAVWHRRQLFVATPTTIECVFVDAGVAAIDIETKKRKEEMKAREAQGRAVADHGDLALITVEGPQVTASEKISLRPSMLQVVRLASFQHAPSIPPFIVPKQSKLNGDDSVFLKELDDRRYSEVAVAGGGVSVAVTRFPSEQKRPIGPLVLVGVRDGVLWLVDRYMCAHALSLSHPGIRCRCLAAYGDPVSAVKWATRLGREHHDDLAQFMLGMGYATEALHLPGISKRLEFDLAMQSKDLKRALACLLTMSNSRDVGQETTATDVTQILNLAVAKQAKQESLADAVQGIVKFVKEFFDLIDAADATGQAEIAREVLKRLAAAASVKGALHGQTLRGLALRLANHGELTRLSGLVANLITAGHGREAAFAAAVLGDNALMEKAWQDTGMLAEAVLHAQAHGRPSLRSSVITWNKMLQKELDHTPTVKTDAAAAFLASLEDPKLTSLGETEKKPPIEILPPGMPPLSAPPIIIKKAAAKPGLANASQTLNAGAPMNQGTPMVQGTPMNQSTPMAQGTPMNQGTPMAQATPLAQGPPAPAQSTDEVKPSEATAAPDNVEATAATSNAEATEAPGTAEATEASVPDPTSSSPDAAAAAPAPAPAADSSGTDALAVTPSDSTDDAPSSTKVPETQDKPPSTEASPSPPPSISAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAV4</accession>
    <name>A0A8I7BAV4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAV4"/>
    <dbReference type="SMR" id="A0A8I7BAV4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0542550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF218">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="319"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="660" mass="73531" checksum="1216AE233241A925" modified="2022-05-25" version="1">MFQYEYTGPAPYYCDCDGGYGGNPYITDGCSRDKGYDPKQRKTNCSRRCGQINVPFPFGLEHGCSARMSFQLNCTNSSLSTLQFPDVDGKYVKYINIMEGLVNVKYSIYDEEDISVRVPQDPGHYVASAGVVPQQWAVANLTCPEAKVNKTGYACVSMNSKCLDVNSELEYYGYRCTCMAGFDGNPYVSNGCKDVDECNAPGTCNGTCHNTKGGHYCTECPSNTVYDTALKMCVSTKKQNLVLGIAIGISVGFGIILLMSIVILLIRRWKKDIKKKLRRKHFRQNQGLLLEQLISSDENASDNTKIFSLSELEKATNDFDPTRIVGRGGHGMVYKGILSDQRVVAIKKSKVIEQVELSQFINEVAVLSQVNHRNIVKLLGCCLETEVPLLVYDFIPNGSLFGILHASTTSSSIFSWDDCLKIAAEAAGALYYLHSAASVSIFHRDVKSTNILLDGNYTAKVSDFGASRLVPIDQTHVVTNIQGTFGYLDPEYYHTGMLNEKSDVYSFGVVLVELLLRKKPIFTSDSGLTQNLSNYFLWEMREKPLAEIVATQVLEEATNEEINDIATLAETCLQLRGEERPTMKQVEMKLQYVRSKRLRSSQVVLKNEDMQPLLSGQSQDTLPMLTTPSGRVDRSNIDSQRNQNCYSLEQEFMASATLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAZ4</accession>
    <name>A0A8I7BAZ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123404925</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for autophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATG5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044954769.1">
        <property type="nucleotide sequence ID" value="XM_045098834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAZ4"/>
    <dbReference type="SMR" id="A0A8I7BAZ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546660"/>
    </dbReference>
    <dbReference type="GeneID" id="123404925"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0454390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0454390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0454390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0546660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0546660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404925"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Autophagy protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000370">
        <property type="entry name" value="Autophagy protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="3 5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="9">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="8">
        <location>
            <begin position="122"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="7">
        <location>
            <begin position="212"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="277"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="364" mass="40653" checksum="7C662380A025131D" modified="2022-05-25" version="1">MATAAAWSEEAARLVWGGAVPLQVHLHDADVTALPPPPPFLTLGPRIGYFPLLVSTIKAHFSSSLPPGVDTVWFEYKGLPLKWYIPIGVLFDLLCADPERPWNLTVHFRGYPADILSPCEGEDSVKWNYNNSLKEAAFIITGNSKNVMNMSQADQLAMWESVRKGDLDSYMNISTKLKLGPFEEDFLVRTSSLEPRQGSDEPESPGSVKPCRVPVRLYVRRVQQDLEYLEDAIPVSDWESVSYINRPFEIRKEGGRSYIALEHALETLLPEFFSSKPAARAADPEPAATTPDSEPKDSDTSPSTHHDEKPPPASPQETDVAKKTKLKLVRVQGIELDMDIPFLWVANNLKNPERFLHVCVYVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BBY4</accession>
    <name>A0A8I7BBY4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BBY4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0591540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0591540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0591540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0490290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0490290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0490290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0591540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0591540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0591540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47127">
        <property type="entry name" value="10A19I.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0591540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="301" mass="33119" checksum="BE514A1F9D4613D4" modified="2022-05-25" version="1">MGAHGGHGGGNHPLRWTSPMFGFMLRRFVELIASGVKTDKGFKEVHLNQVAKNCSDHFGLAITGTQVYNHLRKWRARWVKISKLRDISGSLWDDTNCVISLEEEHYLGHIKDHPKDVEYLNVPLENYVQMLAIFGTGIATGRYAMASNEALGVASMAGTSPSVVNIEGSGFEFVVDGNEPDSSATAAAHGEAAVAHSTGKRKRASLMSEEEVLVMTNMSEAVCEVANAIKSTTEAHPELYDAVMELPGFSEDDLLIILDYLNESANRARSYSFVQMTETRRTRWVIHHLSKLKDDVPKGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BC23</accession>
    <name>A0A8I7BC23_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC23"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0597580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0597580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0597580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0495320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0495320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0495320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0597580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0597580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0597580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
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    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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    </dbReference>
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    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="150"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0597580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41657" checksum="6032F383574A0BBA" modified="2022-05-25" version="1">MKFNSGRRHRPNLKTEVGSGDMLSILPGNIQACNGDRLSELPVDVLLNILERVGTLDAIRTCILSKQMHMLPTMLSQIVIDLSPRDLFQKSDVVADVTNKILSRRCPHITIRMLKLKFVLSPSCCLSIGKSVGLAMTTQKFDAAEFQIMTPKDFHVSTDAHRVLFAKQFNTFVHDCPDAFAGLTRLHLQNMNFGESDIPNILSYCKRLESLSFFMCGAGSSSVMYLEHAQLVELVISYCRFKTVEVKSLPKLQRMTFGDWTCDENPLVLNFVPQLSKLSLANPNFSGKTLNLSKLLANAPTVNDFFLEFRSEKIWVQPECPKVLAPLLAELRFVNLDHLREECDISWTMFLLEAAPSVEDLCITVWDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCB0</accession>
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        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I7BCB0"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="89" mass="9960" checksum="5BE6A509E56F6328" modified="2022-05-25" version="1">MSAPEILQFFTAVDCYPNVSVAYRILLTVPVTIASAERSFSKLKLLKNCLRSTMSQDRLNGLAMCCIEKDVLDKIDLDTLINDFASRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDC6</accession>
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        <recommendedName>
            <fullName evidence="5">DUF569 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC123407221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
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                <person name="Brown J.W."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045100304.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I7BDC6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654740"/>
    </dbReference>
    <dbReference type="GeneID" id="123407221"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0543230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0543230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0654740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407221"/>
    <dbReference type="OrthoDB" id="581309at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="CDD" id="cd23340">
        <property type="entry name" value="beta-trefoil_FSCN_ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007679">
        <property type="entry name" value="DUF569"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054726">
        <property type="entry name" value="Ubiq_DUF569-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205">
        <property type="entry name" value="ACTIN CROSS-LINKING PROTEIN (DUF569)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205:SF62">
        <property type="entry name" value="DUF569 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04601">
        <property type="entry name" value="DUF569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22932">
        <property type="entry name" value="Ubiq_DUF_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF569" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="DUF569" evidence="2">
        <location>
            <begin position="183"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32206" checksum="D8A4293CD001A24E" modified="2022-05-25" version="1">MDRFQDGHHVWLRSRVHGTYLNANSDGKSVSLRGRRASLKAAWTVHIYHGEGDAVYLLLHSAAYGRYLAATARRAPLGLGHNGFRAEQRDYDEPEVQAIMWQAVGAGGSGDDVMLRNVDGRYLRANGRYLRWNAGVSVEDMDRVSTMMYWTVEPIPARESGMHDLPVMPNPFLYVLLSQGISRRQIVFVRADAEGLYDEHGWHMFRFLGRSVNYLRSELVGRIAFIDGQDPGGIAMCVRAGRYGRLTPLVVDLPRYAGHDEAHRIVVMMAGTPAYDELRYPDVNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFD9</accession>
    <name>A0A8I7BFD9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFD9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0735080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0735080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0609750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0609750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0609750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0735080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0735080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0735080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33286">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33286:SF55">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR_PLANT LIPID TRANSFER PROTEIN_SEED STORAGE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0735080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="82" mass="9193" checksum="600B3D4239B15C79" modified="2022-05-25" version="1">MRDCWHNIEKRLGDRYPPLESLCCHTIKDAKDIRCVCDRFTAHELTLISLSKFATVTRACGNGLHTHSNFAEITPPSPPASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFK3</accession>
    <name>A0A8I7BFK3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123452700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044985343.1">
        <property type="nucleotide sequence ID" value="XM_045129408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFK3"/>
    <dbReference type="SMR" id="A0A8I7BFK3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519020"/>
    </dbReference>
    <dbReference type="GeneID" id="123452700"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452700"/>
    <dbReference type="OrthoDB" id="666391at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF309">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="8"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="679" mass="76338" checksum="324FCF21359DA7F2" modified="2022-05-25" version="1">MPPQSSPWPELCRDVLEQVFILLPPHDPGSLIRASLVCRPWRAFLTSPGFARRYREFHRTPPLLGFFENHDTVMPWFAPSSPSASPFPPLFPDRRNLYVLDSRHALVLLRNPIRTDVGDHAVSLIVWDPVGRRQWEFPPPDFASTVLYDVGVVVCAADHCDHLACHGSSFLVGYVGTGYSSAYASVFSSETNSWSPTVSCSHPDIQILICQPKPLVGNAAYSICVSEDEEYDEEETDEDEDVVLRFDLFSRELSKFDGPPIHRNSRYVLMKTEEGVLGCATMQGSRSRLHLWSTETGPDGAVAWMQRRVVDLHKLLPSNTFFSVIGFEDRAGAFYLMTYDGVTTVDLKSGRVKKISIDNSSIVPYISFYTPDQAGTIMHPSTLASSSENVEVAQDEYRDLLLLRSSGQVGDVGECGGWEEVRDKENEGDEEKGEQMEKDVQELCTFGFKAIENKDFSGAVDCQRRILKTRVAHHGKLSPKCCSAYYNYGCALLRKTQSIQDPEDDPENEFWPDLYLAWKMLHVARAISEKSPGSNMEKYKIFAALAKVSVEIDDTDYSLIACFKALAMLEHLLECDHHHIINLNLQVRFAFELESMIGDAKAISLFKSRIENLKRASEDLLADNGDDASFTEVGSEKSFPAKEIRFLTDTLLGALEHKLEDLEQANINPSFRGKCWSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFL2</accession>
    <name>A0A8I7BFL2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123412708</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044961581.1">
        <property type="nucleotide sequence ID" value="XM_045105646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFL2"/>
    <dbReference type="SMR" id="A0A8I7BFL2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742910"/>
    </dbReference>
    <dbReference type="GeneID" id="123412708"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0615990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0615990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0615990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0742910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412708"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000006">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF394">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035339115" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="29"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="493" mass="54177" checksum="1D1F0A830229D1E0" modified="2022-05-25" version="1">MATSSLSWLRVLLLAAAVLASATVGVTARHHRPGFEKIFDAQEADRVEMLPGQTAEVGFRHFSGYVTVNETHGRALFYWFYEAAHDVAKKPLVLWLNGGPGCSSVGYGAMMELGPFLIQKGKPELALNPHSWNKDANMLFLESPAGVGFSYTNTTADLGQFGDNLTAHDAYSFLVNWLDKFPQFKGHDLYIAGESYAGHYVPQLAEKIVHMNKKAARAHKINLKGILIGNAAIDASSDDRGLVDYAWDHAVVSDEVYTAVKNNCMFPDDGVESDACDHAWNDFFNAMNDIDLYSLYTPACTKSMVNSTASSPRRRSKLAGVGTPLGKLHRGGRPYYNAYDPCGDYHIIDYLNRPDVQKALHANVSGAIPYRWEPCSDALTNWTDAPASTLPAIGKLVKDGIRVWVFSGDTDDRVPVTSTRLALKKLGLATKKEWREWFTSDQVGGYTVVYDGLTFVTIRGAGHMVPMITPVQARQVFAHFLTGDELSAKAIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJ98</accession>
    <name>A0A8I7BJ98_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123413084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961965.1">
        <property type="nucleotide sequence ID" value="XM_045106030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJ98"/>
    <dbReference type="SMR" id="A0A8I7BJ98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0699250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0699250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0699250"/>
    </dbReference>
    <dbReference type="GeneID" id="123413084"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0580070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0580070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0580070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0699250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0699250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0699250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413084"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="544"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0699250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="617" mass="70374" checksum="4B33718F36470D26" modified="2022-05-25" version="1">MAYRPYPPPQGPFPPQAPPINPYGQPPQQQAGYGQMPPPPFHAPPPPPPGPPPPHQPQYNFGHARPQQQQQPPPPPQLYYQPPPPPYGGNNNQAPPPPPPISAPPSAPPPPPAQPPPRVAPPPPKEQQAKAALPRVETEEERRARKKREYEKQKVEDRKQQQMMRQSQATILQKTQQVRAAQQQQQPQPQSRHLQPSGGTRVATTVSRPVSAPNTERFENRLKKPTTFLCKHKFRNELPDPSAQLKWLPLNKDKDRYTKYRISSLEKNYLPKMIVPEDLGIPLDLLDMAVYNPPNVQLPLAPEDEELLRDDEVLTPVKPEGIRKKERPTDKGMSWLVKTQYISPLSTDAAKMSITEKQAKERRESREGRDNVLENLNDRQKRIKAIAESFKAAKSRPVHQTKRGMEPEFVLPLVPDFDSYNDPFVMVNFDGDPTADSEQYNKLERSVRDECESQALMKSFQVSGSDPAKQEKFLAYMAPAPHELVKDLDDENEDFQYSWIREYHWEVRGDDKQDPTTYLVSFDDDDAKYLPLPTKLVLQKKKAKEGRSGDEIEHFPVPSRITVSRTAHGDEMEHGESSSMPGNLKRQRSSVDDDLDEHPRHFHVEDMGQYSEEDYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-02-06" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>A1X810</accession>
    <name>A1X810_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">14-3-3E</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123448590</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2007" name="Plant J." volume="49" first="289" last="301">
            <title>14-3-3 adaptor proteins are intermediates in ABA signal transduction during barley seed germination.</title>
            <authorList>
                <person name="Schoonheim P.J."/>
                <person name="Sinnige M.P."/>
                <person name="Casaretto J.A."/>
                <person name="Veiga H."/>
                <person name="Bunney T.D."/>
                <person name="Quatrano R.S."/>
                <person name="de Boer A.H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ295786">
        <property type="protein sequence ID" value="ABC00201.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359144">
        <property type="protein sequence ID" value="BAJ90355.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981478.1">
        <property type="nucleotide sequence ID" value="XM_045125543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A1X810"/>
    <dbReference type="SMR" id="A1X810"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0373780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0373780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0373780"/>
    </dbReference>
    <dbReference type="GeneID" id="123448590"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0311100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0311100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0311100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0373780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0373780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0373780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448590"/>
    <dbReference type="HOGENOM" id="CLU_058290_0_0_1"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="8"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABC00201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ90355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0373780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="266" mass="29437" checksum="B2554776B14F15FC" modified="2007-02-06" version="1">MSPAEPTRDESVYMAKLAEQAERYEEMVEFMERVAKATGGAGPGEELSVEERNLLSVAYKNVIGARRASWRIISSIEQKEEGRGNEAHAATIRSYRTKIEAELAKICDGILALLDSHLVPSAGAAESKVFYLKMKGDYHRYLAEFKSGAERKEAAESTMNAYKAAQDIALADLAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAISELDSLGEESYKDSTLIMQLLRDNLTLWTSDTNEDDVDEIKEAPAPKESGDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-05-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>A4HRC2</accession>
    <name>A4HRC2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NAC transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nac1</name>
        <name evidence="9" type="synonym">LOC123395549</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2007" name="Plant Mol. Biol." volume="65" first="137" last="150">
            <title>The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis.</title>
            <authorList>
                <person name="Jensen M.K."/>
                <person name="Rung J.H."/>
                <person name="Gregersen P.L."/>
                <person name="Gjetting T."/>
                <person name="Fuglsang A.T."/>
                <person name="Hansen M."/>
                <person name="Joenhk N."/>
                <person name="Lyngkjaer M.F."/>
                <person name="Collinge D.B."/>
            </authorList>
            <dbReference type="PubMed" id="17619150"/>
            <dbReference type="DOI" id="10.1007/s11103-007-9204-5"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AM500855">
        <property type="protein sequence ID" value="CAM57979.2"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A4HRC2"/>
    <dbReference type="SMR" id="A4HRC2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0430200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0430200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0430200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0357520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0357520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0357520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0430200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0430200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0430200"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035664_5_1_1"/>
    <dbReference type="OrthoDB" id="744205at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000006">
        <property type="entry name" value="NAC domain-containing protein 100-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF217">
        <property type="entry name" value="OS12G0610600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="13"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAM57979.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0430200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="304" mass="33312" checksum="73765B988A65E505" modified="2007-05-01" version="1">MSMSFLSMVETELPPGFRFHPRDDELICDYLAPKVTGKVGFSGRRPPMVDVDLNKVEPWDLPVTASVGGKEWYFYSLKDRKYATGQRTNRATVSGYWKATGKDRVVARRGALVGMRKTLVFYQGRAPKGRKTEWVMHEYRLEGAHEQASKEDWVLCRVICKKKSGVGATPRPRNLTNIVHGTSTDTSSPPLPPLMDTTLAQLHATMNTSAAATAALEQVPCFSSFSNNSASNSSYLPMVTGNGNGMSYLDHGLPDFGSYLDPSTNCDKKVLKAVLSQLSSMGGEVVPSMSAQMAAAVSSTWNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTP1</accession>
    <name>F2CTP1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123403555</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK354993">
        <property type="protein sequence ID" value="BAJ86212.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953415.1">
        <property type="nucleotide sequence ID" value="XM_045097480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CTP1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617080"/>
    </dbReference>
    <dbReference type="GeneID" id="123403555"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0511780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0511780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0511780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0617080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403555"/>
    <dbReference type="OMA" id="KLCCGEV"/>
    <dbReference type="OrthoDB" id="659547at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CTP1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090575" evidence="7">
        <location>
            <begin position="26"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ86212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="171" mass="16553" checksum="E34F6C67C46A02DB" modified="2011-05-31" version="1">MALAGGATTAACLLVLLAIAHAAGAASHHAPAAAPAPDCTEALIGLADCLDYVLPGSKSAKPSKTCCGEVKTAVGTPATVKCLCEAMAAKETPIPINMTRVLALPGACGEPASVLNKCHALPGGAPAEAPAPSPSSGSAAASPPKANASARSPATILVATVAASLLAFYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2D128</accession>
    <name>F2D128_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123400010</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357585">
        <property type="protein sequence ID" value="BAJ88799.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950341.1">
        <property type="nucleotide sequence ID" value="XM_045094406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D128"/>
    <dbReference type="SMR" id="F2D128"/>
    <dbReference type="PaxDb" id="4513-MLOC_52707.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497810"/>
    </dbReference>
    <dbReference type="GeneID" id="123400010"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0412980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0412980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0412980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497810"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063973_3_0_1"/>
    <dbReference type="OMA" id="WFAIFRE"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D128">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000226">
        <property type="entry name" value="E3 ubiquitin ligase BIG BROTHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033276">
        <property type="entry name" value="BB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400:SF6">
        <property type="entry name" value="OS09G0525400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="125"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ88799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="171" mass="19130" checksum="773A3726EF112AB1" modified="2011-05-31" version="1">MAAPNTYAVHVSSETHKIEAWCESDEALARQLQEEEDSLAIREFAGSVSLEPSSPAVEYRPANNAAQVATEDNVDPDNMSYEQLQAIGEEVGTQSRGLSDDLISYLEPFRNKCNFFSSKKNTDECVICKSNYKSREKLIRLPCSHCYHAGCITRWLKINKACPVCNEEVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2D330</accession>
    <name>F2D330_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123411622</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK358287">
        <property type="protein sequence ID" value="BAJ89501.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960522.1">
        <property type="nucleotide sequence ID" value="XM_045104587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D330"/>
    <dbReference type="SMR" id="F2D330"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681680"/>
    </dbReference>
    <dbReference type="GeneID" id="123411622"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0565950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0565950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0565950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0681680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411622"/>
    <dbReference type="OMA" id="EMPKTLX"/>
    <dbReference type="OrthoDB" id="8093034at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D330">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12619">
        <property type="entry name" value="RRM2_PUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000301">
        <property type="entry name" value="Nucleolysin TIAR-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000191">
        <property type="entry name" value="Oligouridylate-binding protein 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000598">
        <property type="entry name" value="Oligouridylate-binding protein 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF5">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="73"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="158"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="282"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ89501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="447" mass="48761" checksum="A2FE217AC0647B1D" modified="2011-05-31" version="1">MNGAGASQQQQQQQQRLRQQQQQQQQALLMQQALQQQQYQSGVLAAAAAAAMTQMEPVSNGNLPPGFDPSTCRSVYVGNVHPNVTENLLIEVFQGSGPVERCKLIRKEKSSFGFVDYYDRRSAALAIMTLHGRHIYGQAIKVNWAFASTQREDTSGHFHIFVGDLSSEVNDATLYACFSTYPSCSDARVMWDNKTGRSRGYGFVSFRNQQEAETAITEMTGKWLGSRQIRCNWATKTNAEEKQETDNHNAVVLTNGGSSNAAMEANQDTGSKENPENNPDFTTVYVGNLGHEVNRDELHRHFYNLGVGAIEEVRVQQEKGFGFIRYSTHGEAALAIQMANGLVVRGKPIKCSWGNKPTPPGTSSKPLPPPIASYQPVAMAGVPQGFTAAELLAYQRQLALSQAAAGQIAGQHGLAGQVSAGLLAAGSQALYDGYPNQTSAQQLMYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F2D6T1</accession>
    <name>F2D6T1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
            <ecNumber evidence="4">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123444625</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="4"/>
    <dbReference type="EMBL" id="AK359593">
        <property type="protein sequence ID" value="BAJ90802.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977346.1">
        <property type="nucleotide sequence ID" value="XM_045121411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D6T1"/>
    <dbReference type="SMR" id="F2D6T1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0295260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0295260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0295260"/>
    </dbReference>
    <dbReference type="GeneID" id="123444625"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0245730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0245730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0245730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0295260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0295260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0295260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444625"/>
    <dbReference type="OrthoDB" id="3191556at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000045">
        <property type="entry name" value="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000009">
        <property type="entry name" value="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527">
        <property type="entry name" value="4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="156"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ90802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0295260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="406" mass="44279" checksum="48B548C195886E13" modified="2011-05-31" version="1">MACSAHLLSQSLCQPHRSCPAPSKRHLRFQARPAAAASAGVSSSARSRRSSSIRVAASAEQGRKKIELTYDAQAKFNQLADQIDKDVGITRLNLFSPCKINVFLRITGKRPDGFHDLASLFHVVSLGDTIKFSLSPSKSKDRLSTNVAGVPVDESNLIIRALDLYRKKTGTDKHFWIHLDKKVPTGAGLGGGSSNAATALWAANQFSGSIASEKDLQEWSGEIGSDIPFFFSRGAAYCTGRGEIVQDIPNLLPENLPMVLIKPPEACSTAEVYKRLRLDQTSQADPLALLKEITQNGISQDVCVNDLEPPAFEVLQSLKKLKKRFIASNRGDYSAVFMSGSGSTIVGIGSPDPPAFVYDDEDYKDVFVSEARFLTREENEWYREPMSSSATFSKEDSLSEAAPVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7M2</accession>
    <name>F2D7M2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401367</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sugar transporter involved in the transport of CMP-sialic acid from the cytoplasm into the Golgi. May transport important nucleotide sugars such as CMP-Kdo (2-keto-3-deoxy-D-manno-octulosonic acid) in physiological conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nucleotide-sugar transporter family. CMP-Sialate:CMP antiporter (TC 2.A.7.12) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK359884">
        <property type="protein sequence ID" value="BAJ91093.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951089.1">
        <property type="nucleotide sequence ID" value="XM_045095154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D7M2"/>
    <dbReference type="SMR" id="F2D7M2"/>
    <dbReference type="STRING" id="112509.F2D7M2"/>
    <dbReference type="PaxDb" id="4513-MLOC_63767.5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592620"/>
    </dbReference>
    <dbReference type="GeneID" id="123401367"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0491180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0491180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0491180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592620"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="419167at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D7M2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ91093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="404" mass="44842" checksum="36EC7589F4AEE69D" modified="2011-05-31" version="1">MQRNGVMECTACRSRLVVPSPRSVSRAYDKHHNKITSKFRALKFLLVVGDCMLVGLQPILVFMSKVDGKFQFSPISVNFLTEVAKVIFAIVMLIIQSRKQKVGEKPLLARSTLMQAARNNVLLAVPALLYAINNYLKFIMQLYFNPSTVKMLSNLKVLVIAVLLKFIMRRRFSVIQWEALALLLIGISINQLRTAPAGDTAFGLPITAIAYIYTLIFVTVPSLASVYNEYAMKSQDTSIYLQNLFLYGYGAIFNFLGILGTALFQGPENFNILQGHSRATMFLICNNAAQGILSSFFFKYADTILKKYSSTVATIFTGLASAAFLGHTLTINFLLGISVVFISMHQFFSPIAKVKDDKPGELLELQDTQNHRSSDSSFVNMTAGAADDASHQLGTDERQPLLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDU6</accession>
    <name>F2DDU6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123449263</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK362063">
        <property type="protein sequence ID" value="BAJ93267.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK365437">
        <property type="protein sequence ID" value="BAJ96640.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982345.1">
        <property type="nucleotide sequence ID" value="XM_045126410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDU6"/>
    <dbReference type="SMR" id="F2DDU6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449263"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0328710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0328710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0328710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0395140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449263"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF114">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="329"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="399"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ93267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="469" mass="50166" checksum="E1949D82F7773541" modified="2011-05-31" version="1">MAPRLTLLRRLSTHGDPNLAALLAVLRSPEASSTPLPHALSRAFPSPSDAFPLHTLPDLLPRLPSPLLSLRFLLWRLPPSPPLPSPHTLSSLAASLPDLPSAVPLLLSSSPRPLPLPHYALLLGISAHAGLFPVSLAVLRHMRSSGLAPDVACFHSALRAARWHADVSAVLDIMSRSGVDPTVPLIVTAVHRLAYRGDFEGARCLIDKMPEFGCAASVVVYTAMLDGMLAFGQVDAAVGLLEEMEGGRLGAGCVPNVVSYTCLAKCLCRKGRMVEALSVLDRMVARGVMPNRVFVRTLIDGFCAARGAGLLSKAYDVVERLVVDGTLSSGQCYNVLLVGLSVAGMTAEAEGLAQRMMKTGVQLSPLAGSAMARELCQRKMWLHACCWLRLMDENGVLCDSDVYAGVLLGLCQEGHVLEASALVRKVMDRGIRIDASCADCLVQLLKQHGDEELASHVLGLRRSPEVMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2DH41</accession>
    <name>F2DH41_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363208">
        <property type="protein sequence ID" value="BAJ94412.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DH41"/>
    <dbReference type="SMR" id="F2DH41"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520250.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520250.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0520250.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520250.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04714">
        <property type="entry name" value="BAH_BAHCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="26"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="145"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ94412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="227" mass="25695" checksum="4BC80D2FA6CAF7E7" modified="2011-05-31" version="1">MSKTPGKSPRTPRRTLESYTIKGSDGVIRPGDSVLMKAPDSSKPPYVAKIEEIEAAGPRGANVKVKVRWYYRPEESIGGRRPFHGEKEVFLSDHQDVQSADTIECKCNVYSFRDYTKLAAVNPEDYFCRFEYKSITGSFVPDRIAVFCKCEMPYNPDDLMIQCEECSDWFHPACIGKTIKEAKKLENFTCEGCVAENGNANGVKNENSHESTGESDEKQVQSKRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2DHR5</accession>
    <name>F2DHR5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123398204</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant ACBP60 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK363432">
        <property type="protein sequence ID" value="BAJ94636.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948636.1">
        <property type="nucleotide sequence ID" value="XM_045092701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DHR5"/>
    <dbReference type="SMR" id="F2DHR5"/>
    <dbReference type="STRING" id="112509.F2DHR5"/>
    <dbReference type="PaxDb" id="4513-MLOC_55001.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0443870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0443870"/>
    </dbReference>
    <dbReference type="GeneID" id="123398204"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0367600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0367600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0367600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0443870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0443870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0443870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398204"/>
    <dbReference type="eggNOG" id="ENOG502QWE3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015174_1_0_1"/>
    <dbReference type="OrthoDB" id="626385at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DHR5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080142">
        <property type="term" value="P:regulation of salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046829">
        <property type="entry name" value="Calmod_bind_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046830">
        <property type="entry name" value="Calmod_bind_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046831">
        <property type="entry name" value="Calmodulin_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012416">
        <property type="entry name" value="CBP60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713:SF10">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713">
        <property type="entry name" value="OS02G0177800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20452">
        <property type="entry name" value="Calmod_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20451">
        <property type="entry name" value="Calmod_bind_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07887">
        <property type="entry name" value="Calmodulin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Calmodulin binding protein-like N-terminal" evidence="9">
        <location>
            <begin position="168"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Calmodulin binding protein central" evidence="10">
        <location>
            <begin position="326"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Calmodulin binding protein C-terminal" evidence="11">
        <location>
            <begin position="403"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="94"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ94636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="636" mass="71469" checksum="E3E8B32856CA8ADA" modified="2011-05-31" version="1">MAPPKRQLALTGAGAGREEAKRLRVGVANASAAGWQAPTSPASPGTRLMRRTVLVVLFLLRMSDAITVTESISQIGRMVQRKMEGFQKFQALMIRKLENFEEKMEAKLENLEEKLEGKLGKIEEKMESMSDEVKKLARLHSNRHADEHPRLEPFLESTTASEPNTNIRLRFLDGLKTPIYTDKIITSESNAAIRIGVFDGDKMISEGQLSKAKVEILVLRGDFCSNGRESWTEEEFNSHIAQGRNGQGSVLGGDCSAWLNNGKASLGKILFREGSSRTPNRKFIVGGRVCMNKKVGGVRVQEAVMEPVAVLDRRNEVNEKRHPPRLDDEVYRLEEISKNGTYHGRLMNAQIFKVEDFLKALNKGADELREMVLQIKKRGNAWERMVKHARECYLADRPELKACCSVEGNVVIFLNCVHDLVGAIFTGVYISRDNFDPAKKAQAYELKERVRDQLDNLPFDYVMNGNLPEKVPSSTYSSLDAVILGPDAAHQPNENQLHDRINNVTEPSHQNEYIHGDHNTVNTHHDQDESIPPLGEQQPTTSMTHLQWDDESLDWPFEVQTNIHSQVQVPISMDGGNAMEASTSVQHNVLPQRFVPTHVQESIPRIHYSENPANHAAGSSTWQDDIIIPELDYGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJ31</accession>
    <name>F2DJ31_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="10">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123451949</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="8">Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-asparagine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="10"/>
    <dbReference type="EMBL" id="AK363899">
        <property type="protein sequence ID" value="BAJ95102.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK375085">
        <property type="protein sequence ID" value="BAK06280.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984455.1">
        <property type="nucleotide sequence ID" value="XM_045128520.1"/>
    </dbReference>
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    <dbReference type="SMR" id="F2DJ31"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092430.1"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0092430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092430"/>
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    <dbReference type="KEGG" id="hvg:123451949"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
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        <property type="organism ID" value="2687"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:response to stress"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Asn_synthase_B_C"/>
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        <property type="entry name" value="AsnB"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK09431.1"/>
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            <begin position="2"/>
            <end position="185"/>
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        <location>
            <position position="98"/>
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        <ligand>
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        </ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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            <position position="267"/>
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        <ligand>
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        <ligand>
            <name>ATP</name>
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    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022962"/>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
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            <taxon>Triticodae</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
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        <property type="molecule type" value="mRNA"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174650.1"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426978"/>
    <dbReference type="eggNOG" id="ENOG502QTZN">
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        <property type="component" value="Chromosome 2H"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="WIP1/2/3"/>
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    <dbReference type="PANTHER" id="PTHR34562:SF7">
        <property type="entry name" value="OS04G0471300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34562">
        <property type="entry name" value="WPP DOMAIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="266"/>
            <end position="285"/>
        </location>
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        <location>
            <begin position="1"/>
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            <begin position="39"/>
            <end position="58"/>
        </location>
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        <location>
            <begin position="118"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK00431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="298" mass="34008" checksum="FB32F5431C3FA0DA" modified="2011-05-31" version="1">MSMSYDGSQSEDRQSEEVQSAYKKFSAGDEDVIRGFVNLDENEEQNEENEWSWVPQEEDPLARSASSLEKAQEVLEKFFKKNDVLESEMLKLSELGKEFVAEDSHDGNIGFPDIEADVLGMNQKMECLELKLKEASDTITEKDSRLSELQSLINSAHTPTLQTETVNTDELETELESHLQDKIDAEIQCLVMMKARQNWQARAEDQIALEEHRVSAGDDTRMLLKLQDTESKIVMLKEQVDRLEAHEKELSVTTEILRMQSKTFKIGLFGLLQLIMLCLSLKMFFAQDSARFGDLVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZC8</accession>
    <name>F2DZC8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cohesin subunit SA-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">SCC3 homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Stromal antigen 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Stromalin-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123448371</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the meiosis-specific cohesin complex, which also contains the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer. Such complex likely contains RAD21, or the meiosis-specific related protein REC8. Interacts with CCDC79/TERB1; recruiting cohesin to telomeres to develop structural rigidity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369248">
        <property type="protein sequence ID" value="BAK00450.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981151.1">
        <property type="nucleotide sequence ID" value="XM_045125216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DZC8"/>
    <dbReference type="SMR" id="F2DZC8"/>
    <dbReference type="STRING" id="112509.F2DZC8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361590"/>
    </dbReference>
    <dbReference type="GeneID" id="123448371"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0301340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0301340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0301340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0361590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0361590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0361590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448371"/>
    <dbReference type="eggNOG" id="KOG2011">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DZC8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000449">
        <property type="entry name" value="Cohesin subunit SA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="16">
        <location>
            <begin position="276"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1022"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1022"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1101"/>
            <end position="1121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAK00450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1121" mass="127593" checksum="D0114BDDF2EDFE31" modified="2011-05-31" version="1">MAETLASTRRPKRGRPPRPRESDFVTGEEFEDEEEGEDHAEAADGLAPPRSKRKREASAAAAAALEDLTLIDIVKHNGRLISHAAKRLVEDYESNPKSVLFQILTMLFEACGARHDIYASDLHEAAVDDIVFKLAELARKGLVDDNYSSKRKDLKNFKENLVTFWDSLVLECQNGPLFDDNLFTTIKDYVVAISCTPPRVYRQVASLVGLQLVTSFISVAKTLSGQRETTQRQLNAEKKKKSDGPAVESLNKRLSITHENITYLEESMRKIFSGLFMHRYRDVDPEIRMLCIKSLGIWVVSYPSLFLQDIYLKYLGWTLNDKNAGVRRTSILALQSLYDVDDNIPSLGLFTERFYSRMIQLADDIDISVAVPAIGLIKQLLRHQLLSDDDLGPLYDLLIDEPPMIRRAIGELVYDHLIAQNCKTPSVARDGDNESSEIHISRMLHILREFSDDPVLSSYVIDDIWDDMKAMKDWKCIISMLLDETPIAELTDMDGTNLVRMLRASAKKAVGERIVPATDIRKMYYNKSQKEILENSKSDITNALMKRYPQLLRKYLPDKAKISPLIDMMMLLKLEMYSLKRQEQNFKAAIDLIVDAFFKHGDKDTLRSCIKVIASCCMKCQADLLDYAENKLKTLEDELVLKVKTAIKEVEAGDDEYSLLVNLKRLHELQLSKPVKNDGLFEDMYRILSHLREMDNEVKSFLLINMFLEVAWCLHAIDVENPSETSIDGLQSKQKALFEQLYYFLVVLSNYQKEGRSTTVLSCRVCIITAEMWCLFKKSKYSSTKLKNLGYLPQLDVVQKFWKLCEQQLNISDDTEDEDANEEYIEDTNRDAVMIAAAKLLLADTVSKDYLGPEIVSHYVSHGASTTEIIKHLITALKKNANSDIAALFFEALRRAYERYMTYLRDGENQNLIAKSYSECQDLANRLAGYYVGTVRIKNKSEILKIIQCGVQFAFVDLPKQLSFLEAALMPFVSKLPSSDIPDILTDVQKRAQDTNMNEDPSVWRAYLTFVEHLREKHARNEVFHEEKEEKPVKRRGRPRKPRDEPVRNLFDGNKSSDEESVSGSDQRGHGGDDDDEDDAFDQPLINTFRPSASKLRSLKGVSQQGTSSQRNAPTASGSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2E5M1</accession>
    <name>F2E5M1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Peroxidase</fullName>
            <ecNumber evidence="21">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC123417720</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="18 21">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="18 21">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="21"/>
    <dbReference type="EMBL" id="AK371445">
        <property type="protein sequence ID" value="BAK02643.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962855.1">
        <property type="nucleotide sequence ID" value="XM_045106920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E5M1"/>
    <dbReference type="SMR" id="F2E5M1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0081950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0081950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0081950"/>
    </dbReference>
    <dbReference type="GeneID" id="123417720"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0067160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0067160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0067160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0081950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0081950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0081950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123417720"/>
    <dbReference type="OrthoDB" id="604609at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 18 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 21" id="KW-0349">Heme</keyword>
    <keyword evidence="15 21" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 18 21" id="KW-0408">Iron</keyword>
    <keyword evidence="7 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 21" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044524099" description="Peroxidase" evidence="21">
        <location>
            <begin position="24"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="22">
        <location>
            <begin position="26"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="121"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="200"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BAK02643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0081950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="326" mass="34890" checksum="C54C511116C92694" modified="2011-05-31" version="1">MAMAIRSLLLPLALLALAASSAAVANLEIGFYSKTCPDAEKIVREEMAKIIAAAPSLAGPLLRLHFHDCFVRGCDASVLLESTEGNVAEKDAKPNKSLRGFGSVERVKAKLEAACPGIVSCADVLALMSRDAVVLAKGPFWPVALGRRDGRVSSATEASNELPPASGDVPLLAKIFASKGLGLKDLVVLSGAHTLGTAHCPSFADRLYNTTSGSVDPSLDSEYADKLRLKCRSVDDRTMLSEMDPGSFKTFDTSYYRHVAKRRGLFRSDAALLFDATTRDYVQRIATGKLDGDFFSDFSASMIKMGDVGVLTGTQGEIRKKCYALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F2E645</accession>
    <name>F2E645_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123427007</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371619">
        <property type="protein sequence ID" value="BAK02817.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966871.1">
        <property type="nucleotide sequence ID" value="XM_045110936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E645"/>
    <dbReference type="SMR" id="F2E645"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0175580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0175580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0175580"/>
    </dbReference>
    <dbReference type="GeneID" id="123427007"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0145130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0145130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0145130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0175580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0175580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0175580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427007"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19145">
        <property type="entry name" value="AKR_AKR13D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000048">
        <property type="entry name" value="Probable aldo-keto reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF37">
        <property type="entry name" value="ALDO-KETO REDUCTASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK02817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0175580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="344" mass="37730" checksum="C68FAD1AA0BEF970" modified="2011-05-31" version="1">MAAAPVTVPRMKLGSQGLEISAQGLGCMGMSAAYGERKPEQDMVALLRHAVAAGVTFLDTSDIYGPHTNELLLGKALQGGVREKVQLATKFGITVTREISGNPAYVRAACEASLARLGVDCIDLYYQHRIDKNVPVEITMGEIKKLVQEGKVKYVGLSEASASTIRRAHAVHPITAVQLEWSLWSRDVEEDIIPTCRELGIGIVAYSPLGRGFLSTGPKLMDTVRDDDFRKNLPRFQPENLKKNAAIFERVSEMAARKGCTSSQLALAWVHHRGTDVCPIPGTTKVENLNQNVRAMSVQLMVEEMAELESYAAMDAVQGDRYHSTFLNTWKDSETPPLSSWKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F2E677</accession>
    <name>F2E677_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123442619</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="AK371651">
        <property type="protein sequence ID" value="BAK02849.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974643.1">
        <property type="nucleotide sequence ID" value="XM_045118708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E677"/>
    <dbReference type="SMR" id="F2E677"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228060"/>
    </dbReference>
    <dbReference type="GeneID" id="123442619"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0189160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0189160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0189160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0228060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442619"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000042">
        <property type="entry name" value="Glutathione S-transferase F9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF95">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="96"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="550"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="638"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="673"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="793"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK02849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="826" mass="90440" checksum="19DEC64CA469E0C1" modified="2011-05-31" version="1">MAGLGTGAGAPLVKVYHEKSMILPDVSRVLACLYEKNIEFETVKDSYKDILRLQSMRSVPVPFYDGPTFRQESRAICRYIAETYEQRGYPFLLGKDVLERASIEQWLRHEEHAFDPPSRALFCHLAFPFPDEDTSDIDREKRKLEEVLEVYEQRLGESEFLAGNKFTLADLVHLPNTHHIVTSDEFAYLYDSRKNVQRWWNAISGRDSWQQVVRDMQDVEEQYQMEELEQQQQLEQQWQWQPEGPEPPAAYGGRTIRIDPRKQAGMESRTILVPPPGSGTISSSSFTVQPLPAETTSHGPNSPGKMKESNFFPTTEKTPAKSKQRNPTTQKKPAASSVETTTSNFFTPAAPPSTTKMSQRTDADKPTSKDASSPTRPSQGSSKKAPDKLHLSDDEAATLSKPMLSQEARKPTDKTPYSQQPSEQAKKAAADQKDAAHLPKHVAFRDIQNETAPQARSRGAKDSTKEGREADQKRAATALTRELPPGSQNTPQAPSADPKPSDSAPMKEGANNEDDRFSTKRLRKMLEESDPAVLKPQSTDMQPPPVQADGARDPRTPVTADERRATSPPKGGVAGDDRGASPPRKSPSANERQSSPQMPSQSPPGESATPPAPLQAPASDAPGENEAVKPSIADPRGISTTPKRLASTPGTQRQTATATDQLSDQAPAASSFADRRNEKTGIDEARQTATVAPDVQPGGRAPNSSGSKPISSKDISKQTDETLYYRSSETSREMPSSAPEKSMRQQQLQSDKSITSPQGIGKQGSEAAPPVSGTEKPGRTQGQDLVESREQTSPDTQQVKNNRNNSKSDGSSKPTQFDGNKGDVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F2EA70</accession>
    <name>F2EA70_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123451198</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
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            <authorList>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
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                <person name="Hori K."/>
                <person name="Itoh T."/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GTD-binding" evidence="7">
        <location>
            <begin position="62"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK04387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="479" mass="51457" checksum="B96DC506A402EC5B" modified="2011-05-31" version="1">MHAMADAGGPPPNLCPLCGHPTGSTTSPSRPSSPPLAATRPTLKRNSPPEAPPAVVRVEIGDEAAALREALARQQAALADLQAELDAERGAAAGAASEAMTMILRLQREKAEAMMEARQFRRYAEEKMSHDAAELAALEETIAKRDASVRGLQSLSRLSVANAASLHSSPRVSSTPRHPSTPSTVGAGGYYPPLRCFSGRFNDHPPTASEADVLDMQTPRDHLARLSQRVQMLEHRAPPTTTTTPIIRVAPGSAFPRNPRPFSDTDSLDFGDGEYFPDDDCGASDRVYTVDAIHGRGVPLVVPEGSICGGTPAGSECCAGGPWAEDEEMRRLSARLQALEADRETMRQAIISMGAEKAQVVLLKEIAQQLCKDAPPALPAVTVGHHYYKGAAPPAVTVTVPRPQRPMVMHRMVLKSQPTRTSFFATVVKWVASIVLWRRKSSRIKYPIGQCGNNVGLMLLLDKSSKSAGNGHHKPPKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>M0UEX6</accession>
    <name>M0UEX6_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123445633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978581.1">
        <property type="nucleotide sequence ID" value="XM_045122646.1"/>
    </dbReference>
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    <dbReference type="PaxDb" id="4513-MLOC_10316.5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0326130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0326130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0326130"/>
    </dbReference>
    <dbReference type="GeneID" id="123445633"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0272040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0272040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0326130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0326130.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF30">
        <property type="entry name" value="SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0326130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="246" mass="28084" checksum="A0131CAAC4B0576F" modified="2013-04-03" version="1">MDIQNLISCCPVLTDLRLVRFGGIRCLNIQAPKLEYLKVHGDFEDIALDAPNLEVATLSIDYRSNIYQSVPIAHDKESYVKKALGSLSEIKSLSITVFFLKYLSKGCILMKLPAVFRRLEYISLMINFEDQRQVLTACSLLQNAPNLKELRISSYRWAIWYPDEASIQELTMQTQTQMGHLVTASMYCFEGLDCEIDFVAKLLSWAPALEEVKIEWLGERERSKVVAKLLLDLPRVSPRAKLTVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>M0VE77</accession>
    <name>M0VE77_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0VE77"/>
    <dbReference type="PaxDb" id="4513-MLOC_24517.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321090.1.CDS1"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321090"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0VE77"/>
    <dbReference type="OMA" id="SKGPACH"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34998:SF10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34998">
        <property type="entry name" value="OS04G0357400 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="110" mass="11715" checksum="43C2EE09F1486014" modified="2013-04-03" version="1">MDRKVCISLVILALMLAGSDMTAFAGNTASIDVAVAVSFRQPMPTPSSWSPVRLADGVAVEEAHRRVLGGFYTPALNPKGPDCLGPCLKPGQPYTDHGCKSIYLCRNKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>M0VGW1</accession>
    <name>M0VGW1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0VGW1"/>
    <dbReference type="SMR" id="M0VGW1"/>
    <dbReference type="STRING" id="112509.M0VGW1"/>
    <dbReference type="PaxDb" id="4513-MLOC_27819.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0694750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0694750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0694750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0576760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0576760.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0576760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0694750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0694750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0694750"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0VGW1"/>
    <dbReference type="OMA" id="MCIAGET"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF2">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0694750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="168" mass="18870" checksum="02FFDD48276EFC40" modified="2013-04-03" version="1">MLYGCTSRMSCDLLLVYEFIANGTVADHVHGSRSVERGLTWPLRLNIAIETAEALAYLHAVEIIHRDVKTTNILLKNIFHVKVADFGLSRLFPLEVTHVLTGPQGTPGYVDRVYHKCYKLTDKSHVYSFGVVLVELISSKPAVDMNRSHSEINLANMALNRIQNHEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>M0VMI6</accession>
    <name>M0VMI6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0VMI6"/>
    <dbReference type="SMR" id="M0VMI6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VMI6">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22778:SF55">
        <property type="entry name" value="ESTERASE C25G4.2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22778">
        <property type="entry name" value="OVARIAN CANCER GENE-2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="139" mass="15172" checksum="648A680EFA139716" modified="2013-04-03" version="1">MVKNGPFDGLLGFSQGATLSALLIGYQAQGKVLNDHPPIKFMVSVSGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLKVPSEELASAFADPLIIRHPQGHTVPRLGLHSSLSLSYANNCRIVFAFHSVTLHRIALSCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>M0WE43</accession>
    <name>M0WE43_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NBS-LRR resistance-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0WE43"/>
    <dbReference type="SMR" id="M0WE43"/>
    <dbReference type="PaxDb" id="4513-MLOC_50140.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0021130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0021130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0021130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0016600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0016600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0016600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0021130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0021130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0021130"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WE43">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34630:SF100">
        <property type="entry name" value="OS02G0118900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34630">
        <property type="entry name" value="OS11G0677101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0021130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="63181" checksum="4DC5CFB8048CCF5B" modified="2013-04-03" version="1">MAKWVGNGKCHLFSLLEEITIIGCLQLTELPFSHPAHDQAKQEENMTWFPKLMKLNITGCPKLASLPPIPWTQAPCSAEIRRAGSVFEKLVYSKNYKSELSLEVEGKDGQHGLWNGLAFHNLAGLKELEVNNCPPLPLIHLQKLKSLKSLTVFDMSNLLLLFECESYNTECPLPVEQIKIWGCGADGKEMTQLLSHFPKLTELVLTCCEKITELGVVELQTEMTTPSSSGNEIEIEHAQAGHHQTRAEEVEEAVAGGEGLLLLPRQLQELSIYHCSELRLLSDSLGKDNGNPRGGGLQSLSSLQSLFIFDCPRFLSSYPSSASSCFPFPTSLRWLSLSRVESMETLTPLSNLSSLTSLTVYNCGDLRGEGLWPLVARGRLTELGIFGTRKFFTGSEPSRLHGQQIPSSSSKFEHLTTDDLTGVLTAPICKLLSSSLTELSFWENKEVERFTEEHEEALHLLNSLQELVFWESEKLQRLPAGLTQLASLKILRISECPAIRSLPKDGLPSSLQELEIKDCPAIKSLPKDGLPSSLRKLEVCNGISEELKRQCRKLKGTIPVIKDYHDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y406</accession>
    <name>M0Y406_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123444512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044977180.1">
        <property type="nucleotide sequence ID" value="XM_045121245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y406"/>
    <dbReference type="PaxDb" id="4513-MLOC_65509.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0291710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0291710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0291710"/>
    </dbReference>
    <dbReference type="GeneID" id="123444512"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0242540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0242540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0242540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0291710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0291710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0291710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444512"/>
    <dbReference type="eggNOG" id="ENOG502QQ60">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y406">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF28">
        <property type="entry name" value="OS01G0769000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0291710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="813" mass="87506" checksum="A33D199CD3D19231" modified="2013-04-03" version="1">MPGAEADAGGGGGGGGGASSSSAADSFDAGQYAFFGKEPRDGLELGCLEVDGGHGNGGGFSGAENGLYRLSSVGEEIDNLSNLSDVDDLASTFAKLNRTVSGARNPGVIGDRRSISRGSSLTVDWTEDVEFPNYQDILENEELQEGKRWWSSQSHSLVQQGDNKPLSRTSSYPQQPLQHRSSEPITGPKSPPFTSFPPPGGRSPHPAQGLTRHGSIPSFGAGIQMGSPSMPLPGSPYHMVGLPHGLPYGGSMPFGGPNMHVSNPMQNDWSNQANLFTGEHLNLLPNLLQKQISLPNSPMSSLLFSQQQQRLAQLQPPHQNYLNLPPHLFYHHHPPEIPGRFESATSLPSSRDKRSRSGRGKHSLRFSQPPSDTTGSQNGEGGGLKFRSKYMSSEEIESILRMQHSASHSSDPYVVDYYHQACMAKKGSTSRQKISFSPTSIKDLPSKSRSNGDQHAYLQVDALGRVSFSSIRRPRSLLEVDHPSSGEGPHDQKSSVRSLEKEPMLAARVTVEDAIGLLLEVDDIDRLLQSSQAQDNSFQLRRRRQVLLEGLAASLQLVDPLGPSKSTNSSGLSGLAPKDDIVFLRIVSLPKGRKLLARYLRLIAAGSELTRIVCMAIFRHLRFLFGGLPPDSSAAETTVALAKTVSTCVHHMELGALSACLAAVVCSSEQPPLRPLGSSAGDGASLIIKSVLDRATELLTDQHAVSSYTSSNRALWQASFDAFFGLLTKYCLSKYESILQMFRMQASGSVIGSEASQATSREMPVELLRASLPHTNEQQRQMLLDFAQRTTPASSFNPPGASGGHITSESVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>M0YQT0</accession>
    <name>M0YQT0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123409059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044957983.1">
        <property type="nucleotide sequence ID" value="XM_045102048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YQT0"/>
    <dbReference type="SMR" id="M0YQT0"/>
    <dbReference type="FunCoup" id="M0YQT0">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0YQT0"/>
    <dbReference type="PaxDb" id="4513-MLOC_71862.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0745710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0745710"/>
    </dbReference>
    <dbReference type="GeneID" id="123409059"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0618510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0618510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0618510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0745710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0745710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0745710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409059"/>
    <dbReference type="eggNOG" id="ENOG502QRNX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YQT0"/>
    <dbReference type="OMA" id="QNASNMD"/>
    <dbReference type="OrthoDB" id="440455at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YQT0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245">
        <property type="entry name" value="UBIQUITIN SYSTEM COMPONENT CUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245:SF1">
        <property type="entry name" value="UBIQUITIN SYSTEM COMPONENT CUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="278" mass="30479" checksum="8690D4D5D4DE2B0B" modified="2013-04-03" version="1">MSAVVCGKRSSSIFADDLLLQQASSSPPSHHSPAAKRSRYAHHHHRRDALLGQLRAAFPAMDPQLLERALEASGDDLDDAIKSLKELHLMESNQANPSATGSTFENGPTAVQPSVEGIVASSGVDTATQHQPAADSHQPSNSGPEWVDLFVREMSNASDMDDARARASRALEALTKSILEGAGAEAAQSLHQENMMLKEQMTAVLSQNAVLKRAVAIQHERQKEFDERSHEVQGLKQLVLQYQEQLRTLEINNYALQMHLKQAQQSSSMPGRYNPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-07-05" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q6RYF1</accession>
    <name>Q6RYF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell number regulator 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">615K1.4</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Mol. Genet. Genomics" volume="268" first="488" last="499">
            <title>Cereal genes similar to Snf2 define a new subfamily that includes human and mouse genes.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Echenique V."/>
                <person name="Busso C."/>
                <person name="SanMiguel P."/>
                <person name="Ramakrishna W."/>
                <person name="Bennetzen J.L."/>
                <person name="Harrington S."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="12471446"/>
            <dbReference type="DOI" id="10.1007/s00438-002-0765-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2004" name="Science" volume="303" first="1640" last="1644">
            <title>The wheat VRN2 gene is a flowering repressor down-regulated by vernalization.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Loukoianov A."/>
                <person name="Blechl A."/>
                <person name="Tranquilli G."/>
                <person name="Ramakrishna W."/>
                <person name="SanMiguel P."/>
                <person name="Bennetzen J.L."/>
                <person name="Echenique V."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="15016992"/>
            <dbReference type="DOI" id="10.1126/science.1094305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AY485643">
        <property type="protein sequence ID" value="AAS58477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6RYF1"/>
    <dbReference type="FunCoup" id="Q6RYF1">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="112509.Q6RYF1"/>
    <dbReference type="PaxDb" id="4513-MLOC_38501.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0346950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0346950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0346950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q6RYF1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAS58477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27936" checksum="5F7387E6F3345B31" modified="2004-07-05" version="1">MAEEGHPSRYVKLTKDQDAPSEDIRPGELNQPVHVPQLEGRRCGECGQVLPESYEPPADEPWTTGIFGCTDDPESCRTGLFCPCVLFGRNVEALREDIPWTTPCVCHAIFVEGGITLAILTAIFHGVDPRSSFLIGEGLVFSWWLCGTYTGIFRQELQKRYHLKNSPCDPCMAHCCLHWCANCQEHRERRGRLADHSAVPMTVVNPPPVQEMSMSENRDAPAPANPENGSSNKAELEARKSNHDDVEVIPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-21" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q76LM0</accession>
    <name>MATK_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Maturase K</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Intron maturase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">matK</name>
    </gene>
    <organism>
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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        <citation type="journal article" date="2002" name="Genome" volume="45" first="1157" last="1166">
            <title>Molecular phylogeny of the genus Hordeum using three chloroplast DNA sequences.</title>
            <authorList>
                <person name="Nishikawa T."/>
                <person name="Salomon B."/>
                <person name="Komatsuda T."/>
                <person name="von Bothmer R."/>
                <person name="Kadowaki K."/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0150">Chloroplast</keyword>
    <keyword id="KW-0507">mRNA processing</keyword>
    <keyword id="KW-0934">Plastid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000143426" description="Maturase K">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01390"/>
        </source>
    </evidence>
    <sequence length="511" mass="61327" checksum="5A812CEC4743190B" modified="2004-07-05" version="1">MEKFEGYSEKQKSRQQYFVYPLLFQEYIYAFAHDYGLNGSEPVEIVSWNNKKFSSLLVKRLIIRMYQQNFLDNSVNHPNQDRLLDYKIFFYSEFYSQILSEGFAIVVEIPFSLRELSCPKEKEIPKFQNLRSIHSIFPFLEDKFLHLDYLSHIEIPYPIHLEILVQLLQYRIQDVPSLHLLRFFLNYYSNWNSFITSMKSILFFQKENKRLVKFLYNSYVSEYEFFLLFLRKQSSCLPLAYSGTFLERIHFSRKMEHFGIMYPGFSRKTLWFFMDPLIHYVRYQGKAILASKGSFFLKKKWKCYLINFWQYYFFFWTQPRRIHINQLANSCFDFMGYLSSVPKSPLLVRNQMLENSFLIDTRMKKFDTIVPATLLIGYLSKAQFCTGSGHPISKPIWTDLSDWDILDRFGRICRNLFHYHSGSSKKRTLYRLKYILRLSCARTLARKHKSTVRTFMQRLGSAFLEEFFTEEEQVFSLMFTKTTLFSFSGSHTERIWYLDIIGINDLVNPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GZJ0</accession>
    <name>A0A287GZJ0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287GZJ0"/>
    <dbReference type="SMR" id="A0A287GZJ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0083410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0083410.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0101120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101120"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
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        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="288" mass="31448" checksum="3DC484D80581C82C" modified="2017-11-22" version="1">MASLATPSAASTALVTEMMAKGRKSAAALEALLPLQDHAGIRELTADILRCCDRALAALHDTGRKKRKSSPDGSAATQTTRPKRRTRASRGETAAATTRVERKRNWDDGFLWTKYGQKDIRGSGHPRHYFRCAYKLDAGGCPARRQVQRSEEEEEDDPYLYVITYFGDHTCCHRGAEANATLDHVKTHYQSLVLGFGSAGGRSPRPDGDAWSGKTSRSAELAAEVAKVESTPSSDLWPSGPTEGLRSSANDVRCSSSPTSAAWDPSATADWDYFGNCSFDYLSEFFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287JYZ4</accession>
    <name>A0A287JYZ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bowman-Birk serine protease inhibitors family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123439202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Bowman-Birk serine protease inhibitor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971877.1">
        <property type="nucleotide sequence ID" value="XM_045115942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287JYZ4"/>
    <dbReference type="SMR" id="A0A287JYZ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222530"/>
    </dbReference>
    <dbReference type="GeneID" id="123439202"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0184990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0184990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0184990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0222530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439202"/>
    <dbReference type="InParanoid" id="A0A287JYZ4"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287JYZ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00023">
        <property type="entry name" value="BBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.69.10">
        <property type="entry name" value="Cysteine Protease (Bromelain) Inhibitor, subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035995">
        <property type="entry name" value="Bowman-Birk_prot_inh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000877">
        <property type="entry name" value="Prot_inh_BBI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33479">
        <property type="entry name" value="BOWMAN-BIRK TYPE BRAN TRYPSIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33479:SF22">
        <property type="entry name" value="BOWMAN-BIRK TYPE BRAN TRYPSIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00228">
        <property type="entry name" value="Bowman-Birk_leg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00269">
        <property type="entry name" value="BowB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57247">
        <property type="entry name" value="Bowman-Birk inhibitor, BBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00281">
        <property type="entry name" value="BOWMAN_BIRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Bowman-Birk serine protease inhibitors family" evidence="6">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="15444" checksum="EEEE5805B967129B" modified="2017-11-22" version="1">MKMMKRCVVPSMLLMLSLESALLVAAGRPSTATILLPSQGKGQAGMLPVVAPKKKGGEGERPWKCCDFGVCYRALPRACRCLDEVEQCAPTCKSCQPAPSDPSRHVCNDRYTGDIGPTCTETVAAAILNYGAQGTGQDAQVIVAGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W6X3</accession>
    <name>A0A8I6W6X3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123443367</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975649.1">
        <property type="nucleotide sequence ID" value="XM_045119714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W6X3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067420"/>
    </dbReference>
    <dbReference type="GeneID" id="123443367"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0053950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0053950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0053950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0067420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0067420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0067420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443367"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000063">
        <property type="entry name" value="cyclic nucleotide-gated ion channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF101">
        <property type="entry name" value="OS05G0502000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="243"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="490"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0067420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="686" mass="76769" checksum="0D4C496EC70CDAF5" modified="2022-05-25" version="1">MSGELSARSSPSFAASPSGADDARAGESTPLRAEAEGSRPGSAGVKLRRRWQRRLGRWRVGDTWALDPRARWVRDWNRAYLLACAAGLMVDPLFLYAVSVSGPLMCVFLDGWLAAAVTALRCMVDAMHAWNLLTQLRVARAAAAAVARGRRGVADEEQAEADAAAARSLPAYARSRRGMALDFFVILPVMQVVVWVAAPAMIRAGSTTAVMTVLLVAFLLEYLPKIYHSVSFLRRTQDQSGHIFGTIWWGIVLNLMAYFVAAHAVGACWYLLGVQRATKCLKEQCLLTGPPGCASGPVACAAPLYYGGAGAAASVVGDRLAWATDDKARSVCLVSGDKYQFGAYKWTVMLVANTSRLEKMLLPIFWGLMTLSTFGNLESTTEWLEIVFNIITITGGLVLVTMLIGNIKVFLNATTSKKQAMHTRLRSLEWWMKRKDLPQSYRHRVRQFERQRWAATRGVDECQIVRDLPEALRRDIKYHLCLDLVRQVPLFHHMDDLVLENMCDRVRSLIYPKGETIVREGDPVQRMVFIVRGHLQCSQELRNGGTSCCMLGPGNFTGDELLPWCLRRPFAERLPASSSTLVTLESTEVFGLEAADVKYVTQHFRYTFTNERVRRSARYYSPGWRTWAAVAVQLAWRRYKHRKTLSSLSFIRPRRPLSRCSSLGEEKLRLYTAILASPKPNQDDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W8G9</accession>
    <name>A0A8I6W8G9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123420348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044963266.1">
        <property type="nucleotide sequence ID" value="XM_045107331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W8G9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091020"/>
    </dbReference>
    <dbReference type="GeneID" id="123420348"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0074930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0074930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0074930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0091020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123420348"/>
    <dbReference type="OrthoDB" id="1892100at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053350">
        <property type="entry name" value="CV_Inducer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37210">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37210:SF2">
        <property type="entry name" value="PROTEIN CHLOROPLAST VESICULATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="148" mass="15223" checksum="812F322EF41E82E2" modified="2022-05-25" version="1">MPTISCCHQLKPPSPTLHLQPAGGAQAPAHLPALRRACFAAAACVVAVGAMGAVDGAAMARGPVDDGAAHGAALVHVQAPARWSDRRQCPPWHANSLENVVPENLPRPSARRRYNGVAAADKGRAPAPDAVLPFLVLRSGSGMGCFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJF3</accession>
    <name>A0A8I6WJF3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092960.1">
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0092960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0092960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0092960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0092960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="185" mass="21256" checksum="1481D4B1C3DA91C5" modified="2022-05-25" version="1">MAALRHAARRLCERALQQRGALQQRGAQARSSFVADEPRRLLLNGTSPGRRLMSNSSIGGKPASRAEAKGELELLEWQFKKKKEELFDLLIAVKCSDLYPQLSWKDIETHRLISHLASQVEPRPHDPIWRMFRRRRRLNDFNTICAFLGYYGLLAYWVQTNIREYGAASKNSEANSVSEPKNDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJV8</accession>
    <name>A0A8I6WJV8_HORVV</name>
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        <recommendedName>
            <fullName evidence="21">Protein CHROMATIN REMODELING 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123425981</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045109758.1"/>
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    <dbReference type="SMR" id="A0A8I6WJV8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0143700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0143700.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123425981"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0143700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0143700"/>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 1-like isoform X1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
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    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
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    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
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    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45623:SF28">
        <property type="entry name" value="PROTEIN CHROMATIN REMODELING 4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00385">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF06465">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SNF2-rel_dom"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="75"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="514"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="689"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="996"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="16">
        <location>
            <begin position="1704"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
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            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="132"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="227"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="426"/>
            <end position="449"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1413"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1496"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2008"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2071"/>
            <end position="2100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2142"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2188"/>
            <end position="2253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1423"/>
            <end position="1433"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2077"/>
            <end position="2090"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2196"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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            <end position="2253"/>
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        <source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0143700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2253" mass="250800" checksum="34AF01AF794A8828" modified="2022-05-25" version="1">MKERSSLCDSAADGNWVSKYKRKRSKLTASSSNEHEATSPTSGSLMNNGSIKKRSKHDTSISTSAKKIRGHDGYFYECVECDLGGNLLCCDSCPRTYHLECLNPPLKRAPPGNWQCPRCCTKQVSLKLLGNADADTSKRERTRRMRTRTISDSPSPHNKLSLNTRSPIQEKGESNEQVKLSCPGALKEGDPSVKDNEVQKKKPLIVHLKRRSTKELSAITKPLKSGFVGQSSEEKQEKHGVALKMKKHLRSMDLSPKKYKTKRQNSHKDPKRSEAKRLRSLATDVDSDSSMGPSTSPEHRESPPKRRSLDGRTPTFSAKKGKKKVNFVDKQHSEVLAVAGDTIITPQEDLQVDRILGCRLQTSQIISQPHASSEQIELTNVQLEDGAPSSSSSVPTQLLRNDTDTTLEDVCADESAHDACEDHLDGGEMQKENNGKYHEKESVGPEEATKTVSDCCTDHIITIKDSGVVGENIPSVNGELEAVSDVPAAGTSEKGDIKLPLSEVDATVHTKQELTPESKVYGNINEIAGNGHDDTAYEFLVKWAGKSNIHNSWISESELRTLAKRKLENYKAKYGMGLINICKDQWCQPQRVIALRVSLDEVEEALIKWYGLPYDECTWERLDEPIMLKYVHLVTQFKNFECQALDRDVRGNCANARNRQELTVLIQQPKELKGGMLFPHQLEALNWLRKCWFKSKNVILADEMGLGKTVSACAFLSSLCCEFKINLPCLVLVPLSTMPNWMAEFASWAPHLNVVEYHGSARARSAIRQYEWHEGDATQIGKSKKSHKFNVLLTTYEMVLVDATYLRSVPWEVLIVDEGHRLKNSSSKLFSLLNTFSFQHRVLLTGTPLQNNIGEMYNLLNFLQPASFPSLASFEEKFNELATAEKVEELKKLVAPHMLRRLKKDAMKNIPPKTERMVPVELTSIQAEYYRAMLTKNYQVLRNTGKGGAHQSLLNIVMQLRKVCNHPYLIPGTEPESGSPEFLHEMRIKASAKLALLHSMLKVLHSDGHRVLIFSQMTKLLDILEDYLTLEFGPKAFERVDGSVSVAERQAAITRFNQDKTRFVFLLSTRSCGLGINLATADTVIIYDSDFNPHADIQAMNRAHRIGQSNRLLVYRLVVRASVEERILQLAKKKLMLDQLFVNKSESQKEVEDIIRWGTEELFRNNDEVNGKDNDEASDAKHDVSDIEVKHRRKTGGLGDVYEDKCIGSSTKLIWDENAIMRLLDRSSLPTTVAESTDGDLDNDMLGTVKSIDWNDDINDDPGAAEDIPNIDNDGCEQASETKHDAANPVEENEWDKLLRLRWEQYQIEEEASLGRGKRLRKAVSYRETFAAIANETLSENSDDGDEPKREYTAAGLALKEKYGKLRARQKERIAQRHTIKNYADDKLEDFIKLYDSSVNEYGVNPLRIVEDPDSVQPSGAKRFSDSTAEMRHSSKKAKRYPEISRELHAKLAAHATSSKHHSKATDVLNQGTPHHLLPVLGLCAPNADQVNSYKNSNCGPNMKEQKRASSEVSNRPLSPAAEHSSELKNHGQSAPSKTMFPGSSETLRRLSNILPDSYFPFHPIPPISGKGLCDPVENPLPSIASCQGKMGLPNFCLEDNIPLNHMNSVPDLFPNFSLGTNKEHARNSIPELPNSSFLPNFMADIAGSSKKQRSKFMADMSALLPGLGISPVQPIHSAMPENHKKVLDNIMMRAQYATNKFMKKRSKPDYWSEDELDALWIGVRRHGRGNWDAMLRDPKLKFLNHRTSEELALRWILEEQKIMEEPMPVTRSSNSTFPGLSDAMMSRALSGSNFSKLKMEPPKLQSHLTDIQLGCSDIPSRFPHIEPSNYMTEGGPSLVPWKDFKNKTGYGGDFSGSTSDKWEKADIGLVPPYMPNPFMMESIGSLPINIPNGSSIQQNEFASSSHENILLHSVSDGQADLFHEMQQRVRLGKQPIEMNLYHTKHSIPLLETAGIFGSSRSNKLPHWLQEAVRAPPSKPIECELPATVLAIAQSVCLVLGEERPAIPPFPFQGPRLSHPKDPRNTPKKRRLYTVQQASTQAEHPKIVSGQGDQISAPEPKLMEAPLTSSIDQTNDAPSLNLNSPSSSSAGSQKQDVMPPAFEETHSTMEISEAIAAACTARSEAPETGCKRDGFTGLDGITSGSCRSPMGNAPDAGAPRRELSGPGSPPAEFSLLPVVDATGLSTTWAVGPVSSDDQEPEQENLLDSDRHIGDMEKLEKPTPLDESRDSGASQSVSAQIADEDKVDMVADEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WK47</accession>
    <name>A0A8I6WK47_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123441998</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974073.1">
        <property type="nucleotide sequence ID" value="XM_045118138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WK47"/>
    <dbReference type="SMR" id="A0A8I6WK47"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063870"/>
    </dbReference>
    <dbReference type="GeneID" id="123441998"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0050850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0050850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0050850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441998"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000061">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF2">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-14S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="209"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="601"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="292"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="875" mass="96672" checksum="C3F82835B6B4CCBF" modified="2022-05-25" version="1">MGADPDASSPSPPPTSPLRQPRQGEEELCAVESVDHRSPSRHTDSNPAPQPSPASAAPVPQHPEVSGEEAVLASEEVVEEQPAVEEVVEEQPAVEEGVVEAEAAVAGGEGEALRSFLEEFGDQADDCLIPSPRLKGIATPDCPAALQFLGGRYNILMDKHNILMERYKQQVAKCAEECAPRFDGLMKKYTAECAERRRLYNELIELRGNIRVFCRCRPLSSDEISHGCSSVVQVDPSQEMDLQFVPTEKERKTFKFDHVFGQADDQEAVFAESLPVVRSVMDGFNVCIFAYGQTGTGKTFTMEGVPENRGVNYRALEELFRMSEERSSSVSYSFGVSILEVYNEKIRDLLNENSEQTSKRLDIKQSADGAQEVPGLIEAPISTIDGVWEKLKAGARNRSVGSTSANELSSRSHSLVRVTVTSEHLVTGERSRSHMWLVDLAGSERLAKTEVEGERLKEAKFINKSLSALGDVIAALASKNAHIPYRNSKLTHLLQSSLGGDCKTLMFVQISPSSADSGETLCSLNFASRVRAIEYGPARKQVDPAENFKLKQMAEKLCHEEKENAKLNESLQLMQLKYASRESVFRALQEKIRETEQACRTHQQRARELENELANEKKAARDTNKSTKPSFAAPVRQRPPLAPMRQRPPSNNMPQPSGPSRLRFAGKGSSAQNKENVPMTNKTVVDKAVGKARRVSLVPTMRQIPLQPKRRSSIAILPSERERMSIFPEKAMSRLSHVQMSRTARPQATFNSIPETPQPAVHATPDVRGKFIRMEFGSSSSSRFSSPPALSMRKSRNNILSPQQKLRLQSGSGNASKLCFSIQKRVALGSPAPARRTSVVSGTGIFNRSLREQMMAGRFGNAQQVFNSKRRMSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WL47</accession>
    <name>A0A8I6WL47_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS05G0509800 PROTEIN"/>
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    </dbReference>
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        <location>
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            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="105" mass="11592" checksum="F264118C7150477F" modified="2022-05-25" version="1">MDTEPMRCACCDFKEECTQEYIKSVKANFGGEWLCGLCSEAVGDELNREGRGQDGVQEAIKAHMAFCRMVLSSPAVRVADGMREMLRRSSRDKGRSSTSAKSCSL</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I6WM53"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130870.1.CDS1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0130870.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130870"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545:SF36">
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="504" mass="56024" checksum="F41ABF4970B0748C" modified="2022-05-25" version="1">MAKQKLRRLVRGPRRAAGEDRLSALPDELIGLIVGRLDTRTALSTAVLARRWARIPRDLPALDFRVSDILPLEYDQGVALREENLPPDTAMAAMLDGFLARCEMGSTRALADGVTSFLAADRDGDAHRRVKALRLEFFPADHGGCIERLIAAAVGSWGVEDLEIVVRPASRSRHHDGPAYHFPDDCLDDGGRSRLRSLTLGNTTLPPLHSYEALTTLVLQGMAASTPVAVYGRLFNDLTRLQVLHLVSCSCTADLDHLVVDAPGSAVRELVVEECAFSSITVRALPALARLACIGTIVVLSLDSVPNLTQVNLTFWGREKPSNFLGKAPAATTSLVLRFTGPSRWVVPLHLATPFLGLKRLLVADLPSNWDVSWPRMLVLAARSLEVLHIHVAHSDEKQGEEIMWWHWPIPKSKLRHRNMKEVVMIGFTQTSRQIKFLRDLLKMCRSLQRVLLLRDGQVRYNGLWDWKMVGQPEPGCPWSTHDKMVVTRMIKPASRPLVDVILG</sequence>
</entry>
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    <accession>A0A8I6WNI7</accession>
    <name>A0A8I6WNI7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
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        <text evidence="1">Belongs to the peptidase C48 family.</text>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="NPH3 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR36479">
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        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0109910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="148" mass="17329" checksum="1EC80BE012F6F621" modified="2022-05-25" version="1">MFPVIEEMGGKKVSGHHWYVLCINNTAQRFEALDSLRSKGSIGLIYHANAVMRRIKEAWKLYYDKSRVQIENYEIEIIDAPKQAVRSVHCGFYMLEYLDKWDGVNVPRIRKEDIQKIKKITANKWLNAPFNKNKKWKQQLDSNCPGGK</sequence>
</entry>
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    <accession>A0A8I6WQ33</accession>
    <name>A0A8I6WQ33_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ33"/>
    <dbReference type="SMR" id="A0A8I6WQ33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173820"/>
    </dbReference>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="166" mass="19573" checksum="03D6EE949C48BF5D" modified="2022-05-25" version="1">MKFCLSMLDETTIETPRFKFKSMHNIVHIDEKWFYMTKRNRNYYLLDGEEEPTRTILSSNCIGKIMFLTVVARPRWDSEGNVMFSGKIGIWPFVKEVPAQRKSNNRPRGTIETKSIKVDRKVMREFLIENVLAAIQVVWPESDVGRQSIYNMTMPSSTYYLLTRNF</sequence>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <accession>A0A8I6WRM1</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A8I6WRM1"/>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
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    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
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    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="154"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
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        </source>
    </evidence>
    <sequence length="380" mass="42330" checksum="88E1F989BF5FB573" modified="2022-05-25" version="1">MHAGAAPEIDQEGFELPRRKDRRRHSRRAAAQDPPRPRSPSAEEAAGLCFLCLDLRHRVRDCTGDGRAAGAAAHHGDHHTVAAPPMETPDRPETSSTPVRVILARSVEMEETEGVLHRAMVATIIGTRPAVSAKEVSELLCASLDMHPGDFSVHLHRPEDFLIVLASRELKDRLAGDHFISGPRFSLSLRPWCKLAHVGSARLTYRVEIELRGIPADAWHLSTEEHILGSSYWIKRLHPSTRSRADLATFRLSARTMDPANIRRHAILEIVEMHPSRHQSDAPAISTLSYPVSVMLARAELDAPAPCSVTRADDPDGGQGGDHERHDNDPARCACQRRRGRKRRRTSSTPADRADGMQMDDLPWPLNPQHRHGGWLDLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1X9</accession>
    <name>A0A8I6X1X9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X1X9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141:SF106">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="180"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="368" mass="40205" checksum="14FB20A7D44EC827" modified="2022-05-25" version="1">MAGQPPFTNVDPATEADLRRRGLDPHEVDRSTEQLLTHICTELVPALPLSDPRRHTPLQRILSRLVVTDTARSPAIPAPDGVDRISLLRDELLREIVCRLPVKDGARTSVLASRWRRVWLSTPQVLADAHLRPKGCSLTPTPADSPAIIAAVSSILEAHTGAIPTVHLTCSPANAYQVQLARCLQLLATKGVQELVLVNRPWPLDVPLPPALFGITSLVRIYIGLWRFPDVARLPPRTSFPHLRELGICSVVMEDGDIDFVVAMSPILETLNIHGCNKGLRLRLVSQSLRCVQICSSVLEDIAVVKAPRLERLILEGFRSNAGGLCTRVRIGDAPKLHALGILEPGNTMLEIQDTIVMVQTSPVCSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3P7</accession>
    <name>A0A8I6X3P7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3P7"/>
    <dbReference type="SMR" id="A0A8I6X3P7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0226210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0226210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0226210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0226210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000213">
        <property type="entry name" value="Coniferyl alcohol 9-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="25"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="136"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0226210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="28552" checksum="170509D9C992C08D" modified="2022-05-25" version="1">MAAESSQTMTTPTDAELVQAQADLWHHCLSYLTPMALRCAVQLGIPTAIHRLGGAASLPDLVTALSLPTSKVPFVGRLLRLLAATGILASNEAGMYSLVPLSYLLVDGVRIDGDASQTAFVLTVTSRHFMEAAIGLADWFKNDIPPPVPSPFEDVHGAALFEESMAALDPECDKLFNESLAAHDHMGIGTILRECHGIFNGLQSLTDCCGGDGTTARAIVKAYPHIKCNVLDLSRVIDKAPRDGFVNYIAGDMFHSIPPAQVVMLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4B9</accession>
    <name>A0A8I6X4B9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4B9"/>
    <dbReference type="SMR" id="A0A8I6X4B9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0246050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0246050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0246050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0204240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0204240.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0204240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0246050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0246050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0246050"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0246050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="117" mass="13339" checksum="C503F6E192E07C62" modified="2022-05-25" version="1">MFGTSFVPKSIWQVYSWLYAFLPGFCDIYTIGLAAVCWAMWLARNRATSEKKWINTPFEIVFTACAFLEYWAGLQKPMMTEVVKKGDEMLKENASQMLLLCGPPRPGSTEHAEEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X536</accession>
    <name>A0A8I6X536_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">DUF295 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123442942</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975068.1">
        <property type="nucleotide sequence ID" value="XM_045119133.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975069.1">
        <property type="nucleotide sequence ID" value="XM_045119134.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975070.1">
        <property type="nucleotide sequence ID" value="XM_045119135.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975071.1">
        <property type="nucleotide sequence ID" value="XM_045119136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X536"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0237820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0237820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0237820"/>
    </dbReference>
    <dbReference type="GeneID" id="123442942"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0197420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0197420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0197420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0237820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0237820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0237820"/>
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    <dbReference type="KEGG" id="hvg:123442942"/>
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        <property type="component" value="Chromosome 3H"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box_BR-signaling"/>
    </dbReference>
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        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259:SF91">
        <property type="entry name" value="OS01G0183800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS07G0183000 PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="78"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0237820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="41500" checksum="CDFD4D7E306BFF64" modified="2022-05-25" version="1">MSTKETRLKEVNSVGMKDWANIQTDIGGLIIKKLGIPDYIRFRAVCTSWNHICKEVSNHPRVDPWLMLPAKPLDGAKFFSLPEKKSQTIHIPSVATIFGSMWTPVGSSHGWLIFFNPPQGTIQLVNPISSASFQLPSVGRRHLSKAMLLDMSESNFTVAVIYRDQKGYKVTRKGSKSWSFVDSKHILVDVFKHRRQLYTIDVYGTVEVWAEPPRSWPDEDFPQVEPHMHNLVHYQHQKFNCLVETPSGDLIRVKRQSQNMFALWVLDRGTSTFEMTSDIGEFALFVSHYSSFCFPAKDHPYLKANCVYFIDSYNNLCAFNLEHGTKELVEALETAAPTRSQQDVYGRHPRAEPFLWLYPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9D5</accession>
    <name>A0A8I6X9D5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9D5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161250.1">
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="27009" checksum="DC1621F533C07941" modified="2022-05-25" version="1">MATFIPVKFTEFCQPDATMPFGKELVQITKDLRIAPPTITGKPTSSYPEDSRYRIKVHVPGRTFQPRTEPMDFKFIAPNWILGRDMAIHCALGRIKEEYKGCHISPDLFTVSRRNEDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLRELELEEAAREAHYEHELEVKDFLERIEKLKTRVAYLEEELDMGENLHPEGDLAPLISNDEDYEESSTKGPTTMLF</sequence>
</entry>
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    <accession>A0A8I6XBE1</accession>
    <name>A0A8I6XBE1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123424414</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044963963.1">
        <property type="nucleotide sequence ID" value="XM_045108028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBE1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0100390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0100390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0100390"/>
    </dbReference>
    <dbReference type="GeneID" id="123424414"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0082770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0082770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0082770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0100390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0100390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0100390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424414"/>
    <dbReference type="OrthoDB" id="435038at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46158">
        <property type="entry name" value="OS02G0165000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46158:SF1">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="252"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="223"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="467"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0100390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="516" mass="54171" checksum="CA69A15B6A23A830" modified="2022-05-25" version="1">MDSPRMEQESSGGGTGSATITNPMQGGVGGGCPAPDDQQAKRPPHLSIDIPAASPSSVPPTPTASAEAEAVAATPGSNASRTPGSGSGSGSGKNGAPAKPPAPQRTPSFMLRQTVRSLLPGGGSFKSSVRGYEASLSRLFSGRIARTASLPAVDDGALSASAHGAGDKTQQSVPAAAADKTGMHRSQSLPMNMKKLSSAKSIKRMNSLGGVYRVVPSTPRAPAAAAATSNAAPDIVPTEPGAGEEEDDHGEDIAEEEAVCRICMVELSEGGGAMKLECACRGELALAHTDCALKWFGIKATRTCEVCKEEVKNLPVTLLRVQSTRGGGEAATRAGANRPRYVRYRLWHGTPILVVISILAYFCFLEQLLVAHNGFAALAISLPFSCILGLFSSLTTTSMVARRYVWIYAAIQFLFVVFFTHLFYRYLHLQAVISIILATFAGFGVGMIGNSIIIEVLRWRTMAPAQQRRARRPPRVAQQQQQPAPAHSQPSGQLSDAGEGQRSATVDIENPAAPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDZ9</accession>
    <name>A0A8I6XDZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0324890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0324890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0324890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0324890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0324890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0324890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF25">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="201"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0324890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="416" mass="46994" checksum="0844345873BC209E" modified="2022-05-25" version="1">MEEELCLVDSGTTNSILREINFFQTLKKMNGDILTIAGRDTVIVGTGRAIFTLPSGTQVTIEDALLYPDSSRTLISYRDIRKNGFHIETHEDNKEEYLLFTKDHGYGKKVHEKIPSLSSGLYYTYIKPVEHVAYKVIFQNVDAFQTWHGRLGHSGIGMMRKITSNSIGHNLLESKFPQSSVFVCTSCATGKLILRPSHLKIQAEPLQFLERIQGDICGPIQPLSGPFRYFMVLIDASTRWSHVCLLSTRNHAFAKIMRQVIKLQAHYPESRIKTIRMDNAAEFSSRAFNDYCMALGIEVQHSVPYVHTQNGLAEALIKRIKLIARPLLMNCNLPTSCWGHAVLHAADLIQLRPTAYHSSSPLELVRGNPPSISHLRKFGCAAYIPHHHSGHLWAHTESWGSTWGTNHRRLSSIWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEW3</accession>
    <name>A0A8I6XEW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small nuclear ribonucleoprotein Sm D1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">snRNP core protein D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123426973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in splicing regulation. Facilitates post-transcriptional gene silencing (PTGS) by limiting the degradation of transgene aberrant RNAs by the RNA quality control (RQC) machinery, thus favoring their entry into cytoplasmic siRNA bodies where they can trigger PTGS. Does not participate in the production of small RNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966829.1">
        <property type="nucleotide sequence ID" value="XM_045110894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEW3"/>
    <dbReference type="SMR" id="A0A8I6XEW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174570"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174570.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174570.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174570"/>
    </dbReference>
    <dbReference type="GeneID" id="123426973"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0144300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0144300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0144300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174570.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174570.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426973"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01724">
        <property type="entry name" value="Sm_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000008">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034102">
        <property type="entry name" value="Sm_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12710" checksum="FCA8BE65614CBA2F" modified="2022-05-25" version="1">MKLVRFLMKLNNETVTIELKNGTTVHGTITGVDISMNTHLKTVKLTMKGKNPVTLDHISVRGNNIRYYILPDSLNLETLLVEDTPRVKSKKPTTGKPMGRGRGRGRGRGRGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XF42</accession>
    <name>A0A8I6XF42_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123427497</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967501.1">
        <property type="nucleotide sequence ID" value="XM_045111566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XF42"/>
    <dbReference type="SMR" id="A0A8I6XF42"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0187920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0187920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0187920"/>
    </dbReference>
    <dbReference type="GeneID" id="123427497"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0155610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0155610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0155610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0187920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0187920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0187920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427497"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044607">
        <property type="entry name" value="RKD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373">
        <property type="entry name" value="PROTEIN RKD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373:SF36">
        <property type="entry name" value="RWP-RK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="9">
        <location>
            <begin position="183"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="272"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0187920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="340" mass="38638" checksum="8522891DDA1EF3D9" modified="2022-05-25" version="1">MDAAVTTLSALAVFASTLHQGAVRSVHGYRVVGVGGGRLWDRWVEREFAFSPSSCREVPLPAAAPRILPVEWRGRPAYREGQVLGAWRCVLAFDSLVAAALPPTPPPMLCPFRNPGLVCVPSLYSDLYMVFQFQRFQQAPQLVQRDSDEKPTRLDAEDNKTSDEVEEESGSDSDEDPQSGEELSAQVERRRRAKKQHIASITLVDIAPYFHLPIREASRALKIGVSILKKKCRQYGIPRWPHRKIKSLDSLISDLEFVMDDTDGDAVQKETCKQREKEKKKEEKDKQNAIQALAKRKRLLESEKETIQQKPALDLMAETKLFREDVFKRRYRAKRLPGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XH94</accession>
    <name>A0A8I6XH94_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123444857</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977656.1">
        <property type="nucleotide sequence ID" value="XM_045121721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XH94"/>
    <dbReference type="SMR" id="A0A8I6XH94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0301000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0301000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0301000"/>
    </dbReference>
    <dbReference type="GeneID" id="123444857"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0301000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0301000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0301000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000025">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000360">
        <property type="entry name" value="Os01g0533900 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF18">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="579"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="623"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="702"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="726"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="805"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="244"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="583"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="904"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="499"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0301000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1147" mass="124635" checksum="658BC9C6A477E530" modified="2022-05-25" version="1">MYLKAILRQDIAFFDKEMSTGQVVERMSGDTFLIQDAIGEKVGKIIQLLSTFFGGFIVAFVRGWLLALVMLSSIPPVAVAGAIVSRMMTTLSTEMQAKYGDAGDIVEQTIGTIRTVVSFNGEKQALTTYNKFIRKAYESARREGAVSGLGVGSVMAILFCSYGLAVWYGSKLIVDRGYNGGIVINVIMSVMVGAMSLGQAAPSITAFAQGQGAAHRMFKTIERRPCIDVYNTTGIIMEDIKGDVELKDVYFSYPTRPEHLVFDGFSLRVPSGTTMALVGVSGSGKSTVVSLVERFYDPQSGEVLIDGVDIRRLTLGWIRGKIGLVSQEPVLFSSTIRENIAYGKDDLNLEEIRRAIELANAANFIDKLPNGLETMVGERGIQLSGGQKQRIAIARAIIKNPRILLLDEATSALDMESERVVQEALERVMLERTTIIVAHRLSTVKNADVISVLQHGKMVEQGSHVQLVNKPEGAYSQLIHLQETLQQAETHKVDPDAIMKSGFGSRSFTRKPRSQGSSFRRSTSKGSSFGHSGTHPYPAPCVPMEVNNDQDLEETADKISSDQKKAPIGRLFYLNKPEALVLALGSIAAAMHGTIFPVYGILISSAIKTFYEPPAELLKDSRFWASMFVMLGACALVLIPIEYFLFGLAGGKLVERIRSLAFRSVMHQEINWFDKPEHSSGAIGARLSTDALNVKRLVGENLALNVQTISTVIAGFTIAMVANWKLALIITVVVPLVGFQAYAQMKFLKGLNKNAKLKYEEASQVATDAVGGIRTVASFCAEQKVMDAYEKKCEIPTRQGMREGLVGGLGFGFSFLVFYLTYALCFYVGAKFVHEGTATFPEVFRVFFVLVLATSGISRTSAVGADSTKANESAISVFEILDRKSKIDSSSEEGMVVASVRGDLEFQNVCFSYPLRPNVQIFTDLSLSIPSGKTAALVGESGSGKSTAIALLERFYDPSSGKILLDGVELPTLKVSWLRLQIGLVAQEPVLFNDTIRANIAYGKQGEASEEEIVAAAEAANAHQFISGLPDGYNTVVGERGIQLSGGQKQRVAIARAVVKDPKVLLLDEATSALDAESERVVQEALDRVMVGRTTVVVAHRLSTVRGADIISVVKNGTIVEKGRHEELMRMKDGAYASLVELSSTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI62</accession>
    <name>A0A8I6XI62_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI62"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28990" checksum="FE03F60B44E3D5CA" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVMVFPDYPGAKKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSDEEGYLSGVYFDSSMEDPLLQSTVEMLENKDKDDRALRMELDATRARLWTVLTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPSHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIB8</accession>
    <name>A0A8I6XIB8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123400006</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950335.1">
        <property type="nucleotide sequence ID" value="XM_045094400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIB8"/>
    <dbReference type="SMR" id="A0A8I6XIB8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497840"/>
    </dbReference>
    <dbReference type="GeneID" id="123400006"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0413030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0413030.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0413030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123400006"/>
    <dbReference type="OMA" id="TICKRSV"/>
    <dbReference type="OrthoDB" id="1546383at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035337272" description="Germin-like protein" evidence="13">
        <location>
            <begin position="27"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="76"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="23173" checksum="F2E5E1E72F75A463" modified="2022-05-25" version="1">MASINCCYSLVLVVVALVSAPLAAVAGDPDILTDFVVPTSMIGMPPMNITGDFFTYTGFGAAMNMTPMPMPMPGMQNFTVTKATMMEFPALNGQSVAYAMLKFPSESVNPPHTHPRAAELLLVLDGALSVGFVDTAGKLYTQDLAAGDMFVFPKGLVHYQSNPGQSHAVALSAFGSSAPGTVSVPVTVFGTGVDDAVLAKSFKTDLPTVQKLKAALTPPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJC0</accession>
    <name>A0A8I6XJC0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123446713</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044979204.1">
        <property type="nucleotide sequence ID" value="XM_045123269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJC0"/>
    <dbReference type="SMR" id="A0A8I6XJC0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392480"/>
    </dbReference>
    <dbReference type="GeneID" id="123446713"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0326520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0326520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0326520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0392480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446713"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000061">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000108">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1412">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 83A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="475" mass="51346" checksum="C7CA91C2296E9A72" modified="2022-05-25" version="1">MATSAEPASGHALFLPYPAQGHVIPFMELAHRFLDRGFAVTFVNTKFNHRRVVAAAGGATTTYTSAAGGRLRLVAVDDGIDDDDGDHENLILLNAAMQEAIPPQLETLLDGEDATGEGLGKVTCVVVDSGMSWSLDVVKRRGLPSAALWAASAAVLSVLVNAKKLIRDGVIDDDGAPVNLKNNSFHLNESATSMDATFLAWNYMGNRDAERLVFHYLTSTAQVAAAKADFLLCNTFSDIEPAVFSGPTPATILPIGPLRTWQRPTRHAPVGHFWHADDAVCMSFLDAQPGGSVVYVAFGSISVMTVAQLRELALGLETSGRPFLWVVRPEQAGKLPAGFADAIDGLGKGKVVGWAPQEQVLGHPAVGCFVTHCGWNSTLEGIRNGLPMLCWPYFTDQFTNQTYICDIWRVGLRVASADGGGLVMKEKVVELLDRIFKDEGAKERMLRLKEMAEKNMSEEGQSLNNMNVLMESMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJY8</accession>
    <name>A0A8I6XJY8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJY8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0446080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25902" checksum="EE86C67DA2328763" modified="2022-05-25" version="1">MSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEEPIHWSYGLGVTVDMAVQDAAYSILTIIRARHAVLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMEFYATRARLWTTLTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFRDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMV1</accession>
    <name>A0A8I6XMV1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ABIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123443914</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Binds SCAR.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976408.1">
        <property type="nucleotide sequence ID" value="XM_045120473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMV1"/>
    <dbReference type="SMR" id="A0A8I6XMV1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273340"/>
    </dbReference>
    <dbReference type="GeneID" id="123443914"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0226500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0226500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0226500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0273340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443914"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF0">
        <property type="entry name" value="ABELSON INTERACTING PROTEIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34397" checksum="83D3805123CE4212" modified="2022-05-25" version="1">MQQQQQQLQAPPSWVAGSDAARPAPATFDEASMERSKSFVKALQELKNLRPQLYSAAEYCEKSYLHSEQKHIVLDNLKDYAVRALVNAVDHLGTVAYKLTDLYEQQVSEVSTVELKVASLNQQVLTCQTYTDKEGLRQQQMIGNATRHHKHYIVPTAGNKRMQAFSEMQTDAEFDLKPKPYPSEKTLFWHLASEKNTKTNGEHQSELGHGETKTTKPTSSGGFNLTGKELSVSPLPKRLQSNVSSSDIVTRNIGMKDQPGARHLASFSSLDNPRGRQIQKAPVRTKSMLAAFFVRHRSGKMKNVSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XP68</accession>
    <name>A0A8I6XP68_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XP68"/>
    <dbReference type="SMR" id="A0A8I6XP68"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440710.1">
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF8">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17742" checksum="140E8B02D9A5C75C" modified="2022-05-25" version="1">MASSGSSIFSPVVAEVDGVGIVEHDSEFYLPDESYCGLEHSSKHRCPGHDRVPARRVTWGGANTGRRFLGCPLDLPDECHWVVWVDPPPPLHAALAFEDLHAELEQSWIRRHKMQKQNMELSKKNLALKKKLRERDEMLLVLALLFVGMCVVVFLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPZ7</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6XPZ7"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0408870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15695" checksum="51BDFB44369E4E50" modified="2022-05-25" version="1">MVLVSAFVGPRPVYDSDVEVCEPEIPTHIKILVEKLNLVEKGNKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHFAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQA2</accession>
    <name>A0A8I6XQA2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA polymerase III gamma subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444341</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976962.1">
        <property type="nucleotide sequence ID" value="XM_045121027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQA2"/>
    <dbReference type="SMR" id="A0A8I6XQA2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286720"/>
    </dbReference>
    <dbReference type="GeneID" id="123444341"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0286720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444341"/>
    <dbReference type="OrthoDB" id="1906110at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000013">
        <property type="entry name" value="DNA polymerase III subunit gamma/tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022754">
        <property type="entry name" value="DNA_pol_III_gamma-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054506">
        <property type="entry name" value="DnaA_N-like_STI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF46">
        <property type="entry name" value="PROTEIN STICHEL-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12169">
        <property type="entry name" value="DNA_pol3_gamma3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23007">
        <property type="entry name" value="DnaA_N-like_STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22608">
        <property type="entry name" value="DNAX_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DNA polymerase III subunit gamma/tau helical lid" evidence="10">
        <location>
            <begin position="498"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase III gamma subunit" evidence="9">
        <location>
            <begin position="547"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="STICHEL DnaA-N-like alpha-beta" evidence="11">
        <location>
            <begin position="740"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="63"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="713"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="629"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="716"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1049" mass="114867" checksum="3C386B3AF019D6E6" modified="2022-05-25" version="1">MPAPDRAAGGGHLRGHAHLANCVHLRHHHNAHGHGGGPLASSSGRRRSPASAALMRDLLALQRSRARTLRDPSTRRSVESSKVAADPDPYTDDDGGGGVDLPARSRRSAKADGALKTLLDQLADNPHPKAARRPRRRFKRGVGAGAARRAAGKAPDRAAASLSLNSSSQEAVCGNKYLFRDGEDSRNVCGIPWNWSRLHHRGKSILDLAGRSLSCGLSDPKSAEPEAAAAASRAHRSGSHSLFPVKSERLASSTGSDSDALPLLVEAATSGARSGIGRMTGSYSGELGLFSNRSSEMDSDLLSEARSGTRGSLRSRSRHRSLTQKFAPRTFKDVVGQSLVVQALSNAVLRRKVGLVYVFYGPHGTGKTSCARVFAKALNCHSAEHPRPCDSCISCIAHNVGKSRSVMEIGPVGNIDMDGIVDVLDNVMLSPAPSHYRVFIFDDCDTLPADTWSIISKVIDRAPRHVVFILVGPNLDLPHIILSRCQKFFFPKLKECDIVNTLQWISTSESLDADRDALRLIASRSDGSLRDAEMTLDQLSLLGQRISLSLVQELVGLVSDDKLVDLLDLALSADTVNTVKTLRDITETGVEPLALMSQLATIITDILAGSYIFARERPRRKFFKRSTLSKNDMEKLRQALKTLSEAEKQLRVSNDKATWLAAALLQLAPDKQYMVPSSSTSTSFNHGVLAGSLPGKDVARHSIIEHNGNMASTSYGERRTTEHTSNHHGSPTVTKVNERSKHSKTDNEIIWQAVLESIQSDTLRKMMAKEGKLGSVSLGTAPTVQLIFSSHVNKSKAENFRGQILQAFESVLHSAIILEIRYESKNDAGAGHDPSMFTHPENDSDMILRRSFSKHSPLSSGGETLIRRLKKDRVVKGASSTKTKWMQSDPHILTEGEIIEVGPSHMHRHPETDNAVPNINERRNDNVWEEALSSPNQEAVIKQGGKNGNKQRRQNSIVKRKLSLAHVLSKAEVCSQRGGWSRRKAMSIAEKLEQENLRLEPRSRNILCWRTSKTRRKLSSFRVRTGRSRSQAISRLILCGRCISTKSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XR99</accession>
    <name>A0A8I6XR99_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123449015</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044982011.1">
        <property type="nucleotide sequence ID" value="XM_045126076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XR99"/>
    <dbReference type="SMR" id="A0A8I6XR99"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0388220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0388220"/>
    </dbReference>
    <dbReference type="GeneID" id="123449015"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0322970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0322970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0322970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0388220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0388220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0388220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449015"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16987">
        <property type="entry name" value="ANTH_N_AP180_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000019">
        <property type="entry name" value="Clathrin coat assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000005">
        <property type="entry name" value="putative clathrin assembly protein At2g25430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF13">
        <property type="entry name" value="ASSEMBLY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="24"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="349"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="638" mass="70676" checksum="394E2EE57F0FEB24" modified="2022-05-25" version="1">MSSSTIRKALGAVKDQTSIGLAKVTSNIAPELDVLIVKTTSHDDEPAEERHIREILHLTSGSRAHVAAAVAGCARRLSRTRDYVVALKSLMLVHRLLTDGDPFFHRELLHATRRGTRLLNLSDFRDEAHSGSWDHSAFVRTYALYLDQRLEFLLHERKQGSNANGSTSLNGPSPRDRWGSPDPYGRRSPSYTSPPGNGYGGYDDNRERNGGNSDDKRPLTPVRDMKPERVLGRMHHLQQLLDRFLACRPTGGAKQSRMVLVALYQMARESFQLYADICEVLAVLLDRFFDMEYADCVKAFEAYASAAKQIDELCSFYAWCKDTGVARSSEYPEVQRVTDKLLETLEEFMRDRAKRPKSPPREPEPEPVKEEEPEPEDMNSIKALPAPEDYKEPEPEKVEEEVKPEPPPQPQGDLVDLREDTVSADEQGNRLALALFQGPPAAGGSNGSWEAFPSNGANEVTSAWQNPAAEPGKADWELALVETASNLSKQKAVMTGGMDNLLLNGMYDQGVVRQHVNAQATSGSSSSVALPAPGQKTQMLALPAPDGSMQNVGGDPFAASLTFAPPSYVQMAEMEKKQQFLTQEQMMWQQYQRDGMQGPSSLAKLDRTYNNGFGPNPAMPYGMPNAPMANTGYYYPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU57</accession>
    <name>A0A8I6XU57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123395430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="3">subsp. vulgare</strain>
        </source>
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            <end position="171"/>
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            <end position="92"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="220" mass="25081" checksum="0C41D9812A74EE2E" modified="2022-05-25" version="1">MASVALKLSRSWVAWTTTNSGRFALRLPGGDLAGNTTITARSRQLLRSPNSAAASTSTSDFFLRRSITTLSSGEDPRSPVGAAPDDLEDEFRPDPDTVPYFDEKDLVSDETLWALYERWCRFHEMARDHDEMARRFGRFKLKARHVYEFNKSGRSYTKGLTRFSDLEPGEFFAPRRCPRTPRIGSRFFTNDRGEVTAICTDGRLEKYEGPVWKIRNGKQP</sequence>
</entry>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34395:SF21">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS11G0427500 PROTEIN"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="9380" checksum="0B6ED2BB8D8F0833" modified="2022-05-25" version="1">MMEKYLEIRAKQVEDERNKPRVVDEYSIKNCIDLLKTMAITPEEEVKAFRVFKIPENREIFMSARPETALMWLRAEME</sequence>
</entry>
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    <accession>A0A8I6Y5J2</accession>
    <name>A0A8I6Y5J2_HORVV</name>
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        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5J2"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="CASP_dom"/>
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        <location>
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            <end position="82"/>
        </location>
    </feature>
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        <location>
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            <end position="110"/>
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        <location>
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            <end position="151"/>
        </location>
    </feature>
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            <end position="182"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0502270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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        </source>
    </evidence>
    <sequence length="216" mass="23408" checksum="9C6F5D17FA7A8CC5" modified="2022-05-25" version="1">MCSQTLLLHSTALPLLSIVKVHPSRARRRSFELVYQSARTMVAAGFVFVPGTWSGFALRVGQCGFSATSGLIMLTSLASLTYSRAYMYLIFVMVLHFVWSLVQAVVYLRTLLAGEDIHRPGIAFFLALGDGAVVLLTSSAVSASAMVTVLFVNDTGACGSFPELACDRYQLSVVLGSMAWFLQATSAFSNLCLMIKVATQQPHLVASDAFHVDRLL</sequence>
</entry>
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    <accession>A0A8I6YAT5</accession>
    <name>A0A8I6YAT5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
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        <name type="common">Domesticated barley</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAT5"/>
    <dbReference type="SMR" id="A0A8I6YAT5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647090.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0647090.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0647090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0647090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="95" mass="10709" checksum="97AD0B6CBA476F11" modified="2022-05-25" version="1">MSADQIFEFVRDANCYPNVSVAYCILLTIPVTVASAERSFSKLKLLKNYLTSTMSQERFNGLAMCCIKKNMLDSINLDTIIDDFASKNARKSHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YD00</accession>
    <name>A0A8I6YD00_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YD00"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712720.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="146" mass="17079" checksum="0A156FB66047CF6C" modified="2022-05-25" version="1">MKCVRSTRVVLNDLRENGWESMLAEVHVFCEKHDIVELDVEEAYVNPKKRRKVTEITNIHHYQVDCFNDAFDWLVQELDNRFSETSTNLLVGSATLSPRDSFHDFSLENLMSLAKLYPQDFDSGELRDLDKDLRLYIADVIELVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YE71</accession>
    <name>A0A8I6YE71_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YE71"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0678810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF70">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="1">
        <location>
            <begin position="658"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="857" mass="97962" checksum="535B5C7B9D987AA5" modified="2022-05-25" version="1">MHTLATICIYPAVYSPDIVPHIENVLHKEAAGVIDDTGMDGTNTETVLDCFLYSLFLTTRLPRNFTSVDYDWVTHACNYWICDGILGEDKTWEIGNTLYLLIPLLGNYTRERRRLASYLNGRKKTYVGWYSVTSNKLSAEDISNVPGSASSYFLTFQGDDPVYVRDDLFQLASNNLRVLKLYNCSFNFASPPFQCCHNLRFLWLDHCANTGKEQSGGTSFLNLLVLDLRFTDYVFLPQMIELMTNLRELNTKGVSWKTVSHAWKKLQKLQKLRLTESSDVVTVDSCSSVDTMNLELLDLSGNTHMESLPTLSSARSLKMLVLDGCSSLDHVALKGAPPKLESFSFDGYGLPDNWTHSIHLPQQELRLKSPLAPVEIVKVTKISLDGCGRLQNIFLRALPNLEELDLSGTAIKIIDLHAMDVPKLKKLFLLGCERLRSLIWQGLPRLEVLHVDTQRAKRSVVWCGEQGSFGFQAHIVFTDGRFIWSFSEALNSRSQNSFSKMYLLISCTSSSQANITKSIKEIGSSRQGLVPTRSLLPYNDIALAKDVTCSLLVWNRRQLQTLNVHIEIGKGSYNLESMQDEGHFGGFAEYVESVHVHDNCSITTIPPANWERLKWCHVERCPKLHSVFSRRNGTYRFRVINTFSASDLLVAYCIWESINKTWDDFFRYYETLQQLQHIYLYNCPRLVFVLPIYFTLPNLETLQIAYCSNLRHVFPRDSSNLRHVFPHDIASEYKYVEGTSSGFTFKNLKHIKLYHLHNLEQICEVRLFAPALRSVGIRDCWALRRLPAVAREGPKPLVNCEKDLWDRLEWDGLDAGHHPSLFETRHSAYYKKTLPRGSYLRSVARVFNYGKCSLTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFS4</accession>
    <name>A0A8I6YFS4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123404614</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044954491.1">
        <property type="nucleotide sequence ID" value="XM_045098556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFS4"/>
    <dbReference type="SMR" id="A0A8I6YFS4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542110"/>
    </dbReference>
    <dbReference type="GeneID" id="123404614"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0450880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0450880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0450880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0542110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404614"/>
    <dbReference type="OMA" id="TCRTNIF"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF88">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="110"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="165" mass="16973" checksum="0281F2CF9F23CA8E" modified="2022-05-25" version="1">MATPERAGRGNATATAIATNRWGPYSGAGDFAGNMAIILASLLAALALALALHAGVRCLLRRRRQRRGLDDGDEDPEKPPVAEEPAPPPPPLLVYSASGTRLAGAGAHECAICLAEFADGDAVRAMPACAHGFHARCIERWLAVPAAASCPTCRAPAAAPPPPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6YJ19_HORVV</name>
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        <recommendedName>
            <fullName evidence="10">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123403363</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953237.1">
        <property type="nucleotide sequence ID" value="XM_045097302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJ19"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579820"/>
    </dbReference>
    <dbReference type="GeneID" id="123403363"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0480840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0480840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0480840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0579820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403363"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="36848" checksum="34FF0BDA7899B4F0" modified="2022-05-25" version="1">MVSRGQRVSPSKSPPPFRKTAKPFYAIPHPRRKPALQTRHPAPRQEHTAQHSAGTVWESGSGEGEPPAMAVSNNITACVTLMALICAVPVIASGVWFASAQGEECARLARWPVAILGGLILLAALAGFVGAYWNRRRLLAFYLFAMGALIALLIALLVFAFAVTRGSGAYPVLGREYDEYRLDGFSMWLRGYVSDDPARWEGIRSCIAVSDTCKKLARQASFVTADQFYQSHLTPLQSGCCKPPSVCGFGYVSPTVWTNPARPAADPDCGLWSNDPAQLCYECESCRAGLLAALRSQWHKANIALVVATVSLLFLYLIGCSAYKNAHAEAIYRRYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJJ7</accession>
    <name>A0A8I6YJJ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJJ7"/>
    <dbReference type="SMR" id="A0A8I6YJJ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0403210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0403210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0403210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0403210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0403210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0403210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="55"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0403210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25176" checksum="FCAEE6755A75B169" modified="2022-05-25" version="1">MPTRFEEVLAHGTTMLDVVYTNESREMPFFLEQLNERWLDAAMDHEKFLGLNLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRSGITFATVDIRNDKLKMRYSFGIEIPTGCLIDLQTVFMLRHVRTSMAHMAVALIDEEYDDMKTSFPKSQHKLWEKAPLDRINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YK69</accession>
    <name>A0A8I6YK69_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="1">
        <location>
            <begin position="42"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0597180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="146" mass="17409" checksum="90E064A7FA74C4A2" modified="2022-05-25" version="1">MHSSMNQNYSRRSRNEDDFVHFIFPTLQGSSSQSSERRAIHTSILNGVTYVYEALTGHEVLCEWRFHMERSIFQVLVQQLYEKHLLQDARFQYGADSVSTYFHAVLNAITRLTCNFIQPSIYQHPILRQPKFLYFRIKSTELYWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKN6</accession>
    <name>A0A8I6YKN6_HORVV</name>
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        <recommendedName>
            <fullName evidence="15">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123410821</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="13">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959697.1">
        <property type="nucleotide sequence ID" value="XM_045103762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKN6"/>
    <dbReference type="SMR" id="A0A8I6YKN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747790"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747860.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747790.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="hvg:123410821"/>
    <dbReference type="OMA" id="WYNIDKA"/>
    <dbReference type="OrthoDB" id="610659at2759"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Reticulon-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF742">
        <property type="entry name" value="3-BETA HYDROXYSTEROID DEHYDROGENASE_ISOMERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
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        <location>
            <begin position="15"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="370" mass="41122" checksum="526519AFAF02C3FF" modified="2022-05-25" version="1">MDPSPASQEARRLCAVTGGRGFMARHLVAALLRSGDWCVRITDLGPDVAMEPAENDGLLGAALRDGRAAYISVDVCQLAQLTEALEGVETVFHTAAANQINNNFQLHYKVNVEGTKNVIEACNTCKVKTLIYTSSSGVVFDGVHGLFDADESTPYPDKFPDAYTQTKAEAEKLVMRANGIDELLTCCIRPGSIFGPGDTLVPTLVSYGGIMIIIGDGKNCDDFVYVENVVHAHICVEKTLSTEDNAKRSGGKAYFITNMEPVNMWDFLYMVLEELGYKSHFRLRIPLCLLKPITFLVDWSYNNILSHYGMRQPHMLTSTRIKYVTLNRTFNCNNAVEQLGYKPIVSLKEGVNMTTEFYKCFRARDMHISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPB8</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0699360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="148" mass="16413" checksum="B0BF91A7EAA7CF81" modified="2022-05-25" version="1">MQQIFSFGLATSKHAMGSGQPLGSPMPEYPDTQESNGINIDAHEKDGEHVHVLDSKTKRASFVDEELNIFNSMTKAVKEVATAMRESKSMDVHPQLYGAIMKRTCFSPEALMVAISHLLDNKAQGVGFVAMGPAHRVLWLRTWLGKHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQI5</accession>
    <name>A0A8I6YQI5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQI5"/>
    <dbReference type="SMR" id="A0A8I6YQI5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0545860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF89">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035247580" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="14">
        <location>
            <begin position="24"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="893"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="16">
        <location>
            <begin position="208"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545860.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="953" mass="105776" checksum="B4E3AEDCA8449BBD" modified="2022-05-25" version="1">MDMLQLSCIQITIALLLFTQAKSTTKEISALHPDDAVPSCVAGERSALLAFRAGLSDPGNLLSSWKGDDCCRWKGVYCSNRTSHVVKLDLQGSDDYRQALAGNISSSLLGLQHLRYLDLSDNGFDKIQIPEFMGSLHQLRYLDLSSSQFIGRIPPQLGNLSNLRYLNLESYSDTYSTDISWLSRLTSLEHLDMSQVNLSTIVHWLPVVNMLPTLKALRLVGCQLRSSPDSIQFSNLTSLETLNLSDNDFHRSSTPNWFWDLTSLKYLDISFNGFYGPFPDEIGNMTSIVELDLSKNNLVGMISSNLKNLCNLEGLVSLGNNINGSIAELFHRLPNCSQNKLKDLFLPESNLTGSLPTTLLEPLKNLSVLYLSDNKLTGHVPVWIGELTQLTDLGLDSNNLDGVIHEGHLSRLEMLEELRLSDNSIAITVSPTWVPPFSLVIIELRSCQLGPKFPMWLRWQKHASSLDISNTSINDMVPDWFWIAASSVGSLNIRNNQITGVLPSTMEFMRTREMDFSSNLLGGLIPKLPINLTDLDLSRNNLVGPLPLDFGAPGLATLLLYDNMISGAIPSSLCKLQSLRLLDISKNNLKGSISDCLVNESSTNMTDLSIVNLSLRDNNLSGDFPLLLQKCTQLIFLDLSNNQFSGTLPGWIGEKLSSLSFLRLRSNMFHGQIPVELTKLVDLQYLDLAYNNLSGSVPRSIVNCTGMTQRRDNDDLRDAFSAGVYSAGNYLVDYTENLTVLTKGQERLYTGEIIYMVNLDFSCNSLMGEIPEEISALVALKSLNLSWNKFNGKIPENIGALMQVESLDLSHNDLSGEIPSSLSTLTSLSRLNLSYNNLRGKIPTGNQLQTLEDPASIYIGNPGLCGSPLSWNCSHPEQVPTTRERKGDAMSDMVSFSLATGSGYVMGLWVVFCTFLFKRRWRAAWYSLCDNLYDHVYVQVAVTWASFRGRING</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRN0</accession>
    <name>A0A8I6YRN0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Early nodulin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRN0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0649590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0649590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0649590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0649590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0649590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0649590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005050">
        <property type="entry name" value="Enod93"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605">
        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605:SF2">
        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03386">
        <property type="entry name" value="ENOD93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0649590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="21834" checksum="FC7179774B8BDBC8" modified="2022-05-25" version="1">MDPYHSFGYSSALQVQTSEQREHPRFPPKTVRGGHLFARYKYMASSSSWRHQATTSQAAHLISLYRTPCTEPFLYKITAKMSSTATVTGANPENKLALAKRCSREATLAGAKAAALATVASAAATLASVRMLPWAKANLNPTGQALIICTVAGMAYFVAADKTILSLARRHSYESAPDHLKDTSFHGAAPRPRPPPPAFFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YSH5</accession>
    <name>A0A8I6YSH5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123407209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956223.1">
        <property type="nucleotide sequence ID" value="XM_045100288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YSH5"/>
    <dbReference type="SMR" id="A0A8I6YSH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657320"/>
    </dbReference>
    <dbReference type="GeneID" id="123407209"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407209"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000048">
        <property type="entry name" value="Protein strictosidine synthase-like 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF112">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE CONSERVED REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035257407" description="Strictosidine synthase conserved region domain-containing protein" evidence="5">
        <location>
            <begin position="32"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="165"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="348" mass="37784" checksum="3FAFF0A91AFFD204" modified="2022-05-25" version="1">MASGTRGVAAATISLAVLLLIFCFSPSTAAAAAVPSIDATRTRHLPLPRGLLRGPESVAFDAKGHGPYSGVSDGRVLKWNGDALGWSTYTYNPDYSSEACTASLLRPETATESHCGRPLGLRFHLKSGYLYIADAYKGLMRVAPGGGEATVLVTEVDSVPLRFTNGVDIDQVTGEVYFTDSSMNFNRSQHEMVTRTGDSSGRLMRYDPRTGKAVVLQAGITYPNGLAISADRTHLIISSTGPCKLLRYWIKGSKTGTMELFADLPGYPDNVRPDKKGGYWVALHREKNELPFGVDSHLLALRIDAEGKIIEEMRGPKSVRPTEVVERKGGKLFMGSVELRYVSVVIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVE0</accession>
    <name>A0A8I6YVE0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVE0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0687040.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0687040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0687040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0687040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0687040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="189" mass="20373" checksum="D8EEC1366C50C73D" modified="2022-05-25" version="1">MEDTRRTAAAASGDVLEKPGTTTATEAVQDVAGIQGGVGEAASVIAAVDLEKELLEQEVTASSGALQAGDEEEKAPEGKKTKLDMTGFKDPYDPHFVDDEINEYGSAGDVDKGNVEPFIAKEVEKIKAKGSSLYYKRKKDVCPYCTTKEYLKDGIYEDLLSHARDASMSSEDYKVRGQHAALLKALVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YYA6</accession>
    <name>A0A8I6YYA6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YYA6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0541850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="3">
        <location>
            <begin position="49"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="781" mass="88498" checksum="D271C1F26D7DAEAE" modified="2022-05-25" version="1">MGLSSAVDWWEEWQMRILVLGSLCVQFLLLCSAPFRKYAIPSWYRSIVWLAYLGGDAIAIYGLATLFNRQRRQDSGSANSSTSILEVVWAPVLLMHLGGQDHITAYNIEDNELWTRHLLTAVSQITVAVYVFSKSWKGGDKRLLQAAIVLFVPGILKCIEKPWTLKNASINSLVSSSDPQLMTAAGKSISLEEYVEGAIAFAQDDHVPQAQGEARADDLEANQHLQAHAEDHSDDPKPNHAPKIEHKLSTLGDMQQIDQDIINTRLRELLNLDQHINIRHPSHLFVDIASSFSDRLCVLNAFRTHYGEKAYELLQVGLFSMYDLFYTKQTMLSLPSGPIRHPWLLLCGYFLRIACLTLPYAAIGLFHSSHREAYNGHDVKVTYALFCCTAMLETYSIYAKMILILKLAASELSTEKSCVSVSQYSLIGFFARNIRHTIKMCVLSFVQCKDFLDQRWCMKPCKSSIALTELVLEYVKKGWKDQITDVESYWKFIDHRGEWTLQNKCEQDLIWSLRRPFDESVILWHIATDFCFFRCKAYPHERAREMSNYLTYLLFVKPEMLLPGTRCNLFKTANDELEAIVKQVKGDKLSLKAILKGDKPSQDILKGSKSFIQFLTGEKASQDEVEKGLMEIIFAKMKEREEVASCTELPLDADKNPAAEGLINNAWKISEALLAIDDESKMWNVIEGVWVEMLCFSASRCRGYLHAKSLGSGGELLTYIWLLLSHMGMETLPERLQRTELSTEEGNASIPPSTSQIPGSKDAPPFRRSRLHDTGARNTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z7B1</accession>
    <name>A0A8I6Z7B1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z7B1"/>
    <dbReference type="SMR" id="A0A8I6Z7B1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0727200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF37">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27428" checksum="6C3BAC70E8913F23" modified="2022-05-25" version="1">MDQVRDAPPSGTVESDYETIAGMRYKEMVASKGKPFPFKHAWAMLQTFDKWKLRDQETAPKKSAMLRMDDSEDEEEERNLGKPEGTKKGKQRVKMEGEASSLREKMEQMMKAREELTRKTLETKLLITEKKKEVKLAQVEARREDAKRKADLEERMIKVKEAKAWKELMVEEKEHMMMSKKDMDEDQLMWWKEYKEDIVERKRIFRGASSTLRGDTLMSGGGDGGVEDSTTGYNGGA</sequence>
</entry>
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    <name>A0A8I6Z7J4_HORVV</name>
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        <name evidence="7" type="primary">LOC123407453</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the HIPP family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045100589.1"/>
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    <dbReference type="SMR" id="A0A8I6Z7J4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748070.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        </location>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <fullName evidence="6">Thaumatin-like protein</fullName>
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        <lineage>
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    <reference evidence="5" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045102807.1"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0752030"/>
    </dbReference>
    <dbReference type="GeneID" id="123409938"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0752030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409938"/>
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    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035265259" description="Thaumatin-like protein" evidence="3">
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            <begin position="21"/>
            <end position="173"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="85"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0752030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="17580" checksum="ECBFA8833F21E5A9" modified="2022-05-25" version="1">MSTSAVLFLLLAVFAAGASAATFNIKNNCGSTIWPAGIPMGGGFELGSGQTSSINVPAGTQAGRIWARTGCSFNGGSGSCQTGDCGGQLSCSLSGQPPATLAEFTIGGGSTQDFYDISVIDGFNLAMDFSCSTGDALQCRDPSCPPPQAYQHPNDVATHACSGNNNYQITFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1M0</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="5">Tropinone reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I7B1M0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0086570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0086570.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0086570.1">
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="adh_short"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0521">NADP</keyword>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0086570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16026" checksum="91B830F08269E401" modified="2022-05-25" version="1">MTSGREKRWSLAGATALVTGGSKGIGRAIVEELAGFGARVHTCSRNAAELEECRRQWEEEKLAITVSVCDVSVRAAREELMETVRESFEGKLDILLEPDALEQACSRIPMGRSGKPTEVASVVSFLCMPTASYITGQVICIDGGRTIY</sequence>
</entry>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0193850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0193850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0193850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0193850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0193850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0193850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0193850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="13614" checksum="1974472BEF2A2841" modified="2022-05-25" version="1">MYPVGVVVENQLRVWAMSRWRGGREFAGLFLRALYRHLPVGTPQMFTIDESCMGGTGMIVCFRNPYDAYHLLGHVWFGGCEFICFTTYNIFTDLDSIWPNEHAMHTLPYNLPADDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8S8</accession>
    <name>A0A8I7B8S8_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971792.1">
        <property type="nucleotide sequence ID" value="XM_045115857.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I7B8S8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219120"/>
    </dbReference>
    <dbReference type="GeneID" id="123439108"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0182430.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0182430"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS01G0111000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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            <begin position="21"/>
            <end position="181"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="174"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="181" mass="18850" checksum="A45A85FED8ADFBEB" modified="2022-05-25" version="1">MASTATTVAMALVVLAAVSSDVLHVADADAGLIASTCKKTKNPATCVAMLSADRRSAIAITVHGLANTALLIAIDTVYNNTAAIIDLYQNLEGTPEGGALAVCNDAYLEADNDLELQARLALDYLNYAGASRVILLAKGAGDACENAFKAINKKSPLTDMDRQMTERCGVTADLMDLLASK</sequence>
</entry>
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    <accession>A0A8I7B8W9</accession>
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        <recommendedName>
            <fullName evidence="5">SIAH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8W9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0223200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223200"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF165">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="5">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="27662" checksum="EEEBA66AF8AB51BE" modified="2022-05-25" version="1">MCAVGHVICSSCHSNLPNKDKCHTCSHTGGYHRCNALDKILESFHVPCANAAYGCTTKTHYHRVEEHHKSCPHAPCFCPEPGCGFNGGSTIKLLDHLTSGHMWPSTELEYNVKLTLQAKAGAHVLHSRDGSPLFLVKFTPVPPYGIAASALCVDPDAATATEKGRKFRCHLGSSNVDMDWQQSSCFQVRSTTLSNGWPPAEDGGSLYVAPSTESITVSITKIMRDMRGNEIQLTPIRMRQSTLRQCFQSRGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B972</accession>
    <name>A0A8I7B972_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B972"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0235890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235890"/>
    </dbReference>
    <dbReference type="OMA" id="VLKEHSC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035323118" description="Knottin scorpion toxin-like domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8173" checksum="F35BB4A1CB4967BB" modified="2022-05-25" version="1">MGARNAIMCFVLILVLFPDNGSAGCWVSANGGPLCTGFLCKATCLIAEKMFNCVLKEHSCDGPPLNSECYCYLCDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B976</accession>
    <name>A0A8I7B976_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123439479</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B976"/>
    <dbReference type="SMR" id="A0A8I7B976"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0196050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0196050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0196050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000062">
        <property type="entry name" value="IQ-domain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF95">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="486"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="59649" checksum="788ADFDA7922D9DA" modified="2022-05-25" version="1">MHWQHCMRVLRAVPFPPFRISNPTRPFPQRQANALLLSATATPLPLQSPPHRALSEAHAHAVAHTCLLRPASFALLLLLAVSFLETLSFLLSGGRRSGAVLGVEEREMGGSGKWVKSLIGLKKPDREDCIKSKLLVPSVLGVGGKGRKWRLWRTSSGDHGSLWRGSRGGSQRSAASEASDDASSLAAAAADMFTAALATVARAPAKDFMAVRQEWAAIRIQTAFRGFLARRALRALKGLVRLQAIVRGRQVRKQAAVTLRCMQALVRVQARIRARRVRMSTEGQAVQKLIQARRTKLDILREAEEGWCDSQGTLEEVRVKLQKRQEGAIKRERAIAYVYQGVAKCNQPKGSNGRSNQSGLLLKHQHCDKNNGSWSWLERWMAARPWENRLMEEHNQTTTSSPDLVPSSKDCEDAFGVLADFSEPNSVKVRKNNVSKRISAKPPGATHPHRFKAQSISSLSTDLHNDESSASSSSCFASTPLSFSTLLTPEKTDGSGGVRSRPNYMSLTESIKAKRKPCGAQRTGASKQLDDRKAPGADLKAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAA8</accession>
    <name>A0A8I7BAA8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAA8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0287380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF28">
        <property type="entry name" value="OS02G0603500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19738" checksum="09B9E1E4606A5E2F" modified="2022-05-25" version="1">MDDDQTFLETIDFGYTQTQPDSPIGEQATPSTQHRSAITQKGKSNKGKNWSSDEDKVLIAAWANTSLDIVGTDQNRDVYWARISEYHNRHKESSWPERNDNAINCRYTLINKETSKFCGCLQQILNRHESGRPIEEKTNDAHILFKEMDLKKKKPFTLMHCYTEFSKHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BBF5</accession>
    <name>A0A8I7BBF5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123450933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044983893.1">
        <property type="nucleotide sequence ID" value="XM_045127958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BBF5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332050"/>
    </dbReference>
    <dbReference type="GeneID" id="123450933"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0276660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0276660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0276660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450933"/>
    <dbReference type="OrthoDB" id="2017354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36393">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36393:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="219" mass="23212" checksum="351B8DE7E20E57F7" modified="2022-05-25" version="1">MSLAPPPLLRLRASRLSAAGPSRPLSGCLATGRGPGSGRAYCLFSGAGAGRRKQEEARRGLESALDQNKAGFRTWGADTDTRRRRERHGGPAPAASGGGKGRSGGGGGGGWFRWFSSGGFWDAAKQTVLTILAIIAAVFLIANFNVLLAAAVYPLLVVLRQIRRAITFAAYCISRAMSAPTSRPKPTPVDRGEVVAPAPVKERARMSARERVVAKWRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BD71</accession>
    <name>A0A8I7BD71_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BD71"/>
    <dbReference type="SMR" id="A0A8I7BD71"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0649600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0649600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0649600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0649600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0649600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0649600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="45"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0649600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25150" checksum="E62562DF16C4D9BB" modified="2022-05-25" version="1">MPTTFHEVVAHGTTKLRVVYTNLSTEVPFFLQQLKERWFNHAPDHEKFFGLDLEYTADQRGVAVIQICFASHVLIFQWARSDKHCPGLMEFLRSGVTFASVDIRNDKLKMRHSFDIEIPAGCLVDLQTIFRLRHDRTSMAHMAVALIDESYGDMKTSFPKYHHKLWEKGPLDDINIQYAAKDAYVSYELYRKIRVVNYGQRHLEELGHSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDI4</accession>
    <name>A0A8I7BDI4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0546560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0546560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0546560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0658630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF40">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28711" checksum="C52099B8BFB0283F" modified="2022-05-25" version="1">MDALEARYRQFVLLASALGDDEEVVIPAALLASIEACCGFLPSEVSIQVACPPHDLWLFFSTEEKCMLVLQSSMKFKCCRRWIQFHRWVREIRATPAGLEYKTKLSFEGLPDQAWSMQSVKGVLKDLGGDLIEMIPPKNRRELEVTAWLRDPSKVAKLLDVEIPEPKLALCTDPPPESLDEFVAREEAPLYGPSSPKKKKTLVYPVICHLKEVVDRGPLLAEDLPDEWLPNEGEDLTQRHKFSIVLGKIDDNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BE46</accession>
    <name>A0A8I7BE46_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Morc S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123407719</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MORC ATPase protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956849.1">
        <property type="nucleotide sequence ID" value="XM_045100914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BE46"/>
    <dbReference type="SMR" id="A0A8I7BE46"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677390"/>
    </dbReference>
    <dbReference type="GeneID" id="123407719"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0677390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031349">
        <property type="term" value="P:positive regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045261">
        <property type="entry name" value="MORC_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041006">
        <property type="entry name" value="Morc_S5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23336:SF65">
        <property type="entry name" value="PROTEIN MICRORCHIDIA 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23336">
        <property type="entry name" value="ZINC FINGER CW-TYPE COILED-COIL DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17942">
        <property type="entry name" value="Morc6_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="Morc S5" evidence="12">
        <location>
            <begin position="751"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1036"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1104" mass="121176" checksum="EC294A38B533DB7F" modified="2022-05-25" version="1">MTLLDWIELSDEEATAEEEFPPQNVKDEFPPTKVKEEFPPTKVKEEFPPTIVKEEQGAHVHCHAAQLEVEPVDLTTEEDDVEEDRNAHGKRDAAQWAKTSHSQQFVAPHGQGEAAWTVVQRQEFLAAAKGVEEFLVTGDVAQETMQSGNQDFTAAVDCAEEATHSENAAEAGPSSFTEQGVVAGDCDEEAMQPGKQDFAAAVDCAEETMQYENAAEAGPSSFTEQGVVAGDCGEEAMQPGKQDFAAAVDCAEEAMQSENAAEAGTSSFTEQGVVAGDCNAEAMQPGNQNFAAAIDCAEEAMQSENAAKTGPSSFTEQGVVAGYCSEEAMQYGNQDFVAAVDCAEEAMQSENAAVAGPLSFTGQGFAAGVCGEEAMQSGKQDFIAAVDCSEEAMQSENAVEAAPSSFTDGVAAGDFAEEAMRYNHEDKAAECSPMTGQAGASSLSLTEQDATTSSLRTVQQSHKRDALLGSPPISTAAPFPRQFWKAGEYKVAARASINSGQNCLRINPKFLHSNATSHKWVFGAIAELLDNAVDEVQNGATYVKIDKIKHSPVGEYSLMIQDDGGGMSPEYIRHCLSFGFSNKCTNSSIGQYGNGFKTSTMRLGADAIIFSCNKANRLTRSVGLLSYTFLKGTGCDDILVPVVDYEFDPSSCNFKRIMDRGEQHFFSNLSTLLRWSPFSTEDDLLNQFEDMGCHGTKIVVFNLWLNDANEMELDFTTDGEDIMMSGAPKIPEERAKVKRLNHMHIANRFRYSLRVYASILYLRLPEHFEVILCGRTVEPHHIVKDLIYRECIKYRPQVGVSVQVDVVTSIGFLKGAPHLDIYGFNIYHRNRLILPFWAAGSERGRGRGIAGVLEANFIRPTHDKQDFEKTELFQRLETRLKDMTMEYWKHHAHLIGYRQITKSPPAYYASIAATHDNTPVAQAAATIYVRNSRAKASGVLDSHFNGGNSLNPLHVGALRDQMDYGICSLARRNMRTSLYMPSTPQQTEPCKRRNSGSMIETRAQKRHNTNGNADHLGSVNVVEFGDERSRVLICQKMVLKAECSELEAAAQNLQSKADRLRGELREWQRRQRSLTDELEFYDGLSAIRSRIPRASTSSMGVGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFU9</accession>
    <name>A0A8I7BFU9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="6">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Q-enzyme</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFU9"/>
    <dbReference type="SMR" id="A0A8I7BFU9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0751660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751660"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00152"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005983">
        <property type="term" value="P:starch catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000250">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000003">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF2">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="14">
        <location>
            <begin position="260"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="789"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="794"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="825" mass="93205" checksum="16188AA0F7A943F2" modified="2022-05-25" version="1">MPLLCLTSSSPSLRPAADRPGPGISGGGNVRLSVVPAPSSLRLSWPRKAKSKLSLPVSAPRDFTMATAQDGIDDLPIYDLDPKFAGFKDHFSYRMKKYRDQKHLIDEHEGGLEEFSKGYLKFGINTENDATVYREWAPAAKEAQVIGDFNNWNGSGHRMTKDNFGVWSIRISHVNGKPAIPHNSKVKFRFHRGDGVWVDRIPAWIRYAIADASKFGAPYDGVHWDPPTSERYVFKHPRPQKPDAPRIYEAHVGMSGEKPEVSTYREFADNVLPRVKANNYNTVQLMAIKEHSYYASFGYHVTNFFAASSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASSNMTDGLNGYDVGQNTQESYFHTGERGYHKLWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYNHHGINMSFSGDYKEYFGLDTDVDAVVYMMLANHLMHKLLPEATVVAEDVSGMPVLCRSVDEGGVGFDYRLAMAIPDRWIDYLKNKDDLEWSMSGIAHTLTNRRYTEKCIAYAESHDQSIVGDKTMAFLLMDKEMYTGMSDLQPASPTIDRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWSLVDIDHLRYKYMNAFDQAMNALDDKFSFLSSSKQIVSDMNEEKKVIVFERGDLVFVFNFHPNKTYDGYKVGCDLPGKYKVALDSDALMFGGHGRVAHDSDHFTSPEGIPGVPETNFNNRPNSFKILSPPRTCVAYYRVEEKAEKPKDGGAAFWGKTAPGYIDVEATGVKDATDGEATSGSEKVSTGDGSVKRGINFVFRSPDKDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGB5</accession>
    <name>A0A8I7BGB5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953745.1">
        <property type="nucleotide sequence ID" value="XM_045097810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGB5"/>
    <dbReference type="SMR" id="A0A8I7BGB5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547720"/>
    </dbReference>
    <dbReference type="GeneID" id="123403899"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0455210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0455210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0455210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0547720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403899"/>
    <dbReference type="OrthoDB" id="605137at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF317">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="13"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="2">
        <location>
            <begin position="102"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42036" checksum="88D4E967623635CC" modified="2022-05-25" version="1">MSPAAPAPLDDENLLSEILLRLPALPSTLPRVSLVCKRWRHLVSDIGFFRRFREHHRHSPPLLGCFVEGDRCVPYTPIMHSPDRIPAERFSLQLNETCHSWVLGCRHGLVLISPDSRKRVLVWDPVTGEQHHIAFPPGFVGAANSVHGAVLRPAEARHFQVILVWVGGEDKTNMPLLACVYSSEKGVWGSLISPLIQFEGYFKGLSSLLVGNSLYWCLGGRSDAILEFDLERQTLAVIQVPACLSEEDLLQFAAIRAEGGGLGLLSILGFTAQLWKRKTDSHGVASWAMGRTIELDKLLSMNPEKERVRPLIVGLAEYNNVVLLTTGGDGIIMLQLESLQFKKFYETTNSFYHHQFESVYSAGRSIGGGHDGAELLHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BHS2</accession>
    <name>A0A8I7BHS2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123404331</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044954189.1">
        <property type="nucleotide sequence ID" value="XM_045098254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BHS2"/>
    <dbReference type="SMR" id="A0A8I7BHS2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627980"/>
    </dbReference>
    <dbReference type="GeneID" id="123404331"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0521260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0521260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0521260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404331"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052486">
        <property type="entry name" value="PHO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48477">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48477:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="719"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="2"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="609"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="90317" checksum="4193AB05A7E76C3C" modified="2022-05-25" version="1">MVKFSREYEASIIPEWKGAFVDYKCLKKLVKKIKVARRDADDDSSAGGSSPETAALAAGVESVGYGAGFSMLDPVRALAARFGPRVQASTDDEESGDSRELVRSTDKHEREFLEKADEELEKVNTFYASQETELLGRGEALIDQLRILADVKRILADHAATRRARGSLLGRSRSMPPVAPPSPALSNSGRYLLSGLATPQSMSDGSVEQQQAQMTEGAAVADEVMAALERNGVSFVGLPGKKDAKKDGGNRRLQLPSTVRIDIPASNPGRAALKVWEELVNVLRKDGADPAAAFVHRKKVQHAEKNIRDAFMALYRGLELLKKFSSLNVKAFTKILKKFVKVSEQQRATDLFSQKVKRSSFSTSDKVLQLSDEVESLFLKHFAGNDRMVAMKYLNPQQPKNTHMITFLVGLFTGTFVSLFIIYAILAHVSGIFASAGNTAYMEVVYHVFSMFALISLHCFLYGCNLFMWKSTRINQNFIFDFAPNTALTHRDAFLMSASIMCTVVAALVINLFLRNAGASYANAVPGGLIVLSAGLLFCPFNVFYRSTRYCFMRIMRNIMFSPFYKVLMADFFMADQLTSQIPLLRHMEFAACYFMAGSFRANPYETCTNSQQYKHLAYVISFLPYYWRAMQCLRRYIEEHDINQLANAGKYVSAMVAAAVRFKYNVTPTPFWMWMVLISSSGATVYQLYWDFVKDWGFFTPKSKNLWLRDDLILKNKFTYYLSMMLNLVLRLAWAQSVMKIRINKNETRLLDFSLASLEIIRRGHWNFYRLENEHLNNVGKFRAVKTVPLPFRELETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIE3</accession>
    <name>A0A8I7BIE3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor GAMYB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">OsGAMyb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123410587</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044959468.1">
        <property type="nucleotide sequence ID" value="XM_045103533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIE3"/>
    <dbReference type="SMR" id="A0A8I7BIE3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656270"/>
    </dbReference>
    <dbReference type="GeneID" id="123410587"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0544570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0544570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0544570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0656270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410587"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000119">
        <property type="entry name" value="Transcription factor GAMYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47995:SF1">
        <property type="entry name" value="OS03G0578900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47995">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0287">Flowering</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="15">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="14">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="15">
        <location>
            <begin position="90"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="14">
        <location>
            <begin position="90"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="241"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="427" mass="46252" checksum="0DDE41EEA6443123" modified="2022-05-25" version="1">MDAASLEEEKMAAAAAAADNLDEEAEQGGEGEEEAMPVVLKKGPWTAAEDALLVNHVRQHGEGNWNAVQRITGLLRCGKSCRLRWTNHLRPNLKKGAFSPDEELLIAQLHAQLGNKWARMATHLPGRTDNEIKNYWNTRAKRRQRAGLPMYPPEVQFQLAITKRCRYDDFTPPQQSAGGNVLDATDARYTSARPPPLDLAGQLAMANRPVQFLAQTPFSAPSSPWVKPSFARNARYFQFPHSSPVSPTTPVHPVTPELSLGHGQHGGERSRFTPLSPSPGAKAELPSGQLLPASPAAATPTSGEDQQNLEAMLQELHDAIKIEPPKHVSDHAEQDGGSGGNKPEGELKDDDIGTLLDMIIPATFPMTEPAPPGVAPDNSGGSISQNGSNDHDHHNVDLSVDHIPIISSEQDWVLDGACQWNNMSGIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-01-09" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>A0S6X4</accession>
    <name>A0S6X4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FT-like protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FT2</name>
        <name evidence="4" type="synonym">LOC123443129</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2007" name="Genetics" volume="176" first="599" last="609">
            <title>The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare).</title>
            <authorList>
                <person name="Faure S."/>
                <person name="Higgins J."/>
                <person name="Turner A."/>
                <person name="Laurie D.A."/>
            </authorList>
            <dbReference type="PubMed" id="17339225"/>
            <dbReference type="DOI" id="10.1534/genetics.106.069500"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ297407">
        <property type="protein sequence ID" value="ABB99414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK373041">
        <property type="protein sequence ID" value="BAK04238.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0S6X4"/>
    <dbReference type="SMR" id="A0S6X4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0203250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0203250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0203250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0244930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244930"/>
    </dbReference>
    <dbReference type="OMA" id="MQPRVHI"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0S6X4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000001">
        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF113">
        <property type="entry name" value="PROTEIN FLOWERING LOCUS T 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABB99414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK04238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="178" mass="19678" checksum="CC4B8D0738029F43" modified="2007-01-09" version="1">MVGSSMQRGDPLVVGRVIGDVVDPFVRRVALRVGYASRDVANGCELRPSAIADQPRVEVGGPDMRTFYTLVMVDPDAPSPSDPSLREYLHWLVTDIPATTGVSFGTEVVCYEGPRPVLGIHRLVFLLFQQLGRQTVYAPGWRQNFSTRDFAELYNLGLPVAAVYFNCQRETGTGGRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQA0</accession>
    <name>F2CQA0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123396814</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK353802">
        <property type="protein sequence ID" value="BAJ85021.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044947560.1">
        <property type="nucleotide sequence ID" value="XM_045091625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQA0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0477510.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0477510.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0477510"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0477510.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0477510.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0477510"/>
    </dbReference>
    <dbReference type="GeneID" id="123396814"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0395560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0395560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0395560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0477510.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0477510.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0477510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0477510.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0477510.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0477510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396814"/>
    <dbReference type="HOGENOM" id="CLU_098173_0_0_1"/>
    <dbReference type="OrthoDB" id="686813at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34464">
        <property type="entry name" value="OS09G0376300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34464:SF3">
        <property type="entry name" value="OS09G0376300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ85021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0477510.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="175" mass="19093" checksum="1732F2ACC333E3B6" modified="2011-05-31" version="1">MAMGLGRFTHWLWPGNAARVGNHELPGTALTGASFPEFPSGFREADALAFSTAAAGGRRARQRRVKNQRRSRGEPRIDREYDMVIVPSDGGGCLSGSESDDSDWSIGWLEPQAPEMQTDGDQETSFAVLVPCYRHGRAEQPAMPQARFLGAGPLADRGLSDGKNFVEEWLSSLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQC6</accession>
    <name>F2CQC6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123406235</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot</tissue>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK353828">
        <property type="protein sequence ID" value="BAJ85047.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK363503">
        <property type="protein sequence ID" value="BAJ94706.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955655.1">
        <property type="nucleotide sequence ID" value="XM_045099720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQC6"/>
    <dbReference type="SMR" id="F2CQC6"/>
    <dbReference type="STRING" id="112509.F2CQC6"/>
    <dbReference type="PaxDb" id="4513-MLOC_8289.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596460"/>
    </dbReference>
    <dbReference type="GeneID" id="123406235"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0494280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0494280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0494280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0596460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0596460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0596460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406235"/>
    <dbReference type="eggNOG" id="KOG0133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CQC6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
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        <property type="entry name" value="SI:CH1073-390K14.1"/>
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        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
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    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="9">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
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        <location>
            <position position="232"/>
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        <ligand>
            <name>FAD</name>
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            <begin position="244"/>
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            <name>FAD</name>
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            <name>FAD</name>
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            <position position="321"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001932"/>
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            <dbReference type="ARBA" id="ARBA00005862"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
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    <sequence length="656" mass="73991" checksum="E0DC5458FA67818F" modified="2011-05-31" version="1">MPGSERTVVWFRKDLRIDDNPALAAAARDGAVLPVFIWCPAEEGRFYPGRCSRWWLKESLAHLAKSLEALGCPLVLIRAQTTLDALLQCVHSIRATRVVYNHLYDPISLVRDDKIKNELLGLGISMQSFNGDLLYEPWEIYDENGLAFTTFNMYWEKCMELPIEISPSLAPWRLVPVSGIENLCTSSIDNLGLESSKDEESSNALLSRAWSPGWRNAEKTLEDFVCHGLLDYSKDGMKVAGTTTSLLSPYLHYGEVSVRKIYQLVRMQQIKCGNEGNSEAGESVNLFLRSIGLREYSRYLCFNFPFTHERSLLGNLKHYPWRADEDRFKSWRQGMTGYPLVDAGMRELWATGWTHNRIRVIVSSFAVKFLLIPWTWGMKYFWDVLLDADIESDILGWQYISGCLPDGHELGRLDNPEVQGQKYDPDGEYVRTWIPELARMPGEWIHHPWDAPSSILEVAGVELGFNYPMPIVELHMARECLDDAISTMWQLDTAEKLAELDGEVVEDNLSHIKSFDLPKVVLKELSPSAPHCDQKVPTDDGRNLELQPKELTGLNKQTICVDVIKASKMEDTGSIVNSPISRKRSSSGSVFDVPSCSSSVEVRSQNQRPGGYFVGSSKYILQKAERNCFDKAEDDDSAHSGTNTSRASKRPAASQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2CUT6</accession>
    <name>F2CUT6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123404226</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK355388">
        <property type="protein sequence ID" value="BAJ86607.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK355803">
        <property type="protein sequence ID" value="BAJ87021.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044954074.1">
        <property type="nucleotide sequence ID" value="XM_045098139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CUT6"/>
    <dbReference type="PaxDb" id="4513-MLOC_73543.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0622550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0622550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0622550"/>
    </dbReference>
    <dbReference type="GeneID" id="123404226"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0516530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0516530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0516530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0622550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0622550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0622550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404226"/>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CUT6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF119">
        <property type="entry name" value="OS02G0789100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ion_trans"/>
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        <property type="entry name" value="cNMP"/>
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        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
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    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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            <end position="97"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="367"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F2CX85</accession>
    <name>F2CX85_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC123402450</name>
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    <organism evidence="2">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0602530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="128" mass="13451" checksum="6C9E1EB8CDDD9AFB" modified="2011-05-31" version="1">MELEAAPQLPRRDARKLVRCPRLQLDAKTVTAVEQSTGTPVADAAATSPGGAMRVKILLSKQQLKQVAAAVAAGGAFGLPPALEQLVSAIKRQHAKKQAAPTNAAAARRPGRWSPALHSIPEDISYLS</sequence>
</entry>
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    <accession>F2CY80</accession>
    <name>F2CY80_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
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    <organism evidence="3">
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAJ87801.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045095482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CY80"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598170"/>
    </dbReference>
    <dbReference type="GeneID" id="123401642"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0598170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401642"/>
    <dbReference type="OrthoDB" id="1883054at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33427:SF1">
        <property type="entry name" value="F6A14.21 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33427">
        <property type="entry name" value="HNH ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="2">
        <location>
            <begin position="59"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ87801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="175" mass="19743" checksum="231184F6611B00C0" modified="2011-05-31" version="1">MAPSTSEREHNSEASSSGVGSKSKEKERPRSFDEKTRTACWRKAAVLTGRHPERWRQDAAGNVVCRRFWSCHGCLCYEYDHIVPFSKGGESTVENCQILQTRVNRSKSDKAWVEKAEMQGFSCDIKFTDKELDVIEMAVYGDVIRPGKQCRCKTVAEMLGQVKSKNQMAACNLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYI6</accession>
    <name>F2CYI6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123427036</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M76 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AK356691">
        <property type="protein sequence ID" value="BAJ87907.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966903.1">
        <property type="nucleotide sequence ID" value="XM_045110968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYI6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176290"/>
    </dbReference>
    <dbReference type="GeneID" id="123427036"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0145780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0145780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0145780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0176290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0176290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0176290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427036"/>
    <dbReference type="OrthoDB" id="285308at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ87907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0176290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="22402" checksum="CF0965BF4C508CC3" modified="2011-05-31" version="1">MAAAAGDGGSVPPKSPTAEAPPASQPRLTPNTMPWERCVAGVQSALKDPLARFLREQIEKAGCTVWPTLIRAAICTASGGYTSGKGIEVCCNHMRKQDEITQVIIHELVHAYDDCVVKNINWNNCAHHACSEIRANHLSGNCHYKRELMKGFLKIKGHEPECVKRRALLSVQNNPSCRGKAAKEAVENVWDTCYNDPRPFDKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2D405</accession>
    <name>F2D405_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123411789</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK358613">
        <property type="protein sequence ID" value="BAJ89826.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960687.1">
        <property type="nucleotide sequence ID" value="XM_045104752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D405"/>
    <dbReference type="SMR" id="F2D405"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0745140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0745140"/>
    </dbReference>
    <dbReference type="GeneID" id="123411789"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0617930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0617930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0617930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0745140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0745140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0745140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411789"/>
    <dbReference type="HOGENOM" id="CLU_023075_4_0_1"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF115">
        <property type="entry name" value="HEPTAHELICAL TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ89826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="369" mass="41290" checksum="F9F14627FA27E353" modified="2011-05-31" version="1">MDQESSMGGGQLHEQEAAASAAMAKKRRGKQRRSSEGRKRRQQQQQEYRLVSYEELPEYMKENEFILNHYRSEWPLLHAFLSVFSWHNETINIWTHLLGFFLFLGLTLWHLAQYFPQVAHLIGHLSWPISKVAENVSSNIGDVLSGAASFMQASPGLAMAAAAGVGPTTRWPFFVFLAGAMFCLMSSCACHLLSCHSHRLNLFLIRLDYTGIAVMIVVSFFPPIYYIFQCEPRWQVAYLSAISLAGAATVYALMSPRLSDAKYRAHRALLFVGMGLSGVVPAVHAAAVNWHEPRRNVTLAYEGAMAASYLVGTAFYLTRVPERWRPGMFDLCGQSHQIFHLLVIAGALAHYGAAIVFLRVRDEMGCPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDX8</accession>
    <name>F2DDX8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endoglucanase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123400106</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
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        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045094516.1"/>
    </dbReference>
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    <dbReference type="PaxDb" id="4513-MLOC_44777.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499440"/>
    </dbReference>
    <dbReference type="GeneID" id="123400106"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0414480"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499440.1"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="10257085at2759"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DDX8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
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        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_9"/>
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    </dbReference>
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        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH9_2"/>
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    </dbReference>
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    <keyword evidence="3 8 9" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="531"/>
        </location>
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            <begin position="61"/>
            <end position="517"/>
        </location>
    </feature>
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            <begin position="33"/>
            <end position="55"/>
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    </feature>
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            <end position="54"/>
        </location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFB4</accession>
    <name>F2DFB4_HORVV</name>
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        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
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    <organism evidence="4">
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DFB4"/>
    <dbReference type="PaxDb" id="4513-MLOC_15510.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185510.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0153610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0153610.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0185510.1">
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="GATA26/27"/>
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        <property type="entry name" value="Znf_GATA"/>
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        <property type="entry name" value="Znf_NHR/GATA"/>
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        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46855">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ93785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="441" mass="48234" checksum="18EE0D148EFECF80" modified="2011-05-31" version="1">MGKEGPCRHCGVTSTPLWRNGPLNKPVLCNACGSRWRTKGSLENYTPMHSRDDIDAEQPRVSKLKPPTLRLKEQRQVKKKPSHSIRENGAFSDQNFWKMGDADPSRSSSGSALSYSESCAPYGSADVSEMTGSAQSHAWESLVPSKKRSYVTRTKSSSVDMLVKDLHCIMHEEQLSYLSGSSEEDLIYHNATPVGSFEIGYGSMLLRSSNSKSAEEDSEANSVPADNKSYLTSESYSGTASFVVHSESKGASNSNAAPEKPKWFPVQTHENVKRGKLHYSKQHTLENVGSALVSVALEGEDTKETGGNENTSALKDLTKSNMKPLKRPHESQLQSCPEGTMRIAKKVCKSVTMAPQFKGSFLPKSGGAPFNLLMLPPDKISMLAPPQYMDNSDQDLLLEVPLNARQPEAELLYQPFQLSSVARSSTSADVVADGEGRLKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F2DGG0</accession>
    <name>F2DGG0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123443452</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="EMBL" id="AK362977">
        <property type="protein sequence ID" value="BAJ94181.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975761.1">
        <property type="nucleotide sequence ID" value="XM_045119826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DGG0"/>
    <dbReference type="SMR" id="F2DGG0"/>
    <dbReference type="FunCoup" id="F2DGG0">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2DGG0"/>
    <dbReference type="PaxDb" id="4513-MLOC_56594.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255020"/>
    </dbReference>
    <dbReference type="GeneID" id="123443452"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0211760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0211760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0211760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0255020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443452"/>
    <dbReference type="eggNOG" id="ENOG502QR5A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_1_1"/>
    <dbReference type="OMA" id="MAISMSH"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DGG0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF54">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019606" description="Peroxidase" evidence="18">
        <location>
            <begin position="25"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="29"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="39"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="72"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="125"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BAJ94181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="333" mass="35981" checksum="D05683069FBCE981" modified="2011-05-31" version="1">MATSMSCLLVLCLVCPFLVGVVRANPWYGLFPQFYDHSCPKAKEIVQSIVAQAVAQETRMAASLVRLHFHDCFVKGCDASVLLDNSSSIVSEKGSNPNLNSLRGFEVVDQIKVALETACPGTVSCADILALAARDSTVLVGGPYWDVPLGRRDSLGASIQGSNNDIPAPNNTLPTIITKFKRLGLNVVDVVALSGGHTIGLSRCTSFRQRLYNQSGNGLADSTLDVSFAAQLRQGCPRSGGDNNLFPLDVVSSTKFDNFYFKNILAGRGLLSSDEVLLTKSAETAALVKAYANDVHLFFQHFAQSMVNMGNIMPLTGSQGEIRKDCRRLNNYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2DID9</accession>
    <name>F2DID9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426896</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363657">
        <property type="protein sequence ID" value="BAJ94860.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966734.1">
        <property type="nucleotide sequence ID" value="XM_045110799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DID9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173040.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173040"/>
    </dbReference>
    <dbReference type="GeneID" id="123426896"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0143010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0143010.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0143010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0173040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0173040.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0173040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426896"/>
    <dbReference type="HOGENOM" id="CLU_019429_0_0_1"/>
    <dbReference type="OrthoDB" id="77038at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056866">
        <property type="entry name" value="Znf_WRKY19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31827">
        <property type="entry name" value="EMB|CAB89363.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31827:SF62">
        <property type="entry name" value="OS04G0458200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24906">
        <property type="entry name" value="Zf_WRKY19"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ94860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0173040.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="632" mass="65577" checksum="CD7922F3843CBC43" modified="2011-05-31" version="1">MDNNFVDIPNQPLMNDPFVLVAQSAPSYALNNLGKSTVRMDCLGSGMANCGHGHESTQNMNNITATDDGCRLVLGLGPTPDFYSTHYQPTGVYKSKESQTLSGQSFCFTDPGVLRLGLQTDGAETIQPLQTPNERVHSFPVVDEASTSAAVRSMGGYMPSLLFAPRSSSSAATEAQLQTRDSLNPTHYNSNNTQHIQQHPQLSPEPSATTETSFGVSSDVVTGATTSEQRSHSRHPKKCRFKGCSKGGRGSSGLCIAHGGGQRCHKPGCHKGAESSTAYCKAHGGGRRCEELGCTKSAEGKTDYCIAHGGGRRCEYPDCPKAARGKSGRCIKHGGGKRCAMEGCIRSAEGKAGLCISHGGGRRCQYPDCAKGAQGSTLYCKAHGGGKRCVFDGCSRGAEGSTPLCKAHGGGKRCMFEGGGVCPKSVHGGTEFCVAHGGGKRCAAPGCTKSARGRTDCCVKHGGGKRCRIDNCGKSAQGSTDFCKAHGGGKRCTWGSGCEKFARGKSGLCAAHGTLVARQQEHGMVKSGGSMIGPGLFSGIVASSTTAASSMTNEHSSSGISTASDSDGTVRSQSMIPPQLLVPRSMMPSSSSEPTVRGGKEAGCAVPEGRVHGGGLLSLLGGSFRNVNIDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F2DNB4</accession>
    <name>F2DNB4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Potassium channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123443974</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Potassium channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the potassium channel family. Plant (TC 1.A.1.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK365382">
        <property type="protein sequence ID" value="BAJ96585.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976493.1">
        <property type="nucleotide sequence ID" value="XM_045120558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DNB4"/>
    <dbReference type="SMR" id="F2DNB4"/>
    <dbReference type="FunCoup" id="F2DNB4">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2DNB4"/>
    <dbReference type="PaxDb" id="4513-MLOC_74879.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275790"/>
    </dbReference>
    <dbReference type="GeneID" id="123443974"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0228480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0228480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0228480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0275790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443974"/>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DNB4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000074">
        <property type="entry name" value="Potassium channel KAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000123">
        <property type="entry name" value="Potassium channel KAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045319">
        <property type="entry name" value="KAT/AKT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021789">
        <property type="entry name" value="KHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743:SF2">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11834">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51490">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 14" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4 14" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="7 14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="392"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="574"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="607"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="KHA" evidence="16">
        <location>
            <begin position="820"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ96585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="898" mass="100923" checksum="CAB1BBA7D18D0073" modified="2011-05-31" version="1">MSSRSGAARMRACGPWGEASGSGAVGDAHALEREMSRDGSHYSLSSGILPSLGARSNRRVKLRPFIISPYDRRYRLWETFLIVLVVYSAWISPFEFGFIRIPTGGLAATDNAVNAIFAIDIILTFFVAYLDRLTYLLEDDPKRIAWRYATSWLVLDVASTIPSEIARRMLPSKLRSYGFFNMLRLWRLRRVSSLFARLEKDRHFNYFWVRCAKLICVTLFAVHCAACFYYLLADRYPNPQETWIGNTMPDFHSKGLWIRYVTSVYWSITTLTTVGYGDYHAENIREMIFNIFYMFFNLGLTAYLIGNMTNLVVHGTSRTRKYRDTIQAATSFALRNQLPPRLQDQMISHLSLKFRTDSEGLQQQETLDALPKAIRSSISQYLFLNLVQNIYLFQGVSNDLIFQLVSEMKAEYFPPREDVILQNEAPTDFYILVSGSVELVEVPNGAEHGAEQVVGVAKSGDVIGEIGVLCYRPQLFTVRTRSLCQLLRMNRTAFLSIVQSNVGDGTIIMNNLIQLLKEQKDGVMVGVLKEIESMLARGRLDLPITLGFAVTRGDDHLLHQLLKRNLDPNESDQDGRTALHIAASKGNEQCVKLLLDYGADPNARDSEGKVPLWEAVYAKHDTVVQLLIKGGAELSAGDTSLYACTAVEQNDIELLKQILKHVIDVNRPSKDGNIPLHRAVCDGNVEMVELLLRHGADIDKQDSNGWTPRALAEQQGHEEIQNLFRSVVAPRKYTSNGRVTPMLLGRFSSDPSMQKVIREDAEQQPSKVLPQRRKVSFHNSLFGVISSSHPRRETDHLLSRGLAATGGPSYPQAHHRPLIRVTIGCPEKGNTAGKLVILPGSMKELLQLGAKKFDMMPTKVLTIEGAEVDEVELIRDGDHLVLASDDWVPDDTQIRGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2DUU8</accession>
    <name>F2DUU8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395847</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367666">
        <property type="protein sequence ID" value="BAJ98869.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946817.1">
        <property type="nucleotide sequence ID" value="XM_045090882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DUU8"/>
    <dbReference type="SMR" id="F2DUU8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491140"/>
    </dbReference>
    <dbReference type="GeneID" id="123395847"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0407090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0407090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0407090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0491140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395847"/>
    <dbReference type="HOGENOM" id="CLU_007383_9_0_1"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000219">
        <property type="entry name" value="Cinnamoyl-CoA reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF660">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ98869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="344" mass="37644" checksum="667DEAC59D4676CA" modified="2011-05-31" version="1">MAGRPEGGKGETVCVTGAGGYIASWLVKLLLSRGYTVRGTVRDLGEKKTGHLRSLENASENLKLIKADLLDNDAMAAAIEGCQGVFHVASPVPTGDVTDPEVEVLGPAATGTRNVLEAASAAKVRRLVVVSSIVAVDINPKDWPADKIKDETCWSDREFCRNNEDWYSVSKITAEEAALEYRQRTGLDVVTPNPAVVFGPLLQPTVNASSQFLIYFLKGGPGRMRDKLWHIVDVRDTADALLLLYEAPEAVGRHICAPHVITARDLRDMLKNMYPDYPLVSKESICDMDHPAPMTTDKLKKLGWSCRSLEETITDTVKFCQQAGFLDDVDGAGPCRFPPIYNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2E6R4</accession>
    <name>F2E6R4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401643</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FLZ family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371838">
        <property type="protein sequence ID" value="BAK03036.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951419.1">
        <property type="nucleotide sequence ID" value="XM_045095484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E6R4"/>
    <dbReference type="STRING" id="112509.F2E6R4"/>
    <dbReference type="PaxDb" id="4513-MLOC_21885.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0590450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0590450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0590450"/>
    </dbReference>
    <dbReference type="GeneID" id="123401643"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0489450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0489450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0489450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0590450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0590450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0590450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401643"/>
    <dbReference type="eggNOG" id="ENOG502S3GA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143767_0_0_1"/>
    <dbReference type="OMA" id="GNHNIFS"/>
    <dbReference type="OrthoDB" id="1926521at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E6R4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33059:SF84">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33059">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FLZ-type" evidence="6">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="5">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK03036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0590450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="121" mass="12841" checksum="07597DD3B4E15764" modified="2011-05-31" version="1">MAGLSVLLETHEGNSTTPKIISKATLHSPKISSSSPWSPAARPAASTGSFLQRCLLCHKDLAENNDIYMYRGDKAFCSVECRCRQIFIDEDAGSSHCAKGASTAAVRSGRRATGGGVSFAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2EF72</accession>
    <name>F2EF72_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123413749</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374798">
        <property type="protein sequence ID" value="BAK05994.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962552.1">
        <property type="nucleotide sequence ID" value="XM_045106617.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962553.1">
        <property type="nucleotide sequence ID" value="XM_045106618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EF72"/>
    <dbReference type="SMR" id="F2EF72"/>
    <dbReference type="STRING" id="112509.F2EF72"/>
    <dbReference type="PaxDb" id="4513-MLOC_68237.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070350"/>
    </dbReference>
    <dbReference type="GeneID" id="123413749"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0070350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413749"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022744_2_0_1"/>
    <dbReference type="OMA" id="GPLHVVP"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF478">
        <property type="entry name" value="OS05G0485800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090942" evidence="2">
        <location>
            <begin position="32"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="42"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="171"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="388"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK05994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="560" mass="62195" checksum="FA3574843B001487" modified="2011-05-31" version="1">MATTMTMRRTAAGGVLLVAALLLLSTRVARAEDPYVFFEWHVTYGTKNIVGTPQKVILINGEFPGPRINCSSNNNIMVNVFNQLDQPLLFTWNGIQHRKNSWQDGVPGTNCPVAPGTNYTYHWQPKDQIGTFFYFPSIGMQRAVGGFGLISVVSRLLIPVPFDPPADDLQVVIGDWFTKDHAVMASLLDAGRSFGRPAGVLINGKGGKEATNPPMFTWEAAKTYRLRICNVGIKSSLNFRIQGHDMKLVEIDGSHTLQDSYDSLDVHVGQCLSVLVDADQKPADYLMVASTRFMADASSVSAVVRYAGSNTPPAPNVPEPPAGWAWSLNQWRSFRWNLTASAARPNPQGSYHYGQINITRTIKLLVTRGHIDGKLKYGFNGVSHVDADTPLKLAEYFNISDQVFKYNQMADTPPAVNGPMHVAPNVITAEFRTFIEVVFENPEKSMDSVHIDGYAFFAVGMGPGKWTPDQRKTYNLLDAVSRHTIQVYPRSWSAVMLTFDNAGMWNVRSNLWERHYLGEQLYMSVVSPARSLRDEYNMPENALRCGKVVGLPLPPSYLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFP2</accession>
    <name>F2EFP2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123406998</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK374968">
        <property type="protein sequence ID" value="BAK06164.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955955.1">
        <property type="nucleotide sequence ID" value="XM_045100020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFP2"/>
    <dbReference type="SMR" id="F2EFP2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0744180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0744180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0744180"/>
    </dbReference>
    <dbReference type="GeneID" id="123406998"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0617040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0617040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0617040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0744180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0744180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0744180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406998"/>
    <dbReference type="OrthoDB" id="650824at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34397">
        <property type="entry name" value="OS05G0237600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34397:SF19">
        <property type="entry name" value="TF-B3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK06164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0744180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="18309" checksum="8930BB22A9572159" modified="2011-05-31" version="1">MDSRSRIDAAEEARRRQVMDSLGVYDAEWALSKVLEQSDVQAGQNRLLLTKEAVHAGPIPKLFPELDELRDDGMNAENKVSVKVLDAEGREKNVSLRFLNSNKAYRIMGPDWRRLVEESRMRKGDRLDIYTCRRGDGERCLFVFMIRAVVPEIPAGATVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKI6</accession>
    <name>F2EKI6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123424684</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Seed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable thiol-disulfide oxidoreductase that may participate in various redox reactions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376663">
        <property type="protein sequence ID" value="BAK07858.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964283.1">
        <property type="nucleotide sequence ID" value="XM_045108348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKI6"/>
    <dbReference type="SMR" id="F2EKI6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0111290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0111290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0111290"/>
    </dbReference>
    <dbReference type="GeneID" id="123424684"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0091330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0091330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0091330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0111290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0111290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0111290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424684"/>
    <dbReference type="HOGENOM" id="CLU_055041_3_1_1"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000199">
        <property type="entry name" value="Thioredoxin-like 1-2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF34">
        <property type="entry name" value="THIOREDOXIN-LIKE 1-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="55"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK07858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0111290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="270" mass="28825" checksum="0DBBF940B6562880" modified="2011-05-31" version="1">MANALYGGGVAAPCGDLGAAAALAESLPMGGGYRARSSFPAGRVALAERPLPRSLQVAAAAGQMNGNLTIGKAMRWWEKGTQPNMREVESAQDLADSLLNAGDKLVVVDFFSPGCGGCRALHPKIAQFAERNPDVLFLQVNYEKHKSMCYSLHVHVLPFFRFYRGAQGRVSSFSCTNATIKKFKDALAKHSPDRCSLGPARGLEKAELLALAENRDLEFTYSEKPTLVPIAEAIRMEAASIGGPWLPLPPAATQPFPLGSENGSLIPSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKT3</accession>
    <name>F2EKT3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401661</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Seed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK376761">
        <property type="protein sequence ID" value="BAK07955.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951440.1">
        <property type="nucleotide sequence ID" value="XM_045095505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKT3"/>
    <dbReference type="SMR" id="F2EKT3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571280"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571300"/>
    </dbReference>
    <dbReference type="GeneID" id="123401661"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0473610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0473610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0473610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0473620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0473620.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0473620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0571280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0571300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401387"/>
    <dbReference type="KEGG" id="hvg:123401661"/>
    <dbReference type="OrthoDB" id="681725at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35361:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35361">
        <property type="entry name" value="OS08G0443700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK07955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571280.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="21466" checksum="15CF7D9A8FA9BACC" modified="2011-05-31" version="1">MTSQVAATLPPLLPTPAHCLLLAPPPAVYTARVELDSKKQQPGRASMSRSWIRDKVDLPGRASGSSSWITDKTLRRAETLDGGVERLGRVGTPREKWKRPASRAPSIDRCEKKLRPPTEMLTHSEASLIAGPVASVDWSEKKTTPLTDRSEQKPKPLAKMEATSEASFFAGPTFILSPDPSELPMPTFLYKQAQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKV5</accession>
    <name>F2EKV5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAK07977.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0006450.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.1HG0006450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0004650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0004650.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0004650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0006450.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.1HG0006450"/>
    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090980" evidence="2">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK07977.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="99" mass="10123" checksum="CE99EFB7F83AD5D6" modified="2011-05-31" version="1">MVSSMARGLTIAVFLLVFVAATRPCTHAVKLQATDAPPAAEGPAGISSQAEGPDGPAYVELVIKHPYPASSLNSRSGGLPVDPTPDGLPVDPTPEGQYK</sequence>
</entry>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the QWRF family.</text>
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    </dbReference>
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    <dbReference type="PaxDb" id="4513-MLOC_10572.3"/>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="entry name" value="AUGMIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS09G0422600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <begin position="1"/>
            <end position="107"/>
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    </feature>
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        <location>
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            <end position="255"/>
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        <location>
            <begin position="13"/>
            <end position="41"/>
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    </feature>
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            <end position="76"/>
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            <end position="350"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010016"/>
        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="63128" checksum="9CE1FBEC539CF36E" modified="2013-04-03" version="1">MDVHKSTVQKAALVEETRRPPLVPSEKHNASSLSRVRDVASRYKTGQGATATRRCTSPSLGRASTTNGTPVPNRAQSADRRRPSTPSTPSSKVSTPSTPTSRSITPVRDTVKEVNKSSRCIANERSPHGLWPAMRNLSPSYQLESAAALGNKRDKVVSSPSLDHIKGQASVPTERKRSPLRRKRITEQCENSRPSEDLPKRTTEQNRWPAMTSGRGPSNLMSNTDLSDKSSRPVPLANTSRGLSPRKISASEDAGKRLNQSLDDVARRLAIHAKRKGEQLDSVSDVYSQTTERSKYVSRPSRTTTLPVPVLHRSSSPNKVLSAASSASRAFQSPSRTRPSTPCRSQSAGSIQSGVIAPVVSYMLDPRKGKKNASQIENIHQLRLLHNRYLQLRLVNARSEDVLSFQKATTENTIYNVWRDTSNLRDGVNLRRIMVQRHRQELKLYGILQEQIAYLEQWPALEDENNLSFSRAAEALKASTLRLPVTSGARVDAVSLKNAVSSAVDVMQGLGSSVCSMLSKVEDRTYLVSELSVVAAQEKVMLDECRELLAMAAELEVQESSLRTHLMQVQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>M0W7Z4</accession>
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            <fullName evidence="3">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045104809.1"/>
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    <dbReference type="SMR" id="M0W7Z4"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0640610"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0640610.2"/>
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        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
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        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02252">
        <property type="entry name" value="DREG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46649">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46649:SF5">
        <property type="entry name" value="F14L17.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0640610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28752" checksum="D8B40FE36DD0C757" modified="2013-04-03" version="1">MPAAVVRCPLLLRRHPAAALRRSFSSSGMRSVRRAAGGGSQGRSPAYRGLLLDAGGTLLQLAQPVAETYATLGRPYGVMKSKEYIMQGFKRAFSAPWPKTLRYQGDGRPFWRIVVAEATDCTNSDYFEEVYQYYAHGDAWRLPDGAYRTLRDLKDAGVKLAVVSNFDTRLRKLLKDLNVSNMFDAIVVSSEVGYEKPAPEIFKIALEQIGVEASNAVHVGDDETADKAGANAIGLECWLWGEDVKEFSEIQRRIVAKGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0WAP6</accession>
    <name>M0WAP6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123428241</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968368.1">
        <property type="nucleotide sequence ID" value="XM_045112433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WAP6"/>
    <dbReference type="SMR" id="M0WAP6"/>
    <dbReference type="FunCoup" id="M0WAP6">
        <property type="interactions" value="1947"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WAP6"/>
    <dbReference type="PaxDb" id="4513-MLOC_4672.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207400"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207400.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207400.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207400"/>
    </dbReference>
    <dbReference type="GeneID" id="123428241"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0172390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0172390.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0172390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207400.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207400.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428241"/>
    <dbReference type="eggNOG" id="KOG3235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MSMQNAN"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WAP6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000037">
        <property type="entry name" value="N-alpha-acetyltransferase daf-31-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="155"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="195" mass="21800" checksum="C7D2D2383DA65886" modified="2013-04-03" version="1">MVCIRQATIDDLLAMQACNLMCLPENYQMKYYFYHMLSWPQLLFVAEDYGGRIVGYVLAKMEEDPSEPCHGHITSLAVLRSHRKLGLATKLMSAAQAAMDQVFGAEYVSLHVRRSNRAAFNLYTSTLGYQIHDIEAKYYADGEDAYDMRKMLRQPVPKKHHHHHHAGGGCCSHDALPAPAGSSPTTASPEKKADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0XUK9</accession>
    <name>M0XUK9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-arabinopyranose mutase</fullName>
            <ecNumber evidence="6">5.4.99.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123428052</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose</text>
            <dbReference type="Rhea" id="RHEA:28350"/>
            <dbReference type="ChEBI" id="CHEBI:61457"/>
            <dbReference type="ChEBI" id="CHEBI:61463"/>
            <dbReference type="EC" id="5.4.99.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RGP family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.30" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044968150.1">
        <property type="nucleotide sequence ID" value="XM_045112215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XUK9"/>
    <dbReference type="SMR" id="M0XUK9"/>
    <dbReference type="FunCoup" id="M0XUK9">
        <property type="interactions" value="1597"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XUK9"/>
    <dbReference type="PaxDb" id="4513-MLOC_63185.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202110"/>
    </dbReference>
    <dbReference type="GeneID" id="123428052"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0167990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0167990.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0167990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0202110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202110"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRG2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XUK9"/>
    <dbReference type="OMA" id="VYTKSDM"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XUK9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052691">
        <property type="term" value="F:UDP-arabinopyranose mutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071669">
        <property type="term" value="P:plant-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033356">
        <property type="term" value="P:UDP-L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004901">
        <property type="entry name" value="RGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037595">
        <property type="entry name" value="RGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682:SF4">
        <property type="entry name" value="UDP-ARABINOPYRANOSE MUTASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682">
        <property type="entry name" value="UDP-ARABINOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03214">
        <property type="entry name" value="RGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016429">
        <property type="entry name" value="UPTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Required for activity" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Required for activity" evidence="7">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (Glc...) arginine" evidence="8">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016429-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016429-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="348" mass="38874" checksum="F948ECB0D5EDDB9A" modified="2013-04-03" version="1">MSLEVQDSEVDIVIAALQPNLTSFFQAWQPFFSRFDIIVVKDPELAADLQIPSGFNVKVYTKSDIDGLLGATSINFSGHSCRYFGYLVSRKKYVISIDDNCLPAKDPAGMTVDAVTQHMVNLKTPATPFFFNTLYDPYRKGADFVRGYPFSLREGVECMLSCGLWLHNADYDPMTHVVKRNQRNTNYVDAVMTVPLGAMFPVSGINVAFNREVLGPVMFPGLRIRKEGKHRWDTLEDIWNGLCAKVICDSLGYGVKTGLPYVMRSDAEAGKALESLKEWEGVKVMDDVLPFFESLKLSRTAVTVDDCIKELAGIVRQKLAPKNAIFSKAADAMEEWTKLWKSHGAQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0YNL3</accession>
    <name>M0YNL3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123398491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948890.1">
        <property type="nucleotide sequence ID" value="XM_045092955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YNL3"/>
    <dbReference type="SMR" id="M0YNL3"/>
    <dbReference type="FunCoup" id="M0YNL3">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0YNL3"/>
    <dbReference type="PaxDb" id="4513-MLOC_71194.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513320"/>
    </dbReference>
    <dbReference type="GeneID" id="123398491"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0426500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0426500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0426500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0513320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398491"/>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YNL3"/>
    <dbReference type="OMA" id="MASKWFT"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000330">
        <property type="entry name" value="Putative calcium-binding protein CML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="189" mass="20255" checksum="2958C652836F8B31" modified="2013-04-03" version="1">MANVSVSKPAKRLTGKSSFRLGLPLLCGRSDVASPGAAAPRSSSSSSSRRSSGTGSSRKSELRRIFQHFDRDNDGKISGAELSAFFASMGDDLTVPSSSSGEGYLLDFAGFVALMERGEGSQEEDLKRAFEVFNAVDQPAGRITARGLRRVLAQLGDDRSVADCEAMIRAYDVDGDGGLDFHEFQRMMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>M0YRE9</accession>
    <name>M0YRE9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123439309</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971979.1">
        <property type="nucleotide sequence ID" value="XM_045116044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YRE9"/>
    <dbReference type="SMR" id="M0YRE9"/>
    <dbReference type="FunCoup" id="M0YRE9">
        <property type="interactions" value="539"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_72017.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229050"/>
    </dbReference>
    <dbReference type="GeneID" id="123439309"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0189950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0189950.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0189950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439309"/>
    <dbReference type="eggNOG" id="ENOG502R3HK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YRE9"/>
    <dbReference type="OMA" id="YEACIDK"/>
    <dbReference type="OrthoDB" id="773737at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15795">
        <property type="entry name" value="PMEI-Pla_a_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034088">
        <property type="entry name" value="Pla_a_1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357:SF8">
        <property type="entry name" value="OS01G0111000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357">
        <property type="entry name" value="OS02G0537100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="4">
        <location>
            <begin position="37"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="20144" checksum="3C0C415A5CA3F327" modified="2013-04-03" version="1">MAVGTSSSMLGGALLLLLVVVLSSATDFHDGSSSSAAPLSPLDDLCISLGSQYVTPDVCVAALCVDPSCRSTRGLPELAALAIRLTAANATVAKASIESSLAHATDAETKKAMRSCLQLYAGAVEALQWATWSVAAGQYRGAREAVQAAVYVPSGCDGMAQVALPRENDDFFQMAFVGHAVLAWVQHSIDRLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>M0YRY6</accession>
    <name>M0YRY6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nucleotide-binding protein-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123447725</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980301.1">
        <property type="nucleotide sequence ID" value="XM_045124366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YRY6"/>
    <dbReference type="SMR" id="M0YRY6"/>
    <dbReference type="PaxDb" id="4513-MLOC_72191.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0340960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0340960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0340960"/>
    </dbReference>
    <dbReference type="GeneID" id="123447725"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0284600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0284600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0284600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0340960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0340960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0340960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447725"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CNHESHI"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YRY6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000709">
        <property type="entry name" value="Iron-sulfur protein NUBPL isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0340960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="292" mass="31275" checksum="91D48DD7E0812D77" modified="2013-04-03" version="1">MLRIASRSGLLGGRRCYSAAAKGGLSITGVSDIIAVASGKGGVGKSTTAVNIAVALAKEFKLKVGLLDADIYGPSIPTMMHLHEKPEVSEDMKMIPVENHGVRCMSIGFLVDKDAPIVWRGPMVMSALEKMTRGVAWGNLDVLVVDMPPGTGDAQLSMSQRLRLSGALIVSTPQDIALIDARRGANMFRKVQVPILGLVENMSCFKCPKCGEKSYIFGEGGAQRTAEDMDMEFLGEIPLEIDIRTGSDEGNPIVISSPNSASAQAYLRVAEKVTQRLKDLAEERLMAPEISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2003-10-01" modified="2026-01-28" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>Q7XZK3</accession>
    <name>Q7XZK3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123426156</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2003" name="Eur. J. Biochem." volume="270" first="2633" last="2643">
            <title>Identification, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome.</title>
            <authorList>
                <person name="Maeda K."/>
                <person name="Finnie C."/>
                <person name="Ostergaard O."/>
                <person name="Svensson B."/>
            </authorList>
            <dbReference type="PubMed" id="12787030"/>
            <dbReference type="DOI" id="10.1046/j.1432-1033.2003.03637.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2008" name="Protein Sci." volume="17" first="1015" last="1024">
            <title>Crystal structures of barley thioredoxin h isoforms HvTrxh1 and HvTrxh2 reveal features involved in protein recognition and possibly in discriminating the isoform specificity.</title>
            <authorList>
                <person name="Maeda K."/>
                <person name="Hagglund P."/>
                <person name="Finnie C."/>
                <person name="Svensson B."/>
                <person name="Henriksen A."/>
            </authorList>
            <dbReference type="PubMed" id="18424513"/>
            <dbReference type="DOI" id="10.1110/ps.083460308"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="5">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="6">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the thioredoxin family. Plant H-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AY245454">
        <property type="protein sequence ID" value="AAP72290.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK355811">
        <property type="protein sequence ID" value="BAJ87029.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK373063">
        <property type="protein sequence ID" value="BAK04260.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965866.1">
        <property type="nucleotide sequence ID" value="XM_045109931.1"/>
    </dbReference>
    <dbReference type="PDB" id="2VM1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B/C/D=1-118"/>
    </dbReference>
    <dbReference type="PDB" id="2VM2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C/D=1-118"/>
    </dbReference>
    <dbReference type="PDBsum" id="2VM1"/>
    <dbReference type="PDBsum" id="2VM2"/>
    <dbReference type="AlphaFoldDB" id="Q7XZK3"/>
    <dbReference type="SMR" id="Q7XZK3"/>
    <dbReference type="PaxDb" id="4513-MLOC_72465.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0149410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0149410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0149410"/>
    </dbReference>
    <dbReference type="GeneID" id="123426156"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0122770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0122770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0122770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0149410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0149410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0149410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426156"/>
    <dbReference type="eggNOG" id="KOG0907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CYADWCS"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q7XZK3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000104">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050620">
        <property type="entry name" value="Thioredoxin_H-type-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438:SF453">
        <property type="entry name" value="THIOREDOXIN H4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="16 17" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="9">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="9">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="9">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16 17">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAP72290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ87029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0149410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="16">
        <source>
            <dbReference type="PDB" id="2VM1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="17">
        <source>
            <dbReference type="PDB" id="2VM2"/>
        </source>
    </evidence>
    <sequence length="118" mass="12754" checksum="17125D126A45DE54" modified="2003-10-01" version="1">MAAEEGAVIACHTKQEFDTHMANGKDTGKLVIIDFTASWCGPCRVIAPVFAEYAKKFPGAIFLKVDVDELKDVAEAYNVEAMPTFLFIKDGEKVDSVVGGRKDDIHTKIVALMGSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287JWK1</accession>
    <name>A0A287JWK1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123439132</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971816.1">
        <property type="nucleotide sequence ID" value="XM_045115881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287JWK1"/>
    <dbReference type="SMR" id="A0A287JWK1"/>
    <dbReference type="FunCoup" id="A0A287JWK1">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287JWK1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219750"/>
    </dbReference>
    <dbReference type="GeneID" id="123439132"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0183020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0183020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0183020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439132"/>
    <dbReference type="InParanoid" id="A0A287JWK1"/>
    <dbReference type="OMA" id="HIKAWHR"/>
    <dbReference type="OrthoDB" id="598235at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287JWK1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF931">
        <property type="entry name" value="OS01G0547000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="174"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="428"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="545"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="910" mass="102863" checksum="F3E69A807F1B691D" modified="2017-11-22" version="1">MAEGVVALLIGKLGFALAKEAATLGASLLCKEASALNGLFCEIREAKEELESMQAYLQGAERFKDTDETTGIFVNKIRGFAFEIEDVVDEFTYKLEDTHGGFATKMKKRVKHIKAWRRLTLKLQDIKGRLQGADRRKVRYDMKGVDREGLNNVQSRSAGQSLNLAREEDLVGIEKNKGKLMHWLVGDLEEPGCKIATIWGMGGVGKTTLVHHLYKAVKTNFSISAWITVSSSCQVEELLKQIASQLGVAIGAANTNRDLVEVICNCLQGSKYLIVLDDVWNVDLWFKIRNAFPTESTGRFVITTRIQEVALLATKNCTIKLEPLQIWYAWQLFCNEAFWNNVKKTCPDDLKNLAHQFLGKCGGLPIAIACVGRLLSCRHPTYSQWECLYKELELQLSNNVILNVNIVLKVSLEDLPTHLKNCFLHCTIFPEGCQFGRKRLIRHWIAAGFIKEAGSKTLEEVAEGYLNELVNRSLLQVVEQNCCGRVRRCRMHDIIRVLALAKSEEESFCQFYNGSRPFSTENTRRLSIQNTNMEQPTPLLCATSLRSLHVFNSHLRTDSLEAFLKPFNLLSTLDLQGVQIKRLPKTVFNLFNLRFLGLRDTQIEYLPKEIGRLQNLEVLDAYNAMLSVLPVEVATLRKLKYLYVLTIPSGANERVLTFDGIQVPKCIGNLTDLLALQLIEANSEVLCQLGCLTNLRTFAIAKVRSGHCVDLCWAITKMVHLVHITILALDQREVLQLEALCLPPTISKVDISAQLDKTILPQFISSISKLINLTYLTLCWSKLHEDSFACLLGLHGLVTLHLSKAYEGKDLHFHATSLPKLKILGIWDAPNLSRVIIEKGAMPNIVDLFLRDCPELKDLPHGIEHLRTLEYLLLRDISEELSQKLQRNEESKEYSEDWIKISHVRRVDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287L8F9</accession>
    <name>A0A287L8F9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123444014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976543.1">
        <property type="nucleotide sequence ID" value="XM_045120608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287L8F9"/>
    <dbReference type="SMR" id="A0A287L8F9"/>
    <dbReference type="FunCoup" id="A0A287L8F9">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287L8F9"/>
    <dbReference type="PaxDb" id="4513-MLOC_15219.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277100"/>
    </dbReference>
    <dbReference type="GeneID" id="123444014"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0229550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0229550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0229550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444014"/>
    <dbReference type="eggNOG" id="ENOG502QV3A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287L8F9"/>
    <dbReference type="OMA" id="RHCRTEP"/>
    <dbReference type="OrthoDB" id="1909968at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287L8F9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF69">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="305" mass="32300" checksum="C1DDCBF10AE60FEF" modified="2017-11-22" version="1">MEAPDEEAGLGLPEDERLLEVTIISAQGLKPPSGLRRRLQAYAVAWVDTAHRLQTQPDRSGGLDPAWHERFLFRVHEAALAEDSRAAVTVEIYASPNGAWHIGGDSLIGSARFLLGDNCLLSRPVGSPAMFAVGVRRPSGRVHGLLNVAASLVAAPPSPAASHALSLSPAVSLSGLPPAVAISSLSTAPISGRVLRVLNRAHPTPPPSPKVLTPKKLQTSVKPNNKGSDNQQVAVKPNNKREDDASDQEGEDENTYMGGVMFCGPCVLPFPRKIHTSPSDENLQAFAGIFSSGVGIARRSPSPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287SNJ7</accession>
    <name>A0A287SNJ7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287SNJ7"/>
    <dbReference type="SMR" id="A0A287SNJ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0521670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF438">
        <property type="entry name" value="OS08G0128700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="256" mass="28140" checksum="B545C4CFBB002187" modified="2017-11-22" version="1">MEACKTVSACTAGAAEATHVFDILGYSKLRGMGNKPESYIRSGIFAVGGYEWAIRFYPDGNGKESQDYISVYVELLSKSTKVRASCDLKLMDHCTGLSSSMHKTEPRIFNSGDVTAFAPQTSEFQRRSDIEGSSYLKGDHLKIECIVTVFDKPHVTDTRPGLCKIGMPPSDMTEHVGRLLEDKEGFDVSFIVGGETIEAHRLVLAMRSPVLKAELYGPVREARPGQCITIKDMQPGPCSISSIPILCLVARMRIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WEZ9</accession>
    <name>A0A8I6WEZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123421212</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="RefSeq" id="XP_044963458.1">
        <property type="nucleotide sequence ID" value="XM_045107523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WEZ9"/>
    <dbReference type="SMR" id="A0A8I6WEZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017620"/>
    </dbReference>
    <dbReference type="GeneID" id="123421212"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0013640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0013640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0013640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0017620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123421212"/>
    <dbReference type="OMA" id="MKSKCPP"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980320.1">
        <property type="nucleotide sequence ID" value="XM_045124385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WID9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078910"/>
    </dbReference>
    <dbReference type="GeneID" id="123447735"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447735"/>
    <dbReference type="OMA" id="HEPFWSE"/>
    <dbReference type="OrthoDB" id="10267859at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044802">
        <property type="entry name" value="NADKc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010488">
        <property type="entry name" value="Zeta_toxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31153">
        <property type="entry name" value="CALMODULIN CALCIUM-DEPENDENT NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31153:SF21">
        <property type="entry name" value="OS05G0508800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06414">
        <property type="entry name" value="Zeta_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zeta toxin" evidence="3">
        <location>
            <begin position="176"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="496" mass="55073" checksum="38097D02E91368D9" modified="2022-05-25" version="1">MRDDGPAAALVPRLAVSSASRVEELERFSHYVARQIGFDHASECPHLCTLAYDYLRKNKGYEENIFAFFQHTPDPESLVVKFIEELDKCILGYFSFHWKCSTYMITQVLTVEGAPKRKLRNMVLEATRSQRFERVTRNLKVTRLFSTLVEELKAIGLSPPDQAQCSDVMVPVAHCDRSPVLLLMGGGMGAGKSTVLKDILKEPFWSGAAANAVVVEADAFKETDVIYRAISSRGHHNDMLQTAELVHQSSMDAASSLLVTALNEGRDVIMDGTLSWEPFFQQTVAMARAVHRQRYRMGVGYKVTEDGSITEDYWEPVEACSIDEETEMRSRKPYRIELVGVVCDAYLAVVRGIRRAVITGRAVRVKSQLQSHKRFATAFRGYCGVVDNARLYSTNSMGAPKLIGWKDGESNLLVDPKEIGCLERVSGLNDEANCVDELYPEGEPSPSAWQDLVASPSRASVQRELRAAVQTSEARFRAASLADGALRGGDAPVQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIS6</accession>
    <name>A0A8I6WIS6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123449152</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982195.1">
        <property type="nucleotide sequence ID" value="XM_045126260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIS6"/>
    <dbReference type="SMR" id="A0A8I6WIS6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083610"/>
    </dbReference>
    <dbReference type="GeneID" id="123449152"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0068690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0068690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0068690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449152"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000577">
        <property type="entry name" value="Homeobox-leucine zipper protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF21">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HOX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00340">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="133"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="135"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="298" mass="32600" checksum="DF79DDA43B26BCC7" modified="2022-05-25" version="1">MELELSLGDLPAPVKANTMPAPATCQGEDHDDLALGLGVTATERADQDNQRTSMETLEGEADSDEACPKLSVRASPLRQTASANLRGFDVNAAIPVDGRASMARSSSPPSMHMEVPVTQGVDEEASEDEENGGGRVRKKLRLSKEQSAFLEDSFKEHNTLTLEQKSRLANRLSLRPRQVEVWFQNRRARTKLKQTEADCEHLKRHCEALARENRKLQREVAELRAYGRAPYPPYNHHLLSGFSTVLPAACPSCVATTHSTIAAVSSPGPAASTLFPRPRFGAFTAGHPVLRRQPSTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLG1</accession>
    <name>A0A8I6WLG1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLG1"/>
    <dbReference type="SMR" id="A0A8I6WLG1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0074470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0074470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0074470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0074470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0074470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0074470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0074470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="220" mass="24349" checksum="F97B25A542FDBE1D" modified="2022-05-25" version="1">MYDLYAMAHTASYKKVKAFSESDLEHPENFTNISSHDKLVKYRDHGKARKGEDFNPSEGPIDPELVMIACNGRPHGSIAIGDGLIRCPSTLRQIKARQTSSCPEITRRPRPVELAIETAIQKEREAMQAAMAKKDKEIRELEERTTALVEAERARNDASTRAIYELFVSMCEKNGQVPPPMPIINVAGTNISRNASHDPSTVEPSPGQPSPGETSQSHRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQ67</accession>
    <name>A0A8I6WQ67_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ67"/>
    <dbReference type="SMR" id="A0A8I6WQ67"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174190.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174190.1.CDS1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="26829" checksum="9DFBB0122D0DB2E5" modified="2022-05-25" version="1">MATFIPVKLTEFCQPDATMPFGKELVQITKDLRIALPTITGKPTSSYPEDSRYRIEVHIPGRTSQPRTEPVDFKFIAPNWILGRDMAIHCALGRIKEEYKGCPISPDLFTVSRRNKDGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLPLHELELEEAAREAHYEHELEVKDFLERIEKLKTRVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPITMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR70</accession>
    <name>A0A8I6WR70_HORVV</name>
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            <fullName evidence="8">PQ-loop repeat family protein / transmembrane family protein</fullName>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044975787.1">
        <property type="nucleotide sequence ID" value="XM_045119852.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975788.1">
        <property type="nucleotide sequence ID" value="XM_045119853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255700"/>
    </dbReference>
    <dbReference type="GeneID" id="123443476"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0212240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0212240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0212240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0255700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443476"/>
    <dbReference type="OMA" id="NDRLEWI"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="PQ-loop repeat family protein / transmembrane family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF44">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="45316" checksum="BE4880319B7F2EDD" modified="2022-05-25" version="1">MGIFSGTPPPSCPSAPHCAEWAKVYLKYCLCSTKDGVALGLGLASVLSWGIAEVPQIITNYKQKSTEGLSIAFLMTWIVGDLFNLAGCFLEPATLPTQFYMALLYTITTVILTGQTIYYSHIYHLKVKKTGTTAKSQKHQRGDASLREKLLGHRDEAFKNNIQSGPTIPIPSSPILVNTEVFRQRHGSVSPNSDYYYASARSLSRSPVPTGTWLGNNRQTTKFPPQMNDEGESLFGESVPQSAPAAVTKNSLLVVPWISVVLGMCVLHILVGTAQRDASNEIIIPVGRKLLVLADDHAGSSLSHGSGSGIGSFLGWAMAVIYMGGRLPQIFLNMQRGHAEGLSPLMFTFALVGNTTYVGSILVNSLDWARLRPNLPWLVDAGGCVLLDSFIIFQFLYFHYRKQSARDELDNLDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYF9</accession>
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        <recommendedName>
            <fullName evidence="8">Beta-glucosidase</fullName>
        </recommendedName>
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        <name evidence="6" type="primary">LOC123439752</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972370.1">
        <property type="nucleotide sequence ID" value="XM_045116435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYF9"/>
    <dbReference type="SMR" id="A0A8I6WYF9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0252990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0252990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0252990"/>
    </dbReference>
    <dbReference type="GeneID" id="123439752"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0209980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0209980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0209980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0252990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0252990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0252990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439752"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000003">
        <property type="entry name" value="Beta-D-glucan exohydrolase isoenzyme ExoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000002">
        <property type="entry name" value="Glycosyl hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051915">
        <property type="entry name" value="Cellulose_Degrad_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620:SF23">
        <property type="entry name" value="OS02G0131400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620">
        <property type="entry name" value="PERIPLASMIC BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035277857" description="Beta-glucosidase" evidence="3">
        <location>
            <begin position="28"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="4">
        <location>
            <begin position="51"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 3 C-terminal" evidence="5">
        <location>
            <begin position="417"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0252990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="69027" checksum="88F5C3C5CC358730" modified="2022-05-25" version="1">MAFIGSYRMSVLLLLTCTSCILTAAAADYAKYKDPKQPLNRRIDDLLARMTVAEKIGQMSQIERENATADVVQEFFIGSVLSGGGSVPATNATPEAWVRMVNEMQRGALSTRLGIPMLYGIDAVHGHGNVYRATIFPHNVGLGCTRDPELAKKIGAAVALEVRATGIPYVFAPCVAVCRDPRWGRCYESFSEDPKLVQQMASVISGLQGEIPAGGRLGAPYVAAGHRNVAACSKHYVGDGGTAGGANEGDTAATFHDLLSVHMPPYYSAVAQGVSTVMVSFSSWNGTKMHANRFLITDFLKNTLRFRGFVISDWGGIERITTPKGADYMLSVKLAIMAGIDMIMIPYTYTEFIDDLSTLVQNGTIPMSRIDDAVWRILRVKFTMGLFENPYADFSLAGELGKQEHRDLAREAVRKSLVLLKNGKAGEKPLLPLPKNNAGSILVAGSHAHNLGNQCGGWTITWQGIAGNNLTTGKTILDGIKHAVGHGTDVVYSENPDDGFMRQNKARFDYAVIVVGEPPYAESFGDNLNLTVPAPGPSVIRDVCGSVRCAAVLVSGRPLVVEPYMDAIDALVAAWLPGTEGQGVSDVLFGDYGFTGRLARTWFRSVEQLPMNVGDARYDPLFPFGFGLETRAAADIEALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYM2</accession>
    <name>A0A8I6WYM2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123439301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971974.1">
        <property type="nucleotide sequence ID" value="XM_045116039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYM2"/>
    <dbReference type="SMR" id="A0A8I6WYM2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228370"/>
    </dbReference>
    <dbReference type="GeneID" id="123439301"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0228370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0228370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439301"/>
    <dbReference type="OrthoDB" id="597802at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057202">
        <property type="entry name" value="DUF7880"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074:SF136">
        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25306">
        <property type="entry name" value="DUF7880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="220"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7880" evidence="3">
        <location>
            <begin position="439"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="650" mass="73793" checksum="6D4D92FCD9319B27" modified="2022-05-25" version="1">MSPLPPLSFHPSALVGAKAAGAASGFPEWAFLCEEPRFSERRNETTARCRTSEGQAVEVSFWLVDPPGVSYFTFNCPGLDPSVFHEKNPPSLVCAEAAFVLFTLSFGGSIHHFVYTAAPAGKQSLQLLPDLDPDSTLFRRSGFGLLPLADGRQHYAVVYIDRDCFARDFDWWFHAHVYSSETRAWSRHRLSLRHLSQSDKLMCCRHGLSRQVIVAGSLGWVDLMRGILLLCNLLDGNPVIKFIPFPESRVSFLDDDGHPEYAPQYFCNVACYNDVIKFIEVEFDDPAVRTSGQGWRATVWNRKISSDKWEICSRVDVADISVDQSFAAVLPGLWCDETQKLDLKKLILYIPLPSRRDDHLFYMMAKVNVDDDTAWAIAVDMERAAVEAIAPFSLGRYKYHLAMYCACDFPKYLNMTPGADNDNPADKCSKRMTAARLSAKQCVVQVLRTLDWLQELDQCLEIERSTDSTYRLLLQFSPVSSLRSSIRPMVKHASYNGQREAAWKAVDFCLRALGDFDLALHGSPSEPSASVEVMRSKISDVIQALDNIMQILPSTLIRKERMLGDASDQRRSKATSETCEKPIETKNTFRGWLQVIFKPIHQERYQHRRRWRKASREQRAVVDTNDTHDKWQQGKFKHGNSRREKAIGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X242</accession>
    <name>A0A8I6X242_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X242"/>
    <dbReference type="SMR" id="A0A8I6X242"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0113640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0113640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0113640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0093380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0093380.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0093380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0113640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0113640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0113640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF13">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0113640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="129" mass="15540" checksum="C39D940EF9E54782" modified="2022-05-25" version="1">MHREQNLGRVKDKQAHEKEVGKLKKKIDFLSNSYSQLVEDVSKFFDYQDSKMFHDMEYTSEAIIDLNKKKKELEDQAKIEISMEKLKLAKQQRCILQSQEDIIQNTRKAMKEVQGTWTYLRKRIRRWSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4H3</accession>
    <name>A0A8I6X4H3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4H3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0065810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0065810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0065810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0065810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0065810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0065810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0065810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="211" mass="24440" checksum="633C1037F8B2ECC8" modified="2022-05-25" version="1">MDQRYVDVTKSRNKHDNTTVIHHYKVDVFNVAIDQQVIELNDRFSSQVTELLDLCSSLDPRHDAFDKSKICTLVEKIYHAYFSSQERDRLECELPHFQLDTFNHREIKNCKSLADMTKGIIKTGKSSDYPMVERLLRLVIALPVSTATGERAFSAMKLVKTRLQSKLGDDFLRHCTIVYIEKEIAAKFSSDDIIEIFDTRARKSSFKLIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAW1</accession>
    <name>A0A8I6XAW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAW1"/>
    <dbReference type="SMR" id="A0A8I6XAW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0155690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0155690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0155690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0188010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188010"/>
    </dbReference>
    <dbReference type="OMA" id="RCEAGIC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035204309" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="42931" checksum="2A077BA10967773E" modified="2022-05-25" version="1">MGIIGILRPAILLLAGLAWCSELVAAAGRVDGVFNVKEYGARGDGTTDDTKAFVDAWTAACRARGSPATLLVPAANSFIVGPTRFSGPCASTRITVQVMGTITAPPAGAWRELEYWLMFYQVHGLTVTGGSTGLLDGRGGTWWRKKCKDHADCISRAPTGLVVMNCIDVELSQFHSKDSPQMHIAVSMSSNVNVAQLTITAPWDSPNTDGVHIDRSDHVRVTGSTIGTGDDCVSIGSGTRFVTVDGIVCGPGHGVSVGSLGKKGAAVSVEYIDVRNVQFINTMNGARIKTWQGGKGYAKSISFTDINFTNVEHPVVINQFYVDRHHVPNNMGAVALSNITYTNLSGTSRRKTAVEFDCSESGSCTNIHVNSVAITAADGGGTVARCRNVQGDTSGYVYPKIPCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAW5</accession>
    <name>A0A8I6XAW5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123429002</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969009.1">
        <property type="nucleotide sequence ID" value="XM_045113074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAW5"/>
    <dbReference type="SMR" id="A0A8I6XAW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0095910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0095910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0095910"/>
    </dbReference>
    <dbReference type="GeneID" id="123429002"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0079140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0079140.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0079140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0095910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0095910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0095910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429002"/>
    <dbReference type="OrthoDB" id="407275at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="75"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="516"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0095910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="539" mass="59673" checksum="E5FCF940CF6153CA" modified="2022-05-25" version="1">MARSFNSLAPVLITFSCFLCLLGCYYVISSSSQASSDGFLGCLTDNNVPVFTQSSHFFMPLLNASIRNPKFFVNTTVRPLYIVMPTTTPHVQAAVRCGSGNNMSIRVRSGGHDYEGLSYRSVDAGGFAMLDMSELRTIVVDNQTSTAWVESGATLGELYYKIGNDSNMLGFPAGVCPTVGIGGHFSGGGFGMMMRKYGLSIDNIINAELVDAKGRLLNKTTMGEDVFWAIRGGGGGSFGVVVKWQVTLIPVPPSVTVFNVSVSLGQGAVDVVTKWQQVAPTMPRDLFIRVLIKNKRADFQALFLGTCEALLPVMNKNFPELRFNRSDCREMTWLQSAPYIYLGKDSSVEDLLKRNTSTFFSTYGFKATTDYVREEKPIPRDVWAGIFHKLAQPEARDARVILDPYGGFMSDVPDSATPFPHRAGVLYSIQFYNYWPVYRESGEMQINWIKDVYAFMAPYVSSNPREAYFNCRDLDLGTNWGAKYFMGNYQKLTKAKGEIDPLEYFRNEQSIPLQGNITKMRASPDDDAEAGDGGSASSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFN6</accession>
    <name>A0A8I6XFN6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123428434</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044968581.1">
        <property type="nucleotide sequence ID" value="XM_045112646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFN6"/>
    <dbReference type="SMR" id="A0A8I6XFN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0212850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0212850"/>
    </dbReference>
    <dbReference type="GeneID" id="123428434"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0177000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0177000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0177000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0212850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0212850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0212850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428434"/>
    <dbReference type="OrthoDB" id="599913at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase U9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF543">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="93"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="347" mass="38044" checksum="AFF2BF47B8E88DB8" modified="2022-05-25" version="1">MAGGDNLKLVGMWASPHVLRVGLALRLKGLSYKFVEMEGEEGFKNNQGEQLLLNSKLQVLIHGGKSIGGTSLSMIQYIDEAFPGAGPSLLPDDPSERAMARYWADFIDDTLVRAMHKAAWGESDREKAEGKNHVVSAVETLEGALRDCSKPFFGGNSVGYVDVVLGGLLGWVRATDTVQGVKTFDDPAITPLLAMWAYRFDALKTVQVVMPDVGALVDFAMTMPSHSSGRLGIVVSIYLIAVLLQAVASYLTWLGRAESKGEPQDGNPCSRLQWLVPRALPYWSCYVLEGLDFVTRSMPTLFLLYVFVKGFKSSIVRVLVMGVPVVVITWYFLTFYKPCGHGTLNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIF0</accession>
    <name>A0A8I6XIF0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIF0"/>
    <dbReference type="SMR" id="A0A8I6XIF0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0410360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0410360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0410360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0410360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0410360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0410360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0410360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="15059" checksum="7A860A0CB8AC5854" modified="2022-05-25" version="1">MEFFTIILEKSSSRQVLPDNFVKMLDNHRPRNMKLRQADNGLRKLWDVELVFDTDGSMYLDRGWKQFVRAYDLRHGYFLVFRYDGNATFAVKVFDTTMCWRHYQDDNDASVLCLFFLSIRLCLTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIN3</accession>
    <name>A0A8I6XIN3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Succinate dehydrogenase subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of complex II composed of eight subunits in plants: four classical SDH subunits SDH1, SDH2, SDH3 and SDH4 (a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.), as well as four subunits unknown in mitochondria from bacteria and heterotrophic eukaryotes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIN3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0215330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000010">
        <property type="entry name" value="Succinate dehydrogenase subunit 3-1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF18">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 3-1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215330.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10032" checksum="92B8B6A15C399DC6" modified="2022-05-25" version="1">MDGLTLSSSIGQAKSYASTPFGAVRQKMPPSGSRSLHTSRPLSAAVANRPLSPHLPLKKPQLSATFSISHRIFGVALGAAIISIPLATKFSLMFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLN1</accession>
    <name>A0A8I6XLN1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123398763</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044949153.1">
        <property type="nucleotide sequence ID" value="XM_045093218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLN1"/>
    <dbReference type="SMR" id="A0A8I6XLN1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517730"/>
    </dbReference>
    <dbReference type="GeneID" id="123398763"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0430260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0430260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0430260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398763"/>
    <dbReference type="OrthoDB" id="657271at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009610">
        <property type="term" value="P:response to symbiotic fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000067">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF408">
        <property type="entry name" value="PHYTOCYANIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188003" description="Phytocyanin domain-containing protein" evidence="13">
        <location>
            <begin position="23"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="14">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="160" mass="17065" checksum="9FD1C08C057D982A" modified="2022-05-25" version="1">MASKQMLAVAVAIAMVFLPLLAASATVHPVGDGSGWTLGFHYTTWSESKQFTVGDALVFKYNKALHNVVEVSGPDFKACKDTKGAAAWRSGADQVHLGDAGRRWFICTVRNHCQMGMKLNVTILAADALSPAPAPAPAPSSTVPSSSTPHKSRRPFVSKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM25</accession>
    <name>A0A8I6XM25_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">Acidic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123451094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984040.1">
        <property type="nucleotide sequence ID" value="XM_045128105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XM25"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339960"/>
    </dbReference>
    <dbReference type="GeneID" id="123451094"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0283680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0283680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0283680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0339960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451094"/>
    <dbReference type="OrthoDB" id="672603at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051391">
        <property type="entry name" value="Protease_inhibitor_I20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33832:SF27">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33832">
        <property type="entry name" value="SERINE-TYPE ENDOPEPTIDASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035265253" description="Acidic protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="8933" checksum="0AECCC19546BF110" modified="2022-05-25" version="1">MVSAMKPSVTFVLLLSGLVVFGVIADAKAPCAVVCFQGGHITCDNYPGQELDGCDCECAPADGKGCVLHLDDGVTKTNCRRPKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMM8</accession>
    <name>A0A8I6XMM8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMM8"/>
    <dbReference type="SMR" id="A0A8I6XMM8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421720"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF92">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421720.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24994" checksum="CDB9A5096BC94EC4" modified="2022-05-25" version="1">MVLNCELDNRWVVPYNPYLLRMLNCHINVEICSSIKAVKYLFKYIYKGHDRASVTVTDKADEVEIDEIKQYRDARWVTPPETLWRIYGFELSKIHPPVLQLQLHLPNMHMVSYHNMKKIKNVIDREGTERSMLTAYFEANSLYENARGILYREFPKHYNWQSREKLWKQRKRAAVFQVGRIVSAHPAEGERYYLRVLLNHVTGATSYEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNF9</accession>
    <name>A0A8I6XNF9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNF9"/>
    <dbReference type="SMR" id="A0A8I6XNF9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480810"/>
    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="228" mass="24697" checksum="F42428BF92056B12" modified="2022-05-25" version="1">MPHLYDLYGMAHTASYKKAKAFSESGLDDPNNFTNISSHHKLVACRDAGKPMKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKARQSSGCPEITRRPWPVDLAIEAALQKERLANQAALEKERLANQAALGKERLASQAALQAALDERDQTMARLIEEERSRNEAGQRALYEIFVGLCEKSGQVPPPMPVFSLIGINNSRAASHNPSPGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTG3</accession>
    <name>A0A8I6XTG3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTG3"/>
    <dbReference type="SMR" id="A0A8I6XTG3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0315750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0315750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0315750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0315750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0315750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0315750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="1"/>
            <end position="98"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="18"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0315750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="26810" checksum="B63A93088CBD7881" modified="2022-05-25" version="1">MNKKGKIKKGRPFTLPRWYEELKDDEKWKKHDDIDDVDESNKRNRTIELDHDEEEESSDVGKRSPTPNSVSYSKPKRPDGCKNDAKEKKKRKGDDELKNAMEAIVKARKEANEVRKMARTQDAAAGERRLAAEERRVAAEDRKVTLDDRKVGMEERSRLLEWEKHLFFLDTSTLNEVQKEYVNLTREEVLIQKRAMIRGMGGPGIGGMSGFGVTMGGMGAMGGFGATMGGFGAIMRS</sequence>
</entry>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </feature>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="10" key="1">
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        </source>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
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        <property type="nucleotide sequence ID" value="XM_045125212.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333110.1"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670">
        <property type="entry name" value="HEAT SHOCK PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670:SF89">
        <property type="entry name" value="OS03G0656000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="8">
        <location>
            <begin position="14"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="258" mass="27454" checksum="7C30559381ACBC55" modified="2022-05-25" version="1">MQAAAATAPAPRHRVYTAVEPRCEWARAADADTLVVDVSGFVKEELRVLYNTSRKLKVAGERPVGDGGNGGARWARFLKSFPVPKSVRTGGIRAVMDKDQAVLYVILPKGSPPTTTGPPPPPPPPPLSSAMATEEQQPGSAMPPPHGERKGDGSSGGSSSSSNGSFWSAQEDAGKNRAEEKNMETKIQIQEEEIATADTPRTDGGSDANTVEKDGGDDEGDDKRWWEKMKLRPLHVVGFVVLVLAVVGIGALYAMLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XX58</accession>
    <name>A0A8I6XX58_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123447761</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980349.1">
        <property type="nucleotide sequence ID" value="XM_045124414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XX58"/>
    <dbReference type="SMR" id="A0A8I6XX58"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341950"/>
    </dbReference>
    <dbReference type="GeneID" id="123447761"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0285380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0285380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0285380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0341950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447761"/>
    <dbReference type="OrthoDB" id="45882at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11369">
        <property type="entry name" value="RNase_PH_RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000018">
        <property type="entry name" value="Exosome complex exonuclease RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033196">
        <property type="entry name" value="Rrp43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF9">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="48"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="219"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="306" mass="32406" checksum="B7DC6384EF7BDFE5" modified="2022-05-25" version="1">MAAETDATAPGGLAGEMEVEAYRRLFPLAFLERHLRESVRPDARRLAEARPTTVALGAVSSAHGSALVRLGDTAMLASIKLEVMSPPAETPDQGSVAVEFHMPPICSPLVRPGRPAEAAPVISKALEDVLMSSGMLNLKELCLISGKASWVAYLDVYCLNADGSLFDAALISVVAAFTHLEIPLVSVGDDGRVFTVGGNEGKTKYELVNREKRKLTLGDIPFPLTCALHKDSILADPTAEEESIIETSVTVVLDSSGRLASIQKPGGGVTSMAAIKACIGLAKERGQKLKEILTDSIEAMEVDQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXM4</accession>
    <name>A0A8I6XXM4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXM4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24826" checksum="2AEB4C35D06619E1" modified="2022-05-25" version="1">MPHLYDVYGMAHTASYKKAKAFSESDLDDPSNFTNISSHQKLVAYRDAGKATKGDDFNPSEEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKARQSSNCPEITRRPRPVELAIEAALQKERAANQAALEKERLASQAALQVALDERDQSTARLLEEERARNEAGQRALYELFVGLCEKSEQVPPPMPVFSSIGTNNSRAASHDPSPGQPSPNEAGASNRGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXS0</accession>
    <name>A0A8I6XXS0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123403784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">subsp. vulgare</strain>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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        <location>
            <position position="94"/>
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        <ligand>
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        <ligand>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y198</accession>
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        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Triticodae</taxon>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045099030.1"/>
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    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0578740.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="14070" checksum="80FF94DA7D7FC43B" modified="2022-05-25" version="1">MPRHLGLHGAIAAGASRHLALHHGGVIAAAAPAAAAFLAVCALALALCASHSGAGAAATERLRRALASVSRRRTDPVISIHQVQPGGVGADASPPPCVWQKGILMGGKCQLPDFSGVINYDPAGNMVAPGRPRAVPALGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1Q8</accession>
    <name>A0A8I6Y1Q8_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="6" type="primary">LOC123446986</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045123536.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6Y1Q8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0401210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0401210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0401210"/>
    </dbReference>
    <dbReference type="GeneID" id="123446986"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0333890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0333890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0333890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0401210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0401210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0401210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446986"/>
    <dbReference type="OrthoDB" id="687020at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000003">
        <property type="entry name" value="Blue copper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF245">
        <property type="entry name" value="PHYTOCYANIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035219791" description="Phytocyanin domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="5">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0401210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="176" mass="17652" checksum="8F6BDDA188AF89E4" modified="2022-05-25" version="1">MAAMKIALLAVAAISALLLGTASAATYGVGEPGSSWALGTDYSKWVSNKKFHPNDEIVFKYSTPTHDVVEVSKAGYDSCSAANAINTLTSGNDVITLNTTGTRYFICGVPNHCSPTSAASMKVVIEVVPASSSPSSPMPAAGPDASNPPPPSSTASSVGATAGFGLVALLSAGLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y249</accession>
    <name>A0A8I6Y249_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y249"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0405120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF8">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="1">
        <location>
            <begin position="12"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="197" mass="22896" checksum="9CB05AF46F590655" modified="2022-05-25" version="1">MQFLSEEEAYLFYNKYAYSLGFSIRRGNQHKVKNSSTIQQRTFTCSRQGVRAEDKREEKFNYSRPETRCGCDARMKISLTNGIYYVYEFKEAHNHSLATGNMSQYLRSHRKVTEAQLANAEVAKSVGISNKATIDLMTKEACGPENLGFISTDMKNRLYSKRTLKAKVGDTGGVLEWRRRFQKMSIFFIQFKLMRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y495</accession>
    <name>A0A8I6Y495_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal ATPase PEX1 N-terminal C-lobe domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y495"/>
    <dbReference type="SMR" id="A0A8I6Y495"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0411300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0411300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0411300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0495930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000006">
        <property type="entry name" value="Peroxisome biogenesis factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxisomal ATPase PEX1 N-terminal C-lobe" evidence="4">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="592" mass="65466" checksum="1120312813E1E4C8" modified="2022-05-25" version="1">MEVVVRTVPGVRSCFVALPLPAIQALERTTAAGSLPAVLALELRAPDGARWRLAWAGAVSASPDAVEVSQQFAACISLPDNTKASLSAVSVLPKAKFVSVEPISEEDWEVLELNSELAEEAILKQVGIVYDGMKFPLWLHGDNVVEFLVISASPSNSIVQLVPGTEVAVAPKNRKGDANSNQYAPKQDLLKGQPHKKALLRVQEAGGKYVDRFEYNGIQLGVFVTYAVQIHPDTAVKLSLSNLQLVSITPKFSPKESTENGKGSDQQIKGSVPGMKRSRHIVVHIMLSDSVAKEHIMLPQSIRCYIGTCVHSWVYVQRFSPALNKNTPSITIRPLHFKMPERNSEDNSALDCQEQDICQTLGEISLAKDPPTDDNVSMSHGFNDITAAANWEESAKLKQERSFIRSWLIGQLKGISSQIAQPELNTVLLPSETLLHFQVIDQTRGSPVDLIYLLTTTSDFGVNHKKIELVMATEYGIDNFEQALQKLELNKAISLDSTTEREKIFFYRGLSSLSWLENEFSDIEKRLNVLLSSASLKLLSRIKCPYPGNVLVYGPPVSTPSYLLRLIELLYESGTKYLFCTRDLCRLLKASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y516</accession>
    <name>A0A8I6Y516_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y516"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0593880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0593880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0593880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0593880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0593880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0593880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47127">
        <property type="entry name" value="10A19I.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="2">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0593880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="297" mass="34332" checksum="A42A3014CF999FEA" modified="2022-05-25" version="1">MRREYFDNKIWKNDTTSVNMLRLRREPFFRFCQLFRDRKLLKDTIHLSIEQQVAMFLHTVGHNIRNRVICANFGRSGEVVSRYFQRVLHAIGELRDDLIRNPSLETQTKIEGNYRWDPYFKAHPEDEQYLKKPIIHYKEMMNIAGGSMVTGQYAKGSSDALATEVVDIEEETSKTHTAPHEAPHEEVEQSTNAGSYGPKPKKAKPNPCAEDRLHATILASSERIAITIEKSTNTEKNAIDGLWESMKVLPGFSLDYLAHYHAYLVDNPRVAMAFKVLEEDQKKVWVSRYVKNTFPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y597</accession>
    <name>A0A8I6Y597_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    </reference>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9N7"/>
    <dbReference type="SMR" id="A0A8I6Y9N7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0634670.1">
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20898" checksum="6799DD6CE64D1648" modified="2022-05-25" version="1">MPTRFGEVLAHGKTKIDVVYTNESREMPYFLGQLKERWLDAAVDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRNGITFATVDITNDKLKMRTLMAHMAVALIDEEYGNMKTNFPKSQHKLWEKAPLDRINIEYAAKDAYVSYELYRKIRVVNYGQRHLE</sequence>
</entry>
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        <submittedName>
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        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        </source>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0577780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="105" mass="11897" checksum="20607546216EB22F" modified="2022-05-25" version="1">MCFETSVEHFHLSINIFCFSLSVVCHVVLKSHITPLLAFASGCIKVENIIISMQQKPITVHVKFPKHKNARKCTSLHPMSQKMLPRQAQMQVKGSIQTPYNSFSL</sequence>
</entry>
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    <accession>A0A8I6YC23</accession>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="OS01G0158850 PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
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    </dbReference>
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            <end position="526"/>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="597" mass="66941" checksum="2CA1787550AF294E" modified="2022-05-25" version="1">MKLLCWNIRGLGLNGRRRRLIEYLRQEEIDIVGLEETIRQDFSMHELHGLSYQQFASQWLPATGQFGGILLGVREDAFSVEYMDRGEFFVSMTVTDRQVHLSWEVIIVYGLVDHGHSADLLAELKNKVEGCTTPVVVAGDFNLIRWDSNKSSPNVDRVRMRLFNDCIADLALREIARVGARFTWSNKQADPIRSVLDRVIVSAQWEVMFPLFSLKVVTRIGSDHTPLLFSSGEEPPLGPVTSSLRCSGWSNRVSLSWINFGVIALIPKVVGASDIRQFRRITVINVVERIFVKVCATRLSPVAERIAHPLQSAFLKGRWIHDGILTLHEIVHEVASKGLKGVFLKLEFQKLYDRPGWSFLRLVMQRRGFDERWCSWIMQLVRSGNTTININGKVGPFFQASRGVKQGDPVSPLLFNLAVDALAGILDKGKLAGHLQGVVGHLIPDGGVTHLQYAHDTMIMVEGSDSDIVHLKFLLLCFEEMSALKINFDKSEVVVLGYSEAEQQKIADNLNCRLASFPISYLGMPLADSMILMSGFDPLVGRVASRAEPWCGRFTSKGSKTILSSSNLASLPMYMMGMYILPKGVHSAFDKELARFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YC72</accession>
    <name>A0A8I6YC72_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BPM/SPOP BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
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    <reference evidence="4" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YC72"/>
    <dbReference type="SMR" id="A0A8I6YC72"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423340.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509700"/>
    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF187">
        <property type="entry name" value="OS07G0655300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPM/SPOP BACK" evidence="3">
        <location>
            <begin position="41"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="111" mass="12372" checksum="DCC70514E16308F8" modified="2022-05-25" version="1">MDQREESAMVQHLLVAADRYALERLKLICEDKLCNRIDTNSVATILALAEQHHCHELKAACLVFLSSTTNLEAAMESEGFEYLTKTCPGVIKDFLISHVVPSLLGKRKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCU6</accession>
    <name>A0A8I6YCU6_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123410139</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958961.1">
        <property type="nucleotide sequence ID" value="XM_045103026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCU6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665520"/>
    </dbReference>
    <dbReference type="GeneID" id="123410139"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0552150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0552150.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0552150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0665520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410139"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017088">
        <property type="entry name" value="Wax_synthase_Magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF73">
        <property type="entry name" value="OS02G0454500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037006">
        <property type="entry name" value="Wax_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="194"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665520.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="358" mass="38669" checksum="7F4BC896A5B4D8E0" modified="2022-05-25" version="1">MAAALMDGELGSLLKVTAAVWAAMYYARMAAALIRPGAARLAALLPAVALFCAIPLAFSTTTFRGTSAFFLCWLGTFKLLLLAAGRGPLDPSLSLPHFVFSASLPVKFRQPASTSAKAKDHQDPASASARGDGPAKILVSGAVIPFIIYAYQFKDAMSWWQLVLMYTAHIYFSLELLLGSVHALVHGVLGMEMEPQVDRPYLASSLRDFWGRRWNLMVPAILRPSVHGPVRARFGDAAGVMASFLVSGIMHEVMFYYIMWQPPSGDVTAFFVLHGACTAAEGWWARHAGWWRPPRAVAVPITLAFVGGTGIWIFFPAMVRGGLDELVLQECQGLVVAMEQAGRWLAGGGAAGPILSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDS4</accession>
    <name>A0A8I6YDS4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123411347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960212.1">
        <property type="nucleotide sequence ID" value="XM_045104277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDS4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0698480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0698480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0698480"/>
    </dbReference>
    <dbReference type="GeneID" id="123411347"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0579540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0579540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0579540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0698480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0698480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0698480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411347"/>
    <dbReference type="OrthoDB" id="2020529at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34572">
        <property type="entry name" value="GOLGIN FAMILY A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34572:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0698480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="201" mass="22111" checksum="41A9BF2F1E56F631" modified="2022-05-25" version="1">MEGVGARLGRSSARYGPAMTFTGPVRKWRKEWVPISAATATASSAGTGSRGNNLILFKWTPFNGSNEGDEEQTAPAKTATRRRRYVPVSVVQDQRQESAKSDDENKANDGEPSSTETEQSIGKTNIDDILMDESQASDEVRDSGNFVGGTDLNLNLGLKDPDVEDEGDTVEDEGDTVEHHRVRTEHRLKRKSVTPDLEMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDT7</accession>
    <name>A0A8I6YDT7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123404373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="SMR" id="A0A8I6YDT7"/>
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    </dbReference>
    <dbReference type="GeneID" id="123404373"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0495420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0495420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0495420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0597680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0597680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0597680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404373"/>
    <dbReference type="OrthoDB" id="615522at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF50">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0597680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="44297" checksum="FEFF0F98A99B2CC5" modified="2022-05-25" version="1">MAPDRLSELPDCLLQGILSPLGARQVLRTAALSRRWRHVWRGVLCLSLDIDQREFQAADAEAATTDGNRRRNKSVVLEEWARFDDFADGILRLDLDRRDGRGGLPLDLDSLRLHVVDDQQASRRRRPRYITWLAWVHGCLDRYRPAALEIVSDFDITVDLRLMGLRSDLSRLKTVRLTGIIHSGGLGDSVCPQLEHLEFTGCAIGFQEAVTSPTLKRLVMDRTWRRGDMKQDSPVLIDAPALTSLRLLLHFNAIWAFQTPSLVEASVRSTRGAWVSRADEYQLLCSLYNVTRLELSGFLALEKIRTSPGHQDLPTFDNLTTLLLDECDLTGEVYNMLELFLNNAPNLEELTLQNCKLPQDWTESKKMCLNLKFREINCHKEGDLPQVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF24</accession>
    <name>A0A8I6YF24_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF24"/>
    <dbReference type="SMR" id="A0A8I6YF24"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0536540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0536540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0536540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0446480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0446480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0446480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0536540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0536540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0536540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF54">
        <property type="entry name" value="OS12G0464400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="337"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0536540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="349" mass="38496" checksum="56905C69D1F4DE2B" modified="2022-05-25" version="1">MDVVNGVLNWAAAPAMLAALLLFYPPYYLFKTCYSFLSYLFPDNLKRKVVLITGASSGIGEQLAYQYAAKGACLALVARREWSLRQVADRAFDLGAPDVIILPGDVADPDDCNRFIQATIDHYGRLDHLVCNAGIASVGAFQEIPDVTNYSSQLDVNFWGAVQSTFAALPHLKRTRGRIVVTASATGWNPVPRMIFYNAANAALINFFETLRAELGSEVGITIVTPGWIESEMSKGKFLKEHGGVEVDQEFRDAQVGLFPVEYAKNCARAMVQAARQGDRYLTVPSWFGTMYLWRVFAPEVIEFCYRLLYMHGHGGGDQTDAPSKTMAQGGGKQMLYPSSLQSSDVKNE</sequence>
</entry>
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    <accession>A0A8I6YIQ5</accession>
    <name>A0A8I6YIQ5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIQ5"/>
    <dbReference type="SMR" id="A0A8I6YIQ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0648850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0648850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0648850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0648850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15892" checksum="2A5F528FF3F88111" modified="2022-05-25" version="1">MVLVSALVGPHRVYESDVEVCESEIPTHLEMLLEKLTLVEKENKYLKEKLKIIEDKNMEMELHVAEVIDDRKMKMDAMRLKMDAMRLKMDEMRLNIRNIRKYAIDKEAWYHYVVGSIVTLLAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJK7</accession>
    <name>A0A8I6YJK7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="4">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="22 23">cv. Morex</strain>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="22">subsp. vulgare</strain>
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        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
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        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
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    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020547">
        <property type="entry name" value="ATP_synth_F1_esu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00401">
        <property type="entry name" value="ATP-synt_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="21">
        <location>
            <begin position="147"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0798500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="539" mass="58324" checksum="EB8330C3B7C0F71E" modified="2022-05-25" version="1">MRTNPTTSRPGVSTSEEKSTGRIDQIIGPVLDVTFPPGKLPYIYNALVVQSRDTADKQINVTCEVQQLLGNNRVRAVAMSATDGLMRGMEVIDTGAPLSVPVGGATLGRIFNVLGEPVDNLGPVDSSATFPIHRSAPAFIELDTKLSIFETGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVINEKNIEESKVALVYGQMNEPPGARMRVGLTALTMAEYFRDVNKQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLSTEMGSLQERIASTKKGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLASKGIYPAVDPLDSTSTMLQPRIVGNEHYETAQRVKETLQRYKELQDIIAILGLDELSEEDRLTVARARKIERFLSQPFFVAEVFTGSPGNGQIGVLPNHAPINTAVDMGPLRIRLLNDQWLTAVLWSGFARIVNNEIIILGNDAELGSDIDPEEAQKALEIAEANLSKAEGTKDLVEAKLALRRARIRIEAVNWIPPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YK78</accession>
    <name>A0A8I6YK78_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044952353.1">
        <property type="nucleotide sequence ID" value="XM_045096418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YK78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0597570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0597570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0597570"/>
    </dbReference>
    <dbReference type="GeneID" id="123402495"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0495310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0495310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0495310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0597570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0597570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0597570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402495"/>
    <dbReference type="OrthoDB" id="613859at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="2">
        <location>
            <begin position="180"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0597570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="54707" checksum="290359F5DB19DB36" modified="2022-05-25" version="1">MKFNRGRRHRPNLKTEVGSGDMPSILPGNIQACNGDRLSELPVDVLLNILERVGTLDAVRTCILSKQMQMLPNMLSQIVIDLSPRDLFQKSHVVADVTNKILSRRCPHITIRMLKLKFVLSPSCCLSIGKSVGLAMTTQKFDAAEFQIMTPKDFHVSTDAHRVLFAKQFNTFVRDCPDAFAGLTRLHVQNMNFGESDIPNILSYCKRLESLSFFMCGAGSSSVLYLEHAQLVELVISYCRFKTVEVKSLPKLQRMTFGDWTCDENPLVLNFVPQLSKLSLANPNFSGKTLNLSKLLANAPTVNDLFLEFRSEKIWVQPECPKVLAPLLAKLRFVNLDHLREECDISWTMFLLEAAPSVEDLCITVWDHKCRRKSHESYSKKTDVKWKPSGPDFKHKNLARLTIYGFQSDDNFTGYVRRVIQAAVNIRKVSLHDRKVCELCSEKFPHAEARPSGYPRTSEEMDLSRKKMAAASATTCPDIHFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YM15</accession>
    <name>A0A8I6YM15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123397591</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YM15"/>
    <dbReference type="SMR" id="A0A8I6YM15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0419550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0419550.1"/>
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</entry>
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            <fullName evidence="5">Bowman-Birk serine protease inhibitors family domain-containing protein</fullName>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">subsp. vulgare</strain>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="105" mass="11542" checksum="F500CA610CEFDE33" modified="2022-05-25" version="1">MKGSSLVFMVTLLSISYLAASGPRDLGDERSRDDGRASASANAPVLHSSLDESKISMRFCVVRDCKTKAEGWTELCYCCLPLPDFPCWHKLHECQANCPACKPNC</sequence>
</entry>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF283">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0528360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="579" mass="64346" checksum="AE3973CFDD525471" modified="2022-05-25" version="1">MAAPPPPPALPPALPDDVVEEILLRLPLDDPGSLLRASLVCKAWRSVVTHPHFRRRFIGLHPHRAPPVLGFLHDWENKRIPDFVPTTPSPFSLAAPDRRFWRPLDCRHGRALFLSDDRQETQEILLWEPITGAHQRIPLPAAFRCGWPTAAVFCTADGCDHRDCAGGPFGVVFVFAVDGSEDEEDWHVITSACLYSSETGTWGKPNSRQDEFTMEFEYHSSVLVGRSLLYFLSDGGVVLEYNLDSPELSVFNTPPDDYGSEKERFNLVLVDDGGLGVAEAIDMELILWKWEASDGTNARWVLSRIVDLFDVLPIGVDSECLSPVLGFAEGANAIFVKTDSSLFMIKLQSEQGEKVCDDHGFCNLIPVVSFYTPHSRLQVPGGEHHGPAAPPLNLLRRGGQQVAWEEKSLEWAQVLFDKGCKAINEKDFANAANCFRDALKIRVQHYGDLAPQCASTFYCYGCALLAKARKATNPSGANLHGKGQKDGNMTGDRDDSDLDLAWKMLNTAKVIVAKSPDKTMEKVNILNSLAQISMRREDTDSAIGYYFEALAILEHLVRPDNFRIFEQYPPCGDNIVNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPR4</accession>
    <name>A0A8I6YPR4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123409835</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958641.1">
        <property type="nucleotide sequence ID" value="XM_045102706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPR4"/>
    <dbReference type="SMR" id="A0A8I6YPR4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635150"/>
    </dbReference>
    <dbReference type="GeneID" id="123409835"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0527690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0527690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0527690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0635150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409835"/>
    <dbReference type="OrthoDB" id="1919458at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000003">
        <property type="entry name" value="NAC domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF230">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="9"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="336" mass="39043" checksum="6B8C5EA3FE26279B" modified="2022-05-25" version="1">MSGSTQPQVPPGFRFHPTDEELVDYYLRKKVASRRIDLNVIKDVDLYKIEPWDLQEKCRIGPEEEQSDWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKPIYAKHCLVGMRKTLVYYKGRAPNGQKSDWIMHEYRLETNENGPPQEEGWVVCRVFKKRLPTTRRDLDHDAPCWYVDDDGPFMHDLNSPMRGMPPHHSMALQEQHLQMLNNTYKRELKLQYQMPNHHHVLNTIPHELESPSSFHSLLVSQDDHQINVHHAQHHHVQLIDHAVDDQAATDWRVLDKFVASQLSNDAAKGVDYAHEGDILQLNEKVQEVATDYASTSTSSSQVDPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQS1</accession>
    <name>A0A8I6YQS1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQS1"/>
    <dbReference type="SMR" id="A0A8I6YQS1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0464960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0464960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0464960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0464960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0464960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0464960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF11">
        <property type="entry name" value="DUF4201 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0464960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="207" mass="23972" checksum="8A4155910CA8EF84" modified="2022-05-25" version="1">MQNALLKLWQMLEDSKSARRNDNLESSLKNHHLTEEKRNLDANYDKLVEDVNQLLNAQVERVMDFSYLQDKVNDAESTSAAVAIMKTEIEKKEAEKMELRGKYSVLMNLVEAQGRLIRNQKANHLKEKEKLSEENYNLKVQVDKLNNSEEKLKDDIGVLNLHIGGLKKGMDNLNKRRDELKLEIADQLKGVEKNQEKLKMIRDILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YS98</accession>
    <name>A0A8I6YS98_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YS98"/>
    <dbReference type="SMR" id="A0A8I6YS98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0561700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0561700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0561700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0561700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20902" checksum="21D5F6B9D3E533EF" modified="2022-05-25" version="1">MPTRFGQVLAHGKTKLDVVYTNESREMPYFLKQLKERWLDAAMDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRSGITFAIVDITNDKLKMRTSMAHMAVALIDGEYGDMKTNFPKSQHKLWEKAPLDRINIEYAAKDAYVSYELYRKIRVINYGQRHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YT58</accession>
    <name>A0A8I6YT58_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123404046</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044953895.1">
        <property type="nucleotide sequence ID" value="XM_045097960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YT58"/>
    <dbReference type="SMR" id="A0A8I6YT58"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592780"/>
    </dbReference>
    <dbReference type="GeneID" id="123404046"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0491320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0491320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0491320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404046"/>
    <dbReference type="OMA" id="DNFNHFR"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22583">
        <property type="entry name" value="Rcat_RBR_ARI7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23141">
        <property type="entry name" value="RING-HC_ARI6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000005">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="127"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="131"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="525"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="589" mass="66105" checksum="DC13DE3D564EA8A7" modified="2022-05-25" version="1">MDSEDDARDSADEDFYSGGEAGLALSDDGDADYDFADRDSDDSDDLISHRQQQNYSVLSETDIKQHQADDMNRVSTVLSISKSEACALLRSYNWSVSKVHDEWFVDEARVRTAVGLPEKQNEMPNERELTCGICFESCPRESMGAASCGHPFCGVCWRGYIGTAINDGPGCLMLRCPDPSCSAAVGQDMVDLFASEEDKGKYERYLCRSYIEDNRKTKWCPAPGCEYAVEFVVGSGSYDVSCGCSYGFCWNCTEEAHRPVDCSTVSKWILKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYEAARQEGAFDDSERRREMAKNSLERYTHYYERWAANQSSRQKALGDLQSLQNDKLEKLSDIQSQPESQLKFIIEAWLQIVECRRVLKWTYAYGYYLPDHEHAKRQFFEYVQGEAESGLERLHQCAEKELQIYLDADCPSKDFNDFRTKLAGLTSVTRNYFENLVRALESGLNDVGAHSGQGACSKAVSSNSKNLGGKSKSSKNKAPGGASKSGSSTRGMDDGNIWACDQCTFANPRSARSCQVCDHQQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YU68</accession>
    <name>A0A8I6YU68_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YU68"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0580670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0580670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25839" checksum="C1ACEC2877671353" modified="2022-05-25" version="1">MSSCGYRWYPEYTLEEQHRDFNQSQYMCTVRVFPDYPGAEEPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDILLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVE2</accession>
    <name>A0A8I6YVE2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123398458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948863.1">
        <property type="nucleotide sequence ID" value="XM_045092928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVE2"/>
    <dbReference type="SMR" id="A0A8I6YVE2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0512400"/>
    </dbReference>
    <dbReference type="GeneID" id="123398458"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0425650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0425650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0425650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0512400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0512400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398458"/>
    <dbReference type="OrthoDB" id="602568at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF44">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035277875" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="42590" checksum="8DF067A6B8FF49F5" modified="2022-05-25" version="1">MARWPAAAALCALGAALLGMLAATADGNSKFTCTDTEKKRPGCTGTCPDRCPQNCISLCPSCQTYCPDQVQPVRPSLFVFGDGLTDVGNNNYLENNEVGNPLRANHSYYGIDFPNSVATGRFSNGFNMADFIAKAMGFEMSPPAYLSLNSPIKMEAGFAGVNYASATSGIWRDIDNGLDIPLMDQVKNFADTIGQMGANRSQQELSKMLSNSLFLIAIGTSDLYLIYNINNNGGSDNKTDVPHLISSYGDSLTALYNLGARKFGIINVPTLCEPMVRHGCEGLITSLREEFNDGIMPLMAGLASNLSGLRYSIADFGAFSDAVNTDPSAYGFVNTGGACCQGSCAPSSGLPCANRSQYWYWDEENPTEQAAKLAASAFFDGTTQFTMPVNLKTLISQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YYE1</accession>
    <name>A0A8I6YYE1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123411886</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960781.1">
        <property type="nucleotide sequence ID" value="XM_045104846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YYE1"/>
    <dbReference type="SMR" id="A0A8I6YYE1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0725070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0725070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0725070"/>
    </dbReference>
    <dbReference type="GeneID" id="123411886"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0601310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0601310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0601310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0725070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0725070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0725070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411886"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05472">
        <property type="entry name" value="cnd41_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000013">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000021">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033873">
        <property type="entry name" value="CND41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF902">
        <property type="entry name" value="OS06G0610800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035210084" description="Peptidase A1 domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="163"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0725070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="499" mass="51276" checksum="956575332FFD1BF7" modified="2022-05-25" version="1">MAASAAASPRHGFLGLLLAAAAVLAAFGLGAARAAGSSDDRALLSVASLFPGPACPATAEHGPSAAASARMRIVHQHGPCSPLADAHGKPPAHDEILAADQNRVESIQRRVSATTGRDKLTKHAAPVQPGPKKSPGIHPGHSASSSTPSLPATSGRAVSTGNYVVTVGLGTPASKYTVVFDTGSDTTWVQCRPCVVKCYKQKEPLFDPAKSSTYANVSCTDSACADLDTNGCTGGHCLYAVQYGDGSYTVGFFAQDTLTIAHDAIKGFRFGCGEKNSGLFGKTAGLMGLGRGKTSLTVQAYDKYGGAFAYCLPALTTGTGYLDFGPGSAGNNARLTPMLTDKGQTFYYVGMTGIRVGGQQVPVAESLFSTAGTLVDSGTVITRLPATAYTALSSAFDKVMLARGYKKAPGYSILDTCYDFTGLSDVELPTVSLVFQGGACLDVDVSGIVYAISEAQVCLAFASNGDDESVAIVGNTQQKTYGVLYDLGKKTVGFAPGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZB7</accession>
    <name>A0A8I6YZB7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123404796</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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        <location>
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</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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    </evidence>
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</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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        <location>
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        <location>
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        <location>
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            <end position="125"/>
        </location>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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</entry>
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            <fullName evidence="5">DUF569 domain-containing protein</fullName>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I6Z7H4"/>
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    </dbReference>
    <dbReference type="GeneID" id="123410058"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0656330.1">
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="DUF569" evidence="2">
        <location>
            <begin position="189"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32345" checksum="1450E0A5E28DFBDF" modified="2022-05-25" version="1">MDRFVDMQHVRLRNRVLDKYYLCADDDGEGVSISQHRNSLNAAWRVHIYHGADGPHLLLHSAAYGRYLSATGTPAPPFHHGFRAKQRDYDQPVVPSIMWLAVRAGLGGGILLENGGVRYLRANGKYLRWNTGVTVENRDIEKVSAMMRWTVEPILLRERPPRIPGPIRTRLPDDLSVTVFRRQPGAWQVIRFVQANPDGWFPEAHQDWSAFHFRGRSLYHLRDELARQVKQQPDGIAMCVRAGRYGRPTPLVVNLPRGGWGDTLQIIVVVSGTPAYEGLRYPNVG</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680700.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0680700.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0680700"/>
    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28990" checksum="13F43A7D0E92E602" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSEYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSREEGYLSGVYFDSSMGDPLLQSTVEMLENRDKDARALRMELYATRARLWTALMQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
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    <accession>A0A8I6ZED3</accession>
    <name>A0A8I6ZED3_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
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        <name type="common">Domesticated barley</name>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZED3"/>
    <dbReference type="SMR" id="A0A8I6ZED3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0733760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0733760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0733760"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0733760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0733760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3'-5'_exonuclease_dom"/>
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        <property type="entry name" value="3-5_Exonuclease_domain"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="80"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0733760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="28814" checksum="73E74EE4D89AC8C8" modified="2022-05-25" version="1">MKSTNRSTVAISLFLLLSHSPSLFFSSLLPFSSSTMPTSFHKVLAHGTTMLDVVYTNLSSEVPFFLQQLKEKWFDNAVDHEKFLGLDLEYTADQCGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSDITFASVDIRNDKLKMRHNFGIEIPAGCLVDLQTIFRLRHDRTSMAHMAVSLIDDSYADMKTSFPKSQHKLWKKGPLDDINIEYAAKDAYVSYELYRKIRVVHYGQRHLEEGGHSDLDDSDE</sequence>
</entry>
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    <accession>A0A8I7B1C0</accession>
    <name>A0A8I7B1C0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <location>
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            <end position="161"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0071240.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="20548" checksum="DFA96BBA7B7DFB5A" modified="2022-05-25" version="1">MVPSHQGKLTANQKEEVQPKDRVQRWTKPPPGWTKLNIDGDYSEGNKTGGAGMILRDEEGQVIFSSCRYLRTCSSALEAELMACLEGISIARVWSELPMIVELDCSCAVQMIVEEGIDRSPHATLVMQLKQALGEIGEHVISHVVRSRNALADALARLGRINHRTAVWLRGIPEEVDTLCKEDSVG</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
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            <begin position="49"/>
            <end position="151"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031770"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0109470.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="17250" checksum="4140A0A41BBD5315" modified="2022-05-25" version="1">MAQEQAHSSAVERLLNCEVPLRAQYIRVLFCEITRISNHSLASTTHAMDVGASTPFLWAFEEREKLLEFYERVPGARMHASFIRPGGVAQDLPLGLCRDIDSSTQQFASRIDELEEMSTGNPIWKQRLVDIGTVTAQQAKDWGFSGVMLRGHAT</sequence>
</entry>
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    <accession>A0A8I7B293</accession>
    <name>A0A8I7B293_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
        </source>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_GRF"/>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="74"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0012300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="14873" checksum="F807FE34E14ABF38" modified="2022-05-25" version="1">MSSSASASRRSWPRYGTVPMTRCPACPRTAPLKRVVTTTDKNGNLGREFVKCESKQEQGKKLKQCAHFEWLDEYIERIQLEGASRELDLPLEAEKFGSGPSGSGNSIGATVGDATGTAELKKLNKQMKKLIELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2F1</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Hordeinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
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                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I7B2F1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016530.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0016530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14435" checksum="132E165DDBB2E9AA" modified="2022-05-25" version="1">MVLISVPVGPRPVYDSDVEVVEPEMPIDPIGEKLHLVEQENVALKERLKKTEERLEKTEREKMTLEKVLAGVVDGHKSEMDAMRLKIRKIRKYAISKEAWYHYAVGSIITFVAILIAFVVVFKCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B385"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172820.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0172820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0142830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0142830.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0142830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0172820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0172820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0172820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="10557" checksum="9FD6ADB8E3634C15" modified="2022-05-25" version="1">MLLHVDPARRPRINRSVAIPSPEVDQAVFFLRNHAVTLSVADGVNASSPMAVGRALEAQLAVPAHSLRVTAHHPEHFFVIFTQPAHQVNAVRVGIMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3B0</accession>
    <name>A0A8I7B3B0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">68 kDa protein HP68</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123439324</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971993.1">
        <property type="nucleotide sequence ID" value="XM_045116058.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8I7B3B0"/>
    <dbReference type="SMR" id="A0A8I7B3B0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0057810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0057810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0057810"/>
    </dbReference>
    <dbReference type="GeneID" id="123439324"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0045760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0045760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0045760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0057810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0057810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0057810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439324"/>
    <dbReference type="OrthoDB" id="6593433at2759"/>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016887">
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        <property type="project" value="InterPro"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ribosomal small subunit binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
    <dbReference type="CDD" id="cd03237">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-binding cassette sub-family E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="67"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="340"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0057810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="67269" checksum="6C586F06990DEED9" modified="2022-05-25" version="1">MAARIAIINEDRCKPKKCRQECRKSCPVVKTGKHCIEVIPASKTAFISEELCIGCGICVKKCPFGAIQIINLPKDLDKDTTHRYGPNTFKLHRLPVPRPGQVLGLVGANGIGKSTALKVLSGKLKPNLGKFDNPPDWQEILTYFRGSELQNYFTRLLEDNLKAIIKRQDVESIPKAVQGNVGQLLDKLDQRGVKDQLCIDLELNQVLDRNVEDLSGGELQRFAIAGTAAQSAEIYMFDEPSVYLDVKQRLKAAQVIRSLLRANSYVIVVEHDLSVLDYMSDFICCSYGKPGAFGVVTLPFSVREGINVFLAGFVPTENLRFRDESLTFKIVEAQENAEEIETYQRYKYPTMSKTIGNFKLTVMEGEFTDSQIVVMLGENGTGKTTFIKMLAGRLKPDTVEGAEIEIPKFYVSHKPQQLETKFQGTVSQLLHQKIPESCAHPQFRSDVIKPLQIEQLMDRVVTNLSGGERQRVALCLCLGKPADIYLIDEPSASLDSEQRIVASKVIKRFILHAKKTAFVVEHDFIMATYLADKVIVYEGRPSVDCTANAPQTLLSGMNKFLSQLDITFRRDPTNYRPRINKLDSMKDKEQKSVGSYYYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5P6</accession>
    <name>A0A8I7B5P6_HORVV</name>
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        <recommendedName>
            <fullName evidence="10">NUC153 domain-containing protein</fullName>
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        <name evidence="8" type="primary">LOC123425834</name>
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    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the ESF1 family.</text>
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            <end position="433"/>
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            <end position="716"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004604"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139260.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="90602" checksum="5007C0417FE6F007" modified="2022-05-25" version="1">MAPTADSVDLKGQKKVKKSNSKDERKHRKDKHERPAADGASLRDDAKRKHKGGQEGKEHGKKPKKEKKAGAEAEAMVAGAAEAKRGDEKMKRAMEDERFAAARTDPRFRPMRRREAKVALDSRFNGMLTNPMFASSEAPVDKRGRRRKKGAKENPMLHYYLKQEEGDEKEKEKEKVKLVQEDEDDEVEEEDEQVEQESSSSDDEEEEEDDDDDECSVGSDIAHYLMARHDDTPMIDKETHRLAVVNMDWDHIKAVDLYMVMTSCIPKGGRVLSVSIYPSEFGLKCMNIETTQGPSILLGIDGDSEGDGGEDGNDSDSDEEDDKEDREQDSEDDNENDNDNDSDEDDGNDSDEDDNDEEDDEHDSDSENNKLRTYELNRLRYYYAVVVCDSSTTANHLYTTLDGTEFLKTANVFDLQFISDNMEFKHAARDVATEAPPNYKEPDYETRALQHSKVKLTWDDDEPERKKKLRRKFNDEQLDDLGVFLASDDSESDDDVADSGDEPRPNGVPKRKLTHKERMALLLEGDKSDEEQADDQDMEVTFNTELEDLSKRILERKSSEKKTVWEMHQEKMKEKRKARKRSSKDDDDDDDSNEDGADEDEDFFEDEKSDEDVKPMKKQKVKAKDKEKGKGKDKLPEEHFEPEATKEELELLVADQDAGNGAKGYNIKRKSKKGKKGKDSVEDKHPHIDLTNDKRFDPMFTSHLFALDPTDPQYKRSADFKRKQTGKKGVRAGTLDSEPPVEESSPGSTLPADDASTRKTKQKHDGASTEKLQMLSAVKSLKRNLTAFKKGSSTSER</sequence>
</entry>
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            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="7">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.5) subfamily.</text>
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            <end position="134"/>
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            <begin position="272"/>
            <end position="383"/>
        </location>
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        <source>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049662"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B955</accession>
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        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
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        <name evidence="5" type="primary">LOC123442797</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974878.1">
        <property type="nucleotide sequence ID" value="XM_045118943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B955"/>
    <dbReference type="SMR" id="A0A8I7B955"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233700"/>
    </dbReference>
    <dbReference type="GeneID" id="123442797"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193960.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442797"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF24">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="478"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="513"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="62202" checksum="50E98225F598CF5A" modified="2022-05-25" version="1">MNAAKSGGRMLARRVVPPPCRGAASAVPAATSTPQHISHYLAHSPRVTWEALSASFPAAPAPEGHVDAVLLSLARNPSPSSPASAETVAKNAHSFFHWSAAASPSPHSLRSYCLLVHLLARAALISHASVLLQAAITRHSSSPASSFLDAFFSAYEDSGTAATTRGLHLLVHAYAQLRLPEEALEACRYLALRRVLPSLSAFNAVLHAAQRTGRFRVAWEVFELMTLKRVYASQATVELVVGVLSREGTLARTVALVERIHGKKCAPGVVAHVALALWIFEEGRAEKGILLLRRMLQRNMVFDDVAYSLMVHAHCRIGDLESAREQWDDMVRRGCRLNPFVYTCLVGVHCREGNVSEAMQLLQEMLSRGLKPYDATYSHLVTGCFRHERTGEGLEYFDKMLHEGLVPDIGTCNEILEAMCGAGEVGKANELVTAMIDKGIIPGQDTYHKLIDGFGKVGDVEGVVKIYYEMEHRGLNHGSDFFTSLISALCQCGNPKEAEKFVYVMERKSLALTSDISDMLISSYCKKGNTKRALRLYDKMITRSEKLIPSADTFMMLVRRVIKVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9C0</accession>
    <name>A0A8I7B9C0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LTV1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123399217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949580.1">
        <property type="nucleotide sequence ID" value="XM_045093645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9C0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489690"/>
    </dbReference>
    <dbReference type="GeneID" id="123399217"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0405750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0405750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0405750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0489690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399217"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="407"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54035" checksum="7F8EDE56460A8727" modified="2022-05-25" version="1">MAGAGVGGRKARNFATFRLFPRDGAADPNDRVFVRVDNNAYTIPGFGDDEDPSLSPTAAAADQLPSSTSGPLPDHVRQQILELGLPDDGYNYLLHLRELRPSAVPSSFVPNHTTRPEHVPLDVKAYDASKVRVASGTVEEELDEGRTMCKVAAKTAPVRRVEKAVDPDVARLLDESEDEGLEEDFVIMANQAEGDDMEDESEEEGGGVFSDVENDEEFEVEEGEPKPRVPRLLDEQFDLLALEEYGASDDDDGAVRDGEHELPTEVIDELKLFHNQNVCVDEEYRTPADFVRGKLDSTTAEEVDESANVIQKCAEYAERYLNENAEDEEVLLVSESSDESEVWDCETIVSTYSNLDNHPGKIQTPGNPRNRLPKVFPGETATTKDIIKLHGKEKLPVDYLPQRKRKVEKEKKAKPTEEASDAEYFKKVVQKETKDEKKARKSAVKEEKREARKAKKELKELYKFETQKAQKVAAITGPSSIRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9S8</accession>
    <name>A0A8I7B9S8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123452145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984674.1">
        <property type="nucleotide sequence ID" value="XM_045128739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9S8"/>
    <dbReference type="SMR" id="A0A8I7B9S8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509130.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509130.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509130"/>
    </dbReference>
    <dbReference type="GeneID" id="123452145"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0422830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0422830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0422830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509130.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509130.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452145"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000504">
        <property type="entry name" value="F-box family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000597">
        <property type="entry name" value="F-box/LRR-repeat protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF45">
        <property type="entry name" value="OS03G0633400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509130.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="60598" checksum="EFB69ABB2B5CB6C1" modified="2022-05-25" version="1">MSSSSLPTKPPPPPRPKTRGSYNCGRCGQPKKGHVCNLPSPSPAPAAADAPSPSSSAGESRLRRALSFDDAPSSPEKKPRPDDDDGRTMDLGGRPVPVDFFLEVLRRLGPRGVLDAAAVSRAWRDCADRVWRVAEELRLRPAGAGLLAALLPRCAALSRLVLHMESDVDATMLSCLAFSCSSLEILEITMSDKASNNMTGEELSRFVSEKHSLSILKIGGCSNLGFLNLSSSSLNVLWLSDLYSLSESVISCPNMSELSLCFTQQSTDCTDLVSLMDGLGRSCPNLRNLHISSIQLSNEAVSALEGANLRGLCMLSLILGSKMTDAAVASIVRSCASLALLDLSGSSISDSGVGMIGKAFPHTLSRLLVALCPNITTSGIQAATVQLPLLQLMDCGMSLRSNSQNEKQGAYFGEINGRIRLCPKLPTLKKQPMRQKLIIKHDNLKKLSLWGCSAIDALYVKCPELIDLNLNSCTNLHAERLLLQCPNLKNVHAFGCQDMLIGAIRNQVLNEFAAAEPHLPCKRLADGSKRVQLSQFPQEQLPEDKIWIGFKRSECIVHLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAE1</accession>
    <name>A0A8I7BAE1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123399367</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949718.1">
        <property type="nucleotide sequence ID" value="XM_045093783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAE1"/>
    <dbReference type="SMR" id="A0A8I7BAE1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529590.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529590"/>
    </dbReference>
    <dbReference type="GeneID" id="123399367"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0440540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0440540.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0440540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0529590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529590.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399367"/>
    <dbReference type="OrthoDB" id="1893323at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF439">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="393"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529590.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="46877" checksum="7E90B536FC8CA0E5" modified="2022-05-25" version="1">MARCSLAKPLGTAAAAPHLPRRRALLASAAAASLATATTRTQRLSALSKPPKPPPPPPLRPRPRLPISNDTPSNAKQDGRREPSTDATESSVSGSSSSGAGDVLRLMDLLGVVPDEDVYVSLLRDSVDAAEVAAVHSHVVSTRAAPGLPLPLANRLLLAFATCGDMVAARKVFDEIPVKDGITWATMVSAYSDGCFHNEAIRLFTRMCHEEQGLTGDLLGHAIVAVLRSCARMSTLRGFGEQVHALLVKTKRVCGDTGSSLLQLYCAGKRHDIARQVLQTMKCSWQEPVPEAAWTSFITACHRDGGLDEAIYVFSDMVSSGVARSSFSLSTILAVCAESENCRFYGQQLHGDAIKHGVETDQFVVSGLVHMYARQGHLADAARAFEAVGGKPDVVCWNAMALGYARGGWYREATSMMYQMKAAGMDLPGLNVVGMACSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BC72</accession>
    <name>A0A8I7BC72_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC72"/>
    <dbReference type="SMR" id="A0A8I7BC72"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0374550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0374550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0374550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0374550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0374550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0374550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0374550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24785" checksum="3293FDE71FBBCB0F" modified="2022-05-25" version="1">MPHLYDLYGMAHTASYKKAKAFSESNLDDPSNFTNISSHQKLVAYRDAGKATKGDDFNPTQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKARQSSRCPKITPRPRPVELAIEAALQKERAANQAALEKERLASQAALQAALDERDHTTARLLEEERARNEAGQRALYELFVGLCEKSGQVPPPMPVFSSIGTNNSRVASHDPSPGQPSPNEAGASNRGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCM1</accession>
    <name>A0A8I7BCM1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953134.1">
        <property type="nucleotide sequence ID" value="XM_045097199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCM1"/>
    <dbReference type="SMR" id="A0A8I7BCM1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624440"/>
    </dbReference>
    <dbReference type="GeneID" id="123403256"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0518210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0518210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0518210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0624440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403256"/>
    <dbReference type="OMA" id="NCIRRDE"/>
    <dbReference type="OrthoDB" id="784883at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025521">
        <property type="entry name" value="Neprosin_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF135">
        <property type="entry name" value="OS05G0341100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14365">
        <property type="entry name" value="Neprosin_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144110" description="Neprosin PEP catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="2">
        <location>
            <begin position="131"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="385" mass="42627" checksum="0A816FC2FA06CDB7" modified="2022-05-25" version="1">MIENPSISVTILLACIILALGGNEVKCTRRDENASGVPWNQKVNNTIQVDVGDVYDCIDVNLQPAFSHPLLKHHKIQMEPSSLPLSISTKFPSIHAMPPTQLSLIECPTGTIPIVRNNKRVHKLAQAIDKVIRKDEQQEVAGFEYLDVLYGTRAKINIYEPKVKNNSKDLSASWIQIKGTQKVGRADGIGAGSWVYPSYSGDNLARFHVAWVDGLKNTICPDHDCGAFIQVSSSVGLGGRLKPVSIYNGPQYIIDVTIFKDPLTKNWWVSYGEQNIHIGYWPREIFHFMKDQCNYAFWGGYVQGPTASSHSPQMGSGHFASEERGKAAVIRNILVVDKENMYATPDARKARLVTTSSSKYTAKAYGDGYNDYGVHTYYGGPGAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEL0</accession>
    <name>A0A8I7BEL0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="3">1.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123410462</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044959343.1">
        <property type="nucleotide sequence ID" value="XM_045103408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEL0"/>
    <dbReference type="SMR" id="A0A8I7BEL0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701760"/>
    </dbReference>
    <dbReference type="GeneID" id="123410462"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0582100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0582100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0582100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0701760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410462"/>
    <dbReference type="OMA" id="GMFELEQ"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="6">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="21707" checksum="211191CD31E8B99B" modified="2022-05-25" version="1">MAAKIYIVYYSTYGHVAKLADEIQKGVSSVEGAEVKLWQVPETLSDEALAKMGAPPKRDDVPVISPAELADADGLIFGFPTRFGMMPMQFKAFMDGTSELWCPQRLAGKPAALFFSSGCQGGGQETTALTAITQLVHHGMLFVPVGYTFGSGMFEMGQVKGGSPYGSGTIAGDGSRVPTELELQQAFHQGKYFAGIAKKLKGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFY6</accession>
    <name>A0A8I7BFY6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFY6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0444280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0444280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0444280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0534080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019275">
        <property type="entry name" value="DUF2301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36716">
        <property type="entry name" value="F3H9.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36716:SF2">
        <property type="entry name" value="F3H9.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10063">
        <property type="entry name" value="DUF2301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="214" mass="22225" checksum="575129B4FCFBA38C" modified="2022-05-25" version="1">MLRAIAFLSACPEVFSARSSTTTALLRLAPLPPHPSLVAPKKPSLLAPLGSGRRATGALQLARAAGDGLADQTTVYNCVYGADVREGADLFYAAGAGGLGLSLVLIHIYVTPIKRFLQALWDVGVLGSVGTYALAARPLDEGLVRYVAMCSSTLTRCGSSGRPSPRSLASSSRKVSAMGNWKLVSSFAIPILLLGHLSGFMDDGVKLSLLALGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGZ6</accession>
    <name>A0A8I7BGZ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGZ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0588430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0588430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0588430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0588430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0588430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0588430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="AntiFam" id="ANF00025">
        <property type="entry name" value="Antisense to 23S rRNA"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0588430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="82" mass="9609" checksum="2820E0D905156551" modified="2022-05-25" version="1">MSISPSLSLRQCPDRYAFHAGRNLPDKEFRYLRTVIVTTTLHRGFGRRLPCHQFTNFLDLPALGRRQPPYMVLRLSGDLCFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F2CPT0</accession>
    <name>F2CPT0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123437326</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Seed</tissue>
            <tissue evidence="8">Shoot</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK353632">
        <property type="protein sequence ID" value="BAJ84851.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK363246">
        <property type="protein sequence ID" value="BAJ94450.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK377169">
        <property type="protein sequence ID" value="BAK08363.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971636.1">
        <property type="nucleotide sequence ID" value="XM_045115701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CPT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0054390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0054390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0054390"/>
    </dbReference>
    <dbReference type="GeneID" id="123437326"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0042700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0042700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0042700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0054390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0054390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0054390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123437326"/>
    <dbReference type="OrthoDB" id="19798at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF3">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="7">
        <location>
            <begin position="25"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ84851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ94450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK08363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0054390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="295" mass="33863" checksum="8DBFB922F73876F9" modified="2011-05-31" version="1">MGRLFAVDAAAASSAVAAAAMTGAVDWWKDVNDSPMWQDRIFHALAVLYGIVSVVALVQLIRIECRVPEYGWTTQKVFHFLNFVVNGVRSIVFVLRRNVQLIQPEILQHVILDMPGLAFFTTYALLVLFWAEIYYQARAMSTDGLRPTFYWINGVVYAIQIILWLVLWWKPVRVMVILSKMFFAGVSLFAAFGFLLYGGRLFLMLQRFPVESKGRRKKLQEVGYVTTICFTCFLIRCVMMCLNAFDKAADLDVLSHPILNFFYYLLVEIIPSAMVLFILRKLPPKRGITQYHPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2CVS1</accession>
    <name>F2CVS1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123426522</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK355724">
        <property type="protein sequence ID" value="BAJ86942.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966297.1">
        <property type="nucleotide sequence ID" value="XM_045110362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CVS1"/>
    <dbReference type="SMR" id="F2CVS1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0161860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0161860"/>
    </dbReference>
    <dbReference type="GeneID" id="123426522"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0133550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0133550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0133550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0161860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0161860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0161860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426522"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098572">
        <property type="term" value="C:stromal side of plastid thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05243">
        <property type="entry name" value="SDR_a5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000408">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF48">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="83"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ86942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="327" mass="35174" checksum="6188B9752F5EA00A" modified="2011-05-31" version="1">MATAHLSPALAVSSSRHLTCKHGKPPVFAQPGGTVVLRSCSLPLSSRLAASGRGGWRLAAAVEPMTAQQEKAEASSRLVLVVGGTGGVGQLVVASLLSREIKTRLLLRNPGKAASLFGKQDESVLQVYEGDTRNADDLNPEMFEGVTHVICTTGTTAFPSKRWDGDNTPERVDWDGVRNFVSAMPQTIKRLVLVSSIGVTKYNEIPWSIMNLFGVLKYKKMAEDFVRNSGIPFTIIRPGRLTDGPYTSYDLNTLVKATAGERRAVEIGQGDKLVGEASRLVVAEACIQALDIESTQGKIYEISSVKGEGPGSDQEKWKQLFAAAESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0Z5</accession>
    <name>F2D0Z5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357552">
        <property type="protein sequence ID" value="BAJ88766.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0Z5"/>
    <dbReference type="SMR" id="F2D0Z5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0485540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D0Z5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="12"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="187"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ88766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="310" mass="34022" checksum="846C6FAD775E4FE8" modified="2011-05-31" version="1">MRAMARIDEWKPVIAMLVFDLISAVTTALIKAALEEGLDRLVLITLRQLVATVFLSPIAFFKERNTRPKLTLEILVYLFFSAALGAALSQYTFFYGLQYTTATFAITFTNLAPVLTFLIAVLLRVESLNMKNKAGAAKIIGTLMSFAGVMLLTLYKGVALTHQAEPSGQHAEAAAAAAESGKKSWTLGTLALLANCLCFSFWLLLQSRLTKKYPALYSSTAYMFVISSLQGGGLTAAIQRRASVWVLTKPVEIVTVLYTGILGSGVGYVLMTWCVEKRGPVFTSSFIPIIQIMVAIIDFFFLHENIYLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2D4J2</accession>
    <name>F2D4J2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123447946</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK358802">
        <property type="protein sequence ID" value="BAJ90013.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980623.1">
        <property type="nucleotide sequence ID" value="XM_045124688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D4J2"/>
    <dbReference type="SMR" id="F2D4J2"/>
    <dbReference type="STRING" id="112509.F2D4J2"/>
    <dbReference type="PaxDb" id="4513-MLOC_78379.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0348030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0348030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0348030"/>
    </dbReference>
    <dbReference type="GeneID" id="123447946"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0290380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0290380.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0290380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0348030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0348030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0348030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447946"/>
    <dbReference type="eggNOG" id="ENOG502RXST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087111_2_1_1"/>
    <dbReference type="OMA" id="FTTFKLY"/>
    <dbReference type="OrthoDB" id="1864232at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019593" description="Dirigent protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ90013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0348030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="181" mass="18786" checksum="450D0E83AD1A5CF3" modified="2011-05-31" version="1">MAGSKAVLLLLLCAAALLTPASAADDGLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGPAVTGSAKSKDEIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGKFRMARGYVEARTMDSGAKSGETVVEYTVYVKAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5D8</accession>
    <name>F2D5D8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK359098">
        <property type="protein sequence ID" value="BAJ90309.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5D8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0050020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0050020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0050020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0039060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0039060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0039060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0050020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0050020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0050020"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043302945" evidence="2">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ90309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0050020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="98" mass="10417" checksum="893E4AE9F8CDB6ED" modified="2011-05-31" version="1">MRLFFKLFAVLAIMAALSATNVPSAVGVWARTPGRAPPSPTPSALRGRVKPINPAPSPPPPAEMGRVRPLRSPLPPPSPNRQRSVGSTQFPPPPSRCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DA40</accession>
    <name>F2DA40_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123406116</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="AK360753">
        <property type="protein sequence ID" value="BAJ91961.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955537.1">
        <property type="nucleotide sequence ID" value="XM_045099602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DA40"/>
    <dbReference type="SMR" id="F2DA40"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557340"/>
    </dbReference>
    <dbReference type="GeneID" id="123406116"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0463020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0463020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0463020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0557340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406116"/>
    <dbReference type="eggNOG" id="KOG0497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="602388at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DA40">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016871">
        <property type="term" value="F:cycloartenol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000002">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000022">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="99"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="414"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ91961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="760" mass="86203" checksum="7FD3C7F316413600" modified="2011-05-31" version="1">MWRLKIAEGGADPWLRTKNAHVGRQVWEFDAAADDPDALAAVDAARREFAARRHRLKHSADLPMRIQLAKENPLKLDLPAIKLGENEDVTEEAVSTSLRRAISSFSTLQAHDGHWPGDYGGPMFLMPGLLIMLHVTGALNTVLSSEHQKEIRRYLYNHQNEDGGWGLHIEGPSTMFGSALTYVSLRLLGEGPDSGDGAMEKGRNWILDHGGATYITSWGKFWLAVLGVFDWSGNNPLPPELWMLPYCLPVHPGRMWCHCRMVYLPMCYVYGKRFVGRITPIILELRNELFKVPYSEVDWNSARNLCAKEDLYYPHPLIQDILWATLHKFVEPVMMHWPGNKLREKSLNHVMQHVHYEDENTRYICIGPVNKVLNMLACWIEDPNSEAFKLHIPRIYDYLWFAEDGMKMQGYNGSQLWDTAFAVQAIAATDLIEEFAPTLKLAHDYIKNSQVVDDCPGDLSYWYRHISKGAWPFSTTDHGWPISDCTAEGLKASLLLSKISPEIVGEPVEVNRLYDAVNCLMSWMNENGGFATYELQRSYAWLELVNPAETFGDIVIDYPYVECTSAAIQALTSFKKLYPGHRRKDVDNCINKAANYIESIQRSDGSWYGSWAVCFTYGTWFGVKGLVAAGRTFKNSPAIRKACDFLMSKELPFGGWGESYLSSQDQVYTNLEGKHTHAVNTGWAMLTLIDAGQAERDPTPLHRAAKVLINLQSEDGEFPQQEIMGVFNKNCMISYSQYRNIFPIWALGEYRCRVLGVGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJ04</accession>
    <name>F2DJ04_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123448515</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK363872">
        <property type="protein sequence ID" value="BAJ95075.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981364.1">
        <property type="nucleotide sequence ID" value="XM_045125429.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981365.1">
        <property type="nucleotide sequence ID" value="XM_045125430.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981366.1">
        <property type="nucleotide sequence ID" value="XM_045125431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJ04"/>
    <dbReference type="SMR" id="F2DJ04"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0368950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0368950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0368950"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0368950.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0368950.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0368950"/>
    </dbReference>
    <dbReference type="GeneID" id="123448515"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0307130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0307130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0307130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0368950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0368950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0368950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0368950.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0368950.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0368950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448515"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009704">
        <property type="term" value="P:de-etiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080167">
        <property type="term" value="P:response to karrikin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000042">
        <property type="entry name" value="probable strigolactone esterase DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43039">
        <property type="entry name" value="ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="21"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ95075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0368950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="271" mass="29753" checksum="9D25B6872870D948" modified="2011-05-31" version="1">MGIVEEAHNLRVVGEGKRGVIVLAHGFGTDQSVWKHLVPHLVADYRVVLFDTMGAGPTNPDYFDFSRYATLEGYALDLLAILEELGIASCIYVGHSVSAVIGVLASISRPDLFSKLVLLSASPRYLNDVDYYGGFEQEELDELFEAMRSNYKAWCSGFAPLCVGGDLESVSVQEFSRTLFNIRPDIALSVAQTIFQSDVRTLLPLVSVPCHIVQSTKDLAVPVVVSEYLHKHLGSDSIVEVMPSEGHLPQLSSPDIVTPVLLRHIQHDIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJM7</accession>
    <name>F2DJM7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448666</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="AK364095">
        <property type="protein sequence ID" value="BAJ95298.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981564.1">
        <property type="nucleotide sequence ID" value="XM_045125629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJM7"/>
    <dbReference type="SMR" id="F2DJM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0377080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0377080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0377080"/>
    </dbReference>
    <dbReference type="GeneID" id="123448666"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0313810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0313810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0313810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0377080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0377080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0377080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448666"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22153">
        <property type="entry name" value="F-box_AtTLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000003">
        <property type="entry name" value="Tubby-like F-box protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF158">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="2">
        <location>
            <begin position="112"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ95298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0377080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="400" mass="44364" checksum="A356EA590F236578" modified="2011-05-31" version="1">MSFRSLIQDMKDEFGNISRHGLRSSRSHRVAAGAPRVVSAGLADALEGTCWAQLPPELLREVLVRIENSQGCWPSRRDVVACAGVCRAWRGIMKEVVRVPEASAKLTFPISLKQPGPKDGTLKCFIRRNRTTQTYYLYTGLTEALADNGKFLLAARKCRKPTYTDYLIYLDMAGISKGSSTYIGKLRSNFLGTKFTVYDAHPPYDGAAVSKRRSSHVIGLNQVSPRVPAGNHPVSHISYELNVLGSRGPRRMSCVMSSIPASAVEEGGKAPTQTEFPLSNLDSFPSIPFFRSKSARIDSAPAQAQDEEKLVLKNKSPRWHEQLQCWCLNFRGRVTVASVKNFQLVASDDNGPVSQEHDKVILQFGKIGKDLFTMDYRYPISAFQSFAICLSSFDTKIACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLK1</accession>
    <name>F2DLK1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123399896</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK364769">
        <property type="protein sequence ID" value="BAJ95972.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950215.1">
        <property type="nucleotide sequence ID" value="XM_045094280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DLK1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495940"/>
    </dbReference>
    <dbReference type="GeneID" id="123399896"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0411310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0411310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0411310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0495940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399896"/>
    <dbReference type="HOGENOM" id="CLU_008321_3_0_1"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ95972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="635" mass="71927" checksum="9F7392E5BA2E641E" modified="2011-05-31" version="1">MSGRLVRRVLQEREASPQDTAAEEGLVVVVEEEEDDEEAASPTRVAARNPFDLLDDDDDEPEEEKEDEAHTDQTVSYTEQKQSVKKKPNGAVPETNKKSKKKKKKGKAEPPVSTKSRVEKSLDSILEDLAIEKKPMQKSVHQNDRATGKEIETNETAVGTSSVIAIDPKHLKAENEMRRIFGSKVVDSLENQRNMPGTSRQVRGVRRAALNPRRTLLVSPPGFWPPWDKSMSMNLVETKGSLNYFRYEYDPSVSHVQELFEAAKSANDLNAIAAILAKYPYHPDSLLTFADLFKYSGEHQSSADAVEKCLFALECAWHPLFNPLQGNYQLKYGHDINKPFFTALFSHMKNLDRRGCHRSALEACKFLLSLDSDDPKGALFCIDYFALRSHQHKWLEQFAEEYQCDNSLWLFPSFSFSLAIARFYLERDAASEDVSDHADKSTAIDLMKQALMLHPLVLSKIVDKAPLKDSSWTQILKNVFFGSAKPGSPSLEHVINIYVERHYIMWRFPELQNLLKEAALLVIESLKQDSREAQDWACVRKEAFSSEKNEYSHLLVSDFSDTTPSLPPEELRPFMVGPGMGHDMPPVEQEAGPERFRAPREVAGRNAALVFLESLLPWVDYGDNHHDGNDQDNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMT1</accession>
    <name>F2DMT1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-[acyl-carrier-protein] hydrolase</fullName>
            <ecNumber evidence="11">3.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123399754</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an essential role in chain termination during de novo fatty acid synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Plastid</location>
            <location evidence="1 11">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the acyl-ACP thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-" evidence="11"/>
    <dbReference type="EMBL" id="AK365199">
        <property type="protein sequence ID" value="BAJ96402.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950070.1">
        <property type="nucleotide sequence ID" value="XM_045094135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMT1"/>
    <dbReference type="SMR" id="F2DMT1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493850"/>
    </dbReference>
    <dbReference type="GeneID" id="123399754"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0409280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0409280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0409280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0493850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399754"/>
    <dbReference type="OrthoDB" id="618395at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000014">
        <property type="entry name" value="Acyl-[acyl-carrier-protein] hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049427">
        <property type="entry name" value="Acyl-ACP_TE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002864">
        <property type="entry name" value="Acyl-ACP_thioesterase_NHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045023">
        <property type="entry name" value="FATA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727">
        <property type="entry name" value="OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727:SF6">
        <property type="entry name" value="OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01643">
        <property type="entry name" value="Acyl-ACP_TE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20791">
        <property type="entry name" value="Acyl-ACP_TE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-ACP thioesterase N-terminal hotdog" evidence="12">
        <location>
            <begin position="73"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-ACP thioesterase-like C-terminal" evidence="13">
        <location>
            <begin position="239"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ96402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="354" mass="39453" checksum="544A98D8E7BBCCAC" modified="2011-05-31" version="1">MLRCHIPPQCGPAPLGRRGLAAATALRCAARGPSRGALVAAGAAVAAAVATAEGVERPGLAERLRMGSLLEDGLSYKESFIVRCYEVGINKTATVETIANLLQEVGCNHAQSVGFSTDGFATTTTMRELGLIWVTNRMHIEIYKYPAWGDVVEIETWCQADGKIGTRRDWILKDLANGEVIGRATSKWVMMNQNTRRLQRVSDEVRDEVFIHCPKSPRLAFPEENNGSLKKIPVLTDPAQHSRLGLVPRRADLDMNQHVNNVTYIGWVLESIPQDIIDTHELQTITLDYRRECQHDDIVDSLTYIEEGEEINSNGSLYSVPHPEEQRQFLHCLRFAGAGDEINRGRTVWRKLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2E4A5</accession>
    <name>F2E4A5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123425503</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK370979">
        <property type="protein sequence ID" value="BAK02177.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965132.1">
        <property type="nucleotide sequence ID" value="XM_045109197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E4A5"/>
    <dbReference type="SMR" id="F2E4A5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130150"/>
    </dbReference>
    <dbReference type="GeneID" id="123425503"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0107410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0107410.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0107410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0130150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425503"/>
    <dbReference type="HOGENOM" id="CLU_011924_5_1_1"/>
    <dbReference type="OrthoDB" id="1913536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008356">
        <property type="term" value="P:asymmetric cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009956">
        <property type="term" value="P:radial pattern formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="11"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="100"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="515"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="309"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK02177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="597" mass="64207" checksum="EC21AF55177281DF" modified="2011-05-31" version="1">MDTLFRLVSLQASEQQQQQQSASYNSRSTTSSGSRSSSHQTNASYNYYHHSTSSGGGGGGGGGGQYYYSQQQPQQSYYLEPYQEECGGNAHQHHLYMDEDFSSSSSSRQHFHSHGGAAGQPPTSSATPTAPTPPLSTSSTAGGAGHALFEAADLSFPPDLNLDFSSPASSSGAGGTASSGAVGGGGGGRWASQLLMECARAVATRESQRVQQLMWMLNELASPYGDVEQKLASYFLQGLFARLTASGPRTLRTLAAATDRNTSFDSTRRTALRFQELSPWSSFGHVAANGAILESFLEAAAASSEPQRFHILDLSNTFCTQWPTLLEALATRSPDDTPHLSITTVVSAAPSAPTSAVQRVMREIGQRMEKFARLMGVPFRFRAVHHSGDLAELDLDSLDLREGGANTGIAVNCMNSLRGVVPGGARRRGVFAASLRRLEPRIVTVVEEEADLVATDPDASDEGCDTEAAFLKVFGEGLRFFSAYMDSLEESFPKTSNERLALERGAGRAIVDLVSCPASESMERRETAAAWARRLRSAGFSPVPFSEDVADDVRSLLRRYREGWSMREASTDESAAGAGVFLAWKEQPLVWASAWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F2E6G5</accession>
    <name>F2E6G5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123407040</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK371739">
        <property type="protein sequence ID" value="BAK02937.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956009.1">
        <property type="nucleotide sequence ID" value="XM_045100074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E6G5"/>
    <dbReference type="SMR" id="F2E6G5"/>
    <dbReference type="STRING" id="112509.F2E6G5"/>
    <dbReference type="PaxDb" id="4513-MLOC_60881.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0741190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0741190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0741190"/>
    </dbReference>
    <dbReference type="GeneID" id="123407040"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0614750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0614750.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0614750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0741190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0741190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0741190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407040"/>
    <dbReference type="eggNOG" id="KOG0265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_2_1"/>
    <dbReference type="OMA" id="IWDIRPY"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E6G5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000229">
        <property type="entry name" value="Small nuclear ribonucleoprotein U5 subunit 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="313"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK02937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0741190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="38421" checksum="3F40EA461A49BE43" modified="2011-05-31" version="1">MYSAPGNNSLALAAPRPGMELGNVQQHPNQAFGLGPGGKQRSSSLEAPIMLLTGHQSAVYCMKFNPAGTVIASGSHDKDIFLWYVHGECKNYMVLRGHKNAILDLHWTTDGSQIISASPDKTLRVWDVETGKQVKKMAEHASFVNSCCPSRKWPPLVVSGSDDGTAKLWDLRQRGAIQTLPDKFQITAVSFSEAADKVFTGGLDNDVKWWDLRKNEVTECLKGHQDMITGMQLSPDGSYLLTNAMDNELKIWDLRPYAPENRNIKTFTGHQHNFEKTLLKCSWSPDNRKVTAGSADRMVYIWDTTSRRILYKLPGHNGSVNETAFHPTEPIIGSCGSDKQIYLGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2E9W5</accession>
    <name>F2E9W5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123408061</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK372940">
        <property type="protein sequence ID" value="BAK04137.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957205.1">
        <property type="nucleotide sequence ID" value="XM_045101270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E9W5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027800"/>
    </dbReference>
    <dbReference type="GeneID" id="123408061"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408061"/>
    <dbReference type="HOGENOM" id="CLU_518195_0_0_1"/>
    <dbReference type="OrthoDB" id="5395350at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041686">
        <property type="entry name" value="Znf-CCCH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725:SF22">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15663">
        <property type="entry name" value="zf-CCCH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK04137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="500" mass="56553" checksum="B6A10EE04866C60D" modified="2011-05-31" version="1">MVSAAAEEGEDPRWRRSNTDCVFFLVSRVSCTKGSKCEYRHCEGARFNPRNCWYWFHGSCVNPSCTFRHPPMENFNRTKSLTVPPSSDGSVSVKTANPCYFYYSSGCKKGDNCPFLHEPIPNTESAISSEATTFNPAVNENFSGDEMVEASEEAHVSPCQDTLYHIKKCHPKEVPESIYLEFDGPISVTPETSIDMGEYIKCFTHSDQSSEYSTMEDAEQDGSRDSSPGFDVLVDDGLSNKNDLEKQLAQKRDAQVLHAKYDIGDPACYDRDYYDSWYYGQAFCSFDDQHAYLSHPEGVQDPDVETTLGHILHNTRDLARPSFDEFDRMFFNSSFISSAADDVFPHQHNETRHTSKRRPEKRKGAKSRKGRTKKRRGLEPVCGFQGIESRSTHYRQESLMEECPESVVRATFRGQKKKRRGKQHNVISARFSEHPTTDFTGPKTLAQIKEDNCISKSRLSHSAARMPHGGTFSNDFEGPKSLTELMKAKGGISISQRTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2EH15</accession>
    <name>F2EH15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Origin of replication complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123444129</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ORC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375442">
        <property type="protein sequence ID" value="BAK06637.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976683.1">
        <property type="nucleotide sequence ID" value="XM_045120748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EH15"/>
    <dbReference type="SMR" id="F2EH15"/>
    <dbReference type="PaxDb" id="4513-MLOC_70691.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280730"/>
    </dbReference>
    <dbReference type="GeneID" id="123444129"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0232680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0232680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0232680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0280730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0280730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0280730"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007115_0_1_1"/>
    <dbReference type="OMA" id="AFTFQRN"/>
    <dbReference type="OrthoDB" id="343623at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EH15">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001041">
        <property type="entry name" value="Origin of replication complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016527">
        <property type="entry name" value="ORC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032705">
        <property type="entry name" value="ORC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14629">
        <property type="entry name" value="ORC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007858">
        <property type="entry name" value="ORC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="11">
        <location>
            <begin position="58"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 4 C-terminal" evidence="12">
        <location>
            <begin position="219"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR007858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK06637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0280730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="424" mass="48001" checksum="4FE1B52896A42766" modified="2011-05-31" version="1">MAAAAPAPVAGHAHAVLRGRLCDPAFVHSCLRSSPDTNYSKLKYLVASSVSEACNNSVLLLGPRGCGKGAVLDMVLEDLRKEHPDAISVIRLNGMLHSDDNCATKEIARQLCLEHQLSFSKMASSDDNTEFMIDMLRECGLAHKTVIFVLEEFDLFAQGKQRLLYSLLDAMQSLTSQAVVIGMSCRLDADQLLEKRVRSRFSHRKLLFVPSSLDDIQRLMEHLLMLEKDSSLPTNYVTEYNSRLTSIFSNKKFKGVLDSLTDTDATTSNILRFLFRVVSYMDMESGLLSMECFTDALSSMQRQPKMDSLQDLSILELYILVCMNRLEDKEQKSYNFNTIIKEYKSIQDAYKTSDKYATTVCFRAFEHLLDRELITFADTKGRNVALEYRPVKLLISSRELAQSLNLNTTCPAVLQKLLDRERYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>M0VGL2</accession>
    <name>M0VGL2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0VGL2"/>
    <dbReference type="SMR" id="M0VGL2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0482530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0482530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0482530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0582050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582050"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VGL2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF17">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE FAMILY 3 MEMBER F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="74" mass="7966" checksum="C5476CC9E14FC047" modified="2013-04-03" version="1">MGAIAAGNAVALKPSELALSTARFLEENIGEYLDANAVKVIQGGPAVGEQLMEHQWDKVLFTAIWQGACAWHAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>M0VQK2</accession>
    <name>M0VQK2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123440582</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973080.1">
        <property type="nucleotide sequence ID" value="XM_045117145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VQK2"/>
    <dbReference type="SMR" id="M0VQK2"/>
    <dbReference type="FunCoup" id="M0VQK2">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0VQK2"/>
    <dbReference type="PaxDb" id="4513-MLOC_3645.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304270"/>
    </dbReference>
    <dbReference type="GeneID" id="123440582"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0253970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0253970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0304270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440582"/>
    <dbReference type="eggNOG" id="ENOG502QSNM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0VQK2"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VQK2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF139">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="42294" checksum="90657A8A44AE1521" modified="2013-04-03" version="1">MHTSDPRVAYKDRLQRTRTSAQARTSPMAHPVLLLLLALLLLLTTPAISSSKRTQPRFPAIFYFGDSIFDTGNNNHIPTLGLANHPPYGRDFPGSRPTGRFSNGRLVPDLLNERLQLKEFSPPFLDAGLSDKDIMTGVNFASAGSGFDDRTSRVANTMPLSVQVDLFRDEYLPRLRSISGDKEATRIIASSLMFINSGTNDFTRYYRSSKRRVDIGEYQDSVLQLVHAHVKELYKVGGRQFILAGLPPFGCIPFQITLSGDPERGCVDEQNREAQVYNSKLQKLIPALQGSLRGSRIVYLNAYEAFTQIIDNSNKYGFTETTLGCCGTGLTEVGILCNRFTPICRNVSSYIFYDAVHPTERVYRIATDYIVKNVIPQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0WMH1</accession>
    <name>M0WMH1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123403033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044952946.1">
        <property type="nucleotide sequence ID" value="XM_045097011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WMH1"/>
    <dbReference type="SMR" id="M0WMH1"/>
    <dbReference type="PaxDb" id="4513-MLOC_52938.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614100"/>
    </dbReference>
    <dbReference type="GeneID" id="123403033"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0509160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0509160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0509160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0614100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403033"/>
    <dbReference type="eggNOG" id="KOG1124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WMH1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044534">
        <property type="entry name" value="TTL1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46050:SF7">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46050">
        <property type="entry name" value="TPR REPEAT-CONTAINING THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="570" mass="61209" checksum="A4767DB66AD746BE" modified="2013-04-03" version="1">MTESRRAPSGCAMFGIYSGMFRRRRSASMSSLSRINGSPQAAAADSDAAPANPAQRKAGVHDDSALVPRPKVMPLQPQINGAAAQAAQRPSGDKSRPNRTMNGGAKAAAASPAVEYTGMAAELDKMIHDHQRVKGTTQMMRATSGNMMMHRNLGNLNTGASARSSLDRSAKAAAANDRAKAGPPGSNGYAFSGMGNIVSKPADQLCRALSHRTDPEKLKEMGNEEYREGHYAEAVALYDQAIMMDPTRPAYWSNKAAALAALGRLIDAVADCKEAVRIDPSYGRAHHRLGGLYLRLGEPDKAINYLKQSPVDSVSADVSRAQSVKGRIAKCTDARKVRDWITVLQESQAAVSDGADCAPQVMALQAEALLKLQRHDEADSALRGALRFGVDESAKTFGTTAHAYVLMVRAQVDMAAGRLEEAVATAQTACELDPGSRETANVHRRAKVVASARQRGNDLFKASRFAEACAAYSEGLDKGEGGSAVLLCNRAACHAKLGRHEKAVEDCNGALAVRPGYSKARLRRADCNVKLERWEASLRDYQVLIQELPENEDVKKALAEVEVKLKSQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0Z5F5</accession>
    <name>M0Z5F5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase 61 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123442420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044974392.1">
        <property type="nucleotide sequence ID" value="XM_045118457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Z5F5"/>
    <dbReference type="SMR" id="M0Z5F5"/>
    <dbReference type="FunCoup" id="M0Z5F5">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0Z5F5"/>
    <dbReference type="PaxDb" id="4513-MLOC_7745.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221240"/>
    </dbReference>
    <dbReference type="GeneID" id="123442420"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0183990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0183990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0183990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0221240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442420"/>
    <dbReference type="eggNOG" id="KOG4698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Z5F5"/>
    <dbReference type="OMA" id="PAMVFAM"/>
    <dbReference type="OrthoDB" id="529273at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Z5F5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF28">
        <property type="entry name" value="OS01G0118700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="9">
        <location>
            <begin position="317"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="525" mass="57841" checksum="BEE8DE4509FCD879" modified="2013-04-03" version="1">MGDDHGAKLVKSLRGAAQKYVGVGFFLGFFLVLLTYFTVSEQFAIAAPNAIRRTSPGHPTLTSPAVAEKTVQQQQLPVKKEEAPLPEHEQVHEKPPPVDDDEEPHRETEEPNSQTEHPIDEASKIVTTAEESAPAKKPACDIQGPWASDVCDLAGGSGGVRIHGSAHTVLVPPTIESGGSNPNPQEWRVLPYSRKHMSGIKEITVRELATPADAPRCAVTSQVPALVFAMGGLTGNYWHDFSDVMIPLYLQASRFDGEVQLVVTNYQRWYAGKYRDIIARLSRYDVVDMDKDDQVRCFPSAVVGIRMHKEFSIDPAKEPTGHSMPEFTKFLRNVFALPRAAPMRVTAGPSDGKKKPRMMIISRRHPRKLVNVDEVVALAKRIGFEVVIGDPPFNVDVGDFAREVNAADVLMGVHGAGLTNSLFLPTGAVFIQVNPFGKMEHIGEVDFGTPAVDMGLKYMSYSCGMEESTLVDTLGRDHPAVKDPESIHRSGWNKVAEFYLGKQDVKLDLQRFEPVLLKAMAMLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287PKD8</accession>
    <name>A0A287PKD8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A287PKD8"/>
    <dbReference type="SMR" id="A0A287PKD8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0399670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0399670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0399670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0399670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0399670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0399670"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287PKD8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0399670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28702" checksum="5FF62E44DCB096F8" modified="2017-11-22" version="1">MSLPSMIIKLTLGFLWCIIHLAISLLSLCSLLIFNLECCLISSGLLWKYWNLQLAKLKYLAIVVDSREAKNTVKINQLLCWLKTLGVKYVCLYDIDGVLKKLFEPGMDGSRDNKPGDYLDVSANTKALGCCQRQMTIECISGSDGKEGIAKAANLLCSTFLNGDSYTQENVKSEENIKSEPVFTETDMANALKSIGCAGPEPDLLLVYGPARCHLGFPTWRLRYTEIMHMGPLKSMKYGALVKAFHNFSKKYQNYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287Q446</accession>
    <name>A0A287Q446_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123450050</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class III subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983384.1">
        <property type="nucleotide sequence ID" value="XM_045127449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287Q446"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0417970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0417970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0417970"/>
    </dbReference>
    <dbReference type="GeneID" id="123450050"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0348410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0348410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0348410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0417970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0417970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0417970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450050"/>
    <dbReference type="OrthoDB" id="125004at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287Q446">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044830">
        <property type="entry name" value="HD-Zip_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013978">
        <property type="entry name" value="MEKHLA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950:SF28">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HOX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08670">
        <property type="entry name" value="MEKHLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="77"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0417970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="837" mass="91522" checksum="E127469392A93B64" modified="2017-11-22" version="1">MAAAMAMRGGSSDGGGSDRAGMDSGKYVRYTPDQVEMLERVYADCPKPTSSRRQQLLRECPILANIEARQIKVWFQNRRCRDKLRKESSRLESVNRKVSAMNKLLMEENERLQKQVSQLVHENAQVRQQLQNTSMANDTSCESNLTTPQNPIRDASNPSGLLSIAEETLTEFLSKATGTAIEWVQMPGMKPGPDSVGIVAISHGCRGVAARACGLVNLEPTKVVEILKDRPSWFRDCRSLEVFTVLPGANGGTIELVYTQLYAPTTLVPARDFWTLRYTTIVEDGSLVVCERSLSGSGGGPSAASAQQFVRAEMLPSGYLVRPCDGGGSIVHIVDHLEFEAWNIPEVLRPLYESSRVVAQKMTTAALRHIRQIAQETSGEVVYALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSIMAGDGMEDVVVACNSTKKIRNISNGVNAFEAPGGTICAKASMLLQSVPPAILVRFLREHRSEWADYSMDAYLASALKTSTCSFPGLRPMRFSGSQIIMPLAHTVENEEVLEVVRLEGQPLTPDEVLLSRDIDMLQLCTGIDEKSVGSSFQLVFAPIDDFPDDAPLISSGFRVIPLDMKTDVVSSGRTLDLASSLEVGSTAAHASGASPDDCNVRSVLTIAFQFPYELHLQDSVAAMARQYVRSIVSAVQRVSIAISPSQSGLNAGQRILSGLPEAATLARWICQSYHYHLGVELLTQSDVAGEALLKMLWHYQDAILCCSFKEVPVFTFANKAGLDMLETSLVALQDLTLDKIFDEPGRKALFAEISKLVEEGHVYLPSGVCMSGMGRHVSFDQAVAWKVLGEDSSVHCLALCFVNWSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WRU5</accession>
    <name>A0A287WRU5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123412061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960950.1">
        <property type="nucleotide sequence ID" value="XM_045105015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287WRU5"/>
    <dbReference type="SMR" id="A0A287WRU5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0696220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0696220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0696220"/>
    </dbReference>
    <dbReference type="GeneID" id="123412061"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0577830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0577830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0577830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0696220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0696220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0696220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412061"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287WRU5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000132">
        <property type="entry name" value="F-box/kelch-repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000105">
        <property type="entry name" value="F-box/kelch-repeat protein At1g51550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175">
        <property type="entry name" value="BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175:SF4">
        <property type="entry name" value="BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="14"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0696220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="444" mass="48863" checksum="71DE2321B7B07EA4" modified="2017-11-22" version="1">MDAGAVGHLGYDQVLSILRLLPAEAVLSFAATCRAFRDWASSDVLWEALCRRDWGARAAAAALVDWRCGVPWRRLYAEVARLSALQARRLQVKGTSPRPRASHSLNLVAGWLLVFGGGCEGGRHLDDIWATYVGNGAGTGSSNVLSWQQLASGTPSGRFGHSCVLVGDALVLFGGINDRGLRLNDTWIGEIICEEPGRMMISWRLLQIGPLAPSPRGAHAACCIDDKLIVIHGGIGVHGSRLGDTWLLDFSDGLRSGSWRQMGDTGPLPSARSGHTLTWIGDRRMVLFGGRGSEYEVLSDVWLFDIGDHFLQWKEQKYNLSSILGELPSPRAGHSATFLFGGKILVYGGEDKERRRMDDFWILDIPALLQFESGTRKMAKRMWKRLRIDGQSPNCRSFHGACVDTSGYRVYVFGGMVDALMHPAESLGLRFDGQLYQVELVLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287XIF1</accession>
    <name>A0A287XIF1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123410611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044959489.1">
        <property type="nucleotide sequence ID" value="XM_045103554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287XIF1"/>
    <dbReference type="SMR" id="A0A287XIF1"/>
    <dbReference type="STRING" id="112509.A0A287XIF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0728740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0728740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0728740"/>
    </dbReference>
    <dbReference type="GeneID" id="123410611"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0604510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0604510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0604510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0728740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0728740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0728740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410611"/>
    <dbReference type="InParanoid" id="A0A287XIF1"/>
    <dbReference type="OMA" id="GIPHIAP"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287XIF1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF173">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="533"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0728740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="720" mass="80021" checksum="B5375E11B885B908" modified="2017-11-22" version="1">MASDFDEDQNVPYAHPAGPPALDRADNVRLRGHASDRAPLHENRQQVLLEVIDASSAGGDRRLGLDLVAVLDVSKSMRKNDRLGKMKTAMHFVINKLGHMDRLSIVKFSEEAERLCPLLSVTPPNKARLNHLVDGLQVIDPTNIRDGLEAGLSVLAGRRITGGRVASIFLLSDGDENRGHATTVDVSDVPVYTFGFGTDYDPKVLDEIARRSKGGTFNFVDDEENMTEPFSQILGGLLSIVVQDLKLIVSPQRGESIIEGVDSRLYQQTWDAGSGSITVHFGDLFAGEVRRVIVHISLPEVNQKKDVTAIIAQCSYSVRGQPLYSRGLAVPMRRTPTGSADLSRMRPVALRNELARGRHVTFLEEARTLADNNDLHGADGKLVEARNDLAPEQSNSMIHTLRAALDKLLEMIRSPGGPRAGFAAYLRSLKTSHDRERVSATGDVKGVRLFETRHTSAFRGQAKRFEKQSTMGRRPAPHEFKEEEEDANRLREEDGRMERRPVLVEARSGLWKTWWGDDDKQHRRSARSTSACAWVMCILCLLLIVGLIVLGVLLFAVYNHKMPYLAVAGAQLGALQYDGQAGTVQNLQISIAFLAINRDSKADAYFSRVNLALQLHGVDMLLLRAPPFVVPPDTSMPLQYNDVVSTGRRLDKAGMRSMDKSLNAGVVPFDLRGKVRMRGKTGIFQNTQFWTRFSCRLRFFFPGNGTVMPVDRRSCRSRWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W3Y7</accession>
    <name>A0A8I6W3Y7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6W3Y7"/>
    <dbReference type="SMR" id="A0A8I6W3Y7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0007770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0007770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0007770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0007770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0007770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0007770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF92">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0007770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="217" mass="24275" checksum="DD7B4DB11F79AA49" modified="2022-05-25" version="1">MPGGRTAHSRFKIPLSIEDGASCSFTKQSGTAKLLRMASLILWDEASMTKRQAVEALDNSMRDITGRRHQPFGGKAVVFGGDFRQVLPVVRKGSRGQIIDATPRSSYLWKGMRQLRLITNMRAHNDPWFADYLLRVGNGTEETDDEGNISLPQDICVPPTGDGVDLEKLIDHVFPALDHNMSDPNYMTSRAILSTKNDNVDKINARMIERFQGEEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W4C1</accession>
    <name>A0A8I6W4C1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W4C1"/>
    <dbReference type="SMR" id="A0A8I6W4C1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0015530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="46"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="219" mass="25467" checksum="CD7CF31E0E507F0B" modified="2022-05-25" version="1">MPTTFHEVVAHGATKLRVMYTNLSTEVPYFLQQFKERWFNHAPDHEKFFGLDLEYTADQRSVAVIQICFTSHVLIFQWAKSDKHCPGLMEFLRSGVTFASVDIRNDRLKMRHSFGIEIPAGCLIDIQTIFKLQHDRTSMARMAVALIDDSYADMKTSFPRSQHRLWEKGPLDDINIEYAAKDAYVSYELYRKIRVVHYGQRHLYPLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W6K6</accession>
    <name>A0A8I6W6K6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123441421</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973801.1">
        <property type="nucleotide sequence ID" value="XM_045117866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W6K6"/>
    <dbReference type="SMR" id="A0A8I6W6K6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062760"/>
    </dbReference>
    <dbReference type="GeneID" id="123441421"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441421"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46692">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46692:SF1">
        <property type="entry name" value="NUCLEOSIDE HYDROLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035204305" description="Inosine/uridine-preferring nucleoside hydrolase domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="3">
        <location>
            <begin position="37"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="3">
        <location>
            <begin position="509"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="884" mass="97532" checksum="98A6C5D7D6A2DECA" modified="2022-05-25" version="1">MEKWRRTVVAAVVAIMVSVVVVAAGGAPTDQAAPQRILLDTDMDTDDLLALMYILKQNRSEFELKAVTINVNAWSDAGHAVNHLYDILYMMGRDDILVGIGGDGGISDAGTIYPNVGGYLPLIDQGITTVGDCRYRKAVPLEGGGRLDVDTNWGIRRSFLPQGNRRYIPFQQPTTQQVMVDTISAGPTTVILTGSHTNFAIFLMTYPHLKGNVKHIYTMGGGVRSKNPTGCCPKNVTTACTPQQCGDIGNLFSSYSTNPYAEFNIFGDPFAAYQVFHSGIPITLVPLDATNTIPVNEEFFYAFQQHQSTFEAEYCFKSLKMARDTWPDDQFHASYFMWDSFTSGVAISSMRNDKDCQHENDFAELEYMNITVITSNEPYGIYDGSNPLFDGHATPKFGLKKGGVHSGHVQTGITDNFCLIEGSRKGRCEDGYTKEVSGLEAVPVRVATKAKSYADKNSPLDREFFKSFLEVLTLRDNSGRFNITEQFPFYREVLYKPDFVNKSRGNAFIFDIDMSAGDFVSLIYLLKAPVEVIDLKGIFVSSSGWANAATIDIVYDILHMMGRDDIPVGRGNSTALGTGTLGCKYVSVIPQGSGGLLDSDTLYGLARILPRSPRRYTAENSVEHGAPRNTDNPELRQPLAFEVWQSVKEQLDPSEKITILTNGPLTNFANIVLSDTNASSVIKSVYVVGGHIRDENDSNGNVFTVPSNRYAEFNLFLDPLAAKVVLESTMDITLIPLRSQRKASCFRTLLEALEYAENTPESSFVLHLLSLLHDLQQKHRLYHHMGIFLGELLGAVYLVEGSNMEPSLLLKPISIVAGNTTSTDGQVVVDEQSANSVKVLVDFDSDEYYSRVANHLGSMERSAVIGSFAEQRASWSKQPDNLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W907</accession>
    <name>A0A8I6W907_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Meiosis-specific protein ASY3-like coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123431518</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971272.1">
        <property type="nucleotide sequence ID" value="XM_045115337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W907"/>
    <dbReference type="SMR" id="A0A8I6W907"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097780"/>
    </dbReference>
    <dbReference type="GeneID" id="123431518"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0080670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0080670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0080670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0097780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431518"/>
    <dbReference type="OrthoDB" id="751607at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037731">
        <property type="entry name" value="ASY3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046845">
        <property type="entry name" value="ASY3-like_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36027">
        <property type="entry name" value="MEIOSIS-SPECIFIC PROTEIN ASY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36027:SF2">
        <property type="entry name" value="MEIOSIS-SPECIFIC PROTEIN ASY3-LIKE COILED-COIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20435">
        <property type="entry name" value="ASY3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiosis-specific protein ASY3-like coiled-coil" evidence="3">
        <location>
            <begin position="726"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="582"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="794"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="599"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="900" mass="98374" checksum="8F99B9479D76C483" modified="2022-05-25" version="1">MTEIVLPNMQKAASSDYWSLASNQYPSGKFPKVSIGIPIPRTGSVSGSRDAAAAAAAAPAFERNPSQGTDGRSRPPKAKGHNASLRVSQEAANHGGSATEAPEAAHVRGSLSQPDDHAREQTGTFSFGTRKEQGSQLDQLQKTAFVNSQGKCQVESADKTKPNSEVLRMKLWGILGTSQTKQAVASPNLEDIETPDQPKSLTTNVPSLGIKKVYTSRFPDIIKTPDLLNCQTATYAKSKPSSDPIESDSDTPQVVEIRPVTRNLGRKKAPAASKKQDKSQSAKKPLPTSRSAPKQKKLDSVFVFDEKCTPKTVGKSANGNSGCLRNLRSSSRKAKAELKKVHCSDTISDKITQDARGQLSSRNAPSENKGEKTTSLSSLSRTGKTAESRSRSPTREKRLNGMAKVGPQKMQLSEKLLPKTLNEGEDKLSSQKISSKSKENYSSVPHRKENSNLSKASVRSPQAHKPVGNNLKSPPSGAAAASPSPEPKMYPRGNEASPQINGKPSGAASPSPEPKMYPWDNEASPQINGKPSGAASPSPEAKMYPWDNEASPQINGKLGEKFASPLADRFRDMGDHFASPTFATKVLHDDTYSPKYPKSVNRSRSSSYASDPGSEPSDGMDKTYELPKSESPNSSEERENKKQPDLSPILPTEDETAQISVPSFGKGYKSRKWLSDVDSPDKSPPLKLDRKSRDGKRAKRRLPSPVPFATSGTEETIMSEKEPVQCPDDYLTRAFDELLLVLGRFQTKIKSETRNRSSEILSATGEIIRQHLEGVEVQMQDDVDKLVNAGKSKRKRLNSTFEEQQEQLRVLHEKFKEEVNQQLLGCKNSLEELEAYHSELKGVAEKQKASHKKLLQQAESRVGTQLNDAEIKIAKVQKRARRKMNGLKHVLKELITETAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCP0</accession>
    <name>A0A8I6WCP0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab3GAP catalytic subunit conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965621.1">
        <property type="nucleotide sequence ID" value="XM_045109686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141880.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141880.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141880"/>
    </dbReference>
    <dbReference type="GeneID" id="123425922"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0141880.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141880.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425922"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045700">
        <property type="entry name" value="Rab3GAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026147">
        <property type="entry name" value="Rab3GAP1_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422:SF10">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13890">
        <property type="entry name" value="Rab3-GTPase_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3GAP catalytic subunit conserved" evidence="2">
        <location>
            <begin position="338"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141880.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="665" mass="73325" checksum="16A1B40AEFE739AE" modified="2022-05-25" version="1">MHQLPPPPPASSTSLVSRARTAIHSAAARAERVLTDIKADLRDADGSSGHRTPSPRTSMDRQVDAEAAAPASGQTPDMKPPPDEVEVPEVIPSADKDGLKIEHGSTSSSNLAFPPASIVKQLIAAIEDGKKFSSMNDMKFNGDQYLKEKGGLSLSVVKSLVRREKEDRSSSEFFGDDETKSLMYSLFKFAEELPHDESQCGPELLHSTSLSRDIHGAPPGSFVHHLGEIIGSISSVHKMAFFWQSVVVELKKLWSDGQPVPRMSLHAAPDLNCCLLYQEIQVINCCIARKKRRKAAKESLDSLLKQECVDNSNPRYSNGDSRDSGIYARNSSGDCVLRLGADCASENLTLLETGESVYCPILQEGPIMTAELIKETEELVLRTGSVGAGCSQLLSDMQAFKAANPGCVLEDFIRWHSPPDWSEDFAASNATVGEGSSRRGRLSDRMQTKEGNLWKELWEAAKPIPAVEQTPLYDEDLAVESIFDALEVIEPAKLFQQLLSVILSVCFVAAESVLPADSNLSKLFYDCKDYIIGIYQDDMSKEKLDEICKVYETMEAIVTHPEDALQITEQPDEKSAEIKNRFKLKLNFNGKDGQSLWKRAAKDEKRTSPNDEKKSPEEKNSNFFSNLRERKAALFSKKNAKASELPSPPPPSALGPFDESEWTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WE99</accession>
    <name>A0A8I6WE99_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">starch synthase</fullName>
            <ecNumber evidence="6">2.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123425502</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Amyloplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044965135.1">
        <property type="nucleotide sequence ID" value="XM_045109200.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6WE99"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0022500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0022500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0022500"/>
    </dbReference>
    <dbReference type="GeneID" id="123425502"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0017860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0017860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0017860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0022500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0022500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0022500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425502"/>
    <dbReference type="OrthoDB" id="639559at2759"/>
    <dbReference type="UniPathway" id="UPA00152"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (ADP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010021">
        <property type="term" value="P:amylopectin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000165">
        <property type="entry name" value="Starch synthase, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005085">
        <property type="entry name" value="CBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083:SF5">
        <property type="entry name" value="STARCH SYNTHASE 3, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16760">
        <property type="entry name" value="CBM53"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01066">
        <property type="entry name" value="CBM_25"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="15">
        <location>
            <begin position="709"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="15">
        <location>
            <begin position="884"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="15">
        <location>
            <begin position="1047"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="820"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0022500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1593" mass="178795" checksum="B60DD85FFC3B5353" modified="2022-05-25" version="1">MEMALRPRSPLCPRGRQPLVVVVCPAGLAQPFWMNGRFTRSRTVRCMVASSDSPNRKSRRLVSPQVKVISSRGYTTRLIVEPSTENTEHNNGDEEILDTYNALLHTEAAEWTDTREAETAEADSSQNASSSSIIRELDAADEDILAVDLTVNALSSVTKREVDAADKARVEEDVFELDLPATTLRSVIVDVIDDNGTVQETLRSVIVDVTDDVADKARVEEDIFELDFSGNVSSSATTVELDAVDEVGPVQDTFEASLSGNVSNSATVQEVDAVDEAGNDQDIFKADLSGNIFSSSTTVEVGVVDEAGSTKDRFEMDSSGNVSTSATMWDAIDEAVADQDAVEADLSGNASSWATYRELDDMVDENRSEEETFVMDLVGEATDEEENYQQQYPVPSSFSMWDKAIAKTGVSLNPEPRLTSVQEQGKVNFSDKQDLSIADLPGQNQSIVGSCKQDKSIADVAGPPQSIFGSSKQHRPIVAFRKQNHSIVSDPKQKQSIVGFRSQDLSAVDLPKQNIPIVGTSSEGQTKQVPVVDRQDALYVNGLELKEGDHRSEKTDEDALHVKFNIDNVLQKHLADRTQAVETTIWKEVDEEHLYMTEHQIGSTEGHMVLNEDELSITEIGMGSGDKIQHALSEEELSWSEDEVQLNKDDGQYEVDETSASFTVEQDIQGPPQDVVDPQALRAMLQELADKNYSMRNKLFVFPEVVKADSVIDLYLNRDLTALANEPDVVIKGAFNGWKWKLFSERLHKSDLGGVWWSCKLHIPKEAYRLDFVFFNGRTVYENNGNNDFCIGIEGTMNEDLFEDFLVKEKQRELEKLAMEEAERRTQTDEQRRRKEARAADEAVRAQAKAEIEIKKNKLHSMLSLARTCDDNLWYIEASTDTRGDTIRLYYNRNSRPLAHSTEIWMHGGYNNWTDGLSIVESFVKCNDEDGDWWYADVILPEKALVLDWVFADGPAGNARNYDNNSRKDFHAIIPNKNVTKKGFWVQEEQNIYTRLLQERREKEEIMRRKAERSANMKAEMKAKTMRRFLLSQKHIVYTEPLEVRAGTAVDVLYNPSNTVLNGKTEVWFRCSFNLWMHPSGALPPQKMVKSGDGSLLKATVNVPPDAYMMDFVFSESDENGIYDNRNGMDYHIPVSDSIETENYMRIIHIAVEMAPVAKVGGLGDVVTSLSRAVQDLGHTVEVILPKYDCLNQSSVKDLHLYQSFSWSGTEIKVWVGQVENLTVYFLEPQNGMFGVGCVYGRNDDRRFGFFCHSALEFILQNGLSPHIIHCHDWSSAPVAWLYKEHYSQSRMASTRVVFTIHNLEFGAHYIGKAMTYCDKATTVSPTYSREVAGHGAIAPHREKFYGILNGIDPDIWDPYTDNFIPVPYTYENVVEGKSAAKRALQQKFGLQQTDVPVVGIITRLTAQKGIHLIKHAIHQTLESNGQVVLLGSAPDHRIQGDFCRLADALHGVYHGRVKLVLTYDEPLSHLIYAGSDFILVPSIFEPCGLTQLVAMRYGSIPIVRKTGGLHDTVFDVDNDKERARSLGLEPNGFSFDGADSNGVDYALNRAIAAWFDARDWFHSLCKRVMEQDWSWNRPALDYIELYHAARKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WEA5</accession>
    <name>A0A8I6WEA5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WEA5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076990"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076990.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17336" checksum="DD6DD4574826A378" modified="2022-05-25" version="1">MKREQEATSLREKIDAMVQSNELMLLKSLEIKKELAEKKAKEKQEKWQMLKEEERKASVSENKSMSLLLTEENKIMSMNRNDMEDLTKTWHDMARREILKRRMVASAGPCSSSGDVFSAPYGANVDDFDARVGTSDGGGFGGADELQYGLHGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WEQ3</accession>
    <name>A0A8I6WEQ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WEQ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN / LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877:SF3">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN _ LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="704" mass="70778" checksum="FA40F6E9189DF995" modified="2022-05-25" version="1">MASQQQSRKDAASKREEGQGGVGLEEIGKFRAEAQQHSADAIRAAQERYNQNLQHGGGARGAVTVTQAPGATVVSYQEHKAIPEGAHQGRAHGHGANAPAGGTTASSRGTELQHGAKQEEGRGHGTGHKEHKGSAAVTRAADDKESAARGTNAPAGGTVASSRGVEKQHHTKQEEGRGHGAGHKEQKGSAAVTHATEDRGKDGDSARSAKDAAMHALGLTGDQTVGKGAGIKDAGAHGAHVRGHDAGHREQKRSDSATRTLGSTGDYAAAKGAETKDAGAHGAQVTAEGTQEATATAAEYAKQVAAKAKEVTVSTGGTAAEYAKAAAEKAREAALAAGKTTAEYTQQAAVKGKDVTVSTGGTAAEYAKTAAEKAKDAALAAGKTTAEYTQQAAVKTKDVTLSTGAQAAQKAKEVTAVTAQKVAEYTKEMAEQGKATAAEVEEKAKEAAARAADKAEEPSLDTSSQAKGSAARAADKTRDTAAQTMGRARDATGQTGDMTGSMAGQVKDTTGAMAQKASDTAAYIKDSVKGAAGGTADRTRDTTGQTMGRAKDATGDVGDRTGSMAAQVKDATGAMAQKASDTAAYIKDSVTGAAGGAVDKTRDAASQVAQKTGEVKNRAVDAGKNATASGTTGTAKAKGGGTDGARIVEDALEVVGATVEAVGATVYGIAQHTKGIVAGEEELIPVEREAAKTAGAGERREKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGS5</accession>
    <name>A0A8I6WGS5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123404871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044954743.1">
        <property type="nucleotide sequence ID" value="XM_045098808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGS5"/>
    <dbReference type="SMR" id="A0A8I6WGS5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0007300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0007300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0007300"/>
    </dbReference>
    <dbReference type="GeneID" id="123404871"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0005410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0005410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0005410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0007300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0007300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0007300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404871"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF20">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0007300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="13813" checksum="54F7D2298E9EED9B" modified="2022-05-25" version="1">MASSLKALLVEDTAVQRMVLSALLRKQFHCEITMAENGKEAVDMFLEGNTFDIVFCDKDMPIMTGPEAVGKIRAMGATDVKIVGVSTDFEAMEAFMSAGADVFVPKPIRLDVVEPMIQEVINKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WI55</accession>
    <name>A0A8I6WI55_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF569 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WI55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0061810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0061810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0061810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007679">
        <property type="entry name" value="DUF569"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054726">
        <property type="entry name" value="Ubiq_DUF569-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205">
        <property type="entry name" value="ACTIN CROSS-LINKING PROTEIN (DUF569)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205:SF7">
        <property type="entry name" value="DUF569 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04601">
        <property type="entry name" value="DUF569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22932">
        <property type="entry name" value="Ubiq_DUF_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF569" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="DUF569" evidence="2">
        <location>
            <begin position="214"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="32878" checksum="003A25765E8FBFDA" modified="2022-05-25" version="1">MELFKDAWCVRLRSHLGTYLCALDGEAVSHGYRRNSRGTVWAVELAGDDYVRLQGQRGLYLGATEIPADILGRGRGTRACCRVVQGSPSCPNDNAFLWSPLREGEHLALSGPYGRLLRARFGHTAQDNAVTVDLDADPEGSSWVVEVVPAAEAALLLLPSPRACRAQSCDARLEAITSESDTASSVLARLRSAKDMSSSLGAVEEEPLSMPAPRLIFYNTARDDGGVDEFDEGTWKYFSFKEQSLAALHRRLAEETRRQDFVVCRRTGPGLFPVVLDLPPGNSQMEFVLVPASSPAVPDGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIF6</accession>
    <name>A0A8I6WIF6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIF6"/>
    <dbReference type="SMR" id="A0A8I6WIF6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0064810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064810.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0079530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF98">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="291" mass="30278" checksum="ED73EE9DE6791D26" modified="2022-05-25" version="1">MAMSTVGKQWTRVRTLGRGASGAEVFLAADDASGELFAVKSAAGASSAAALRREQRVMAGLRFPRVVSCIGGRAARDGSYQLFLEFAPGGPCGDQRGARRARGPRGTNGVRGRRGDRAGVPPRGRDCARDVKSPNIVIGDDGRAKLADFGCLRKAGAAADVPIIGSTPAFMAPEVARGEDQGPAADVWALGCTVVEMATGHAPWSGMDGDALAALHRIGYTQAVPEVPESLSADAKDFLARCLVRQASDRCTAAQLLEHPFLASAGVGVYAKPQAAESKWVSPKSTLDATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM13</accession>
    <name>A0A8I6WM13_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WM13"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18557" checksum="BC77A26176713590" modified="2022-05-25" version="1">MRKRQWQGDIKCSFCSGLESAQHLFFGCSVAKIVWRTLGAVIGTSYVPKTIWQVYSWLYAFLPGFYEIYTVGLAAVCWSIWLARNRATFEKKFINTPFEIAFTTSAFLDYWTGMQKPEMTDNVKKGAQLLKRSAAQMLHLCEPSRAEATERDEGEGIWDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WN38</accession>
    <name>A0A8I6WN38_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123442543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974547.1">
        <property type="nucleotide sequence ID" value="XM_045118612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WN38"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0225300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0225300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0225300"/>
    </dbReference>
    <dbReference type="GeneID" id="123442543"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0186980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0186980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0186980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0225300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0225300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0225300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442543"/>
    <dbReference type="OMA" id="EEYWYLL"/>
    <dbReference type="OrthoDB" id="593438at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF16">
        <property type="entry name" value="RX N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0225300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="56054" checksum="F7FE919E62626EEB" modified="2022-05-25" version="1">MELPISAVTSELVSRFVSFLLNKYQSHRHTRLEQQNLVEKLQHLLIRARTVVEEADRRCIANSGMLMQLKMLSEAMYRGHHVLDVFNFQQLLDSSIIDEVSDSSYLSFPFKRPRRIASKAKGRTMQLELHGALKSLGIVVANMAEFVVLLGGCERMSRRPYDAYLYTDHFMFGRHVEKQKLLGFLLQENPVSDEPVVLPIIGAVAVGKKTLVAHVCNNERVRSHFTSILHLNGDSLLRIIEHDRTMLGSRFLVVVDFVSHVDDKDWTKFYSFLRRMGKGSKVIIISKLQTLARFGTVKPIYLNPLSYEEYWYLLKTLTFGSADPSEHPQLVRVAEEFAKIFHPGGSLMAANAFADVLRRNFDVEFWLCLLGKTRGVIQKNLSEHGVDPYQRFEQGHTVDITDFTLHPSTAIRIANCTNGSNRLTNGAPMKMETVTLGELLVNPNNRSKGEFILLSWESRIPPYTRFEHIVASSVQDLTEGVNLPGSKKRQVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNJ9</accession>
    <name>A0A8I6WNJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123426379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044966134.1">
        <property type="nucleotide sequence ID" value="XM_045110199.1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0157640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0157640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0157640"/>
    </dbReference>
    <dbReference type="GeneID" id="123426379"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0157640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0157640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0157640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426379"/>
    <dbReference type="OrthoDB" id="1572276at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF80">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="2">
        <location>
            <begin position="90"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1786"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1877"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1916"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1891"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1916"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1932"/>
            <end position="1944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0157640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="2131" mass="234492" checksum="A1AD27442E139BF2" modified="2022-05-25" version="1">MSSDELLLVQELPMDMHSDGGAPQRRLAHVLLPLDRAPGPPPFRPPPPPSAATAAEFRVAFRGWLGAPRHWELWVAKLRPIHERLWRELGIFDAVLASTYRIKRDAPAVLHLASFWSPSTSTFAFPWGEATITLEDVAVLGGFPADGSPVPAPLPPQLRPDEAALNGVRLDFNRSACKKAHHAAWMKHFLTDTDMDAVIEHAAFLALWLTRFVLPGHPESTMRQSLFPLAVRMARGDRVALGPAVLASLYRDLREMKTYLVAAGATGGNAELLSPLSVHAPLYILQLWMWERFPVLWDGKPNLIKDGEPRAASWHDVSKKMNPMMIREVLNSGKNFLWRPYTSSEQACKDHSGWVRGSDINEDEELISLAHCLHACELVGMDCIEQYLPHRVAMQFGLDQDVPGDVRRANEDWGVAWETYDLEGKSVAIFTPCSEPGVTARYAEWWRQPLPPDLDVGAGSISVEGKLSKRKVKKTRAAMEAEVEKERKMKKARISPNNDKKRKLEELYDAKLSDWLAAGRNGSSGTSGGSCKRGSSPKSDAGSDKSLSASAGTRDDIVLVPRKQLPTPTVNLRKDTDMNLGRGDDGNFMTKSLVGTKYKGVYLKGSMAGDVDHLVNEPYFDETVVSTEISTFNEDAKDEWSDQILKNTSELVSGEPPAVPNMPEEIKPSISIEREECSDLLTDVDFSEDGTEEGITVDKPTNFSTAPEGGDAVMMEEKIVNLLEDKHLDAAARPEEGTIITQELEKKAKLVVDESCGMSNRPEEVVALVTKDIEEKGNAAVAIEGSAVSEGVGRVGEGTSHSVGVLSGSKQGAYAGVTNNPQETAALPEEILPVQQANDVGECVGMSCIMEDTCAAAGVATNDETALDFVMDEEREISCKEEIGGESNQMAMKDSEQKPQEAPPVDIVECGEDITLMKKDNKGEHGNVPQTVEAVTTGINMTAVLLDVPEEGSADVDKALNLTQKDAEDVHKKVAEVEDAEQVQINTMANDGAHEGHNEVAEVEDVEQVQINTMSNGGASEGHNEVAELEDAEQVQINTMANGGANEGHNEIAEVEDVDIAEAEMRAEFDTEKPGEATEAGHADMDDGKRLTGRETVEDPGDVLEGAHAEAEKARELIENNYDDKLAEFPGIAHAEMEEVNNLMKEDADEKFEEVFHIQSTKMKGTYKPMEEDTDANLREVFDVEHAETKHTKVLVKGVADKKFEENSELRSIGIDGPHGPMEESNDGKVKAVPKVNVEVEQANRTVRGNDRPDSILQIGFPARDEANCLAKKDIEEDNAEGKCTSINNEAPIEKDAKQAKKDIEEDNVEVPQGKHMNINDEAPIEKDTKQKPNAGGKDLPEKEVDGSKEIYQLKREEGEECKVLRAKDTEENTKDALGVKQAEERQGEALTAEYMYNYIEENTLVEQVDGQSKTLTRIGVEENPKDITQEQKKEFDNDTVESLKNSINSLLCSPAIIQSKEGQKEVPIAGMTEKHCLQDVGLINERESLSDGAAMEIQGVDGHKTLDMHEEVSIKQIHECGIIFENKETQILEDNHMVGNESNDLVLMEVDETWSAVEIHNQEALDLDKQQTMEERQDIGPTIENKKMAMLGNVITLGCCEFQADSPETEINEVLSTRGIHNQEHLDIKEEQVSEKELGCIITEGNARSLVVVDTLGGGEADTTVAIVDVSKANCVEGIQNMEACGKDELQARQDKQHNGVGDGRILEGMSANDSGDVGTRNQWDLCMEKEPAVLDKQDEGTTDESTGRMLVDTAAPECGEAKTDVTMKMNNDVLCTQAVNMAEDKTSSQNQQKCVPLGDHNKSDPQEPAVLEDERTTDENTGRMLVNTDAPECGEPKSDGTMKMNNDALCTQAVNMAEDKVSSQNQQKCVSLGNHNKSDVEGSGWNQTARKESKGTLQSECEHEIEVKQEILENKTELPIGRENDEASEQEQTYAENNSTAPSGMDHRGENNNKWAEESIKNYGKHVSDPVNTGCQTSKFGRPSIEEVRRIHSGSISVYLKDIKVSQEKNRSDPSKSTYINNAGYCSGHGALEPVSVSKDIKVPLHDTGRICGRDRALELVTGPPEETPRWRQEQYALNILEDVQNARIAEKTRMEMEIRILKAQIASMQRQVMNMDHVGEVISRSKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQ28</accession>
    <name>A0A8I6WQ28_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ28"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0125760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0125760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0125760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0125760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0125760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0125760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF307">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="390"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="575"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0125760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="607" mass="70088" checksum="99021389DF22F1A5" modified="2022-05-25" version="1">MENVPFNKRSLNYLCKRMNWEIAEDDIRKTVELLSELKKKDPMFADSVLVDSDSKIQALMWTNGRSRYQYSTFGDAITFDTTYRTNQYDMPFGLFVGVNHHFQSIILGGVLMRNEKEETFDRVFKEFVSLMGGKATLTILTCTPLFMIAKSHSRLLSTLFLYRLTLILETDQCRSMELPIANVLPGTKHRWCKWHVLRRAKETLGPAYTMNKELRDELHKILEYMPTVKEFEAAWQTLVQKYNLQEHPMMNQLYELRKKWAKPYFAGVFCARMTSTQRSESTNHMLKNFVPPGASMHMFIKHYQKLQFDRDAEENFAEKKSRLVSPVLKSGLPLERHAGKVYTPALFKLFQEACFKSASYYVENILAVNDTYCVTHLYADQREAWSKTSYNVKVQQPDNYLQCECGMYEHMGLLCCHAIRVMAQLRFTKIPERHIMRRWTKEACEDLPEYLKIYKGRSPILGSTTFRHTALYTTALDIVRMGDSNPEAFEFAMSKLSDAMVGLKEKANGSDDKGLEDILDKEKIDMQQNINGKTVLKNSMIVESRKRDRGRPTNARCRPCRPGYEVSQPRTKFCKKCRGKGHNSAKSPANGGQTRSRGRNPGAVSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR71</accession>
    <name>A0A8I6WR71_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429908</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969815.1">
        <property type="nucleotide sequence ID" value="XM_045113880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR71"/>
    <dbReference type="SMR" id="A0A8I6WR71"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139080"/>
    </dbReference>
    <dbReference type="GeneID" id="123429908"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0114530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0114530.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0114530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0139080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0139080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0139080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429908"/>
    <dbReference type="OMA" id="STFGNYQ"/>
    <dbReference type="OrthoDB" id="660550at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000029">
        <property type="entry name" value="Aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000051">
        <property type="entry name" value="Putative aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF45">
        <property type="entry name" value="OS07G0533800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035204306" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="81"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="435" mass="45396" checksum="D57C7DC53949FA21" modified="2022-05-25" version="1">MASLAVLLMFLSAAMATNAAAVRVGLTRIHSNPDVSATEFVRDALRRDMHRHARFTRELASSGDRTVAAPTRKDLPNGGEYIMTLAIGTPPLSYPAIADTGSDLIWTQCAPCGSQCFKQAGQPYNPSSSTTFGVLPCNSSVSMCAALAGPSPPPGCSCMYNQTYGTGWTAGIQSVETFTFGSTPADQTRVPGIAFGCSNASSDDWNGSAGLVGLGRGSMSLVSQLGAGMFSYCLTPFQDANSTSTLLLGPSAALNGTGVLTTPFVASPSKAPMSTYYYLNLTGISIGTTALSIPPNAFALRTDGTGGLIIDSGTTITSLVDAAYQQVRAAIESLVTLPVADGSDSTGLDLCFALTSETSTPPSMPSMTFHFDGADMVLPVDNYMILGSGVWCLAMRNQTVGAMSTFGNYQQQNVHLLYDIHEETLSFAPAKCSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUT7</accession>
    <name>A0A8I6WUT7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UMP kinase</fullName>
            <ecNumber evidence="3">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Uridine monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123426630</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044966423.1">
        <property type="nucleotide sequence ID" value="XM_045110488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUT7"/>
    <dbReference type="SMR" id="A0A8I6WUT7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0165360.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0165360.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0165360"/>
    </dbReference>
    <dbReference type="GeneID" id="123426630"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0136260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0136260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0136260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0165360.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0165360.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0165360"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426630"/>
    <dbReference type="OrthoDB" id="409889at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000238">
        <property type="entry name" value="Aspartate/glutamate/uridylate kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000028">
        <property type="entry name" value="Uridylate kinase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF1">
        <property type="entry name" value="UMP KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="8">
        <location>
            <begin position="111"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="7">
        <location>
            <begin position="260"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="203"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0165360.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="499" mass="53550" checksum="3F4C4A02D42A1070" modified="2022-05-25" version="1">MASCNDDFGLLGDAHPAPQAPAQPAPPQQAQAFCFVEAPAAGSAAGPFAPAREEGNHSSDRGKASHHTKRRRDRPEEFSDGGEYCSYISGGGSGGRRGRGGGVSSDYRKDREEWTDGAISSLLDAYTDRFELLNRGNLRGRDWEDVAAAVTDGHGKGSGGKSVEQCKNKIDNLKKRYKVECQRIAGSASASLWPWYKQMEQIMGNSPSPGTLKPPPATNDEKPRQQQQNSNKRCPSSGSVHTTTMVPFSRSTPLSNPKWKRVLLKIGGTALAGEAPHNVDPKVIMLIAREVQVACRHGVEVAILMGGRNVFCTDSWVAATGTDRASTHLIGMMAAVMNAVLLQASLEKIGVETRVQTALMMQEVAEPYIRRRAIRHLEKGRVVIFGGTGAGTGNPLLTTDTAAALRASEINADVVLKGVIGDDEYGCPPRSNGSAPFEHISFRELAARGTSKMDMTSITCCEENNIPVVIFNMLEPGNMSRAICGDQVGTLVDQSGRMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYU4</accession>
    <name>A0A8I6WYU4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Oryzain alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123428136</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968237.1">
        <property type="nucleotide sequence ID" value="XM_045112302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYU4"/>
    <dbReference type="SMR" id="A0A8I6WYU4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204520"/>
    </dbReference>
    <dbReference type="GeneID" id="123428136"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0170070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0170070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0170070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0204520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428136"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.160:FF:000002">
        <property type="entry name" value="Cysteine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000068">
        <property type="entry name" value="Cysteine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="161"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="9">
        <location>
            <begin position="399"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="53139" checksum="A6F0249CB88109AE" modified="2022-05-25" version="1">MEKKSTAATQSIPSATPLFLLGDAPLVSGMRTSMALLAASAALLLMVSLAAAADMSIVSYGERSEEEVRRMYAEWMAEHGSTYNAIGEEERRFEAFRDNLRYIDQHNAAADAGVHSFRLGLNRFADLTNEEYRSTYLGARTKPDRERKLSARYQAADNDELPESVDWRKKGAVGAVKDQGGCGSCWAFSAIAAVEGINQIVTGDMIPLSEQELVDCDTSYNQGCNGGLMDYAFEFIINNGGIDSEEDYPYKERDNRCDANKKNAKVVTIDGYEDVPVNSEKSLQKAVANQPISVAIEAGGRAFQLYKSGIFTGTCGTALDHGVAAVGYGTENGKDYWLVRNSWGSVWGEDGYIRMERNIKASSGKCGIAVEPSYPTKTGENPPNPGPTPPSPAPPSSVCDSYNECPASTTCCCIYEYGKECFAWGCCPLEGATCCDDHYSCCPHNYPICNTKQGTCLAAKDSPLSVKAQRRTLAKPIGAFSGIAIDGKKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0Y4</accession>
    <name>A0A8I6X0Y4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
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                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6Z4"/>
    <dbReference type="SMR" id="A0A8I6X6Z4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0193580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0193580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0193580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0160510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0160510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0160510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0193580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0193580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0193580"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd23127">
        <property type="entry name" value="RING-HC_BAH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033326">
        <property type="entry name" value="BAH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46764">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BAH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46764:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BAH1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPX" evidence="11">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="162"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0193580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="261" mass="29774" checksum="1D8D34EDAF4C40BC" modified="2022-05-25" version="1">MFFTELTKEASYIAGYFSTRVQHLLNLHVSSGLQRYIWRVRQCFIDDQQIMVQEGRLLINYVTMNAIAIRKILKKYDKVHGSVSGRDFRSKMQTEHTELLQSPWLIELGAFHLNCDSSEIDEPAGFFKNEFFKNFSCDLTATQPVMTMSISETMKYDYSLTCPICLDTIFNPYALSCGHLSCKGCSCGAASVYIFQGVRSAPPKAKCLVCREVGVFAHAVHMNELHLLIKTRTSRPKKVEVRSLEANCVKLYLSKNAYYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAC2</accession>
    <name>A0A8I6XAC2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123439251</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044971923.1">
        <property type="nucleotide sequence ID" value="XM_045115988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAC2"/>
    <dbReference type="SMR" id="A0A8I6XAC2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0225280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0225280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0225280"/>
    </dbReference>
    <dbReference type="GeneID" id="123439251"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0186960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0186960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0186960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0225280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0225280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0225280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439251"/>
    <dbReference type="OrthoDB" id="688481at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000129">
        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF902">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035182213" evidence="15">
        <location>
            <begin position="22"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="17">
        <location>
            <begin position="6"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="17">
        <location>
            <begin position="124"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="325"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0225280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="642" mass="69992" checksum="7F564553B8833759" modified="2022-05-25" version="1">MATTGVVILLLLLAPTPFLAAADFCDNVKAAGAALSRNASSSPVQFATATFGQAPDVVYALALCLGDVLDGSACGSCIANWFATVNQTQCDKVGFSYTDCIVVYGATADILAAPSNATGGSGDNTPPFQDWNIRNITTGQIPRIVNLTSELMTQTVALAATTTPKLYATGVMDMTRVGPYPNVFSQAQCTPGLSADDCSACLRRLLGMVNSTMALRMGARMGVTRCYFRYEASPFYGARPMLSLPSSSPPGPAPAPTKRRSMLWVIPVAVVPLTAAAFLFFICYRRRLKRQRKGSRRARSLEWQGKNSDFSLFEFEHLLEATSNFSEESKLGQGGFGAVYKGQLPDGLEIAVKRLASHSGQGFMEFKNEVQLIAKLQHTNLVRLLGCCSQEEEKILVYEYLPNKSLDFFIFDENKRALLDWTEIVAIIEGVANGLLYLHKHSRLLVIHRDLKPSNILLDSEMIPKISDFGLAKIFSLNDIEGDITRRVVGTYGYMAPEYASKGNFSIKSDVFSFGVVILEILSGKRNSGTQQCGGFINLLGYAWQLWEEGKCIDLVDASLVSDSHSAKIMRCMNIALLCVQENAVDRPTMGDIVSMLSNETMILAEPKQPAYINVRVGNEETSTAPESYSINDVSISITSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XAK8</accession>
    <name>A0A8I6XAK8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP2/ERF and B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XAK8"/>
    <dbReference type="SMR" id="A0A8I6XAK8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0227780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000008">
        <property type="entry name" value="AP2 domain-containing protein RAP2.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044800">
        <property type="entry name" value="LEC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140:SF56">
        <property type="entry name" value="AP2_ERF AND B3 DOMAIN-CONTAINING PROTEIN OS01G0141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="62"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="175"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="38815" checksum="C41601031DFA45AA" modified="2022-05-25" version="1">MGVEILSSTGEHSSQYSSGAASTATTESGVGGRPPTAPSLPVSIADESATSRSASAQSTSSRFKGVVPQPNGRWGAQIYERHARVWLGTFPDEDSAARAYDVAALRYRGREAATNFPCAAAEAELAFLAAHSKAEIVDMLRKHTYTDELRQGLRRGRGMGARAQPTPSWAREPLFEKAVTPSDVGKLNRLVVPKQHAEKHFPLKRTPETTTTTGKGVLLNFEDGEGKVWRFRYSYWNSSQSYVLTKGWSRFVREKGLGAGDSIVFSCSAYGQEKQFFIDCKKNKTMTSCPADDRGAATASPPVSEPTKGEQVRVVRLFGVDIAGEKRGRAAPVEQELFKRQCVAHSQHSPALGAFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XC14</accession>
    <name>A0A8I6XC14_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XC14"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0240610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0240610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0240610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0240610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0240610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF87">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="2">
        <location>
            <begin position="59"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="677" mass="78087" checksum="0C07DBAE31E4073E" modified="2022-05-25" version="1">MVWLLNDVYDVEHRAYFMSEKKMELTPLKIRSHGASSVMHYDERYTPYIEMTGLLPFVQLVSRSTPNLNAAAVTALIDRWRSETQSFHLRTGEMTVTLQDVSMITALPIEGKPLCMSTDSEGWRHQMEALIGMSPQEPEVEDGGKKDRVPASATFTWIAANFSHCPEDADDEVIQRYARVYMWYVISRTIFADGTGKNAPWMWLKALTVLDNKFSWGSAALAYLYRQLDDACRRTTKDGGVGGCMLLLSVWSWERLPVGRPKSSKWNTWNDHGNPVWQPTWAYKWDLVSEVASEVNLLYKQYTNEMDSLTPEQVEWEPYGVGTNFGDAHTFELNPLCVQERHLWLMRCPLICNWAVEWHLPHRVLRQFGYFQPHPPEWVDTDTQLHRLDRRRQRKIKDWQKHHKSYVLMFEQSVQATSIIQRTQYHQHCALAFSNYLRWFQESARVEICPPAYEEDILEEPTQYDELAQGGYSKLIREGYQTSFAPVLNFVRKEVKKQADESEDILDNTPGGKKGESALRLFIKEQGKKLRRLSNILGCRDPEYVSPSRSGSSERNTLDDSASSGARMDDGDITSAANTQRNTQEDDVDTPQAADDMTLKAFQRSTYMLKPRKEFKRYSPDDYAKGKNPVAGGSRLSRMRSMDDEVEDDDDDESDDPEQFVVARRKKLVSKRGRGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XD34</accession>
    <name>A0A8I6XD34_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123426215</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="14">subsp. vulgare</strain>
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</entry>
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                <person name="Muehlbauer G.J."/>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CPP1-like"/>
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        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="283" mass="31562" checksum="5A3195FDC894E179" modified="2022-05-25" version="1">MLAHGLATNPLRGTRSPLTPRASSAPLGLVSSLGFNRGCKEKVKLFINVDRYTKYNSPSNTQRCSRISPLASASASFGDMADSSTPIFPRIHVKDPYQRLGISKEASEEEIRAARNFLISKYAGHKPSVDAIESAHDKIIMQSFFDRKKPKVDLKKKFRELSQSRPVKAVQGRFYTPSSKFIWKTAITFVLLGVLTLVFPTEEGPTLQVLISCAANIYFLYQRLKSGWKSFFYGFGSFFASWFLATFLMVSVIPPILPGPRNLEVSTACVAYAFLFVSSTFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHC5</accession>
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            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
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        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6XHC5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0422530.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
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        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0422530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25304" checksum="2998AE9ECA1A940F" modified="2022-05-25" version="1">MPTSFHEVVAHGTTKLRVVYTNLSREVPFFLQQLKERWFDHAADHEKFFGLDLEYTTDQRGVAVIQICFASHVLIFQWARSDKHCPELMEFLRSSITFASVDIRNDKLKMRHSFGIEIPAGCLVDLQTIFRLRHDRTSMAHMAVALIDESYGDMKTSFLKYQHKLWKKGPLDDINIEYAAKDAYVSYELYRKIRVVNYGQRHLEERGHSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHZ8</accession>
    <name>A0A8I6XHZ8_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">TTF-type domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A8I6XHZ8"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308310"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="entry name" value="Znf_TTF"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="264" mass="30626" checksum="05DD343411B011BA" modified="2022-05-25" version="1">MFLISNMSFRKHLSDSNKRKIKKQKDEEVKSLKGSLNKYFKPGSSSRGPLELAIVCVEEQPTENTNATDDDMDVGNSNSSEHVNVTRSSNCESPSVDKQQPFTVDIFDPRNWDNLDNKARDILVEKGPVRNDDIVFPPDSNSRHFSLSYYSRKLRNGEVHARKWLVYSEHTNKVYCFCSKLFKSHSNKSVFAGNGLSDWKHLSDRLKEHENCVEHIKTMNTWNELKVRLAKNKTIDKDLQQQINKEKERLRQVLVRLVAILKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIT9</accession>
    <name>A0A8I6XIT9_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123428011</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Prolyl cis/trans isomerase with specificity for phospho-Ser-Pro bonds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PpiC/parvulin rotamase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044968095.1">
        <property type="nucleotide sequence ID" value="XM_045112160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIT9"/>
    <dbReference type="SMR" id="A0A8I6XIT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0200980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0200980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0200980"/>
    </dbReference>
    <dbReference type="GeneID" id="123428011"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0167000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0167000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0167000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0200980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0200980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0200980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428011"/>
    <dbReference type="OrthoDB" id="2530521at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000010">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase Pin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023058">
        <property type="entry name" value="PPIase_PpiC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF38">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01096">
        <property type="entry name" value="PPIC_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="9">
        <location>
            <begin position="122"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0200980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="25095" checksum="40813540CC8E7B50" modified="2022-05-25" version="1">MLCYVLLYNKRMGTATLTTDQTDQSFAETETEIQTPDPPKRLRSSAADAKIGTDAPAPAPAPAAGAGDAARKRPGGEDAAAAADGKRRRAEHPSSGSRDRHGHQHKAGHKAGRPGDGGKGKRETMRASHILIKHEGSRRKASWRDPDGVAISATTRDDAADLARALRDQIAAGELQFDAAARDNSDCNSAKRGGDLGPFEKGKMQKPFEKAVIALKVGDMSDVVDTESGVHIILRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLI7</accession>
    <name>A0A8I6XLI7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-ascorbate oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123441587</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLI7"/>
    <dbReference type="SMR" id="A0A8I6XLI7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0251100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0251100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0251100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0208440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0208440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0208440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0251100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0251100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0251100"/>
    </dbReference>
    <dbReference type="OMA" id="VINDMFP"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF296">
        <property type="entry name" value="MULTI-COPPER OXIDASE TYPE I FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035242885" description="L-ascorbate oxidase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="33"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="162"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="382"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0251100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="61616" checksum="73B07C3381AFE84F" modified="2022-05-25" version="1">MDRGRLIAVVLAGILHSAVVAAEDRHVFLNWEVSYSVQSPLGVSKRVITINGRLPGPLLNLTTNDVAHVNVVNTLDEPFLLTWNGLQMRRNSWNDGVAGTNCAIQPGENWTYVFQAKDEIGSFFYRPSLGLHAAAGGHGPIRVNNRPVIDVPFPQPDGEFDVLIGDWYNMDAKEMKERLDGGRGLPPPDGILINGLGPYEAEVTFKAGRTYRLRVSNVGTRTSLNFRLQGHKLLLVEAEGTYTLQKHYASLDVHPGQSVSVLVAADQAPKPYYMVVSSLFVLPELFGVATVLYAGSGEQPPSGDAPLDDRSSHNDYNRSMEQAMSIRMNLTSGAARPNPQGSFHYGEINVTRTVLLRNDVSDIGGRRRCTVNGVAFANAGTPLKLADYFRIAGVLKVVSGMPETRNPSLGTTVIDASYRDYVQIVFENRLPSMQTWHLDGYSFFVVGMAWGKWSPNARSTYNLVDAMYRSTVQVYPTSWTAVLVYLDNEGMWNLRSQDVEKRYLGQELYLRVSQGDSEVPDPRDELPMPSNALLCGKATRLKSWYNYKLGLGLGPGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLZ3</accession>
    <name>A0A8I6XLZ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLZ3"/>
    <dbReference type="SMR" id="A0A8I6XLZ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0468170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035164682" description="Reverse transcriptase domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="419"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="667" mass="77426" checksum="3AAA8E5DAC9C90FE" modified="2022-05-25" version="1">MILVKLVVGDLVLNVISAYAPQVGHNESTKREFWEGLEDLVRRVPIGEKLFIGGDLNGHVGTSNTCFERVHGGFGYGIRNQEGEDVLSFALAYDMVVANTLFRKRESHRVTFSSGLHSSQIDFVLSRREDKRACIDCKAIPGESVVPQHKIVVADFRFRIRVQRDKRAKVARTKWWKLKGEASQAFKERVIKEGPWEEGGDANLMWTSMATFLRKVAIEEFGVTKGSRREAKDTWWWNDEVQKVIREKKDCFICLYLDRSAANMEKYKVAKKAAKRAVSEARDRAYEDLYQRLNTKEGERDIYKMAKIRERKTRDVGEVKCIKDGDDQLLVKDEAIKRRWWEYFDNLYNGEVDSSTIELDDSFDDTIMCFVRRIQECEVKEALKRMKVGKAMGPDGIPIEVWRGLGDIAIVWLTKLFNLIFRSNKMPEEWRRSILVPIFKNKGDVQSCTNYRGIKLMSHTMKLWERVIEHRLIRLTSVTKNQFGFMPGRSTMEAIFLVRQLMERYREQNKDLHMVFIDLEKAYDKIPQNVMWWALEKHKVPIKYITLINDMYDNVVTSVRTSDGDTDDFPIRIGLHQGSALSPYLFDLVMDEVTRDIQGDIPWCMLFADDVVLVDESRTGVNRKLELWRRTVESKGFRLSRTKTEYMRCSFSATRHEDGKATGSAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XP15</accession>
    <name>A0A8I6XP15_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XP15"/>
    <dbReference type="SMR" id="A0A8I6XP15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0362580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="52"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="460" mass="53904" checksum="F2AB91043E55AFD9" modified="2022-05-25" version="1">MCFVRRIQECEVKEALKRMKVGKAMGPNGIPIEVWRGLGDIAIVWITKLCNLIFRSNKMPEEWRRSILVPVFKNKGDVQSCTNYRGIKLMSHTMKLWERVIEHRLRRLTSVTKNQFGFMPGRSIMEAIFLVRQLMERYREQKKDLHMVFIDLEKTYDKIPRNVMWWALEKHKVPINYITLIKDMYDNVVTSVRTSDGDTDNFPIRIGLRQGSSLSPYLFDLVMDEVTRDIQGDIPWCMLFADDVVLVDDSRTGVNRKLELCRRNLESQGFRLSRTKAEYMRCGFSATRHEDGEVSLGGQVVPERDTFQYLGSMLQKDGDIDEDVGRRIKAGWMKWRQASGVLCDKRVPQKLKGRFYSTAIRPAMLYGAECWPTKRRHIQQLGVAEMRMLRWVCGHTRKDRVRNDDIRERLGVAPIEEKLVQHLLRWFGHIQRRPPKAPLHSGRIKRDENVKRGRGRPNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQ97</accession>
    <name>A0A8I6XQ97_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-damage-repair/toleration protein DRT111, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQ97"/>
    <dbReference type="SMR" id="A0A8I6XQ97"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232860"/>
    </dbReference>
    <dbReference type="OMA" id="HACFYDE"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010017">
        <property type="term" value="P:red or far-red light signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000033">
        <property type="term" value="P:regulation of seed dormancy process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010029">
        <property type="term" value="P:regulation of seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010119">
        <property type="term" value="P:regulation of stomatal movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010218">
        <property type="term" value="P:response to far red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd12647">
        <property type="entry name" value="RRM_UHM_SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000079">
        <property type="entry name" value="Putative splicing factor 45"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040052">
        <property type="entry name" value="RBM17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034653">
        <property type="entry name" value="SPF45_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288:SF8">
        <property type="entry name" value="SPLICING FACTOR 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288">
        <property type="entry name" value="SPLICING FACTOR 45 SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031066">
        <property type="entry name" value="Splicing_factor_SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="G-patch" evidence="10">
        <location>
            <begin position="216"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="278"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR031066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="41732" checksum="7A8E50C74F040500" modified="2022-05-25" version="1">MLGGLYGDLPPPTSAAGDDDKPTASVWSSATKMAPPTLRKPSATFAPPTSLLRNQHPRPAAAPKPSAVLQQQQPLASAATTTTVPSFHPALVAVQSTVLEEYDPARPNDYEDYRKDKLKRAKDAEMKKELDRRRREDQEREREREQREAEARLREEQSRASSLNISGEEAWKRRAAMSGAGGGTPQRASSPPHGDAGGFSIPGSSSSGLGLGAGGQMTAAQRMMAKMGWKEGQGLGKQEQGITAPLVARKTDRRAGVIVDESSSRRPRSVNFEGQPTRVVLLRNMIGPGEVDDELEDEIASECSKFGAVLRVLIFEITQANFPADEAVRIFVLFERTEDSTKALVELEGRYFGGRIVHATFFDEGRFERNELAPMPGEVAGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRU5</accession>
    <name>A0A8I6XRU5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnRK1-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123399857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950175.1">
        <property type="nucleotide sequence ID" value="XM_045094240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRU5"/>
    <dbReference type="SMR" id="A0A8I6XRU5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495530"/>
    </dbReference>
    <dbReference type="GeneID" id="123399857"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0410870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0410870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0410870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0495530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399857"/>
    <dbReference type="OMA" id="HHEDHFH"/>
    <dbReference type="OrthoDB" id="1935514at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037489">
        <property type="entry name" value="RAD52-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34050">
        <property type="entry name" value="DNA REPAIR RAD52-LIKE PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34050:SF4">
        <property type="entry name" value="OS09G0512800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20073" checksum="91FA30C0C93EB298" modified="2022-05-25" version="1">MEAAALRPFAAPLGPARRAGVGWRCAPGQRRLGRGARVVARLDGGVGKGVPTTNYVVPLDKATGMTRPLVEILRDLNKRVPDKIIDPDTNTVPWYHANRMLSFYAPGWCGEVRDVIYSNSGTVTVVYRVILKGTDGEAFRDATGTAKVHQGRNDDAVAAAEEAAFSKACARFGFGLYLYHQDEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRZ1</accession>
    <name>A0A8I6XRZ1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123451986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984492.1">
        <property type="nucleotide sequence ID" value="XM_045128557.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984493.1">
        <property type="nucleotide sequence ID" value="XM_045128558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRZ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0505380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0505380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0505380"/>
    </dbReference>
    <dbReference type="GeneID" id="123451986"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0419770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0419770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0419770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0505380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0505380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0505380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451986"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0505380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="144" mass="16050" checksum="9E33952A5016F9EA" modified="2022-05-25" version="1">MDIATYLIQITDLLPLPGPGVEVGILHLVGPQSHARDGHGFSASEFSWMPTQCTSLLNTMSMPWSVVCSSSNVHTLLSMAPVQQQPENNGELILRYVKKGWEDHIKDPGSYWKFRDHREQWTLHTNNLSLVPGGICSSQLPMPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XX19</accession>
    <name>A0A8I6XX19_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XX19"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0538720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0538720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0538720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0538720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0538720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0538720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000465">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000870">
        <property type="entry name" value="OSJNBa0016N04.16-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF76">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="140"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="722"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0538720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1061" mass="119061" checksum="93E3E5253D1FD275" modified="2022-05-25" version="1">MVIGLVIALVALAAGWFWHRRYMSRWQHKMQDLPKRKEIGGHPHRRSLAPISLNPTTIAIGDIASNKGHNTPKQRLQEGIAAPKQVKHMGQIQVIPGTSNATTTPSTASIEKENGQQSYSKVDVPRSFPYEELSQATGNFNENRKLGEGASGAVYEVYGETDNDARPASHLSWLVRHNIIIGIASGLAYLHKECRVVIVHRDLKPANVMLDRNWNAKLVDFGLVTQLSHTQTSRNMDNIRGTRAYIDPAYLESGKVSEQCDVYSLGVVLLEIVCGTKPSILQSSDKHSLIEMVQDCDRSNRILDAADKGLRGQFDKKIEAVLRLGLQCVLPDHHNRPHAGRVRDRLMELLENGKVAFSTPQPSEEQFGTRTVTPVPAASLPAGWFWRGRHKSQRKHKTQGTSTEKENGQQSYSEVNVPRHFTYEELAQATSNFNEYRKLGEGAFGAVYGVHFGTYNNTRPSSPLSWLTRRNILIGIASGLAYLHNECPAAIVHRDLKPANVMLDRNWNAKLADFGLVTQVRHTETSRFTDNVIGTRSYIDPAYMDTGKVSGQCDVYSLGVVLLETICGEKPIILQGGNKNSLIEKVRECDDNNRILDAADKGLKGQFDKEIKAALRLGLQCVSPDNRPHAGRVRDRLTKLLEYGNTSEETKEASTVNLEALSRRCILQGEGSIMATQTYGFDEAPQSAGSMLSSSRDNDMASKLQNTTCLTIRVLQEITDNFSEKRKIGQGACGKVYRAELQNGKEIAVKVLYNNVVMIDDVQFQREFENLMRLEHHNIVRLVGYCYETQHQPIQHMGGIVFAEMTYKALCFEYMQNGSLEEHLSDEGDGLEWHTCYNIIKGAWKGLQYLHKGSDKPIYHLDLKPGNILLDKNMVPKLADFGLSKLMGDQQSIITKTLMGTIGYMPKEYFIGNIVSNKFDIFSLGVVMIKIIAGREGHSKSVDMSRRKFLDLVQRNWRDKMQETCLARRLLEAYCKQVNVCTEIALSCMDTDRHKRPNIVDIIHQLNGTEAVIDRVRSDALTRSEVALGHDTTRASRRGGGDAAHRLGTAWGWRWREQRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y127</accession>
    <name>A0A8I6Y127_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123409715</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044958511.1">
        <property type="nucleotide sequence ID" value="XM_045102576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y127"/>
    <dbReference type="SMR" id="A0A8I6Y127"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0642320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0642320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0642320"/>
    </dbReference>
    <dbReference type="GeneID" id="123409715"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0642320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0642320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0642320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409715"/>
    <dbReference type="OMA" id="PETNTGR"/>
    <dbReference type="OrthoDB" id="264917at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000005">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000058">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000001">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF106">
        <property type="entry name" value="OS06G0126250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035204314" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="24"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="510"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="798"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="811"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0642320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="844" mass="91360" checksum="C41DB3A7B729F819" modified="2022-05-25" version="1">MVRRGALPLALLAALATLTAVSGQGKPVTDNGAGSSQAKFTPKDAFYIDCGGTAAADTKDGKSFKTDAEANNLLSAKDAIKVADDKADVPSHLYRTARVFKAEAIYNFPLTAPGWHFIRLYFFPIKSGEADLAAATFDVTTAANVLLHSFTAEAKAVMKEYVINATENKLELKFTPASGSAAFINAIEVVNAPDQLLNDKALTVSPLAEISGLSEAAYQVVCRLNVGGPVIGPVNDTLGRQWDEDGPYLNPKEAGVEVSVSPNVIKYPETYPVSKLVAPTAVYTTARHMANSGVANQNFNVSWKVDVDASFDYLVRLFFADIISDTMNDLYFNVYINERKAISGLDLSTITGDMAAPFYKDFVVNSSVDTDGHIIFAVGPMGQDTGRIDALLNGAEVLRISNSVGSLDGEFGVDGRMVDDGSGTRKVVAAVGFAMMFGAFAGLGCMVVKWHRRPQDWERRNSFSSWLLPIHTGQSFTNGKGSKSGYTVSSTAGLGHFFSFAEMQEATKNFDDSAIIGVGGFGNVYVGEINDPDEEGSRIKVAIKRGNPSSEQGINEFNTEIQMLSKLRHRHLVSLIGYCDENEEMILVYEFMQHGPFRDHIYGGPEGLPTLSWKQRLEICIGAARGLHYLHTGTAHGIIHRDVKTTNILLDEKFVAKVADFGLSKDGPGMNQLHVSTAVKGSFGYLDPEYFRCQQLTDKSDVYSFGVVLLETLCARAPIDPQLPREQVSLAEWGMQWKRKGLIEKIMDPNLAGKVNPESLAKFAETAEKCLCEFGSDRLSMGDVLWNLEYALQLQEANPPEGATDADDADASIVSSSSGVSTVPDASTTSANELFAQLADMKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1V8</accession>
    <name>A0A8I6Y1V8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay. Functions as a positive regulator of the cytokinin signaling pathway. May play a regulatory role in salt and drought tolerance during plant development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045123604.1"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
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        <property type="term" value="F:protein histidine kinase binding"/>
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        <property type="term" value="P:cytokinin-activated signaling pathway"/>
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        <property type="term" value="P:phosphorelay signal transduction system"/>
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        <property type="term" value="P:positive regulation of cytokinin-activated signaling pathway"/>
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        <property type="entry name" value="AHP1-5/YPD1"/>
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        <property type="entry name" value="HPT_dom_sf"/>
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        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF27">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
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            <end position="134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057097"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="16192" checksum="793FA114214EC830" modified="2022-05-25" version="1">MAAAALRAQLNAHIASMYATGAVDAYFQQLQSMDEGSAATGFVAEVVNIFLNDADRILNNIDGLLNQPAVDFYKVDALVHQLIGSSSTVGAKKVKLACMHFRQFYVAQSKERCHMSLALLRSEFSDVRNKLQTMLQLERQIAALSPR</sequence>
</entry>
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    <accession>A0A8I6Y306</accession>
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        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
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        <name type="common">Domesticated barley</name>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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    <reference evidence="4" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">subsp. vulgare</strain>
        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
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        </source>
    </evidence>
    <sequence length="230" mass="24078" checksum="7FA7278AA4BB15A9" modified="2022-05-25" version="1">MGDVDCELDCGDIDCGECLSVCCSSACDGGGGYGGGAWPYYPYHHHSSGGDRCTCLCYCLLLVILLVLLVVPYFVVFPVHVTVQGASLARLALAGPNGTALAYDVSLAVAVHNRNWASLAKLGAVDAELRFAGARIAGVRMQGEGSSREIEPGKADVYHVAAAGESAQLGGDGVAEFVKESAAGGVFRLELKLSGEVRYPPHTRVNRLEAACPLELPLSSPAFMKKVECV</sequence>
</entry>
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    <accession>A0A8I6Y5N0</accession>
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        <recommendedName>
            <fullName evidence="2">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123397082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045091769.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0503530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503530"/>
    </dbReference>
    <dbReference type="GeneID" id="123397082"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0418230"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503530"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="48"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="691" mass="79437" checksum="4504EAE8BF210F9D" modified="2022-05-25" version="1">MAAWPGELWKRWSIQILVLLSLGLQVALFLFAGIRRRGAHPVLRSLLWLAYQLADYTATFALGHLSLKGAPRDHPIVALWAPFLLLHLGGPDNITAYSLEDSKLWKRHLLHAVLQVSGAVYVLYEHIADRGASLLLASVLMFAIGAVKYGEKTWALMRGNLDSIRGSIEKQPPAMHGHFHPQDRVLKDEELDEESLVRRAHSLFHICKRAVVDYPVMEDDSHGQDTTKMIGEVKLWRLMETELSLMYDTLYTKAPVIHTWFGYSVRLVSPLAIVASLLLFKLIDKDDDNRSVDVYITYVLYGGALFMETTSLLNALGSSWTFAFLSTTRWRWLRYAALCNERWDRLRRVVAYLHHLVRVGGGSRYTSRRWSYTMGQYNMLHFCTRSVTRPLLGRLAKMVGFGLNEVWNREHHSWVIDMPDHVKDCISRHMNKMYKEGAVNSLGMLRNRWGKETLVSNKLFTGIFKDSLGVEFQECIIIWHVCSDVFLAKSDRAKEEEAWLDVEAIRVISNYMMFLLVEQPDMLPGLPQNRMYQRTCENLVKIRRSTHRRQSMNLGATLKNLFRLHDDPESSSRIADREELARNVYDEYESRSFSYDAPRLPYVAQLAKQLLTMEKDGTLDSVKLVLDVWTDILVYAGNKCSRKAHAEKLNSGGELTTILWLMPEHFYQLYLGTLIRRKKMDLGEGISHAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5Y8</accession>
    <name>A0A8I6Y5Y8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123395777</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="SMR" id="A0A8I6Y5Y8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0364340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0364340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0364340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0440160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0440160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0440160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22585">
        <property type="entry name" value="Rcat_RBR_DEAH12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000020">
        <property type="entry name" value="ATP-dependent RNA helicase DEAH12 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001279">
        <property type="entry name" value="ATP-dependent RNA helicase DEAH12 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002114">
        <property type="entry name" value="ATP-dependent RNA helicase DEAH12 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000033">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056245">
        <property type="entry name" value="KH_DEAH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056246">
        <property type="entry name" value="KH_DEAH11/12_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056247">
        <property type="entry name" value="KH_DEAH11/12_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056248">
        <property type="entry name" value="RBD_DEAH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF260">
        <property type="entry name" value="RBR-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24471">
        <property type="entry name" value="KH_DEAH11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24638">
        <property type="entry name" value="KH_DEAH11_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24641">
        <property type="entry name" value="KH_DEAH11_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24475">
        <property type="entry name" value="RBD_DEAH11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="288"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="491"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="1486"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1490"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0440160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1708" mass="191165" checksum="3AFCB6A04684C899" modified="2022-05-25" version="1">MKSELPGEHSVRFQPPTKTFSNPSAAAMRRSPADRPFRPPDRGSLPPHRSQQQRQPQPQSAAILLLRAGPDFSSPAATEVDALVAGLRPDSLSVFSSGRHAARLIFRSLPAAAAAARDLWSLRLEGRHLLTPYTPDPVFAAKAFPLISSLFASHASRLLDSDLVSRLAARSGQLAASVQSVKHRLRISRNSLRDFEQLSLQRKTLEADKDMLDAKIAEYKAAMMSIRRAMLSETDEEKEVDEEEEGVDLFGIVEGEDVDFARVHTMMLRECRRLNEGLPIYAYRRKILNHVFTNQVMILIGETGSGKSTQLVQYLADSGLAAEGTIFCTQPRKIAAITLAHRVDEESNGCYAHNFVSSCSTFPGSRGFSSKIIFGTDSYLLHHCMNDMGLDGVSYIIVDEAHERSLNTDLLLALIKKKLLDRVDLRLIIMSATADANRLAEYFYGCQTFHVKGRNFPVEIKYIPDVSVDASFNTVPNSVNDACATASYVNDVVRVVGVIHKNEEEGAILAFLTSQLEVEWACENFSDASAVVLPMHGKLSHVEQSRVFNSYPGKRKVIFCTNMAETSLTIKEVKYVVDPGLAKESRFVPSSGLNVLKVNWISQSSANQRAGRAGRTGAGKCYRLYSESHFRMMEVHQEPEIRKVHLGTAVLRILALGVRDAQNFEFVDAPNPEAINMALKNLEQLGAIKCKCNLFELTDTGRYLVKLGIEPRLGKIMLDCFDSGLRKEGVILAAVMANSSSIFCRVGTNEEKHKADLQKVRLCHQDGDLFTLLAVYKKWEDGHGNRNLWCWQNSINAKTMRRCQETISELENCLKHELNIIVPSYWCWNPEVPSMHDKLLKRIILSTLTGNLAMFLGHEKFGYQVISTDQAVNLHPSCSLLNYGIKPEWVVFTEILSVPNQYLVCVTAVDCDELYAIHPMSFIQQLEKHKLQIKVISGLGRALLGRFCGKYGQNQQRIISRLKEECRDDRITVEINFENNEVVLFATKQDIEKVFCIVNGVLECEGKMLRNECLQKSLFPSRIGSSPLALFGSGAEIKHLELGKRYLTVEVLHQNACDINDKELIFLVDSLISGVANFHKSSGNFRIASDETKWGKFTFLKPEGAEEAVSKLNGMEFHGSSLKVVPVCSDNRGLPFPAVRGRLSWPCNPNRGRALGTHLLRLVVPSVSACEEALVREISSFMPNKSFPGQNFRVEVFPPEENNSMMRATITFDGSLHREAARALDHLEGSFLPCCQPWQIIRCKHVFHSTLSCPLRVYNVISQEVASLLESFRSQKGVSYNFEKTENGIFRLKLTANATKTIADLRRPLEILMKGNTVNHPDLTLSAVQLLLSHDGVAHLKSIEQETGTYMLYDRQSLNIKVFGHQDQMAAAETKLVHALTELLEKKPLEISLRGHNLPPDLMKKTVENFGVDLEGFKKEMPAVNVELHKHRHLLKVWGSKEDKRRVEGMISELITSVKQNALVQLPPGSVGGSKDKQRVDDNELAQDACPICLCETEDPFELESCGHMFCRACLVDQCESATKSHDGFPVCCLKTGCKKPFLIVDLKHLVSNEKLEDLFRASLRAFVASRAGTYRFCPTPDCQSIYQVAAPDAETKPFVCGACYVEICTKCHLEYHPFMSCEAYKQYKEDPDATLLEWRRGKGNVKNCPSCGYTIEKSEGCNHVECRCGSHICWACLANFRSSEECYGHLRMMMMFVCLYINLAVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6B6</accession>
    <name>A0A8I6Y6B6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123452302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984879.1">
        <property type="nucleotide sequence ID" value="XM_045128944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6B6"/>
    <dbReference type="SMR" id="A0A8I6Y6B6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0511660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0511660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0511660"/>
    </dbReference>
    <dbReference type="GeneID" id="123452302"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0424990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0424990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0424990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0511660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0511660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0511660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452302"/>
    <dbReference type="OMA" id="RPRAKWI"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000108">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1412">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 83A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0511660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="50294" checksum="773EFDD0658B03CD" modified="2022-05-25" version="1">MAKGHVLVLPMPCQGHVVPLMELSHRLVDHGFEVTFINTDVDHALVLAALPEGVEALRGIHLASIPDGLADDEDRKDLNKLVDAYPRHMPAYLEALIGDMEAAGRRRVKWLIADFNMGWSFEVAKKLGIRCASFWPASAACLAIMLNIPKLIQDGVLNDKGWPDREETLQLAPGMPPLHTSLLPWNSAGAPDGQHIIFQLVCRNNKFNDHAEMTVCNSFHEAEAGAFKLFPNILPIGPLFADQRSVGSFLPEDTSCLKWLDAWPDGSVVYVAFGSMAIFDSRQFQELAEGLQLTGRPFLWVVRPDFTAGLSKEWLEEFQKHVAGTGMIVSWCSQQQVLAHRSVACFVSHCGWNSTMEVVRNGVPVVCWPYFCDQFLDRSYVTDVWRTGLAVSTGEDGVVTKEEVRCKVESVVGDAEFRNRARWLKDNAWRCIGEGGSSHENFTRFVDLLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y722</accession>
    <name>A0A8I6Y722_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123402190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952005.1">
        <property type="nucleotide sequence ID" value="XM_045096070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y722"/>
    <dbReference type="SMR" id="A0A8I6Y722"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620720"/>
    </dbReference>
    <dbReference type="GeneID" id="123402190"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0514900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0514900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0514900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402190"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000003">
        <property type="entry name" value="Ribonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF75">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035292313" evidence="9">
        <location>
            <begin position="26"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25000" checksum="CA96550D0C5025A0" modified="2022-05-25" version="1">MMRPCCLSLALLVALSAVGCAAAQAADYDFFYLVLQWPGSYCDTKKSCCYPRTGKPAADFGIHGLWPNRDDGSYPQNCNPANAFDPSKVSDLLSSLRAEWPTLACPASDGLQFWAHEWEKHGTCAQNLFNEHGYFQTALHLRDQLRVLDALASAGVAPDGGYYTLSAVKGAIQQGTGFEPFVECNRDESGNSQLYQLYFCVDARASGFVECPVQPGGRPCGDRIEFPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7N3</accession>
    <name>A0A8I6Y7N3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17 18">Plasma membrane ATPase</fullName>
            <ecNumber evidence="3 18">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123402935</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="3 18"/>
    <dbReference type="RefSeq" id="XP_044952839.1">
        <property type="nucleotide sequence ID" value="XM_045096904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7N3"/>
    <dbReference type="SMR" id="A0A8I6Y7N3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624610"/>
    </dbReference>
    <dbReference type="GeneID" id="123402935"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0518380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0518380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0518380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0624610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0624610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0624610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402935"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 18" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="241"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="646"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="711"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="754"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="785"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="818"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="15"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0624610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="950" mass="104245" checksum="A6F0924A4390026E" modified="2022-05-25" version="1">MASMTLEDVKNETVDLETIPVPEVFSHLKCSKQGLSGTEAQNRLAIFGPNKLEEKTENKLLKFLGFMWNPLSWVMEAAAIMAIVLANGGGKPPDWQDFVGIVTLLFINSTISFIEENNAGNAAAALMAGLAPKTKCLRDGKWSEMDASFLVPGDIISIKLGDIIPADARLLEGDPLKVDQAALTGESMPVNKHSGQGVFSGSTVKQGEIEAVVIATGVHTFFGKAAHLVDSTNNVGHFQQVLTAIGNFCIISIAAGMLVEVVVMYPIQHRAYRDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHRLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLTVDKTLIEVYGRGIDKDTVLLYAARASRVENQDAIDTCIVGMLADPKEARAGIQEVHFLPFNPVEKRTAITYIDGNGDWHRISKGAPEQIIELCRMPKEAEKRVHGLIDQYADRGLRSLGVSYQPVPAKNKDSPGEPWQFVGLLPLFDPPRHDSAETIRRALHLGVNVKMITGDQLAIGKETARRLGMGTNMYPSTTLLGDKSTEMSGLPIDELIEKADGFAGVFPEHKYEIVKRLQDRKHICGMTGDGVNDAPALKKADIGIAVDDATDAARSASDIVLTEPGLSVIVSAVLTSRAIFQRMKNYTIYAVSITIRIVVGFMLVALLWKFDFAPFMVLIIAILNDGTIMTISKDRVKPSPTPDSWKLKEIFATGVVLGTYMALVTVLFFYLAHDTEFFPETFGVRSIRENEKEMMAALYLQVSIISQALIFVTRSRSWSFVERPGALLVIAFFVAQLLATCIAVYANWEFCKMQGIGWGWGLSIWAFTVVTYIPLDILKFIIRYALSGRAWNNINNKTAFTNKNDYGKVEREAQWATAQRTLHGLNQGSNNSDLFADNNGYRELSEIAEQAAKRAEVARLRELHTLKGHVESVVKLKGLDIETINQSYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9X5</accession>
    <name>A0A8I6Y9X5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATPase inhibitor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase inhibitor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9X5"/>
    <dbReference type="SMR" id="A0A8I6Y9X5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0689440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0572480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0572480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0572480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0689440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0689440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0689440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="At2g27730-like"/>
    </dbReference>
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        <property type="entry name" value="ATPase_inhibitor_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33878:SF4">
        <property type="entry name" value="OS08G0558900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33878">
        <property type="entry name" value="OS08G0559000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04568">
        <property type="entry name" value="IATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0689440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9583" checksum="186C1D77EF484147" modified="2022-05-25" version="1">MAMRCALTSLSARLQSPAVVASGTRRLLSDGKGRVLSEEERAKENVYIQKMERERQEKLKKKLEQEKGEAEKAKSGADGKVYDTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YB19</accession>
    <name>A0A8I6YB19_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123397664</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948135.1">
        <property type="nucleotide sequence ID" value="XM_045092200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YB19"/>
    <dbReference type="SMR" id="A0A8I6YB19"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0498720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0498720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0498720"/>
    </dbReference>
    <dbReference type="GeneID" id="123397664"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0413870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0413870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0413870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0498720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0498720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0498720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397664"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000317">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase At3g15890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000406">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase At3g15890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF7">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="59"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0498720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="386" mass="43015" checksum="74D4B0320621F58D" modified="2022-05-25" version="1">MINRCLCCVAPGGDSEPEAAPSSRRRDPSRRGSKNKNRSVEFPWEMYTLKELLQATNNFNESNKLGEGGFGTVYWGRTSKGVEIAVKRLKAMTAKAEMEFAIEVEILGRVRHKNLLSLRGFYAGGDERLIVYDYMPNHSLLTHLHPHRGTPASQQHPPLDWPRRLAIALGAAQGLAYLHHEASPHIIHRDIKASNVLLDADLVPKVADFGFAKLIPEGVSHLTTRVKGTLGYLAPEYAMWGKVSESCDVYSFGVLLLELISARRPLEKLPGGVKREIVQWAGPLVERRKWDRLADPRLAGRFDAVQLRAVVETAMLCSQSNAEGRPTMAEVVEMLKFGGERRNREIVPVADAASEDTTVTMDREDDVTGSSEPLDRGRSWKLTTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGU0</accession>
    <name>A0A8I6YGU0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123452055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984564.1">
        <property type="nucleotide sequence ID" value="XM_045128629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGU0"/>
    <dbReference type="SMR" id="A0A8I6YGU0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480680"/>
    </dbReference>
    <dbReference type="GeneID" id="123452055"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0398380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0398380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0398380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452055"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF674">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035292316" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40010" checksum="8F233AF5CDEF88D7" modified="2022-05-25" version="1">MELTLVFSLLVLFCLHGVSADRPNFTSLFALGDSNTDTGNLVIMATPDVPVFNNKPPYGMTFFGHPSGRFSDGRVTIDFIAEKFGLPLLRPSLQNNSDVSKGVNFAVGGATSINADFFERNKLVNFKLLNNSLNVQLDWFEKLKPSFCKTAGPSECFNKTLFFVGEFGVNDYNLAWGAGKAESEVRSYVPQVVQNIANAAEVLIKGGATYVVLPGIPPIGCSPSLLATRVRLNKATEFDQIGCLSDVNRVAKYHNTELRDAIGGLRGKYAHAKVIKADFYDPIIEILQNPGRFGVAGGDVLRACCGGGGKYNWNVTAVCSMPGVAACKDPSAFVSWDGTHFTEATYRYIAKGWLSGPYSDPPILNALNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIF4</accession>
    <name>A0A8I6YIF4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123411376</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044960249.1">
        <property type="nucleotide sequence ID" value="XM_045104314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIF4"/>
    <dbReference type="SMR" id="A0A8I6YIF4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0645480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0645480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0645480"/>
    </dbReference>
    <dbReference type="GeneID" id="123411376"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0535850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0535850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0535850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0645480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0645480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0645480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411376"/>
    <dbReference type="OMA" id="HATWPKI"/>
    <dbReference type="OrthoDB" id="419694at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237224" description="2'-phosphotransferase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0645480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="285" mass="31220" checksum="DB5E88498FE358E4" modified="2022-05-25" version="1">MRALTAATISSPTRALLLLHLSFPSTLRLPLTMNPSSSSGYRSNAAASASTHQPRGGRGGARRPGGRGGGGGGGDGRDRIDALGRLLTRVLRHMAPELRLDMRSDGYVRVRDLLRLNLQTFAKVPLNSHTVDEIREAVRRDNKQRFGLLEEDGELLIRANQGHTVTTVTSESLLKPILSADEISVCVHGTYRKNVDSILKYGLKRMARLHVHFSSGLPSDGGVISGMRTSANILIYLNVRKALQDGMKLYISENKVILTEGFDGVIPVKYFEKIETWPGRAPIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJK5</accession>
    <name>A0A8I6YJK5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123407313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956354.1">
        <property type="nucleotide sequence ID" value="XM_045100419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJK5"/>
    <dbReference type="SMR" id="A0A8I6YJK5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0716830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0716830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0716830"/>
    </dbReference>
    <dbReference type="GeneID" id="123407313"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0594640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0594640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0594640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0716830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0716830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0716830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407313"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044658">
        <property type="entry name" value="bHLH92/bHLH041-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055477">
        <property type="entry name" value="DUF7049"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055478">
        <property type="entry name" value="DUF7050"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46665:SF16">
        <property type="entry name" value="OS06G0570900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46665">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH041-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23132">
        <property type="entry name" value="DUF7049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23133">
        <property type="entry name" value="DUF7050"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BHLH"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="324"/>
            <end position="373"/>
        </location>
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        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
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        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0716830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="525" mass="55558" checksum="EDC767EFF865BB05" modified="2022-05-25" version="1">MDSVFALAAVPRALVLERAAARVPGCLYLCLWAPVTAQLPSSHLFCLDAWIAGGGGGARARAGFEAYRGALCAVVSGCVPGWAYREGRAYMELPEPDLTSSASLQVQQQFYHEAGTKMAVFMGCESGEIEIGLSTTSVAAAAVADHVHQSFLEDLLQLPPAGLSSSSSPVPSLSIGSPEYSSLIRAMATPAATAGEPSTQPLLMHASSLPGLLAPIVEANDYALMAQAMLAAIPSPGASTTSTPPPPPCPPWLASHRSQRSSPRRTTAFKPYRAALSPRARPRPGAPGQRMIKTCMSLLASVHMSMRNQPELAGAHHEPPPSSTSQLHHVISERRRRERLNDSFQTLRALLPPGSKKDKANVLASTTEYMAKLVSQVTQLRERNLQLEAELGLNPSASDINNPSGKTVEVEVTTGASTSTSTSSTTPSQQPREVGVRVTVRAECDMSDMLVSLLARLKDAGGFMVVAAEARQQSSALARASLTLRMTEAGDVVDATRLEEALTKVVEDAVMKTPPPPVVPPPRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKZ4</accession>
    <name>A0A8I6YKZ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Circadian clock associated 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123401551</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044951312.1">
        <property type="nucleotide sequence ID" value="XM_045095377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKZ4"/>
    <dbReference type="SMR" id="A0A8I6YKZ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0604730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0604730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0604730"/>
    </dbReference>
    <dbReference type="GeneID" id="123401551"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0501340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0501340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0501340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0604730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0604730.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123401551"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein REVEILLE 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
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    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF178">
        <property type="entry name" value="OS02G0685200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="47"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="47"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="50"/>
            <end position="101"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
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            <end position="219"/>
        </location>
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            <end position="409"/>
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        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0604730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="48572" checksum="EBE751BC39675799" modified="2022-05-25" version="1">MACTQLQEKAAMATDESTADHRRNRPSSHDMDLSGDDHVPKARKPYTITKQREKWTEEEHKRFLEALQLHGRAWRRIQEHIGTKTAVQIRSHAQKFFSKVTRESSGSSSGSGAAAATPTAAIQIPPPRPKRKPAHPYPRKADDGKHVQGLTRLERPPVRMCEQEEGSPTSVLTASRVEASGGGFSNNSSSSRSPAPSAAGSLYGSSVDRGDGCLSPNTKFQASEFTANGDAKEGSCTGSETSVLKLFGKKVVVNDTYQKPNTSTGNLQNGGDVGTEASDDTTTQGSRNLPSGGATEGSSWTPWPSSMQQFVYFVPQPDGFTTQSAMVPWFGTLPGAMFYQQAVAPNQHQRHRSETADNKFMQREGSWTGSNTGPGSAVHNSDAADSRGRGNSSESDKTPLPRLTKCESSVSVSLQRGFMPYKRCSAESESLRSEAPREETDGELTRLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKZ9</accession>
    <name>A0A8I6YKZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="3 12">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123452731</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="3 12"/>
    <dbReference type="RefSeq" id="XP_044985376.1">
        <property type="nucleotide sequence ID" value="XM_045129441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKZ9"/>
    <dbReference type="SMR" id="A0A8I6YKZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519720.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519720.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519720"/>
    </dbReference>
    <dbReference type="GeneID" id="123452731"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0432020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0432020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0432020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519720.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519720.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519720"/>
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        <property type="component" value="Chromosome 5H"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
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        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Lysine--tRNA ligase"/>
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        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lys_tRNA_synth_class2"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="NA-bd_OB_tRNA"/>
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        <property type="entry name" value="PRK00484.1"/>
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        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRNASYNTHLYS"/>
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    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="275"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00008226"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
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            <dbReference type="RuleBase" id="RU003748"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519720.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="606" mass="68331" checksum="0176B1FF5785360D" modified="2022-05-25" version="1">MADGADGLAERLAGAEIAADGGGGEEEPKLSKNAQKKEEKRKKQEEQRRLKEEEKKNKAAAAAASAGGAPQQAAAADDEDMDPTQYYENRLKTLDTLKAAGANPYPHKFLASISVPGYIEKYKGLNEGEKLADVTECLAGRIMNKRTSSSKLLFYDLYGDGVKVQVMADARTSELEDAEFSSFHSGVKRGDIVGICGIPGKSNRGELSVFPRKFVVLSPCLHMMPRQKNEASAVHTPWIPGMCRNIEKYVLRDQETRYRQRYLDLMVNHEVRHIFKTRSKIVSFIRRFLDGLDFLEVETPMMNMIAGGAAARPFVTHHNDLNMKLYMRIAPELYLKELVVGGLDRVYEIGKQFRNEGIDLTHNPEFTTVEFYMAYADYNDLMELTEAMISGMVKELTGGYKIKYHANGVDNPPIEIDFTPPFRRIDMIEGLEAMAKLEIPKDISSDETNKYLIDACAKYDVKCPPPQTTTRLLDKLVGHFLEETCVNPTFIINHPEIMSPLAKWHRSRPGLTERFELFVNKHEVCNAYTELNDPVVQRQRFEEQLKDRQSGDDEAMALDETFCTALEYGLPPTGGWGLGIDRLTMMLTDSQNIKEVLLFPAMKPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMJ5</accession>
    <name>A0A8I6YMJ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">monodehydroascorbate reductase (NADH)</fullName>
            <ecNumber evidence="9">1.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123411245</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2 monodehydro-L-ascorbate radical + NADH + H(+) = 2 L-ascorbate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:14581"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59513"/>
            <dbReference type="EC" id="1.6.5.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.4" evidence="9"/>
    <dbReference type="RefSeq" id="XP_044960114.1">
        <property type="nucleotide sequence ID" value="XM_045104179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMJ5"/>
    <dbReference type="SMR" id="A0A8I6YMJ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0688870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0688870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0688870"/>
    </dbReference>
    <dbReference type="GeneID" id="123411245"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0571990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0571990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0571990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0688870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0688870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0688870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411245"/>
    <dbReference type="OrthoDB" id="432169at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016656">
        <property type="term" value="F:monodehydroascorbate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000013">
        <property type="entry name" value="Monodehydroascorbate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000155">
        <property type="entry name" value="Monodehydroascorbate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048618">
        <property type="entry name" value="MDHAR3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF17">
        <property type="entry name" value="MONODEHYDROASCORBATE REDUCTASE 4, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21791">
        <property type="entry name" value="MDHAR3-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="7"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Monodehydroascorbate reductase 3-like C-terminal" evidence="12">
        <location>
            <begin position="345"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0688870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="435" mass="46561" checksum="24C306CCC066EE29" modified="2022-05-25" version="1">MASEKHFKYVILGGGVAAGYAAREFGKQGVQPGELAIISKESVAPYERPALSKGYLFPQNAARLPGFHTCVGSGGEKLLPEWYTQKGIELILSTEIVKADLASKTLTSAAGATFTYETLLIATGSSTIKLTDFGVQGAESNNILYLREIDDADKLVAAMQAKKDGKAVVVGGGYIGLELSAALKINNFDVTMVYPEPWCMPRLFTAGIAHFYEGYYASKGINIVKGTVASGFDADANGDVSVVKLKDGRVLDADMVIVGVGGRPLTGLFKGQVDEEKGGLKTDAFFETSVAGVYAIGDVATFPMKLYDEPRRVEHVDHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMP6</accession>
    <name>A0A8I6YMP6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC123395045</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="RefSeq" id="XP_044945898.1">
        <property type="nucleotide sequence ID" value="XM_045089963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMP6"/>
    <dbReference type="SMR" id="A0A8I6YMP6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0532650"/>
    </dbReference>
    <dbReference type="GeneID" id="123395045"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0443000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0443000.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0443000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0532650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0532650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0532650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395045"/>
    <dbReference type="OrthoDB" id="619632at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000302">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000027">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF4">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237145" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="29"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="23"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="286"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="336"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="525"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="812" mass="90171" checksum="06423257833B3DC7" modified="2022-05-25" version="1">MAPALALVLATTSLFCLSTLLLVPRAVAYDILPLGSSLTVESFQTNILQSPDGTFSCGFYSIYTNAFTFSIWYSKAANKTVVWSANLDRPVHVKRAAFTLQKDGNMVLKDYDGAAVWKAGGNSTNVQHAQLLDTGNFVLKDTDGKIIWQSFDSPTDTFLPTQPIKVSTKLVSTTRSRVPGKYIFRFSDLSILSLIYDIPEVSDIYWPDPDNSPFQNNRNQYNNTRLGILDSNGSFGSSDFADGQTLVASDAGPGIKRRLTLDPDGNLRLYSLNDSDGSWSVSMVAMSQPCNIHGVCGPNGICHYSPKPTCSCAPGYVMSNPGNWGEGCRAIVNITCDHQEPLKFVKLKNTDFWGSDQKHIPSVSFDACRNICMADCTCKGFQYKEGDGLCYTKASLFSGWTYPINDPRTIYLKLPARVNVSNTNFPYSDVFHSAPPHLDCSQMNTPPILEVHNSNTGVEESKWLYFYGFIVAFFVVEVSFIAFAWFFVLRRELRPSEVWAAEEGYKVMASHFRRYSYRELVKATTDFKVELGRGSSGVVYKGVLEDERPVALKKLKHISGGKEEFQAELSVIARIYHMNLVRIWGFCSEGSHRLLVCEYVENGSLASILFGDKCGILLGWKQRFNVALGVAKGLAYLHHECLEWVIHCDVKPENILLDQNFEPKITDFGLAKLLNRGGSTQNTSHVRGTAGYIAPEWISGRPITSKVDVYSYGVVLLELLSGSRVSELTVGSDAEVHMVLRKLVTMLADKLEGNDDSWIDEFVDCKLGGQLSYVQARTLIKMVVSCLEEDARKRSTMESVVQTLLSSDGADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRI4</accession>
    <name>A0A8I6YRI4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961003.1">
        <property type="nucleotide sequence ID" value="XM_045105068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734770"/>
    </dbReference>
    <dbReference type="GeneID" id="123412122"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0609520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0609520.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0609520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0734770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412122"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="10570" checksum="78C2B5B599B3E10B" modified="2022-05-25" version="1">MEFTLTSTATEVLDKAAALAAAGLGLEGEALGAWGIEEKATAIRRWAKKLSEMEKAAAPVPPEVAGSVPRAEEIGSDVNHVGDVKRDGVDPSISAAVKDSAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZ08</accession>
    <name>A0A8I6YZ08_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123412068</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960954.1">
        <property type="nucleotide sequence ID" value="XM_045105019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZ08"/>
    <dbReference type="SMR" id="A0A8I6YZ08"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731430"/>
    </dbReference>
    <dbReference type="GeneID" id="123412068"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0606910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0606910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0606910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0731430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0731430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0731430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412068"/>
    <dbReference type="OrthoDB" id="1839773at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF136">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH133-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="218"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="384" mass="40639" checksum="AC13952CD95A56AF" modified="2022-05-25" version="1">MDRGLVHGSSPLVGEMGEGHGWRSMNNLRPPFEQNNPSVSLASTSTSICSSPINSFSSLLLSNHYPLPTNSTAPWHDSSNSNQVLQLQDPWNHFMATNGQLANDEVRHKALEGQALFPATTTGEGEEGDGSSAAGHIYSNTARHGSSLAGDEFQLAMSSPSPWSRGVQHYHNTLQQQASSITSTTSLGNNMLDFSNNGSPRECTSTASGAAFKKARTQEPSPAQSTVKVRKEKLGDRITALHQLVSPFGKTDTASVLLEAIGYIRFLHGQIEGLSSPYQPGGGGGGDGGGGSGGSKQRHQASVQQRERSSIFPEEPGQLLHGNATRKRGAPDQEDGDGGEEAKNDLRSRGLCLIPLSCAPDFGADVGPADYWTVAPPFGMGFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z8E7</accession>
    <name>A0A8I6Z8E7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123412851</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961731.1">
        <property type="nucleotide sequence ID" value="XM_045105796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z8E7"/>
    <dbReference type="SMR" id="A0A8I6Z8E7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665410"/>
    </dbReference>
    <dbReference type="GeneID" id="123412851"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0552060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0552060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0552060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0665410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412851"/>
    <dbReference type="OMA" id="EPCNNTT"/>
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        <source>
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</entry>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Fiebig A."/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        </location>
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        </location>
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            <begin position="194"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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        </lineage>
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                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
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        <source>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961828.1">
        <property type="nucleotide sequence ID" value="XM_045105893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3J4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066230"/>
    </dbReference>
    <dbReference type="GeneID" id="123412932"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0052910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0052910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0052910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0066230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412932"/>
    <dbReference type="OMA" id="DYMNDIT"/>
    <dbReference type="OrthoDB" id="1163429at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF285">
        <property type="entry name" value="OS01G0730200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="115"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="48962" checksum="23646D5D8185C760" modified="2022-05-25" version="1">MDHGSSPTKVRKSVVDEDFISNLPEALKDKILCCLPIKEAVGTCLLSRSWRYTWASMTELMFRRADFASGNGNADDDTCRFLKFTDMFLFLHNGPILKFGLNTIENEMVSTGGHLSRWMLMLSRNKIKEIQLWTSIRDSYKIPSCFFSCHELEYVYLRACFFTSLQLPPLSKGFKQLRTLRLEYVSVQGSSLEDLVASCPNLEKLSICRLCSKSNINIRSTKLKMLTIEGRFIHLNLHAPYLTSVWIGLELKSDTDGALTARCNFNLSPFIASLSDVETIRFHGQILECVEHEFLILKQPRLFNRLTEISLEINLGNLKQANFALSLFQHAPNLRLVKLKFISRNSVVPTVHFWESLNRDVCLFQNVDVVSLFNFTGSFAELAFLKLLLEDAPVLRKVRISDKGLDKDVLKNLLMMRRASRDAEILIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4U3</accession>
    <name>A0A8I7B4U3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123424657</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with importin subunit beta-1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044964243.1">
        <property type="nucleotide sequence ID" value="XM_045108308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4U3"/>
    <dbReference type="SMR" id="A0A8I7B4U3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110420"/>
    </dbReference>
    <dbReference type="GeneID" id="123424657"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0090510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0090510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0090510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424657"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="527"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="563" mass="61143" checksum="AF64F56DD11A00CA" modified="2022-05-25" version="1">MPRVPRRPSDEARRGAYKPPGVDAARSRRRREDRLLALRRRNRDAGLFKRRREEPSLAPAPVAPPSDAAADATAPPPPPGPSSAPSSPPPADALRAAADAELEGLSEMVDKVWSDDTTSQLEATVQFRKLLSDGKNSTMIKIIRADVLPRFAEFLSRQRLPQLQMEAAWVLTNIAASDYTLLVAECGAVPRLVELLDSPNANIRHQALWALGNIAADMPSCREIVLDHGAVTPLLAQFKEDMKVSVLRTATWALSNLCFGKLPAEVQVKPILEIISLLIHSADEKILADACWALYYICDGVEGGIQDALDAGVCPQLVKLLMHASANILLPVITSLARISAGDDTQVQVIIENGILPCLTQLLARNYPKIIKKQTCLIVSNITAGSKDQIQAVIDADVMNHVIVLLKTSETDLKKEAAWAISNAASGGSSDQIQYLVSRGCLEPLCNVLKYQDVDLVYTCLEGLQNILEEGETGRQGNECATNPYAQFILENGGLDNLEDLQDFDNDAVYKLAMKLLERYWDEEVSDEANLTASKDSPAENVEAVPEDAAPPPVAAPSVDGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5B9</accession>
    <name>A0A8I7B5B9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger-XS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5B9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0266220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0266220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0266220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0266220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0266220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12947" checksum="FDF3681FCA0DF20A" modified="2022-05-25" version="1">MKRKLDKTGFKSPYDSDYDDDVYEYNCGDDVEDDNKEYFIEKESQKIREKGKVTYFKQGKFMCPYCTTKTKPKDVLYEHLMSHARRLSTNAYDIKIRAEHASLLKALGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B664</accession>
    <name>A0A8I7B664_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B664"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0164730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164730.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="168" mass="19106" checksum="F64BC7822E0592C3" modified="2022-05-25" version="1">MKKKMYILVFTVEGFDQVGDGFDEDDDDPDLDVEGEDKLDDESDLNKDFMEGDNDEPIDELDKANFSKSKSSGSGSKVNETCVSAVKFHPMLLIDEMHEQFIEEAGSKTPPVHVEDIPETAGVISDKEDLDIHTHMEYCSAQHKNFEMDVSEEEDDINYLRWCAFLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8L9</accession>
    <name>A0A8I7B8L9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8L9"/>
    <dbReference type="SMR" id="A0A8I7B8L9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0214150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0178050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0178050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0178050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0214150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214150"/>
    </dbReference>
    <dbReference type="OMA" id="RGMPMVG"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047259">
        <property type="entry name" value="QUIRKY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31425:SF52">
        <property type="entry name" value="MULTIPLE C2 DOMAIN AND TRANSMEMBRANE REGION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31425">
        <property type="entry name" value="PHOSPHORIBOSYLANTHRANILATE TRANSFERASE ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035297009" description="C2 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0214150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="211" mass="23897" checksum="274D79450CA95D4B" modified="2022-05-25" version="1">MRTCKLIITVELALGLIPRDGHGSPSAYVELCLNGHRIRTTIKEEDRHPVWNETFWFDVSPHPSNIHDLVIEASVYNTKEPIQGPGKYFLGMVTRNGDSFRRLCEPTQPCITQQAAHYPLERRRGMPMVGVRGELLLNGYILDETPIRDPSMSSAPTGLCVEVTNAIKLKPMDYLTVVTKTSSNNFIELVRSFVLLTFKNACLRFINTMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BBP4</accession>
    <name>A0A8I7BBP4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123447809</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044980425.1">
        <property type="nucleotide sequence ID" value="XM_045124490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BBP4"/>
    <dbReference type="SMR" id="A0A8I7BBP4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0343380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0343380"/>
    </dbReference>
    <dbReference type="GeneID" id="123447809"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0286590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0286590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0286590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0343380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0343380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0343380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447809"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000028">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="74"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="240"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="387" mass="41939" checksum="1FD21BC969F07496" modified="2022-05-25" version="1">MSLSMGACPHAWQHHLPHRGRMWAASPAFRRQLFLLRSLAPTCVDGGRVSSSSLRLHAMSAAPAAAGPVYEADAEAVVRRITPPLDRARHKGQAGKIAIIGGCREYTGAPYFAAISALKVGADLSHVFCTKDAATVIKSYSPELIVHPILEESYSVRDDERESVSSSILTEVIKWMERFDCIVVGPGLGRDSFLMDCVSNIMRHARQANIPTVVDGDGLFLITNNIGLVEDNSLAILTPNVYEYKRLVQKVLNCEVNEENASEQLTALCEKIGGVTIMRKGKADLISDGKKVTQVSTFGSPRRCGGQGDILSGSVAVFSSWAWHFLSSNEQPKENSVNPMVLGCIAGSLLLRKAAALAFEKNKRSTVTTDIIEFLGQSLEDICPAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCT6</accession>
    <name>A0A8I7BCT6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123449280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982369.1">
        <property type="nucleotide sequence ID" value="XM_045126434.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982370.1">
        <property type="nucleotide sequence ID" value="XM_045126435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCT6"/>
    <dbReference type="SMR" id="A0A8I7BCT6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395620"/>
    </dbReference>
    <dbReference type="GeneID" id="123449280"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0329140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0329140.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0329140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0395620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449280"/>
    <dbReference type="OrthoDB" id="612216at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
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    </dbReference>
    <dbReference type="InterPro" id="IPR055312">
        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF42">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
        <property type="entry name" value="OS10G0396666 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="21"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="308" mass="34012" checksum="32619E5F1F289912" modified="2022-05-25" version="1">MESDEAAARFLVAVPGGGGGSDFLSRLPDHLLVDILHRLCTKEAVATTVLSRRWRAVWAGLPHLNFDDVDPSAPARALASYQDLAAFEIQRLAVSPNQVDAQETSAWLALAAPLLSGPLDLNISRALSKEALDNLLMGGGDGDQEAVEFPCFERATEVRLDLAFLAPALPPVGVFHALRSMFLHNFRCQGQIILSAMMLPSLQHLTMNRGRCLNVLINSEFLICVHLSYLMLLQQLAIVAPGLQQLEVVHCFYDAPIPVANIDAERLQVLSWEVPYDAEFVHLGEMPCLWRLRAPPISMFRWQAIQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BD47</accession>
    <name>A0A8I7BD47_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BD47"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0646860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0646860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0646860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0536810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0536810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0536810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0646860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0646860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0646860"/>
    </dbReference>
    <dbReference type="OMA" id="NNEATME"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34591:SF52">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34591">
        <property type="entry name" value="OS03G0653100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0646860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24968" checksum="42F4DBD7079E48C7" modified="2022-05-25" version="1">MRISLSNSTYRAIRLPTEDAEFYLGKSVNGIYCASLFEGSQLQVWFLNDPCVDGQTEWVLKHDRDIFSILPNLNYDKHRDGPWILQEFHYWGNKDADDDSPIVYNNEATMEEKFVWDSDNDNVLEPGSRSKGCYIAFLGFHPYKQAVFLSDKFDRVLAYNWNSSKLQDLGKVFPKFYIDWDYYYDHKYVEESFPYTPCFLGELPEKINLEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFW5</accession>
    <name>A0A8I7BFW5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter (TC 2.A.67.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0531580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0531580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0531580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0531580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0531580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0531580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0531580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40150" checksum="676EBF3BEBF5D2F1" modified="2022-05-25" version="1">MGAVSKLMDARFRASHQEEPDIHTKLMRKYDDIPTWWFYTLTVLSMTVSLILCTVLKDQVQLPWWGLLFACGMAFVFTLPISIITATTNQTPGLNVITEYAMGLIVPGYPIANVCFKVYGYMSMLQAVAFLSDFKLGHYMKIPPRSMFIVQVVGTLVAATINLSVALWLLGSVENICQDQLLSPDSPWTCPGDRVFFDASVIWGLVGPKRIFGPHGNYEALNWFFLVGAAGPVMVYIFHRIFPSQKWILMINLPVLIGATAYMPAATAVNYNSWLLIGTIFNFFVFRYRKTWWQRYNYMLSGALDAGVAFMAVLLYFSLTMENRTVDWWGTAGEHCPLASCPTAKGVDLGPDSACPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F2CPW6</accession>
    <name>F2CPW6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123443927</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="AK353668">
        <property type="protein sequence ID" value="BAJ84887.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976425.1">
        <property type="nucleotide sequence ID" value="XM_045120490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CPW6"/>
    <dbReference type="SMR" id="F2CPW6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273810"/>
    </dbReference>
    <dbReference type="GeneID" id="123443927"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0226900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0226900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0226900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0273810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443927"/>
    <dbReference type="HOGENOM" id="CLU_011276_7_4_1"/>
    <dbReference type="OrthoDB" id="508204at2759"/>
    <dbReference type="BRENDA" id="2.1.1.107">
        <property type="organism ID" value="2687"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902326">
        <property type="term" value="P:positive regulation of chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090352">
        <property type="term" value="P:regulation of nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900058">
        <property type="term" value="P:regulation of sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="121"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ84887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="383" mass="40022" checksum="47DD11CBE7D247A6" modified="2011-05-31" version="1">MALALRAPRFQPLPASIPATSTAFASTARHSSSAAAICAAASPFTEATSSSRYRRDAWSYTAQDSSSPSSAAAAAAAAASGRRDDEIALQLPELRRLLKALRASRGKGLEGKDGGGGPGRVALVGTGPGDPELLTLKAVRAIEAADLVLYDRLVSNEVLDLVAEGARLLYVGKTAGYHSRTQEEIHELLLSFAEAGANVVRLKGGDPLVFGRGGEEMDFLQQQGIKVEVIPGITSASGIAAELGIPLTHRGVATSVRFLTGHSRNGGTDPLHVAENAADPDTTLVVYMGLSTLPSLAPKLMKHGLPADTPAVAVERGTTPQQRMVFAMLKDLVNEVHSADLVSPTLIIIGKVVALSPLWVESSKQDALNNENSSQLSPTIFPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSG2</accession>
    <name>F2CSG2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Cellulose synthase</fullName>
            <ecNumber evidence="23">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC123398002</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 23">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 23">Glycan metabolism; plant cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 23">Cell membrane</location>
            <topology evidence="2 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="23"/>
    <dbReference type="EMBL" id="AK354564">
        <property type="protein sequence ID" value="BAJ85783.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948447.1">
        <property type="nucleotide sequence ID" value="XM_045092512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CSG2"/>
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    <dbReference type="PaxDb" id="4513-MLOC_68431.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0482670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0482670.1"/>
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    <dbReference type="GeneID" id="123398002"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0482670.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0482670"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQXZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001418_0_2_1"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="UniPathway" id="UPA00695"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CSG2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010330">
        <property type="term" value="C:cellulose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:cellulose synthase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="term" value="P:rhamnogalacturonan I side chain metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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        <property type="entry name" value="mRING-HC-C4C4_CesA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027934">
        <property type="entry name" value="CES_Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14569">
        <property type="entry name" value="zf-UDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 23" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 23" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 23" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 23" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 22 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="832"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="864"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="899"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="953"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="985"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1016"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="BAJ85783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0482670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1055" mass="118595" checksum="1C4F32113C4A8FD1" modified="2011-05-31" version="1">MEAGAGLVAGSHNRNELVLIRGHEDHKPVRALSGQVCEICGDEVGRTVDGDLFVACNECGFPVCRPCYEYERREGTQNCPQCKTRYKRLKGSPRVEGDEDEEDIDDLEHEFNIDDDKHQQHAALHSTHITDAMLHGKMSYGRASEDGGDGNNTPMVTVGIPPIITGNRSMPVSGEFPMSAGHGHGDFSSSLHKRIHPYPMSEPGSAKWGDEKKEVSWKERMDDWKSKQGIYGAADPDDMDADVPLNDEARQPLSRKVSIASSKVNPYRMVIILRLFVLCVFLRYRILNPVPEAIPLWLTSIVCEIWFAVSWILDQFPKWYPIDRETYLDRLSLRYEREGEPSMLSPVDLFVSTVDPLKEPPLVTANTVLSILAVDYPVDKVSCYVSDDGASMLSFESLSETAEFARKWVPFCKKFNIEPRAPEFYFSRKVDYLKDKVQPTFVQERRAMKREYEEFKVRINALVSKAQKVPDEGWIMKDGTPWPGNNTRDHPGMIQVFLGHSGGLDTEGNELPRLVYVSREKRPGFQHHKKAGAMNALIRVSAVLTNAPFMLNLDCDHYINNSKAIRESMCFLMDPQVGRKVCYVQFPQRFDGIDAHDRYANRNTVFFDINMKGLDGIQGPVYVGTGCVFRRQALYGYNPPSGPKRPKMVTCDCCPCFGRKKRKGGKDGLPEGVADGGMDGDKEQMMSQMNFEKRFGQSAAFVTSTFMEEGGVPPSSSPAALLKEAIHVISCGYEDKTDWGLELGWIYGSITEDILTGFKMHCRGWRSIYCMPKLAAFKGSAPINLSDRLNQVLRWALGSVEIFFSRHSPLLYGYKGGNLKWLERFAYINTTIYPFTSLPLLAYCTLPAVCLLTGKFIMPPISTFASLFFISLFISIFATGILELRWSGVSIEEWWRNEQFWVIGGVSAHLFAVIQGLLKVLAGIDTNFTVTSKATGDEDDEFAELYAFKWTTLLIPPTTLLVINIIGVVAGISDAINNGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVIIWSVLLASIFSLLWVRIDPFTVKAKGPDVKQCGINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTY7</accession>
    <name>F2CTY7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123447828</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
            <tissue evidence="3">Seed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ86308.1"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK07142.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343950.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
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                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        </subcellularLocation>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="AlphaFoldDB" id="F2CWD7"/>
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            <end position="196"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
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    <sequence length="265" mass="28880" checksum="BABAE46774FF99F9" modified="2011-05-31" version="1">MATISSCSRLCGGGSTAFHRRRSRPARATVVTCRRFSAVVRAAASATAAAPASTVPQTKECKLPTWAEFELGKSPVYWKTTNGLPPAPGEGLKIFYNPGTTKLTPNEQFGIAFNGGFNQPIMCGGEPRQMTLQERGKACPPIYTIRIRVPQHAMTLVFSFTNGSEWDGAYTLKFKVPKPWLNKPLSFFNEGLADELNMEGACDRAIFPDENIVITSCDIGGFYEEGGDRCKLDIVTGCMDRTSHMFDPLATIDDGSCPLDSDSEE</sequence>
</entry>
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    <accession>F2CXE8</accession>
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        <recommendedName>
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    <organism evidence="10">
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
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                <person name="Kamiya K."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Shoot</tissue>
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    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">cv. Morex</strain>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
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    </reference>
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        <subcellularLocation>
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            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
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        <property type="molecule type" value="mRNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF48">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019581" description="Transmembrane 9 superfamily member" evidence="9">
        <location>
            <begin position="34"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="557"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ87519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0455440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="595" mass="67668" checksum="9AE4A3E2B3110BFF" modified="2011-05-31" version="1">MLPSPRGSGRRSLVLVLSLTAVLAFASPHRASASESDHKYKSGDSVKLWVNKVGPYNNPQETYNYHSLPFCQPSENPGHKWGGLGEVLGGNELIDSQLDIKFLKNVERGSICTLELDSKKIQQFADAIESSYWFEFFIDDLPLWGFVGETDKNSENKHYLYTHKNILVKYNDNRIIHVNLTQESPKLLEAGKKLELTYSVKWVPTDVSFARRFEVYLDYPFFEHQIHWFSIFNSFMMVIFLTGLVSMILMRTLRNDYAKYARDDDDLESLERDVNEESGWKLVHGDVFRPPRSLTLLSALVGIGTQLAALILLVIVLAIVGMLYVGRGAIITTFIVCYALTSFISGYVSAGLYSRNGGKNWIKAMILTASLFPFLHFAIGFALNTIAIFYGSLAAIPFGTMVVMFVLWAFISFPLVLLGTVVGRNWSGAPNNPCRVKTIPRPIPERKWYLTPSVISLMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFVILLIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTALYVYLYSIYYYHVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAIGYVGSTLFVRRIYRNIKCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2CZR7</accession>
    <name>F2CZR7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10">Putative roothairless 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123448855</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Chmielewska B."/>
                <person name="Szarejko I."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <dbReference type="EMBL" id="JF421241">
        <property type="protein sequence ID" value="AEM45792.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK357123">
        <property type="protein sequence ID" value="BAJ88338.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK363753">
        <property type="protein sequence ID" value="BAJ94956.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981818.1">
        <property type="nucleotide sequence ID" value="XM_045125883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CZR7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383400"/>
    </dbReference>
    <dbReference type="GeneID" id="123448855"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0318840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0318840.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0318840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0383400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448855"/>
    <dbReference type="HOGENOM" id="CLU_420019_0_0_1"/>
    <dbReference type="OrthoDB" id="2014623at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052:SF3">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011206054" evidence="8">
        <location>
            <begin position="25"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="653"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="9">
        <location>
            <begin position="435"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEM45792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ88338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ94956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="673" mass="72202" checksum="274354B989DB9936" modified="2011-05-31" version="1">MVGSIAAQAVVIGAILLLAGLAAAQQTPRAPAAAVAPAPDPGCNGIQLTYILQSREKIRPFVSDRNKQPYSFKANASVLNGGTRPLRSWAMLVTFGHDEILVGVGGAVLTGGAELPYNTTEAAGNATSFSGYPQTDLLTPIATAGDMTQIQASVGLVGTLFAGPLGLVPDPLPTALSLDDPDYKCPAATNVTATMFTTCCLLTPEAEANVTVIDANSTDPTKSFLPRGTGDLVITYDVVQAYPTSYLALVTLENNAKLGRLDNWRLSWEWRRGEFIYSMKGAHPLAVDVSNCIYGAPGQYYQSLDFSQVLNCQKKPVILDLPLSRYNDTQMGKIEHCCRNGTILPKSMDAAQSKSAFQMQVFKMPPDTNRTMLFPPANFKISGGSSLNPDYACGQPVPVSPTGFPDPSGLDSTTLAVATWQVVCNITTAKGAKPKCCVTFSAHYNDSVIPCNTCACGCPSNRRGPTCSTTAPSMLLPPEALLVPFDNRTQKAQAWAQLKHYNVPRPMPCGDFCGVSINWHVSSDFNKGWSARVTLFNWGDVDMANWFAAMVMDKAYDGFEKAYSFNATAEGNNTIFMQGLEGLNYLVKQTNMSGSDYLVPGKQQSVLSFTKKPTPDIDVVAGDGFPTKVFFNGDECAMPQRFPIKSDGFRTHLSSALALVLLMAASALLLLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2P6</accession>
    <name>F2D2P6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395687</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK358153">
        <property type="protein sequence ID" value="BAJ89367.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946655.1">
        <property type="nucleotide sequence ID" value="XM_045090720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2P6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426640"/>
    </dbReference>
    <dbReference type="GeneID" id="123395687"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0354460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0354460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0354460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0426640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395687"/>
    <dbReference type="OrthoDB" id="591017at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046527">
        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120:SF49">
        <property type="entry name" value="PIR2-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20235">
        <property type="entry name" value="PIR2-like_helical"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIR2-like helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PIR2-like helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3615" evidence="1">
        <location>
            <begin position="493"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ89367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="696" mass="77675" checksum="91364C98918C5F52" modified="2011-05-31" version="1">MASLNAKASTGHLRVYGPALYDYGAPLMKERDQEASLLLLKIQHHYEEALRRLTVRWRSDVCLGVCVGLLDPVSNIIASTLVAATEAPVDADPTAVPPPSSPPQKLRDVERRSLDGLVAFLTCFFPYLADWEAVRYLLLAEADPIVAARIVVESRGVRCFREGSAAACGALKLALNCAMVAAKHPCPSQLAGVWLSLSSSLHNVAPLLSDVQPNFPRDILHSFHTLVKEKAHSPYPTPDGSLVRPWKLAARCRKRATKLTYRHSWSLRRVLLDTIHGFYLQALARLPCGYHRSLLDAGHCYGPFDPVSNIIINTIWYQATFPPLSQQDELDIVGTLCLMRIEARSFYGLVSFLCTCHQDLNADQAIRFLLDTALNLSATKQCSSVKEEHEAYRAATIAAWHPRPDAQAEFLSSLKAMSVAPHVLSLLQDGGQYQLSSQCVHQIHRLLCSEYHSIVGVPTQQRRPAPISERQFHFTMSEGRKQRRAHRRISAKVKAALRRYEKKDSGHSYQLHVVCGVNECVSGPDNCEGSGLKPGLDPNDDYYHHTHANFLATRNVAGIVSAPVLFFVELSNHDDDQDSQLLCCPVDLPPPGAAGLVRCLFCEHQGIRIVHPALEGFHGHEAEFEKMVRGEDLYNNDYYPEGDIEPYTNHQILEYSEDVAKWVHEGLDEDCMYLSSNDFTIEKDDSDESEEVDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2D3A2</accession>
    <name>F2D3A2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123398965</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AK358359">
        <property type="protein sequence ID" value="BAJ89573.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949337.1">
        <property type="nucleotide sequence ID" value="XM_045093402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D3A2"/>
    <dbReference type="SMR" id="F2D3A2"/>
    <dbReference type="STRING" id="112509.F2D3A2"/>
    <dbReference type="PaxDb" id="4513-MLOC_17532.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0488010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0488010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0488010"/>
    </dbReference>
    <dbReference type="GeneID" id="123398965"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0404340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0404340.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0404340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0488010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0488010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0488010"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398965"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FEICPAL"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D3A2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000285">
        <property type="entry name" value="RING-H2 finger protein ATL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF19">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="155"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="210"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAJ89573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0488010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="358" mass="37897" checksum="4999F34AD40167D2" modified="2011-05-31" version="1">MSPHSSFWSPPPSVVTLNCTDNSLECHRSCPGVDCPDYAAPPPPPLPLAPAAVDHRLPVRLLLTVSLLSAFLFLSLGLATLLLYRRRRALRRRRRAATAPLPHGDGFGDGGDEEAGGGGGVVHHVWYIRTVGLDEATIASIATKEYRGVGTGGDCAVCLGEFSDGELVRLLPRCSHPFHAPCIDTWLRAHVSCPICRSVVVVPSGLPAAATDAETEGGQVEERQVFDEMSPSESLPEGSEDSDASSDTQSDIQSEDTEVVAEENGSATPKPIRRSASMDSPLFLVVVPEVQGGALQANRKLPSGRPMSIFRAKEKEAAGTSSSSCQAGGFRIGRSMSSSGRGLFFARNGRSTGNVLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2D9N8</accession>
    <name>F2D9N8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVA22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123401581</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK360600">
        <property type="protein sequence ID" value="BAJ91809.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK368159">
        <property type="protein sequence ID" value="BAJ99362.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951345.1">
        <property type="nucleotide sequence ID" value="XM_045095410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D9N8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549340"/>
    </dbReference>
    <dbReference type="GeneID" id="123401581"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0456480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0456480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0456480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0549340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401581"/>
    <dbReference type="eggNOG" id="KOG1726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028431_5_0_1"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D9N8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ91809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ99362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="289" mass="32448" checksum="4E8FF15953D05E47" modified="2011-05-31" version="1">MVVGSFITRALILVLGYAYPAYDCYKTVELNRPEIEQLRFWCQYWILLAMLTVFERIGDNFISWLPMYSEAKLAFIVYLWYPKTQGTSYVYESFFKPYIAKHESEIDRNLLELRTRASDMAVIYFHKVADYSQSRFHEILQYIASQSQGSRPQAQQQQQRPPPPRTRMANPAPPPVPAPTAPPMPPQPAQVPPTPPRMQVQDKGPVPVAPPGAVPPVQQQTPSGPGPVAGNSPPNSEDMLVDQAGPSTSNMPPPRPTMPEDEETLIHEAIRLTRGRLRRRMGGSGPPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2DA47</accession>
    <name>F2DA47_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123404827</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the type IV zinc-finger family. Class B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK360760">
        <property type="protein sequence ID" value="BAJ91968.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044954706.1">
        <property type="nucleotide sequence ID" value="XM_045098771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DA47"/>
    <dbReference type="SMR" id="F2DA47"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579890"/>
    </dbReference>
    <dbReference type="GeneID" id="123404827"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0480880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0480880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0480880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0579890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404827"/>
    <dbReference type="HOGENOM" id="CLU_059658_0_0_1"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DA47">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009646">
        <property type="term" value="P:response to absence of light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009735">
        <property type="term" value="P:response to cytokinin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000055">
        <property type="entry name" value="GATA transcription factor 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052138">
        <property type="entry name" value="GATA_ZnFinger_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255:SF14">
        <property type="entry name" value="PROTEIN CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ91968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="341" mass="36648" checksum="D6C2D47CE0AE76EA" modified="2011-05-31" version="1">MSTIYMSQLSTLPLMDGDQDQGHFQAFHLPKDPPILFPFMIDNPVDHQGQGCGDQHSGQHLFGESSQQFNDRMMMGGGSADVFATCSPFGPTIQSIGSDMIQRSSYNSYDFEATHASDGSTSQWASAKPPVKMRIMRKAPTNDPQGGMVRKPRRRAQAHQADESQQLQHAMGVIRVCSDCNTTKTPLWRSGPCGPKSLCNACGIRQRKVRRAMAAAAATGANGGVASASSGGVASGGVQTSEASQAVKATKKEKRAADLDRSLPFKKRCKMVDHPTVTSTKVVAVDATPKHQDHVVSEDVATVERLSKADPPAAFTHVFVRDEITDAAMLLMTLSCGLVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDV8</accession>
    <name>F2DDV8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123401106</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="7">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="AK362075">
        <property type="protein sequence ID" value="BAJ93279.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK373650">
        <property type="protein sequence ID" value="BAK04847.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950763.1">
        <property type="nucleotide sequence ID" value="XM_045094828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDV8"/>
    <dbReference type="SMR" id="F2DDV8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571770"/>
    </dbReference>
    <dbReference type="GeneID" id="123401106"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0474020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0474020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0474020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0571770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401106"/>
    <dbReference type="HOGENOM" id="CLU_097408_1_0_1"/>
    <dbReference type="OrthoDB" id="10264154at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000011">
        <property type="entry name" value="Glycine cleavage system H protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 6 7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="8">
        <location>
            <begin position="49"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="6">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ93279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="158" mass="17108" checksum="EF0FD7AA99054FD3" modified="2011-05-31" version="1">MAMAASRSLWASRAASFLRISTFPRAFSTVLKDLKYADTHEWVKVEGDSATVGITDHAQDHLGDVVYVELPEVGASVTQGTNFGAVESVKATSDINSPVSGEVIAVNDELLEKPALVNGDPYEGGWIIKVKVSNAGELNSLMDDEKYSKFCEEEDSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2DHR2</accession>
    <name>F2DHR2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395856</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363429">
        <property type="protein sequence ID" value="BAJ94633.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946824.1">
        <property type="nucleotide sequence ID" value="XM_045090889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DHR2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462870"/>
    </dbReference>
    <dbReference type="GeneID" id="123395856"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0383180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0383180.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0383180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0462870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395856"/>
    <dbReference type="HOGENOM" id="CLU_123034_0_0_1"/>
    <dbReference type="OrthoDB" id="780868at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039609">
        <property type="entry name" value="VQ_15/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179:SF64">
        <property type="entry name" value="OS11G0131100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="2">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ94633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="188" mass="20109" checksum="F3B3F8230E466094" modified="2011-05-31" version="1">MMLTAMQHMDSSAYASSPSASPWQQLLTAQRGHLLPYDYEDLASVPAAARAPAAHKQHRPARRVARRRPRPSRRLPTTYISADPAEFRRMVHQVTGADELLLPPTQQQTEGALLPALPARAAPSTAGGALLLPTLDTSAFLLGGYSARTTGAMPAPTRRDGSLPPDITSGSGSSSRCGFPTLESWDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2DPY1</accession>
    <name>F2DPY1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123430705</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK365949">
        <property type="protein sequence ID" value="BAJ97152.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK367732">
        <property type="protein sequence ID" value="BAJ98935.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970487.1">
        <property type="nucleotide sequence ID" value="XM_045114552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DPY1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190340"/>
    </dbReference>
    <dbReference type="GeneID" id="123430705"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0157690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0157690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0157690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0190340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430705"/>
    <dbReference type="HOGENOM" id="CLU_085549_2_0_1"/>
    <dbReference type="OrthoDB" id="1914452at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011206252" evidence="6">
        <location>
            <begin position="25"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ97152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="189" mass="19036" checksum="CC5B14F7D813B28F" modified="2011-05-31" version="1">MDLRALALAAAVLLALAAPVPVLGQGVATSCTASLITSFTPCLSFITNSTNGGGSSPTADCCRSLSAVVTTSTSCACLILTGNVPLGLPINRTLAVTLPKACNSMSVPLQCKDTSAQLPAPGPVAVSPAMPPLPPMTPEPPAPPSETTVTMPPTSQTQGETRPQVVPSSAWRDSAPLSVMLFIVGAMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQS9</accession>
    <name>F2DQS9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123449767</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBOH (TC 5.B.1.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK366247">
        <property type="protein sequence ID" value="BAJ97450.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983026.1">
        <property type="nucleotide sequence ID" value="XM_045127091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DQS9"/>
    <dbReference type="SMR" id="F2DQS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409670"/>
    </dbReference>
    <dbReference type="GeneID" id="123449767"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0341190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0341190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0341190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0409670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449767"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000049">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000019">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000007">
        <property type="entry name" value="Respiratory burst oxidase protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013623">
        <property type="entry name" value="NADPH_Ox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF197">
        <property type="entry name" value="RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08414">
        <property type="entry name" value="NADPH_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="241"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="17">
        <location>
            <begin position="602"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ97450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="916" mass="103235" checksum="406F6E3E87EADB9D" modified="2011-05-31" version="1">MHNRAGGGGAGEIVEAGERVVPHSGPLGGKRSAMRKSARFAESVSAPLTAPHGAPRGGGGNDDDDDYVEITLDVRDDSVAVHSVKPAAGGEDSDVKLLAQTLEKRSSSYGQGVLRNASTRIKQVSQELRRLASVNRRGGGGAGGPGRVDKSKSAAAHALKGLKFISRTDGSAGWPAVEKRFDDLAENGLLHRSKFGKCIGMKELAFAGELFDALARRRNIAGDSISKAELLEFWDQISDTSFDSRLQTFFDMVDKDADGRITEEEVKEIITLSATANNLTKVKDQSEEYARLIMEELDPNNLGYIELYNLEMLLLQAPSQSMAIGTTNSRNLSQMLSQHLRPTAEPNPLRRWYRRVSYFLEDNWRRCWVLLLWFCICVGLFTWKFMQYRERAVFKVMGYCVCVAKGGAEMLKFNMALILLPVCRNTITWFRNRTAAGRFVPFDDNINFHKVIAAGISVGAGLHIISHLTCDFPRLLRATEEEYEPMKRFFGEEQPPNYWWFVKGTEGWTGLVMLVLMAIAFTLATPWFRRGRLSLPKPLNRLTGFNAFWYSHHLFIIVYALLIVHGHFLYLTKKWQKKSTWMYLAVPMILYACERLTRALRSSVRPVKILKVAVYPGNVLSLHFSKPQGFRYKSGQYIFVNCAAVSPFQWHPFSITSAPQDDYVSVHIRTLGDWTRELKNVFSKVCRPPTEGKSGLLRAEYDRDGAMSNPSFPKVLIDGPYGAPAQDYKQYDIVLLVGLGIGATPMISIIKDIINNMKRLEGDVESGNPGDASTSTSFRTRRAYFYWVTREQGSFEWFRGVMDEIAESDKKGVIELHNYCTSVYEDGDARSALIAMLQSLNHAKNGVDIVSGTRVKTHFARPNWRNVYKRIALNHREQRVGVFYCGAPVLTKELRELAQDFSRKTNTKFEFHKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2DSI4</accession>
    <name>F2DSI4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123425225</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Removes the phosphate from trehalose 6-phosphate to produce free trehalose. Trehalose accumulation in plant may improve abiotic stress tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="8"/>
    <dbReference type="EMBL" id="AK366852">
        <property type="protein sequence ID" value="BAJ98055.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964833.1">
        <property type="nucleotide sequence ID" value="XM_045108898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DSI4"/>
    <dbReference type="SMR" id="F2DSI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123350"/>
    </dbReference>
    <dbReference type="GeneID" id="123425225"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0101600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0101600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0101600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0123350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0123350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0123350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425225"/>
    <dbReference type="HOGENOM" id="CLU_037265_1_2_1"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000099">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000175">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF56">
        <property type="entry name" value="TREHALOSE-PHOSPHATE PHOSPHATASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ98055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0123350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="358" mass="39662" checksum="94EF69BD2DC92507" modified="2011-05-31" version="1">MTNHAAFAAEDAVTAVAAPAQPGRHFASFPPRRARDCRKAALGRMDLSTSGVLMAGSLLETMKASSPRHAMSAVGADHEEWIENHPSALEWFEGVLAAAEGKQIVMFLDYDGTLSPIVKDPDSAVMTEEMRDAVRGVAQHFPTAIVSGRCRDKVFNFVKLEELYYAGSHGMDIKGPTKVSNHKAKADEILCQPATEFLPVIQEVYKTLTAKMKSIHGAMVENNKFCLSVHFRNVEEDKWDTLAREVRAVLDGYKELCLTKGRKVLEIRPSIKWDKGNALEFLLESLGYEGRSDVFPIYIGDDRTDEDAFKVLRNIGQGIGILVTKSPKETSASYSLREPAEVKEFLRKLVKSNGTKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTG1</accession>
    <name>F2DTG1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123426290</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK367179">
        <property type="protein sequence ID" value="BAJ98382.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966029.1">
        <property type="nucleotide sequence ID" value="XM_045110094.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966030.1">
        <property type="nucleotide sequence ID" value="XM_045110095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTG1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0153860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0153860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0153860"/>
    </dbReference>
    <dbReference type="GeneID" id="123426290"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0126690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0126690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0126690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0153860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0153860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0153860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426290"/>
    <dbReference type="OrthoDB" id="4771285at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DTG1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Prp31 C-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="78"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ98382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0153860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="108" mass="11114" checksum="93C2610AB7DBA5D6" modified="2011-05-31" version="1">MQFGIPEESPLGDGLGEGYGMLGQAGNGRLRVSAAQNKLAAKVAKKFKEKSYGSSGATSGLTSSLAFTPVQGIELSNPQAHGNLLGSGTQNTYFSENGTFSRMSRPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWV7</accession>
    <name>F2DWV7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123429499</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368375">
        <property type="protein sequence ID" value="BAJ99578.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969459.1">
        <property type="nucleotide sequence ID" value="XM_045113524.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969460.1">
        <property type="nucleotide sequence ID" value="XM_045113525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWV7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114880"/>
    </dbReference>
    <dbReference type="GeneID" id="123429499"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0094380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0094380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0094380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0114880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429499"/>
    <dbReference type="OrthoDB" id="677765at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ99578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="507" mass="58584" checksum="FFADC9881E339D8E" modified="2011-05-31" version="1">MGIRSRRRRPAARSVAEPAEPMEKDNQDDRISSLPNDILVNILDRLKARAAVKTKVLSRRWSQLPDMLPRLTISARDLRPSKTKTSMSNNELFQRSNAAVVKATESVLAHRDLGGCTIDLLSTTFYLKDDAPISIGRTVGNAMATHKIEKVEFTVLTEKERQECTFDDMEKYGTRFVSFFNECLNAFAGLTRLCLQNLTFRESVIVSNILVTCKQLKYLDFHYCRTEGLILDVEHTQLSELSIVNCRFGLINLTMVPKLTRLVFEYWTAYKEPPLSFGYVPLLEVLSLATVHLSWHKMVKLSTFLYGSSVRDLRLCFRFEKIWVQPECLTKRLASVLHQLRIVNLVDIPEGYDLTWTMFILEAAPSVEELYMKVMDHPCEMHMDKEKRRANLFSENKGVEWKPPASHFKHHRLAKLIIFCFESLDYMVNHVRCVMKAAVNLQDVYLYNRLACNKCRNVMPPRLIKFPETVMGRHLEGKRLTWGIESPATIHFQITPVIRAEHRARTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DYW3</accession>
    <name>F2DYW3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123445038</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369083">
        <property type="protein sequence ID" value="BAK00285.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977889.1">
        <property type="nucleotide sequence ID" value="XM_045121954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DYW3"/>
    <dbReference type="SMR" id="F2DYW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305340"/>
    </dbReference>
    <dbReference type="GeneID" id="123445038"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0254920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0254920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0254920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0305340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0305340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0305340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445038"/>
    <dbReference type="eggNOG" id="ENOG502QVKF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="SLEMPGM"/>
    <dbReference type="OrthoDB" id="2019494at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DYW3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000021">
        <property type="entry name" value="Transcription factor bHLH130 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF347">
        <property type="entry name" value="OS01G0900800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="306"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK00285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0305340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="378" mass="39877" checksum="B4CE7A7F7BFE3254" modified="2011-05-31" version="1">MNRPTSAHHGGMPPPPQLARYGSAPGSFLAALADSVSRGGGEAPASHSHSHSQQQHHHQPVAAVVSRFFSGESSGLTSCESSCRTADAPAALQRAYGGSGEIHVPPPALPQQQQQPGLLRHSSSPAGLLSRLMADPHGNGGGMGGARGGGMGGGYAHSHSHSQGGAGGNADAMAAAQQRRLSSQWSFSRQQDMMPHIAEMGMAMPTPTPMPPADVGESIATGHGSGDLSRSFSMSSWDDTNSNIIFSAPPGGKKAKVMADGDDGMVTSFSNIDSQFGSSLDMPGMDDYLQLQQDSVACRVRAKRGCATHPRSIAERERRTRISKRLRRLQDLVPNMDKQTNTSDMLDIAVDYIKVLQDQIEKLKQDQGNCSCSADQKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFT0</accession>
    <name>F2EFT0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447013</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK375007">
        <property type="protein sequence ID" value="BAK06202.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979502.1">
        <property type="nucleotide sequence ID" value="XM_045123567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFT0"/>
    <dbReference type="STRING" id="112509.F2EFT0"/>
    <dbReference type="PaxDb" id="4513-MLOC_44084.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402090"/>
    </dbReference>
    <dbReference type="GeneID" id="123447013"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0334720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0334720.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0334720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0402090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447013"/>
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    </dbReference>
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    <dbReference type="OMA" id="MADWGSV"/>
    <dbReference type="OrthoDB" id="652307at2759"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021775">
        <property type="entry name" value="DUF3339"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33128">
        <property type="entry name" value="OS05G0103400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33128:SF46">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF 3339)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11820">
        <property type="entry name" value="DUF3339"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK06202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="69" mass="7290" checksum="4D1B7845DB3DCFA5" modified="2011-05-31" version="1">MADWGPVVIATVLFVLLTPGLLFQLPAQGRIVAFGSMHTGGLAILVHAVIYFALITIFLIAIGVHIYAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>M0UDZ4</accession>
    <name>M0UDZ4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="SMR" id="M0UDZ4"/>
    <dbReference type="STRING" id="112509.M0UDZ4"/>
    <dbReference type="PaxDb" id="4513-MLOC_10090.12"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0331870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0331870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0331870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0276470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0276470.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0276470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0331870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0331870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0331870"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0UDZ4"/>
    <dbReference type="OMA" id="HVEDNMT"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UDZ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="312"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0331870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1568" mass="175646" checksum="0D8C4DCB4F8D84B1" modified="2013-04-03" version="1">MEVAVSAAWWVVTKALGPVTDGLLEAWAASAGLGPNIDALKMQLLYAQGMLNNAPGQGRDITNPALKGLLHKLRQLAYVADDALDELDYFRIQDALEGTYHAAESVDDRGCLQGLSFNARHTARAVVGKIKSMSSCSRAASPTDDQEDIGGSQGCSCVGRCSMPKVASGAVQAAVGKHFPCCYSSPHTSELAESSNMTAAGGWRLLCGACPPKAPAQRVRAADETPKLKFDRVEMSKRMIDIVEQLKHVCAMVSTILNLELLSLSRIPIKVSPAMDRPKTTPQIIEPQLYGRDTQKKEIIDGITHGVYSSKRLTVLPIVGPGGVGKTTFTQHIFEEVKSCFQVTIWICVSLNFTADRLAQEILEQIPKDNSSESKNGSSIEEKIEQRLRSKRFLLVLDDVWKYPEDEWKKLLAPFRKVGTEGNMVIVTTRIPGVANMVRTVDHSIEMERLGPQDIMSFFEACVFDDQQQPWENHPELQDVGHKIVENLKGFPLAAKTVGRLLRNQLTFDHWTSVLESKEWESEGSDNDIMPALKLSYDYLPFHLQQCLSYCALFPEDYEFHAEELVHLWIGLGILHSDGHKKRIEDVGLCYLNDLVNQGFFKKNVSTSGSPYYTIHDLLLELVVKVSSYECLSISSSNVMSIQIPPSVRHLSIIVDDADVKDTVTFEDYKRGLRALENRFEVQNLHTLMLFGEYHANFSRVFRGLFLEARALRTVFFSGASYNVEDVLPNFSKLVHLRYLRIKSAGFYDDEGLCVPSVLCRLYHLVVIDLQEWKDCFGSTRHISNLVKLRHFVVPREEPHSGIFEVGKLKFLNELKAFRVGQERKAFELSQLGKLRNIGGLLGIYNLEKVLSEEEATESKLMHKSHLQELMLEWDVERSNRVPAFEQSVLENFVPHRNMRHLCIRGHGGTNCPTWLGENHSIKNLESLSLDDLSWGNLPPLGEMWMVNDLGEEYQVCIAGQGFKILKRLRLVKIPRLRKWVADNNTVDLFSHLEELTIVGCSELIELPFSDLSYGRTQQEKSIAWFPRLRSLRIEDCPKLLLLPPIPWTCGPCSIELNRVGSSLEQLDYRERHGSDSTFSLYVVGKDGAFLKALVFSNLTFVKEIDMHQCRCTGKELTQVFSFMPVLTHIIIVGCQGIKWLGVAGQSSASCSSAELLHQEQIRQQKQEADKGGDGVLLLPPQLQRLYMEKFPELNLLSDSLEGDKEGSGLKYLCSLQSLDILDCPKLLHSYSSSSFPFPASASLQRLYLQRCGEFRGESICPLLAQGHLKTLCIFETPNVFSGSEPLLDRKLIPCSSAHQELDLRTDDVAGLLTTHICSLISSSLTTLYLGWNQDAERFTHEQEEALQLLTSLQELVFYSLEKLQCLPAGLNILANLKTLNLLSCQVIKSLPEDGLPSSLQELNIHDCPAWESLPKDGLPSSLSKLKISECRAIKSLPKGSLPSSLQELNIYNCTALMSLPKDSLPSSLQKLELHDCPALMSMPRGSLPSSLQKLEFRGCPALESLLEDGLPRFLQELKIQGNTTITSLPKGNLPRSLQVLDVRYCHNEELKRQCRKLRGTIPKIITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0UR38</accession>
    <name>M0UR38_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975423.1">
        <property type="nucleotide sequence ID" value="XM_045119488.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975424.1">
        <property type="nucleotide sequence ID" value="XM_045119489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UR38"/>
    <dbReference type="STRING" id="112509.M0UR38"/>
    <dbReference type="PaxDb" id="4513-MLOC_13347.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247130"/>
    </dbReference>
    <dbReference type="GeneID" id="123443191"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0205190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0205190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0205190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0247130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443191"/>
    <dbReference type="eggNOG" id="ENOG502QVBJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0UR38"/>
    <dbReference type="OMA" id="SSLCCTE"/>
    <dbReference type="OrthoDB" id="755659at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UR38">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36723">
        <property type="entry name" value="F22C12.19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36723:SF1">
        <property type="entry name" value="F22C12.19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="594" mass="64941" checksum="4BB77CB01F45B1E5" modified="2013-04-03" version="1">MQRSLPVGIKSENNGKRDYTGTGTVPSLHKQDSKILTKKTIKLLDGPPCSKRPKLEPTQTTRGAEAKGHESLPHKIVPELVRCTASEKSGLLKQKRISDAKRIDKKNARSGVRSKYDCFTLKSGLGNHDSVFMGNGMLGAHGLKSDIRDITNHIENLTLSELLDGTYKYSSLGREKGKKVLHTKDELLVSVRKAFFMLSDMDSSLGKDGNLTPSPRLHSATTSGCDIKDQCSDKPSSLIKDPLQTEVCNVLHCPKDILSRLTLPQGHDLDSLLSAGSESSATGSSMTIHGASLPPFTWSHSQAGVYRPSVDSGKHGSSRSNSHWQWLRVGSYPTAQDYKDLPPHPVDDLLQEMDMVKSSLIDSCSRQSNLCCTESTSGSLGQTIYSRKTGSEHGLQQLHPLDHVGSSDSFQRNDSERSLLKARQASRKILCAAETLCDMRRSTEGWSPQVYSNGTINLPKSPPDKVKARKPSSPFGTAESSSGSRNSDPGRSGNHSSKKAADRKNDSACMSNPGKGPIRWPVPVEDAASPVRPPEKGLMLDMRQNHGNAARHPIHVSSQARLEKEYESQQKLRKATLASSLGSGDWNKERNRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>M0XJQ6</accession>
    <name>M0XJQ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123442659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974701.1">
        <property type="nucleotide sequence ID" value="XM_045118766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XJQ6"/>
    <dbReference type="FunCoup" id="M0XJQ6">
        <property type="interactions" value="1338"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XJQ6"/>
    <dbReference type="PaxDb" id="4513-MLOC_60672.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229510"/>
    </dbReference>
    <dbReference type="GeneID" id="123442659"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0190340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0190340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0190340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0229510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0229510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0229510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442659"/>
    <dbReference type="eggNOG" id="ENOG502RZGQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XJQ6"/>
    <dbReference type="OMA" id="ETPPSHN"/>
    <dbReference type="OrthoDB" id="2019850at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XJQ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019324">
        <property type="entry name" value="MPP6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13582">
        <property type="entry name" value="M-PHASE PHOSPHOPROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13582:SF0">
        <property type="entry name" value="M-PHASE PHOSPHOPROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10175">
        <property type="entry name" value="MPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0229510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="202" mass="21789" checksum="3A1C295331BACE80" modified="2013-04-03" version="1">MSAKKGLSSTLRNLKFMQRAAVAQKVEDKADVEVEEAAAEVEVVMTPAANGGGVGSSVQVARKCVVVMEGNPHPGAVKGRMSFQNFNPSIDKLNDEASGRPTQSASPSNSQQDSANTSRVDDVSASRFRSFNVDSSESISLNELKRKEPELEMETPPLRKLPKTTGQNVDGRGSSQSNGRGSGKSNKHEKLDFNLLRKRKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y3L2</accession>
    <name>M0Y3L2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123448096</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980800.1">
        <property type="nucleotide sequence ID" value="XM_045124865.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980801.1">
        <property type="nucleotide sequence ID" value="XM_045124866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y3L2"/>
    <dbReference type="SMR" id="M0Y3L2"/>
    <dbReference type="PaxDb" id="4513-MLOC_65398.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0353390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0353390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0353390"/>
    </dbReference>
    <dbReference type="GeneID" id="123448096"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0294810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0294810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0294810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0353390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0353390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0353390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448096"/>
    <dbReference type="eggNOG" id="KOG0565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y3L2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF20">
        <property type="entry name" value="TYPE I INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="203"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0353390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="557" mass="61145" checksum="838AA6474F46B55D" modified="2013-04-03" version="1">MDQKINTRKKSPFGSIPAMKGRNSSFRHGAEHSTVDLKESPEHSPVASPSASSSSFFKSTSLKFSNFSSPAPTSTHIESFRVFAATWNVAGKTPDMELNLNDFLPSDDHSDVYVLGFQEVVPLNAGNVLVIEDNEPASRWLALINQALNRTSSSPPASRSFSQSASPVSALHTGSSSPLDPSLFHKNGSSPREVRRAAITRGRRLKSCTCPAERAPRRRPYSKSPSSCLMMRCGSSKNRRRYAVEGDTTTSDEEDMDVVVDGGGEASSMASDATLLLQPAAANLQRRYCLVACKQMVGLFATVWARRELVAHIGHVRLSCVGRGIMGRLGNKGCISVSMSLHQTSLCFVCSHLASGEKDGDELRRNSDVVEILKNTQFRRVCKRSGRRIPERILDHDRVIWLGDLNYRIALGYADAKKLVDAGDWAALFEKDQLKTEREGGVFRGWSEGVISFAPTYKYSWNSDSYYAGEDDDGAASKKKTKRRTPAWCDRILWRGDGIVQVAYVRGESKFSDHRPVCGAFIVEIAVLDGAAKMVKLVAATASMKVGAEELLLPRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287VDE0</accession>
    <name>A0A287VDE0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TSL-kinase interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123410401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044959274.1">
        <property type="nucleotide sequence ID" value="XM_045103339.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959275.1">
        <property type="nucleotide sequence ID" value="XM_045103340.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959276.1">
        <property type="nucleotide sequence ID" value="XM_045103341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287VDE0"/>
    <dbReference type="FunCoup" id="A0A287VDE0">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287VDE0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636590"/>
    </dbReference>
    <dbReference type="GeneID" id="123410401"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0528970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0528970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0528970"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410401"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287VDE0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055315">
        <property type="entry name" value="Cramped-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677">
        <property type="entry name" value="CRAMPED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677:SF6">
        <property type="entry name" value="TSL-KINASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="44095" checksum="8D14070CB1DDD5FA" modified="2017-11-22" version="1">MKAPQQHLKPTDTEANVKLGVNKMHCLKPGKYTHISPGNVGAKCTTGDDQSFTGSKIALKGGSSMEVPFLNLTNVSAQPPNYSGKMKLQFFPIDETIQKVLQQEKHNPYLELTLAPRKKMSSIVQHLNTKWGRSSCAKGDLMLFPYSARPDSIASSKRWTLNDSCTAADVYVAVGSPSTFRLRYGWFKPNLKQQISKTSLATTLSPEKTMGDKPSDPFKFPSRFASPSVECNTEQSVVDDNQSKVAPLSWMDCISNISFGALLSQAVPSQDSKQPPLQNSSIFQQPPLQNSSIFQQQIPATCDSFDAAIASLIARQQTGNQPKVSNPSPWDADETCHAFPPPRNQNLARMSSCGPGNSSAITSTILGAIAESNTDGNQQPWLPALYRRQERGTKAPSDTTRLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WBT6</accession>
    <name>A0A8I6WBT6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044957091.1">
        <property type="nucleotide sequence ID" value="XM_045101156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WBT6"/>
    <dbReference type="SMR" id="A0A8I6WBT6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027120"/>
    </dbReference>
    <dbReference type="GeneID" id="123407912"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0021430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0021430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0021430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407912"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="CDD" id="cd07245">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46142:SF24">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="24"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="215" mass="23325" checksum="2F35ED1883B39CD7" modified="2022-05-25" version="1">MLNTTAAMVSGGGGDKGGVLPLSSLNHISIVCTSVKESLDFYMNVLGFTPIRRPGSFDFDGAWLFNYGVGVHLLQSEHRESLPAKKEINPKDNHISFQCESMVAVERRLKELDIPYIQRCVEEGGIYVDQIFFHDPDGFMIEICNCDNLPVVPLVDQTLAMAACKRIPSVKQQQLPMSAAQAPPAPAAVATQCIPAPNPAMQRVGGEEVAHISCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDE4</accession>
    <name>A0A8I6WDE4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-gulonolactone oxidase</fullName>
            <ecNumber evidence="3">1.1.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123412546</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32363"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17587"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="EC" id="1.1.3.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; L-ascorbate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044961433.1">
        <property type="nucleotide sequence ID" value="XM_045105498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDE4"/>
    <dbReference type="SMR" id="A0A8I6WDE4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062980"/>
    </dbReference>
    <dbReference type="GeneID" id="123412546"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0050040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0050040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0050040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0062980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0062980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0062980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412546"/>
    <dbReference type="OMA" id="KNHNFAF"/>
    <dbReference type="OrthoDB" id="610608at2759"/>
    <dbReference type="UniPathway" id="UPA00132"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003885">
        <property type="term" value="F:D-arabinono-1,4-lactone oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050105">
        <property type="term" value="F:L-gulonolactone oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000033">
        <property type="entry name" value="L-gulonolactone oxidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007173">
        <property type="entry name" value="ALO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055154">
        <property type="entry name" value="GULLO2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010030">
        <property type="entry name" value="GULO_Plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01677">
        <property type="entry name" value="pln_FAD_oxido"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF131">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04030">
        <property type="entry name" value="ALO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22906">
        <property type="entry name" value="GULLO2-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0060">Ascorbate biosynthesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035172402" description="L-gulonolactone oxidase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="59"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062980.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="588" mass="63408" checksum="3AD0BDB117D8DE21" modified="2022-05-25" version="1">MSQGEQAQRRAMESLAAVLLVGILLQLAGCSPPPDPVTCTHGTSNCTVTNTYGSFTDRTICHAANVVYPRTEQELVAAVAAAAAAKRKMKVATKHSHSGPKLACPGGSEGTIISTTRLNRTVSIDAERQLITVESGMVLRDLIQTAAAAGLSLPHSPYWYGLTIGGLLATGAHGSGLWGKGGAVHEYVVGLRIVTPAPACHGFAMVRELGADHPDLDAAKVSLGVLGVVSQVTLALQPLFKRSVTFVKRNESDFTAQVTEWGRLHEFADVIWQPRNGTVTYRQDDRVDVSTPGDGLNDLFLFRPAPTALLVSGRATEEQLQENGTDTARCAAAQEGSTSRLQAFGFTNDGVVFTGYPVVGYQHRIQASGSCIDGPEDDLGSSCAWDPRIRGLFMYNSGFSVALSKAPAFVADMQQLRDLNPDAFCSAIDNRVGVVLRYVRASSAYLGKPEDCVDVDILFYRSHTDGMPRAHADVVDEIEQLALGKYGGLPHWGKNRNFAFDGAIARYPKAHKFLKVKNRYDPDGLFSSEWTDQVLGINGTPNIIKDRCAIEGLCVCSDDSHCAPEQGYFCRPGKVYKNARVCSSEEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGW5</accession>
    <name>A0A8I6WGW5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123440967</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973443.1">
        <property type="nucleotide sequence ID" value="XM_045117508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061660"/>
    </dbReference>
    <dbReference type="GeneID" id="123440967"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0048980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0048980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0048980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0061660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440967"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000318">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF159">
        <property type="entry name" value="OS05G0355300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="256" mass="28854" checksum="A89082BA407EF847" modified="2022-05-25" version="1">MERGEERRPRSQQRVAITAIELGARNDEPMQQQDGLSSSSSSSSSSYSMMQCRICHEEEDESSAAMESPCACSGSLKYAHRGCVQRWCDEKGSTLCEICLQGFEPGYTVPPKKAPVVETLVTVSEDEDDDEEEEELHGQLGMHYAASDTPMDGPDPAACSWCRPLAITFTIILLVWHLIAVVTIEAAEHCAFSLLTMYLLRATGILLPLYAVMRVVRMIQHGRRQYQLQQLLEEQRRRNASRRSQEEQQQLVISIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WKV9</accession>
    <name>A0A8I6WKV9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKV9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="111" mass="12266" checksum="ECAF1D60BF826E42" modified="2022-05-25" version="1">MNTTGSHQGPKKRIRKEAFRHRPWVRPAPEKSLHESGGGRCRSRSRRNAMATATTARRGSPSTTKHAGKVKRNTHHPAGSSAPPSAWAHVPPPHPCPATAGDNYYRGRRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WKZ3</accession>
    <name>A0A8I6WKZ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKZ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0094200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0094200.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0094200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0114610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0114610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0114610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044286">
        <property type="entry name" value="SINL_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632:SF18">
        <property type="entry name" value="OS01G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0114610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="131" mass="13958" checksum="A2EE920C4EE9A079" modified="2022-05-25" version="1">MPVHRVQYGKVVVLQVPATLEVRGLLLGDEDGRTFLIVNGALGAGTAVSVVCVRAEALVWPRYTLKVWASRPAPAPNRKGKADTIMAEIEVTSSTAPGAVAVEELAYLAVPPKLLVGVGAFRLMSLRIRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMU9</accession>
    <name>A0A8I6WMU9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMU9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0016130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0016130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0016130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26319" checksum="EE9B488DC5398DE5" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRAHVWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLPGPLLPPVRGPRPHLCPYGSQGSQARVFPDPQVELPGHGGNLYEVFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WPA8</accession>
    <name>A0A8I6WPA8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WPA8"/>
    <dbReference type="SMR" id="A0A8I6WPA8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193470.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233030"/>
    </dbReference>
    <dbReference type="OMA" id="CHRLACV"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000457">
        <property type="entry name" value="RING-H2 finger protein ATL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </evidence>
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</entry>
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    </evidence>
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        <source>
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    <sequence length="313" mass="35424" checksum="E3E241840C7FE240" modified="2022-05-25" version="1">MGGQRAEDGRPHSNLQRFLDCTTPAVDTHILPKTNGRLSNDAWHHADADSVEYFNLADLWEQYYEWSAYGAGATVQLQGGDKVVQYYVPYLSGIQLYTNKVLNASRSFGEDYAMDFWSDDDDNEKMSRSWSSTSDDSFNCDIVGGNRRRPGHLYFEFFEVCSPYGRIPLMDKVYELSQGFPGLTSLKSVDLSPVSWMSVAWYPIYHIPYQRNVKDLSACFLTYHTISSSFQDHSPETMTYGSAASGKQNDQKANTVSLPPFGLAANKIQGSLWTNPRTGDHKRIVSLFGAADSWLKQLGVRHHDFNYFVTHPM</sequence>
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</entry>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
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        </source>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218950.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="130" mass="14110" checksum="B873CC73A51B35CC" modified="2022-05-25" version="1">MAAGGASLLAFAIFSMLVMSSLGDPRPLCSNCETLCRTKGNAVVESTCGNFCDYNLSPQANCRSELLERCTADGTCCSSNGTCTCDCNNVVRDRCYEINCEGCKRDMFNMSYRACNDDCKNKCKKKGCHA</sequence>
</entry>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
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            <end position="137"/>
        </location>
    </feature>
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            <end position="395"/>
        </location>
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            <end position="467"/>
        </location>
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            <end position="599"/>
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            <begin position="1"/>
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        <source>
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    </evidence>
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    </evidence>
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</entry>
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        </recommendedName>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="145" mass="15722" checksum="7D9C3DB36BC26558" modified="2022-05-25" version="1">MSIRVLTTDFIRSCLPNHPTRKKDHTWKKPDPGTIKINVDASFCAEIRTGACGAVAKDYVGNFIAAATWVLSHVSSADSAEISAIHRGVLLASNIGCTNVTLESDSMNALEAMESPEVYMGPDVTTIIEVTILSLVFKDFLHTLQ</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="10">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="8 9">
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        <lineage>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045115703.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0054460.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
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    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
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        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF520">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="349"/>
            <end position="711"/>
        </location>
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    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="719"/>
            <end position="914"/>
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            <begin position="1"/>
            <end position="48"/>
        </location>
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            <begin position="1042"/>
            <end position="1412"/>
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            <end position="1523"/>
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            <begin position="1"/>
            <end position="17"/>
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            <end position="1098"/>
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            <begin position="1101"/>
            <end position="1110"/>
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            <end position="1136"/>
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            <end position="1232"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00022806"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF13086"/>
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            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0054460.1"/>
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    <evidence type="ECO:0008006" key="10">
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
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    <sequence length="1523" mass="170317" checksum="25808D70B7787FE9" modified="2022-05-25" version="1">MRRPNDGGDPGGRSREDDPAESSRAFDDGGDEDPQQPTPTRASSGDSLWQWRSQGLSEVVLSWSVDQILNKDLLRDKVSKIPETFNSMEQYMTSFFGPLLEEVRDDMSSSMDDISGAPYAKMLSVNAMKKGKGLYEIKLDRWMGVSGSGTDGYRPKAADVLLISETRPANKSHILKQSKSCVIVWINKVQGNKMTVKASRWMETGADGDERQQMGVNKYEKSYTEDLDKSWEILDQEAMALKSRNSSLNEEIRKEPPKGRNTLEKCSDLKELNETGMSGNSSRRWSFYAMHLTNMVTYDRVWVVLRRGLTMDTKVIMNMLGKNNSAIRHCNYCSNKSHEEIKDDLCNFKLNVSQLDAIASCISASNCCHRSSVGLVWGPPGTGKTTTVAVTLHMLLMKKQRILACAPTNMAVLQVASRLIGLIEDLSLSHHYSFGDIILYGNKDRLHVGKELSKIYLDDRVKKLLRCFNREVGWKHCVDSVLKFLKHCTSRYKLSLDIQASSDECRPTFKKYFTSNFSSLAKELVACIDTFFDHLPADTLGKNFDKMMFVKKLVHKVQQLFCADDLSDDDLFTIFKPSDELPDPSIGHYDLTDDATEDLPDHDISLDDPSEINSMCIKTLMDLSKMRFPCEENESSIRDLCLKQAKLIFCTASGSFELFRLQGVMPISILVIDEAAQLKESESLVPLLLPGIEHVLLIGDENQLSSLVKSKIAKDADFGRSLYERLCTMGYTKHLLEVQYRMHPCINKFPNANFYDNRIMDGPSVKQKDYTKNYLSGSIYGAYSFIHIENDMEMLDDLGQSSKNMVEVAVAANIIERLAKECWEKRQRTSVGVISPYTAQVIAMQERIGRKFEKHEFLSVTVKSIDGFQGGEEDIILISTVRSNKDGKVGFLSDAGRINVALTRAKHCLWILGNGATLLASNSVWAELVNDSKKRGCFFDALKDKHLAETMRLAIKRNGRAIYAAGVSSWTLRARGGSTIAGNSLPIRQRQLPGSGGARSINNCYNWQPNGHDLRSNSCRPNRPIFVPSREDIHGTHFEQQHRTFYGGGNNNQSRSIPADQCWPNRHTPSYDRHGASEGFRGHGERHLGQHYHSRTDQEPLCSTSRTANGRFTPGSVRREESHNQTSIRGAGQQPSGGYCNRDFQRTAHLLRPASSQRRFGSYGNADPHQWSMNKEKQFGNHPRRAAHTYRGQAPHQGVSGRGRDVLSFHERATRGSRDEHANNSRMKEPHSWRQNSSSGAVPRDLPVPEQRGVKRDWRKAEASDSPHQDNTKIRLAVESADEPYCEAQDDSSGAASHRLAVPEQPEREGCKPESSYSPCEDNTQARPESSDQPYGKPQDASAGAAPHELPAPDQPEIKRDECEAEPPVSSRQDNTEASPESLDEAHCRSEDTCSGAATHQPPVPELGHMDIDSCEAEATVIPDINILLELPAPDQPEMKRDEREAGLPVSSHQDNTEASPRSLDEPHCRSEDTCSGASTPQPSVPELGRMDIDSREAEATVIPDINIPLESVEPDSWIDSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6Y6</accession>
    <name>A0A8I6X6Y6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123425813</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044965483.1">
        <property type="nucleotide sequence ID" value="XM_045109548.1"/>
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    <dbReference type="SMR" id="A0A8I6X6Y6"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0138550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0138550"/>
    </dbReference>
    <dbReference type="GeneID" id="123425813"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0113980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0113980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0113980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0138550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0138550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0138550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425813"/>
    <dbReference type="OrthoDB" id="688481at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000004">
        <property type="entry name" value="Receptor-like serine-threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Receptor-like serine-threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF126">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035189823" evidence="17">
        <location>
            <begin position="32"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="19">
        <location>
            <begin position="38"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="19">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="354"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0138550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="680" mass="75240" checksum="B49AD2C2E20FA6E6" modified="2022-05-25" version="1">MAKPHRRFSPYLAGVAATFLLPVLHAPLAAADDEPPPWPTCGPYPPSGNYTPNSTYQANIHLLSATLPRNASLSPAFYATGDVGDVPDIVYGQALCRGDVANASACEACVVAAFRGAQRACPLYKDVMIFYDLCQLRFSNRNFLLDNDYIVTTYTLLRSRVVATPAFDAAVGLLLNATADHAVEDSSRRFGTGEEGLGDRRNPKIYALAQCAPEKTADVCRSCLSGIIGQLPNLFRGRAGGGMFGVWCNFRYEVYPFFSGRPLVQLPQFVEGPPSSAPPVTKGGEKRGNRTGKVLAILMPTIAMILASVVVYFFCRRKTRPEEDAYRPSTSEDIQHIDSLLLDLATLRIATDDFENSKMLGKGGFGMVYKGVLPDGQEIAVKRLCQTSRQGIGELKSELVLVAKLHHKNLVRLVGVCLEEQEKILVYEYMPNRSLDTILFDSEKNKELDWGKRFKIINGIARGLQYLHEDSQLKIVHRDLKASNILLDVDYNPKISDFGLAKIFGGDQSEDVTRRIAGTYGYMAPEYAMRGQYSSKSDVFSFGVLILEIITGRRNSGSYNTEQDVDLLNLVWEHWTRGNVVELMDPSLSNHPPVDQVLKCIHVGLLCVQRKPASRPTMSSVNIMFSSHTVRLPSLSRPAFCIQEVSVSETSTAYSEAYRLTENSTVMSSNQVSITELWPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8H1</accession>
    <name>A0A8I6X8H1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8H1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="17417" checksum="CCD6F9D2635276EF" modified="2022-05-25" version="1">MISFYAVEWHLPHRVSKQFGVQQRTPPDYVETSVKLHKTNQQSNKTVINWEEHHITWVDMWNAQRQHRVENDETPDTDEAAYLRHLEWMRTEYRVIHKAAWTRSDCLDLLPSEAVDAAFNNSIRETVGAHLDYGPLHDRVVRPSFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCN4</accession>
    <name>A0A8I6XCN4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0163380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0163380.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0163380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0196840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="46834" checksum="F131BB3D4700C83A" modified="2022-05-25" version="1">MCNPCTGATKCLGRHGKSNFTPAGPGHHHRHAFSVGRNIGFGFDQSTREHVAVEIGYINGTKACMMKTSSEKQWSCIGKPPMMVSDMPPAHVDGTLYWMSEPGHERVIVALDISTREFSILPCEPSMNNDVGHTFLVELTGTLSVVTVNARAGEMDIWMKPNNDSSWIGVYKLCLDKKLHYSLKKGQVVMPMEIDDGANGERILLNTGRALGYYDSKTGTMENLYSMDQLNLPRSSLAFPVLCQESLVRIQEEAPPSRATLFARPEGRFRCCDHPGHADATPGRRRPMLLKCEGGACVDVGDVYRSCCRRLLCESCQRRCLEHSPIRIIRLDPVFPTSNYDVTMVTPESTLPFCHPSVPGPEFIYHYTTKNGDVVCHVFISLEDYVRSRRFFHEVECGYRMEGGVVKESWVRRYLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFG3</accession>
    <name>A0A8I6XFG3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="8">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123450199</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044983506.1">
        <property type="nucleotide sequence ID" value="XM_045127571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFG3"/>
    <dbReference type="SMR" id="A0A8I6XFG3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337580"/>
    </dbReference>
    <dbReference type="GeneID" id="123450199"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0281540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0281540.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0281540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0337580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450199"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000350">
        <property type="entry name" value="Mitogen-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="52"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="344" mass="37174" checksum="F4C0AD5AC4A46EC7" modified="2022-05-25" version="1">MAAARERRLPHLHLTLDAPTWAFRCPAPASVTVATLSTSAPRPDGEFRLIDFDRLSVLGRGNGGIVHKVSHRRTSALYALKIIHRGHPGADEEVDVVRRVDSPHIVRCHSVLPTASGDSALLLELMDGGSLDSLVRGGHGGFPEEALAEVAAQALSGLAYLRARRVVHRDIKPANLLVNSAGHVKIADFGIAEVVSRAGKYRAAYEGTAAYMSPERFDTERSLHGDGDEQDRVDPYAADVWGLGVTVLELLVGRYPLLPAGQKPSWAALMCAICFGELPTLLDSTASPELRGFVAACLQKDHRKRASVAELLVHPFVTGRDVAASRRALCQVIEQRSMPISTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFH6</accession>
    <name>A0A8I6XFH6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976900.1">
        <property type="nucleotide sequence ID" value="XM_045120965.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976901.1">
        <property type="nucleotide sequence ID" value="XM_045120966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFH6"/>
    <dbReference type="SMR" id="A0A8I6XFH6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285310"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285310.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285310.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285310"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285310.4">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285310.4"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285310"/>
    </dbReference>
    <dbReference type="GeneID" id="123444288"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0236730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0236730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0236730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0285310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285310"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285310.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0285310.4">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285310.4"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444288"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
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    </dbReference>
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        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF53">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="7">
        <location>
            <begin position="129"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="194"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285310.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="299" mass="33957" checksum="ED069F1F75D7885C" modified="2022-05-25" version="1">MGAVQLESVAAQHAHAKLNMDSLPQGPSLFDGNDTARVNGSNSDEYERFEKGLMEYGCAHYRRRCRIRAPCCNDIFDCRHCHNESKNSIRIDTPRRHELPRHELQQVICSLCGTEQEVRQICISCGVCMGKYFCEVCKLFDDDVSKQQYHCHGCGICRIGGRENFFHCSKCGCCYSTVLKNSHACVEGAMHHDCPICFEYLFESRNDVSVLPCGHTIHEKCLKEMKEHCQFACPLCSKSVCDMSKAWERLDAELATLSNSFDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG61</accession>
    <name>A0A8I6XG61_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG61"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0146720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0146720.1"/>
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            <end position="137"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF12776"/>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="357" mass="40930" checksum="CE2E2C40909A71FF" modified="2022-05-25" version="1">MMTARGSPRLNLLKEVVERHRASPPTGSVEKRRRGSPKEDRAQWNASLEKDLVDLLREHATPEHKGQNGRSSEAWNKIVKKFHQKNPYARFKKNKIQEKEKELKRDYRMIKEIRKQSGVSWDDQQCKILADPPLWKNIIISHPKGGKFKTKGFPLFDALGELHDGQTAEGTYNFTSIESSQYSTEAQLENLGGAGEYLRENHGETSYHGDNIGGEPSANGENLGGERVQIDEDVEEVFVQENIAVEPHQTQPSLATSPSRNGQEKEPKRRRGSNSDVAAMMEKYLEIRAKQVEDERNKPRVVDEYSIKNCIDLLKTMDITPEEEVKAFRVFKIPENREIFMSARPDTALMWLRTEME</sequence>
</entry>
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    <reference evidence="5" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="24941" checksum="AABBD2AD52A8DDBC" modified="2022-05-25" version="1">MPTSFHKVLAHGTTMLDVVYTNLSREVPFFLQQLKEKWFDDAVDHEKFLALDLEYTANQRGVAVIQLCFARHVLIFQLARSDKHCPELMDFLRSGITFASVDIRNDKLKMRHSFGIEIPAGCLVDLQTIFRLRHDRTSMAHMAVALIDDSYADMKTSFPKSQHKLWEKGPLDDINIEYAAKDAYVSNELYRKIRVVHYGQRHLEEGGHSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIU6</accession>
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            <fullName evidence="12">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="13" type="primary">LOC123442335</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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    <reference evidence="14" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May play a role in protein folding in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044974276.1">
        <property type="nucleotide sequence ID" value="XM_045118341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIU6"/>
    <dbReference type="SMR" id="A0A8I6XIU6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218100"/>
    </dbReference>
    <dbReference type="GeneID" id="123442335"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0181420.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218100"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone protein dnaJ 50"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR044632">
        <property type="entry name" value="DNAJC25-like"/>
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        <property type="entry name" value="J_dom_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44176:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035308657" description="J domain-containing protein" evidence="11">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="297" mass="34432" checksum="452B637A782AE5B6" modified="2022-05-25" version="1">MAAAAAPLLLLLVAALLLPASDAIYCDEDDCYDLLGVKQDANASEIKKAYYKLSLKHHPDKNPDPESRALFVKVANAYEILKDEETREKYDYAVAHPEEFFYNTAQYYRAYYGYKTDTRSVLVGLLLIVSAFQYINQLTSYSQAIASVKQTPAYRNRLKALEFERTGGISSKKKGNKQMDKKVEDELTNEVDLQIQGVQKPSVWNLCGVQLILLPYLIGKLLIWQICWFWRYRVKKSPYAWEDACYLTRTSLRMPAGTWQNIDEFTKKDLVMKRLWEKANMERHIAEARRGSKQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XN61</accession>
    <name>A0A8I6XN61_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="6">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442378</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044974330.1">
        <property type="nucleotide sequence ID" value="XM_045118395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XN61"/>
    <dbReference type="SMR" id="A0A8I6XN61"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219800"/>
    </dbReference>
    <dbReference type="GeneID" id="123442378"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0183050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0183050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0183050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442378"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000485">
        <property type="entry name" value="Dephospho-CoA kinase CAB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00055723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="25508" checksum="03E3EAE6DFC9E736" modified="2022-05-25" version="1">MRLVGLTGGIASGKSTVSGLFKSSGIPVVDADVVARNVVQKGTGGWKKIVKAFGNDILLENGEIDRAQLGQIVFTDPEKRKLLNRLLAPHISCGIFWEILKLWVKGSLVIVVDIPLLFETKMDRWTDPVVVVWVDPKTQIERLMSRDGCGQEQAQSRIDAQLALDWKKSEADIVIDNSRSRDDTKEQFQEVLKQVSAPLTWKERMMSRDGLLSIVVCTAAGLLLAQKNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTB4</accession>
    <name>A0A8I6XTB4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123398306</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Membrane</location>
            <topology evidence="2 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948719.1">
        <property type="nucleotide sequence ID" value="XM_045092784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTB4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509540"/>
    </dbReference>
    <dbReference type="GeneID" id="123398306"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398306"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF58">
        <property type="entry name" value="OS03G0741600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509540.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="252" mass="26331" checksum="0C8F7B4085475B34" modified="2022-05-25" version="1">MRNSASAPPAAAMYGSYPAPPASSAGYAKIPTYPPPPSSYPNPSPVPPAAPAPIQDPTAPPSSLAKAAELVTRFREQGQALIAARRPWAEVFRSPAFSKPPNVGEAVSRMRRNTAYFRANYALAVLAVVAASLFWHPGTLFALLALCAAWFFLYFARPAEGGQPLRIFGTEFDDGTVLALLSGVTVIAMLFTDVGWNVVGSAMIGVALVGAHAALRSTDDLFLTEQEAAGNGLVAAGFSAAGPILPTYVRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTF3</accession>
    <name>A0A8I6XTF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Multicopper oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123442460</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Multicopper oxidase that may play a role in the maintenance of inorganic phosphate homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974443.1">
        <property type="nucleotide sequence ID" value="XM_045118508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTF3"/>
    <dbReference type="SMR" id="A0A8I6XTF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222680.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222680.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222680"/>
    </dbReference>
    <dbReference type="GeneID" id="123442460"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0222680.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222680.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442460"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13844">
        <property type="entry name" value="CuRO_1_BOD_CotA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13868">
        <property type="entry name" value="CuRO_2_CotA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052152">
        <property type="entry name" value="LPR1/LPR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48461">
        <property type="entry name" value="MULTICOPPER OXIDASE LPR1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48461:SF1">
        <property type="entry name" value="MULTICOPPER OXIDASE LPR1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298136" description="Multicopper oxidase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="142"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="427"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222680.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="605" mass="66161" checksum="D9C3A460B0717322" modified="2022-05-25" version="1">MVKRRFLAACLLLLATAAAALAEGEGEEGKRGVRVLDAGRLEKFVDELPDMPVLRGYGVAEGGNLVAGELTVGMHDTMWKFHRDLPATRVFAYGASKETATVPGPTIVAMKGVPTRVTWTNHLPPRHILPWDRSLATARLGNGTGVPTVAHLHGGVQQSTSDGHSMAWFTSGFAATGPHFSSPAYEYPNQQLPGNLWYHDHAMGLTRVNILAGLLGAYRVVDPATEAPLGLPSGAEFDRDLVLFDRDFMRDGALFMNATGNNPDVHPQWQPEYFGAVVVANGKAWPFLRVRRRRYRFRILNASNARFFRLSLSGGLRFVHVGSDSVYLARPVPTEGFLLAPSEMADVIVDFAGAKHDAVTLRSDAPAPYPGDPGEKADTVAVMKFVVASKPEHDPSTVPATLMPRYPKPDAREAVVTRHIAMYEYTKNGTDEPTHLYLNGAAYTDPVTETPREGTSELWEVINLTDDNHPLHVHLAVFTVLKQRSLRRVDEFRDCMRGSASGGAGGRNDAVACGMQRHLAGGRRHVVPRQERGWKNVFKVRPSTVATLLVRFKPLEESSATASGKSGGFPFDVTAGPGYVYHCHVSIKQRKPLLKSQAWSSFTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTI5</accession>
    <name>A0A8I6XTI5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTI5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0185800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0185800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0185800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0223770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0223770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0223770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0223770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="446" mass="47263" checksum="4084C52C922C4BCD" modified="2022-05-25" version="1">MALIDFIELRDDDIDIIELSSDEDTVQKDQIAAQHRAALLDRQGMFFPTAEGSQGVQAVFVAAGKGSQDVQAMFVATSEGSQDVQPVFVAASEGKQEAGEPGHALEATSSSMVMEKAPPVAGSEGSEDVQAVSVAAGEGGQEAGESGHALEATAPSMVMEKTTLVAASEGSQDVQAVLVAAGEGGQEAAESGHALEATTLSLVTETPPLDTAKSPYCHPSPTSAPLPSLTSTTPKAQTFEGGDAKSRRVKRKRRKKNYHTDTPRKSPRLIQMGECCASPVEEPAADHKRSRMLGPAEEATASTDSAETVVLALPHAGSTNCLRSPTSATFPSLTSISTKVMTSEGGDAKLVSRRVKHPERNYHILSLVMEKPPLGPTSTSPKALSSEGGDTKLVSGKNWVVFWRTWKWRAAAVTWELRKLVGLLCHLGSKLRYNQTYSRLKIMNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTN4</accession>
    <name>A0A8I6XTN4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTN4"/>
    <dbReference type="SMR" id="A0A8I6XTN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0489460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0489460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0489460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RAB"/>
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    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0489460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="18218" checksum="4237832ED8ABF1E7" modified="2022-05-25" version="1">MVTIDKKKIRLQIWDTAGQEAFRSITKSYYRSAVAALLVYDITRRETFNHVASWLEEMREQADGNNKITIMLVGNKSDLGQRRAVSTEEGEQFAKENGLAFMETSARTRHNVEEAFLQSSSMVYEKIQEGAIDLSKSSGVTPGLDDEFSGLEPLPRASVCCSS</sequence>
</entry>
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    <accession>A0A8I6XTX6</accession>
    <name>A0A8I6XTX6_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTX6"/>
    <dbReference type="SMR" id="A0A8I6XTX6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0483100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0483100.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0483100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0582710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF202">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF070"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        </location>
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        <location>
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            <end position="138"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582710.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="17782" checksum="BB5F45D08686F593" modified="2022-05-25" version="1">MVSKKMPKGMSGFFSVRQKPSDKWGVEFSDVGRCWWIDTYPSAHEAVRAYDVAVWRAERPREHLNFPQIESRVEAEILVLQNIKMKEITTKKSSVVVHASETDEEAMARFPREHPEYVQAELEHYSKCEAEHKKKEDEAGPSMVIPIESSSEED</sequence>
</entry>
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    <accession>A0A8I6XU82</accession>
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            <fullName evidence="2">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045092137.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493280"/>
    </dbReference>
    <dbReference type="GeneID" id="123397601"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0408700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0408700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0408700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0493280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0493280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0493280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397601"/>
    <dbReference type="OrthoDB" id="613853at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
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        </location>
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            <end position="27"/>
        </location>
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            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0493280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="541" mass="60382" checksum="1DE3D5F02AEEE7CE" modified="2022-05-25" version="1">MSSIVRTGAGTVSPDDCTAERLVEPRPRPHLRHPAMQDPPPPGENACKQNGSPCQQDDGSQDGKVIPDLPEDIRQHIHALMPLRDAARAACASHSFLRSWRCHPNLDLSQEILGFDGDLISKVDCILKNHSGVGMKTLKLELYGYNKVDSSYVDRWIHIAVTAGVEELTIIMPQVLEGAYNFPCPLIFNRSENSIRYLYIAMCAFRPTAGLGCCRSLTRLLLSNVWIADDELTGLLSSCAALEHFELMNCREIVCLKIPFLKWLTFLRVSRCKNLQVVDSNAPNLSTFCLFGNLVLSVLFGSDVKNIEISCLKFGPPNIVYFARTQLLLGAPDVERLVITSPNEMCSTPILSSKFLQLKYLHISLIGNEAISPDYDYLSLVSFLEASPCLETFIFEVQQPDMQHDSVIGNPSHLRRLPQHSHKSLKSVTIVGFCSATSLVELACHIIEKAASLERLTLDTSHGCRSPGGRSVDKPDRWYHNTVIGVLMTAPPDRCIPMWDHGIEESLRARSAIRKHIEWKVPYGVEFEVVEPCSRCVMLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWK6</accession>
    <name>A0A8I6XWK6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWK6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0354650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0354650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0354650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0426890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0426890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0426890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF24">
        <property type="entry name" value="TTF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="34"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0426890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="108" mass="12262" checksum="AE2D13BF3C1FCCC0" modified="2022-05-25" version="1">MSSTTLKSLEHSEFKISCTKFASSFSSSRSCDVDLNDLISELTVIQSTLPDRAMSVMDIFEFVRESDCYPNISIAYRIFFTMPVTVTSAERSFSKLKLLKNYLRSTMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYJ2</accession>
    <name>A0A8I6XYJ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter G family member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">White-brown complex homolog protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123396815</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential transporter for growth and development under abiotic stress. Mediates shoot branching by promoting the outgrowth of lateral shoots. Required for salt tolerance via Na/K homeostasis, at least partly by regulating SKC1/OsHKT1;5. Necessary for hypodermal suberization of roots, which contributes to formation of the apoplastic barrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044947561.1">
        <property type="nucleotide sequence ID" value="XM_045091626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYJ2"/>
    <dbReference type="SMR" id="A0A8I6XYJ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0479100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0479100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0479100"/>
    </dbReference>
    <dbReference type="GeneID" id="123396815"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0396970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0396970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0396970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0479100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0479100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0479100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396815"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000530">
        <property type="entry name" value="ABC transporter G family member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF73">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER STR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="715"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="745"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="849"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="125"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="516"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="516"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="530"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0479100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="880" mass="98779" checksum="CDDFF5147C1E320D" modified="2022-05-25" version="1">MQQPQQLRTAMRRDGERDHRRAAAAERPAAHRTERAAADETAHRRAAEAPPRRTERAAEVPAHRTERAGETAATARRAAEMPARRTTERKKSLESLLDAADVRGKRGGGPVPVGEKITTFPGQGLEFKDLSYSVVKKQKKDGVKIKKEVYLLNDISGQALRGQVTAILGPSGAGKSTFLDAIAGRIAKGSLEGSVSIDGRPVTTSYMKQISSYVMQDDQLFPMLTVLETLTFAAEVRLPPSLSRAEKLKRVWELIEQLGLQTTAHTYIGDEGVRGVSGGERRRVSIGTDIIHKPSLLFLDEPTSGLDSTSAYSVVEKVRDIAKGGSIVLMTIHQPSFRIQMLLDRIVILARGRLIYLGNPTTLPTYLAGFGRPVPEGENSMEYLLDVIKEYDESTLGLEPLVAYQRDGSKPTEAAKTPVPRTPRTPYQKSVQFRQMQLKSNQFSLASATPHANPFSNFESYNIDDEEGDFDNSLERKTQTPLHTVASGYHPRLASQFYKDFSVWVYNGVAGTPQRRPTWTPARTPARTPMSSYQRSRVNTPHRSIPPSPQEPVFKLEEPDYEEQGLDIEPLDAPEDGPKFANPWLREVAVLSWRTGLNVVRTPELFLSREVVLTVMALILSTLFHRLSGSDFLTINRILNFYIFAVCLVFFSSNDAVPTFIQERFIFIRERSHNAYRASTYVISSLIVYLPFFAIQGFTFAVITKFMLHLNSSLLYFWIVLFASLITTNAYVMLVSALVPSYITGYAVVIATTALFFLTCGFFLKRNKIPIYWRWLHYISAIKYPFEALLVNEFKGSHCYTGTQNQLSPGPLGEIKQSSLHTQLNPNITTCPMIGQDVLTSMDITMDSIWVDVAILLAWGVLYRLFFYVVLRFYSKNERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0C9</accession>
    <name>A0A8I6Y0C9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0C9"/>
    <dbReference type="SMR" id="A0A8I6Y0C9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637680.1">
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="1"/>
            <end position="21"/>
        </location>
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            <begin position="22"/>
            <end position="204"/>
        </location>
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        <location>
            <begin position="38"/>
            <end position="132"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="22217" checksum="6595E0C54E3F5852" modified="2022-05-25" version="1">MSVTSILFSAITIMLLSTTIATESTASGGGNPKATNFMVEACKNASTKSQIYDPNPITQEFCVLSLKLDNRSAEAKDVHSLVHVAIDILKGQVATANDNVKQMMHDTKNGTSTMRSLSFCVVDYNRMVSILSICDTMINEYHGRTGRADDGLLSSELPACVEKVDKPFIDCWLGLLGMEVKKLLDENIAVGMLVKLNFYLASII</sequence>
</entry>
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    <accession>A0A8I6Y1I3</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1I3"/>
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    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="190" mass="20348" checksum="12A80BC2360F23CD" modified="2022-05-25" version="1">MWHYAGPSDSTRSHPECVTGESVRAWVRGITGACANPEGARRVKPFRADNPPPNEEWTNWYSAVSNGNPAEEKEGSQEGSADSAEYVSDSGETEEETEEEEEEDGEQSSPPSPPEHRTKRRHDPAAPPAPPATPSARPAPPVALSARSVKRTRDAAAEPTDQPSKVAKPSGPKPRKALPLMRIAVPVASA</sequence>
</entry>
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            <fullName evidence="2">Cupin type-1 domain-containing protein</fullName>
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        <name evidence="3" type="primary">LOC123446918</name>
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        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3C5"/>
    <dbReference type="SMR" id="A0A8I6Y3C5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0399610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0399610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0399610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0399610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0399610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0399610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd02245">
        <property type="entry name" value="cupin_7S_vicilin-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF7">
        <property type="entry name" value="OS03G0197300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="2">
        <location>
            <begin position="95"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="2">
        <location>
            <begin position="317"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0399610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="754" mass="84460" checksum="2A8798BECA3CAA63" modified="2022-05-25" version="1">MHGKHAHSPPRSSRHASPATASRRLHMRRHIPPFLSQTARGRGESRKQGPANIANMAAATARWLVLSLLVSALWASSAAAAHETKWKAGAAVGGQVVEKERRRVVVESEAGTVTATDVADAAGTVYRLQFITMDPGALFLPVQLHADAVFYVHSGRGKVTYIQEGGSETSSLEVQRGDVYNLEQGSILYIQSYPNATRERLRVYAIFTSNAINCDDPSHPKSEAYSSVSNLLRGFDVKILRQGFGVSSGVVEAITSAKSPQSIITYKPDQEKEESSNWTEEIFDALWGDDSPLNKKKKKDKHKKKDKKDDKSKSKTFNFYSGEPDVKNCFGWSKTMTNKDLQNLRGSDIGMFMVNLTTGSMMGPHWNPKATEIAIVTHGSGIVQVVCPSIPSGAGGDHGHHDEGGRGGGDHGHHDDGGRRRGDHGHHDEGGRRRGGHGHHDEGGNDGQEGIECKNSVFGVKEGDVFVVPRFHPMAQLSFNNGSFVFVGFSTHMGENNPQFLAGDHSVLQVIGKEIVALALGQKNTTAVEKLLSAKSGSTIMACISCAEELERKAEQEEHEGGKGEREREREEEEERERKQREEDERKKREEEERKKREEEERKKREEEERERREEKERKKREEEERERREEKERKKREEEEAARREQEEEEERRRREEEEGGGGQGGGGGDDPREEEIQRRREEEEEGGGRGDDPREEEERRREEEGGRGGRGGDDPWEEEGGDWGDSTKYRAAMATNLKKRYRGRKGSVFRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4G1</accession>
    <name>A0A8I6Y4G1_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="primary">LOC123399318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949666.1">
        <property type="nucleotide sequence ID" value="XM_045093731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4G1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0528590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0528590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0528590"/>
    </dbReference>
    <dbReference type="GeneID" id="123399318"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0439680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0439680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0439680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0528590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0528590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0528590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399318"/>
    <dbReference type="OMA" id="CAMIQEY"/>
    <dbReference type="OrthoDB" id="1045822at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0528590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="150" mass="16058" checksum="870C0612828C6C2F" modified="2022-05-25" version="1">MKPGSEAPGAPSAWSSGLFDCFDDCGLCCLTCWCPCITFGKVSEIVDRGSTSCGTGGALYSLLACFTGCHWIYSCTYRSKMRAQYALPDAPCCDCCVHYCCEPCALVQEYKELKARGYDPDIGWQLNVERGNGGAGTGGVNPPGMQEMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5F2</accession>
    <name>A0A8I6Y5F2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123402693</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952568.1">
        <property type="nucleotide sequence ID" value="XM_045096633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5F2"/>
    <dbReference type="SMR" id="A0A8I6Y5F2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623260"/>
    </dbReference>
    <dbReference type="GeneID" id="123402693"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0517230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0517230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0517230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0623260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402693"/>
    <dbReference type="OMA" id="IYFEPRC"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000023">
        <property type="entry name" value="Senescence-specific cysteine protease SAG39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035295709" evidence="9">
        <location>
            <begin position="24"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="37"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="123"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="37020" checksum="FC65816451F8E169" modified="2022-05-25" version="1">MTISKALILGILGCVCFCSSVLATRELNDDLSMVARHESWMVQYGRVYKDETEKAQRFGVFKANVMFIKSFNVKNRKFYLGVNQFADLTNEEFKTTKANKGYRPSLKRIPTGFRYENVSFDALPVTVDWRTKGAVTPIKDQGQCGCCWAFSAVAATEGIVKLKTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLTTESNYPYTAADDKCKSGTNGAATIKSYEDVPANNEGALMQAVASQPVSVAVDGGDMTFQFYKGGVMTGSCGTDLDHGIAAIGYGKTSDGTKYWLLKNSWGTTWGENGYLRMEKDIPDKKGMCGLAMEPSYPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y642</accession>
    <name>A0A8I6Y642_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y642"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="29028" checksum="045DC0ADA20D61C7" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTTMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICIVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNLEFCYVPASQSGEEGYLCGVYFDSSMEDPLLQSTVEMLENRDRVARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8A1</accession>
    <name>A0A8I6Y8A1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y8A1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0520970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0520970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0520970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035225562" evidence="2">
        <location>
            <begin position="26"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="9917" checksum="4B94B8C238F8911B" modified="2022-05-25" version="1">MASKAFPALVLLMLVCGQLVVDGHAQTYIGGGGGGIRGGGSAAGLRPPTYTATHGHSSTIGEYRGARRPSAVEEEKAFMVNSLPAHDRPLPVPPSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAY9</accession>
    <name>A0A8I6YAY9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAY9"/>
    <dbReference type="SMR" id="A0A8I6YAY9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0413050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0413050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0413050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF54">
        <property type="entry name" value="OS10G0105900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="451" mass="50222" checksum="F38B622B9763D19A" modified="2022-05-25" version="1">MVTFTARRSEPELVRPARRTPVETKALSDLDDQWSLRFYESIVGFFRSPPGETVKPGKVARGIKAAVAAALVYYYPMAGRLRKLPDGNKLVVDCTGEGVMFVEATADVRLEDLGQPLVPPYPCVEEFLGDAGDTRVVVGKPLLFLQVTQLKCGGFVIGLHMCHCIADGFGILQFIKSIADFACGELVPTTLPVWKRDTFTTRMPPSITHVYPAYKPFIQGLDCMGDDVMLSTPPESMEVQYLFFGPKEIEILRSHLPEHTSKSTTTFELLTAVMWRCRTLALGYGSSQKVRIMFTLNTRGRSINGESAVPRGYYGNAHFSPMVEVTVNGLVTQPLEHVLEMIHKVKLDTTKDCMKSMVDLMALWRERSPFGMDRTYEVSDTKWVGGNALRFGKAELVAAGTPHAGDFTSKLISYHTKCKNEDGEDSTVVSILLPKLAMVRFTKEMAIWLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCA0</accession>
    <name>A0A8I6YCA0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123411461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960342.1">
        <property type="nucleotide sequence ID" value="XM_045104407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCA0"/>
    <dbReference type="SMR" id="A0A8I6YCA0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0682080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0682080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0682080"/>
    </dbReference>
    <dbReference type="GeneID" id="123411461"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0566230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0566230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0566230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0682080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0682080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0682080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411461"/>
    <dbReference type="OrthoDB" id="430315at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000001">
        <property type="entry name" value="THAUMATIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035289449" description="Thaumatin-like protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0682080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="40301" checksum="985F2A1012281B22" modified="2022-05-25" version="1">MEAPRNHSAPCLLLLLALAAQWCSVSTASCSFTISNYCTHTIWPGTMAGAGTPQLPTTGFRLDPGQTVRIPAPAGWSGRIWARTGCNFSTDGSGAAAGAVACQTGDCGGGHMECGGTGGKPPATLFEITLGKGGPADQDFYDVSLVDGYNLPVVAVPRARQGSCNATGCAADLNLSCPKELQVAGGNGGGPVACQSACEAFTQDKYCCSGAYATPDTCSPTAYSSVFKSACPRAYSYAYDDGSSLFTCNAVDYTIAFCIPPAGLNMPADANGAPPADNNGAGSTYVPPATGNSGAGRAYMPPPTGNDGAGSAYQPPPTDNNGVGSAYQTPPTGTNGIGSTYETPLASSNGVGSAFQPPLTGYNNGVRSGYQPVMTPSAASTRHAQLWFLLPAALVFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YD23</accession>
    <name>A0A8I6YD23_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123396036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947002.1">
        <property type="nucleotide sequence ID" value="XM_045091067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YD23"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432270"/>
    </dbReference>
    <dbReference type="GeneID" id="123396036"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0359300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0359300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0359300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396036"/>
    <dbReference type="OrthoDB" id="1921318at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044522">
        <property type="entry name" value="TSO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159:SF6">
        <property type="entry name" value="OS12G0605300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159">
        <property type="entry name" value="PROTEIN TESMIN/TSO1-LIKE CXC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="95"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="225"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="414" mass="45136" checksum="6FF376360B45D0FC" modified="2022-05-25" version="1">MAAAGDPEEPPRPPPPLEPPGSEAPPDDPAPAPAPVPVPVPPPPPAPAPAPPATEAMALVSAPAPAAVWTAVSPPAEANGSSDRRKKRKAEDGEGCRTCSCKKSRCLKLYCVCFASGSHCSELCGCDPCYNKPIHGVQQNTPPGLPLQVVRTAEAGQNSAEFARYQMDFFRRKCTCKKSGCLKKYCDCYQGGAGCSINCKCDDCKNPYGKKVGVILDGKSILAAPTPGPMPIERNGTGVISSDDDDDDEDDYYMNRPLSPIPPSPVSRESSFQQETLVGVEVHTMNGHLYPKPLTQVRPESWQLSRRPVEEARGEQWRYMRRPSEDGTSEGMEGHADPKFHQRDNSNKPRESHADNRFSIPRCIEVMNGMADLSPVDKSLAPDVFLDPSNREIFLSLSVDIRTMWLKRKMRSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDS2</accession>
    <name>A0A8I6YDS2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="8">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123401669</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of 2 alpha and 2 beta subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044951449.1">
        <property type="nucleotide sequence ID" value="XM_045095514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDS2"/>
    <dbReference type="SMR" id="A0A8I6YDS2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0616110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0616110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0616110"/>
    </dbReference>
    <dbReference type="GeneID" id="123401669"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0510880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0510880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0510880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0616110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0616110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0616110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401669"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="10">
        <location>
            <begin position="64"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0616110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="388" mass="42493" checksum="43B932B932B58DC0" modified="2022-05-25" version="1">MAAAALLRRLPAARPPLMAAARAISDSTAPLTIETSMPFTAHIVDPPSRDVATTPAELVAFFHDMSLMRRMEIAADSLYKAKLIRGFCHLYDGQEAVCAGMEAAITRKDSIITAYRDHCIFLSRGGDLVTAFAELMGRQVGCSRGKGGSMHFYKKDANFYGGHGIVGAQVPLGCGLAFAQKYRKEDTVSFSLYGDGAANQGQLFEALNISALWKLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDYVPGLKVDGMDVLAVKQACKFAKEHAIANGPIVLEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERVRKLILAHDLATPAELKDMEKEIRKEVDAAIAKAKESPMPDASELFTNVYVKGFGVESFGADRKELRATLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGU5</accession>
    <name>A0A8I6YGU5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410619</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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            <strain evidence="4">subsp. vulgare</strain>
        </source>
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    </evidence>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.4HG0324030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0389490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389490.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34686">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17889" checksum="F8FAAB207AD6F715" modified="2022-05-25" version="1">MSSRPSRSDAHLSPVDEAVRVDEVRGYYDGAAPKRHSKPSRSDHSAVYTDALAGPGVDGSHPELDKFQDLEAHSEKLVCEGGKAGEEFVETEYYKDLGCVGKQHHTTGTGFIKVDRPAGGSFELSEDPGATERHASSKGNPATNDWIPSADTVYQEASDKPNRSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMT8</accession>
    <name>A0A8I6YMT8_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMT8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0523580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0523580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0523580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0523580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0523580.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0523580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0523580.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="169" mass="18993" checksum="1E2C3C61BEDE7478" modified="2022-05-25" version="1">MAAEKMQFAMPMATSKVGKEEASLKAKAKAEKESAVLKTKAETEAEEAIVKAEAEKEEAIIKAQAEAEKEEGIIEANCCCGSNEDDAPYDKLWHDSDTEEDPDIIMAKTVERWDLFIKEQEARFPHLQYGDSCPREYTVDDQEGSADDQEGSDHDEQYYHRLLDEGLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNP3</accession>
    <name>A0A8I6YNP3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone deacetylase</fullName>
            <ecNumber evidence="4">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123395110</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNP3"/>
    <dbReference type="SMR" id="A0A8I6YNP3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0437670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0437670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0437670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09992">
        <property type="entry name" value="HDAC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000014">
        <property type="entry name" value="Histone deacetylase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0437670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="404" mass="43501" checksum="03513F9C7BB50DBB" modified="2022-05-25" version="1">MLLHSEMETKPNPHPERPNRLRAIAASLAAAGIFPSKCALVPPREITKEELVMVHTSDHVESVEQTKNMLYSYFTSDTYANGHSACAAKLAAGLCADLASLIVSGRVRNGFALVRPPGHHAGVKQAMGFCLHNNAAVAALAAQKAGAKKVLIVDWDVHHGNGTQEIFEGNKSILYISLHRHEDGSFYPGTGAADEVGVLDGKGFSVNIPWSCGGVGDNDYIFAFQHVVLPIATEFAPDITIISAGFDAARGDPLGCCDVTPAGYSQMTSMLTACSEGKLLVILEGGYNLRSISSSATEVVKVLLADGPSYGTDAAAPSKEGMQTVLHVLDIQRKYWPVLVPIFASLQAQQGQTSSKYANAENKLKRRMLMGGPGPVWWKWGSKRLLYEILFEGRRPRKSKARGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YSE6</accession>
    <name>A0A8I6YSE6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="216" mass="25108" checksum="6C222B0EC7273E82" modified="2022-05-25" version="1">MPTKFLKVLAHGTTKLDVVYTNESREVPFFLQQLKEKWFDDTMEHEKFLGLDLEYTADQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSGITFASVDIRNGKLKMRHSFGIEIPAGCLVDLQTVFRLRHERTSMAHMAVALIDEEYGDMKTSFPKSQHKLWEKGPLDPINIEYGAKYAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="66" mass="7442" checksum="4F62ADF8E3D8BC62" modified="2022-05-25" version="1">MGGSMSCFGGSGRDYDEAYEQPRRSSRKVRPSDEDGLWYVGERDVDRKATEFIARFHASASYVEAT</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
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        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YYU5"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF192">
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    </dbReference>
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        <location>
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        </location>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Poaceae</taxon>
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            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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    </evidence>
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        <source>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <begin position="105"/>
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</entry>
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        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Hordeum</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
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    </reference>
    <reference evidence="7" key="3">
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                <consortium name="EnsemblPlants"/>
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            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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</entry>
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    <accession>A0A8I6Z9F7</accession>
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        </lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.7HG0623200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0751300.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409627"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="entry name" value="DUF4378"/>
    </dbReference>
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        <property type="entry name" value="OS06G0728200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40836">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="570"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="414"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
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        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="745" mass="81407" checksum="52DCEF6D3CC3E527" modified="2022-05-25" version="1">MLGQPSVIARLMGMEDHHQTTATAVVHASEGTTTVISLLKPASSQVEQPRCGPLGSHHGRRIGGDYYRYCLNKMKPGRRRRARRDGRRREHPQEELLDKIKEDFRASWQLASTATPPLGTEDEETVADASGRRRSGDRVAGLLDGRCIQRIAQENLRREKMARYGYGGRRSSVEEEEDGVKALVVGLKKKNAEEEAEQLHGGSEAGASEEFSGDHDQMSSTSSEKSRGMPATRIAILRPTTGTSTRAVAGDHRNAPPSWKAVRDGGMDMEDFLREVKERAERLKVETEAKADGPGDVVAKARKGWGSVGDDPAGTAQDTARQIREAVGKRLSRLESFRVFRGDRSRRDGAAASPSPEHRMLKSVMARTEGMSPTKITGRGPRVSRWSPLRAGSGVNDRQSPDRDPRRSPAKLHDDGIDFVAASPRALLRSFSAPAAGCSDASSVGAGKLVDRCRSLSIFRGTVASLRQSLCLGGGAWKLLLMHLSKKKASSPASPRQNLLAGLAPPSPVSPLEVSGGRHFSGDLNCTFLPESSPRWSPRCSSEFEASVGGGESPWERKADAAAENDDPDTAYVREVLVAAGLFNDDDGDTAASRTDSIVISEEVFDEVEDGYYYRHLRRRDDGAGEDEELGAADRRRLLFDLANEALLAGARPAVSYSSPYCLRRWVVECNNGAPSSSRLRGRELEDEVWRHVAMVTVDYTAEREVGRSPWVPEALCEDACAVGRKIERAIFDELLGDVVRQLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0G2</accession>
    <name>A0A8I7B0G2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123405541</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>oxalate + O2 + 2 H(+) = H2O2 + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:21880"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
            <dbReference type="EC" id="1.2.3.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Secreted</location>
            <location evidence="2 16">Extracellular space</location>
            <location evidence="2 16">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the germin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955132.1">
        <property type="nucleotide sequence ID" value="XM_045099197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0G2"/>
    <dbReference type="SMR" id="A0A8I7B0G2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010980"/>
    </dbReference>
    <dbReference type="GeneID" id="123405541"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0008150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0008150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0008150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0010980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405541"/>
    <dbReference type="OMA" id="YIALFAM"/>
    <dbReference type="OrthoDB" id="589868at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050162">
        <property type="term" value="F:oxalate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 13 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338972" description="Germin-like protein" evidence="16">
        <location>
            <begin position="21"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="17">
        <location>
            <begin position="59"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="215" mass="23071" checksum="71A9B6E3CA5AD99F" modified="2022-05-25" version="1">MASKFILLALLALSFSRTLASDPGQLQDFCVADRTSQVFVNGFACKDPKTAVVEDFFFSGLHMTGNTSNKQGSAVTAVNVAQIAGLNTLGISLARIDFAPYGQNPPHIHPRASEILTVLEGSLYVGFVTSNPENRLFSKVLNKGDVFVFPQGLIHFQFNTGDKEAIAIAALSSKNPGVITIANAVFGSKSSISNDILAKAFHVDKRIVDHIQAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0K1</accession>
    <name>A0A8I7B0K1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044955987.1">
        <property type="nucleotide sequence ID" value="XM_045100052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0K1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0018170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0018170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0018170"/>
    </dbReference>
    <dbReference type="GeneID" id="123407019"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0014110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0014110.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0014110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0018170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0018170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0018170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407019"/>
    <dbReference type="OrthoDB" id="690715at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF32">
        <property type="entry name" value="CASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0018170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="296" mass="30747" checksum="E4896E92ED3D28A8" modified="2022-05-25" version="1">MDWAPDQKALGGLGLAGVCRETCRVIHRVMLPNFVHLGPPLLSAALLLANSAAVRALSDLDHCPSLLADRLAFSLFATVSVGAGLLLLLLCAAAYAFCVASLYSTGGDLLAADRVLRGFPTAPLARLACTFLILAAASFLLVHASLSFAALFHPQELVSAPGKTVLPVRLLAWAACLAGAGYVAVVCQLACVVSLLEDAVLLGALRKSRALLAGKFWAAAGVFVTLDGCIFAVLVAFQALVVDDALGLGLGFQVAAGVVMAVALCAVVLLTLVAQPVVYMVCKNHHYQVVHKVHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2K7</accession>
    <name>A0A8I7B2K7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123425678</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044965320.1">
        <property type="nucleotide sequence ID" value="XM_045109385.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I7B2K7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134530"/>
    </dbReference>
    <dbReference type="GeneID" id="123425678"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0110830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0110830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0110830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0134530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425678"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000006">
        <property type="entry name" value="HOPM interactor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000104">
        <property type="entry name" value="HOPM interactor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF312">
        <property type="entry name" value="BREFELDIN A-INHIBITED GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="550"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="517"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1405"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1467"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1594"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1405"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1594"/>
            <end position="1611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1715" mass="189695" checksum="0D072AE2204339EB" modified="2022-05-25" version="1">MAGAAGGFVTRAFEAMLKECSANRGKFAALQQSIQSYLDAIKGAAAQEQQEDGAPAPVTHVLASAGRVLEGTQAELVLQPLRLAFETKHIKLVEPALDCLHKLVAYDHLEGDPGLEEGKNSPLFTDILNMVCGCVDNTSSDSTVLQVLKVLLNAVASNRFRVHGEPLLGVIRVCYNIALNSKSPVNQATSKAMLTQMISIVFRRMESEQASVPPASSAVKDAPSSSTKEDSENGEISTDKQDEEKTTLGDALSMNRASEASPTSVEELQNLAGGADIKGLEAVLDKAVELEDGKKVSGGIDLDTMNIIQRDALLLFRTLCKMSMKEESDEVATKTRLLSLELLQGLLEGVSDLFAKNFHFIDSVKAYLSYALLRASVSSSPVVFQYATGIFSVLLLRFRESLKGEIGVFFPLIVLRSLDSSDSSLSQKSSVLRMLEKVCKDSQMLADMFVNYDCDIEGPNLFERMVSALSRIAHGSQSADSAAVASSQTVSVKGSSLQCLVSILKSLVDWEQARRDSSNHGSVAESHDDDASARSLATDETKVQEDGRNQFERAKAHKSTMEAAISEFNRKPAKGIEYLLSNKLIENKASSVAQFLKSNSSLDKVMIGEYLGQHEEFPLAVMHAYVDSMKFSGLKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYILAYAVIMLNTDAHNPMVWPKMSKSDFVRLNTVSDEEECAPKELLEELYDSIINEEIKMKDDLHAAKTSKIRPEIEEKGRLVNILNLALPRLKSASDTKAESEKIIKQTQAVFKNQGQKRGVFHVAQQVELVRPMLEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFKAGIHLTRVLGMETMRYAFLTSLVRFTFLHAPKDMRSKNVEALRTLLALADTDMDALQDAWNAVLECVSRLEYITSSPSMAATVMQGSNQISRDSVVQSLKELAGKPAEQVFVNSVKLPSDSIVEFFDGLCSVSAEELKQTPPRVFSLQKLVEISYYNMARIRLVWARIWSVLAQHFIAAGSHHDEKVAMYAIDSLRQLGMKYLERAELNKFTFQNDILKPFVILMRNSRSEKIRGLIVDCIVQLIKSKVGSIKSGWRCVFMIFTAAADDENEYIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIGFANNKCTPRISLKAIALLRICEDRLAEGFIPGGSVKPVDVLPEANFDVTEHYWFPMLAGLSDLTLDSRPEVRHCALEVLFDLLNERGHKFSSPFWESIFHRVLFPIFDHVRHAGRDGLSSGDDWLRDTSIHSLQLICNLFNTFYKEVSFMLPPLLGLLLECAKKTDQTVVSIALGALVHLIEVGGHQFSDGDWETLLKSIRDASYTTQPLELLNSLGFQKTNNQHLLPRESETDASSYHDIREGEASISNNGEQEGHQETSPQIGLESSEGLPSPSGRAQPAISPPNQTFGQRFMGNMMGNLLVRSLTSKSKAKMDDAAPPSPAKTPDADGADKTEEEENPMMETVRSKCITQLLLLGAIDSIQKRYWSKLQATQQIAIMDILLSLLEFASSYNSPSNLRTRMHHIPPERPPLNLLRQELAGTTIYLEILHKSTVHNGANGSSEETNGSGVESNQQEKLKSLAEGKLVSFCGNILKEASDLQPSNGETASADIHRVLDLRAPVIIKVLNGMCIMDAQIFKKHLREFYPLITKLICCDQMDVRGALGDLFSKQLTPLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3X6</accession>
    <name>A0A8I7B3X6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3X6"/>
    <dbReference type="SMR" id="A0A8I7B3X6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0163950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0163950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0163950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0197450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48222:SF5">
        <property type="entry name" value="INHIBITOR I9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48222">
        <property type="entry name" value="PROTEINASE INHIBITOR, PROPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152966" evidence="1">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="11999" checksum="D6D7C6BA06EF33A8" modified="2022-05-25" version="1">MAKPTSQSVAAIAILMLAVATLAVPQAPPKRSGQEIHLFEVTVGLVDGADDDYNYRLLAAVLGSVEAARSVTFETYPGTFSAFLTNNQARRLSRVKGVLGVRQRDDPVPTGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4F6</accession>
    <name>A0A8I7B4F6_HORVV</name>
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        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123442341</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="13" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4F6"/>
    <dbReference type="SMR" id="A0A8I7B4F6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218240"/>
    </dbReference>
    <dbReference type="GeneID" id="123442341"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0181550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0181550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0181550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218240.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123442341"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HD"/>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Leu_zip_homeo"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="68"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
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            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
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            <end position="161"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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        </source>
    </evidence>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="210" mass="23367" checksum="8C989003A71FD0DF" modified="2022-05-25" version="1">MYMVQEEAGALALGLSLGSGAGHHPLEQKEAATLEPYLSLSLAGAGAGAVKKEVERALVYCSTDDQVEGYNRKKLRLTKEQTALLEDHFKEQSTLSHKQKSSLARQLNLRPRQVEVWFQNRRARMKLKQTEVDCELLKRCCETLTEENRRLHRELQHLRALHHHHPTAAFFIPSTATASVCPSCAGAPPVHAADRPATKRSFFATKSAAC</sequence>
</entry>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
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            <strain evidence="1">subsp. vulgare</strain>
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0112890"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0112890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="8888" checksum="2A08C4BE54A0BAB1" modified="2022-05-25" version="1">MRGLIRTAVINIHMEIVRTVDISVLQTYEDHHAIAIWLVADKLTSIQTESINIRRSVLVQSCNEINTATWRSNSRIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B541</accession>
    <name>A0A8I7B541_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975567.1">
        <property type="nucleotide sequence ID" value="XM_045119632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B541"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0250180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0250180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0250180"/>
    </dbReference>
    <dbReference type="GeneID" id="123443298"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0207760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0207760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0207760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0250180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0250180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0250180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443298"/>
    <dbReference type="OrthoDB" id="2274046at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF5">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="1">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0250180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="145" mass="15403" checksum="4A7D021D81FBB158" modified="2022-05-25" version="1">MSTAAAQGGEKPALRKPVFVKVDQLKPGTNGHTLIVKVVSATPVPGRVRTGAPASSSARAPRIAECLVGDETGSIIFTARNDQVDLLKAGATAILRNAKIDMFKASMRLAVDKWGRVEATEPASFTVNEENNLSQVEYELVNVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6S2</accession>
    <name>A0A8I7B6S2_HORVV</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426963</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966821.1">
        <property type="nucleotide sequence ID" value="XM_045110886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6S2"/>
    <dbReference type="SMR" id="A0A8I7B6S2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174250"/>
    </dbReference>
    <dbReference type="GeneID" id="123426963"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0144020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0144020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0144020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426963"/>
    <dbReference type="OrthoDB" id="1432093at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF22">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE PNLDC1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="734" mass="81173" checksum="DE39DE9A33B4281A" modified="2022-05-25" version="1">MQCRRRLAPLLRAPPPKSLLPRLLSRLVSSSSPSPPPSGGGGDEAGDGSVAGVAVKQVTRGNLAEALEELRARVRDAAFVGIDLEMSGVTSAPWRDTFELDRADVRYLKLRDSAKRFAVLQLGVCPFRWDPAKSAFVAHPHNFFIFPRKELPYDSSSHEFLCQTTSLDFLAKHQFDFNTCFHEGISYLSRAQEEEALQKLNVQHHDGISACISKQEDVQLKSTADLLFTERMKIRFNEWRDAISTNPRVDNHKLGSNKFSTDQFQTVFFKMRPAIMLDGFTSHQLKLIQQVLRKNFRDLMYICTFGEDGTSEKRVVYTDNNEDSILLMKDVQDDLLRSRKAKVKSAIGIRHVIDLIASEKKLIVGHSCFLDIAQVYSKFIGPLPSSIKEFALAINRIFPYIVDTRHLMSAGDAVQRLMRQKSKSLSSAFSLLCPAFHPTAPEASILAPVKIEVEADETTLSCFASGAKHEAGYDAFMTGCVFAQLCPHLGVKFEDLTLKESLEMNNKLKKYINLLPPSYNSGTVLDLSTGMERPDAGCKLRYPAAVYDNIVLIWGFQSGLSAKDIKACICKVFGPASVTTIFSIDSTAVLVQFSKQESVNDFLDLKATLEKQDSAISVLHPLSTVLEGGQTRASNYDTYIGICSSSVTKYLFADQANAVCSASNTELRGENNVDASDAPGTNSVLDENVHTSVKQAEGSKHGSNKQDATDISCQDILDALQDGKTLFGKQTRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6Z3</accession>
    <name>A0A8I7B6Z3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6Z3"/>
    <dbReference type="SMR" id="A0A8I7B6Z3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0351360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0351360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0351360"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0351360.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0351360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15710" checksum="26FB8A82BA929EF3" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSEVEVCEPEIPTHIKILVEKLNLVEKENKYLEKKLKIIEDKNMELELHVADVIDDRKMKIDAMQLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B974</accession>
    <name>A0A8I7B974_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B974"/>
    <dbReference type="SMR" id="A0A8I7B974"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="160" mass="18512" checksum="72D87950E69D0159" modified="2022-05-25" version="1">MCRVPSHAPPNRRRSPVPISSPAPPCAMVLVSVLVGPRPAYDSDVDVCEPEIPTYTLVERLNLVERENNYLKKKLKIIEEKNMELELHVADVIDDHKIKMDAMRLKMYAMRLKMDAMRLKIRNIRKYAIDKEDWYHYVVGSIVTLVAILITFVVAFKCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCD3</accession>
    <name>A0A8I7BCD3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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            <strain evidence="2">cv. Morex</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="7">subsp. vulgare</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="E3 ubiquitin ligase BIG BROTHER-related"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="E3 UBIQUITIN LIGASE BIG BROTHER-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47530:SF3">
        <property type="entry name" value="OS08G0548300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="95"/>
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        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0687450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="292" mass="31489" checksum="78B47F7CAD22E2AB" modified="2022-05-25" version="1">MEGPKGNGGEKPSADQNHSPNTPAAAGAGGDDVSAAAAAAVGRRPFTALSQEEADLALARVLQEQERAYMMLSAHGGDGSEYDASDAGSYDYYDEEEEGIEGDKGSDYEEEGVLPNDDDEEVGDAEGAELDPARYEDDEAYARALQDAEEREVAQRLMALAGITDLGEEMEHDEEDEEDGDSAQDAWEDVDPDEYSYEELVALGEVVGTESRGVSADTLASLPSVTYQAQDKQESNMEQCVICRVEFEEGESLVALPCKHSYHSDCINQWLQLNKVCPMCSAEVSTSDNNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEQ0</accession>
    <name>A0A8I7BEQ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4283 domain-containing protein</fullName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEQ0"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33170">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33170:SF22">
        <property type="entry name" value="OS10G0417100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0487340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11322" checksum="8B8C3FF9EE6DEFCF" modified="2022-05-25" version="1">MEVAWVKISNVPLDKRSERNMAYVASLVGVPLEIDSATLHRPASARVKVGCRNVDDIPSVAEVVLGEHFYDFYFEVDQVLVRDPNRGEFLLSSGKSNDKEK</sequence>
</entry>
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    <accession>A0A8I7BID3</accession>
    <name>A0A8I7BID3_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC123407748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956884.1">
        <property type="nucleotide sequence ID" value="XM_045100949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BID3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655170"/>
    </dbReference>
    <dbReference type="GeneID" id="123407748"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="903824at2759"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055276">
        <property type="entry name" value="NHL41-like"/>
    </dbReference>
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        <property type="entry name" value="2, PUTATIVE-RELATED"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
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            <begin position="48"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="34244" checksum="0A1FB91CD01CC2E7" modified="2022-05-25" version="1">MMHGKSDSDITSLAASSPPRSPKRAGGYYVQSPSRDSAHDGGGAGGYKSSSMQATPVYNSPNESPSHPSYGRHSRASSVSRFSGILRGGSGRKGGGGGGGDLKAVNAKGWPECSVIEEEGSYEGLSGGDSGLSGRCKVALGFVSFLLLFTVICLIVWGAARPYEPDVVVKSIAMDNFYAGEGTDHSGVPTKMVTLNCSLNMIVYNPASMFGIHVSSGPVRLLYSEIAIGVGQVRKYYQPSRSHRLVSAVIHGEKVPLYGAGGGLSLSGNDGATVPLTVDFELVSRGYVIGKLVRVTHRAHVTCRITVDAKRARTRIPKKACAVYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJF3</accession>
    <name>A0A8I7BJF3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJF3"/>
    <dbReference type="SMR" id="A0A8I7BJF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0708010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0708010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0708010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0708010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="16057" checksum="404AE015711A2A6B" modified="2022-05-25" version="1">MVLVSAPVGPRPLYDSDVEVCEPEIPTHREMLLEKLTLVEKENKYLKEKLKIIEYKNMEMELHVVEVIDDRKMKIDAMRLKMDAMRLKMHETRLKIRNIRKYDIDKEAWYHYAVGSIVTLLVILIAFVVAFKYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQ90</accession>
    <name>F2CQ90_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123449187</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK353792">
        <property type="protein sequence ID" value="BAJ85011.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982243.1">
        <property type="nucleotide sequence ID" value="XM_045126308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQ90"/>
    <dbReference type="SMR" id="F2CQ90"/>
    <dbReference type="PaxDb" id="4513-MLOC_73194.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392970"/>
    </dbReference>
    <dbReference type="GeneID" id="123449187"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0326920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0326920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0326920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0392970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449187"/>
    <dbReference type="eggNOG" id="KOG3387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_1_1"/>
    <dbReference type="OMA" id="IKNQIYA"/>
    <dbReference type="OrthoDB" id="748723at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CQ90">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21104">
        <property type="entry name" value="SNU13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="24"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ85011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="128" mass="13900" checksum="B2071ED98F9E13AC" modified="2011-05-31" version="1">MSQETVNPKAYPLADAQLTITILDIVQQAANYKQLKKGANEATKTLNRGISEFVVMAADTEPLEILLHLPLLAEDKNVPYVFVPSKQALGRACGVTRPVIACSVTSNEGSQLKQPIQSLKDAIEKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F2CV90</accession>
    <name>F2CV90_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440117</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WEB family.</text>
    </comment>
    <dbReference type="EMBL" id="AK355542">
        <property type="protein sequence ID" value="BAJ86761.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK360453">
        <property type="protein sequence ID" value="BAJ91662.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972631.1">
        <property type="nucleotide sequence ID" value="XM_045116696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CV90"/>
    <dbReference type="SMR" id="F2CV90"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0281510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0281510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0281510"/>
    </dbReference>
    <dbReference type="GeneID" id="123440117"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0233450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0233450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0233450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0281510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0281510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0281510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440117"/>
    <dbReference type="OMA" id="KRCVIAE"/>
    <dbReference type="OrthoDB" id="685331at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CV90">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009904">
        <property type="term" value="P:chloroplast accumulation movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009903">
        <property type="term" value="P:chloroplast avoidance movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008545">
        <property type="entry name" value="Web"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054">
        <property type="entry name" value="HEAVY CHAIN, PUTATIVE, EXPRESSED-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054:SF2">
        <property type="entry name" value="PROTEIN PLASTID MOVEMENT IMPAIRED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05701">
        <property type="entry name" value="WEMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="560"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ86761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0281510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="620" mass="68567" checksum="A5AA2B2661A524F8" modified="2011-05-31" version="1">MEATMDGGSVRATMSIFGESISGRKADKNRAQEKLSSEMRQVAKSDMDKLNERKASVDNERAGAESELSRARAMAKELERQIDQTKAKATSQRSGLQLQATWARKNGAEEASDAQYAEVSQELDRVKRELRKLKLEVKSAAEAKAKAESDVVATVCKIQSNLQAADEMKRRVDEANEEHVLVELARIEAERERRELEAQHVAEAERFAREIEAARAKVKEARREVSRARELEAKLEDTNADVEVLQGEMELVRAMEKNHVPNDGAAEATATARQKKEEAEDRALLQAAEAELSVAKNELESIKAGAFQFMTSMDRTRTEIMGVVQEIDRLKAQEKNADAQVQQLNAKLLKARAQMEAVTAADERSKAIVSNLTAAMQQLHAETEAASKEEELTMLEKRCVVAEADNVAAEMATAEERIRQSVKELEAAKASEASAMKKLKAAVESTMQARASAAPRRQGTITVSRFEYEYLSGRAALVRVVADKKVAAAQAWVQALKASEKEVAMRAEAAEREMREMGPREAQAASEAEKTAGEQKALEQELYDLNATAESIGLQCAYPRRRSSRVSATSRRSKGRRSSVSAANWNPKSPSFTIKRKKKMMPSLLKLIKEKRGGSDKSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYC3</accession>
    <name>F2CYC3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444654</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356627">
        <property type="protein sequence ID" value="BAJ87844.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359315">
        <property type="protein sequence ID" value="BAJ90526.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359480">
        <property type="protein sequence ID" value="BAJ90689.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977386.1">
        <property type="nucleotide sequence ID" value="XM_045121451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYC3"/>
    <dbReference type="SMR" id="F2CYC3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070840"/>
    </dbReference>
    <dbReference type="GeneID" id="123444654"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0057120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0057120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0057120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0070840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444654"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009579">
        <property type="term" value="C:thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042549">
        <property type="term" value="P:photosystem II stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="237"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ87844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="594" mass="63733" checksum="472E4D0DAC42F12A" modified="2011-05-31" version="1">MASSSLLPPPTFANKYHALLHPHGARHRLTRCGAAAADGGPGDELLSSALHLGRPDPVVATVVSDTGTDGWGDLFTELKRSLQSDSSDPAASGAASIGAAVPDELLSSPPASEAGNAGAALLVPDEVLTSASDMVTTDGVGAAVDAIPDELLGALHLDASSPAVRVADGALSQLEELTAGMNEAERWALFGIVAVTWLYLTARPGVLSGAVDTYVLAPLQQALDTVLGRRSLTMSDFVVGERIGEGSFGVVYSGAVVPRGGPAIEERAGKAKTKLQLDDRYKEKVILKKIKVGTVGAKECGDYEEWFNYRVARAAPESCADFMGSFVADKTKSEFVKGGKWLVWKFEGDRTLGNYVTDRGFPSNLEPLMFGRALRGVDSLTRAALVVKQVMRQLVTSLRRIHDTGIVHRDIKPSNLVVTRRGQVKLIDFGAATDLRIGKNYVPDRTLLDPDYCPPELYVLPEETPTPPAEPIAAILSPILWQLNSPDLFDMYSAGIVLMQMATPALRSSSGLKNFNSELKAAGYDLNRWRETTRRRPDLQILDLDSGRGWDLATKLIAQREKGRLSAAAALRHPYFLLGGDRAAAVLSKLQLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYZ8</accession>
    <name>F2CYZ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123447858</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="AK356854">
        <property type="protein sequence ID" value="BAJ88069.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980496.1">
        <property type="nucleotide sequence ID" value="XM_045124561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYZ8"/>
    <dbReference type="SMR" id="F2CYZ8"/>
    <dbReference type="PaxDb" id="4513-MLOC_56093.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0344830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0344830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0344830"/>
    </dbReference>
    <dbReference type="GeneID" id="123447858"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0287840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0287840.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0287840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0344830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0344830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0344830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447858"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="606096at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CYZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1046">
        <property type="entry name" value="OS11G0226933 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="195"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="436"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="566"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ88069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0344830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="914" mass="104834" checksum="85D39029CFFEAB01" modified="2011-05-31" version="1">MAEAVLIVVTKIGDILLEEATKSVIAKISEKVTNLKELPVKVEEIKKQLTMMGNVIRQIGTVYLADDVVKTWIGVVRKVAYHVEDVIDKYSYHLLQLQEEGFLKKFFIKGTHYVKVFSEITDEIVEVENEIQLVIKMKDQWLHPFQLAANPLTEMERQRSQDSFPEFVKDGDLVGIEKNRIWLTRWLYSEEPETETENTVITVSGMGGLGKSTLVSNVYEREKINFPAHAWIVVSQVYTVDALLRKLLWKIGYTEQPLSAGIDKMDVHDLKKEIQQRLKNRKYLIVLDDVWEQDVYFQIHDALQDLPGSRIIITTRKDHVAGISSPTRHLELEPLSKSDAFDLFCRRAFYNQKGHICPKDFETIATSIVDRCHGLPLAIVTIGGMLSSRQRLDIWTQKYNQLRSELSNNDHVRAIFNLSYHDLPDDLKNCFLYCSLFPEDYQMSRESLVRLWVAEGFVVRKEKNTPEMVAEGNLMELIHRNMLEVVENDELGRVNTCKMHDIVRELAIIVAKEERFASADDYASMILVQQDKDVRRLSSYGWKNDNVVKVKLPHLRTVLLLEAISPCSGILPSILSESNYLAVLELQDSEVTEVPTSIGSMFNLRYIGLRRTKVRSLPDSIENLSNLHTLDIKQTKIEKLPRGVVKIKKLRHLLADRYADEKQTEFRYFIGVQAPKELSNLEELQTLETVESSSDLAEQLKKLMQLQSVWIDNISADDCANLFATLSTMPLLSSLLLSARDANEALCFESLKPSSSYLHRLITRGQWAKGTLNSPIFLSHGKNLKYLAISWCHLGEDPLGMMAPHMPNLTYLRLNNMHSVKTLVLSKDSFPNLKTLVLRHMHDVSELKIIDGALPCIDGLYIVSLLKLDKVPQGIEFLRSLKKLWLLNLHKDFRTQWDTKGMHQKILHVPEVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F2D1Q7</accession>
    <name>F2D1Q7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402872</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357814">
        <property type="protein sequence ID" value="BAJ89028.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952766.1">
        <property type="nucleotide sequence ID" value="XM_045096831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D1Q7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598930"/>
    </dbReference>
    <dbReference type="GeneID" id="123402872"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0496470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0496470.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0496470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0598930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402872"/>
    <dbReference type="OrthoDB" id="1026046at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF336">
        <property type="entry name" value="OS02G0643800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ89028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="188" mass="21142" checksum="A187B5E2525809A1" modified="2011-05-31" version="1">MMARRQRGFRLGRKLLGLWRWALCNRRRRRGGGYARLQRCQLGAGAKPFNTAVGSAKQQQQQQLVVLPRELDEPRRRMLGWGRSLARRMRLLPRRGERLLEEAGEATTPKGQVAVYVGGDEPGGESMRYVVPVVYFNHPLFGELLREAEEEFGFEHPGGITIPCAATRFERAAAMAAAGGGRKAPGWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2C4</accession>
    <name>F2D2C4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123403237</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Lysosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK358031">
        <property type="protein sequence ID" value="BAJ89245.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953118.1">
        <property type="nucleotide sequence ID" value="XM_045097183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2C4"/>
    <dbReference type="SMR" id="F2D2C4"/>
    <dbReference type="STRING" id="112509.F2D2C4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0625910"/>
    </dbReference>
    <dbReference type="GeneID" id="123403237"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0519350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0519350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0519350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0625910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0625910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403237"/>
    <dbReference type="eggNOG" id="ENOG502QQST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="726732at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D2C4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000023">
        <property type="entry name" value="heparanase-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363:SF17">
        <property type="entry name" value="HEPARANASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363">
        <property type="entry name" value="HEPARANASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090607" evidence="11">
        <location>
            <begin position="34"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ89245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="549" mass="58519" checksum="E754F4FADF1A34DE" modified="2011-05-31" version="1">MAAGLLLPQAARLCFSFWAVAVAVLSLAGSSAAANGTSAGAGAAGEAAVVVDARAAVAETDSAFVCATLDWWPPEKCDYGTCAWGRAGLLNLDLSSKILQNAVKAFSPLRLRLGGSLQDLVVYGTGDTGAPCSPFTKNTSAMFGFTQGCLPMHRWDELNAFFQESGAQIVFGLNALNGRVPMPDGSMGGPWDYTNAASFIQYTVSKGYDIYGWELGNELSGSGVGTRVGADQYAADVINLNQVVDKAYQGSKPLVIAPGGFFDAGWFTELVAKTKPNQMDVITHHIYNLGPGVDTHLVEKILNPSYLDNMVSTFSNLQGILKSAGTATTAWVGEAGGAYNSGHHLVTDAFVFSFWYLDQLGMSAKYDTKSYCRQTLVGGNYGLLNTTTFEPNPDYYSALLWHRLMGTKVLSTTFNGTNKIRAYTHCTKDSEGITLLLINLSGNNTNHIYVTSEGAQEGAHPHGAKEHTRSIPKLGETAGLTRQEYHLTAKDGNLQSQHMLLNGNVLATGANGAIPKLEPVQVDGTQPITLGPYSIVFAHIPDFNAPACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2D9N5</accession>
    <name>F2D9N5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123406997</name>
        <name evidence="10" type="synonym">LOC123406377</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK360597">
        <property type="protein sequence ID" value="BAJ91806.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372915">
        <property type="protein sequence ID" value="BAK04112.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955954.1">
        <property type="nucleotide sequence ID" value="XM_045100019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D9N5"/>
    <dbReference type="SMR" id="F2D9N5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0552230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0552230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0552230"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557640"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0733020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0733020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0733020"/>
    </dbReference>
    <dbReference type="GeneID" id="123406997"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0463280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0463280.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0463280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0552230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0552230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0552230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0557640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0733020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0733020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0733020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401278"/>
    <dbReference type="KEGG" id="hvg:123406377"/>
    <dbReference type="KEGG" id="hvg:123406997"/>
    <dbReference type="OrthoDB" id="1846915at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D9N5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ91806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0552230.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0733020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="381" mass="42680" checksum="EBA4CB1176707C58" modified="2011-05-31" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F2DAQ6</accession>
    <name>F2DAQ6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410429</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AK360970">
        <property type="protein sequence ID" value="BAJ92177.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959310.1">
        <property type="nucleotide sequence ID" value="XM_045103375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DAQ6"/>
    <dbReference type="SMR" id="F2DAQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734470"/>
    </dbReference>
    <dbReference type="GeneID" id="123410429"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0609340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0609340.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0609340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0734470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410429"/>
    <dbReference type="eggNOG" id="KOG0869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066247_13_1_1"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DAQ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000035">
        <property type="entry name" value="Nuclear transcription factor Y subunit B-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="6">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ92177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734470.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="173" mass="18834" checksum="E36B7EABF1092E62" modified="2011-05-31" version="1">MPDSDNDSSGPSNAGGELSSPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVEPLKYYLHKFREIEGERAAPSTTTTTASTTPPHLPEMPRSNNAGGYAEYGSGATVPAPGGMMMMGLPTARRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2DBU1</accession>
    <name>F2DBU1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="AK361355">
        <property type="protein sequence ID" value="BAJ92562.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK370875">
        <property type="protein sequence ID" value="BAK02073.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DBU1"/>
    <dbReference type="SMR" id="F2DBU1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0505720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0505720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0505720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0609900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609900"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076228_0_2_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DBU1">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000002">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00492">
        <property type="entry name" value="RHOGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ92562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="245" mass="26598" checksum="F90DB25C8688CBB0" modified="2011-05-31" version="1">MSSAVEAISCSKVAAAAAGPEEAKECAAVAEHGSGNGNGGGDAAPHCGKGVEEEDEEDGEKVPEHIDLGPILSIKDQLEKDKDDESLRRWKEQLLGSVDLNSVGETLEPDVKIMSLSIQSPGRPDIFLPLPAEPSGKGVWFTLKEGSPYKLKFTFSVSNNIVSGLRYTNTVWKTGIKVDSAKEMLGTFSPQPEPYAYVTPEETTPSGMFARGSYSAKTKFLDDDRKCYLQINYTFDIRREWPSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F2DCH7</accession>
    <name>F2DCH7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123398676</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK361594">
        <property type="protein sequence ID" value="BAJ92798.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK373749">
        <property type="protein sequence ID" value="BAK04946.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DCH7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0476460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0476460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0476460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0394670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0394670.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0394670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0476460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0476460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0476460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039609">
        <property type="entry name" value="VQ_15/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179:SF76">
        <property type="entry name" value="OS09G0370500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="2">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ92798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK04946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0476460.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="43289" checksum="AC991DA023840ADD" modified="2011-05-31" version="1">MDSGNSGSLQSSSGGDDDFDSRGGGVDSSPLSALLRPTPSPSSAGAFSMHGSLYGLQDFTSASPQQQQQQLQHQQHHHQQQQASWSAQFMAGPASSSTRVAPPDAGMGASAHQAPDPSAAAAAAPPRGSRKRARASRRAPTTVLTTDTSNFRAMVQEFTGIPNPPFAAGAGGPGASFNSRFDHIFSSSALRSAAGAAGDPASSLPPYLLRPFAQKLQTATSPFAPFTSPSSSTPPGPSSNMGIANANARAAATAAATTTASANQRGVAGDDFQLTSSALLRMQDHSSNYLSFQNLLGSTQPSSQHMFGGLSHGAPRLHEQSPSEFMPGAGSGSMGLSHGGMMVSEGMHMHQQQRSDVHQGGDELSGVVRAGASGSGGGGHGSCKLNYPPHTVASSSSAAASTDMTPDGGSRSGRGEGLDPWLCTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLL5</accession>
    <name>F2DLL5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123411603</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK364783">
        <property type="protein sequence ID" value="BAJ95986.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960503.1">
        <property type="nucleotide sequence ID" value="XM_045104568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DLL5"/>
    <dbReference type="SMR" id="F2DLL5"/>
    <dbReference type="STRING" id="112509.F2DLL5"/>
    <dbReference type="PaxDb" id="4513-MLOC_74353.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656750"/>
    </dbReference>
    <dbReference type="GeneID" id="123411603"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0656750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0656750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0656750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411603"/>
    <dbReference type="eggNOG" id="KOG2344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010236_2_2_1"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DLL5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF168">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="277"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ95986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0656750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="665" mass="73555" checksum="ED0D5908C9721E32" modified="2011-05-31" version="1">MSAAPPPPPESAATSGGGSDKVLAAAQHIVKSLATSKNAADDMIRILSGFDNRLSSITTDLFPSPDLSSAESPEISSAAAFDAAEQLILLWDATPEALVFEAHEDDIAQYLTAVDVAVEHLARGGAGAGRAGVAVQLAMARLEEELRHHMVRHAVPLDPTGLCFSLRRLSLGSFDDLDFDAATPHSVDATPETARGGPLVSPFDDHSFDPVRPEAVDDLRAIADRMARAGYARELADAYCNIRRDLLDEYLSVLGVERISIDEVQRVEWKQLNDKMKKWVQGVKTVVRVLLAGERRLCDQVLAVSDELREECFVESTKGCIMQILNFGDAVAVCPRSPEKLSRILDMYEALAEVIPEMKDLCLGSSGDGVISDVQAILDRLGEAVRGTLFEFGKVLQQESSRRAMTAGEIHPMTRYVMNYLRLLVVYSETLDGLLDDDGDEGNALERPEDKDQDTEHLESMAPLGRRLLKLMCYLEANLEDKSKLYEDAALECIFSMNNLLYIVQKVKDSELGKILGDHWVRRRSGKIRQYSKSYLRISWMKTLSYLRDDVHGSGSGSGSGSGHSGSRMSIKEKFKNFNLSFEEIYRNQTLWKVPDPQLREELKISISENVIPAYRAFLGRYGSQVDGGRNSGKYIKYTPEDLEGQLSDLFEGSPGSANHSRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2DS83</accession>
    <name>F2DS83_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402986</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK366751">
        <property type="protein sequence ID" value="BAJ97954.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952894.1">
        <property type="nucleotide sequence ID" value="XM_045096959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DS83"/>
    <dbReference type="SMR" id="F2DS83"/>
    <dbReference type="STRING" id="112509.F2DS83"/>
    <dbReference type="PaxDb" id="4513-MLOC_50679.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611370"/>
    </dbReference>
    <dbReference type="GeneID" id="123402986"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0506970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0506970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0506970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0611370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402986"/>
    <dbReference type="eggNOG" id="KOG3410">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098435_1_1_1"/>
    <dbReference type="OMA" id="IASTTHK"/>
    <dbReference type="OrthoDB" id="205403at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013865">
        <property type="entry name" value="FAM32A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282:SF6">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08555">
        <property type="entry name" value="FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ97954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15214" checksum="B9B9E464DDAFDD53" modified="2011-05-31" version="1">MSEYQNVVGGKLKLKGKPLDVKEGGVKKKNKKKHRREESSQGEHGEPREGGSSELPGDPDNEFSEAHKLGDEGNAQADYDHLTPAERRYIEQKQKIDVHKLAKVADKSHRDRIQDFNQYLANLSEHYDIPKVGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F2DWD1</accession>
    <name>F2DWD1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123403030</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="AK368199">
        <property type="protein sequence ID" value="BAJ99402.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952943.1">
        <property type="nucleotide sequence ID" value="XM_045097008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DWD1"/>
    <dbReference type="SMR" id="F2DWD1"/>
    <dbReference type="STRING" id="112509.F2DWD1"/>
    <dbReference type="PaxDb" id="4513-MLOC_81417.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592510"/>
    </dbReference>
    <dbReference type="GeneID" id="123403030"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0491120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0491120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0491120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403030"/>
    <dbReference type="eggNOG" id="ENOG502S4A6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034992_2_0_1"/>
    <dbReference type="OMA" id="IFRAPHE"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DWD1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010208">
        <property type="term" value="P:pollen wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF29">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="250"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ99402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="396" mass="42640" checksum="52F2115994F78C56" modified="2011-05-31" version="1">MALLGNLTASPAATIIRKMRQAERADGPASMLAIGTANPANCVRQDEYADYYFRITQKQHLTKLKSKLSRICDRSAIKKRYFHHTEELLQYHPEFIDRTLPSLDARMDIAATAVPDLAAAAAGKAIAEWGRPAADITHLVVSTYSGAHMPGADLRLASLLGLDPSVRRTMLYLNGCSSGSAALRLAKDIAENNRGARVLVACAELTLILFRAPDEAHVDTIIMQALFGDGAAAVIVGAEPDASAERPIFEMVAAAQSVIPETTHGAVGRLREDGLVFNPSFEMPALIRENIEQCMADAVAPLGLSGGWNDLFWAVHPGGRAILDSVEAGLRLEPKKLTASRHVLSEYGNMSGPTVIFVLDEIRRRREHVVGGHGLGVLLGLGPGVSVETMLLRATW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXC7</accession>
    <name>F2DXC7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123444490</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK368545">
        <property type="protein sequence ID" value="BAJ99748.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977149.1">
        <property type="nucleotide sequence ID" value="XM_045121214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXC7"/>
    <dbReference type="SMR" id="F2DXC7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0291200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0291200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0291200"/>
    </dbReference>
    <dbReference type="GeneID" id="123444490"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0242040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0242040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0242040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0291200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0291200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0291200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444490"/>
    <dbReference type="OMA" id="YTYWDEC"/>
    <dbReference type="OrthoDB" id="10261669at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DXC7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000008">
        <property type="entry name" value="Putative pre-16S rRNA nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317:SF1">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="34"/>
            <end position="139"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ99748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0291200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="22007" checksum="F3F08B8625332945" modified="2011-05-31" version="1">MNYIFKCRRRCAMRLLKAEELFRKLLEGGSKKKARLLGLDVGSKYVGLAVSDQQNRIALPLSVLGRTKTNINLMADDFKTLVNNYSLAGLVVGYPFNLQGQSSPDASQVSLLVGELCKTGKLDDLSYTYWDENFTSKCVEALLNPLNLNDRVETKTMTDKFAAVCILQGYLDNMNRALTSADNVKSNLSSASGQWAFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2E279</accession>
    <name>F2E279_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123412528</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAK01451.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045105478.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0721680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0721680"/>
    </dbReference>
    <dbReference type="GeneID" id="123412528"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0598510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0598510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0598510"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
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        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
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        <location>
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            <end position="124"/>
        </location>
    </feature>
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        <location>
            <begin position="51"/>
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        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK01451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0721680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2E7H5</accession>
    <name>F2E7H5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402496</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAK03297.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952354.1">
        <property type="nucleotide sequence ID" value="XM_045096419.1"/>
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    <dbReference type="SMR" id="F2E7H5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555800"/>
    </dbReference>
    <dbReference type="GeneID" id="123402496"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0555800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402496"/>
    <dbReference type="HOGENOM" id="CLU_130624_0_0_1"/>
    <dbReference type="OrthoDB" id="769720at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CUE_CID7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR041806">
        <property type="entry name" value="CID5/6/7_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038981">
        <property type="entry name" value="CID5/CID6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37252">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37252:SF3">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="89"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK03297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="16435" checksum="528748A036186EEE" modified="2011-05-31" version="1">MERSFKLNPDAAPFVPASKSPFAESLKGKKDPEKQVDETEKSETADKSAGYELPESLSFDDYAESLGKINISTESSSKGEAAEASHAGSHLAVVESLSVMFPDVSADFILEALKANEFDAVLTIDMLSDLCEADDNGHSAEVSGKPQHHDPSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2E8N4</accession>
    <name>F2E8N4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123411679</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK372508">
        <property type="protein sequence ID" value="BAK03706.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960576.1">
        <property type="nucleotide sequence ID" value="XM_045104641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E8N4"/>
    <dbReference type="SMR" id="F2E8N4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667160"/>
    </dbReference>
    <dbReference type="GeneID" id="123411679"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0553550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0553550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0553550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0667160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667160.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123411679"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E8N4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF253">
        <property type="entry name" value="OS06G0234600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="67"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="120"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK03706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="418" mass="47598" checksum="5A9F884BBD0F4B56" modified="2011-05-31" version="1">MRSSTSVGFCTNRRASQQCLEVVRAAPFAFSLIALSVVLLILVGIHDTSSLRSTMFDSTTRKGGDDEKCDMSTGKWVREPRGPIYTNLTCPTIPGGRNCQKYSKDPRHLYWRWQPDRCELPRFAPARFLDLVRGKRLAFIGDSLAGNQMDSLICLLSQVERPTAMYSDASDKIRKWYFPAHDFTVMALTTRFLVHADPVRDNDWKITNSFDIHLDKLDSVWRSRLPELDYAILSGGNWFFRVNYFLEDGRRVGCTGCHDSVADLTDFGVAGGVRRVVRAALEGIARCRGCKRGLVTLLRTYTPSHFEHGTWYDGGYCNRTRPSEEGEVSLKGVEWELRAVQREEVWRAREASGGKRFFEAMDVTKAMMMRPDGHPNWHSVNRKISNDCLHWCLPGPVDMWNEMLLQRLAEISSPAVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2EB22</accession>
    <name>F2EB22_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395100</name>
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    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Poa p IX/Phl p VI allergen family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373347">
        <property type="protein sequence ID" value="BAK04544.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945970.1">
        <property type="nucleotide sequence ID" value="XM_045090035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EB22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533770"/>
    </dbReference>
    <dbReference type="GeneID" id="123395100"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0444080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0444080.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0444080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0533770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395100"/>
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    <dbReference type="OrthoDB" id="661298at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd12805">
        <property type="entry name" value="Allergen_V_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Group V grass pollen allergen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035506">
        <property type="entry name" value="Pollen_allergen/Os"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00833">
        <property type="entry name" value="POAALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81736">
        <property type="entry name" value="Group V grass pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730863" evidence="2">
        <location>
            <begin position="29"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK04544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="217" mass="22430" checksum="0619E5AE1578412D" modified="2011-05-31" version="1">MAMVKNIACTLLLVAIMVAATLVPVATAASEQSIETMDASIKKAFDAVIAAAPKAKRSEAESAVLKQKVAATASLNQAAGDKKKVDEIVTAYKTAANAVLAAKPADKSAVFEKTFGEAGHPATHQAVDSLEDSFKQAIDDVVAAAPSPKQDEMKALVFQQTVLAANSLGQSSMDTKLFAAVTTAYKVASDAVIAASPDDKFPVMEKTFAEAAKVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2ED67</accession>
    <name>F2ED67_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar ATPase assembly integral membrane protein VMA21 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Seed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">COPII-coated vesicle membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the VMA21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374092">
        <property type="protein sequence ID" value="BAK05289.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ED67"/>
    <dbReference type="SMR" id="F2ED67"/>
    <dbReference type="PaxDb" id="4513-MLOC_6954.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0158450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0158450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0158450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0191220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191220"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3YI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2ED67">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03058">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792:SF3">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK05289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="107" mass="11518" checksum="CFFD0A4AC1616C9F" modified="2011-05-31" version="1">MSAVITKFAVTSMVMWMAPVAIMYGFIYQIFPGVGQLSPSAQTMASGFLAVISVNLVIGFYIYMAMKEAPHQEPQPDPTFLANAKASINQPTSSQVSDDSHGKGKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2EFM3</accession>
    <name>F2EFM3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123446696</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Flower</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374949">
        <property type="protein sequence ID" value="BAK06145.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979190.1">
        <property type="nucleotide sequence ID" value="XM_045123255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EFM3"/>
    <dbReference type="SMR" id="F2EFM3"/>
    <dbReference type="PaxDb" id="4513-MLOC_75159.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0391490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0391490"/>
    </dbReference>
    <dbReference type="GeneID" id="123446696"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0325720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0325720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0325720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0391490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0391490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0391490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446696"/>
    <dbReference type="eggNOG" id="KOG1012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042212_1_0_1"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EFM3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000130">
        <property type="entry name" value="synaptotagmin-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF174">
        <property type="entry name" value="OS03G0251600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="15">
        <location>
            <begin position="66"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="246"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAK06145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="512" mass="55591" checksum="B85B01D2EADC2747" modified="2011-05-31" version="1">MGFISGIMMGFIVGVALIAGWAHAMSRRAHKRSAKAAEISALGSLNREDLRKICGENLPQWISFPEYDQVKWLNRQLSKLWPFVEEAATMVIRDSVEPILDVYRPVGISSLKFSRLSLGTVPPKIEGIRVQSFQKGQITMDIDFKWGGDPNIILAVETLVASLPIQFKNLQVFTIIRVVFQLSDEIPCISAVVIALLAEPKPRIDYILKAVGGSLTAMPGLSDMIDDTVASLITDMLQWPHRIVVPLGGVDVDISDLELKPNGKVTVTVVRAESLKNKELIGKSDPYVVLFIRPMFKEKTRVIDDNLNPEWNETFELIAEDKETQHIILEVFDEDSLKQDKRLGIAKVPLSDLEVETVQEINVQLLSSLDTTKVKDKKDRGVLTIRVFYHPYTKEEALRALELEKRTVEERMKTREGTGAVSGAADAASGVASTVTNVAGTGVAAGAAAAGSGVHMAGSGVHMAGSGVAMVGTGIDAFGKSISKASKSISKAVTGPFSSQRRSAASVPTVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F2EGI3</accession>
    <name>F2EGI3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123397819</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Flower</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK375260">
        <property type="protein sequence ID" value="BAK06455.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EGI3"/>
    <dbReference type="SMR" id="F2EGI3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0503110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0417950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0417950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0417950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0503110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0503110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503110"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18868">
        <property type="entry name" value="OS07G0665300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18868:SF35">
        <property type="entry name" value="RRNA N-GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAK06455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="738" mass="81641" checksum="44F7A4001B584DF2" modified="2011-05-31" version="1">MCIETSCSDSEATILTSRRLIPPTRPAATLTIKVRLPNNRIVAGWIEDPKIYVDFFIVNIKNVSGVDAASLDCDIQPAPHTKVAAVYRCFSSGFLTATSGLNLASATSEIIVSTCRIHKAGIGGPLVDFDGNFVGMNSSRTKMEKTAYVRREGIFRFLLFHRKVSVRVKEGADDASRARMNSEMKAIRPSLSAKSVGTLSKSRKGALSKQVISRLSMSVVALTSFDGDTKLRECTGSCIESSCPDATCFLTSINLIPYTYPRRKIAESLTIKVRLPDDQIVTGWIENPELYVHFFIVKIKNVDVINPTHLHHLSLDHATQFEPYRKVAAVWRYYDSGELKTTSGVDLASVFAHTDEKILSTCRIHEVGIGGPLVDFDGNFVGMNYSGTKERKTPYVRRPSVREFMWFRGMVSVKEDVEEAVSARIKGRYGDSAFLRVGAGKYINLLDDMFKNDILRKPLGSFGSEMDRSAYSLASESSKRLASEMNQSVVSLASFNGYAKKFSCTGVFIKSKECSATILTSASLVRVPGSAHMIDNSLWIEVCLPGGFRVVGILNCYNLHYNVALVDIMGFWHPRSIKIHGHPVTSSMDVIAVGSLFSRRILMAAEGKVLIGKQSKLDCKELCVSTCKITKAGIGGPLIDNDGNFVGMNFYEEEETPFLPRDVIHRLLLNLNKKRTDTIVEGVENRWLLPGGTHGNLEIIAAIDGNKWPLPEPRFLGCRDNSSPLHKWPMPRGGRRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>M0UQF8</accession>
    <name>M0UQF8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0UQF8"/>
    <dbReference type="PaxDb" id="4513-MLOC_13115.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0198030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0198030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0198030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0164520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0164520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0164520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0198030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0198030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0198030"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R5ES">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UQF8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045881">
        <property type="entry name" value="MNM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34682">
        <property type="entry name" value="AT HOOK MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34682:SF1">
        <property type="entry name" value="PROTEIN METABOLIC NETWORK MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0198030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="416" mass="44944" checksum="372AF81722AF8C0D" modified="2013-04-03" version="1">MTSEGNKAAAPNEPLPIEPPASSGTKRKRGRPRKYEYSTYEQPHMAQPVQSIPPLRSALYNPNIRHDGVHINHPLGGTLDTKMHTVHVLPAQQTQGYRPSRPVDSGTTVKFRENQASNSSSAHVRGNVGKDVIVGKHFVGKMTKQCPGFSLITVRVKDNQVLRGWIPDETNLRPITPKDDLAPDLPMLQPSQLQKKASAIHRQAAPPLPVHLENVTIAKPLQMRRPVETSTLKFIIPPPAPRPYVGSGVVAAAPVSIISGNVSRPLPKQDTGSLSQEPSATVVPVKSAQPVLVSCRHVDQDVHVEGKSVPEFNSDSESSSGSKDSSGQSPRGSPAAMGETDITSGYKEHSNAANSNQHICKEPSDNPEQSEQPKTEPNILKGVDDPMTGASDDVQSTVKEDELKVDSQEIRLTTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>M0VKJ5</accession>
    <name>M0VKJ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0VKJ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0274700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0274700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0274700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0227660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0227660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0227660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0274700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0274700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0274700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VKJ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        </source>
    </evidence>
    <sequence length="192" mass="22517" checksum="195F07BE20B26711" modified="2013-04-03" version="1">MHTVENCSEITILGHHTGITFSVQQQTEEGRLSFKDKAIKSFIGAGKARPLELSNELFFPSFHERHWFVFLVDLKAWMFIFLDSKYEENNMFHKNIKDMMIDNFIQTWCDSHLRNMGFSQFQTTYPALPKQDDSDSCGIFTLMKMQTYRARNPMQTVFQAKYVPDARIRLAVDLLFSTHNTMDKAKRLVKDL</sequence>
</entry>
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        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045112811.1"/>
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    <dbReference type="SMR" id="M0X5H8"/>
    <dbReference type="STRING" id="112509.M0X5H8"/>
    <dbReference type="PaxDb" id="4513-MLOC_57204.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0218060.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0218060.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="162" mass="17233" checksum="EDFB5A7FFD67BCEA" modified="2013-04-03" version="1">MKQNLAFLGSVVGERPQPLAVREVIKAFLVVLLAGGSGRAFSREDHGAVAEDFAGLKRMFCSCSGDGLVTEEVVETETAAAQGVVDLMASPTGKLIEEFCRLSGGTRGMPPGVLPFVRRHERNAAGHDDDNDNDETVESVGRLHYAACAVPPIETTRQPADP</sequence>
</entry>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045101232.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR34198">
        <property type="entry name" value="OS01G0175100 PROTEIN"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="11879" checksum="3C16503386937FDB" modified="2013-04-03" version="1">MAMAPSASVVSFSARPSAALQPRAASASAGAGGRVRAGAPKGGKWWAPLVGWSGRADYMEAAAPTPVVEEEGRTFVGLTEEKARQLRARMSEMDSFHDAMYHSAIASRLARST</sequence>
</entry>
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    <accession>Q8H1V4</accession>
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        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="5" type="primary">hir3</name>
        <name evidence="7" type="synonym">LOC123397549</name>
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    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2003" name="Theor. Appl. Genet." volume="107" first="1094" last="1101">
            <title>Barley putative hypersensitive induced reaction genes: genetic mapping, sequence analyses and differential expression in disease lesion mimic mutants.</title>
            <authorList>
                <person name="Rostoks N."/>
                <person name="Schmierer D."/>
                <person name="Kudrna D."/>
                <person name="Kleinhofs A."/>
            </authorList>
            <dbReference type="PubMed" id="12928776"/>
            <dbReference type="DOI" id="10.1007/s00122-003-1351-8"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Positive regulator of hypersensitive response (HR)-like cell death. May be involved in potassium ion channel regulation.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="AAN17464.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK353827">
        <property type="protein sequence ID" value="BAJ85046.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8H1V4"/>
    <dbReference type="SMR" id="Q8H1V4"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0398400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0398400.1"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480710"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2620">
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        <property type="component" value="Chromosome 5H"/>
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    </dbReference>
    <dbReference type="CDD" id="cd03407">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hypersensitive-induced response protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Band7/mec-2_domain"/>
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    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
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    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
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<entry dataset="TrEMBL" created="2013-10-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>S4Z0K8</accession>
    <name>S4Z0K8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic</fullName>
            <ecNumber evidence="12">7.1.1.-</ecNumber>
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        <alternativeName>
            <fullName evidence="12">NADH-plastoquinone oxidoreductase subunit 4L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 13 16" type="primary">ndhE</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <geneLocation type="chloroplast" evidence="13"/>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2014" name="PLoS ONE" volume="9" first="e85761" last="E85761">
            <title>Sequencing of chloroplast genomes from wheat, barley, rye and their relatives provides a detailed insight into the evolution of the Triticeae tribe.</title>
            <authorList>
                <person name="Middleton C.P."/>
                <person name="Senerchia N."/>
                <person name="Stein N."/>
                <person name="Akhunov E.D."/>
                <person name="Keller B."/>
                <person name="Wicker T."/>
                <person name="Kilian B."/>
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            <dbReference type="PubMed" id="24614886"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="14" key="3">
        <citation type="journal article" date="2015" name="Mitochondrial DNA" volume="1-2" first="0" last="0">
            <title>The complete chloroplast genome of Tibetan hulless barley.</title>
            <authorList>
                <person name="Zeng Q.X."/>
                <person name="Yuan J.H."/>
                <person name="Wang L.Y."/>
                <person name="Xu J.Q."/>
                <person name="Nyima T."/>
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            <dbReference type="PubMed" id="26713356"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="15" key="4">
        <citation type="submission" date="2019-07" db="EMBL/GenBank/DDBJ databases">
            <title>Organelle genomes phylogeny of wild and cultivated barley forms.</title>
            <authorList>
                <person name="Makarevich A."/>
                <person name="Siniauskaya M."/>
                <person name="Pankratov V."/>
                <person name="Liaudanski A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="16" key="5">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
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    <reference evidence="16" key="6">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
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    <comment type="function">
        <text evidence="12">NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
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            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Plastid</location>
            <location evidence="12">Chloroplast thylakoid membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the complex I subunit 4L family.</text>
    </comment>
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0111480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="267" mass="29387" checksum="4B83CC9E2C9FA434" modified="2017-11-22" version="1">MRPNRAASSGYWKATGTDKPIHDAATSQGVGVKKALVFYKGRPPKGTKTAWIMQEYRLAADPLAAAVNTYKPIKFRNVSMRLDDWVLCRIYNKTGLGSPMVPLLADYDHMADHDDLFGGSDFDDATCRPMIKSQQQQHQQQHQQPHDGRFPTFPSMSELFDDYAFVQMFNTDVEHGVHPSRNQLLPVGDSRHVVEPSYYAPSSSPDASGGSAGKRKAGSPEESATHQPSAKKLNGSCFDAPSQSANDWQAVASVLGGLNHYSMLPQF</sequence>
</entry>
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    <accession>A0A287IJ38</accession>
    <name>A0A287IJ38_HORVV</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123426807</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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    <reference evidence="7" key="1">
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045110702.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A287IJ38"/>
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    </dbReference>
    <dbReference type="GeneID" id="123426807"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0140850.1"/>
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    </dbReference>
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    <dbReference type="KEGG" id="hvg:123426807"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="202" mass="23454" checksum="103309C1732251B8" modified="2017-11-22" version="1">MARGKVQMRRIENPVHRQVTFCKRRMGLLKKAKELSVLCDADIGVMVFSPHGKVYELATNGNMQGLIERYKGSNTEAHGESGEQNKPQVIQQEVLLLRQEIDLLQKGLRYMYGENDTNHMNLNELQALESNLEIWVHNIRYTKMQIISREIEMLKTKEGVLKAANDILQERIIEQSGFLDTSSNMMMPQLPFQRTMESDYYF</sequence>
</entry>
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    <accession>A0A287N5B8</accession>
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        <submittedName>
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        </submittedName>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045124246.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="112509.A0A287N5B8"/>
    <dbReference type="PaxDb" id="4513-MLOC_37043.2"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338850.2"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338850.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338850"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="47"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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            <end position="165"/>
        </location>
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        <location>
            <begin position="168"/>
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        </location>
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        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
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        <location>
            <begin position="309"/>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338850.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="46650" checksum="54650EDCAF3D7805" modified="2017-11-22" version="1">MLRQSSSRNHRSKGLKLKKALQISLLVLVSVWLLYQVKHSYEKKAAYNENDANREAGDEHKDDQSQTGVIKLGRKDLPVKEADSSTLDERIEDEENEEMDPEMKRDEHDDDPIDEPDLDKDDDLPEPGEHSANKDEGSDGRAVFEDEERKERSQEDQEKSFHGDDVSSAVTHDPPSSQQDELTHHAQEKILFVDDASTAVPHENQEPEHKEEEDRKASEKGSRGENVSSSVDHDAQVTNPLPDEQLKNMDRIFEGTTNLSNGITFRGPGVNGTNVVGDHEASSKTNASSDPNTSTPSMVLESKPDLTPVNMTSNHAEPVTSNSTSLKGQHEQPVNSTAALDNQTKPFTDLTSAELNSPPNGTTVASGSADAQKATTRDGDSSGNNTDTFSSLVNNKEEAGDTQKEDADVSTKIMNKAIGEGEVLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W4J2</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <lineage>
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            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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            <strain evidence="1">subsp. vulgare</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
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            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0025440.1"/>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        <property type="gene ID" value="HORVU.MOREX.r2.2HG0136910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0166090.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0166090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430253"/>
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    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.90.280.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF13">
        <property type="entry name" value="PROTEIN TERMINAL FLOWER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0166090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19596" checksum="2A4C207482748A83" modified="2022-05-25" version="1">MARVLEPLVVGKVIGEVIDNFNPTVKMTVTYSSNKQVFNGHEFFPSAVVSKPRIEVQGGDMRSFFTLVMTDPDVPGPSDPYLREHLHWIVSDIPGTTDASFGREVVSYESPKPNIGIHRFTFVLFQQKKRQAMNAPSTRDYFNTRRFADENDLGLPVAAVYFNAQRETAARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WFP7</accession>
    <name>A0A8I6WFP7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFP7"/>
    <dbReference type="SMR" id="A0A8I6WFP7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0096840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol_A_exo1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="43"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25143" checksum="D60BE814B841E771" modified="2022-05-25" version="1">MPSRFLKVLAHGTTKLDVVYTNESREVPFSLEQLKEKWFDDAMEHEKFLGLDLEYTADQRGVAVIQVCFARHVLIFQWARSDKHCPELKDFLRSGITFATVDIRNDKLKMRHSFGIEIPVGCLVDLQMIFRLRHDRTLMAHMAVALIDEEYGDMKTSFPKSQNKIWEKDPLDPINIEYAAKDAYVSYELYRKIRVVNYGQRHLEVGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIU3</accession>
    <name>A0A8I6WIU3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIU3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0084110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0084110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0084110"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0069120.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0069120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0084110.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.1HG0084110"/>
    </dbReference>
    <dbReference type="OMA" id="FWRVRCA"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0084110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="73" mass="8340" checksum="43A3F52D9779D76C" modified="2022-05-25" version="1">MAFLGGSLRKMMEGKKSHDGSGLWRQAPATVRQLFWRVRCAMVRPKRPAVSFGYDLKSYSQNFDDGLVPVRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJS5</accession>
    <name>A0A8I6WJS5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJS5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0097860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097860.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097860.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26495" checksum="50AFAC93F7BF604F" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICIVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYVSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQ13</accession>
    <name>A0A8I6WQ13_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123443007</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 15">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 15"/>
    <dbReference type="RefSeq" id="XP_044975150.1">
        <property type="nucleotide sequence ID" value="XM_045119215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ13"/>
    <dbReference type="SMR" id="A0A8I6WQ13"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0240180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0240180"/>
    </dbReference>
    <dbReference type="GeneID" id="123443007"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0240180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0240180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0240180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443007"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14294">
        <property type="entry name" value="UBA1_UBP5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000371">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000099">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF10">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="18">
        <location>
            <begin position="158"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="17">
        <location>
            <begin position="310"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="16">
        <location>
            <begin position="607"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="16">
        <location>
            <begin position="668"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="755"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0240180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="795" mass="88647" checksum="B77211FB90C4C651" modified="2022-05-25" version="1">MDLLRANLHKVRIPEPTNRIHKDECCVSFDTPRSEGGLYVDMNSFLGFGREHVAWNFEKSENPVYLHIVQRRKPEPDETDRPLKKPTLLAIGVEGGFGEQEPEYDVTYEIVILPEFESLPFPSVDLPEKVRLAVDKVILAESADRKQQLASWVADKKIISAHAMDLQQLDNGVIVPPTGWKCSKCDKTENLWLNLTDGMILCGRWVWDGTGGNNHAVEHYQQTKYPLAVKLGTITADLEGADVYSYPEDDSVEDPILAQHLSHFGIDFSSLQKTEMTTAERELDHNTNFDWNRIQESGKDAEPLYGPGYTGLVNLGNSCYMASVMQVMFSTHPFISRYFEKQSLKAAFAISQADPTLDLNMQMTKLAHGMLSGKYSAPSPEGQEGIRPRMFKSVIAASHPEFSSMRQQDALEFYHHLIDKVDQANPGNHELNPCTGFKFIIEERVQCPSGKVSYNQHCDNFLTLSIPLHEATNKEQLEVFHEKKAAMDLDGKELSKEEIVRPRVPLEACLASFSGGEEVPEFYSTALNSKTTAIKTTGLKTFPDYLVLQMRKFVMGAGWVPKKLDVYIDVPDIIDISHMRSHGVQPGEELLPEGASCGNKAESARPVADEDIVSQLANMGFNYFACQKAAINTSNAGLEEAMNWLLLHTEDPDINDPISQDPMPAEDTIDEASLQTLVSFGFPEDVSRMALKASGGNIERATEWVFSHPEASNSVSADSSTSTVKDDNSHISDGSGRYKLTAFVSHMGTSTHCGHYVAHILKDGRWTIFNDNKVAASVDLPKDMGYLYFFQRISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSE0</accession>
    <name>A0A8I6WSE0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123440056</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
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    <dbReference type="RefSeq" id="XP_044972584.1">
        <property type="nucleotide sequence ID" value="XM_045116649.1"/>
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    <dbReference type="SMR" id="A0A8I6WSE0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277190"/>
    </dbReference>
    <dbReference type="GeneID" id="123440056"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0229630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0229630.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0229630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440056"/>
    <dbReference type="OMA" id="CQANGKT"/>
    <dbReference type="OrthoDB" id="1873160at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF22">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035295154" description="chitinase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="15">
        <location>
            <begin position="26"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="299" mass="31069" checksum="8F46D07937C7500D" modified="2022-05-25" version="1">MACKLKRSPLLPILLLAGMVGISHAGNIAVYWGQNGNEGTLADACTSGLYAYVMVSFLSTFGSGQTPAINLAGHCDPASGNCNVFSSDITTCQSNGVKVLLSLGGGAGSYGLSSTEDAQSVATYLWDNFLGGSSASRPLGDAVLDGIDFDIETGNSAHYDELATFLSQYSAQGRKVYLTAAPQCPYPDASLAPALQTGLFDNVWVQFYNNPPCQYASGDASNLQSAWNTWTSSVKVSGGFYLGVPASTAAAGSGYVSPGDLTSAVLPGVTGASNYGGIMVWDRYNDVQNSYSEQVKDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WU81</accession>
    <name>A0A8I6WU81_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123412440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961326.1">
        <property type="nucleotide sequence ID" value="XM_045105391.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS05G0353600 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0062160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="232" mass="24701" checksum="06489B6D89D1333E" modified="2022-05-25" version="1">MWTSWTRRKPAARRLTNSPSSNSSVTSSSKDHSGISSVLDDAAAPPAGGGKVVLHRVRSSTKLRTCKSFAAAEEAAAAPVAAERRVVLYFTSLRAVRPTFEACRDVRAILRGLRVGVDERDVSMDAAFLTELRVLMRRDRPPLPQLFVGGRLVGDADDVRALHESGELRRVVAGAPQLPPTPCASCGGSRFGPCVACGGSHRWFSEKTGGFRVCSACNENGLVRCTACCSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUQ7</accession>
    <name>A0A8I6WUQ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA excision repair protein ERCC-8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123444848</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044977641.1">
        <property type="nucleotide sequence ID" value="XM_045121706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUQ7"/>
    <dbReference type="SMR" id="A0A8I6WUQ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300880"/>
    </dbReference>
    <dbReference type="GeneID" id="123444848"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0251000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0251000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0251000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0300880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444848"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001356">
        <property type="entry name" value="Excision repair cross-complementation group 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001188">
        <property type="entry name" value="Transducin/WD40 repeat-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="99"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="392"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="50634" checksum="612E0400D07CAD5D" modified="2022-05-25" version="1">MWWEEVRRRERGELPARRFQALARSRRAASLALSNRKEIATPHLGAVNSLQVDLTEGRYLLSGASDGSAAVFDLKDATEYEAGFIAKHRSILLVDRQHEHGHKFTVSKAIWYPVDTGLFVTASFDNYVKVWDTNSTQVVMDFKMPGKVYSAAMSPIATTHMLIATGSADVQVRLCDIASGAFTHTLSGHHDGIMSLEWSTSSEWILISGGFDGAIRFWDIRRAGCFLVLDQLRSQLGRRPPVLDTTMDNDQKKLGRSSSSKSYSVQQRTANRKKQSKALHKSLTMVRGHTQQKMHPGMSSSQNHKTAHYGAVTGLRTTTDGMHLLSSGSDSRLRLWDIDSGCNTLVNFEAMRLQTSKPLQLAVTDDPSLVFIPCMSSIKAYNTWSGTTFQTFRGHYDHVNCCYYNSQDQELYTGSNDRQILVWSPSTPALTEMEDDDKRQEGFAADEDNWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1T1</accession>
    <name>A0A8I6X1T1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X1T1"/>
    <dbReference type="SMR" id="A0A8I6X1T1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0208050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0208050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0208050"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000402">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1045">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="95"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0208050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="410" mass="45937" checksum="A1F5BE77156ED1DC" modified="2022-05-25" version="1">MVLMEVNSEFLIGRIYAVHKKTGPIKIVLPIISCLLLLACIVFSWMYIYRGKRAKKKKQKKLMLGYISTSNYLQSKNTDFPCFSYEDILSGTKFFADSNLLGRGGFGKVYKGALEGGNEVAVKRLSKSSGQGIVEFKNEVVLIAKLQHKNLVRLLGCCIHEDEKLLIYEYLANKSLDAFLFDHARKHVLDWLTRFKIIKGIARGLLYLHQDSRLTIIHRDLKASNILLDMEMTPKISDFGMARIFAANQNHANTTRVVGTYGYMSPEYAMGGTFSVKSDTYSFGVLLLEIVSGLKISSAQLKMEFCSLISYAWRLWKAGKATELVDSFIAASCPLHEVVRCIHVGLLCVQDHPNDRPLMSSVMFMLENESAMLPPPKHPVYFALGNPEGEQASESTSSSNAMSITMLKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2E6</accession>
    <name>A0A8I6X2E6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2E6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0300490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0300490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0300490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="40"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0300490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="165" mass="18030" checksum="12EB3B2947EC42BD" modified="2022-05-25" version="1">MSSFSVGSCSGAGIRRRCAAARSPVSYREQPMDYEPTVFCSRCGRKAPRWISWSVPNPGRRYYACVESKHGFLQWHDEPTYPFLRVLLGDLRDRVWSLEDSVAALCGDGDPAAREVQERTAGAGAVQEAVHERTAGVGVVGDSVRLMVCGIVIFVSGLFVGMIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X508</accession>
    <name>A0A8I6X508_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444887</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044977700.1">
        <property type="nucleotide sequence ID" value="XM_045121765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X508"/>
    <dbReference type="SMR" id="A0A8I6X508"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0301880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0301880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0301880"/>
    </dbReference>
    <dbReference type="GeneID" id="123444887"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0251820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0251820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0251820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0301880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0301880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0301880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444887"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000060">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000344">
        <property type="entry name" value="Transcription factor MYB44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="150"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="150"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="202"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="202"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0301880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="458" mass="49041" checksum="A291AC85AA34CDCC" modified="2022-05-25" version="1">MSSGTAVSPQPRSSSPPTAAVSAHSRTAPAADEPEAPDPAQPPPHHASQSPPPSVSDEAEAPAHPQPLPRHPLPSSSSSSPSTAADETQGPVQPPPQTQMHRPSSPSPSTAEPQTPVLPQPNHSSPPLSGDDDDDDVVITGAVGGASTAADERVKGPWSPEEDAMLTNLVMRDGARNWTTIARGIPGRSGKSCRLRWCNQLDPQVKRKPFTEEEDQMIISAHAIHGNKWASIAKLLVGRTDNAIKNHWNSTLRRRYCTGTRCTRSASAEQPIREMSRAASEERWPLSGPSSFSVMEVKEAPVQTVSESSARALQIRDINNFSSEAVERSYPVRPVATVGAFRPYSLGSAQPMQMEMSSSTKFPTLQPFTPEIAVSKFADTTCFATDVPNKCGHGCCSTEERPRNNSLLGPEFKEFIDHPPILSSSFASLVSEISSIAWMNSGMQSSNSGNLVQSNPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X855</accession>
    <name>A0A8I6X855_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123445576</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X855"/>
    <dbReference type="SMR" id="A0A8I6X855"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19125">
        <property type="entry name" value="AKR_AKR4C1-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000010">
        <property type="entry name" value="NADPH-dependent aldo-keto reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044498">
        <property type="entry name" value="AKR4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="71"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="39467" checksum="97C603BA001026EE" modified="2022-05-25" version="1">MSAAASASPVIARQVNRRTPRLGLYKLARVSGMGALGRLLPSARALASVGERQGMALHFVLNTGAKIPSVGLGTWQSEPGVVGDAVYAAVKAGYRHIDCAQVYGNEKEIGFALKKVFDEGVTTRKDLFITSKLWCTNHAPEDVPVALDGTLQDLQTDYVDLYLVHWPIRMKKGAGFNPENVIPADIPATWAAMESLYDSGKARAIGVSNFSCKKVEELLAVARVTPAANQVECHPVWQQAKLRDLCASKGIHFSAYSPLGSPGTFKAFSVLEHPVVVSTAEKLGKTPAQVALRWGVQAGHSVLPKSTNEERIRANIDVYDWSIPGDLFAGFSEIEQAKLIRGKFWTHPEGVFKSEEEIWDGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDV6</accession>
    <name>A0A8I6XDV6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDV6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0263250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0263250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0263250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0218430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0218430.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0218430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0263250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0263250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0263250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF31">
        <property type="entry name" value="OS06G0482850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0263250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="113" mass="12382" checksum="A06D882AB5D0AF7F" modified="2022-05-25" version="1">MVASITGNRLPVNSDEVAKLLLDSFDRADGDFSIHRHLPEDFLILFSSLTTKARLAGDHFIKSPRFSLSIASGVQIGPRQRRRVGVPCGARAAWSPCQAWHLSTAEHILGQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEQ7</accession>
    <name>A0A8I6XEQ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>oxalate + O2 + 2 H(+) = H2O2 + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:21880"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
            <dbReference type="EC" id="1.2.3.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Secreted</location>
            <location evidence="2 16">Extracellular space</location>
            <location evidence="2 16">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the germin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEQ7"/>
    <dbReference type="SMR" id="A0A8I6XEQ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0279630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0279630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0279630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0335360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0335360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050162">
        <property type="term" value="F:oxalate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 13 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035339365" description="Germin-like protein" evidence="16">
        <location>
            <begin position="24"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="17">
        <location>
            <begin position="63"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="228" mass="23919" checksum="7404B739EA3B69B3" modified="2022-05-25" version="1">MGYSKTLAVSLFAVLLLAPAVLASDPDPLQDFCVADLDGKAVSVNGHPCKPMSEAGDDFLFSSKLAKAGNTSTPNGSAVTELDVAEWPGTNTLGVSMNRVDFAPGGTNPPHVHPRATEIGIVMKGELLVGILGSLDSGNKLYSRVVRAGETFLIPRGLMHFQFNVGKTEASMVVSFNSQNPGIVFVPLTLFGSNPPIPTPVLTKALRVEAGVVELLKSKFAAGTNTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHR4</accession>
    <name>A0A8I6XHR4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123442389</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044974354.1">
        <property type="nucleotide sequence ID" value="XM_045118419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHR4"/>
    <dbReference type="SMR" id="A0A8I6XHR4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0220260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0220260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0220260"/>
    </dbReference>
    <dbReference type="GeneID" id="123442389"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0183450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0183450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0183450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0220260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0220260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0220260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442389"/>
    <dbReference type="OrthoDB" id="749268at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="354"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0220260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="653" mass="72546" checksum="F64D0E6A4325AD12" modified="2022-05-25" version="1">MISTLQEAQFVGLASSHCLQSSFFSQKMFKALVVALLFSVLNHGSNRATASGDGDFFHNCPPFRCGDGRTEIRFPFRLATSPPSCGAPGLELSCSREADTILLHPILGQCKVTGIDYSAGEMNVIPLEESWTRCPLQKISITNLATSVYKPLRHGGEPIILVRCSRELIPKEKTSIRGFRREDSIVGPISCMSNTSKFTYLMAGMEPMSLLPLGCTVVSNGMSTPQDFDGNPVELFAEIAKRVIASGETTLTWSVPNITDICLDCERNGHPCGFSTQRGQAFCKPQGSRVKVIAATSSVATFVILLLTAATALYRSLKSKVDEEVRMKIEMFLKAYGTSKPTRYTFSEVKKVTRRFKDRLGQGGFGSVYKGQLANGVPVAVKMLENSRSDGEEFMNEVATIGRIHHANVVRLLGFCSDGTRRALIYEFMPNGSLEKYIFADESDICRELLAPNKMLEIASGIARGIEYLHQGCNQRILHFDIKPHNILLDYRFSPKISDFGLAKLCTRDHSIVALTAARGTMGYIAPELYSRNFGRISSKSDVYSFGMLVLEMVSGRRNSDPWIENQNEVYMPEWIYEKISTEQELESTRDMTREEKETIKKLAIVALWCIQWNPKNRPSMPKVLNMLTGTLQGLTMPPKPFVSSPGHPVPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIQ4</accession>
    <name>A0A8I6XIQ4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123449914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peroxisomal proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxin-11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983216.1">
        <property type="nucleotide sequence ID" value="XM_045127281.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983217.1">
        <property type="nucleotide sequence ID" value="XM_045127282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIQ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413650"/>
    </dbReference>
    <dbReference type="GeneID" id="123449914"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449914"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044375">
        <property type="term" value="P:regulation of peroxisome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF44">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="26149" checksum="7151331C1528949B" modified="2022-05-25" version="1">MGSLEATRVELGLAVLYLNKAEARDKICRAIQYGSKFISNGQPGTAQNVDKSTTLARKVFRLLKWVNDLHALISPAAKGTPLTLVLLGKSKNALLSTFLFLDQFVWLGRSGIYQNKERTDRIARISLYCWMASSVCASLVELGELKRLSKAMRKRAKELRGADKYEDEQYLGKMKQSDDRLLALVKAGMDVVVAVGLLQLAPKKVTPRVTGAFGFVTSLISCYQQLPARAPLAKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIY4</accession>
    <name>A0A8I6XIY4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123450622</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044983715.1">
        <property type="nucleotide sequence ID" value="XM_045127780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIY4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0387000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0387000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0387000"/>
    </dbReference>
    <dbReference type="GeneID" id="123450622"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0321900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0321900.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0321900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0387000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0387000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0387000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450622"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374:SF471">
        <property type="entry name" value="OS03G0279700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0387000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="20421" checksum="FCC38E7707A4CF3E" modified="2022-05-25" version="1">MAHGKRSRQQQAETTSVSLMDLDGGDMARILMLFSGHHNSHGHHGFAAPASPERVFECKTCNRRFPSFQALGGHRASHKKPRLADGGEPPKPKVHGCSICGLEFAVGQALGGHMRRHRAVAAAGPGVERGLSLELDLGPRDDGKKKTTAVAAGLMIDLNVPALALEEEPADRAMLGLAVEMQLPVVVDFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJR7</accession>
    <name>A0A8I6XJR7_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123440988</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973458.1">
        <property type="nucleotide sequence ID" value="XM_045117523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJR7"/>
    <dbReference type="SMR" id="A0A8I6XJR7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0322090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0322090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0322090"/>
    </dbReference>
    <dbReference type="GeneID" id="123440988"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0322090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0322090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0322090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440988"/>
    <dbReference type="OrthoDB" id="1893065at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF186">
        <property type="entry name" value="OS09G0364100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="62"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0322090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="41352" checksum="0815DADD0F7C0606" modified="2022-05-25" version="1">MTSSSAASSSHLHITITNASSTITTNTKSQSQHNHSSSVSPRSGGSGGGGSGSGSGSGTTNQACAACKYQRRKCNPDCPLAPYFPADQQRRFLNAHRLFGVSNILKTLRRLKPELCEAAMQTLIYQAEMRAMDPVGGCCRMIIDLEHTSELLAAELAALNQHLDLYRQAASGVAGGDVMDGPCADLEVTSSNHQQEQLLLHADQDQVVDTLYVAQEVADPVIQNGADHDDNRQPQYHGGQQQQQLYDYFYYEATGAGGDEAGRKPGGSGVDINVDVMQHFDYDSSCEVDDHHKVDQLEPMISSSLDEHYPIGQKEYEMKVASFVDVLDVRPEMQAMDGNADIGVKEELQEEEEEDPKNNIELSKATHMAESSHCRLGLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKL8</accession>
    <name>A0A8I6XKL8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKL8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0434220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0434220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0434220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0434220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0434220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0434220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0434220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25927" checksum="065AE3487DB250D6" modified="2022-05-25" version="1">MSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEEPIHWSYGLGVTFDMAVQDAAYSMLTIMRARHALLQNSVFCYVPASQPGEEAYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWMAFTQLAPVVQTGYGDMDMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELLGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMB8</accession>
    <name>A0A8I6XMB8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF1">
        <property type="entry name" value="WISKOTT-ALDRICH SYNDROME PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1299"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1667"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1727"/>
            <end position="1956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1324"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1678"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1734"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1784"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1816"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1835"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1862"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1879"/>
            <end position="1889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1937"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1947"/>
            <end position="1956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2149" mass="234625" checksum="84A0D051DE6AB7E4" modified="2022-05-25" version="1">MIRYQVRNEYGLADPALYAPEEEEDDPEALLEGVAMAGLVGLLRQLGDLAEFAAEIFHDLHEDVMATASRGHGLMLRLQQLEAEFPAVEKAIIAQTDHSNYLHDDGVEWHSNLQLNQNLITIGDMPRFILDSYEECRGPPHLFTLDKFDVAGAGASLKRYSDPSFFKMGQTSNMIEPDYLREKKPRRIKKKAMRRKGETLESLLIANSESQITSSKDRSSRKVPPRTTKLKSRHLRDSYNKTISRICREQLQEVISSQQKILSNYSARNFHAKFRSTDSSEIISSFGELDNFSASAQYFTKHELTKVVPVDESDTLVTASAPINGQDFLEVDDGQFLATQQEHFEMEQICKGSPLQSVQEEKLQLAVDPVYHDDDPCRPDNIGSDQDKFIDTLNTESEGEADHSMKSQKNLSAKMEVDNLNCDGKEAESVLDVQFPELGPAEVLSSGLSNPCNDTEPTRADSFLLSDSSPSAVSDTKDTDSDSDSCKQLSGDNWINDKESCNDVDLMDVSSSSSVTSDENGNFETETNLNGSVQNQEVSILPSNDHAVAIPSSEKQLSQTTSGLDVIVLPGLASISSDYHEIAYRSAEGGQNKVDDTSTISGQPNDVSHAGGKFEVPHADDSLLHSRIVNQEQLQLPKKQFEECTSLGTAVQETDSKFASLPDMDPVVHMNDLEFNNVAHPAEIYTSTTPTSLYPDGSHKHLDKLDSGVIPIHSSTPDNQLYESDHGKLVEELHCLPDEDLYKHVAEDQEMVVLEKGPCSVWLDAHREDSMQASVVPMHFSDVQVIPECTESVPTSQHDTAAHPSEMLEPSPQVLNDDIKPSLIDAPPASSTAPILGTSMSCMEYHESIETGKNVECGEVLVDAELVEESTTSRFEDDMIPSEEEHGDGAKYTEKSEVLPTNYREENSSHDVPLQSSPFREDIEAGEATCENLGSLEESRGHIYRESMLQTANLPQPIEIGTSGKTLGGSDDILYVPHSHFPEESFCQEELPDETTLIAEEVPCQCNLDKDVTVSSNSNMVGEQLSDVDQDLVRVLSAQDSFGTNPFVDPGYIVSSTDPLPSMSYQPCCSEEEDDFLSELLIQQVNIGAKENLYLLDDSLWEPATPPDEAPLPSEVMTEQDFRSLCHEYHEIDFTAATEGFDCKPSSDSKDITAECSQPELDQVAVCSQPDSQLAECSSAMDIIGETFVPISAMEVPDGGKQAVDSDLRSHESLGDVRNPELDIPSVPVKLEQEQHPLPEVVSDSGIYGSRSGNIIPVKEEQDPGANLLPRALINEKVGELDVHSSNSPVEPVVGARGLDELDGLPSRKPIATQESDGCVLDEPNSQSVPPSSTDKTVDNIDVPPLRIALAAEESENRFTGENVSQIGTSSICEKIDESGAPPSSNVVLVEKEAQVCAPGELDSEIASWFLPNKKIDELDCSPLSGSVLVEDELEHHVLGVPDSQIAPYSSVNDKTVGPGAATSVNDTEVGWETCPSPELDPQFAPCPLSDYIVGKLDETPACNVQVEAENGPYYSPEFDTRISSYSSVNDKIDVPGAATSIHDMEVEEGWEACASPELDSQSALCPMSEDRVGELDGSSSCNVQVEAENASYCSPRIAPGSLNSSALAERSAMASTSVMHSTEENYRVSPVPPHTEPFPSVCSEDPPKLPPLPPLQWRLGRPRLGFLSTKDRVPDPASTTTSILPVSSQDMDNGLVSLDQMEEPIASVSSQDIQERYQNFMVDNSDQRVESGKPSTLQTVNDVTGEHDMPFSEACGNIKHEGHITSSPTEAEEHRNGSGATEHVIKQQDRQQHLLCSDISGTTEHLKSTDPAASEDDKTVDDHNGARNVHLHTASPSTAGHVSKNGSYRQSLHGESLSGTSDKEEHSSNASDEKNLKDQSIISGLPSDTTEHTASHSVLEEGNSQESKLQEKDADNLKGGPSEGQSHTAESMVSEDYPHGDHNLDTESILQPNPLWPSNISKYLDDLGEGGYAHAEQPPVTGWTVGPQMLHPNYGISSEGSRFEPEVTDYRLTRKPVSVRNIPRNPLVDAVAAHDRSTMRKVSELAPATDKPNTNDKNLWLEQIRNKVGELAPTADKPNTNDKNSWLEQIRNKTFDLKPVGSAQPTSMRAPARASSGNLRVAAIIEKANAIRQAVGSDDEDDDNWSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMI4</accession>
    <name>A0A8I6XMI4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949628.1">
        <property type="nucleotide sequence ID" value="XM_045093693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMI4"/>
    <dbReference type="SMR" id="A0A8I6XMI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527220"/>
    </dbReference>
    <dbReference type="GeneID" id="123399272"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0438500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0438500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0438500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0527220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0527220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0527220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399272"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF61">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="63"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="179" mass="20364" checksum="4877630C934D9B8E" modified="2022-05-25" version="1">MAMSAQVAAVAAPRLFLPPSRRLNPSPAPPLLRSGSSSLRWTRRASVRVRAGAGGGGERRRESPYEVLGVTPSASPAEIKRAYRRLALKYHPDVNKEANAHQKFVRIKHAYNTLMNSESRPKHAEEEEEFDWLAVIIEQMEAYHYQDSDDDLDFEWKPEGWEQVFVYSLLIAILIYVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNP4</accession>
    <name>A0A8I6XNP4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNP4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0284460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284460.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="29126" checksum="48C1610153BC8AAD" modified="2022-05-25" version="1">MPGRTSRQVVCLTRCIDVPGHTAMLVRVMSVCDYRWYPEYTVEEQYRDFNQSEYVCTVRVFPDYPRAEEPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFYYIPASQPGEEGYLSGVYFDSSMEDPLLQSTAKMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLDPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPRVELPGHGGNIYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQS1</accession>
    <name>A0A8I6XQS1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123444917</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable ATP-dependent zinc metallopeptidase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977738.1">
        <property type="nucleotide sequence ID" value="XM_045121803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQS1"/>
    <dbReference type="SMR" id="A0A8I6XQS1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302510"/>
    </dbReference>
    <dbReference type="GeneID" id="123444917"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0252370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0252370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0252370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444917"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000005">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF25">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="351"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="82"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="761"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="761"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="798" mass="86935" checksum="B3C32D42996544D8" modified="2022-05-25" version="1">MSLSALSRALARSSRSRQGYLLGGHGVFSAPLPSHSPPPGGDSAGLRLVRRYLTSALGSRAAAANGAGKARGWRSLLANSQSRRMFSDQSKKNFPKEKEVPKGDGSNKSESKKESSSGGQPKAQEKFVKLFEDTLGPVFLLGLLVLATVPGSSEKEISFQEFKNKLLEPGLVDHIVVINKTVAKVHVRSSPSTKQNQHSDASITTSHLPGQEAPSKSKYYFNIGSVDSFEEKLEEAQQVLGIDRHDYIPVTYADKISWFQEIMKFAPTLFIVGLLYVVGKKMSISIGGGSGQGGRSIFSIGKAQVTKMDKNSQNKVFFKDVAGCDEAKQEIMEFVHFLKNPDKYEALGAKIPKGALLCGPPGTGKTLLAKATAGESGVPFLSISGSDFLEMFVGVGPSRVRSLFQEARECAPSIVFIDEIDAIGRARGRGGFSGGNSERENTLNQLLVEMDGFGTTAGVVVLAGTNRPDILDKALLRPGRFDRQISIDRPDINGREQIFRIYLAKLKLDKEPSFYSQRLAALTPGFAGADIANICNEAALITARRDEKLITMQHFESAIDRVIGGLEKKNTVISKLERRTVAYHESGHAVAGWFLEHAEPLLKVTIVPRGTAALGFAQYVPSENLLMTKEQFFDRTCMTLGGRAAEEVLIGKISTGAQNDLEKVTRMTYEQVAVYGFSEKVGLLSFPQRDDGFGFDKPYGGQTASMIDTEVRELVAKAYNTTTELIRKHKDKVAQVAELLLEKEVLHQDDLVQVLGERPFKTDEPSNYDRFKQGFMDSDNGKSAEVDPSSSAGEAVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRY0</accession>
    <name>A0A8I6XRY0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the cohesin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0496680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21793">
        <property type="entry name" value="Rad21_Rec8_M_AtSYN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.580:FF:000002">
        <property type="entry name" value="Sister chromatid cohesion 1 protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF55">
        <property type="entry name" value="SISTER CHROMATID COHESION 1 PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="8">
        <location>
            <begin position="425"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="242"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="483" mass="52137" checksum="5B269AF267B7EDCC" modified="2022-05-25" version="1">MVTLDEDGSSSACRSLRFEPGPHEQETFPPFPEDTVSVDLPPPQDSLHTTGNGHQDPPEISRRATDSATTRCTDGGGSDTMDEDPRSPFMEKVTTPRAMSPSGRGSPLPGTSIPDVPIGVSHDHDHMEEDEKSGGTGILVPAAAFVLAPSPPPVQGNKRRRAQNPRIDEIGVLSNDDMRSQVHGDELQRLVRRRKPLPHTALDTWKFNKRREMDSLLPLVEGMCTDLHKAYEWNYPRVSDSDADVANTDAPAPPVNADTHEPEPHLTPVSSQEPEPHLTPISSENGEATPINTTPELPRFSLQMDLSTVREEDDSPFIKTPAGRTGTPGSGLGGTGATATATHGSYATPGQNTRESDPNGSQFPFGDDYLDEDLPEIPGLINTPSATPSADTGARGLSSMSTRTRAAAKYFKGMMMSSATSQDQQRGKFSLNIILDGRTRKQAASMFFETLALKSCDYIDVLQEEAYGDISVSVRPSLSSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUF7</accession>
    <name>A0A8I6XUF7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123447297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUF7"/>
    <dbReference type="SMR" id="A0A8I6XUF7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0341080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0341080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0341080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0409540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447296"/>
    <dbReference type="OrthoDB" id="406156at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000041">
        <property type="entry name" value="Hyoscyamine 6-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="184"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="37804" checksum="383AF4831040A3B5" modified="2022-05-25" version="1">MENILHATPAHVSLPESFVFASDKVPPATKAVVSLPIIDLSCGRDEVRRSILEAGKELGFFQVVNHGVSKQVMRDMEGMCEQFFHLPAVDKASLYSEERHKPNRLFSGATYDTGGEKYWRDCLRLACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCDGMGIRADYFEGDLSGGNVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPSAVNGLEVSYKGDWIKVDPAPNAFVVNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTTMFRDFMRIYNVVKLGSSLNLTTNLKNVQKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUG8</accession>
    <name>A0A8I6XUG8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976263.1">
        <property type="nucleotide sequence ID" value="XM_045120328.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976264.1">
        <property type="nucleotide sequence ID" value="XM_045120329.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976265.1">
        <property type="nucleotide sequence ID" value="XM_045120330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUG8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0268760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0268760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0268760"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0268760.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0268760.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0268760"/>
    </dbReference>
    <dbReference type="GeneID" id="123443799"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0222760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0222760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0222760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0268760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0268760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0268760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0268760.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0268760.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0268760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443799"/>
    <dbReference type="OrthoDB" id="1875894at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33401">
        <property type="entry name" value="LIGHT-HARVESTING COMPLEX-LIKE PROTEIN OHP2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LOW AFFINITY POTASSIUM TRANSPORT SYSTEM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0268760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="205" mass="22462" checksum="7E90A2D3232A6688" modified="2022-05-25" version="1">MLLAVEGGGFFSSSASGYSHGLALLLLGRKEEEKPVKVSPWNQYGLVDGEAEHVYHLASTKNQAPRKCAPFVCFGSTASELEGVYPAKLSSSNSLDSLEEPSRSTEKKVTANGSITGDERKGCLKSNSKRDSSEHSILASEGEEPRESLEEVQTLKSGMERRKVQWTDTCGKELFEIREFEASDGSLSDDELENEGFRKCECVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUQ1</accession>
    <name>A0A8I6XUQ1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger-XS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUQ1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0232610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0232610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005381">
        <property type="entry name" value="Znf-XS_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03470">
        <property type="entry name" value="zf-XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger-XS" evidence="2">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="20478" checksum="E59AA9170A3F8EDC" modified="2022-05-25" version="1">MEGACRAVAVADLPKQQEVDTTATVVAAAAEEKEVPVAKQEDMTATAIATAEVPKQEDTTLSGAADDEEKEATCMKRKLAKTGFKSPYDSDYDDDEPYEYNSGDDVEDGNKESFIEKEAQKIREKGKAAYFRRGMFRCPYCTTKPKPRDGVYEHLMSHAHGLLRSADQDIKTRAEHAALLKALGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUX8</accession>
    <name>A0A8I6XUX8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Generative cell specific-1/HAP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123397738</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAP2/GCS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948210.1">
        <property type="nucleotide sequence ID" value="XM_045092275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUX8"/>
    <dbReference type="SMR" id="A0A8I6XUX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497850"/>
    </dbReference>
    <dbReference type="GeneID" id="123397738"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0413040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0413040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0413040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397738"/>
    <dbReference type="OMA" id="YRYPLFY"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061936">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in double fertilization forming a zygote and endosperm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048235">
        <property type="term" value="P:pollen sperm cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010183">
        <property type="term" value="P:pollen tube guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040326">
        <property type="entry name" value="HAP2/GCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018928">
        <property type="entry name" value="HAP2/GCS1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31764:SF0">
        <property type="entry name" value="GENERATIVE CELL SPECIFIC-1_HAP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31764">
        <property type="entry name" value="PROTEIN HAPLESS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10699">
        <property type="entry name" value="HAP2-GCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0278">Fertilization</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035260346" description="Generative cell specific-1/HAP2 domain-containing protein" evidence="13">
        <location>
            <begin position="33"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Generative cell specific-1/HAP2" evidence="14">
        <location>
            <begin position="54"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="614"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="616"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="704"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="726" mass="80666" checksum="F7E77D6DC2055075" modified="2022-05-25" version="1">MAPRPPRPPSRPAHHAVFLLLAAAALVGPAVGVEILSKSRLERCALDSGAGGALACDRKVVLNLAVPSGSSGGESSLVAQVVEVEEDDTQAMQTVRDPPVITINKSATYALYDISYIRDVAYKPEELFTRTHKCESDAGANVVGACERLRDQNGHVIEHTEPVCCPCGNNRRISSSCGSIFDKMVKGKANTAHCVRFPGDWFHVFGIGKWSRGFSIRVQVKKGSSVTEVIVSPQNKTVVSKDNFLRVNLIGDFDGYTSIPSFEDFYLVTPRKGGGDGQPQVLGDEFSRWMLLERVRFTLDGLECNKIGVGYEAYRNQPNLCGAPLCSCLYNQLWNFKESDDNRVNRNQEPQYIVQGRFDRINQHPDAGVHTFSIGITEELNTNLLIEVSADDINYVYQRSPGKILSINVPTFEALSQVGTAKVTIKNIGKLEASYSLTFDCLSGITYVEEQFFIMKPDQVLVRSFYLRSSSDQASKYRCSAILKASDFSELDRAQCQFSTTATVLDNGTQIGPPNQHKKGGIRGFIEAIETLWRNTWDSVIDFFTGRSCSTKCSSFLDLSCHIQYICIGWLVMFGLLLATLPAVAVLLWLLHQKGLFDPLYDCWEDVFGPPEAAHGKHGGGRGHHAHTHAHHHQHHHHSKHPTAYAHKKRSSGAAGQQHHHHHHHVPHKHGGQPDGGERHRHAAALGVQHRDAGHKHHRHGKAAQRERERAQDRDREHRHHHSRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XV32</accession>
    <name>A0A8I6XV32_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        <location>
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            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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</entry>
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        <lineage>
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            <taxon>Triticodae</taxon>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">cv. Morex</strain>
        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0278430"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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            <end position="229"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0278430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <sequence length="232" mass="25611" checksum="FA265E5C1F5EA4F4" modified="2022-05-25" version="1">MSSDPGADDRLSLVMEYVGPSLHHVLHGARRGCPFQEDVVRYLMRQLLGGAKHMHERCGVVHRDIKPENVLVGDGVKICDFGLAMRTSTQSPPYGLHGTRSYMAPEILLGKPDYDAKVDAWSLGCVMAEILLGDRLFGRAADDADQLLRIFYILGVPDQISWPSYNSMPLAGELVAPPPTTSTPHRNRLREMFPEDCLSIQGFQVLSGLLSCDAHKRLSAADALELPWFAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWI5</accession>
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        <recommendedName>
            <fullName evidence="17">NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447499</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
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        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the complex I 75 kDa subunit family.</text>
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    <dbReference type="SMR" id="A0A8I6XWI5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337500.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:ATP synthesis coupled electron transport"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NADH dehydrogenase [ubiquinone] iron-sulfur protein 1 mitochondrial"/>
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    </dbReference>
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        <property type="entry name" value="NADH-quinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="COMPLEX1_75K_1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
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    <keyword evidence="5" id="KW-1278">Translocase</keyword>
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        <location>
            <begin position="71"/>
            <end position="149"/>
        </location>
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        <location>
            <begin position="149"/>
            <end position="188"/>
        </location>
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    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="286"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
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        </source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="744" mass="80213" checksum="7088FFC0072FC6F1" modified="2022-05-25" version="1">MAFLARALRHSKPYLPSRGPAAGASCRWISSTAAAGSPEAGAAVAPADPEMPPPREPVGGARVELPPNPEDALEVFVDGHAVRIPKGFSVLQACEVAGVDIPRFCYHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTNTPIAKKAREGVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGADRGRFTDMKRSVVDKNLGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGMLGRGSGEEIGTYVGKLMTSELSGNVIDICPVGALTSKPFAFKARNWEMKGTETIDVTDAVGSNIRVDSRGPEVMRIVPRLNEDINEEWISDKTRFCYDGLKRQRLNDPMIRGPDGRFKAVTWRDAIAVVAEVLNQVKPEEITGVAGKLSDAESMMALKDFVNKMGSDKVLCEGNGPNPPADIRSNYLMNTSIAGLEKADVFLLVGTQPRVEAAMVNARIQKTVRATQAKVGYIGPPADFNYDTQHLGTGPDTLVEIAEGRHPFCSILKSAKNPVIIAGAGLFEREDQGAVFSTIETVAKKFNVTRPDWNGLNVLLLHAAQAAALDLGLVANPTESVKSAKFLYLMGADDINLDNLPADAFVVYQGHHGDKAVYRANVILPSSAFSEKEGTYENTEGCTQWTIPAVPTVGDARDDWKIVRALSEVAGAPLPYDSVAAVRSRIGMVAPNLVRVDERETSVISAEVKPPVKQQVSPAPFKAAVENFYMTDAITRASKIMAQCSATLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXA1</accession>
    <name>A0A8I6XXA1_HORVV</name>
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        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123404078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0546120.1.CDS1"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.6HG0453960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0546120.1">
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        <property type="entry name" value="OS02G0564300 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="OS02G0564300 PROTEIN"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0546120.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="559" mass="61719" checksum="9E8D2C9E79F8A627" modified="2022-05-25" version="1">MPLLPPAAPPLPLSSHRLSLAFLSPPSPGRPLRRGDLVIRMGGGPRTFPGGVSKWQWKRMQARKAKQLLKARLARERQLYEMRKRAELRDAVVHLERPWDPDSAPVSAAALAPTLLSVAADDQLKGLADRFHRPGGVDLWNDRDGPRVFASPDTGLASARFFPRNAVHSVQPYARLGAGAGAEGAQGVRRNEAVEDEYGYSEPAVQLMERDGMWEPVIALEAGDGNNSSHRSWIHDDDANSDSDDDEEDAGFGHQRRAVVRRDGRRDGETVSAMRVGSERGRHRSGHGPFSDSEGASKGHLDQRWSDRNGGSRRKPPAARWKPWNAQGRNAIEKDRIGGGSLSESEVSRRGSEPKSRAGNREDAVDTVVKWKLSYDSHGNVVRKVRVGGEFDSNSDSGRDDKVEPKWRAQNRFSPSENRGGGAGLKYRPNANSGERPGRYTKGHNGDERGQFGNGFPSDLEEPTWNPRKKKEARNSNGSRDYNSDMKSKFRSGGSGAARRLDSTRPVMNANREDGGGRRPRGDGYSLRPTSELHRSVDRNGGRQFRGDGYSLRPTSELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXR1</accession>
    <name>A0A8I6XXR1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="primary">LOC123401962</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951760.1">
        <property type="nucleotide sequence ID" value="XM_045095825.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951761.1">
        <property type="nucleotide sequence ID" value="XM_045095826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXR1"/>
    <dbReference type="SMR" id="A0A8I6XXR1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549380"/>
    </dbReference>
    <dbReference type="GeneID" id="123401962"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0456520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0456520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0456520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0549380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401962"/>
    <dbReference type="OMA" id="HRALKMW"/>
    <dbReference type="OrthoDB" id="1933483at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000002">
        <property type="entry name" value="Gamma-glutamylcyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF19">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE 2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24220" checksum="90F60C7D9BFA3696" modified="2022-05-25" version="1">MVLWVFGYGSLIWNPGFDFDDKILGFIKGYKRTFNLACIDHRGTPQHPARTCTLESEDEAICWGIAYCVKGGLEKEREAMQYLERRECEYDQKISVDFYKDGNSLEPAVTDVLVFVSTPDPVGNKYYLGPAALEDMARQIATASGPNGYNRDYLFSMEKALSNISHEDDSIIVLADEVRKVLGRSTEKKVTGSDVPLKSHTPAVHISALPEGTVAVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZA5</accession>
    <name>A0A8I6XZA5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953109.1">
        <property type="nucleotide sequence ID" value="XM_045097174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZA5"/>
    <dbReference type="SMR" id="A0A8I6XZA5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0630410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0630410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0630410"/>
    </dbReference>
    <dbReference type="GeneID" id="123403224"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0523370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0523370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0523370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0630410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0630410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0630410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403224"/>
    <dbReference type="OrthoDB" id="1903788at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529">
        <property type="entry name" value="LOB DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529:SF64">
        <property type="entry name" value="OS02G0820500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="19"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0630410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="210" mass="21716" checksum="B4AE5533D834C755" modified="2022-05-25" version="1">MAAAPGGGGGGGVAGVAGSPCGACKFLRRRCVAECVFAPYFSSEQGAARFAAIHKVFGASNAAKLLAHLPLADRCEAVVTITYEAQSRLRDPVYGCVAQIFALQQQVAILQAQLMQARAQIACGVQSTTSPVSHHQQQPWSQDTSIAALLRQQENVSSFAAGGALLPELMSGDVSMLQQHCGGKVEGGGGGAGDLQYLAQAMMQSSNYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZB9</accession>
    <name>A0A8I6XZB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZB9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0559450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0559450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0559450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF3">
        <property type="entry name" value="OS09G0267800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="2">
        <location>
            <begin position="4"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0559450.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="358" mass="40010" checksum="F8896F8D6ABA16FE" modified="2022-05-25" version="1">MVADLTAIGQLFQLRYLKVSAASGSIELPTEIRGLVHLETLDIDCRTAQNIPSDIVMLRRLSHLIFPRDTGLPNGIGNMKSLRTLRCYCMGKTSLHDIVGLGELTNLRELTLTKTYKFDMVKAGVDALVDSIGKLCDLRFLYLACNLERYVDLLDSISDPPLCMENLRLGGWPFYKVPKWIGDLDCLHRLSLCVERLRTDDVHVLGQLPSLVYLSLKVLSIPQDSATIICTGSFPVLECLALMSRDDDATACMEFEAGAMPKLRRLVLGVHDRWGGGSAMPVGMVRLLSLEQIHVDNMSSVHFFFKKEAMPRPLHRVMHTAILLKKLKRSEVGTKSKSEGKINYKAMNAQGEYVDRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y121</accession>
    <name>A0A8I6Y121_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TMPIT-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123402921</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952823.1">
        <property type="nucleotide sequence ID" value="XM_045096888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y121"/>
    <dbReference type="SMR" id="A0A8I6Y121"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542310"/>
    </dbReference>
    <dbReference type="GeneID" id="123402921"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0542310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402921"/>
    <dbReference type="OrthoDB" id="2015098at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF4">
        <property type="entry name" value="OS02G0106800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="40724" checksum="F6798143C263AF98" modified="2022-05-25" version="1">MDGEGDGAQGLEEQAAAAAEAARELRDAAAALAARRAADEDALRRRAVALDADVRRLQGSVAPLDPSTLDKVEEELERARAAILDSDVAAFLPSKGNGKFLKKFVGPVNVRVARKEDKLKVKDEYNNYRDRAAYMFLLFPSTLLLLRWWVWDGCLPALAVQVYQAWLLFLYTSFALRENVLLVNGSDIRPWWIYHHYLAMLMALVSLTWEIKGQPDCSSKQRGVQLFLRWAIMQGIAMHLQNRYQRQRLRTRIALGKAKRMDVVAGETAGVEGQLLLLYPVLFVLQGFEGYVGVLLLQTAWHGLTSEWQVIVCGILLVVMAVGNFVNTVETLALKLRFKAKMKRTRHRQEPGQGGPSRLHQN</sequence>
</entry>
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    <accession>A0A8I6Y1N8</accession>
    <name>A0A8I6Y1N8_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123446597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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        </lineage>
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    <reference evidence="7" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
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        <property type="nucleotide sequence ID" value="XM_045123174.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0384590.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0384590"/>
    </dbReference>
    <dbReference type="GeneID" id="123446597"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.4HG0319870"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
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            <end position="576"/>
        </location>
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            <end position="92"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0384590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="576" mass="62968" checksum="A3FDF94C62F6D113" modified="2022-05-25" version="1">MGIPIRSLLVASLVLSSIALHVAAKTLDPYKVLGVDKNASQRDIKKAFHKLSLKYHPDKNKEKGAQQKFEEINNAHEILSDEEKRKNYDLYGDEKGNPGMGAGNFGSHDFTGGGPRTTYFSSGDGWQTMGGQGNSKTFSFSFGGNPGADGGNPFGFNLGDVFSNMFAGGSMGGGQHGGFAGSARPSARTSGQQKSPVTIQEVTMQTFNKEIADQGITWLLLFYTQQAKGQFVLESVLEDVARSLDGAVRAGKVNCDNEKTICKKSDVSVGKSARLFIYSYSTTEKGSLHEYSGDSDAKSLKTFCQEHLPRFSRRVDITQFNFPSNALPNLPEVLLLSTKKDTPAMWRAVSGMFRNRLVFYDAEVQDVSLPLLKRLGVKNVPALIGRTVNDEVQLLKDGISVKDLKSGIKELKTLLESFEKKNKKLASEQAKKPAKASEPKENKIPLLTASNFEETCGEKAPVCIIGVFKSNKAKEKLERILSEISQKTLIRGQSYSSGNAITYALLDGNKQSAFLSAFDKSGYKSSDKLLLAYKPRRGRFAVYKGEVSMEEAERFVASVLNGDVQFSATGQKPVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y492</accession>
    <name>A0A8I6Y492_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit domain-containing protein</fullName>
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    </protein>
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        <name evidence="4" type="primary">LOC123447128</name>
    </gene>
    <organism evidence="4 5">
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979638.1">
        <property type="nucleotide sequence ID" value="XM_045123703.1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334390"/>
    </dbReference>
    <dbReference type="GeneID" id="123447128"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0334390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
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    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1150"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1714"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2246"/>
            <end position="2290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2366"/>
            <end position="2393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2478"/>
            <end position="2504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2578"/>
            <end position="2618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1732"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1763"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2272"/>
            <end position="2290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2593"/>
            <end position="2603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="2618" mass="292934" checksum="B9C342CB7367F06A" modified="2022-05-25" version="1">MASSPVKFFSVFLAVSVVGWVVFTFAARLLAWFLSRVLRASVVFRVAGFNCLRDVTIKFSKGPLESVSIGEIKLSFRKSLVKLSFGVLSKDPKVQLLISDLEIVTRSSSRSKKTSKPSKPRSTGKGKWLITSSMARLLSVSVADLMIKVPDGAVDIKELKVDTFKIAGPNHILGVKLHILPLNVHLGDFGLIADPVGSGNQLDTFQSDQASLSSSEKFLAPFVCEDLLVICEFGHEKERGVKIVNLELKCGDVTANIDERLFYKKPAKPENNGGSENAGDAIAGTSTKQSSKSKSVLPALKKQMLAFPDKVSFSLPKLDVKFTHLGEGLSVDNNVMGIHFTSTKSLPQEDPEEATPHFDVQIVLSEIHLVREGSSSLLEVLKVAVVASLDIPLDPLLPIRAEIDAKLGGTQCNLMLSRLMPWMRLHSSRTKGMKLSKANSYQEISQTKEIKPIMWTCTVSAPEMTVMLYSPTGLVLYHACCQSSHVFANNIASKGIQIHTELGEMLVHMEDGYREFLKENIFGVDTYSGSLVHIARVSLDWGYREIEVQDMAETSRLALVFSIDISGIGVKFGFKHLESLLLNLMSFRDLFKNLSSSRKKDKEKDLEERRKKKTKGIEILKLSLQKFSITYSGDVNILNMPIADPKRVNYGTQGGQVIVDVSADGTKRTASITSEPPGIGRNLRFTSSLVISHLSVCIDKEKKSTQAELERVKAMYEEDHSSGVKVTLLDMQNAKIVRRSGGLTDVAVCSLFSATDINIRWEPDAHLALFETFIRFKWFLHHNKIQSSEKLMTETGSIKENEHVNIAAGAVKPQKSDKRGSIFAVDVEVLRISAELADGVEANMHVQSIFTENAKIGVLSEGLSLSLNGARVLNSTRIQVSCIPFSTANSLSAKLEPSPKRDWVIQGLDVHICMPFRLPLRAIEDAVEDMIRALKLVSAAKRSILFPDGKENSKKVKPGASSFGSVKFVLRKLTAEIEEEPIQGWLDEHYYLMRNKTCESGVRLKFLDDAISGTVDSNHCSSEGKFIYDGIEVDVHDTAALQRLREEIHKKAFRSYYVACQKKVFAEGSGACAEGFQAGFKPSSRRASLLSLSASELDITLTRINGGATEMVEFIKGVDPVCQEEDIPFSRLYGSDIALLAGSLVIQVRDYTSPLFSATSGKCQGRVVLAQQATCFQPQIHQDVYIGRWHKVKMLRSASGTTPAIKMYSNLPVYFQRGEISFGVGYEPSFADISYAFQVALRKVNLSSRDKNSGAANQPPKKERSLPWWDDIRYYIHGKIVLYFNETKWKILATTNPYEKVDRLQIESKYMEIQQTDGHVDISAKEFGMYISSLESMMKNCSLKVPPGVSRPFIYAPLFSLNVIIDWQCESGSPLNHYLHALPIEGEPRKKVYDPFRSTYLSLRWNFSLRPSQVQPDNGTSSPCYANNSMLCGSAFGSCSKIADVDFPTMNLGAHDLAWVFKWWSLNYSPPHKLRSFSRWPRYKIPRASRSGNLSMDKVLVEFFFRVDATPCCIRHATLTEDDPASGLTFKMSSLKYELCYSRGKQKYTFDCKREPLDLVYRGLDLYRPEVYLVRDVNLSSVENVSKLKTTIQSSQGKDKCTMGSFQEKHEDGFLLSSDYFTIRRQAPKADPARLMEWQDAGRNLEITYVRSEFENGSESDHSLSEHSDDDDGFNVVLADNCQRVFVYGLRLLWTIENRDAVWSWVGGISKAFEPPKPSPSRQYAQRKMIEQRQNADSSRLAQDATSSTHVGSPSMQHAEALGSTSPLHSKPNRSSDIAVKYGMFDDLDKGGNLHFMVNVVKPQFNLHSEDANGRFLLAAASGRVMARSFHSVVHVGKEMLEQALGTSSLHIPEPQPEMTWKKADLSVILKDVQAHVAPTDVDPGAGLQWLPRILGSSEKLKRTGALLERVFMPCQMYFRYTRHKGGTADLKVKPLKELRFNSPNITATMTSRQFQVMLDVLSNLLFARLPKPRKNSLQYPSDDEDVEEEADEVVPDGVEEVELAKINLEQRERERKLLLDDIRSLAGTGDSHTDHLSAEKDNSFWMINSGKASLVEGLKRDLLNLQKSRKFASSALRKALQKAAQLRLMEKEKNKTPSCAMRISMKISKVVWSMLADGNTFAEAEISDMVYDFDRDYKDIGVARFTTKYFVVRNCMASAKCDTLLSAWNAPPEKGVMLRVDAKQGAPKDGNSPLELFQVEIYPLKIYLPETMYRMMWDYFFPEEDDSQRRQDIWRVSTSTGSRRTRRVSSGADAVASTSYSVREHELPGRSGTSVNVSSWQGSHGDNPQVSKLQSLKANMVCGSHPELGRTSSFGKAWEESATENTTNNDVVSLLNSSNISSKNDGYAMAENTVAATEMFRSKTKDSKLVKSGRLSHEEKKTGKSQDEKRPRARKLMEFHNIKISQVELLVTYEGSRLAINDLRLLMDTFHRVEFTGTWRRLFSRVKKHIIWGVLKSVTGMQGKKFKAQNQRETLDGTVPENDLNLSDSDGSHHGKPDQFPVSWLKRPGDGAGDGFVTSIRGLFNSQRRRAKAFVVRAMRGDGDNEYHDEGSESDGEYPFARQLTITKAKKLLRRKFRPRGQKIIGPAMQDSLPSSPRETTPYQSDSSESSYEDFHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4K5</accession>
    <name>A0A8I6Y4K5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="8">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123451051</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044984001.1">
        <property type="nucleotide sequence ID" value="XM_045128066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4K5"/>
    <dbReference type="SMR" id="A0A8I6Y4K5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337540"/>
    </dbReference>
    <dbReference type="GeneID" id="123451051"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0281500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0281500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0281500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0337540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337540.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451051"/>
    <dbReference type="OMA" id="VCMELMD"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000350">
        <property type="entry name" value="Mitogen-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000813">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF829">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="56"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337540.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="337" mass="35636" checksum="5D3743E07BE27427" modified="2022-05-25" version="1">MAMIREKRLPQLHLALPVPSRAAAQDLAVIARRTNPAATKASTPSALSSQFRLADFDKLAVLGRGNGGTVYKARHRETCALYALKVQHYGDPAAAAEADVLSRTASPFVVRCHSVLPADASGDVALLLELVDGGSLDSIRSRRGAFAEAALAEVAAQALSGLAYLHARRIVHLDIKPANLLVSTAGDVKVADFGIAKVLARAGDQCTSYAGTSAYMSPERFDPEAHGGHYDPYAADVWSLGVTLLELFMGRYPLLPAGQQPTWPALMCAVCFGEPPVLPDGAASPELRGFIAACLQKDYRSRASVAELLAHPFVAGREVAASKRALRKLVADASSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7Z5</accession>
    <name>A0A8I6Y7Z5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7Z5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441740.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441740"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441740.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="29133" checksum="612CBB9CCCC17D81" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQRGEEGYLSGVYFDYSMEDPLLQSTIEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPIRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y957</accession>
    <name>A0A8I6Y957_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor GTPase-activating protein AGD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123407077</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956051.1">
        <property type="nucleotide sequence ID" value="XM_045100116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y957"/>
    <dbReference type="SMR" id="A0A8I6Y957"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0685200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0685200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0685200"/>
    </dbReference>
    <dbReference type="GeneID" id="123407077"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0568830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0568830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0568830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0685200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0685200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0685200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407077"/>
    <dbReference type="OMA" id="SMDEIQL"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07606">
        <property type="entry name" value="BAR_SFC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13250">
        <property type="entry name" value="PH_ACAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000019">
        <property type="entry name" value="ADP-ribosylation factor GTPase-activating protein AGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035670">
        <property type="entry name" value="AGD1/2/3/4_BAR_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF242">
        <property type="entry name" value="OS08G0537600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="292"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="11">
        <location>
            <begin position="490"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="730"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="763"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="439"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="686"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0685200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="823" mass="92732" checksum="7E3BC5405A6FD41D" modified="2022-05-25" version="1">MYFARLDDSPMFRTQIQSLEESAEVLRERCLKFHKGCRKYTEGLGEAYDGDIAFASALETFGGGHNDPISVAFGGPVMSKFTIALREIGTYKEVLRSQVEHMLNDRLLNFVDVDLHDVKDARKRFDKASLVYDQIREKYLSLKKGTRADITTAIEEELHSARSAFEQARFNLVTAISHIEAKKRFEFLEAVSATMDSHLRYFKQGYELLHQMEPYINQVLAYSQQSRERANKEQASLLERMHEYKKQIDRDSRLSGIGLSDSHNVDGIQTIGRSSHKMIEAVMQSSTKGKVQTIRQGYLSKRSSNLRGDWKRRFFVLDSRGMLYYYRKQINRPAGVCQVQRTNNTPEHGSGLLSRLFSSHYHGIHDEKSVARHTVNLLTSTIKVDAEQSDLRFCFRIISPMKIYTLQAESALDQMDWIEKITGVIASLLSAQSPEQCLHSPRSCGHDRSASESSSYTSSVELETSTSEDLTLEKNTGNGQHHHRTHIKPEKPIDLLRRVDGNTICADCGAAEPDWASLNLGALLCIECSGVHRNLGVHISKVRSLTLDVRVWEPSVINLFQSLGNMFVNRVWEETLPSSNNGSSIDNTSTNGSQAAQYFTTSKPKHSDPFSAKEKFIHAKYADKEFVRRHSIDEIHIAQQMWDSVTANDKKGVYSLIVASHANVNLVYGQMASGLFLNLGKALVQEQPASPPDGSPRFFDCNSHEKVSPRESLSPPSTSSHVDELDDRYEGFSLLHLACRVADVGMVELLLQYGASVNVSDSRGRMPLHHCILKGRHLHAKLLLSRGANTHATDRDGRSALQYAMDRSTSDEDMLALLEEHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAG1</accession>
    <name>A0A8I6YAG1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123449715</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044982964.1">
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</entry>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0568920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="13029" checksum="E4E1CEE5861C88BF" modified="2022-05-25" version="1">MARGVASVRSEGPFSIEFVSMDHDLKIAVSNVRGLNTRSRCNAIRSLLCTIGTSIVCLQETKMEFIYSHTVLDILGTEFDDSTYLPALGTRSGILLAWKSRVVTISDPLYTRSQSGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCJ6</accession>
    <name>A0A8I6YCJ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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    </feature>
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        <source>
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        <source>
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        </source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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            <strain evidence="8">subsp. vulgare</strain>
        </source>
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</entry>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
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        <text evidence="8">Interacts with OXI1.</text>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000182">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052101">
        <property type="entry name" value="Plant_StressResp_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983:SF20">
        <property type="entry name" value="OS03G0844100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983">
        <property type="entry name" value="PTO-INTERACTING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="68"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0532850.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="367" mass="40841" checksum="104A6D2EDF056AE5" modified="2022-05-25" version="1">MRNWLCCKGRRGEDDDEHEMEHSKAQGNKIDGKQKSSKPAEPEPDISPPTINVPELSFEDLKEKTDNFGSSSLIGEGSYGRVYHATMDDGRQAAIKKFDASENEHNDEFLKQVSLVSRLKHENLVEMLGYYVEGNYRILAYEFATMGSLHDVLHGRKGVQGAQPGPVLDWMQRVKIAIEAAKGIEYLHEKVQPSIIHRDIRSSNVLLFEDFKAKIADFNLLNQAPDMAARLHSTRVLGTFGYHAPEYAMTGQLTQKSDVYSFGVVLLELLTGRKPVDHTMPRGQQSLVTWATPRLSEDKVKQCVDPRLKGEYPPKGVAKLGAVAALCVQYEAEFRPNMSIVVKALSPLLQQRAAAPAASELAQAPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPR5</accession>
    <name>A0A8I6YPR5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123412657</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961535.1">
        <property type="nucleotide sequence ID" value="XM_045105600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPR5"/>
    <dbReference type="SMR" id="A0A8I6YPR5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717140"/>
    </dbReference>
    <dbReference type="GeneID" id="123412657"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412657"/>
    <dbReference type="OrthoDB" id="1931904at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025520">
        <property type="entry name" value="DUF4408"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098">
        <property type="entry name" value="COTTON FIBER (DUF761)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098:SF103">
        <property type="entry name" value="DUF4408 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14364">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4408" evidence="3">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="244" mass="27199" checksum="44B5C1BF4C813774" modified="2022-05-25" version="1">MQAAPMYALWSTVHGWFTPWVLFLVLNLVIATIFVTSMATSSAGSGEGVPAGPVGERRRLSRVDSVAIDRPHFSAPAPEAPMTGVLDLGHPNEQPPPLEMEAEDEGEHDAFTNEDEHEEHVHMERGMSETAVEAELPRLPTWLPKSASDKSAFAHFVDGVDTEVVEAHRPATTRDAERRRPQVAEPEEPASEVEIKEAGGEVDARADEFINKFHHQLKMQRMDSFMRSRNTLHRRRATVVPEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YR81</accession>
    <name>A0A8I6YR81_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YR81"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0566850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0566850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0566850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0566850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0566850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28990" checksum="A7F7CDBCD025EAAE" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYRGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRMKLYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YX05</accession>
    <name>A0A8I6YX05_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">NAD(P)H dehydrogenase subunit 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">NADH-plastoquinone oxidoreductase subunit 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I subunit 5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YX05"/>
    <dbReference type="SMR" id="A0A8I6YX05"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0813870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0813870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0813870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0813870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0813870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0813870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002128">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chlpt_su5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01010">
        <property type="entry name" value="Proton_antipo_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01435">
        <property type="entry name" value="NPOXDRDTASE5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="9" id="KW-0874">Quinone</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="22">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone/plastoquinone oxidoreductase chloroplast chain 5 C-terminal" evidence="23">
        <location>
            <begin position="195"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031649"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0813870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="486" mass="54180" checksum="8478BF2D63D11F55" modified="2022-05-25" version="1">MEGPTPISALIHAATMVAAGIFLLARLLPLFISLPLIMSFISLVGTITLFLGATLALAQRDIKRSLAYSTMSQLGYMMLALGIGSYQAALFHLITHAYSKALLFLGSGSVIHSMEPLVGYSPDKSQNMVLMGGLRKYIPITRTTFLWGTLSLCGIPPLACFWSKDEILSNSWLYSPFFGIIASFTAGLTAFYMFRIYLLTFGGYLRVHFQNYSSTKESSLYSISLWGKRIPKGVNRDFVLSTTKSGVSFFSQNIPKIQGNTRNRIGSFTTSFGAKNTFAYPHETGNTMLFPLLILLLFTLFIGFIGISFDNGGMDNGIAELTILSKWLTPSKNFTQESSNSFVNSYEFITNAISSVTLAIFGLFIAYIFYGSAYSFFQNLDLINSFVKRNPKKEFLDQVKKNIYSWSYNRGYIDIFYTRVFTLGIRGLTELTEFFDKGVIDGITNGVGLASFCIGEEIKYVGGGRISSYLFFFLCYVSVFLFFFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z333</accession>
    <name>A0A8I6Z333_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z333"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0668970.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0668970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0668970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0668970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0668970"/>
    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0668970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="20832" checksum="AD62C255B3DB3587" modified="2022-05-25" version="1">MWHYTGPEDSTRTNVVGVPEERVTSWVLQIKGPCENPKGARRVKPYSADHPPPNQAWTNWFSPFSNGNPEEEEEEGSQEGSVESAEYVSDSGESEEEDSEEEEEDEEQDSPPPRPEHRTKRRHEPAVPSAPPALSSAPPAAPVVPGVRGTKRARDAAAEPAGQSSRAPKPSGPKPRKALPRMRVAIPVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z883</accession>
    <name>A0A8I6Z883_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123407674</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with ARAC5 and ARAC10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_045100860.1"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:kinase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="entry name" value="Receptor-like cytosolic serine/threonine-protein kinase RBK1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF7">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
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        </location>
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        </location>
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        <location>
            <position position="250"/>
        </location>
        <ligand>
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        </ligand>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="536" mass="58404" checksum="3DE4B83DCC546309" modified="2022-05-25" version="1">MATTEGGCGGGGAERAEDSLPASGEEVQGVNQEEGRRSYGEPADEDCGSIGSVTDAAVPSEDPSEGQSNDSSDSQCAGSDGGCKEVPEVYSKSGGNDENSECTDQSSPRAVLDISVSGSVDSDESSSVEQPAVPSRSVQPLQWRNLISGLILSRKKLMARAVTFPQRSKNTGLKRYLGRMRSGKNQMDCSAIAPEIFPEIEKWRPSWRSFDYDELCAATDRFSSDNLIGKGGHAEVYKGQLADGQFVAVKRLTKGGNKEDRISDFLSELGIIAHVNHPNAAQLLGFSVEGGLHLVLQFSPHGSLASLLHGAKGALRWKARFNIALGVAEGLFYLHEGCHRHIIHRDIKASNILLTEDYQPQISDFGLAKWLPDKCTHQVVFPIEGTFGYMAPEYFMHGIINEKTDVFAYGVLLLELVTGRKAVDSSRQSLVIWAKPLLESNNMKGLVDPSLDAGYDLEEMALTLAVASMCIHHSANLRPSMKSVVRFLKGDRESLELMGKPKPTKPPMFDSCDSEDYTRTSYLNDLDKHKQLALEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z8V3</accession>
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        <recommendedName>
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        </recommendedName>
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        <lineage>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="5" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the PPC synthetase family.</text>
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            <end position="85"/>
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            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0745690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="403" mass="44174" checksum="9C8FA4D2E8CCF521" modified="2022-05-25" version="1">MVARLTPDQKVACSIHVGFNSPNKKSGSFFFPSPSFFLFLFPFLFHPSSLRFAPRHWCHSPPSPPARQPPAMDPPTSEAHRSSDDEAAGFFRAAPPLRGRDAVAASLAAFVARHHAGTGGGGGGGSPPAVAGVVCVTSGGTTVPLEQRCVRYIDNFSSGQRGAASTEYFLKAGYAVVFVHRRGSKQPYCRFLPEDSFLDLFELGEGSEIQVPESHTAVVKTAISSYRKAIDEGLLLKLPFTTIFEYLQLLQLVSTAMNCLERHGMFYFAAAVSDFYVPWESMAKHKIQSAGGPLNMQLNQVPKMLFILRNHWAPSAFCVSFKLETDPDILIQKAEAALRKYGMNVVVANELANYKDVVVMVTSSGKTTVSRRSKEDDLEEQLIGLLVKMHSDHAKQSNPDQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B136</accession>
    <name>A0A8I7B136_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123438783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044971738.1">
        <property type="nucleotide sequence ID" value="XM_045115803.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0056800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0056800.1"/>
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    <dbReference type="GeneID" id="123438783"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2018506at2759"/>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36356">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36356:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0056800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="249" mass="27649" checksum="4A739B77707D1E95" modified="2022-05-25" version="1">MASTLAPPRWHHPPPPSTGTGPGRLLHLSRSAVPARRGQLRRASTVSPRAFFGRADLDGLLQRAWRGANAGAERLSFEARQAAQRLDGRYSISRRVAEAARAARERAAEIDAELGVGRRWRSFSVDFSRSWPRYRRELSDFLATPIGRALSTLTFVWLALSGWLFRIFIFSTFVLPFAAPLLLGTFANRVAIEGSCPACKRRFVGYRNQVIRCMNCQNIVWQPNSRSSGGGGGSRSSASDVIDVEFEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3J8</accession>
    <name>A0A8I7B3J8_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123413004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961893.1">
        <property type="nucleotide sequence ID" value="XM_045105958.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I7B3J8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066720"/>
    </dbReference>
    <dbReference type="GeneID" id="123413004"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0053290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0053290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0053290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0066720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066720"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
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        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="13542" checksum="6BEE87B35C83FE35" modified="2022-05-25" version="1">MDRRKIRVLLVEDQEIHRVVARAVLRAAGVEADEAKNGAEAVRRVRDGGAGAYDLILTDKQMPVMDGHEATRQIRAMGVTTTIVAVSSDSLPSDVQAFIAAGADDFTPKPLTKEKLGNILSKFGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3L4</accession>
    <name>A0A8I7B3L4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123427441</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967422.1">
        <property type="nucleotide sequence ID" value="XM_045111487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3L4"/>
    <dbReference type="SMR" id="A0A8I7B3L4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186650"/>
    </dbReference>
    <dbReference type="GeneID" id="123427441"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0154550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0154550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0154550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0186650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427441"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000192">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000738">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000404">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF642">
        <property type="entry name" value="INACTIVE LEUCINE-RICH REPEAT RECEPTOR KINASE XIAO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035154703" description="Protein kinase domain-containing protein" evidence="18">
        <location>
            <begin position="37"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="781"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="863"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="820"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1171" mass="123354" checksum="6977275CC74B92FE" modified="2022-05-25" version="1">MTKPRDKAARPSSTLARAMPPLFLLLMVAAAAGAGAATPPPPVRRAAEVQAEIDALLAFRAALRDPYAAMAGWDASSPSAPCSWRGVACNAASGRVVELQLPRLRLAGPVSPALASLRHLQKLSLRSNALTGAIPPALARLASLRAVFLQDNALSGPIPPSFLANLTGLETFDVSANLLSGPVPPALPPGLKYLDLSSNAFSGTIPAGAGASAAKLQHFNLSFNRLRGTVPASLGALQDLHYLWLDGNLLEGTIPSALANCSALLHLSLRGNALRGILPAAVASIPSLQILSVSRNLLSGAIPAAAFGGERNSSLRILQLGDNQFSMVDVPGGLGKGLQVVDLGGNKLGGPFPTWLVEAQGLTVLNLSGNAFTGDVPAAVGQLTALQELRLGGNALTGTVPPEIGRCGALQVLALEDNLFSGEVPAALGGLRRLREVYLGGNSFEGQIPADLGNLSWLETLSIPNNRLTGGLPNELFLLGNLTVLDLSDNKLAGEIPPAVGSLPALQSLNLSGNAFSGRIPSTIGNLLNLRALDLSGQKNLSGNLPTELFGLPQLQHVSLADNSFSGDVPEGFSSLWSLRHLNISVNSFAGSIPATYGYMASLQVLSASHNRISGEVPAELANCSNLTVLDLSGNHLTGPIPSDLSRLDELEELDLSHNQLSSKIPPEISNISSLATLKLDDNHLVGEIPASLANLSKLQALDLSSNSITGSIPVSLAQIPSLVSFNVSHNDLAGEIPPVLGSRFGTPSAFASNRDLCGPPLESECGEYRRRRKRQRLQRLALLIGALAAASLLLLLLCCCCVFSLLRWRRRFVERRDGVKKRRRSPGRGSGSSGTSTESQTKLVMFNSRITYADTVEATRQFDEENVLSRGRHGLMFKACYSEGTVLAILRLPSTSADGAVVVEEGSFRKEAESLGRVKHRNLTVLRGYYAGPPPDVRLLVYDYMPNGNLATLLQEASHQDGHILNWPMRHLIALGVSRGLAFLHQSGVIHGDVKPQNILFDADFEPHLSDFGLEPMVVTAGAAAAAAAASTSATTPVGSLGYVAPDAATAGQATREGDVYSFGIVLLELLTGRRPGMFAGEDEDIVKWVKRQLQCGAVAELLEPGLLELDPESSEWEEFLLGIKVGLLCTASDPLDRPAMGDVVFMLEGCRVGPDIPSSADPTTQPSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B440</accession>
    <name>A0A8I7B440_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123449864</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983155.1">
        <property type="nucleotide sequence ID" value="XM_045127220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B440"/>
    <dbReference type="SMR" id="A0A8I7B440"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0086140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0086140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0086140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449864"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0070850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0070850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0070850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0086140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0086140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0086140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449864"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368:SF4">
        <property type="entry name" value="OS10G0403700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="159"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0086140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="39832" checksum="E1B9D7D9BA629364" modified="2022-05-25" version="1">MSSAAPAPAPEEQPPRPVRFGIMGCASIARKLARAMLLAAPAAAVAAVGSRSEAKARLFAADNGLPADTRLHGSYEALLDDPAVDAVYLPLPTSLHVQWATAAAARGKHVLLEKPTALCAADLDAILAACDASGVQFMDSTMWMHHPRTAKMRDLLLADVGDVRLINSLFSFRANEDFLQNDIRVKPDLDALGALGDAGWYCIRAILWAVDYELPKNVIALPDPVKNQAGVLIACGATLYWADGKMATFHCSFLTNLTMDLTVVGTDGTLHVTDFVIPYEEKSGPFSVASKSNFAELHTGWAPQPSKHVVMTDLPQEALMVKEFCRLVQGIRDAGAKPENKWPAITRKTQVVMDAVKASIDNGFKSVDVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7T5</accession>
    <name>A0A8I7B7T5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7T5"/>
    <dbReference type="SMR" id="A0A8I7B7T5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0155760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0155760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0155760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0188080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF57">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035314439" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="26"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="40138" checksum="0B3B6B4E2EF16A7F" modified="2022-05-25" version="1">MAGHGGPLALVALCVLELALRGAGAGEAPAPGPALGLAPGPAPERLVPAMFVFGDSTVDVGNNNLLPDCTPACRANYPQYGVDYPSQEPTGRFSNGFNLADQLAQFLGFDESPPAFQSLPAKGIAAQMKNGVNFASGGSGLQNKTGQKLCGQLFSMADQVEKFTSAVKTMGQDSGDLISRSLFFISVGSNDLFEYADPDSPPADRNDTAFLESLVAYYRGYLQELHAAGARKFSIISPALVGCCPSQRAIAKKHDDTDEFGCFGAANNLSRQLYPMLASMLKDLSRDRAGMNYSLCDSATMAETIFKPGGGAGFDLTVLDTACCGGAGKCNSSAKLCPNHDNYMFWDSYHPTKAASALAAMALFSDPGMYVHPMNVGSLAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9I3</accession>
    <name>A0A8I7B9I3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123399945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Zeta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044950271.1">
        <property type="nucleotide sequence ID" value="XM_045094336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9I3"/>
    <dbReference type="SMR" id="A0A8I7B9I3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496630"/>
    </dbReference>
    <dbReference type="GeneID" id="123399945"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0411930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0411930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0411930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0496630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399945"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03191">
        <property type="entry name" value="GST_C_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000100">
        <property type="entry name" value="Glutathione S-transferase Z1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000017">
        <property type="entry name" value="Maleylacetoacetate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005955">
        <property type="entry name" value="GST_Zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034330">
        <property type="entry name" value="GST_Zeta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01262">
        <property type="entry name" value="maiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF19">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="88"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="214" mass="23908" checksum="7BCC6A185C348CE7" modified="2022-05-25" version="1">MSAAAGKPILYSKWFSSCSHRVRIALNLKGVDFEYRATNPMTDPDYEKINPVKYVPALVDGDFVVSDSFAIILYMEDKYPQCPLLPRDLKKKALNLQIASIVCSSIQPLQSYAVIRSFHGTMDANESLEMVEHYIDKGFRAIEKLLEGCDSKYATGDEVQMGDVFLAPQIHAGVTRFQIDMSKYPILARLQDAYNEHPAFQAALPANQPDAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9R1</accession>
    <name>A0A8I7B9R1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123443470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044975777.1">
        <property type="nucleotide sequence ID" value="XM_045119842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9R1"/>
    <dbReference type="SMR" id="A0A8I7B9R1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0255500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255500"/>
    </dbReference>
    <dbReference type="GeneID" id="123443470"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0255500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0255500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0255500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443470"/>
    <dbReference type="OrthoDB" id="340227at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006607">
        <property type="entry name" value="DM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF101">
        <property type="entry name" value="LA-RELATED PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21071">
        <property type="entry name" value="LARP1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00684">
        <property type="entry name" value="DM15"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="6">
        <location>
            <begin position="324"/>
            <end position="413"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
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        <location>
            <begin position="412"/>
            <end position="460"/>
        </location>
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        </location>
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            <begin position="709"/>
            <end position="728"/>
        </location>
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        <location>
            <begin position="937"/>
            <end position="960"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
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        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
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            <begin position="412"/>
            <end position="440"/>
        </location>
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            <begin position="442"/>
            <end position="460"/>
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        <location>
            <begin position="535"/>
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        <location>
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        <location>
            <begin position="944"/>
            <end position="953"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="960" mass="106249" checksum="4C8B7D96AE8826AB" modified="2022-05-25" version="1">MEEPNAGGGAVAAPEVIADPGVGGASGGVGSPASPWRKTTPPPVAGEAAVMGAESWPALEEARQKVVVEPTGKPVPGNAAGGDLAKGPEVQQAPPSPSQVSNRMHKFDGHANPNKNHQAHYKNGPKRRPPGANDAPPYPATMPYHQHPGQPIFYPVLPNPMMLHEYPYQPFAVPVPNHDPHVGKSGFESTTPPFAPVDQVGGNEGNRPMPPQTRGDHHAWRPAVGTHGARPHSSVEGRGHFNHTWQSHQTFGTRENTSMPHGVGPRTFVRPMAHLPPTLGYINGPSYPGPMPPMYYYMPAPPMEAMRGPPRFVQNQPAPQPVLSPEATELRAKILAQVEYYFSDTNLERDGFLKSLMDEQGWVPISKVADFNRLKRITTDVHLIVDVLASSNLLEVQDDKIRRRSDWSKWVSSSGATSVQSPSSTASMDNIMGESNTGGFSNKDDAFSSDQKKHPHPHDNKCNIDGAAIEAIVPDEELPNDAHSCSLDKGLSAMTIGGKPKSISTFSGNSRTHEAAVRTGDVKVPKVNTKMKVPESQSGSGFRNLPSDSPSFSGDQSTFMLDEELELEHAEHSRDGVYSHKRVDDEEDDFFVDDQEVNRLIIVTQDTRSEKDDKSCSIVSQAFSTEEASRINDALYYYESVHDRRTNNQRSSQADTADVDSKQFVGAKGNHVSIGTNGIEEAGQPIPRRRQSKGNRKTHTSHKQRFFAGNFANSPNSRSHYGGVSESPPSNSIGYFYGSTPENHSSYKSSKLSSSPHGIPTGSSPIGSVPKSFPPFQHPSHQLLEKNKFQQQRYNKFKNKCVAERKKLGIGLSEEMNSLYRFWSYYLRDNFNEDMYKHFKNFAVDDAVANYRYGLECLFRFYSYGLEKNFQRNVYEDFEQLTLEFYHKGDLYGLEKYWAFHHFRKQDSTPISKHAELERLLREEFRTIEDFKAQRAADKGTVGSSSKSAVAASHNNAEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9X1</accession>
    <name>A0A8I7B9X1_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
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            <end position="230"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0266250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24817" checksum="82E520413DCDB995" modified="2022-05-25" version="1">MPHLYDLYGMAHTASYKKANAFSESDLDDPNNFTNISSHQKLVAYRDAGKATKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKARQSSSCPEITRRPRPVELAIEAALQKERAANQAALEKERLASQAALQAALGERDHTTARLLEEERARNEAGQRALYELFVGLCEKSGQVPPPMPVFSSIRTNNSRAASHDPSPGQPSPNEVGASNRGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I7BB36_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
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        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0550960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25154" checksum="06D1C35B9424123B" modified="2022-05-25" version="1">MPTCFEEVLAHGTTMLDVVYTNESREVPFFLEQLKEKWLDVAMDHEKFLGLDLEYTADQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSVITFASVDIRNDKLKMRHSFGIEIPTGCLIDLQTVFRLRHVRTSMAHMAVALIDEEYGDMKTSFPKSQHKHWEKGPLDHINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB70</accession>
    <name>A0A8I7BB70_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB70"/>
    <dbReference type="SMR" id="A0A8I7BB70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555190"/>
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            <end position="165"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="19258" checksum="D4B214DD0E825015" modified="2022-05-25" version="1">MKSTQLSESLNSDLKDYLKSNLDILRFFKHVERTVQGKRDKERWTREARSGTIQNSHGNIVLEDPRSEDRQQLKFFMHDFLDIASQAVASEECIKLVDDTLKILRKQVEDKAPTTVCRTEAISQEPNICLQTACLKKKEIQKKTSKNKISWLEKQHPSKRKKRDS</sequence>
</entry>
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        <name evidence="3" type="primary">LOC123406273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Hordeum</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556640.1">
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    </dbReference>
    <dbReference type="GeneID" id="123406273"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0462350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0462350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0556640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556640.1.CDS1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
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            <begin position="1"/>
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        </location>
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        <location>
            <begin position="1"/>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="415" mass="45955" checksum="01308AE7581D5BC9" modified="2022-05-25" version="1">MQADLDRSVRRRQDSSDRQIMSPPAQLPDDLIHYSILSRLPFRLLLRLAPVCKAWRHLILHDPVFARVQTQCPSPASGVLARFHKGRIEVLTPGSTSAAVAPPDASLSFLPVVDAHRQYLRLCSTTSGLILITTGSTFWVVNPATRAFRDVPYAGEGAFRASLAYDPATAHQESYHLVVPARQNLELWRFWIFSFSSLSRGWRASWATVRMPPYSYVHPKALYLGGLSYWLCDRDDVLWYNYGADAAGKLPPPPHGKKRPSSLLDGGGYMEGNGELVAWHGRIGMVSASAAMLAVWALSSPPSGSASPPARWEMVHQRSWDEIPGMAPPVSRFLWSVVPAGVDVGAEGVLGPAVRIGYRQRGCVGGGDNENAWRREVLRYDMRTDATTTVAELFGREKHDDFVVFGYHSSMAPLY</sequence>
</entry>
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    <accession>A0A8I7BBA8</accession>
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        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A8I7BBA8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0560240.1">
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="OS04G0422900 PROTEIN"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00006958"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0560240.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="18117" checksum="1674BAB8BF4215C0" modified="2022-05-25" version="1">MVVCDFDLNFTFISCAWEGSATDARVLRSALNNGVQVPPGKFYLVDGGYANTPYFLAPYSGVRYHLKEFARGRRRPQNHRELFDHRHAVLRNHIERALGVLKKRFPILKVGTHHRIENQVKLPAAAAILHNIFRSHQGDERWCNDQDAVLSDLGVEAPE</sequence>
</entry>
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            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Platzer M."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
        </source>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979642.1">
        <property type="nucleotide sequence ID" value="XM_045123707.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045123708.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045123709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BBH5"/>
    <dbReference type="SMR" id="A0A8I7BBH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0334450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0334450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0334450"/>
    </dbReference>
    <dbReference type="GeneID" id="123447132"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278900.1"/>
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    <dbReference type="KEGG" id="hvg:123447132"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
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    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="123395550"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0371470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0371470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0371470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0448500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0448500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0448500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395550"/>
    <dbReference type="HOGENOM" id="CLU_095133_1_0_1"/>
    <dbReference type="OrthoDB" id="691601at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990052">
        <property type="term" value="P:ER to chloroplast lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010441">
        <property type="term" value="P:guard cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009667">
        <property type="term" value="P:plastid inner membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009666">
        <property type="term" value="P:plastid outer membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053288">
        <property type="entry name" value="TGD_Bridge_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34201">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34201:SF12">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ87065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0448500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="98" mass="9932" checksum="71BF0110FB9FDF12" modified="2011-05-31" version="1">MVVSTFSGPGIGLGFGVGCGFGVGWGFGGMPLNVFGLGIGGGCGVGLGLGWGFGNAYGCQYRSSRVQFQGIEFQKKSEGDEAPKLVSSGLVEKSRPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0P3</accession>
    <name>F2D0P3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123452737</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357450">
        <property type="protein sequence ID" value="BAJ88664.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985382.1">
        <property type="nucleotide sequence ID" value="XM_045129447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0P3"/>
    <dbReference type="SMR" id="F2D0P3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519820"/>
    </dbReference>
    <dbReference type="GeneID" id="123452737"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0432120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0432120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0432120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519820"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039235_1_0_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16525">
        <property type="entry name" value="RING-HC_PCGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044807">
        <property type="entry name" value="DRIP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46293">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE DRIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46293:SF4">
        <property type="entry name" value="OS03G0798200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ88664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="396" mass="43654" checksum="E6D43E2BAD9C37D7" modified="2011-05-31" version="1">MQDAVLACPAPSDGGAGGGDGEVGSAGPGVVMVKRAALVERLTCPLCRRLLRDAATITECLHTFCRKCISKEFIDKEICYCPTCNIDLGCAPEEKLRVDHSLQYVRSKIFPSKRRKVVDQEAISPFASPAKRKERSLSSLTVHGPRVSIQKCLTKRRTKASCLRSLSLGSKDATKKVGGWKPLASHIRVGKNKKSLKSGSEEDNRTGIKSGDPEHGAPSNEAKASLLNRFLESKEYLTRKENLVKKTGSKKVFTLKGKKKIFKAKQPRKKRRLQALWFYLVAAFDQKGQPPLPQLPTKFLRIKDVELPASFIQKYLVQKLNLSSEAEVELMCVGKKVDPAITLHDIMECYLDKGPNGWVRSSVGSPATGFITTVFYSRPELPTPTTTTTTTTTTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2D275</accession>
    <name>F2D275_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123445230</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357982">
        <property type="protein sequence ID" value="BAJ89196.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK367478">
        <property type="protein sequence ID" value="BAJ98681.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK370727">
        <property type="protein sequence ID" value="BAK01926.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978130.1">
        <property type="nucleotide sequence ID" value="XM_045122195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D275"/>
    <dbReference type="SMR" id="F2D275"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0072890.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0072890.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0072890"/>
    </dbReference>
    <dbReference type="GeneID" id="123445230"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0058890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0058890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0058890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0072890.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0072890.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0072890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445230"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
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                <person name="Hori K."/>
                <person name="Itoh T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        </source>
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    </evidence>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    <organism evidence="6">
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="3">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="molecule type" value="mRNA"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
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            <end position="64"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ92410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089710.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="87" mass="9781" checksum="0140596BFD583B46" modified="2011-05-31" version="1">MSSGSSRSTSPSSDSEWSKKENKMFEEALAYYGEGAPNLWEKVACAMGGTKSPDDVRRHFQILVDDVKNIQSGRIPFPKYKTQGFWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F2DIX8</accession>
    <name>F2DIX8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123448527</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADAGIO family.</text>
    </comment>
    <dbReference type="EMBL" id="AK363846">
        <property type="protein sequence ID" value="BAJ95049.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981385.1">
        <property type="nucleotide sequence ID" value="XM_045125450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DIX8"/>
    <dbReference type="SMR" id="F2DIX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369880"/>
    </dbReference>
    <dbReference type="GeneID" id="123448527"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0308090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0308090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0308090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0369880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0369880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0369880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448527"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009902">
        <property type="term" value="P:chloroplast relocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001007">
        <property type="term" value="P:negative regulation of cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009911">
        <property type="term" value="P:positive regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000005">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000026">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175:SF2">
        <property type="entry name" value="ADAGIO PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175">
        <property type="entry name" value="BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="11" id="KW-0157">Chromophore</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="59"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ95049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0369880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="626" mass="68728" checksum="6572165032E53691" modified="2011-05-31" version="1">MFDGVDRGALVTVKRMRLCAMEEDEEEVGMEVDGEEEEEGTGGWAWGAPGLGMAGQPGEQRAAAIVVADAVEPDFPVIYVNAAFEAATGYRAHEVLGRNCRFLQFREPRAQRRHPLVDPMVVSEMRRCLSDGIEFQGELLNFRKNGAPVNNRLRLIPMHGDDGAFTHIIAIQLFSDANIDPSNISYPIYKPQSSHRLSIQDMNRASHETSPKVQCSEYCAIFQLSDEVLAHNILSRLSPRDVASVGSVCTRMHLLTKNDLLRKMVCQNAWGRDVTGRLEMSTKMLGWGRLARELTTLEAASWRKFTVGGRVEPSRCNFSACAVGNRLVLFGGEGVNMQPMSDTFVLNLDAPKPEWCRVKVSASPPGRWGHTLSWLNGSWLVVFGGCGQQGLLNDVFVLDLDAQTPAWREIASDGPPLPRSWHSSCTLDGSKLVVSGGCAESGVLLSDTFLLDLTKEKPAWKEIPTSWSSRLGHTLSVYGKTKIFMFGGLAKSGSLRLRSSDAYIMDVGEENPQWRQLATTVFPSGCPPPRLDHVTVSLPCGRIIIFGGSIAGLHSPAELFLLDPAEEKPTWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGGHSGEEWILNELHELCLASRPDEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2DNJ3</accession>
    <name>F2DNJ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123447741</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="6"/>
    <dbReference type="EMBL" id="AK365461">
        <property type="protein sequence ID" value="BAJ96664.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK375703">
        <property type="protein sequence ID" value="BAK06898.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980324.1">
        <property type="nucleotide sequence ID" value="XM_045124389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DNJ3"/>
    <dbReference type="SMR" id="F2DNJ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341330"/>
    </dbReference>
    <dbReference type="GeneID" id="123447741"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0284840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0284840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0284840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0341330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447741"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22586">
        <property type="entry name" value="Rcat_RBR_ARI1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16773">
        <property type="entry name" value="RING-HC_RBR_TRIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="137"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="141"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ96664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="547" mass="62542" checksum="97DF8E332DF1F5EF" modified="2011-05-31" version="1">MASDDDDCYYYCDDDGDGDGEADEEAADWNGLAVGADEDDLGLLEEEPPPPERRVDCWAITGDTLSAAQQEDLSIVMNLLNIKQHQARSLFIFHRWKIDCIYDCLDRKGRDRMLREAGIVLQEKNSMLIGASRTPSTSVQCNVCFDDDLSPAAVSTMDCGHCFCNDCWTEHFNAAIDGGKKQIRCMEVKCLAICDEGIVQRLLGQKYPDAAKRFNRFLLESYLEDNDFVKWCPSIPHCGRAIRVGTGDRYCEVKCLCGVSFCFNCTEQTHSPCPCTIWKKWNTRIHGESENIKWIVKNTKSCPKCFKPIEKRDGCNLVKCKCGQYLCWLCGGPTGRAHTWTNIEGHSCNRYKESKDKVDTGRWQLERYAHYCNRFKIHEDSYKEQHEKLGPAIKEKVKQLESNHLRPRLIRDGDWLTDAHQRLLWSRQVVSRSYAFAYHMFGGELRAHRSERGNLVPAQNLFENQQEQLEQHVEQLSKVLVTDIPALPDQEIVQVKQDVVNLAKILERLCGEMYTCIQDELLPLLTVPMDIAAYTPDGPVRAKVFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2DPI4</accession>
    <name>F2DPI4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123452282</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365802">
        <property type="protein sequence ID" value="BAJ97005.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984851.1">
        <property type="nucleotide sequence ID" value="XM_045128916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DPI4"/>
    <dbReference type="SMR" id="F2DPI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0470980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0470980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0470980"/>
    </dbReference>
    <dbReference type="GeneID" id="123452282"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0390000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0390000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0390000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0470980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0470980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0470980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452282"/>
    <dbReference type="eggNOG" id="KOG1989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DPI4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000349">
        <property type="entry name" value="probable serine/threonine-protein kinase DDB_G0280111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF103">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="32"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="395"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="486"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ97005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0470980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="693" mass="76216" checksum="B53067E038C06A3B" modified="2011-05-31" version="1">MWRPFRQLMPKEHSGPSGGLEGRTIDVGNVKVHVREAIAEGGFSCVYLARDLTNPAKHYALKHVIVQDKESLDLVHKEITVMRALKGHPNVVTLVAHTILDMGRGREALLLMEFCERSLVSALDARGTAGFYDDEQVALIFRDICNAVFAMHCQTPPVAHRDLKAENVLLGADGAWKLCDFGSVSTNHKCFDKPEERGIEEDIIRKHTTPAYRAPEMWDLYRREVISEKVDIWALGCLMYRICYLKSAFDGESKLQILNGNYRIPELPKYCSSITSLIEEMLQSSPDARPDITQVWFRVNELLPLELQKDLPDGSPSGSAFELQITEHEGPSASPSRDNVRNTPPKGPSSLRLQAPTESKGSVGAFWSTQHAQELAFADNKGSALDQEPIYDGTAKQPQAKNQNTAQNTYRQSLSASVDSSPGDFEIRFSPNGSEYGLEKTQNSKTDNKTSTQTAIFSSFVADFDNVKVISEDNASSLNTTSKLKEQQLEAEVTLLKEQLKIANLEKEEISLKFDRLSAICSSQRREIQELKQALAIASAAAPVKELKENLKAEFSPPSTSLDTPPREKIAGTPPELRQGLFTSSPGTPSPDLKPWSAFPEEPKAQAAVKSAHPRSVRTLRASNSNKAGSLSQSKTNSGADPFAFGQDSFKAVPSGTVPSEILNLGNASQSLNTLKADVKKDEPYQPAGWTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F2DSS3</accession>
    <name>F2DSS3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Laccase</fullName>
            <ecNumber evidence="5 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123448225</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Secreted</location>
            <location evidence="3 13">Extracellular space</location>
            <location evidence="3 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5 13"/>
    <dbReference type="EMBL" id="AK366941">
        <property type="protein sequence ID" value="BAJ98144.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980989.1">
        <property type="nucleotide sequence ID" value="XM_045125054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DSS3"/>
    <dbReference type="SMR" id="F2DSS3"/>
    <dbReference type="PaxDb" id="4513-MLOC_50936.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0357240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0357240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0357240"/>
    </dbReference>
    <dbReference type="GeneID" id="123448225"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0298030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0298030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0298030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0357240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0357240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0357240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448225"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_6_3_1"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DSS3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF370">
        <property type="entry name" value="LACCASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11 13" id="KW-0186">Copper</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019619" description="Laccase" evidence="13">
        <location>
            <begin position="27"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="38"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="164"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="415"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ98144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0357240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="564" mass="62084" checksum="740D56334993A2E9" modified="2011-05-31" version="1">MAMPRRLDQLLLPLMVSCFLLHQALSVHAIARHYKFNVVMRNMTLLCSTKSILTVNGKFPGPTLYAREGDNVLVKVVNHAPHNVTIHWHGVRQIRTGWYDGPAYITQCPIQPGSSFLYNFTVTGQRGTLLWHAHINWLRATVHGAIVILPKLGVPYPFPAPHKEAVVVLGEWWKADTETVINQAMQLGVGPNISDSHTINGHPGPMSACASSQDGFKLDVENSKTYMLRIINAGLNDDLFFKIAGHGLTVVEVDAVYTKPYKTDILLITPGQTTNVLVTADQSAGRYLLSVSPFMDAPLQVDNKTGTATLHYANTVSTTARLTLVNPPPQNATPIASTFSESLRSLNSKEYPANVPQTVDHSLFFTIGVGVNPCANCINGTRVVGTINNSTFVMPSTPILQARYYSIPGVFTEDFPATPPHKFNYTGSGPNNLQTMNGTRVYRLPYNASVQVLLQDTGIISTESHPIHLHGFNFFVVGRGIGNYNPKSSPFTFNLIDPVERNTIGVPTGGWTAIRFRADNPGVWFMHCHFEVHTSWGLKMVFVVDNGKRPSETLIPPPKDLPQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTC3</accession>
    <name>F2DTC3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123439418</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367141">
        <property type="protein sequence ID" value="BAJ98344.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972070.1">
        <property type="nucleotide sequence ID" value="XM_045116135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTC3"/>
    <dbReference type="SMR" id="F2DTC3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233580"/>
    </dbReference>
    <dbReference type="GeneID" id="123439418"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439418"/>
    <dbReference type="HOGENOM" id="CLU_000134_44_6_1"/>
    <dbReference type="OrthoDB" id="412869at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051616">
        <property type="entry name" value="Cul2-RING_E3_ligase_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224:SF34">
        <property type="entry name" value="OS01G0171100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="143"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="241"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="405"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ98344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="466" mass="50165" checksum="9FD79EE1F201CF4E" modified="2011-05-31" version="1">MDMPTAACSPSSSAQPPPLPSAVDVAVDARNDEVRRRQLLDAAIDGNLDLLARMALELLPPAPSTTGADATGVWSTCGRALHLAAANGRTDVCRYLVQDLGIPVDSLSDERETPLLLAATFGHTATAAWLLERGASPRAPDVDGETPLHWAAYNGDRDLAMLLLYKGADVSAANPRGTALHVAAMRGCPEVVRVLLRHGADPNKVASRVFTPLVSSLLGGSVECMKLLIEAGANVHAGGFTGATPLLLACSRRGNVGFVKCLLKAGADPNLPDELGRLPIEIAAVQAEKKLVRLLFPVTRCPPNMLDWSVAGIMSYVKSAAYKERVRKDSCKRKAELKLEGNKAYTVKDYDTAILMYNLALKFDDANDIDASIYSNRSLCWLRLGVGDEALSDAQACIRFWPDWGKGYYREGEAFRFLEDYTSAFASFMKASELDPQNPKIAGALRDLWERAKDVSGSRVSGPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0H1</accession>
    <name>F2E0H1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123452520</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369642">
        <property type="protein sequence ID" value="BAK00843.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372214">
        <property type="protein sequence ID" value="BAK03412.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985113.1">
        <property type="nucleotide sequence ID" value="XM_045129178.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985114.1">
        <property type="nucleotide sequence ID" value="XM_045129179.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985115.1">
        <property type="nucleotide sequence ID" value="XM_045129180.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985116.1">
        <property type="nucleotide sequence ID" value="XM_045129181.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985117.1">
        <property type="nucleotide sequence ID" value="XM_045129182.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985118.1">
        <property type="nucleotide sequence ID" value="XM_045129183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0H1"/>
    <dbReference type="SMR" id="F2E0H1"/>
    <dbReference type="ESTHER" id="horvv-f2e0h1">
        <property type="family name" value="TGL-GrainShapeFactor"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515230"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515230.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515230"/>
    </dbReference>
    <dbReference type="GeneID" id="123452520"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0428110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0428110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0428110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0515230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0515230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515230.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452520"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF13">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="64"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK00843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="348" mass="38447" checksum="F7550994309C06D7" modified="2011-05-31" version="1">MPAPPQPAGGRRRWKHRLSPTLARDRCYARAFRSAGLRQAAVPLPDGAVVHFWLPRPDPALHPVLLLHGFGANATWQWAPFLRPLIAAGLAPFVPDLVFFGDSASPAADRSPAYQAASVAAAMAALPGAPQRYSVVGVSYGGFVAYHLAHAFPAVVERLVLVAAGVCLEEADLASGLFAVDDISEAASLLLPQRPEDLRRLVDLTFCKPPKFMPSCFIRDYIRVMCTDNVKEKTELLYALISGRKLSDLPKINQQTLIIWGEQDRVFPLELGLRLKRHLGDTSELTIVKDAGHAINREKPAELCRLIKNYIIDPSVKYRDDRKGCWKFALRRFAGSSLRKVDSSRPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2E6H5</accession>
    <name>F2E6H5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123452508</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371749">
        <property type="protein sequence ID" value="BAK02947.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985099.1">
        <property type="nucleotide sequence ID" value="XM_045129164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E6H5"/>
    <dbReference type="SMR" id="F2E6H5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0514950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0514950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0514950"/>
    </dbReference>
    <dbReference type="GeneID" id="123452508"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0427860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0427860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0427860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0514950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0514950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0514950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452508"/>
    <dbReference type="HOGENOM" id="CLU_094004_2_2_1"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK02947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0514950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="145" mass="16802" checksum="53E935F0AFE479B3" modified="2011-05-31" version="1">MAFMRTSSNASSGMGVAPDIRETFLELQMKKAFRYVIFKIEEKQKQVVVEKTGATTESYDDFLACLPENDCRYALYDFDFVTGENVQKSKIFFIAWSPDTSRIRAKMLYSTSKDRIKQELDGFHYEIQATDPTEVELDVLRDRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2E750</accession>
    <name>F2E750_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123439129</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK371974">
        <property type="protein sequence ID" value="BAK03172.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971813.1">
        <property type="nucleotide sequence ID" value="XM_045115878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E750"/>
    <dbReference type="SMR" id="F2E750"/>
    <dbReference type="STRING" id="112509.F2E750"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219640"/>
    </dbReference>
    <dbReference type="GeneID" id="123439129"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0219640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0219640.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0219640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439129"/>
    <dbReference type="OMA" id="STIAMAP"/>
    <dbReference type="OrthoDB" id="673365at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21037">
        <property type="entry name" value="MLKL_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059179">
        <property type="entry name" value="MLKL-like_MCAfunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146:SF17">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE-LIKE PROTEIN CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="192"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK03172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0219640.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="504" mass="54851" checksum="3C69AFC64C753DCF" modified="2011-05-31" version="1">MALAVIGDAAAVTQLIGQATGLIAKIIRAVETARQNRVECDHLVCRVSSVHGVVYNLPPYQEMAPPLKVVNHTLEEAHRMVIACRNRGAASQFFGSRRIADCFSQINLRIAADVVILNLSLCSCMARRHSNQVLHPVPNDIGVPTSTIAMAPGFSSGSLQTQMVVSQSPSQVSSVSFDNPRALTGSEIAAATSFAVELGRGSSGRVYMGRLQFQHHGTVTVAEVAVKVLEKHGRHGVEDAFVAESNILSGLRHDHIVRLVGWCAEQEYRMLVYEHMGKGTLRDQLQQLGGCSSASPVTRSWDWETRVEMLLAVARAIHYMHRGADPVVIHRNVSSSNILLDANWTLRLSGFGSAVFQAAGEDEEHGAGGQLVEEVVGTRGYIDPEYNLTNRVNPASDVYSFGVVMLEALTGRPPDVYGVTLGLLVDSALPIIRMGNLREVLDGRPSPQPTRRQLDALDLVAHTASHCLWPGRQNRLPISHVVANLQTALQMIRSNEPVSWARID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2E9D4</accession>
    <name>F2E9D4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123406029</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AK372759">
        <property type="protein sequence ID" value="BAK03956.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955467.1">
        <property type="nucleotide sequence ID" value="XM_045099532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E9D4"/>
    <dbReference type="SMR" id="F2E9D4"/>
    <dbReference type="PaxDb" id="4513-MLOC_9974.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0633000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0633000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0633000"/>
    </dbReference>
    <dbReference type="GeneID" id="123406029"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0525710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0525710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0525710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0633000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0633000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0633000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406029"/>
    <dbReference type="eggNOG" id="ENOG502QPKZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013238_2_1_1"/>
    <dbReference type="OrthoDB" id="645614at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E9D4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="6">
        <location>
            <begin position="76"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="7">
        <location>
            <begin position="379"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK03956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0633000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="484" mass="53116" checksum="32B6F75AD502441A" modified="2011-05-31" version="1">MVSSPLHPRKHVLKPLYQLLVNNFLAVVVVPVTVAGVLGKAAQLGPDELLTRLHGLRQVHIFLAVFLPLALVALSVMRRPRSVYLVDYACCRPNPNCRVSIASFTEHARHIPNLDGGGFRFLTRMLHHSGLGDQTYLHPSLHHIPPCCSLDESRDEAEQVIFAAIDDLLAKTGISPGAIDIIVTNCTAFNPTPSFTDMIINKYKLRSDIRDTHISGMGCSAGVISLEVARNLLQTVPRGGHALVVSTETTNLINYTGKNRAMLLPAVLFRMGAAAVLLSTSRSKARFRLTHVVRTLTAAQDRAYRCAYLEEDEEGQTGINLSKDLVAIAGDTLKANIVAIGSLVLPPSEKLLFALSFLARKVLSRKIKLYVPNFRTAFQHFCIHSGGRAVIDAVQTSLCLSDVDVEPSRMTLHRFGNTSSSSLWYELAYIEAKRRTRKGDRVWMVGFGSGFKCNSAVWECIIGSPGNTTTIGAPWADSIHRYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2EAG8</accession>
    <name>F2EAG8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123402158</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373143">
        <property type="protein sequence ID" value="BAK04340.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951974.1">
        <property type="nucleotide sequence ID" value="XM_045096039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EAG8"/>
    <dbReference type="SMR" id="F2EAG8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555590.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555590"/>
    </dbReference>
    <dbReference type="GeneID" id="123402158"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0461500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0461500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0461500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0555590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0555590.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0555590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402158"/>
    <dbReference type="HOGENOM" id="CLU_009301_8_2_1"/>
    <dbReference type="OrthoDB" id="1727884at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005785">
        <property type="term" value="C:signal recognition particle receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14826">
        <property type="entry name" value="SR_alpha_SRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17876">
        <property type="entry name" value="SRalpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000007">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000016">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000188">
        <property type="entry name" value="signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007222">
        <property type="entry name" value="Sig_recog_particle_rcpt_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF6">
        <property type="entry name" value="SRP54-TYPE PROTEINS GTP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04086">
        <property type="entry name" value="SRP-alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="9">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK04340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0555590.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="606" mass="65439" checksum="BE90388EA0405EAA" modified="2011-05-31" version="1">MLEELLIFTRGGLILWSLAGAAAALKGSPIDALVRSFLLEGRSASAGFTHGPYALRWAYDNALGLVLIAVYRRVLHLLYVDDLLAAISAEFARIYHPRRASYDEFGERFRQLHLEAEARASRPHSVSAKPGPADSSPGALQLNGGGGGGGGGEGEKTGDESGKDDSDGEPSRQEEHSSAADGSFNGVEENSDAGHGGAVVRTEEEGGSNNGAFNFENIKKSLFPRNDKDKRDKPPHKGKKQRLGKDQTPVSKDKKPSEKKLDYSNPADHGRRAGEQTAVNQGKSMMDKDEAVRSTTKDGWFSSMFRSIAGSNAELEESDLQPALKSLKDRLMTKNVAEEIAEKLCESVAASLLGKKLGSFTRISSSVQTAMEEALVRILTPRRSIDVLRDVHAAKDRGTPYVIVFVGVNGVGKSTNLAKVAYWLLQHDLSVTLAACDTFRSGAVEQLRTHARRLQIPIFEKGYERDPAVVAKGAIQEATRNKSDVVLVDTAGRMQDNEPLMRALSKLINLNNPDLVLFVGEALVGNDAVDQLTKFSQKLADLSTAPTARSIDGILLTKFDTIDDKVGAALSMVYVSGAPVMFVGCGQSYTDLKKLNVKSIVNTLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2EDY7</accession>
    <name>F2EDY7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123442145</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Flower</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374362">
        <property type="protein sequence ID" value="BAK05559.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974164.1">
        <property type="nucleotide sequence ID" value="XM_045118229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EDY7"/>
    <dbReference type="SMR" id="F2EDY7"/>
    <dbReference type="FunCoup" id="F2EDY7">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2EDY7"/>
    <dbReference type="PaxDb" id="4513-MLOC_15149.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0064180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0064180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0064180"/>
    </dbReference>
    <dbReference type="GeneID" id="123442145"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0051150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0051150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0051150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0064180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0064180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0064180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442145"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_7_1_1"/>
    <dbReference type="OMA" id="VVCAWAF"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF96">
        <property type="entry name" value="OS05G0393800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="572"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="7"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="198"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="10">
        <location>
            <begin position="371"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="730"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="733"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK05559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0064180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="767" mass="87580" checksum="F00A7072670F5F7C" modified="2011-05-31" version="1">MGSLNEIGVAAGINISSALGFLLAFAILRIQPINDRVYFPKWYLKGTRSSPRHIGAGFSKFVNADLSTYLRFLNWMPAALQMPEPELIEHAGLDAAVYVRIYLLGLKIFVPIALLAFIVLVPVNWTSGTLEHEKDLNYDEIDKLSISNLGKGSKWFWAHIGMSYVFTFWTFYVLFHEYKVITTMRLRFLANQSRRPDQFTVLVRNVPPDPDETVSEHVEHFFAVNHRDHYLSHQIVYNANALAGLVEKKKGLKNWLVYYENQHAHNPAKTPTMKTGLWGLWGRKVDAIEYYKAAIEELCKQEDEERQKVMSDPNAIMPAAFVSFKSQWGAAVCAQTQQTSNPTVWLTEWAPEPRDVYWPNLAIPFVELSVRRLIIAVALFFLTFFFMIPIAFVQSLANLDEIERLLPFLKPIIERNSLKSVIQGFLPGIALKIFLILLPMFLMTMSKLEGHISISGLDRRTASTYFMFLFVNVFLGSVITGTAFQQLDTFIHQPANKIPETVGESIPMKATFFITYVMVDGWAGIAAEVLRLKPLVMFHIKNAFLVRTEQDREQAMDPGSLDFGTTEPRIQLYFLLGLVYAVVTPILLPFIIVFFSLAYLVFRHQIINVYNQQYESGALFWPDVQTRLIAALIVSQILLLGLLSTQEAEKSTVALLPLPVLTIWFHYVCKCRFEPAYVKFPLQEAMVKDTLQRANDPTLSLREYLKDAYVHPVFQKDDMYELVAMDEEEKNPTVATKRQSRMNTPVESKFNSSVGTNEGEFSRMHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F2EEP5</accession>
    <name>F2EEP5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123452231</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Seed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="AK374621">
        <property type="protein sequence ID" value="BAK05817.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984783.1">
        <property type="nucleotide sequence ID" value="XM_045128848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EEP5"/>
    <dbReference type="SMR" id="F2EEP5"/>
    <dbReference type="PaxDb" id="4513-MLOC_69401.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0093310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0093310"/>
    </dbReference>
    <dbReference type="GeneID" id="123452231"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0076850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0076850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0076850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0093310.1">
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        <property type="component" value="Chromosome 1H"/>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
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    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
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        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
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    </dbReference>
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        <property type="entry name" value="PPM_1"/>
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    </dbReference>
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        <property type="entry name" value="PPM_2"/>
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    </dbReference>
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    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="229"/>
            <end position="475"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="72"/>
            <end position="114"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="137"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="159"/>
            <end position="168"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="RuleBase" id="RU003465"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51746"/>
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            <dbReference type="EMBL" id="BAK05817.1"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0093310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="495" mass="53822" checksum="E9F8FA08934DA6F0" modified="2011-05-31" version="1">MVHDGAVKDHHHQQQREEQQEEESSPDIPATSASAVVAEAGAGVVGAAGTSKPGSDKRLGVRHPVKYRRFRVKGKAMAEPGVTPAHQPLEEELEEGEGEVEEEASSPEREVRAGVVEVEVEMEVSSSSAAAVEMEVEEGADMDVSPAAAVAMVDSEHSSEEDDDEEEVSSPVLALPDQARGRLVATLVVPKDQDLEKEKKEKERQRERERADEVGYMSGGCKSDDGNLSCGYSSFRGKRASMEDFFDIKSSKIDDKQINLFGIFDGHGGSRAAEYLKEHLFENLMKHPQFMSDTKLAISETYKKTDSDFLESEINTHRDDGSTASTAVLLGNHLYVANVGDSRAVISKSGKAIALSDDHKPNRSDERKRIESAGGIVMWAGTWRVGGVLAMSRAFGNRLLKQFVVAEPEIQEQEIDDELEFLILASDGLWDVVPNEDAVSLVKMEEDPEAAARKLTETAFGRGSGDNITCVVVKFQHSKTGSGDSPPISPPGDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2EF80</accession>
    <name>F2EF80_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123407761</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Seed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK374806">
        <property type="protein sequence ID" value="BAK06002.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045100963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EF80"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730400"/>
    </dbReference>
    <dbReference type="GeneID" id="123407761"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0606100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0606100"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730400"/>
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    <dbReference type="KEGG" id="hvg:123407761"/>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051949">
        <property type="entry name" value="Cation_Transport_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43079:SF1">
        <property type="entry name" value="CADMIUM_ZINC-TRANSPORTING ATPASE HMA1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43079">
        <property type="entry name" value="PROBABLE CADMIUM/ZINC-TRANSPORTING ATPASE HMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="745"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="257"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="70"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK06002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="828" mass="88150" checksum="A5E971C263437568" modified="2011-05-31" version="1">MQFLTTSAACSSSAPPLPRPAHLLRVSRPPPFPHLRRRRAPHPPSVSLSLAPKPSLLAASRRSLLFTPRAHGDHHHHHHHHHHGHGHGHGHHGHGDDGVEVRGGGGGAAVMRMARTIGWADVADALREHLQLCCISLGLLLTAAVCPHVPLLNSVGRLPAALIAIAFPLVGVSAALDALVDIADGKINIHVLMALAAFASIFMGNSLEGGLLLAMFNLAHIAEEYFTSKSMYDVRELKENHPEFALLLETSGDESAHFSNLNYAKVPVHDLEVGSHILVRAGEAVPVDGEVYQGSSTITIEHLTGETKPVERTVGDAIPGGARNLEGMMIVKVTKSWEDSTLNRIVQLTEEGQLNKPKLQRWLDEFGEHYSKVVVALSLAVALLGPFLFKWPFFGNSVCRGSIYRGLGLMVAASPCALAVAPLAYATAISSLASKGILLKGGHVLDALSSCQSIAFDKTGTLTTGKLMCKAIEPIHGHLDASNGVDPSCCTPNCESEALAVAAAMEKGTTHPIGRAVLKHSVGRDLPVVAVESFESLPGRGVVATLSGIKARDNESEFAKASIGSVEYISSLYRSYGESEQIKEAVKCSAFGPEFVQAALSVDKKVTLFHFEDEPRTGVCEVIYTLREKAKLRIMMLTGDHESSAQRVAKAVCIEEVHFSLKPEDKLNKVKAVSREGGGGLIMVGDGINDAPALAAATVGIVLAQRASATAVAVADVLLLQDNLCVVPFCIAKARQTTSLVKQSVALALTCIVFAALPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNPPTWSWADDIRQLVHSLKNYVSAKLNSSSSDCSASTVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F2EHA5</accession>
    <name>F2EHA5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123399115</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Flower</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375532">
        <property type="protein sequence ID" value="BAK06727.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949472.1">
        <property type="nucleotide sequence ID" value="XM_045093537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EHA5"/>
    <dbReference type="SMR" id="F2EHA5"/>
    <dbReference type="PaxDb" id="4513-MLOC_4430.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0467500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0467500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0467500"/>
    </dbReference>
    <dbReference type="GeneID" id="123399115"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0387010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0387010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0387010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0467500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0467500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0467500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399115"/>
    <dbReference type="eggNOG" id="KOG1919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="428658at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EHA5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF81">
        <property type="entry name" value="21S RRNA PSEUDOURIDINE(2819) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090964" evidence="7">
        <location>
            <begin position="18"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="8">
        <location>
            <begin position="192"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK06727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0467500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="472" mass="52012" checksum="FCBF659859A6C0F8" modified="2011-05-31" version="1">MTTRMAALLLLRRLAAGGAVARLQQPYAGLATAASRQDALDATREESGQPDKGNKNRWMELPPFAPVDAAAAARAIFRGQGGDEIEGSSNSTAIRWVRRCCPDLPASLVQKLFRLRKVKKNAVTAETSSTHASAEQFRLRRVSAKDHLLPGDTLFLPVNIQESSVAEKARKFDNRNEIDFLRSLEIYKDKAIIVVNKPPGMPVQGGVGIKNSIDVLAPMFEENSSDAPRLVHRLDRDCSGVLVLGRTQLSASILHAIFREKTADALADGTQQVLQRKYVALVMGTPRHPKGLLSAPLAKVLLQDGKSERLTVRAGPNTTSVQDALTEYRVIESCPQGFTWLELFPLTGRKHQLRVHCAEVLGTPIVGDYKYGRQAHQDWTPLPVSQTVDKELLRKQRLPFGLVLGGGSIAEQQPQLHLHCKQMMLPDISAAVQGLQSEDADRDFSDLEKLSFVAPLPLHMRLSWEVLKSVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>M0V0W9</accession>
    <name>M0V0W9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0V0W9"/>
    <dbReference type="SMR" id="M0V0W9"/>
    <dbReference type="PaxDb" id="4513-MLOC_16591.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0729940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0729940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0729940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0605560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0605560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0605560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0729940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0729940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0729940"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0V0W9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002651">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46662">
        <property type="entry name" value="DI-GLUCOSE BINDING PROTEIN WITH LEUCINE-RICH REPEAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46662:SF110">
        <property type="entry name" value="OS11G0233000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="12">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="215"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0729940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="726" mass="78516" checksum="E5DFCB99A4BFDDE6" modified="2013-04-03" version="1">MQELPFSPRNREGLSMPFLGLAALLLLFFASPTSSCTEQENNSLINFLDGLMPDGNGGLNVSWVKGTDCCKWEGIICSSDGTVTDVLLASRGLKGGISPSLGNLTGLLHLNLSQNSLESSLPTELVFSRSIIGLDVSFNRLKGHLQEMQSSNPSLPLQVLNISSNFFTGQFPRTAWDVTKNLVALNASNNSFTGQIPSSICINAPSFAMLDLSYNQFSGNISLGLGSCSMLRVLKVGHNNLTGALPDGLFNATSLEHLSLANNGLQGVLDGSHIVKLSSLTVLDLGSTGLSGKIPASVVQLRRLKKLYLEHNNMSGELPSALGNCSNLGYITLRNNSFTGDLSKVNFTMLDLRTADFSLNNFTGTIPESIYSCTNLVALRLAFNKFHGQFSPSIGNLRSLSFFSVTGNSFTNITNTLQMLKRCNNLTSLMMGTNFRGETIPHDETFDGFENLQVLTIDDCSLTGQIPLWISKLAKLQMLDLSLNQLTGQIPSWIDGLGFLFFLDISNNKLTGDIPAALTKMPMLLSERNATKLDTRFLELPVFWTPSRQYRTVGAFPSKLCLDNNNFTGAIPPEIGQLKMLDILNLSSNSLTGEIPQEICNLTNLQILDLSNNQLTGAIPSALNGLHFLSRFDVSNNKLEGEVPSGGQFDSFSNSSYSGNPKLCGPMINNDCNSGPSSAPGKRRNNLRRALVIGITAGGLIALILLACFLIARLAYDDHTENVVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>M0WI89</accession>
    <name>M0WI89_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123401545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951307.1">
        <property type="nucleotide sequence ID" value="XM_045095372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WI89"/>
    <dbReference type="FunCoup" id="M0WI89">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WI89"/>
    <dbReference type="PaxDb" id="4513-MLOC_51757.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551870"/>
    </dbReference>
    <dbReference type="GeneID" id="123401545"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0458560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0458560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0458560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0551870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401545"/>
    <dbReference type="eggNOG" id="ENOG502S4CH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WI89"/>
    <dbReference type="OMA" id="QMREMDL"/>
    <dbReference type="OrthoDB" id="1928656at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WI89">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="109" mass="11788" checksum="FB5D128B7E4D17A1" modified="2013-04-03" version="1">MAGQSPNQVSSAEAILVGALSSGVNAPTWIVLQITFLLLAFCFTAMLYLAFFSSDFVIVGHVLLLITIGAVLFVLLNRFLAETGFVPVEQQMQEMGIHKPEAAEKDKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>M0WYB9</accession>
    <name>M0WYB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="M0WYB9"/>
    <dbReference type="SMR" id="M0WYB9"/>
    <dbReference type="PaxDb" id="4513-MLOC_55613.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0705380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0705380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0705380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0585070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0585070.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0585070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0705380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0705380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0705380"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WYB9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000637">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001643">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF375">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0705380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="79533" checksum="7BBAB9446B6DEBC3" modified="2013-04-03" version="1">MKLNPEHPTEHWRAQPPIASPPTISLSLPTSFAAAAAVSCDGEGGGRLMRPSPTTAAAAASLSRQQLLRIQRCLPPVWRNAAPAQEPPPPSSFSPASLSSILFSCAAHRALRPGEQAHARAVTLGLAAHPSVLPRIASFYIAIGDPPAARSAVEQAAGRARAFPWNLLIWSYAGDRLWKDVVMAYDRMLAVGVDADRFTYPSLLRACGELGEVNIGRKVNHRIRRSRYDLDIYVWNALVGMYVRCGELEDARRVFDEMSVRDVVSWNTMVSGYASAGLWAEAFELLRRVPGANVVTWNAVSAGNLKAGNYDEVIRLLSQMRNHHGPGVDYVSLLIGLKACAKSGHPRVGRELHGVAVRLYFDRLECMVNSLITMYSRCRMMGSAELLFRMCSVRSITAWNSLLAGFAFMDQVEEASLLFREMIGSGVCPNDVTVLTMLSLVARFGNFCHGRELHCYILRHGQGGSKLLQNSLVAMYSKSRNMRASRIAFDQMECRDKHAYTSLILGYGMQREGHVSLKLFDEMIANNMEPDHVTMVAVLSACSYSGLVTQGQLLFTKMFAVFGIAPRVEHFSCMVDLYSREGLLKVSEEIIDKMPFQPTAAMLATLIEACLIHGNTEIGKRTAKKLLAMRTNNPGHYKLISNTYISAKCWPELAKVRSLMSMMDLTMVPSHSLLEPEYDICLVEQDDVLNHGAYYDLSDRTTDTDSSSSEEVKSSEAFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>M0X794</accession>
    <name>M0X794_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123447823</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasmic vesicle</location>
            <location evidence="10">COPII-coated vesicle membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980444.1">
        <property type="nucleotide sequence ID" value="XM_045124509.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980445.1">
        <property type="nucleotide sequence ID" value="XM_045124510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0X794"/>
    <dbReference type="SMR" id="M0X794"/>
    <dbReference type="FunCoup" id="M0X794">
        <property type="interactions" value="1998"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0X794"/>
    <dbReference type="PaxDb" id="4513-MLOC_57586.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343780.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0343780.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0343780"/>
    </dbReference>
    <dbReference type="GeneID" id="123447823"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0286950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0286950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0286950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0343780.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0343780.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0343780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447823"/>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0X794"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0X794">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000005">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000014">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF2">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23 C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="12">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="13">
        <location>
            <begin position="123"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="15">
        <location>
            <begin position="399"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="14">
        <location>
            <begin position="518"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="11">
        <location>
            <begin position="631"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343780.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="761" mass="83915" checksum="A637C80895C20F54" modified="2013-04-03" version="1">MSEFLELEARDGVRMTWNVIPGTKQDAASCVVPVSAMYTPLNPNPAIPVLPYAPLRCRICRAILNPFSVADFNSKMWLCPFCFQRNHFPQQYSTVSQSNLPTELYPECCTVEYMATAETGPVSPPVFLFVVDTCMIEEEIGYLKSALAQAVELLPDQSLVGFITFGTYVQVHELGFGLLPKSHVFKGTKEIKKDQILEQMGFFAGKTKPGVIAGARDGVSAESIARFLLPASECEFILNSLIEELQKDPWPVSADQRASRCTGAALSVAASLLGICVPGSGGRIMAFIGGPSTEGPGSIISKPLSDPIRSHKDLDKGSAPLYNKAVKFYEEIGNQLVHQGHVLDLFACALDQVGVAEMKVAVERTGGIVVLAESFGHSVFKDSLRRIFQSSDSDLGLSFNGIFEINCSKDVKVQGIIGPCTSLEKKGPLSSDTVVGQGNTSAWKMCGLDRKTSLCLVFDMAKKDAPDAIGQSQNNLFYFQFLTYYQHHDGQMRLRATTLSRRWVAGSGSVQELITGFDQEAAAAVMARLVSFKMEAEVDFDPVRWLDRALISLCSKFGDYQKEAPSSFSLSPRLSIFPQFIFNLRRSQFIQVFNNSPDETAYFRMMLNRENVANAVVMIQPSLISYSFQSGPEPVLLDVSAIAGDRILLLDSYFTVVIFHGITIAQWRKAGYQHQEGHEVFAQLLQAPQEEADSIIKERFPVPRLVVCDQYGSQARFLLAKLNPSVTYDSDNPPPPGGDMIFTDDASFQVFMEHLQRLAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287NQU3</accession>
    <name>A0A287NQU3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123448381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044981172.1">
        <property type="nucleotide sequence ID" value="XM_045125237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287NQU3"/>
    <dbReference type="SMR" id="A0A287NQU3"/>
    <dbReference type="FunCoup" id="A0A287NQU3">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287NQU3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362140"/>
    </dbReference>
    <dbReference type="GeneID" id="123448381"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0301750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0301750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0301750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0362140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448381"/>
    <dbReference type="InParanoid" id="A0A287NQU3"/>
    <dbReference type="OrthoDB" id="408413at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287NQU3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044233">
        <property type="entry name" value="CYP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47511">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47511:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019059" description="Peptidyl-prolyl cis-trans isomerase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="26622" checksum="60FC34AE5859A1AC" modified="2017-11-22" version="1">MEENRKMAGLFRHIAAALLFLVAAASAAGASTYYASDPNLGSARVVFQLNHGDIEFGFFPHVAPKTVEHIFKLVQLGCYNTNHFFRVDKGFVAQVADVMGGRKARMNKEQEQQAEKSIVGEFSTFKHVRGILSMGRYSDPDSASSSFSILLGDAPHLDGQYAVFGRVTKGDDTLRKLERLPTHKEGIFVMPIERIEILSTYYYDIDVESCEAEKSILKRRLSESASEVERWRRKCFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGP4</accession>
    <name>A0A8I6WGP4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123444202</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044976815.1">
        <property type="nucleotide sequence ID" value="XM_045120880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WGP4"/>
    <dbReference type="SMR" id="A0A8I6WGP4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005750"/>
    </dbReference>
    <dbReference type="GeneID" id="123444202"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0004360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0004360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0004360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444202"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF15">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="323"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="384"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="409"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="449" mass="48229" checksum="E4F2D266E0DC654F" modified="2022-05-25" version="1">MGHGVSCARTGDEHDFFRAAQLGDLDALGALLAADASLARRATLYDRLSPLHIAAANGRLEALSMFLDRGALPDAVDRHKQTPLMLAAMHGKIGCVLKLLHAGANILMFDSVHARTCLHHAAYYGHVDCLDAILSTARTTPVADSWGFARFVNVRDDHGATPLHLAARQGRPGSVQLLLENGAIVSALTGSYGFPGSTPLHLAARSGSLDCVRKLLAWGADRLQRDSAGRIAYVVAQKRHHGACAALLNPSSAEPMVWPSPLKFISELDPEAKALLEAALTEANREREKKILKDAKCSPQSPLQYDDNIDDDMFSEVSDTELCCICFDQACTIEVEDCGHQMCAPCTLALCCHNKPNPATLTMPSPACPFCRGSISRLVVAQTRADNADPDRPASPQLAHRRSRRSHNLSEGSSSFKGLSSAISKITRGSSRMADSESAAAMDKPEHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WH54</accession>
    <name>A0A8I6WH54_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WH54"/>
    <dbReference type="SMR" id="A0A8I6WH54"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0013380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013380"/>
    </dbReference>
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        </source>
    </evidence>
    <sequence length="1088" mass="122263" checksum="D5D65096DE4BA7E0" modified="2022-05-25" version="1">MMAKGFLAKSDNAGTKKSPLQIQMLESFYSEVQYPKPEDVTEYAASVGLTHNQVRIWFKERRRKERRHMEAAEVHMETQASARSNWPRCSSSRSSNSSQSPMQDTTGHHSYQDQSVLKKRKIMSPTARRSTLPFENNDPMRRHGKGKGLMTVWHAMYSQTAEIQDCSSFIDESGCLRSLRPFEDCDRKLAQVQRQTLPRKKVNKKSRPPPSKRKVPCGRVTDLTEHPPVECHLSVDESESSELRTEQATLVDDEELELSELQAGPNPLRCSAHLSSTGRHGCPLCKDLLARFPPPGVRMKQPFPTKPWESSPEMVKKLFQVVRFVYTHFGSMDVHPFTFDEFAQAFHDKDSSLLGKVHVSLLKLLMLNTERGSGSVFVPRSSKDSRFLSFLNFVREQEFDVNFWIKSLNSLTWVEILRQVLVASGFGSDHHILNRNFFNKEKNQMVKYGLRPRTLKGELFELLSKKGSGGLKVAELAKSPQIIDLNLSGASEVEQLIFSTLSGDITLFEKIAPSAYRLRVDPRIKGKEDPRSDTEDSGTVDDGGDASSSGDESDGPQESYPEHESRIVRWKQKNIHKNMNKCSEIDESYSGERWLLGLMEGEYSDLSIDEKLDCLVALIDVVSGAGSVPRLEEPQSVLSNIQRAQSHASGGKIKKSTRTIYQSSDEYLNRPGSSHSFDSSMQGQSGSLRGQDYIADSGANESPTRFAHQPQIVLLGSDRRYNNYWLFLGPCRADDPGHRRVYFESSEDGHWEVIDSPQDLLSLLSVLDIRGTREAHLLASMKKRQSCLFEGMKKHLEDGCVVALTASSDSSRSETSSGNRYSPKPSSGDGASPLSDIDSASVPTYLAGNLQNASSAIGIEVGRRSDEKMSKWERLQALDKWIWTSFYSSLTAVKCGKRSFKESLVHCESCHDLYWRDERHCRICHSTFEVGFDLEERYAIHVATCREPEDLYDVPNHKVLPSQLQALKAAIHAIEARMPTAAFAGLWMKSSHNLWVKRLRRTSSLPELLQVLVDFVGAIDEDWLYQSSSAVSFSSYLDDIIVYFQTMPQTTSAVALWVVKLDALIAPHLAQADSGRGLGKGSIETCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMG2</accession>
    <name>A0A8I6WMG2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Terpene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123431349</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMG2"/>
    <dbReference type="SMR" id="A0A8I6WMG2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0096330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0096330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0096330"/>
    </dbReference>
    <dbReference type="OMA" id="TTAINRW"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF230">
        <property type="entry name" value="TERPENE SYNTHASE TPS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="5">
        <location>
            <begin position="256"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0096330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="63115" checksum="6478BEA2BCA442C6" modified="2022-05-25" version="1">MQMATSNSIHPLVQDDVHKPRPYPASQWGDFFLDYKPCTPQQYLSMEGLAEAKKEEVRQIIIDTAKSSDLPQKLELVHTLQRIGVDYHYGKEIDELLCDIYDGNIELLDLRTTSLQFYLLRKHGYRVSSDVFSKFIDKDGNIGSTDAASLLGLYNAAYLRTHGEKILDVAMSSAKKILKSIVNHLDPTIADEVRHNLETPLFRGTHRVETKRYISAYEKDSTRNEIVLEFAKLDYNLVQGLYCDELKDLTIWWNGFDIETHLTWARNRMVEIHFWMMGVLFEPHYSYPRIVLTKLFTLVSVFDDFYDNYSTTEESNMFTTAINRWDEHAAEQAPTYMRPFYKGTVASVNQIEADLKLQKNKHAELVTKLFIDAANCYHAEVKWRDQNYVPTNLEEHLKISAPSTICMQISNIAFILMGDVTSSETIQWAWAYPTIIRAVCIIARVMNDIVSHEREQASQHMVSTVQTCMNENGCTLEEANEKLNEVVEQAWMDICESCIQPTVYPLAVLSRVANLARVTDFLYKHDDGYTLGYSVKGTLDSVYVHPLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WN32</accession>
    <name>A0A8I6WN32_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123426055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965766.1">
        <property type="nucleotide sequence ID" value="XM_045109831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WN32"/>
    <dbReference type="SMR" id="A0A8I6WN32"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0145810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0145810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0145810"/>
    </dbReference>
    <dbReference type="GeneID" id="123426055"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0120150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0120150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0120150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0145810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0145810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0145810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426055"/>
    <dbReference type="OrthoDB" id="10034502at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013744">
        <property type="entry name" value="SidJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591:SF1">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08538">
        <property type="entry name" value="DUF1749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0145810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="37536" checksum="AD056CCD76FB480D" modified="2022-05-25" version="1">MTSPASTSSWFSGIVGTASGNPLSSAMPPAGGVTSAPVSLPDTPAAVTSAPVATTGVCARRKQLQGTLFKYGPKSAQVAFRTGDFTRQLIFIGGLTDGLLATDYLEPLSLALEVEKWSLVQPLLSSSYIGYGISSLEQDALELDQLIGYLINKENSEGVILLGHSTGCQDIIHYMRTNFACSKAVSGVILQAPVSDREYRATFPETGEMIDLAAKMISTGRGMDLMPREANSDAPITAYRFHSLCAYMGDDDMFSSDLSEDQLKQRLGHMSTTQCLVIFSMADEYVPEYVDKKALVDRLCHALGDSEKVEIKWGNHALSNRVQEVVEVILDFVKREGPKGWDDPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNH1</accession>
    <name>A0A8I6WNH1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNH1"/>
    <dbReference type="SMR" id="A0A8I6WNH1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15828" checksum="90BBE4BA3B36379F" modified="2022-05-25" version="1">MVLVSASVGPRPVYDSDVEVCEPEIPTHIEILVEKLNLVEKENKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAIGSIVTLVAILIAFFVLFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR66</accession>
    <name>A0A8I6WR66_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR66"/>
    <dbReference type="SMR" id="A0A8I6WR66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0013180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0013180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0013180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0017100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0017100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0017100"/>
    </dbReference>
    <dbReference type="OMA" id="YIDFICE"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF552">
        <property type="entry name" value="OS05G0133401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240078" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0017100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="39638" checksum="7AFF612A02494F8F" modified="2022-05-25" version="1">MVRMLRGLVILAAVVVALHCCELPRRADGRCVLFNFGDSNSDTGSLPAAYGFYLGPPAGRRFFNRTTGRWSDGRLYIDFIAESLGIRYLSPYLESSGSNFTDGVNFAVAGAAAASNQSAIPFTMATQVNQFLHFKNRTRELRPLGQGSMLSEEDFLGAVYSIDVGQNDITLAFLANLTLQEIVADGGPLAAAAAKVEESVRALYGSGARKFWVYNTGPIGCLPQTLALRQRPGDELDAAGCLARYNAAARALNAGLAAACRRLADEYCGSATVVCTDMYAVKYDLFANHDRYGIERPLMACCGHGGPPYNYVNLKTCGQPTATACPEGERHVSWDGVHYTEDANAIVASKILSGDFSQPRTKLHALCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WRC9</accession>
    <name>A0A8I6WRC9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123443546</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044975897.1">
        <property type="nucleotide sequence ID" value="XM_045119962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WRC9"/>
    <dbReference type="SMR" id="A0A8I6WRC9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258150"/>
    </dbReference>
    <dbReference type="GeneID" id="123443546"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0214280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0214280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0214280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0258150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443546"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000166">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="13">
        <location>
            <begin position="20"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit C-terminal" evidence="14">
        <location>
            <begin position="671"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit appendage platform" evidence="15">
        <location>
            <begin position="815"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="950" mass="105374" checksum="00773B1964BA67E4" modified="2022-05-25" version="1">MDKPCTLLVHFDKGSAAMANEIKADLEGSDVAAKVEAMKRAVMLLLNGETLPTLFITVVRYVLPSEDHTIQKLLLLYLEIIDKRDAAGRVLPEMILICQNLRNNLQHPNEYIRGVTLRFLCRLSEPEVLEPLVPSILENLEHRHHFIRRHALSAISAIYRLPHGDQLIPDAPELVERALASEQDASARRNAFLMLCLCGQERAVAYLFSNSERVTEWPDLLQMAAVDLIRKVCRSPNRADKGRYIKIIISLLSSPSTAVVYECAGALVSLSSAPTAVRAAANNYCQLLTSQSDNNVRLILLDRLNELRTAHRDVMVDVVMDVLRALSSPNLDVKRKVLDLVLDLLTPRNVEEVVLYLKKEVVKTQSGELEKGGEYRQMLVQAIHACAVEYPEVAGSVVHLLMDFLGDTNVAAAVDVVLFVREIIETNPKLRVSMIQRLSDTFYQIRASRVCSIALWILGEYSLSLSEVESAITTIKHCLGDLPLFTLSEEGETADSSKPTQPMVNSVTVSSRRPVVLADGTYATQSAATEAISTPPVTAGSSASTLNLRSLILSGDFFLAAVVACTLTKLVLRLEEVQSSKIEANKACTGALLVMTSILQLGLSSYLPQPIDNDSYDRIVLCVRLLCNTGDDVRKIWLQSCRQSFAKMLAEKQFRETEEMKAKSQISHAQPDDLIDFYHLKSRRGMSQLELEDEVQDDLKAATGGFSKETDDANRLNRILQLTGFSDPVYAEAFVTVHHYDIVLDVTVINRTKETLQNLCLELATMGDLKLVDRPQNYTLAPESSKQIRANIKVSSTETGVIFGNIVYETSNVMERSVVVLNDIHIDIMDYISPATCADVTFRNMWAEFEWENKVAVNTVIQDEKEFLNHVIKSTNMKCLTPPSALDGECGFLAANLYAKSVFGEDALVNISIEKQVDGKLSGYIRIRSKTQGIALSLGDKITLKQKGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSD4</accession>
    <name>A0A8I6WSD4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Sucrose synthase</fullName>
            <ecNumber evidence="2 6">2.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123425299</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-diphosphate + sucrose + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:76533"/>
            <dbReference type="EC" id="2.4.1.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyltransferase 1 family. Plant sucrose synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.13" evidence="2 6"/>
    <dbReference type="RefSeq" id="XP_044964915.1">
        <property type="nucleotide sequence ID" value="XM_045108980.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964917.1">
        <property type="nucleotide sequence ID" value="XM_045108982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSD4"/>
    <dbReference type="SMR" id="A0A8I6WSD4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0124890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0124890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0124890"/>
    </dbReference>
    <dbReference type="GeneID" id="123425299"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0102950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0102950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0102950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0124890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0124890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0124890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425299"/>
    <dbReference type="OrthoDB" id="937291at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016157">
        <property type="term" value="F:sucrose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1230:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.330:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000004">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012820">
        <property type="entry name" value="Sucrose_synthase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056736">
        <property type="entry name" value="SUS_EPBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056735">
        <property type="entry name" value="SUS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02470">
        <property type="entry name" value="sucr_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839:SF5">
        <property type="entry name" value="SUCROSE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24862">
        <property type="entry name" value="SUS_EPBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24861">
        <property type="entry name" value="SUS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase EPBD" evidence="10">
        <location>
            <begin position="160"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="8">
        <location>
            <begin position="270"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="564"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0124890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="816" mass="92587" checksum="923764E780DC9CE6" modified="2022-05-25" version="1">MGETAGERALSRVHSVRERIGDSLSAHTNELVAVFSRLVNQGKGMLQPHQITAEYNAAIPEAEREKLKNTPFEDLLRGAQEAIVIPPWVALAIRPRPGVWEYVRVNVSELGVEELSVPEYLQFKEQLANGSTDNNFVLELDFGPFNASFPRPSLSKSIGNGVQFLNRHLSSKLFHDKESMYPLLNFLRAHNYKGMTMMLNDRIRSLGTLQGALRKAETHLSGLPADTPYSEFHHRFQELGLEKGWGDCAQRASETIHLLLDLLEAPDPSSLEKFLGTIPMVFNVVILSPHGYFAQANVLGYPDTGGQVVYILDQVRAMENEMLLRIKQQGLDITPKILIVTRMLPDAHGTTCGQRLEKVLGTEHTHILRVPFKTEDGIVRKWISRFEVWPYLEAYTDDVAHEIAGELQANPDLIIGNYSDGNLVACLLAHKLGVTHCTIAHALEKTKYPNSDLYWKKFEDHYHFSCQFTADLIAMNHADFIITSTFQEIAGNKDTVGQYESHMAFTMPGLYRVVHGIDVFDPKFNIVSPGADMSIYFPYTEQQKRLTSLHTEIEELLFSDVENAEHKFVLKDKKKPIIFSMARLDRVKNMTGLVEMYGRNPRLQELVNLVVVCGDHGKVSKDKEEQVEFKKMFDLIEKYNLSGHIRWISAQMNRVRNGELYRYICDMKGAFVQPAFYEAFGLTVIEAMTCGLPTFATAYGGPAEIIVNGVSGYHIDPYQNDKASALLVDFFGKCQEDPSHWNKISQGGLQRIEEKYTWKLYSERLMTLSGVYGFWKYVSNLDRRETRRYLEMLYALKYRKMAATVPLAVEGETSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WT61</accession>
    <name>A0A8I6WT61_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WT61"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0135780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF43">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="309"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="647" mass="69202" checksum="4738BEBF1A8EBFFA" modified="2022-05-25" version="1">MDAHTGAPLTPLYSRDPPAAAAAAADAAAAAAENPSAGAAAATGAPPTVGLARSLFLPPRMTTPTGGVASAPSRAAAAPSKLPNASEPKKGKTSAKKKAADGSGSSKAKKKLAGRRTGAASTEAPMSSLFEPAADAHHVFDEMLPSLNDDAYMSTMGVGSNNSHWSQTNDIHLDDHEFEVDEEGEGIVEAPKGRAGNYTTNDDKLLCNTWLQVSRDPSIGGDQSRDAYWGRMKEYFDAQNVSGIDRSERSLRSRWSTINSDCQKWTAAQKAVDKLNPSGTNEDDRYNIAQKLFKEETRTTKKGKIKKGKIFTLPHCYEVLKDDEKWKKREDLDDLHLSNKRKRTIELNDDEEEDDASSDDGKRSHTPNSVSYSKPKRPDGCKKDKTEKKKRKGDDELTNAMEAIVKARKEANEVRKMARNQDAAAEERRLAAEERRVAAEERKVALEERKVGMKERAKLLEWEKHLFFLDTSLFNDAQKKYVNLAREEVLIQKRAMIRGGLGGMGGMGLGAMGGLGGFGAMGGMGAPPAAMGGMGGFGAPSNAMGGMGGFGAPSNAMGGMGGMSFASLTGGMGVPLVVMGGMGAPPAMGGMSFDVPHTHTHENAVEELAKTVGATRDAVRDEVREEDSSSKAEESSAEEEEEDEEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WV22</accession>
    <name>A0A8I6WV22_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WV22"/>
    <dbReference type="SMR" id="A0A8I6WV22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0074200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0074200.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0074200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF912">
        <property type="entry name" value="NB-ARC DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="109"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="229"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="390"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="70065" checksum="AD3E638E694FB700" modified="2022-05-25" version="1">MEPLDDVDSRRLFFKRIFRMDDPCPVELEEISTKILQKCGGLPLAIITFASLLANKTHKKEEWERLQESIGTGPSFDSDGNLKGMKDILLLSYWDLPHHLKTCLLYLCIYPEDSMIDTKLLKWKWIAEGFIASQWGSLDQVAENCFNELVNRNMIQPVYGNDYSSVKYCRVHDMVLDLIISLSDEENFATVLNGRICNSFPNNIRRLSIQSSGKEDKGAVGAITETKTHVRSLTVFGLHNQIPQLVDFHALRVLDLAGRYFLENKHVKHIGSSCQLRYLRIDSFRMTELQIGKLQHLETLDLRDCSSLPRLPSTVARLRKLVRLFVSNDTQLAAAGFRSLQALEELSFQETDDPVRFAEEVNELGKCNLRYLCTGGEIAERLFCNPCCTYTCLQDLQIQQAVGMVPRGMASLKNVVKLDMAVMEFDNEGLQVLMGLPSLAHLQLHVIRGVKEKLTIGSDGFRLLKFFEFQHLSYFHFVLEEEPTSHQVRLTFAPGAVPALRLLRLNLSPMRVASDFFADFGVEHLSVVAHLEVVINCISVVPGWVKALECSVAKAIKLNPDHEIRVIRVLEDHMFKDEKEWEEYISKMKSTSSSKKKGFACVGETDHPKLASVILKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WV66</accession>
    <name>A0A8I6WV66_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123413579</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044962451.1">
        <property type="nucleotide sequence ID" value="XM_045106516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WV66"/>
    <dbReference type="SMR" id="A0A8I6WV66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069530"/>
    </dbReference>
    <dbReference type="GeneID" id="123413579"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0055850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0055850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0055850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413579"/>
    <dbReference type="OMA" id="KGDCLQW"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000138">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1395">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="453" mass="49237" checksum="2084DD6B03A82C05" modified="2022-05-25" version="1">MGSVAVPPCHVVAVPFPGRGHINSMLNLCRLLAARDGVSATVVVTEEWLGLLGGPVHAPGVRLETIPNVIPSEHGRAADWAGFLDAVYAEMELPFEHLLDHLGAAPAAILADTFVPWAVRVGNRRGVPVCVLSALGATMFSVQYHFDRLPTSAGGSADIADATDPCLIENYIPGLRSIRLTDLEPTHSDKMRLGKILEAYPYVRKAQCIIFTSFYELESNAIEFLRQELPCPVFAVGPCIPFMALQENHADSGEEQGYMAWLDAQPASSVLYVSLGSFLSVSSTQLDEIAIGLAQTKVRFLWVLRDEVARVQHLIRGSDGVVVPWCDQLKVLCHPSIGGFFTHCGMNSTLEALYAGVPMMTLPIAFDQPVNSRLIVDEWMVGYGLKEKARGDGVIARDKISEAVKRLMDCGDGEGTRRRASLLKEASRAAVEVGGSSDRDMASFISYISHFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WY74</accession>
    <name>A0A8I6WY74_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123451036</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983987.1">
        <property type="nucleotide sequence ID" value="XM_045128052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WY74"/>
    <dbReference type="SMR" id="A0A8I6WY74"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0336080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0336080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0336080"/>
    </dbReference>
    <dbReference type="GeneID" id="123451036"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0280260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0280260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0280260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0336080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0336080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0336080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451036"/>
    <dbReference type="OMA" id="PRGKNFC"/>
    <dbReference type="OrthoDB" id="585946at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000023">
        <property type="entry name" value="Senescence-specific cysteine protease SAG39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035250432" evidence="9">
        <location>
            <begin position="25"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="37"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="123"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0336080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="341" mass="36819" checksum="D00B9F33BD5BDBA8" modified="2022-05-25" version="1">MAIPKALLLAILGCICLCSSTVLSARDLGDAAMVERHEQWMAKFNRVYKDETEKAQRFEVFKANVAFIESFNAGYHKFWLGVNQFTDLTNDEFRATKTNKGLKRNGARAPTGFKYNNVSTDALPAAVDWRTKGVVTPIKDQGQCGCCWAFSAVAATEGIVMLSTGKLISLSEQELVDCDVHGVDQGCEGGEMDDAFKFIIQNGGLTTEANYPYTAQDGQCKTSVASNSVATIKGYEDVPTNDESSLMKAVANQPVSVAVDGGDVIFQHYSGGVLTGSCGTDLDHGIAAIGYGMASDGTKYWLLKNSWGTTWGESGYLRMEKDISDKSGMCGLAMQPSYPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYM4</accession>
    <name>A0A8I6WYM4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123451109</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044984053.1">
        <property type="nucleotide sequence ID" value="XM_045128118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYM4"/>
    <dbReference type="SMR" id="A0A8I6WYM4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341310"/>
    </dbReference>
    <dbReference type="GeneID" id="123451109"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0284820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0284820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0284820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0341310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451109"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22586">
        <property type="entry name" value="Rcat_RBR_ARI1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16773">
        <property type="entry name" value="RING-HC_RBR_TRIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="127"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="542" mass="62102" checksum="B3640C50E7A95B87" modified="2022-05-25" version="1">MASDDDCYDYEYDYDEVEEEDDDDDEMMMMEADEDGLVEEDTPAPERPADCWAITQESLSTAQQQDLSMVMNLLNVKQHNARALLIHHRWKIDCIYDHLDRKGRDHMFREAGIVLQENINGKAPPSRLVNCMVCFDEFSVGAVSTMECGHYFCNDCWTEHFKASVESGKKQIRCMGVKCPVVCDEPVVQQLLARKYPDTARRFDRFLLESYLEGNDSVKWCPSVPHCGRAIRVGAGERYCEVECPCGLGFCFACAAGAHSPCPCTMWDMWEVKCNGESETVNWILANTKSCPKCFKPIVKDGGCNLVTCKCGQYLCWKCGAATGYEHTWDSIDGHSCNRFTGEEKKKVDTAKRNLDRYTHYYDRFKIHGDSYKLEHDKLGPAIEERAKQLEALHDQPLVRDCTWLMEAHRGLLRSRQVLLRSYVFAYYMFGSDDDDDKVRMRPADRDNLAVAQGLFENFQEELERHVERLSELLATEYGHKPEEESVLHDKQNAMNLAKIIQTNCGEIYTCIQDELLPLLLDPITIAAYRPRGPDKAKEFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0T4</accession>
    <name>A0A8I6X0T4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">La-related protein 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044977083.1">
        <property type="nucleotide sequence ID" value="XM_045121148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0T4"/>
    <dbReference type="SMR" id="A0A8I6X0T4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289780"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289780.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289780.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289780"/>
    </dbReference>
    <dbReference type="GeneID" id="123444440"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0240670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0240670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0240670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0289780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0289780.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289780.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444440"/>
    <dbReference type="OrthoDB" id="435402at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF159">
        <property type="entry name" value="LA-RELATED PROTEIN 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="7">
        <location>
            <begin position="161"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="261"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="357"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="50909" checksum="6A55384D6C743AB9" modified="2022-05-25" version="1">MDGQAPHPVAVAPKSPPQPPPRLDMEAEDDPVVVPDAADAASSEASKVGAHGVAPLLSTSSLLKMEDDPVVVPDTVEPASSEASEVGAGGVVLLQPPSLSLEAEEDPLIVLGTSETASLEATNVGTGVVVPSPPLEAGDDPLILPGTDASLEASDVLTTGVVLTDELRDKIVKQVEYYFSDENLPTDEFMLKFVMKNKEGLGNVPLGVIASFRKMKKLVQDHSAIEAALRTSSKLVVSSNGKRVRRLHPLPCNELKDVKRRTVVVENLPLDFSLESLQEKFGSVGKIMKITIHDPHAVGESAASKKPDFMLSNKVHAIVEYEAVEAAEKAVATLNDERNWRTGMRVILLAKRSVVGSGKHIQSSKENHGTVSRKNNEGQFSKEVQQSASEKNGGADSGEVASDKENVNCDVNHEEVRQHQRTNASGGRKGRYRSQGHGSSPTIPGSDPANKPISGPRMPDGTRGFTMGRGRSLPLPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X475</accession>
    <name>A0A8I6X475_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X475"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0244430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26322" checksum="7D1479C34C2C65AD" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHLSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGCLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVEWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4U2</accession>
    <name>A0A8I6X4U2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21307" checksum="A953AA1B1A7DB728" modified="2022-05-25" version="1">MEETSCCLTLKQHLNKSSFTVKVNPLKKISWKFQNLRVAHLTAIFGSLPSIWSIFSLLRFGPHVEELHIQADTPFAVFEGLFPAALDAVDLDDNIDQDVLDAKISDDLFANLKQISLEDMNCLPNDMWFMKVLLSKAGLLQSFVVTLDYGGETESYEEASAELEACERASPQAQLELRLAEMPDYLEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6R3</accession>
    <name>A0A8I6X6R3_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123445382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6R3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314810.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123445382"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="246" mass="27184" checksum="DED21FAE60A5FC28" modified="2022-05-25" version="1">MAGGGRSEKNGGGCGPCCLRFLLKLLAFPQAFAALSAVLYAASILSRWARHHELHIHHLLPDLWFACAVMAAGLLYCAILLAVYIAAEFSSGCCFYFYTGLASVMILLEEAVAHNILLNEHWIQYLPYDRTGELQNLVSFVNNNLDLCQWAALATFATQVLSLFLAMILRAWVSPTSSDYDNNEDFVVIRKPLLIAQGAPSYLPTTADPRGVHPNLWSSSMRQKYGLSSSGDYTYNTLDQNAVRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7G9</accession>
    <name>A0A8I6X7G9_HORVV</name>
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        <recommendedName>
            <fullName evidence="3 11">Cysteine synthase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7G9"/>
    <dbReference type="SMR" id="A0A8I6X7G9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0424290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0424290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0424290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0352570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0352570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0352570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0424290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0424290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0424290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000130">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="132"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="304"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0424290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="444" mass="46170" checksum="2DC9E6CF16DEB0AA" modified="2022-05-25" version="1">MMNAIGQPSPAASLKAATPSTHIRRRQTPSRFNERRPSGPPAAGPTRSVTVHRGQRLPAGPPSSGPNLLHQPRLYKASPPPPAPPPTTSTTGQPAAAATSSTSSSGRTASLPNRSSGGGMGEASTPAIARDVTELIGNTPLVYLNKVTDGCVGRVAAKLESMEPCSSVKDRIGYSMITDAEEKGFIVPGKSVLIEPTSGNTGIGLAFMAAAKGYRLVLTMPASMSMERRIILKAFGAELILTDPLLGMKGAVQKAEELAAKTPNSYILQQFENAANPKIHYETTGPEIWKGTGGKIDGLVSGIGTGGTITGSGKYLKEQNPNIKLYGVEPTESAVLNGGKPGPHKIQGIGAGFIPGVLDVDIIDETVQVSSDESIEMAKSLALKEGLLVGISSGAAAAAAIKVAQRPENAGKLFVVVFPSFGERYLSSVLFHSIKKEAESMVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEH3</accession>
    <name>A0A8I6XEH3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEH3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0363930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0363930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0363930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0363930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0363930.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0363930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0363930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28848" checksum="4E3B7CCD16884719" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYTCTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFFYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPAPQVELPGLGGNLYEMFSADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XET7</accession>
    <name>A0A8I6XET7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein VACUOLELESS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123444053</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for vacuole biogenesis and vacuole enlargment in dividing and expanding cells. Involved in the docking or fusion of prevacuolar vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976593.1">
        <property type="nucleotide sequence ID" value="XM_045120658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XET7"/>
    <dbReference type="SMR" id="A0A8I6XET7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0278400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0278400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0278400"/>
    </dbReference>
    <dbReference type="GeneID" id="123444053"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0230660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0230660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0230660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0278400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0278400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0278400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444053"/>
    <dbReference type="OMA" id="WCGDDCL"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.780:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="53"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="534"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0278400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="871" mass="95491" checksum="95133CF8609C7081" modified="2022-05-25" version="1">MSSSISVAAEWDLLSDRFYRRVTIYSPLPWSSPATTTASSSGGGSSGVGRLDLSTHIVAAAPFGGPIAAVRDDSKIVQLHSEPSRRRLLLFSSSGHPLASSPWTPHLPRLHSLAFSSSLNLLALLSDGSLLRFRLPDLNPITSSSPVPLLPPASGGVADAVFWGGGVAILTEDNRVVVTTDIEVDDPHPRELADPGVAEDEQVLCMAVVEPQFVMSGSPEVLLAVGDRVVAVDEDGVQVLGEALEIGPVQKMAVSPNGKLLAAFAHDGRLLVIPTDFSRIIFEYECDSALPPDQIAWCGLDSVLLYWSEVLLMVGPNGDPVQYNYDEPVMLIPECDGVRILSNSSMEFLHRVPDSTTSIFGIGSMSPAALLYDARDHYDKQSAKAYDNYQLISSSLPEAIEACIDAAGYEFDVSRQHTLLRAATYGLAFCSRFPHGRFQEMCKILRVLNAVRDPEIGMPLTVKQYKLLTAAVLVGRLINANQHLLALRISEYLNLNPEVVIMHWACEKIAASAAIPDVVLLEGLLDKLRLCKGISYAAVAAHADNSGRRKLAALLVDHESQSSKQIPLLLSIDEQDKALQKSIESGDTDLVYLVLFHIWQKISVEKSAPLEFFGVINARPLARDLFIAYARHSKHEALKDFFLSTGRLQDAGFLLLKESRELERSPMASKGSPLHGPQVRLVEQAHRLFAETKEHLFESKSAEEHGKLLRVQHQLEVSTKQAIFVGSSVSDTIKTCIVMGNERAAVKVKSEFKVPDKRWYWLKSCALATVGNWDALETFSREKRPPGGYKPFVEACIDAGQKMEAIKYIPKLTDPGERSEAYARLNMTDEAEKAASEANNGDELFGRLKSTLAQNTLIDTLRDRLSFQGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGR3</accession>
    <name>A0A8I6XGR3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123427772</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967822.1">
        <property type="nucleotide sequence ID" value="XM_045111887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGR3"/>
    <dbReference type="SMR" id="A0A8I6XGR3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194480"/>
    </dbReference>
    <dbReference type="GeneID" id="123427772"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427772"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04093">
        <property type="entry name" value="HBS1_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001277">
        <property type="entry name" value="Elongation factor 1 alpha-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000060">
        <property type="entry name" value="elongation factor 1-alpha isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000227">
        <property type="entry name" value="Translation elongation factor EF1A/initiation factor IF2gamma family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="246"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="96"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="681" mass="74205" checksum="283FA3F14A2B002E" modified="2022-05-25" version="1">MPRKVVSGPDYDDDYADYEDYEDDYDDDYDETGHADVKPPVKEKESLKNSSNAVPVLWKCSMCTFDNHETMVYCEMCGVFRESFVKSGKDGSIKVDSVNGISNNSGTSALSNSDSTKMPAKTSTTNFDGDSERKYASTSHDKVNSAQLASVGSSSSTGKKKQPIISDKDVPVERTPQLIADHFQLKEDQGSRVSCSAQNKGSMETLSSDISELSIERHNVNVAPPYLPEEYKPEGWMLADQESGVLSQLNLAIVGHVDSGKSTLSGRLLHLLGKISKRDMHKNEKEAKEKGKGSFAYAWAMDESTEERARGVTMTVAVAYLETKKYRVVLLDSPGHKDFVPNMISGATQADAAILVVDASTGSFESGMDGDGGKNVGQTKEHAQLIRSFGVEQLIVAVNKMDAIGYSKDRLEFIKVQLGSFLRSCNFRDSAVTWIPLSAVENQNLIKSPSDARFTSWYQGLCLLDAIDSLQLPSRDVSKPLILPICDVIKSQSTGQLAAFGKLETGAIRNGSKVLVLPCEQVATVKTIERDSSSCSTARAGDNVAVILQGIDGNRIIPGGILCHPGFPVPVANYLELKIRVLDITIPILVGYQVEFHIHHVKEAARVTKIVALLDKTGKPSKTAPRFLKSKQNAVVQVTLDQAVCVEEFSKCRALGRAFLRSSGSTIAVGIVTKIMRQDQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI43</accession>
    <name>A0A8I6XI43_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exopolygalacturonase</fullName>
            <ecNumber evidence="8">3.2.1.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Galacturan 1,4-alpha-galacturonidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Pectinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl](n) + H2O = alpha-D-galacturonate + [(1-&gt;4)-alpha-D-galacturonosyl](n-1)</text>
            <dbReference type="Rhea" id="RHEA:14117"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14572"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58658"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.2.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.67" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI43"/>
    <dbReference type="SMR" id="A0A8I6XI43"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0164140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0164140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0164140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0197670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197670"/>
    </dbReference>
    <dbReference type="OMA" id="SKCENAN"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047911">
        <property type="term" value="F:galacturan 1,4-alpha-galacturonidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035199666" description="Exopolygalacturonase" evidence="16">
        <location>
            <begin position="29"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="406" mass="43649" checksum="AE2290A57B04FA92" modified="2022-05-25" version="1">MEQRPRPVVVAVNALLCVVSLCVAAAGAENVTAVTTYDAKNYGATGNGVDDDTKGLMAAWKAACGTPGTVTLMIPPGTYYIGATQFHGPCKASAITFLLQGTLMAATDLKRFGNDWIEFGWVTDLTVAGQNGGIIDGQGAASWPFNKCPVRKDCRVLPTSVMFVNNENTVVRDITSVNSKFFHIALLHNNNMRMSNLRIHAPENSPNTDGIHIERNTGVIISDSHIGTGDDCISIGQGNQNIHIARVHCGPGHGMSVGSLGRYVGEGDVTGIHVTDMTFVGTMNGVRVKTWENSPTKSLAAHMLFENMVMKDVQNPIIIDQKYCPYYNCEHKYVSGVTLKDIKFKNIKGTSTLPVAVLLRCGVPCHGVVLQNVDLSYNGQGGTSSKCENANATYVGYQHPKPCDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XII4</accession>
    <name>A0A8I6XII4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123427972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">subsp. vulgare</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298131" description="Expansin-like EG45 domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="147"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="15493" checksum="E9B93C69730104D2" modified="2022-05-25" version="1">MAGTTKVAVVLGIALVFLQVSCAVSGRPTVAQGGKFEPRQNVPATMTVNGFQQGEPGGGPASCDGQFHSDDEFIVSLSSEWYAGGARCGKYIRIADYANLHITAKVVDECAGCDGEVGASIHIWNNFRLDTSVGQADIKWSDVPDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XL73</accession>
    <name>A0A8I6XL73_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late embryogenesis abundant protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044975682.1">
        <property type="nucleotide sequence ID" value="XM_045119747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XL73"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253630"/>
    </dbReference>
    <dbReference type="GeneID" id="123443402"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0210580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0210580.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0210580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0253630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443402"/>
    <dbReference type="OrthoDB" id="1936089at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="entry name" value="LEA_3a"/>
    </dbReference>
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        <property type="entry name" value="LATE EMBRYOGENIS ABUNDANT PROTEIN 2-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33509:SF5">
        <property type="entry name" value="PROTEIN SENESCENCE-ASSOCIATED GENE 21, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03242">
        <property type="entry name" value="LEA_3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="9478" checksum="FFE2C103BA11C2BD" modified="2022-05-25" version="1">MALALSGSAAARALAQRLAPATTRGYAAAASSGAMRRGAAATADGKAAREAEKAAADSSWVPDPVTGHYRPANRSSGADPADLRAAHLGQTYARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMH8</accession>
    <name>A0A8I6XMH8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMH8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0378070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0378070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0378070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0456820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="11732" checksum="DE6F2164B96E3C3F" modified="2022-05-25" version="1">MDLLQFVLNFKDTRPWLNAMALTALTDNWRPETHSFHLPLGEMTTTLEDIVMITGLLIEGMALTGKVRSDGWRQRVAALVGVEPEPWTHETRKDPRPSDVLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPG6</accession>
    <name>A0A8I6XPG6_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">GPI inositol-deacylase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC123449647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982869.1">
        <property type="nucleotide sequence ID" value="XM_045126934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPG6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406000"/>
    </dbReference>
    <dbReference type="GeneID" id="123449647"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0337970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0337970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0337970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0406000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0406000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0406000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449647"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48187">
        <property type="entry name" value="LD21810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48187:SF2">
        <property type="entry name" value="LD21810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="750"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0406000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1188" mass="129081" checksum="BA57761A8867231D" modified="2022-05-25" version="1">MLRRLLAASLRHARLRPRRLLSSASPSPPSASSSTLPVAAPPRPRHHLAPLHGPRRVSPLLAISALSVATAAGTLYLTTDNIEETLERSRASAARVAEQMRHTWTAGGVLCKSLASVLSSANHEVRSGFELRVAALLADITAASAARRAAIVSAGGGAVVDWLLDSIVRGATQAEAARALANLLADPWVAPAVLGRPRAVPSLLQFIFSYQPKRGHKNSRHSSFDVSDHSKGRSMLVAALMDIITSNCDKADYSSFQPLLPADADIRDIAAAIEVIEEGGMHFDDHEDDSSDDGDRGLKGIGIKVLGGTTILGFSRENNSLKIDNSGDDTLEVAQNSRMEVARNSSGLVNQEPTVDYVDIERLSSNATPGLWDDLQREHVAVPFATWALANWAIASDLNRTRIQELDSDGHAVTTALKAPERTVKWHGALVARALLEDQNLTLAPSVPDWSSSLLLTASQATENGDMSLAQMSLSTFLLSLIRCNESKFVIRQKGLHLLRSIAKKIHNENGQSGMKESLAVALSSLYSGEVPLSLEEAQRWSGILLRWLFDKSVSGTTHLTSVKILSSILEDYGPSSVPISQGWLALVLSEILGDNKTQSLKGTTPPEPERVKNQVDYHNAYTATQVLNQLATAVVKLASIQSDHDSGSGDKVPLYDFLSLEPFATALKNLNKKSPPKFDAVDSALATLKGIKALAELSSEDVACQKRIADLGVVSLLKHILVGDDYEKLAAIEAYDASRIREVQDKNVSASDDSSTAATTDPRSVRVPPAAHIRRHAGRLLTILSLLPNSKKEIVSDDVWCKWLEECATGRIPCNDIKLKSYCRLTLLNVFCSENQTTKSASGEYPDSECEYKRKCPQFGDALFLLNPELPLEVHLDKSGCGISRDSCKDDGCIEHSGSETGSIEGPDAASRRVNPEVDVVFIHGLRGGPFNSWRIADDKSSTTKAGLVESIDEDAGKEGTCWPRQWLSSDFPQARFLTVKYKTNLTQWTGASLSLQEVSSMLLRKLVAAGIGSRPVVFVTHSMGGLVVKQILHQAKLNNYDKFLNNTIGLVFYSCPHFGSKLADMPWRMGYVFRPAPSIGELRSGSPRLVELNDFVRQRHSKGLLDVLSFSESEVTPIVEGYGGWAFRAEIVPIESAYPGYGELVVLQNTDHINSCKPVNKNDPSYAETLAFLEKSLKSRGKRAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQJ0</accession>
    <name>A0A8I6XQJ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Negatively light-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448841</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981799.1">
        <property type="nucleotide sequence ID" value="XM_045125864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQJ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0382790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0382790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0382790"/>
    </dbReference>
    <dbReference type="GeneID" id="123448841"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0382790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0382790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0382790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448841"/>
    <dbReference type="OrthoDB" id="5949865at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34804">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 19-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34804:SF5">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 19-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0382790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15677" checksum="6EAC3FF92762F49B" modified="2022-05-25" version="1">MYVMSVVHQVFMVLTRATSIYIVSPLSGSHLTSLSLPPPPPRLLPTHTGYTRAATEEMAGCGEAKTSSVSMDEEASMERKFGGIAPKKPLISKDHERAYFDSADWVLGKQSANSSSSAAVESLKPKLKRTPHHQLPPRKPTCASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQQ0</accession>
    <name>A0A8I6XQQ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123404328</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044954182.1">
        <property type="nucleotide sequence ID" value="XM_045098247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQQ0"/>
    <dbReference type="SMR" id="A0A8I6XQQ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547660"/>
    </dbReference>
    <dbReference type="GeneID" id="123404328"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0455150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0455150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0455150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0547660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404328"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000168">
        <property type="entry name" value="Protein BONZAI 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000186">
        <property type="entry name" value="Protein BONZAI 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF130">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="28"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="174"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="334"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="64834" checksum="400082E62DA53136" modified="2022-05-25" version="1">MGGCFSGDVRGGTEPVGVGARAAAAGQGQGGPNEAVDHFLQGHGLRGLYTPLELSFSASKLRDMDALSKSDPMLVVYTKMDGRLEEIGRTEVILNSLEPLWITKTMLNYQFEIVQPLVFRIYDIDTKYHNTPVKMLNLAQQDFLGEAFCNLSEIVTKFNHSLTLNLRNGSGHALQGTMTVHTEETASSRTAVEMTFHCLNLDNKDTFSKSDPFLRISRLSESAVAIPICKTEVINNNLNPVWRPITLTSQQYSSRDDPLLVECFDFDASGDHELMGALQATIAQLENLYKSKAGANFYSHKGQKKLKGQLFLDTFLEKVQHTFLDYISSGFELNFMVAVDFTASNGDPRTPQSLHYIDPSGRLNSYQQAILEVSEVLQFYDKDRRFPAWGFGARIPQGSVSHCFNLNASTNDCEVVGFEGIMSAYSSTLYTVSLAGPTLFGPVINKAAEIASHSVQYVNNKYFVLLIITDGVITDEQETKDSIVRASDLPLSILIVGVGSADFTQMRTLDADLGKRLQSSTGRVATRDIVQFVPMREVQAGGQVTVVQSLLEELPGQFLEYMRTRGIKPRPPQHASASPAYPPPPPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRA7</accession>
    <name>A0A8I6XRA7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRA7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0470520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0470520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0470520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0470520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0470520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0470520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF47">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0470520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="547" mass="58588" checksum="6C94D8D64D000EEA" modified="2022-05-25" version="1">MLHHLDPARRPRESRSTTVPSTAVDQVVFFLRSHAVTLSVADGVNATSPMAVGKAMEAQLSVSVHSLRVTAHHPQHFFVIFTQPAHQVNAVRQGSIRVDGACFNIVSWHEHDHATFDSLLLHVRVVIEKVPMHFWSVEGAEEILGRRVRVDRLDSRTLEGGSHQDLRLLDRRVAPPPASAEYSMLIHVDRVEDWTSPFPRSSHSGQSGLPSSGSDDDATPFLVVAPASCAMGVEDGQRTDPLQSFARAPIANLGCRGAPRGGRSRDHDGDGAPGGGGHRSWKDVLLRRGSSQAPPRAAPSQRRRSRSPPARRRSRESWGRRSGAGRAPASARRQPKQHRAIPTTPTFAGETGSDAAAIQAGSVAKGNLDGKDPIEDFFRMAKRPSIASIIVDSMATDVHNATEAAFASPLEFDEAPLLHSSVDAVQLDAFSPTLSAAASDLGQFNRATPSSAHTMEVQLGAVTGRVSQLEIGEAIARPELRGLFRAAKQPLISATPARRPTAPPKSRATSTPTCSSARQAANVMTVPVAQRASLRLVKSLACSALGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRX4</accession>
    <name>A0A8I6XRX4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRX4"/>
    <dbReference type="SMR" id="A0A8I6XRX4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0210830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0210830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0210830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0210830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="44247" checksum="9EE75CB88AD39FC3" modified="2022-05-25" version="1">METAAGGLGLCDDVLVEILVRLPSKSVLRCRAVCKRWRRITTDGSFLSAHAAHAASCTREMIILPWSHYGPGSLAVKTMRIPLDPRPRSRPIRFLCNRTSKGWCRVLYSLDGLLVLEPRHGRSRFIICNPMTRQSASLPPLVPQPCFNAFACGFYLHSPSNEYRLLCHCVQGETDQARRHTEQSYYILSTGSTLPRRLGRAPTPVDLPTTWPRYEVPVAHRGILHWFSLHSEATRTGKMLAFHTVSETFRLMSRPPERAGGDTTTTSRSLFELDGELCVAAMQSVTSLDIWVLQDYKAETWMLRHRVTMLGVSNAVTMNMVLSVGSGTILIGSSNCDVARLYNLKDKKMHEDVMFESSEPTFLLFSESIVSHAFFDSPPHPQFESISFSSPRGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTR8</accession>
    <name>A0A8I6XTR8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTR8"/>
    <dbReference type="SMR" id="A0A8I6XTR8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0520750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0520750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0520750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF86">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0520750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="490" mass="55184" checksum="6AAB9717FAAD139A" modified="2022-05-25" version="1">MNNVLTAEYTKEEVKAALDHIGDLKSRGPDVLKVLNGDTFPEGWNSTTIVLIPRRIKDLRPISLCNVVYKLVSKVIANRLKIVLPQVISENQSAFVPGRLIIDNVLIAYEVSHYLMNKRNGTSGVAAVKADMSKAYDRVEWSFLEAMLGKLGFSNGWIALVMKCVTTVSYQIKVNGTFTQQFCPSCGLRQGDPLSPYLFVICAEGLSALLRHAEEQGVLHGVKICPRAPCVSHLLFADDSMLLIRAQQQEATTLLNILQLYEACSGQCINTEKSAIMFSPNTQDQDRIAVKDALAIRSEDWNDKYLGLPVHVRRSRKQAFRYLKRNMCGRVYVWQEKLLAKESKEVLVKAVGQAIPTYAMSCFDVTKSFYVELNALLGKFWWSQQDKNNSLHWLSWEKLTLPKAMGGLGVRDMHTFNLAMLSRQGWRIIQNPYSLCAGILKARYFPYCSALEAVAHDGISYSWRSILQGLDVVKQGYIWRVGDGTSINIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU14</accession>
    <name>A0A8I6XU14_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
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        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="103" mass="11864" checksum="F61C7F987EDD98DA" modified="2022-05-25" version="1">MIEDMNGRADDDFMRTWLTVVVSPFMCPTTSLHIRSLLGFMSRKKKAVNYCVHYMVVLYVDSLVIDIQVPDCPVRAEAWDNKRIVQVPKSKKIEGGYGKLRLK</sequence>
</entry>
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            <taxon>Hordeum</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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        <text evidence="1">Belongs to the serpin family.</text>
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0228200"/>
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        <property type="component" value="Chromosome 3H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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            <end position="225"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0228200.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25410" checksum="49207661567D9F3B" modified="2022-05-25" version="1">MSSTFSTAAPWTRRPSCGGRGGCTSIACHDGFRVLKLPYKAYSDDSPPAYDSKLRDGLPKFSMCVFLPEDRDGLWSLVDRITARPGFLREHLPTDHVPVGKFRLPKFKLTYMRSIRNVLQDLGLRLPFDRLRANMAGIVGDDDSAVPLCVSELVNKAVVEMNEEGCEAAAVTVESDEHMGFSLYSDYEYVPPPPTVDFVANHPFAFFIVEETSGTLVFAGHVLDPTKEE</sequence>
</entry>
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    <accession>A0A8I6XZE0</accession>
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        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
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        <lineage>
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    <reference evidence="3" key="1">
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0486740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="19180" checksum="E9BE004615612CA2" modified="2022-05-25" version="1">MCLRDQHLWRVRCPMICFYAVEWHLPHRVSKQFGVQQRTPPDYVETSVKLHMTNRQSNKTVINWEEHHITWVDMWNAQRQHRVENDETPDTDEAAYLRHLEWIRTEYRVIHKAAWTRSDCLDLLPSEAADAAINNSIRETVGAHLDYGPLHDRVVRPSFKI</sequence>
</entry>
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    <accession>A0A8I6XZE4</accession>
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        <recommendedName>
            <fullName evidence="10">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="11 12">
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A8I6XZE4"/>
    <dbReference type="SMR" id="A0A8I6XZE4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530490"/>
    </dbReference>
    <dbReference type="GeneID" id="123399410"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399410"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010087">
        <property type="term" value="P:phloem or xylem histogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000896">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000412">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053211">
        <property type="entry name" value="DNA_repair-toleration"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060">
        <property type="entry name" value="DNA DAMAGE-REPAIR/TOLERATION PROTEIN DRT100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060:SF21">
        <property type="entry name" value="L DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035280778" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="9">
        <location>
            <begin position="32"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="10">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="720" mass="74673" checksum="C0C3F5D9648371D3" modified="2022-05-25" version="1">MASATAQLSSEARFFFFLVFFSLSLVCCCCAGASNGTAATDDEAAMLLAIRASLVDPLGELRGWGSAPHCGWKGVRCDERGAVTGLDLAGMKLSGAIPDDVLGLAALTSVVLRGNAFTGGLPAALVSIPTLREFDVSDNGFAGRFPAGLGSCASLAHFNASGNNFVDLLPVDVGNATELETLDVRGGFFFGTIPESYGKLRKLKFLGLAGNNLSGALPAELFELTALEQIIIGYNEFTGPIPAAIGKLKNLRYLDMAISGLEGPIPRELGRLPALETVFLYGNNVGGEIPKELGNLSSLVMLDLSENALTGAIPPEVAQLGNLQLLNLMCNRLKGDIPSGVGELPRLEVLQLWNNSLTGPLPPSLGAAQPLQWLDASTNALSGPVPAGLCRSGNLTGLILFNNAFTGPIPASLTTCSSLVRLRAHNNRLNGAVPTALGRLHRLDRLELAGNRLSGEIPDDLALSTSLSFIDLSRNRLRSALPPRILSIPTLQTFAAAGNKLTGGVPDELGSCRALSTLDLSGNQLSGAIPAGLASCQRLASLILRSNRLTGEIPMAFATMPALSVLDLSNNLLCGEIPSNLGSSPALEMLSVAHNNLTGPVPATGLLRTINPDDLAGNPGLCGGVLPSCTASAPRASSSIHRRSHTKHNIAAGWVIGISLTLVACGAAFLGKVLYQQWYASGAVCCDAAKEAGTDSAAFQRLGFTGGKVIACVKEGNIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZV2</accession>
    <name>A0A8I6XZV2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZV2"/>
    <dbReference type="SMR" id="A0A8I6XZV2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0303220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="165" mass="18292" checksum="53D089B2F2A52A33" modified="2022-05-25" version="1">MCKISDDMRPHPVVVSALKMVCAVAPGWRVIPIPDIIDEVCKGPEMRKQVRSNPYIYRGNLPLKTCHELLMVSLDIEKNLHEVSLPFLVLHGGDDVVTDPSASKLLFEEASSADKAFKLYPGMWHALMAELPQDVERVYSDIISWLDKRANCAANVSMNTGTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0H4</accession>
    <name>A0A8I6Y0H4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123401489</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951247.1">
        <property type="nucleotide sequence ID" value="XM_045095312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0H4"/>
    <dbReference type="SMR" id="A0A8I6Y0H4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0568870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0568870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0568870"/>
    </dbReference>
    <dbReference type="GeneID" id="123401489"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0471490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0471490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0471490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0568870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0568870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0568870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401489"/>
    <dbReference type="OrthoDB" id="10253031at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0568870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="148" mass="15773" checksum="7E5E72BBE95DDBE4" modified="2022-05-25" version="1">MAGRKGGERKKAVTRSVKAGLQFPVGRIGRYLKKGRYAQRVGSGAPVYLAAVLEYLAAELLELAGNAAKDNKKTRIIPRHLLLAVRNDQELGRLLAGVTIAHGGVIPNINSVLLPKKSPAAAEKEATKSPKKKAATKSPKKKTAATKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2C7</accession>
    <name>A0A8I6Y2C7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123452444</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-8 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985027.1">
        <property type="nucleotide sequence ID" value="XM_045129092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2C7"/>
    <dbReference type="SMR" id="A0A8I6Y2C7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513780"/>
    </dbReference>
    <dbReference type="GeneID" id="123452444"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0426910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0426910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0426910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0513780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452444"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000056">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF29">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="14"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="583"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="665"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="712" mass="80046" checksum="C169917AEDF9805A" modified="2022-05-25" version="1">MPTIRAPASRQTATLQVAVKCRPLTDNESRRSRHIIQVIDDKNVAVLDPDVSKGYLDLIQNRTKEKRYSFDHVYAPGCSNTDVYKNISPTVLGVVQGLNVTVFAYGSTGSGKTYTMVGSRNDPGLMVLSFRTIFDQIKKDDSSDTFEVSCSYLEVYNEVIYDLLEKSSGHLELREDPEHGIIVAGLRRIKVHSADKILELLNIGNSRRKTESTEANATSSRSHAVLEITVRRKQKGKYGSQVLRGKLALVDLAGSERATETNNFGQKLRDGANINKSLLALANCINALGKQNKKGLAYVPYRNSKLTRILKDGLSGNSRTVMIATISPADDQYHHTTNTLKYADRAKEIKTHVHKNIGTIDNHVEDYQRMIENLQGEVSQLKKELADKEHQLSVKPTEKAADNELSWLNVLSQETGENVQERINLQKALFELEETNKRNRMELQHLDGAIARPQVNEKDYALFQALTSRRQVILDNIRDNDEAGAGYRKDIELNESRRRQLQDMIEEATSNNGNRTYLQILSQYRLLGMNNAELQIEMAMRDQVIHNQRESLRSLWNILYRTGLNQKQILKLAAKQGLTVEGCPLPSSSPESAMQHGRLPPFMSFPSPQSQSSSSPSSCFFQQGFSTMSVLKNQHETPTICRQEHLSSYYMMSGCSPYSGDGKQWASGGSTPYFSTPETKSEQSPCSAERRTPYSGIEDFSRQSKDSCSRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2M1</accession>
    <name>A0A8I6Y2M1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2M1"/>
    <dbReference type="SMR" id="A0A8I6Y2M1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0462570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0462570.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0462570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0556870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051606">
        <property type="term" value="P:detection of stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000470">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF90">
        <property type="entry name" value="OS11G0565920 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035167611" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="299"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="61190" checksum="E2FC738C1A275827" modified="2022-05-25" version="1">MPPPNSLLLLAVLLAASPHALAQQPPAAAACTPQERDALLAFKQGITISSDAAGLLASWRRKEDCCRWRGVRCSNRTGHVVALNLRGQELAGEISPSLLSLPHLEHLDLSSNSLVGPAGSIPEFLGSLRNLRCLNLSGMPFSGEVPPHLGNLSKLHYLDLSSYANVYSEDLSWLTRLPLLRFLSLIFVDLSMVSDWAHAVNALPLRSLHLEGCSLKSANQSLPHSNLTTTLEVLNLGVNKFDQPVASCWFWNLTRLKRLYLDYNNGGLYGPFPDALGGMVSLQELSFSAGRNTMSMASAHLKNLCNLRFLYLDNCFSNGFEAERLPQCSSSSNKLQELRLSGNQLTGPLADWMGHRTSLVILDLGFNKITGPIPEVIGRFTDLKALYLFGNHLTGHVPPEIRNLANLTWLDLSENHLNGLITEQHLHGLKSLQHVILSHNQLEIVVDSEWVPPFRLQEARFASCQMGPLFPAWLKWQVDLTHLDISSTRITDRFPDWFSSSFSKVTYLDISNNRISGALPKNMGNMSLGALYSSSNNISGRIPQLPRNLGTLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y320</accession>
    <name>A0A8I6Y320_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y320"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0299670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0299670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0299670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0299670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0299670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0299670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0299670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="19259" checksum="6F65759C23AE5BAB" modified="2022-05-25" version="1">MCLRDQHLWRVRCPMICFYAVEWHLPHRVSKQFGVQQRTPPDYVETSVKLHKTNRQSNKTVINWEEHHITWVDMWNAQRQHRVENDETPDTDEATYLRHLEWMRTEYRVIHKAAWTRSDCLDLLPSEAADAAFNNSIRETVGAHLDYGPLHDRVVRPSFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3Q8</accession>
    <name>A0A8I6Y3Q8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3Q8"/>
    <dbReference type="SMR" id="A0A8I6Y3Q8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0475770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0475770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0475770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0573880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF13">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="15957" checksum="AF5E748BB21204C8" modified="2022-05-25" version="1">MTRCTDCPRTAALKRLTSKEEKNGNFGREFVKCKSKPEGQIMKKCHHFEWMDDYIQRLQGLGLLDSRGDTIRDFNLPHDSAAPAAAARPEYPTVVDVELKTELKKMNKNFKQLIELKKQSNLIALGILALGIFYLMAISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5B2</accession>
    <name>A0A8I6Y5B2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ethylene-insensitive protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123450067</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983407.1">
        <property type="nucleotide sequence ID" value="XM_045127472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5B2"/>
    <dbReference type="SMR" id="A0A8I6Y5B2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0418520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0418520"/>
    </dbReference>
    <dbReference type="GeneID" id="123450067"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0348880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0348880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0348880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0418520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0418520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0418520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF107">
        <property type="entry name" value="ETHYLENE-INSENSITIVE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="52923" checksum="DF05638CBD70C982" modified="2022-05-25" version="1">MEAVQGVESLAGGDGRHHVSRTLGTALLISVGYIDLGKWVAAVDAGARFGYDLVLLVLFFNFSAVLNQYLCTCIGMVTGKNLAEISHQEYSQFICVGLGLQAGMSLFTSELTMILGIAVGYNLVFDQDDFITAIIFACLVINVLPYIFSPRDKRMAGTLNACIAGFTILCFVLGLLVSQPEIPLHVNVMFPKFSGESAYSLMALMGANIMSHNFYVHSSIVQVQKSHVHTVGGLFHDHLFSILFTFTGVFLVNYILLSSAAAESSHNVIHTFHDAVELMNEIFTSSMAPLVLLAVLLFSSHIIALTSVIASHVVTEHFFGVNISLFAHHVLLKLLSMIPAIYSAKVAGSEGVYQLIILCPVIQAFTLPSSIIPIFRIASSRWIMGNYTVSLRVEILAFLSFCLTLFINIIFAAEILFGDSAWTNSVKGNTETPVLIPHAVLILMSCASIAFALFLAATPLKSSGREAETQELCVHSQRGAPEEIQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y627</accession>
    <name>A0A8I6Y627_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LURP-one-related 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973249.1">
        <property type="nucleotide sequence ID" value="XM_045117314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y627"/>
    <dbReference type="SMR" id="A0A8I6Y627"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313190"/>
    </dbReference>
    <dbReference type="GeneID" id="123440766"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0261620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0261620.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0261620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0313190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0313190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0313190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440766"/>
    <dbReference type="OrthoDB" id="652749at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF56">
        <property type="entry name" value="PROTEIN LURP-ONE-RELATED 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0313190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21321" checksum="ED7FCE590E6F6338" modified="2022-05-25" version="1">MAKIQPLATAASRSPSDDQRSQQTYTVWMKSLVFNGNGCTVYGPDGAVAFRVDNYGCRGGREVFFMDRAGNALIRIRRKGFGAFRRWEVCRCAHNGGQEEEEATPWFTVRRAEKGGAAVAMHGGAGTCYKIDGCSARKSEYRIRGADGAVVAEVARKQTPAGVVLGEDVLTLTVAPEADHLLLLGLIVVRGLINRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y757</accession>
    <name>A0A8I6Y757_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SIAH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123403251</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953128.1">
        <property type="nucleotide sequence ID" value="XM_045097193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y757"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0621690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0621690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0621690"/>
    </dbReference>
    <dbReference type="GeneID" id="123403251"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0621690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0621690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0621690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403251"/>
    <dbReference type="OrthoDB" id="658368at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044286">
        <property type="entry name" value="SINL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632:SF10">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="7">
        <location>
            <begin position="152"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0621690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="347" mass="36917" checksum="94AC25CBDE07B11D" modified="2022-05-25" version="1">MGGKRKSTGDVKTPSTVGRRPGRPRKNTPPEAIRRPGRPRKSVPGVEVPVARGPASAQTLAPSRSEHEEDGNGGLLELARGAAAIAAAEEEEEQVKLRCDLCHGPLRPPIYQCTRVQHVACGDCGAGECRPCGGDPAAVFVHNAQLDALFGYIKVPCAFRKFGCASSVAYRDMASHEAACAWAPCACAECGYKGPPSGLLHHLTELSGRHAWTAHRITYGMDHQFAVPVPELSDQQCEDRGLLVAEDGALFLVAVGGVSDVRRVTVVCVRGNVGTGPVYSSSVEVAGPDEARRLKLENKVAASCSAPVEFDVLAQPECDTVPVYPEMLHGEELHLCIRIGKTKIICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7D2</accession>
    <name>A0A8I6Y7D2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123407066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956042.1">
        <property type="nucleotide sequence ID" value="XM_045100107.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956043.1">
        <property type="nucleotide sequence ID" value="XM_045100108.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956044.1">
        <property type="nucleotide sequence ID" value="XM_045100109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7D2"/>
    <dbReference type="SMR" id="A0A8I6Y7D2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635600"/>
    </dbReference>
    <dbReference type="GeneID" id="123407066"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0528090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0528090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0528090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0635600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407066"/>
    <dbReference type="OMA" id="ARNSCND"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF538">
        <property type="entry name" value="OS02G0227500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="28"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="330" mass="37227" checksum="A34B923959FD83BB" modified="2022-05-25" version="1">MAPLVVQHRPFICSSRRVVTPPIDYNEQAIIENLTESNQRSMWASRRIGEDEKVYLVHVQGTAGIGLPTTLVVKKFQNVNRCRSEMLLLATVRHENIINVLHVIERDEVSMLVYKYEVNGSLAYWLHRREGAGRPLSWPERRGIAIGVAKGLRHLHQGCNSQFVHHNINPSNILLDLDFNAKIASFGAAQVNMAGQGQPLRIPQFPIGNFGYAAPEYGIAPSQLTEKVDVYSFGVVLLELVTGRAANGAGPGGQLAMWARNNCNDLMANHLKRFKTEVDMEIPDQARYMKEMAAVFSLGVDCTVWDPQQRPSMRRALKRLRRSRRYSLWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9V1</accession>
    <name>A0A8I6Y9V1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Replication protein A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9V1"/>
    <dbReference type="SMR" id="A0A8I6Y9V1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0456210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0456210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0456210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF40">
        <property type="entry name" value="REPLICATION PROTEIN A C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A C-terminal" evidence="8">
        <location>
            <begin position="162"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0456210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="206" mass="22815" checksum="94BED1763F630D80" modified="2022-05-25" version="1">MDANGDATHALFTDEAMMVSPGRSTASTVIPDIEHYPILIEGEPVSSVILIGTVSQSNVRMDHWSFELCDATGMINVSYCNGDYVQVFGKTGMNEYFLQIKAFKIRPIVNYNDTTHHYLYSIYCTLDIRKTNALKAKLKPASSASAVFPKGPTITPIEDIAHGVSLKLIRSALDISQDEIMKVISEQIYLGLIYTTVDDNHFKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBL0</accession>
    <name>A0A8I6YBL0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBL0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0652340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0652340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0652340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0541410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0541410.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0541410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0652340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0652340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0652340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0652340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="92" mass="10269" checksum="E96BB639200B9835" modified="2022-05-25" version="1">MRLMQTYNADERPAGVGSQPTAAQQRPDTGAPQTPWRGVVATGGNQAVVSFAPYLISRKSRSGVDCFLFLFWAFTREWGHETSVDWGPFWFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBU5</accession>
    <name>A0A8I6YBU5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123451977</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="SMR" id="A0A8I6YBU5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0505330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0505330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0505330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0419730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0419730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0419730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0505330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0505330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0505330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000005">
        <property type="entry name" value="ARF guanine-nucleotide exchange factor GNOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000010">
        <property type="entry name" value="ARF guanine-nucleotide exchange factor GNOM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056604">
        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="11">
        <location>
            <begin position="521"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1383"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1403"/>
            <end position="1415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0505330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1415" mass="157473" checksum="8355C4F2650F2A1B" modified="2022-05-25" version="1">MGGLRAASPSPSPSPSSGVAMACVVASEVATVLAVMRRNVRWAGVRYGGDDGGGAEEEHLDHPLIAGLKSLRRRAAAWGSGWPEADPLLCLRPFLDVVRSEETGAPITGAALSSLHKLLSLDLVAPGGGAAEAMGAVVEAVTGCRFEVTDPASEEVVLARVLQVLLACVRGRAAPALANRHVCAIVSTCFRVVQQAGTKGELLQRVSRQTMQEVIRCVFVRLADIEPAAVVNEQIGKNQDLGAEELGNGKSDYVCLNSSGDEVGGGFGVVQDKAMMEPLGVPCIVEILQFLCSLLNIAEDMDVSQRMNGIDYDEDVPLFALGLINSAIELSASSIHRHPKLLAFVQDELFHNLMQFGLSMSPLILSTVCSTVFTLFYHLRQELKLQIEAFFSCVILRLAQGRYGASYQQQEVALEALVDFCRQKEFMAEMYANMDCDLQCSNVFEDLANLLSRSAFPVNSPLSALNVLALDGLVAVIQAIAERSDHAHQHHEQTVPEISEYFPFWQLKCESSNDPDQWVRFVHQQKSIKRKLMVGVEHFNRDKKKGFEYLQGVHLLPEKLDPRSVALFFRYTPGLDKNLLGEYLGNHDEFSILVLHEFAKTFDFEDMNLDAALRLFLETFRLPGESQKIQRILEAFSERYYEQSPHMFVNRDAALVLSYSVIMLNTDQHNVRVKKKMTEEDFIRNNRRINGGNDLPREFLSELFYSICRNEIKTIPEQGAGCSEMSYSRWVDLMWKSKRTSVYIACDSYPFFDNDMFPIMAGPSVAAISVVFDNVEHEEILTGCIDGFLSVAKLAAFYHLDDVLNDLVVALCKFTTLLNNSYADDPVIAFGVDTKARMATEAVFTIATSYGDHIRSGWRNIVDCILRLHKIGLLPGRLTGDTGDDQESSSDSLPSKLGSYAVAPQALPINTPKKTYGLMGRFSQLLYLDTEEPRSQPTEEQLAAQRNALETVKKCQIGTIFTESKFLQADSLSNLARALIQAAGRPQRITSSLDDEGTSVFCLELLITVTLNNRDRIVLLWQGVYEHITHIVQSTVMPCNLVEKAVFGLLHICQRLLPYKENLVDDLLRSLQLILKLDARVADAYCENITLEVTRLVKANATHIKSQMGWRTIISLLCVTARHPDASDAGFEALVFIMSEGAHLSPANFIVSVEASRQFAESRLGSAERSIHALNLMADSVNCLTRWSREVKEAGGEADRILEGIAEMWLRLVQALRKVCTDQREEVRNHALLCLHRCLVVDGISVSSSAWLMSFDIIFQLLDELLEIAQSYSPKDFRNMEVSLLHAVKLLCKVFLQSLKDLSAQSSFSKLWLEVLDMIEKFMKVKLRGRRTEKLHEAIPELLKNILLVMKANGVLSKTSASEENTLWETTWLQVNKIAPSMQPEVFPDNESDSAAKDEQSKSESPAQEGQTVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YD39</accession>
    <name>A0A8I6YD39_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6YD39"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0690730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0690730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0690730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0690730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0690730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0690730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
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        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
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        <property type="entry name" value="BNAC02G40300D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF11">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="218"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0690730.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="42362" checksum="FE7D9CCD2240B367" modified="2022-05-25" version="1">MPRPRSVAADFWTWTWDRSGTFSVKTAYRELKQQQSEMQGGRSSSAGDEETWKSLWKLNVQPKIRVFWWRVIKGFLPARAELVRRHVGDVATCALCGNADETLFHAFVTCNQATQFWAETLRFFGIKLPRLYQATWTRDLLDPKFIAKEEAAVIISVMWTIWGSRNKYTYGETMFQPLRSMEISQEHIRSLYIPAKQHSIPKMRKVWMNPDVGVIKINSDATIDAMGGRAATGVVARDHEGRFIVRRFCPYAVITDLYVSEALGCRDAMLLAIERRWPIIEISSDCKIVVEDWNGGRDRSSCGPIFREMTSYLSCFQGFELKFSGREANEVAHVLPKRGLSTEMQSVTMDVISDFLSAAVHSDYVRSLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDB8</accession>
    <name>A0A8I6YDB8_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDB8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669380"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0555420.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0555420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0669380.1">
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="148" mass="15668" checksum="D6B406CE6869D512" modified="2022-05-25" version="1">MDQPKKQQPTVAPSRAAPAAAAKKSTSTLLDAFEVDCIRRELESLLLKQNAGEGTASRSSEAAAEILGISRRHRHSSKKTSSAKNAIPAPPPPPPPPPPPAKKLSGGGTGLRLGKHAVKLCSGALAVFSPVENRRRPRRAGYREVEKV</sequence>
</entry>
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    <accession>A0A8I6YFL4</accession>
    <name>A0A8I6YFL4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NBS-LRR resistance-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8I6YFL4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0632830.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0632830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0525540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0525540.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0525540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0632830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0632830.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0632830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF64">
        <property type="entry name" value="OS12G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="247"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0632830.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="35054" checksum="3810A35A3A9CD1C1" modified="2022-05-25" version="1">METAIGAAGWLVGKVLDKLSNDLVAAYVASSELRLNSEQIKTKLKYMQGLLHAAQDRDVSTNPGLQSLLEDLSKKADEAEDALDELHYFMIQDQLDGTEEAASELGDGLHGHALHVRHAARSIVGNWLTCFSCSRTQDDDYVDATVVATNNREDTTRSNSGNDGGHVGKLKFDRVAMSTKIKSVIESIHSLCDPVSDLLNKIPNINIAIAMKRAPTGSVVAQDKLYGRRVIFEQTVSVLTGSTYLGETLSVLPFVGPGGMGKTTFTQHLYNDKRVEEHFTIRVWVCVSTDFGVLNLTRLILKCIPAIENENTVVQLKQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFW1</accession>
    <name>A0A8I6YFW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">hydroperoxide dehydratase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123402023</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the biosynthesis of jasmonic acid, a growth regulator that is implicated also as a signaling molecule in plant defense. Converts 13-hydroperoxylinolenic acid to 12,13-epoxylinolenic acid.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Plant hormone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.92" evidence="15"/>
    <dbReference type="RefSeq" id="XP_044951813.1">
        <property type="nucleotide sequence ID" value="XM_045095878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFW1"/>
    <dbReference type="SMR" id="A0A8I6YFW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542940"/>
    </dbReference>
    <dbReference type="GeneID" id="123402023"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451570.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0542940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0542940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0542940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402023"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009978">
        <property type="term" value="F:allene oxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11071">
        <property type="entry name" value="CYP74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000024">
        <property type="entry name" value="Allene oxide synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF49">
        <property type="entry name" value="INACTIVE LINOLENATE HYDROPEROXIDE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 16" id="KW-0349">Heme</keyword>
    <keyword evidence="8 16" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="547" mass="60249" checksum="2F292052B8688C67" modified="2022-05-25" version="1">MTISLYTVLTSPSRRRQPLALPPREPHLLSSLYSTEKLRYPARASHHRIFGASVRPCVPAMLPSFSPAVTAAAMAPPPPKPIPGGYGAPVLGPLRDRLDYFWFQGPEEFFRRRAAQHRSTVFRANIPPTFPFFVGINPRVIAIVDTAAFTALFDPELVDKRDCLIGPYNPSDSFTGGTRVGVYLDTEEPEHERTKAFAMDLLRRSSRVWAPEFLEGVDGMLADIESDLAAGKEGGASFLVPLQRCIFRFLCRSVASADPAAEGLVDRYGLFILDVWLGLQLLPTQKVGAIPQPLEELLLHSFPFPSILAKPGYDLLYRFVAKHGAESVAVGVNDHGMSEKDAINNILFLLGFNAFGGFSVFLPFLILQIGKDAALRARLRDEVRAALDQHDGEVGFASVKGMPLVRSTVYEVLRMNPPVPLQFGRARRDFVLRSHGGEGFSVAGGEMLCGYQPLAMRDPEVFERPEEFVADRFVGAGGEALLRYVYWSNGPETGEPALGNKQCAAKDVVIATACMLVAELFRRYDDFECTGTAFTSLKKRPQPQPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGF1</accession>
    <name>A0A8I6YGF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SCO1 homolog 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123396372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947262.1">
        <property type="nucleotide sequence ID" value="XM_045091327.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044947263.1">
        <property type="nucleotide sequence ID" value="XM_045091328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGF1"/>
    <dbReference type="SMR" id="A0A8I6YGF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0476620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0476620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0476620"/>
    </dbReference>
    <dbReference type="GeneID" id="123396372"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0394800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0394800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0394800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0476620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0476620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0476620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396372"/>
    <dbReference type="OrthoDB" id="270009at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF1">
        <property type="entry name" value="PROTEIN SCO1 HOMOLOG 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237141" description="Protein SCO1 homolog 2, mitochondrial" evidence="2">
        <location>
            <begin position="21"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0476620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31559" checksum="401599E67F1BDE2D" modified="2022-05-25" version="1">MLPARLLRLSLVRRLGAARAAGVPPPWQTRIFPVRGYQARGYSTGGSSKYDKPMRQFAEENESNSQPLIYYIVPSAILAFAGLGTYVHYNDEKRAVPLGKQAEQSGITKRCTTNRPAIGGPFKLYDTENNVVTESKLRGNWTLMYFGYTSSPDVGPKEVQKIADVVKLLESKYDIKIKPLFVTLDPQRDSPAHLKAYLSEFDPRIVGLTGSVDAIRQMAQEYRIFFKKVGEVGQDYIVESSHNMYLLDPCLETVRCFGAEYKASDLAEAVTTEIQKASNSAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIF9</accession>
    <name>A0A8I6YIF9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123408732</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="2">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIF9"/>
    <dbReference type="SMR" id="A0A8I6YIF9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0710110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0710110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0710110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0710110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0710110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0710110"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF2">
        <property type="entry name" value="BLUE-LIGHT PHOTORECEPTOR PHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="4">
        <location>
            <begin position="163"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="416"/>
            <end position="420"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="468"/>
            <end position="475"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0710110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="504" mass="54016" checksum="0095E326BD22B135" modified="2022-05-25" version="1">MVGGIEKKSSTTFPPVRLPPTHLASPPSSPRASSEPNPLPHLNYRHPAPPPPRHPSMASASDSDPAAHPRDDPNLPFASFSLSLSLRTPTAHATLASVPSAVHLPTQISTLAVCLHPSAASSSPRRATRLNAAAASLLSPLTASSPGLSRSFPSGAPGAAGRRRTLVWFRADLRLHDHEPLHAAVGASSSLLPVFVFDPRDFGKSPSGFDRTGPYRASFLLDSVADLRRSLRARGGDLVVRVGRPEVVIPELARAAGAEAVYAHGEVSRDECRTEEKVSKAIEKEGVEVKYFWGSTLYHLDDLPFRLNDMPSSYGGFREAVKGLEVRKVLDAPEEVKCVPMKNVLEPGDIPTLGELGLSAPPAMAQDSKSAAGSNLIGGEAEALERLKKFAAECCMQPNKAVKDSTQNSIYGANFSCKISPWLATGCLSPRFMYEELKKHAIRAIPSGSTPKDGDGTSDAGTNWLMFELLWRDFFRFVTKKYSSAQKTSEVAPATGCTPTPAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJU7</accession>
    <name>A0A8I6YJU7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HAUS augmin-like complex subunit 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123407247</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956273.1">
        <property type="nucleotide sequence ID" value="XM_045100338.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956274.1">
        <property type="nucleotide sequence ID" value="XM_045100339.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956275.1">
        <property type="nucleotide sequence ID" value="XM_045100340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJU7"/>
    <dbReference type="SMR" id="A0A8I6YJU7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659660"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659660.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659660.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659660"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659660.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659660.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659660"/>
    </dbReference>
    <dbReference type="GeneID" id="123407247"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0547410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0547410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0547410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0659660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0659660.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659660.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0659660.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659660.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407247"/>
    <dbReference type="OrthoDB" id="2159690at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031023">
        <property type="term" value="P:microtubule organizing center organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026206">
        <property type="entry name" value="HAUS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032733">
        <property type="entry name" value="HAUS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19378">
        <property type="entry name" value="GOLGIN- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19378:SF0">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14932">
        <property type="entry name" value="HAUS-augmin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02089">
        <property type="entry name" value="HAUSAUGMINL3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAUS augmin-like complex subunit 3 N-terminal" evidence="11">
        <location>
            <begin position="28"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="545"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659660.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="617" mass="69592" checksum="8E9787EDC093F2E3" modified="2022-05-25" version="1">MSGAALCAALTELGFDGEDPLDADALEWPFQYEEARPLLAWICSCLRPSNVLSPSHLSQYEQLVEEGRLLEGEDLDSAFDSISAFSSKKDNQEAVFEAEETILDIREAKLAYRAEVFELQKQLARQQAQFDMLAGQASSLIQGRRARVSAMSAVSAQLISLDEILSSRNLEMNAVLGRITATTQELAHYHSGDEDSIYLAYSDFHPYVIGDLACTKELNRWFSKQFEKGPFRLVAEEGKSKCSWVSLDDITNGLARGDSEKSHHHQRVAELQRLRSIFATSERQWIEAQVENAKQQAILQILKSQVSSDEAHIHRDIHSLRRKSSELAGELSTLTQKVQPFISETIPCLCSELAQLQGTYILQGDYDLKVMRQEYYINRQKTFINHLVNQLARHQFLKIACQLERKHIASAHALLRVIESELHSYLSAVNTRLGHCSSLIQAAAEVREQGAIDDRDTFLHAVRDLLCIHSNSQAAVPTYMSAHALVQQISALQSDLLSLQSELENTLPADRKRCINELCTLIQTVEQLLFASSTTAEPVLTPWPLMRALDDMENANAQVEVAVEEVTKARTQKIKIFENRAHEVGRERQVFVDFFSNHERLKNQVRELTSCVKALQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YLT9</accession>
    <name>A0A8I6YLT9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Retinaldehyde dehydrogenase 3</fullName>
            <ecNumber evidence="8">1.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Aldehyde dehydrogenase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Aldehyde dehydrogenase family 1 member A3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123403756</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. High specificity for all-trans-retinal as substrate, can also accept acetaldehyde as substrate in vitro but with lower affinity. Required for the biosynthesis of normal levels of retinoate in the embryonic ocular and nasal regions; a critical lipid in the embryonic development of the eye and the nasal region.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>all-trans-13,14-dihydroretinal + NAD(+) + H2O = all-trans-13,14-dihydroretinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:194182"/>
            <dbReference type="ChEBI" id="CHEBI:194183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:75120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>all-trans-retinal + NAD(+) + H2O = all-trans-retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:35291"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:42081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>retinal + NAD(+) + H2O = retinoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16177"/>
            <dbReference type="ChEBI" id="CHEBI:15035"/>
            <dbReference type="ChEBI" id="CHEBI:15036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16178"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; retinol metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.36" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YLT9"/>
    <dbReference type="SMR" id="A0A8I6YLT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0611990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0611990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0611990"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001758">
        <property type="term" value="F:retinal dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002138">
        <property type="term" value="P:retinoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000054">
        <property type="entry name" value="Aldehyde dehydrogenase family 1 member A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="18">
        <location>
            <begin position="1"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0611990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="423" mass="45462" checksum="9A2390E8314BA44D" modified="2022-05-25" version="1">MTAYERCRVLLRFADLIEQHNDEIAALESWDGGKPLEQSAGAEVPMAARCMRYYAGWADKIHGMVVPADGPHHVQVLREPIGVAGQIIPWNFPLLMFAWKVGPALACGNAVVLKTAEQTPLSALYVASLLHEAGLPEGVLNVVPGFGPTAGAALCSHMGVDKLAFTGSTGTGKIILELAARSNLKPVTLELGGKSPFIVMDDADVDQAVELAHRAIFFNQGQCCCAGSRTFVHESVYDEFVEKSKARAQRRVVGDPFKKGVEQGPQIDGQQFKKILGYVKSGVDSGATLVTGGDRLGSRGFYVQPTVFADVEDEMKIAKEEIFGPIQSILKFRDVGEVVRRANATQYGLAAGVFTGSLDTANAVARALRVGTVWVNCYDVFDAAIPFGGYKMSGVGREKGPYSLGNYLQTKAVVAPLRDPAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMB8</accession>
    <name>A0A8I6YMB8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123398840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949222.1">
        <property type="nucleotide sequence ID" value="XM_045093287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMB8"/>
    <dbReference type="SMR" id="A0A8I6YMB8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518900"/>
    </dbReference>
    <dbReference type="GeneID" id="123398840"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0518900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398840"/>
    <dbReference type="OrthoDB" id="666391at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF309">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="7"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="692" mass="76860" checksum="668BAF8CC79020BC" modified="2022-05-25" version="1">MPPAPELSMDLVEQFLIRLPPDDPGCLFRASLVCRLWRGLLTSADFARRYREFHRTPPLLGFFENHDTVKPWFAPSSPAASPFPPLFPDRRDLYVLDSRHALVLLRNPIRTDDNDHAVSLIVWDPVGRRQWEFPPPDFASTVIYDVGVVVCAADHCDHLGCHGSSFLVGYVGTDYSSAYASVFSLETSSWSPTVSCPHPGIEIWRCQPKPLVGNAAYSICVSEDEEYGVSQLVLRFDLFSRELSKFDGPTIHRHSHYVLMKTEEGVLGCAAMQGSRSRLHLWSTETGPDGAVAWMQPRVVHLNSSFFGVVCFEDRAGAFYLTTDDGVTTVDLKSGRVKKTSIDNSSTIPYISFYTPDQAVQNVEVAQGGRWKQVGDKEKEADEKEEQEEEKALKELFTKGSKALKDEDFISAVDCRRTILEIRVARHGKISPKCCSAYFNYGWALLRKILSNRDSVNSTTTQRNTGRKISEDLDLAWKMLHVARAISAKIPASTIEKYKIYAVLAKVSVEREDTDYSLVACFKALANLEHLLEPDHRRVINLNLHIRFAFELQSKIGDSKAILLCMSRVENLKRARQALLADKGNDISATEVGSENTFPPKLIEFFTNKILSALEEKLEDPGASNTRVSEASAGADHEAAGRIVKRAKLKQTSVEDDSSDSSDGDTEGYDDSNGSDGDSDGSDGDSDGSAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMW4</accession>
    <name>A0A8I6YMW4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LURP-one-related 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123401193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950861.1">
        <property type="nucleotide sequence ID" value="XM_045094926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMW4"/>
    <dbReference type="SMR" id="A0A8I6YMW4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620260"/>
    </dbReference>
    <dbReference type="GeneID" id="123401193"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0514530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0514530.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0514530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0620260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401193"/>
    <dbReference type="OMA" id="CLMDVAG"/>
    <dbReference type="OrthoDB" id="652749at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF25">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 2B FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="28092" checksum="8F0FC8293B457E5E" modified="2022-05-25" version="1">MGGARWCLGAELWTPKVKVHSSASCSSQHRLLRQDTPSAGAPPAEAAAVQVEVVKEQDEGEEHRSQQREVFTIWMKSLVLNGSGCTVFDSRGRIVYRVDNYGSHRSVDVCLMDITGSVVLQVLKKFRRWEGYRCGGWEAEPPERARAADTPRRRPWFTVVSNKWGSGPRCEFRSDGHAVRYKMDGGRRRQQAARASWIVDDATGLAVAEVKRKLTATGVSLGEDVLTLVVEPNVDHSLIMGLLVVHGLINHSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNF1</accession>
    <name>A0A8I6YNF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123407112</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044956098.1">
        <property type="nucleotide sequence ID" value="XM_045100163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNF1"/>
    <dbReference type="SMR" id="A0A8I6YNF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0702610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0702610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0702610"/>
    </dbReference>
    <dbReference type="GeneID" id="123407112"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0582860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0582860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0582860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0702610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0702610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0702610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="835"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="1015"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="518"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="518"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0702610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1174" mass="129245" checksum="6E72802CEDA87D12" modified="2022-05-25" version="1">MGCISKKNMAAPEQGPITLPGKKKCFACKETSHNLDQCRIKDKLGTVARLFGRSTSFPFYMIQPSKEVVKNEKLYHHCLLITSNTCNLDPARVKTELNKFWELTDDWDIRREGRQNFVASFNSEDDLLSCLKPPNVETFLDEKEVNFSVAMWDGGDGEKLDLIKEWLLVYGVPDAYRNWKELYQVASAVGVLLEVDEESLESGDKEPIRLRIALGSLVGAPFSYHFVFGWSSRLVEFMIEDKVRRIEGQWKEVEERNVSVMKEYADAREEGIEVPPETMNKGIDLEGTGVDFAGNSSLDFRSCSGKECKEELKTTEQNGSVRKEYADIREEGIEVPPETLNEGMDLEATGVDFTGNCSFDLGSCSGKECKEELKNTETATLLEAAKFIAELRADEDIKGNSASAMAATTTNSKNAAKGGQSPSENSAGGYHISGLEDCSDKERQKKLKTCDMATFLQASKFTEELRIDGKIKENNASAILGTRTNSERATEDIPKATEAAKFIAELRDDEDIKGNSASAMAATTTNSKNAAKGGQSPSENSAGGYHLLGLEDCSDKERQKKLKTCDMATFLQASKCTEELRIDGEIKENNASAIVGTRTNSERATEDIPKATEDMATLLQVSKFTEELRTDGEIKENSASAIVGTRTNSERATEDIPKAEGMDKPAIKNVYTRRNRKQKESTEGSTLFAHINSDSTCEGIQKPPIKNAYEGSDPKQKGYTGGQVASMELNKAMNPGSSSHADCTRNKTEAKTLGAEGAQSIKGSSACMIRVTRSKGVDEAGTTLQGDRLTQFYEEFNTYNEIYDSFVEMGLRENLLKGIYAYGLEKPSAVHQRGIVPLCKGLDVIQQSLSGTTVTLACGVLQRLNYGSTECQALILVPTRDLAQETEKVIGALGQWLGVKAHACLGGTSIRGDKQSLSNSTQVIVATPSRALDMLRGQALCPDNIRMFVLDQADETLAGGLKNQIYDIIQLLPAKIQFAVFSTVMSHEALELCRKCMNKPMKVIVPKDEELESFSIKQFYVKAEEEELKFEKLCDLFDTMAVKQNIIFVDARQKVKSLTEKIRGRGYTVSASHGGMNQHERDVAVQDLRSGSSLVLVTTDLRGADTLQVPVVINYDLPTQAIQYIRHVRRSGQPGGNGVAISFIAPADRRVLSDIQRFCNAQMEELPSNVADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPB9</accession>
    <name>A0A8I6YPB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123395221</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946109.1">
        <property type="nucleotide sequence ID" value="XM_045090174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPB9"/>
    <dbReference type="SMR" id="A0A8I6YPB9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0536930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0536930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0536930"/>
    </dbReference>
    <dbReference type="GeneID" id="123395221"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0536930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0536930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0536930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009553">
        <property type="term" value="P:embryo sac development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009860">
        <property type="term" value="P:pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000022">
        <property type="entry name" value="protein EXPORTIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0536930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1062" mass="121816" checksum="E45AF92B7587F57B" modified="2022-05-25" version="1">MAEKLRDLSQPIDVPVLDATVAAFYGTGSKEERSAADLILRDLQNNPDMWLQVVHILQHSQNLNTKFFALQVLESVIKYRWNALPVEQRDGIKNYISDVIVQLSSNEVSFRQERLYVNKLNIILVQVLKHEWPARWTSFIPDLVAAAKSSETICENCMAILKLLSEEIFDFSRGEMTQQKIKELKSSLNSEFRLIHELCLYVLSATQSSELIRATLATLHAFLSWIPVGFIFESPLLETLLKFFPMAAYRNLTLQCLTEVAALHFGDFYNMQYVKMYTFFMLQLQAILPPATIPNAYANGSNEEQAFIQNLALFFTAFFKNHIRILEASAENRAALLVGLEYLIGISYVDDTEVFKVCLDYWNVFVLELFEAHNQMEPAIPAAQMIPGVDGTGTAVHQRRQLYASPLSKLRMLMICRMAKPEEVLIVEDENGNIVRETMKDNDVLVQYKIMRETLIYLSHLDHEDTEQQMLKKLTKQLNGEDWSWNNLNTLCWAIGSISGSMVEEQENRFLVMVIRDLLNLCEITKGKDNKAVIASNIMYVVGQYPRFLRAHWKFLKTVVNKLFEFMHEMHPGVQDMACDTFLKIVQKCKRKFVTQQVGENEPFVSELLTNLATTILDLEPHQIHTFYESVGHMIQAESDNTKRDEYLKRLMSLPNQKWAEIIGQAGQSIDILKNQDVIRSVLNILQTNTSVATSLGPHFFPQISLIFLDMLTVYRMYSELVSSTIAEGGPYASKSSFVKLLRSIKRETLKLIETFVDKAEDLPHLGKQFVPPMMDPILGDYARNVPDARESEVLSLFATIINKYKAEMLDDVPRIFEAVFQCTLEMITKNFEDYPEHRLKFFSLLRAIGTHCFKALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEILKNFQASEFTNQFYKTYFLNIEQEIFAVLTDSFHKPGFKLHVLVLQHLFCVVDSLTEPLWDASTVSYPSNALFVRDYTIKLLGASFPNMTVAEVTKFVDGLLGSRHDLPSFKNHIRDFLVQSKEFSAQDNKDLYAEEAAVQREKERQRMLAIPGLIAPSELQDEMVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YR24</accession>
    <name>A0A8I6YR24_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YR24"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0565130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0565130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0468420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0468420.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0468420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0565130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0565130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0565130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0565130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="16173" checksum="612E5A4485539048" modified="2022-05-25" version="1">MNFPDIHTLQQALDLAPPPRLNSAQHRAEHTAVQRRLLVAQEDERVMAEWRRRHPEDVAYEQEYWTGRREEDTRRRREERLDRRRRKALACAQADLVNAGGRSFFTEEYERWFDIWLSTSDDTNDDDDGADDWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXF0</accession>
    <name>A0A8I6YXF0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cysteine-rich transmembrane CYSTM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123410449</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYSTM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959329.1">
        <property type="nucleotide sequence ID" value="XM_045103394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXF0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715950"/>
    </dbReference>
    <dbReference type="GeneID" id="123410449"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0715950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410449"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044850">
        <property type="entry name" value="WIH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31568:SF127">
        <property type="entry name" value="CYSTEINE-RICH TRANSMEMBRANE CYSTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31568">
        <property type="entry name" value="RCG49325, ISOFORM CRA_A"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="12975" checksum="47784FD33F1CF235" modified="2022-05-25" version="1">MQALGKALFLILPIPFPPQQQSLFPSSSSSLVLDRFRKTLISNSIMSYQQGATATAYPPQQQQAYVAAPPPAGYPQKDQQYPAAGGADTTTSRGGHGHHHGGGFWRGCCAALCCCCLLDACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z041</accession>
    <name>A0A8I6Z041_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z041"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0564010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0564010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0564010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0564010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0564010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0564010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF87">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="2">
        <location>
            <begin position="30"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0564010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="518" mass="59849" checksum="EDB4A74544B509C7" modified="2022-05-25" version="1">MEALIGMSPQELEVEDGGKKDRVPAGATFTWIAANFAHCPEDADDEVIQRYARVYMWYVISRTIFADGTGKNAPWMWLKALTVLDNKFSWGSAALAYLYRQLDDACRRTTKDDGVGGCMLLLSVWSWERLPVGRPKSSKWNTWDDHDNPVRQPTWAYKWDLVSEVASEVNLLYKQYTNEMDSLTPEQVEWEPYGVGTNFGDAHTFELNALCVQERHLWLMRCPLICNWAVEWHLPHRVLRQFGYFQPHPPEWVDTDTQLHRLDRRRQRKIKDWQKHHKSYVLMFEQSESTRVEICPPAYEEDILEEPTEYDELAQGGYSKLIHEGYQTSFAPVLNFVRKEVKKQADESEDILDNTPGGKKGESALRLFIKEQGKKLRRLSNNLGCRDPEYVSPSRSGSSERNTLDDSASSGARMDDGDITQRNTQEDDVDTPQAVDDMTLKAFQRSAYMLKPRKEFKRYSSDDYAEGKNPVAGSSRLSRMRSREDEVEDDDDDESDDPDQFVVARRKKLVSKRGRGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z3C7</accession>
    <name>A0A8I6Z3C7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401915</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044951714.1">
        <property type="nucleotide sequence ID" value="XM_045095779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z3C7"/>
    <dbReference type="SMR" id="A0A8I6Z3C7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612790"/>
    </dbReference>
    <dbReference type="GeneID" id="123401915"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0508120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0508120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0508120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0612790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401915"/>
    <dbReference type="OMA" id="AFSDHNC"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035340703" description="Endoglucanase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="11">
        <location>
            <begin position="25"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="497" mass="54370" checksum="5D7A1FDA55354283" modified="2022-05-25" version="1">MGLSSALVLALLLLPAVASAGHHDYGDALHKSILFFEGQRSGRLPPDQRLRWRRDSGLHDGAAAGVDLTGGYYDAGDNVKFGFPMAFTATLMSWGLIDFGRSFGPHKEEARKAVRWATDYFMKATAKPNTVYVQVGDAFKDHSCWERPEDMDTPRTVYKVDPSHPGSDVAAETAAALAAGSIVFRDADPVYSQRLLDRAMAVFKFADRYRGAYSSSLHAAVCPCYCDFDGYQDELLWAAAWLHKASRRREYRQYIKKNEVVLGASDSINEFGWDNKHAGINVLISKEVLMGKDEYFQSFRVNANNFMCTLLPGISDHPQIQYSPGGLLFKVGGSNMQHVTSLSFLILAYSNYLSHAGAHVSCGGGGRTAPPTKLRQVAKRQVDYILGDNPLRMSYMVGYGPRFPRRIHHRGSSIPSVAAHPARIGCKAGAAYYATAAPNPNLLVGAVVGGPTDATDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAEWADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z3M2</accession>
    <name>A0A8I6Z3M2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z3M2"/>
    <dbReference type="SMR" id="A0A8I6Z3M2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0586410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0586410.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0586410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0707100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707100"/>
    </dbReference>
    <dbReference type="OMA" id="CHCAYKY"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002608">
        <property type="entry name" value="Os03g0582100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000288">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g02750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000458">
        <property type="entry name" value="Pentatricopeptide, putative, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF530">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="376"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="556"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="648" mass="72138" checksum="57726621D2A50908" modified="2022-05-25" version="1">MPPILLPPTPCAVNSTSRTAGDAITHRNRKLTTRRQHCRISVTTSCSSSPLSVAPALIANDPTARAFSALFKAASSPSPASPSLGAQLHAQAVVRGFLRDGDNTILSTAILNFYASCREPDLARRVFDRMPRRSAITWNALIKGYAHAGRRDEALALFRCMTRQDRVAPDKYTFPALLSGIGRNHGGGCVRELGGAVHAQVVKSGLDKDPFVGASLVSMYAATGALEDVKAVFDDVDTLDPVVWSSVISAYVNCKDEEGALITFYKMLCQDIKPRQFVYSSLFAICGSMSMLEMGRQVHAHSLKNITNKDAAMTNALLTMYSDCGCISDAWRVFNSNDIVDVISYNSMILALGQHAHPKEAAELFRRMKFSGLIPDEVTFLNLLSAFNHAGLVHEGLHLFDSMMDMEGIEPMYQHYACVIDMLARSGEIEESMKTIKEMPFEAETPLWRIVLGACSKHGDIETGMKIAKMLFHMEPYEPTNYILLGNIYARLGRWTDTEKIRSLMEKRGIDGDDAFSWIEMGQRTHRFGVDDRSHPISEEIYRNLDRLIGSIKAAGYVPDTSFATHSTEKDRREESLRYHCEKLAFAFGDLASPSGDTLRIMKNLRVCGDCHSAYKYFSLVTGRKIVLRDNHRFHHFYKGACSCGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z7T8</accession>
    <name>A0A8I6Z7T8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC123412741</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="RefSeq" id="XP_044961611.1">
        <property type="nucleotide sequence ID" value="XM_045105676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z7T8"/>
    <dbReference type="SMR" id="A0A8I6Z7T8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734250"/>
    </dbReference>
    <dbReference type="GeneID" id="123412741"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0609160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0609160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0609160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0734250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412741"/>
    <dbReference type="OrthoDB" id="643280at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000537">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035265260" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="22"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="457"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="23"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="356"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="513"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="810" mass="87770" checksum="E5A2A6C2665DF2E8" modified="2022-05-25" version="1">MARVAATFMALACLCLAPAVGAPATVSARRPLRGNDTLVSAQGNFELGLFSPAGSSGDRFYLGIWYKSIPGQTVAWVGNRATPLSGLASAELRVSADDGNLELVGPTSASSLPVVVWSSNLSSSSLSSTPGSNNTAEIRDDGNLVLVDGANSSNVLWQSFDHPTDTYLPGAWLGENKRTGEYQALSSWRNAQDPAPGMFYDTVDPNGTAEFFMLWNRSRVYWRGGVWTGGRWSGDVGAATGRGTNLYNTTYVDTPAFRGTTTVLSDNATITRLVLDLNGQKKQFVWVPASRSWQLFWAAPTAQCDAYALCGNFGVCNQRSQLPCRCPPGFAPVSERDWTLSDWSGGCRRSSPLTTCAHNGSTTDGFLALPDVKLPDEPLAVGTARSKAECESTCQNNCSCQAYAFSAGCTVWHGDIRNLEQLFADSSSPGSDFYLRLSQRALQDLHNVHKKKEGTKLWLVVGITLTGVAALGASVILAWRILLARRRLAVSMENENGSSLAVYSYGDLRAATNNFSERLGGGGFGSVYRGVLKQQKGGNTTQLQVAVKKLEGFARQGDKQFRAEVSTLGLIQHVNLVRLLGFCSSGDEKMLVYEYMPRGSLNGSFFDGGACPSWRDRYRVMIGVARGLAYLHDGCRERIIHCDIKPENILLDEDMSPRIADFGMAKLVGRDFSRALTTMRGTIGYLAPEWISGQPISAKADVYSFGMVLFELISGRRNSERFGELEAAGTFFPVWAAEKVVEGEVGAVADPRLHGDVMPEELERACRVACWCIQDEEAHRPTMAQVVQALEGAVVVHAAPVPRALQRLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z8Y0</accession>
    <name>A0A8I6Z8Y0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z8Y0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0669960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF87">
        <property type="entry name" value="OS01G0158850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="23019" checksum="25631A0D5AD2893C" modified="2022-05-25" version="1">MVMVAGSDSDIANLKFLFLCFEEMFGLKINFDKSEVVVLGYSDEEQHRIADKLNCKLAVLPISYLGMPLAESRILVSGFDLLVGRGASRAEPWYIRFTSKGIKTVLISANLASLPMYMMGMYILPEGVHSSFDKELARFFWQAGDGRPKYHMVKWADICLPKDRGGLGIPASRCMNVALMLRWVWRILQGDGGLWLQLIEAKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1T1</accession>
    <name>A0A8I7B1T1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123452666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985302.1">
        <property type="nucleotide sequence ID" value="XM_045129367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1T1"/>
    <dbReference type="SMR" id="A0A8I7B1T1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0094820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0094820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0094820"/>
    </dbReference>
    <dbReference type="GeneID" id="123452666"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0078200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0078200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0078200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0094820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0094820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0094820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452666"/>
    <dbReference type="OrthoDB" id="6605928at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0094820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="42863" checksum="B6DAFD1371CE566E" modified="2022-05-25" version="1">MATRYWIAALPVADDNVAAGKTALWARLQEAISRHSFDTPLYRFTVPDLRPGTLDSLLALSDDLVKSNIFIEGVSHKIRRQIEDLERAGGVEPGTLTVDGVPVDSYLTRFVWDEGKYPVNAPLKETVASIQSQVAKIEDDMKVRVAEYGNVKSQLGAINRKQTGSLAVRDLSNLIKPEDMVTSEHLVTLLSIVPKYSQKDWLASYESLDTFVVPRSSKKLYEDNEYALYTVTLFAKVVDNFKVHAREKGFQIRDFEYSPEAQESRKQELEKLLQDQEVMRTSLLQWCYASYSEVFSSWMHFSAVRVFVESILRYGLPARFLSVVLAPSTKSEKKVRNILEGLCGNTNSSYWRSEDDVGMAAGLGGETESHPYVSFTINFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1W8</accession>
    <name>A0A8I7B1W8_HORVV</name>
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        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1W8"/>
    <dbReference type="SMR" id="A0A8I7B1W8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0099090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF233">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 72-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="240" mass="26873" checksum="FF0E596ED4EEF32C" modified="2022-05-25" version="1">MYRHDPWELPERALSGAREWYFFTPRDRKYPKGSHPSRAVGGGYWKATGADRPVARTGRTVGVKKSLVFYHGRPGAAVKTDWIMHEYRLAGAEGRAGRKRDGASLRLDDWVLCRLYNKKNQWEKMQRQRQEAAVKAAASRSASGGETRTPEFNVDNDAFPELDSLSTALQGMGTTNAAMLPKEEVQELELATDEWLMGISLDDLQGPGSLMLPWDDSYAASFLSPVAAMKMEQNIGPFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3N1</accession>
    <name>A0A8I7B3N1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123444237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976838.1">
        <property type="nucleotide sequence ID" value="XM_045120903.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976846.1">
        <property type="nucleotide sequence ID" value="XM_045120911.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976853.1">
        <property type="nucleotide sequence ID" value="XM_045120918.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976860.1">
        <property type="nucleotide sequence ID" value="XM_045120925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3N1"/>
    <dbReference type="SMR" id="A0A8I7B3N1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070000"/>
    </dbReference>
    <dbReference type="GeneID" id="123444237"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0070000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0070000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0070000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444237"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="3">
        <location>
            <begin position="52"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0070000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="126" mass="14526" checksum="7194F2799DE0A62F" modified="2022-05-25" version="1">MDRTKHTVGATKRRLIFERSPPPRSPPPLQEITPEPQSQPEKKKKRAYRFRPGTVALREIRKCRKSTDLLIPFAPFVRLVRDIATNYAKDGKPMPWTPHALLALQEAAKYDMVDVFEKAILCLIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B545</accession>
    <name>A0A8I7B545_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
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    </dbReference>
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        <location>
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    </feature>
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        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF08241"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
    <sequence length="256" mass="28257" checksum="FA7E9E2F9BC46D1F" modified="2022-05-25" version="1">MSSSSPPLSPRGAEEEGDTRSDGTGSGGGGTVFSYGEAGYWDARYVEEGGAPYDWYQRYAALRPFVRRFVPPASRLLMIGCGSALMSEDMAADGYMEIINIDISSVLIEMMRKKYFDLPQLQYMQMDVRDMSKFSDESFDCAIDKGTLDSLMCGVEAPLSAAQMVLEVDRLLKPGGVFMLITYGDPSARIPHLNQPVCSWNTVLYILPRPGFKGNTTRSFFDPVPLTKSGRLPEGFVLEDPDSHYIYVCKKGQGQG</sequence>
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                <person name="Brown J.W."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
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            <end position="230"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0133200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24817" checksum="F4513A2D092449C4" modified="2022-05-25" version="1">MPQLYDVYGMAHTASYKKANAFSESDLDDPSNFTNISSHQKLVAYRDAGKATKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLPEIKAHQSSNCPEITRRPRPVELAIEAALQKERVANQAALEKERLASQAALQAALDERDQSTARLLEEERARNEAGQRALYELFVGLCEKSCQVPPPMPVFSSIGTNNSRAASHDPFPGQPSPNEAGASNRGVSPP</sequence>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="257" mass="28923" checksum="AC316536E0537016" modified="2022-05-25" version="1">MPGHPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Antimicrobial_MiAMP1"/>
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    </dbReference>
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        <property type="entry name" value="MiAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303986" evidence="1">
        <location>
            <begin position="16"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="10041" checksum="228B2E6C65A8C94F" modified="2022-05-25" version="1">MAVAMLAAAAGGVAATDGTGPRSYFSSWDGPACTGRMSSAGACGCSPVHFHGGHEFNFRGETATLYSEHGCAGTPYQVFEDTQACGDFGWRSIHIDC</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045123678.1"/>
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    <dbReference type="SMR" id="A0A8I7B7M6"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="42"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="295" mass="32470" checksum="20B8D9D2762DA837" modified="2022-05-25" version="1">MPLSLCRASCVNIGATSARKSSHKTRRMRMEAPADQASGKHIVLVHGACIGGWAWFKVATRLRDAGHRVSTPDLAASGVDPRPLCEVPTFFDYTKPLLDLLESLPPGEKVVLVGHSLGGVNIALACELFPEKVAAAVFLSAFMPDHRSPPSYVLEKFVEGGTLDWMDTEFKPQDPEGKLPTAMQFGPLVTRAKFLQLCSPEDLTLARSLMRVGSMFVEDLRLQPPYTEARYGSVRKVFIVLKDDNAIVEGFQRWMVQNYPVDEVKEIDGADHMALFSTPAELAHCLSDIAVKYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B885</accession>
    <name>A0A8I7B885_HORVV</name>
    <protein>
        <recommendedName>
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        </recommendedName>
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    <organism evidence="6 7">
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        <lineage>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
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    <dbReference type="SMR" id="A0A8I7B885"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0433150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0433150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0433150"/>
    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0359860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0359860.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0359860"/>
    </dbReference>
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    <dbReference type="OMA" id="VGCITIE"/>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="47"/>
        </location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0433150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="164" mass="18247" checksum="1C96C25E16CCDD40" modified="2022-05-25" version="1">MAAGAAAAISSEQMSEFREAFAFFDKDGDGCITAEELSTVIRSLGQTPTPEELRDMVRDVDADGNGTIEFAEFLALMSRKADADADASDPEEELREAFRVFDKDRDGHISKAELRHVMISLGEKLTDEEVEEMIQEADLDGDGLVNFDEFVRMMMLSESDQQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8V7"/>
    <dbReference type="SMR" id="A0A8I7B8V7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221850"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0221850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221850"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="24678" checksum="06FA5DBF77EACA64" modified="2022-05-25" version="1">MEAIVKARKEANEVRKMARNQDAAAEERRLVAEERRVAAEERKVALEERKVGMEERAKLLEWEKHLFFLDTSLFNDAQKEYVNLAREEVLIQKRAMIRTMGDGGLGGMGGGGLDAMGAMGGGVLGGMGGGGGVLGGMGGGGFGAMGGFGAPSNAMGGMGGMSFASLMGGMGAPPAMGGMSFDVPHTHSHENAVEDLAKTVGATRDAVRDDVREEDSSSKAEESSSEEEEDDEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9Q7</accession>
    <name>A0A8I7B9Q7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9Q7"/>
    <dbReference type="SMR" id="A0A8I7B9Q7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0211680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0211680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0211680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0254940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0254940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0254940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF163">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035339185" description="Peroxidase" evidence="18">
        <location>
            <begin position="26"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="31"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="74"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="127"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0254940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="335" mass="35916" checksum="BA1A55723AF3FAEE" modified="2022-05-25" version="1">MATSMGCLVVLCLVSPLLLAGAVHGNPGYGGLFPQFYDHSCPKAKEIVHSVVAQAVARETRMAASLVRLHFHDCFVKGCDASVLLDNSTNIVSEKGSNPNRNSIRGFEVVDEIKVALETACPGTVSCADILALAARDSTILVGGPYWEVPLGRRDSLGASIQGSNNDIPAPNNTLPTIITKFKRLGLNVVDVVALSGGHTIGLSRCTSFRQRLYNQSGNGLADSTLDVSFAAQLRQGCPRSGGDNNLFPLDAVTSTKFDNFYFKNILAGRGLLSSDEVLLTKSAETAALVKAYANDVHLFFQHFAQSMVNMGNISPLTGSKGEIRKNCRRLNNYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAE4</accession>
    <name>A0A8I7BAE4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123444495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044977158.1">
        <property type="nucleotide sequence ID" value="XM_045121223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAE4"/>
    <dbReference type="SMR" id="A0A8I7BAE4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0291360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0291360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0291360"/>
    </dbReference>
    <dbReference type="GeneID" id="123444495"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0242180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0242180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0242180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0291360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0291360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0291360"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444495"/>
    <dbReference type="OrthoDB" id="5577209at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21546">
        <property type="entry name" value="SPOC_FPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000777">
        <property type="entry name" value="Os01g0765300 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052586">
        <property type="entry name" value="ASCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF2">
        <property type="entry name" value="NUCLEIC ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="499"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="663"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="870"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0291360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1108" mass="121205" checksum="E4153C4FF75D2376" modified="2022-05-25" version="1">MTGRQAGELPTSSRHRSIMASSSEPPPKKRKLVDAQTLSPSRTPRLPPPLPPGLSPTPPPPETLAAAAPSAPPPPPQSPPPTPEELHRKRSNREELRKLFECYRRIRLCVERKDARLMPELEHVYLALITASRGCTSVQRILAKLIPQYASYCPTALEAAAKVSIKMYNWNLAIVTRGEDADGVAYRTARACIVGLVDICSTASLEAPKSSVITGICSAVYMTVLTFFISTFDGKDIHHIGSRRLSKLQDPMELIDIVKQESGDDSQLAHDCLFELRALSLLCIFLLSPENLLEACFALIASAETDHIRGLYFLNQLTCNVSKGISDLALDNKADVASQCTEMEIDLSDTKEIVDSKPSSDVSGISGRSMVESNECYITMAISRHPSLRGWILRRYKKICDSCRSTVLSEVSSCLKVLGSLSELDEDKSSMDCEPSVLEKPDKSAGEKTVNSDTYALKSVQTDDVKTEKLADAKTSGRKGARSDLYVASVSSDVISVSKELWVGSLGNSAAEALVRSKFEEFGPLTNFLFYPSKEFALVEYGNIVHAVRACGYMRGSSIWGGDLQIRYLDRLIGSKGFVGGIAVGESCHIYVGKVKNQKEKDEVFDELKSAGLKRPCGCIDISGENALILEFETSVDAAVAKAHIRRQAHSDVYSQDKNTSVHQLLVHNMDKSIPETEFINAFSRFGEVSRWQFNRIDGSCLIDYESQSAAACAKSQMHGARFGLKLISVESRTCSTGAVHDKTFSPVIRMPAQNLPDSSSHHEIRNPRVSAYHTGYTVQGDRPIYGPRPPNTQQLWQYKDLESNRAPQGMPPCPPVSAHRSSVIPPPPPPIQTSFVRPVYPGPGSPWENTTPNPPFGRVSPRMMPGSSFRIPSAPPPFIPSCVTPLTQLPGSSAQHSEKMPPPIPNVAPSPFTPLDMPPPPRSPPPLPVSEPPSVPPPPSSPPVQPSTDPCNSHKPCPHPQWQGSLTKSGIHYCTIYASRIELDACRHESTVPEPAEWPSRLDVLKRTAFQHAKTIISNAPPNKKEVCRLLPCSNGDQKGFRDFISYLKQNKYAGVIKIPPVKPMGSRLLFILPPTSEVFGLLGLPPQQTECLIAVVLPKETATEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCT2</accession>
    <name>A0A8I7BCT2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCT2"/>
    <dbReference type="SMR" id="A0A8I7BCT2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0395370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0395370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0395370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0395370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="22374" checksum="44C2A305DA103DFD" modified="2022-05-25" version="1">MEFFSIILEKPSSRQVLPDNFVKMLDGHRPQNKNLRHAGNGLRKLWDVEVAFDTDGSMYLDRGWKQFVHAYDLRHGYFLGFRYDDNATFAVKVFDTTMCRRRYQDDDDASNGSRSSEYCDRCYVYGSSDSGYSKSSSDSGYNKSSSDDGLAMVIPQLGDRAMPILVEEYNPQPGLGLRRSKRIRMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-07-10" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>A5YTR4</accession>
    <name>A5YTR4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Flavonoid O-methyltransferase</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123445736</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2008" name="Pharm. Biol." volume="46" first="26" last="34">
            <title>Characterization of Two O-Methyltransferase-like Genes in Barley and Maize.</title>
            <authorList>
                <person name="Zhou J.-M."/>
                <person name="Fukushi Y."/>
                <person name="Wollenweber E."/>
                <person name="Ibrahim R.K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Rachis</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABQ58825.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK373043">
        <property type="protein sequence ID" value="BAK04240.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="SMR" id="A5YTR4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330120.1"/>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="COMT-like"/>
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    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
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        <location>
            <begin position="18"/>
            <end position="109"/>
        </location>
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            <end position="338"/>
        </location>
    </feature>
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    </feature>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ABQ58825.1"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330120.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="356" mass="38665" checksum="884125C80FAF9C18" modified="2007-07-10" version="1">MGSIAAGADEDACMYALQLVSSSILPMTLKNAIELGLLETLMSAGGKFLTPAEVAAKLPSTANPEAPDMVDRMLRLLASYKVVSCRTEESKDGRLSRRYGAAPVCKYLTPNEDGVSMSALALMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMSAFEYHGTDPRFNRVFNEGMKNHSIIITKKLLESYKGFEGLGTLVDVGGGVGATVGAIIARYPAVKGINFDLPHVISEAPAFPGVTHIGGDMFQKVPSGDAILMKWILHDWSDEHCATLLKNCYDALPAHGKVVLVECILPVNPEATPEVQGVFHVDMIMLAHNPGGRERYEREFEALAKGAGFAAMKTTYIYANAWAIEFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F2CU79</accession>
    <name>F2CU79_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">indole-3-pyruvate monooxygenase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410027</name>
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    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.168" evidence="3"/>
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        <property type="protein sequence ID" value="BAJ86400.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359334">
        <property type="protein sequence ID" value="BAJ90545.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362932">
        <property type="protein sequence ID" value="BAJ94136.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366668">
        <property type="protein sequence ID" value="BAJ97871.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958834.1">
        <property type="nucleotide sequence ID" value="XM_045102899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CU79"/>
    <dbReference type="SMR" id="F2CU79"/>
    <dbReference type="STRING" id="112509.F2CU79"/>
    <dbReference type="PaxDb" id="4513-MLOC_46161.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644650"/>
    </dbReference>
    <dbReference type="GeneID" id="123410027"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0535520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0535520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0535520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0644650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410027"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_2_1_1"/>
    <dbReference type="OMA" id="RFIYLAT"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CU79">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103075">
        <property type="term" value="F:indole-3-pyruvate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF67">
        <property type="entry name" value="FLAVIN-CONTAINING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ86400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ94136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="399" mass="42855" checksum="3D822432204ECB35" modified="2011-05-31" version="1">MEKSAPPVIIVGAGPSGLAASACLARRGVDIIVLERDDCVGSLWQKRAYDRLHLHLPKQASALPHLPHADDAPAYLPRDHFVRYLDAYADRFAVRARLRLRREVRSARFLDGRWEVEAINLGTGDAERYVARYLVVATGEFDEKVVPEVPGLDTFPGKAIHAGEYRSAEGMRGKEVLVVGCGNSGMEIALDLAQAGAAASIVVRGELHLMTREIMNASTALFAYLPVWMIDRLALFACRIVFGDTARHGLPRPDVGPFTRKIQSNAYPVIDVGTYDKIKSGQIKVLPAMTSIEGDVVEFAGGERHRFDAIVFATGYRSTAKKWLKSDGGGLIGDDGMASGRCPKGENGLYRAGLAGRGIYGSGTDGEFIAEDISRQLLQGSGDDGGDGAITANDGGDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2DA49</accession>
    <name>F2DA49_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123401506</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK360762">
        <property type="protein sequence ID" value="BAJ91970.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951265.1">
        <property type="nucleotide sequence ID" value="XM_045095330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DA49"/>
    <dbReference type="SMR" id="F2DA49"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0629320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0629320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0629320"/>
    </dbReference>
    <dbReference type="GeneID" id="123401506"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0522360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0522360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0522360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0629320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0629320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0629320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401506"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF16">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="97"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="557"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="174"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ91970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0629320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="602" mass="65976" checksum="28B5D9C3D4A0A217" modified="2011-05-31" version="1">MRSLVPEHARHSPGPPPPFSGGVTPPRRRIPKENVYAGAAPPSEDRYRSPSSAAKPLGDVTPTLNRVLPPRPLKRKLDVSPAVGPRPRPEADASDSGVQVVVRIRPPCSVEGEEAPGSCLCKTSTNSVAIQGQSFTFDAVADAASTQEDFFTLVGPPLVENCLSGLNSSIFTYGQTGSGKTYTMWGPLSAISGDSMACERGLAPRVFEHLFSRIKQEQGKHKDKELICSCTCSFLEIYNEQITDLLDPMQKNLQIREDVKTACVYVESLTKESVFTMKDVNQLLVKGLANRRTGSTSANADSSRSHCVFTCVVKSESKNLGDGSNIIRMSRMNLVDLAGSERQKLTHAAGNRLKEAGNINRSLSALGNLINILAEISQSGKQWQHVPYRNSKLTFLLQESLGGNAMLAMICTVSPSESCKSETLSTLRFAQRAKAVKHRTVVNEEKEDDVNALHVQTKLLQDNIADAADWAEAESKRMTLTDELRAELEASKSLVGRLRSELESEKKCSKEAMEAAMQAYSRILVRYADLEGKYILDGVEGQLKDTSEAPGELLVRLKEAEEATRVAEEATRVAERRALLAEQKMDMLIEENVALNQRLTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJ91</accession>
    <name>F2DJ91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine proteinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123443999</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK363959">
        <property type="protein sequence ID" value="BAJ95162.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK368309">
        <property type="protein sequence ID" value="BAJ99512.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976531.1">
        <property type="nucleotide sequence ID" value="XM_045120596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJ91"/>
    <dbReference type="SMR" id="F2DJ91"/>
    <dbReference type="MEROPS" id="I25.051"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069380"/>
    </dbReference>
    <dbReference type="GeneID" id="123443999"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443999"/>
    <dbReference type="HOGENOM" id="CLU_113093_1_3_1"/>
    <dbReference type="OMA" id="WERAWEN"/>
    <dbReference type="OrthoDB" id="1908104at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DJ91">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027214">
        <property type="entry name" value="Cystatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11413">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11413:SF104">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="2 5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="15"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ95162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="107" mass="11800" checksum="E324A444DEB3FEEC" modified="2011-05-31" version="1">MAEAAQGGGLRGRGVLLGGVQDAPAGRENDLETIELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAWENFKQLQEFKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLP0</accession>
    <name>F2DLP0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123446526</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK364808">
        <property type="protein sequence ID" value="BAJ96011.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979034.1">
        <property type="nucleotide sequence ID" value="XM_045123099.1"/>
    </dbReference>
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    <dbReference type="PaxDb" id="4513-MLOC_30704.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380250"/>
    </dbReference>
    <dbReference type="GeneID" id="123446526"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316290.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0380250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446526"/>
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    <dbReference type="OrthoDB" id="1109687at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33132:SF146">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33132">
        <property type="entry name" value="OSJNBB0118P14.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ96011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12075" checksum="393342A2826977C9" modified="2011-05-31" version="1">MDGDSPVMTDGERRAYRYVQAPKVQGLSGMKNSWSNDSLFSYAGAGGSRAVVHSCVCAPTTHPGSFRCKHHRQSAAHLGAAGQAQVQPTAEADADARRNEVHGDMSTADDEKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2DNS8</accession>
    <name>F2DNS8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby-like F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123444087</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="AK365546">
        <property type="protein sequence ID" value="BAJ96749.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976636.1">
        <property type="nucleotide sequence ID" value="XM_045120701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DNS8"/>
    <dbReference type="SMR" id="F2DNS8"/>
    <dbReference type="FunCoup" id="F2DNS8">
        <property type="interactions" value="1223"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2DNS8"/>
    <dbReference type="PaxDb" id="4513-MLOC_64975.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279470"/>
    </dbReference>
    <dbReference type="GeneID" id="123444087"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0231600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0231600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0231600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444087"/>
    <dbReference type="eggNOG" id="KOG2502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028236_3_0_1"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DNS8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF147">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="4">
        <location>
            <begin position="96"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ96749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="356" mass="40118" checksum="E18DFC7E1BDAB601" modified="2011-05-31" version="1">MPCEEPAAEGEVSEVAEMGEEKEDGQPEEQEERWARLLPELLSEVVRRVEASGGERWPARKDVVSCACVCRRWREAAVAVVRPPAVSGKITFPSSLKQPGPKEFPMQCFIKRNKKNSTFYLYLGLTTATVDKGKFLMAARRFRRGPHTEYIISLDADDLSQGSNAYMGKLRSDFWGTNFKVYDSKPPHDGAKASSSRSSRRFGSRRISPQVSAGNYEVGQLSYKYNLLKSRGPRRMYCALEFPSTQESWENSLKTKSRKPMGTKVLRNKAPRWHEHLQCWCLNFHGRVTVASVKNFQLAAATDPNDPSSSRDEGTVLLQFGKVDDDIFTMDYRQPLSAFQAFAISLSSFGTKLACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2DRJ7</accession>
    <name>F2DRJ7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123439464</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="11">subsp. vulgare</strain>
        </source>
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                <person name="Tanaka T."/>
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                <person name="Amano N."/>
                <person name="Kanamori H."/>
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                <person name="Itoh T."/>
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                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        <source>
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        </submittedName>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
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            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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            <strain evidence="4">cv. Morex</strain>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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            <strain evidence="3">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">subsp. vulgare</strain>
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    </evidence>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0427120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0427120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0427120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397526"/>
    <dbReference type="InParanoid" id="M0VUE5"/>
    <dbReference type="OrthoDB" id="597078at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326:SF21">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326">
        <property type="entry name" value="OS05G0543800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3615" evidence="1">
        <location>
            <begin position="27"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0427120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="16658" checksum="50322FD4E05E0288" modified="2013-04-03" version="1">MSLRPKSPPPEELMKREQERNRKSLIVALNTYVKRNNMQITDFEFVEEKERNLVGGVVAGYVHSNFVAKGVDGRPTLFFAEMLHGCFLEEHVILCTPLEDEDSGCCFGCNQHAKKLRHPTCGGYLGGLEDVPFPYVEEDSDDDCFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>M0X6Q3</accession>
    <name>M0X6Q3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979531.1">
        <property type="nucleotide sequence ID" value="XM_045123596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0X6Q3"/>
    <dbReference type="SMR" id="M0X6Q3"/>
    <dbReference type="FunCoup" id="M0X6Q3">
        <property type="interactions" value="1380"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0X6Q3"/>
    <dbReference type="PaxDb" id="4513-MLOC_57462.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402320"/>
    </dbReference>
    <dbReference type="GeneID" id="123447035"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0334880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0334880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0334880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0402320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0402320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0402320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447035"/>
    <dbReference type="eggNOG" id="KOG1558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0X6Q3"/>
    <dbReference type="OMA" id="NTDDHAQ"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0X6Q3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF197">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="350" mass="36943" checksum="19506396812B8E35" modified="2013-04-03" version="1">MAANLKLAALCLLLVVSSLPLLARAECECEAGEEEEHDKAGALRLKIIAIFCILVASAAGCAIPTLGRKFPALSPEKDLFFAIKAFAAGVILATAFVHILPEAFERLGSPCLVDGPWQKFPFAGLVTMLGAIATLVVDTIATGYFQREHAKNSSAAIGNLDPADSEQAHGGHSHGVSAIIASSSCDDGAKLIRHRVISQVLELGIIVHSVIIGMSLGASENAGTIRPLVVALTFHQFFEGIGLGGCIVQARFRHKSFLMMTFFFSLTLPIGVVIGIGIASTYDENSPRALIAEGLLSAAAAGILIYMALVDLLAEDFMNPRVQNNGRLQVIINISLLVGIALMSMLAVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0XY01</accession>
    <name>M0XY01_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123397175</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044947759.1">
        <property type="nucleotide sequence ID" value="XM_045091824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XY01"/>
    <dbReference type="SMR" id="M0XY01"/>
    <dbReference type="PaxDb" id="4513-MLOC_64084.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480320"/>
    </dbReference>
    <dbReference type="GeneID" id="123397175"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0398040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0398040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0398040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397175"/>
    <dbReference type="eggNOG" id="KOG2784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="238316at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XY01">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000033">
        <property type="entry name" value="Phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2330:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase alpha subunit cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF98">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="230"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="501" mass="56886" checksum="0D6F4A3E282B0F8F" modified="2013-04-03" version="1">MAAKQPLADVEDGLLAHLNANAEIPDSRSFASSLGVPHKDVEDVIKRLSAFRIVESADITKETWMLTDEAKGYAARGSPEVQLVAAIPPEGASKGVLRETLGDVFDIGMKAAAKNKWIGFEKGNKDLVLRKVEDTKDELQEQLKRLENGEVIADEVINDLKRRKLVTKEKSIWYSLKKGPEFVVKRKTLATDVTREHLKSGDWKDLEFKDYNYGAQGQPIAIGYSQPLLEVREAIQNIFLEMGFTEMPTNMFVESSFWNFDALFQPQQHPARDSHDTFFLKAPATTTQLPVDYLEKVKQVHQSGGYGSKGYGYDWKRDESEKNLLRTHTTAVSARMLYKLAQEKPFAPKRYYSIDRVFRNEAVDRTHLAEFHQIEGLICDYGLTLGDLIGVLEDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHDGLKKWVEIGNSGMFRPEMLLPMGLPEGVNVIAWGLSLERPTMIMYEIDNIRDLFGPKVDFNVIKNSPICRLGLKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0YU78</accession>
    <name>M0YU78_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398857</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the defense reaction of plants against pathogens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949239.1">
        <property type="nucleotide sequence ID" value="XM_045093304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YU78"/>
    <dbReference type="SMR" id="M0YU78"/>
    <dbReference type="FunCoup" id="M0YU78">
        <property type="interactions" value="826"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0YU78"/>
    <dbReference type="PaxDb" id="4513-MLOC_72965.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519270"/>
    </dbReference>
    <dbReference type="GeneID" id="123398857"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398857"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YU78"/>
    <dbReference type="OMA" id="RTGDCAM"/>
    <dbReference type="OrthoDB" id="745551at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YU78">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000009">
        <property type="entry name" value="Pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="166" mass="17703" checksum="72D4C4A24F570805" modified="2013-04-03" version="1">MEYSPKLAAVLLLALASAMAVTAQNSPQDFVDPHNAARADVGVGPVTWDDNVAAYAQNYAEQRRSDCQLVHSGGQYGENIYGGRGGGADWTAADAVQAWVSEKQYYDHDSNSCSAPADKSCLHYTQVVWRDSTAIGCARVVCDGGDGLFIICSYNPPGNYVGVSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>M0Z6S2</accession>
    <name>M0Z6S2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123427615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967630.1">
        <property type="nucleotide sequence ID" value="XM_045111695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Z6S2"/>
    <dbReference type="SMR" id="M0Z6S2"/>
    <dbReference type="STRING" id="112509.M0Z6S2"/>
    <dbReference type="PaxDb" id="4513-MLOC_77977.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190990"/>
    </dbReference>
    <dbReference type="GeneID" id="123427615"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0158240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0158240.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0158240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0190990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190990.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0190990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427615"/>
    <dbReference type="eggNOG" id="KOG1744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Z6S2"/>
    <dbReference type="OrthoDB" id="582186at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Z6S2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36140">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36140:SF2">
        <property type="entry name" value="OS01G0152950 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190990.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="460" mass="51327" checksum="FD9F3AA6E1F8ED55" modified="2013-04-03" version="1">MAKAARVDAAGELLDDLLLLVFERLPGVQDLLRCAATCKQWLCLITDPVFLRRVGLWPETAPRPSVLVGIFYQNVVPPSAIQPLARIPDSPPQFLSLQAGGAHLTFDSFVSNNDGLFNFAMPLASRRGLLLVGTLLPIPIDSQPREKLHLAVCRPLIDKQGRHLLPPPANREFIIGHRLTGFALLTDEDHRAIDDLDQQRRQPSFQVVLTYSAANRVMYVYTYSSATDTWSTPIECHQVSHLVRCGPCAGVVTCGTVHWLFTDQRSFYTLNVNVATAHVSLTKIPINVHEGEQRRRVPFPCVTRERKLSFVIIREDGVLELWAKQDNDHGGEAGAGGWLRSDLINLGSGDKINLVFFAETRGAMLVEQGGTFFTIDLKSKEKALVDLKGKEMEPVRGICRFPMHRCSSSWCSGFLCSWGKVSPCTYNKPVLYEVVGYLRQDEGRLSQTISKEHVHTSCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287G6S0</accession>
    <name>A0A287G6S0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123445524</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044978453.1">
        <property type="nucleotide sequence ID" value="XM_045122518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287G6S0"/>
    <dbReference type="SMR" id="A0A287G6S0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073480"/>
    </dbReference>
    <dbReference type="GeneID" id="123445524"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445524"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287G6S0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000324">
        <property type="entry name" value="Transcription factor MYB3R-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF61">
        <property type="entry name" value="OS05G0459000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="400"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="444"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="599" mass="65941" checksum="A2701D94BF334FC1" modified="2017-11-22" version="1">MGMAAVKVEERDGCAESRQHLALSSPSVSEGGSYGGGYAWASPVVPSPADSGASRRRTSGPVRRAKGGWTPEEDETLRKAVTVFKGKTWKRVAEFFPDRTEVQCLHRWQKVLNPELIKGPWTQEEDETIIQKVKEHGPTKWSVIARSLHGRIGKQCRERWHNHLDPQIRKEAWTLEEEQVLVNAHRLHGNKWAEIAKLLPGRTDNSIKNHWNSSVRKRLDEYDTGAALPVPVHAAKVPPADNYIDLNKEPNVSLRNHSVIVSHSDPTHSPQVFSLKNIKGCSDFLSLSMPTAQPVTSCQASVADDSAVALAIMGMKMDSARDKGMGLNFVRQKGLQINLLNEKGLGNKLGPSGTAKPDVETDNIGCEPTLVKEAQSFGSLCYQIPKLEDTDLVRSPVLSRHHGSEHGVDGFQLPTGYATPSPTDGRKSDQLSVESILKSAAENFPGTPSILRRRKRDKPTPSQDTDFKIDTNSDGFDTPKGNCTTDSPRSFKTASFLSLGRLDDQRLPSVGKIDVSPAYRLRSKRMAVLKTVEKHLDFSADTMDTCDMVGTLKSSCWNSESINSISDISSVQDKRINGHMVGLETLTSDFAHTTKLDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287LGA7</accession>
    <name>A0A287LGA7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444261</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976862.1">
        <property type="nucleotide sequence ID" value="XM_045120927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287LGA7"/>
    <dbReference type="SMR" id="A0A287LGA7"/>
    <dbReference type="PaxDb" id="4513-MLOC_67295.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284750"/>
    </dbReference>
    <dbReference type="GeneID" id="123444261"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0236190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0236190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0236190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0284750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444261"/>
    <dbReference type="eggNOG" id="KOG2874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="DMKHEDW"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287LGA7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="KRR1 small subunit processome component first KH" evidence="10">
        <location>
            <begin position="58"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="KRR1 small subunit processome component second KH" evidence="11">
        <location>
            <begin position="140"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="44412" checksum="C830DA5451352A5F" modified="2017-11-22" version="1">MASAGEASAAAEATEGKNWRRKGKHEKEKPWDDDPTIDRWTVEKFDPSWNEGGLLEVSSFSTLFPQYREKYLQETWPIVKGALKEFGVSCELNLVEGSMTVTTTRKTKDPYIILKARDLIKLLSRSVPAPQAIKVLNDEMNCDIIKIGSIIRNKERFVKRRERLLGPNLSTLKAIEILTGCYILVQGNTVAAMGSFKGLKQVRRIVEDCIKNIKHPVYHIKELLIKRELAKNPALATESWDRFLPNFKKKNVKQKKPNTKEKKQYTPFPPPQQPSKIDRELESGEYFLSDKVKAAKKYQEKQDKQSEKSEEKRRKREAAFVPPKENTAGLSESAKSSNDNNEIADITKSLKKKAKKFRNSEAEGNVKIESYVASNEESHSKKKRKLSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287SK82</accession>
    <name>A0A287SK82_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287SK82"/>
    <dbReference type="SMR" id="A0A287SK82"/>
    <dbReference type="STRING" id="112509.A0A287SK82"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0430200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0430200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0430200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517660"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287SK82"/>
    <dbReference type="OMA" id="RRWWSIC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009610">
        <property type="term" value="P:response to symbiotic fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000067">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF454">
        <property type="entry name" value="PHYTOCYANIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="16422" checksum="2E83EC6E432AA21F" modified="2017-11-22" version="1">MASKHMLVVAVTTAAVFLPLLAASATLHPVGDGTGWTLGFDYTTWSQSKQFTVGDALVFNYKNAFHNVVEVSGPDFKACKNSKGTIAWTSGADQVHLGDAGRRWFICTVGNHCKMGMKLNVTILAADASSPAPAPAPWAASSLTPHRASRRPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287UTN5</accession>
    <name>A0A287UTN5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123402117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951933.1">
        <property type="nucleotide sequence ID" value="XM_045095998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287UTN5"/>
    <dbReference type="FunCoup" id="A0A287UTN5">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617320"/>
    </dbReference>
    <dbReference type="GeneID" id="123402117"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0617320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0617320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0617320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402117"/>
    <dbReference type="InParanoid" id="A0A287UTN5"/>
    <dbReference type="OMA" id="TGPRECK"/>
    <dbReference type="OrthoDB" id="691053at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0617320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="212" mass="22618" checksum="6F0D88AF283967DA" modified="2017-11-22" version="1">MDDVLLHRSAGGHNFRRRSVGGGGRRLAPMSSPALPPSAYGFFPYPAAPPGPFLYPPYNLYSCGSLPPLPFNHPGLPPRPPATAAKHATFPYQSSPMPPRPAAVTKVKQTAATGMSVSAPRKMLEGKSKKPRTPRSTEEPPRAQRRKPLQRAAPLPATSAVTEALDDLEREVTRGFVEDLLHALAPPPSSLPLPTFSLVRAAATKVPAPCTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WRI7</accession>
    <name>A0A287WRI7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acidic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
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    </evidence>
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    </evidence>
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                <person name="Fiebig A."/>
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                <consortium name="EnsemblPlants"/>
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            <strain evidence="13">subsp. vulgare</strain>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF887">
        <property type="entry name" value="METHYLTRANSFERASE PMT22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0071770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="656" mass="73439" checksum="74365FD1C6935C66" modified="2022-05-25" version="1">MAVPSADRKRRPFLLSLSLFLLLSALLVLLVLFLDSSAQSLPFLPSRLSAPSPHPHQQHSPTPIPTPASSSDSQPDGPAAALVKAEENAPATVAGVSGPSDPSRPVDDSNDAAAVDAAATGVDAAGVDGGEAEDDGAVAEVRWETCKVRRGVSATDYIPCLDNIRAIKALRSRRHMEHRERHCPVPPPRCLVRTPAGYRLPVPWPRSRDMIWYNNVPHPKLVEYKKDQNWVTKSGDYLVFPGGGTQFKDGVARYIQFVEQIMPTIQWGTHTRTVLDVGCGVASFGGYLLDRNVITMSLAPKDEHEAQIQFALERGIPAFLGVIGTQKLPFPDNAFDVVHCARCRVHWYANGGKPLLELNRVLRPGGFFVWSATPVYRKEQRDQDDWNAMVTLTKSMCWRTVVKSEDINGIGVVIYQKPTSNSCYIERKTNEPHLCSKKDGSRFPWYTPLDGCILPSAVSSSDETSNSPRLWPERLVRYASVPDDSATIEKFDADTKYWKQVISEVYYRDFPVNWSNVRNVMDMNAGYGGFAAALVDQPLWVMNVVPIGQSDTLPVIFSRGLIGVYHDWCESFNTYPRTYDLLHMSDLLGSLTKRCDILEVAAEVDRILRPGRLFVLKDTMDMIKKMRPVLRSLHYETVVVKRQYLVATKTFWRPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM04</accession>
    <name>A0A8I6WM04_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123431360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971104.1">
        <property type="nucleotide sequence ID" value="XM_045115169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WM04"/>
    <dbReference type="SMR" id="A0A8I6WM04"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0216930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0216930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0216930"/>
    </dbReference>
    <dbReference type="GeneID" id="123431360"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0180370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0180370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0180370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0216930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0216930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0216930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431360"/>
    <dbReference type="OrthoDB" id="245563at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1300.10:FF:000004">
        <property type="entry name" value="Outer envelope protein 64, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310:SF7">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0216930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="47091" checksum="AE952D89059FBE3D" modified="2022-05-25" version="1">MSQPRAMEDAAGDDHGAFIQKFILLPSSDHHQQQEQLPLHGLTFAIKDIFDVAGRVTGFGTPDWARTHAPAATTAPAVLAALAAGATGVGMTVMDEMAYSINGENAHYGTPANPCAPGRVPGGSSSGSAVAVAASLADFALGTDTGGSVRVPAAYCGIFGLRPSHGVVSAENVVPMAQMFDTVGWFARDVATLSRVSDVLLPPVSTDAELPRPSRVIIPADCFKILGSSDDDHTYEILNACAAKVFGSDAVVDNGDLGDFVSSNVPRIGKFMTDLSAIEASSSCVPSLSAISRVMRVLQRSEFKANHAEWVNTVKPNLGPGIRERVQEAIASEDRSAMDDLHAVRTEFKSALAALLKDDGILAIPTVPGAPPKLRMDAALLEDFRARAFSLLSIAGLSGFCQVSIPLGTRHGVPVSVSLLAGHGGDRFLVAVAQELQEALQAEAAAAWGSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMA0</accession>
    <name>A0A8I6WMA0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123418261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family. B11E subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962912.1">
        <property type="nucleotide sequence ID" value="XM_045106977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMA0"/>
    <dbReference type="SMR" id="A0A8I6WMA0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083770"/>
    </dbReference>
    <dbReference type="GeneID" id="123418261"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0068820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0068820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0068820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083770.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123418261"/>
    <dbReference type="OrthoDB" id="665742at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01959">
        <property type="entry name" value="nsLTP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF74">
        <property type="entry name" value="OS05G0550600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152936" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="4">
        <location>
            <begin position="30"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083770.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="98" mass="10006" checksum="10C55E7C88C2AB91" modified="2022-05-25" version="1">MAASKATLVCFMIVALAAALLAAPGTVEAATCSPTQLTPCAPAIIGNAAPSAACCGKLKAHPASCLCKYKKDPNLQRYVNSPNGKKVFAACKARLPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSP3</accession>
    <name>A0A8I6WSP3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123446591</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="RefSeq" id="XP_044979106.1">
        <property type="nucleotide sequence ID" value="XM_045123171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSP3"/>
    <dbReference type="SMR" id="A0A8I6WSP3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0075780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0075780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0075780"/>
    </dbReference>
    <dbReference type="GeneID" id="123446591"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0061370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0061370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0061370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0075780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0075780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0075780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446591"/>
    <dbReference type="OMA" id="MATTWER"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="136"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="350"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0075780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="431" mass="48276" checksum="4006A9E1C8AD9D99" modified="2022-05-25" version="1">MAQPQQRVYQAWKGNNRFFLGGRLIFGPDAKSLLVSVALIVVPVLVFCAFIAPHLLHRFPDYNAGYAIPAVAVGFMIYVLLLLLITSAQDPGIVPRASHPPEEDFAYGNPLAGETPGRLQFPRIKEVMVNGMLVKIKYCDTCMIYRPPRCSHCSICNNCVERFDHHCPWVGQCIGQRNYRYFFLFVSSSTLLCIYVFAMSALHIKFLMGEDYPTVWKAFKHSPACLVLMIYCFIALWFVGGLTGFHSYLISTNQTTYENFRYRADSRPNVYDQGCLNNFLGVLCSKGKPSKHRFRAYVQEEVRAPVVNFGRQMEEEAAGGPRAKVEDDLEIGSDLLKISQRRNYEDVDVEMGNQDDSETKGMGSAKPKPAMGSGSQIPAVGSEVRVRHSSWDRRSGNWDMSSEVIGRSASDVLGRSASVTEAASQSRREAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSR7</accession>
    <name>A0A8I6WSR7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein cereblon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein ohgata</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123446851</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Has an essential role in mediating growth by negatively regulating insulin signaling. It also has a role in maintaining presynaptic function in the neuromuscular junction synapses of third-instar larvae.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Likely a component of a DCX (DDB1-CUL4-X-box) protein ligase complex. May interact with pic/DDB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRBN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979326.1">
        <property type="nucleotide sequence ID" value="XM_045123391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSR7"/>
    <dbReference type="SMR" id="A0A8I6WSR7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076780"/>
    </dbReference>
    <dbReference type="GeneID" id="123446851"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0062240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0062240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0062240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446851"/>
    <dbReference type="OMA" id="IGECANC"/>
    <dbReference type="OrthoDB" id="267517at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000005">
        <property type="entry name" value="Blast:Protein cereblon homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000007">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732:SF8">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="13">
        <location>
            <begin position="100"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="14">
        <location>
            <begin position="404"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="250"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
    <sequence length="519" mass="58135" checksum="A9D7DADBACAE9641" modified="2022-05-25" version="1">MEADWDRMVLQVDMEDLERAELDALVVDEHRHERFEREWEQMEDQIYRQVLAIGSFARLDDGGGRGEFGHQTALHTYLGEVDDIPGRKATLLDAGSIISLPMLFLHGVVLFPGATLPLKLIEARFVGAVEKALRHVDAPETIGVVLMHGRPNHRNYANASVGTTAEIRQLGRSEDGSVNVKARGQQRFRLIRYWADVDGVVWGEVQIIEEDPPLRTPRAAFAQLGASRSYRPHTSSQVMSLDVSPIKQQGHIYSDPDCDTPTSSDSSSQSSNSVKPYVSGQSGKNTNMDEEEYICSTPSSARTHVKHQYNAGNYSKQPLQASLAFWPQWAYEMHDPYALACRAADLWRLIIKTPNVDELVRKPDLLSFYIGNQLPVSEPVRQKLLEINGVSYRLRKEIQLLKACDLIKCGYCKSEIAKHSDVVVMSSDGPLGTYVNNHGYVHATITVNNATGLDIEGDPTEEHSWFPGYAWTIASCSSCDSNIGWLFTATKRYLHPKFFWGIRSCQIADIVDAGQQQEE</sequence>
</entry>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        </source>
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</entry>
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                <person name="Muehlbauer G.J."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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    </evidence>
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        <source>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="807"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="840"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="872"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1298"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1323"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1410"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1447"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1518"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1537"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1561"/>
            <end position="1570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1590" mass="169989" checksum="8D7A8CF78481B55E" modified="2022-05-25" version="1">MAAPGGPPPPPPPPPPPPPKSDQAVGDASASPPPPPDTETAGQTAQTSPPPPSPKSDQAVGDASATPPPPPETETAGQTPPPPPSPKSDQAVGDASASPPPPPDTERAGQTPPPPPSPKSDQAVGDASASPPPPPDTERAGQTPPPPPSRKSDQAVGDASASPPPPPDTETAGQTPPPPPSRKSDQAVGEASASPPPPPDTERAGQTPPPPPSPKSDQAVGEASASPPPPPDTERAGQTPPPPPSPKSDQAVGEASASPPPPPDTERAGQTPPPPPSPKSDQAVGEASASPPPPPDTERAGQTPPPPPSPKSDQAVGEASASPPPPPDTERAGQTPPPPPQNQTQAAETASQPQADTQRQTGTGEVAGMPEITQAAPSQETTAAAGAEDHLPAPPPPPEEQKQGETSKDGKDSVRAEEKKPGASHWPRVRSPVLLLLSLFRLRRHGKQSMPIEQPKVSPSAPEDELAAVRKEESDRSKRRRHEAEASSSPAGRKEAPPAEPHQTKRRKSLQRTESVRPPEGVAAAAGEADATPSPMRKKLQKAIVLVKKTLSWYGRHHSPENAEKPEEEEDQSAARKEKGGDTKPSTSPTPPEEAGTKSKQHDKTEEEEKKAKEEAEKATATAKEDARLRLWARAEVRLERILEEACTRLTLAEFMKLGKPATNNDPDDKRQECLLTFSVFPLGSQVKKQAVTYWWCTRFNLSSEKGRGSADETFSKLSAGGFLEAFKNRCSGVIYGCRVNPLVHWMVKHVAREKECADLEEPGGPAFSQPKSDVLCLTAGNRARMQMTKVTPPNKEAVGEPSQSQKLHEDGDSKAQLKPNSTKEPSGGPQAVGHPSQKLPEDGDSKAQLKPDTTKDPPGDTIAAVGHPTQKLHEDGDSHDQLKPSPTKPPGGTIAVGRVSPKNCFCAPRPQDKQDKTDDDDETLEIAKLVSEFKKKKVILNINAHVYRLPELLLTQLGDGLEVLQLGRWGNTDDETYMEVESLDTLGAIGNLKNLRYLSVRGLSRLTELPAEVRRLRRLAILDVRGCQNLVKLPSSTVKKLVGLTHLDLTECYMLEHVGRGVAALPELRVFKGFVFGVGKRRYDACRLQHLAKLKKLQKLSVNVTTDANVEKDEMKQLGRLAALVSLTVTWGEKPSILLHDSEKVRKQLEGLLNTWTGLRLPPALEKLDVRCYPKDKLPLGTWLNGNKKLKKLYVRGGEVEDLEIPQDNAIETLRLRYLQKFEMGWSELLAKFNKDIIKCVEVVDKDYKVTRAQTKGKDIVEAKVVDKVESDGTKKEMTPIKKRMKIPPCTVDEDGVWVRDPKEDTGDQTPGKAEAATDVAGKAEKGDDGASKAQKEVKEEEKPSSLEGTHGEKSAPAKDDNAATDEIKKVDSGDKNHETVNKESDNKREAKDEERDPIKNGAQVQESTIDKNKLKVRDEELKEALEKKPSPTLADDQTVAPASDGDIKKGADDKTDQNKISETKEERQDKSGGAKGQDESPTKEDTGGDTNEPQTEGEDKGKDDEQPDEGGGDNGGDASVGDVMSSEASEKTKEATTATLPPAPETTTSPTATPPALPPATTSAAPAAVDSKEPGKAGQEQAPDVENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X391</accession>
    <name>A0A8I6X391_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein farnesyltransferase subunit beta</fullName>
            <shortName evidence="10">FTase-beta</shortName>
            <ecNumber evidence="2 10">2.5.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123444351</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteinyl-[protein] + (2E,6E)-farnesyl diphosphate = S-(2E,6E)-farnesyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11535"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:86019"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.58"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of FTA and FTB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="2 10"/>
    <dbReference type="RefSeq" id="XP_044976976.1">
        <property type="nucleotide sequence ID" value="XM_045121041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X391"/>
    <dbReference type="SMR" id="A0A8I6X391"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287390"/>
    </dbReference>
    <dbReference type="GeneID" id="123444351"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0238600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0238600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0238600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0287390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444351"/>
    <dbReference type="OrthoDB" id="10261146at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02893">
        <property type="entry name" value="FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000017">
        <property type="entry name" value="Protein farnesyltransferase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026872">
        <property type="entry name" value="FTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="101"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="490" mass="53368" checksum="E402D001A25619F2" modified="2022-05-25" version="1">MVRLSLRSSWPQGDGARSRGTQPSDGQPPPASAAPMDSSSRTQPQPPPAGGDPSEDESAAELPRLTVTQVEQMKVEARVADIYRVLFDAAPNAKSAVLELWRDQHVEYLTKGLRHLAPSFHVLDANRPWLCYWMVHGLALLDETLDDDLENDIVDFLSRCQDKHGGYGGGPGQLPHLATSYAAVNTLVTIGSEKALSSIKRENLYKFMLLMKDKSGAFRMHDGGEVDVRACYTAISVASLVNVLDDKLARGVGNYIASCQTYEGGIAGEPSAEAHGGYTFCGLAAMVLLNEVEKLDLPSLIGWVAFRQGVECGFQGRTNKLVDGCYSFWQGAAIALAQKLMTVVAEQSKPSYSSKLSSVDDACGTSSSGLASEKSPIVDYVKFGFDFMKQSNQIGPLFHNIALQQYILLCAQVLEGGLRDKPGKNRDHYHSCYCLSGLSVSQYSAMTGSDSCPLPQHMLGPYSNLLEPIHPLYNVVLEKYEEAYEFFSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X497</accession>
    <name>A0A8I6X497_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Cysteine synthase</fullName>
            <ecNumber evidence="11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123430277</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="11"/>
    <dbReference type="RefSeq" id="XP_044970087.1">
        <property type="nucleotide sequence ID" value="XM_045114152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X497"/>
    <dbReference type="SMR" id="A0A8I6X497"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0167970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0167970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0167970"/>
    </dbReference>
    <dbReference type="GeneID" id="123430277"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000130">
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
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        <property type="entry name" value="CysK"/>
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    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00291">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0198">Cysteine biosynthesis</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="12">
        <location>
            <begin position="14"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="186"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
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    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0167970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="327" mass="34465" checksum="6723C366FB3CD2D3" modified="2022-05-25" version="1">MGQWCPSPTIAKDVTDLIGRTPLVYLNKVVAGCEARVAAKLEIMGPCSSIKDRIGYSMIADAEEKGLITPGKSVLIEPTGGNTGIGLAFMAAAKGYKLIVTMPASVSTERRTVLKAFGAEVVLTDPLRAMDDVVRRAEEIAAKTPNSYVLQQFENPANPKVHYETTGPEIWSATAGAVDILVAGIGTGGTITGAGKYLKEMNPEIQIYGVEPSESAVLSGGKPGPHKIQGLGAGFVPGVLDVNILDEVFQITNEEAASMARQIALKEGLLVGMSSGATAVAAIRVAQRVENRGKLIVVVFASCGERYLSSFLFESIRNEAGNMVLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X5G4</accession>
    <name>A0A8I6X5G4_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="34"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179740.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="193" mass="20951" checksum="12F618402423B28D" modified="2022-05-25" version="1">MTGLKPSVNLFALLDRNDPGDKLVTDFDDTNAKQEATAAKHKKKPTPAADHKDLLSQAYPSARDYVIRKQQRERQARAKAKAEEEARAKAKAKASNGVSGDDKSRGTSTDSGKVQDAAKQGRYYNNNNYNEASRNQQFGMSGRFGAPKRHGGINNNNNEAAPAEDVEAPPAPQQPPPPPPPSLDDISEFPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X867</accession>
    <name>A0A8I6X867_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123445708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X867"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0074020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0074020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0074020"/>
    </dbReference>
    <dbReference type="GeneID" id="123445708"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0074020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0074020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0074020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445708"/>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33356:SF12">
        <property type="entry name" value="ATAXIN-2 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33356">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0074020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="361" mass="38255" checksum="8F31395A44C971CC" modified="2022-05-25" version="1">MAVEWSDGGEEFLLPDEFLDDDFFSEEEKAAVAARSESDEEDSLAGLARRLASLLAGDGLPAPTAKEEVVAGSPKSTLCGLPKSGQESPNSGASQGNSPPSSPLEQQPADPWDVLYEAEGHAARMRVNSPIPVPGNPYGLHGRGMFVPPARKASPPHAPAPAGTAAGGVYYPPFAHLVTQRQIHAARFHLLKQQQLFKQQRDRQLAAAAAWSVRRNAEAKRAGAGPIDLSPASFPPLLKTHLQHTPAPRPTHPPAAGMRAVFLTPPGAKRERAGTGVFLPRPAGFPAEPKKKPSCSTVLVPARVVHALNLNLDDLGSQPRYPGGFVLDHDALISRSNAMLASHKMRAAETSAPHREPAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X895</accession>
    <name>A0A8I6X895_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="272"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0120980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="517" mass="56451" checksum="C31BE388ED73496C" modified="2022-05-25" version="1">MDSAEYVFDSRESEEEDSEEEEEDEEHDSPPPPPEQRSKSRHEPVIPSAPPGLSSALPTAPAVPSARGTKRSRDAPAEPAGRSPRAPKPSGPKPRRALPRMRVAIPVTSTVATSTTSPVRQGDDPMDTDNVVSSQPGAIHPDEDGQGRAEAAAEPVVEPILEATPPATAPAADVPPTEVPPPTEPVPVGDELTGANLGTPQDLPTIPGSSNVEFSIRCVPEEQVGAAKGAMIQAELMTGEAKKAYDSVAALYQRSLELRDDIRKTCDMGSAYEALKAEKNQYAGELEAATLAMAGLKNALEVREKSLEEPLEANRALVAEVERLKKQRTEVYDQMAAQNKRWTAQEKYVNDWAVQMMSRLAAEAAAVERSMKDNVPLSEDANRNLLRAHIRVGKVGPFINRLREIFGRIEKELWPEDESREKMETMLGRLREIPARVQSWKKSAARCGADVALSLVRVHRKEVREDKLKALKVANTKKLQFEDFMETFLETATRIADGIDLDTFVEPSSPDAGPADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIB6</accession>
    <name>A0A8I6XIB6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123440721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973205.1">
        <property type="nucleotide sequence ID" value="XM_045117270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIB6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311550"/>
    </dbReference>
    <dbReference type="GeneID" id="123440721"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0260190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0260190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0260190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0311550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440721"/>
    <dbReference type="OrthoDB" id="660486at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
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        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF118">
        <property type="entry name" value="OS01G0924966 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311550.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17159" checksum="E4D3A57B964CFE6B" modified="2022-05-25" version="1">MAASKGARKSLVSRTLERCRSGLNSGGRSSAAVAPGCFSVYVGPERERFVVRADRANHPLFRRLLDDAEQEYGYAAQGPLALPCSVDAFLDVLWHMDHDVHDDDEDGEAAVAPWTPICGLQRGGSGNIKVRPAGYRVLSPPKSTPPSFFFSQTAAGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKU1</accession>
    <name>A0A8I6XKU1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123447244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979764.1">
        <property type="nucleotide sequence ID" value="XM_045123829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKU1"/>
    <dbReference type="SMR" id="A0A8I6XKU1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408280"/>
    </dbReference>
    <dbReference type="GeneID" id="123447244"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339920.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0408280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447244"/>
    <dbReference type="OrthoDB" id="668748at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048830">
        <property type="term" value="P:adventitious root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010311">
        <property type="term" value="P:lateral root formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529">
        <property type="entry name" value="LOB DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529:SF66">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="50"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408280.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="284" mass="30410" checksum="989DF5CB1735636B" modified="2022-05-25" version="1">MALGRPLHPNHPQQQQQRSPQHRRQQRSGRPPSDPAVITDRLMSMTGLGSPCGACKFLRRKCARGCVFAPYFCHEQGAAHFAAIHKVFGASNVSKLLAHLPISDRAEAAVTVSYEAQARLRDPVYGCVAHIFALQQQVMTLQAQLASLKAHAPPAPQGMQQHQDDVKGYVGGGAADQYGHGAYQWYNGNGAAAQQQCAYGGNGGAVAGHDSITALLSGSAASDYMMYHALEQSASDDDRHAAAAAFEAADQSSFGTEESGWRSSSGYQDCEDLQSVAYAYLNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM09</accession>
    <name>A0A8I6XM09_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979196.1">
        <property type="nucleotide sequence ID" value="XM_045123261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XM09"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392220"/>
    </dbReference>
    <dbReference type="GeneID" id="123446704"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0326280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0326280.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0326280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0392220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446704"/>
    <dbReference type="OrthoDB" id="642536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF57">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="68"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="402" mass="45535" checksum="690535EA49DFF8A4" modified="2022-05-25" version="1">MSSPTRRRAHVPRWSSIPTELAGVVLRRLPSHANRVRFAAVCKQWRASALQSSPPSHYPWLALPDRTFYSLPGSAFRPLPLHLDRHRQLPHAHSSCGEWLVFERYDGAYTLVSPFSMSTTILLPGLSDTYAPNVPLLVANDQPKPNMLKLVVCSRDLVAAIINDEGWMYNKLALCRPGASSWRSSTPDQLCDLQDIVSCQGKLYALDKFNGLFSVSISVFGNMRTGSGEPIVSQVEHLLYRPRGVLRHSSRYLLESSGTLLLVCREDAMPEQCSSTSMSAVMDGLELEMEMEFELLKADLERSRWTNVSSVGDDRVLFVGPWCSRVLHVTGEHDPIYTAGNRIFFTVSAKRYNRCQYYGKQEPFYCIVYDMRTQRSELFLKTPVRPLLMDFPVTWLFPPSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XN35</accession>
    <name>A0A8I6XN35_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123398112</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044948550.1">
        <property type="nucleotide sequence ID" value="XM_045092615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XN35"/>
    <dbReference type="SMR" id="A0A8I6XN35"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462450"/>
    </dbReference>
    <dbReference type="GeneID" id="123398112"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0382820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0382820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0382820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0462450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF320">
        <property type="entry name" value="S-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="152"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="387"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="421"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="465" mass="52190" checksum="6C84773D1B3F133C" modified="2022-05-25" version="1">MMVSSEAEPSPPQQPKRLYQAWKGNNVFLCGGRLILGPDAASLLLSSFLVAGPAIVFCYQMQSKFFRSNGQPHMHRAALLIVIITTLVDLFFLFMTSARDPGIVPRNTRAPPPEADERNLPATPSMEWSVGGTPRMRFRRTKDVNVNGFTVKLKFCETCLRYRPPRSSHCSICNNCVQKFDHHCPWVGQCIGLRNYRYFFLFIATSTFLCISVLIFSWLNVHCEMQDNGGSIWKALRKEIYSFVLIIYTSIVVWFVGGLTVLHLYLISTNQTTYENFRYNYDKKDNPYRKSITKNFAEVFFTKIPPPLNDFRSHVGEGALEAGFYTPYIGLDVASTREKIDTDMREKEVLVAGVQIPTVLQNIDYGSFEDGLYDKNRNNGNKTVAFAPAWSQKGSEDAGTSAAATTSCKEETSEDDAKEISNSNTSSARTSTEANTISEVEIVKDDAKESNTPDRSSARSLKGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XND7</accession>
    <name>A0A8I6XND7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123397297</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947838.1">
        <property type="nucleotide sequence ID" value="XM_045091903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XND7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480410"/>
    </dbReference>
    <dbReference type="GeneID" id="123397297"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0398130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0398130.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0398130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480410.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397297"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0186">Copper</keyword>
    <keyword evidence="4 9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480410.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="153" mass="15956" checksum="F8EA17DC2A4BE925" modified="2022-05-25" version="1">MMHMTFYWGTSATILFDGWRTSAWTGYLLSLLALFLAAAFYQYLEAFRIRVKLLAGAKADPLPPPAGSDARAPLLAPGAAAFAGGRMPARVATAALFGVNAGIGYLLMLAVMSFNGGVFIAVVLGLAAGYLAFRSGDVDGDDLVVVDNPCACA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPN6</accession>
    <name>A0A8I6XPN6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444608</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977323.1">
        <property type="nucleotide sequence ID" value="XM_045121388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPN6"/>
    <dbReference type="SMR" id="A0A8I6XPN6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294720"/>
    </dbReference>
    <dbReference type="GeneID" id="123444608"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0245260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0245260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0245260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0294720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0294720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0294720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444608"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298135" description="Subtilisin-like protease" evidence="8">
        <location>
            <begin position="28"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="139"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="634"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0294720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="79259" checksum="EFECAC7D75240941" modified="2022-05-25" version="1">MDFHSSPRRFSAYLLLCLCLVISISRGYGSQKLYIVYLGEKKHDDASLVTASHHDMLSAILGSKQEALASITYSYKHGFSGFAAMLTDDQAQDLAELPEVISVTPNQNHDLMTTRSWDFLGMNLDHQPPNKLLQRSKYGEDVIIGIVDTGIWPESRSFSDDGYGPIPSRWKGVCQLGQAWGSTNCSRKIIGARYYPAGLDKADQANNYMSARDINGHGTHTASTAAGAIVEGVSLHGLAAGVARGGAPRARLAVYKVAFEGPKKVQLASAALLAALDDAIHDGVDILSLSVVYNDNSFGSLHAVQKGITVVYGAGNSGPRPQVMSNTAPWAITVATSKIDRSFPTAITLGNNQTIVGQSLYYMLKNESKSEFQPLVEGGSCSVEALNGTEINGKVVLCIKETFGPPADIIPDAITNVKTGGASGLIFAIYTFDKLLSTEDCVGMACVIVDIDIGYQVATYIGSQGSPIAKIEPASTITGVRVPAPRVAFFSSRGPSVKHPTVLKPDIAAPGVNILAATGDGYVFDSGASMSTPHVAGIVALLKAVHPDWSHAALKSAIVTTASTKDEHGMPMLAEALPRKVADPFDYGGGNINPNAAADPGLVYDIDPRDYSKFFACTIQKYEICNISTSPAYQLNLPSISIPELRGPIKVQRAVTNVGEVDAVYRADIQSPPGVKIKVDPPTLVFNATKKVHAFKVSMTPLWKVQGDYTFGSLTWRNEHHSVRIPLAVRITIQDFYADVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XR43</accession>
    <name>A0A8I6XR43_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ALOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123440575</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant homeotic and developmental regulators ALOG protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973074.1">
        <property type="nucleotide sequence ID" value="XM_045117139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XR43"/>
    <dbReference type="SMR" id="A0A8I6XR43"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304120"/>
    </dbReference>
    <dbReference type="GeneID" id="123440575"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0253830.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0253830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0304120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0304120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0304120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440575"/>
    <dbReference type="OrthoDB" id="1906822at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009299">
        <property type="term" value="P:mRNA transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040222">
        <property type="entry name" value="ALOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006936">
        <property type="entry name" value="ALOG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165">
        <property type="entry name" value="PROTEIN G1-LIKE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165:SF78">
        <property type="entry name" value="PROTEIN G1-LIKE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04852">
        <property type="entry name" value="ALOG_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51697">
        <property type="entry name" value="ALOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="31"/>
            <end position="158"/>
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            <end position="212"/>
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            <begin position="1"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51697"/>
        </source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0304120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="212" mass="22164" checksum="7124E350B53F9BD7" modified="2022-05-25" version="1">MDSSGPGTSSSAVVGGGAPDVAPKPPAQLSRYESQKRRDWNTFLQYLRNHKPPLTLARCSGAHVIEFLRYLDQFGKTKVHAVGCAYYGQPSPPGPCPCPLRQAWGSLDALIGRLRAAYEESGGAPESNPFAARAVRIYLREVRDSHAKARGIPHEKKKRKRAPQAAEASGSSAGTAAVFGRDVEGSAGDGALAASAAQAGARDGGSTAPSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRJ3</accession>
    <name>A0A8I6XRJ3_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="347" mass="39721" checksum="27A5D40AFBA2EC6D" modified="2022-05-25" version="1">MKAYLIELLRAHDVPKYRTQNAWSKEAWTSIVGKFNQRFDLSFTVIQVKQKEQDLKRDFKAIKDLISESGFGWDHDRKMVVAPDNVWAALEARKNKDALTWRGRSFPYYEDLFALYDGRYAQGRSCHGMDYYANKATQPSQFPASHSPQLQGLEAHLHTPTPTIHAPGDSSMQFHIEEDSENTKWLSSNNTFSQVEVNSTQGNDLTLHTPQVEAIPISSQHVGQTLHENPQVAHRNPRPSSSGPEANSTRRAKKQKTTSIDDFHERYLKLRREEIDRYAAIEERKLKDPFSIKKCIKALERLEGLSMADMLKAADIFAANKENREVFLSFSSNALRLGWLIGKVQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS25</accession>
    <name>A0A8I6XS25_HORVV</name>
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        <recommendedName>
            <fullName evidence="12">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS25"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308740.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
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        <property type="entry name" value="GNAT_dom"/>
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        <property type="entry name" value="IDM1_C"/>
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        <property type="entry name" value="TDBD"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF21">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASE WITH RING_FYVE_PHD-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_PHD_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="356"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="496"/>
            <end position="641"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="203"/>
        </location>
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        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="697" mass="74615" checksum="8988E76B5C259BE5" modified="2022-05-25" version="1">MQSNGRTPPLFSPGSGPGMPPLAVYSRKQGASTAGGPAAVQRDAGGNQLPSGTDSGHGKPASEMLLQIVPANTGAGTDENVPPANNGRVLPMPMTRVAFKVKAAARSPNGRPRKARAPKGSSTLAGSREATGKKSDAGEPVGIHRGAQGNDQPKTVSAPSNSRKRKSTASASALSSSRRPRRTRCSLVARRNNSSTSTSGTAAARAKKHTILTWLIDSGVLKENEKVWYVPGPDSTSVAARASGAVTRAGIQCSCCDAVMAPPAFSSHAGFEDSAPWERLLVESGKLLLRCVREAWDQERLRTLRAEEKARAALEQGRERSAQAKKRSLLLTRQIRKAPTLAVDGTNNGGDDDRSDDVCGVCADGGQLLCCDSCPSTFHPECLAVEVPEDSSWVCHYCRCFLCDDGHGSLSTCRQCARNYHHHCRASVLDGHEIAPYCSKACNKIAVNLSNMVGGMISTGEDGYSWSLLKIQRDSGSSDPAALLKCNAKLAVALGVLDECFNPVKDRLTGIDMLHQAVYSLGSEFKRLRYEGFYTMVLEKDTEIISVALLRLHGTKLAEMPFAGTLPQYHRQGMMRRLVNAIDKVLESVQVENLVISAVDEAVDTWKKFGFVPVDPQLRDEAKRLSMVTIAGTILLQKPIVQQQVLPITEDELAFMEMRWPLCSFVDLLTGIASPQPPYADPIAAAVRGAGGGSGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUB6</accession>
    <name>A0A8I6XUB6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein IRON-RELATED TRANSCRIPTION FACTOR 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123445544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination. Promotes the accumulation of mugineic acid family phytosiderophores (MAs). Required for ethylene-mediated signaling during iron deficiency responses. Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978475.1">
        <property type="nucleotide sequence ID" value="XM_045122540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUB6"/>
    <dbReference type="SMR" id="A0A8I6XUB6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321630"/>
    </dbReference>
    <dbReference type="GeneID" id="123445544"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445544"/>
    <dbReference type="OrthoDB" id="6106870at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000074">
        <property type="entry name" value="Transcription factor ORG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF156">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="69"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="246" mass="27002" checksum="C192835C01A179B7" modified="2022-05-25" version="1">MGHQTQMFDDPFASSMSSLDADIFSVAGGLHPSQWPGLDHDVSLAPAANNGTSSGGYGSPGGGDGSGSHRKISHNAYERDRRKQLNELYSDLRSLLPDSDHTKKLSIPITVSRVLKYIPELQKQVDGLEKKKEELTRASCKPGVLTMKENTVPIVSATCLDEREIMVQVSLVSTMAGALPMSKCIKVLENEGLRLISSSTSAFQNRTFYSLHLQRTQRTMSKECPAFCEELENALTQKAGLRLHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUG3</accession>
    <name>A0A8I6XUG3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUG3"/>
    <dbReference type="SMR" id="A0A8I6XUG3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0432190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0432190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0432190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000108">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000002">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000004">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF60">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="328" mass="37119" checksum="02A4DE8B1E1AD92F" modified="2022-05-25" version="1">MNRFNQTPWSKVQCEMILAFLSFAEYFRPRFFLLENVRNFVSFNKGQTFRLTLASLLEMGYQVRFGILEAGAYGVAQSRKRAFIWAAAPGETLPDWPDPMHFFASPELKINLPRGKYYATARSTAGGAPFRPMTVRDTIGDLPPVENGTSKPTMQYGNEPISWFQKKIRGDVLSLSDHKSKEMNQLNLIRCKHIPKRPGCDWHDLPDEKVKLSTGQTLELIPWCLPNTAKRNNQWKGLYGRLDWEGNFPTSVTDPQPMCKVGMCFHPDQDRIITVRECARSQGLLDGHRFAGNIQSKHRQIGNAVPPPLASALGRKLKQAIDAKPTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUI4</accession>
    <name>A0A8I6XUI4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123443824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976285.1">
        <property type="nucleotide sequence ID" value="XM_045120350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUI4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0269680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0269680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0269680"/>
    </dbReference>
    <dbReference type="GeneID" id="123443824"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0223490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0223490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0223490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0269680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0269680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0269680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443824"/>
    <dbReference type="OrthoDB" id="629734at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141:SF159">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="363"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0269680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="456" mass="50629" checksum="F7568E62B78C42ED" modified="2022-05-25" version="1">MDETLATVLSYLPPPSVSTTVSPSSSSATSDEDDRINYLHDVLLRNIISRLPTKDAARTTILSSRWRSLWASTPLRLDDAGLVPTAVTAALNSHPGPVTSARLSSEHLAYQEPDVLDSWFASLAAKNVEELSVVNGSWPAECEWRPPPGALGCAALRRLWLGLCQFPDMSGLTPAFPSLQELGIVHCSMQDRELHALLPRCPELESIAFVLTQDYPRYIHIWSGSLQSLVVWRSMVREVHLDDAPNLERLLLEPIGGASTHVKIINAPRLKIFGYFDVGLHQLKIGPTVIKDGIGMKVKPNAMVRTLRTLALNVQFGDKKQVKLVPLLLRCFPCLQTLYIKSSPSESPTDVDVDFWDQVGHTECVTSHIKKFVFEAARGEDTELAFVKFVMERAQMLEEMRVFVDDGCSRDVVLSRLSSEGCVSADASVVVERQDMSHAWSFERASDRSWCDPFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XV91</accession>
    <name>A0A8I6XV91_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XV91"/>
    <dbReference type="SMR" id="A0A8I6XV91"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0449710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0449710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0449710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0372470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0372470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0372470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0449710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0449710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0449710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="51"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0449710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="140" mass="15878" checksum="163CD3F2F5939FBA" modified="2022-05-25" version="1">MRAPRSRTGYVQEQVNIGSAPPGSKVKWFRSASDEPRFINTVTFDSKENAPTLVMVHAYGASQGFFFRNFDALANRFRVIAIDQLGWGGSSRPDFDCRSTEETEAWFIDSFEEWRKAKNFSNFILLGHSFGGYVATKYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXR5</accession>
    <name>A0A8I6XXR5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447916</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980573.1">
        <property type="nucleotide sequence ID" value="XM_045124638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXR5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0346790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0346790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0346790"/>
    </dbReference>
    <dbReference type="GeneID" id="123447916"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0289410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0289410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0289410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0346790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0346790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0346790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447916"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
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</entry>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
    <sequence length="158" mass="17571" checksum="376F39C803345E3F" modified="2022-05-25" version="1">MFLPSRVGFSPATCRMIMAPVLLDDSRAWCLYAFDMHAKILNILDPVHTLQDREVMAGLHAANADLLLAGLGEVGLALVEGFTINRDEWDIEYNIGINNPCSINESGVYLFHYARNFTGANLAEQIDEDGLVVLRKKLFYDIMAMDGNAGETPGFMER</sequence>
</entry>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="6">subsp. vulgare</strain>
        </source>
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        <property type="entry name" value="Rpb4-like"/>
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        <location>
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        </location>
    </feature>
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        <location>
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    </evidence>
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</entry>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
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    <reference evidence="7" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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        <subcellularLocation>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="3">
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0428090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24696" checksum="9B37AD79C9858D88" modified="2022-05-25" version="1">MPHLYDLYGMAHIASYKKAKAFSESDLDDPNNFTNISSHQKLVAYTDAGKATKGDDFNPSQDPLDPELVMISGGGRPHGSLAIGDGIIRCPLTLPEIKARQSSSCPEITRRPRPVELAIEAALQKERAANQAALEKERLASQAALQAALDERDQTTARLVEEERARNEAGQRALYELFVGLCEKSGQVPPPMPVFSSIGTNNSRAASHDPSPGQPSPNEAAASNRGVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y818</accession>
    <name>A0A8I6Y818_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123407702</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM161 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956826.1">
        <property type="nucleotide sequence ID" value="XM_045100891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y818"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643230"/>
    </dbReference>
    <dbReference type="GeneID" id="123407702"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0534490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0534490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0534490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0643230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407702"/>
    <dbReference type="OrthoDB" id="784140at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019395">
        <property type="entry name" value="Transmembrane_161A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13624:SF6">
        <property type="entry name" value="EMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13624">
        <property type="entry name" value="RE42071P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643230.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="40023" checksum="1B02EC8C43A08817" modified="2022-05-25" version="1">MAVDDLLPFPVPPYAPQVAASLALAAAAHFLHVPSLLLYAVHTYIHPDAVPSTGPRAVLRPPGSAAPSGKRGGGAAPASDGSAQLYRLRLSHATLASRPHFAGFHLSLLLPLALLPPALLLPPPRSPAAPLAPLLPLAFLFVALLRQVALASPPRPAHLVASLAALLAATVLSSSPFAGSVASLAALPAWRFARAFWLGTDQPRTGLAVLASSAPARLLLHLAVLVSSAASILQCCGFVDGPELEVKLLAAAAGLQLLASRAAVQMYLNEAVFCWYQRLHANRAPDTEYGRAKVFLHNHHLCAAATQFVAPPLLVLSLLALWWVQGKDFFEGVEGLDWLVGWSVAMKEAALLAARWVVAVWSAVTVGALVGYKRGWLFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y961</accession>
    <name>A0A8I6Y961_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COQ3</name>
        <name evidence="7" type="synonym">LOC123407486</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y961"/>
    <dbReference type="SMR" id="A0A8I6Y961"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0652110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0652110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0652110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0541240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0541240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0541240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0652110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0652110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0652110"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3265906at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
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        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
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        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
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        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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    </feature>
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            <position position="206"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
    </feature>
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        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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            <position position="211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0652110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="327" mass="35082" checksum="C33715A092A92AFC" modified="2022-05-25" version="1">MLRRLAPSLRRAAASSAVQRGGGGARLPDPGLSSPHALPPQWRRCASAAASSLPPPPPTPPQGPPRHAGGGSAASSLNPAEVAKFAAIAETWWDSQGPFKPLHLMNPTRLSFIRSTLCRHFRRDPYSAKPLEGLKVIDVGCGGGILSEPLARMGATVTGIDAVEKNIKIAHIHAASDPTTASIEYCCTTAEDLVKENKQFDAVISLEVIEHVANPLEFCESLSALTVPNGATVISTINRSMRAYAAAIVGAEYILRWLPIGTHEWSKLVTPEELVLALQRASISVEEMAGFVYNPLTREWSLSDDTSINYIAYGIKKSETPPTMPHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9Z6</accession>
    <name>A0A8I6Y9Z6_HORVV</name>
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        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123406982</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the splicing factor SR family.</text>
    </comment>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659950.1">
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659950.2">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
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    </dbReference>
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        <property type="entry name" value="Serine/arginine-rich splicing factor 7"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
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    </dbReference>
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        <property type="entry name" value="SRSF"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="110"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="217"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659950.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="290" mass="32647" checksum="59D5DBFE4DE401AC" modified="2022-05-25" version="1">MRGNFSKMENAVRGKPSVARKRPWFREGIRRTPAQRPNPNSKYIIPFVTPTSPPDPRAPPCFSPVPTSTRRVPVRRTPSRPHPIAAPAAVRRRRVPAGRPEKVGWSAAMARVYVGSLDPAVTARELEDEFRVFGVLRSVWVARKPPGFAFVDFDDRRDAQDAIKDLDGKNGWRVELSRNASSGRGGRDRSGGSEMKCYECGESGHFARECRLRIGSGGLGSGRRRSRSRSHSRSRSPRYRRSPSYSRRSYSRSPRRRSVSPARGRSVSRSPVRGRDESPAYSNGYRRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YB99</accession>
    <name>A0A8I6YB99_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YB99"/>
    <dbReference type="SMR" id="A0A8I6YB99"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23274">
        <property type="entry name" value="DNA HELICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23274:SF51">
        <property type="entry name" value="OS03G0423850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13746" checksum="398E0574A1B2693D" modified="2022-05-25" version="1">MIRAFQDNAIDAKIVGGQHAGKRVFIPRIPMSPSEDISLPFKLKRKQFPIRLSFAMTINKAQGQTIPNVGIYLPEPVFSHGQLYVALSRGCRGRRHGFWPSQTRRLINLGKEPRTLSIEMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDQ2</accession>
    <name>A0A8I6YDQ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDQ2"/>
    <dbReference type="SMR" id="A0A8I6YDQ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0670130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0670130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0670130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0556120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0556120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0556120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0670130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0670130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0670130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="6"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0670130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="259" mass="29093" checksum="FF438E126968DD0F" modified="2022-05-25" version="1">MADLEGFEFFTIILEKSSSRHVLPGNFMKMLDGRRPQNIKLRQADNGLCRLWDMEVLFETDGRMYLDRGWKQLVRAYDLRHGYFIVFRYDDNAMFTVKVFNTTMYRRRYQDHEDASNGSSRSNSGYSKSNSDSGYSKSSSNNGYSKRSSDYGYNKSSSEDEVEQSGEEEEQSRDAEVVVDDGLAMAVADNGLAMVVAEDDLAIVVPEDGHAMVVPEDGNVMVVADDDLAMVVVQDDGLAMVIPQLGDTTMPIVVEEYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YE12</accession>
    <name>A0A8I6YE12_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YE12"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0674090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0674090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0674090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0674090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0674090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0674090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0674090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="29060" checksum="DBFAEDDDD923C26F" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYTEYTVEEQYRDFNQSQYICTVRVFPDYPRAEKPIHWSYGLGVTVDMAVQDAAYSMLTIRRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YF66</accession>
    <name>A0A8I6YF66_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123397675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044948143.1">
        <property type="nucleotide sequence ID" value="XM_045092208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YF66"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462190"/>
    </dbReference>
    <dbReference type="GeneID" id="123397675"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0382600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0382600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0382600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0462190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397675"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF19">
        <property type="entry name" value="FACILITATOR SUPERFAMILY ANTIPORTER, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="39"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="476" mass="52139" checksum="A354FAB366E7C46F" modified="2022-05-25" version="1">MGESPSPAPAAVAKVYYDGCPGCAMERRKESTKGTPYKELFFVGIITFASSLPITSLFPFLYFMIQDLHVAQREEDIGFYAGFLGASYMVGRGFASIFWGMVADRIGRKPVIVFSLFTVVVFNTLFGLSVKYWMAITTRLLLGALNGFLAPIKAYSIEVCRDDEQALGISIVNTAWGVGLIIGPAIGGYLAQPAKQYPHIFHDKSTFGRFPYLLPCLCISIFATLALISCIWLPETLHKHTKIEMRAETAETGTSQESTESPKKSLWKNWPLMSSIITYCVFSLHDTAYSEIFSLWTVSGRKYGGLSFSSKDVGQVLTVAGASLLVYQIFVYRWLNNTLGPVNSTRIASALSIPIIAAYPFMTHLSGIRLGLALYIAAMIKSVLAITRVSGTSLLQNNAVPQGQRGAANGIATTLMSLFKSVAPAGAGILFSWAQKRQHAAFFPGDQMVFLLLNLTEVLGLLLTFKPFLAVPEHYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YG92</accession>
    <name>A0A8I6YG92_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123401375</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044951097.1">
        <property type="nucleotide sequence ID" value="XM_045095162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YG92"/>
    <dbReference type="SMR" id="A0A8I6YG92"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545380"/>
    </dbReference>
    <dbReference type="GeneID" id="123401375"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0453410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0453410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0453410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0545380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401375"/>
    <dbReference type="OrthoDB" id="649067at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF412">
        <property type="entry name" value="OS04G0307900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="585"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="897"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="909"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="925" mass="103007" checksum="9DA68A31BE8FA5F0" modified="2022-05-25" version="1">MGIAGVLLVLFLLLVLCPLLPVLIMVRASGLKGTLPSAAMLADCPKRCGNLTFDYPFGIGTGCFRNPDFKLTCDDTAQPPKLFLCDGTTQVFQNIVTIDNFSPVLYSFLNLRIPINISHKIDMRSGVDVYHMFWKSPGRSFSLSYAQLNITGCGFHAYLVGNETGSALDDCEASCPVEGITDMVARKICNGTGCCSINFSDYGFAPRTFHLKFVRHEHGKLKPRNSDSSLRDRIHITSSTSLQWSIVDQANCHYTEKNKTNYACISKDSRCLSADGFGYYCYCDDGYGGNPYILEGCSRDTTGYIPAQQKDDCTRSCGNVSVPYTFGLAEDCFGREIFYLNCTNSTSPVLLLDQYYHVTNINVNEGLIEYVDPGTGWDETVLTILQGQRLYYTGSNQTSSRQWAVANLTCLEAQQISRYACVSSQSTCVPVTSYRGYFGYRCKCKDGFHGNPYIKQGCEDIDECLAPNNCTGICLNTVGTFTCTDCPPKTEYDPTKRQCTPTKQQNLLIGIIIGLCSGVGILLLGLFVIFLVVTWKRNIQRQQRRAHFRKNKGLLLEQLIASDENASDKAKIFSFEDLKKATNNFDPTRILGRGGHGTVYKGILANKHVVAIKKAMVIEEDEINQFINEVVILSQIDHRNIVKLFGCCLEAEVPLLVYDFIPNGSLFGLLHGNSSSELALHWDDCLRIAAETAGALCYLHSSASVSVFHRDVKSSNILLDGKCTAKLSDFGASRLVPIDKTHVVTNVQGTHGYLDPEYFYTAQLNQKSDVYSFGVVLLELLLRREPIFTVHSGFKQNLSNYFLTEINTRPVTEIVAAQVLEEATEEEMENIALLAEMCLRPRGEERPTMKQVETKLQILQAKRLSARPVTRDNDAERKPLLNARTRANYQSLSVDCEDPASSSWQSSQNCYSLEQESSTSTTRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGI3</accession>
    <name>A0A8I6YGI3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
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                <person name="Muehlbauer G.J."/>
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            <authorList>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF11">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN RICESLEEPER 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="11">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="800" mass="91638" checksum="FF4E7AD994B806B9" modified="2022-05-25" version="1">MENMDEYDSDANMPLGIDSDGAAASRFRKKRSKVWEEYKPIYINGVIQSAECHYCHTLMSCKGADGRSNGTSHLWRHHNNCRAKEEFDLSELHDTNFPYGVNDIDPVNQILPEPLDDMNMVNHSENGRFRSKVWMDFTPVYVEGRIQGADCVHCHTRLSAEKVTGDLNQHTQTCPAQAGTSVNHQKGGLFPSNVPIFKSRVKDELLPALTNGRVQIAEYASRFHLGYNSGDKTCQNQHILALPADNMTPMEQDKSSARTPDIKIRKFDQEACYQELTRMIIMHGYPLSIVEHEEMKRFAKGLNPVFNMASSLDMEEYSTLLFQKEKSDLKEKIALSSRRVSLSASVWVPHGAEPTIKYLCLTAHFIDAEWKLQRRIIKFGVLWSSPSNLERMIHCKEACVLESEVGAYNVIWEAIREWNLDRKLFCMTSVSEIRNSGSISKLKDMLIQRNCLPIRGELYNIACVDDMLDNILSKGQSLLYRVGDLLERFIQALASSLLTQQQLLEVAKDVGMKCPHEDAKWWHKIYFRLEVLLHSKKVLPSEEFVSVEEMKIVEPFCKILRVFYRVVEVMSGPVCLTANMHFHEVWKVRTILEEEACTEHSDVASMIREMQEAFNEYWAKSYLWLSVPVVLDPRFKITFIDFRLKRAFGADATKYVNDLAEIVRELFHEYCTPMDQPNVQTANCGVHNVEIDGFDSDSLEDWDHHLTAQTSSQLLSELDSYLEDGLLPRKDDFDILNWWMSHSAKYPTLSTMAQDVLAMPSSSVHCQAAFSSEGPVIHKEWSTLNIKTIEALVCTRDWIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJT9</accession>
    <name>A0A8I6YJT9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123411988</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960882.1">
        <property type="nucleotide sequence ID" value="XM_045104947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJT9"/>
    <dbReference type="SMR" id="A0A8I6YJT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659520"/>
    </dbReference>
    <dbReference type="GeneID" id="123411988"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0547300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0547300.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0547300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0659520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0659520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0659520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411988"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000364">
        <property type="entry name" value="Pentatricopeptide repeat (PPR-like) superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000463">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF452">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="381"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="451"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="482"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="553"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="698"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0659520.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="790" mass="85974" checksum="A43465A96D4F305B" modified="2022-05-25" version="1">MPSPRLPPTAAALFADVGALRRSYVRLIALSSTLRHLDQLLAVSLASGHYTLDPAPATSLLLRYASLRAPPRQLLRLFRAFPNPDRFIRNALLRSLPSLRPDLLFPSPDSFSFAFAATSLNASSSRRGIVSPSASARALHGLAVAAGYAGDTFVASALAKLYSTLSRADDARKVFDAVPSPDTVLWNTLLAVLSGSEAMEAFVRMVREGSAQPDSTTLSSVLPAAAEVADVTMGRCVHAFGEKCGLAQHEHVVTALISLYAKCGDMECARRLFDRMVAPDLVAYNALISGYSVNGMVGSSVELFKDLVTLGLRPTSSTLVALIPVHSPFGHEQLTRCLHAHVVKAGFDANAPVSTALTTLYCRLNDMDSARKAFDAMPEKTMESWNAMISGYAQNGLTEKAVALFQKMQALNVPPNPLTISSTLSACAQLGALSLGKWVHKIIAKENLELNVYVMTALIDMYVKCGNIAEARRIFDGMDNKNVVSWNAMISGYGLHGQGAEALKLYKYMLDANLLPTSSTFLSVLYACSHGGLVEEGRTTFRSMTSDYGLIPGIEHCTCMVDLLGRAGRLKEAYELISEFPKSAIGPGVWGALLGACMVHKDADLAKLASQKLFELEPENTGYYVLLSNLYTSKKQYSEAAGVRQEAKSRKLVKTPGCTLIELGDKPHVFMSGDRAHPQSHAIYSYLEKLTTKMIEAGYQPDTEAALYDVEEEEKENMVKVHSEKLAIAFGLLNTEPGTEIRIIKNLRVCLDCHNATKIISKVTQRLIVVRDASRFHHFRDGVCSCGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YML3</accession>
    <name>A0A8I6YML3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123399001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044949372.1">
        <property type="nucleotide sequence ID" value="XM_045093437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YML3"/>
    <dbReference type="SMR" id="A0A8I6YML3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521610"/>
    </dbReference>
    <dbReference type="GeneID" id="123399001"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0433540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0433540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0433540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0521610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0521610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0521610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399001"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000333">
        <property type="entry name" value="Serine/threonine-protein kinase AGC1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000295">
        <property type="entry name" value="Serine/threonine-protein kinase AGC1-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000028">
        <property type="entry name" value="serine/threonine-protein kinase D6PK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="118"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="475"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0521610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="512" mass="55192" checksum="02B43F3B9BF5EE3D" modified="2022-05-25" version="1">MERIRFRSVDAGAGTYPTTLPSPRMLDAGATEPPGFRKSMNPIYADGTARSASTAAISASLDAPGLDGSRRSHADGDVESGSNSVAGVLVRRHTGGDGRWEAIRAADARESPLSLGHFRLLKRLGYGDIGSVYLVELRGAAGGGALFAMKVMDKGSLVSRNKLSRAQTEREILGLLDHPFLPTLYSHFETDKFFCLLMEFCSGGNLHSLRQRQPSKHFTEHAARFYASEVLLALEYLHMLGVVYRDLKPENVLVREEGHIMLSDFDLSLRCAVSPTLVRTPSGRVGAGGLVHGCKLPRILSSSKAKGKKKLAAAKASQQELVPGDGKKQPWTSLEFMAEPTGARSMSFVGTHEYLAPEIIRGDGHGSAVDWWTFGVFLYELLHGTTPFKGSGNRATLFNVVGQPLRFPDAPGVSAAARDLIRGLLAKEPQKRLAYRRGAAEIKQHPFFEGVNWALVRSAAPPYIPDAVDCGPVPVRLPSDAPSQGGTPRSAAAGKAGSPHADPASYVDFEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YP98</accession>
    <name>A0A8I6YP98_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123411759</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960660.1">
        <property type="nucleotide sequence ID" value="XM_045104725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YP98"/>
    <dbReference type="SMR" id="A0A8I6YP98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712440"/>
    </dbReference>
    <dbReference type="GeneID" id="123411759"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0590950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0590950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0590950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411759"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000011">
        <property type="entry name" value="B-cell receptor-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF18">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="226" mass="24633" checksum="B345B6C475FBA10D" modified="2022-05-25" version="1">MIQLLFTVLAAEAGLAAVLLFKTPLRKLAMLALDRLKRGRAPVMVRTVAATVLVVLASSLHSMAKIHGHAGAGELDAPGALSPTDQVLLARHLLEASLMGYILFLALVIDRLHSYIREMRGLKKNLEAVSKQNKTLEEARSGKSDESKPHHNDMASLNEEIKKLKLQLKEKAQEAKDAEAKAVAAQTQSEGLARKYDSLLEDNKHLHEQLQSGGIPLSRSDGKKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPJ6</accession>
    <name>A0A8I6YPJ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPJ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0623010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0623010.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0623010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0751100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0751100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0751100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF90">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="89"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48237" checksum="117EEDF33BC1447B" modified="2022-05-25" version="1">MGLSLGKTATPGNGAAEEHGVVGSPWPDLPPELAAEILARLLSHTDRLSFGVVCRGWRLAAREQGLLLPPSMPWINIGRGAYMDLAGNGKACRFATPKGYRARATFGSWVLYEHDLSSRCFLGDPFSPSTPAIEVPCHYKFNAIICHNSVSMRFCQNGSFLTWPLKKMVVCSPHLIVATSAYNCPLKIACFRPSNGMAIKPATLLWSDGDYLHGHQHHACDFTYKDIAFQHGKIFALSLTENLFAYDFATEEMWLSQTWSRVEHAIKERPVAAAGAKLDYHNHHLLTSSDKQKLLMVRWRIPLQEDRTKFDHRPMDLQVFEADLQKGRWSQVKDLGGQALFIGQTGSRALAVGDFSDGMFQANRVFILGDDWALYHASRPWCKCFDCKKLGYKVPSYCVYDMISGEASLVSLGGRDHCLIKSPKMAWFFPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YUM7</accession>
    <name>A0A8I6YUM7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Signal peptidase complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Microsomal signal peptidase 22 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123451935</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPCS3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984434.1">
        <property type="nucleotide sequence ID" value="XM_045128499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YUM7"/>
    <dbReference type="SMR" id="A0A8I6YUM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504710"/>
    </dbReference>
    <dbReference type="GeneID" id="123451935"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0419190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0419190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0419190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0504710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451935"/>
    <dbReference type="OrthoDB" id="10261524at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035218697" description="Signal peptidase complex subunit 3" evidence="10">
        <location>
            <begin position="26"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="18839" checksum="8BBCBF4F19C625CE" modified="2022-05-25" version="1">MHSFGHRANAVATFALTILAAMCLAASFSDSFNSPTPTASVKILNINWFQKEANANDEVSMTLNISADLSSLFTWNTKQVFVFVAAEYETPQNALNQVSLWDGIIPAKEHAKFLIHTTNKYRFIDQGSNLKAKDFNLTMHWHIMPKTGKMFADKIVMTGYQLPEQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVT8</accession>
    <name>A0A8I6YVT8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVT8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0619550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0619550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0619550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0619550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0619550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0619550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF25">
        <property type="entry name" value="TTF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0619550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="381" mass="43585" checksum="712E3611B0A22E45" modified="2022-05-25" version="1">MQPDLNKFPRTEFGSSRTSRAFSKSWYKKHPWIEYSEWKKAAYCFYCFLFKQPGRAEHFGYEVFTKEGFTDWKHASKAFKDHIGGPGSKHNSCVKHYDDYSNQRQSVASNLARASKESEEMYKIRLTCSVSCSRYLIAQGLAFRGHDESSTSLNKGNFREMIDLEKTKNEQVRDAFDRGARNCTMTSGDIQKELAMCCAHEVTKRIMAELGDKQFSVLIDESRDISVKEQMAVMLRFLNDKGKVVERFIALYHVKDTTSEALKNALYGILDHYKLSISRIRGQGYDGASNMRGEFNGLQKKIIDENPFSLYVNCYAHRLQLVVVSVASSCSSIHDFFEYISLIVTTTTTSCKRRDALAEAQHQDILDRLESGEICIGRGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YZW9</accession>
    <name>A0A8I6YZW9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123409772</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958574.1">
        <property type="nucleotide sequence ID" value="XM_045102639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YZW9"/>
    <dbReference type="SMR" id="A0A8I6YZW9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655490"/>
    </dbReference>
    <dbReference type="GeneID" id="123409772"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0543920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0543920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0543920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0655490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409772"/>
    <dbReference type="OMA" id="TNEYEHN"/>
    <dbReference type="OrthoDB" id="1898734at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="110"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="505" mass="56244" checksum="7976DEA2741316FB" modified="2022-05-25" version="1">MAVVTSSLRCATAAALALALHHHHQPAFLDVRLRFPRHRIPTRMAMSSSPASAPHQAGPWYAVPDLSLRDHSFAVPLDHSSPASPSAPHITVFVREVVTAGKEEAPLPYLVYLQGGPGYESPRPTEASGWVKKACEEYRVLLLDQRGTGLSTPLTTSSLSQITSAAEQVEYLKHFRADNIVKDAELIRRHIVPDAKPWTILGQSYGGFCAVTYLSFAPEGLKAALLTGGLPPLGKPCTAETVYRACFKQVQQQNEKYYKRFPQDIQVVHEVVRYLSESEGGGVLLPSGGRLTPKMLQCLGLWGLGFTGGFERLHYLLERVWDPILVPGAKKSISYYFLKEFDMWVGFDQNPLYALLHESIYCEGSSSKWTADKIFNENRSLFDPVKATEEGRPVYLTGEMVFPCFFDEINALRPLKEAAHLLAEKEDWPPLYDISALNNNKVPVAAAVYYEDMYVNFNIAKETASQIAGIRLWVTNEYPHSGLRDGGPHVFEHLMGLLKGKKPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0C8</accession>
    <name>A0A8I7B0C8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123443044</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975248.1">
        <property type="nucleotide sequence ID" value="XM_045119313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0C8"/>
    <dbReference type="SMR" id="A0A8I7B0C8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005270"/>
    </dbReference>
    <dbReference type="GeneID" id="123443044"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443044"/>
    <dbReference type="OrthoDB" id="604685at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010208">
        <property type="term" value="P:pollen wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018088">
        <property type="entry name" value="Chalcone/stilbene_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF73">
        <property type="entry name" value="CHALCONE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00441">
        <property type="entry name" value="CHALCONE_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="241"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="43218" checksum="4A801E533CB79313" modified="2022-05-25" version="1">MAAAVKLEEVRRAQRAVGPATVLAIGTAVPANCVYQATYPDYYFRVTKSEHLSDLKEKFERMCEKSTIRKRHMHLTEDILKKHPSICSHMEPSLNTRHDIVVVEVPKLGKEAAERAIKEWGQPLSKITHVIFCTTSGVDMPGADYQLTRLLGLSPTVKRLMMYQQGCFGGATVLRMAKDIAENNRGARVLVVCSDITAMAFRGPSKSHLDSLVGHALFGDGAAAAIIGADLDEPFEKPLFQLVSASQTILPESEGAINGHLIEAGLTIHLLKDVPGLISQNIEQALEDAFKPLGIHDWNSIFWIAHPGGPAILDMVEEKVGLDKERMRASREVLSEYGNMSSACVLFVLDVMRKTSSQDGHTTTGEGKEWGVLFGFGPGLTVETLVLYSAPIIATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B0G1</accession>
    <name>A0A8I7B0G1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B0G1"/>
    <dbReference type="SMR" id="A0A8I7B0G1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0010750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF236">
        <property type="entry name" value="NEPROSIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="1">
        <location>
            <begin position="82"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="336" mass="36724" checksum="7E4F72193849527E" modified="2022-05-25" version="1">MPFQYSRRFSALYKEASPLQNCRRQTTRSGPLHLEMKKSTSIQQVVSFAVLLANAIVGRCSLEVWPPFEGHLEYLQPGVALASSRNSLSSYVAHKAWIPGDDNNYYYGVEATLDVYGFNLQPGQLTEGGVWIASTGDGHPIPDNGIQIGWHIYPGMHKDSRTHFYVGWGAKGSPDKGCFNMVCPGFRKTSSSIAPGDVIDPVSSINGTKQYITVRVFKDKSSGDWHVHYGLNGPPKPVGYFPKSLLPAMIDRPVLLRFGGYAARKKPAPNPPMGNGYVPLSSTAALVSNLKLIDADGNDHIVNTDLPYFVTSKQCYPLSVVDSGQFFYGGPGCVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1W0</accession>
    <name>A0A8I7B1W0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chalcone/stilbene synthase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1W0"/>
    <dbReference type="SMR" id="A0A8I7B1W0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0098180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0098180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0098180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0098180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0098180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0098180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF24">
        <property type="entry name" value="OS07G0526400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0098180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="17049" checksum="498D08018CC503C1" modified="2022-05-25" version="1">MVSASQNTIPATDGVLTMQLTEAGLDGHIFTRELIPLAAKHIEQCLTDAFEQLGIICGGTKWNDLFFVVHPGIRGIMDHIQRALRLDPGKLAASRTVLRDYGNMLGATLIFVLDEQRRQMEETGEWGVMMGFGPGFTVETMLLHSVARNLHDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1Y0</accession>
    <name>A0A8I7B1Y0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953582.1">
        <property type="nucleotide sequence ID" value="XM_045097647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1Y0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001980"/>
    </dbReference>
    <dbReference type="GeneID" id="123403730"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0002520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0002520.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0002520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403730"/>
    <dbReference type="OrthoDB" id="680497at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF247)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549:SF226">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001980.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="520" mass="59465" checksum="AAE2F5BDB89D09E7" modified="2022-05-25" version="1">MSMEPWSWVVTCDPGCWSMEETAAACSSDNRISFQTSLPSHSDSGMQLQVVEYAATATATATHDICSETVQVFEEVTQVFNDNYLVIMKDKMHKFPPSLGDVPDWWKVPATVAIGPYYHGEPGLLEAEQVKHSAANQCIRDSPVASLQEMYNAVFAVLPDALNLYYQDELAKFRHDDLLPMMFIDACFLVQFMRWYYLPEGDMHQALESYFSANYERICTDIMMLENQIPWVVVKTIFNLMPPAPSPWLSPWERFVIVMRRGLGNQVGPEDNIDVDPEYHPPHVLGLIWHYIVRNNNHHGDDDDEEDDHNNEDDDNDNDKDDDDKQKPLSISVAELAAIGITLVPTRKEWKAGLMSMRLKKKPRCFFVFGDLEVRPLYLSEGNAAWLVNMAAFELCKTPDFQSDHVHDRDSAVCSYLHLFAMLLDQQQDVHELQKEHLIEGGGLTSKMTLHFFASIGKNMRPGTFYLRIIMRIEKFRNDRSRLLRCILFLKRKRAKVMALISIIAAVVGILSSLQALKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B207</accession>
    <name>A0A8I7B207_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123429243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044969233.1">
        <property type="nucleotide sequence ID" value="XM_045113298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B207"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0103750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0103750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0103750"/>
    </dbReference>
    <dbReference type="GeneID" id="123429243"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0103750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0103750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0103750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARID" evidence="2">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0103750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="292" mass="31339" checksum="F8D5D7B9DE442236" modified="2022-05-25" version="1">MATPPSPPPSMNSSACPFKTNIPSPKPRGAPAVGASPVSSGPRHIVVYVRDIVNKLRAGGHLSGLDTPDAELTEEGTSALFEGVLEAFLAEVRASAGLPLLPKPLMIGKDGPVEMQMLRLHLAVRSSGGYAAVTSWAAIAEVVGLEPVAAAPLKLLYFKYLLPLEQSIGNCKPEMQDQEVAGGCRDAVAARRSNGKKDQASLRSSGHLKRKRGAPVPLQMLNWVRLVAKSPLEHGNGICSQFSSTLMLRRLRFANVDCSQLAQSTTSQQSHGFKTLPKKSSTLRRLRRRCHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B255</accession>
    <name>A0A8I7B255_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123424650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044964229.1">
        <property type="nucleotide sequence ID" value="XM_045108294.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964231.1">
        <property type="nucleotide sequence ID" value="XM_045108296.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964232.1">
        <property type="nucleotide sequence ID" value="XM_045108297.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964233.1">
        <property type="nucleotide sequence ID" value="XM_045108298.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964234.1">
        <property type="nucleotide sequence ID" value="XM_045108299.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964235.1">
        <property type="nucleotide sequence ID" value="XM_045108300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B255"/>
    <dbReference type="SMR" id="A0A8I7B255"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110110"/>
    </dbReference>
    <dbReference type="GeneID" id="123424650"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0090370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0090370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0090370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424650"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818">
        <property type="entry name" value="RNI-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035208262" evidence="1">
        <location>
            <begin position="24"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="646" mass="70927" checksum="1A63C7857B6E282E" modified="2022-05-25" version="1">MAAAGAKAVPPTLLSLCLQAVAAHLTADAAGSGRSGGCGAHFDGLVQELEEEEEEGGGHLAPEQVAEALPWELLHQLASYLPPFALELLHHGAHVRCCSSASLTDRFGEAGGNRRGTKRSRCEDFNTAWQALFEFRWPLHDNTGHDDLVTLDWQQKYWEKHLQECLDEAAEAASLPSFRGHIGELSMSDKTMSCVYLSENIAQQYSRLSYHWTILGSYARCLRFQNVLCTAEICDMLQHSRLETLVFVRIISELEVNAVCRLLSCHARTLLSLEFIHCQLYPAVMDKVCTSLYQPGSQNYKIQSFSIKSSRISESKSLTTSAGLLNFLSCAKSLQLLSFNDAKMQPSFAKIIIRTLLEASSGLHTLDMSENNFAGWFSTADRSSTSLSSALKSNTSLNSLTVLNLRGNNLQEGDMEDVCKILAKMSNLRNLDISDNPITDVGIRYLMPFLELALQRGNPLQRLRAENCDLSSIGVTKLLECLTSINKPLDMLSIADNHLGSSVAATLVKFLGSHVRELNVEDIGLGPIGFQILEEALPRKVNLSHINISKNRGGMRTAYFISRLILQAPNLVSVDAGSNLLPPESLEVICNALKQTTCNLARLDLMGNLQLSSAIFPAVFEFNKHGKPILLVPSEQGSCAPYDADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B310</accession>
    <name>A0A8I7B310_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B310"/>
    <dbReference type="SMR" id="A0A8I7B310"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0162800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0162800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0162800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0162800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0162800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0162800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47169">
        <property type="entry name" value="OS01G0541250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47169:SF2">
        <property type="entry name" value="OS01G0541250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0162800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="33811" checksum="7EEA0E7EEFFA80CC" modified="2022-05-25" version="1">MKDRMKFCLSMLDETTIETGRPKFNTMHNIVHIDEKWFYMTKNRNYYLLDEEEEPTRTIQSGASIGKVMFLTVVVQPKWDSEGNVTFSGKIGILPFVKEVPAQRKSDNRPRGTMETKSMKVNRKVMREFMIDNLLSAIQSAWPENDVGKTIFIQQDNAKPHILPNDPKNLAAVESIVLDIRLIQQPANSLDLNGLDLGFFSSLQSLTDCVSPRTFQDLIQGVLEEFENYEVYKLNRVFLSLQACMIENLNHAGGNGYKIPHVNKERLESLGVLPLRLACPQEVYANAIHNLQLMEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4C7</accession>
    <name>A0A8I7B4C7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123428504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044968658.1">
        <property type="nucleotide sequence ID" value="XM_045112723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4C7"/>
    <dbReference type="SMR" id="A0A8I7B4C7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0214910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214910"/>
    </dbReference>
    <dbReference type="GeneID" id="123428504"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0178650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0178650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0178650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0214910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428504"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45376">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45376:SF7">
        <property type="entry name" value="OS04G0687300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
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        </location>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="271" mass="30868" checksum="A026CD01D4502291" modified="2022-05-25" version="1">MQAAAPWSKGLPGLRSHLCAAASFHSTPASPAKWKGKFDSKHEHGERKLSKKYERYVVRQKRAEGKKDLKNYLLYGKSTPHIQDGSTGSFANSHDIPRFKTFRKRQDHSSTKPRQGVHHQRKDKKDKAKFYNFFYEAHYVNPEKVFETIFGEHHQGFTWSHASWEGFHFRDSSFGFRRAGESRRQRVESEDESDDDDTCEAAGVGSHAHRVTLGLPPRGPLTLDDVKTAFRASALRWHPDKHPGSSQAVAEEKFKLCVNAYNSLCTVLKTA</sequence>
</entry>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15226" checksum="8D96CEA7E3727E7F" modified="2022-05-25" version="1">MLKRRHYHIVDMESCVLCTTQEEETIDHLIFNFPFSQACWAKNGISYNSNISRIGATIRAKEIYSGRLFFEIFTITAWNIWKERNNFIFNQISPTYNAWLQGTKADLSWLRYRVSTNLSDYITSLVNNL</sequence>
</entry>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="10" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="TMEM85/Emc4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06417">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <begin position="77"/>
            <end position="102"/>
        </location>
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        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="177" mass="19257" checksum="8018D10210815941" modified="2022-05-25" version="1">MEKGKGLARRWAVELHDVSSSSSSPAFPDPPGFTRSAPEADDAASARQRKETEAAWKGQKAWEVAQAPFKNLMMMGFMMWMAGSTVHLFSIGIVFSALWQPFNALRSVGKVFEPFKDPRVDTLAPKLLFTALNLAAMGLGVWKLNTLGLLPTNASDWVSSLSPAREVEYAGGGIPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B790</accession>
    <name>A0A8I7B790_HORVV</name>
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            <taxon>Triticodae</taxon>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.</text>
            <dbReference type="EC" id="3.2.1.55"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 51 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.55" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044981714.1">
        <property type="nucleotide sequence ID" value="XM_045125779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B790"/>
    <dbReference type="SMR" id="A0A8I7B790"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380530"/>
    </dbReference>
    <dbReference type="GeneID" id="123448777"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0380530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448777"/>
    <dbReference type="OrthoDB" id="406864at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046373">
        <property type="term" value="P:L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000011">
        <property type="entry name" value="Alpha-L-arabinofuranosidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000025">
        <property type="entry name" value="Alpha-L-arabinofuranosidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000063">
        <property type="entry name" value="Putative alpha-L-arabinofuranosidase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010720">
        <property type="entry name" value="Alpha-L-AF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055235">
        <property type="entry name" value="ASD1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051563">
        <property type="entry name" value="Glycosyl_Hydrolase_51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31776">
        <property type="entry name" value="ALPHA-L-ARABINOFURANOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31776:SF0">
        <property type="entry name" value="ALPHA-L-ARABINOFURANOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06964">
        <property type="entry name" value="Alpha-L-AF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22848">
        <property type="entry name" value="ASD1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00813">
        <property type="entry name" value="Alpha-L-AF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-arabinofuranosidase C-terminal" evidence="12">
        <location>
            <begin position="494"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="709" mass="78367" checksum="6D05F325C1E6A15D" modified="2022-05-25" version="1">MRGRRPRNAALRPRFLPQIRHWRASAIPEKVAMAVLRGSLNGWLFWVLGLVCAMYQIFFVGSAAGQSAQLSVDASHQNTQMIPENMFGIFFEEINHAGAGGLWAELVNNRGFEAGGPNTPSNIDPWLIIGDESNIIVATDRSSCFATNPIALRMEVLCQSSGNDVCPPGGVGIYNPGFWGMNIEEAKVYKVSMYIRSSDSMDLTVSLTSSDGLQNLAAYTITADKEEFKEWTKVEFDLQSSGRNYNSRLQLTTTKSGIVWFDQVSLMPSDTYMGHGYRKDLASMLANLKPKFLKFPGGNYVMGNYLLNAFRWSETVGPWEERPGHFNDVWGYWTDDGLGFFEFLQLAEDLGACPVWVVNDGASRYEQVSSATIAAFVKDVVDSIEFARGDPGTSWGSVRAAMGHPEPFQLNYISMGNQECSMHYYKENYRKFYSAIKASYPDIKIISSCDRSTISPVAPADLYDVHVYTSSGDMFSKSSMFDSTRRGGPKAIVSEYAVTGNDAGRGTLVAALAEAAFLIGLERNSDAVEMASCAPLFVNDNDRRWSPDAIVFNSWQHYGCPNYWMLHFFKDSSGAALHPSTIQVSNYNQLVASAITWQNSKDGNAYLKIKVLNFGNKAVDLSISITGLENGIQTFGSIKTVLTSGSLWDENSFQQPDKVVPVESPITNAREEMSLVLDPYALTSFDLLLDQSSSTMMHPVPESSLHSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAD3</accession>
    <name>A0A8I7BAD3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAD3"/>
    <dbReference type="SMR" id="A0A8I7BAD3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0528670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0528670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0528670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0528670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0528670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0528670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF15">
        <property type="entry name" value="THIOREDOXIN Y1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0528670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="74" mass="8464" checksum="221A296C996F199C" modified="2022-05-25" version="1">MVPILQEVHEKLSDKINIVKIDTEKYTSIANKYKIEALPTFIIFKDTEPCYRFEGALPADQLILQIESALEAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAW3</accession>
    <name>A0A8I7BAW3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123405195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NAR2.1. Imports nitrate with high affinity when expressed with NAR2.1 in a heterologous system (Xenopus oocytes).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044954915.1">
        <property type="nucleotide sequence ID" value="XM_045098980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAW3"/>
    <dbReference type="SMR" id="A0A8I7BAW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543390"/>
    </dbReference>
    <dbReference type="GeneID" id="123405195"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451920.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0543390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405195"/>
    <dbReference type="OrthoDB" id="605532at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071249">
        <property type="term" value="P:cellular response to nitrate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015706">
        <property type="term" value="P:nitrate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000048">
        <property type="entry name" value="High affinity nitrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000053">
        <property type="entry name" value="Nitrate transporter 2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="507" mass="54674" checksum="2B2219AFBAB22F9D" modified="2022-05-25" version="1">MEVEAGAHGDTAASKFTLPVDSEHKAKSFRLFSFANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLAKADIGNAGVASVSGSIFSRLAMGAICDLLGPRYGCAFLVMLSAPTVFCMAVIDDASGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIGTVNGLAAGWGNMGGGATQLIMPLVFHAIQKCGATPFVAWRIAYFVPGMMHIVMGLLVLTMGQDLPDGNLASLQKKGDMAKDKFSKVLWGAVTNYRTWIFVLLYGYCMGVELTTDNVIAEYYFDHFHLDLRAAGTIAACFGMANIVARPMGGYLSDLGARYFGMRARLWNIWILQTAGGAFCIWLGRASALPASVTAMVLFSICAQAACGAIFGVAPFVSRRSLGIISGLTGAGGNVGAGLTQLLFFTSSQYSTGRGLEYMGIMIMACTLPVALVHFPQWGSMFFPASADATEEEYYASEWSEEEKGKGLHIAGQKFAENSRSERGRRNVILATSATPPNNTPQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB02</accession>
    <name>A0A8I7BB02_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123445396</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978309.1">
        <property type="nucleotide sequence ID" value="XM_045122374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB02"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0315380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0315380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0315380"/>
    </dbReference>
    <dbReference type="GeneID" id="123445396"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0263460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0263460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0263460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0315380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0315380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0315380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445396"/>
    <dbReference type="OMA" id="PRLACRH"/>
    <dbReference type="OrthoDB" id="594350at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045009">
        <property type="entry name" value="CASPL-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32021">
        <property type="entry name" value="CASP-LIKE PROTEIN 5B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32021:SF51">
        <property type="entry name" value="CASP-LIKE PROTEIN 5B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="7"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0315380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="17388" checksum="6DAC761590A2A9E3" modified="2022-05-25" version="1">MKRVVGSPGTWSGMVLRLSQCVFASASTFAMVSGFGYSNYSAFFYLNVSLILQLMWSLFLACKDIFALRNKKDLHTADNLLFIVLIDFAVAIFMFSAACASASLTVFFMWDVHLCKAYPRLACRHFGLSVVLAFITWFLQAASSFSGFWLLVSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB96</accession>
    <name>A0A8I7BB96_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.47</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB96"/>
    <dbReference type="SMR" id="A0A8I7BB96"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0463840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0463840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0463840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0558390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000924">
        <property type="entry name" value="GDP-mannose 4,6 dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715:SF1">
        <property type="entry name" value="GDP-MANNOSE 4,6 DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="GDP-MANNOSE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="6">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="193" mass="22144" checksum="052203DAE77FFB59" modified="2022-05-25" version="1">MRYYQAGSSEMFGSTPPPQSEATPFHPRSPYAAAKVAAHWYTVNYREAYDICACNGVLFNHESPRRGENYVTRKITRAIGRIKAMWLMLQQDKPDDYVVATEECHTVEEFLQATFGYAGLNWKDHVVIDKKYFCPSEVDCLQGDSSKSRRLLGWKPKVGFQQLVEMMVDNDIELAKKEKVLVDAGYRDPKHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BBE3</accession>
    <name>A0A8I7BBE3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044952891.1">
        <property type="nucleotide sequence ID" value="XM_045096956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BBE3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0567350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0567350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0567350"/>
    </dbReference>
    <dbReference type="GeneID" id="123402983"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0470210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0470210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0470210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0567350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0567350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0567350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402983"/>
    <dbReference type="OrthoDB" id="635642at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055566">
        <property type="entry name" value="ARM_LIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056514">
        <property type="entry name" value="ARM_LIN_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056512">
        <property type="entry name" value="LIN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35549:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35549">
        <property type="entry name" value="OS04G0584500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23568">
        <property type="entry name" value="ARM_LIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23654">
        <property type="entry name" value="ARM_LIN_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23628">
        <property type="entry name" value="ARM_LIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN N-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN ARM repeats" evidence="3">
        <location>
            <begin position="414"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN ARM-like" evidence="2">
        <location>
            <begin position="579"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0567350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="945" mass="103639" checksum="60C2E29EBA241392" modified="2022-05-25" version="1">MPPPPPSSSSSLRDLLARECSELPEPAAAPCRTVSSRHGADDAVGTVVSMLSGYAGRFSKDAEFRRALREKCAPCLAPAGEAAAKEHAVLLADLELAIESIERLADASPSPRDSKIRSLRNSIRLLGVVAALHAPPGPADSGEGDARGVPSSHLSACAQLYLAVVYKMESDPHLAARHLLQVFVDAPHLARKTLLPDLWAHVFLPHLLHLEVWLANESELVAGCDGGDADGRSSRRMKTLQRLYDDQMDSGTAQFAMYYKEWLKHGGDAPPATPSVPLPSTPWSFDKWEKHSSSLRTSSINRNLYNAVFGISFEQEDAKLPDEAEYVLDMDVQLDENPGSLKMEKLAHRNIGLQEKQSSIRKESTIPETAPTPRKSYSLRLLSCRGDVSRNVINHPKVPKRNVSVEKELDCSEVAVTLQRAVSTVSNSDSLTQCEYAVHEIASACSNLGGGPNLGTWMSCPSFIQGLLEVTFTSKDDAVLESAILIMGELVLRNEVNRQIVLNADPQLEVFLRLLALRSNGLFLKATAVLYLMKPRAKQMLSMDWMPLVLHILECGDEVQLLSSLKCYPKIAAFYFLDQLLTGFDIDRNVENAKQMIALGGLDLLMSRLEVGDARESRICISLLTSCVQADGSCRYYLADNLKKEPLVQLLVGNQKKASAAALNLMSELTCLNRTCQTVEFLKELKNGGCLNTMQILLVYLQQAPPVQHPLAAVMLLQLDLLGDPLQYSVYRAEAIDAIMAALEHSSQSVKVQEQCARALLLLAGRFSSSGEPIAEAWLLKRAGVDGSLSESFRRTEIFKNKGARAEEEKVVEERLKKLALVLVKTGNKRFLAALSNCISDGIPALVRACLVTVAWMSSSLSPLHGCNTFQPLACSVLAAKLLDRLSYDTVMEERVLAALSLLNLVRHPECLEGLLPLKRDTTESLRDLADVTWTAKELLFACCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCD6</accession>
    <name>A0A8I7BCD6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCD6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0613360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0613360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0613360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0508540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0508540.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0508540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0613360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0613360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0613360"/>
    </dbReference>
    <dbReference type="OMA" id="RHAVSIC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0613360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="12122" checksum="1CE828BC443D1830" modified="2022-05-25" version="1">MEKAAVAAARTAHPPPPSPGKPTTLLDVHEVEWITRELERLLVRETVGAGARRRRKSAEAEAASSPASSCEAHAKKGGFLAELLGKHAASICGDGAVVSGRRRRGCGGFREVDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCE4</accession>
    <name>A0A8I7BCE4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCE4"/>
    <dbReference type="SMR" id="A0A8I7BCE4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0509700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0509700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0509700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0614720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0614720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0614720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1107">
        <property type="entry name" value="OS08G0373000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="59"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="222"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="477"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="597"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0614720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="967" mass="108688" checksum="124CD08CBD4B4B74" modified="2022-05-25" version="1">MSLRFTVNAANYKPDRGGRGVRASIQPTHHTQRRQNHLSLLSPTRASMDAALVSVATGALKPVLEKLAALLGDKYRRFKEVRDEIKFLTDELAAMHNFLLKMSEVEDEDPVVQDKAWMNEVRELSYDIEDHLDDFVARVDDDRSAKPDGFMEKIKYLLDRTKARRGIAKAIQSLKKQVVVVGKRHARYATREAISKPSNAMVDPRALAIFKDVSKLVGIDGPKDELIQLLSEPTQQQSRVVSIHGFGGLGKTTLANQVYKELVGQFDCHAFLSVSRNPDTMKVLRTILSQVTGKDYVNTKAGGEQQLITEISSFLLDKRYIVVVDDIWNIKEWNVMKYAFPITTLGSRIITTTRNIDVAQSCSSRKNIYKIKPLNVVHSRQLFCIRLFNSEEDCPSHLKEVSDQILEKCGGLPLAIIAISGLLANTESTNDQWDRVKKSIGCALERNPSIEAMIKILSLSYFDLPPHLKTCLLYLSIFPEDQVIKRKALIRRWISEGFIRKEERYTLHELGERCFNELINRSLIQPAARNTYGKVVSCRVHDTILDFIISKSIEENFVTLVGVPNLAIGTQSKVRRLSLQVKDEGNCIPPANLVLSHVRSLNVFGNTVKIPSMTEFRHLLVLDFGGCRQLENQHLVNIGRLFQLRYLNLRQVGVSELPKEIKHLQCLEMLDLRDTNVRELPTVIVNLGSLVHLLVDNCVTFPDGVSKMQALEMLKRVRALKQSSNFLRELGQLKNLRKIYLDLFDVPVAGVTKKCCKNSTAYQSLRNLGTQNLRSVTIWNGGSFLLEPWSPTPVGLKKLMTWRSAVAQVPAWVGSLVNLQKLRLEVEGLEPEDLCILGGLPALLTLDLEETTKSSKGKLSVSGGDGFRCLRNLRYNMMLMFTQGCMPRLERINIYLSIAKTESLAGGDIDTYDFGIENLPCLTSIECRVSGTTSVEGLEAAKAAMERAAMKNPNHPNLQLLNFHVGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJ88</accession>
    <name>A0A8I7BJ88_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJ88"/>
    <dbReference type="SMR" id="A0A8I7BJ88"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0698010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0698010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0698010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0579170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0579170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0579170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0698010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0698010.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF7">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0698010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="161" mass="18122" checksum="6C9BFE6FFCA5A105" modified="2022-05-25" version="1">MSSSASASRRSWPRYGEVPMIRCPACPRTAPLKRLVTTIDKNGNHGREFMKWESKQEQGKKLKQCAHFEWLDDYIERIQLEGASRELDLPLEAEKFGSGAPRSGNSIGATVGDAVVTTELKKLNKQMKKLIELKKHGNLMAVIFYVCVIAPAFVYLMIISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-02" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D2XV76</accession>
    <name>D2XV76_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="2">Universal stress protein 23267</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123428076</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-12" db="EMBL/GenBank/DDBJ databases">
            <title>Identification, localization and characterization of putative USP genes in barley.</title>
            <authorList>
                <person name="Li W.T."/>
                <person name="Wei Y.M."/>
                <person name="Wang J.R."/>
                <person name="Liu C.J."/>
                <person name="Lan X.J."/>
                <person name="Pu Z.E."/>
                <person name="Zheng Y.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GU258518">
        <property type="protein sequence ID" value="ADB54810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK371957">
        <property type="protein sequence ID" value="BAK03155.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968176.1">
        <property type="nucleotide sequence ID" value="XM_045112241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D2XV76"/>
    <dbReference type="SMR" id="D2XV76"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202640"/>
    </dbReference>
    <dbReference type="GeneID" id="123428076"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0202640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0202640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0202640"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_9_1_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="10"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADB54810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK03155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0202640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="17303" checksum="8344272D75F849CE" modified="2010-03-02" version="1">MAAPPPPPEQKMMVAIDESECSHYALEWALRNLAPRRLVLFTVQPFSPLSYLPAGSPLGPSVASPELIRSVTEHQRQLAQALVDKAKAICADHGVDAETVIEVGDPKETICEAADKLNVDLLILGSHSRGPIQRFFLGSVSNYCSHHAKCPVLVVKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTV5</accession>
    <name>F2CTV5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123411367</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK355057">
        <property type="protein sequence ID" value="BAJ86276.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960235.1">
        <property type="nucleotide sequence ID" value="XM_045104300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CTV5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0055610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0055610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0055610"/>
    </dbReference>
    <dbReference type="GeneID" id="123411367"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0043820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0043820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0043820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0055610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0055610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0055610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411367"/>
    <dbReference type="HOGENOM" id="CLU_051752_5_0_1"/>
    <dbReference type="OrthoDB" id="669838at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF39">
        <property type="entry name" value="HARPIN-INDUCED PROTEIN 1 CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="77"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ86276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0055610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="208" mass="22076" checksum="023D2353E1EFABF0" modified="2011-05-31" version="1">MPATTASARPVAVLRCIVAALVVTVLLAGLVVLVFWLVVRPKPIEYSVARAAVRHLNLTAPPGGGGGATLNASFYLTLAADNPNRRVSMRYSSVVVYVHYGAGEVAPQLAVADVPDFRQPSRNETLIQVRAVARSAPVADWVARELEHDRSDGEVGVEVRVTAVVHFLVGGVKSRHYNMRAVCSPVVIGLSPSSAKSFRGVPCDVAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2CXJ0</accession>
    <name>F2CXJ0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444223</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK356343">
        <property type="protein sequence ID" value="BAJ87561.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362308">
        <property type="protein sequence ID" value="BAJ93512.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK368652">
        <property type="protein sequence ID" value="BAJ99855.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976820.1">
        <property type="nucleotide sequence ID" value="XM_045120885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CXJ0"/>
    <dbReference type="SMR" id="F2CXJ0"/>
    <dbReference type="FunCoup" id="F2CXJ0">
        <property type="interactions" value="2114"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2CXJ0"/>
    <dbReference type="PaxDb" id="4513-MLOC_65562.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069880"/>
    </dbReference>
    <dbReference type="GeneID" id="123444223"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444223"/>
    <dbReference type="eggNOG" id="KOG2667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034705_1_0_1"/>
    <dbReference type="OMA" id="GWAIANT"/>
    <dbReference type="OrthoDB" id="270930at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CXJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF58">
        <property type="entry name" value="OS05G0490200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="149"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ87561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ93512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="383" mass="43005" checksum="1F8BE592F02EEE11" modified="2011-05-31" version="1">MDGLLSKLKGLDAYPKVNEDFYKRTLSGGVVTLLSAFVMLLLFVSETKSYFYSATETKLVVDTSRGERLRVNFDITFPSIPCTLLSVDTRDISGEQHQDIRHDIEKKRLDSHGNVIESRKEGIGGTKIEKPLQKHGGRLGKGEEYCGTCYGAEESDEQCCNSCEEVREAYKKKGWALTNPDLIDQCAREDFVERVKTQHGEGCSVHGFLDVSKVAGNFHFAPGKGYYESNVDMPELSAEGGFNITHKINKLSFGTEFPGAVNPLDGAQWTQPASDGTYQYFIKVVPTIYNDIRGRKIDSNQFSVTEHFRDGNVQPRPQPGVFFFYDFSPIKVIFTEENRSFLHYLTNLCAIVGGIFTVAGIIDSFIYHGQKALKKKMEIGKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYM8</accession>
    <name>F2CYM8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123448542</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356734">
        <property type="protein sequence ID" value="BAJ87949.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYM8"/>
    <dbReference type="STRING" id="112509.F2CYM8"/>
    <dbReference type="PaxDb" id="4513-MLOC_58185.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0308480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0308480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0308480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0370650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370650"/>
    </dbReference>
    <dbReference type="OMA" id="WRVGEFE"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CYM8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039928">
        <property type="entry name" value="LNK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33334">
        <property type="entry name" value="PROTEIN LNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33334:SF8">
        <property type="entry name" value="PROTEIN LNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ87949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="512" mass="56764" checksum="DA12FC8ECDD10643" modified="2011-05-31" version="1">MPDWKAGEFEGQFKDEFAQGHNNLQEEGAGPLNISNKKLKHGITTQENPQGVVSTATNSDSRKFKSEHIYSTCVVASDHVRDCKIESSAFPLSTDDTTSGTRYQTENWNNCQFALSNGSAVLNNCSVPQSDFSYGDNDLNFIDWPSIDNFEDVDTLFRGCDSTYGEQQLENTDELSWIPSSDAIYSSDVALQAGFGSSYSDYGMLDDLSAFPCAQDKSLPTADEQFNDNYAFIEQKNVNVYGEQAYQGDAMELLSTDQICSGDGNLDMIGERYSSENALRQLEDRKFSVASGFQLSSSQNLLKQKHHSDKTSPSNITSDCYSDRNCQFSPSGSSAQRNLIVQKQVANLQQLINDEGQLGQQTLTGRSSGPCENYEVEKKGLGKRSKDTLGTSVVVDGSFVSSLSSDNSVEESSFRQLQDAVSQLDVKTKLCIRDGLYRLARSVQNRPVFPNVMNRHEESWDVKDTQNMETSGKFVDHRSIETQTNPIDRSIALLLFHQPSDQVSGAVDNPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0I6</accession>
    <name>F2D0I6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123409793</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK357393">
        <property type="protein sequence ID" value="BAJ88607.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958599.1">
        <property type="nucleotide sequence ID" value="XM_045102664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0I6"/>
    <dbReference type="SMR" id="F2D0I6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0746000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0746000"/>
    </dbReference>
    <dbReference type="GeneID" id="123409793"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0618740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0618740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0618740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0746000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0746000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0746000"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409793"/>
    <dbReference type="OrthoDB" id="207175at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080050">
        <property type="term" value="P:regulation of seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF9">
        <property type="entry name" value="APETALA2-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="104"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="196"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ88607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0746000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="429" mass="46161" checksum="B34E195187900552" modified="2011-05-31" version="1">MHPAMAAAFCYGGEDEDAGVVVAEGGAGAGWAVFGGGGGEARPAFVTQQLFPTAAAAVTGTATQQQQQQQAVERCVLAGAAGAHWTRPLSRTKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAQAAARAYDQAAIKFRGVEADINFLLDDYKEDIGKMSLLSKEELVQVLRRQGAGFVRGSSRFRGVTLHKCGKWEARIGQLMGKKFVYLGLYDTEMDAAKAYDKAALDCCGEEAMTNFEPKAAVAACDGDLDLHSWGGEPDLELSLGCSGGGNPSTVTTGDTLRTAAAPGRQRTSLTFEMPEEEEATAPWHPGRNRSIWIRPSPTSITSGLRCPDGDHRPAFTGSSMLHMCQIGGGGREEPHMPWWPTGSGVDRWQPYGAEAAAAASSGFPPAPGLGGVQRLHQHRPGSREHLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2G0</accession>
    <name>F2D2G0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123402353</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAJ89281.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_045096268.1"/>
    </dbReference>
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    <dbReference type="PaxDb" id="4513-MLOC_67451.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612340"/>
    </dbReference>
    <dbReference type="GeneID" id="123402353"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0507720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0507720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0507720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0612340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612340.1"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008390">
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        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS02G0731500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="33"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ89281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="188" mass="19650" checksum="CB6B6D01603D30B7" modified="2011-05-31" version="1">MAVGAASRRYIGPLLCINLVMHAAVLGISGWSLNKFIDRETHRHLGGNTATGYLLVFSLMAGVVGACSVLPALLHVRAPWHSESLAAAASTGLVSWALTALAFGLACKHITLGNRGRRLRTLEAFITISTLTQLFYLLLLHAGALSGVLGVGQACGNQGETCCREIARARGELGADHKKAGGDPPPDA</sequence>
</entry>
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    <accession>F2D2U2</accession>
    <name>F2D2U2_HORVV</name>
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        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123445233</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310400"/>
    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0259200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0259200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0259200"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0310400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0310400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445233"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053304">
        <property type="entry name" value="RNA_M5U_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47548">
        <property type="entry name" value="BNAA06G32370D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47548:SF1">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="53"/>
            <end position="62"/>
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        <location>
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        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
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        <ligand>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0310400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="551" mass="61092" checksum="B4689E6FDB60C893" modified="2011-05-31" version="1">MPPPAGLIPWPSPTAPAPRITTTTRPRPPPRVRPPPPPPPPRRAPPTPPPRLKPVVAPKPAASLPPPPVIVSTMDPSSTCLNCTHFGSCSGCTHELDLDKPPVLQEVADFFSGHGIEDFTFSRGRLSEWRCRAKLAVRGTPEKPLIGLYQEGTHTVQDIPECRAHHPSINYAIKLLKQGISELNVQPFNEDAGTGELRYVQMTVTTYNTSIPVAQRYEQARVQVSLVWNSRDERSKNSEKLSLLQEFLWTNGGPRSNLHVIHSIWANFQTSTSNIIFGHKWRHIGGEADLWERFGGVDICLDPYSFGQANTLSFNSLLHKLIKYVPRGSTVVDLYSGAGVIGLAIAASRKCRSVRCVEINKMSKLSFEKSASRLPPNLGCTITWHNTDASAEPIHWLEGSSVAIVDPPRKGLHPSVINALQRVALSERKAFKAKSSLTKAKDEKRPWILRAREPAVHVDSTIMEESSGIWPETLIYISCGWDSFKKDCKSLISNEAWHLENAHAFNFFPGTDSIEVLAIFRRDSGIAQKKTKTKTKAKTKAKKKKAKPSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2D6E0</accession>
    <name>F2D6E0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401165</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">cv. Morex</strain>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
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    <sequence length="433" mass="46635" checksum="5E4BF08A4EC7C522" modified="2011-05-31" version="1">MDFEADGRFGNKRVHNRLGPAPGAASSSSSTKVCIHWRAGRCNRFPCPFLHSELPEAATTKRPNQRDGPGGHVWRNPNSGGGGRGGGGGYNKWGRGPGGADGGVRHKVPDRPCKFFLAGDCTYGEKCRYPHTYCMSNSITLLTPLQGHEKVVTGIALPAGSDKLYSGSKDGTVRLWDCQTGQCAGVLPVGGEVGCMISEGPWVFVGIPDAVKVWNMQTQAEMNLTGPTGQVYALAVGNELLFAATQDGRILAWRFSAVTNCFEPAASLTGHQLAVVSLIVGGMRLYSGSMDKTIRVWDLATLQCIQTLSDHTNVVMSLLCWDQFLLSCSLDQTIKVWAATESGNLEVTYTHKEENGALALTGMPDAQSKPVLVCSLNDNTVRLYDLPSFSDRGRIFSKQEIRAIQTGPGGLFFTGDGTGELKVWQWIIDASQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7E7</accession>
    <name>F2D7E7_HORVV</name>
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        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
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        <name evidence="5" type="primary">LOC123395434</name>
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    <organism evidence="4">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Shoot</tissue>
        </source>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML48-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML49-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
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            <end position="319"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="12"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ91018.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0461280.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="389" mass="40299" checksum="8BA9EBE96CF3697A" modified="2011-05-31" version="1">MACASRVIILPPSPVSSHKNWCQPSFTVRKPPSPPRRDSPHPIPQAPPPKLDRPTMSGYPYGGAGGGGGYGAPPPYGSSPAPSAPPYGDKPPKEAKASSPYYASPPQAYGAGGAGGGGGGYGAPPSSQPQSYGGGYGAPPPSQPQSYGGGGYGAPPSSQPQSYGGGGYGAPPSAQPYGAPPPSSASYGAPPPAAAPYGAAGGYGSPFAALVPSAFPPGTDPNVVACFQAADRDGSGTIDDKELQSALSGYNQSFSIRTVHLLMYLFTNTNVRRIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDASELRDALLSLGYSVSPTVLDLLVSKFDKTGGMSKAVEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFSYEAFMLTVLPFIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DV48</accession>
    <name>F2DV48_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123409102</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367766">
        <property type="protein sequence ID" value="BAJ98969.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958020.1">
        <property type="nucleotide sequence ID" value="XM_045102085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DV48"/>
    <dbReference type="SMR" id="F2DV48"/>
    <dbReference type="STRING" id="112509.F2DV48"/>
    <dbReference type="PaxDb" id="4513-MLOC_75447.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742700"/>
    </dbReference>
    <dbReference type="GeneID" id="123409102"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0615850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0615850.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0615850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0742700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409102"/>
    <dbReference type="eggNOG" id="KOG0715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818886_0_0_1"/>
    <dbReference type="OMA" id="QEDGDDW"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="56"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ98969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="17845" checksum="50A1B930643A3545" modified="2011-05-31" version="1">MAVAGGGVVLSLRGPSASASPAAGRLRRTSSSPSVRCGATRERWPAAAPYGALEEDHYRVLRLEPGASRGDVKKAFHRLALQYHPDVVRRQGGGDDGQESGMVEFERINAAYHTVMRNMREAEATLEYWRRRYGLADEDLDRYRHYLNDGDEDDWFADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F2E5X4</accession>
    <name>F2E5X4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123440681</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK371548">
        <property type="protein sequence ID" value="BAK02746.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973173.1">
        <property type="nucleotide sequence ID" value="XM_045117238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E5X4"/>
    <dbReference type="SMR" id="F2E5X4"/>
    <dbReference type="FunCoup" id="F2E5X4">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2E5X4"/>
    <dbReference type="PaxDb" id="4513-MLOC_72517.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309490"/>
    </dbReference>
    <dbReference type="GeneID" id="123440681"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0258390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0258390.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0258390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0309490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0309490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0309490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440681"/>
    <dbReference type="eggNOG" id="ENOG502S4YZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113093_0_1_1"/>
    <dbReference type="OMA" id="HRGIHKM"/>
    <dbReference type="OrthoDB" id="1908104at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E5X4">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47373">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47373:SF1">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019634" evidence="5">
        <location>
            <begin position="26"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="41"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK02746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0309490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="151" mass="15958" checksum="60B22B21D6408B34" modified="2011-05-31" version="1">MARVIGASGACALLVVLLVACAASAARTGPGAARQLWDDGRKVGGWTEVRDVEGDREVQELGRYSVEEHNRRREEGCEGGGAVCGRLEFARVVSAQRQVVSGVKYYLRVAAAEANGAGSNGVSDGRVFDAVVVVKPWLQSRSLVRFAPADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F2EAD0</accession>
    <name>F2EAD0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402334</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
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        <source>
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        </source>
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</entry>
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        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
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                <person name="Amano N."/>
                <person name="Kanamori H."/>
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                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <source>
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        <source>
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                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">cv. Morex</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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        <property type="nucleotide sequence ID" value="XM_045091951.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010650.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0010650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0010650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397355"/>
    <dbReference type="eggNOG" id="ENOG502STQ5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0UGL3"/>
    <dbReference type="OMA" id="EARMCKS"/>
    <dbReference type="OrthoDB" id="1480833at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00107">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.10">
        <property type="entry name" value="Knottin, scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008176">
        <property type="entry name" value="Defensin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003614">
        <property type="entry name" value="Knottins"/>
    </dbReference>
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        <property type="entry name" value="Scorpion_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147:SF132">
        <property type="entry name" value="KNOTTIN SCORPION TOXIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00304">
        <property type="entry name" value="Gamma-thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00288">
        <property type="entry name" value="PUROTHIONIN"/>
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        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57095">
        <property type="entry name" value="Scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00940">
        <property type="entry name" value="GAMMA_THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Knottins-like" evidence="3">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0010650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="83" mass="8972" checksum="EC2483A8079D445A" modified="2013-04-03" version="1">MALSRRRMAASVLLVLVLLVATEMGATRVKMAGARDCVSQSHNFKGACLSSSNCAAVCRTENFPGGECHTPHVERKCFCKRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>M0UXE5</accession>
    <name>M0UXE5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123409878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958683.1">
        <property type="nucleotide sequence ID" value="XM_045102748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UXE5"/>
    <dbReference type="STRING" id="112509.M0UXE5"/>
    <dbReference type="PaxDb" id="4513-MLOC_15347.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0732270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0732270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0732270"/>
    </dbReference>
    <dbReference type="GeneID" id="123409878"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0607690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0607690.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0607690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0732270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0732270.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0732270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409878"/>
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    </dbReference>
    <dbReference type="InParanoid" id="M0UXE5"/>
    <dbReference type="OMA" id="CYNKENS"/>
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055290">
        <property type="entry name" value="At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056592">
        <property type="entry name" value="Beta-prop_At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
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    </dbReference>
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        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0732270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="400" mass="44895" checksum="1DA1FA88819AE52E" modified="2013-04-03" version="1">MEEAGKGEVLERNTKSRLDSHPGSTASFFTDDLILDILSRLPARSVHRFKCVSVTWRDIIADSANRKKLPQTLAGFLYTTTVGWNRHHFATVSGSAAPFDTSLPCLQPNKYKDMAQVDACNGLLLYLCSSKSVVNPWAWAEDDFRFVVCNPATGRWVELPPQPQAPIYRYRYNCMAGLAFDPAVSSHFHVFCFEETTVKTYMTGVNIYSSRTGAWSRRDGGIAEKVALFFYNKGIFVDGMLYVIGNLKGTSNEYVLLGMDMEGKVQKTIPMPYGRRFGTIGSSQGCLLYAVASVDDNNKILDSEIELWCLKDFDSKELVLKHTANIDELMSMTGEKYRVVGIHPDCDTIFLVSYGGDTLVAYDIRHQKVGRILNLEKNNAQRFLPYVPLFSESLADADRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>M0W8N3</accession>
    <name>M0W8N3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401456</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044951216.1">
        <property type="nucleotide sequence ID" value="XM_045095281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W8N3"/>
    <dbReference type="SMR" id="M0W8N3"/>
    <dbReference type="FunCoup" id="M0W8N3">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W8N3"/>
    <dbReference type="PaxDb" id="4513-MLOC_44982.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0572160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0572160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0572160"/>
    </dbReference>
    <dbReference type="GeneID" id="123401456"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0474300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0474300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0474300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0572160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0572160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0572160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401456"/>
    <dbReference type="eggNOG" id="ENOG502QWF6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W8N3"/>
    <dbReference type="OrthoDB" id="666604at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W8N3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0572160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="446" mass="49030" checksum="FE9CF3E4ABFCAE51" modified="2013-04-03" version="1">MPCTSSKVAPRPSSNARPATGHSLLLLSLLQSSRALLASCSPPRRKIMAVTPRGRPDSSLGHRRPVLLLILFFCLLAFFPCRVAAFHSFVGTYGVNYGRIADNLPPPTEVVKLLRMARIKNVRIFDSDHTVLDAFRNSGLNLAIAIPNGLVKDISANPSKAMDWVNENVRPYYPSTRIVAIIVGNEILGGQDTGLAEALFGAVVNIHDALRTLRLSGRIEVNTPHSEAVFGTSYPPSAGTFRPDLMPYLKPLLDFFSKTGAPFYVNAYPFLAYMSDPEHIDVNYALMKPNAGILDQKTNLHYDNMFEAQIDATYAALEAAGYSDMEVRVSETGWASAGDATEPGATMENARTYNFNLRKRLFLRKGTPYRPKRVVKAFIFALFNEDLKTGPGSERHFGLFKPDGSVSLDLGFKGLTSSSSSIKGWNILRYSATLFSCTFIFLALST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>M0WBD8</accession>
    <name>M0WBD8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123399216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family. Cyclin T subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0WBD8"/>
    <dbReference type="SMR" id="M0WBD8"/>
    <dbReference type="PaxDb" id="4513-MLOC_4749.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0457150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0457150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0457150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0378420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0378420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0378420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0457150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0457150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0457150"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WBD8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20587">
        <property type="entry name" value="CYCLIN_AcCycT_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20588">
        <property type="entry name" value="CYCLIN_AcCycT_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000026">
        <property type="entry name" value="Cyclin-T1-5 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000028">
        <property type="entry name" value="Cyclin-T1-5 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="68"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="183"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="520"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0457150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="543" mass="60740" checksum="53772F128472AE60" modified="2013-04-03" version="1">MAMLPSDSSHHRIVENSPYRITKSGNKKAGKLSASWYFSRKEIEENSPSKRDGIDLKKETYLRKSYCTYLQDLGMRLKVPQVTIATSIVFCHRFYLRQSHAKNDRRTIATVCMFLAGKVEETPRPLKDVILVSYELIHKKDPAAGQKIKQREVYDRQKELILLGERVVLATLGFDLNVHHPYKPLVETIKKFKITHNALPQVAWNFVNDGLRTSLCLQFKPHLIAAGALFLAGKFLKVKFLPDDGEKAWYQEFDVTPRQLEEVSNQMLELYEQNRVVQSQPTNGSEAEGSSAGVPNQRISVKTAANSEEPPVVHHPASKQSDSSASTPTGVPIHNGAERSKPHKHIGSHRSLQNDNGDHENDKISSQSGGRVDTSTKDGLHDGTKSLPISSRPSDKPGTPTEEEKPLPAHASSSESRDGNLTNSGDPSVSSSMMDAMNKIDKDKVKAALEKRRNSKGYVARKVDVMDDDDLLERELEHGVELAVEDEKIKQERDHCSVHQEDHRNADDVAENGDNNGEQNVRETAKEAEVLVNIKDERSPTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0XCW7</accession>
    <name>M0XCW7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123410903</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044959768.1">
        <property type="nucleotide sequence ID" value="XM_045103833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XCW7"/>
    <dbReference type="SMR" id="M0XCW7"/>
    <dbReference type="PaxDb" id="4513-MLOC_58950.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0052450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0052450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0052450"/>
    </dbReference>
    <dbReference type="GeneID" id="123410903"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0041130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0041130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0041130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0052450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0052450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0052450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410903"/>
    <dbReference type="eggNOG" id="ENOG502QPKD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1931139at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XCW7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000002">
        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF208">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0052450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="335" mass="36796" checksum="B2DA1A74E17252E3" modified="2013-04-03" version="1">MGTMTLPPGFRFHPTDDELVGYYLKRRVDNLKIELEVIPVIDLYKCEPWELPEKSFLPKRDLEWFFFVPRDRKYPNGSRTNRATTTGYWKATGKDRKVSCDGGAVCGVRKTLVFYKGRAPGGERTDWVMHEYRLCQDLLHGASNFIGAYALCRVIKRTEAGLLHGDAAAKAKPCHQQMTKVGSSSSLVTTDQQLGSLTPSPPRLDIVGNAFQLHTSPSPLYGGEVVSGMGAPLGFPQQDAAATFFVDADLAGAGETTQSHMPFFGDMGVVSDHELRWDTLPPYANNTFSTTAVAGAAELWNPAAPNAAAPMLCREESDDLAAFFSSLDENNMVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MYD0</accession>
    <name>A0A287MYD0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123450993</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983939.1">
        <property type="nucleotide sequence ID" value="XM_045128004.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983940.1">
        <property type="nucleotide sequence ID" value="XM_045128005.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983941.1">
        <property type="nucleotide sequence ID" value="XM_045128006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287MYD0"/>
    <dbReference type="SMR" id="A0A287MYD0"/>
    <dbReference type="STRING" id="112509.A0A287MYD0"/>
    <dbReference type="PaxDb" id="4513-MLOC_42946.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333850"/>
    </dbReference>
    <dbReference type="GeneID" id="123450993"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450993"/>
    <dbReference type="eggNOG" id="ENOG502QWBY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287MYD0"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287MYD0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052610">
        <property type="entry name" value="bHLH_transcription_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45959:SF53">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45959">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="365" mass="38872" checksum="C4289DABA8863CE3" modified="2017-11-22" version="1">MDDYSALFMEWAMDTLEQEHPDPVVIVNGDCGEAAFPSLQALREQRLVLEELITGTNPASSGSSGETTDGSVGCGGNFSSPAAMEHDVWPPSPPTSARRAPRLSMKAGGTSLPVTSWNFCAASALPASDDGTLDSGSAGPAVPEMVYGSQPTRRAAARSPTGTGPVSSGPPYAQDHIMAERKRREKINQRFIELSTVIPGLKKMDKATILSDATRHVKELQEKIKALEAATGRSSRSIETVVLVKKPRNAAVSDQNGSPSSASSGAPASRNRLPEIEVRFSENGVMVRIQCDDVKGVVVRVLSVVEQGLHLSVTHANVMPFTPCTVIITITAKVDEGFTVTAEEIIGRLNYALEQHTSCTSTEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287NV79</accession>
    <name>A0A287NV79_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Retinoblastoma-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123448537</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Regulator of biological processes that recruits a histone deacetylase to control gene transcription. May play a role in the entry into mitosis, negatively regulating the cell proliferation. Formation of stable complexes with geminiviridae replication-associated proteins may create a cellular environment which favors viral DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981402.1">
        <property type="nucleotide sequence ID" value="XM_045125467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287NV79"/>
    <dbReference type="SMR" id="A0A287NV79"/>
    <dbReference type="STRING" id="112509.A0A287NV79"/>
    <dbReference type="PaxDb" id="4513-MLOC_57590.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370540"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370540.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370540.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370540"/>
    </dbReference>
    <dbReference type="GeneID" id="123448537"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0370540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0370540.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0370540.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0370540"/>
    </dbReference>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287NV79">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000030">
        <property type="entry name" value="Retinoblastoma-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000075">
        <property type="entry name" value="Retinoblastoma-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF22">
        <property type="entry name" value="RETINOBLASTOMA-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08934">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="10">
        <location>
            <begin position="67"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="11">
        <location>
            <begin position="389"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="845"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="952"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="851"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0370540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="980" mass="108751" checksum="2A7A964E10AB2EAF" modified="2017-11-22" version="1">MEPQPPPPPAVETHFADLCKELGVDEGVASEAAALLEEGKGVFLTPPLFGSKSPEDVERLCFAFVLYCVAKLKGKGMKEESSRVRLWKILEGCKLNYNDFFKGSQQLLSKLDHVLRSRYGTDWEDLLELKQLQSLVNLLADASRFYCKAFNELFLSASTGQEPGSTTSNPDFFCFGWLFFLALRSKSPELFKDLVSCIHGLVAILAILLIHVPAKFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSENRLKEMMGKFHKAIEEFFSTKAVRASECKTETSGKIDTDGLIYFRDLLDKECFHSNFEKLEKLTTTTSWEGELDLEMFLVNNGNIISAENSSRDFSNLSCPKRVFETLASPAKTIKNMLTVPSSPSSPANGGSVKVVQMTPVTSAMTTAKWLHEVISSLPEKPSTKLEKFLSSCDTDLTSDVTKRVSIILEAIFPTKPSGHCGGSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAVCRAESLNTNVNNLTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEESLPRELKRHLNSLEEQLLESMAWEKGSSLYNSLIVARPSLASEINRLGLLAESMLSLDDIVARQNFHAEDLPATPSKKQAADPDENGDTRSPKRLCIESRSTLVDQIPQTPSAKQSYTLKAKWCPLQSTFASPTVSNPVGRNEKCAEVGVHIFFSKILKLAAIRIRNLCERLGHVEQTERVYNIFKQILDQQTALFFNRHIDQLILCSLYGVAKVSQLTLTFKEIVNNYKREQQCMPEVFRSVFVVNTDHNGGLGSRHVDIIIFYNEVFVPAVKPFLVALIPSGAHPDNKKNHNSQIPGSPKSSPFSNLPDMSPKKVSSSHNVYISPLRQTKKDALLSPSSRSFYACIGESTHAYQSSSEDLAAINNCLNYTTRRINTRINFDMVSDSVVAGSLGQPNGVPASSDPAGSFSFLSRKGNPGPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287QGJ3</accession>
    <name>A0A287QGJ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287QGJ3"/>
    <dbReference type="SMR" id="A0A287QGJ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0436510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0436510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0436510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0361260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0361260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0361260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0436510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0436510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0436510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sugar phosphate transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0436510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="247" mass="26990" checksum="8BCBA738944BF1B5" modified="2017-11-22" version="1">MNIVLGNVSMRYIPVSFMQTIKSFTPATTVILQWLVWSKHFEWRIWASLVPIVGGILLTSMTELSFNIFGFCAAMIGCLATCTKTILAESLLHGYKFDSINTAYYMAPFATMILALPSMLLEGGGVIDWFYTHDSAFSSLIIIPGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVVVVVLVSWLIFRNPISPMNAIGCAITLVSCTFYGYVRHLISQQKAVAPLGSQGTNSSRSGVEMLPLVGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287TVG2</accession>
    <name>A0A287TVG2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123401081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950731.1">
        <property type="nucleotide sequence ID" value="XM_045094796.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950732.1">
        <property type="nucleotide sequence ID" value="XM_045094797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287TVG2"/>
    <dbReference type="SMR" id="A0A287TVG2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0572950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0572950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0572950"/>
    </dbReference>
    <dbReference type="GeneID" id="123401081"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0474990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0474990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0474990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0572950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0572950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0572950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401081"/>
    <dbReference type="OrthoDB" id="19639at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287TVG2">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF6">
        <property type="entry name" value="DITRANS,POLYCIS-POLYPRENYL DIPHOSPHATE SYNTHASE [(2E,6E)-FARNESYLDIPHOSPHATE SPECIFIC]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0572950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="245" mass="27626" checksum="99F4038474D401DC" modified="2017-11-22" version="1">MQISWSSMIVELILGLLWHLVHLLVSLFDLWSHLNDNLECYFISSELLPKYRDLHFERLNYVGVVVDSREANNVLKIKQLLRWLSNIGVKYVVLYDNEGVLKEWLKPGIEAPFLTDESSRTNLDLSAHGGMAIECLSGSDGKEGIAKAANLLCSDFGNCNSHGHKKDDNAFTEADIVCALRAVGCGGPEPDLLLVYGPVRCHLGFPAWRLRYTEIMHMGSLKSMKYGSIVKALHRFSHKYQNYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287U2E2</accession>
    <name>A0A287U2E2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123406199</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045099672.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955608.1">
        <property type="nucleotide sequence ID" value="XM_045099673.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0583650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0583650.1"/>
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    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0583650.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0583650.2"/>
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    <dbReference type="GeneID" id="123406199"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0583650.1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0583650.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0583650"/>
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    <dbReference type="KEGG" id="hvg:123406199"/>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom"/>
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        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
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    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="469"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0583650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="698" mass="80463" checksum="EFC034980EF4745E" modified="2017-11-22" version="1">MAFREERYVRFHDWRSEYSVGSDKIVSEGRHTAFDSLKDKTLGAFSFLGNSSRPETLNKSTPEERKAKTRVLDPQGPFLQRWNKIFVISCLIAVSVDPLFFYIPVIDGVKNCLYLDKKLATIASILRFFTDIFYLLHIIFQFRTGFVAPSRVFGRGVLVEDTFAIAKRYLTTYFLIDFLAVMPLPQVFVLLVLPHLKDSKVMEAKDILMVIVTCQYVPRLVRIIPLYLQITRSAGIITETAWAGAAFNLLIYMLASHVFGALWYLLSIQREDTCWRKECGKINCNFASLYCGSNTARDNIFLQNVCLTNGSDDIDPTFGIYLPALQTVSQSTSFFEKFFYCFWWGLQSLSSLGQNLKTSTYTWENLFAVFVSISGLVLFALLIGNVQTYLQSASVRIEEMRVKRRDTEQWMAHRLLPENLKGRILRHEQYRWQETRGVDEEGLLMNLPKDLRREIKRHLCLSLLMRVPMFENMDEQLLDAMCDRLKPMLYTEDSCIIREGDPVNEMLFVMRGYLESMTTNGGQSGFFNSNVLKGGDFCGEELLTWALDPAAVSNLPSSTRTVKTLSEVEAFVLRADDLKFVATQFRKLHSKQLQHTFRFYSQQWRTWAACFIQAAWHRYCRKKLEDSLFEKEKRLQAAIVSDDSTKLSLGAALYASRFAGNMMRILRRNATRKARLQERVPARLLQKPAEPNFFAVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W639</accession>
    <name>A0A8I6W639_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysine-specific demethylase JMJ706</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123436567</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_044971575.1">
        <property type="nucleotide sequence ID" value="XM_045115640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W639"/>
    <dbReference type="SMR" id="A0A8I6W639"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053590"/>
    </dbReference>
    <dbReference type="GeneID" id="123436567"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0041980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0041980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0041980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0053590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123436567"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032452">
        <property type="term" value="F:histone demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048439">
        <property type="term" value="P:flower morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000016">
        <property type="entry name" value="Lysine-specific demethylase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF107">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE JMJ706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjN" evidence="9">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="10">
        <location>
            <begin position="250"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="706"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="842"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="729"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="96775" checksum="22378AB4A7DB5A98" modified="2022-05-25" version="1">MVEGRNCLPAEVRNGLETLKKRRLERMRLSAQKDEGDNPAMAARSGGDSLRTPANCGVRLHANNVTEGLPSTSSAQNNNPFAKRKVDKFNMSNLEWIDNIPECPVYCPTKEEFEDPVAYIQKISPVASKYGICKIVAPVSASVPAGVVLMKEQPGFKFMTRVQPLRLAEWAEDDTVTFFMSGRKYTFRDYERMANKVFSKKYSSASCLPARYVEEEFWREISSGKMDFVEYACDVDGSAFSSSSRDQLGKSNWNLKNFSRLPSSVLRLLQTPIPGVTDPMLYIGMLFSMFAWHVEDHYLYSINYHHCGAFKTWYGIPGDAAPGFEKVASQYVYNKDILTGDGEDAAFDVLLGKTTMFPPNILLDHNVPVYKAVQKPGEFVITFPRSYHSGFSHGFNCGEAVNFAIGDWFPLGSLASKRYALLNRTPFLAHEELLCLSAMLLSHKLSDPKTINSEHPYTQYCVKSSFVRLMRMQRRTRSLLAKMGSQIYYKPKMYSNLSCSMCRRDCYVTHVSCGCTFDPICLHHEQELRSCSCKSDRIVYVREDILELEAIYRKFEQDIRLDKETSANISYKQAAISDIGVDHGPSVGTDQDISNSEANLLEANAADCGKSSPATSSLTSFALRDGSLPAEPKVHAARTDQIWSIAKQAIKTSSVEGNGALDGNSSCMADACNEISSCNASPMEYSGNSDSDSEIFRVKRRSSILGRSAPDTKTTNLSEQKVLKRLKKASPETQHENKRHEEDSERTSVPSVSRRHNKSNSGSSEEDREDMVPIAWRMKRRQLEAQQGDTSYAALQSKAYPSTGSCSRQQFAEATKDAASEVRPKRVKIRLPRSAANRLVEGRQQQVSSGQGFAVDDKPPGFWHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDE7</accession>
    <name>A0A8I6WDE7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123426304</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966047.1">
        <property type="nucleotide sequence ID" value="XM_045110112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDE7"/>
    <dbReference type="SMR" id="A0A8I6WDE7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0154270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0154270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0154270"/>
    </dbReference>
    <dbReference type="GeneID" id="123426304"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0127090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0127090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0127090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0154270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0154270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0154270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426304"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000076">
        <property type="entry name" value="Homeobox protein knotted-1-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005539">
        <property type="entry name" value="ELK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03789">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01188">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELK" evidence="9">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="250"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0154270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="349" mass="38575" checksum="A4CBE065F947C10D" modified="2022-05-25" version="1">MDRSSFGNLAAGGGGSSSSKSSPSFLQLQPLSTTTVAAGPGPMGGTYYGTPLALHQGAAAAGSSQSPYQFHHHKSSVGEISQAEAEAIKTKIMAHPQYTALLVAYLDCQKVGAPPDVLERLTAMAAKLDAHTPGRLHEARDPELDQFMEAYCNMLAKYREELTRPIEEAMEFLKRVEAQLDSITGGGHGSAPLSLAAGKYEGVGSSEDDMDASGRENEPPEIDPRAEDKDLKYQLLKKYSGYLSSLRQEFSKKKKKGKLPKEARQKLLHWWELHYKWPYPSETEKIALAESTGLDQKQINNWFINQRKRHWKPSEDMPFVMMEGFHPQNAAALYMDGQFMADGMYRLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDP6</accession>
    <name>A0A8I6WDP6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDP6"/>
    <dbReference type="SMR" id="A0A8I6WDP6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0015430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12203">
        <property type="entry name" value="GT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000032">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654">
        <property type="entry name" value="FI21293P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654:SF66">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR GT-3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="192"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="467" mass="52298" checksum="22F4B8AE41EE592A" modified="2022-05-25" version="1">MSHFKILRDIVSSKSVMSKQQNPFFVPCHSFWTGYASMWAEGMHARPSRREGKGREGKRGTRFMLLQRGLAAQRIQILNSKAVGKQAVSALKQIDDFLFLSLSISTPLDFPFLSLSLSLVFPTLLRFPVHRHRRHHRPGQAMDPFHHLNTAFSNPYHPLLPSSPPHHHYPPPPPLQPPPAADPPERERLPQWSHAETAAFLAIRADLDHSFLSTKRNKALWEAVSARLTAHGGGFARTPDQCKSKWKNLVTRFKGTASDTHAEGGDPHVGAAARGGFPFHDEMRRIFDARVERARALEAKKAKGKDAAARRDPDDDGGGGEGDEGEDEDEEAEVFGDEEEGRADAEARGAGKKRRRKAAAAARTAAAAGGGGIEIGEVEAVLREFMRRQMEMEERWMEAAEARDAERRAREEEWRAAMVALGEERLALVRRWREREDAWRARAEEREERRHQLVAALLAKLGGDTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WKM1</accession>
    <name>A0A8I6WKM1_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKM1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110520.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110520.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="16160" checksum="DE1F4EEC77A99EAE" modified="2022-05-25" version="1">MNFPDVHTLQQALDLAPPPRLNSAQDRAEHTALQRRLLVAQEDERVMAEWRRRHPEDVAYEQEYWERRREEDTRRRREERLDRRRRKALTCAQADLVNAGGRSFFTEEDERWFDIWLSTSDDTNDDDDGADDSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WKN1</accession>
    <name>A0A8I6WKN1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123429422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044969391.1">
        <property type="nucleotide sequence ID" value="XM_045113456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKN1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110920"/>
    </dbReference>
    <dbReference type="GeneID" id="123429422"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0090980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0090980.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0090980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0110920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0110920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0110920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429422"/>
    <dbReference type="OMA" id="HEYAYSP"/>
    <dbReference type="OrthoDB" id="780868at2759"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR039609">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179:SF78">
        <property type="entry name" value="OS07G0686600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0110920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="237" mass="24449" checksum="0EDC3C1AD1B8864E" modified="2022-05-25" version="1">MDAMSCLAPPTQAQFLSASTSPGAAYYADAAIARALHFSTMPEYEYEYDYSPAASSPSSASAPSSSSLLADFPYGAGDGSSWFASTAAPPTVSLTCDSVLVSSDATPPRPPSTPAAAISAAANKRRAGVGPNAGRAGKRRARASKRAPTTYISTDPANFRLMVQHVTGVQAEPGAADGSGPFDLSSGAALLGCHASSDADADAAALHRHHQQQLAQQQCYPTLDSWSVLYESSQQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM42</accession>
    <name>A0A8I6WM42_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WM42"/>
    <dbReference type="SMR" id="A0A8I6WM42"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0107550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0107550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0107550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0130310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000002">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] iron-sulfur protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="219" mass="23891" checksum="BCB4E916DF282D06" modified="2022-05-25" version="1">MATGPNGRAQSESNGHPYTALGSIIFSVRPDRTFAGRLLRSSSAPHLSFRPHLSPAHGSDAMAAPLRRVFPSLGRALLSPTPARMLSAEASDALVEIKRGEIGMVSGIPEEHLRRKVLIYSPARTASQQGSGKVGRWKINFVSTQKWENPLMGWTSTGDPYANVGEAGLTFDSAESAKAFADKHGWEYVVRKRHTPLLKPKTYAENFKWKGPPKTEEAA</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
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        <location>
            <begin position="108"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15694" checksum="41D723A05E4C2EE0" modified="2022-05-25" version="1">MVLVSAPVGPRPVYDSDVEVCEPEIPTHLEMLLEKVTLVEKENKYLKEKLKIIEDKNMEMELHVAEVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHSALGSIVALLAILIAFVVAFKCFT</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="170" mass="18390" checksum="6C84C9E255A7F54E" modified="2022-05-25" version="1">MSSSSYVVVPRCSVIFDGTNYGEFVGFMRIHMRGLLVWGVLSGVVPCPPCPLAHVAPTPPVLPVLAADASQADRDVAKALDDAAVDAYDQQVSSYLDALSVYRNDLSAYTQWCNDDAPAVVVLTASVLPQFPSEFMGLGTVAAMWSYLCQRHQPSGNALYLGPVWNHNRL</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <sequence length="216" mass="25227" checksum="A3A4FBDBCE19A88B" modified="2022-05-25" version="1">MPTRFEKVLAHGTTMLDVVYTNESREMPFFLEQLKERWRDAAMDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRNGITFTTVDIRNDQLKMRYSFGIEIPTGCLIDLQTVFRLRHVRTSMAHMAVALIDEEYGDMKTSFPKSQHKLREKAPLDRINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
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            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    <dbReference type="AlphaFoldDB" id="A0A8I6WTX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0293440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0293440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0293440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0293440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0293440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0293440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0293440.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19968" checksum="7DE4D63EAB9E1E66" modified="2022-05-25" version="1">MLRMDDSEDEEEERNLGKPEGTKKGKLRVKMEGEASSLREKMEQMMKAREELTTKTLETKLLITEKKKEVKLAQVEAKHEEAKRKADLEERMIKLKEAKVWKELMMEEKEHMMMSKKDMDQEQLQWWKDYKEDIAERKRIFHGASSTLRGDTPMSGGGDGGVEDSTTGDNGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUH4</accession>
    <name>A0A8I6WUH4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidophosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123444768</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUH4"/>
    <dbReference type="SMR" id="A0A8I6WUH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298790.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0249020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0249020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0249020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0298790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298790.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298790"/>
    </dbReference>
    <dbReference type="OMA" id="VFSYIPN"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="152"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298790.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="615" mass="66251" checksum="5F6A8E82FC1FD0D1" modified="2022-05-25" version="1">MSFSFTHEDKNDDRSQIPWNHSCVGHTASTRLAATSLNPVRSRTFGRASSKALTSPSQSRRVPGMAAAATATAVPPATASSNLLFTPPAPSPVHGQLRCHAGPSPSLLALRHRAARAVPARALFDKVTPFNFGDGDRPIEEDDGDDHPREECGVFGVVGDPEASSLCYLGLQKLQHRGEEGAGICAAGDDGKLKSVTGLGLVSDVFRDPAHLKSLPGQAAIGHVRYSTSGGNAQLCNVQPFLAGYRFGQLAVAHNGNLVNYMPLRNKLEAQGSIFNNSSDTEVILHLIATSLSRPLLARICDACERLQGAYSLLFLTADKLFAVRDPFGFRPLVMGRRPNGAVVFASETCALDLIDAVYEREVEPGEVIVVDRRDMSVSSACLVPHRPRKSCVFEHIYFALPNSIVFGHAVHERRTAYGRALAEESPAPTADVVIPVPDSGFYAALGFAQASGLEFQQGLIRSHYTGRSFIQPTQAIRDLAVKLKLAPVRGVITGKSVVVVDDSIVRGTTSSKIVRLLRDAGAREVHMRISSPPVVGRCHYGIDTPDENELISNRLDIEGVRKMIGCDSLGFLTLDKLHSIYGDEADELCDACFSRKYPVDPPEKVPAMASADED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYG5</accession>
    <name>A0A8I6WYG5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYG5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0253300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0253300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0253300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0253300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26339" checksum="6C3AD3332BC7B99E" modified="2022-05-25" version="1">MLVRMMSVADYRWYPEYTVEEQYRDFNQSQYLCTVRVFPDYPRAEEPIHWSCGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDTLLQSTAEMLENRDRDARALRMELYATCARVWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDGPTIGGVAPLRGPLLPPIKGPRPHSCPYGSQGSQARAFPDPQVELPGHEANLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZZ8</accession>
    <name>A0A8I6WZZ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123406237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0337540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0337540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0337540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0405450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0405500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447117"/>
    <dbReference type="KEGG" id="hvg:123447121"/>
    <dbReference type="OMA" id="FRSLACH"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000167">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0274">FAD</keyword>
    <keyword evidence="3 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="529" mass="59346" checksum="13B377BF5F53851F" modified="2022-05-25" version="1">MAQGQEARATREAVPPVSRVAIIGGGISGLAAAKQMAAYDPVVFESTPSVGGVWKHCVYRTTRLQTPRPDYEFSDYSWKNRDDPSFPTHTEIVDYLEGYADEFDLWRYISFGSKVVDIKFLGGAEAGFTELWSGTGKAPLRGKPMWEVGVVTGDSNIVQYYKFEFVVMCTGKYGDVPRMPVFPPGKGPEVFKGTVMHSLDYCKLSEEETVELMRGKKAVVVGYKKSAIDLANECAQANQGEGGQACTMLVRTLHWVVPSYSIWGLPFFMFYSTRFSQLFYERPNQGFFRSLLCRLMSPLRAGVSKFIESYLSWKLPLGKYGLTPDHPFVEDYASCQMAILPEGFFDMADRGLVRFKRASAGWCFSENGVVLDDGTKVEADLVFLATGFEGKDKLREVLPKPFRDLVVGKSSMMPLYRGTIHPLIPNMAFVGFVESVSNLHTSELRCRWLSGLLEGRFELPTVKAMMGHVAGEADAMRRTTRFYRRHCISTYSIHDSDGMCADLGSATLRKANWIAELFAPYNNKDYKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4X8</accession>
    <name>A0A8I6X4X8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123413626</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044962498.1">
        <property type="nucleotide sequence ID" value="XM_045106563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4X8"/>
    <dbReference type="SMR" id="A0A8I6X4X8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069720"/>
    </dbReference>
    <dbReference type="GeneID" id="123413626"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413626"/>
    <dbReference type="OrthoDB" id="638990at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035306868" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="32"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="22810" checksum="8A1CDE47548E4D75" modified="2022-05-25" version="1">MAPTAAGARVPLAVTVFVAVVSSMLLAPAAADLDGDRDALMAMRSGLQDPDDALYTWTPELNTCDWSYVTCDGQANRVTEIKIGHKNLSGPLPPELGKMDQLKKLWISWTNIQGTIPEELGDLENLKSLHLHNNTLSGQIPASLGKLKSLEQLHLQQNRLTGPIPSELAGLPDQMSVDLSSNDLCGPIPTHGPFNNIHPPNGLADNPRLGGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6G2</accession>
    <name>A0A8I6X6G2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6G2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0189030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0189030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0189030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0156530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0156530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0156530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0189030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0189030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0189030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053313">
        <property type="entry name" value="RGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34961:SF11">
        <property type="entry name" value="OS04G0554900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34961">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0189030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="131" mass="13829" checksum="7E7FC7D59C06A05B" modified="2022-05-25" version="1">MPRLSSTLAPTRQRSVAATSSVLVTLFFLALFIGSCEPRRLLVHGATATSSEPPPAPCKTKHLSTADVKDPCVDHFGTRVKAGGVAIPSAAARTAAVGRVSRQLSHRDLQVVGTAFHLDYAGPETHPPIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X828</accession>
    <name>A0A8I6X828_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">riboflavin kinase</fullName>
            <ecNumber evidence="2">2.7.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444449</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6X828"/>
    <dbReference type="SMR" id="A0A8I6X828"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0240850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0240850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0240850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0289970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289970"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000119">
        <property type="entry name" value="Bifunctional riboflavin kinase/FMN phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF44">
        <property type="entry name" value="OS01G0757900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="9">
        <location>
            <begin position="313"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="433" mass="47825" checksum="A0482AD2587C9B88" modified="2022-05-25" version="1">MDSDSTYPLLVGLLPPRTLNPTTSLPPTQTRQSPPCIASPRLLRASSMAATHRVSAVIFDLDGTLLDTERATRDVLKEFLGAYGKVPDAAKEEKRLGQMHRESTTGIIADYGLPFTVEEYSEAIYPLYIKRWQRSSPLPGVNRLLKHLHKNGVPLALASNSIRRNIDHKILKLGELKDCFSVVLGGDQVPHGKPCPDIFLEAAKRLGVNPSSCLVIEDSLVGVQAAKASGAKVVAVPSLQSQRHCYSIADLILYSLLDFHPELWGLPPFEDRIQCALSMEPLLSNAQIGDVVLNNTHTIRAGEYTYDSIPDQISGIFFGWAKSETYGVFKAVVSIGWDLSPGKFERVMHICFLDSHYKTKTEDRLELLLIGYIRKLQSLENISQAMSITDEDQAIARDALDLPAFSEYAKARRALDIPASSEYAEPRNGLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X886</accession>
    <name>A0A8I6X886_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123396363</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X886"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0433070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0433070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0433070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0433070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0433070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0433070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010305">
        <property type="term" value="P:leaf vascular tissue pattern formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010087">
        <property type="term" value="P:phloem or xylem histogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013666">
        <property type="entry name" value="PH_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040269">
        <property type="entry name" value="VAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008546">
        <property type="entry name" value="VAN3-bd-like_auxin_canal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351:SF18">
        <property type="entry name" value="VAN3-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05703">
        <property type="entry name" value="Auxin_canalis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08458">
        <property type="entry name" value="PH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VAN3-binding protein-like auxin canalisation" evidence="2">
        <location>
            <begin position="86"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Pleckstrin-like plant" evidence="3">
        <location>
            <begin position="424"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0433070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="56593" checksum="68B8AB90F08FDB17" modified="2022-05-25" version="1">MHTHAWTPLSSSSRQSTHPHTHMYKTAHSPKSQALSSGRSITSSSLPRTTFPRAPPTRTHPSTAAAAAMNGSGRASARGGGDRLPPDPQRDPLEFLSRSWSASAADVSRAFAAAPAAGAIAEDIAGELDDAASAGTGTGSSFSFASAATSQLIMDRIMSHSQEVSPLTSGRLSHSSGPLNGGGSFSDSPPVSPDIDDSRMQFCRAVSTPKAQPPRGGSKTVGRWLKDKKEKRKEETRAHNAQVHAAVTVAAVAAAVAAAAAATAGSGGKDDRNARTDMAMASAATLVAAQCVEAAESMGAEREHLAAAVGSAVNARTPGDIVTITAAAATALRGAATLRARVQKEAWNVAAVIPVEKGSMGMGGHSHGQKHGAAQRQQQQQHHKVQEMGSSNSSSFSDDLPAIDQDENNFLGICCQELLARGTELLKRTRKGSLHWKVVSVYINRTGVVMLKMKSRHVAGTITKKKKSVVVDVCRDLAAWPGRHLLEGGEHRRYFGLRTAEHRVIEFECASQREHDMWTKGVARLLAIVDGRKRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDE6</accession>
    <name>A0A8I6XDE6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123441152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973592.1">
        <property type="nucleotide sequence ID" value="XM_045117657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDE6"/>
    <dbReference type="SMR" id="A0A8I6XDE6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0328100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0328100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0328100"/>
    </dbReference>
    <dbReference type="GeneID" id="123441152"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0328100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0328100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0328100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441152"/>
    <dbReference type="OrthoDB" id="654502at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09612">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033734">
        <property type="entry name" value="Jacalin-like_lectin_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="5"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0328100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="15476" checksum="302B3A458AC8477B" modified="2022-05-25" version="1">MRKPAIKIGPWGGTGGSQRDVQQKPSRLVSITIYSGGAIDSISFTYVSGDCEQHLSSGKWGGSGGSPHTISLDPTNYVTEISGTIGDCNGYNVIKSLTIVTLKEGPRTYGHVYGTPFRVPVLDGGKIVGFFGCSNAYLDAIGVYVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEM1</accession>
    <name>A0A8I6XEM1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEM1"/>
    <dbReference type="SMR" id="A0A8I6XEM1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0228250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0228250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0228250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0275490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF153">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="384" mass="42042" checksum="47B21F0103ABAA7A" modified="2022-05-25" version="1">MACSPLYKPQHALPAPVYPPTSFIAVLSLCLHLIQRQEFVSRESSDMQEALVSPANADRLRSAFELKPYAFGDQRLSSSPRYLPSGDDGLYRCSSPFSPSLGFSSPSPLATSVSLSPSSSGSLVDDGDYDGAAAADATGHRLQLARLALQYQEVADRYEMCLSHLAEAVDEAAVLRRENTELRVANSDLARRVALLGGKETAAVVIADEIRRFRLGEHKASKQRAPEKLAVLPKSISVRSNDYLKMNQAAPAAATPPAYSRKPRAANVNPSPVTQRAYLGLGVDGGKKGEEHKAKQDAAGELDVYNQGMFKTELCNKWEETGACPYGDQCQFAHGVSELRPVIRHPRYKTEVCRMVLNGEVCPYGHRCHFRHSLTAAERLLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHU9</accession>
    <name>A0A8I6XHU9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHU9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0155100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0155100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0155100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0127830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0127830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0127830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0155100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0155100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0155100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0155100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="13496" checksum="AFFC809B156E29BB" modified="2022-05-25" version="1">MGFQLSTFCFEILRRVALWRAHSIYDFTFMDVDKILPFCNTLGWHSLNVNGEVEKRKGLRWIPRHPETRKGVASDEMLRGVENKHRSGDSQIGQPFKLPAESMRRQETTWRTETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XKE0</accession>
    <name>A0A8I6XKE0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044949901.1">
        <property type="nucleotide sequence ID" value="XM_045093966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XKE0"/>
    <dbReference type="SMR" id="A0A8I6XKE0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432360"/>
    </dbReference>
    <dbReference type="GeneID" id="123399564"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0359200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0359200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0359200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432360"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399564"/>
    <dbReference type="OMA" id="DCYFDTA"/>
    <dbReference type="OrthoDB" id="1729737at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01461">
        <property type="entry name" value="vWA_interalpha_trypsin_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46503">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46503:SF1">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="330"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="752" mass="82855" checksum="F172700F8FB83D93" modified="2022-05-25" version="1">MEEDFERAVEDGLRLSKRLVLPGGAGPPRPHAAMDRYGAPEAATSLLPVAPMAYAVVTDPAAVDSPDVPSYQPHVYGRLSPPALIPLQMREVELRVDCAAGCATAEVTLVARWWLHCLTRSRDCDCRLVVPMGEEGSILGAEVTVGKKSYNTHVIDVDGNSPVKIVKTESGGILKRQFFQLTIPQVVGGEDIFATIRWSQKLLYDNGQFSVDIPFRFPHYVNPLPKIFTKKEKIQLTVNSGVSKEVLLQGTSHPLKEKIRQAEKLFFLHEAVVENWSIKDFNFSYSVYSGDVSGGVLVQRSTLRDYDDRDIFSIFLLPGNNQNRKIFRKAVVFIVDTSGSMQGKPIENVKNAISTAVSELEEGDYFNIVTFNDELHSFSSCLEKVNGKTTESAIDWMNSKFVAQGGTDIMHPLTEALALLSNSHGALPQIFLVTDGSVEDERNICHTVKTELTNKGSISPRISTFGLGSYCNHYFLRMLASIGKGHYDAAFDTGSIEGRMLQWFQKASSTIVADISIDVTKHTQEFEVDCEYIPDVSAKYPLFVSGRFRGELPETLYAEGYLSDMSKISIELKVQQIKDIPLDKVLAKQQMDLLTAKAWLSENKQLEQMVAKMSIQNGIPSEYTRTVLLQTIMEKIDPAQQGKQKPKKPEEPSAMPLQGLTLGFGNVPATTENLTSGFGDTRAPDKFDMFGKAVGGCCGRVADCCCCMCFINCCSKMNDQCAIVMVQMCAALSFLGCFECCSELCCGGGSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLC5</accession>
    <name>A0A8I6XLC5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLC5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0365400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0365400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0365400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441400"/>
    </dbReference>
    <dbReference type="OMA" id="YERNYIV"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056592">
        <property type="entry name" value="Beta-prop_At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34591:SF12">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34591">
        <property type="entry name" value="OS03G0653100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24750">
        <property type="entry name" value="b-prop_At3g26010-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035279644" description="F-box domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="85"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="540" mass="62371" checksum="54736886920620BB" modified="2022-05-25" version="1">MRDHGARCFGLIFGSGVACVLINNSCELPTERREETRPRAEAKRATRSNQHLATMVFLLDAEAERRAGQGGDPDPDPAAVTSREMDQTQRLPEDVLADVLRCLPPRNLAAARCVSRSWHAVVDAHRLLDRDLLPLSLAGFLINFYDFCFTEFFYPSSSTDTAIRGYGDLHFMPTDYIPVEDACNGLVLIDLDHYVVNPATRRWASLPPRPPLPLEFKGFHERGYIVFDPTVSPHYQVLRFLHVPPVYRLDAEVKQRQWPPSPYMMHVFSSETATWEERSFIRQGDALGTLADIGQLELPMSDELYAVYWHGEIYVHCHFVMRISLSSRTYQVIKLPTDVDQHRWTEVLLGRSEKGVYFAALDAQCQLRVWILTELPCDRSEWLLKHDNNLHQIPPHQNYDPPIHRPWIMADINYNFNPAIYSTVQDEIELNPDNEMADINYNFNPAIYSTVQDEIELNPDNEIILETRLERHTNEHIKILGFHPYKEVIYFSQSLDKGFAYHLHELKLEDLGRVRPKGLDYCYTKLVERSIPYTPCMFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM33</accession>
    <name>A0A8I6XM33_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XM33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0265340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0265340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0265340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0265340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0265340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0265340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF290">
        <property type="entry name" value="OS04G0422900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0265340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8582" checksum="87A45A80ABC986DA" modified="2022-05-25" version="1">MVACDFDLNFTFISCGWEGSATDARVLHSALNRGFKVPKGMFYLVDGGYANTTYFLAPYRGVRYHLKEFGHGCHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRT1</accession>
    <name>A0A8I6XRT1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123443079</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975265.1">
        <property type="nucleotide sequence ID" value="XM_045119330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRT1"/>
    <dbReference type="SMR" id="A0A8I6XRT1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0242950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0242950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0242950"/>
    </dbReference>
    <dbReference type="GeneID" id="123443079"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0201650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0201650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0201650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0242950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0242950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0242950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443079"/>
    <dbReference type="OrthoDB" id="536399at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF1">
        <property type="entry name" value="NF-X1-TYPE ZINC FINGER PROTEIN NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="854"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0242950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="899" mass="99102" checksum="F87448A26FF9AA33" modified="2022-05-25" version="1">MPSSYAAATASSSRKLAPSTSATAATRKPAPLPAPAPPPSNPSHVSDSDPSSYSSSGEETDLSACDPATASVLSTYLSVAGNGADLSKVGIFLNSAARRRSPPCLICLDPIRPSDPVWSCSASCFALLHLPCIQSWAHQSASAAPSPTWGCPKCRVAYPKSQTPTSYHCFCSKTEDPPADPWILPHSCGDVCGRRLNSNHDSGCEHTCLLLCHPGPCPPCPAVVPNARCFCGAHREPRRCAHQRYSCKGKCNKRLSCELHRCPVDCHDGPCPPCAVQGNHKCECGETMVERLCSERVFQCKRECSGMLECRKHRCERGCHGGKCGECPLRGRRTCPCGKKDYPRLECDVEAATCGSTCEKVLGCGRHKCPERCHRGPCDVTCRLVIKKSCRCGVLKKELPCHQDLTCERKCQRSRACGRHACKRRCCVGDCPPCAETCDRKLKCGNHKCLSPCHRGVCSPCPLMKTISCFCGKTYFEVPCGTEKNQKPPKCSKRCNIDRLCRHKLDCRPHKCHYGACPPCKLICGEEFSCGHKCKERCHGSIPPPNPEFTVKPMKKKMEKHIECTPGTPCPPCQEVVLVPCFGEHLGQERAIPCSKSRQFPCQNLCGNLLHCGNHYCTKDCHVLEIPSDQRKADAILSLSRKNTLAEPCERCNLPCQRARDPPCSHPCPLRCHLSDCLPCKALVKRSCHCGAMTHAFECVYYNNLNAKQQLKVRSCSGPCHRKLPNCPHLCSEICHPGQCPSVDQCMKKVNVRCACNTLKKEWVCQDVLKEYRRSGRDPKEVPKGQFGVGLLACGGDCLKKVNVSAAELHIRKVQETKNPAAEVANVPKRRKKRDRGAQEPVQVSMWQQIKRCLVVIIALAGLVVLGLVIWKGIYQISDWMNEMEEQKARERLLRAGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSM0</accession>
    <name>A0A8I6XSM0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123399661</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044949989.1">
        <property type="nucleotide sequence ID" value="XM_045094054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSM0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481900"/>
    </dbReference>
    <dbReference type="GeneID" id="123399661"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0399460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0399460.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0399460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0481900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481900.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399661"/>
    <dbReference type="OrthoDB" id="765963at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481900.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="172" mass="17539" checksum="2DFF5338AF084C0A" modified="2022-05-25" version="1">MASIMSMSSFPGAAVVLRGSACSFGARSLPALGRRALVVRAQTEGPSSPPPNKPKASTSIWDAMAFSGPAPERINGRLAMVGFVTALAMEAGRGDGLLSQLGSGTGQAWFAYTVAVLSMASLVPLLQGESAEGRAGAIMNANAELWNGRFAMLGLVALAATEIITGAPFINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTY4</accession>
    <name>A0A8I6XTY4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123396329</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947223.1">
        <property type="nucleotide sequence ID" value="XM_045091288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTY4"/>
    <dbReference type="SMR" id="A0A8I6XTY4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0438400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0438400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0438400"/>
    </dbReference>
    <dbReference type="GeneID" id="123396329"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0362970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0362970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0362970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0438400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0438400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0438400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396329"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF31">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="487"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="522"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="557"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="592"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="627"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="662"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="732"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="767"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="802"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="837"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="872"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="907"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0438400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="972" mass="105583" checksum="478E343B75A70E11" modified="2022-05-25" version="1">MGLTGTKRKLPFGPTPALTVTPAVEAPLPSPPMAAPPQPLQASFLCSLALALLRAGRLSAASHVASTLPAASPPAALLRRLIPALASSGLAAAAVRFRPVPGDPLSLNSILLSHCALRSLRPALALLRASESADTVSYNVVISGLTEQGRHGGLAPALLAEMCKRGVPFDAVTVNTALVALCRDGQVEGAAALAEMMVRGREIHRLDVVGWNALLDGYCKSGDMEAALTAAQRMRTQGVGVDVVGYNTLVAGLCRAGEADAARGMLETMKGDGVEPNVVTYTTFIAECCRTNAVDDAFSLYEEMVRMGVLPDVVTLSALVDGLCRAGRFSEAYALFREMEKIGAAPNHVTYCTLIDSLWKARRGSESHGLLGEVVSRGVVMDLVMYTSLMDWLGKQGKIDEVKDMFHCALSDNHTPNGVTYTVLIDALCRAGNVDGAEQMLLEMEDKSVHPNVVTFSSIINGLTKQGLLGKAADYMRKMKERGIDPNVVTYGTVMDGFFKCQEQESALDLYHEMLCEGVEVNKFIVDLLVNGLRKNGKMEEAEALFRDMNKRGMLLDHVNYTTLIDGLFKMGNMPAAFKVGQELTERNLSPDAVVYNVFVNCLCMLGKSKEAESILKEMQTTGLKPDQVTYNTMITAQCREGKTAKALKLLHEMKRSSIKPNLITYSTLIAGLFEVGSVEKAKFLLNEMASSGFSPTSLTHRKVLQACSQSGRPNMILEIHEWMVNAGLSADITVYNTLLRVLCYHGMTRKATVVLQEMSGRGIAPDTITFNALILGHFKSTHVDNAFATYDEMLRHGVSPNIATFNTLLGGLESAGRIGEADKVLNEMKRRGIEPSNLTYDILVTGHGKQSNKVEAMRLYCEMVAKGFLPKVSTYNALIGDFVKVGMMSQAKELLNEMNKRGVPPTSCTYDILVSGWAKLRNGTEVRKLLKDMKDKGFSPSKGTLSSICRAFSKPGMTWEARRLLKKLYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVV2</accession>
    <name>A0A8I6XVV2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Nuclear genome-encoded factor required for normal assembly of chloroplast polysomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVV2"/>
    <dbReference type="SMR" id="A0A8I6XVV2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0419240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0419240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0419240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0504770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010197">
        <property type="term" value="P:polar nucleus fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000009">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1910:FF:000003">
        <property type="entry name" value="Cysteine--tRNA ligase CPS1, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056411">
        <property type="entry name" value="CysS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23493">
        <property type="entry name" value="CysS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
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    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
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            <begin position="427"/>
            <end position="496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00005594"/>
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            <dbReference type="SMART" id="SM00840"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
    <sequence length="573" mass="64070" checksum="ECC9CC0D90EDAA46" modified="2022-05-25" version="1">MMAAAAAAAARRAATGLFPLLLSSQSRALPRHGVSLALPALVRPRRLPSHPAKLFFCSATASSNGAAPEKARDMHLYNTRSRRREPFRPRAPGGEVGMYVCGVTPYDDSHIGHARAYVAFDVLFRYLRYLDYEVRYVRNFTDIDDKIIARANQLGEDPFSLSKRYSDDFLSDMAHLQCLPPSVEPRVSDHIDQIVTMIKQIIDNGCAYVVSGDVYFSVDNFPEYGKLSGRKLDDNRAGERVAVDERKKNPADFALWKAAKDGEPWWDSPWGPGRPGWHIECSAMSAQYLGNSFDIHGGGEDLIFPHHENEIAQSRAACCDSSINYWVHNGFVNVNGQKMSKSLGNFITIRKVIELYHPLALRMFLLGTHYRSPINYTVEQLNVASDRLYYTYQTLRDSEESTQQDQSSSGDSLPFTTTHCIEKLHEDFETSMSDDLHTSVALAAVSEPLKVMNDLLHTRKGKKQEKRLESLAALEEKVRMVLSVLGLMPSSYHEALQQLRGKALRRACVTEEQVLQKIEERTAARKAKQFGRSDEIRDELAALGIALMDGPDGTAWRPSVPSSEQPVAGKSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XY10</accession>
    <name>A0A8I6XY10_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference evidence="3" key="1">
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <location>
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        </location>
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        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
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        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0349110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="191" mass="21025" checksum="0A414DC4076C69AA" modified="2022-05-25" version="1">MWHYTGPEDSTRTNVVGVPEERVTSWVLQITGPCENPKGARRVKPYSADHHPPNQAWTNWFSPVSNRSPEEEEEEGSQEGSVESAEYVFDSRESEEEDSHKEEEDEEQDSPPPRPEHRSKRRHEPAVPSAPPASSSAPPAAPAVPSVRGTKRARDAAAEPAGQSSRVPKPSGPKPRKALPRMRVAIPVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
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    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045095222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYK2"/>
    <dbReference type="SMR" id="A0A8I6XYK2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0550830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0550830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0550830"/>
    </dbReference>
    <dbReference type="GeneID" id="123401424"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0457720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0457720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0457720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0550830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0550830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0550830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401424"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724:SF39">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="AQUAPORIN NIP2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0550830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="279" mass="28741" checksum="AABFF68D4346744F" modified="2022-05-25" version="1">MDESRSGVVDISLNINPAFSMDSMRATPVMMPRRSPSVKIVPIASDDENEKSPSPGPKTAVVHLVKKVMAEFLGTFLLIFIVLSAVIMNATHGGALGLLGVAATVGAAGVVIVASLFHVSGSHLNPSVSLAMAVFGYLPWAHLAPYVSAQFLASVSASFVAKAIYHPAVNLGAVVTTVPAIGTLEAFSVEFIITFILLFALLAPATDPKAVKELVAVAAGAALMMNVLIAAESTGASMNPARTLGTAIATGTYTKIWVYMVAPPLGAIAGTGAYIALKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZJ7</accession>
    <name>A0A8I6XZJ7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0378040.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0378040"/>
    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0378040.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26519" checksum="DBE7297AFA27B4F2" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTYRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEILENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFLDPQVELLGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1Q2</accession>
    <name>A0A8I6Y1Q2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1Q2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292640.1">
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099:SF0">
        <property type="entry name" value="GERM CELL NUCLEAR ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
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    </dbReference>
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        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="285" mass="32971" checksum="4687D01A8D355B32" modified="2022-05-25" version="1">MGWDPFFRFSRSSFAYVDFQCPSHKALAKLFSTYKGCKWKGGKLKLEKAKEHYLVRLKREWEQEAATAATAAQEMPAKDDMDKQEEKEKRKLEKHALETSKVNIYFPRLRKPKPLPFKGSGKHKYSFRNIEIPSYPIHFCDCEEHCGPPEKANEEYAAVLNRVAFQKERNIMNYVMSKLFEKDTEQINSSEVQKGDAETDPSDAEAKPCDTETEPSDFDNDLRMEETTESPEEDLDDLQMEETDDPSEEELDDDVVINIAPRKANNSVVQLNRANQAMNKMQNNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1R1</accession>
    <name>A0A8I6Y1R1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC123444547</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977241.1">
        <property type="nucleotide sequence ID" value="XM_045121306.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977242.1">
        <property type="nucleotide sequence ID" value="XM_045121307.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977243.1">
        <property type="nucleotide sequence ID" value="XM_045121308.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977244.1">
        <property type="nucleotide sequence ID" value="XM_045121309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1R1"/>
    <dbReference type="SMR" id="A0A8I6Y1R1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292850"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292850.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292850.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292850"/>
    </dbReference>
    <dbReference type="GeneID" id="123444547"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0243550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0243550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0243550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0292850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0292850.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0292850.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0292850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444547"/>
    <dbReference type="OrthoDB" id="206335at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031125">
        <property type="term" value="P:rRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000019">
        <property type="entry name" value="RNA exonuclease NEF-sp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF157">
        <property type="entry name" value="SMALL RNA DEGRADING NUCLEASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="7">
        <location>
            <begin position="194"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0292850.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="548" mass="60446" checksum="5FC2F4B9C8DB0202" modified="2022-05-25" version="1">MSTPSDGAAPAASTYYDVFGPDAKPDVIFKEATSDSTLNLQDVQGLVTWVIGEGMPPSWVFVKNKPLITKVVLLYVPGLDAALYMSQSRLLSSLKELCGNPKPVLASSCIPDERHTIDALLTCRVKRKRQSQTSNQSNESDGGKLSSLADLKDIPFSVKYYTLSKKDLEDNGYPLSLPGFVPTVAAPSGSSPYEILGLDCEMCVTAAGFELTRVTLVDIKGEVILDKLVKPTNPITDYNTRFSGITAEMLSDVTTTLQEIQEEFVGLVYKETILVGHSLENDLLALRISHDLVIDTAVLYKYNRGPRCKIALRVLANKYLSRVIQNTGSGHDSVEDARAALDLAFLKIKYGPDFGSPPSFSRRKLASILHECGKRSSLIDEVFVLDRYSDASSNSIAVFSDDNALSRSMKEVKNDKISFVWTQFSGLISYLRKRAEDPVKLKSCVAEAIALKTCDRKTARKRAKQICPELKGILSELDKKIRKLHDTLPENAMFIICTGHGDTPLVQRLKKMLNHGEKTVDSRENIVHALEDLQAQAEVALCFCCVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2M3</accession>
    <name>A0A8I6Y2M3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="8">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123401582</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951346.1">
        <property type="nucleotide sequence ID" value="XM_045095411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2M3"/>
    <dbReference type="SMR" id="A0A8I6Y2M3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592920"/>
    </dbReference>
    <dbReference type="GeneID" id="123401582"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0491430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0491430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0491430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401582"/>
    <dbReference type="OrthoDB" id="2194683at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000599">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001558">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="10">
        <location>
            <begin position="216"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="437"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="519" mass="56927" checksum="2831EFE2A8A9B45E" modified="2022-05-25" version="1">MASQQPSLAILVREPDGFTVWPGPPYPPGSSPPQRLPKTACSATSFSTDGSRLLVTVAQASATVYDCSSLSVIRSFDLPGLTAAALSPTGSYLQTFQKSSSPQVKNVTVWHVDSAAALYQHYQKNLSKANWPMVQFSADESVACRMATNEMQFFDTNDFTKGIAYKVRLPGIAAMQLASAPGSHVAGFVPEAKGVPASVQIFSCDKDAQNQVVARRSFFRCSTVQFHWNKGSTGLLIVSQADVDKTNQSYYGETKLHYLTTDRAFEGIVPLKKEGPVHDVQWSSSGSEFAVVYGFMPAKATIFNKKCNPLHELGEGPYNTIRWNPKGRFIVIAGFGNLPGDMAFWDYTEKKLIQKAKAEWSVISEWSPDGRHFMTATTAPRLQIDNRIKIFDYTGSLQFKKDFEKLYQVDWKPESPERFGDIADLTISLNSIKIEETKKQGQGSKSAQTSSKAPVNVATKPAGAYRPPQSKHTAALQDKLFGGLAPTGGEMSKTALRNKKRREKQKEKKAGEGSSADDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4U4</accession>
    <name>A0A8I6Y4U4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y4U4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0490930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0490930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0490930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592290.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592290.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="121" mass="13638" checksum="962CD05291D6E53D" modified="2022-05-25" version="1">MEERSSPAISRFFTKSKTTQNYYFFPFLEVISSISMSVSCNSKLEVSLSNVHVCTWAAHVMSIILRRVRCTIRVPGPMGYAHVKGGTALFVGQGSRPCSHKPNQTKRQSTYSINFKVHHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAX1</accession>
    <name>A0A8I6YAX1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123412966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961852.1">
        <property type="nucleotide sequence ID" value="XM_045105917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAX1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0697480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0697480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0697480"/>
    </dbReference>
    <dbReference type="GeneID" id="123412966"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0578790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0578790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0578790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0697480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0697480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0697480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412966"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF9">
        <property type="entry name" value="LETM1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="578"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0697480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="922" mass="103104" checksum="19506C3BCC839021" modified="2022-05-25" version="1">MATAVASQPRHFTLGGEPRAAICAASTSKICTLDKAYGFSFVCRSVVDLRSKKFHSQISKRKCYLTSSPSEFEKIILSVRWLEFRRQRIAFQRTRRWVRSIPSASQDDGSGVSVNGVPQVDPASQMKEIRVKLDKALQNEDISSGLVQSIHDAARSIELAFLAHSKSSTNSWFPKTWLGIDNNAWIKSLSYQAAVSSLLQAVIDVSSRGNGRDRDINVFVQRSLSRLLSSLDGVIQNEMAKREPSLYQWYSSNQNPLVVRTFVNTFENDRRFNSATEICSEGKPMDTSESDLSLLVLGLFCLAAIMKLGSAKVSCQQFFSMVPDIIGRFMDMLLEFVPISKAYTLTKDIGLQREFLCNFGPRAAVPKFANDHGLEISFWIDLLQKQLLKALDREKIWSRLTTSETIEVLEKDLAIFGFFIALGRSTQVYLSSKSHTDSNDSISGIVRYLIGGSVLYYPQLSSISSYQLYVEVVCEELEWLPFYYEDVPTPIVDTEGRQETPKRELISRVLNVCTYWMTNFIKYSSWLENPSNVKAARFLSKGHAMLSDCMTELDIAKNNMPKDRSLQETGVLVDVTELASIDKSLESVEEALVKLENLLQELHLSSSNSGKEDLKAACSDLEMIRRLKKEAEFLEASFRAKAEYLEADTISSVLPPAGEGEHGKMGSMVNEVLTPQKPVNRMENNRRPFWDFFGTTSGRKIVPAQQALDQDASAAKVDQIDMESNDIRRFEQLRRELIELERRVQKSAGGAKKEEFQETEVQDETMSSFESAPPLSGPSGSVIKKENVITKSVEKVKESTTIVLQGTQLLAIDTGAAMGLLKRSLIGEELTQKEKQALQRTLTDLASVVPIGILMLLPVTAVGHAAILAFIQRYVPSMIPSTYGPERLDLLRQLEEVKEMKLSEGSSEDISDAIGLRGDQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YFN7</accession>
    <name>A0A8I6YFN7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123411915</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044960805.1">
        <property type="nucleotide sequence ID" value="XM_045104870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YFN7"/>
    <dbReference type="SMR" id="A0A8I6YFN7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726280"/>
    </dbReference>
    <dbReference type="GeneID" id="123411915"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0602360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0602360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0602360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411915"/>
    <dbReference type="OrthoDB" id="338531at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000018">
        <property type="entry name" value="AT-rich interactive domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF0">
        <property type="entry name" value="PROTEIN DEAD RINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="7">
        <location>
            <begin position="189"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="364"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="52569" checksum="B8DA1FFCD227A524" modified="2022-05-25" version="1">MDLNRTIWQFEGDDAPLVQVEHKANTTTDLPVGNSVGIEEGKSIMVGSGLGERVCSINCIQIPGNTKVIDVDTGGVDKKDDLMVHSQDKTSFVSLHNKGFTYSRRDKYTSTEVQKLENKDINSKLESEDGISLEERSNIFCDMKKDMNDENHLQPALVRKDEGQSALLKISNNSLMFGNTHSDEDSGTEEEQDNFFNELEKFHKDNSIEFRPPKFYGKGLNYLKLWRKVKKLGGYSKVSSKRMWRQVGESFKPPKTCTTVSWSFRNFYEKVLLKYEKPNNETCRFKDIPSTLTKQNASRKEMSETSVLGRVKRESATLAMQRWSPQKSMRNVSQKRKTVPTIEDDKDVQRGVDNTQSKPTIIDMGSRADWVKINVFITKYHFQVYALVPGLLHEELLVLSDPIGRLIINGDPRQLDNPWGLTRFHKVIQLPSRINPEKTLTKLSEYGKLYVCGEFEKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YIW3</accession>
    <name>A0A8I6YIW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YIW3"/>
    <dbReference type="SMR" id="A0A8I6YIW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="323"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="360" mass="37893" checksum="E95D4BEB431F3B3A" modified="2022-05-25" version="1">MPRGKIAMRLVENARARAATCGRRTRGLQNKAKELATLCAVPVALVCLAAGAGSPPLVWESEEGVLERYRSAVPPEARAQHTHRGYLEAELGKERAKLARVRHGCPAALADWDPALNDVTLAEARELLDAIDTALRAAGDRMEALGLPADGALDEQVAPDASDDAVMPQPLAHGGGVPCTGSNPVDMDVAGFQLQMVPWNGGDKDGLLGEDGFQMQPGCGFECIGGGNFWGAVDGTLAPGSGNAHYDWPGLTMWHADELCDAALPLGHYPNFADGTLAPEYSGQVVAGGNYMKTLPSGGYPMAMGVGDNFTLGSNYTAHWQAEESHRSGTSTSQLLSAASSPQCLNPGTRSSSQVFHYLR</sequence>
</entry>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0514910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="142" mass="16151" checksum="ED55F927748060DE" modified="2022-05-25" version="1">MDIALLEVLVEHHNNGDHAQNGWKSHVYVAVITNVREKCNVTITKENIILRCKTFEKHYEAISKMRSQSGFGWDWVNNKLSIDSEDVWTKYVVANNKIAFYKNRVIKNWDAITTIYSNDHANGEGVGTSIESVVEPTVGNMP</sequence>
</entry>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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                <person name="Muehlbauer G.J."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0673700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="121" mass="13326" checksum="DC22327CF73EDE07" modified="2022-05-25" version="1">MHDGAAGNTTTTHRLDRRIKGRCARRMSRLSPGFGSWVAVGLTRKPPPPVPLLPRCSSTRHARRLTSELLGLFDTFFASLSMILVSEVSCHFSDTNATTTVFLNLLSCLRSCSVILQLPKI</sequence>
</entry>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">cv. Morex</strain>
        </source>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0680490"/>
    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <location>
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            <end position="216"/>
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    </feature>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="25049" checksum="5960F69CDF4F23C0" modified="2022-05-25" version="1">MGREDRFPLWEAALGAGVAAVFAAVLVGVYLSMLDSDYSFLKLPRNLHELQILTGHLENYTSDYTVQVLIGYCDVYIFMQTFMIPGTIFMSLLVGALFGQLRGVALVVFAASAGASSCYFLSKLIGKPLVFMLWPDKLTVTPTLPNTFINLASPMVDVLFHTFLLATLIGLIPAAYVTVRAGIALGELTSLSDLYDTQSVALLFLIGVVSVTPALLSKDKAQEKAPEIAAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="3">DUF1499 domain-containing protein</fullName>
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    <reference evidence="2" key="1">
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
            <begin position="48"/>
            <end position="194"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <sequence length="216" mass="25197" checksum="4B3F2054A5CCE4A1" modified="2022-05-25" version="1">MPTRFEEVLAHGTTMLDVVYTNESREMPFFLEQLKERWLDAAMDHEKFLGLDLEYTADQRGVAVIQLCFAHHVLIFQWASSDKHCPELMDFLRSGITFATVDIRNDKLKMRYNFGIEIPIGCLIDLQTVFRLRHVRTSMAHTAVALIDEEYGDMKTSFPKSQHKLWEKAPLDRINIEYATKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
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        <lineage>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
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    <reference evidence="7" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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        </subcellularLocation>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="194" mass="22398" checksum="AA3662567902B765" modified="2022-05-25" version="1">MELFTIILEKSSSRQVLPDNFVKMLDGHRPQNMKLRQAVNGLRKLWDVEVVFDTDGSMYLDRGWKQFVRAYDLRDGYFLVFRYDGNATFVVKVFDTTMCRRHYQDDDDASNGSRSSEYCDRCYVYGSSDSGYSKSSSDSGYKKSSSDDGLAMVIPQLGDRAMPILVEEYIPQPGLGLRRSTRIRMMKEKVKKQE</sequence>
</entry>
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    <accession>A0A8I6Z2Y1</accession>
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        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
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    </organism>
    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0696090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="91" mass="10556" checksum="BF439ACB8740FD52" modified="2022-05-25" version="1">MDVKTAFLNGELDEEIYMEQPDGFVVDGHEGKVCKLLKSLYELKQAPKQWHEKFERTLTTAGFVVNEVDKCVYYRHGGARELSFVCMLMTY</sequence>
</entry>
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    <accession>A0A8I6Z5I9</accession>
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        <recommendedName>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5I9"/>
    <dbReference type="SMR" id="A0A8I6Z5I9"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635240"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0527780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0527780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0527780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0635240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0635240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0635240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
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        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
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    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="89"/>
        </location>
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    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="9">
        <location>
            <begin position="92"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0635240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33248" checksum="6BA148C4B8DB4B81" modified="2022-05-25" version="1">MSGTTTMHWEGTKHRPTHGRHWTQRQMWGKFFASTTFYDPTKRRRVMWAYVGETDSNRTDIAKGWANLQAIPRTVALDEKTRTNLLQWPVEEIDTLRHNTTDLSGITIGTGSVFPLHLRQAAQLDIEVSFRLNISDIAVHNEADVGYNCSTSGGAANRGALGPFGLLLVTTNGHSEKMAMYFYVSRSLDGGLRTHFCHDESRSSLARNIVKRVVGSTVPVLDGEAFSARILVDHSIVESFVMGRRLTATSRVYPAEAIYEAAGVYVFNNATGSTVTVEKLVVHEMHSTPMQLDLYVRD</sequence>
</entry>
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    <accession>A0A8I6Z7B7</accession>
    <name>A0A8I6Z7B7_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">DUF569 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC123412292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0654810.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0654810.1">
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    </dbReference>
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        </location>
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        <location>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0654810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32362" checksum="34DC19B570B1A9E5" modified="2022-05-25" version="1">MDQFHDRQHVRLRSRVLGTYLHADHDGERVSLSRRRDSLHAAWAVHIHPRPDGPYLLLHSAAYGRYLGATFKPSPLGHLGCRTEQRDYDDEQDLMAVMWRAAGAGFRDVVLLRSVSGRFLRANGRYLPWNNGVTVDDNVSAMMYWTVEPIPARGGPPGLPGPIHTPSPGYHSLMLWRNPVVSWLIRFMPSDPNGPIYSRYCWTTFRYRGRSVFHLRDELARRMAFVLDGRQSFDLVMCVRAGREGRLTPLVVDLPSSGYGETLSIVVIMSGTPAYNALRHPDVDAA</sequence>
</entry>
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    <accession>A0A8I6Z7X3</accession>
    <name>A0A8I6Z7X3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123410622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045103565.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751430.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0751430.1">
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123410622"/>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR31210:SF50">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210">
        <property type="entry name" value="OS06G0731900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05212">
        <property type="entry name" value="DUF707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0751430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="388" mass="43460" checksum="31F87CB81BE08F42" modified="2022-05-25" version="1">MKMKASPVLTVLFAAAAGFLVGISFPVDITPKLQCGILPWSSNSGGVANCSVGHSSRLGRLWGAPFRNNSTGAPNATSSAPIVAVAAVRNPREGAERRLPPGIVVSESDLHLRRLWGSPREDSPTGKYVLALAVGYGERENVNATVHKFSDNFDIVLFHYDGHTTEWDEQFEWSKEVAHVSARKQTKWWFAKRFLHPSMVAPYDYVFLWDEDLGVDNFAAEPYLDIVRRHGLEISQPGLDTTEGPAPMYDVTTRKNSSEMHKTDAGGEHCWDVHKRPCSGFVEMMAPVFSRDAWRCVWHMIQNDLVHGWPIDSYFWRCVDDPEEQIGVVDAQYIVHRAVPTLQGEGDKGKPGGRFEVRARQFEELEAFNSRFANADKELTNRSLSTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZFC1</accession>
    <name>A0A8I6ZFC1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="990"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1054"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1206"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1227"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0095330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1415" mass="155842" checksum="2C7B04EE7B3C1B41" modified="2022-05-25" version="1">MIGDDAHPTTPPPSPPPIAPTPSPLGLGRRLLGSIRALSSAIALPSSPTTPAAASSSPRLGRGLSLLLHLKPDLQLNPPPPSSTASASASASASHQDQDQDQDAEDQQPTHAPKAKRALILAPQTTTPSLAPSAPVHHHHRQDETQGGHDPGPTKGLNVLHQHKGDLPLLTHRHQPTLFSTVHHRLAAAAPMDDRHPHPHRDTSLVHHHHLKVADAPIHDDDHHLKLLDATAPPTPRDHAIAAAAAHQQQELPTHGLPTGSTRDNRAVQPQEVQLEQGVVALAGVGLATQDDTAVDRDATIHDHNYAIAVDAHHQEQVKPLLEQCTSTSTSCEDLGTPAMDNDDDAVQLQEVMEQQHATHPRDEYIAAEEQSEMLDRELGSTEKADAVPVQEAAEQGLVGTDGESADATAVDGHDMAVEQCVTCHDLGTTTENDGAKDDQEQGVIDQGDAAIDGDNDVKHHKVLVPGAVEGTHTSSDDIAVEDQGKNVEQCTTYDVLGTTMKNDDAIAVDDQQVVEQGVVDLTTIDDDPSVEEQEVVEQGVVDLTTMDDDPSVEEQEVVEQGVVDLTTMDDDPSVEDHEVLQQGIIGGTVDIAVQKQEKPLEQCSRATKDEYTAKEKEMVVEQGVIDPKYMDIAMEDQDKLLIIDDQDTVPMDDIVVKDHDKAVVEYASDAIVSIKDESPVEEHDTVGSQGVIDKNGRTKDDIDVEGHGYVKELVIIDKWGTSSDAAALEGQKNETEPCIGDEQIAGKDMDGVHAEANMLEQMTGDKQGATLSDFAVDKHKDVVERVRHEWGAPENDLAMDTAAYQGTRDWGIVNKEKYRAPARRYPQKPRKLNCPSYMSKGTCTYGPSCHFNHPPQFNAKTNDSWRPSERRDHGAAEILELNRLGLPIREGARNCDYYMRTGACRYGKNCHFNHPDHVIDARFSSPTGWEDNALETEKSSDHALTEASHMKKASDDATLNDRSHLKKSSDGATLDDRSHLNKSSDNATLDDRSHLKRSSDGATVDDTSYSKKSSDHDTSSTSGVLPPSIFRMLLPPQKVLPSTEGKAKKKSDWSSASDDSDGCCSADSSDGPLCKQEEHVDYPERPGRQECPFLARYGDCKFASACKYHHPKQSKDKEQVNYPERPGRPDCPFYMRFGDCKFASACNYHHPKDKYPTGLPEEPECPFYMKRGFCKFGAQCKFYHPEDANPTMQSPTDAKISVTMDKHHPSTRITPEDHVPQQPQYPERRSVTTDDHHPSIRITPEVLPQQPQYPERPGQPDCRYYMQFGKCKYLSACIFHHPKDRLAAMWSPSDPAHSDQIGPKIHGMPDCPFYMKSGKCQFGSLCEFRHPKDIYSTTEEAFGERTGSGAYDSLTRSDNGVEQQEGSVMYPERPGEPECAHYMRQGYCKFQMNCKYHHPGDRLSKKPYYDSTCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B215</accession>
    <name>A0A8I7B215_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123424516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044964080.1">
        <property type="nucleotide sequence ID" value="XM_045108145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B215"/>
    <dbReference type="SMR" id="A0A8I7B215"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0104670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0104670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0104670"/>
    </dbReference>
    <dbReference type="GeneID" id="123424516"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0085950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0085950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0085950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0104670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0104670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0104670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424516"/>
    <dbReference type="OrthoDB" id="37297at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd02972">
        <property type="entry name" value="DsbA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF5">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035192750" description="Thioredoxin-like fold domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0104670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25197" checksum="556DAF54FF1E4902" modified="2022-05-25" version="1">MEARRERHRVSPLLLVLVAVVTAAGRCGAQVPVPARTVGFVYGGQAWAPAWGEAVVVEAFFDPLCPDSRDAWPPLQRAADHFGARRVAVVVHLFPLPYHSNAFIACRSIHAAHKLNASAVYPLLEKFFKYQEDYYNAPTYIKTRAAVVAEIANNLVTPIIGEANLAAYIAGFNDSRSDQAARISFKFGCARGVTGTPYYFVNGIPLNDLDFPLNYDKWISTLDPLVGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4A4</accession>
    <name>A0A8I7B4A4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4A4"/>
    <dbReference type="SMR" id="A0A8I7B4A4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0212560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0212560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0212560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0212560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0212560"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF129">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE GALT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0212560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="227" mass="26806" checksum="091873A68A39DD63" modified="2022-05-25" version="1">MMVRRTWMQYDAVCSGKVAVRFFVGLHKNEVVNEELWNEGRTYGDIQLMPFVDYYSLILWKTIAICIYGTNVLSAKYVMKTDDDAFVRVDEILLSLRQVNISHGLLYGRINSDSQPHRDPYNKWYITSEEWPEESYSPWAHGLGYIVSQDIAKEIYRRHKRGELKVFKLEDVAMGIWINEMKKEGFDLTYQNDGRILVEGCEDGYVVAHYQEPRQMMCLWDKFQKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4B5</accession>
    <name>A0A8I7B4B5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123421191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    </reference>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </evidence>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <source>
            <strain evidence="3">subsp. vulgare</strain>
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        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0118980.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
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        </source>
    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
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        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
    <dbReference type="Pfam" id="PF05033">
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        </source>
    </evidence>
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        </source>
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        </source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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            <fullName evidence="5">VQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6W0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0344650.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0344650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0287710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0287710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0287710"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0344650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0344650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR039611">
        <property type="entry name" value="VQ_4/11/13/19/31/33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33402">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN 11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33402:SF22">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="5">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0344650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="106" mass="11858" checksum="C9DCB7854C50DA97" modified="2022-05-25" version="1">MDRNPRTPAPRVYTEVPPDTTVVRADASNFKEVVQRLTGQPAAANTVVHRHGATAEDVEMVDAEEEKAIKEGRFYLHPSLRSVRCICCHREPKLLPLFPVTPSHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC123430777</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044970551.1">
        <property type="nucleotide sequence ID" value="XM_045114616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B827"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194500"/>
    </dbReference>
    <dbReference type="GeneID" id="123430777"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0161360.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0161360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194500.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430777"/>
    <dbReference type="OrthoDB" id="49610at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
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    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF202">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="67"/>
        </location>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
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            <end position="171"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194500.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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    <name>A0A8I7B904_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
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        </recommendedName>
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        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
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    <dbReference type="RefSeq" id="XP_044946782.1">
        <property type="nucleotide sequence ID" value="XM_045090847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B904"/>
    <dbReference type="SMR" id="A0A8I7B904"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478260"/>
    </dbReference>
    <dbReference type="GeneID" id="123395812"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0396280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0396280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0478260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395812"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000382">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="103"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="544"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="679" mass="74059" checksum="DE064F9391B3E1BA" modified="2022-05-25" version="1">MRRDAAGGPGGGAGFHDLFDSVRRSINFRTSAAAPPEPPAGPLGGGPAGGIGVRISSCLRKSRGMGLLGLISKSPSPPRRLLPPTPVPADGGGRAGEIPAIRWRKGEMIGSGAFGQVYLGMNLDTGELLAVKQVLIGSTNATREKAQAHIRELEEEVKLLKNLSHPNIVRYLGTVREEGTLNILLEFVPGGSIQSLLGKLGSFPEAVIRKYTRQILQGLEYLHSNAIIHRDIKGANILVDNKGCIKLADFGASKQVAKLATMTAAKTMKGTPHWMAPEVIVGSGHTFSADIWSVGCTVIEMATGKPPWSQQYQEVALLFHVGTTKSHPPIPEHISPEAKDFLLKCLQKEPELRSSASDLLKHPFVTGEFDDRQLLNRTAQKDASVNELFTHDADAPTEMGLNHSGNWSTINSNRSSKIKPLWEGGGDDDDMCEFADKDDHRAVGSSYNPMSEPFDDWKSKYDISPEQSSHQSREFGGLAKHAESSMTENDFTFPCEGSCEDDDVLTESKIEAFLDEKALDLKKLQTPLYEEFYNKVNAGTSHGVDQTSNGKFINSPKLPPRGKSPPGKMRGGSAVATPCDTMFNSSTMAESCSKQFSRDGVDSSRILREIASPQLNELGDKVHVDVQDSPSISFAERQRKWKEELDQELERERVMRLAGCGKTPSPSRRPSTGKRERHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B9H5</accession>
    <name>A0A8I7B9H5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC123439658</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="20"/>
    <dbReference type="RefSeq" id="XP_044972285.1">
        <property type="nucleotide sequence ID" value="XM_045116350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B9H5"/>
    <dbReference type="SMR" id="A0A8I7B9H5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247450"/>
    </dbReference>
    <dbReference type="GeneID" id="123439658"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0247450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000384">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000370">
        <property type="entry name" value="Receptor-like protein kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000009">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
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    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8" id="KW-0732">Signal</keyword>
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    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="71"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="365"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="513"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="541"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="742" mass="80705" checksum="4789063F16D63B36" modified="2022-05-25" version="1">MAAATGVHRRHWSGWRIGGSSHFVPLKVMHTSGGCAARSLSPGLSPAKRAPLVFLFLLLIDGALLAASATTDTILPGEGISGNETLVSKTGSFELGFFSPGPGIHYFLGVRLRNMGHSPTFWLGNRVVITDLPGASLEIFGGSLYIKQNGASLWWTPSPGGNVSSAAVAVLLDNGNLVVRDQRNSSLVLWQSFDYPGDALLPGARLGLDKDTGKNVSLTFKSFSHNGSLSVDVTRRNGFVLTTDGHANLGTFPDWMVSSQDNGSSLRLSHREGPNSTEFLQFHLGQVSLMRYSEPDPDANGTGGWAARWSFPPDCKSGGFFCGDFGACTSSGKCGCVDGFTPSFPIEWGLGYFVTGCSRSVPLSCESDGQTEHGDTFAPLDKLQGLPYNAQGEMAGTDEVCRAACRSKCYCIAYSYGHGCKLWYHKLYNLSLASRPPYSKIYLRLGTKLRNKNGLQTRGIALLVTGLICFASLILIISVLLWRFRRNSFAARKFEVEGPLVAYTYAQIKKATMNFSDKIGQGGFGSVFRGTLPGSTDIAVKNLKVLGEAEKQFRTEVQTVGAIQHNKLVRLLGFCVKGDRRLLVYEYMPNGSLDTHLFPEKSGPLSWNVRYQIALGIAKGLAYLHEECKDCIIHCDIKPENILLDAEFCPKIADFGMAKLLGREFNSALTTMRGTMGYLAPEWLSGLPITKKADVYSFGIVLFEIISGRRSTKMMQFGSHRYFPLYAAAQGRLLVHPGSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAB4</accession>
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        <name type="common">Domesticated barley</name>
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        <lineage>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="107" mass="11582" checksum="EE235549CE3AB23A" modified="2022-05-25" version="1">MLQHVDPVRRPTESCSVTIPSSAVDQAVFFLRSHAVTLSATDGVNASSPVVVGRALEAQLSVPVHSLRVTTHHPEHFFVIFTQPTHQVNAVRRGSMRVDGAVFNIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0361240.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="124" mass="13942" checksum="9D36D0CEE42A900C" modified="2022-05-25" version="1">MEEMKDAPVTEGEEPKFDAEIVEEVLKTEVNRSTFLRNVGLKSSSNNSGKGTHVVAAHVRDLQQKLDRSELQAEVMQEEMAAIKLKAEEYEAACDKELELPRKKSREQDEKLAHLMALFGAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="11">GDSL esterase/lipase</fullName>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">cv. Morex</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979059.1">
        <property type="nucleotide sequence ID" value="XM_045123124.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8I7BCD9"/>
    <dbReference type="SMR" id="A0A8I7BCD9"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0381830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0381830"/>
    </dbReference>
    <dbReference type="GeneID" id="123446552"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0317620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0317620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0381830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0381830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0381830"/>
    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF4">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035151817" description="GDSL esterase/lipase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0381830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="38941" checksum="793A5DEB712CEF02" modified="2022-05-25" version="1">MSSRLTLLACIGALFAGAAAAVPPATYVFGDSLVDAGNNNYIATLSRANYAPNGIDFDGHQPTGRYTNGRTIVDILGQEIGLGGFVPPYLDPNTTGDVLFRGVNYASGGGGILNQTGSIFGGRINLDAQIDNYAKNRRDMMTQRGEVAAVSRLRGALFSVTMGSNDFINNYLVPILSVPERAATPPEAFINGMIAKYRQQLIRLYLLDARKIVVVNVGPIGCIPYLRDIMTTGAGAGAGACAEFPNQLAQSFNRKLRGVVSELGAGLGGSRFVYADVYRIVSDIIANYKSNGFEVADSACCFVGGRFGGLAPCGPTSSYCADRSKYVFWDAYHPSDAANALIASRILDGDPADISPVNVRQLLFDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCJ8</accession>
    <name>A0A8I7BCJ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0386310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="91" mass="10449" checksum="6050305D85C0E862" modified="2022-05-25" version="1">MDVKTAFLNGELDEEIYMEQPDGFVVDGQEGKVCKLLKSLYGLKQAPKQWHEKFERTLTTSGFVVNEADKCLYYRHGGARELSFACMLMTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCR2</accession>
    <name>A0A8I7BCR2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCR2"/>
    <dbReference type="SMR" id="A0A8I7BCR2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0628820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="434" mass="49283" checksum="FA3AFBBF3DEC824E" modified="2022-05-25" version="1">MIRSTLKVAPSKGAMSRSMPLANAGEQGHPRTRIDNPKIGPEENVVMVNFPVASTQELRAPPPPRPASPVDIVIRDDMPLRLPGPNTSANVVLTQRPPSEMSAGGSEAVSALDHREVRKSELKHYLNDAQRDALEYLKEKVSEMQLGVIRMTDAFAPGIEAAARDNRRLEKEIARLKILLDEVEQKNENLSKSLGDSMVDKRRMTEMRKELNSNNQQLEDIQKEAQKIATKLVQEQKACQAACDASRLLQQELGKSQAECDQLKAEKTKLSDQIKFLKYSLKDSNLVRRNAQDEVEQANRIVDGQPYLLQCRFGNNSYATLTQIWHHAEIFADLPRSIVSAHKYYEELNDPEREVLQAFWAQFQETRRLDLLPDKLKQIGELLNLIKPTIEAIFMTLWPEEDIPTNFFDLAIRLQHAPPRILEWKSSKVREGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BI92</accession>
    <name>A0A8I7BI92_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BI92"/>
    <dbReference type="SMR" id="A0A8I7BI92"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="150"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="28031" checksum="644614DC62F31DDD" modified="2022-05-25" version="1">MVGVPHHQGSVNLIQFGENWARHNKTEAPQMYDLYAMAHTASYKKVKAFFESELEHPENFSNISSHHKLVKYRDHGKARKGEDFNPSEGPIDPELVMIAGNGRPHGSIAIGDGLIRCPSTLRQINARQTSSCPEITRRPRPVELAIEAAIQKEREAMQAAMAEKDKEIRELEERTTALVEAERAQNDASNRAIDELFVSMCEKNGQVPPSMPVINVAGTNISRNASHDPSRVEPSPGQPSRGETSQSHRASPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BK08</accession>
    <name>A0A8I7BK08_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BK08"/>
    <dbReference type="SMR" id="A0A8I7BK08"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0741140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0741140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0741140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0741140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0741140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0741140"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF11">
        <property type="entry name" value="DUF4201 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0741140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="197" mass="22730" checksum="99CF5CEFA6561ED7" modified="2022-05-25" version="1">MLEDNRSGRRDDNLENSLKIHHLIEEKRNLDANYDKLVEDVNQLLSTQQERVMDLSYMKVNAHGAESSSSDVSVMQSEIDKKEAEIMEVKGKYSVLMNLAESQGRVIRTQKANHLKEKEKLSEENYNLKVQVDKLTKSEDKLNDDIVVLNLHIDGLKKGIQNLIKRRDELKLEIADQLKTLEKNEEKLKMISDILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>F2CRE3</accession>
    <name>F2CRE3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123441704</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK354195">
        <property type="protein sequence ID" value="BAJ85414.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973926.1">
        <property type="nucleotide sequence ID" value="XM_045117991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CRE3"/>
    <dbReference type="SMR" id="F2CRE3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063350"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063350.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063350.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063350"/>
    </dbReference>
    <dbReference type="GeneID" id="123441704"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0050410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0050410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0050410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063350.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063350.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441704"/>
    <dbReference type="eggNOG" id="KOG0209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001828_4_1_1"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CRE3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000027">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000027">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000109">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="947"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="978"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1008"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1058"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1089"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1129"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="15">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="255"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="831"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="865"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ85414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1174" mass="131324" checksum="85B8DC414DA7FC1E" modified="2011-05-31" version="1">MARFEVNGKSVEGVDLLRRRHWTARLDFWPFLALYALWLLLAVPALDFTDALVILGVLSASHILAFLFTAWSVDFRAFVGHSKVKDIHAADACKVIPAKFLGSKEIVPLHIQKTVASSSAAGETEEIYFDFRKQRFFYSAEKDNFFKLRYPTKDLFGHYIKGTGYGTEAKINTAMDKWGRNIFEYPQPTFQKLMKEQCMEPFFVFQVFCVGLWCLDEYWYYSLFTLFMLFLFESTMAKNRLKTLTELRRVKVDNQIVLTYRCGKWVKISGTELLPGDIVSIGRSPSGEDRSVPADMLLLSGSAIVNEAILTGESTPQWKVSVAGRGPDEMLSIKRDKNHILFGGTKILQHTPDKSVNLRAPDGGCVAFVLRTGFETSQGKLMRTILFSTERVTANSKESGLFILFLLFFAIIASGYVLMKGLEDPTRSRYKLFLSCSLILTSVIPPELPMELSIAVNTSLIALVRRGIFCTEPFRIPFAGKVDICCFDKTGTLTSDDMEFQGVVSLESDAELISDANKLPLRIQEVLSSCHALVFVDNKLVGDPLEKAAIKGIDWIYTSDEKAMSRRPGGQPVQIVHRHHFASHLKRMSVIVRIQEKFYAFIKGAPETIQERLVDLPAAYVETYKKYTRQGSRVLSLAYKLLPEMPVSEARSLERDQVESDLIFAGFAVFNCPIRSDSAAVLLELEQSSHDLVMITGDQALTACHVASQVNICLKPVLILTRMKTGGFEWVSPDETDRVPYRAEEVKELSESHDLCVSGDCFEMLQRTDAVVQVIPHVKVFARVAPEQKELVLTTFKTVGRMTLMCGDGTNDVGALKQAHVGIALLNAEPVQKAGSKSQSSKLESKSGKLKKPKPATESSSQLVPPATSSAKAPSSRPLTAAEKQREKLQKMLDEMNDESDGRSAPIVKLGDASMASPFTAKHASVAPTLDIIRQGRSTLVTTLQMFKILGLNCLATAYVLSVMYLDGVKLGDVQATISGVFTAAFFLFISHARPLQALSAERPHPNIFCAYVFLSILGQFAMHLFFLMSAVNLASKYMPEECIEPDSEFHPNLVNTVSYMVNMMIQVATFAVNYMGHPFNQSISENKPFKYALYSAVVFFTVITSDMFRDLNDYMKLEPLPEGMRGKLLLWAMLMFCGCYGWERFLRWAFPGKMPAWEKRQKQAVANLDKKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTQ0</accession>
    <name>F2CTQ0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123453013</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK355002">
        <property type="protein sequence ID" value="BAJ86221.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985703.1">
        <property type="nucleotide sequence ID" value="XM_045129768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CTQ0"/>
    <dbReference type="SMR" id="F2CTQ0"/>
    <dbReference type="STRING" id="112509.F2CTQ0"/>
    <dbReference type="PaxDb" id="4513-MLOC_43575.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525620"/>
    </dbReference>
    <dbReference type="GeneID" id="123453013"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0437030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0437030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0437030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0525620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0525620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0525620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123453013"/>
    <dbReference type="eggNOG" id="ENOG502SIQB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="SGFMTRQ"/>
    <dbReference type="OrthoDB" id="207175at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CTQ0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080050">
        <property type="term" value="P:regulation of seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF67">
        <property type="entry name" value="OS03G0818800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="115"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="207"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ86221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0525620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="440" mass="48350" checksum="2FD3CA6692AC050F" modified="2011-05-31" version="1">MVLDLNVESPADSGTSSSSVLNSADAAGAFRFGLLGSLDDDDCSGELAPAAASGFVTRQLFPAPPPAPGVMMGQAPAPPPTAPVWQPRRAEELVVAQRVAPKKKTRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGLEADINFNLSDYEEDLKQMRNWTKEEFVHILRRQSTGFARGSSKYRGVTLHKCGRWEARMGQLLGKKYIYLGLFDSEVEAARAYDRAAIRFNGRDAVTNFDSSSYNGDATPDVENEAIVDADALDLDLRMSQPTAHDPKRDNIIAGLQLTFDSPESSTTMVSSQPMSSSSQWPVHQHGTAVPPQQHQRLYPSACHGFYPNVQVQVQERPLEPRPPEPSSFPGWGWHAQAVPPGSSHSLLLYAAASSGFSTAAGANPAPPPSYPDHHHRFYFPRPPDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2CTW8</accession>
    <name>F2CTW8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123413700</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK355070">
        <property type="protein sequence ID" value="BAJ86289.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="term" value="P:pyruvate import into mitochondria"/>
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        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
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    </dbReference>
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        <location>
            <begin position="44"/>
            <end position="64"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ86289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0016030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="107" mass="12230" checksum="8E50E5D461D03B33" modified="2011-05-31" version="1">MATAFKAFINSPVGPKTTHFWGPVSNWGFILAGMADMNKPPELISGRMTAVMCVYSGLFMRFAWVVRPRNYFLMATHASNESVQLYQLSRYARVQGYLEKKEDKDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2CWB3</accession>
    <name>F2CWB3_HORVV</name>
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        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123442350</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK355916">
        <property type="protein sequence ID" value="BAJ87134.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974296.1">
        <property type="nucleotide sequence ID" value="XM_045118361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CWB3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218450"/>
    </dbReference>
    <dbReference type="GeneID" id="123442350"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0181710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0181710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0181710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442350"/>
    <dbReference type="HOGENOM" id="CLU_074889_1_1_1"/>
    <dbReference type="OMA" id="GWYVVCY"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
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    <dbReference type="ExpressionAtlas" id="F2CWB3">
        <property type="expression patterns" value="baseline and differential"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AtER_Rer1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ87134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="198" mass="21843" checksum="97724EBD4717772D" modified="2011-05-31" version="1">MDSGGGGGGGGGAAAAVAKWRTDGTRAFQYYLDRSTPHSTGRWLGTLVVAAVYALRVLSIHGFYIVSYGLGIYLLNLLIGFLSPMVDPELDPSAANDGPALPTRGSDEFKPFIRRLPEFKFWYAITKAFVIAFVMTFFSVFDVPVFWPILLCYWIVLFVLTMKRQILHMVKYKYVPFNIGKQKYGGKKGGASSSSSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F2DAT6</accession>
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        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123411647</name>
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    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="AK361000">
        <property type="protein sequence ID" value="BAJ92207.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960577.1">
        <property type="nucleotide sequence ID" value="XM_045104642.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960644.1">
        <property type="nucleotide sequence ID" value="XM_045104709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DAT6"/>
    <dbReference type="SMR" id="F2DAT6"/>
    <dbReference type="STRING" id="112509.F2DAT6"/>
    <dbReference type="PaxDb" id="4513-MLOC_2793.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015170"/>
    </dbReference>
    <dbReference type="GeneID" id="123411647"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0011610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0011610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0011610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0015170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0015170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0015170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411647"/>
    <dbReference type="eggNOG" id="KOG0076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_7_2_1"/>
    <dbReference type="OMA" id="NIERCVH"/>
    <dbReference type="OrthoDB" id="414781at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DAT6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000914">
        <property type="entry name" value="ADP-ribosylation factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF2">
        <property type="entry name" value="OS07G0620400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ92207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0015170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="203" mass="23045" checksum="CEDB24DB5D26C3C6" modified="2011-05-31" version="1">MFSLFHGLWNHVFSKTEFRVLILGVHKSGKTTLLEKLKSIYLKGEGLPHDHIGPTVGLNIGRIEDANVKLVFWDLGGQPGLRTIWEKYYDEAHAIVYVIDSASASTFEDAKSALEKVLRHEDLQEAPLLVFANKQDLPAAVTEEELDRHLHLKEFDERPYMFVAGSAYDGTGIKLGIDWLVEEMGKSRRTEALRTRTDTYAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>F2DBM1</accession>
    <name>F2DBM1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425305</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK361285">
        <property type="protein sequence ID" value="BAJ92492.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964923.1">
        <property type="nucleotide sequence ID" value="XM_045108988.1"/>
    </dbReference>
    <dbReference type="SMR" id="F2DBM1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0125030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0125030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0125030"/>
    </dbReference>
    <dbReference type="GeneID" id="123425305"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0103060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0103060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0103060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0125030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0125030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0125030"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425305"/>
    <dbReference type="OrthoDB" id="767933at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008:SF15">
        <property type="entry name" value="GPI-ANCHORED ADHESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="911"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="991"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ92492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0125030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1350" mass="147709" checksum="0501C505F719ED88" modified="2011-05-31" version="1">MEPDAPLDYALFQLSPRRSRCELVVSGSGRTEKIASGSVKPFVTHLRTAEEQAAAQPPQPAIRLQLDRRAAWFSKGTLERFVRFVSTPEVLEMANTFDAEMSQLEGARKIYAAQGTPVGATSGAAAEASAAAADITKKELLRAIDVRLSALKQDLITSCARASSAGFNHDSVSELLHFTDHFGASRLSEACNKYMSLCQRRPDINPQHASPAPSSHWKSFEDGDLRDSCSSDMSIDEPQADHGGSSNRSTGWGSVPHTDRLSNSQHSIDVPSGSSAEQQSKPTTQQTVDKQENETETPPAPAKELSRRLSVQDRISMFENKQKEQTSTSGNSNSSGTAKVVPVKGEHRRVPSVASMDKLVRRWSSVSDMSIDLGNNDISGCNDKSENGTPAGTPTSSNLEASSKARADKDASGVKNPDTSQSWSRQKDGDKPKDSTTTNASSSSTFNTTSPPSLSAVVTEAPEKQTRSCSGDDMAIASSTESELSFDKEQGVNQGQGGTRLSEHVASNVSTENRQKTSSRPAEEAFPKHYDALTSPSSEDHVQIDKELTPVAHEVPVASEQIGRKDNRGSRLRSKEMHAAADAVVKKDRSFRTVGKTSSVVDLKSKATSNSRTNVRGSSGRDEAGSTESEVHDASSRRKSLPRKVEDVRRKVAVGSEILPQSDYSSRQGSNLSRQSSNAEQELSSLGGKVKSVNDANAIPLEQTRVTKPAKGNQDRHDELQMKANELEKLFAAHKLTTSRRGKSTDAQVDDTPRLSEVKPTQVLPEKICMKQTVTESNNFDCNELLKMVDNEGHNNSTPEKHGMLSLEDSRGKFYDQYMQKRDAKLKEDWKLQKEEKEAILKAMHESLERSKAEMQAKFSRSALQSVSRNKDQGTDSFLVEDEINSDYLSGDCSSRSADSRKHFSNKVAYTQKKSIAPIHSHKHSSRTVRSSYANRRNPPENPLAQSVPNFSDFRKENTKPSAGHSRATARAQPKSFSRSKSIIEESKSILNQDQSRGSQSMRKNLNATELRDTSSVNYNWAPSGISSNTHKSGVPKSFVRKGNGAHPVVGITGFRQPMFASVLQDDDDDFPDQQEDSPDEAKDEEYESIEENLRESDFPADSDSETPKLSQDFGNSDDPGSENGDVSFPTEASNTKFNALAANMHDLPGELPAPWSSRNPHLLPYANDTSDGDAFVDSPTGSPSPWNSHSLDQITDADVSRMRKKWGSAQLPFGGANASQQPRKDVPKGFKKLLKFGRKNRGGDGLVNDWVSASTASECDDDMEDGRDLAIGSSDDFRKSRMGYLSSYDGFVESEVFTEQEQSLRSSIPNPPANFRLREDQLTGSSIKAPRSFFSLSTFRSKGGDARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2DC29</accession>
    <name>F2DC29_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123448303</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK361444">
        <property type="protein sequence ID" value="BAJ92650.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981083.1">
        <property type="nucleotide sequence ID" value="XM_045125148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DC29"/>
    <dbReference type="SMR" id="F2DC29"/>
    <dbReference type="PaxDb" id="4513-MLOC_51197.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0359130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0359130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0359130"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0359130.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0359130.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0359130"/>
    </dbReference>
    <dbReference type="GeneID" id="123448303"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0299590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0299590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0299590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0359130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0359130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0359130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0359130.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0359130.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0359130"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0626">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DC29">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090697" evidence="7">
        <location>
            <begin position="20"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ92650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0359130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="646" mass="73379" checksum="FB1C543571F41780" modified="2011-05-31" version="1">MPWPAFLAVAAKLIVLAAAAATAANAASYARFRRRHLRRIRSPIDESADPVADFRSLPSSATAAEDDDFFFGLATAPAHVEDRLDDAWLQFATEQSGDDKESMRNQKPVDAVMASAGGDGGSQPSSRLRGDEKGTDEEKRKPLRVAMEAMLRGFEKFSEGEDTSGEDNCSHNVAAWHNVPCPQERLKFWSDPDTELKLAKETGVTVFRMGVDWTRIMPKEPTEDFKSTVNFAALERYRWIIQRVREHGMKVMLTLFHHSLPPWAGEYGGWKMEKTVNYFMDFVRLVVDRVSDLVDYWVIFNEPHVFVMLTYCAGAWPGGDPNAIEVATSALPTGVYNQALHWMAVAHAEAYDYIHSESKNAMMPIVGVSHHVSFTRPYGLFDVAAVTIANSMTLFPYIDSICDKLDFIGLNYYGQEVISGPGLKLVENDEYSESGRGVYPDGLLRILLKFNERYKSLNIPFIITENGVSDETDLIRKPYMLEHLLAIYAAILMGVRVLGYLFWTTSDNWEWADGYGPKFGLVGVDRANNLAREPRPSYYLFSKVVTTGKITRHDRTSAWRELQKAAIQKKTRPFYREVDKHGRMYAGGLDRPIERTFVLRDWRFGHYEMEGLQDPLSRFIRCIMRPFPRKKIHYIEDDAISYSISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2DE54</accession>
    <name>F2DE54_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123444028</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362171">
        <property type="protein sequence ID" value="BAJ93375.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976562.1">
        <property type="nucleotide sequence ID" value="XM_045120627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DE54"/>
    <dbReference type="SMR" id="F2DE54"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069460"/>
    </dbReference>
    <dbReference type="GeneID" id="123444028"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0055790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0055790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0055790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444028"/>
    <dbReference type="OrthoDB" id="48036at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.670">
        <property type="entry name" value="Formiminotransferase, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.990.10">
        <property type="entry name" value="Formiminotransferase, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037070">
        <property type="entry name" value="Formiminotransferase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012886">
        <property type="entry name" value="Formiminotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037064">
        <property type="entry name" value="Formiminotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022384">
        <property type="entry name" value="FormiminoTrfase_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051623">
        <property type="entry name" value="FTCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12234">
        <property type="entry name" value="FORMIMINOTRANSFERASE-CYCLODEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12234:SF5">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07837">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01222">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55116">
        <property type="entry name" value="Formiminotransferase domain of formiminotransferase-cyclodeaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formiminotransferase N-terminal subdomain" evidence="1">
        <location>
            <begin position="17"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ93375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="316" mass="34619" checksum="E488A564018B133E" modified="2011-05-31" version="1">MEGKHTDQANVKAKHSKVICCKLYISESRNAAIVDAISRIGQKNPEVVLLSKFDDKYYNRVRYTLVSYITSESSAGEAVFGPIRKVLLEMIEAAFSAINLQAHSGTHPRIGVVDDISFHPLSPAATMEDAAQLAKLVASDIGNGLQVPVFLYAAAHPTSKSVSAARRELGYYRPNHKGVQWAGQVLPHTLPVKPDVGPAHVSRERGATMVGATPFVDNYNVPIFCKDVPTVRRITRRVTGRSGGLPTVQALALFHGDNCTEIACLLDPDHVGADQVQWLVEQIAEEQGLEVDKGYFTDLSKDMMLERYFEMISAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFG2</accession>
    <name>F2DFG2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123447451</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_045124055.1"/>
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    <dbReference type="AlphaFoldDB" id="F2DFG2"/>
    <dbReference type="SMR" id="F2DFG2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337120"/>
    </dbReference>
    <dbReference type="GeneID" id="123447451"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0281150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0281150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0281150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0337120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0337120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0337120"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:nucleosome"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of DNA recombination"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Putative serine/threonine protein kinase IREH1"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
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        <property type="entry name" value="Histone_H1/H5_H15"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF29">
        <property type="entry name" value="OS03G0711600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATHOOK"/>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="H15"/>
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        <location>
            <begin position="19"/>
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        </location>
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        </location>
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        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337120.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="23922" checksum="83D1B166C189D2ED" modified="2011-05-31" version="1">MATSSGGQSSPRAASDLAVIPSYPEMIVEAIASLREPNGSSQAAIARRIEAAHGAGGDLPPSHPALVAAHLSRMSAAAELVAFAGGKYALPPPPSRSPAEEEDMEPEREEEEEEDDDDDSEDAPLPLPPPPPAKRGRGRPPKVRPPGYPGGPPATIGAPPSDGLAVAVATAPRRRGRPPKPRDPDAPPKIPRPRGRPRKNPLPDGMVPVPRPVSTTTKPRPQFAEVGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFQ9</accession>
    <name>F2DFQ9_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123406370</name>
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    <organism evidence="2">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362726">
        <property type="protein sequence ID" value="BAJ93930.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK368282">
        <property type="protein sequence ID" value="BAJ99485.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955817.1">
        <property type="nucleotide sequence ID" value="XM_045099882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DFQ9"/>
    <dbReference type="SMR" id="F2DFQ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628610"/>
    </dbReference>
    <dbReference type="GeneID" id="123406370"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0628610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0628610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0628610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406370"/>
    <dbReference type="OrthoDB" id="1918565at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF142">
        <property type="entry name" value="OS02G0812600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ93930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0628610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="287" mass="31675" checksum="540540792E279528" modified="2011-05-31" version="1">MEEACEIARLPEELVSAALARTSPRDACRAAAVSQAFRAAADSDDVWAGFLPPGGLPPLADGEPPGPAPPSSKKELFLRLSAGPALLQDRLVSVWLDRETGAKCYMLSARNLFIVWGDTPEYWSWIPLEDSRFSEGAELLDVCWLEIHGKIHSKMLSQDTTYAAYLVFKMADNFYGLNFPVQEASVSSGATNLTRKVCIQAGHRDNNNYDSDDDDDDDDDEYDVYLKENVTFPQKRVDGWLELELGEFLSEGGDDGEVSISLTETKGGMWKSGLIVQGIEIRRKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DHM9</accession>
    <name>F2DHM9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123446533</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Seed</tissue>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK363396">
        <property type="protein sequence ID" value="BAJ94600.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372567">
        <property type="protein sequence ID" value="BAK03765.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979043.1">
        <property type="nucleotide sequence ID" value="XM_045123108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DHM9"/>
    <dbReference type="SMR" id="F2DHM9"/>
    <dbReference type="STRING" id="112509.F2DHM9"/>
    <dbReference type="PaxDb" id="4513-MLOC_78462.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380720"/>
    </dbReference>
    <dbReference type="GeneID" id="123446533"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0380720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446533"/>
    <dbReference type="eggNOG" id="ENOG502QWGU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054280_0_0_1"/>
    <dbReference type="OMA" id="WVRQQQG"/>
    <dbReference type="OrthoDB" id="780613at2759"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DHM9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37234">
        <property type="entry name" value="OS03G0319200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37234:SF1">
        <property type="entry name" value="OS03G0319200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ94600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK03765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="419" mass="45325" checksum="B83A19E63EF901BE" modified="2011-05-31" version="1">MQPTESAQRRASSTAATAPRRSMGCMAGLLRLLSPYHRHRKRLTAKNATLEASLPPPSPPPMKKPGASPSPPPVKKPGASLSPTQAPEKPQTPTAVRRRRSCEAPRSPTIAPEYRRASCDSPRPPPPAIVARLMGLEESAPASPATTPRPRPLPTRPPPPPPPEMAAEKRRKLLGALEKCDDDLQTLRRIIAAVRAAELRSAAASDVSPAPAGEGKGGKWMDGSPRAVAQHPSPDSVLDAITSPRFPCRKRSSPCTHLDADSKPEITRSSNDALIVRSKIVKPSRTLVFSGDYCKIKQYCNELHAMAMHHHPATVAAVEGMPRWTPSAAEAKGWQHRRRWGLQGEERSRAMVESVGEVWGESAGEERWEAGLVGAALERAIMQELVLDVVTELLAHSEGPFGHGHHHGGGAMCRKRLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DJ69</accession>
    <name>F2DJ69_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123426560</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <dbReference type="EMBL" id="AK363937">
        <property type="protein sequence ID" value="BAJ95140.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966354.1">
        <property type="nucleotide sequence ID" value="XM_045110419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DJ69"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0163380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0163380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0163380"/>
    </dbReference>
    <dbReference type="GeneID" id="123426560"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0134730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0134730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0134730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0163380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0163380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0163380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426560"/>
    <dbReference type="HOGENOM" id="CLU_031332_3_0_1"/>
    <dbReference type="OrthoDB" id="2019938at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04897">
        <property type="entry name" value="ACT_ACR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04926">
        <property type="entry name" value="ACT_ACR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040217">
        <property type="entry name" value="ACR1-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096:SF55">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ACT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="123"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="329"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ95140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0163380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="443" mass="48775" checksum="F5A9FE6AF1BA73E4" modified="2011-05-31" version="1">MALTDDHDEYAKLVRGMNPPRVVIDNDASDDATVIRVDSVNSHGTLLAVVQVIADLNLVIRKAYFSSDGSWFMDVFNVTDRDGNKVLDTPTISYIQKTLEAEDCYYPEVRNTVGIVPSEDYTSIELTGTDRPGLLSEVCAVLAGMQCAVRSAELWTHNTRVAAVVQVTDAAKAAGGAIEDDARIADISRRLDNLLRGQNGVRAAAAASLTHKERRLHQMMFEDRDYGAAGPPDPRTEVSVTHCAERGYTVVVVRCRDRPKLLFDTVCTITDMQYVVHHGTVSSEPAGGAYQEYYIRHVDGHPVSTEAERRRVVQCLEAAVERRTADGLELEVRTDDRAGLLSDVTRIFRENGLTIRRAEISSEDGEAVDTFYLSDPQGHPVEAKTIEAIRAQIGEATLRVKNNPLADDGGSTSEVAAGSTAFLFGNLFKFYRPFQNFGLIKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZ23</accession>
    <name>F2DZ23_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429197</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369143">
        <property type="protein sequence ID" value="BAK00345.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969189.1">
        <property type="nucleotide sequence ID" value="XM_045113254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DZ23"/>
    <dbReference type="SMR" id="F2DZ23"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102130"/>
    </dbReference>
    <dbReference type="GeneID" id="123429197"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0084160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0084160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0084160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0102130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429197"/>
    <dbReference type="HOGENOM" id="CLU_009313_4_0_1"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001496">
        <property type="entry name" value="Os11g0284032 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK00345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="559" mass="61787" checksum="D2AE48597F0526E1" modified="2011-05-31" version="1">MALGGFVDWRGNAINKQVHGGVRAAWFLYVLTVASNVVIIPNQLNIVTYLHGTMHMGVSGSATTATNFFGATSGFAMIGAFLSDSYITRSRTMLLFGPFMFLGYGLLALQAYLPSLHPPPCNIETEVNNCKEVHGWNATLLYTALYMSAFGDGFIRVCLASLGADQFDHEDPTESRQQSSFFNWYTFGISFGGFIGLIFIVWLENYKGWDIGFGVCAILILLGLLVVAAGLPFYRNQVPKGSPLTPILQVLVVAFRNRKLEHPENLEEALESSAGTMTSSIDKLSQTKTLKFLDKACINRGENGAWSVCSVTKVEETKIVLRLLPLFISSMISYVPTIIIFTFTVQQGGMTNTRLGKIHVSPATLFIIPTIFQLIMLAVYDQFLVPFLRRCTGYVGGVTHLQRVAIGFASMLLASVIAAIVEKKRKQVVVPMSLFWLAPQFFLLGVADVTSFTGLLEFFNAESPRGMKSIAIALLWCEVGLASLMSTLLVKTVNIATRHEQHGGWLEGTSLNNSHLDLFYWVVAILGLLGFFNYLYWAKKYVYQHNPRIVEATANQGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZ25</accession>
    <name>F2DZ25_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123426084</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369145">
        <property type="protein sequence ID" value="BAK00347.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965794.1">
        <property type="nucleotide sequence ID" value="XM_045109859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DZ25"/>
    <dbReference type="SMR" id="F2DZ25"/>
    <dbReference type="FunCoup" id="F2DZ25">
        <property type="interactions" value="1524"/>
    </dbReference>
    <dbReference type="STRING" id="112509.F2DZ25"/>
    <dbReference type="PaxDb" id="4513-MLOC_4757.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0146690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0146690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0146690"/>
    </dbReference>
    <dbReference type="GeneID" id="123426084"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0146690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0146690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0146690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426084"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_15_1_1"/>
    <dbReference type="OrthoDB" id="745501at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DZ25">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000679">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g03800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002102">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF512">
        <property type="entry name" value="REPEAT (PPR) SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="554"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="771"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK00347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0146690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="861" mass="94719" checksum="8870D81CF632271F" modified="2011-05-31" version="1">MATSISSSPGPLAPLLPPRSLLSFPPPPPPATTTSTAIHGTAPRLRLLPNAADPRAAHAVATKSGTAAQLDARLANAVMCGYIRAGRLTDALEVFDRMTARDAASYSALISGHARLGSPVSAAEALFRSMRLAGLAPTKYTFVGLLTACIRRGNPRLGTQVHALAAKGRYIGGSLLVANALLGMYVKCGRLEDALRMFHGMEERDVSSWNTVLSGLVELGRYEEAFELFGDMRTSDVAVDRFSLSALLAAATEGFCLPHGAAVHALSLKSGLEVDLSVGNALIGFYAEHGDSVEDMVGVFQRMPVKDVISWTGLLNGYMEFGLVDNALGVFYRMPERNFVTYNAVLTGFCRNKESARVTFARKAGLQGLGLFRQMLENGLEMSDVTVTGVLNACAIAADRKISEQVHTFVIKCGCGSSPWIDAALIDMCIKCGRSGDARLLFEHWRHQESFHIAWSSLLLSSFRDGEYEKAFSTFLQMFRNSDIQFIDEFLLTNVLGVCGALGFMELGKQMHLLAAKSGLLRACGVGNAIVSMYGKCGQLENAVTFFQRMPHRDLVSWNALITAHLLHRQGDEIWDTWSEMERLVIKPDSVTFLLIISACSCTNSDSADACMELFHSMSSKYNTEPAMEHFAAVVNVLGRWGHFDEAEQFIASMPFKPSAIVWRSLLETCSKQSNMTLRRRAMNHLLALEPQDPSTYVLASNLYSESAKWHCSENTRLEMREKGIHKIPARSWTFDDNAIHSFFARDRSHPQSKDIYAGLDVLTLECMKAGYEPDTTFVLHDVEEYQKRYFLMYHSAKLAATYGLLMAGSGKIIRVVKNIRMCGDCHSFLEHASAATGKEISVRDSNGFHIFRAGICSCRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZF2</accession>
    <name>F2DZF2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123450668</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAK00474.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_045127809.1"/>
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        </location>
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        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
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        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
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        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAK00474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089170.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="147" mass="16056" checksum="8D26CC1D40166CF1" modified="2011-05-31" version="1">MSEKPSDDTTGQVRPEGDVSDVKVETANQNKGNEMPSAQQEEAVIKKKYGGVLPKKSPLISKDHERAYFDSADWALGKQGGHPQKPKGPLEALRPKLQPTQQQARSRRFLHASTDSEEGANSPTEATTPNQESTEEAKAAENKESTE</sequence>
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        <name evidence="3" type="primary">LOC123439397</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
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                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK371385">
        <property type="protein sequence ID" value="BAK02583.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972049.1">
        <property type="nucleotide sequence ID" value="XM_045116114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E5G1"/>
    <dbReference type="STRING" id="112509.F2E5G1"/>
    <dbReference type="PaxDb" id="4513-MLOC_6259.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0232050.1.CDS1"/>
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    <dbReference type="GeneID" id="123439397"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0192600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0192600"/>
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    <dbReference type="HOGENOM" id="CLU_167986_1_0_1"/>
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    <dbReference type="OrthoDB" id="652307at2759"/>
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        <property type="component" value="Chromosome 3H"/>
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        <property type="entry name" value="DUF3339"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK02583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0232050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="70" mass="7669" checksum="C9D36409E0D9EDA1" modified="2011-05-31" version="1">MQDWAGVFIPLVLFILLSPGLLFQIPGKCRIIEFGNFHTSAVSIIVHSIIFFSFAAIFLVAIGVHIDLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>M0UDW8</accession>
    <name>M0UDW8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123425284</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the N-terminal section; belongs to the HPPK family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045108960.1"/>
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        <property type="nucleotide sequence ID" value="XM_045108961.1"/>
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        <property type="nucleotide sequence ID" value="XM_045108963.1"/>
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    <dbReference type="SMR" id="M0UDW8"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0124660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0124660"/>
    </dbReference>
    <dbReference type="GeneID" id="123425284"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0124660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0124660"/>
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    <dbReference type="KEGG" id="hvg:123425284"/>
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        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UDW8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.20:FF:000006">
        <property type="entry name" value="Dihydropteroate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.560:FF:000003">
        <property type="entry name" value="Folate synthesis bifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0124660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="542" mass="58793" checksum="6BBE556D520BF65E" modified="2013-04-03" version="1">MLHAKETLRKMYSVAKTYYCGGLAAPNPFPAALDLRHSLSSSRTAPGYANPCRARSYTRSALAGGPAAGQEIVIALGSNVGDRISSFDRALRLMKDSGIKITRHACLYETAPKYVTDQPRFLNSAVRGTTKLGPHDLLKKLKEIERDIGRTAGIRYGPRPIDLDILLYGDSQINTETLTVPHERIHERQFVLAPLVDLLGSSADDHIEKSWHSLSKCSGGFFDVWNELGGESLIGTQGIKRVMAVGNRLLDWSERTLIMGVLNLTPDSFSDGGKFQEVEAAIAQVKLLISEGADIIDIGAQSTRPSATRLSANEELERLVPVLDAVTKIPEMQGKLLSVDTFYGQVAAEAVKRGATMVNDVSGGQLDPSILEVVAELGVPYVTMHMRGDPSTMQSPQNLQYDDVCKEVASELYARLREAELSGIPLWRIVLDPGIGFSKEPKHNLEILNGLRSIRKEMGKVSLAASHVPILLGLSRKSFLGEICNRADPVERDAATVAGNAIGIMNGANILRVHNVGYSVDGAQIADALRMAKPKSIPPAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>M0UQC1</accession>
    <name>M0UQC1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123403190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953078.1">
        <property type="nucleotide sequence ID" value="XM_045097143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UQC1"/>
    <dbReference type="SMR" id="M0UQC1"/>
    <dbReference type="STRING" id="112509.M0UQC1"/>
    <dbReference type="PaxDb" id="4513-MLOC_13074.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612270"/>
    </dbReference>
    <dbReference type="GeneID" id="123403190"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0507660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0507660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0507660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0612270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403190"/>
    <dbReference type="eggNOG" id="ENOG502QVP8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0UQC1"/>
    <dbReference type="OMA" id="HEEMEFC"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UQC1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF179">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="442" mass="47922" checksum="61B82D6042CAE627" modified="2013-04-03" version="1">METTGGKMLKPAYSAPHPLAGEMVPLRLFDRAAMDIFVSLILVYPAPTPSNEALVEGLRRALAPYPHLAGRLAVDHSGRRSIHLSNDGVLVLDAEVPVDMASVVADGCFITATEGLYPALPLPEENVGAALLQIKLSRYKCGGLVIGIIHHHHAADGHSFSNFLTTWASAVRQASEFAAPSPFLDRAATAVPRSVPTPAFDHRCTEFSGEEDDRRSSYSHPRCKIKNLTLRFTAQFVRELKARVGAPCSTFQCLLAHVWKKITAARRLKPDEFTQVRVAVDCRARASPAVPPDFFGNMVLWAFPRLQVRDVLGWTYGGVVGAIRDAIGRVDAAYIQSFVDFGSVADASWEELAATAPTAGTMLCPDLEVDSSLGFRFHQIDLGNGPPSAFLMPDLPVEGLMTFVPSCSAKGGLDVHMAVAEDHVAELEHICYSLDERATPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>M0W5H3</accession>
    <name>M0W5H3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973028.1">
        <property type="nucleotide sequence ID" value="XM_045117093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0W5H3"/>
    <dbReference type="SMR" id="M0W5H3"/>
    <dbReference type="FunCoup" id="M0W5H3">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0W5H3"/>
    <dbReference type="PaxDb" id="4513-MLOC_43984.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0060400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0060400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0060400"/>
    </dbReference>
    <dbReference type="GeneID" id="123440525"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0047930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0047930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0047930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0060400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0060400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0060400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440525"/>
    <dbReference type="eggNOG" id="ENOG502QVMR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0W5H3"/>
    <dbReference type="OMA" id="GPIMWRC"/>
    <dbReference type="OrthoDB" id="21648at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0W5H3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd11650">
        <property type="entry name" value="AT4G37440_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038745">
        <property type="entry name" value="AT4G37440-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34057">
        <property type="entry name" value="ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34057:SF13">
        <property type="entry name" value="OS05G0365200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0060400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="544" mass="61045" checksum="600E9F8E3AC08577" modified="2013-04-03" version="1">MPAAPAVGSNGHDAHGRAGCRESLLRPRPHPPARAGVPVPVRGDAVQAIESSGDTVSDNLRFHEDGHGDSTECSSSFGPSCSGSDDETRSGTHDMEVDSPFLGNFNVDGAASLPKKPRPKQVTAEWRNAVRPIMWRCQWLELRMKELSSQVAKYDRELALISHEKDLRSEMIAADSSDPELAKLDVQGHDRNIMKRRRRQRLEDIVDTSLYMDGHQIISYYHESKNRNYGAETDGLLIYDDAVFEDTKRGVPDNTLLGSKETDRVLEQYSLTDILRTIDSVQSRVLKLQVHLSKVCSNRTQVKVPPKSQKAQTQLASCKKDGHRPKKKRDLNSLLQEEDTHRPLVGVPTALSDRCTDYVMEHGKRNIAEEVATQPYAMKVTFETLFGADNPLIHHTHLGELYKENADDVLIDNRAAQVEGYQQFEKVKQEAQNHVDLANKVANTLLSRGEETVARQVVKLEPVYEIAPSVKQVSPANKRIKKPKKKSGSSLPPLKEQIEKSPDVPAKKRTVKDLHDFKNEKPVFVAVDTRRSQRVRKPKKYGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>M0WZI8</accession>
    <name>M0WZI8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123403853</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953709.1">
        <property type="nucleotide sequence ID" value="XM_045097774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WZI8"/>
    <dbReference type="SMR" id="M0WZI8"/>
    <dbReference type="STRING" id="112509.M0WZI8"/>
    <dbReference type="PaxDb" id="4513-MLOC_55848.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609880"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609880.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609880.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609880"/>
    </dbReference>
    <dbReference type="GeneID" id="123403853"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0505700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0505700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0505700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0609880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0609880.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0609880.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0609880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403853"/>
    <dbReference type="eggNOG" id="KOG0749">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="AYQRQYK"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF47">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="12 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="78"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="183"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="283"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0609880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="380" mass="41415" checksum="F010484E1B1FD330" modified="2013-04-03" version="1">MADRANHPSVMQKFGGQFHLSSSFSEGVRARNICPSVPSYERRFTTSSYMTQSLGIRAPMMSSSPLFANAPPEKKGAKNFAIDFLMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKAGRLSEPYKGIGDCFGRTIKEEGFGSLWRGNTANVIRYFPTQALNFAFKDYFKRMFNFKKDKDGYWKWFGGNLASGGAAGASSLFFVYSLDYARTRLANDAKASKGGGERQFNGLVDVYRKTLKSDGIAGLYRGFNISCVGIIVYRGLYFGLYDSLKPVLLTGTLQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSLDAFNQILAKEGAKSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>M0X7Z0</accession>
    <name>M0X7Z0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoyl(octanoyl) transferase</fullName>
            <ecNumber evidence="3">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123448769</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044981704.1">
        <property type="nucleotide sequence ID" value="XM_045125769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0X7Z0"/>
    <dbReference type="SMR" id="M0X7Z0"/>
    <dbReference type="FunCoup" id="M0X7Z0">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0X7Z0"/>
    <dbReference type="PaxDb" id="4513-MLOC_57763.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380320"/>
    </dbReference>
    <dbReference type="GeneID" id="123448769"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0316360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0316360.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0316360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0380320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0380320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0380320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448769"/>
    <dbReference type="eggNOG" id="KOG0325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0X7Z0"/>
    <dbReference type="OMA" id="GEVTYHC"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0X7Z0">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000063">
        <property type="entry name" value="Octanoyltransferase LIP2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010925">
        <property type="entry name" value="PRK14345.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF15">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIP2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0380320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="242" mass="26198" checksum="262DC21FBBC30B9C" modified="2013-04-03" version="1">MSGGAGRRVLEAWKLGVVKYGDALRLQERLVADRRAGRVPDLVLSLQHPPTYTLGKRRTDHNLLVPESSLAGLGAELYRTERGGDVTFHGPRQAVLYPILSLRDIGLGARRYVEGLESAMIEVASLYGVKARPGGACETGVWVGDRKIGAIGVRISSGFTCHGLAFNIDPDLGYFKHIVPCGIADKEVTSLRREAAVELPPDEVIHDQLVQSLATTFRLGDVKVKDDSECADMISSAATVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>M0XLR3</accession>
    <name>M0XLR3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123444863</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044977666.1">
        <property type="nucleotide sequence ID" value="XM_045121731.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977674.1">
        <property type="nucleotide sequence ID" value="XM_045121739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XLR3"/>
    <dbReference type="SMR" id="M0XLR3"/>
    <dbReference type="PaxDb" id="4513-MLOC_6117.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0071760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0071760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0071760"/>
    </dbReference>
    <dbReference type="GeneID" id="123444863"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0057910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0057910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0057910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0071760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0071760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0071760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444863"/>
    <dbReference type="eggNOG" id="KOG4046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XLR3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.210:FF:000002">
        <property type="entry name" value="Ribonuclease P protein subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0071760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="319" mass="35855" checksum="29BC0836D6B9F5F9" modified="2013-04-03" version="1">MSTISDQKKRTLEAIQQRYAAAKAKKLQDEQPKCPKNKESTPKAKFDARRKGKTPESTPSRTSGQLPTFRAQGNSGHQQKPSASSGGEINPIYSEISFALHENLSQDDYSDLDSTDVVHSVVYDIIQKGGEAGKIAKGSKKLKLDRGILLDNYVQRGPILVDAQSRSLLIHSKRSKRHMSLKQHKKCGSFDLHDTFRRFDLYKPMHEMWKEYMRELTKSTPKKQLSENLLSADLHGALIIVAQCKAASYQGVSGIMIRDTAETFGIISEDNRFRVVPKAGSVFVLQADCWKVTLIGDKLSPKEKLKESQRMQHAQSLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>M0YR26</accession>
    <name>M0YR26_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123440660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973156.1">
        <property type="nucleotide sequence ID" value="XM_045117221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YR26"/>
    <dbReference type="STRING" id="112509.M0YR26"/>
    <dbReference type="PaxDb" id="4513-MLOC_7195.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308660"/>
    </dbReference>
    <dbReference type="GeneID" id="123440660"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0257730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0257730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0257730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0308660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0308660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0308660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440660"/>
    <dbReference type="eggNOG" id="ENOG502S74R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YR26"/>
    <dbReference type="OMA" id="ADPREHN"/>
    <dbReference type="OrthoDB" id="619048at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YR26">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF41">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="85"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0308660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="401" mass="45106" checksum="1744306C2E19A70B" modified="2013-04-03" version="1">MPPSETKRRDWADLGDGVAGMIADRVLAYDVADYLRFRAVCSPWRRCCADPRAHGDLDRRFHPRRWLMLREPRERIDSPNVRRFLNSSTGECIRVHLPGLHNQEVLALSSEGLLVVLDWPRSTKVHLLNPLTRHLTQLPPLMALLSYQGYECFLYTSAFDTHFPAWGSGILNDDSTVVLCFSRLNMLGMAKPGDDHWTLLNFHSPSPVALGTASSMIAGRFYCVTTDGVMVLEDQPPRLELVAKLPMPVSPVADTVHLMDSAGELMLVHRRFSRHHDTNNVAKRRYDVYRVDLGAGTLCRVNSFGGGAHALFIGLYCSLSVPIKLFPAGSIRGDTIYLSFDIAERSKTEGYHLADRSTISSDNLNCHLVLQPHTLVDCLSLCTTGRIWCHFEKHLVSQCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>M0YYK1</accession>
    <name>M0YYK1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123427234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967166.1">
        <property type="nucleotide sequence ID" value="XM_045111231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YYK1"/>
    <dbReference type="STRING" id="112509.M0YYK1"/>
    <dbReference type="PaxDb" id="4513-MLOC_74471.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0181540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0181540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0181540"/>
    </dbReference>
    <dbReference type="GeneID" id="123427234"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0181540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0181540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0181540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427234"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YYK1"/>
    <dbReference type="OrthoDB" id="664960at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YYK1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF204">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="177"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0181540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="919" mass="104210" checksum="D79AAAA1D949CAC2" modified="2013-04-03" version="1">MADVISAAGSCLQPLCECLDGTGMLDVAAREVASFLHLKSNWSDLDKAEKLLLAVETTVRARVTAELDKLNICDPQVQVWLRRVEELQLDAIDEDYSQLMKYSCLGQCTIHAHRRTSVGRRVLEALDEANKLIEEGRRFKKFGFKPLPRIVDPLPQIETFGLETMLSQLHDLFEKGDSNIIGVWGQGGVGKTTLLHVFNNDLEKKAHDYQVVIFIEVSNSEALNTMEIQQTISERLNLPWNDAEPIAKRARFLIKALARKRFVILLDDVRKKFRLEDVGIPTPDTNSRSKLILTSRYQEVCFQMNAQRSLIKMQILGNDASWELFLSKLSKEASAAVESLGLQNTSREHAMAIARSCGGLPLALNVIGTAVAGLEESEWKSAADAIATNMENINGVDEMFGQLKYSYDSLTPTQQQCFLYCTLFPEYGSISKEQLVDYWLAEGLLLNDCEKGYQIIRSLVSACLLQASGSMSTKVKVHHVIRQLGLWLVNKSDAKFLVQSGMALDNAPSAGEWNEATRISIMSNNITELSFSPKCKKVTTLLMQNNPNLNKMSYGFFRTMSSLKVLDLSYTAITSLPECDTLVALEHLNLSHTHIMRLPERLWLLKELRHLDLSVTVALEDTLNNCSKLHKLKVLNLFRSHYGIRDVDDLNLDSLKELLFLGITIYAEDVLKKLNTPRPLAKSTHRLNLKYCADMQSIKISDLSHMEHLEELYVESCYDLNTVIADAELTTSQLQFLTLSVLPSLESVLVAPMSHNFQYIRKLIISHCPKLLNITWVRRLQLLERLVISHCDGVLEIVEDEEHYGEQMKMQDHASDEQEDHAMVKTSRNDTGQSDFPKLRLIVLTGLKKLRSICKPREFPCLETLRVEDCPNLRSIPLSSTHNYGKLKQICGSVEWWEKLQWENRDEVAYLDSKYFIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GQ58</accession>
    <name>A0A287GQ58_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123451531</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044984193.1">
        <property type="nucleotide sequence ID" value="XM_045128258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287GQ58"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091510"/>
    </dbReference>
    <dbReference type="GeneID" id="123451531"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0075390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0075390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0075390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0091510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0091510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0091510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451531"/>
    <dbReference type="OrthoDB" id="439326at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287GQ58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35738">
        <property type="entry name" value="OS05G0577800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35738:SF3">
        <property type="entry name" value="OS05G0577800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0091510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="47180" checksum="19E9DAABD008B114" modified="2017-11-22" version="1">MGVLWSKPEPPPPVVLVPPLFDYPPIAARTRMLVPAYELMFGKLPLRTLFEDYFDNAGTVSSRIMLKPLEDPHVDLVANVSAAGDKASGTEVKGDALFRWQKDLYDPHTFVDIHVSTSEPMLKLRSCAYFPEYRLGAFGTIPLLMGNNRLSSEDYAVLGVRYGSENLSIGASFVPLPESAEVPYGAWLVGRKGSLCAGALYKPLIGGNKHPMPYTDPKNWNYAVSYGVASTSPLSPSFNFSLELARNTQLIASFYQHLVVQRRIKNPFEDNQVVGITNYIDLGLELATGIDKSKPAESGNNSLFQLGASWQANKNFLLKGKLGPSKSSVALAFKSWWRPSFTFSVTAENDHSKGTTSYGFGIRVEDLRKPSYERADPNYVMMSTNKEHLAPAALREFGQRPMFQADVESGNFDHLPTELRPISKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287Q0R0</accession>
    <name>A0A287Q0R0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor</fullName>
            <shortName evidence="6">bHLH transcription factor</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Basic helix-loop-helix protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449961</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983280.1">
        <property type="nucleotide sequence ID" value="XM_045127345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287Q0R0"/>
    <dbReference type="SMR" id="A0A287Q0R0"/>
    <dbReference type="STRING" id="112509.A0A287Q0R0"/>
    <dbReference type="PaxDb" id="4513-MLOC_13653.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415230"/>
    </dbReference>
    <dbReference type="GeneID" id="123449961"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0346040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0346040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0346040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0415230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0415230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0415230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449961"/>
    <dbReference type="InParanoid" id="A0A287Q0R0"/>
    <dbReference type="OMA" id="CTNQRNT"/>
    <dbReference type="OrthoDB" id="623055at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287Q0R0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045084">
        <property type="entry name" value="AIB/MYC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11514">
        <property type="entry name" value="MYC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11514:SF104">
        <property type="entry name" value="TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="59"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="27768" checksum="294C18DEBF89B958" modified="2017-11-22" version="1">MEPDMDVSFDFSWEALVLELGDANNMYLPAEDSLSGLYDDSTSSPDGATSRPTTKATTSLERKNIINERRRRRTLNEKLYAIRRVVPNITKMDKASIIQDAIAYIEELQEQERQILAALRTDGSTAVVKAEDAASTGSNGVDHGAGSSPGKKMRRTTSASSINGALCSGATQPVQILELEVTQVAEELIMVNMRHGNAHEAIAKVCEALESLCLKVISTSITAVASGIVHNLVVETEGLHGAQTIKEMIQTHSAILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287T3X0</accession>
    <name>A0A287T3X0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box protein AT5G49610-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287T3X0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287T3X0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33186:SF16">
        <property type="entry name" value="F-BOX ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33186">
        <property type="entry name" value="OS10G0136150 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="1">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="22186" checksum="9FFB36F9FDBBA899" modified="2017-11-22" version="1">MRRKDGCIRLPLRVASIKPSILIGSAVYWLIYGGHILVFDIERQTLGVIDRPADVHWTDIWSFQLLRTDNGCGLGLAVMSGLSIHLWERKLNTGGVVGWVLLQKTIPQEIMFPQRMRSDHKQAAVVGYDYDEESNVVVLATFTGDFMLRLESMRFRRISERNVWDNNTIHYPYRNFYTVGSAVGWKWLDLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287TN77</accession>
    <name>A0A287TN77_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BEACH domain-containing protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123401261</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950944.1">
        <property type="nucleotide sequence ID" value="XM_045095009.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950945.1">
        <property type="nucleotide sequence ID" value="XM_045095010.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950946.1">
        <property type="nucleotide sequence ID" value="XM_045095011.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A287TN77"/>
    <dbReference type="STRING" id="112509.A0A287TN77"/>
    <dbReference type="PaxDb" id="4513-MLOC_9900.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0566040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0566040"/>
    </dbReference>
    <dbReference type="GeneID" id="123401261"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0566040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0566040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0566040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401261"/>
    <dbReference type="InParanoid" id="A0A287TN77"/>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287TN77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046852">
        <property type="entry name" value="Neurobeachin_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF112">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20425">
        <property type="entry name" value="Neurobeachin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2336"/>
            <end position="2392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2450"/>
            <end position="2482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="2173"/>
            <end position="2207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="2337"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2348"/>
            <end position="2360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2361"/>
            <end position="2373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2374"/>
            <end position="2389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="2450"/>
            <end position="2462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3237" mass="361770" checksum="9593AEC062777FF1" modified="2017-11-22" version="1">MNIVKGVADLLRKSTPSSPASGGGGAGAGGGGPSGGGGGTGSPSADKFAAAPSPRVRFSDTGEEAVLNTLWQRYENAIDKAEKKKSLQIFVLHFIQAFKDWEPHHIDQSIDRESVSDDTVIGCSGGHPSEVILILVQEISHLTSFVTESSSCPESSANLSEQSTDLGLSTEVLPVLEWFTIVTRSVHNCRVFSYYGGVQKVTALLKAAVVKLKTLTSLLATDEQLSNRTVENMRMMQKILVYIVTIISNFMDLEPAATRISQVANSTDHIPSNNLATVTPNTTRRFVSDRNWQQKAIVSVMEAGGVNWLVELLRVIRRLNLKDQWTDLSLHFITLYALRSTISENTRAQNHFRSIGGLEVLLDGLGLPSSKFSVSKHSFVPSDERSDILQLQILSLEILREAVFGNVNNLQFLCENGRIHKFANSICWPAFMFQEFHQQKFLDSEVPNWKLDKRSTGNSPTLESFPSPVDILNTTEWNEYSVKLSKALCSFLLPPNEIRYCPGSTVTRISLPISLAYWEQCARWIIKILSTVFPCIKACASETELPNHIRILSNTLQHYMLSTFRKVLISAPGLLKSFREEGLWDLIFSEKIFYFGSSVECIHQIIGETETQTDRFADATESTGSKSNLPDVNILQSEAISFLEFAATLNENTNNLPECSALLSALELCTNGPGLVITILKSFHVILQLATEQTLVSFKSLDVLTRVLKVACLQTQELRKAHPSDDLRGNGSQSNNVQVDSLDQRIKNALTCVELAFNLFKEYVTISDLGRKIILHNANCIGCLFDLFQEENLRKQVLEQVLALYRLSPSSAQDHTAKLQLCSKYLETFTRAKEHEKNFAEMSIDLLVTMREIILIDRAYYQNLFRNGECFLHIVSLLNGTFDEAVGEQLVLNVLQTLTSLLAENDESKATFRMLVGVGYQTLQSLLLDFCKWLPSRKLLDALLGMLVDGTFEINEKTTIKNEDVIILFLNVLQKSSISLQHYGLVVLQQLLKGSITNRTYCFKAGLLSFLLDWFSVEEWEDTVIKIAELIQIIGGHSISGKDIRKMFALLRGEKISVKQKHSSLLLTSLSHMLKEKGPEAFFEFSGHDSGIEVKSPVQWPYSKGISFCCWLRVESFPENGMMGLFSFFTENGKGCLAMLGKNTLVYESVSQKNQCVLLPLSLPTKQWKFLSVTHTVGRAFSGGSQLRCYVDGDLVSTEKCRYAKVNEVMTRCSLGTELMPIGEEPASLGFEGTFAFTGQMGPVYAFSDALSSEQIRGIHNLGPSYMYSFLGDQNLLMNNDSLYKGILDARDGISSKMIFGLNAQASNNRTLFNVSSVLDSLDKMKVEATIMGGTKLCSRRLLQDIIYCVGGVSVLFPLLIHFDDAITHSGESPTGDQLAGQVIELVASVLDGNVANQQQMHLLSGFSILGFLFQSVSPQLLNFKTLSALKHLFNVLTNCGMSEILLKDALSQIYLNPHIWAYSSYEVQRELYLFLIQYFETDGKLLPMLCGLPRIIDIVRQFYSEKLDSRSSKPLLHLVTELVIGERPHIEEIRKIRLLLLSLAEMSLRLKVSSHDIRALVSFFEKSQDVACIEDVLYMIIRALSQNSLLSSFLEQVNCVGGCYIFINLLKREFEPIRLLGLQLLGKLLVGVPSEKKGSKLFGLPVGRPRSMSENLRKGIAATPQLFFYSISERLFKFPLSDHLCATFFDVLLGGASPKQVLQKRSQSNNELKDRSSTSVVSLAPFYVPQILVCIFKYMHSCQDTSARTKILSDLLDLLDSNPSNVEAIMEYGWSSWLETSVKLDVFRDYKSISKANDASLETNELILVRNMYSLVLSYFLCYVKGGWHQLEDTTNFLLLKIEEGQLTNFCLLRDIFEDIIGSLLETSPDENIFISQPCRDNILYLLKLSDELLVDQTGIKLLFCPPDMYAQSSSDGSPKDDISSEFVEIINAESDGQPASLPWSSNIFTDGEKLSDDWWSFYDKIWTLVWNLNGKGQNRSTPKGSNASVPSIGQRARGLVESLNIPAAEMAAVVVTGGIGTALSSKTNKVTDKAMMLRGERFPRIIFHLVIMYLCKAGLENASKCVQQFITMLPNLISEEDQCKNRLHFIIWSLLRVRSQYGELDDGARFHVISHLILETVIYGKSMLATSMLGKDDSAEANSNKESGFILNLVQKDRVLAAASDEVKYMKDAKADRLRQLQDLNSKLDERLIEDIEQLQSLEDDIQFAKTAAISGDDNRKAAFKLAFDEDQQIVADKWIHIFRALSDERGPWSASPFPNNLVTYWKHDKTEDKWRRRMKLKRNYKFDERLCQPLSTKSSNENAAPSVDPFVSAKIPEKMKRFLLKGVRGITGDVDSESCEDNNDTSEPSQNKPSENQVSSDAVNSADSSDYPTSVQNKKEQSSTGGDNDYAEVLSSVHCVLVTPKRKLAGQLTITRSALHFSFEFLVEGTGGSSVFNKFQDKKESDAKSELGSMEKLKGNSDVGRGNEAGSGDALMKSQSNKIKHHRRWKITRIRAVYWTRYLLQYTATEIFFDDANAPIFLNFSSQNDAKNVGSLLVSLRNDALFPKGSTKDKNSVISFVDRKVALEMAESARESWRKREISNFEYLMILNTLAGRSYNDLTQYPIFPWILADYSSEKLDFNKSSTFRDLSKPIGALDAKRFKVFEDRYLNFCDPDIPSFYYGSHYSSMGIVLYYLLRLEPFTALHRNLQGGKFDHADRLFQSIESTYRNCLSNTSDVKELTPEFFYMPEFLENLNSYHFGVKQDGEPLGHVGLPPWAKGSPEEFIHINREALESEYVSSNLHHWIDLIFGYKQRGKPAVEAANIFYYLTYEGAVDLENMDDMLQKSAIEDQIANFGQTPIQIFRKKHPRRGPPIPIAHPLYFAPQSITVTSIVPSAISSSSSVLFIGLLDSNIVLMSEGLILSVKLWLTTQLQSGGNFTFSGSLEPFFGIGSDVISPRKVATSLAENVEFGRQCVAAVQIQGDNYLILCGNWENSFQIISLSDGRIVQSIRQHKDVVSCVAVSSDGSVIATGSYDTTIMIWHAFRGRSNDKRSKNASNDLSTKDHVIIENPYHILCGHDDIITCLFVSTELDIVISGSKDGTCIFHTLREGTYVRSIRHPSGAGLSKLVASQHGRLVFYSDSDLSLHMYSINGKHIASSESNGRLNCMELSRCGEFMVCAGDHGQIVLRSMHSLGVVWRYDGSGKTITSLAVTPEECFLAGTKDGSLIVFSIENPLLRKGGIQRNKVKSSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287TWP9</accession>
    <name>A0A287TWP9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123403465</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953339.1">
        <property type="nucleotide sequence ID" value="XM_045097404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287TWP9"/>
    <dbReference type="SMR" id="A0A287TWP9"/>
    <dbReference type="FunCoup" id="A0A287TWP9">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287TWP9"/>
    <dbReference type="PaxDb" id="4513-MLOC_37243.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0575340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0575340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0575340"/>
    </dbReference>
    <dbReference type="GeneID" id="123403465"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0477060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0477060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0477060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0575340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0575340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0575340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403465"/>
    <dbReference type="eggNOG" id="ENOG502R8FD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287TWP9"/>
    <dbReference type="OMA" id="PHWKGIC"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287TWP9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="29"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="135"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="10">
        <location>
            <begin position="629"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0575340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="79272" checksum="19782B20976B6F94" modified="2017-11-22" version="1">MDMRRAFACTLLLAALLPLSEADASSKLYIVYMGEKKHDDPSMVTASHHDVLTSVFGSKDEAMKSMVYSYRHGFSGFAAMLTESQAGTLAKCSHILSVRPNVYHESHTTRSWDFLGLDYDQPPEHSGLLQKAKYGEDVIIGVIDSGIWPESRSFDDSGYGPVPARWRGTCQTGQQFDATSCNRKIIGARWFSGGMSDEVLKGDYMSPRDLSGHGTHVASTIAGEQVRNVSYGGLAAGVARGGAPRARLAIYKALWGQRGSGSHAGVLAALDHAIDDGVDVLSLSLGQAGSELFETLHVVERGISVVFSAGNGGPVPQTAWNAVPWVTTVAASTIDRSFPTLISLGNKRKLVGQSLHNNAYVNTDDFKILVYARSCNTQSLASRNITGKIVLCYAPAEAAITPPRLALPIVINRTMEVDAKGLIFAQYDTNILDILTMCKGNMACVVVDFETAHTILAYFDNSKKPVVKVSPAMTVTGNQVLSPMIASFSSRGPSAAFPGILKPDVAAPGVSILAAKGNSYVFMSGTSMACPHVSAVVALLKSAHSDWSPAMIKSAIMTTASVTDHFGVLIQAEGVPRKLADPFDFGGGHMDPDRAIDPGLVYDMNAKDYNKFLNCIDELSDDCKSYISNLNLPSITMPDLSDNITVRRTVMNVGQVKATYRVVVEAPAGVVVTVEPSMISFIEGGSKSVMFMVTFTSRKRVQGGYTFGSLTWSDENTHSVRIPIAVRTIVQDFVTDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W8F2</accession>
    <name>A0A8I6W8F2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123451100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group III family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984048.1">
        <property type="nucleotide sequence ID" value="XM_045128113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W8F2"/>
    <dbReference type="SMR" id="A0A8I6W8F2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090470"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090480"/>
    </dbReference>
    <dbReference type="GeneID" id="123451100"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0074430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0074430.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0074430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123420228"/>
    <dbReference type="KEGG" id="hvg:123451100"/>
    <dbReference type="OMA" id="DELFLHM"/>
    <dbReference type="OrthoDB" id="1888929at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010193">
        <property type="term" value="P:response to ozone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000009">
        <property type="entry name" value="WRKY transcription factor 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF139">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="91"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090470.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="247" mass="25790" checksum="8189078673AD2E0A" modified="2022-05-25" version="1">MEDAAATLATELDGLLAMARELEARVDGDQGAPGAARELCSALAASVDRAVRLAGRGRNAGARAGVNGQLRSGRKAAAAKVRTQVRVASMQDLGPLDDGMSWRKYGQKGILGATYPRSYFRCTHRHTQGCAATKQVQRATADPLLFDVVYVGAHTCAGAAVLGGTEQQLPPPAAFGQEQQSPPAAAPEGVQLPAEPVTPFSFPSSPASSWCQLTGSYGYAAPGGGLGVDMELEGQLDELFLHMSEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJS1</accession>
    <name>A0A8I6WJS1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJS1"/>
    <dbReference type="SMR" id="A0A8I6WJS1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF73">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="33"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="19087" checksum="44AA4C7B76ECCF63" modified="2022-05-25" version="1">MEWSTIHTHQPRNTTPTLVARWNPPEQGWLKANSDGALAKAHNRGGGGVVLRDHEGAYRGGACYVFRDVSHPEMAELLACREAVQLAIQTGAPKVHVEVDSIAVAAMVNERAKNLSLAGPIVEEIKLLGRTLEGFKVSWIRRTGNKGAHLLAKEGISRNNSSFWPNDPLVVFFM</sequence>
</entry>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        </source>
    </reference>
    <reference evidence="1" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0013910.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.1HG0013910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0013910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25912" checksum="A3279303EE1C64FA" modified="2022-05-25" version="1">MSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMTVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDAHALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTRLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
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        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044965697.1">
        <property type="nucleotide sequence ID" value="XM_045109762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WN05"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0143800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0143800.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0118450.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
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        <property type="entry name" value="VTT_dom"/>
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    </dbReference>
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        <property type="entry name" value="OS04G0592600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="33"/>
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        <location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
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            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0143800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="254" mass="27686" checksum="F352D78F0EBBFE29" modified="2022-05-25" version="1">MGREDRFPLLEAALGAGVAAVFTAGLVGVYLSMPDSDYSFLKLPRNLHELQILTGHVENYTSDYTVQVLIGYCVVYIFMQTFMIPGTIFMSLLAGALFGQLQGVALVVFAASAGASSCYFLSKLIGKPLVFVLWPDKLMFFRKQVAKRREKLLNYMLFLRVTPTLPNTFINLASHIVNVPFHTFLLATLIGLIPAAYVTVRAGIALGELTSLSDLYDTQSVALLFLIGVVSVTPALPRKDEVQEKAPEIAAGAS</sequence>
</entry>
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    <accession>A0A8I6WPK0</accession>
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        <recommendedName>
            <fullName evidence="4">Knottins-like domain-containing protein</fullName>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
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    <reference evidence="5" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953676.1">
        <property type="nucleotide sequence ID" value="XM_045097741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WPK0"/>
    <dbReference type="SMR" id="A0A8I6WPK0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0002530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0002530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0002530"/>
    </dbReference>
    <dbReference type="GeneID" id="123403821"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0001990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0001990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0001990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0002530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0002530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0002530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403821"/>
    <dbReference type="OMA" id="CHSERFS"/>
    <dbReference type="OrthoDB" id="1480833at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.10">
        <property type="entry name" value="Knottin, scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008176">
        <property type="entry name" value="Defensin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003614">
        <property type="entry name" value="Knottins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036574">
        <property type="entry name" value="Scorpion_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147:SF31">
        <property type="entry name" value="KNOTTIN SCORPION TOXIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00304">
        <property type="entry name" value="Gamma-thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00288">
        <property type="entry name" value="PUROTHIONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00505">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57095">
        <property type="entry name" value="Scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00940">
        <property type="entry name" value="GAMMA_THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035215850" description="Knottins-like domain-containing protein" evidence="3">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Knottins-like" evidence="4">
        <location>
            <begin position="37"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0002530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="84" mass="9202" checksum="B9C1672654FF5C06" modified="2022-05-25" version="1">MALLSPRRMAATPAALLLLLLLLVAATEMGKTAEARTCESQSHKFKGACFSDTNCASVCRTEDFPRGHCSTHYAARKCYCERDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQ55</accession>
    <name>A0A8I6WQ55_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQ55"/>
    <dbReference type="SMR" id="A0A8I6WQ55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0009470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0009470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0009470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0009470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0009470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0009470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="45"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0009470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="216" mass="25205" checksum="7AB7512C527311B8" modified="2022-05-25" version="1">MPTTFHEVVAHGTTKLRVVYTNLSTEVPFFLQQLKERWFNHAPDHEKFFGLDLEYTADQRGVAVIQICFASHVLIFQWARSDKHCPGLMEFLRSGVTFASVDIRNDRMKMRHSFGIEIPVSCLVDLQTIFTLRHDRTSMAHMAVALIDESYGDMKTSFPKYQHKLWEKSPLDDINIQYAAKDAYVSYELYRKIRVVNYGQRHLEERGHSDLDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUD8</accession>
    <name>A0A8I6WUD8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUD8"/>
    <dbReference type="SMR" id="A0A8I6WUD8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0159130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0159130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0159130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0131160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0131160.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0131160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0159130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0159130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0159130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000039">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.30:FF:000003">
        <property type="entry name" value="Os04g0303100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF30">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035222641" description="Receptor-like serine/threonine-protein kinase" evidence="22">
        <location>
            <begin position="24"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="53"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="369"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="528"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0159130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="839" mass="92675" checksum="257B6F8FD3098B23" modified="2022-05-25" version="1">MGPAGLHRFLLIVLAAAAPLLSASQRFDYPAANLSTLWINNKAFLPHSVSYSDGSAVRAIVLHSPKTFYGPSFAAGFVCASPCDVFLFAVFIVYTNSGAKITMPTTGTPQVVWSANRARPVRENATLELSSDGNLVLCDADASLVWSSSSSGRSVAGMMITEVGNLVLLDQRNATVWQSFAHPTDALVPGQSLLEGMRLVASTSATNWTENQLYMTVLPDGLYAYVGSTPPQLYFSKLVNNNKTGKDPTKVTFMNGSLSISVQSKQPNESDTTIQLPAANSTQYMRLESDGHLRLYEWSNADAKWAVVSDVIKLFPDDCAFPTVCGEYGICTGGQCVCPLQNNSSSSYFKPVDERKPNLGCASMTPISCQEMEHHQLLTLTDVSYFDASHIVVNATKGEDCKQACLKNCSCSAVMFRYGQNDSDGTCLWVTRVFSLQSIQPEIVHYNSSAYLKVQLSPSTSASNANKKKVMLGATLGAITILVVLVIAVILYVRRKKKYQELDEELDFDQIPGMTARYSFENLRECTGDFSKKLGGGGFGTVFEGKIGEQEVAVKRLEGARQGKKEFLAEVETIGSIEHINLVRLIGFCAEKSERLLVYEYMPRGSLDRWIYYRHNNAPLDWCTRHRIILDIAKGLCYLHEECRRIIAHLDIKPQNILLDENFNAKVADFGLSKLIDRDQSKVMTMMRGTPGYLAPEWLTSQITEKVDVYSFGVVVMEVICGRKNIDISLPEESVQLIKLLQKKAEDNQLINLIDKHSEDMVLHQEEAVQLLKLAMWCLQNDSSTRPSMSSVVKVLEGSMNIETNIDCNLMNANPVMSVPHNQSTYSVPPEASILSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUM8</accession>
    <name>A0A8I6WUM8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123441748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973947.1">
        <property type="nucleotide sequence ID" value="XM_045118012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUM8"/>
    <dbReference type="SMR" id="A0A8I6WUM8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063430"/>
    </dbReference>
    <dbReference type="GeneID" id="123441748"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063430"/>
    </dbReference>
    <dbReference type="OMA" id="VEICSNH"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032451">
        <property type="term" value="F:demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044842">
        <property type="entry name" value="ALKBH9B/ALKBH10B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447:SF2">
        <property type="entry name" value="RNA DEMETHYLASE ALKBH10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="323"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="548" mass="59768" checksum="BC5E37C8CA95AAB3" modified="2022-05-25" version="1">MTTPAAGAPGSPVAAPDQVAARDAVIGWYRGEFAAANAVIDALCGHLAQIGGADYDAVFAALHRRRLNWFPVLHMQKFYSVADVAAELRRVSDARAAAAYSEEEAASTVIHEPMDELVVTVAAEPEPIPEAQPDVAVHPVDAGREQEADGEDSSGDSSERKAASTEDDAVHDGHNTDQGSQGEHSLPESYPICSDHDECMARPERIKIQKGFMAKESVKGHMVNVVKGLKIYEDVFTTMELMKVADFINEIRQAGRNGELSGETFIFFNKQIKGNKREIIQLGVPLFQHTTEETNCHIEPIPVVLQAVIDHLVLWRLIPESRKPNSVIINFFDEDEHSQPYFKPPHLDNPISTLLLSETSMAFGRSLVTDSNGNYKGPLTLSLKQGSLLVMRGNSADMARHVVCPSSNRRVSITFVRVRPSTPVDLSPLPSPTKAMTLWQPPPTMATAGMQKSPHGSNGAIIGYCPAPQAMMAPAWGMAVRAPVMMVAAPARTMMMAPSSNINKRMGRGGTGVFLPWTVGPKRYNKHLPPRIQKRRFSAMMSPIESQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WVI7</accession>
    <name>A0A8I6WVI7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxidase 1</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 24">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="17">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WVI7"/>
    <dbReference type="SMR" id="A0A8I6WVI7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0217960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0217960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="479"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="738"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0239060.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1327" mass="150164" checksum="0D55AC19613AF1E2" modified="2022-05-25" version="1">MSNVNLNFNAFLEKNKLKDDGSNYADWVRNVKLILEAAKKAYVLNAPLGDPPAPAAAQDVLNVWQARSDDYSLVRCGMLYSLETGLQRRFEQHGAYEMFQELKLVFQAHARVERYEVSDKFFSCKMEENSSVSEHILKMSGLHGRLTSLGVELPDDAIIDRILQSLPPIYKGFVLNYNMQGMEKTIPELYSMLKSAEVEIKKEHQVLMVNKTTSFKKGRGKKNFKKDGKAVAAPGKPDAGKKKKNGPKPETECFYCKGKGHWKRNCPKYLADKKAGNVKGIYDIHVIDVYLTSARSSSWVFGTGAVAHICNSKQELRNKRRLAKDEVTMRVGNGSKVDVITVGTLPLHLPSGLVLNLHYCYLVPALSMNIVSGSCLMRDGYSFKSENNGCSIYMSDMFYGHAPLVNGLFLMNLDRDVTHIHSVSTKRCKVDNHSPTYLWHCRLGHIGVKRMKKLHTDGLLESLDFESFDTCEPCLMGKMTKTPFSGIMERATDLLEIIHTDVCGPMNVEARGGYRYVLTLTDDLSRYGYIYLMKHKSETFEKFKEFQSEVENQRDRKIKCLRSDRGGEYLSHEFGTHLRKYGIVSQLTPPRTPQRNGVSERRNRTLLDMVRSMMSLTDLPLSFWGYALETTAFTLNRAPSKSVETTPYELWFGKKPKLSFLKVWGCDAYVKKLQPEKLEPKAEKCVFIGYPKETIGYTFSHRSEGKTFVAKNGSFLEKEFLSKEVSGRKVELDEVITPPLEQESNAAQEVVPVVPTPTEEEVNDDDHEASDQVTTEPRRSTRVRSAPEWYGNPVMEIMLLDNGEPSNYEEAMAGPDSNKWLEAMKSEIGSMYENKVWTLVDLPDERRAIENKWIFKKKTDANGNVTVYKARLVAKGFRQIQGVDYEETFSPVAKLKSVRIMLAIAAFYDYEIWQMDVKTAFLNGNLKEELYMMQPEGFIDPKGANKVCKLQRSIYGLVQASRSWNIRFNEVIKAFGFIQVHGEACLYKKVSGSSVAFLVLYVDDILLMANDIEMLESIKAYLNKSFSMKDLGEAAYILGIKIYRDRSRRLIGLSQSTYLDKILKKFNMENSKKGFLLVLQGMRLSKTQSPTTAADREKMSSVPYTSAVGSLMYAMLCTRPDINLAISLVGRYQSNPGAEHWTTVKNILKYLKRTKEMFLVYGGDEELVVKGYVDASFDTDPDDSKSQTGYVYVLNGGVVSWCSSKQEVVAASTCETEYIAASEAAHEGIWMKELITDLGVVPSASGPMTLFCDNTGAIAIAKEPRFHRKTKHIKRRYNSIQDHVQTGVIDICKVHTDLNIADPLTKPLPRAKHDQHHDAMGVRYITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXH4</accession>
    <name>A0A8I6WXH4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXH4"/>
    <dbReference type="SMR" id="A0A8I6WXH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0328900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0328900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0328900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0328900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0328900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0328900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121">
        <property type="entry name" value="NO MECHANORECEPTOR POTENTIAL C, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121:SF25">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0328900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="265" mass="28289" checksum="9CA99A4C4995316C" modified="2022-05-25" version="1">MASMDPLLLAAAFSGSPAALRVLLDGEGSPVDVRADRVPTPAFLTLLKHGGAEEEDAAVDIASLQAFNTVLSPSLLEGVTAEGNTALHVVATNGDCARYLQSATFICRKARHLLHKANSNGDTPLHCAARAGRPFMVSRLIDLAKSGDVDDETAAGERLTKFLRRENGCSETALHEAVRIESNYIVELLMAADSELACFPSHDGASPLYLAVLHDRVDIAQTLYQKSGGCLSYFGPDGQNALHAAALRSQEPQICDECCFNILLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZV2</accession>
    <name>A0A8I6WZV2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Esterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123439623</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972249.1">
        <property type="nucleotide sequence ID" value="XM_045116314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZV2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245420"/>
    </dbReference>
    <dbReference type="GeneID" id="123439623"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0203730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0203730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0203730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0245420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0245420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0245420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439623"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF286">
        <property type="entry name" value="OS01G0214600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0245420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="40995" checksum="95D1A4EBEDE816BF" modified="2022-05-25" version="1">MHKIAKVLAISCNLAMLTKAVVPILFLLTVSRCETSQSYDAIYNFGDSISDTGNLCTGGCPSWLTMGQPPYGTSYFGRPTGRCSDGRVLVDFLAQFFGLPLLPPSRTNGTDFRKGANMAIIGATTMNLDFFDSHGLGSSIWNNGPLDTQIQWFQQLMPSICGGASDCMSHLSKSLFILGEFGGNDYNAPIFGGKSLDEIYTYVPHVINKITSGVETLIGLGAVDVVVPGVLPIGCFPLYLTLYGSSNQSDYDGDGCLQRFNDLSRYHNQLLKQGICSLQSKYAGVRLMYADFYTQVTDMLRSPQSFGLAHGLNVCCGASGQGSYNYNNEARCGMPGSSACKDPENYLNWDGIHLTEAAYRSIAYGWLTGPYCVPAILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0N8</accession>
    <name>A0A8I6X0N8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </evidence>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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    <sequence length="309" mass="33640" checksum="A518AAB9ECCDDE6E" modified="2022-05-25" version="1">MDGIYTRIHLSETLLRLYTLVGQIKKVIMAPPWVPSVAAIANSSAEDMEEPLDCGICFLPLHAPPVFMCEAGHLICSPCRGMLGLAVGMCHVCSAKAARPGSRRDDGRDVWPVYYAPRDRIGALRGPCRCPDNACGFVGNTAALLCHFVSAHDWPVHAGVTSGDSIAVRLQDGINIVAVDCIGGASEQDSPEFPGRYLLVLEVFQQTFGRTVFAFCLRPGAATMDGLIFWAKEAQCHLSLSYSRNILDGDDDKPLVQCYYQTTKASVTCTDLSSGLPDAEDCFQAHVYRSVDLEDNEDTVEVTLRVVIH</sequence>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <strain evidence="7">subsp. vulgare</strain>
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        <property type="entry name" value="TRYPSIN/FACTOR XIIA INHIBITOR-RELATED"/>
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        <property type="entry name" value="TRYPSIN_ALPHA-AMYLASE INHIBITOR CMX1_CMX3"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
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        <location>
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            <end position="149"/>
        </location>
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    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="137"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007107"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="16290" checksum="C61A890CBE9E536F" modified="2022-05-25" version="1">MAFKYQLILSAAVMLAILVATATSFGDSCAPGDALPHNPLRACRTYVVSQICHQGPRLLTSDMKRRCCDELSAIPAYCRCEALRIIMQGVVTWQGAFEGAYFKDTPNCPRERQTSYAANLVTPQECNLWTIHGSPSCPELQPGYGVVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3L9</accession>
    <name>A0A8I6X3L9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123426507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044966281.1">
        <property type="nucleotide sequence ID" value="XM_045110346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3L9"/>
    <dbReference type="SMR" id="A0A8I6X3L9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0161390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0161390"/>
    </dbReference>
    <dbReference type="GeneID" id="123426507"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0133140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0133140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0133140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0161390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0161390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0161390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426507"/>
    <dbReference type="OrthoDB" id="1927690at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36037">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE)-RELATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36037:SF1">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE)-RELATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0161390.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="412" mass="46127" checksum="323D17FCB064A861" modified="2022-05-25" version="1">MAETLALAPVEDPEAPLDAAAIRSRFEQLCTLWGGDKGELVEAAAAEDELRGLHSGCEKDMQAGDAWDSNAAELGGHGLDSYMEWLRKEVSLIEEENCKLSTEISVIGETVLKDTILLDAVIESLESRLNTLDSEVLKHSEVSPTSTDSVLNQISIEKVCEYEEFELDHQIGKSAMDLKLLQIQSISMQRDEEMWQLQSLFSEPRVLKCKGNCLRVFLKAPILPSECVNFGQKLDCVDHELLIEVDEGKIELNKVQIFPADVCVDILIEKLKSSREAISVPSLGWLIRQCQQQIIINTLRRLLVNDANNSRHSFEYFDKDETIVAHMVGAIDAFFKISENWPVSPYGLKLISIRNSGTQPTNITVELVCKTKELANGLELETRRQLVRFVDAVEEILVRGMQSELHSSRVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X482</accession>
    <name>A0A8I6X482_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123441905</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974018.1">
        <property type="nucleotide sequence ID" value="XM_045118083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X482"/>
    <dbReference type="SMR" id="A0A8I6X482"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063750"/>
    </dbReference>
    <dbReference type="GeneID" id="123441905"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0050750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0050750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0050750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0063750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0063750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0063750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441905"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000051">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="158"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0063750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="469" mass="49967" checksum="9E53E1DAF24B47DC" modified="2022-05-25" version="1">MAGVSAFSPSPPAPSPLAAADVAADALLAPWPLSPWPAPLRRGGDDGRSNPLFAILPLSALAIGLVLLVAVAVILVLTRRAKLRLVAAGDSVDGDKPGAPASSCGSSVRGYQNGRCYAATGCIYGGRLGPLGLAVVQPRSRGAQVFTYRELERATDGFGEGNVLGRGAYGVVFRGRLGDGTPAAVKRLQLDPRRQGEREFRVQVDLLSRMHSPHLVGLLGYCADQSHRLLVFEFMPNGSLKGHLHPAAGVDARPALDWQTRLGIALDCARALEFLHEHTSPAVIHRDFNCSNVLLDHNYRARVSDFGTAKVGSNKANGQVVTRVLGTTGYLAPEYASTGKLTTKSDVYSYGVVLLELLTGRVPVDTQRPPGQHVLVSWALPRLTNRERLVQMVDPALKGQFAVKDLIQVAAIAAMCIQTKAEYRPLMTDVVQSLIPIVKKSPAMSCSSTPARPLQHVVYMSPAAGSNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X5Z7</accession>
    <name>A0A8I6X5Z7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123443203</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044975440.1">
        <property type="nucleotide sequence ID" value="XM_045119505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X5Z7"/>
    <dbReference type="SMR" id="A0A8I6X5Z7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247420"/>
    </dbReference>
    <dbReference type="GeneID" id="123443203"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0205450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0205450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0205450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0247420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0247420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0247420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443203"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF6">
        <property type="entry name" value="OS01G0223600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
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        <property type="entry name" value="L domain-like"/>
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    </dbReference>
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    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035315580" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="22"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="335"/>
            <end position="606"/>
        </location>
    </feature>
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            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="605"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0247420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="637" mass="68121" checksum="5D64B458BF7D6BA8" modified="2022-05-25" version="1">MPPRLLTLAALLLLSAMLGAPEPDADRAALLDFLAGVGGGRAGRINWSATRPVCANWTGVTCSADGSRVVELRLPGLALTGPVPRRTLARLTALRVLSLRANSLSGAFPEDLLALPGLAGLHLQRNAFSGALPSGIAGLKTLQVLDLSFNGFNGTLPWGLSNLTQLVALNLSNNSLSGRVPDLGLPALQFLNLSNNHLDGPVPRSFLRFSDASFAGNSMTRSAPLSPAVPPPSLAPPAAGAPAKKRARLSEAVVLAIIVGGCVMLFAVVAVLLIAFCNRRGGSEDGSRTLSGKGGDKKGRESPESKAVTGKAGDGNRLVFFEGPSLAFDLEDLLHASAEVLGKGAFGTAYRALLEDATTVVVKRLKEVSAGRRDFEQQMELIGRIRHDNVAELRAYYYSKDEKLLVYDYYSRGSVSNMLHGKRGLDRTPLDWETRVRIALGAARGVSHIHTENNGRFVHGNIKASNVFLNSQQYGCIADLGLAPLMNPITARSRSLGYCAPEVTDTRKSTQSSDVYSFGVFVLELLTGKSPVQVTGGGNEVVHLVRWVQSVVREEWTAEVFDGELMRYPNIEEEMVEMLQIAMACVSRNPERRPKMVDMVKMIEEVGRNDSGTRASTEASTPVGEARSKAESSSAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X919</accession>
    <name>A0A8I6X919_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123447245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0335310"/>
    </dbReference>
    <dbReference type="GeneID" id="123447245"/>
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    </dbReference>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0335310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="38929" checksum="52924F26490A2A3E" modified="2022-05-25" version="1">MVAISMYRGNLHQSGDDAARRWPVPQPTLSASQFRRLLHRRSLAVSRLAGAPRPNSPNSRLDDGAAAVGEAREVDGGQQHHQADRHDVQMPPPPQQQQQGEEEGQDEQQQQGEGEGQDEQQQGEEEEEQQEEGAVEDVEMDDAGEVVAGDVDAGGNGDPEEGQGESEGFDPNSEVGQPDGVEERKRELTDKLDTLSKKKHDLVQMLKQILNAEEEIRTRSMQASLRAAMPQPPENTADGSVPRQVPRMTVDVNFSEFAGESDAGSNQGTPGRPLHHVHSISPSTASFARSPFGSRNHNPGNTPRSPAAPFSTVSPSRFAGTGHQGHHPSASLPASNFVASSPSPAASGGSSSVFRDYRPPSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBZ6</accession>
    <name>A0A8I6XBZ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger AN1 and C2H2 domain-containing stress-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123425638</name>
    </gene>
    <organism evidence="10 11">
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        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965270.1">
        <property type="nucleotide sequence ID" value="XM_045109335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBZ6"/>
    <dbReference type="SMR" id="A0A8I6XBZ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0133360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0133360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0133360"/>
    </dbReference>
    <dbReference type="GeneID" id="123425638"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0109940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0109940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0109940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0133360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0133360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0133360"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425638"/>
    <dbReference type="OrthoDB" id="431929at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF27">
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    </dbReference>
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        <property type="entry name" value="AN1-like Zinc finger"/>
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        <location>
            <begin position="7"/>
            <end position="55"/>
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            <begin position="95"/>
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        <location>
            <begin position="260"/>
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            <dbReference type="ARBA" id="ARBA00003732"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0133360.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="31503" checksum="3A490A4730B2F2AC" modified="2022-05-25" version="1">MGTPEFPDLGRHCSVGDCHLIDFLPFTCDRCDFVFCLQHRSYTSHKCPKANNKDVTVVVCPLCAKGVRLNPNEDANITWESHVNTDCDPSNYQRATKKKKCPVPGCREALTFSNTIMCKDCSKEHCLKHRFGPDHKCPGPRKPEPTFPFSNMLRRSQKVEPRANSSSSSSSWWSSSLLNAASSFRSSAEAGMQKLSIATSEAVQKAKDGVTPSSSGSGSGLVEQCVQCPARFSTVGALIEHAEKSHGNNSQPSRGKVTVDACPKCSKGFQDPVLLVEHVEREHGGTSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XC47</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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            <taxon>Triticodae</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0241610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="13371" checksum="9A8FDF921E617C62" modified="2022-05-25" version="1">MAKLSTGFERIHIQIEEQAHATAAFQQRNSELSHQVNDLQDQLQAERANTQEIINLERAEREQREGKLKEERSERERLLEAERTSRLKFEKKHDGKVCRIQQTDGNPTGVYKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6XEE1_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEE1"/>
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205880.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-norcoclaurine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF19">
        <property type="entry name" value="BET V I_MAJOR LATEX PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0017">Alkaloid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="4">
        <location>
            <begin position="28"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="182" mass="20157" checksum="433C6C6D6BACEAE5" modified="2022-05-25" version="1">MPLITSQLLGLQQRERAMEGGSLWHEFETDLPAADVWEVYGSLALGQLVPQLLPHVLSKVELVEGDGGVGTVLLVTFPPAGASEEPRSYKEKFNVVDHEKYIKEAEIVEGGFLDLRFRKYLVRFEVVGKEDGRSVIRSTVEYEVDAEHAKNASLVSTEALAAVAEAITKHIKEQKSPKQAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG90</accession>
    <name>A0A8I6XG90_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG90"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0486050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0486050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0486050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0402520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0402520.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0402520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0486050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0486050.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0486050"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0486050.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="97" mass="11586" checksum="52A3EC7A8BE1250A" modified="2022-05-25" version="1">MVLHITLVDTLQAGWQMYICSHSTIQNRTRQLQFRSCDVNDASSISVFWQSVRCLLIRSTPSTACSHRVASLSLLHWESYQWQREMLWFPFYLFQIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XG95</accession>
    <name>A0A8I6XG95_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein Sec24-like CEF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447882</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XG95"/>
    <dbReference type="SMR" id="A0A8I6XG95"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0288510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0288510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0288510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="6">
        <location>
            <begin position="482"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="7">
        <location>
            <begin position="557"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="9">
        <location>
            <begin position="802"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="8">
        <location>
            <begin position="897"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="1020"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1147" mass="122961" checksum="86888631C32074EF" modified="2022-05-25" version="1">MASFASDVPTLPPFFLRDPSLIFSVRVKCFLLSGRSRREESRCTQTGVFSPDPDLRYRLFSPSSPPPSPPMSRPPPPFTPQNPSPAPNPGGPASTLPASFSNLQISRAPPPSASAGPPPGDGPLPPSIRGPQAPPPGARPFPGSPPPPLQPGSLYARPAAPVQQSPPFGGPPAASVHQSPPFGGPPAAQPFQPQQRSPFNGPSSVLPAQVQRAPFGAPPGPSQARPFGGPPAAVSQPAPFGGSSVAPAQSAPYSAAPPPPFGGPPGAVPPPAYSGGPIPPFGAAPGPLQQGPYGGPPQYGGQRPGLQPPPFGAQTAPASQPPPFMGVPGANAPAFGPPGWQGQARPGAVRMPGGMLPNAPGHGMPSTPTMPYSPHAGAQVSTPSNIDPNQIPRPIAETSVVIFETRQGGQAAVPPAASSEFIVKDTGNCSPRLMRCTMNQIPCTGDLLTTAAMPLALMVQPFALPHPSEEAIQLVDFGEMGPVRCSRCKAYINPFMRFVDQGKIFICNLCGFSNDTPREYLCNLGPDGRRRDADDRPELCRGTVEFIATKEFLVREPMPAVYFFLVDVSMNAVQTGVTAASCSAISQVLSDLPEGPRTMVGIATFDSTIHFYSLKNARQQPLMFIVPDIQDVYTPLQMDLILPVSECRDSLEQLLETIPSMFENNRVADSAFGAAMKAGFLAMKPTGGKLLVFQSVLPSVGTGSLSARETEVRSNISTGDKEAHKLLQPVDKTLKTMALEFAEYQVCVDVFLATQSYTDIASISVVPSTTGGRVYYYFPFSALSDPAKIFNDLRWNVTRPQGFEAVMRVRCSQGLQVQDYSGNFCKRVPTDIDLPAIDSDKTIMVTFKHDDKFQENTECGFQCALLYTTVYGQRRIRVINISLPCTSTLNNLFRYADLEAQFTYVVKQAGIGIPSSSLSQVRDQVTSTCINILQAYRKHCASVSSSGQLILPEALKLLPLYTLALVKSIGLRNDGRVDDRSYWVSVVSSVSVLLAIPLVFPRMIALHDLTSRDDEDSLIPNPLTLNSENIQDDGIYLLENGEDGFIYVGNAVNPATLEQIFGLSSLAGAPNLLALEQFDNALSRKVNEVVNEIRRQRCSYLRLRLCRKGDPSGDFFRSLLVEDKAPGGLSYVEFLVHVHRQIQSKMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIP3</accession>
    <name>A0A8I6XIP3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123449906</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044983204.1">
        <property type="nucleotide sequence ID" value="XM_045127269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIP3"/>
    <dbReference type="SMR" id="A0A8I6XIP3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413390"/>
    </dbReference>
    <dbReference type="GeneID" id="123449906"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449906"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000018">
        <property type="entry name" value="2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF236">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="153"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="262" mass="28302" checksum="F624F5A09999901F" modified="2022-05-25" version="1">MLPPAPDRKRRPPPMEQTDAGDGAPQQRQRQKKSSAAKKAKKGGATGSGSGSGSGGAWPAIKPKKHLQVNRLKGTQLLTVPDFLTSAEAKAFVDVAESMGFTHQGSLGPLKGEAYRDNDRISVTDPLLAQTLWESGINRIFMDLNISGKVATGLNPNIRLYRYVEGQRFGRHIDESVNLGDGSRTQYTLLVYLSGKGSAKDSQALVGGETVFYDHRGGIVAEVAPVQGMALLHLHGARCMLHEARVVKKNVKYVLRSDVVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XK94</accession>
    <name>A0A8I6XK94_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XK94"/>
    <dbReference type="SMR" id="A0A8I6XK94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0381920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0381920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0381920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0381920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0381920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0381920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF50">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0381920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19623" checksum="B3A6B2E909D05AB7" modified="2022-05-25" version="1">MEQHTWAFTFGVICNIISLMVFLSPPPTFYRVYRKKSTEGFQLTSYLATLFSCLLWMYYVSLKSGSKLLLTINGVGYVIETLYIAMYLVYAPKSVRLLTAKLFIGLDVGLFGLIALVTMLASAGPLGVQVVGWICVAVALGVFAAPFNIIRLVIRTESVEFMPFSMSFLLVLSAVMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM22</accession>
    <name>A0A8I6XM22_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123443684</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976118.1">
        <property type="nucleotide sequence ID" value="XM_045120183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XM22"/>
    <dbReference type="SMR" id="A0A8I6XM22"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0264930.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0264930.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0264930"/>
    </dbReference>
    <dbReference type="GeneID" id="123443684"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0219690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0219690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0219690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0264930.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0264930.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0264930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443684"/>
    <dbReference type="OMA" id="MQSWQLA"/>
    <dbReference type="OrthoDB" id="1901658at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="282" mass="30650" checksum="DCF164784F3E3AED" modified="2022-05-25" version="1">MDGLRALIAAGATAVCCVVCAIWAFRSPSSSPPKKHIPSRCCGCASCGCHANTTFSSASGEMAVGGEMNKAPAPAPATTGASMMEQLVPEITTHALSYLDCTSLCRLSMTNSAMRRAANDDGAWKALYHKDFTAEQDTITPPNGWKAYYAATKAIMNVNAEFYNIIREGSLPAMSHFWLNSDYVKCVHATGELFTGYNAVMNSWGLLFNWGQEGGQGTDFQLRDVGARVLADVAWVNMKVHVDVDPGPFHVTNVYEFRNGRWYMVHHHSSLMADPAPHNLFG</sequence>
</entry>
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        </submittedName>
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    </gene>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="678" mass="72477" checksum="226E055BE5A1A20C" modified="2022-05-25" version="1">MESMRRHRPNHRFSKLNASRAQFTDADLDTSLLLQRLSVSPPCPNADAGAPLTPFHPPSPPRLPTAVVTAAMPRPSSKTPSTSQTRRVSPSPTPSPATAPRPKNPGAAAQRRRSPLSDLNSGDASAARAGCFRFLLSSASGSKPRCASTPRTPASATAPRAEAKRRAAGAGRLPDQESRTKAEQWPGREPRRRRDDPIGCQIRRVEPDKKQQGLARRQQQPQDLEALTPERKADAGSNPSSGATPPIHASISPEVLAGGAATPACFAAGHHVVPGVRDRRKCRARGILDIAGEGTSGELDAEPSRASIHWLSSPLGEAGTCSTKCGKEATVIWLSSPRDEGAACLFDEEIFLPRCSSEDPFWQHSPDCKGLLGSPVLGGLLDFDTPMSELSETTPSSGFLPVQRTPSTGDSISPFSLIVKRASSHSSRLSSLCAQRRLGSSYGYDSATDPTRISGESWSENGSTGNCSGLVRVSSRPLTRMDPVVECLEMMSLSPKPGDADYDQNDEDGALPATLPELSFQFAGASTPLESIDLSSFKRSPCDTELKGKEASFQKQASTEARISWREGLVSRIFDMGDLDCCQWLSDDEDGPVIPGNVEALPDTTLQPVTACCLQEDSKQSGFGSVEFGCGGGGLNYDSKATPNSVQVAESMRAEGFELVWSDDSDWTLLYKNGLFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="EnsemblPlants"/>
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        </source>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="103" mass="11228" checksum="9721CAEA26619897" modified="2022-05-25" version="1">MTMTLEDMAMISGLPLNGDTVTGHVSAVNWRQRVGVLIGVEPPPPLPGKPDASKVKHSWLKEVRGAPCPADADDIVVQQYAWAYLWYVLTKVVLTDTIDNSAL</sequence>
</entry>
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    <accession>A0A8I6XSF6</accession>
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        <recommendedName>
            <fullName evidence="9">GOST seven transmembrane domain-containing protein</fullName>
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    </protein>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044947951.1">
        <property type="nucleotide sequence ID" value="XM_045092016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSF6"/>
    <dbReference type="SMR" id="A0A8I6XSF6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480540"/>
    </dbReference>
    <dbReference type="GeneID" id="123397490"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0398250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0398250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0398250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397490"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
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    <dbReference type="GO" id="GO:0005794">
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        <location>
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            <end position="30"/>
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    <feature type="chain" id="PRO_5035228499" description="GOST seven transmembrane domain-containing protein" evidence="8">
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            <end position="532"/>
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            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="368"/>
            <end position="388"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="430"/>
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            <begin position="442"/>
            <end position="466"/>
        </location>
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        <location>
            <begin position="226"/>
            <end position="471"/>
        </location>
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        </location>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="532" mass="58856" checksum="CFE98840A2F050BB" modified="2022-05-25" version="1">MAADADTAAAARALLLLAVSVAALLRAGEASVHEYTGLGFLNKGNAFILHAGSEGLYAPVSPANATAEEEDDEAAAAAAPDAFIRFDKITFRRPEEANDSVKETSSSKVQILVFEIEDREMIGGSAYGGQKAICCTSDLAKLGACVEGSVIYRPSQVNPGWPQLFVASFDGSDLIATLPSRTIPVKKTGMYNMYFIHCDPALAGLEIDGKTIWKNPTGYLPGRMAPLKNFFGLMSFAFVILGIYWFYQYMKFWREVLPLQNCITLVITLGMLEMALWYFEYAEFNETGVRAKAITFWAVTFGTIKRTVARLIILIVSMGYGVVRPTLGGLTSKVVMLGGTFFVATEILELVENLGTVNDLSGKARLFLVYPVAILDASFIVWIFISLAKTLSQLQARRLMAKLDIYRKFTIALAITVLVSIGWIGYEIYFKSTDVFNERWQYAWIIPAFWHALSFSLLCVISYLWAPSQSSMRFTNDASEKFDREDSLSLIRPRPIVSKNGWSVTSAPDAKATNNTNTVASFDEDDEENKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU09</accession>
    <name>A0A8I6XU09_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123440759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="OS01G0930900 PROTEIN"/>
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    </dbReference>
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            <end position="197"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0312910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="273" mass="28429" checksum="EE64ED842A9A32B4" modified="2022-05-25" version="1">MAAAASREERWSLAGKTALVTGGTKGIGRAIVEELAGFGVRVHTCARSDADVQERVRGWAADAEAGSLRGRVTASACDVSVRGDREALMAAARAELGGKLDILVNNAGQTFFMPATESTAEDYARLMATNLESCFHLAQLAHPLLVAAGGGSVVNISSVAAAIAYPVLSVYSATKAAMNQLSRNLAVEWAGDGIRVNCVAPGGIRSDILRSSCIEVDVELLRGMAEKENARVALGRMGEPEEVASLVTFLCMPAASYVTGQVIYVDGGRTIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUV9</accession>
    <name>A0A8I6XUV9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUV9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0268740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0268740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0268740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0321940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0321940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0321940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16574">
        <property type="entry name" value="RING-HC_Topors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058746">
        <property type="entry name" value="Znf_RING-type_Topors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47361">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47361:SF4">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="36"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0321940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="272" mass="29493" checksum="F6A8607AAD074855" modified="2022-05-25" version="1">MAAAGSSAASLQAPLYLNDKAAAVGGDRAAGGCGVCAICLDKIPLQETALVKGCDHAYCVTCILRWASYKQTPVCPQCKHPFEFLSVHRSLDGCLHDYMFDESVCLLLRATWFKPLVVEAHDEAQEEEDIYRSYQYDDGDEDDLYEEYISRSPSIRIGNRRWGDNGYVRGGRREARPVVIPPAADAVPSRTPRKKEASASGSGSGSVSKDVAGRRARRAQKREAADKAAAEKHLKLLQRLGRGKTPEAPPEAPESLEVGPPESLEVGPPVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XW33</accession>
    <name>A0A8I6XW33_HORVV</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123396085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947057.1">
        <property type="nucleotide sequence ID" value="XM_045091122.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044947058.1">
        <property type="nucleotide sequence ID" value="XM_045091123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XW33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0420920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0420920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0420920"/>
    </dbReference>
    <dbReference type="GeneID" id="123396085"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0350390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0350390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0350390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0420920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0420920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0420920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396085"/>
    <dbReference type="OrthoDB" id="627955at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
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        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0420920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="190" mass="20104" checksum="982543E52EC6946E" modified="2022-05-25" version="1">MGNKCLVVVTSILAVLTVVFGVISAVLLALKHHQHDNGEFYTYRRSPAMPCGVVAAVLASMTQILASVAICCCGAWRVTKGGKRIAAVVFFITSWVLAIIAVLLFLAGAMLGFEGSAKKTVGNVRIVGGVALFVIATFLFLVVAALDVTSYRLVRKKDHYQAYVGSNPPMVPATSKDGPNAFAPAPHNQV</sequence>
</entry>
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    <accession>A0A8I6XWI0</accession>
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        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein mS41 SAM domain-containing protein</fullName>
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        <name evidence="2" type="primary">LOC123447462</name>
    </gene>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34955:SF2">
        <property type="entry name" value="IGR MOTIF PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="1">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0337220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="109" mass="12010" checksum="BED7A034B0EBB8ED" modified="2022-05-25" version="1">MAFACASHVRRLLLLHPGTGAPARSFYAQPYQAKVGVVEFLNGVGKGVEAHAAKLEEAVGGDLQRLLETRTLRLKKLGVPCKHRKLILSFAHKYRLGLWKPPAEARKAQ</sequence>
</entry>
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    <accession>A0A8I6Y4X6</accession>
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    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="9" type="primary">LOC123447487</name>
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    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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    </comment>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414810"/>
    </dbReference>
    <dbReference type="GeneID" id="123447487"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345520.1.CDS.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345620.1.CDS.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447483"/>
    <dbReference type="KEGG" id="hvg:123447487"/>
    <dbReference type="OrthoDB" id="644057at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF21">
        <property type="entry name" value="EXPANSIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158843" evidence="6">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="7">
        <location>
            <begin position="60"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="8">
        <location>
            <begin position="180"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="262" mass="28264" checksum="8CB38E80BD0015D5" modified="2022-05-25" version="1">MASSSALLVAAVLAVVVCGAHGIAKVPCGPNITATYVSEWKDAKTTWYGKPTGAGPKDNGGACGYKEVDKAPFFGMTSCGNVPIFKDGRGCGSCFELKCTKPEACSGEPTIVTITDKNEEPIAAYHFDLSGHAFGTMAKKGQEQKLRDAGEVEIKFRRVKCKYPEGTKVNFHVEKGSSPNYLALVVKFLQGDGDVVAVDIKPKGKDKWIELKESWGAVWRIDTPDKLIGPFSVRYTTEGGTKTVAEDVIPEGWKPDTSYETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7Q2</accession>
    <name>A0A8I6Y7Q2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123413596</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962468.1">
        <property type="nucleotide sequence ID" value="XM_045106533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7Q2"/>
    <dbReference type="SMR" id="A0A8I6Y7Q2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0639910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0639910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0639910"/>
    </dbReference>
    <dbReference type="GeneID" id="123413596"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0531720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0531720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0531720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0639910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0639910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0639910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413596"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000110">
        <property type="entry name" value="Argonaute 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000013">
        <property type="entry name" value="protein argonaute 10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024357">
        <property type="entry name" value="Argonaut_Gly-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12764">
        <property type="entry name" value="Gly-rich_Ago1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="PAZ" evidence="4">
        <location>
            <begin position="556"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="5">
        <location>
            <begin position="845"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1169"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0639910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1216" mass="133788" checksum="DCF0319592453494" modified="2022-05-25" version="1">MVRKKRTGTGESSGEASGAPGQGSSQPAERAPPQQHGGGGGRGWVPQQGGRGGGQHLGRGGGQYQGRGGPPEYQQRDYQGRGQAGGGLPEYQQRDYQGRGQTGGGPPEYQQRGPQGRGNSGGGPPEYQPRGPQGRGNPGGGPPEYQPRGPQGRGHQGGGPPEYQPHDPQGRGHPGGGPPEYQPRGPQGRGHPSGGPPEYQPRGPQGRGHPGGGPSEYQPRDPQGRGGPPEYQQHDYQGRGGPRPRGGGMPQPSYGGHRGGRGGHNVPPGQSRTVPELHQAPDVQYQAPVVSPSASGAGSSSQPVAEVSSGQVEQQFQKLDISDQSSTSQAIQPAPASSKSVRFPLRPGMGKCGDRCVVKANHFFAELPDKDLHQYDVTITPEVTSRGVNRAVIAELVKLYRQSHMNGRLPAYDGRKSLYTAGPLPFTTRTFEIALQDEDEGLVGGQATPRRERQFRVVIKYAARADLHHLAMFLAGRQPDAPQEALQVLDIVLRELPTARYSPVSRSFYSPNLGRRQRLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIEFVAQLLCRDISVRPLTDSDRVKIKKALRGVKVEVTHRGNMRRKYRISGLTSQATRELSFPVDDRGTVKTVVQYFLETYGFNIQHTTLPCLQVGNQQRPNYLPMEVCKIVEGQRYSKRLNEKQITALLKVTCQRPQEREKDILQTVHHNAYYEDPYAQEFGIKIDEQLASVEARVLPPPRLKYHDSGREKDVLPRVGQWNMMNKKMVNGGRVSHWACINFSRNVQDNAAKVFCHELAIMCQISGMNFAPEPVLPVLSARPEHVERALKARYHDAMNASNPPGKELDLLIVILPDNNGSLYGDLKRICETELGLVSQCCLTKHVFKMSKQYLANVALKINVKVGGRNTVLVDALARRIRLVTDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLVSAQAHRQELIQDLFKVWQDPQRGTVTGGMIKELLISFKRATGQKPQRIIFYRDGVSEGQFYQVLLFELDAIRKACASLEPNYQPPVTFVVVQKRHHTRLFANNHNDQRTVDRSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPDTSDSGSVASGARGGPPQGGPRSSTRFGNVAVRPLPALKENVKRVMFYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9D8</accession>
    <name>A0A8I6Y9D8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123446899</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979376.1">
        <property type="nucleotide sequence ID" value="XM_045123441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9D8"/>
    <dbReference type="SMR" id="A0A8I6Y9D8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332920"/>
    </dbReference>
    <dbReference type="GeneID" id="123446899"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446899"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="79"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="267" mass="30580" checksum="129412DE072C5B7B" modified="2022-05-25" version="1">MDPKLVEVTQLFERFKAACKRSDLDASATLLSQLKVLLTKFPSLPPLFQQTPNAVEELKLAREIYEHAVLLSVKMEDQDAFERDFCQLKPYYMDTCGILPPSSMEYPIMGLNLLRLLVQNRIAEFHTELELLPAKALENACIKHGVELEQSFMEGAYNRVLSARQTAPHETYVYFMDLLAKTVRDEIAGCSEKGYDYLSISDAKQMFMFSSDKELQQYIAEEHPEWDVKDGRVLFQKAKESQPCKEIPAAPVINQTLGYARELERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAI3</accession>
    <name>A0A8I6YAI3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123410033</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044958846.1">
        <property type="nucleotide sequence ID" value="XM_045102911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAI3"/>
    <dbReference type="SMR" id="A0A8I6YAI3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0664700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0664700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0664700"/>
    </dbReference>
    <dbReference type="GeneID" id="123410033"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0664700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0664700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0664700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410033"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000187">
        <property type="entry name" value="E3 ubiquitin-protein ligase ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF581">
        <property type="entry name" value="OS06G0218300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035228502" description="RING-type E3 ubiquitin transferase" evidence="17">
        <location>
            <begin position="29"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="347"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0664700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="416" mass="44749" checksum="6A247966830F567D" modified="2022-05-25" version="1">MAKPTPPSCRHAVLLAALVLAAGAVARADDDDERDRKSDGGRGVVYGARKPPSFSAPMVVLLVALIATFFFIGFFSIYIRQCGRGNSPTIPAAAFLVLSRQEQQQQARPRGLDPDLVASFPAMTYAEARALREKEAGGKGADGTAVLECAVCLSEFEDGDQLRLLPKCSHAFHPDCIGEWLAGHVTCPVCRCSLAPEEPAAPAAAEADGAGGELQRAPEVAIDMNREGDGEEQEERMREAAELERIGSMRRAVRSRSRSGRPFSRAHSTGHSLSARFDGDLERFTLRLPEHVRRDMVAAGEESLRRTVARDAGARSARIGRSDRWPSFIARTFSSGVPFWAASRRAPDADAPAQCPPTPAPTAPPLPRTVREKTPDGSVVGSANSKGSVRFDCLGGRVAGDSEEEPEEEKAIIRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAR5</accession>
    <name>A0A8I6YAR5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123413539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962408.1">
        <property type="nucleotide sequence ID" value="XM_045106473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAR5"/>
    <dbReference type="SMR" id="A0A8I6YAR5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667150"/>
    </dbReference>
    <dbReference type="GeneID" id="123413539"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0553530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0553530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0553530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0667150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667150"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413539"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04133">
        <property type="entry name" value="Rop_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000512">
        <property type="entry name" value="Rac-like GTP-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="23560" checksum="964C77112D127B8E" modified="2022-05-25" version="1">MASSASRFIKCVTVGDGAVGKTCMLICYTSNKFPTDYVPTVFDNFSANVVVDGTTVNLGLWDTAGQEDYNRLRPLSYRGADVFVLSFSLVSRASYENVMKKWLPELQHHAPGVPTVLVGTKLDLREDKQYLLDHPGVVPVTTAQGEELRKHIGATCYVECSSKTQQNVKAVFDAAIKVVIKPPTKQRERRKKKARQGCASLGTLSRRKLACFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBN2</accession>
    <name>A0A8I6YBN2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBN2"/>
    <dbReference type="SMR" id="A0A8I6YBN2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0504060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0504060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0504060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="32"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0504060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="215" mass="25048" checksum="B5B61C3318382D9A" modified="2022-05-25" version="1">MPTSFHEVVAHGTTKLRVVYTNLSTEVPFFLQQLKDWFNHAADHEKFFGLDLEYTANQRGVAVIQICFASHVLIFQWARSDKHCPGLMKFLRSGVTFASVDIRNDRLKMRQSFGIEIPAGCLVDLQTIFRLRHDRTSMAHMAIALIDESYDDMKTSFPKYQHKLWEKGPLDDINIQYAAKDAYVSYELYRKIRVVNYSQRHLEKRGHSDLDDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCG1</accession>
    <name>A0A8I6YCG1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCG1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0474070.1">
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0474070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0474070.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26516" checksum="70D91A4717149D34" modified="2022-05-25" version="1">MLVRVMSVCGYRWYPEYKVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYFDPSMEDPLLQSTVEMLENRHRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWLAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCM0</accession>
    <name>A0A8I6YCM0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCM0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0686440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0686440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0686440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0686440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0686440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0686440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF37">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0686440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="27507" checksum="62C7E95E924A7143" modified="2022-05-25" version="1">MDQVMDAPPSGTVESDYETIAGMRYKEMAASKGKPFPFKHAWAILQTFDKWKLRDQETAPKKSTMLRMDDSEDEEERNLGKPERTKKGKLRVKMEGEASSLREKMEHMMKPREELTTKTFETKLLITKKKKEVKLAQVEEKREEAKRKADLEERMIKLKEAKVWKELMVEEKEHMMMSKKDMDQDQLQWWKDYKEDIAERKRMFRGASSTFRGDTPMSSCGDGGVDDSTRAYGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YG55</accession>
    <name>A0A8I6YG55_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123407081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044956056.1">
        <property type="nucleotide sequence ID" value="XM_045100121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YG55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707750"/>
    </dbReference>
    <dbReference type="GeneID" id="123407081"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0587030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0587030.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0587030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0707750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0707750.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0707750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407081"/>
    <dbReference type="OrthoDB" id="1889094at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF61">
        <property type="entry name" value="OS01G0574800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="82"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0707750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="213" mass="23311" checksum="147894FCBD923E4D" modified="2022-05-25" version="1">MGKASTVSSWLCCPCRCLFCGLLSCLFSVVATILVIAGVVVLALYLLFRPHLIHATAASADLGNFTLTPQTWILCYNLSVALSVRNPNSRIAIHYHSVEAEAYYMGQSFARADLPDFYQETGETTVLPLAFAGDHPLEGGVAAAGFRKEAIDHATFSVDVKLSAKMQLKVWAFRVPGPKPRVDCPLIIQRRNASEPTGASPPEFHPIECRVWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGZ9</accession>
    <name>A0A8I6YGZ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123408553</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957569.1">
        <property type="nucleotide sequence ID" value="XM_045101634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGZ9"/>
    <dbReference type="SMR" id="A0A8I6YGZ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717390"/>
    </dbReference>
    <dbReference type="GeneID" id="123408553"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0595070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0595070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0595070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408553"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033907">
        <property type="term" value="F:beta-D-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Beta-glucosidase 12"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
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        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF302">
        <property type="entry name" value="BETA-GLUCOSIDASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035256284" description="4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase" evidence="7">
        <location>
            <begin position="30"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="59102" checksum="4900F8DC60EDBA8D" modified="2022-05-25" version="1">MACKWQCVATPVLLLVWLAASATQQGCSSAQTRRSGNDSLTRGSFPKGFVFGTATSAYQYEGAVKVDGRGPAIWDKFAHTFGKILDFSNADVAVDHYHRFEEDIQLMADMGLDAYRFSIAWSRILPNGTGEVNQAGIDHYNKVIDALLAKGIEPYVTLYHWDLPQALEDKYNGLLDRQIINDYTAYAETCFKAFGDRVKRWITFNEPHTVAVQGYDSGIHAPGRCSVLRHLCCKQGSSGTEPYIVAHNIILAHATVSDIYRKKYKAEQNGEVGMSLDVIWYEPVSNSTADVEAAKRAQEFQLGWFADPFFFGDYPATMRSRVRERLPRFMTKEADLVKGSLDFVGINHYTTFYTKEDHSTVIKYLLNDTLADSGSVSLPFRNGKPIGDKANSIWLYIVPGSMRRLMNYVKDRYNTPTVYITENGMDDSNSPFISLKKALNDTKRINYHNDYLTNLADSIREDGCDVRGYFAWSLLDNWEWTAGYTSRFGLFFVDYKDNLKRYPKNSVQWFKKFLAFPDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJK8</accession>
    <name>A0A8I6YJK8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123408764</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957742.1">
        <property type="nucleotide sequence ID" value="XM_045101807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YJK8"/>
    <dbReference type="SMR" id="A0A8I6YJK8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0744200.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0744200.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0744200"/>
    </dbReference>
    <dbReference type="GeneID" id="123408764"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0617060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0617060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0617060"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0744200.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0744200"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10056939at2759"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000143">
        <property type="entry name" value="homeobox protein knotted-1-like 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELK_dom"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KNOX1"/>
    </dbReference>
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        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM01188">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELK" evidence="9">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="221"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="222"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0744200.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="310" mass="34086" checksum="0CA4BBF2D38451A7" modified="2022-05-25" version="1">MAYQYHHHQDHALGMDAAAAAAAGGNPGGGGFAPGLSAGGAWEGEKAAVEAHPLYERLLEAHVACLRVATPVDQLPRIDAQIAARAPPPMPPAGALSGGEELDLFMTHYVLLLCSFKEQLQQHVRVHAMEAVMACWELEQTLQSLTGASPGEGTGATMSDDEDNPVDSESNMFDGNDVSDGMGFGMLTEGERSLVERVRQELKHELKQGYREKLVDIREEILRKRRAGKLPGDTASTLKAWWQAHAKWPYPTEEDKARLVQETGLQLKQINNWFINQRKRNWHSNPTSSSSDKSKRKRNNAGDGNAEQSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YPE0</accession>
    <name>A0A8I6YPE0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956724.1">
        <property type="nucleotide sequence ID" value="XM_045100789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YPE0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749600"/>
    </dbReference>
    <dbReference type="GeneID" id="123407622"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0621920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0621920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0621920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407622"/>
    <dbReference type="OrthoDB" id="1417760at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48237">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48237:SF1">
        <property type="entry name" value="SPC97_SPC98 FAMILY OF SPINDLE POLE BODY (SBP) COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="12935" checksum="7A261A45EFAD7C93" modified="2022-05-25" version="1">MSGSGRKYPSGSFKRKKKQQQDEEKEALSGSIFRYYKSKTSTSRNTEELEIVLGDDQNNVNREDDGHTPTEDDVDIDMDDTNVSDHDPIFNSSPAESSSVDEEPVITVDIYDPIN</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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            <strain evidence="3">cv. Morex</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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    <reference evidence="25" key="1">
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="25">cv. Morex</strain>
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    <reference evidence="24" key="2">
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate</text>
            <dbReference type="Rhea" id="RHEA:22780"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:57466"/>
            <dbReference type="EC" id="1.13.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z,12Z,15Z)-octadecatrienoate + O2 = (13S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoate</text>
            <dbReference type="Rhea" id="RHEA:34495"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:58757"/>
            <dbReference type="EC" id="1.13.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="20">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_044951381.1">
        <property type="nucleotide sequence ID" value="XM_045095446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0T5"/>
    <dbReference type="SMR" id="A0A8I6Z0T5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573420"/>
    </dbReference>
    <dbReference type="GeneID" id="123401613"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0573420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401613"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="UniPathway" id="UPA00382"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016165">
        <property type="term" value="F:linoleate 13S-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010597">
        <property type="term" value="P:green leaf volatile biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01751">
        <property type="entry name" value="PLAT_LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000021">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000005">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.372.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.375.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042057">
        <property type="entry name" value="Lipoxy_PLAT/LH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 19" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="15 20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="4 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 20" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PLAT" evidence="22">
        <location>
            <begin position="80"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="23">
        <location>
            <begin position="202"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="249"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="896" mass="101384" checksum="2492CEB9B908DBDA" modified="2022-05-25" version="1">MIHLKQPLVLSAQSSNVASPLFVAGGQQRRASGAGRTCSGRRLSARRISCASTEEAVGVSTSVTTKERALTVTAIVTAQVPTSVYVARGLDDIQDLFGKTLLLELVSSELDPKTGRERERVKGFAHMTLKEGTYEAKMSVPASFGPVGAVLVENEHHREMFIKDIKLITGGDESTAITFDVASWVHSKFDDPEPRAFFTVKSYLPSQTPPGIEALRKKELETLRGDGHSERKFHERVYDYDTYNDLGDPDKNIDHKRPVLGTKEHPYPRRCRTGRPKTLYDPETETRSSPVYVPRDEQFSDVKGRTFSATTLRSGLHAILPALAPLLNNSQCFSHFPAIDALYSDGIPLPVDGHGGNSFNVINDVIPRVVQMIEDTTEHVLRFEVPEMLERDRFSWFRDEEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKMMNGRMTVEEAMEKKRLFLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFLSDEGTLMPLAIELTRPQSPTKPQWKRAFTHGSDATESWLWKLAKAHVLTHDTGYHQLVSHWLRTHACVEPYIIATNRQLSRMHPVYRLLHPHFRYTMEINALAREALINADGIIEEAFWPGKYSIELSSVAYGAAWQFNTEALPEDLINRGLAVRRDDGELELAIKDYPYADDGLLIWGSIKQWASDYVDFYYKSDGDVAGDEELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAVNFGQYHYAGYFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEAEGTIDMRNNNPENKNRCGAGIVPYELLKPFSEPGVTGRGIPNSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z174</accession>
    <name>A0A8I6Z174_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123401002</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044950653.1">
        <property type="nucleotide sequence ID" value="XM_045094718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z174"/>
    <dbReference type="SMR" id="A0A8I6Z174"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579960"/>
    </dbReference>
    <dbReference type="GeneID" id="123401002"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0480960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0480960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0480960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0579960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0579960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0579960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401002"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010182">
        <property type="term" value="P:sugar mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000193">
        <property type="entry name" value="Serine/threonine-protein kinase CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF302">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIS8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="603"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0579960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="867" mass="93969" checksum="602B6CE897CA15BD" modified="2022-05-25" version="1">MSRMKHLLRKLHLSGAGAGNASAGGATAATPSPDHHRPRQRRSAHSPLPPPVVAAAAVAELPLPAPAAAAPEEPTGMGVETTMTRLEEEYHVRLALAISASDPAGLVDPDSVQMRAAERISLGGPAAAPGDRTTMEALSARYWNHNVVNYDEKLSDGFYDVCGAPMDPGFQVKFPSLSSLRAIPVGRDVAYVAILVNRERDPTLKRLEGTAIAIAAQSRAERGGIASAELVQKIASLVVDAMGGVVEDADAMNREWSTKSRQLCAGLNSIALPLGSLGIGLSRHRSLLFKVLADRVNLPCKLVKGICYTGTDEGAINFVKIDFDSAEYIVDLMGAPGTLIPSEISVSQFQDSNNSQLSSDAIEESVAELCIALEQVSGVYESKSDMGGSSSDRNSVLALRTPHLEDTCHTENPLKQHIISDEGQFDEFNIKGSVSQQNKVNDTSKYLVPGVVDPQFAQNLHDLLLEGGALLPSGLLSCQNSHNSGNTTEMGKNSSPEVKEIPGWMLVAQTGQNSPECSVAEDSLPKIALPPREDVQYPVENTEATIRSLDTISTEGERVAEDSLANMSGSSSANLDKLSCSSTKTISSVMDDVAEYEISWEDLHIGERIGLGSYGEVYHADWNGTEVAVKKFLDQDLSGVALEQFKCEVRIMSRLRHPNVVLFLGYVTQPPNLSILTEYLPRGSLYRLLHRPNSKVDETRRLKMALDVAKGMNYLHTSHPTIVHRDLKSPNLLVDKNWVVKVSDFGMSRLKHNTFLSSKSTAGTPEWMAPEVLRNEPANEMCDVYSFGVILWELATLCVPWSGLNPMQVVGAVGFQNKRLDIPKEVDPLVASIISSCWDNDPSKRPSFSQLLSPLKKLQRLLVTESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1G1</accession>
    <name>A0A8I6Z1G1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1G1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272">
        <property type="entry name" value="BED FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272:SF184">
        <property type="entry name" value="OS03G0311250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="81"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="21809" checksum="0415984D05612CEF" modified="2022-05-25" version="1">MNYIEWAFGELYGKETARFRNEMSDVKKQLEALYDKCVAQDKRAGNGASSSSTTTSIPSIPVQAGYQSFLSARSTRVSKSELRNYLEDEVAAADTCLDILNWWKINAPMYPIMAKIAKRFLAMPATSVSSESTFSTTGRILDAYRSSLKPVMVEALVCAASYIKGAHVDLNMVPRDENEEDDVETIKLPVVEEIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z2M7</accession>
    <name>A0A8I6Z2M7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123411244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044960113.1">
        <property type="nucleotide sequence ID" value="XM_045104178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z2M7"/>
    <dbReference type="SMR" id="A0A8I6Z2M7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0688880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0688880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0688880"/>
    </dbReference>
    <dbReference type="GeneID" id="123411244"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0688880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0688880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0688880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411244"/>
    <dbReference type="OrthoDB" id="10264753at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048440">
        <property type="term" value="P:carpel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:regulation of flower development"/>
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        <property type="term" value="P:regulation of root meristem growth"/>
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        <property type="term" value="P:sepal formation"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:stamen development"/>
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        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
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        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
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    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
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        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="11">
        <location>
            <begin position="492"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
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        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="649" mass="72804" checksum="84E14062D53E2748" modified="2022-05-25" version="1">MATTSESTANSTVTSATADGDTEREQEHGNGATAAPAVVQQEEEGEELIGPGPAPARQRQKRPLQFEQAFLDALPSAAMYEKSYMHRDVVTHVAVSSPADFFITGSADGHLKFWKKKPSGIEFAKHFRSHLSPIEGLAVSVDGLLCCTISNDQSVKIYDVVNYDMMFMMRLPFVPGAIEWVYRQGDVKPKLAVSDRNTSSVHIYDTHSGSNDPIISKEIHAGPVKVMKYNHAQDVVISADAKGLLEYWSPSTLKFPEDAVNFRLKTDTNLFELAKCKTSVSAIEVSNDGTQFVVTSPDRRIRVFWFKTGKLRRVYDESLEVAQDLQKSDVPLYHLDAIDFGRRMAVEKEIEKTENVPQPNAVFDESCNFLIYATLLGIKVVNLHTNKVPRILGKVENNERFLRIALYQGDKGNKKVRKIPSIAANVNDTKEPLSDPTLLCCAFKKHRIYTFSRREPEEPEDATKGRDVFNEKPPPEELLSLSELGKTATTSLPDNLVFHTSMGDIHLRLYPEECPKTVENFTTHCRNGYYDNLIFHRVIKGFMVQTGDPLGDGTGGQSIWGREFEDEFHKSLRHDRPFTLSMANAGPNTNGSQFFITTVATPWLDNKHTVFGRVVKGMDVVQQIEKVKTDKNDKPYQDVKILNVTVPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z5X2</accession>
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        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z5X2"/>
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    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0711100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="21378" checksum="F19950D4EFC2AB9E" modified="2022-05-25" version="1">MCMSPYKMVYGKACHLPLELEHKAYWAIKDLNFEFKLVGEKRLFDISSLDEWRTQAYENAKLFKEKVKRWHDKRIQKREFNVGDYVLLYNSRLRFFAGKLLSKWEGPYVVEEVYHSGAIKINNTEGNCPRVVNGQRIKHYILGTPINVEINIINTILRKNTQGIFISLFETPKMKRYVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B348</accession>
    <name>A0A8I7B348_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cellulose synthase-like protein H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123426726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044966536.1">
        <property type="nucleotide sequence ID" value="XM_045110601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B348"/>
    <dbReference type="SMR" id="A0A8I7B348"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0168310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0168310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0168310"/>
    </dbReference>
    <dbReference type="GeneID" id="123426726"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0138830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0138830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0138830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0168310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0168310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0168310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016759">
        <property type="term" value="F:cellulose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000176">
        <property type="entry name" value="Cellulose synthase-like protein B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <ligand>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </source>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="NAC domain-containing protein 100-like"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="15"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="357" mass="40463" checksum="DA34F1CD02DE56F4" modified="2022-05-25" version="1">MEDYLQVQQQQELHLPPGFRFHPTDVEIITSYLVPKVLKKPFDTRVVTEVDLNKHEPWELPNMAIMGEKEWYFFSEKDHKYPTGIRTNRAMTTGYWKATGKDKEIFQPSTMSLIGMKKTLVFYMGTTPKGEKTNWIMHEYRLKSGKQPSPDVPSDITNATIINTSSKEDYVVCRIFHKSTGLKKVVMSSYTMPMPMRVGAEHQHGFLESFTLPPLMDYNASSSLTPPLSLPAASSYQLQATRPAALMMDDTLLPMMNGDYIGNHHHQMLVAPPQPLIPFYHHHVEQQQHPMMQMQIMEMEMSADQGFMVGIEPGSEPSSIVSQEDVMIGLKNNDLGNGSTATPDEISSVNMGMDGMW</sequence>
</entry>
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        <name evidence="2" type="primary">LOC123443699</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46737:SF3">
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            <end position="83"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0265690.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="226" mass="25623" checksum="D929F299993CB8AD" modified="2022-05-25" version="1">MLPTLLGGYLTVSALPLPLPTATATPRTRILPGLRPSSRLVARRSAGETVHATATEEEEQEWKELQEEGLPRRGQHTEDDHDHDPEIADIMGDYFDDPKKAQSRMEERIKKKRHKIVQAKTGSPNPMNVVFNKFDFSNSYIWFEFHNALLPKDVKLICDALRSWHILGRLGGCNSMNMQLSQLSLDCKRPTYDALKAANATPTSFYNIGDLEIQENLARVWCGFFL</sequence>
</entry>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123429765"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33922:SF2">
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        <location>
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        </location>
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            <begin position="78"/>
            <end position="122"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0129330.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="264" mass="27627" checksum="BD088E50564F8E1F" modified="2022-05-25" version="1">MVDARHEPREAADSSTPDAADDFEFRVLSTGGLVSAGAGDMCVADELFSHGRLLPLRPSSSDALLPRSESVASTAGFGSRSDCRSASSSGSSSGCVSRSHSSKSASSDHAAPPPRRSLSSSLFYAHPSPSPQLRSRPRRSTGSAPPPAGWGFIRLGVVGAPDVYPPRCADGAKITAASRGGSGRSARFEQAASAIERSFRKHGAGLFGDRFGCKCSPDVVESVKLPESARKSGLADGNVKRGRGGRRIRILDWLEELSTTKAKK</sequence>
</entry>
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    <name>A0A8I7B7M5_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        <location>
            <begin position="104"/>
            <end position="348"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="47700" checksum="EB0EBFD4262DA911" modified="2022-05-25" version="1">MRPPERLGEIAATPQPPVSAELRDWGHLPDLVLTDVLDRLYPCLRSLSAFAAACRPWRRFFIASASTIVPRIPPLTLQARRNCGKSARLIGLSPAVIGDPVAVPLPAFRSTLLSASRGHLILLQRGYHGRCSNLVVVDAFTGAERLAAPLPNGFSYHYASLTPTHVLLFETYDHKFVSLRFPTTLNPDPQWTPHRLPRGASFLSTVIDFHGRVLGITDRAELLEFRLDEVNAPHHWQSHAVRLLPTTGLPDSDTATFERLRYGPRLVAAGERLLLLLIMTEPSDENRSSWRPEVQKLSVHALDMAAMRWEELDGIGEYSLFVDCAGRSAMACADAARCGVVANRVYFLVSSMHYEFGSEAPFHAFIPGSGREKFFDSWQALISDDRQWPPSQTWVYPSLFYRSRESLFVKLEHTPHVAARIDCSIL</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7S3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412750.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0412750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34361:SF11">
        <property type="entry name" value="DUF3741 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34361">
        <property type="entry name" value="OS08G0157800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1134" mass="123575" checksum="C38191FEBD778E30" modified="2022-05-25" version="1">MRDAHTTFATSSSRIHPSIHPRPGVDAMSYSGDRRPSPQPQPQPHTHAHLSYLSPSAAPFTVGHPRGAATPDPVPNAPANPSPYPDLPTAPSLYDSWVEPPASYMDLEAGAAAGYRGFANSDGFLVSENTHTGLYTGSHFGNTMQPHPFTTASSEWMEEKYPGIYQRTSKALPSDFGSSVIHQPSVSPNMFACLDKKRSTPQPVNQHSSYSAYDNYTTHLPSCSTYPLDYNLSMPPVSVSPEVCATTKSLSPTTDGHILENTFSSPYMNPCRLNLDYFDTMQNEQKDLFGHQTAYKHCGDWSNSDSGTRIMGSYPLSRRGVGENYPLGESSETGRPVQPGTYPLNGHGVGENYLSGDTSGSGRPVQPSSEVKSGFNSLQASSSNVSPSEHAFSQPRDLFIEPLEVNNPVVDSPCWKGTPTAQQSSFGVVKNDEAPFSANGSGDLHELHQSKKLSEFTTSNSVLFPKRHDTSNPANDSCLPYFVNYLSSSSLPSECKKSEGHSDAQPSIAGDFNCMARSSHPSYVSVDQGTRRDKHVTCKIGENSGNGVTPGQQGGVFLGQRTFEPIVLARDFGSHVVGMNEDSGKKVSSNFAAAPITKVRSLTKESLKGNTCVHKTVATLESLYSEMPMNIGLEHLTHCSPGVEESVKVSSDKVAYRSKTQEDLIKSIYNFSVVLLSTCDGGYELGESEHALVQSTIHNLSSLSSKISKAALKNDDANGKCHQSKSETIKCHGKNHQPENFDGLDWENIGADFKTFILEDLTKLPEENVIGDTKDAQMLIYKNLWIEAEASMCKLKYELQLARMKLVTKHYNQQTAAAPANSLGEAKAYMPKHKNSSCFGGFADSSKQQNHVKERNIGNAALLPQGGDKVDADVFARLKVLKLRDESINCYREVNAEVRKGTSNYNRTDVFDDTVFDNAVDDRITSLVEDIIKERSEASSSEGGESDGATIAALNGFTSCNNNTRSLDEGTNIEQAESSESKIHGAFMAKLKDLICCSDDISSSNEGNAHQLQTASKNEFGQLEDVVMARLQVLKRREDNNSSAVNEGQEVCHSDDWAGHFETKRLGRGAHDELIQKTDLPDNGGRRAQSDEADSKTASQYVSALLEEPCVVLKSPVLSRHQRNLQARTRMTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7Z4</accession>
    <name>A0A8I7B7Z4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin subunit beta-1/Transportin-1-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7Z4"/>
    <dbReference type="SMR" id="A0A8I7B7Z4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0158270.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0191020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0191020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0191020"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="7">
        <location>
            <begin position="229"/>
            <end position="544"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0191020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="610" mass="68602" checksum="FB354D5A56F35180" modified="2022-05-25" version="1">MELTFRALGGDVEPLKVQCIKLWITICEKEIDREEEEEEEEEEEEEEEEEEGEDLSASCFIGPLLRLVPLLLQTYIKEEGVVLKQEDIWKQRDEDEGDEDDGDEDVKQKGVNLEDMWEPEEDETLQVISMTCLGLAARIIKVEALHLVMEFIEELIIGPHMIAASFPLRFVLEGPSVKQLAPLVDLLLDLMIHSVEGVRGRAAWTLGRLFEIVGAHRIMRNKVWLLNRIMQLLIERSNDAPKVSMEVHRALYVLVRGYGEDAKSRSKPNSCELSPYVKPLIGALMYASDLSRETPSRFLASPYKALCEIVRVSDTQDLQVREALMGLISHIMRRFNVVLHGHNAISSPRRRNQLEVLLCGLVEVLIHKLGSTLQGSAKCLLLLLCPLLTRESTSARNGAALAIGALAHAIGADFGEHMPMVLLYFSVKRLSPLYLEVICDICHVLGKEGKEEEVVPCFDHIMEVLYQGMGELTLQPPILSSIGQIALSLGEKFEKYLPLVMEKLLVMENIARLDQGPHEDHSNKVREGICKAYHGIIGGISDAKSGFKVGMALLEFSEQERKRRDRDERTVTATDRRIMTALVDALSQLSPRVGVWSEAVILSVQQANIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8M0</accession>
    <name>A0A8I7B8M0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8M0"/>
    <dbReference type="SMR" id="A0A8I7B8M0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0459230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0459230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0459230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0459230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0459230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF7">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="115" mass="12735" checksum="A99156E271DCC139" modified="2022-05-25" version="1">MTRCPACPRTAPLKRLVTTTDKNGNLGREFVKCESKQEQGKKLKQCTHFEWLDEYIERIQLEGASRELDLPLEAEKFGSEAPGSGNSIGATVGDAAGTADLKKLNKQMKKLIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAD5</accession>
    <name>A0A8I7BAD5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAD5"/>
    <dbReference type="SMR" id="A0A8I7BAD5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0439990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0439990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0439990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0529010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF301">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="468" mass="52269" checksum="440B136BEBA4E431" modified="2022-05-25" version="1">MQELLLWEPITGVRQHVPVPMAYERTIFQPSAAVLCAADGCDHRDCLGGPFCLVFVFSVEDLDTEEEEYVTSACVYSSETGAWGELTSMHVEFMIDYTCYSRVLVARPLVYFMGTGGSILEYDLARHGLDVFEPPDTDLPLSKRFSLILMEDGRLGVSQSLDTDLKFWSREVATNGTDDARWVLSRVIELENLLPMRALARAEYSLLVLGFAEEANVIFINTVAGLFTIELHSERVRKVCDDHGFCNLVPVVGFYTPRSRLEVLRSEQHHALLPPLNIVEEVSGEEEDKKLQQVRKMFHEWCKDIEEGGFGNTTDLSSHALEFRVPLPLDRASKFYKCGRTFLCKAMKATNPSRNGSKSSSNEESIESTATATKYDTEGSEAFGSNAEQAPAEKVDSEEGKNLNGKDQEDGNLAGGGDDSDLDLAWKMLNVARAIVTKSPVMTMENFNIFHALAEVCKKRGNFSYVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAW1</accession>
    <name>A0A8I7BAW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123403106</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Secreted</location>
            <location evidence="2 14">Extracellular space</location>
            <location evidence="2 14">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the germin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953008.1">
        <property type="nucleotide sequence ID" value="XM_045097073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAW1"/>
    <dbReference type="SMR" id="A0A8I7BAW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543180"/>
    </dbReference>
    <dbReference type="GeneID" id="123403106"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0451730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0451730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0451730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0543180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0543180.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0543180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403106"/>
    <dbReference type="OMA" id="RKRFAPH"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000280">
        <property type="term" value="P:regulation of root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000025">
        <property type="entry name" value="germin-like protein subfamily 2 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035339974" description="Germin-like protein" evidence="14">
        <location>
            <begin position="22"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="15">
        <location>
            <begin position="64"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0543180.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="228" mass="24012" checksum="9A71BE708DD3D6FF" modified="2022-05-25" version="1">MAPHHLALLLLAVLLRAAATADPDAVQDFCVPDAGPGRPVELNLIRSYPCRSPGNVTAGDFAFSGVRAAGNFSADTGFAGVSVTPAQFPGLHTLGMSFARADLSAAGGVNPPHYHPRATETALVLAGRVYAGFVDTAGRLFAKVLVEGEVMVFPRGMVHFQLNVGDQPATVYGTFNSENPGIVRIPATVFGSGIRGAVLERAFGLTAEELRRIEKRFGVPKKADELED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB26</accession>
    <name>A0A8I7BB26_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB26"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0318940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0318940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0318940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0318940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0318940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0318940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="2">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0318940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="123" mass="13196" checksum="B9278CF04C13321A" modified="2022-05-25" version="1">MSNTTNGGNNGAATRGTPTTPPRPHWRTRDPTATVVYVVPSSQFRNVVQQLTGAASTNTASSEAQRYIVAPRVVHANEGHAGAGRRAEGGGGEDVDRDRTTTTTMKRMLEECMAWASDDHDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BCP0</accession>
    <name>A0A8I7BCP0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVA22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123449147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982184.1">
        <property type="nucleotide sequence ID" value="XM_045126249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BCP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0391590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0391590"/>
    </dbReference>
    <dbReference type="GeneID" id="123449147"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0325830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0325830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0325830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0391590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0391590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0391590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449147"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF98">
        <property type="entry name" value="HVA22-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="147"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0391590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="332" mass="36974" checksum="4590828FA2561BB7" modified="2022-05-25" version="1">MMGGFVSRILLLVFGYAYPAYECYKTVELNKPEIEQLIFWCQYWILVALMTVMERFGDLTISWLPFYSEAKLLFFIYLWYPRTKGTTYIYGTFFKPYISQHENEIDRNLLELRARASDVVVVYVQKVAAVGQNTFFDVLKYVASQSPSQKSKQQRSQEPQQPQQQQSQPQPQPQVQQQQPQKQAQPVMRRAASIAARQAAMAQQAQETKPVSPKIKRQASAKGGPAASVKPATAAASTAKPDENPKKSEAKPATAELVPAPATDAVVPRPVPKAQPAPDAEVSDDMAIDEADIAVEGAEELDPALEEETVMEETIRVTRAKLRRRAAAEGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFV3</accession>
    <name>A0A8I7BFV3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0752170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0752170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0752170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0752170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0752170.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0752170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="298"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0752170.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1477" mass="166180" checksum="6447927CBC07E60F" modified="2022-05-25" version="1">MDALLGAAQWVVGKALAPIADGVLEAWEASNNLGLNIETLKMELLLVKATLELASGKQIHGQAMELLLGKLRDSAHCAEDLLDELDYFRIHDQLQGTYDAADQHGKGGVHDIALNACHVAKAVGKLSFISSFCCSAPSPAGDSREVDGAIQRARCCACPRAMQRTTGNSSSAPNANREVSSCMPKLGKLLPCLSSPQVRQDRSGQPTLCGAPQGETPMPQFNRAHVSERMRHIVEQLQPVRKEVTQILQSCNPMTVPDIAQSRPITTSQSIEPNLYGRDRIMHTIIHDMTKGKYLSNDLTVLPIVGPGGIGKTTLIQHIYRNQQVQNHFQVVIWVCVSLSFNLKKLLEEIKTYIPRVEGEKDGRVEELIEQRLKSKRFLLVLDDIWEFSDGDDWKRLLLPLKTSQEKGSIILVTTRFPKITKMVGTTRHIELQGLESEEFRMLFLAFVFGNEPVRSDQCFLDEIGNKIMEKLRGSPLAAKTVGALLSKDLSLQHWRRVLESKEWETQTDSNGIMPALKLSYDHLSFQQQQCFFFSALFPEDHKYSATQLINLWIGLGILQPGARNQTLEIIGSNNLNDLVACGFFREEEEDGYPRYVMHDLLHDLALQVASHECLVVHHSSVRLEEIHPSIRHLSIIIHDADKISLENLKSELRKLKTSLKVKQLHTLMLFGEMDESFVSILGDLLGEAKSLRVLRLDDMPFSVESILHNFSTLLHLRYLYLGTECEREMNVLLTVSRFYHLRILALGSWCQNCHLPKELSNLVNMRHVYTPTVELHSDIFNVGKLRHLEELKVFRVNKESEGFELKQLEHLTELREVGIYNLENIHTKEEANKANLGVKNYLERLTLDWDSKRSNIEPDVEEIVLESLMPQRYLQDLCVRGHGGSSCPTWLGDKLAVEVLESLHLSGVSWEHLPPLGKMRGLVKVILENIATIEEFVIDQSSCRLVRLELVGLESFEKWVLSEGVDHMFPHLQVMIIRGCPKLLELPFQNCFFDPKPDQDQNICWFPKLQKLQIHKCPKLLLVARIPWTGTLQSVKIGDVRLLEKFRYSTSSYNGVDLEIVGKDELQSLDEVVAFNNLTGLEYLTLIKCPPMESKHLLLLTSLKKLDVQGSDDPVGPVGGGGDVDWQYPLEFLEVQKSCGGKQLTQLLTHLPRLSSLRIMDCEDITQLAAATTTEMELWKVDDDSGILLLPGHLSDTLQSLVIHGCLELVLVDSIRAGGGLQALQSLQTLSIFNSPKLLSTTRSSFSSCLFPSSLHRLILESLNGMETLEPLSNLTSLTKLHVKFCGEDLRCKGLGSLLTTGEQLRELTIFGSPKFFTGWDHNPRRVQLQGGGGEELVSPQSSSKLQMISTDEAMGLLAEPICSFLSSSLTELYLYGSRQEMEYFTKEQEDALQLLASLQRLHIYWYSKLQHLPARLHKLTNLKKLLVGYCPAVRSLPKDGLPKLLQELDVRDCGNEELKNECRGLVGTIPRIKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGS9</accession>
    <name>A0A8I7BGS9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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            <begin position="21"/>
            <end position="277"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
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        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
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    <dbReference type="SMR" id="A0A8I7BK48"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747540.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0620120.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0747540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0747540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0747540"/>
    </dbReference>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020:SF35">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020">
        <property type="entry name" value="OSJNBB0059K02.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0747540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33257" checksum="6BCFBC1CAF2BA487" modified="2022-05-25" version="1">MFVFGDDFVDNGNVPDITGEKTSRQWSYPYGSYRNSSWSGVPVPTGRFSNYRMQSDFIARMLGLHPTSKHQINLVTPFKRLVNDGVIETRQLHHSVALIAISGNDYMSGSDANNGFYSSFDDLDTYMGNVVTEILDNVAQLQTLGVRKVLVNNMHPIGCTPLQTSSNNYTTCDLLGNYGASVHNKYLKQMIGERDNVHILDLYTAFTDIVNHAPGEGSDRSKDFKRKLTPCCESSYKGGYCGKLSSSGKHLYDLCQNPDKRFYWDETHPTHAGWEAVMEALEQPLMEFLDQDYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F2CQT6</accession>
    <name>F2CQT6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123401209</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of a tri-snRNP complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNUT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK353988">
        <property type="protein sequence ID" value="BAJ85207.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950879.1">
        <property type="nucleotide sequence ID" value="XM_045094944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CQT6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623090"/>
    </dbReference>
    <dbReference type="GeneID" id="123401209"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0517040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0517040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0517040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0623090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401209"/>
    <dbReference type="OrthoDB" id="21368at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013957">
        <property type="entry name" value="SNRNP27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31077">
        <property type="entry name" value="U4/U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31077:SF1">
        <property type="entry name" value="U4_U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08648">
        <property type="entry name" value="SNRNP27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U4/U6.U5 small nuclear ribonucleoprotein 27kDa protein" evidence="9">
        <location>
            <begin position="151"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ85207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="209" mass="24611" checksum="AC891880240B98DF" modified="2011-05-31" version="1">MSDRRRDKERPRERGGYDQPRERDHDGHRDAERHRDSHRDRDRDRDRDVDRHRDRDRGQDKERDRDRDYRRARKRSRSRSPSPSADRDRSRRRRSHSHSHRSRSPDAGRHKRRREGSPVADPKEDRKPDLPSAPKAAEEAAPVGDGDVDAEEMEMMKMMGIPVGFDSTKGKYVPGADVSGVRAVTKRQPRQYMNRRGGFNRPLPPEVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2CVL7</accession>
    <name>F2CVL7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123445368</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK355670">
        <property type="protein sequence ID" value="BAJ86888.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978276.1">
        <property type="nucleotide sequence ID" value="XM_045122341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CVL7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314240"/>
    </dbReference>
    <dbReference type="GeneID" id="123445368"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0314240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314240"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPSA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CVL7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015081">
        <property type="term" value="F:sodium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF83">
        <property type="entry name" value="K+ POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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        </location>
    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="12">
        <location>
            <begin position="26"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="13">
        <location>
            <begin position="529"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="679"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
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        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ86888.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="785" mass="87663" checksum="84DBF8A25AA9F39C" modified="2011-05-31" version="1">MDAEAGAVAAAQELPWRQHYRNLLLLAYQSFGVVYGDLSTSPLYVYKSTFSGRLGQYQDEQTVYGVLSLIFWTFTLVPLLKYVIIVLSADDNGEGGPFALYSLLCRHAKLSLLPNQQAADEELSTYYRDGFAAQHGSSPWLRRFLEKHKTVKTGLLVVVLCAASMVIGDGVLTPAISVLSSMSGLQVRATGLQERSVVLLSCIVLVGLFSLQHRGTHKVAFMFAPIVIIWLLCIGGIGLYNIVHWNPKIYQAISPYYIVKFFRTTGTDGWIALGGILLSMTGSEAMFADLGHFTSASVRLAFITIIYPCLILQYMGQAAFLSKNMLHMRTSFYDSIPGPVFWPVFVVATLAAVVGSQAVISATFSIVKQCHALGCFPRVKIVHTSRWIYGQIYIPEINWILMVLCVAVTVAFGDTTLIGNAYGIACMTVMLITTFFMAFIIIFVWQKNIIFALLFLLFFGSIETVYLSSSLMKVHQGGWVPLVLAFIFMSVMFIWHYGTKRKYQFDLQNKVSMRSILSLGPNLGIVRVPGIGLIYTELVTGVPAIFTHFVTNLPAFHEVLVFLCVKSVPVPYVQPDERYLVGRIGPRAYRMYRCIVRYGYKDVQRDDENFENMLVMSIARFIMMEAEDVSSSASYDIANEGRMAVIRTTDDAGTPLGMRDLGGLAESISTTRSSKSESLRSLQSSYEQESPSANRRRRVRFELPNEDAMDQQVKDELLALVEAKHAGVAYIMGHSYIKARRSSNFLKKFAVDVGYSFLRKNCRGPSVSLHIPHISLIEVGMIYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2D020</accession>
    <name>F2D020_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123412797</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357227">
        <property type="protein sequence ID" value="BAJ88441.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045105740.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665270"/>
    </dbReference>
    <dbReference type="GeneID" id="123412797"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0551930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0551930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0551930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0665270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665270.1"/>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="entry name" value="TLDc_dom"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="OS06G0221100 PROTEIN"/>
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        <location>
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            <end position="340"/>
        </location>
    </feature>
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            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ88441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="362" mass="39844" checksum="B587D4694831E9BB" modified="2011-05-31" version="1">MGYLPSLGGKAAHLVSDLATVILNPVSGHERERSSHHLPEAAEGQENMYDDEESEIPNGPDTSSFRAFLMSFMSASSSSDDSMEIIPEQDVDMEYPTLTPVGRGNKERKGLLSRGKHSIGKIINKAGRLGGFRQKPSHIIDGEIASQIESVSSGFRPKVSKESASNHKLPAMSEPSMLLSEMMRNVLYPSLPVLVQGKNWMLVYSTWRHGTSLSTLYRRSMLCAGDSLLIIGDKKGAVFGGLVEAPLRPIMQRKYQGTKNCFVFTNVAGRPVIYRPTGANNYFTFCSPEYLAMGGGGHFALYLGEDLLNGSSSTSETFNNPCLSLSQDFEVKHVELWGFVNASRYDEMLTVCRTEKPGIWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F2D078</accession>
    <name>F2D078_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123407582</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldose epimerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357285">
        <property type="protein sequence ID" value="BAJ88499.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956682.1">
        <property type="nucleotide sequence ID" value="XM_045100747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D078"/>
    <dbReference type="SMR" id="F2D078"/>
    <dbReference type="STRING" id="112509.F2D078"/>
    <dbReference type="PaxDb" id="4513-MLOC_69935.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0663810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0663810"/>
    </dbReference>
    <dbReference type="GeneID" id="123407582"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0550680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0550680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0550680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0663810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0663810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0663810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407582"/>
    <dbReference type="eggNOG" id="KOG1604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031753_4_2_1"/>
    <dbReference type="OrthoDB" id="274691at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D078">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000012">
        <property type="entry name" value="Aldose 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF8">
        <property type="entry name" value="GALACTOSE MUTAROTASE-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ88499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="438" mass="47534" checksum="284E0AD196F00F0F" modified="2011-05-31" version="1">MEQLLGAQQHHSSLSPRTPTRPHLQHALSNRFRDHHHSHSHSQPHAGLKILRITPPFFLLLLAAVYLLASVTILSAPAPSLRASKKPDRLVLPVAAPVPPSRPPSPELLDLDNGRIRARISNVGAAVTSLLVPDKNGALADVVLGFDSLDPYLNGTSPYFGCIVGRVANRIKDGKFTLNGVQYSLGINNPPNTLHGGFKGFDKVIWEVIEYNKGKTPSITLKYHSKDGEEGYPGDVSVTARYSLPSSMELKLEMEAAPLNKATPISLAQHTYWNLAGHDSGDVLAHSIQIRGSQITPVDQISIPTGEFMQVSGTPFDFLTEAEIGERIGQVPGGYDHNYVLDSSREAKSSLRHVAKVTDPSSFRVLNIWSDAPGVQFYTGNFLGGIVGKGGAVYGKHAGLCLETQGFPNAVNQPNFPSVIVHPGEKYTHTMLFEFSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2D148</accession>
    <name>F2D148_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123448728</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the YOS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK357605">
        <property type="protein sequence ID" value="BAJ88819.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK376774">
        <property type="protein sequence ID" value="BAK07968.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981643.1">
        <property type="nucleotide sequence ID" value="XM_045125708.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981644.1">
        <property type="nucleotide sequence ID" value="XM_045125709.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981645.1">
        <property type="nucleotide sequence ID" value="XM_045125710.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981646.1">
        <property type="nucleotide sequence ID" value="XM_045125711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D148"/>
    <dbReference type="STRING" id="112509.F2D148"/>
    <dbReference type="PaxDb" id="4513-MLOC_60421.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379250"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379250.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379250.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379250"/>
    </dbReference>
    <dbReference type="GeneID" id="123448728"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0315490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0315490.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0315490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0379250.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0379250.2.CDS2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0379250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448728"/>
    <dbReference type="eggNOG" id="KOG4779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152125_1_1_1"/>
    <dbReference type="OMA" id="GANMIWN"/>
    <dbReference type="OrthoDB" id="776275at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013880">
        <property type="entry name" value="Yos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858:SF0">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08571">
        <property type="entry name" value="Yos1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090617" evidence="8">
        <location>
            <begin position="19"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ88819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0379250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="8535" checksum="48746C043F20DEB9" modified="2011-05-31" version="1">MGLWMLLEGFLLLANSLAILNEDRFLGPRGWSMSEVSGNGQTKSLKGQIVGLIYATQFLRMPLIALNVLIIVVKMVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2D535</accession>
    <name>F2D535_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123408468</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK358995">
        <property type="protein sequence ID" value="BAJ90206.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK361459">
        <property type="protein sequence ID" value="BAJ92664.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957507.1">
        <property type="nucleotide sequence ID" value="XM_045101572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D535"/>
    <dbReference type="SMR" id="F2D535"/>
    <dbReference type="STRING" id="112509.F2D535"/>
    <dbReference type="PaxDb" id="4513-MLOC_80913.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644320"/>
    </dbReference>
    <dbReference type="GeneID" id="123408468"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0535260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0535260.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0535260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0644320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0644320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0644320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408468"/>
    <dbReference type="eggNOG" id="KOG1270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WNRIYSE"/>
    <dbReference type="OrthoDB" id="66144at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D535">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046406">
        <property type="term" value="F:magnesium protoporphyrin IX methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010251">
        <property type="entry name" value="Mg_prot_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010940">
        <property type="entry name" value="Mg_prot_MeTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02021">
        <property type="entry name" value="BchM-ChlM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07109">
        <property type="entry name" value="Mg-por_mtran_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51556">
        <property type="entry name" value="SAM_MT_MG_PIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Magnesium-protoporphyrin IX methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="222"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ90206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ92664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0644320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="34740" checksum="6BBE8FECC68FA8D7" modified="2011-05-31" version="1">MARAAVSTAPLSRVHSPTPSVSFHPLSQLRTHQQRRPLTVASALPEVADLPGLSLPAAAAAAAALAAAVSLSDPERRRKAQAEAVGGGDKEAVRAYFNSTGFERWRRIYDPATDDVNRVQLDIREGHAQTVAATLAMLRDAPDVPLSGATVCDAGCGTGSLSIPLASSGASVLASDISAAMVSEAQRQAQAAAASSSPEFRMPRFEVRDLESLEGRYDIVVCLDVLIHYPREEAQKMIRHLASLAEKRLVISFAPKTLYFDVLKRVGELFPGPSKATRAYLHSEKDIEEALRAAGWRVKNKGFISTQFYFAKLYEAVPVAGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F2DCB4</accession>
    <name>F2DCB4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123440451</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK361531">
        <property type="protein sequence ID" value="BAJ92735.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK361613">
        <property type="protein sequence ID" value="BAJ92817.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362279">
        <property type="protein sequence ID" value="BAJ93483.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044972949.1">
        <property type="nucleotide sequence ID" value="XM_045117014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DCB4"/>
    <dbReference type="SMR" id="F2DCB4"/>
    <dbReference type="STRING" id="112509.F2DCB4"/>
    <dbReference type="PaxDb" id="4513-MLOC_69570.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298680"/>
    </dbReference>
    <dbReference type="GeneID" id="123440451"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0248920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0248920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0248920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0298680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0298680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0298680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440451"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_23_0_1"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DCB4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000157">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ92735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ93483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0298680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="270" mass="29318" checksum="0BA47A4DA7B65B47" modified="2011-05-31" version="1">MGRSPCCEKAHTNKGAWTKEEDQRLIGYIKAHGEGCWRSLPKAAGLLRCGKSCRLRWMNYLRPDLKRGNFTDDDDELIIKLHALLGNKWSLIAGQLPGRTDNEIKNYWNTHIKRKLLNRGMDPHTHRPISAAAASGLTASTTAPVFPSSPAPASRLASAPFASYPGSTVSFARPSPSDDGHSSSGGSSDAPRCPDLNLDLDLDLSMSLPCSPPKAAPPARSTLTSHQQQGICLCYHLGVRNGEACTCKTASPAGPRVFRFLRPLEEGQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2DGM3</accession>
    <name>F2DGM3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398656</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK363040">
        <property type="protein sequence ID" value="BAJ94244.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949042.1">
        <property type="nucleotide sequence ID" value="XM_045093107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DGM3"/>
    <dbReference type="PaxDb" id="4513-MLOC_16892.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515880"/>
    </dbReference>
    <dbReference type="GeneID" id="123398656"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0428600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0428600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0428600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0515880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0515880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0515880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398656"/>
    <dbReference type="eggNOG" id="ENOG502QT0N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="620353at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DGM3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ94244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0515880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="297" mass="32831" checksum="A15E1CF5AECE20F0" modified="2011-05-31" version="1">MHHGQQLPYHLSNTVIGYLNLLTLLASIPIIGAGLWLAHAASAAPGPTCQSALQAPLLAVGFVAFLVSLPGFVGARYHVSWALWLYLAAVLLLVLFLLGATVFGLAVTAGGGGRMVPGRPYREYRMRDYSPWLRSHVAADRYWRPALACVASSRACPKVAGWTPDDYMRRDLTPVQSGCCKPPTSCVYGDGAGSGPAVVAVQDEDCFRWQNDPAVLCYGCESCRAGVMEQLRRHWHNVTVVNAVLLLLLIAVCSCGCCAFRNARRAEYAYGGRMSKIHPRWDYFWSRWWRGHREQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F2DIB7</accession>
    <name>F2DIB7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123413229</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK363635">
        <property type="protein sequence ID" value="BAJ94838.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962107.1">
        <property type="nucleotide sequence ID" value="XM_045106172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DIB7"/>
    <dbReference type="SMR" id="F2DIB7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727920"/>
    </dbReference>
    <dbReference type="GeneID" id="123413229"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0603780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0603780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0603780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0727920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0727920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0727920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413229"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DIB7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090710" evidence="11">
        <location>
            <begin position="21"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="14">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="154"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="421"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="15">
        <location>
            <begin position="721"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ94838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="819" mass="87358" checksum="5BF0852383B2D4F6" modified="2011-05-31" version="1">MKGLGLACLLLALVPRVVLGTHDVYIVTMEGDPVVSYTGGVEGFPRTAADLDEEMEVTSEAVTSYSLHLRRHHEKLLDSLFVAGTYEKLYSYHHLINGFAVHMSSLQADFLRKAPGVKYVERDTKIQKLTTHTPQFLGLTTAVWPTGGGFDRAGEDVVIGFVDSGIYPEHPSFSTHRTDPYGPVPRYKGKCEIDPVTQRSFCNGKIVGAQHFAKAAIAAGAFNPDVEFASPLDGDGHGSHIAAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKVLYRLFGGYVSDVVAAIDQAVQDGVDILNLSVGPNSPPTATRTTFLNPFDAALLSAVKAGVFVAQAAGNGGPFPKTLVSFSPWITTVAAGVDDRRYKNHLILGNGKRIAGLGVSPATHGNKSFGLISATDALLGSSSTKYSALDCQRPELLNKRKVQGKILLCGYSFNYISGTASIKKVSQTARSLGAAGFVVAVEDSYPGTKFDPVPVNIPGILITDVSKTKDLIDYYNSSTTRDWAGRATAFQATVGIADGLAPTLFNSAPQVALFSSRGPDVKDFSFQDADVLKPDILAPGNLIWSAWAPNGTDEANYAGEGFAMMSGTSMAAPHIAGIAALIKQKYPKWSPSAIKSALMTTANTIDKGSHPLRAQQYSTSEMLTLTRATPFDYGSGAVNPKAALDAGLVLDATHQDYITFLCSIPDVDPSEVSNITGSRCSPSQKRQGPYDLNIPSITVSQLKGTQTVKRTVTNVADEAETYTIMTRMSPEIALDVSPPALTVLPGSSREITVTLTTRTVTGTYSFGEITMKGDRRHLVRIPVVAMGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2DNF8</accession>
    <name>F2DNF8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123451370</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK365426">
        <property type="protein sequence ID" value="BAJ96629.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984183.1">
        <property type="nucleotide sequence ID" value="XM_045128248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DNF8"/>
    <dbReference type="SMR" id="F2DNF8"/>
    <dbReference type="PaxDb" id="4513-MLOC_20244.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090990"/>
    </dbReference>
    <dbReference type="GeneID" id="123451370"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0074900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0074900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0074900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451370"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DNF8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF67">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="177"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ96629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="609" mass="65493" checksum="CCA728B24C146D62" modified="2011-05-31" version="1">MGVFGSSAKVYRPAPEVDLGPGSGELYISPNVKAPRVAGLLVKIFVWVLEMPVVGWVLLYILKKDNLINKLVSEAEIPEPPLFTSTHRWEDTPEQNVSLTKPGLSPAERVREAVDCLPARLESTLAADAPSSILKRWTIMDFSRAYSSGETTPVQVAKRFLAAVEESSGPTMNMAFFISCNPEDVLKQAEESTLRYQTGTPLSVMDGVLVAVKDEIDCLPYPTTGGTRWLGKARACEADAACVAQLRACGAILAGKTNMHELGAGTSGINPHHGSARNPYNVGKVAGGSSSGSAAVVCAGLCPVALGVDGGGSVRMPAALCGVVGFKPTAGRLSNAGVLPLNWTVGMAGILAGTVEDAVVAYSAIVDQSQPSYLRPELNLPMLTSTPSISNVRLAKYGKWFNDSSEDIRSCCDKALQTLRTQYGWETLDVTVPEIEEMRLAHYVTIGSECTASLAKYLDKLKRSEIGWDVRVALAVYGSFSSRAYLNSQRLRNRQMFFHKEIFKTADVIVSPMTGVTAYTLQDDALSSGELDYINGAALIRYSIAGNFLGLPAITVMVGQDKGGLPIGLQFIGRPWSEATLLHIAFAMQEACSKNHRKPAVFYDLLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQU4</accession>
    <name>F2DQU4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123452790</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK366262">
        <property type="protein sequence ID" value="BAJ97465.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985438.1">
        <property type="nucleotide sequence ID" value="XM_045129503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DQU4"/>
    <dbReference type="SMR" id="F2DQU4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446340"/>
    </dbReference>
    <dbReference type="GeneID" id="123452790"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0369630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0369630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0369630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0446340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0446340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0446340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452790"/>
    <dbReference type="OrthoDB" id="113620at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="7">
        <location>
            <begin position="7"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ97465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0446340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="491" mass="52664" checksum="B1157810E1C7A879" modified="2011-05-31" version="1">MAEARSRVAFVLVDGIGDVTIPSLGGRTPLEAAAAPRLDAVAAAGVVGLMDPVEPGLACGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMAPGDIAFKSNFATLDESTGVIVSRRADRHFEEEGPILCAALDGMKLPSFPEYEVRVRYATEHRCGVVVKGPRLSGNISGTDPLKDNRLHLKAKPLDDSEEAKNTAAVVNELSKEITRILVSQPINAKRADEGKNIANVVLLRGCGIRIEVPPFETKHGLTPCMVAPTKIIAGLGLSLGIDILEAPGATGDYRTLLTSKAKAIAKALSAPLETPPCVFVPGEDEYKGGRENGYDFGFLHIKAIDDAGHDKAVKLKVRGLEAVDQAIGQLARLLWEAEKSGHYQYFLCVTGDHSTPVEYGDHSFEPVPFAICRLRDFVGAIGEDNVMNTPLDDFPLPSVKSGEDLTDDSEPAERKPEERKAFSGDKVCEYTEIAAARGCLGRFPGSEMMGIIKKFMKAKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXB7</accession>
    <name>F2DXB7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123395138</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK368535">
        <property type="protein sequence ID" value="BAJ99738.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK370185">
        <property type="protein sequence ID" value="BAK01386.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044946006.1">
        <property type="nucleotide sequence ID" value="XM_045090071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXB7"/>
    <dbReference type="SMR" id="F2DXB7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534640"/>
    </dbReference>
    <dbReference type="GeneID" id="123395138"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0444790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0444790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0444790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0534640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395138"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03707">
        <property type="entry name" value="EFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF22">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="64"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ99738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="452" mass="48293" checksum="49EBA2FD6D86E940" modified="2011-05-31" version="1">MAAAAVLRGGSRRVLAYPALRAAVISGPAALPDVAAAAGPQPPPPSPLAAGLWARSMATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLSEAGSAKAVAFDEIDKAPEEKARGITISTAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAPDGPMPQTKEHILLARQVGVPSLVCFLNKVDAVEDEELLELVEMELRELLSFYKFPGDDIPIIRGSALSALNGTNEEIGKNAILKLMDAVDSYIPDPVRVLDKSFLMPIEGIFSIQGRGTVVTGRIEQGVIKTGEDVEVIGLTESGPVKTTVTGVEMFKKMMDHGEAGDNVGLLLRGLKRGDVERGQVVCKPGTVKTYKKFEAEIYVLTKDEGGRHTAFFSNYSPQFYFRTADICGKIELPPDVKMVMPGDNVTAIFELMLPVPLEPGLRFALREGGRTVGAGVVAKVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0L1</accession>
    <name>F2E0L1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123429689</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369682">
        <property type="protein sequence ID" value="BAK00883.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969637.1">
        <property type="nucleotide sequence ID" value="XM_045113702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0L1"/>
    <dbReference type="SMR" id="F2E0L1"/>
    <dbReference type="STRING" id="112509.F2E0L1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0124520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0124520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0124520"/>
    </dbReference>
    <dbReference type="GeneID" id="123429689"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0102640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0102640.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0102640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0124520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0124520.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0124520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429689"/>
    <dbReference type="OMA" id="WIMEFDS"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK00883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0124520.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="89" mass="10260" checksum="BB9087E934E9D325" modified="2011-05-31" version="1">MPIRGVPCAWTVEEFKSWLKQFDVDRDGKISKAELRQAIRRRGCWFATARAGRAVRRADRDHNGYVDDAELENLVAFAREHLGMNISAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0X4</accession>
    <name>F2E0X4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="AK369795">
        <property type="protein sequence ID" value="BAK00996.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0X4"/>
    <dbReference type="SMR" id="F2E0X4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0565380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0565380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0565380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0681050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0681050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0681050"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045506_2_0_1"/>
    <dbReference type="OMA" id="CCNEAIS"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E0X4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF96">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090826" description="chitinase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 19 catalytic" evidence="10">
        <location>
            <begin position="56"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="254"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAK00996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0681050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="304" mass="33424" checksum="8F219CB0FF6C0145" modified="2011-05-31" version="1">MGMVRAASFLLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLAHAAGFWNYQAFITAASLFEPRGFGTTGGREMSMKEVAAFLGHVGAKTSCGYSLATGGSLAWGLCYNHEMSPSQSYCDDSNELYRCAEGVGYYGRGALPVYWNYNYGIVGKGIKQDLLNHPELLEQNATLAFEAAIWRWMTPMKRKQPSAHDAFVGNWKPTKKDTLSKRYPGFGVTMNILYGDAICGKGTTESMNAIISHYQHYLDLMGVGVQHSGDNLDCADQVPFNPSSKSPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F2E343</accession>
    <name>F2E343_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123413277</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the regulation of gamete interactions during the double fertilization and to prevent multiple-pollen tube attraction; mediates the redistribution of the gamete fusogen HAP2/GCS1 to the cell surface after secretion upon sperm arrival.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the plant egg cell-secreted peptide family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370566">
        <property type="protein sequence ID" value="BAK01765.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962149.1">
        <property type="nucleotide sequence ID" value="XM_045106214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E343"/>
    <dbReference type="STRING" id="112509.F2E343"/>
    <dbReference type="PaxDb" id="4513-MLOC_60623.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0732390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0732390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0732390"/>
    </dbReference>
    <dbReference type="GeneID" id="123413277"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0607790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0607790.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0607790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0732390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0732390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0732390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413277"/>
    <dbReference type="eggNOG" id="ENOG502S3PF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128969_1_0_1"/>
    <dbReference type="OMA" id="EAQQCWE"/>
    <dbReference type="OrthoDB" id="776947at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E343">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009567">
        <property type="term" value="P:double fertilization forming a zygote and endosperm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080155">
        <property type="term" value="P:regulation of double fertilization forming a zygote and endosperm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000008">
        <property type="term" value="P:regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044711">
        <property type="entry name" value="EC11-15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008502">
        <property type="entry name" value="Prolamin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35293">
        <property type="entry name" value="EGG CELL-SECRETED PROTEIN 1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35293:SF4">
        <property type="entry name" value="PROLAMIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05617">
        <property type="entry name" value="Prolamin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0278">Fertilization</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090840" evidence="10">
        <location>
            <begin position="25"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Prolamin-like" evidence="11">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAK01765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0732390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="17558" checksum="0285762F139B7B52" modified="2011-05-31" version="1">MASSGPLLPTLLVLLAAAAATASAAGARPASTTTATTFVRAADLADRLEGAVSQQCWETLLHIKSCTGEIILFFLNGEAYLGPGCCRAIRAIEQRCWAADLMLSVIGFTPEEGDMLKGYCDAGDDDNNNGPRHSFGGSSPAPPPRRALGADGVATGAGTVAAAAGRKGLGAPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2E692</accession>
    <name>F2E692_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123443118</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK371666">
        <property type="protein sequence ID" value="BAK02864.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044975319.1">
        <property type="nucleotide sequence ID" value="XM_045119384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E692"/>
    <dbReference type="SMR" id="F2E692"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244240"/>
    </dbReference>
    <dbReference type="GeneID" id="123443118"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0202690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0202690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0202690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0244240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0244240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0244240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443118"/>
    <dbReference type="OrthoDB" id="2498029at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000131">
        <property type="entry name" value="Monoglyceride lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="3">
        <location>
            <begin position="130"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK02864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0244240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="389" mass="43015" checksum="BBBE22E527E56516" modified="2011-05-31" version="1">MEMGGDEMMMTSGATGRIVPVFRSVLSRRALLRLAVALHSLFLWLLLLVGRRRRPDAASPAEPGRPGKARSRRRAAEEEDVRRRRALAEEVPMAEDDSDADGRTCGTFVIAGARRNALFCRVWAPAVGTETRAILLIVHGLNEHSGRYLHFAEQLTSCGFGVYAMDWIGHGGSDGLHGYVPSLDYVIKDMEVLLDKIMLENPDVPCFLLGHSTGGAVVLKASLYAHIRTRLEGIILTSPAVRVKPAHPIVGAVAPIFSLIAPKFQFKGANKRGIPVSRDPAALLAKYSDPLVYTGPIRVRTGHEILRISSYLLHNLKKVTVPFVVLHGTADRVTDPLASQELYREAASRHKDLRLYEGFLHDLLFEPERDEIAADIIRWMDRTLGLQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2EG84</accession>
    <name>F2EG84_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123440862</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK375161">
        <property type="protein sequence ID" value="BAK06356.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973341.1">
        <property type="nucleotide sequence ID" value="XM_045117406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EG84"/>
    <dbReference type="SMR" id="F2EG84"/>
    <dbReference type="STRING" id="112509.F2EG84"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0316100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0316100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0316100"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0316100.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0316100.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0316100"/>
    </dbReference>
    <dbReference type="GeneID" id="123440862"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0263910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0263910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0263910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0316100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0316100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0316100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0316100.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0316100.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0316100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440862"/>
    <dbReference type="OMA" id="AMASEQM"/>
    <dbReference type="OrthoDB" id="610733at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EG84">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK06356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0316100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="361" mass="40182" checksum="2A6B3AE1214F71B1" modified="2011-05-31" version="1">MASQPMVHMSHGQGETSYARNSSFQKAEQNRMKSLIEDVIADLCGSSSTIFPGKLVIADLGCSSGPNALALVSTAVNAIHSQCLQLQQPPPEVCVLLNDLPDNDFNTVVKSLVTLRKSKNPVVLTGVIPGSFYERLFTSDSLHLVCASNSLQWLSKAPEDLTRNHIPAFDIDEHVRRERLPMVREAYAHQFRKDFTLFLKLRAKELVSGGRMVISLVGTRSDVIASKFFLFPGIVAQILTVMVAEGMIDKAKFDSFYVPVHGPSSEEVREIIEVEGSFLIKEMRVHNPTTEMNTALSTPRKFVNNLRALFEPIIVQHFGEIMDEFVRTAELHWNLDLDGTLQEERARTSRAMLVVSLAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2EJI3</accession>
    <name>F2EJI3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123401672</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Flower</tissue>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376310">
        <property type="protein sequence ID" value="BAK07505.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951453.1">
        <property type="nucleotide sequence ID" value="XM_045095518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EJI3"/>
    <dbReference type="SMR" id="F2EJI3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0548630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0548630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0548630"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0765320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0765320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0765320"/>
    </dbReference>
    <dbReference type="GeneID" id="123401672"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0455890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0455890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0455890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0548630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0548630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0548630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0765320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0765320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0765320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401672"/>
    <dbReference type="KEGG" id="hvg:123418612"/>
    <dbReference type="OrthoDB" id="10253031at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="118"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK07505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0548630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0765320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="149" mass="15784" checksum="AF7BD92FAB4A4F47" modified="2011-05-31" version="1">MAGRKGGERKKAVARSVKAGLQFPVGRIGRYLKKGRYAQRVGSGAPVYLAAVLEYLAAELLELAGNAAKDNKKTRIIPRHLLLAVRNDQELGRLLAGVTIAHGGVIPNINSVLLPKKSPAAAEKEATKSPKKKAAATKSPKKKAAATKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKD0</accession>
    <name>F2EKD0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123401723</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Flower</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK376607">
        <property type="protein sequence ID" value="BAK07802.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951513.1">
        <property type="nucleotide sequence ID" value="XM_045095578.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951514.1">
        <property type="nucleotide sequence ID" value="XM_045095579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKD0"/>
    <dbReference type="SMR" id="F2EKD0"/>
    <dbReference type="PaxDb" id="4513-MLOC_62630.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0625260"/>
    </dbReference>
    <dbReference type="GeneID" id="123401723"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0518820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0518820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0518820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0625260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625260.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0625260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401723"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031019_1_0_1"/>
    <dbReference type="OMA" id="EECPCIS"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EKD0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000690">
        <property type="entry name" value="F-box/LRR-repeat protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF271">
        <property type="entry name" value="COI1 F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="360"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK07802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625260.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="483" mass="54076" checksum="E7F564BBE72F6606" modified="2011-05-31" version="1">MEDLPEPLLADIIKRITVSSDLNSLCLVSKRLCAAEAEHRGAIRVGCGIDPTTEAFTSLFSRFSNLWKVEISYFGWTPSHGDQVNNQAILLLSHHCPLLSDLTLSFCAHIDDAGLSYLPYCKKLRSLKLNFAPAITSTGIFRVAVSCCYLSVLHLVDCIAIESVEWLEYLGRYGSLRELVLKDCDGISQYDLLKFGPGWRKLQRFEFEINGTYWLSERPDPSYVVGYPYSYDICCDNLKDLRLAHIVTKPEIGLRFLLGKCKALETLYLEYVIGLNENEMIALFQRCCNLKTISLRLMPLRCEDHRFRTPLTDDSLKVLALSCPMLEVVELTFTFCEPTYPTEIGFTQKGIVTLIQSCPIRALVLNGASIFDDEGMKGLSCTQFLEKLDLVDCCSVSDAGMYLIAQAPCLNHLTLRKCSSVTNNGLAALAHSQKLESLTVIGCRWISQEGVQGAAKSVCYSSETESHDNLKGMNVYRKIRQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>M0UWF8</accession>
    <name>M0UWF8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">lipid-A-disaccharide synthase</fullName>
            <ecNumber evidence="1">2.4.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123444425</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a lipid A disaccharide + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="ChEBI" id="CHEBI:176338"/>
            <dbReference type="ChEBI" id="CHEBI:176343"/>
            <dbReference type="EC" id="2.4.1.182"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.1.182" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044977063.1">
        <property type="nucleotide sequence ID" value="XM_045121128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0UWF8"/>
    <dbReference type="SMR" id="M0UWF8"/>
    <dbReference type="FunCoup" id="M0UWF8">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0UWF8"/>
    <dbReference type="PaxDb" id="4513-MLOC_15004.15"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289240"/>
    </dbReference>
    <dbReference type="GeneID" id="123444425"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0240200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0240200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0240200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0289240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0289240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0289240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444425"/>
    <dbReference type="eggNOG" id="ENOG502QTT8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0UWF8"/>
    <dbReference type="OMA" id="YVILPFE"/>
    <dbReference type="OrthoDB" id="2419at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0UWF8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008915">
        <property type="term" value="F:lipid-A-disaccharide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003835">
        <property type="entry name" value="Glyco_trans_19"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00215">
        <property type="entry name" value="lpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372">
        <property type="entry name" value="LIPID-A-DISACCHARIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372:SF4">
        <property type="entry name" value="LIPID-A-DISACCHARIDE SYNTHASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02684">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0289240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="481" mass="53617" checksum="D811F8837BC1C989" modified="2013-04-03" version="1">MLSRWIPAAGRQRGLLGRLRSRAYSSGRVFDAAVRDGELRVFVVAGEVSGDSLASRLMASLRKLSPVPVRFAGVGGELMCKEGLQSLFPMEEIAIMGLWELLPHIYSVKRKIKDTLEAAILFRPHAVVTVDSKGFSFRLLQQLKCRYSQKVDSPIHVHYVAPSFWAWKGGESRLPKLHNFVDHMLCILPFEDEICRLNGLPATYVGHPLLDDAAGLNVAGTSLQHKNVDSELSPDMSMRQQSGEAFRLENGLSPDATILTMLPGSRMQEVARMLPIFFRTVQHLSHTLNDLSLVIPVAPHRDVRTYVENVVRSVPFPVILIPGGSLEKRYGAFNASKAALCTSGTAVMELMLAKLPCVVAYQAHFLTECFIHLRKKINFISLPNILLDSPIVPEILFRACTDKNLAAKLSEVIFNDEVRQLQVESAERMLQVLYEPIKQRGNLFAEELGDSSLSSDVHSPSTIAALTVLYTDRNRRIVHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>M0VJ21</accession>
    <name>M0VJ21_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401433</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951181.1">
        <property type="nucleotide sequence ID" value="XM_045095246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VJ21"/>
    <dbReference type="SMR" id="M0VJ21"/>
    <dbReference type="FunCoup" id="M0VJ21">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0VJ21"/>
    <dbReference type="PaxDb" id="4513-MLOC_30839.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592070.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592070"/>
    </dbReference>
    <dbReference type="GeneID" id="123401433"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0490710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0490710.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0490710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0592070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0592070.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401433"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0VJ21"/>
    <dbReference type="OMA" id="CEWAYLL"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF45">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592070.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="57201" checksum="068B8B95C030568E" modified="2013-04-03" version="1">MSQLSLWRRHGARRALQLLPPCRLSLSSVTHLNRDLNSIDLTMEEEALAAQIKGSLLKARNGDVQDLVQSLGVDCPGIQPTSNTVDSLLCKFGDDWKSAFSLFQWAQLSGNYKHTAYACSRMIDLLGKMRQIDRMWDLLSDMHCRGLVTVETAAKSIRRLAGARRWKDAVMLFDKLEDMGLERNTETMNVLLDVLCKEKKVEVARKVFLVLSPHIQPDAYTFNIFVHGWCSARRIDEAKWTIEEMKARGFPPSVITYTAVLEAYCKQHNFRMVDEVLDSMCSEGCHPNVITYTMIMTSLSKCYMFEEALSVSHRMKASGCKPDTLFYNSLINVLGKAGHLSEASQVFQLEMPMNGVPHSLATYNTMISIFCQKDRDEDAINVLKEMEAHSCKPDLQTYRPLLRLFLSRRGQYHSIRNLLNELINKQSLCLDVDTYSLLIHGLCRVGETDWAYQLFEEMVGSEIVPRYKTWELLLNEAQRKNMEGRAERIRNYMTCFGLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>M0YGA7</accession>
    <name>M0YGA7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123449876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044983168.1">
        <property type="nucleotide sequence ID" value="XM_045127233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YGA7"/>
    <dbReference type="SMR" id="M0YGA7"/>
    <dbReference type="STRING" id="112509.M0YGA7"/>
    <dbReference type="PaxDb" id="4513-MLOC_69029.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412420"/>
    </dbReference>
    <dbReference type="GeneID" id="123449876"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0343640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0343640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0343640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0412420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449876"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0YGA7"/>
    <dbReference type="OMA" id="DGQYWNK"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YGA7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033592">
        <property type="term" value="F:RNA strand annealing activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034057">
        <property type="term" value="F:RNA strand-exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097010">
        <property type="term" value="P:eukaryotic translation initiation factor 4F complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12271">
        <property type="entry name" value="RRM1_PHIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034361">
        <property type="entry name" value="PHIP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF108">
        <property type="entry name" value="OS03G0123200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="70"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="170"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="249" mass="27968" checksum="F145B773A7F921DF" modified="2013-04-03" version="1">MAAEDQIPRAQANPEKSKKRKKPKKDKWGQPIAAAADERAVEQVQEQETPEEPAVAAAEVGEGAESYECGKVVASGMPYTTTEEEIRELFDRFGPIRSLQLSRFPDSGNFRGLAFVTFESDEVALKSLELDGYKIGNRFMRVERCRITASSKRQKKTEFQSDPEKPDGCLSAYVGNLSWNISEKDLRDFFKSSRIASIRFAIDKRTGDSRGFCHVDFEDDESLEKAVGMNQSELRGRPVKIAYAIINRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>M0YLM6</accession>
    <name>M0YLM6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123449386</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982526.1">
        <property type="nucleotide sequence ID" value="XM_045126591.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982527.1">
        <property type="nucleotide sequence ID" value="XM_045126592.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982528.1">
        <property type="nucleotide sequence ID" value="XM_045126593.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982529.1">
        <property type="nucleotide sequence ID" value="XM_045126594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YLM6"/>
    <dbReference type="PaxDb" id="4513-MLOC_70558.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398400"/>
    </dbReference>
    <dbReference type="GeneID" id="123449386"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0331450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0331450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0331450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0398400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449386"/>
    <dbReference type="OrthoDB" id="624345at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YLM6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="606" mass="66640" checksum="163786DA334BD5C3" modified="2013-04-03" version="1">MKFMKLGSNPDTFQDDGGEVRIVESELVSDITVRLGNTKFYLHKFPLLSKCARFQKLIPTTGDENIDIHIHDIPGGSKAFEICAKFCYGMVVTLNAYNVVAARCAAEYLEMNETVDKGNLIYKIDVFLSSSIFRSWKDSIIVLGTTKAHLPWSEDLKLVSHCIDSIASKASVDTSKVEWSYTYNRKKLPTETDLDMQWNGVKKQPSVPKDWWVEDLTDLDIDSYKQVISAIKTMGMVPKDAVGEAIKAYTYKKLPSLSKVSMIHGDAKVRAMLVTITCLLPSEKGSVSCSFLLKLLRATNLLKCGEMCRKELVKRIGRQLDEASVSDLLIPTVDGETTVYDVDMILSIVEEYVKQDSKNAQKHNAEVNGHVQAPSASMITVAKVVDGYLTEVAKDPNTPVLKFINLAEAISGNSRPFHDGLYRAIDMYLKEHPSLSKSDKKKLCCLMDCKKLSPDACAHAVQNERLPLRTVVQVLYHEQTRASAAATVRADSICIGSYESSRSGATTNTEDEWDGVIAVEDLSLSSKTTKLEKCHSTEKSHGSSKSTANGKAKGGATPKKVLGKMLSSKALTGERSSSDSSDSAILRSQDHPKRTPSRSTKPSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GD92</accession>
    <name>A0A287GD92_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dihydrodipicolinate reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287GD92"/>
    <dbReference type="SMR" id="A0A287GD92"/>
    <dbReference type="STRING" id="112509.A0A287GD92"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0064320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078980"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287GD92"/>
    <dbReference type="OMA" id="FHIECWA"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000286">
        <property type="entry name" value="Dihydrodipicolinate reductase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000074">
        <property type="entry name" value="Dihydrodipicolinate reductase, C-terminus family protein, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836:SF6">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE-LIKE PROTEIN CRR1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydrodipicolinate reductase N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydrodipicolinate reductase C-terminal" evidence="9">
        <location>
            <begin position="123"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="27140" checksum="D19086528E0EC563" modified="2017-11-22" version="1">MGRVAIAAVSRARRMELAGAIDTQCIGMDVGELTGMDEALEIPVLNDLTMVLGSIAQTRATGVVVDFSEPSSVYDNVKQAAAFGLSSVVYVPKIEMDTITELSAFCDKASMGCLVAPTLSIGSVLLQQAAIQASFHYNNVEIVESRPNPSDLPSPDAIQIANNISDLGQIYNRQDMDSHNPARGQVLGEDGVRVHIMVLPGLASSTSVVLSGPGEVYTLKHDVTDVQSLMPGLILAIRKVIRLKNLIYGLEKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GXM1</accession>
    <name>A0A287GXM1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123429111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969112.1">
        <property type="nucleotide sequence ID" value="XM_045113177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287GXM1"/>
    <dbReference type="SMR" id="A0A287GXM1"/>
    <dbReference type="STRING" id="112509.A0A287GXM1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0098810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0098810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0098810"/>
    </dbReference>
    <dbReference type="GeneID" id="123429111"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0081400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0081400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0081400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0098810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0098810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0098810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429111"/>
    <dbReference type="InParanoid" id="A0A287GXM1"/>
    <dbReference type="OMA" id="STANRCC"/>
    <dbReference type="OrthoDB" id="605712at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF620">
        <property type="entry name" value="OS01G0223000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0098810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="42004" checksum="7E03CC7B81A8A9FB" modified="2017-11-22" version="1">MHKLVCAALLLLSWSKQAISSPSGAPRYRSIFSFGDSFADTGNNPAVFGWYSVFDPVTWPPYGTSFFGRPTGRNGDGRLIIDFIAENLGLPYVPPTLAHNGSFRRGANFAVGAATALDASFFHERDIPGGASKFPLNTSLGVQLEWFESMKPSLCRTARECKKFFSRSLFLVGEFGVNDYHFSFQRKTVQEVRSFVPDVITTISIAIERLITHGARSLVVPGVIPSGCSPPILTKFANAPPAAYNSETGCLTAHNELGLHHNTLLQAELDSLRAKHSNVRIIYADFFGPIMEMVESPHKFGFEDDVLMVCCGGPGRYGLNSTVPCGDAAATTCPDPSARLYWDGVHLTEAANRHVADVWLGEINSSTGASRKQGTKGPCRLGRHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287KDF1</accession>
    <name>A0A287KDF1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123442958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="STRING" id="112509.A0A287KDF1"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
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    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="entry name" value="WRKY transcription factor 57"/>
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    </dbReference>
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        <property type="entry name" value="WRKY domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF17">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
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    </dbReference>
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        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0238240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="264" mass="27917" checksum="61A2342215C8AAC5" modified="2017-11-22" version="1">MMFPSPGGAAMALAHGQAASRGATTAGGAATAITFSFQQPSDPPLNGGLSHHHGLLGYSPLVLDHPTTTTSSSSTIPAAPSLHHLHAPAGAIHPPRSSPPHPWSSEEADRERQRGKAAVTGMAVNSAPAGGSGGGGRSHGPAAAVAAGMGVGAVRMKKAAGGGAVKARRKVREPRFCFKTMSDVDVLDDGYKWRKYGQKVVKNTQHPRSYYRCTQDKCRVKKRVERLAEDPRMVITTYEGRHVHSPSRDDDDAARANAEMSFIW</sequence>
</entry>
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            <fullName evidence="7">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC123441176</name>
    </gene>
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        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045117680.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="112509.A0A287MT33"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0329100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0329100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0329100"/>
    </dbReference>
    <dbReference type="GeneID" id="123441176"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0274030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0274030.1"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="428"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="584" mass="63724" checksum="1B9EDA9015ABA5E0" modified="2017-11-22" version="1">MADTASTTSSSSGGEVAPLQMDKRLLNVATSGDSKSMQSLATQNPGILLGTTPTGNTCLHIASTLGHQGFCMDVVALDNSLLTVENMDGETPLLAAVASGRALVASVLLRCYQARQMSAAILGQDKEGCNVLHHAIHSGHRELALELIEAVPALSKHVNKHDESPMFIAAMRNFPHIFEKLLSIPDSSHAGGYGCNALHAAARDGDEERTKKIMRARPQMAIVANDGHNTPARLAVLFNKTKVVRILLQHDCSLGYEVNKQGRTLLGSAAFRGHVDIAREILKHCPDAPYCRTSDCRTWLHTSVLFGHEEFVEFIVETPQLSKLINMQDNTGKTALHYAVQKCNPRIVASLLSHKGIDATTMDKNGSPAGWELLGAVEKAKTLNWNEVAMLMSKACPEDAPVLHNLRVQAKREMTDASRKHVKSLTQTYTSNTSLVAILIATITFAAAFTLPGGYSNDAGSEGLPIMSKKFAFQAFLISDVLAMCSSFAVAFICIIARWEDYEFLIYYRSFTKKLMWFAYVATTTAFSSGLYTVLASRLQWLAIAICVLVAVLPIVTKLLGKWPVWSLKIRLGKSYRSDLLSMV</sequence>
</entry>
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    <accession>A0A8I6W5B7</accession>
    <name>A0A8I6W5B7_HORVV</name>
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        <recommendedName>
            <fullName evidence="21">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="19" type="primary">LOC123428477</name>
    </gene>
    <organism evidence="19 20">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VSR (BP-80) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968640.1">
        <property type="nucleotide sequence ID" value="XM_045112705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6W5B7"/>
    <dbReference type="SMR" id="A0A8I6W5B7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0034590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0034590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0034590"/>
    </dbReference>
    <dbReference type="GeneID" id="123428477"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0026950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0026950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0026950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0034590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0034590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0034590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000178">
        <property type="entry name" value="vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000001">
        <property type="entry name" value="Vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035279637" description="PA domain-containing protein" evidence="16">
        <location>
            <begin position="27"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="573"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="17">
        <location>
            <begin position="65"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="18">
        <location>
            <begin position="195"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0034590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="70726" checksum="8D1D742457235805" modified="2022-05-25" version="1">MWGLGFRRRLLLLLLLAFAAAVKLEARFVVEKNSLMVTSPTALRGRHDSAIGNFGIPQYGGSMAGAVVYPMGNSDACKAFDGGRKEHLFRTKPGALPSFLLIDRGNCLFAKKVWNAQDAGASAVLVVDDKDEPLITMDLLQEDDEAAKYIQNISIPSALIDKKFGEQLKKAVKDGEMVNVNLDWREAVPHPDNRVEYELWTNSNDECGPKCDMLMHFLKEFKGAAQLLEKGGYSQFTPHYITWYCPQAFVVSKQCKSQCINHGRYCAPDPEQDFSTGYDGKDVVVENLRQLCVFNVANVIKKPWIWWDYVTDFHIRCPMKDKKYNKKCAETVIKSLGLEVKKIDKCMGDPNDDSDHPLLKMEQDSQIGKGSRGDVTILPTLVVNNRQYRGQLGRKAVLKAICAGFEETTEPNVCLSDDMETNECLSDNGGCWQDKAANVTACRDTFRGRVCECPTFNGVQFKGDGYNNCEPAGPGKCLINHGGCWHETRNRKTFSACQESGERNCKCPAGFRGDGVKKCHDIDECKERKACQCPECSCRDTWGGYDCTCSGDLLYIKEHDTCISKTAVKAKEAWAAVCGILVALVIVAAGGYVVYKYRLRSYMDSEIRAIMAQYMPLDSQGEVPNDSHEGHPLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WC61</accession>
    <name>A0A8I6WC61_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6WC61"/>
    <dbReference type="SMR" id="A0A8I6WC61"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0137760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0137760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0137760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0137760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0137760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0137760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF9">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="7"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="383"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0137760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="440" mass="47967" checksum="C817398684FBE612" modified="2022-05-25" version="1">MALSSGILALSIIGVLVAGIVTVVATIAIYLCAKVAVQMYLHKEDKLGGHRDAWLGNGAAAGITPRAAQNEADDVEMGSMRFFIEGVQNEQPVHFISQQLRGFTQGFAHKVGSGGFGVVYMGRFPDGTSVAVKALVYEYMVNGSLDRHLFGTPAPDGRVIEFDKLHEIAVGTAKAVRYLHEECQHRIIHYDIKPENVLLGADMAPKVSDFGLAKLCDTEDTHLTITGAWGRPATRHRSCGCRCRRESQEWYPRWVWHRFEAGDMDVVLARAMAAGDLDDTEKAARMCKVALWCVQFRPEDGPSMGNVVRMLEGEEQIATPGNPFAHHAAYSAGATMTDVTTTESYGSSAFTRTGLAVLASSAPPPPPCRPRLLRRLHPAVLRVRRRPRAEGQAAGRRGRAAAAGLVKSPRRPHPRRLDLDRRRASSLLTLTTGGPRVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WIM9</accession>
    <name>A0A8I6WIM9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123429951</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044969860.1">
        <property type="nucleotide sequence ID" value="XM_045113925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WIM9"/>
    <dbReference type="SMR" id="A0A8I6WIM9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0039350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0039350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0039350"/>
    </dbReference>
    <dbReference type="GeneID" id="123429951"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0030460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0030460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0030460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0039350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0039350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0039350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000024">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 5, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0039350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="184" mass="20984" checksum="89FAAACD05FC9CD0" modified="2022-05-25" version="1">MSSPSKRREMDLMKLMMSDYKVEMVNDGMQEFFVEFRGPTESIYQGGVWKVRVELPDAYPYKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAALMMRDRPAYEQKVKEFCDKYAKPEDAGITPEDKSSDEEELSEDEDDSGDEDIVGKPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQB5</accession>
    <name>A0A8I6WQB5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC123425448</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044965087.1">
        <property type="nucleotide sequence ID" value="XM_045109152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQB5"/>
    <dbReference type="SMR" id="A0A8I6WQB5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128600"/>
    </dbReference>
    <dbReference type="GeneID" id="123425448"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0106100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0106100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0106100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425448"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002215">
        <property type="term" value="P:defense response to nematode"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009825">
        <property type="term" value="P:multidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009845">
        <property type="term" value="P:seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000388">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000691">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF22">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 25" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035326018" description="non-specific serine/threonine protein kinase" evidence="25">
        <location>
            <begin position="24"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="821"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="850"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1121" mass="119551" checksum="8A3B45F2635C8C40" modified="2022-05-25" version="1">MSPLLLQSLCILLLAGEVVLIGAQSAGGGGGGDKEVLVELKRFLVSNNKVNRGGYDGWQESDPSPCGWKGVTCDGGGRVSSLNLTRSTISGPVFGGFSRLPALTSLDLSDNSITGALPAADLNQCRGLLHLNLSHNLITGPLVLSGLTRLRVLDVSGNRLDGAVAVNFPAICADLTLLDLSTNNLTGSVTGLLDGCARLDKVDLSSNNFTGELWPGIARFREFSAAENNLTGSVPWSTFPDGCRLQSLDLSANQLVGGFPDSIANCTNLTYMSLWGNNFTGKIPAGIGKLAVLETLILGKNKFDRQIPPDLTNCGRLQFLDISSNMFGGDVQQIFGNFTSLKYLVLHHNEYTGGIVASGVLRLPLLARLDLSFNQFTGQLPPQVADMKSLKYLMLAENNFSGTIPPEYGRLAELQALDLSNNTLSGVIPATIGNLTSLLWLMLAGNQLSGQIPPEIGNCTSLLWLNLADNLLTGRIPPEMAEIGRNPGPTFAKNRNDTSVLAGSGECQAMKRWIPASYPPFSFVYSVMTRESCRTIWDRMLKGYGIVPICTNSSSPVRSNTVSGYVQLSGNLLSGQIPSEIGAMRNLSLLHLDGNRLTGQLPAEIGRLPLVMLNVSRNNLSGPIPSEIGDILCIERMDLSFNNLSGELPASLFKLTELSMFNVSYNPLLSGNVSTTGQFGTFDEQSFLGNPLISLHQGGAAGKQQPPRPEAADAPGVRTGGIPRTIVMWLLFSLVIAFIAGTVVFAITSLRARFPVDQEPEPDSFSCEHSKGKYAFGLSSSPPSGSSSATGCSSSTEGVKVFRLDKTAFTYRDIVAATGNFSDDRVIGRGGSGVVYRGVLPDGRAVAVKKLSRPRDGVDGDSEREFRAEMEVLADRMGFTWPHPNLVTLYGWCLSGGAKILVYERLDGGSLEALICDTAAFGRAARLDAAVGVARALAFLHHECVPAVVHRDVKASNVLLDGEGRAKVTDFGLARVVRPGDTHVSTMVAGTVGYVAPEYAQTWRATTKGDVYSYGVLLMELATGRRAVDGGEECLVDWTRRTAKEGRKQQTEDQKTAGGTVSWELLALGMRCTADAPHERPDMPDVLAALLDIAGAANGDAGCAYCRSSEHSGGEQTSSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQK1</accession>
    <name>A0A8I6WQK1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123427201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044967125.1">
        <property type="nucleotide sequence ID" value="XM_045111190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQK1"/>
    <dbReference type="SMR" id="A0A8I6WQK1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180380"/>
    </dbReference>
    <dbReference type="GeneID" id="123427201"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0149080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0149080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0149080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427201"/>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="213"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="356" mass="39543" checksum="3B7916FD26AC7761" modified="2022-05-25" version="1">MDVGSCHLGGNADAVEFCPHRPFRHILAAATYTLQEQAGEEEEQQQDRAGTVSLFSVDAGAEDESQRLRLLHTVETAGVFDMKWSPTAPLLAQADAHGRLVLRRLEHEDGSDGGVVLTDVSAAKISSSMCLYVDWNQNADSLAVGLSDGSLSVIPMREDRLEVSEQWTAHQYEVWTCYFDRARPHLLYSGSDDCSFSCWDLRESPSNAVFRNKKSHTMGVCCIAQNPLEGNILLTGSYDEFLRVWDMRSMMKPVNEKSLNLGGGVWRLKYHPDIADVVLAACMHNGFAIVKTGAGDATIMETYCKHESLAYGADWQTSEAAEHNTNSSVIATCSFYDRLLRVWQPENLVNHPTFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WQZ5</accession>
    <name>A0A8I6WQZ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123425747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965398.1">
        <property type="nucleotide sequence ID" value="XM_045109463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WQZ5"/>
    <dbReference type="SMR" id="A0A8I6WQZ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0136700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0136700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0136700"/>
    </dbReference>
    <dbReference type="GeneID" id="123425747"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0136700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0136700.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0136700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425747"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF348">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0136700.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="19703" checksum="CD09E7CAB220D387" modified="2022-05-25" version="1">MAPAPASVATHMWTGSPYSFAVLERLLQKHFSVPRRLLQVHALLLTSGALSTSDAAATFPYNCLIHAHLRLPASTITPLCAPLRLFSAMLAAGAYPNGHTFPSLLRSASASGPATRSLHAQCLRHGLTADRFVACSLVSSYGRAGRLGCDASKVFDEIASPNLASSIAMLDVLCLRKVFCCSHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WUX9</accession>
    <name>A0A8I6WUX9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WUX9"/>
    <dbReference type="SMR" id="A0A8I6WUX9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0166980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0166980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0166980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0166980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0166980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0166980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0166980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="22621" checksum="722C1411247C95C9" modified="2022-05-25" version="1">MEFFMIILEKSSSRQVLPDNFVKMLDGHRPQNMKLRQAGNGLRKLWDVEVAFDTDGSMYLDRGWKQFVRAYDMWHGYFLVFRYDDNATFAVKVFDTTMCRRRYQDDDDASNGSRSSEYCDMCYVYGSSDSGYRKSSSDSGYSKSSSDDGLAMVIPQLSDRAMPILIEEYIPYPGLGLRRSKRIRMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WVJ9</accession>
    <name>A0A8I6WVJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WVJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0068400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0068400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0068400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0068400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0068400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0068400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48133:SF1">
        <property type="entry name" value="BOWMAN-BIRK SERINE PROTEASE INHIBITORS FAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48133">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0068400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="185" mass="22070" checksum="C37C8B5082990B2A" modified="2022-05-25" version="1">MASSGSMTRPPCADQEDMSNIWREAELDKIKEKDVPTPPCWCGDVCKVKVSTDRKNSWIEGRRFFVCPNYAHDHARPTNAYEVPLLPPPLCKYFTWIDHEVREHIHEDQYRDCMWRQHIFNESLARAEERERREEKEHNKRDDERKCKEKEARQEERARKLARAHAAQAEDEAHDKKGKWPRTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXB0</accession>
    <name>A0A8I6WXB0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXB0"/>
    <dbReference type="SMR" id="A0A8I6WXB0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0112540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0112540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0112540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0112540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0112540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0112540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0112540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15840" checksum="E1AC2E131E7EC8B7" modified="2022-05-25" version="1">MVLVSTSVGPRPVYDSDVEVCEPEIRTHIKILVEKLNLVEKENKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYVVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXW1</accession>
    <name>A0A8I6WXW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXW1"/>
    <dbReference type="SMR" id="A0A8I6WXW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332570"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF66">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0902">Two-component regulatory system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="142" mass="15880" checksum="0946CB79BD78A47A" modified="2022-05-25" version="1">MAAAALIARLNAHMESMYAEGTVDEDTFEELREDGTAAEVARQFIEDAYEILHDIDTLMEEPVVDFDEVEALTQQLMECTSSVGAQQVNLACMHFGNFYAIRCKEGCLVSLALVRNEFLIVRHELEIMMELEEQIAACGPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0B0</accession>
    <name>A0A8I6X0B0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0B0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0237030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0237030.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0237030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0285650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF114">
        <property type="entry name" value="RX N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18413" checksum="DA53ABD089E2FBB0" modified="2022-05-25" version="1">MDIIVPAVIGDIVSRTVSFAIKKYWRPPDVEKNLERLNQLVLRAHTIAEEAEGRLINNREMLQQLGILTEGMHRGRYMLDNFKCRILEEGKEDEQEVSQFPGLSMFNAAKCLRLSNNYRKAVLFGSNNIEDLQGMIATLEKAIGDLNEFVIFLANYPPICR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X599</accession>
    <name>A0A8I6X599_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123426088</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044965799.1">
        <property type="nucleotide sequence ID" value="XM_045109864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X599"/>
    <dbReference type="SMR" id="A0A8I6X599"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0146870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0146870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0146870"/>
    </dbReference>
    <dbReference type="GeneID" id="123426088"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0120910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0120910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0120910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0146870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0146870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0146870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426088"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000303">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000425">
        <property type="entry name" value="Serine/threonine-protein kinase SRPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF22">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="43"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="598"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="615"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0146870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="693" mass="76401" checksum="36E25BFD68ADE44C" modified="2022-05-25" version="1">MSRASLSPSSSGEEEEDDEGVDGYRKGGYHAVRPGDQFAAGRYVTQRKLGWGNFSTVWLAFDVESQKFVALKIQKSAPEFAQAALHEIEFLLEITKRDPSNCKCTIQLIDHFKHAGPNGQHICLVFEFLGDSLLKLVQYNRYKGIGLGRVKEICRSILVGLDYLHGELGIIHSDLKLENVLLVSTIDPSKDPIRSGLKPNLERPEGNPNGEAVLNPIDKKLKMRARRVLAKLAEKRKSAAEFARAERSLDGIDMTCKIVDFGNACWADKQFTDFIQTRQYRAPEVILGAGYSFPVDIWSFACIAFELATGEMLFTPKEGHGYSEDEDHLALMMEVLGKMPRKIATMGTKSKEYFDRHGDLKRIRRLKFSSIERVLVDKYKIPQSDAREFAEFLCPLLDFSPEKRPTAAQCLKNKWLQHDDGKNVTNIASKSIDVTCNIGSMPDSCAQRIDAKWNTNSSINVNTENADGVRPTERISNSNVKSTNVNPNTGTLMYKDGDNSDVKPHPGSIITNKNAKSSDEKPFTGSISNKDDKTVDTKPITWGSASSVDKIVDTTSSIGSVVNRDAKNIEGKRNIRSVVNSYMKNFDANRNTGSIANSEVKDLDVKPSSGDIASVDTNSTSAKPNTGSIENSDVTGTSAMANTGLAKSGSKLQTNTDSVDEDDIDSKPNVGRVAASIQRLESSMSKVQIGRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6I7</accession>
    <name>A0A8I6X6I7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X6I7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135880"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0111950.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0111950"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0135880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0135880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0135880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48133:SF1">
        <property type="entry name" value="BOWMAN-BIRK SERINE PROTEASE INHIBITORS FAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48133">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0135880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="87" mass="9998" checksum="554AC83DA0F4FFD0" modified="2022-05-25" version="1">MASSGSMTRPPCADQEDMPKKWMVAELDNTKETYVPTPPCWCGDVCKVKVSIDRNKEWTEDRRFFVCPNYAHDRARPTNAYDLPPVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X728</accession>
    <name>A0A8I6X728_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123443575</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975936.1">
        <property type="nucleotide sequence ID" value="XM_045120001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X728"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0259460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0259460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0259460"/>
    </dbReference>
    <dbReference type="GeneID" id="123443575"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0259460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0259460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0259460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443575"/>
    <dbReference type="OMA" id="MAQPYTT"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF39">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE LPEAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="15">
        <location>
            <begin position="164"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0259460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="40776" checksum="4F7879CF323ECCFC" modified="2022-05-25" version="1">MAVDTTTAAPSHPEGGDGGGEAVRPLLSGAPAEEEEEELDIRYAPYARRDAYGPMGRGPLSSAQVARLVLAGVVLLPLRLIAGMLLVVAYYLVCRVCTLFAGGVGEGRPRLQGLKREAVLRAGRVLSRAMLFVFGFYWIPVSDRSFPNAEDVPKDHSEELERPGAIVSNHVSYVDILYHMSASSPSFVAKNSVSKLPLIGLISKCLGCIFVQRESRCSDSKGVSGAVTERLHEVSQDENSSMMLLFPEGTTTNGDYLLPFKTGAFLARAPLQPVILRYPYRRFSPAWDSMDGARHVFLLLCQFANYMEVVRLPVYYPSEQEKQDPTVYASNVRKLLATEGNLVLSNLGLAEKRVYHAALNGNSPRALHQKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X770</accession>
    <name>A0A8I6X770_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123427829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967899.1">
        <property type="nucleotide sequence ID" value="XM_045111964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X770"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195910"/>
    </dbReference>
    <dbReference type="GeneID" id="123427829"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0162560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0162560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0162560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0195910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427829"/>
    <dbReference type="OrthoDB" id="678526at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF7">
        <property type="entry name" value="OS05G0160300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158829" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="132" mass="13999" checksum="8208AE53395692B3" modified="2022-05-25" version="1">MAMKPLVFLVLVVVLAVGIRAEEDSEGKDTGTHILSYLSMQAGECGRSSADRMALRMAPCFSAADDPDAQPTSSCCAAVHSIGRSPSCLCAVILSNTFKAAGIRPEVAITIPRRCNMADRPIGYKCGDYTMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8D5</accession>
    <name>A0A8I6X8D5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123440015</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044972543.1">
        <property type="nucleotide sequence ID" value="XM_045116608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8D5"/>
    <dbReference type="SMR" id="A0A8I6X8D5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0274630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0274630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0274630"/>
    </dbReference>
    <dbReference type="GeneID" id="123440015"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0227600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0227600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0227600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0274630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0274630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0274630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440015"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000173">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000503">
        <property type="entry name" value="Putative prolin-rich extensin-like receptor protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF66">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRICHEXTENSN"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="353"/>
            <end position="628"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
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            <end position="327"/>
        </location>
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            <begin position="671"/>
            <end position="695"/>
        </location>
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            <end position="33"/>
        </location>
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            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
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        <location>
            <begin position="70"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
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            <end position="151"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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            <end position="212"/>
        </location>
    </feature>
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            <end position="240"/>
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            <end position="303"/>
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            <end position="695"/>
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            <position position="381"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0274630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="695" mass="72493" checksum="87B2DE472BE2BCC5" modified="2022-05-25" version="1">MPRVLVESVQHLAAAPAAAAAGGLPAHPPQSSASPPPPAPTSPPPPTPPPKSPPPSSPPPASSGNNQSPNLPPGPGSPPPVKSPTSSPPPPASRPPSTPPTSRSPPPSSPPPPSPPSQQSSPPPAQSTPPSPGVSTQPSPKASTSRSQQSPPTSPPASPPPSQPKNPAPAIPTPSPPLNAESAPAGAGTSPPPLQTPVPAAAGQVPAAPGTPSSASLLPPPGIMPTSGPPPGTWQSPPRPAVAKASPPAPAPPTRSSAENLPTGVIVGVAVAGFLMALASLFMVACLSDRGKKRHPPMRKRRTTVVPARVPSPDSYPPSYGPAASPSGASSYEFSGYKSCFTYDELAGITGGFSADKVIGEGGFGKVYMGALGDGRRVAVKQLKVGGGQGEKEFRAEVDIISRIHHRHLVTLVGYCVTENHRLLVYEFVSNDTLEHHLHGQGLPVMDWPKRMKIAIGSARGLTYLHEDCHPRIIHRDIKSANILLDDAFEAKVADFGLAKLTNDAMTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDSSQPIGEESLVEWARPLLVDALETDDFREIADPALELRYSKTEMRRMVEAAASCIRHSGTKRPRMVQVWRSLDVDGGSTDLTNGVKLGHSTAYDSGQYSADIELFRRMAFETDLDSAEYGLSGEGEPRPGSGRQQRTAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9R7</accession>
    <name>A0A8I6X9R7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9R7"/>
    <dbReference type="SMR" id="A0A8I6X9R7"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0238740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0238740.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0287580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0287580"/>
    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:phospholipid binding"/>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ENTH_VHS"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF87">
        <property type="entry name" value="OS01G0738600 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="433"/>
            <end position="465"/>
        </location>
    </feature>
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        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
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        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="322"/>
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        </location>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
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        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0287580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="560" mass="59062" checksum="862323288C83EBB1" modified="2022-05-25" version="1">MIMKVLWQRLCNTDANWRHLYKALAVAEYLLANATERAVEEIIDNSPQIAKLTKFEFVEPNGKDVGLNVRKKAEAVLAIVDDREKLQQVQEKAASTRDKYLGVSSTGMSYKSSAASFGNGSYSSGSRYGGTAGSRATASFKDRYTGTELSNNNKPSHSSTRQRSKEPTKTANSSKSAKGGSKSLSSPRATPGVPSSQKGKNENDGDDFNPRGSSTSAGTANVSSNNLDLFGPSFMHGLVNTAAATSIAIPNAGSAAVPEIDLFADADFQSANAPLEAATSSGSRPKDNIDLFAIRPSFGGSATSDMEFSVCDSPKKHPEQKSSSLAQPPSASSFDPFNPSFATSFPLDTEFSVRGTPSKSSQGKPPTPENSSDTAFKPLAGIPVKSFSGSNSFGVFSSRTGSAVTQPTHGSAGASKSSYGSPLEELNFGAFTSDEESRTASVTKSMNESLAKQKQDSMSASKPAVKKETFRVKSSIWADSMSRGLIDLNIGAPKEDTSDIGAVGRLSNGSEEKAPAAVPWYMEAATGTTDLPRSTGTAGKTGIFQQQQHFGSFRKYGLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBK5</accession>
    <name>A0A8I6XBK5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123439467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972111.1">
        <property type="nucleotide sequence ID" value="XM_045116176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBK5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235550"/>
    </dbReference>
    <dbReference type="GeneID" id="123439467"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0195470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0195470.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0195470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0235550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0235550.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0235550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439467"/>
    <dbReference type="OrthoDB" id="593735at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235550.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="195" mass="21431" checksum="2160FD81E26F0037" modified="2022-05-25" version="1">MEEWRNLALAAADESFANTVTNGVAETIASAIQQYRLAAEECRGFGQGVHPTPNAGERASAGGDFVDLAIDRIKSISRFHAVRGSVFSLCVRRIGLQGNALWYMWQFYHADEARHAETAMLSLRSAKSHGHAAVRVFERMLRPPSPQAVARAWAPAAVQLLRRAIKNLAMAEVSVGQIRPAIVVEYNDARRLLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBP9</accession>
    <name>A0A8I6XBP9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBP9"/>
    <dbReference type="SMR" id="A0A8I6XBP9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="24425" checksum="19F1CACA08EA5415" modified="2022-05-25" version="1">MYDLYAMAHTASYKKVKAFSESDLEHPENFTNISSHDKLVKYRDHGKARKGEDFNPSEGPIDPELVMIAGNGRPHGSIAIGDGLIRCPSTLRQIKAHQTSSCPEITRRPRPVELAIEAAIQKEREAMQAAIAEKDKEIRELEERTTALMEAERARNDASNRAIYELFVSMCEKSGQVPPPMPVINVAGTNISRNASHDPSTVEPSPGQPSPGETSQSHRASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEV4</accession>
    <name>A0A8I6XEV4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123448233</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980997.1">
        <property type="nucleotide sequence ID" value="XM_045125062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEV4"/>
    <dbReference type="SMR" id="A0A8I6XEV4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332020"/>
    </dbReference>
    <dbReference type="GeneID" id="123448233"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0276630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0276630.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0276630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332020.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448233"/>
    <dbReference type="OrthoDB" id="1546383at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035340551" description="Germin-like protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="62"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332020.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="243" mass="24808" checksum="C698A4F039F5DE04" modified="2022-05-25" version="1">MAPSSLTTLLVLLAVVLVAHGSDPDILTDFVVPPGTNASLLDGTFFTYAGLIAGNSADPAKFTVSKATAAEFPALLGQSVSYAALVFGPGTVNPPHVHPRASELLYVVQGPLMVGLVDEIKRELYAQTLQTGDMFVFPKGMVHFQFNGGADVARAFSAFGSASPGTVSLPVALFESGISDAVLEKSFNVDEATVLALEHDLAPAAPAPAPAPDSPPAPKNGGAAPVPACLALMVGFAATLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEZ3</accession>
    <name>A0A8I6XEZ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxypeptidase</fullName>
            <ecNumber evidence="2">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123446906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044979384.1">
        <property type="nucleotide sequence ID" value="XM_045123449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEZ3"/>
    <dbReference type="SMR" id="A0A8I6XEZ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332980"/>
    </dbReference>
    <dbReference type="GeneID" id="123446906"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446906"/>
    <dbReference type="OrthoDB" id="591590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000573">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF462">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338746" description="Carboxypeptidase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="484" mass="53589" checksum="B442B7EB81C7542C" modified="2022-05-25" version="1">MKFAAAALHVLLFIPALALHARASQEAHLTKFLLSRTTSSTTTSDPSLHDKPFLEIISDLRTDEYPVSDPGAQKASDKITALPGQPEGVDFGQYGGYVTVDSHNGRALFYYFVESPDDAMAKPLLLWLNGGPGCSSLGHGAMRELGPFRANRDGKTLTVNKHAWNKVANVIFLESPAGVGFSYSNTSSDYDRMGDPMAAQDAYIFLLNWLDRFPEYKARPLYITGESYAGHYIPQLAATILAHNNNNKSNNNNNNNNNTINLKGIMVGNPYLDKMKNLIGHYEYLWSHGAISDEVWADIASSCSIDRSSINVRCYHAVIECQDGILDHDNIYGPTCIIDANGSYYSSTYLPGYDPCSYVPTKAYLNSPQVQKALHARETKWLDCRSFEDFKDSPDTMVPTLKRLINRGLPIWLFSGDFDSVCPFTGTRYTVRDLNLSVVEPWRPWTAGHEVGGYVQQYARGFTFATCSSPSSQGSFLHSTGNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFH2</accession>
    <name>A0A8I6XFH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="7">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123449718</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044982967.1">
        <property type="nucleotide sequence ID" value="XM_045127032.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982968.1">
        <property type="nucleotide sequence ID" value="XM_045127033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFH2"/>
    <dbReference type="SMR" id="A0A8I6XFH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408170"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408170.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408170.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408170"/>
    </dbReference>
    <dbReference type="GeneID" id="123449718"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0408170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0408170.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0408170.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0408170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449718"/>
    <dbReference type="OMA" id="FPYNTWG"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000285">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000265">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="71"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0408170.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="355" mass="40016" checksum="5AAA1A9F4F5FBD7F" modified="2022-05-25" version="1">MRGKKPLKELTLSVPAQETPVDKFLTASGTFKDGELRLNQRGLQLISEENGDEHQSTKMKVEDVQLSMDDLEMIQVIGKGSGGVVQLVRHKWVGTFYALKGIQMNIQESVRKQIVQELKINQATQSPHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAVLASSIGQRDTFVGTYNYMAPERISGSSYDYKSDVWSLGLVILECAIGRFPYTPPEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCSFISSCIQKDPAERKSASELLNHAFIKKFEDKDLDLRILVESLEQPMNVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFQ6</accession>
    <name>A0A8I6XFQ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain dehydrogenase/reductase</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123427337</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044967288.1">
        <property type="nucleotide sequence ID" value="XM_045111353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFQ6"/>
    <dbReference type="SMR" id="A0A8I6XFQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0184020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0184020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0184020"/>
    </dbReference>
    <dbReference type="GeneID" id="123427337"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0152300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0152300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0152300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0184020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0184020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0184020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427337"/>
    <dbReference type="OMA" id="WMWKNCR"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000396">
        <property type="entry name" value="(+)-neomenthol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF78">
        <property type="entry name" value="OS04G0532100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0184020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="307" mass="33493" checksum="AACDAC53CE03C8A3" modified="2022-05-25" version="1">MEGSTPTITRIAVVTGGSKGIGLEACKQLAGSGITVVLTARDETRGTAAVEQIKRLGHSDVIFHQLDITDASSIARLADFLKTRFGKLDILVNNAATDGIEHVINPVYGLIPGDETFNGMDAGQRMDWMWTTCRQTYETAKQGLQTNYYGTKRVTEALLPLLRSSSDGRIVNVSSNFGLLSFFRNEELKKELNDVDSLTEERLDELLAMFLKDFEAGAVEAHGWPAEFSAYKVAKAAMNAYSRILAKRHPELRVNCAHPGYVKTDITRNSGILTPEEGARNITGVALLPEGGPTGKYFSEGEEASFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHJ9</accession>
    <name>A0A8I6XHJ9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123449474</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982647.1">
        <property type="nucleotide sequence ID" value="XM_045126712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHJ9"/>
    <dbReference type="SMR" id="A0A8I6XHJ9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400930.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400930.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400930"/>
    </dbReference>
    <dbReference type="GeneID" id="123449474"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0333640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0333640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0333640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0400930.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0400930.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0400930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449474"/>
    <dbReference type="OMA" id="IRMPNDN"/>
    <dbReference type="OrthoDB" id="8123449at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990446">
        <property type="term" value="F:U1 snRNP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12283">
        <property type="entry name" value="RRM1_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12285">
        <property type="entry name" value="RRM3_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000263">
        <property type="entry name" value="Splicing factor CC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000584">
        <property type="entry name" value="Splicing factor CC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000276">
        <property type="entry name" value="Splicing factor, CC1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029123">
        <property type="entry name" value="RBM39_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006509">
        <property type="entry name" value="RBM39_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01622">
        <property type="entry name" value="SF-CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48036">
        <property type="entry name" value="SPLICING FACTOR (PAD-1), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G15810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15519">
        <property type="entry name" value="RBM39linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="175"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="272"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0400930.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="543" mass="61218" checksum="850566FE094C9771" modified="2022-05-25" version="1">MDFDEYEYLEKTVEASAPAPANGSEGKERSSRRRSSAGDGEDRDDGERRSKRSRSEHRDRDRDRERDRDRDPESRERHRSSRERRDRDRDRDRDVREKERLSREGERERRSRDHDREREKEKERERRSRSRSERRRDDDERERERFRERDYRDRDVRRRKEDGAEPEADPERDQRTVFAYQLSLKADERDVYEFFSRAGKVRDVRLIMDRNSRRSKGVGYIEFYDVMSVPMAITLNNQLLLGQPVAVKPSEAEKNLVQSNASSSVAASGGARKLYVGNLHANITEEQLRLVFEPFGLVELVQLPVDPLTGLCKGFGFVQFARLEDAKAAQSLNGQLDIAGKVIKVSAVTDHVGAQASGAAPGDLDDDEGGGLALNASSRALLMQKLDRSGATTSLTSGLGASAVALPQPASVLGAPAAGTALQPMVPGLGLIPGASVPVITQTIDMAPPSECLLLKNMFDPAVETDPDFDLDIRDDVREECSKFGQVRHIFVDKNTAGFVYLRFDSITAAMGAQKALQGRWFAGKMITATFMSIQQYEMKFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJG4</accession>
    <name>A0A8I6XJG4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123399738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    </reference>
    <reference evidence="8" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Triticodae</taxon>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
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        <reaction evidence="13">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
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            <name>Zn(2+)</name>
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        <property type="entry name" value="ADH_Zn_CS"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="16">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
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        <location>
            <begin position="145"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0252910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="332" mass="36166" checksum="846B3119D48E9BDC" modified="2022-05-25" version="1">MFPRIFGHEAGGIVESVGEGVTDVAPGDHVLPVFTGECKECPHCKSAESNMCDLLRINTDRGVMIGDRKSRFSIGGKPIYHFVGTSTFSQYTVMHVGCVAKINPEAPLDKVCVLSCGISTGLGASINVAKPPKGSTVAIFGLGVVGLAAAEDARITGASRITCVDLNASRFEEARKFGCTEFVNPKDHTKPVQQVLVDMTNGGVDRSVECTGNVNAMIQAFECVHDGWGVAVLVGVPHKDAEFKTHPMNFLNERTLKGTFFGNFKPRTDVPNVVEMYMKLCWRSCPPTTARGRRSFRPQRTKHAMRNASVGDDHEFYLSKFKDRVDRTLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNQ3</accession>
    <name>A0A8I6XNQ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNQ3"/>
    <dbReference type="SMR" id="A0A8I6XNQ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0535200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0535200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0535200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0445320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0445320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0445320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0535200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0535200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0535200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="4">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0535200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="429" mass="46130" checksum="A9595C0BA5848853" modified="2022-05-25" version="1">MAGLNTTLRQLVGLLPEVADRRATPVPFRDKALNTVTSVLVFLAGSRLPLYGLSTSAAPDPFYWIHAASASNCGSIMAQGIYPLLASELIIHPLFGLKIVRDKIPMQERTQFMYRSQKLLSMLIAMASAVCNVFSAGQLGLVDATMIALQLFAGGAMVIYLDEALKKGHGLVSSGIALFTATNICACLLETYGADKMVITAGTCAFLALTVRLQGSHLPLAVRTHGIEPALQANCSVNLPYLSYMPIVFQGTIASSMYFISRLLYLKYGETDIGVVKLLGSWKRDSRYPGQPIPTGGVARYVTNPPTWSDLARDPVHVTLYAALLLMGCALISAAWFGTRVHSREYMARLLGRQIPVTPAQPGSIPTNRWKRHVAMIALLVGLCVGALTLVAGFIGVPGSGTGIMLVVTVLCSYFERRAGDEAADVFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPX4</accession>
    <name>A0A8I6XPX4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123439355</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WUS homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044972016.1">
        <property type="nucleotide sequence ID" value="XM_045116081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XPX4"/>
    <dbReference type="SMR" id="A0A8I6XPX4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0230550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0230550"/>
    </dbReference>
    <dbReference type="GeneID" id="123439355"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0191290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0191290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0191290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0230550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0230550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0230550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439355"/>
    <dbReference type="OMA" id="CGYMAND"/>
    <dbReference type="OrthoDB" id="670226at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048830">
        <property type="term" value="P:adventitious root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010311">
        <property type="term" value="P:lateral root formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000118">
        <property type="entry name" value="WUSCHEL-related homeobox 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044558">
        <property type="entry name" value="WOX11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46998:SF1">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46998">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="25"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0230550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="261" mass="28212" checksum="D187518961F8325A" modified="2022-05-25" version="1">MEQHHHGQAPTPTHRGGRSSEGGEPTTTRSRWAPKPEQILILESIFNSGMVNPAKDETARIRLLLERFGAVRDANVFYWFQNRRSRSRRRARQLQQSCGGTGDADQLPSNAVLAGHGYHGIGTSPYNAMQYGQLCGGVPAAAVTAAPHFLVDDADSGDDLFAIPRQMGLMARGGESQHSYTATDASQLSYQATVPGTTMPVFINGTVYDVPSTGALDVAGTFGSDVILVHSSGEILPVNERGVLMKSLQMGECYYLVFRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSN3</accession>
    <name>A0A8I6XSN3_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSN3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503130.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0503130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0503130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0503130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0503130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15704" checksum="C9B841E7DB3BBCCF" modified="2022-05-25" version="1">MEFHDPTFQAPGTRPKRMYPAGVVVENQLRVWAMSRWRGGREFAGLFLRALYRHLPVGTPQMFTIDESRMGGTGMIVRFRNPYDAYHLLGRVWFGGCEFICFTTYNIFTDLDSIWPNEHAMHTLPYNLPPADDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XUJ7</accession>
    <name>A0A8I6XUJ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XUJ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592000.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0592000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF14">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="106"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="304" mass="35751" checksum="556E5D738D1CC6F3" modified="2022-05-25" version="1">MKIKMTFFQKVKFIVGNGTSTRFWKDTWLGDTPLALQYPLLYNIAQRKEDYISMVLQSVPLNMQFRRALVGARWDAWLHLVRRLMQVQLSNHTDNVRWILAANGVFSVKSMYLDLINTGTLSRSLKIWNIKVPLKIKVFMWFIHKGVILTKDNLIKRRWRGRSNCCYCEQNETIRHLFLQCPLAKLLWRTIQIAFNIVPPTCISSIFTTWLNGVDVKISKLIGIGICALFWAIWNTRNDITFNGKNFNNFLQVIFRATSWIRTWSLLSHVDSRGRMDIGCNRWEMVARVIFNRFGWRANNRLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVT3</accession>
    <name>A0A8I6XVT3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVT3"/>
    <dbReference type="SMR" id="A0A8I6XVT3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530610.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530610.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15737" checksum="3CCD86AA528C7034" modified="2022-05-25" version="1">MVLVTASVGPRPVYDSDVEVCEPEIPTHIKILVEKLNLVEKENKYLKKKLKIIEDKNMELELHVADVIDDRKMKIDAMRLKMDAMRLKMDEMRLKIRNIRKYAIDKEAWYHYAVGSIVTLVAILIAFVVAFKCFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWN1</accession>
    <name>A0A8I6XWN1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fungal lipase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123398421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948829.1">
        <property type="nucleotide sequence ID" value="XM_045092894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWN1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0512270"/>
    </dbReference>
    <dbReference type="GeneID" id="123398421"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0425530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0425530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0425530"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0512270.1"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="581375at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31479">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31479:SF42">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0512270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="40270" checksum="58C51B468A1D3427" modified="2022-05-25" version="1">MDPNTPDRFDADELKHIVRAGSSPAKIDWGNEEHRRCIVACMVKGAYVLESDRAMGRTEAASLAPAWWESFHFRLHGELTEDAAIYGAIYEHVPGPRHPSAPLYVVAFRGTMPNHPDWAVVMKDLYLDFKIVINKLKKRTRSKLACGAVDELMDGQASLGSADVWLAGHSLGASLALDVGRDMMEKRELNLPTFLFNPPQVSLTAAVNFVKAQDVAKRHLHFTSYFFKAGAATVSGCHRERMEKLFDRLSPWVPNLYLHDKDWICQGFIDYFELRQRFLDRFPRVASKSMTLSYRDMLWRLLGKDKERPHLLPSAMLWKTSQISLGAHGLQQWWKPNAELGLGAGTRYSYPVPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XXK6</accession>
    <name>A0A8I6XXK6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123451189</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984134.1">
        <property type="nucleotide sequence ID" value="XM_045128199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXK6"/>
    <dbReference type="SMR" id="A0A8I6XXK6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345170.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345170.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345170"/>
    </dbReference>
    <dbReference type="GeneID" id="123451189"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0288140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0288140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0288140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345170.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345170.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451189"/>
    <dbReference type="OrthoDB" id="684508at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF35">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="240"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345170.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="359" mass="37713" checksum="9BBCD2E62D36D6F9" modified="2022-05-25" version="1">MMNLISFETPPPLGRQQSTAATAVTGMKVATEASSSGACFHGSNLDLSLGISLSPGGNCSAAICGDSVMASYKGRPGDGNVGRVQGSGLVVASTATANTLSTGRDGNCHGIVPSSSWTAAFMPSPTGFMHPWSLAARQQKAAAEQDRSIAPTSVATTYVTSDASVVSLPSAAVGWPPVHTSRRHLVTAVPKPDADVKQPDGPKDGRIPAAASPGADDDKEVAGAPRSCTVAVEPQRLPANMFAKVQMDGCLIVRKINLRAHRSYDSLSRALTKMTRNFFCPADYQNSDSGEGDCANSDNFIFLYEDFEGDRMLVGDVPWELFLASAKKLCIARHPTARDTKGHDEAIKLDASVKLSTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYH7</accession>
    <name>A0A8I6XYH7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYH7"/>
    <dbReference type="SMR" id="A0A8I6XYH7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0352620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0352620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0352620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0352620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0352620.1"/>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF11">
        <property type="entry name" value="DUF4201 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0352620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="224" mass="25499" checksum="64D4E1F1C0CD9818" modified="2022-05-25" version="1">MVYEDESSNELSSLDISSSSDGMMYRIPASIDDEDYMGIENSVLDVCLEHGLPPERRVAFEGFETGRRFLVCAQPQERVMDLSYMKVNAHGAESSSSVVSVMQSEIDKKEAEIMEVKGKYSMLMNLAESQGRVIRTQKANHLKEKEKLSEENYNLKVQVDKLTKSEDKLNDDIVVLNLHIDGLKKGIQNLIKRRDELKLQIADQLKALEKNEEKLKMIRDILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZC6</accession>
    <name>A0A8I6XZC6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZC6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0373350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0373350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0373350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0373350.1">
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        </location>
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        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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                <person name="Brown J.W."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
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            <authorList>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28944" checksum="A0ED614116FD8BB1" modified="2022-05-25" version="1">MPARPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDKDARALRIELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPLVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0580960"/>
    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0580960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="76" mass="8992" checksum="A5E181D8E263F71A" modified="2022-05-25" version="1">MQPSLFFIQLFSNGNVFQGDWKNRSVTGTSDRENTLWPMATSVWRERLAGKKQDFWRDHINNFHVNLNIKKCIYSA</sequence>
</entry>
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    <accession>A0A8I6Y3R7</accession>
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        <recommendedName>
            <fullName evidence="5">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
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        <name evidence="6" type="primary">LOC123452879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3R7"/>
    <dbReference type="SMR" id="A0A8I6Y3R7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0522670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0522670.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0434530.1"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Histone H3"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF00125"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
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</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="486" mass="55799" checksum="414F74DC11766186" modified="2022-05-25" version="1">MDHQHHKAGLRGRLRVTAARRRAWQQQSAACCKRPARRDPVDTVRKFMRREIGGGHRRPPRPAAPSTSAFSCPEKFRNFQLQEEYDTYDDPFVQLPLGWNRRRIIEIVAARDLIFALAECGLCGAFNRTTNKRICHLNISPDEVIRSLFYNKNNDSLITVSVYESDRYSSLKCRTTPIEYIRRGQLHDGFPLFETESLKYPGFVEFDDVNGKVLTFSAHDSTYKVFDLKNYNFLYSICDKDIQEIKISPGIMLVIYQKASNHVPLRILSIEDGTPLKTFTQLLHRNRKVDFIEQFNEKLLVKQDKENLQIIDVRNSGLIEVNKTEFMTPSAFIFLYENNLFLTFCNRTVAAWNFRGELVTSFDDHELWHPNCNTNNIYITADQDLIISYCKVSRGGTNDADDEGREGSCMGSINMSNIFTGKCVAKISAFDPTLTIAPRRNGDTSRSTIRSSVSDALEDITALFYDEDRNEIYTGNSRGLVHVWSN</sequence>
</entry>
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    </protein>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pooideae</taxon>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0431430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0431430"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0431430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0431430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0431430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="14323" checksum="E4FE88ADFF24E7B2" modified="2022-05-25" version="1">MYYRWIDTEMLDWAVIEIRERGHRAWASLDLEERRAKAEAEEKAEQKKEWEDYCVEQRAFIDEMIRKNQEEKLRLEEVYRQREQAHEAERGRKREMARAAKAAEEAGDGKGKYPCWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6W1</accession>
    <name>A0A8I6Y6W1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <end position="228"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0373870.1"/>
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    </evidence>
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    </evidence>
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</entry>
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        <lineage>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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</entry>
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        <lineage>
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            <taxon>Poales</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16">cv. Morex</strain>
        </source>
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    <reference evidence="15" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
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            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045091981.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0465890.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0465890"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
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        <property type="entry name" value="Ntn_hydrolases_N"/>
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        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATASE_TYPE_2"/>
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    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="341"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0465890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="585" mass="65527" checksum="DC64C85DCB4702FE" modified="2022-05-25" version="1">MCGILAVLGCADDTQGKRVRVLELSRRLKHRGPDWSGMHQVGDCYLSHQRLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEQLRAQLSSHTFRTGSDCEVIAHLYEEHGENFIDMLDGVFSFVLLDTRDNSFIAARDAIGVTPMYVGWGIDGSVWISSEMKGLNDDCEHFEIFPPGHLYSSKEGGFKRWYNPPWFSEVIPSVPYDPLALRKAFEKAVTKRLMTDVPFGVLLSGGLDSSLVAAVTVRHLAGTKAAKRWGTKLHSFCVGLEGSPDLKAAKEVANYLGTMHHEFTFTVQDGIDAIEDVIYHTETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEELHRETCQKIKALHQYDCLRANKATSAWGLEARVPFLDKEFINEAMSIDPEWKMIRPDLGRIEKWVLRKAFDDEEQPFLPKHILYRQKEQFSDGVGYSWIDGLKAHTESNVTDKMMSNAKFIYPHNTPTTKEAYCYRMIFERFFPQNSAILTVPGGPSVACSTAKAVEWDARWSGNLDPSGRAALGVHLSAYEQEHLPATIMAGTSKKPRMIKVAAPGVAIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y887</accession>
    <name>A0A8I6Y887_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123399024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="202" mass="22007" checksum="2F18FB5ABC91C627" modified="2022-05-25" version="1">MLARRSTSDLSSFQGIDSASVPASPLPDGAGGGTPVRFRMPLPKAQGEKLMGESRDAAEAAAKIMELCTAVGDGGCSAKVPPEKPPFILRIPRFATTPEWGSGFMLPKPDGRRSSRRGFCGAPRCRRLLFAAELYSPPSASGSGCPRILPCHCRFPSLPPRPALVALLDWHVGGIRSWSLFRHQHLYCYLELVERQLEKLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y8A3</accession>
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        </submittedName>
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        <name evidence="2" type="primary">LOC123441127</name>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
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                <person name="Brown J.W."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        </source>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0327620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="127" mass="13202" checksum="24657DF12FA0700E" modified="2022-05-25" version="1">MAPIKNIASALILIVAMVATASLPMSSAVVDRTDEAALGELTPIIKTALDGVIAASPPSKVFEVKSDVVNQELVAMATMNMAQGDKEKLSAYIKAYKTAAQLVTDAAPAEKLTVMDDAFTKAANPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y924</accession>
    <name>A0A8I6Y924_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">Apyrase</fullName>
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    </protein>
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    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045100553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y924"/>
    <dbReference type="SMR" id="A0A8I6Y924"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651330"/>
    </dbReference>
    <dbReference type="GeneID" id="123407420"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0651330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407420"/>
    <dbReference type="OMA" id="HFANAPQ"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF81">
        <property type="entry name" value="APYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035225564" description="Apyrase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="47175" checksum="E29D6464DE5AA371" modified="2022-05-25" version="1">MAHVVEGIVALFLILLAASVEIALARAATGPPAGKYAVILDAGSTGTRMHVFRFDKRMDLVKIGDDIEVFAKVNPGLSSYAGRPKEATKSILPLLQEANSAVPQRLMKTTPVKLGATAGLRLIGDKQAEQILDAVRGAVHTKTKFQYNPKWINVLEGSQEGSYLWVALNYLLDKLGGDYSKTVGVIDLGGGSVQMAYAISPTAAAAAPQVPHGKDPYVTKEYLKGRDYNVYVHSYLRYGALASRVEIFKAKEGPFSYCMLRGFSGKYTYNGEEYNATASIGGAQYGKCRGDVVKALKLDVPCQAKKCAFDGVWNGGGGPGQANLYVASSFYYMASQVGLIDSDAPSGTSTPAAFKAIAQKICRMSMKEVKAKYPKVRDIPYVCMDLVYQYSLLIDGFGLEPTKKITLVQKVKHGEYFIEAAWPLGEAIEAVAPKKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y9U8</accession>
    <name>A0A8I6Y9U8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123447015</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979504.1">
        <property type="nucleotide sequence ID" value="XM_045123569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y9U8"/>
    <dbReference type="SMR" id="A0A8I6Y9U8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333640"/>
    </dbReference>
    <dbReference type="GeneID" id="123447015"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0278110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0278110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0278110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0333640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0333640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0333640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447015"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000687">
        <property type="entry name" value="Ras-related protein RABF2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0333640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="204" mass="22154" checksum="F8EF9F2F516A5257" modified="2022-05-25" version="1">MAASAGSKIRNAKLVLLGDVGAGKSSLVLRFVKGQFVEFQESTIGAAFFSQTLAVNDETVKFEIWDTAGQERYHSLAPMYYRGAAAAIVVYDISNQASFTRAKKWVQELQAQGNQNTVVALAGNKADLLEAREVQIEEAKTYAQENGLFFMETSAKTATNVNDIFYEIAKRLLQGQAAQNPQAAGMVLSQRPNERVVSAASCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBQ7</accession>
    <name>A0A8I6YBQ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123412965</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044961851.1">
        <property type="nucleotide sequence ID" value="XM_045105916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBQ7"/>
    <dbReference type="SMR" id="A0A8I6YBQ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653350"/>
    </dbReference>
    <dbReference type="GeneID" id="123412965"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412965"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF36">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035326019" description="chitinase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="27"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="298" mass="31466" checksum="BE10F0DC254B8B3B" modified="2022-05-25" version="1">MASRSSSLLQLLVVAVAAAQFLGSEAGGISIYWGQNGGEGTLAETCATGNYKFVNLAFLAAFGNGQPPVLNLAGHCDPTNGGCANLSSDIKACQSRGVKVILSIGGGAGSYYLSSTQDAKNVATYLWNNYLGGKSSSRPLGDAVLDGIDFDIEGGTPLHWDDLARFLKGYSNSGRRVYLTAAPQCPFPDAWVGGALNTGLFDYVWVQFYNNAPCQYTSGSTTNLADAWKQWLTVPAKQIFLGLPASPQAAGSGFIPADDLKSDVLPLIKSTGKYGGIMLWSKYYDDQDGYSSSVKSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBR1</accession>
    <name>A0A8I6YBR1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBR1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0676550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="37644" checksum="A05B2C712A1F12EF" modified="2022-05-25" version="1">MIVNLSSSSCRRKDMLFDKQKEVLLAKIEGGEMPTGRGKNQETSLVRPGDTRWGSHYRTLSHIESMWDAVIEVLGIVEDDVRVPSREGGLVHQMETFSFVFILKMMLKLLCMTDDLSLLLQKKDQNVVQAMSLVMDVRTRLINWRNDGWEPLLEDVKAFCTKNDIPIPNMDDMFTKWGKSRKGGRNNVTADHFFHVDTFYVAIDSITTKFDHRFNEVSSEMLQNFSCLDPRNSFSRFNVVKLAQLTEIYHADFSNYECEHIEYNLELFIIHMRRVEDFRACHDIASLAKKMVELDIMFPTIYRLIELALLLTVATQRLKGPFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YC56</accession>
    <name>A0A8I6YC56_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123398411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948820.1">
        <property type="nucleotide sequence ID" value="XM_045092885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YC56"/>
    <dbReference type="SMR" id="A0A8I6YC56"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509350"/>
    </dbReference>
    <dbReference type="GeneID" id="123398411"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423020.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398411"/>
    <dbReference type="OMA" id="FHHTLTE"/>
    <dbReference type="OrthoDB" id="1859279at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0052">Apoplast</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="176" mass="17860" checksum="5597FA1D7A8F1824" modified="2022-05-25" version="1">MLGRVVFCVVIAAAVLAVVLLATVSPLPHRSAARPRGITVYVHPVASGPVRRQQGAKHGDERVASALVFRHRMTAGPEITSRTVGAASGFVLPGERGSAMSAFDTVHLAFDAPGLSGSLCVEVARNEGALRVVGGTGAFAFAQGHGAVLRPERLGGGAAATSLRLELSMSVASAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGT3</accession>
    <name>A0A8I6YGT3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGT3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0398190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0398190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0398190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480460.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="142" mass="15971" checksum="6C6DE26B3D3F5CEE" modified="2022-05-25" version="1">MCLCERNRACRGRVASRIFQSPDRISSLERRDVPIGACVRTRPPSARFPAARRRSARRPCRVVGEGNSRSTSAATGSMERPRPWPWTDMTVCLRMVNSPRRRRAAGRYRVSAGRRCIGTTAGREMLERRRVGKLGTTGTRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YJS6</accession>
    <name>A0A8I6YJS6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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                <person name="Mascher M."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="3">subsp. vulgare</strain>
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530670.1">
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        <location>
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            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="268"/>
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        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="385" mass="42673" checksum="1BE1007E04E3B8C6" modified="2022-05-25" version="1">MRNQRIAPISCSSSKTNNNKQEEPHLSGAYIRSLVKHLSSSSTARSRDHHHITMGTKSYQEEQQGSQTTPPSLQQQQPHKKQVRRRLHTSRPYQERLLNMAEARREIVTALKIHRASMREAKEQQQQQQLVQQLQHQQEVQVVQDNRVAFSAPGMSSYGPFSDYLHSSSPFAYSTSTSNSGYSYYSSPPLLPYHTPVVVPMLPMVDALDQLVPLPTQPLGLNLTFDGFGGGVAGEDANNFTATSSSFDPPSLVQQPSPASSYSVYSSPPPATMVSQDMASAAAENTSQSLHRVLDEEEMAAIHSAGERHDIEWSDTVNLATSAWWSRLLESVEHGEGDDGATVAQETCKADSMGMHLSDEYCRQDVSFPCMDIGEIEGWNADWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRU9</accession>
    <name>A0A8I6YRU9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRU9"/>
    <dbReference type="SMR" id="A0A8I6YRU9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0599100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0599100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0599100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0722420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020">
        <property type="entry name" value="OSJNBB0059K02.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020:SF14">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035245849" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="39721" checksum="CBFAA3B3388941AF" modified="2022-05-25" version="1">MKLLPAVLCLALLLLVLNPNGVEARPAPTGGHQKKPSSAIFFVFGDDFADNGNLPLTDPVTEMSRQWAYPYGSDYVDADGHPRPNTPSGRFSNYQIQSDFIAIMLGLEEAPPAHARTAEKTCDPSGMTFASAGAGVLGSTSQDVPTLSKQVDTFRKMVKDGTISEQQLSHSVALVAISGNDYASASVIGLGSPNDINAYIGKVTKEIATNVEQLLKLGVTKVLVNNLHPVGCTPSQTRTNNYTACNIFGNLGASIHNDNLKQVMTTKKNVHIIDLYTAFTNIVDHAPGKGSELSKQFKRKLSPCCESLDSKGYCGQHGESPSELLFTVCEKSNKFFYWDDMHPTHAGWDVVMKQLEKPLREFVNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YS78</accession>
    <name>A0A8I6YS78_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YS78"/>
    <dbReference type="SMR" id="A0A8I6YS78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0741270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0741270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0741270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0614820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0614820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0614820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0741270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0741270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0741270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF562">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152956" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0741270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="40580" checksum="291A55ED5684EFC0" modified="2022-05-25" version="1">MGSSNRHCSISLAVVFVLAVLLLNADLGWCGCFKRIFSFGDSITDTGNFAYISHNSPPGAPSVPPYGETYFHRPTGRASDGRLIIDFYAQALGLPLLPPSIPEEKTGKFPTGANFAVFGSIALNPKYFMSRYNFSMQHGCLDDQLASFNRVLARIAPGDAATKSLLSESLVVFGEIGGNDYNFWFFDHTRSRETAHKYMPDVIARIGAGVQEVIKLGAKTILVPGNFPIGCVPVYLSGNKSYTATDYDQFGCLKWFNAFSQMHNQLLKQEISKLKSQNPGVKIIYGNYYGAFMEFIKNPSRNGIDKPLVACCGGNGPYGTGHVCDQKAKVCPDPSRFANWDQIHMTEKAYNVIANGVLNGPYADTPLLNAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YV54</accession>
    <name>A0A8I6YV54_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YV54"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0566600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0566600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0566600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0683280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF932">
        <property type="entry name" value="USP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="446"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="830"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="916"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1089" mass="119637" checksum="55DB486A879F71E5" modified="2022-05-25" version="1">MVEEKRERAEKASESPQKSPRLDPLAAPAAGPGCSATSASPTESSEWPSYEEAEAATSSDSTVDSMDDSGRCRHILYDLEMVVQDLKVANKVLKDPLRCEHRDCKTTWKGGGEDDQGMMKCIDCAYFFCSGWPVDRENPKGHAGWHAGGHQHWVAQWCDEPNLGYCFLCERPMRLSDCSEDDYAVVARNEKDQQAPGDSVAKDGWGTMASIAKDACETGAENPQKPPGMDLLLLASAAENTQKYLHLGLPASEAPVELSESPSTGEAEETSDCTYDIGSCDHLFKDREELDEMACAIKTAEKPPQCEHYLCTTTCTWRGAAAGLMVCTECTSTFCTGEKGDMENPQGHARWHATRDYHWVALWRDEPYKGYCFKCGHVLKLGQDKLSDDEWATLTRNKRDERAMPGSNGWNAWGTVVTESYAKDFWPMVTGDDASGYANGNGCVIRGLSNLGNTCYMNALLQCLLALGELRTTILGPGARLGSIGLHLKQLFVETSSTNNSRRALEPKMLLEDMRVFNPQFRGSFMQDSEEFLTSLCSALEEETDDLNKLHGGVKFPIIGDSIFGVWLRQTVCCISCSTESVSDMRIHELQLALPSKDCPARSVTLPPKTRSHGSPTKTRKELFQQTDKTDGEKIQTIAEGGDSQFPGSEFGDEAMVKTPKPLQVDSTEVEDVAHGRLQTQKNDFPLEIIEVPIKVLDFIPNLFDDTEGMDESIVDPHSPEDTGPPPLVDIEAKENAYSVELTTEDKGRAQSSDITDDEAVRMNSVASLEDCLTLFCYCPVEWKCDKCSEVVKLPRTNGSENGEPMIASTNVNPTVEGDRTELSDRKTCPSEQSNDSNNLSVECTSPSRQPHGSDAQNQVSISEDRISEEITSGMSYDEKNSASCSTTNKKPESHEGVQEAAPSSFPTDKQTDLLGTQDSQDTSDQNQGSGKQVKLDDHSAQQVEENQMEQKHGTGGFQTQLVTKLPPVLTIQLKRFTNALSKTRGHVSFKEILHVGPFVDPSSEDKGNSSYRLVGVIEHRGHALNVGHYVAYVRAGPKQQSSGSSSWVHASDCAIKEVPLKEVLGCEAYMLFYERMDGRGRSGGLPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z063</accession>
    <name>A0A8I6Z063_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6Z063"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0565030"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0468330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0468330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0565030.1">
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="59"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0565030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12950" checksum="44377183B8306E40" modified="2022-05-25" version="1">MEKMKDAPIPEGEEPKSDAEIVEEVLKTEVNQSTFLRNVGLKSSSKNSGKGTAVVAAHVRDLQQKLERSELQAEVMQEEMEAIKLKAKEYEATRDKELELLRKKSQEHEEKLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z2Y8</accession>
    <name>A0A8I6Z2Y8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z2Y8"/>
    <dbReference type="SMR" id="A0A8I6Z2Y8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0696530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0696530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0696530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0696530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0696530.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0696530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF5">
        <property type="entry name" value="OS08G0393150 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="hAT-like transposase RNase-H fold" evidence="3">
        <location>
            <begin position="286"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="454"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0696530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="62866" checksum="FB5257C20D04B747" modified="2022-05-25" version="1">MVKFCIHAEIPFLKFEDPHLQPWIDSLQPAFQIKGRHTIHDDALKMYKGMKKDIEVELQNLDSRICLTSDMWTSSQNLGYMSITAHYIDAEFNYKKKIISFTDVKYPHTGYAIEEEIVGCLTEWGIRGKLFTLTLDNASNNTNACHELIKYHKHELLLEGQHLHVRCCAHILNILVQDGMKIIHGAIDKIRELLKYIDSSSSRLQEFNTIAIGMGLPSKKGISIDTPTRWNSTWKMLVQGLRYKSVLTSYANRKMIESPSEEEWLRAEAICDFLKAFEELTLIVSAHRKPTAHKFLPVVLCIRHALKDPGWQTSDVLKELAAAMQTKLDKYWDPEEKENADPNRRRKSKDIAFNHALVIATFLDPRRKEDYLDFFYCKLSTNEEEVSNQVEVALEWVRKYFKEYELLVATSTAHSTPSSQGNTTIGSPIVGKRKLEEEFAQHKSHRRTRVLKSELDAYLEEASEEDGADFDVLGWWKRHAEKFPILATMARDFLSIPLSTVASEAAFSCGKRILGDSRSSLTPHMLEALVCAKDWLFIPKKGEFYLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z373</accession>
    <name>A0A8I6Z373_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
        </source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
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    </dbReference>
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        <location>
            <begin position="121"/>
            <end position="139"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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    </evidence>
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        <source>
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        <lineage>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="FHA_dom"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="554" mass="59753" checksum="B726755B38A0ED94" modified="2022-05-25" version="1">MAAPPPVLTLAVEKGPREGETRRCRVGAALRVGRIASGNDLAVRDVGASQRHLTIEFLPPPASRWAVSDLGSSNGTFINGARLLPSVPAPLSHGDLIILGQSTVLAVSIAPDSDVKPGPRRSSRRAAAVAVAEEKPTPSVTRLSTRKMLGTRAAAAPSETEKGGPGTAAVVVEEESHLVVTRGGGRKKVAGAEPLGVGKEEMEEAAVATLRGKRKKVVEFPELEKADEQKEEKAAVATRGRKRKKAVESPELEKADGRKEEKAAVATRGRKRKNSVEFPELETADEQKEEKAVVATRGRKRKNSVEFPELETTDEQREEKAVVATSGRKRKKAEPSEPEKGDEAVLVTCRDGREVATTVAPPPLPPKTRVQGRVTRARARKAVLEVEEEEEVAAPREKEKSDKVAAGDEEVEGTANALEEVPVAQRGLAGRAPEGMFSTECAASNNGGKEINEGGGKEENGKMEAVNSRGELEDDEKGEEHPGRSSLDTITLREWFERMERCLPRMINEAADEMIAKLEEKHRRINEYISVLGNSSDPSFDPCIVRSYAQSNAG</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
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                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="112"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="379"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="432" mass="47386" checksum="4DAB9928B37A8092" modified="2022-05-25" version="1">MNGVSEGLIIGTTVGVVIGVLLAVGILLCMRYRRSQAQIRSSSSRRSSTIPMRANGVNTCAELSNSTTGQESPREFEDRGPSLWIEGPGRKSLISASGIPKYAYKELQKATSNFTKLLGQGAFGPVYKADMSSGEILAVKVLANNSKQGEKEFQNEVLLLGRLHHRNLVNLVGYCAEKGQHILLYAYMPNGSLASHLYGENSAPLRWELRVNIALDVARGLEYLHDGAVPPVVHRDIKSPNILLDQSMHARVADFGLSREEMLTRNGANIRGTFGYLDPEYVSSRSFTKKSDVYSYGVLLFELIAGRNPQQGLMEYVELAAINADGKTGWEEIADSRLEGAFDVEELNDMAAAAYKCVSRVSRKRPSMRDVVQALIRVAKHSHSSRNHHGRRLPPGRTDDESVDLEASEDQSSASGHQRQESVGSVSELPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B211</accession>
    <name>A0A8I7B211_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123429249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044969240.1">
        <property type="nucleotide sequence ID" value="XM_045113305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B211"/>
    <dbReference type="SMR" id="A0A8I7B211"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0104270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0104270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0104270"/>
    </dbReference>
    <dbReference type="GeneID" id="123429249"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0085640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0085640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0085640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0104270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0104270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0104270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123429249"/>
    <dbReference type="OMA" id="DVCQAKA"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08295">
        <property type="entry name" value="double_bond_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF60">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="25"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0104270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="346" mass="37648" checksum="F768677AF7210277" modified="2022-05-25" version="1">MAAAAEVSNRSVILKRHVTGFPTEDDMEVVTAAARVAVPPGSAAVMVKNLYLSCDPYMRSRMTRHLEPSYVPDYVPGEVIPTFGVCKVVESGHPDYKAGDMVWGMTACEEYTLITNPESLFKINHPELPLSYYAGVLGMPGLTAYAGFFDVAKPNKGDYVFVSAASGAVGQLVGQLAKITGCYVVGSAGSDEKVNLLKTKFGFDDAFNYKKEHDLNATLKRCFPEGIDIYFDNVGGAMLDAALLNMRLHGRVAMCGLISQYNLEQPEGVRNLACVVTKRVRMEGFLVTDYFGTYRKFEEETGGHLKEGNITYVEDVAEGIEKVATALVGLFHGHNIGKQLVAVAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4B4</accession>
    <name>A0A8I7B4B4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4B4"/>
    <dbReference type="SMR" id="A0A8I7B4B4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213460.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="209"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213460.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="619" mass="72201" checksum="BD5116765AE75EB5" modified="2022-05-25" version="1">MSGIKKIHNQLAYNIEAYINDTKYIIDEITIRYLSVINDDMIIGLRFLQQSLQTTIIHEEGVTFIPYQDNLPYISEVRKQRKALQNVEISNLDGEYSTSPEVALEFCKDEELSETIEEYHIENTSTECIALQSFSPNWYRDIKSKKDIDKIVQRLENIQIIGEIPMKHWERNSIVCKINIINPDYIIKSGPIEATPKDIEEFKMHIEELLKLKAIRESRSPHRSAAFIVRNHAEEVRGKSRMVINFKRLNDNTVDDAYNIPNKQEWINRIQGSKFFSKFDLKAGFWQVKMAEESIPWTAFTCPQGHYEWLVMPLGLKNAPPLFQRKMQNIFNENQAFILVYVDDLLVFSKSYKEHIAHLEVFFRKVEQNGLILSKKKMEICKERINFLGHEIGEGKIYLQEHIAKKILEFPDNMNDKKVLQQFLGIVNYARNYIDNLAKLAGPLYAKLRKNGQRYFNSEDIKLVKAIKEKVKNLKPLELPLEDNYFIIETDASKVGWGAILKQKPNKYSPKADEKICRYASGSYKLKTVNNIDREILAVVNAINAFRLYLGFKEFTVRTDCEAICRYYNKINSKKSSTRRWVLFEDTIAGNGYKVIFEHIKGKDNTLPDIFSRASNLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5P2</accession>
    <name>A0A8I7B5P2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123444268</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976880.1">
        <property type="nucleotide sequence ID" value="XM_045120945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5P2"/>
    <dbReference type="SMR" id="A0A8I7B5P2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284880"/>
    </dbReference>
    <dbReference type="GeneID" id="123444268"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0236310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0236310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0236310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0284880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0284880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0284880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444268"/>
    <dbReference type="OMA" id="CNALMTM"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000125">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000333">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000767">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000842">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF436">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="139"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="201"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0284880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="61966" checksum="282E867962233911" modified="2022-05-25" version="1">MHRARAPAIVASRLLVRDNQRITALARAGDLAAARRVFDAMPRRDVVSWNALLTALWRVGRDLPAARRLFDDMPSRNVISWNSVVAGCLAHGDLAAASAYFARTPRRNVASWNAMLAGLVRLGCMNDAERLFGEMPERNVVSYTTMVDGLARRGEVGRARAVFDEMPERNLVSWAAMISGYVDNSMLDEARKLFAAMPEKNVMACTAMITGYCKGGDVESARKLFDEIYVKDVISWNAMITGYVHNGYGEEALKLHIIMLREGTKPDHATLIAILTACSALALLRQGRSTHAVAVKTMLESSTSFCNALITMYSKCGDVTESELVFINLKSQDIVSWNTMIAAYAQHGKYQKAISLFHEMETRELIPNDITILSVLSACGHVGRVDDSLELFDLMSSKYAICPGAEHYACIIDILGRAGQFEKACSYIKDMPFEAEKNVWGALLGASRTHGNVQLGELAAKMLVQSHSGSSGPYVMLSNIYAAAGMWGQVNRIRGQMKENGVKKQPGYSWTEIANRVHMFVGGDASHPEMNKIISELRKISFHMQMMANGTHTMVEMSQESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7C0</accession>
    <name>A0A8I7B7C0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123427268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967204.1">
        <property type="nucleotide sequence ID" value="XM_045111269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7C0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0182430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0182430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0182430"/>
    </dbReference>
    <dbReference type="GeneID" id="123427268"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0150930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0150930.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0150930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0182430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0182430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0182430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427268"/>
    <dbReference type="OrthoDB" id="672370at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37697">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37697:SF2">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0182430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="151" mass="15465" checksum="83A5C5BBC62EB190" modified="2022-05-25" version="1">MELDLPPPRQAPPLTDLAAAIRLAAEAAAALSAPSSSSAAAAAAATLRDAHAAIGSFLSRLDAPAAAPFDSDQPMADGGEAGDPMIGEVEDGLRECALQGNKRRKRPVPPSWPLGPRTSGACVAPEATAEPVLDVQRRRGAAMDLLLQFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAF3</accession>
    <name>A0A8I7BAF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase RNF170</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RING finger protein 170</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RING-type E3 ubiquitin transferase RNF170</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123394969</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044945811.1">
        <property type="nucleotide sequence ID" value="XM_045089876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530970"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
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            <begin position="20"/>
            <end position="63"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="195" mass="22069" checksum="B617185FA929B9F4" modified="2022-05-25" version="1">MSSAAVSSSASPVPPEDDVCSVCHDRFRIPCQANCSHWFCGECIIRVWNHGAAVQPCKCPICRRLINLLVPANVSDDQNDDPQLQRVLGEIQHYNHIFGGAPRSLTQRLQDLPFFIRRLFRELMDPQRTLPLVFRARMILMVILSGVYVLSPVDILPESVLGLFGFFDDFLILVIVFLHLAAVYRSLLLYRHGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAZ2</accession>
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        </recommendedName>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB88</accession>
    <name>A0A8I7BB88_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB88"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0557460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0557460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0557460"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0557460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="315" mass="35205" checksum="78BE4EB910CA6A0F" modified="2022-05-25" version="1">MKRKLYILFFTLEGFDQIGEETDGDDVDPDEDNHEVNDEEQGGEEEGDDMGEDGEDEAIDEIDKANFQSKNLENGSDNKCVQVGDVCAANFHPHILLDEMHENFVEETREKLPQEGMLDLETDMGKELDWDMGSPICTSDVPMSVHLDYCSEQLRKIDMMESNEEEEDVMPEMQCLAKEICTVLGSTKRSLLDTLEKEAGQKKKINAGKDGRWGPVLSNKPWTREHGGGVKIMDKAAAYMQKKNLEIPANFKGPILEKCKMHSCKAGHLSKSMEDIVHRCSANSSAGLHQIVEQDTWGIASDCVEMMLGGHMGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDB6</accession>
    <name>A0A8I7BDB6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123408193</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957295.1">
        <property type="nucleotide sequence ID" value="XM_045101360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDB6"/>
    <dbReference type="SMR" id="A0A8I7BDB6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653660"/>
    </dbReference>
    <dbReference type="GeneID" id="123408193"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408193"/>
    <dbReference type="OrthoDB" id="637156at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF23">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="108" mass="12193" checksum="760BDF091CAB450E" modified="2022-05-25" version="1">MAKRKSMSSKMASRKKPAPKLETTFCCPFCNHPDGVACTIDLKLFVASAVCYVCQEAYHTAAHHLTEPVDIYHEWIDACEKANQDVDDVDCYKRQRRVGSGDDDDRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEK2</accession>
    <name>A0A8I7BEK2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase (cysteine targeting)</fullName>
            <ecNumber evidence="17">2.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123410613</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.36" evidence="17"/>
    <dbReference type="RefSeq" id="XP_044959491.1">
        <property type="nucleotide sequence ID" value="XM_045103556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEK2"/>
    <dbReference type="SMR" id="A0A8I7BEK2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0700750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0700750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0700750"/>
    </dbReference>
    <dbReference type="GeneID" id="123410613"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0700750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0700750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0700750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410613"/>
    <dbReference type="OrthoDB" id="1701437at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd16526">
        <property type="entry name" value="RING-HC_PEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045859">
        <property type="entry name" value="RING-HC_PEX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178:SF1">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0700750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="298" mass="33873" checksum="95BD5D41E3D0E7A4" modified="2022-05-25" version="1">MIPITISRVNQFDAARLDVEMSAMLKEQLVKVFSLMKPGLLFQYEPELDAFLEFLIWRFSIWVDKPTPGNALMNLRYRDERAAPIAGKEVRTGLEGPGLSVSQKILYCISTVGGQYLLSRLQSFSAFRRWGDSEQRPLARRAWGLVQHAEGLYRAVSFFNLLSFLYGGRYKTLVERILKARLVYGSPNMNRAVSFEYMNRQLVWNEFSEMLLLLLPLLNSSSVKKFLLPFSKDKSASSSGDEANCPICVSSPSIPFVALPCQHRYCYYCLRTRSAATSSYRCARCNEVVVAIQRHGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEV2</accession>
    <name>A0A8I7BEV2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEV2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0591620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0591620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0591620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0713230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47719">
        <property type="entry name" value="SKP1-INTERACTING PARTNER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47719:SF2">
        <property type="entry name" value="SKP1-INTERACTING PARTNER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="16419" checksum="4DB1C17568E9A6CE" modified="2022-05-25" version="1">MIGGLRSSFALDAPCSTVLILRLDLATMEWDEASRMPPNMYRCFTGLCEAAAQGSAMPPAPVAGGNNKVKVFGGDGKVWFGGKRVRGKLAMWEEDDVGSSGGKWDWVDGVPGYGDGVYRGFVCGGKWDWVDGVPGYGDGVYRGFVFDGGFTAMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJV7</accession>
    <name>A0A8I7BJV7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 8">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123411871</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 8"/>
    <dbReference type="RefSeq" id="XP_044960764.1">
        <property type="nucleotide sequence ID" value="XM_045104829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJV7"/>
    <dbReference type="SMR" id="A0A8I7BJV7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0733480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0733480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0733480"/>
    </dbReference>
    <dbReference type="GeneID" id="123411871"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0608730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0608730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0608730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0733480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0733480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0733480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411871"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00884">
        <property type="entry name" value="beta_CA_cladeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1050.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045066">
        <property type="entry name" value="Beta_CA_cladeB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF80">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0733480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="201" mass="22347" checksum="051CBC51E2C2070D" modified="2022-05-25" version="1">MDPVERLRTGFEKFKTEVYDQKPDLFEPLKAHQSPKYMVFACADSRVCPSVTLGLEPGEAFMVRNIAAMVPCYCKNKHTGVGSAIEYAVCALKVEVIVVIGHSRCGGIKALLNLKDGADDTFHFVEDWVRIGFPARKKVKDECSEMPFEDQCGVLEKEAVNVSLQNLSTYPFVKEGVANGTLKLVGGHYDFVSGKFDTWEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2CPR9</accession>
    <name>F2CPR9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123427217</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
            <tissue evidence="10">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK353621">
        <property type="protein sequence ID" value="BAJ84840.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK354234">
        <property type="protein sequence ID" value="BAJ85453.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967148.1">
        <property type="nucleotide sequence ID" value="XM_045111213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CPR9"/>
    <dbReference type="SMR" id="F2CPR9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180820"/>
    </dbReference>
    <dbReference type="GeneID" id="123427217"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0149500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0149500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0149500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427217"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000006">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF121">
        <property type="entry name" value="AMMONIUM TRANSPORTER 1 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="39"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ84840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ85453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="494" mass="52315" checksum="9DC19AE3876096C1" modified="2011-05-31" version="1">MSATCAADLGPLLGAAAANATDYLCNRFADTTSAVDSTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGTPSNGFIGKHFFGLKDMPQTGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVYPVVSHWIWSVDGWASAARTSGPLLFKSGVIDFAGSGVVHMVGGIAGFWGALIEGPRIGRFDHAGRSVALKGHSASLVVLGTFLLWFGWYGFNPGSFVTILKSYGPPGSIHGQWSGVGRTAVTTTLAGSVAALTTLFGKRLQTGHWNVVDVCNGLLGGFAAITAGCSVVDPWAAVICGFVSAWVLIGLNALAARLKYDDPLEAAQLHGGCGAWGIIFTALFAKRQYVEEIYGPGRPYGLFLGGGGRLLAAHIVQILVIAGFVSCTMGPLFFALKKLGLLRISAEDEMAGMDLTRHGGFAYVYHDDDEHDKSVGGFMLRSAQTRVEPAAAANSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F2CPS5</accession>
    <name>F2CPS5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123395122</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the nascent polypeptide-associated complex (NAC).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NAC-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AK353627">
        <property type="protein sequence ID" value="BAJ84846.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945993.1">
        <property type="nucleotide sequence ID" value="XM_045090058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CPS5"/>
    <dbReference type="SMR" id="F2CPS5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534510"/>
    </dbReference>
    <dbReference type="GeneID" id="123395122"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0444660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0444660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0444660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0534510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0534510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0534510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395122"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000001">
        <property type="entry name" value="Transcription factor BTF3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC-A/B" evidence="4">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ84846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0534510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="157" mass="17312" checksum="D57DEAD5EBFF059A" modified="2011-05-31" version="1">MNKERLMKMAGAVRTGGKGTVRRKKKAVHKTATTDDKRLQSTLKRVGVNTIPAIEEVNIFKDDLVIQFLNPKVQASIAANTWVVSGTPQTKKLQDVLPSIINQLGPDNMEHLKRIAEEMQKQVAAAGAIQPKEENDDDVPELVPGETFEEVAQETKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F2CR07</accession>
    <name>F2CR07_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123406391</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
            <tissue evidence="3">Shoot</tissue>
            <tissue evidence="5">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK354059">
        <property type="protein sequence ID" value="BAJ85278.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK358979">
        <property type="protein sequence ID" value="BAJ90190.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK360834">
        <property type="protein sequence ID" value="BAJ92042.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK365537">
        <property type="protein sequence ID" value="BAJ96740.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955830.1">
        <property type="nucleotide sequence ID" value="XM_045099895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CR07"/>
    <dbReference type="STRING" id="112509.F2CR07"/>
    <dbReference type="PaxDb" id="4513-MLOC_15214.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612480"/>
    </dbReference>
    <dbReference type="GeneID" id="123406391"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0507860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0507860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0507860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0612480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0612480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0612480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406391"/>
    <dbReference type="eggNOG" id="ENOG502RRD0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053941_1_0_1"/>
    <dbReference type="OMA" id="GCSVGMS"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CR07">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF80">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="195"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ85278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ90190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ96740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0612480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="40146" checksum="88640143344E5503" modified="2011-05-31" version="1">MAAILGRAGTTATGCFRRHHEETRRRGRRRNAVVVAAVTGTAPQEGSLERPAWSGETPVSRLVAALIAIKPLYSLMKLASREVIIRTAEKSNIPWREMTKKVLESDVYEVFERIRDPNIVYPDYYLSPFHAYDEGNLSWLAAAEAEAATLSIAKRAIPEATTIEEANRIVRGNWMNAIEEHHLKYSGGNCQINDILDIGCSVGVSTRYLAEKFPSAKAVGLDLSPYFLSVAAQKEEQLSRQNPIRWVHANGEATGLPPNSFDVVSLAYVCHECPARAIIGLAKEAFRLLRPGGTIALTDNSPKSKVLQELSPVLFTLMKSTEPFLDEYYMLDLEDALSQAGFVNVCSVLTDPRHRTVTATVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2CV07</accession>
    <name>F2CV07_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123450057</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
            <location evidence="5">Extracellular space</location>
            <location evidence="5">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK355459">
        <property type="protein sequence ID" value="BAJ86678.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983389.1">
        <property type="nucleotide sequence ID" value="XM_045127454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CV07"/>
    <dbReference type="SMR" id="F2CV07"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0418330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0418330"/>
    </dbReference>
    <dbReference type="GeneID" id="123450057"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0348720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0348720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0348720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0418330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0418330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0418330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450057"/>
    <dbReference type="OrthoDB" id="590363at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09612">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033734">
        <property type="entry name" value="Jacalin-like_lectin_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="6">
        <location>
            <begin position="234"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ86678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="392" mass="42213" checksum="E2DEED9B4FE5A774" modified="2011-05-31" version="1">MAEPSYLQSVTLSQESQLKEVVLHLYLYQNSQAQPKGNQCQIVGSTRPHAFGATVVNDWTIHDGPSPTANIVARAQGLHLQAGMTAGNWFVCHNIVFTDDRFKGSTLNVLGNIEGQEGEWAIVGGTGEFAYAQGVVSYKPITSTTQGKVTRELHIRALCITLLKPPAPVVPTASTVAASSEMVAQAVVPPPAVPAPPVLPTRSEVVPTPAVPALPVLPTRSEVVPTPVDFPMVLTKYGPWGPENGKYKDMQNELPQRLVRVTFFHGLYIYIMSFSYIDHSGVTKSCQLGGRPVSGSMPKEMNLEDGEFVKSVSGTTGLFDGDARLTSVSFVTNLRSYGPFGREGKHASFSTPELNNDSSIVGFFGWHVSNFAIDSTNYLSSLGVYVASPRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2D0R5</accession>
    <name>F2D0R5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123431057</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357472">
        <property type="protein sequence ID" value="BAJ88686.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970805.1">
        <property type="nucleotide sequence ID" value="XM_045114870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D0R5"/>
    <dbReference type="SMR" id="F2D0R5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0205420"/>
    </dbReference>
    <dbReference type="GeneID" id="123431057"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0170900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0170900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0170900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0205420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0205420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0205420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431057"/>
    <dbReference type="HOGENOM" id="CLU_060839_3_1_1"/>
    <dbReference type="OrthoDB" id="785270at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044169">
        <property type="entry name" value="PI21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47488">
        <property type="entry name" value="HEAVY METAL TRANSPORT/DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47488:SF21">
        <property type="entry name" value="HMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ88686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0205420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="335" mass="37204" checksum="3C94D1A0E59522B1" modified="2011-05-31" version="1">MSDQISTIILRVDLDCHLCYKKIRKILCNLQDQERIRTISFDTNNNAVIIDGPFDPHKLSCKIRCKGGKVIKGVQIMGDGKPEQMAGPPPSNSRKKKSKSKGKGKESPPPPAEQLPPAYHPQPPTEQPQTYYQPPPPTEQPPAYHQQPPTEQPPEYHPQQPTEQQPVYHPQPPAEQPPEYHPQPPPPDREMSATMPAVREEERPKERPAELEAPKEMQVHFVPEAGPECHKNPRERRHPMGEMGPWPGPPMVEIPSLPARPVGPCRCMCCASCYQGYYASCRCSDCGRTYGYTAAVALPPPAGCYGGARTPYCGGYSGGYRTICEEDQPAACVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2D1J2</accession>
    <name>F2D1J2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cysteine synthase</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123408554</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="AK357749">
        <property type="protein sequence ID" value="BAJ88963.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957570.1">
        <property type="nucleotide sequence ID" value="XM_045101635.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957571.1">
        <property type="nucleotide sequence ID" value="XM_045101636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D1J2"/>
    <dbReference type="SMR" id="F2D1J2"/>
    <dbReference type="PaxDb" id="4513-MLOC_65981.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717380.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717380.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717380"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717380.4">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717380.4"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717380"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717380.5">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717380.5"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717380"/>
    </dbReference>
    <dbReference type="GeneID" id="123408554"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0595080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0595080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0595080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717380.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717380.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717380.4">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717380.4"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717380.5">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717380.5"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408554"/>
    <dbReference type="eggNOG" id="KOG1252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_1"/>
    <dbReference type="OMA" id="KCILVMP"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000067">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="12">
        <location>
            <begin position="26"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ88963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717380.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="36395" checksum="25CD72F1A7569A86" modified="2011-05-31" version="1">MAVEEEGRKGIPSLLSSEGENIASNITQLIGWTPLIEMKKIAKKDGVEARLIGKMEAYQPLCSVKDRAALRMIEDAEEKGLISPGVTTLIEPTGGNQGIGMVFIAVQKGYRFIAVMPAKYSLDKQMLLRFLGAELVLTDPANGFKGMIGKVEELMKTTPNSHCLNQVTNPANPDAHFRWTGPEIWKDTAGKVDMFVAAVGTGGTLTGVGKYLKMKNPSIKIVCVEPSESAVISGGSPGSHKIQGTGPGFIPEVLDTSVIDEVITVSTEEAMAMARRLAREEGLLVGISSGANVAACVKIAAREENQGKMIVTIFPSAGERYMNSDLFALVREECDNMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2C3</accession>
    <name>F2D2C3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123411935</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
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        <property type="molecule type" value="mRNA"/>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
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                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
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            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="18">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
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                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
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    <reference evidence="19" key="3">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <property type="molecule type" value="mRNA"/>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412300.1">
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <location>
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            <end position="25"/>
        </location>
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            <end position="873"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
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    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
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            <end position="802"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="453"/>
            <end position="495"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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            <begin position="465"/>
            <end position="476"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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            <end position="495"/>
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    <feature type="binding site" evidence="12">
        <location>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BAJ93288.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="873" mass="94204" checksum="74A34106AF72E0CE" modified="2011-05-31" version="1">MAVHVLLPLLLLLLVPTVLPYIALAASSPDFTIFLACGAGANISFPSDNPARTFVPDAGYFSPARAPAVSAGASSSAASPLYAAARAGSSDFSYRLTYPDTAGASSFLVLRLHFFPFVPASSSTSLSSARFTVSVLDAYALLRNFSPPADGIVKEFFLPRGRSGDFTVRFSPDAGSSAFVNAVELFPAPPELLWNGSTSVVPVGVLEGTGLPQWQLAALETVYRLNVGGPMVTRENDTLWRTWLPDGPYLFGAPGQSVVNNTSSPIIYNPPTTREVAPDVVYRTQRAANVTDLMRATTPGLNFNVTWTFPAEAGSRYLVRLHFCDYEVVSSVVGVGIVFNVYVAQALATLDLSPEDRARQPNEAFYVDYAAMAPRAGNLTVSIGWSPKSSGGGILNGLEIMKLQSANLSSPRPHGLTKKTIIVIVLATVLGAAVLACAVLCFFVVLRRKRRQVAPPASTEDKESTQLPWSPYTQEGVSGWADESTNRSSEGTTARMQRVSTKLHISLAEVKAATDNFHDRNLIGVGGFGNVYKGALADGTPVAVKRAMRASKQGLPEFQTEIVVLSGIRHRHLVALIGYCNEQAEMILVYEYMEKGTLRSHLYGSDEPTLSWKQRLEICIGAARGLHYLHCGYSENIIHRDVKSTNILLGTDDHGGGSASGGAAIIAKVADFGLSRIGPSLGETHVSTAVKGSFGYLDPEYFKTQQLTDRSDVYSFGVVLFEVLCARPVIDQSLDRDQINIAEWAVRMHGEGKLDKIADARIAGEVNENSLRKFAETAEKCLAEYGADRPSMGDVLWNLEYCLQLQETHVNRDAFEDSGAVATQLPADVVVPRWVPSSTSMLMMDDADETGLSMTEIADSQVFSQLNARGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7E2</accession>
    <name>F2D7E2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123443838</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AK359804">
        <property type="protein sequence ID" value="BAJ91013.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976300.1">
        <property type="nucleotide sequence ID" value="XM_045120365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D7E2"/>
    <dbReference type="SMR" id="F2D7E2"/>
    <dbReference type="STRING" id="112509.F2D7E2"/>
    <dbReference type="PaxDb" id="4513-MLOC_57405.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0269960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0269960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0269960"/>
    </dbReference>
    <dbReference type="GeneID" id="123443838"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0223730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0223730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0223730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0269960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0269960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0269960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443838"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D7E2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF1">
        <property type="entry name" value="OS01G0601200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="188"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAJ91013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0269960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="483" mass="54258" checksum="7CE56163D0117F4C" modified="2011-05-31" version="1">MSTSENLRVELLSRTPPFGLRLWVVLGISIWAAILFVLGLICFLLIYWRRRGNHHDIAEPEIPDVTKEIAVDEVGSRAFVDNICAPESHIFPVKERDPEKESQRMSAHLITSKSIDDHNLIECSSVHQYDRSQGLYSGDEGSSAYDKREYSQCGTMSMSPRGGLPEFSHLGLGHWYTLRELEHSTNGFSNEYIIGEGGYGVVYHGCLVNGTDVAIKKLFNNVGQAEKEFRVEVEAIGHVRHKNLVRLLGYCVEGSHRMLVYEYISNGNLEQWLHGAMRQQGVLTWEARIKITLGIAKALAYLHEGIEPKVIHRDIKSSNILIDEEFNGKLSDFGLSKLLGEGKSHITTRVMGTFGYVAPEYVNTGLLNEKSDVYSFGVLLLEAVTGRDPVNYSRPAKEVHMVEWLKLMVGSRRAEEVVDPEMEAKPTKQALKRALLVALKCVDPVADRRPTMGQAVRMLEAEDVLSREERRKSRLAHVDGGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F2DMD8</accession>
    <name>F2DMD8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">12-oxophytodienoic acid reductase 2</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">OPR2</name>
        <name evidence="15" type="synonym">LOC123426804</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2013-02" db="EMBL/GenBank/DDBJ databases">
            <title>Isolation and Expression Analysis of Putative Oxo-Phytodienoic Acid Reductase Gene from Barley.</title>
            <authorList>
                <person name="Al-Momany B."/>
                <person name="Abu-Romman S."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KC661078">
        <property type="protein sequence ID" value="AGG17917.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK365056">
        <property type="protein sequence ID" value="BAJ96259.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DMD8"/>
    <dbReference type="SMR" id="F2DMD8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0170580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0170580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0170580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0140860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0140860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0140860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0170580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0170580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0170580"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_0_1"/>
    <dbReference type="OrthoDB" id="1663137at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DMD8">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000073">
        <property type="entry name" value="12-oxophytodienoate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF119">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGG17917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ96259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0170580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="366" mass="40522" checksum="6BEF238F92F24C03" modified="2011-05-31" version="1">MSNTALLTPYKMGRFDLSHRVVLAPLTRERSFGNVPQPHAVLYYQQRTTEGGLLIAEATGVSDTAQGYKDTPGIWTKEQVEAWKPIVNGVHAKGGIFFCQIWHVGRVSNHTFQPNGQAPISSTDKPLKPVVRANGIDVATISTPRRLQTNEIPLVINDFRVAARNAIEAGFDGVEIHGAHGYLIDQFLKDQVNDRTDKYGGSLENRCRFALEVVQAVVDEVGADKVGIRLSPFASYSEASDSNPEALGLYMAHALNKFGILYCHMVEPRMVNIGEKFETPHSLRPIRDAFKGTFIVAGGYDRDDGNKAIADGYADLVAYGRLFLSNPDLPRRFEIGAPLNKYIRDTFYLSDPVIGYTDYPFLPSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2DTZ3</accession>
    <name>F2DTZ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440550</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367361">
        <property type="protein sequence ID" value="BAJ98564.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973050.1">
        <property type="nucleotide sequence ID" value="XM_045117115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DTZ3"/>
    <dbReference type="SMR" id="F2DTZ3"/>
    <dbReference type="PaxDb" id="4513-MLOC_44247.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303210"/>
    </dbReference>
    <dbReference type="GeneID" id="123440550"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0303210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440550"/>
    <dbReference type="HOGENOM" id="CLU_026209_0_1_1"/>
    <dbReference type="OMA" id="DMIDKVC"/>
    <dbReference type="OrthoDB" id="2498029at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DTZ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000036">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="87"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ98564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="352" mass="39244" checksum="CA8E6D2F253BFA5B" modified="2011-05-31" version="1">MNLSPGRKVRVSVHISSTTPRFPSSPVACCYLQRSLGDSDRLTYPQGISKTSPMAHYKDDIKYEEGFTLNSRGSRLFTCKWTPKKQQRKALIFICHGYAGECSISMADTAARLVHAGYAVHGIDQEGHGKSSGSKGYISSFSDIVKDCSDYFKSVCEKPENKTKKRFLYGFSMGGTVALQVHRKDSMYWDGAVLLAPMVKLGDGMRPHPVVVSALKMICAVVPSWRVIPAPDQLDKVCKDPQFKKEIRSNPYMYKGNIALQTGHELLAVSLDIEKNMHEVTLPFLVLQGEDDVVADPEGSRLLHERASSRDKTLKLYPGMWHVLMAEPPADVERIFVDVISWLNERAVSASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2DUT8</accession>
    <name>F2DUT8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123413603</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK367656">
        <property type="protein sequence ID" value="BAJ98859.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962474.1">
        <property type="nucleotide sequence ID" value="XM_045106539.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962475.1">
        <property type="nucleotide sequence ID" value="XM_045106540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DUT8"/>
    <dbReference type="SMR" id="F2DUT8"/>
    <dbReference type="STRING" id="112509.F2DUT8"/>
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    <dbReference type="GeneID" id="123413603"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0528910.1.CDS.1"/>
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        <property type="entry name" value="OS02G0104200 PROTEIN"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ98859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636530.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="25954" checksum="AFF99ABF5F2856FF" modified="2011-05-31" version="1">MDRAGGNQAGQVSKKGKKKHAKDESDRQKQAEKKRRRLEKALANSAAIISELEKKRQQKQEEQRRLDDEGAAIAEAVALHVLIGEDCDEPRQLMWNGHRGRGHRDDFVDHERAPGAQRAGGDAYPCGSRSPLARASRSHMPQWRLTDCGMSGPFSFSSWERMGDFEGLYCEGTPCQTDQDTYCHGGLVAATQAVSPFGYVSEDPFPAHGTEGASSINIMLGGTCSNSLNIYRRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F2E067</accession>
    <name>F2E067_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123403105</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="AK369538">
        <property type="protein sequence ID" value="BAK00739.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044953007.1">
        <property type="nucleotide sequence ID" value="XM_045097072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E067"/>
    <dbReference type="SMR" id="F2E067"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558870"/>
    </dbReference>
    <dbReference type="GeneID" id="123403105"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0464330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0464330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0464330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0558870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558870.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403105"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK00739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558870.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="155" mass="16280" checksum="FB715DAE4BDBBF84" modified="2011-05-31" version="1">MDASATVAAGKGKKGAAGRKAGGPRKKSVSRSVKAGLQFPVSRIGRFLKKGRYAQRVGSGAPVYLAAVLEYLAAELLELAGNAAKDNKKSRITPRHLLLAIRNDEELGKLLAGITIAHGGVIPNINPVLLPKKTAEKSPKEPKSPKKTAKSPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2E1C6</accession>
    <name>F2E1C6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123411959</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHF5 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAK01148.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960854.1">
        <property type="nucleotide sequence ID" value="XM_045104919.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960855.1">
        <property type="nucleotide sequence ID" value="XM_045104920.1"/>
    </dbReference>
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    <dbReference type="STRING" id="112509.F2E1C6"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0201110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0201110.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0201110"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0720690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0720690"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720690.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0720690.2.CDS2"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.2HG0167090"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0597670.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0597670"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0201110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0201110.1.CDS1"/>
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    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0720690"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110369_2_0_1"/>
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    <dbReference type="OrthoDB" id="1928519at2759"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="PHF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13120">
        <property type="entry name" value="PHD FINGER-LIKE DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03660">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016468">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK01148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0201110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="110" mass="12415" checksum="548B04E147361E28" modified="2011-05-31" version="1">MAKHHPDLIMCRKQPGIAIGRLCEKCDGKCVICDSYVRPCTLVRVCDECNYGSFQGRCVICGGVGISDAYYCKECTQQEKDRDGCPKIVNLGSAKTDLFYERKKYGFKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2E2F9</accession>
    <name>F2E2F9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123399470</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK370330">
        <property type="protein sequence ID" value="BAK01531.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949817.1">
        <property type="nucleotide sequence ID" value="XM_045093882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E2F9"/>
    <dbReference type="SMR" id="F2E2F9"/>
    <dbReference type="PaxDb" id="4513-MLOC_73322.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491250"/>
    </dbReference>
    <dbReference type="GeneID" id="123399470"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0407160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0407160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0407160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0491250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0491250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0491250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399470"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E2F9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF840">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK01531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0491250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="342" mass="37914" checksum="5D5FEA32163FDBB8" modified="2011-05-31" version="1">MEPPRHVCVTGGGGFIASWLVKLLLSRGYAVHATVRDPSDQKNAHLMQLDGAAENLRLFKADVLDSAALAAAVEGCEGVFHVASPVPADKILDPESEVMVPAVKGTLNTLEVCSSLKVQKVVVVSSTAAVRYNPNWPLGKPKDESCWSDRKVCKDNEIWYCLAKTVAEQTVWEYAEKNELNAVTVCPCIVLGPQLQRVVNTTSELLLYVIKGGPNVLNDMLWHIVDVRDVADALLLVYEKPESSGRYICAPNYISTNAMLELLKKTYPDYNYVKCKADVHQNSPTTLITSGKLNDLGWKPRALEETLLDSIDYYRKTGILQDGEGQTYRLPDIFRRFQAAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F2E8F3</accession>
    <name>F2E8F3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123412787</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Flower</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK372427">
        <property type="protein sequence ID" value="BAK03625.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961655.1">
        <property type="nucleotide sequence ID" value="XM_045105720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E8F3"/>
    <dbReference type="SMR" id="F2E8F3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0716390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0716390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0716390"/>
    </dbReference>
    <dbReference type="GeneID" id="123412787"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0594270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0594270.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0594270"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0716390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0716390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0716390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412787"/>
    <dbReference type="HOGENOM" id="CLU_043885_0_0_1"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010705">
        <property type="term" value="P:meiotic DNA double-strand break processing involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039685">
        <property type="entry name" value="FANCE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32094">
        <property type="entry name" value="FANCONI ANEMIA GROUP E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32094:SF5">
        <property type="entry name" value="FANCONI ANEMIA GROUP E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAK03625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0716390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="477" mass="51993" checksum="FF3ACEB65F88DCC6" modified="2011-05-31" version="1">MEPWLPVLRHLLATSAPSAAAFSSSNSSPCSLPPAAGLLRLLLSPVPTLPASEPTPVLFQTLPPLLQSQALSFLSSSASLLDPLQLRSLASRVLSAPPGRCDEFWVRRGAHHLLDGLPDKDVPHVPWEFNDEFHEPPPWLKGEAARTQPALPWLPLDCRSMMPGGVHASEDGLSEVRLESLGLEQDGCPLMQEVGCAPSPPASPLENSVVHRALALKKEIGMAESVLGAQQAANNLHDLCLESGNAEAVLSLVQPWEADDDIVRVLLSNLVLEEDGMHGKGPALVLCSLALPKLLDLQRAASSGLLSAALDICKSYPAAALEAVLFPLVLRKQGLNAPQCDVLTRIVKDCMHPLHVTAFCHRLLSGEEGERKPVCMPQHRENIGRDLVWTESLFALFYNILSQDICLTPSTIEKLVSMIHDMAIKFPKSLKFGNLFLCFVSKCWHECKIHRVLLERAAETTNTFLTKAILVKLRPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECS7</accession>
    <name>F2ECS7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123448633</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Flower</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK373952">
        <property type="protein sequence ID" value="BAK05149.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECS7"/>
    <dbReference type="SMR" id="F2ECS7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0375930.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0375930.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0375930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0312900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0312900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0312900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0375930.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0375930.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0375930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090310">
        <property type="term" value="P:negative regulation of DNA methylation-dependent heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070920">
        <property type="term" value="P:regulation of regulatory ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF17">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE JMJ24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08879">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51667">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WRC" evidence="12">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="141"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="579"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="679"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK05149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0375930.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="890" mass="99544" checksum="B8036D65B7A614C9" modified="2011-05-31" version="1">MDAVPEDLRCKRSDGKQWRCSAPSMPDKTVCEKHYVQAKKRAASSALRATLRRSSPSYAAASYPPPHEGDSDADADLPLHLPMAVARPFYGRLVGEPVYVAEPAVRRGPPLNNAIGSRTAAELVGRPYVGLQGCAEVKTSCHQCRRSVNAVWCTSCDRRGYCDGCISRWYSDIAIDDIQKVCPACRGICNCKVCLQGDNLIKARVQEISVVDKLKYLHSILAYVLPVLKQIYSDQCFEIGVETRACGPKMDIIRAKMNSDEQMCCDFCKVPVFDYHRHCPRCLYDLCLDCCRDIRRSQTNVVRGEYAESKGHVVERNKDASNRARSEPSAASVDDKLFSQPIDANDIGIRSLFTTWRVNNDGSITCGPRGAGGCGSSKLVLRRIFKINWIGKLVKSSQEMVNGCKAHDLENGCSSCNASRRLDSIGRRNFGLSNCSASDGTDGNYVYSSVLENLKYEGIVHFRKHWINGEPVVIRNAFEPSLSSSWDPLSIWRGIQEIMDEKMDENAIVKAVDCSNQSEVHIKLNQFIKGYSDGHKGEDGKLMMLKLKEWPPVSVLEEFLLCQRPEFIVNFPLVDFIHSKWGFLNLAAKLPPDALQSEVSLKLLIAYGRQQETGNNDSVTNLMVKMGDVVHMLMHTAEMPDLCRKSPQPEQPEMIANGMTVHVNAHAPVQNLNLDMGEQSPEHTVSKSCGDSVGSCPEQPKSNGLERSQPGALWDVFRRQDVPMLNKYLASNWEELTVSSQAMLSVKHPIYDQAVYLKEHHKRVLKDQYGIEPRTFEQHIGEAVFIPAGCPFQVKNLQSTVQLALDFLLPESLWESARMGQEIRCLPNHHDAKLKMLEVGKISLYAASSAVKEIQKITLDPKFNLDIRFEDQNLTRAVSENLARVTKQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2EHK4</accession>
    <name>F2EHK4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123401784</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375631">
        <property type="protein sequence ID" value="BAK06826.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951570.1">
        <property type="nucleotide sequence ID" value="XM_045095635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EHK4"/>
    <dbReference type="SMR" id="F2EHK4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0615060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0615060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0615060"/>
    </dbReference>
    <dbReference type="GeneID" id="123401784"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0509990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0509990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0509990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0615060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0615060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0615060"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401784"/>
    <dbReference type="HOGENOM" id="CLU_183267_0_0_1"/>
    <dbReference type="OrthoDB" id="668823at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051512">
        <property type="term" value="P:positive regulation of unidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900457">
        <property type="term" value="P:regulation of brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000024">
        <property type="term" value="P:regulation of leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080113">
        <property type="term" value="P:regulation of seed growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009646">
        <property type="term" value="P:response to absence of light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009741">
        <property type="term" value="P:response to brassinosteroid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044293">
        <property type="entry name" value="PRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR ILI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23174">
        <property type="entry name" value="bHLH_ILI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK06826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0615060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="88" mass="9838" checksum="9FAA555395049361" modified="2011-05-31" version="1">MSSRRSSRGAISDEEINELISKLQSLLPNSRRRGSSQASTTKLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAGAEIIRSILRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y2X1</accession>
    <name>M0Y2X1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123450812</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044983846.1">
        <property type="nucleotide sequence ID" value="XM_045127911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y2X1"/>
    <dbReference type="SMR" id="M0Y2X1"/>
    <dbReference type="STRING" id="112509.M0Y2X1"/>
    <dbReference type="PaxDb" id="4513-MLOC_65245.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0008140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0008140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0008140"/>
    </dbReference>
    <dbReference type="GeneID" id="123450812"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0006010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0006010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0006010"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0008140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0008140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0008140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450812"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0Y2X1"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y2X1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1071">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0008140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="510" mass="55773" checksum="BA0AE6A9FE795C7C" modified="2013-04-03" version="1">MEMERKPHAVLVPLPQQGHIAPMLKLAKLLHCKAGFHITFVHTEYNQRRLVRSHGPGALAGVPGFRFATIPDGLPPSDADASQDPASICYSTMTTCLPHFKKLLQELNATAGMPPVTCVVADNIMSFTLDAAAEVGVPCALFFTASACGYVGYRNFRFLMDKGIAPLKDEAQLTNGYLDTPVPDAPGMSQHMRLRDFPSFICTTDRGDVMLNFNLHEVERSGRAAAIIINTLDELEQASLDAMRAILPPPCPVYTIGPLHLLAQGQGQGQVLLPEIPEVLWKADGSCLEWLDGREPGSVVYVNFGSITTMSGEELVEFAWGLANCGHPFLWIVRKDLLAAKKDDDAAMQLPAEFLQATKGRCLLTSWCDQEAVLQHPALGVFLTHCGWNSALVAISAGVPMLGWPFFAEQQTNCRYASVEWGVGMEVGDNVRRQVVEARIREAMEGDGGNKLKRKAAEWKEICARAAPARSMANLHSLVKDVLMQPPPKTLDSSSSSCLEYEWNVPAHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y8M3</accession>
    <name>M0Y8M3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123411767</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044960667.1">
        <property type="nucleotide sequence ID" value="XM_045104732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y8M3"/>
    <dbReference type="SMR" id="M0Y8M3"/>
    <dbReference type="PaxDb" id="4513-MLOC_66731.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0685550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0685550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0685550"/>
    </dbReference>
    <dbReference type="GeneID" id="123411767"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0569140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0569140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0569140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0685550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0685550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0685550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411767"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y8M3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF441">
        <property type="entry name" value="S-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="162"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0685550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="426" mass="47716" checksum="A6F04F80D7B08CF1" modified="2013-04-03" version="1">MEGRMQGKVQQLSDSNRRIMEADAAPPLRVYQAWKGSNIFFLGGRLIFGPDVRSLIATVCLIVIPVIIFAAVVSPQLAHGYQNQIGGWVASVSIIFTAYILVLLLLTSGRDPGIVPRNSHPPEPEDIGESSNLSDWPGGQHGSAGLPLTKDVLVNGVLVKVKYCHTCMLYRPPRCSHCSICNNCVERFDHHCPWVGQCIGKRNYRFFLLFVSSATVLCIYVFTFCWVNIGKIMDTHECTFGRAILKSPISAILMLYTFIAVWFVGGLTSFHLYLISTNQTTYENFRYRYDRRSNPYNRGLVQNFIEILCSRIPSSRNNFRAKAKEDSAAFTSTLSMGRVLSPPKMSVDLEMGTKRQAVGAEDMEDLHSQIGSSMGLERCGTEPPHFDGRKGCSEIASDIETFAEEFGMDNKFTERKKIERHTNDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>M0YEA2</accession>
    <name>M0YEA2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ferredoxin--NADP reductase, chloroplastic</fullName>
            <shortName evidence="16">FNR</shortName>
            <ecNumber evidence="16">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123452561</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>2 reduced [2Fe-2S]-[ferredoxin] + NADP(+) + H(+) = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; photosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Plastid</location>
            <location evidence="2 16">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the ferredoxin--NADP reductase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="16"/>
    <dbReference type="RefSeq" id="XP_044985163.1">
        <property type="nucleotide sequence ID" value="XM_045129228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0YEA2"/>
    <dbReference type="SMR" id="M0YEA2"/>
    <dbReference type="PaxDb" id="4513-MLOC_6838.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0516480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0516480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0516480"/>
    </dbReference>
    <dbReference type="GeneID" id="123452561"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0429110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0429110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0429110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0516480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0516480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0516480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452561"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="UniPathway" id="UPA00091"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0YEA2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098807">
        <property type="term" value="C:chloroplast thylakoid membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06208">
        <property type="entry name" value="CYPOR_like_FNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000048">
        <property type="entry name" value="Ferredoxin--NADP reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000008">
        <property type="entry name" value="Ferredoxin--NADP reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015701">
        <property type="entry name" value="FNR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035442">
        <property type="entry name" value="FNR_plant_Cyanobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43314">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501178">
        <property type="entry name" value="FNR-PetH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000361">
        <property type="entry name" value="Frd-NADP+_RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 16" id="KW-0274">FAD</keyword>
    <keyword evidence="8 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 16 17" id="KW-0521">NADP</keyword>
    <keyword evidence="14 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="17">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="297"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="335"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0516480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="376" mass="41205" checksum="635F696720CBC65C" modified="2013-04-03" version="1">MATAAASQVAFSAPVAGSDRAVRSSGIKGTNSLSFGNKSWIGTALACDGKAALQQRHVCRAVQQSSKSKVSVAPLDLESAKEPPLNTYKPKGPYTATIVSVERAVGPNAPGETCHVVIDHGGNVPYWEGQSYGIIPPGENPKKPGNPQNVRLYSIASTRYGDSFDGKTASLCVRRAVYYDPETGKEDPSKNGVCSNFLCNSKPGDKIQLTGPSGKIMLLPESDPNATHIMIATGTGVAPYRGYLRRMFMEDVPNYRFGGLAWLFLGVANSDSLLYDEEFTSYLKQYPDNFRFDKALSREQKNKSGGKMYVQDKIEEYSDEIFKLLDGGAHIYFCGLKGMMPGIQDTLKKVAEQRGESWDQKLSQLKKNKQWHVEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287HSU7</accession>
    <name>A0A287HSU7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC123425685</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="RefSeq" id="XP_044965327.1">
        <property type="nucleotide sequence ID" value="XM_045109392.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965328.1">
        <property type="nucleotide sequence ID" value="XM_045109393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287HSU7"/>
    <dbReference type="SMR" id="A0A287HSU7"/>
    <dbReference type="FunCoup" id="A0A287HSU7">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287HSU7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134710"/>
    </dbReference>
    <dbReference type="GeneID" id="123425685"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0111000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0111000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0111000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0134710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425685"/>
    <dbReference type="InParanoid" id="A0A287HSU7"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287HSU7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000032">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000033">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000084">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF19">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="925"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="958"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="988"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="17">
        <location>
            <begin position="99"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="777"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="523"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="635"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="636"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="637"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="726"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="732"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="751"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="754"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="755"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="755"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1105" mass="124588" checksum="F8FB58BD0D3C88FF" modified="2017-11-22" version="1">MKRFVYINDESYQNDYCDNQISNTKYTLWNFLPKNLWEQFRRFMNQYFLLIACLQLWSLITPVNPASTWGPLIVIFAVSATKEAWDDYNRYISDKQANEKKVWIVKNGARKHIQAQDIRVGNIVWIRENEEVPCDLVLTGTSEPQGVCHVETAALDGEIDLKTRVIPTTCVGLDSEQLHKIKGVIECPIPDKDIRRFDANIRLFPPFIDNDICPLTINNTLLQSCYLRNTEWACGVAVYTGNETKLGMSRGVPEPKLTAMDAMIDKLTAAIFLFQLAVVVVLGSAGNVWKDTEARKQWYVKYDDDEPWYQILVIPLRFELLCSIMIPISIKVSLDFVKSMYAKFIDWDEEMYDQETDTPAHAANTAISEDLGQVEYILTDKTGTLTENKMIFRRCCIAGTLYGNESGDALKDIELLNAVANNSPHVIKFLTVMALCNTVIPIKSPGGTISYKAQSQDEDALVNAASNLHVVLVSKNGNDAEIHFNRRVIQYEILDILEFTSDRKRMSVVISDSQSGKIFLLSKGADEAMLPLAYSGQQIKTFVDAVDKYAQLGLRTLCLGWRELSLEEYLEWSRLFKEANSALVDREWKVAEVCQKLEHTLDILGISAIEDRLQDGVPETIEILRQSGINFWMLTGDKQSTAIQIALLCNLISSEPKGQLLYINGKTEDEVARSLERVLLTMRITSSEPKELAFVVDGWALEIILTRYKEAFTELAVLSKTAICCRVTPSQKAQLVKLLKSCDYRTLAIGDGGNDVRMIQQAHIGVGISGREGLQAARAADYSIGKFRFLKRLILVHGRYSYNRTAFLSQYSFYKSLLICFIQILFSFVSGIAGTSLFNSVSLMAYNVFYTSIPVLTTVLDKDLSEKTVTQNPEILLYCQAGRLLNPSTFAGWFGRSLYHAIVVFLITIHAYANEKSEMEELSMVALSGSIWLQAFVVTLEMSSFTFLQFLAIWGNFAAFYVINLCISTIPTAGMYTIMFRLCRQPSYWITMLLISGVGMGPVLALKYFRYTYSPSAINILQKAERSRGPMYTLVNLESQLRLEKDNSTNSILTTPVKNKSSVYEPLLSDSPMASRRSLASSSFDIFQPAQSRVPAAYPRNIKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287SPZ0</accession>
    <name>A0A287SPZ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123452905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044985575.1">
        <property type="nucleotide sequence ID" value="XM_045129640.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985576.1">
        <property type="nucleotide sequence ID" value="XM_045129641.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985577.1">
        <property type="nucleotide sequence ID" value="XM_045129642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287SPZ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0523330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0523330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0523330"/>
    </dbReference>
    <dbReference type="GeneID" id="123452905"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0435050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0435050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0435050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0523330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0523330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0523330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452905"/>
    <dbReference type="eggNOG" id="ENOG502T1VH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="681717at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287SPZ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF157">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0523330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="988" mass="112065" checksum="4956B35749687445" modified="2017-11-22" version="1">MRTEVIKADTIDEAIEGLLNELNKSRQNVIYFDGWEGLGACAVLRAIARCLELKEPSRPPGLDFEQVIHIDCSKWESTRALQREVAKQLKLSNWVMKMFDKQDEEDDFNGITDQGSRAEIADVAIEIQRSMQGRRFLAVLHNGGNEEIDISRLGLALYPYLTNKVIWTFQGRFRMDPKMKDKVTKNTTDVLLSASCSKCDPRVLWFYLLDKEAAHVACKHGISLTIVVRCFLCLLDLNCIRGHHVDTEYDLTIHTCNYWICDGIIPKIPGCGEEAWQDGENLQGEIRLDMHYHQNDLPPHLLMYNEKRPHWTSPAYGYVLVQDRVVPSGMLQHFDKLSVIKLSSCTFSFSSPPFLCCHSLRFLCLEHCQDLRKTYAEQREEEEDTTRSRACFQSLWVLDLRYTDCDWILSVRVMDLMTQLRELIVMGAKDWHMNHLRGRLRNIQKLRVTKSTCFFNGNLLSEMESIELLDFSGNIVNGGTSSLSGRASNSSLSGPASNSSLKTVFIDGCDGLGKISFGGCKELKNLFLKGLLRGLEELDISYTGVKTLDLGEVRASTLPKRFILVGCEKLRAILWPQGAEKWYGVLFIHQTSALASSNGWEEPHADRSLQQQKEKKFEFGSRISLMDTRLLRSLSAVRICLENSSVHIDIYTAATVGRSSVNLPQAQPHTGITMDSMYIDVLKDGPVTAMMMWGCPEILPGQPLQTCIMKVILHGHGSKTLEDAATSSTTSALSLPEFIFQVVRSLHMYDNSSTTTILAPPKDPAWTNLRWCRVERCPALRTVFNVPQDSDRSSFSLLETFWASQLPSACYIWTIASVGTFSCLTLLHLDRCPRLIHVLPLSTAPGSCLSSPMSPPCLFRLETLEIVYCGDLREVFPLSPELREQDTPILFPQLRRIHLHGLPSLQRICGRRVFAPKIETIKIRGCWSLRRLPGVRINTAKPPKVDCEKDWWDNLEWDGVKENHHPSLYEPSHSLYYKAKVPRDTVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WGQ3</accession>
    <name>A0A287WGQ3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123409774</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044958578.1">
        <property type="nucleotide sequence ID" value="XM_045102643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287WGQ3"/>
    <dbReference type="FunCoup" id="A0A287WGQ3">
        <property type="interactions" value="1724"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287WGQ3"/>
    <dbReference type="PaxDb" id="4513-MLOC_4792.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0680770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0680770"/>
    </dbReference>
    <dbReference type="GeneID" id="123409774"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0565100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0565100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0565100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0680770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0680770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0680770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409774"/>
    <dbReference type="eggNOG" id="ENOG502QS71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287WGQ3"/>
    <dbReference type="OMA" id="APPIFIH"/>
    <dbReference type="OrthoDB" id="514967at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287WGQ3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1100.10:FF:000001">
        <property type="entry name" value="Squamosa promoter-binding-like protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF196">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26102">
        <property type="entry name" value="Ig_SPL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1090"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="131"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="549"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0680770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1130" mass="123727" checksum="B3FE47375AE40EB9" modified="2017-11-22" version="1">MQREVGPQVASPLYLHQIQPLPPHAAAAARKRGTPWPAADPPENAAMGAGAAAGGNWNPSMWDWDSRAFTARPSSDALRLGGGLNHHQHHHQQPPPPPPPATAAEAQRQGRGGAGDLSLQLNLREEASMAMDVSPTTTMSSSPSPPARTSQEQAARPSKRVRSGSPGTASGGGGGGGAGGSASGGGSYPMCQVDDCRADLTSAKDYHRRHKVCEIHSKTTKAVVANQMQRFCQQCSRFHPLSEFDEGKRSCRRRLAGHNRRRRKTQPTDVASQLLLPENQENTANRTQDIVNLITVIARLQGGNVGKLPSIPPIPDKDNLVQIISKINSINNANSLGKSPPSEAIDLNASHGQQQDSPVQNATKVVDKQTVPSTMDLLTVLSGALGTSTPETNTSQSQGSSDSSGNNKSKSHSTEPACVVNSHEKSIRPFPAAGVIRSNSTHGSPPEIYKQPDRDTHPYLSLQLFGNAEVDIPVKMDTANKYLSSESSNPMDERSPSSSPPVTRTFFPTRSVNEGIRHPRIADYGEDAATAEISTTRAWCAPQLELFKDSERPTENGSPPNPTYQSCYASTSGSDHSPSTSNSDGQDRTGKIIFKLFGKEPGSIPGNLRDEVVNWLKHSPTEMEGYIRPGCLVLSMYLSMPTIAWDELEENFLQRVNSLVQASDLDFWRKGRFLVRSDNQLVSYKDGMTRLSKSWRTWNTPELTLVTPIAVVGGRKTSLVLKGRNLTIPGTQIHCTSGGKYISKEVLCSAYPGTIYDDSGVETFDLPGEPNLTLGRCFIEVENRFRGNSFPVIFASKSICHELRNLEAELEDSRFPDVSSDDQVHDARRLKPRDQVLHFLNELGWLFQKAAACTPSIESDVSDSELIQFSTARFRHLLLFSNERDWCSLTKTLLEVLSKRSLVSEELSQETLEMLSEIHLLNRAVKRKSSHMVHLLVQFVVICPDNSKLYPFLPNYPGPGGLTPLHLAASIDDAEDIVDALTDDPQQIGLSCWHSVLDDEGISPEVYAKFRNNGSYNELVARKLVDRKNSQVTIVLNKGEIHMDQPENAGANNSSGIQALEIRSCSQCAILESGLLRRPMRSRGLLARPYIHSMLAIAAVCVCVCVFMRALLRFNSGRTFKWERLDFGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WV16</accession>
    <name>A0A287WV16_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123410061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958880.1">
        <property type="nucleotide sequence ID" value="XM_045102945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287WV16"/>
    <dbReference type="SMR" id="A0A287WV16"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701320.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701320.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701320"/>
    </dbReference>
    <dbReference type="GeneID" id="123410061"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0581770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0581770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0581770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0701320.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0701320.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0701320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410061"/>
    <dbReference type="eggNOG" id="KOG2741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="446809at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287WV16">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050424">
        <property type="entry name" value="Gfo-Idh-MocA_inositol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43593:SF1">
        <property type="entry name" value="INOSITOL 2-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="167"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0701320.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="387" mass="42177" checksum="93173F0627E30B2E" modified="2017-11-22" version="1">MAREGGGAAMAPAAAVVRYGIVGVGMMGREHLHNLAHLAGEVEWEQSVRVCVTCLADPHPESLLLGLRLAAELGLPPPQTFSDHRELLDSGLCDAVVVSSPNMTHSEILMDIIGYAKPHHVLVEKPLCTTVQDCEKVIEAAKQRPDILVQVGLEYRYMPPVAKLIDTVKSGTLGQVRMVAIREHRFPFLVKVNNWNRFNCNSGGTLVEKCCHFFDLIRLFADANPVRVMASGAIDVNHKDEVYHGKVPDIIDNAYVIIEFDNGCRGMLDLCMFAEGSRNEQEISVVGDTGKGEAFVPESIMRVGKRAGGRDGVVTIKAEDERIKYQGLHHGSSYLEHLNFLSAIRSQGACCPAISLEDGLLSVAIGVAGQLSIEKGRFVTIEEVLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287WXD8</accession>
    <name>A0A287WXD8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123409754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044958552.1">
        <property type="nucleotide sequence ID" value="XM_045102617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287WXD8"/>
    <dbReference type="FunCoup" id="A0A287WXD8">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287WXD8"/>
    <dbReference type="PaxDb" id="4513-MLOC_11994.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704050"/>
    </dbReference>
    <dbReference type="GeneID" id="123409754"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0583930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0583930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0583930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0704050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409754"/>
    <dbReference type="eggNOG" id="ENOG502QS92">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287WXD8"/>
    <dbReference type="OrthoDB" id="8707547at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287WXD8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019376">
        <property type="entry name" value="Myeloid_leukemia_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13105">
        <property type="entry name" value="MYELOID LEUKEMIA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10248">
        <property type="entry name" value="Mlf1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="36091" checksum="4556C7537C39337A" modified="2017-11-22" version="1">MQRGRGGRGGLFGFGDPFPAFGGFVPPGNLMSSFFGGSNPFDDPFFTNPSGSMIGPSLFEQSFFGSSMFGPQRALNGGGFQQQGPEPSRPKGPIIEELSSDGEDGADANEHDKKKKTNSMKHPRISKEPYVEDPDEEVQDNKRPRHEKIGKEYVRAGTSYHQPQTYMFQSSTVTYGGSNGACYMSSTTRRSGGDGVTMEESKEADTTSGKATHRIARGIGNKGHALTRKLNCDGKVNSMQTLQNLSEDELAGFDESWQRNAGSCLPGWDPRLNMLNSDKAGALSPAIQEDNGMSVLPTPNEVFALPAPEQYRGSNSSRMKRCPLNGSSLGSPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W6U3</accession>
    <name>A0A8I6W6U3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123400080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045094483.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6W6U3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0065820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0065820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0065820"/>
    </dbReference>
    <dbReference type="GeneID" id="123400080"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0052550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0052550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0052550"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0065820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0065820"/>
    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0065820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="494" mass="53452" checksum="3789B49F86A3965E" modified="2022-05-25" version="1">MSVKDEQHQPLHILFFPFPAHGHLIPIADMAALFAARGVRCSILTTPVNAAIVRSAVDHANDAFAGTNSPAIDISVVPFPDVGLPPGVENGTAIKSQGDRGKFFLAAQLLREPFDRFLFDNRPDAVVSDSFFPWSVDAAAEHGVPRLAFLGSSMFALSCIDSMLRHNPLATVPDDPDALVSLPGLPHHVELRRSQMMDPANQAEHWGWAFFEGMDAADRRSFGEVFNSFHELEPDYVEHYHTTLGRRAWLVGPVALATKDMAGRGTDALSPDAANCIGWLDMKPASSVVYISFGTLTSFSPAELHQLARGLELSGMNFLWVIGAAGPDTSEWMPEGFAELMAHGDRGFIIRGWAPQMLILNHPAVGGFLTHCGWNSTLEAVSAGVPMVTWPRYADQFNNEKLIVEVLKVGVSIGAKDYASCVETHEVIDGEVIAESIGRLMGNGEEGDAMQKKAKDLGGKARRVVENGGSSYNAVGRLIDELTARRSSVKVGGK</sequence>
</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0107220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="118" mass="14442" checksum="622F054C6360B0FB" modified="2022-05-25" version="1">MYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYCVEQRAFLDEMIRKNQEEKLRLEEVYRQREQAREAERERKRERARAAKAAEEAGDGKGKYPRSTQ</sequence>
</entry>
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    <accession>A0A8I6WHZ1</accession>
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    <protein>
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            <fullName evidence="3">Guanylyl cyclase</fullName>
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        <lineage>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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        <source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965152.1">
        <property type="nucleotide sequence ID" value="XM_045109217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WI52"/>
    <dbReference type="SMR" id="A0A8I6WI52"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130510"/>
    </dbReference>
    <dbReference type="GeneID" id="123425528"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0107680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0107680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0107680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0130510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0130510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0130510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425528"/>
    <dbReference type="OMA" id="DFFCFFR"/>
    <dbReference type="OrthoDB" id="609103at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000146">
        <property type="entry name" value="putative MO25-like protein At5g47540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF12">
        <property type="entry name" value="OS07G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0130510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38827" checksum="3AD09128E31C4D5F" modified="2022-05-25" version="1">MKGLFKSKPRTPADVVRQTRELLIYVDLHAGTSGADPKREEEKMAELSKNIRELKCILYGNGEHEPVTEACVQLTQEFFRENTLRLLIMCVPKVNLETRKDSTQVVANLQRQQVNSRILASEYLEANKDLLDTLISGYEDTEVALHYGAMLRECIRHQSIARYVLESDHMKKFFDYIQIPNFDIASDASATFKELLTRHKATVAEFLSKNYDWFFAEFNSRLLSSSNYITKRQAIKLLGDMLLDRSNSAVMMRYVGSKDNLMILMNLLRDSSKNIQIESFHVFKLFAANKNKPAEVVNILVTNRSKLLRFFAGFKTDKEDEQFEADKEQVIKEISAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WL14</accession>
    <name>A0A8I6WL14_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WL14"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0115130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0115130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0115130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0115130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0115130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46934">
        <property type="entry name" value="MYB_DNA-BIND_3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46934:SF11">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0115130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="256" mass="30309" checksum="431118643B0CDA87" modified="2022-05-25" version="1">MEHDTPYHRGQNGWSKETWNLMVTKFHAKHKHVKFWKSQIQHKEKELKREYRMLKEARKQSGVGWDDKRCMIEADTDLWDNLMISYPSIGKFKKKSFPLFDSLRELYDRHTTEGTYSFTSIAQPSQIDEDFEDEREVEEVKESDDLEMMNQVQHDEDDLQILDQMDVAHRKEDVNPSEELGRTMARSGKMPQKKPTKEKRNNSGDVIAGALEKYIELKKRQVDDEATYLANEKAEGTKLHEFSITKCMDVSKKWRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WM59</accession>
    <name>A0A8I6WM59_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123442440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974458.1">
        <property type="nucleotide sequence ID" value="XM_045118523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WM59"/>
    <dbReference type="SMR" id="A0A8I6WM59"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005220"/>
    </dbReference>
    <dbReference type="GeneID" id="123442440"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0004000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0004000.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0004000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005220.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442440"/>
    <dbReference type="OrthoDB" id="604685at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010208">
        <property type="term" value="P:pollen wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018088">
        <property type="entry name" value="Chalcone/stilbene_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF73">
        <property type="entry name" value="CHALCONE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00441">
        <property type="entry name" value="CHALCONE_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="241"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005220.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="43278" checksum="5172F166B93647CC" modified="2022-05-25" version="1">MAAAVKLEEVRRAQRAVGPATVLAIGTAVPANCVYQATYPDYYFRVTKSEHLSDLKEKFERMCEKSTIRKRHMHLTEDILKKHPSICSHMEPSLNTRHDIVVVEVPKLGKEAAERAIKEWGQPLSKITHVIFCTTSGVDMPGADYQLTRLLGLSPTVKRLMMYQQGCFGGATVLRMAKDIAENNRGARVLVVCSEITAMAFRGPSKSHLDSLVGHALFGDGAAAAIIGADLDEPFEKPLFQLVSASQTILPESEGAINGHLTEAGLTIHLLKDVPGLISQNIEQALEDAFKPLGIHDWNSIFWIAHPGGPAILDMVEEKVGLDKERMRASREVLSEYGNMSSACVLFVLDVMRKTSSQDGHTTTGEDKEWGVLFGFGPGLTVETLVLYSAPIIATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WPF2</accession>
    <name>A0A8I6WPF2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
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        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WRH0"/>
    <dbReference type="SMR" id="A0A8I6WRH0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0033120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0033120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0033120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0033120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0033120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0033120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF442">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0033120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="211" mass="23374" checksum="2D61954D15BB03CD" modified="2022-05-25" version="1">MRADLERLGHVSSDNGGFSLHDLGQLLSSGEGADVTFEVGGETFAAHRCILGARSFVFRAELLGPMKESTSTCVQIEDMEPKVFKALLHFIYTDSLPEIDEAEALEMIQHLLVAADRYGLKRLKLTCEEKLCSYINTTTVATTLALSEQHACPALKEECLRFLESSNNSTLDLITRSSDFEHLATSCPSIMKELIPKLARKPPFVINYSNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSY6</accession>
    <name>A0A8I6WSY6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123447801</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044980419.1">
        <property type="nucleotide sequence ID" value="XM_045124484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSY6"/>
    <dbReference type="SMR" id="A0A8I6WSY6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079130"/>
    </dbReference>
    <dbReference type="GeneID" id="123447801"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0064460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0079130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447801"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03564">
        <property type="entry name" value="ANTH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000005">
        <property type="entry name" value="Clathrin assembly protein AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000003">
        <property type="entry name" value="Putative clathrin assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF89">
        <property type="entry name" value="OS05G0549000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="24"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="565" mass="62619" checksum="9C8789034A0E1B12" modified="2022-05-25" version="1">MASMQSWRKAYGAIKDTTTVSLANINSDFKDLDVAIVKATNHVECPPKERHLRKIAAATSIARPRADIAYCIHALSRRLSKTRNWIVALKTLVVVHRLLREGDPTFREELLNFTQRGRILQLSNFKDDSSPIAWDCSAWVRTYGQFLEERLECFRILKYDVEAERLSKQGQGPEKGHSRTRDLDSQDLLEQLPALQQLLYRLVGCRPEGAANNNYLVQYALALVLKESFKIYCAINDGIINLVDKFFEMARHEALKALEIYRRAGQQAGNLSDFYENCRGLELARNFQFPTLREPPQTFLVTMEEYVRDAPRMVPVREPLEFPERLLLTYKPEEAEEVSVPDPVEEEQPPVEEPVPVQSVAEVVSPPSKPVVADTGDLLGLNDPSPAVSAIEESNALALAIIPSDPSTSTTSSTTWQDKGFDPTGWELALVTAPSNTNSSVTDSQLGGGLDKLILDSLYDEGAYRQSQQQQLYGSSAPNPFMTNDPFAMSNHVAAPSSVQMAAMSQQQQQIPTMIHQNPFGPPIQPQHPGAGPAAVNPFLDSGFGAFPAANNQQQQANPFGGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTP7</accession>
    <name>A0A8I6WTP7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTP7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0143820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0143820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0143820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0143820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0143820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0143820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0143820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="105" mass="11958" checksum="70E4AD4E2E2E4E2D" modified="2022-05-25" version="1">MRLMQTYNADERPTGVGSQPTAAQQRSDTGANQTPWRGVVTTCGNQAVVLFALYLISTKSRSGVDCFLFLFWAFTREWGHETSVDWGPFRAVLDMYRDQRTNMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTW8</accession>
    <name>A0A8I6WTW8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123449061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044982082.1">
        <property type="nucleotide sequence ID" value="XM_045126147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTW8"/>
    <dbReference type="SMR" id="A0A8I6WTW8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083250"/>
    </dbReference>
    <dbReference type="GeneID" id="123449061"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0068360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0068360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0068360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0083250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0083250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0083250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449061"/>
    <dbReference type="OrthoDB" id="60843at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="257"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0083250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="500" mass="51322" checksum="FFAC7F01D512A866" modified="2022-05-25" version="1">MAELPLAGGLLDLRPGKLSPKPPPLPMPCRRSPPRPQVAAAVASPCRAVPELHSTTELADGSVVFHFARPPVKDPAEPTASGPGPGHADASASRPELRVDEPNPHAAADAAEISLASPEAPQQAVSSASAGVEAEAGLVSGRAVPPADCQLETDGNGLELGALEESETVEAGTAAPTGLEEVDADAGASSERAITLDFDTAVGADSRGDDQGATESGATIPPPAGEVHNNVDCEKNTSEVKSSDRTVPVASSALLLTSGAAILPHPSKVATGGEDAYFIEHNGWFGVADGVGQWSFEGINAGLYARELMDGCKKVIAESGGGCELAPEQVLSKAAAEARSPGSSTVLVAHFDGQLLHASNIGDSGFLVIRNGEVHAKTKPMVYGFNFPHQIEKGVDPLTLVENYVIEIEEGDVIITATDGLFDNVYEQEAAAIISKSLQADLKPAEMAQHLAARAHEVGRSGAGRSPFSDSAVAAGYLGFSGGKLDDTAVVVSIVRRSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZ29</accession>
    <name>A0A8I6WZ29_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123439434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972083.1">
        <property type="nucleotide sequence ID" value="XM_045116148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZ29"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0234060.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0234060"/>
    </dbReference>
    <dbReference type="GeneID" id="123439434"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0194260.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123439434"/>
    <dbReference type="OrthoDB" id="663108at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34278:SF11">
        <property type="entry name" value="OS01G0510200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34278">
        <property type="entry name" value="PROTEIN THI031, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0234060.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="169" mass="18919" checksum="3CCDD6AB3861F1F4" modified="2022-05-25" version="1">MRREGRQRGWVRVYDRALVDPDGNRRAVHVVDGPAVANGGYIRAPRKPTNQSKSGGLRTLGRDSSVAQEDEEEEPQPHPPLGERPSAAGYYCRTTCQSPFKLQEVPRRWTYYAFVPEQAPSRPATAARSGGRASCKGGRKFKHNEVKMYYVDAADDAEGRLDYLCDFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0F1</accession>
    <name>A0A8I6X0F1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0F1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0237890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0237890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0237890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0286600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286600.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286600.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="145" mass="16729" checksum="4D62BA25678515DF" modified="2022-05-25" version="1">MNYNDPTFCSLPTVSERRYPDGVVVETMLGVWVISRWRGSRELAHAFLRAGFGDLSAGSPVMFHLEEMRPNASSPATTLLANFTNRFDAFYLLQEVFWCGCKFIAFTTYNIFTDVESIFPIVNAMHNLPYYIGENGMEEDYLRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3U3</accession>
    <name>A0A8I6X3U3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptidase M50 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123442612</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable membrane-associated metalloprotease that may be involved in chloroplast development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974637.1">
        <property type="nucleotide sequence ID" value="XM_045118702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3U3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227980"/>
    </dbReference>
    <dbReference type="GeneID" id="123442612"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0189070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0189070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0189070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0227980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442612"/>
    <dbReference type="OrthoDB" id="5738at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06160">
        <property type="entry name" value="S2P-M50_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF5">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="15">
        <location>
            <begin position="379"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="621" mass="65142" checksum="9602D995AF4E0904" modified="2022-05-25" version="1">MGSSSCPPPSQATSSYGCCCHRPLLASTSAPARSRGARLTLSLTLRSPSLPLPAGPLSRSRSRRVACRAADEPEPAASNGDEEKKGAVDDAPSPPADSVVQADATAETGSGADNTKDESPNAEQLNSAATSLQKIEAYVSAAADSAAQTKGESPKADRLNSALASLEKFQGYANAAADSAAQTKDESPNAEPVSASDTAQNIGRDATAAADTAAQEQVVEADVNVASGSPLPGMKQQLEEAVSIPKATVDILKDQVFSFDTFFVTSHEPYEGGILFKGNLRGVPAKSFEKITTRLENKFGDEFKVFLLINPEDEKPVAVVVPKQTVEPATGSVPEWAAAGAFGLVTIFTLLLRNVPVLQDNLLSTFDNLELLKDGLPGALVTGLIIGVHEIGHILAAKDAGVKLSVPYFVPSWQIGSFGGITRIVNIVRNRGDLLKVAAAGPVAGFSLGFVLLLLGFILPPSDGLGIIVDPAVFHQSFLLGGLAKLILGDVLKEGTQLSINPLVLWAWAGLLINAINSIPAGELDGGRIALAMWGRKVSSRLGSVTIALLGLSSLFSDVAFYWAVLIFFLQRGPIAPLSEEITEPENSYIGLGVAILFLGLLVCLPYPFLFDPSQVTDFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7Q0</accession>
    <name>A0A8I6X7Q0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV radiation resistance-associated gene protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X7Q0"/>
    <dbReference type="SMR" id="A0A8I6X7Q0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0056150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0056150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0056150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF22">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40285" checksum="7DF70B416A84D343" modified="2022-05-25" version="1">MSPRAGPDPGAEASVAWPDLRGRLEQAGALAAQLAAAAEERARLARRLQAALQVRRESLRQGAALDEMRRRVERRRARADDLAVARRRAAEGVERQKEQMQAQIERVLLLSRALAAAHRQVQEAKELKSAEKARLGDLQRLLRTRQQSMVAQVAALYPVRVFRELPVAQNHHSRTNGERQTPSQENGALPHENGNGIHLLSAIKSPHVGPFTFFGWQIGKPKTKQPSYSHKELQRSAAVLGYAAHAVLLIASYLDIRLRYPLRFGGSRSYIGDRLPSAETSSIASVEHRSIDSADSKLTDYPLFLEFQDDSTKASYAIYLLHKDTEQLLNYIGAECSGRNVFGNLRELIRIILSDEYLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9A7</accession>
    <name>A0A8I6X9A7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9A7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0283930.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0283930"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF36">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN 1-LIKE"/>
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    </dbReference>
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            <end position="236"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0283930.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="423" mass="47998" checksum="A9C1B3DC400639A6" modified="2022-05-25" version="1">MKNKQGNDLKRLWDRAASKAPKISSTSTPASVSIDVENQDQDHNFSNPPSNELQLALVQTSNNSGMEPEIGSPDVEDEESDEEFNDEAVYVLEPDPGKRIPIVNYDVNDQDLVRRRYIAMGACQPKKHEFKTTKNGGNNCRFSAHWFDTYEWLEYSVKLDAMFCFVCYLFKDKTKLKGGDAFVKGGFKNWNMMKNRCEKHCGSLTSAHCEAQEKFDLFIKPDASIRESLASTSKQAKAQYISRLGYSIYCLRFLLKQGLAFRGHDESEESLNRGNFLELLNHLAEKFEDVDKVVLENAPKNCKMTSHDIQLELINCCAKETTSLIMKDLGEEYFAILADESSDVYQREELALCLHYVDKIGWPVERFLGIVHVEDTTSLTLKKAIEKLLMDNSLSFSMVRGHGYDAASNMKGKANGLKKFNYG</sequence>
</entry>
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    <accession>A0A8I6X9Z8</accession>
    <name>A0A8I6X9Z8_HORVV</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9Z8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221960.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.3HG0221960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0184540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0184540.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0184540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0221960.1">
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    </dbReference>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040274">
        <property type="entry name" value="CLE27/CLE43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37184">
        <property type="entry name" value="CLAVATA3/ESR (CLE)-RELATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37184:SF2">
        <property type="entry name" value="CLAVATA3_ESR (CLE)-RELATED PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0221960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="145" mass="14688" checksum="FA945A6273DE816A" modified="2022-05-25" version="1">MQPAQPPCGSRRGRGAPAVPRAPLPVPRRRLLLVVVIVYLVGGSDVLPLTAAAAPARRLGAAAFVEYDVVGTRPIPGDVLPLTAAVAPARRLGAAASVGYDVVGTRPIPGEAVASSPSPSSTPSAVGLADNKRPVPSCPDALHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBW1</accession>
    <name>A0A8I6XBW1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123445486</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the plant 'ANKYRIN-BTB/POZ' family. 'NOOT-BOP-COCH-like' (NBCL) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045122474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBW1"/>
    <dbReference type="SMR" id="A0A8I6XBW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0319900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0319900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0319900"/>
    </dbReference>
    <dbReference type="GeneID" id="123445486"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0266940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0266940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0319900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0319900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0319900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445486"/>
    <dbReference type="OMA" id="RMKDEHT"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009864">
        <property type="term" value="P:induced systemic resistance, jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18310">
        <property type="entry name" value="BTB_POZ_NPR_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000084">
        <property type="entry name" value="regulatory protein NPR5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000058">
        <property type="entry name" value="regulatory protein NPR5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044284">
        <property type="entry name" value="NPR5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024228">
        <property type="entry name" value="NPR_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057250">
        <property type="entry name" value="Znf_C2HC_NPR-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46668">
        <property type="entry name" value="BTB/POZ DOMAIN AND ANKYRIN REPEAT-CONTAINING PROTEIN NH5.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46668:SF1">
        <property type="entry name" value="REGULATORY PROTEIN NPR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11900">
        <property type="entry name" value="DUF3420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52046">
        <property type="entry name" value="ZF_C2HC_NPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="16">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC NPR-type" evidence="17">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="349"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="486"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0319900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="513" mass="54143" checksum="8F8C30B136E5C1B0" modified="2022-05-25" version="1">MDDTFKSLSMDYLNLLINGQAFSDVTFSVEGRLVHAHRCILAARSLFFRKFFCGAAADQAAAAAAAGSPGAVLMDHLSPRSPSGASASSPRGAGGSGSASAAAMAPGAVIPVNSVSYEVFLLVLQFLYSGQVSLVPQKGEPRPGCGERGCWHTHCAAAVDLALDTLTVARSFGVEELALLTQKQLAGMVEKASIEDVMKVLMASRKQDLHQLWNTCSHLVAKSGLPPEVLAKHLPLDVVAKIDDLRLKSSMSRRSPFLAHHQQHQQHHHQGSVIDASAAELDDHNKIRRMRRALDSSDVELVKLMVMGEGLNLDEALALHYAVENCSREVVKALLELGAADVNHPAGPAGKTPLHVAAEMVCPDMVAVLLDHHADPNVRTVDGVTPLDILRTLTSDFLFKGAVPGLTHIEPNKLRLCLELVQSAAMVMSREDAAGNAMVPMYSDHHPGAGGGGVYSGTGGTSTSMNLSLDNRMVYLNLGMDVMNHGDGGGGDGGGSRSGQGGPSSLFSPHGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XEJ5</accession>
    <name>A0A8I6XEJ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123448451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. ABCF family. EF3 (TC 3.A.1.121) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981283.1">
        <property type="nucleotide sequence ID" value="XM_045125348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XEJ5"/>
    <dbReference type="SMR" id="A0A8I6XEJ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0365270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0365270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0365270"/>
    </dbReference>
    <dbReference type="GeneID" id="123448451"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0304230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0304230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0304230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0365270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0365270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0365270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448451"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000309">
        <property type="entry name" value="ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000011">
        <property type="entry name" value="Putative ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051309">
        <property type="entry name" value="ABCF_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000355">
        <property type="entry name" value="ribo_prot_ABC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42855">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42855:SF1">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="98"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="425"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="651"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="680"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0365270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="692" mass="77398" checksum="489EE8B4CE2E93A9" modified="2022-05-25" version="1">MATMDVISAKLLRSSLHPHAPFLSCPSLPSRRHRRPSSIYCRLSTSSLSSSSAATTSEGDANEHLSALLSDESTTATSGAGSRKKRSNSGASSIPSGVRLENISKSYKGVTVLKDVSWEVQRGEKVGLVGVNGAGKTTQLRIIAGLEEPDGGNVVKAKENMKIAFLSQEFEVCASRTVREEFLSAFQEEMGVKTRLDQVQAALERATEDMDLMGRLLDELDLLQRQSQDVDLGMVGVKIQKLMPELGFVPEDADRLVASFSGGWKMRMSLGKILLQDPDLLLLDEPTNHVDLDTIEWLESYLKTQDVPMVIISHDRAFLDQCCTKIVETEFGVSKTYKGNYSEYVLAKAIWVETQRAAWEKQQKEIEHTRELIGRLGAGASSGRASSEQKKLEKLEKEGLIEKPFQRKQLKISFPERGRSGRTVLAINNLKFGFGDKILFNNANLIVERGEKIAIIGPNGCGKSTLLKLALGTEKPQEGEVILGEHNVLPNYFEQNQAEALDLEKTVLDTVAEAAEDWKIDDIKGLLGRCNFRDDMLNRKVRFLSGGEKARLSFCKFMVTPSTLLILDEPTNHLDIPSKEMLEEAISEYTGTVITVSHDRYFVKQIVNRVIEVKDQTIQDYQGDYNYYLQRNLEARERELARAAELEEKAPKVKAKSKMSKAEKLARKKQKVQAFQQSKQKSKSMKNSKRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHH9</accession>
    <name>A0A8I6XHH9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123446316</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHH9"/>
    <dbReference type="SMR" id="A0A8I6XHH9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0301890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0301890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0301890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0362350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0362350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0362350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001625">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF540">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="226"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="334"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0362350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="61285" checksum="89FECBE808ACA602" modified="2022-05-25" version="1">MKGCPLPQGKQKTMPFGKTKRKIQPKPSCLALLPMPSTAAASEPSPSPPHAVTTPDGWHPRTTERRLLHLLHHSSRARRRPLELLAFAVRHCLHSSPPSPHHHFLAALLLLSSPPPPALSLLSLLPPEPPPPLPLLNAALKSLCASSPPLAFRLLSSLRRLHAPDRLSFLPLLGPTSSLPLLSAFHGLLLRLGFLSHHAISLALLRPYRLPHVRILFDEMPQQRKCTIAYNTLITAYVKAKDIFTARHLFDEMQRFKRSRRSMVSWNAMISGCTWCGRDDMAVRYFQDMVREGKVAPDDGTLAAVLPACGRIGNAGAGRWAHEYACKKGILDISVHVANAVVDMYCKCGVVNSAREVFEGMQQRSVVSWNTMISGFSLNGQGIKGIELFRQMVRSGGEPNSVTFLGVLGCCAHAGAVDTGQLIFQTMQSEHGIEAGIEHYGCMVDLLGRSGLLEEAHALIQGMRMSPNSAIWGSLLSACRAHAGLGIAEVALKELISLEPWNSGNYMLLANLYAETQRWDEAGDVRKLMRRMSVQKAPGQSLIEEPSSSWQIHDHIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHU0</accession>
    <name>A0A8I6XHU0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123399899</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950218.1">
        <property type="nucleotide sequence ID" value="XM_045094283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHU0"/>
    <dbReference type="SMR" id="A0A8I6XHU0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495950"/>
    </dbReference>
    <dbReference type="GeneID" id="123399899"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0495950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0495950.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0495950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399899"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000037">
        <property type="entry name" value="ATPase family AAA domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="914"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="853"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1061"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="853"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1065"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1118"/>
            <end position="1130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0495950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1211" mass="134746" checksum="80F11F1A0026BB92" modified="2022-05-25" version="1">MAGMAGKGDDSVAPVRSSDRLRQRTKYYSRGYLYYSPSMRNKMKSNKKRTAASQIAKKLLGKPAAREPPADSIAANLRRSTRKRRMSVTLEDYGTDSSSMEDDDLMRPRYRSSSKSKGDDQVSSRPKCKKMSNSNSIPRREGLRPRRSLRGQRHLPYQVSDDDQESSEEEHEQDQRENGNEVEEDGANEEEIDGGDEAEEDGGDEAEEDGDDEDGEEEQEVRRRYDLRDRAEVRRPSPQKEGKHRPQSPRRVLVQGIGPKNSKYLKKGGSRTHKRPRFSVPDDSDDSLLVDEPDEGPSMPWMRSGRGGMPPWLMGGLDMHNSTAWGLNAGASGWGHQGDTGVSTSSLMPGAQTAGPSSKGGADIQPLQIDESVSFNDIGGLSEYIDALKEMVFFPLLYPDFFANYHITPPRGVLLCGPPGTGKTLIARALACAASKAGQKVSFYMRKGADVLSKWVGEAERQLKLLFEEAQKNQPAIIFFDEIDGLAPVRSSKQEQIHNSIVSTLLALMDGLDSRGQVVLIGATNRIDAIDGALRRPGRFDREFYFPLPGYEARAEILDIHTRKWKEPPPKELKMELAASCVGYCGADLKALCTEAAIRAFREKYPQVYTSDDKFVIDVDSVRVERNHFLEAMSTITPAAHRGAIVHSRPLSPVIAPCLKRHLEKIMQRISDTFPYLSALDISKYSTLSYGSSIPLVYRPRLLMCGVEGVGLDHVGPAVLHELEKFPVHSLGLPSLLSDPSAKTPEEALVHIFGEARRTTPSILYLPQFHLWWDTAHEQLRAVLLTLLNELASNLPVLLLGTSSVAFDDLEEECASIFSSRNVYQVDRPSDDDRSRYFSILFESLLSLQMEDSRSKSKEQKSVDLPKAPKKVDGPKVSELKAKAEAEQHAVRRMRMCLRDICNRILYNKRFSAFHFPVSEEEVPDYRTIVHNPMDMAAVLQRVDSGQYFSQAAFLKDINLIVTNAKTYNGDDYNGSRIVSRACELRDVVQGMLSQMDPSLVSFCDKIAAQGGPLQAMDDEDSSILQAPPVVQLVSVTRTSARLRNVQPEVALSRSYEVLKRHKKSTENDHGTAAKESTARDERSPGDVDLSKPTSPEEAPKGSHPNGPSKKADKAPAESPPILPGSPPDPMETDNGEDSAMPTSDDTLEQLEGVKQRFMELTVGYGVPQLERLYSRIMRGAIELTGKETNKDHRRLVVRHLLAFVENSDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XM58</accession>
    <name>A0A8I6XM58_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123450069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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                <consortium name="EnsemblPlants"/>
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    </dbReference>
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    <dbReference type="SMR" id="A0A8I6XV24"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275170"/>
    </dbReference>
    <dbReference type="GeneID" id="123443961"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0227990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0227990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0227990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0275170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443961"/>
    <dbReference type="OMA" id="MINEDPR"/>
    <dbReference type="OrthoDB" id="1078367at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090297">
        <property type="term" value="P:positive regulation of mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000160">
        <property type="entry name" value="single-stranded DNA-binding protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF16">
        <property type="entry name" value="NUCLEIC ACID-BINDING, OB-FOLD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="23877" checksum="1281E68A97B4235A" modified="2022-05-25" version="1">MAAATSSSLLGRRFLLLSRRFVSSPLRPLSTTSPPFSHSPEGSDAEPDPDPAPPADQGNQYRDQPRAPHTTRPLENGLDHGIYKAIMVGKVGQEPVQKRLRSGKTVVLFSLGTGGIRNNRRPLDNEEPHQYAERSSVQWHRVCIYPDRLGSLALKHVKTGSVLYLEGNLETKVFSDPITGLVRRIREIAVRGNGRFLFLGNDGNGPKIGEVKGVGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVC4</accession>
    <name>A0A8I6XVC4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVC4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0326000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0326000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0326000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0271910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0271910.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0271910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0326000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0326000.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0326000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0326000.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="172" mass="18502" checksum="E8024147FAF7E21B" modified="2022-05-25" version="1">MRRLVFAELVVLLAAALLLPVVICCRRWGGGVARPLPVSEQPGGSRKERRVERLRRALHALRAWCRRSRVEPAASASASPSPPPPDGAQAQREVQLEQVAAPAVEQAGPEEEGVAVWRERWFGGPEGSRALYTIEEASETEGDVESQCGGGAAEPETPFYTPPDSPAHGECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XWK3</accession>
    <name>A0A8I6XWK3_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">Carotenoid cleavage dioxygenase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123451037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983988.1">
        <property type="nucleotide sequence ID" value="XM_045128053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XWK3"/>
    <dbReference type="SMR" id="A0A8I6XWK3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0336120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0336120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0336120"/>
    </dbReference>
    <dbReference type="GeneID" id="123451037"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0280290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0280290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0280290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0336120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0336120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0336120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451037"/>
    <dbReference type="OMA" id="VMVKLMH"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF106">
        <property type="entry name" value="CAROTENOID CLEAVAGE DIOXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0336120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="62731" checksum="4C3639FAF2C9DF18" modified="2022-05-25" version="1">MGEAAAAMEDAQRKGLAVVVPAPRPRKGVASWAVDMLERLAVRLSHGKKAEPVPWLSGNFAPVPDETPPAAGLTVRGHLPKCLNGEFVRVGPNPKFTPIAGYHWFDGDGMIHAMRIKDGKATYISRYVKTSRLEQEEYFGGPKFTKIGDLKGVLGLFMVLTQELRKKLKVLDATYGIGTANTALIYHHGKLMALSESDKPYVIKILEDGDLQTLGLLDYDKRLKHPFTAHPKVDPFTDEMFTFGYSHEPPYCTYRVITKDGIMLDPVPITIPESVMMHDFAITENYSIFMDLPMFFRPKEMVKNSEFIYKFDPTKKARFGILQRYEKDEKNIRWFELPNCFIFHNANAWEEGDEVILITCRLNNLDLDQVNGNQSDKLEDPGNELYEMRFNMKTGDASQKQLSVSAIDFPRINESYTGRKQQYIYCTILDSTVKVSGILKMTGIIKFDLHAEPESSKEQLEVGGNVRGIYYLGPSRFCSEAVFVPKEPGVSHEEDDGYLIFFVHDENTGKSEVNVIDAKTMSGDPVAVIELPNRVPYGFHAFFVNEEQLGHQVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1R9</accession>
    <name>A0A8I6Y1R9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1R9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0455320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0455320.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0455320"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547850"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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    </dbReference>
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        <property type="entry name" value="Rx_N"/>
    </dbReference>
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        <property type="entry name" value="OS12G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="331"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="480" mass="54074" checksum="6CBF44D20E901D34" modified="2022-05-25" version="1">MDTAIGAAKWVLGKALAPVTDGLLEAWAASADLDRNIDALKMELLYAHGMLENAQGRGIRGVALKELLRKLEQLAYRADDALDELDYFRIQDALKGTYHATDMNAVGSVQGLRINVEHTARHVTTKLKCLPCLGAASRGGDQESDRGDHQENDGRRGISSSPPTPANKGVKKDGGRCMSKVISRARNTAHTFGKHFPCYSPISVHDDEPQAIMLGCASESDYFFTKGFTWASHEIACERDCDIEIPELIFDRVQMSTTMMDMVVQLKEVCAKVSIILNLDLLDSSRTRTQEIAINRSKTTSEIVEPEFYGRDDQKKKIVHVINSHEYSFSQLNVLPIFGTGGIGKTTFTQHICQEVNSYFQTSIWICVSLDFSADRLAQEIVNRIDRVDNEKQNATAEELIEQRLKAKRFLLVLDDLWTYREDEWKKLLAPFKKVESKGNFIIVTTRIPMVAEMIRTTDCQLELERLDDADTMHFFEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y366</accession>
    <name>A0A8I6Y366_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y366"/>
    <dbReference type="SMR" id="A0A8I6Y366"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0412980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0412980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0412980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044566">
        <property type="entry name" value="RMV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826:SF22">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826">
        <property type="entry name" value="POLYAMINE TRANSPORTER PUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0412980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="328" mass="34852" checksum="C98E9A17C91DF620" modified="2022-05-25" version="1">MSQEFLLVAGQPSPRQEQQVLHAVVREEERTPGDRQRQHGYAAVQQGRGPTLSGYGHKRNITLIPLVFLIYFEVAGGPYGAEKAVLAAGPLFTLLGFLVFPFAWGVPESLITAELATAFPGNGGFVLWAGHAFGPLAGFLLGLWKYLSVVINIAAYTALIADYLGGAVAQPGGARTGTVIGMTLLLSFVNYAGLSIVGWGAVALGMVSLAPFVLMTAMAVPKIRPSRWASQVTKGKKDWRLFFNTLFWNLNYWDCASTMAGEVERPERTFPRALAVAVVLIAGSYLLPLMAAIGATDASPDAWVNGYLADAQASSVAHGSNTGPGPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5X0</accession>
    <name>A0A8I6Y5X0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5X0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0351800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0351800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0351800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0351800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0351800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0351800"/>
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        <property type="component" value="Chromosome 4H"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0351800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17567" checksum="837EDC1873947304" modified="2022-05-25" version="1">MSWSSGHSAVAAPGFRRATRRREEESRSPVPYRESPMAYEPAKFCWCNPRRKAPRWISWSIPNPGRRYYACVDAMVSFILMVFLLFFPQFEDATPIVAVPEAQIDREVLAMSLQDQLKEKNAEIATLKRKYHNVVFVFAVFVLGLVAGKILLQ</sequence>
</entry>
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    <accession>A0A8I6Y6C0</accession>
    <name>A0A8I6Y6C0_HORVV</name>
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        <recommendedName>
            <fullName evidence="1">dynamin GTPase</fullName>
            <ecNumber evidence="1">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123404568</name>
    </gene>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044954441.1">
        <property type="nucleotide sequence ID" value="XM_045098506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6C0"/>
    <dbReference type="SMR" id="A0A8I6Y6C0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0616230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0616230.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0616230"/>
    </dbReference>
    <dbReference type="GeneID" id="123404568"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0511000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0511000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0511000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0616230.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0616230.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0616230"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404568"/>
    <dbReference type="OrthoDB" id="5562561at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Dynamin-2B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
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        <property type="entry name" value="Dynamin_N"/>
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    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DYNAMIN-2B"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="11">
        <location>
            <begin position="40"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="574"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="10">
        <location>
            <begin position="736"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="698"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="799"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="706"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="809"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="865"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="875"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0616230.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="913" mass="99507" checksum="7FFCC7DA73D92EAE" modified="2022-05-25" version="1">MSSSAMDAIVELVELSESMRQAASLLADDDPSDDAAPRRPSTFLNAVALGNVGSGKSAVLNSLIGHPVLPTGENGATRAPIVVDLQRDPGLSTKSIVLQIDSKSQQVSSSALRHSLQDRLSKATGPGRSRTDEIYLKLRTSTAPPLKLIDLPGIDQRVIDDSTINEFAGHNDAILIVVIPAMQAPEVASSRALKLARDIDPEGTRTIGVLSKIDQAASDAKTVACVQAILSNKGPRTAAEIEWVALIGQSVAIASAQSGSVGSENSLETAWRAEAESLKSILTTAPQNKLGRIALVDTIAKQIRKRMKVRLPNLLSGLQGKSQVVKDELARLGESMVQSPEGTRAVALELCREFEDKFLAHVTSGEGSGWKIVASFEGKFPDRIKQLPLDRHFDLNNVKRIVLEADGYQPYLISPEKGLKSLIKGVLEMAKEPSRLCVEEVHRVLLDIVNATANGTPGLGRYPPFKREIITIASNALDTFKSDAKKMVVALVDMERAFVPPQHFIRLVQRRMERQRREDEVKTKSSKRAQDAEQSMNKGSEQSGGSLKSSKDKSNQQDKDTKEGPNLQVAGPGGEITAGYLLKKSAKNNEWSKRWFVLNEKSGKLGYTKKQEERHFRGVIVLEECNLEEIEEEELSKTSKDSKKANGPEKGPSLLFKITNRVAYKTVLKAHSAVILKAESMADKIEWVKKIKGIIQSKGGSVKSSNTPEGGSMRQSHSDGSLDTMARRPADPEEELRWMSQEVRGYVEAVLNSLAANVPKAIVLCQVEKAKEDMLNQLYSSISSQSNARIEELLQEDHNAKRRREKAQKQSTLLSKLTRQLSVHDNRAAAASYSDDTSGPESGPQSPSQSGEDWRSAFSAAANGSADRSSSQNESRSRSADSRGRRYENGDANGANSGSRRTPNRLPPAPPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6J3</accession>
    <name>A0A8I6Y6J3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6J3"/>
    <dbReference type="SMR" id="A0A8I6Y6J3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0608710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0608710.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0608710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0608710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="53"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0608710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="16518" checksum="DC83BE3BDAF8C17A" modified="2022-05-25" version="1">MAYEPEVLCHCNPRRKAPRWISWSRANPGRRYYSCVNAMNGDRCGFVQWYDSPLPKFFSDLIGDLRDEVWRLRGQDNVPPVLVEQVEECRDQFVSVQPTVKDLQDQLKEKSAEITALKGNFEKVVFIVLVFVFGVVAGKLFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7A3</accession>
    <name>A0A8I6Y7A3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEFL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7A3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0523590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0523590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0523590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0435250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0435250.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0523590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0523590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0523590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035182225" evidence="5">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0523590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="81" mass="9015" checksum="3D675EAC8E9DC0FE" modified="2022-05-25" version="1">MRATQILLLVLVLFMLSPDLAKAKLECTDFLIANQCDVDEITCQHYCYNQFNGTGKCIHKKGCQCHFCYATPPKPSPASSS</sequence>
</entry>
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    <accession>A0A8I6Y7Q0</accession>
    <name>A0A8I6Y7Q0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123453124</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985824.1">
        <property type="nucleotide sequence ID" value="XM_045129889.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_045129891.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527810.1">
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    </dbReference>
    <dbReference type="GeneID" id="123453124"/>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000003">
        <property type="entry name" value="Trafficking particle complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sybindin"/>
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    </dbReference>
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        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0527810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="16215" checksum="B79D5E4FACB861C4" modified="2022-05-25" version="1">MGTVAIYSLFIINKSGGLIYYKDYGSAGRMDTNDSLRLASLWHSMHAISQQLSPTTGCTGIDLLQAHNFDLHCFQSLTGTKFFVVCETGAQNMEILLKTIYELYTEFVLKNPFYEMEMPIRCELFDLNLAQVIQKDRVALLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7S0</accession>
    <name>A0A8I6Y7S0_HORVV</name>
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        <recommendedName>
            <fullName evidence="5">Transmembrane protein 209</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123448927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981906.1">
        <property type="nucleotide sequence ID" value="XM_045125971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7S0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0385160.1">
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="entry name" value="Cytochrome_B561-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21780">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 209"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="94"/>
            <end position="113"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="228"/>
        </location>
    </feature>
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        <location>
            <begin position="272"/>
            <end position="314"/>
        </location>
    </feature>
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        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0385160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="71241" checksum="E302CEC0D3816DB7" modified="2022-05-25" version="1">MEFRHGGSPGGGGGDGKPREKFSVYQNPSLTRALASRSVRPSLPVLLLLALSPIASASSLMALSSREGQLFKVAGGADLSMAAVVFAVRLVEAALGLVALLTLPAFFRALMLYNGRKALAKEDKVVLSERQLGLLGLKTTGSEGSPMGEQTKKPPKAKPSTPSEPIVPIRRSSFSYTPSRVQPRIGSSHLSPGSERLTTLQMSPSTPLQKPVSSPSTPWSRKSSGSAKGIQTEAMLDQFLATLDENIDNVTDSACKTATPPATITGFGVASPVSVTTSTTPSGATRSTPLRPVRMSPGSHQKYSTPPKKGEGELPPPMSLEQAVDAFENLGVYPEIEQWRNNLRQWFSSVLMNPLVEKIKSSHVQVKQTTASIGASVNVSQVGSDLPSTTPPISLSPLGGTKDWQPTVTVDEDGILNQMRGALLQSRNAPVAQTFGSPQQPQSNLLLPAIQACIDAITEHQRLNALMKGELIKGLLPQSSVRADYTVHRVQELAEGTCLKNYDYMGYRDGYGKSEKKWTSELPTDSHLLLYLFAAFLEHPKWMLHVDPTSYSGAQSSKNPLFLGILPPKERFPEKYVALISGVPAIIHPGALVLAVGKQSPPVFALYWDKKLQFSLQGRTALWDAILLLCHQIKVGYGGIVRGTHIGSSALNILSVLDSDMES</sequence>
</entry>
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    <accession>A0A8I6YAI6</accession>
    <name>A0A8I6YAI6_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123399793</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
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                <person name="Langridge P."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044950111.1">
        <property type="nucleotide sequence ID" value="XM_045094176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAI6"/>
    <dbReference type="SMR" id="A0A8I6YAI6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0494470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0494470.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123399793"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0409850.1"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EMP24_GP25L"/>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
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    <feature type="chain" id="PRO_5035208255" description="GOLD domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="34"/>
            <end position="121"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        </source>
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        </source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6YAJ8_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
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                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAJ8"/>
    <dbReference type="SMR" id="A0A8I6YAJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0551820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0551820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0551820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0665160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0665160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0665160"/>
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        <property type="component" value="Chromosome 7H"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009635">
        <property type="term" value="P:response to herbicide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
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        <property type="entry name" value="GST_C"/>
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    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
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    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF85">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01154">
        <property type="entry name" value="Main.5:_Phi-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="90"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0665160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="225" mass="25089" checksum="D046A086BC66C699" modified="2022-05-25" version="1">MAPAVKVYGWAMSPFVARPLLCLEAAGVDYELVPMSREAGDHRQPDFLARNPFGQVPVLEDGDLTIFESRAIARHVLRKYKPELLVGDGSPESAAAVDVWLEVEAHQHHPVLVAITMQCLVAPLLGNATDQDVLDENLGKLRKVLEEYEARLSASKYLAGQSVSLADLSHFPMMRYFMETEFAALVEEHPHVKAWWEELKARPAARRVTEFMPPDFGLGKKAEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBE4</accession>
    <name>A0A8I6YBE4_HORVV</name>
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        <recommendedName>
            <fullName evidence="4">MtN19-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123397732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044948202.1">
        <property type="nucleotide sequence ID" value="XM_045092267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBE4"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0501750.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123397732"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0501750.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Stress_up-reg_Nod19"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298141" description="MtN19-like protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0501750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="46757" checksum="EC06BBB1937CBD38" modified="2022-05-25" version="1">MKRSMLQLGIVTLVVSSATAAFLPTTEAFNVRAPQLKTQTFLSPPFFLRPGGVVNKWYYDVDFPRGHVALKSFNGEVVDDAGAPVPLHETYLHHWLVAPYYVYGQSMIPITNSGPCKDSLGQHFGLGSETRQTATWVPDPYAIEIGKPAPAGYDERWFINVHAIDTRGAPDKLACAECRCDFYNVTVDEAGSRIGNGYVGGLHCCYDSTRCRVDGAFANNGEPPRKLFLRYTVSWLDWSDAVHVPVRIYILDVTDTALFDGNPNPYCKVEYRVDECSSEDRAKNNCVDMKTTEEMVSHGGDLVYAVAHLHRGGLGSSLHGQDGRLLCKSMPIYGTGQEAGNEEGYIVGMSTCYPEPGTVKVSHGEVLTMVSNYSSERQHTGVMGHFYILVADQQEQALNKPALCFSFPYSWCVPAWMWSNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YC14</accession>
    <name>A0A8I6YC14_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger-XS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YC14"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0704890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005381">
        <property type="entry name" value="Znf-XS_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03470">
        <property type="entry name" value="zf-XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger-XS" evidence="2">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="190" mass="20977" checksum="4184D2E0955FCE17" modified="2022-05-25" version="1">MEGARRAVALADLEVLKQQEDMTATVVAAAELEKEVPVPKQEDTTATAVAAADLEKEVPKQEDTTSSGAADDQEKEAVGMKRKLAKTGFKSPYDSDYDDDDLYEYNSGDDVEDGNKESFIEKEAQKIREKGKAAYFKRGMLRCPYCTIKPKPRDGIYEHLMLHAHGLSRSAEDIKTRAEHAALLKALGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YCS5</accession>
    <name>A0A8I6YCS5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0636120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0636120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0636120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="3">
        <location>
            <begin position="55"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0636120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="745" mass="82887" checksum="0B918FE9C91D83DE" modified="2022-05-25" version="1">MQLTATDAAVVIVKLVNDWVVRSLVLLSLATHLVLVLLAGVRRRRATGFGTALLWFSYQVAGWAATRSMSHLMLGIVTSSQQHLHAFWTPFLLMHIALPDNISAYSVEDNALSSRALALAPAQVAGAILALCKHFVVVHDAGALRPAAYLMFTLGIAKYIESVWALRQGNLGTIRRSIKKMKPDELSAEEHEGHGFKLNNEQALLLAHDLLYVSKGAFADYSFSLKPVEPDAAIQAVRAFSRSQGWEDMCKVVEMELSLMYDVMYTKAAVGHTWPGYCIRLASPLFTAAALLLFWSDNKDGQSSADVIVTYSLLIVTLVLDVGWLLRSLGSTWTCAFLKHSPYWLLGCSHRLIRSLGFMAVKYHLTSYRMWSSSGTIGQYNLLRESCRRRRGGTTMNISSRLVKMVATEDNWMEYQYGYSKGIALLPFVRDLLFQGIWEILKKTFQPPAQKVYAEVTAQVSVDPDPAGALGESPPPGPPPSAEAAKVEADLHAGQIAHSELGDALDIGPEFQEVVLRWHIATDIFLLCRPSSTTNSGRGSYKYVKVIQELSNYMMFLVAVRPGMLPGLYLLSMHDATREALDKFCCGLHAMPDHERTCCTCSPRDEMHFAEILKAMEPIEGSTSLILSEGRLYAKLMLSRVNANEMDPNDKTDIKKPIAQLKKLMPDLWWSEEDGVGDWSLPMQDMLEQHMLEHILGAWVRLLLHASTRCSRGSHAKQLGRGGDLTTVVWMLADHAALFRIPQHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YG51</accession>
    <name>A0A8I6YG51_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein POLAR LOCALIZATION DURING ASYMMETRIC DIVISION AND REDISTRIBUTION</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YG51"/>
    <dbReference type="SMR" id="A0A8I6YG51"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0575560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0575560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0575560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0693310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008356">
        <property type="term" value="P:asymmetric cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040348">
        <property type="entry name" value="POLAR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33476">
        <property type="entry name" value="EMB|CAB62613.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33476:SF16">
        <property type="entry name" value="OS06G0648500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36458" checksum="DF54458F1A053508" modified="2022-05-25" version="1">MAAEGTSTRRILDYLNDGEEFEFEGPSSAAAVTPRALAAARSLLPTFRWARLARLGRKGRADQGGARGGDRRRRGGGGHAQGRLCIVSQSNSVRRRTSHEQLLVPAACTVAVDDEATRLSGLSVGLSLVFLLAKTSDEFDKMARVQAEMEALIRDFKRQHAMATDNTGCEDDVPGDRNPDSAALSCLTDGNEPRAATGRCDDHRRCYNHQVTSSSGAEMEAASRRRMDVLKEEFHAELERVRAGYSADSLPFSIGEERGEGGAEPSDDDDDDIADCRNQERIEELEAALRRAEKRLFDKEMEASLWKDTAKMAFRHDHDHDDSRASPHLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGW2</accession>
    <name>A0A8I6YGW2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGW2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0481110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481110"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="349"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="353"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="622"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="645" mass="69028" checksum="22C63AE9B429AE6D" modified="2022-05-25" version="1">MEKESQPADHRLEPEEPEPVTSPGGAAAAPIYDVYLDEEEAYLEGLDVPAAPVYDADTEGAAAAAVEANWQVAMAADEAIAHAMHMQDLVEMDERDLGLYPASSGASVGFGARATAANFLESGTFDFESIHEVETGAGNSRIPGALESLPRPPTPSTVGVGAAASPARQTILSQDAGGSSSSQQVPGPGVAAAATVPSMTLPVRLAPRAGRCGAPYATAPRARPIGPADDPVSVKRRALLADAASWVMFPAMEEGHDNGWYEAIVGDGTEKLEQPEKQRLHESLQLCLPRSEPCNPAVAAAGEEEEKEEGGFSLPKFCQRWGVSPSDLEPDAPGPSMTKVPPLADHEVPTFDCGICMDTLPVFDLFHGLPCKHKFCATCMTTYVEGRIRASELPIPCPDAACKGKENGVLHPEMCKKAIDYGAFDDWGARLTESALPPDRRAYCPNRRCGVILETSGEAEPAMAPCPACKHLLCATCGMEWSPQGAGVEHDCAKGPDAALVKRLAQEHQWKRCPNCKMIVGRSFGCNRMTCRCGFVFCYQCGRPMSRGQPELAGLLEPCRCLDAGLAFAFAHHHQAHHVDLNVPPPHQPPLQQQEVAAMDALMNELPVWDEEPPVVFANRLEGDDGIHQPGPQDDPDVDMEIHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGW8</accession>
    <name>A0A8I6YGW8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">MDR-like ABC transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGW8"/>
    <dbReference type="SMR" id="A0A8I6YGW8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0700440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0700440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0700440"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0700440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0700440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0700440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000967">
        <property type="entry name" value="ABC multidrug transporter mdr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="630"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="204"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="512"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="832"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="444"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0700440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1084" mass="119055" checksum="88C4912C2CF6F13D" modified="2022-05-25" version="1">MGHFMSNFSTFLVAVIIAFVCCWEVGMMAFLVVPMLLVVGATYAKMMVGMSTTRIALVSETTSVVEQTISHIKTVFSFVGENSAMKSFIKCMDKQYKLGKKEAITKGLGLGMLQIATFCSYSLTIYIGALAVTRRSAKGGETIAAVINILSAAIYLSNAAPDLQTFSQAKAAGREVFKVIKRKPAINYESNGRILEKVTGHIEIREVDFTYPSRKDKLILQGFTLVIPAGKLVALVGSSGCGKSTVISLVQRFYDPISGDITIDGQNIKELDLKSLRRNIGSVSQEPALFSGTIMDNLRIGKLNATDEQIIEAAKTANVHSFISKLPDQYSTEVGERGVQLSGGQKQRIAIARAILKDPPILLLDEATSALDSESEKLVQDALDRAMQGRTVILIAHRMSTIINADKIVIVENGRVAQHGMHEELLKRSTFYSSVCSMQSLEKKSGKSEDRFTDQVEEEQDSATYKEQSFAAHEQEKKPEPTSKQLKQGIRKRTSAFNRIFLGTFKLVPGKVLVGSTAAAISGISRPLFAYYIMTVGMAYLDADTNKKVSKYSIILFLIGMLTFFSNIFQHYIYGLVGERAMNNLREALFSVILRSEIGWFEEPKNSVGFLTSRVVSDTSMVKTIISDRMAVMVQCISSIIIATAISTAVNWRMGLVTWAMMPCHFIAGLVQVRSAKGFATDNSKSHRKLISLTSEAVSNIRTVASFVQEEEILRKADLALQEPMRISRIESIKYGVVQGISLCLWHMTHAIALSYTIVLLERKLATFENCVRSYQAFAMTIPSITELWSLIPMIMSAIAILDPALDILDRETQIVPDEPKLNCEDRIVGNVEFEDVSFSYPSRPEVIILDGFSLAIESGQRVALVGPSGSGKSTVLALLLRFYNPCSGRVLIDGKDIRRYNLKSLRKQIGLVQQEPILFNLSIRQNISYGNEGASETEIVEAAMEANIHDFISSLSKGYDTVVGDKGSQLSGGQKQRIAVARTILKKPIILLLDEATSALDGESERVVMNTLGAKGWKSKGEFSSKITSITIAHRLSTVTNADVIVVMDKGEVVETGSHATLVSASNGVYSRLYNMQIKAGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YH83</accession>
    <name>A0A8I6YH83_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YH83"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0722210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="26597" checksum="774B214C7C681029" modified="2022-05-25" version="1">MLVRMMSVAGYRWYPVYTAEEEYRDFNGSQYLCTVRVFPDYPRAEEPIHWSYGLVVTVDMAVQDAAYSMLTIMRARHAQLQNSEFCYVPASQSGEEGYLSGVYFDFSMEDPLLQSNTEMLENRDRDARALRMERYATRAHPWTALTQLAPVVHTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPLVRGPRPHPRPYGSQGSQARVFSDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHJ6</accession>
    <name>A0A8I6YHJ6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHJ6"/>
    <dbReference type="SMR" id="A0A8I6YHJ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0599350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0599350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0599350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0722720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000020">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044521">
        <property type="entry name" value="AtbZIP8/43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46324">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46324:SF8">
        <property type="entry name" value="OCS ELEMENT-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="70"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722720.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="165" mass="17617" checksum="88EF78561039A11D" modified="2022-05-25" version="1">MQQELAALACQAGFVGHPTHHLPPHDVDFDLVDGWLASGSGGAGGGNGDGPPSCGSGLSHVAGAAAAVSETRKARRLASNRESARRSRMRRRRQLDELSACAAELRAANQRLVVDLNRAEARHAQVARENARLRGEVRRLRERLAAEEEATDRDATDEAAAARTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YHN9</accession>
    <name>A0A8I6YHN9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell division cycle protein 27 homolog B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123406944</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955897.1">
        <property type="nucleotide sequence ID" value="XM_045099962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YHN9"/>
    <dbReference type="SMR" id="A0A8I6YHN9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0723830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0723830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0723830"/>
    </dbReference>
    <dbReference type="GeneID" id="123406944"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0723830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0723830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0723830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406944"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000018">
        <property type="entry name" value="Cell division cycle protein 27 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="700"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0723830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="85685" checksum="8C07EB14A7909762" modified="2022-05-25" version="1">METLMVDRVHSSLRLFMNRNAVFLCERLCAQFPAETNVQLLATCYLHNNQPYAAYHILKGKKLPESRYLFAISCFRMNLLREAEETLCPVNEPNIEVPSGATGHYLLGLIYRYTDRVTAAAEQFTQALTLDPLLWAAYEELCILGVAEDANECFTEATALRLQQEQASTSNLEKSNFVNENRVLSPNVSASLVDISPKHIKQLSANNTAEVPGYPHLRATALHVQNSVPSNVLQFDTPSPTVTQTSGTAPPPLFRNLHAYQNTTSGDAPAKPKVNAVNQPPRRKYLDEARLKKVTGRLFNTSNDSVPRRSARLSRDTPINSNSNISQFGGNGPDHSSGKLRVNSSTSSKLCSTAIRSVQVRKGKPRATEIFDEGSRYEAVDEMWTDNIVATSSSLSIAEGRCFEQDKAERILSQDSKMATGIRELMGLLRTLGEGYRLSCLFKCQEALEIYRKLPEPQFNTGWVLCQVGKAYFELVDYLEADHFFELAHRLSPCTLEGMDIYSTVLYHLNEEMRLSYLAQELIFVDRLSPQAWCAVGNCFALRKDHETALKNFQRAVQLDSRVAYAHTLCGHEYSALEDYKNSVKFYRCALQVDERHYNAWYGLGVVYLRQEKFEFAEHHFRRAFQINPRSSVLMCYLGMALHALKRDEDALEMMEKAIFSDKKNPLPKYQKALILVGLQKYQEALDELERLKEIAPHESSMYALMGKIYKQLNILDKAVFCFGVALDLKPPAADLAIIKSAMEKVHLPDELMDDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKQ5</accession>
    <name>A0A8I6YKQ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKQ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0620630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0620630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0620630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0748200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0748200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0748200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF90">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="78"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0748200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="375" mass="41753" checksum="21408FD9D3E1AE6D" modified="2022-05-25" version="1">MGLSLGKITCSPWPDLPPDIVGLILSRLLCHTDRASFAAVCNGWRLAARQLSPSLPTAVPCITFGRSAYQSLTDDNVKVRHIAAPRGFRAGATFGSWVLYEHKLSGRCFLHNPSSPSTPPIEVPCRYLWSTSSHSGDVVVGDYEPCMAGPLKLEKMVVCSSDLVVAVFDKVFNFRYFLPETQPPTLLFWDAVVPMFGDEHRGRSFIKDITFHRGKVFTITQTEDLFAYDLVRDTPVLQTRVQHVIKEDPAADVPPVFEADMENKVWSEVKDLGGQVLFVGRSGSRALAAVDSHDDYNYNDPRFRGGNPVFILGNDWARVWSAVAECECSDCQKLVNSMPTHCVYDMASGKASLVSPKGHHSPMRSYRSEWFFPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMB9</accession>
    <name>A0A8I6YMB9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123398996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949368.1">
        <property type="nucleotide sequence ID" value="XM_045093433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMB9"/>
    <dbReference type="SMR" id="A0A8I6YMB9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421810"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421920"/>
    </dbReference>
    <dbReference type="GeneID" id="123398996"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0351090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0351090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0351090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0421920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0421920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0421920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398476"/>
    <dbReference type="KEGG" id="hvg:123398996"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="6"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="177"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0421920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="360" mass="38769" checksum="157799573573DAE0" modified="2022-05-25" version="1">MDWKPAVLMMAVVIMYAVLNALTKMAFNEGMHTTVFIVLRLLVAALFLSPIAYFKERKSRPKQTMEIFIYLFFSALLGASLIQWLFFLGLRYTTATFASAFNNTTPMFTFLLALAFKVEKIDVASRSGAAKLAGTAVGLAGAMVLALYHGPTLMGPPSGDHLAAAATHGGARRWAVGSVALVGFSASWSLWFILQSKIGTKYPPLYSSTAWMFVLSFVQMASVGAATEKMSLQVWVPRTALQAATVVFAGIGSSGLGFLAMSWCVERRGPVFTTAFMPLIQIVTAGIDVTVLHEQLHLGSVVGSAVVVVGLYLVLWGKSNEPSIDSKLPPPSHSKPECDDIKEDVCCSTALKEEKGEHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YS86</accession>
    <name>A0A8I6YS86_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953391.1">
        <property type="nucleotide sequence ID" value="XM_045097456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YS86"/>
    <dbReference type="SMR" id="A0A8I6YS86"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0577920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0577920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0577920"/>
    </dbReference>
    <dbReference type="GeneID" id="123403525"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0479190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0479190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0479190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0577920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0577920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0577920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403525"/>
    <dbReference type="OMA" id="GWCDQVQ"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1406">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0577920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="52060" checksum="7A885354B7E4164C" modified="2022-05-25" version="1">MGPQHFLVVAFPGQGHINPARALAERLSRAAPGARVTLSAAVSAHRRMFPSLASPDDEVHDGAISYIPYSDGFDHGFSLFAGDGDEVKRYAEVFGRVGRETFSAVVDRLAARGRPVTCVVYAMLMWWAAEVARERGVPRALYWIQPATMLAVYYHYFNGYERLVTEHAAEPGFTLSMPGLPPLAIRDLPSFFTNFTDGRIVAAFGDIRRTFQQLDLDVDGSSRTGGRQAMVLVNTVEELEAGALASVPELDVFPVGPAVVSLFAEGAGGASGTATAVGDLFEHDEKAYMEWLDTKPARSVVYVSFGSMSAVSKRQKDELKRGLAASGWPYLWVVRKNNRDDGFDDVGGVQGMVVGWCDQVQVLSHPAVGCFVTHCGWNSTLESVACGVSVVAVPQWSDQDTNARLVVQWGIGVRSTTDADRVLKAEELARCVEIIMGDTEEGAAIRASSASWKAKLQEAIADGGSSGRNLRTFLDQFPNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YX47</accession>
    <name>A0A8I6YX47_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YX47"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0630150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0630150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0630150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0523140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0523140.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0523140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0630150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0630150.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0630150"/>
    </dbReference>
    <dbReference type="OMA" id="NIVYLTC"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF37">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="86"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0630150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="238" mass="26329" checksum="0561832DD5991B50" modified="2022-05-25" version="1">MSEEMSMLGRARVAGDSSHPQHQEIPVAHCPQMWTDELLKVIKEANGMPVLFVPSSDGRLVAVALDRLQNLMPASREGSISDEMKEQNFRSWLLLLTSLVATVTFTAGLTPPGGFWSTDDDKDKKYRAGDPVMLNKSTYSPFYTSNTMAFFTSLMIIASLAKNRNSKKIMTIPFTCLVATCFICLGSSYISGTWISGSNPMLQLLPPVVVLGLNIVYMTAHRITEMWSSIIRKLRGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXF1</accession>
    <name>A0A8I6YXF1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123395092</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044945958.1">
        <property type="nucleotide sequence ID" value="XM_045090023.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945959.1">
        <property type="nucleotide sequence ID" value="XM_045090024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXF1"/>
    <dbReference type="SMR" id="A0A8I6YXF1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533590.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533590"/>
    </dbReference>
    <dbReference type="GeneID" id="123395092"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0443850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0443850.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0443850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0533590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0533590.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0533590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395092"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF69">
        <property type="entry name" value="PROTEIN BICAUDAL C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="191"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533590.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="26528" checksum="67DE9E11686C31C3" modified="2022-05-25" version="1">MADLHPLHPPEPDTNGGAAAASASASALLMPGDPAAEAAAAAAAAEPPAPPYSKRRRRPSVRLGDIGAQATASDAQLPRRHRKPSSHPRPPRRSHPDDALDPNARARPKPGQRRPRTAWIPAPHRGADGYEDEEGHHYYDDADQSDSAAAAAARAARVSGSREASVDESDGVADWGLPNGGAACYGGGGGVRAWLDGLGLSRYAPVFEIHEVDDEVLPLLTLEDLKDMGIGAVGSRRKMFAAIQKLRSSDTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z0Y7</accession>
    <name>A0A8I6Z0Y7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Branched-chain-amino-acid aminotransferase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123406340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955772.1">
        <property type="nucleotide sequence ID" value="XM_045099837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0Y7"/>
    <dbReference type="SMR" id="A0A8I6Z0Y7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0575220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0575220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0575220"/>
    </dbReference>
    <dbReference type="GeneID" id="123406340"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0476960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0476960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0476960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0575220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0575220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0575220"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123406340"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01558">
        <property type="entry name" value="D-AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000010">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000011">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001594">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19798">
        <property type="entry name" value="Sulfotransfer_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0575220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="62392" checksum="B0712F9AF560F6BB" modified="2022-05-25" version="1">MASPPEPAVIHAWSAPRSLSTSLMYSFSQRDDMDVLDEPLYANFLRVTGVDRPYRQELLSKMDPDGNKVVKEVIFGPGEKAYRYCKHIAKQRLPNLTGDLMKKGKHFILIRNPMNILPSFDKVVPPSFLELGVAELVAIYSELCDLGSPPPVIDADDLQRDPEAVLSGLCEDLGIPFQPQMLKWKAGPRDFDGIWAPWWYESVHTSTGFSKSRRYPMTFPFAFYDLLEQSLPFYNMLKRQVRRTAGSLLPPPPDPPLPVPRNKKILVWVGNELLPRDSARVSVFDSVVQGGDAVWEGLRIYDGKVFKLEEHLDRLFDSTKAMAFSNVPSRDWIKDAIFKTLNANGMFNDAHIRLTLTRGKKVTSGMSPAFNLYGCVLIVLAEWKPPVYDNSHGIKLVTATTRRNSPNSVDSKIHHNNLINNILAKIEGNLAQAEDAIMLDQDGFVSETNATNIFMVKKGIVLTPHADYCLPGITRATVMDLIVKENLVLHERRISLSEFHAADEVWTTGTMGEITPVVMIDGREIGDAKIGPVTRQIQNAYKVLTAGLGVPIPRNAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1B0</accession>
    <name>A0A8I6Z1B0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123408132</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1B0"/>
    <dbReference type="SMR" id="A0A8I6Z1B0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0651190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF764">
        <property type="entry name" value="AGAMOUS-LIKE MADS-BOX PROTEIN AGL62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="42197" checksum="E5728DA80BFDE8CD" modified="2022-05-25" version="1">MPRRGRRTGVAYVHDDKERDVTFFKRRRGLFKSASDLNVITGARVAVILETGNKKMHSFGTPSAGPIVDAFLSGAPLGNRLTDSETSAKIARLQSEVAQLDMEHGVEDKRNQLSIQHAKQIQEQYPGMVANLIFSKEQDINLEDAKKLFNELCRVHEDTRHRLPQLHHSYKAITGGVSVIQDMLPSSGPLAKSLKTIPSSGHSMQAYHLPQHHMPSAPIPSPPEHNVEPLFPQVPQMCHVASVASASQLALGLQAIPNQVQDLPPTDLHVEDYISPCDTVHPQQNYASPISTTEHNMASPMLVYSSSNDFVVDDPFGHDQWGFALPEQQSYNRFLEMDGYLDYNGTDVGQSTMGNGGWVDAPPESTSVKQDVDDRNKYGGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1Y9</accession>
    <name>A0A8I6Z1Y9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein SAUR32</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1Y9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0595760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0595760.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0595760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0493730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0493730.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0493730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0595760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0595760.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0595760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF57">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN SAUR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0595760.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15164" checksum="37CF63FE2A9AF685" modified="2022-05-25" version="1">MPRRRRVQEPRKIRVRSMKMHQEAAEVVTPAPIKKGCATFLVGGEVEDGASRRFTVPVELLVHPRIMELLGEAQEEYAYAYEGAIVLPCGVERFEKAVSAARAQERHRHQQHHHHHFSLAQLVGCFRPSHVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z201</accession>
    <name>A0A8I6Z201_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z201"/>
    <dbReference type="SMR" id="A0A8I6Z201"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0546340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0546340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0546340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0658330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0658330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0658330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0658330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="51217" checksum="3EC6B34EA1B98FB8" modified="2022-05-25" version="1">MDAHTGAPLTPLYSRDPPAPAAAAAAENPSAASVGAGAAAATGAPPTVGLARSLLLPPRMTTPTGGVAPAPSRAAAAPSKLPNASEPKKGKTSAKKKAADGSGSSKAKKKLAGRRTGAASTEAPMSSLVEPAADAHHVFDEMLPSLNDDAYMSTMGVGSNNSHWSQTNDIHLDDHEFEEDDASSDDGKRSPTPNSVSYSKPKRPDGCKKDKTEKKKRKGDDELTNAMEAIVKARKEANEVRKMTRNQDAAAEERRLAAEERRVAAEERKVALEERKVGMEERGKLLEWEKHLFFLDTSLFNDAQKEYVNLAREEVLIQKRAMIRGGLGGMGGGGLGAMGGMGLGAMGGLGGFGAMGGMGAPPATMGGMGGFGAPSNAMEGMGGFGAPSNAMGGMGGFGAPSNAMGGMGGMSFASLMGGMGVPPVAMGGMGAPPAMGGMSFDVPHTHTHENAVEELAKTVGATRDAVRDEVREEDSSSKAEESSAEEEEEEEEDEEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z6L1</accession>
    <name>A0A8I6Z6L1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123407021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044955985.1">
        <property type="nucleotide sequence ID" value="XM_045100050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z6L1"/>
    <dbReference type="SMR" id="A0A8I6Z6L1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0720010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0720010"/>
    </dbReference>
    <dbReference type="GeneID" id="123407021"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0597050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0597050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0597050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0720010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0720010.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0720010"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407021"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000214">
        <property type="entry name" value="EIN3-binding F-box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000451">
        <property type="entry name" value="EIN3-binding F-box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000473">
        <property type="entry name" value="EIN3-binding F-box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF173">
        <property type="entry name" value="OS06G0605900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="184"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0720010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="653" mass="68462" checksum="78E501E45EAB03CA" modified="2022-05-25" version="1">MPPFHDCRDGGLLVLDPAAAAAAMFGGVRHRKRARVTAVHPRVFAAAEEALRAAPAAKRQRLREAPTLDALPDGCLFEVLRRVQGARARGASSCVSRRWLALLGGIRASEIKRAQAPAVPDLNQVFVCEDEDQDGAEAASARPGRSERTLEGEAATDVALTAAAVADGLRGSLESVLVRGSHPTRGVTDSGISAVARGCPSLRSLALWDVPLVTDAGLAEIAAGCPSLEKLDITGCPLVTDKGLVAVAQGCPDLKTLTIESCSGVANEGLKAVGRCCSKLQAVNIKNCAYVGDQGVSGLICSATASLAKVCLQGLSITDASLAVIGYYGKAITNLTLTRLSSVGERGFWVMANALGLQKLRCMSITSCPGVTELALVSIAKFCPSLKQLYLRKCSQISDGLLKDFAEAAKVLENLQIEECNRVTLTGILSFLLNCSPKFKALSLVKCTGIKDICSAPAQLPVCKSLRSLTIKQCPGFTDSSLAVVGMICPHLENLDLSGLGAVTDDGLLPLIRSSESGLVHVDLNGCENLTDAAISALVKAHGTSLAHLSLEGCSKITDASLFAISESCSELAELDLSNCMVSDYGVAVLASAERLKLRVLSLSGCLKVTPKSVPFLGSMPASLEGLNLQFNFIGNHNIASLEKQLWWCDILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z7P2</accession>
    <name>A0A8I6Z7P2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z7P2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24611" checksum="E702453240A4CBA7" modified="2022-05-25" version="1">MRYKEMAASKGKPFPFKHAWAILQTFDKWKLRDQETAPKKSAMLRMDDSEDEEKERNLGKPEGTKKGKQRVKMEGEASSLREKMEQMMKAREELTRKTLETNILITEKKKEVKLAQVEAKREEAKRKADLEERMIKVKEAKVWKELMVEEKEHMMMSKKDMDEEQLQWWKDYKEDIAERKRIFRGASSTFRGDTPMSGGGDGGVDDSNGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1H4</accession>
    <name>A0A8I7B1H4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor TFIIB cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1H4"/>
    <dbReference type="SMR" id="A0A8I7B1H4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0064530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0064530.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0064530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0079210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0079210.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0079210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF67">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIB CYCLIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor TFIIB cyclin-like" evidence="4">
        <location>
            <begin position="126"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0079210.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="328" mass="34297" checksum="A66B2D202572FE9E" modified="2022-05-25" version="1">MELPRTRGSGGRQPPLYAPHSHPMEPSLVAAARAAHPDGGRVTAAAVVGCICLDHLACTECDRILGIPFLPIDARSPSPRRASTPGLASGSGSPPVPMARVSVGLRLGIGPSTGGDGARAEGFGASISGMAERLGLAAAVGDRAEEVFRKMEEARAWPRGPGWAKGRSRRDSGRLYAACLSIACRNEGSPRSLRELAMATADGSAAARKEIAKLTAHIRKRLGEEEAGQATGVGAVPVSGYLRRFGPLLGLGDREAAAAWEAARRLEGGALEVRHNAEALAAAVVCMALERAGARRHIREVATATGIPYATIYLVCRKMRPHTALLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B605</accession>
    <name>A0A8I7B605_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123444722</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044977475.1">
        <property type="nucleotide sequence ID" value="XM_045121540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B605"/>
    <dbReference type="SMR" id="A0A8I7B605"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0297570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0297570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0297570"/>
    </dbReference>
    <dbReference type="GeneID" id="123444722"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0297570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0297570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0297570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444722"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10304">
        <property type="entry name" value="GST_C_Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000047">
        <property type="entry name" value="tyrosine--tRNA ligase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF45">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="8">
        <location>
            <begin position="255"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="156"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0297570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="416" mass="44805" checksum="E96EE589590CBEAD" modified="2022-05-25" version="1">MAAAADFTQSKQAMSYALCKHLNLDPNSISSTLIKENDIVSLFSHIVTSPQDEVLFLHVKKWVDFSSNFAVSDGEQHAVLGNLNQHLSQMSVLLGGFKPSAADIIVFATVHVFMCHLSDSELQKYPNILRWMDYIQNVVDFGMTLQKINLTKSIFNPPAPAPSHPKKADKGDGDPNSKKAVSGQKNADKSDGAADSKKAAGENKVPGDKANPTSAKNNKPSGDKKKAQEKTAGKTTEAAAEKAPQKSAEKDSECSVSILNIQVGVIRKAWKHPSADSLLVEEIDLGDGNVRQVVSGLAKFFSPDELVNRHVVLITNVKPGKLRDVMSAGLVLCASNKDHTVVEPLIPPEGAKLGERISFAGFDGKPEDVLNPKKKQLDKITPHLRTDENGIATFRGVPFTTSAGPCRSSVPNADVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6S5</accession>
    <name>A0A8I7B6S5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123447647</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044980197.1">
        <property type="nucleotide sequence ID" value="XM_045124262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6S5"/>
    <dbReference type="SMR" id="A0A8I7B6S5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339290"/>
    </dbReference>
    <dbReference type="GeneID" id="123447647"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0283040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0283040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0283040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0339290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0339290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0339290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447647"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="110"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="328"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="601"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0339290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="657" mass="72797" checksum="1669F87F70EDAD92" modified="2022-05-25" version="1">MAKKQRQRGPPMGEEHVEAEAAAHEDAPAAAAEEEAAAREAGGAGREVAAGRSADAANGGVKEEGKEAAAAAEEEEREVTFDELGLDEQLKRALRKKGLTKTTPIQREAIPLILEGKDVVAKAKTGSGKTFAYLLPLLQELLKLCKEGRIRKSASNALILVPTRELCQQVYNEASSLLELCTSKLKIVQVTASMSDKDIKLALSGPPNILVTTPACVAACISKGIVQGSSIKESLSMMILDEADLLLSYRCEDDLKALVPHIPRSCQSILMSATSSPDIDKLTKLLLHNPFVLTLTEVGHAKDDVVPKNVQQFWISCSAKDKMLYILSLLKLELIQKKVLIFVNSIDNAFRLRLFLEKFAIRSAVLNAELPQNSRLHIIEAFNARLFDYLIATDDNKTKDAKQTNKESKKDSKASRKHLQQTLDAEFGVVRGIDFKNVFTVVNFDMPPNPAGYIHRIGRTGRANKTGASISLVSPEEENVFEEIEHMLQDVEKKDTNCILPFPLLTKNAVESLRYRAQDVARSVTSRDIQEARRQDVKNEILNSEKLKAHFEENPTDLDLLKHDKLLTNKSIPAHLRDVPEYLIDPTTKEASNAVKLSRAAMGLDNPGRKKRMGFRSGSGKSSDPLRTFSAEGKSRRRRGKEREGDDRRKRRKKAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7Y8</accession>
    <name>A0A8I7B7Y8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)H dehydrogenase subunit H</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7Y8"/>
    <dbReference type="SMR" id="A0A8I7B7Y8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0423390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005649">
        <property type="entry name" value="PRK07415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="9">
        <location>
            <begin position="124"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="393" mass="45603" checksum="48C42250E8CA2F07" modified="2022-05-25" version="1">MSLPLTKKDLMIVNMGPQHPSMHGVLRLIVTLDGEDVIDCEPILGYLHRGMEKIAENRTIIQYLPYVTRWVYLATMFTEAITVNAPEFLENIQIPQRASYIRVIMLELSRIASHLLWLGPFMVDLGAQTPFFYIFRERELIYDLFEAATGMRMMHNYFCIGGVAADLPYGWIDKCLDFCDYFLRGVVEYQQLITQNPIFLERVEGVGFISGEEAVNWGLSGPMLRASGIQWDLRKVDPYESYNQFDWKVQWQKEGDSLARYLVRVGEMSESIKIIQQAIEKIPGGPYENLEVRRFKKEKNSEWNDFEYKFLGKKPSPNFELSRQELYVRVEAPKGELGIYLVGDDSLFPWRWKIRPPGFINLQILPQLVKKMKLADIMTILGSIDIIMGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B958</accession>
    <name>A0A8I7B958_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123395583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044946537.1">
        <property type="nucleotide sequence ID" value="XM_045090602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B958"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0482940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0482940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0482940"/>
    </dbReference>
    <dbReference type="GeneID" id="123395583"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0400350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0400350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0400350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0482940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0482940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0482940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395583"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487:SF17">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487">
        <property type="entry name" value="OS06G0651300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="643"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="778"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0482940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="808" mass="85603" checksum="52522C61E4C69220" modified="2022-05-25" version="1">MEFPRRGPATGDGRRFPDPPPHGEEEPMTVMRNALLSQLHMDHLRQEIIVSELAKMERANALRAGVATADAGQSKPCPFSAEQLMALRAATPIPLLGLAAADAGRYGLLPFSAEQPMALRASACEQQTTRMPSQSVVAADAGQSTTCPFSAEQPMALRASACSQQDTRMPLHSVVAADAGQSMPCPFSAEQLMALHAAPSSHQDTQMALHGVAAADAGPSKLWPLSVEQLMALRAAADSHHATPMPMYGEAATDAGRSKPGPFSAEQPMSLLAAAGSHQNMPMPMYGVVAADAGRSKPWSFSAEQPMSLLAAAGSHHNMPMPMYGAAAADAGRSKPWSFSAEQPMPLLAVAGSHENTPMSLHGVAAADAGPSKLSPFSVQQLMALCAPAGGNQATPMPLHGANAADAGWSKPLPFSAEQPMTVHAAAAGHQVIPIPLHGAAAADAGRSKPCPSSADQFMSQQNAKFDEHKPPDSNKAVPQSKTSLAEKLELTGINIPVKKPKPLMKWNCTVCQVQATCEKNLQMHYAGQKHLANVAMLDPVTKPTDQKAKAAAAEPSLGTEQKKTSSINWSCSTCQARGTSKSTFDAHLQGKRHQQNIAEASVKGDGDGDGNGAPKNATAGEAKSDGVTMPNHSKKPSSVWSCSICQTTCTCETDLKNHLSGTRHREKVQSLLEESKNMARNPETNLKKNNAPQMVKNKGPHPAWNCIMCEAKCYSKSQFENHCSGSRHQQKIEAIIGKCETAKPSSMRTANEPSSDGSSGRNASSEKAEKKATLYFCEVCNLLCSSSETLLSHRYGKKHREKLSAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAN8</accession>
    <name>A0A8I7BAN8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MIZU-KUSSEI 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123397468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044947929.1">
        <property type="nucleotide sequence ID" value="XM_045091994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAN8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537850"/>
    </dbReference>
    <dbReference type="GeneID" id="123397468"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0447550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0447550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0447550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0537850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0537850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0537850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397468"/>
    <dbReference type="OrthoDB" id="672310at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010274">
        <property type="term" value="P:hydrotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006460">
        <property type="entry name" value="MIZ1-like_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01570">
        <property type="entry name" value="A_thal_3588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31696:SF4">
        <property type="entry name" value="OS08G0171800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31696">
        <property type="entry name" value="PROTEIN MIZU-KUSSEI 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04759">
        <property type="entry name" value="DUF617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0537850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="30211" checksum="017AF18ADB1E8B12" modified="2022-05-25" version="1">MGFPNVVDGPQSRTSSSVASSGTTRTSTDDAGNAPAGHTLEAPSRKTRSGRISPAQLFQKLRRALPILAPRCGKTARESETAAASTSQSQSQSHLMSRHVASGGGRRPCRRVTGTLFGRRKGRVALALQETPRSLPSLVVELALQTHALLRELGNPAGARIVLETERRRGAGEGPSKRAPLLEEAAWTMFCNGRKTGYAVRREATDYDLTVMETLRAVSMGAGVLPVPVPAGAAAGGGGGDDEVAYMRGCFEHLVGSWDSESLYMVGPQQGGGTGTGPELAVFFVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAT8</accession>
    <name>A0A8I7BAT8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KANL2-like probable zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BAT8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0450030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0450030.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0450030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0541120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0541120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0541120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026316">
        <property type="entry name" value="NSL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025927">
        <property type="entry name" value="Znf_KANL2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13453">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13453:SF9">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13891">
        <property type="entry name" value="zf-C3HC3H_KANSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANL2-like probable zinc-finger" evidence="3">
        <location>
            <begin position="66"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0541120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="197" mass="22292" checksum="4BB6BF0D6F430643" modified="2022-05-25" version="1">MEAIAEALTREEVLRRRRRRAARLLAAYRRLYWAMAEEVRSLHRQYVWELGCSPLEAEQPKRKKCGVTGCKVRAMAMAKYCHYHILSDPDQVLYKGCGHMMIKSGAQAGKNTHNVPILKASVPSLCNVHLQRSQRNISQAYKTVGFNPPPTGQISPNFSVLVAECVRKIQAQRREYPSAAAGKKYPDSDGKVRWAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BB00</accession>
    <name>A0A8I7BB00_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PI31 proteasome regulator N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123402562</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome inhibitor PI31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044952419.1">
        <property type="nucleotide sequence ID" value="XM_045096484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BB00"/>
    <dbReference type="SMR" id="A0A8I7BB00"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547320"/>
    </dbReference>
    <dbReference type="GeneID" id="123402562"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0454870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0454870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0454870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0547320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0547320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0547320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402562"/>
    <dbReference type="OrthoDB" id="68090at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045128">
        <property type="entry name" value="PI31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021625">
        <property type="entry name" value="PI31_Prot_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266">
        <property type="entry name" value="PROTEASOME INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266:SF1">
        <property type="entry name" value="PROTEASOME INHIBITOR PI31 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11566">
        <property type="entry name" value="PI31_Prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator N-terminal" evidence="4">
        <location>
            <begin position="18"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0547320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="313" mass="34161" checksum="3DB352BF1BED71D4" modified="2022-05-25" version="1">MVTAEAVMAVVRAARPVFCCAHDGVAFAANAAFLADGYSLCAVGPAALINPRPAEEEEVGIDGWNSMDNIYAFLYYKEDDGKKKFILMKCVVIADFLAIDALDLEAQHEGRFIVQINVKDFYSEEQHKNYKDMYKNFVCLINILNTTLLVELGRKDTTAVQIPDVENSSSMSSSGNFTWENPSTRTTEPAGSQYILWESRSTRMIEPAGLIYPPVVLVGDDDTFPVPGVGLYPHSGETGGSMHVGPNDPRFFPTNPSTPFGDLGSVPPGGRYDPIGPPDASGFEPSRFVSPSRHSGGSTHPDLEFFQQGPDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BDA2</accession>
    <name>A0A8I7BDA2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDA2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0652310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0652310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0652310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0652310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0652310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0652310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0652310.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25924" checksum="10E60428FE00ACCB" modified="2022-05-25" version="1">MSVCGYRWYPVYTVEEQYRDFNQSQYICTVRVFPDYPRAEEPIHWSYGLGVTVDMAVQDATYSMLTIMRARHALLQNSEFCYVSASQSGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDTRALRMELYATRARLWTALTQLAPVVQTGYGDMDMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGX8</accession>
    <name>A0A8I7BGX8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGX8"/>
    <dbReference type="SMR" id="A0A8I7BGX8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0582320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0582320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0582320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF99">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="235"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0582320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="415" mass="47168" checksum="151A372F33BC0F5D" modified="2022-05-25" version="1">MPRGRTAHSRFKIPLSCDDGASCSFAKQSGTAKLLRMALLILWDEATMTKRRVVEALDNSMHDIMGRRDRPFGGKTVVFGVDFRQVLPVVRRGSRGQIIDATLRSSHLWKGMRQLRLVTNMRAHNDTWFADYLLRVGNGIEEADDHGNIQLPQDICVPSTGEMNDIEKLIDHVFPGLDENMFDPNYMTCRAILSTTNDNVDNINLRMINRFKGEEVIYHSFDSAEDDPYGYYAPEFLNGLTPNGLPPHALKLKLNCHVILLRNIDPANGLCNGTRLVVRGFERNAIDAEIMIGQHAGRRVFLPRIPLCPSDNDMFPFKFKRKQFPLRLSFAMTINKAQGQTIPIVGVYLPNPVFSHGQLYVALSRATTKRNIKILIEKEKDKGKKQTNKSNKRKRPTLCLQRTMKNIVYKEVLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJT1</accession>
    <name>A0A8I7BJT1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJT1"/>
    <dbReference type="SMR" id="A0A8I7BJT1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0728680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0728680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0728680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0604470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0604470.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0604470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0728680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0728680.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0728680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="5">
        <location>
            <begin position="70"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0728680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="266" mass="30271" checksum="13F77D0CBA1AAB5C" modified="2022-05-25" version="1">MARLQSDAMEISDNCPSEAVSTNTHTENIVATLPSREGWSTPLVLYNNCWIRPHSLKDAMAVQDKLKARPDDIILAAHPKCGTTWLKALLFTVLNRSRYTFADHPLLTVRPHQLVPFIALRPGDLDRAEMLPSPRLLSSHLPLPLLPPAVFTLGCRIVCVCREPKDAFLSRWHFEKKLNQGMSLGMDEAFKMFSEGCSPYGPFWDRYLEYWKESLARPRQVMFLRYEEIMLDTPKVIRKLASFLGVPFTQEEEGGRVVKHKSGSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYR9</accession>
    <name>F2CYR9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123445549</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="AK356775">
        <property type="protein sequence ID" value="BAJ87990.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK357657">
        <property type="protein sequence ID" value="BAJ88871.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366578">
        <property type="protein sequence ID" value="BAJ97781.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978485.1">
        <property type="nucleotide sequence ID" value="XM_045122550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYR9"/>
    <dbReference type="SMR" id="F2CYR9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073560"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073560.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073560.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073560"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073560.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073560.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073560"/>
    </dbReference>
    <dbReference type="GeneID" id="123445549"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073560.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073560.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073560.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073560.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445549"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CYR9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000028">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF412">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP20-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="32"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="148"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ87990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ97781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="186" mass="19873" checksum="359383EA8788B86C" modified="2011-05-31" version="1">MAETIDLTGDGGVLKTVVRKAKDDAISPSDSLPLVDVHYEGTLAENGEVFDTTHEDNSIFSFEVGQGAVIKAWDLALRTMKVGEVAKITCKPEYAYGSAGSPPEIPANATLIFEVELVACKPRKGSSLGSVSDEKARLEELKKQRELAAATKEEEKKKREEAKAAAAARVQAKLDAKKGKGKGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2D1T7</accession>
    <name>F2D1T7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123411900</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot</tissue>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK357844">
        <property type="protein sequence ID" value="BAJ89058.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372098">
        <property type="protein sequence ID" value="BAK03296.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960793.1">
        <property type="nucleotide sequence ID" value="XM_045104858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D1T7"/>
    <dbReference type="SMR" id="F2D1T7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715200"/>
    </dbReference>
    <dbReference type="GeneID" id="123411900"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0593230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0593230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0593230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0715200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0715200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0715200"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411900"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052992">
        <property type="entry name" value="SDR_member_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44656">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44656:SF7">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ89058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK03296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0715200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="324" mass="35609" checksum="07F16B43B93A4915" modified="2011-05-31" version="1">MFIQKAWRTAAFGLYGFTQFTKSGFVEHAKKFREEDMQIRLDGKNCLVTGANSGLGYATAEGLASHGATVYMLCRNKERGETALNEIRSKTGNMNVHLEICDLSSINEVKSFATKFSSSEKPLHVLVNNAGLLEHKRTTTPEGLELNFAVNVAATYTLTELVMPLLEKASPDARVITVASGGMYTEPLNTNLQYSESEFDGTKQYARNKRVQVALTEWWAQKYSNKGVGFYSMHPGWADTPGVSKSLPGLSEKLSGNLRSNEEGADTVVWLALQPKEKLVPGAFYFDRAEAQKHLKFAGTAASHEQIGSIVDSIRSICNLPANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2D3A1</accession>
    <name>F2D3A1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aldehyde oxidase</fullName>
            <ecNumber evidence="3">1.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC123452610</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an aldehyde + O2 + H2O = a carboxylate + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16829"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="EC" id="1.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="20">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="20">Binds 2 [2Fe-2S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Aldehyde oxidases (AO) are homodimers and heterodimers of AO subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.3.1" evidence="3"/>
    <dbReference type="EMBL" id="AK358358">
        <property type="protein sequence ID" value="BAJ89572.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985228.1">
        <property type="nucleotide sequence ID" value="XM_045129293.1"/>
    </dbReference>
    <dbReference type="SMR" id="F2D3A1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517290.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517290.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517290"/>
    </dbReference>
    <dbReference type="GeneID" id="123452610"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0429860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0429860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0429860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517290.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517290.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517290"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452610"/>
    <dbReference type="OrthoDB" id="8300278at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004031">
        <property type="term" value="F:aldehyde oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009688">
        <property type="term" value="P:abscisic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009851">
        <property type="term" value="P:auxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000006">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000003">
        <property type="entry name" value="Aldehyde oxidase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000012">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
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        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
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        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
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    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
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        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
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        <property type="entry name" value="FAD-bd_PCMH"/>
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        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
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        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
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    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF160">
        <property type="entry name" value="ALDEHYDE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CO_deh_flav_C"/>
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    </dbReference>
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        <property type="entry name" value="FAD_binding_5"/>
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        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MoCoBD_1"/>
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    </dbReference>
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        <property type="entry name" value="MoCoBD_2"/>
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        <property type="entry name" value="Xanthine_DH"/>
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    </dbReference>
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        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 20" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9" id="KW-0937">Abscisic acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0073">Auxin biosynthesis</keyword>
    <keyword evidence="8 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 20" id="KW-0408">Iron</keyword>
    <keyword evidence="12 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 20" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="21">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="22">
        <location>
            <begin position="240"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="1279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
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            <label>1</label>
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        <location>
            <position position="60"/>
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            <name>[2Fe-2S] cluster</name>
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            <label>1</label>
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        <location>
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            <name>[2Fe-2S] cluster</name>
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        <location>
            <position position="122"/>
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            <name>[2Fe-2S] cluster</name>
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        <location>
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        <location>
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            <name>[2Fe-2S] cluster</name>
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            <label>2</label>
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            <name>[2Fe-2S] cluster</name>
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            <label>2</label>
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        <location>
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        <ligand>
            <name>FAD</name>
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            <position position="785"/>
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        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
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        <ligandPart>
            <name>Mo</name>
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        </ligandPart>
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            <name>Mo</name>
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            <dbReference type="PIRSR" id="PIRSR000127-3"/>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2D576</accession>
    <name>F2D576_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123399333</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK359036">
        <property type="protein sequence ID" value="BAJ90247.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949683.1">
        <property type="nucleotide sequence ID" value="XM_045093748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D576"/>
    <dbReference type="SMR" id="F2D576"/>
    <dbReference type="STRING" id="112509.F2D576"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0420980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0420980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0420980"/>
    </dbReference>
    <dbReference type="GeneID" id="123399333"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0350450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0350450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0350450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0420980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0420980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0420980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399333"/>
    <dbReference type="OMA" id="IELMQPF"/>
    <dbReference type="OrthoDB" id="624830at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D576">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF90">
        <property type="entry name" value="CSC1-LIKE PROTEIN RXW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="539"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="5"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="186"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="10">
        <location>
            <begin position="346"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="693"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ90247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0420980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="767" mass="86915" checksum="8C1CA9DB333343E2" modified="2011-05-31" version="1">MKISALMTSAGINIGLCVLFWSLYSVLRKQPAFVRVYFGRRIAEENRLLREAFILERFVPSTGWIVKALQCTEEDLLAAAGLDAVAFNRMLVFSIRIFSLAAILCLFGILPVHYLARKTQHLEIPSEQLHMFTVQNVEVQSRWLWVHSVVLYIISGVACFLLYVEYGHIARLRLLHLKRTTLNPGQFTVLVRGIPKTANESCSSDVDDFFTKYHASSYLFHQVIYKAGKVQKIMTGAKKACGKLDHSTSTDTTLDQSRKAITYPCCLCGASSNSFQLLPTDEVAKNIDNEECAAAFVFFKTRYGALLASQALQTSNPTKWVTDLAPEPDDMYWSNIWLPYKQLWIRRIATLLGSLVFSFLFLIPVTFIQGLSQLDQVHRKLPFLNGLLKQPYMSQIITGYLPSVILLLFLYTVSPIMILFSTLEGPTSHSERKKSACSKVLYFFIWNVFFVNLTSGAVITQLNSSSTTKDIAVQLAGVIPGQTTFFITYVLTSGWASLSSELMQLFGLIYNFIIKYVLRMKEDTAFVPTFPYHTEVPKVMLFGLLGFSCSVLAPLILPFLLVYFFLGYVVYRNQLLNVYRMRYDSGGLYWPIAHNTVIFSLVLTQIICLGVFGLKKSPVAAGFAIPPIFFILSFNQYCRTRFLPLFKTFPTQDLIDLDREDVRSGRMEHIHHGLRSAYRQFPDTEDIKMEKILTVGNDEDQDEGRSSSEPKGEETCEEQEPTRDLSHPTLKGLPVSRLQNSVRSITVICTTEKRSDNTALAHRLTNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDH6</accession>
    <name>F2DDH6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123442113</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK361943">
        <property type="protein sequence ID" value="BAJ93147.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974149.1">
        <property type="nucleotide sequence ID" value="XM_045118214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDH6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0064080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0064080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0064080"/>
    </dbReference>
    <dbReference type="GeneID" id="123442113"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0051060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0051060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0051060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0064080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0064080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0064080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442113"/>
    <dbReference type="HOGENOM" id="CLU_011027_1_1_1"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043303147" evidence="1">
        <location>
            <begin position="21"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="58"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ93147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0064080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="621" mass="67866" checksum="5F0E8F7B32F3F75C" modified="2011-05-31" version="1">MPPLALLLLPLLHLLSPALAEAPDRYAVRYAPPGANGNAAAQEYLDPTYPLPGPPPGAPACTVPVLSYSFANTYGAPPAKAAYAPPPECPAPWSQVVLSFSAACAGDQYDRVAAVWLDGSELLRTTTAEPTPEGVSWTVRKEVTRYSALLRSPPAGVLSVMLENLVNDQYTGVYNVTVSLEFHGIPAYLSQVNPPSSDAGVAHPSPATPTLPDSYFQPADLVLPISEATGDGGYWFRIQNSSDSRSKLVTIPSSTYRAVLEVFVSPHSNDEFWYSNPPDLYIQENNLTTPRGNAAYREVVVSVDHHFAGSFVPFPVIYTGGINPLYWQPVSALGAFDLPTYDIELTPFLGLLVDGKAHEFGISVVDGIAEWLVDANLHLWLDPSASAVQASLGRYRTPRLSISRRYSTQLLNGSFSIRAKRKSFFSGWVKSSLGNLTTEVETELETRSLVEFTNDGRNKTVQMKAEQETEVLVRSEARKVIGKLKTESEYPLSFYMDTEDGEDGTSLITGSLSHTLSLETKVKSDRFENEAELVDEQTAEGWMVVKDHDVINGSAATSQMYRYSDDRWRYRRMIDAVDGLVLGDNVSESYRVQGAAKACLPGRRCDGTASAAERWVDIAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F2DEK2</accession>
    <name>F2DEK2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby-like F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123402947</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="AK362319">
        <property type="protein sequence ID" value="BAJ93523.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952851.1">
        <property type="nucleotide sequence ID" value="XM_045096916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DEK2"/>
    <dbReference type="SMR" id="F2DEK2"/>
    <dbReference type="STRING" id="112509.F2DEK2"/>
    <dbReference type="PaxDb" id="4513-MLOC_57271.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607550"/>
    </dbReference>
    <dbReference type="GeneID" id="123402947"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0503680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0503680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0503680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0607550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607550"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028236_3_0_1"/>
    <dbReference type="OMA" id="TWPARNV"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DEK2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd22153">
        <property type="entry name" value="F-box_AtTLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF104">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="66"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby C-terminal" evidence="4">
        <location>
            <begin position="127"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ93523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="427" mass="47291" checksum="4838621095A05875" modified="2011-05-31" version="1">MSLKSIVRELKEMRDGIGSMSRRGGVSDGRAGHGRGGSRHSWPSLWPEPQPQPQRPGQEPQQQGQWANLPPELLIDVIQRVESSEVAWPARRYVVACAAVCRTWREVTKDVVKTLEECSRITFPISLKQPGPRDCPVQCFVRRDRATSTYLLYLGLSPSLHGENDKLLLAARKIRRAARTSFVISLISDDFSHSSSTYVGKLKPNFLGTKFTIFDSQPPCDAAVLPNNKPSKRHSKQVSPRLPLGNYNVATVSYELTVLRNRGPRRMQCTMHSIPAVCIQEGGKAPTPTGMVHSLDEQVSTLSTSSKGKEPNIEFSSTSLTADLSGPISTSEAPLLLKNKAPRWHEQLQCWCLNFRGRVTVASVKNFQLVASVDPSLGVPAAEQEKVILQFGKIGKDIFTMDYRYPLSAFQAFAICLTSFDTKPACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2DN93</accession>
    <name>F2DN93_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123411643</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="EMBL" id="AK365361">
        <property type="protein sequence ID" value="BAJ96564.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960539.1">
        <property type="nucleotide sequence ID" value="XM_045104604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DN93"/>
    <dbReference type="SMR" id="F2DN93"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718990"/>
    </dbReference>
    <dbReference type="GeneID" id="123411643"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0596290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0596290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0596290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0718990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0718990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0718990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411643"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF276">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044524093" description="Peroxidase" evidence="18">
        <location>
            <begin position="26"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="27"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="240"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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        <location>
            <position position="243"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
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        <location>
            <position position="248"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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        <location>
            <position position="64"/>
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    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="37"/>
            <end position="117"/>
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    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="70"/>
            <end position="75"/>
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            <end position="316"/>
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    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
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            <dbReference type="ARBA" id="ARBA00022617"/>
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            <dbReference type="PIRSR" id="PIRSR600823-5"/>
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        <source>
            <dbReference type="RuleBase" id="RU362060"/>
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        <source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0718990.1"/>
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    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    <sequence length="320" mass="34009" checksum="9B6168848AC80413" modified="2011-05-31" version="1">MAATVVRCLLMAAAVLAPLLAGSEGAFLSSKFYDRRCPSLQAIVRSVMTQAVAAEPRMGASILRLFFHDCFVNGCDASILLDDTANFTGEKNAGPNANSVRGYDVIDTIKAQVEAACKATVSCADIVALAARDSVNLLGGPAWAVQLGRRDARNASQSAANSNLPSPGSSLASLIATFGSKGLSPRDMTALSGAHTIGQSRCATFRDRIYNDTNIDPKFAALRKQTCPQTGGDAALAPIDVSTPTWFDTTYYENLANKQGLFHSDQELYNGGSQDAMVRVYMRNPDIFAGDFAKAMGKMGSLMPSADTPTEIRLDCKKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F2DQ71</accession>
    <name>F2DQ71_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123444316</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
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        <property type="molecule type" value="mRNA"/>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="711"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="790"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="34"/>
            <end position="328"/>
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    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
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            <begin position="363"/>
            <end position="599"/>
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            <end position="957"/>
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    <feature type="domain" description="ABC transporter" evidence="12">
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            <begin position="994"/>
            <end position="1230"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="643"/>
        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285940.2"/>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1232" mass="134412" checksum="FD9AF372AF94C95D" modified="2011-05-31" version="1">MSPGTAATGGGGGERRSIRGLFKFADRVDVVLMALGTLGAIGDGCSTNLLLIFASDVMNSLGRGHAQQQGSATSAHFMHDIEKSCLNFVYLAFAILVVASMEGYCWSRTSERQVLRIRHLYLEAILRQEVAFFDSQEATTSEIINSISKDASLIQEVLSEKVPLFLMHSTVFVSGLAFSTYFSWRLALVSYPLVLLLIIPGLIYGKYLLYLSRESRREYAKANSLVEQALGSIKTVYSFTAEKGIIQRYTAILDKTINLGIKQGIAKGLAVGFTGLSFAIWAFLAWYGSRLVMYHHESGGRIYAAGISFVLGGLSLGMALPELKHFIEASVAATRILERINRVPQINDDDPKGLVLDQVRGEIEFESIRFVYPSRPNMTVLKDFNLQIPAGQTIALVGSSGSGKSTAIALVQRFYDASEGTVKVDGIDIKKLNLKSIRSKMGLVSQDHALFGTSIKENILFGKPDATMDELYAAAMTANAHNFIMGLPEGYETKIGERGALLSGGQKQRIAIARAVLKNPAILLLDEATSALDSESEKLVQHALDQASMGRTTLVVAHKLSTVKNADQIAVVDGGRIAEIGTHDELINKGGPYSRLVKLQKMVSYIDQETDQFRASSAARTSASRLSMSRASPMPLTPGFSKETESYVSPPAPSFSRLLAMNAPEWKQALIGSISALVYGSLQPTYALTIGGMIAAFFVQDHNEMNAIISRYALIFCSLSLVSIAVNLLQHYNFAYMGEHLVRRIRVQVLEKILTFEAAWFDEDTNSSGSLCSRLSDESSLVKTLVADRISLLLQTACGIVIAVTMGLIVAWKLALVMIAVQPCTMICYYAKKIVLSNVSRDLAKAQYESTQIAIEAVYNHRMVTSFGCSSKILQLFEHTQEEPLRKARKKSWVAGITTGLSPCLTFLSWALDFWYGGKLAQSGEISAGDVFKTFFVLVSTGKLIADAGSMTSDLAKGANAVASVFEVLDRKSISPQNSQVEKDNPKSKIQGRIEFKKVDFSYPTRPQCLILQDFSLDVKAGTSIGLVGRSGCGKSTIIGLIQRFYDVDRGAVRIDGVDVREMNVLWYRGFTALVSQEPAMFSGSVRDNIAFGKPEADEEEIVEAAKAANAHEFISSLKDGYDTDCGEHGIQLSGGQKQRIAIARAIIRNPAILLLDEATSALDAQSEQVVQEALDRIMTGRTTIIVAHRLNTIKNADSIAFLGEGKVIERGTYPQLMNKKGAFFNLATLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2DVQ0</accession>
    <accession>M0XGL6</accession>
    <name>F2DVQ0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123452720</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Flower</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EMBL" id="AK367968">
        <property type="protein sequence ID" value="BAJ99171.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK376707">
        <property type="protein sequence ID" value="BAK07902.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985367.1">
        <property type="nucleotide sequence ID" value="XM_045129432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DVQ0"/>
    <dbReference type="SMR" id="F2DVQ0"/>
    <dbReference type="PaxDb" id="4513-MLOC_59863.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519500"/>
    </dbReference>
    <dbReference type="GeneID" id="123452720"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519500"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452720"/>
    <dbReference type="eggNOG" id="KOG4175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_1"/>
    <dbReference type="OrthoDB" id="665873at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DVQ0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000107">
        <property type="entry name" value="Tryptophan synthase alpha chain, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF9">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ99171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK07902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="343" mass="35594" checksum="9D7C85B67EC028A0" modified="2011-05-31" version="1">MAFALKPCLSPSSSPPSSLAAATLPMKPVFVGALAVDPAMATPAAPGRHAVRARAAATTLAPTPAPVPAGNGGLSVAKAMSRARDNGMTAFIPYITAGDPDLATTAEALRLLDRLGADVIELGLPFSNPSADGPVIQASTARALSAGATSDTVMAMLKEVTPELSCPVVILSYFNPIERRGTGSFIAAAREAGVRGLIVPDLPYTEAGVLSTEAKKNDIELVLLTTPSTKPERMNEITAASEGFLYLVSINGLTGARPDVNPRVKDLLREIKQVTDKAVAVGFGISTPDHVRQVAQWGADGVIIGSAMVKQLGEADSPRKGLKRLEVYARSLKDALHAVICTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F2DXA6</accession>
    <name>F2DXA6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-aspartyl-peptidase</fullName>
            <ecNumber evidence="3">3.4.19.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123428128</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="3"/>
    <dbReference type="EMBL" id="AK368524">
        <property type="protein sequence ID" value="BAJ99727.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968227.1">
        <property type="nucleotide sequence ID" value="XM_045112292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DXA6"/>
    <dbReference type="SMR" id="F2DXA6"/>
    <dbReference type="MEROPS" id="T02.A02"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204250"/>
    </dbReference>
    <dbReference type="GeneID" id="123428128"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0169850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0169850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0169850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0204250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0204250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0204250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428128"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04701">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF13">
        <property type="entry name" value="ISOASPARTYL PEPTIDASE_L-ASPARAGINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="231"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="253"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="9">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ99727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0204250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="333" mass="35080" checksum="959481452BCFC355" modified="2011-05-31" version="1">MARWAIAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELETDPCFNSGRGSALTRAGTVEMEASIMDGRGRRCGAVSGVSTVRNPVSLARRVMDKSPHSYLAFDGAEDFARDQGLEVVDNSYFITEENVGMLKLAKEANSILFDYRIPLAGADTCSAQAAATENHNNNGMVMNGLPISIYAPETVGCAVVDCNGFTAAATSTGGLMNKMTGRIGDSPLIGAGTYACGHCAVSCTGEGEAIIRSTLARDVAAVMEYKGLPLQEAVDFCVKERLDEGFAGLIAVSGTGEVAYGFNCTGMFRGCATEDGFMEVGIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2DYA4</accession>
    <name>F2DYA4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123428479</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AK368872">
        <property type="protein sequence ID" value="BAK00076.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044968624.1">
        <property type="nucleotide sequence ID" value="XM_045112689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DYA4"/>
    <dbReference type="SMR" id="F2DYA4"/>
    <dbReference type="PaxDb" id="4513-MLOC_4572.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0214020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214020"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0214020.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214020.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214020"/>
    </dbReference>
    <dbReference type="GeneID" id="123428479"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0214020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0214020.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0214020.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0214020"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428479"/>
    <dbReference type="HOGENOM" id="CLU_000288_15_0_1"/>
    <dbReference type="OMA" id="NTGYKDD"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DYA4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF47">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="61"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAK00076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0214020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="494" mass="55400" checksum="DDF0E7E0805DB0ED" modified="2011-05-31" version="1">MGARVSKATSCCCLRGQLHGSTRLDDPGSEEDEQGEAYELPAFQEYTFEQLRLATAGFAVDNIVSEHGEKAPNVVYKGKLDAQRRIAVKRFNRSAWPDPRQFLEEAKSVGQLRSKRLANLLGCCCEGDERLLVAEYMPNDTLAKHLFHWETQAMKWPMRLRVVLYLAEALEYCTSKGRALYHDLNAYRVLFDDDCDPRLSCFGLMKNSRDGKSYSTNLAFTPPEYMRTGRITPESVIYSFGTLLLDVLSGKHIPPSHALDLIRDRNFSMLTDSCLEGQFSNEEGTELVRLASRCLHYEPRERPNVRSMVQALAPLQKDVETPSFELMDMPQAGASSVQSLPLSPLADACSRKDLTAIHEILEKTGYKDDEGTANELSFQMWTNQMQDTLTSKKKGDSAFRQKDFTTAIDCYSQFIEVGTMVSPTIYARRCLSYLMNDMAEQALSDAMQALVISPTWPTAFYLQAAALLSLDMENEAQDALKDGCAQETSSSSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F2E110</accession>
    <name>F2E110_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123407507</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK369831">
        <property type="protein sequence ID" value="BAK01032.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956587.1">
        <property type="nucleotide sequence ID" value="XM_045100652.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956588.1">
        <property type="nucleotide sequence ID" value="XM_045100653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E110"/>
    <dbReference type="STRING" id="112509.F2E110"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643690"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643690.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643690.2"/>
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    </dbReference>
    <dbReference type="GeneID" id="123407507"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0534770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0534770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0534770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0643690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643690.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0643690.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643690.2"/>
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    </dbReference>
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    <dbReference type="eggNOG" id="KOG2325">
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    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14479">
        <property type="entry name" value="SPX-MFS_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051068">
        <property type="entry name" value="MFS_Domain-Containing_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045264">
        <property type="entry name" value="SPXM_SPX_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YAJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510:SF78">
        <property type="entry name" value="SPX DOMAIN-CONTAINING MEMBRANE PROTEIN OS06G0129400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="567"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="470"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK01032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="700" mass="77477" checksum="B34D244D72E13EBE" modified="2011-05-31" version="1">MVRFGKKLMADQVPEWRGYYINYKLMKKKVKQYGQQVQQGEKDRRRVLKDFSKMLDDQIETIVLFLLEQQGRLASRIEKLGKQRAILAEQPDISAIAELREAYREVGLDLIKLLKFVDLNATGIRKILKKFDKRFSYRFTDYYVSSRSNHPYSQLQQVFKHVGVGAVVGALSRNLADLQERQGSYLSIYDQPSSALKDPIIDMINSSVDRLTRSTNFLRFLGQHALIAQEESPSTAGEEEIEDQKYHFVSLMLNLVNTFLYMVNTYIIVPTADDYSVSLGAASTVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIVLFLGNVCYAMAYDTKSLTVLIVGRLLCGFGSARAVNRRYISDCVPARIRMQASAGFVSASALGMACGPALAGLLQWRFKIYMVTFSQSTLPGWLMAVAWLLYLVWLCISFKEPNRSTEADDTPHNRASGQSVDIGQVENGLAQPLLGDSESKQIEDDDDDEEDGSEESVQGSRGPATSIGSAYRLLTPSVKVQLLIYFMLKYAMEILLSESSVITNHYFNWNTSSVAIFLAILGLTVLPINAVVGTYISNMFEDRQLLMASQITLLVGIIFSFKVTSTYSVIQYVVSALITFVSAEVLEGVNLSLLSSVMSSRLSRGTYNGGLLSTEAGTLARVVADCTITAAGYLGIGSLLNVTLLPSLVICAASIACTFLTYNSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F2E2M2</accession>
    <name>F2E2M2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123425395</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK370393">
        <property type="protein sequence ID" value="BAK01594.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965025.1">
        <property type="nucleotide sequence ID" value="XM_045109090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E2M2"/>
    <dbReference type="STRING" id="112509.F2E2M2"/>
    <dbReference type="PaxDb" id="4513-MLOC_11070.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127050"/>
    </dbReference>
    <dbReference type="GeneID" id="123425395"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0104840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0104840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0104840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0127050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0127050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0127050"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425395"/>
    <dbReference type="eggNOG" id="ENOG502S4KK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129558_0_0_1"/>
    <dbReference type="OMA" id="KLENMGR"/>
    <dbReference type="OrthoDB" id="62622at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010005">
        <property type="term" value="C:cortical microtubule, transverse to long axis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403:SF48">
        <property type="entry name" value="PROTEIN SPIRAL1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403">
        <property type="entry name" value="SPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK01594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0127050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="100" mass="9813" checksum="E8CA80D970661B73" modified="2011-05-31" version="1">MSRGGSAGGGQSSLGYLFGDGEPAKPAAAPAAKAPPAEKPAQAPADVAKQVPAGIPGSRANNYYRADGQNTGNFLTDRPSTKVHAAPGGGSSLGYLFGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2E4J5</accession>
    <name>F2E4J5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123425802</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 18 family. Xylanase inhibitor subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="BAK02267.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044965475.1">
        <property type="nucleotide sequence ID" value="XM_045109540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E4J5"/>
    <dbReference type="SMR" id="F2E4J5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0138340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0138340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0138340"/>
    </dbReference>
    <dbReference type="GeneID" id="123425802"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0113880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0113880.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0113880"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0138340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0138340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425802"/>
    <dbReference type="HOGENOM" id="CLU_007818_0_0_1"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitinase III C10701-rice"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF9">
        <property type="entry name" value="XYLANASE INHIBITOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044730848" evidence="7">
        <location>
            <begin position="31"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="8">
        <location>
            <begin position="36"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK02267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0138340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="304" mass="33400" checksum="BB8834D0FD946518" modified="2011-05-31" version="1">MAPLPPWRPACLLPLLSVVAALFLTPPALAAGGKTGQVTVFWGRNKAEGSLREACDSGMYTMVTMSFLDVFGANGKYHLDLSGHDLSSVGADIKHCQFKGVPVSLSIGGYGTGYSLPSNRSALDLFDHLWNSYFGGSKPGVPRPFGDAWLDGVDLFLEHGTPADRYDVLALELAKHNIRGGPGKPLHLTATVRCGYPPAAHVGRALATGIFERVHVRTYESDNGCNQNFGWEGSWNEWTAAYPATRFYVGLTADDKSYQWVHPKNVYYSVAPVSQKKDNYGGIMLWDRYFDKQTNYSSLIKYYA</sequence>
</entry>
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    <accession>F2E539</accession>
    <name>F2E539_HORVV</name>
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        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123410430</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AK371263">
        <property type="protein sequence ID" value="BAK02461.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959344.1">
        <property type="nucleotide sequence ID" value="XM_045103409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E539"/>
    <dbReference type="STRING" id="112509.F2E539"/>
    <dbReference type="PaxDb" id="4513-MLOC_59232.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0014680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0014680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0014680"/>
    </dbReference>
    <dbReference type="GeneID" id="123410430"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0011260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0011260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0011260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0014680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0014680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0014680"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502QT0N">
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    </dbReference>
    <dbReference type="OMA" id="WWRWLHD"/>
    <dbReference type="OrthoDB" id="620353at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E539">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK02461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0014680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="290" mass="32761" checksum="2E0EB9C6ABE71212" modified="2011-05-31" version="1">MDGMYYPQRFSNMMIGYLNLATLLASIPVIGAGLWLAKGSTTTCSSMLQTPLLIVGFVVLLISLAGFVGACFHVAWALWLYLFAIMLLIGMLLGLTMFGFAVTAGGGGTQVPGRPYREYHISDYSSWLQKHMQDVKYWKPALACVVGSKACPKIANWTPMDYLQHDLTPIQSGCCKPPTSCTYSGGMPVGAQDEDCYQWNNAPNILCYQCNSCKAGVMEQVRQDWHKISVLNVIVLVFLICVCACGCCAFRNARRSISEYPYGVNRMSKINPRWDYYWWRWFRDRREQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F2E695</accession>
    <name>F2E695_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123409705</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in sporophytic self-incompatibility system (the inability of flowering plants to achieve self-fertilization).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AK371669">
        <property type="protein sequence ID" value="BAK02867.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044958502.1">
        <property type="nucleotide sequence ID" value="XM_045102567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E695"/>
    <dbReference type="SMR" id="F2E695"/>
    <dbReference type="STRING" id="112509.F2E695"/>
    <dbReference type="PaxDb" id="4513-MLOC_61675.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726350"/>
    </dbReference>
    <dbReference type="GeneID" id="123409705"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0602410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0602410.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0602410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0726350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0726350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0726350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409705"/>
    <dbReference type="eggNOG" id="ENOG502QU25">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042865_0_0_1"/>
    <dbReference type="OMA" id="PYKDLGS"/>
    <dbReference type="OrthoDB" id="590879at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035446">
        <property type="entry name" value="SLSG/EP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF100">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002686">
        <property type="entry name" value="SLG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090860" description="non-specific serine/threonine protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="12">
        <location>
            <begin position="37"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="467"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="488"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK02867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0726350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="503" mass="52919" checksum="D7DFC6BCF28070A6" modified="2011-05-31" version="1">MAGELTVMICMAAAALLISLTGGAGVAAASAKELRRGFSATHDTSYSHFQPVLTDPTGAFAFGFLRVNSTTLDLAVLHLPSAFPLWRAMPDRPAPWSAAASLSFNGNLVLTDAATNQVLWSTAAAAGDRAVLLNTSNLQIRSSSSPVAVWQSFDHPSDTIVQGQNLTSSAALHSIDQRFAMRLGSNYFALYVEPPPLSSGSVAAAMYSRHTALEAKAQIVAGGGPIYVRVEADGYLGMYQKEGAPADVMSFDTFNHGVRALRRMTLEPDGNLRAYYWDGSRWVLDYTTITDSCELPTTCGAYSVCVQPSGRCACLANATDGPGCAAPSVGSGLCGTTGGEVGGLYSEVRRHGVEPANKELLGFEHAPSAGDCEALCARNCSCWGAVYSNGTGYCYLMDYPAQLMVAADKRKVGYFKVRSMEEAAARGGRAGGVKAALLAVGVAVVAAAAAFGAYRVWDRRRRAAAETRQQLGADGDGLSPGPYKNLGSFSSVELSSSFNSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2EA83</accession>
    <name>F2EA83_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123399662</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Seed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ELIP/psbS family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373058">
        <property type="protein sequence ID" value="BAK04255.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949990.1">
        <property type="nucleotide sequence ID" value="XM_045094055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EA83"/>
    <dbReference type="STRING" id="112509.F2EA83"/>
    <dbReference type="PaxDb" id="4513-MLOC_26864.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481890"/>
    </dbReference>
    <dbReference type="GeneID" id="123399662"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0399450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0399450.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0399450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0481890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0481890.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0481890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399662"/>
    <dbReference type="OMA" id="FAYTVAM"/>
    <dbReference type="OrthoDB" id="765963at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EA83">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK04255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0481890.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="17133" checksum="412D3E2446A090E7" modified="2011-05-31" version="1">MATMMAMSSFAGAAVLPRGSARSLPALGRRTLVVRAQTEGPNAPPPNKPKASTSIWDAMAFSGPAPERINGRLAMVGFVTALAVEAGRGDGLLSQLGSGTGQAWFAYTVAMLSMASLVPLLQGESAEGRAGAIMNANAELWNGRFAMIGLVALAATEIITGTPFINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>F2EIY5</accession>
    <name>F2EIY5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123452638</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="AK376112">
        <property type="protein sequence ID" value="BAK07307.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044985270.1">
        <property type="nucleotide sequence ID" value="XM_045129335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EIY5"/>
    <dbReference type="SMR" id="F2EIY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0459670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0459670"/>
    </dbReference>
    <dbReference type="GeneID" id="123452638"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0380490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0380490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0380490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0459670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0459670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0459670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452638"/>
    <dbReference type="OrthoDB" id="1601230at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013097">
        <property type="entry name" value="Dabb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044662">
        <property type="entry name" value="HS1/DABB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33178">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33178:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07876">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_R_A_B_BARREL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stress-response A/B barrel" evidence="2">
        <location>
            <begin position="3"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK07307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0459670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="106" mass="11681" checksum="9424E3E5D1C5D350" modified="2011-05-31" version="1">MEFKHLCLVRFKEGVVVDDIIQQLTKLAAELDTVKFFGWGKDVLNQETLTQGFTHVFSMSFASAEDLAACMGHEKHSAFAATFMAVLDKVVVLDFPFVVAKPAPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2ELJ2</accession>
    <name>F2ELJ2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123411593</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Flower</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK377020">
        <property type="protein sequence ID" value="BAK08214.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960494.1">
        <property type="nucleotide sequence ID" value="XM_045104559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ELJ2"/>
    <dbReference type="SMR" id="F2ELJ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0711350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0711350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0711350"/>
    </dbReference>
    <dbReference type="GeneID" id="123411593"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0590000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0590000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0590000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0711350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0711350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0711350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411593"/>
    <dbReference type="OrthoDB" id="72851at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000089">
        <property type="entry name" value="Cellulose synthase-like protein D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="772"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="808"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="904"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="932"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="962"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="594"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAK08214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0711350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="996" mass="111266" checksum="4E52C94FA6D17EC0" modified="2011-05-31" version="1">MSSDYTNYTVFMPPTPDNQPGAAPAPASGGSTKPDNLPLPRYSSGSKLVNRRSGDDGAATAGGAKMDRRLSTSHVASPSKSLLVRSQTGEFDHNRWLFETQGTYGIGNAYWPQDDNDDGAGMGGGSVKMEDLVDKPWKPLSRKVPIPPGILSPYRLLVLVRFVALSLFLIWRATNPNPDAMWLWGISIVCEYWFAFSWLLDQMPKLNPINRAADLAALREKFESKTPSNPTGRSDLPGLDVFISTADPYKEPPLVTANTLLSILATDYPVEKLFVYISDDGGALLTFEAMAEACAYAKVWVPFCRKHSIEPRNPEAYFTQKGDPTKGKKRPDFVKDRRWIKREYDEYKVRINDLPEAIRRRAKAMNAQERKIARDKAAASSDAAPAPVKATWMADGTHWPGTWLDSAPDHGKGDHASIVQVMIKNPHHDVVYGDADDHAYLDFTNVDVRIPMFVYLSREKRPGYDHNKKAGAMNAMVRASAILSNGPFMLNFDCDHYVYNCQAIREAMCYMLDRGGDRICYIQFPQRFEGIDPSDRYANHNTVFFDGNMRALDGLQGPMYVGTGCLFRRYAIYGFNPPRAVEYHGVVGQTRVPIDPHARSGDGVPDELRPLSDHPDHEAPQRFGKSKMFIESIAVAEYQGRPLADHPSVRNGRPPGALLMPRPPLDAATVAESVSVISCWYEDNTEWGLRVGWIYGSVTEDVVTGYRMHNRGWRSVYCITKRDAFRGTAPINLTDRLHQVLRWATGSVEIFFSKNNALLASRRLMFLQRMSYLNVGIYPFTSLFLIMYCLLPALSLFSGQFIVATLDPTFLCYLLLISITLMLLCLLEVKWSGIGLEEWWRNEQFWVIGGTSAHLAAVLQGLLKVAAGIEISFTLTAKAAAEDDDDPFAELYLIKWTSLFIPPLAIIGINIIAMVVGVSRCVYAEIPQYSKLLGGGFFSFWVLAHYYPFAKGLMGRRGRTPTIVYVWAGLISITVSLLWITISPPDDRVSQSGIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>M0VBL1</accession>
    <name>M0VBL1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123430832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970605.1">
        <property type="nucleotide sequence ID" value="XM_045114670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VBL1"/>
    <dbReference type="PaxDb" id="4513-MLOC_21840.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196660"/>
    </dbReference>
    <dbReference type="GeneID" id="123430832"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0163220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0163220.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0163220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0196660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430832"/>
    <dbReference type="eggNOG" id="ENOG502QRUH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="LELQFWS"/>
    <dbReference type="OrthoDB" id="1907510at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF59">
        <property type="entry name" value="PURINE PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="391" mass="41779" checksum="0FD811E2B2C0D71A" modified="2013-04-03" version="1">MGAAGEIHLQIAGTRGEQGAEGVADNGTSPATAAPAPPSMSERVQWWAVVLVNIVLVLAGQSVANLLGRIYYDQGGGSLWIATVVQSCGTPLAIPLLLYFRRRPKAATTAVTRPPLIKISAIYAGLGVLLAGDNLMYSYALLYLPLSTYSLICATQLSFNAVFSYFLNKQKFTALILNSVVLLTFSAALVGVSHGSDGTNSSVPAGKFPLGFALTLSASALFSLILSLNQLTFDKVLRSDTLYDVMEMQFWSNTAAAVVSVAGLFISGEWSALHGEMDGYRKGRLAYGMTLAWTAISWQLTTMGLMGLVAAVSSLFTNVISTVGLPLSPIIAVIFLGDRMDGVKVLAMLVAVWGFLSYIYQHYLDDAKVKKNLAERSADDDEHQTVKLATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>M0WNJ8</accession>
    <name>M0WNJ8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123445374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978282.1">
        <property type="nucleotide sequence ID" value="XM_045122347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WNJ8"/>
    <dbReference type="FunCoup" id="M0WNJ8">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="PaxDb" id="4513-MLOC_53222.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314570.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314570"/>
    </dbReference>
    <dbReference type="GeneID" id="123445374"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262820.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0314570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314570.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314570"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445374"/>
    <dbReference type="eggNOG" id="ENOG502R7VM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WNJ8"/>
    <dbReference type="OrthoDB" id="622335at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WNJ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="2">
        <location>
            <begin position="127"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314570.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="417" mass="45699" checksum="13B1953522BFCD56" modified="2013-04-03" version="1">MSSHRRNPSPSLQKIPQNMDCDRRAPAASSVLGDDDLLREILIRLGFPNLLVRAALVSKRWLLHASDPVFLRRFRDRNPPRVLGVCAGYSARPYKFVPLPQPPELAALSRRAASSCADAFALGGQRIEHCRNGRLIVDSLRRGTFNHSVLAPLLSGEPEAVLPPIPPPGRHPGQPAQGAFTHIFLPEDGGCNGITLVNLWKVQREVRAEVYVLGAGGWGAPSTAVTEIELQYAATFFAKMLPPVHGKVFMVTGFGYTLGLDLAAARFVMLELPAGVQYNYMLSCAEGSGIYLVTADRFQLSVWLHQMTGDNHGAGGWLLVDTFSVLEACIRLAGDNWVSYEYVNVVVVGDNADFVFLDHPASGVLFYVHRSSRVVKKVYQRTADEHVCRGVAVRISPVMTTWPPIFPALSIGHDQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="1998-01-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>O24613</accession>
    <accession>Q6RYE9</accession>
    <name>O24613_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucellin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123447528</name>
        <name evidence="6" type="ORF">615K1.6</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="1997" name="Plant Mol. Biol." volume="35" first="821" last="831">
            <title>Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration.</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Foolad M.R."/>
            </authorList>
            <dbReference type="PubMed" id="9426602"/>
            <dbReference type="DOI" id="10.1023/A:1005833207707"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2002" name="Mol. Genet. Genomics" volume="268" first="488" last="499">
            <title>Cereal genes similar to Snf2 define a new subfamily that includes human and mouse genes.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Echenique V."/>
                <person name="Busso C."/>
                <person name="SanMiguel P."/>
                <person name="Ramakrishna W."/>
                <person name="Bennetzen J.L."/>
                <person name="Harrington S."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="12471446"/>
            <dbReference type="DOI" id="10.1007/s00438-002-0765-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2004" name="Science" volume="303" first="1640" last="1644">
            <title>The wheat VRN2 gene is a flowering repressor down-regulated by vernalization.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Loukoianov A."/>
                <person name="Blechl A."/>
                <person name="Tranquilli G."/>
                <person name="Ramakrishna W."/>
                <person name="SanMiguel P."/>
                <person name="Bennetzen J.L."/>
                <person name="Echenique V."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="15016992"/>
            <dbReference type="DOI" id="10.1126/science.1094305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="5">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="6">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="U87148">
        <property type="protein sequence ID" value="AAB96882.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U87149">
        <property type="protein sequence ID" value="AAB96883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY485643">
        <property type="protein sequence ID" value="AAS58479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T06213">
        <property type="entry name" value="T06213"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980072.1">
        <property type="nucleotide sequence ID" value="XM_045124137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O24613"/>
    <dbReference type="SMR" id="O24613"/>
    <dbReference type="MEROPS" id="A01.073"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416260"/>
    </dbReference>
    <dbReference type="GeneID" id="123447528"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0347000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0347000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0347000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416260"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="O24613">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05475">
        <property type="entry name" value="nucellin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033823">
        <property type="entry name" value="Nucellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF609">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015096749" evidence="3">
        <location>
            <begin position="24"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="38"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAB96883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAS58479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="410" mass="45032" checksum="FAB06149C8F4C1D6" modified="1998-01-01" version="1">MAAMWSPIIGLLLLLLPLGPSSAIKFPLEGNVYPVGHFYATLNIGEPAKPYFLDVDTGSNLTWLECHHPVHGCKGCHPRPPHPYYTPADGNLKVVCGSPLCVAVRRDVPGIPECSRNDPHRCHYEIQYVTGKSEGDLATDIISVNGRDKKRIAFGCGYKQEEPADSPPSPVDGILGLGMGKAGFAAQLKGHKMIKENVIGHCLSSKGKGVLYVGDFNPPTRGVTWAPMRESLFYYSPGLAEVFIDKQPIRGNPTFEAVFDSGSTYTHVPAQIYNEIVSKVRGTLSESSLEEVKGRALPLCWKGKKPFGSVNDVKNQFKALSLKITHARGTNNLDIPPQNYLFVKEDGETCLAILDASLDPVLKELNFILIGAVTMQDLFVIYDNEKKQLGWVRAQCDRVQELESVIDSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GAZ3</accession>
    <name>A0A287GAZ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123447061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044979563.1">
        <property type="nucleotide sequence ID" value="XM_045123628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287GAZ3"/>
    <dbReference type="SMR" id="A0A287GAZ3"/>
    <dbReference type="STRING" id="112509.A0A287GAZ3"/>
    <dbReference type="PaxDb" id="4513-MLOC_72532.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0077190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0077190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0077190"/>
    </dbReference>
    <dbReference type="GeneID" id="123447061"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0062600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0062600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0062600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0077190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0077190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0077190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447061"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287GAZ3"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287GAZ3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000100">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000001">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000046">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000039">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011071">
        <property type="entry name" value="PRK14501.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="843"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="863"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0077190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="980" mass="108939" checksum="CD65C8D8F0212B3A" modified="2017-11-22" version="1">MSSAVQRRSSVRSGDPRADAAMPVPSSNAPNSSPFSNDGGGSPNGIDRVLRGSARRNHLPTDASPDAMETDVAEPAAAFLGPHANMDDAGGGGSGGHAARPPLSGPRNGFRRLGLRGMKQRLLVVANRLPVSANRRGEDQWSLEISAGGLVSALLGVKDVDAKWIGWAGVNVPDEVGQRALTSALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRNFESQFDAYKRANQMFADVVYEHYQEGDVIWCHDYHLMFLPKCLKEHDINMKVGWFLHTPFPSSEIYRTLPSRSELLRSVLCADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRVAAFPIGIDSERFKRALDIAAVRRHVTELTQRFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPEWNDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEYVACQGSKKGVLILSEFAGAAQSLGAGAILVNPWNITEVADSIKHALTMTSDEREKRHRHNYAHVTTHTAQDWAETFVCELNDTVAEALMRTRQVPPDLPSRTAIQKYLQSKNRLLILGFNSTLTEPVESSGRRGGDQIKEMELKLRPDLKGPLRALCEDESTTVIVLSGSDRSVLDENFGEFNLWLAAEHGMFLRPTDGEWMTTMPEHLNMDWVDSVKHVFEYFTERTPRSHFEHRETSFVWNYKYADVEFGRLQARDMLQHLWTGPISNAAVDVVQGSRSVEVRSVGVTKGAAIDRILGEIVHSKSMVTPIDYVLCIGHFLGKDEDIYVFFDPEYPSSESKVKPDGASVSVDRRQNGRPSNGRSGSRNSQARTPKPQAAPLPQPPPERSSSSSDHGMGNNNNQHDWREGSSVLDLNGDNYFSCAVGRKRSNARYLLNSSEDVVSFLKEMAESTTPRAAGLQPGAAADYMFLDRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MWZ8</accession>
    <name>A0A287MWZ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A287MWZ8"/>
    <dbReference type="SMR" id="A0A287MWZ8"/>
    <dbReference type="FunCoup" id="A0A287MWZ8">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287MWZ8"/>
    <dbReference type="PaxDb" id="4513-MLOC_4629.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332630"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287MWZ8"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287MWZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010182">
        <property type="term" value="P:sugar mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000193">
        <property type="entry name" value="Serine/threonine-protein kinase CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF292">
        <property type="entry name" value="OS09G0566550 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="502"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="773" mass="84023" checksum="60EEA10FD2D67E2B" modified="2017-11-22" version="1">MELPAAGGGRRTSYSLLSQFPDDAGGGTTGASPAVLQRQSSGGSSYGAGSSVSASSDYPFHLPPATAAVGGGGGGGGTPSPCKSWAQQAEETYQLQLALALRLCADAACAADPGFLDPGDSAGSKMGGGGSGSGRAFPLAPPSPTAEALSHRFWVNGSLSYSNTIPDGFYLIQGMDPFVWSMCTDVHEENRIPSVESLKSVRPDDSSIQVVLVDRRADFDLGMLENYASSFLSSSSDMKDVINQLAKLVSSRMGGTTSNEENLLPRWKESSEAIKSSAGSIVLHLGKLPIGLCKHRSLLFKMLADKVNIPCRLVKGCKYCKAEDASSCVVRFGLEREYLVDLFGAPGQLSDPDSFVNGPYSLSVPSPLRPPKFRSLEITSNFSSVAKQYFSDCHSLNLLFSDASTGASSGAAVPVDQMYSKKHDAGDGIANSWVPVKGQAIANPDIILPEAPREILPLMTPSNLTADKKKEFQLIEGNQYLRSTVSDLSLAVDDLIIPWSELVLKEKIGAGSFGTVHRADWHGSDVAVKILMEQDYHLDRFKEFMREVAIMKSLRHPNIVLFMGAVTEPPNLSIVTEYLSRGSLYKLLHRSGAREVLDERRRLNMAFDVAKGMNYLHRRSPPIVHRDLKSPNLLVDKKYTVKVCDFGLSRLKANTYLSSKSLAGTPEWMAPEVLRDEPSNEKSDVYSFAVILWELMTLQQPWCNLNPAQVVAAVGFKGRRLEIPKDLNPQVAALIESCWINEPWRRPSFANIMETLRPLINKVPVPQLIRSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287TNI5</accession>
    <name>A0A287TNI5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor GAMYB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123405130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="7">subsp. vulgare</strain>
        </source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0040880.1.CDS1"/>
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        <property type="component" value="Chromosome 1H"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CLCR"/>
    </dbReference>
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        <property type="entry name" value="Vwaint_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF132">
        <property type="entry name" value="OS10G0464800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWA"/>
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        <property type="entry name" value="Vwaint"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
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    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="175"/>
            <end position="360"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0040880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="639" mass="68318" checksum="6B9389ED9C496190" modified="2022-05-25" version="1">MESNSSTCGICNGDMRRGGGASASGFTADCSHQFHYRCVSGSLASSRQACPLCSARCRDLPSFRSSKSKSSTPSQPPHASRAPAQPFFRPMEPRVFDDDDPVVRAPRPLGDRHHGAASATSSGSLAVALSTHCEYSSLARDAPHENFAVLVHARAPGTGGTATGGTPPRARAPIDLVTVLDVSGSMVGSKLALLKQAMGFVVDSLGPADRLCVVSFSSGASRLMRLARMSESDGGKDLAKRAVQSLAASGGTNIGAALRKAAKVLDDRLHRNAVASVILLSDGQDTYTVPKRGRDANYDALVPPSFMYTGAGDRSAPVHTFGFGTDHDAAAMHTIAEATGGTFSFIENETDIQDAFAQCIGGLLSVAVQELRVDISCPQSGVRVRSVNSGCYKSHVDVDGRSASIDVGELYADEERRFLLLMDVPRARSTTDVTRLMQVSCAYRDMVTGRPTNVDGEDAVVQRPSRAVSQERSAEVERERVRLAATDGIAAARAAAERGAHEEAAEILQSRQRAVARSAAARAGDSMCVELSRDLQEMRARVADRRRYELSGRAYMLAGLSSHAQQRATSRQMSGDSETTRRRAEAALGTTVISTTGATASYSYVTPAMMDMLDRSRRSREMTALSQQRSKERRRSAHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WCB3</accession>
    <name>A0A8I6WCB3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123431313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds double-stranded RNA.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971073.1">
        <property type="nucleotide sequence ID" value="XM_045115138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WCB3"/>
    <dbReference type="SMR" id="A0A8I6WCB3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0043800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0043800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0043800"/>
    </dbReference>
    <dbReference type="GeneID" id="123431313"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0033960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0033960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0033960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0043800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0043800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0043800"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431313"/>
    <dbReference type="OrthoDB" id="5988181at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd19907">
        <property type="entry name" value="DSRM_AtDRB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19908">
        <property type="entry name" value="DSRM_AtDRB-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000036">
        <property type="entry name" value="Double-stranded RNA-binding protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044450">
        <property type="entry name" value="AtDRB-like_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044451">
        <property type="entry name" value="AtDRB-like_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031:SF26">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="6">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="139"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="196"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="474"/>
            <end position="507"/>
        </location>
    </feature>
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        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="474"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0043800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="527" mass="56848" checksum="5C8FA963A1001289" modified="2022-05-25" version="1">MYKNQLQELAQRSCFNLPAYTCLREGPDHAPRFKAAVIFNGEQFESPGFFTTLRQAEHAAAEVALAALARRGPSYSLAARILDETGVYKNLLQEVAQRVGAPLPSYTTERSGLGHLPVFTCTVELAGITFTGDHAKNKKQAEKNAASAAWSSLKQLAREEANSTNEPENNDEQEQIRIARALLNYRLKEKMAMANNPHASPFPKKFPMQPERKPCFGQSSQSSYSKILPLFRPKSSSRSRPESPAAADGASPSPFWPIESSNSRSRFPAAEAAPYVPVGHYHMSCHSMAPPVTVRSSIPVFSAPPLPPPSGRTQQLPPLLSNPPSVRMASPVRIRPASPHFAPSGPAQRPRPVMSVQMKDVQHKPIRESMPSAIPVQVKNAQQRTTKESPSSAMPVQVNDRHHQMFKGSPSSMMPIQMKNVQPQPPKEPLSEGKDAAPAVPLPAKMEAPAQVKEASEVVTSEVVPCPAAGSIAAATSTSISTSDCSTADPASQSGADDDAAKGEEAQKDVLEAEAALEGIIKLLEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WFQ5</accession>
    <name>A0A8I6WFQ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123427117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967007.1">
        <property type="nucleotide sequence ID" value="XM_045111072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFQ5"/>
    <dbReference type="SMR" id="A0A8I6WFQ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0178250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0178250"/>
    </dbReference>
    <dbReference type="GeneID" id="123427117"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0147460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0147460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0147460"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
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            <begin position="1"/>
            <end position="10"/>
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        <location>
            <begin position="20"/>
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        <location>
            <begin position="38"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0178250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="26258" checksum="F659EE2CA9461ECF" modified="2022-05-25" version="1">MGANGHPPPASAAAQNGSHSSGGGGGGGGGGGGGGGANPSPGGTAAALRHDPGLSREWTPEEQAILDELLVKYASDAPVIRYAKIAMKLPEKTVRDVALRCRWMNKKESGKRKKEDHSSSKKSKDKKEKVADSSSKPPVHIAGRPSVPPYSLPALPIDDDEISSKAIGGPTGELLETNAQVLSQISTNLGTMQIQDNISLLCQTRDNILRVLKEMNDAPEIMKQMPPLPVKINEELVNSILPRPTVPMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WGK8</accession>
    <name>A0A8I6WGK8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="SMR" id="A0A8I6WGK8"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0004240.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
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    </dbReference>
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            <end position="359"/>
        </location>
    </feature>
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            <begin position="1"/>
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            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0004240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="372" mass="42740" checksum="180CF167A081447C" modified="2022-05-25" version="1">MAHLEEEEEEEEEEEEEFMDMEPLFFSEAYVAKMVAEAREEKERCRREEEEKMRKEEEYRRRREAHEAVMHSIVQHDPKVDHNVYTRFFLRDFSVFDIDEESSVPPMRYTDSIYEDEFGLEDSANILSVSIVSSDVGFPVNVYGRVIARDSIDYKCIYLFHRNRDECQHLNEDGMLILTGPSRGLVLVDFIYLEIDLKIREDGVSPDRSFSKGLISIDGRVLSREKDVVVTSETLESWLSTIEVRFTTVLSAVECTFEIKLIEGLFKGNITVGIADKARSLDIEQATVIHDSTADGVVTSDESGVIKLQRSVITICLERNVMFHINNEAAGVCAERTFAFTPRRTGADEEKITCGAGKFGFRVVWSLMDFRL</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">cv. Morex</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066750.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DUF4219"/>
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        <property type="entry name" value="Znf_CCHC"/>
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        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
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        <property type="entry name" value="OS04G0629600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317:SF38">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="231"/>
        </location>
    </feature>
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        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="254" mass="27827" checksum="939DABBE1B3AE6C3" modified="2022-05-25" version="1">MALVPYAGGSGSMMSTPVPMLTGDNYTTWAIKVEADLDAARLWEAVEPPEDGAAAVIAKKDKPTRAYLFRALSEDLLLQVASKKTAAEDDTLDSFAGKLGGMAARFARLGSTLEDDALVKKLLDSVPDPLYAAVAGIEQFCDVSTMAFEEALGRLKSFDERLRRRGQAGGERVDGQLMLTAEQWRAWKRRRGGARDDDDDGKSVASSSRGNRRGRCYNCNERGHFKRDCPQRRKEPVAEQVLLADIDVENVGLL</sequence>
</entry>
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    <accession>A0A8I6WKF5</accession>
    <name>A0A8I6WKF5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC123412912</name>
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    <organism evidence="4 5">
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961809.1">
        <property type="nucleotide sequence ID" value="XM_045105874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WKF5"/>
    <dbReference type="SMR" id="A0A8I6WKF5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066110"/>
    </dbReference>
    <dbReference type="GeneID" id="123412912"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0052800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0052800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0052800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0066110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0066110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0066110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412912"/>
    <dbReference type="OrthoDB" id="2016447at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF26">
        <property type="entry name" value="OS05G0417000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="35"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0066110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="261" mass="26841" checksum="D530D703B8B7468E" modified="2022-05-25" version="1">MASSSASSFPGSVITMASSAAAAGAASSGAAGTGSPCAACKFLRRKCQPDCVFAPYFPPDNPQKFVHVHRVFGASNVTKLLNELNPYQREDAVNSLAYEADMRLRDPVYGCVGVISVLQHQLRQLQQDLARARYELSKYQAAAAVAVSASVGCNGTPAMADFIGNTVPNCTQNFINISHSTAIGAGLGFGHDQFAAVQMLARSYEGEGAVARLGVNGGSGGGYDFGYTSGMGPVSGLGPLGGGPFLKHGTAGGDERHTAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WPW7</accession>
    <name>A0A8I6WPW7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123444377</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044977040.1">
        <property type="nucleotide sequence ID" value="XM_045121105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WPW7"/>
    <dbReference type="SMR" id="A0A8I6WPW7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005790.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005790"/>
    </dbReference>
    <dbReference type="GeneID" id="123444377"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0004390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0004390.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0004390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005790.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444377"/>
    <dbReference type="OMA" id="ENGGWVA"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16987">
        <property type="entry name" value="ANTH_N_AP180_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000054">
        <property type="entry name" value="Putative clathrin assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000005">
        <property type="entry name" value="putative clathrin assembly protein At2g25430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF23">
        <property type="entry name" value="OS05G0112101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="322"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005790.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="618" mass="66034" checksum="6598EE5827673DDF" modified="2022-05-25" version="1">MSIRKALGAVKDQATIGIAKVSSAAAADLDVAVVRATAHDDGPPDERHVQEVLRLTSGSSRAHVAACVATVSRRLARTRDYVVAAKCLALLHRLVADGDPHFRHELARPAARRAGSVLAALSGFRDEAHSASWDHSAFVRAYALYLDDRVRFLLALLPAPRTVRFADHDGYGYGYGRAAGGGEAPPPDMTVSVHDMDVEGLLARGLQLRQLIDRFLACRPTGAARRSRVVLATLWPVVKESARLYEDVAVVLAVLLDRFFDMEYPDCVRAFEAHVSTARLVDDLLAFYSWCDDAGVARSSDLPNVKPIDHKLLETLEQFVRERGRAGQSSSPPPRQLQHAVQEASRSEDEHRHAEYHHMNGIKALPAPEHINAATVARPRPAQASVPPVARAPEDQTSDLVDLRESAAAADEQGNKLALALFSEAPSQSANGSWVAFPSDDGAPKETTTSAWQTPAAEPGKADWELALVETASNLSKQTAALGGGMDPLLLHGMYDQGAVRHHARAQAASGSASSVALPGTHNILALPGPDGVAGGDPFAASLAVPPPSYVQMAEMERKQELLAQEQRMWAQYRQGGMQGQAGLNGVAVGGGGSAFASNTSVPMPMPMAYHGAGAYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WTQ3</accession>
    <name>A0A8I6WTQ3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTQ3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0144420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0144420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0144420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0119000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0119000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0119000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0144420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0144420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0144420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="7"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0144420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="201" mass="23017" checksum="93BD324175302A5E" modified="2022-05-25" version="1">MYVQFIGLGGVFKTPSIKIRRVLCLAIANSYDAEQDAFIINRRACRIALEDVAHITGMPCDGKKHVPSNLDDNMELWKKLKDRNDTKITFKGLLAKMKGDSTPNFGRPFVLYTIGKYVCRSKEEYVDNKYIGTVRNVETIKGTNLGQLKLDYLMDGVKNFVNGEAILEGNLPLLQTWYYEKFRVHQLDSIISYESRLRPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WVG7</accession>
    <name>A0A8I6WVG7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123445136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978010.1">
        <property type="nucleotide sequence ID" value="XM_045122075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WVG7"/>
    <dbReference type="SMR" id="A0A8I6WVG7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307480"/>
    </dbReference>
    <dbReference type="GeneID" id="123445136"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0256910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0256910.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0256910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445136"/>
    <dbReference type="OMA" id="SQMIGGF"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000345">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF484">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="346"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307480.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58302" checksum="713B967EF82C0266" modified="2022-05-25" version="1">MASRAKLLHAHYLVAGLRDPDKWTCLVKEYASNAFLRDAALVYAKHLLRQTHHLPLLPVLLKAAGSRAEPGLGRSLHAEAIKSAFDGELLVGTTLVSMYCKCGLLADARRVFDGMPGRNVVTCNAMLAGYAAAGDTRRAEALFGSMGSRTSVTWATIIRVFAEKGDMDGARRWFDATPMGMRTVVTWTVLVHGYVAAGDMETARELFDAMPARNVFVWSSMVTGYFKAGDTDEARAMFDRVPVRNLVSWNALIAGYAQIGRSQEALHAFHSMLEDRIKPDEFTMASVLSACAQLGSLKQGKKVHDFLDRKRIRKNHFVLNGLVDMYAKCGDLEYARKVFDGIQWRNTECWNSMISALASHGRSEEAIRLFSQMECSEQKPNGVTLLAVLGACTHRGFVEEGLRIFNSLGVYGVAAGEEHYGCLVDLLGRAGRLEEAHAVVKNMAMEPNEVIWGALLGACRVHDNGEMSERVSNEINQLRSDGASTNDADHIMLSNILAASERWEQAERVRRKMANHGVEKTPGCSSVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYV1</accession>
    <name>A0A8I6WYV1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123442714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974769.1">
        <property type="nucleotide sequence ID" value="XM_045118834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYV1"/>
    <dbReference type="SMR" id="A0A8I6WYV1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0231410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0231410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0231410"/>
    </dbReference>
    <dbReference type="GeneID" id="123442714"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0231410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0231410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0231410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442714"/>
    <dbReference type="OrthoDB" id="674669at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd23431">
        <property type="entry name" value="beta-trefoil_Ricin_AtEULS3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040249">
        <property type="entry name" value="Ricin_B-like_lectin_EULS3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31257">
        <property type="entry name" value="RICIN B-LIKE LECTIN EULS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31257:SF2">
        <property type="entry name" value="RICIN B-LIKE LECTIN EULS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0231410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="378" mass="41195" checksum="70B6CCC06D838E54" modified="2022-05-25" version="1">MYSGDATYLGSDRIHLHHPPAFPFPESRPPPPTGQSIMEFPHRHGHGHGRRDDDDDDRRPPAPYGRQEPDPYGAPPPSYGRRPDDVAYGAPPPAYGGGREDDYGGRAPAYGGGREDDYGGRAPAYGGGREDDYGGRAPAYGGGREDDYGRHAPAPAGYGGGDYGRHAPAPAYGGGRDEGYGAPAHGNVVHVSHESGDERPQYGGYGNETRPHHGGGGGMAPPATRQPTYRVFCKAGEESFSLAARDGKVCLVRTDRDDDTQHWIKDMKYSTRVKDEEGYPAMALVNKASGEALKHSLGQSHPVLLTRYNPDTLDESVLWTESRDVGAGYRCIRMVNNIYLNFDALHGDKDHGGVRDGTTLILWEWTEGDNQRWKIVAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0T5</accession>
    <name>A0A8I6X0T5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in sporophytic self-incompatibility system (the inability of flowering plants to achieve self-fertilization).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0T5"/>
    <dbReference type="SMR" id="A0A8I6X0T5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0102640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0102640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0102640"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
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    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035446">
        <property type="entry name" value="SLSG/EP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002686">
        <property type="entry name" value="SLG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035163553" description="non-specific serine/threonine protein kinase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="10">
        <location>
            <begin position="28"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="11">
        <location>
            <begin position="343"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0102640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="444" mass="48704" checksum="943CA306369118AE" modified="2022-05-25" version="1">MSQSRHHLFLALPSVAMLLLTASAVAANNTLKRRSAIGGDQTLVSPGNIFQLGLFSVANNTKWFLGIWFTVSTEAVIWVANRDRPLNASSSGFLSLSGQGDLVLLDAASNNETVWSSNSSSSATAVAQLDDTGNLVLADDAGSVLWQSFEHPTNTFVPDMRIGKSIKTGDEWSLSSWRDGDDPSAGDFRFVMDTQGSPEFHAWSRKQGRKTYRTGPWNGVRFSGIPEMGTYKGMFEFQFTDTAHEVSYMYHAYPGSPPSRVVLDESGVVHRMVWDRTLGKWSVFWSGPRDQCDTYGMCGAFGVCNVVDAVVCDCIRGFRQRSPAEWRMRNPSGGCVRSTPLQCERGGEDGFYALRGVKLPETHGSSVDNGTSLEECRRRCLANCSCTAYAASDIRGGGSGCIQWFGELMDTRFLNGGQDLFVRLAMSDLDISAIPSQLTLQYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X406</accession>
    <name>A0A8I6X406_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X406"/>
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398330"/>
    </dbReference>
    <dbReference type="GeneID" id="123446865"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0398330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0398330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446865"/>
    <dbReference type="OrthoDB" id="603303at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="OS04G0440200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0398330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="477" mass="53224" checksum="1D81CF84F99D10C0" modified="2022-05-25" version="1">METWGADLGGRLLAESNLLCRPVADLKYMDAAGVDRLSDLTDCLLHAILSRLKARQVVQTCVLSSRWRRLWLAVPCLDIDGGEDQVGEDEERHNKLDDFVDNLLLRRSTCASSPLQTLRVSIASPRPLRMRGLHERRYSVCDAHTRWVRRGLKSSPAVLEVRGVKLPSLSSGAHRITRLLLDDVILHGDFEKHLFSRLTLLQDLAIRNTDMSEISRIESNTLKNLTVEAGNILNFEVIAPRLASLHLAVQFRGLRDFSVTVQEAPNLVHASVRLLDNPLPAKGYPFVFQQDASLLLPMLCRFLASLSNVRSLELSGFSDMAQHTSPPPSPGVPWPAGYQFELMVPGSDKSAPCPTLQAILDEEHNGLPMFRNLRTLVLEQCEIGDNIQTLCSFLHNTPALEKLTLKNCECQKVPSSSASNILEMGFMTSSLSLVQIQYDDRDDHEGQGTRQVDNVVSMVEKNMPVTAMIHVTKVRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X468</accession>
    <name>A0A8I6X468_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123425672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965309.1">
        <property type="nucleotide sequence ID" value="XM_045109374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X468"/>
    <dbReference type="SMR" id="A0A8I6X468"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134440"/>
    </dbReference>
    <dbReference type="GeneID" id="123425672"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0110760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0110760.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0110760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0134440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134440.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425672"/>
    <dbReference type="OrthoDB" id="2498029at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000036">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="62"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134440.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="330" mass="36139" checksum="2E94A451ADAF33AF" modified="2022-05-25" version="1">MTQEEAPAPVNFWGEHPATESEYYAAHGAEGESSYFTTPDDAGARRLFTRSWRPAGGGAGARPRALVFMVHGYGNDISWTFQATAVFLARSGFACFAADLPGHGRSHGLQAFVPDLEVAVADLLAFFRSVRRREEHAGLPCFLFGESMGGAICLLIHLRTSPEEWAGAVLVAPMCRISDRIRPRWPVPEILTLVSRFAPTLPIVPTADLIEKSVKVPAKRLIAARNPMRYNGRPRLGTVMELLRATDELGARLGEITIPFLVVHGSADEVTDPAVSRALHEAAASEDKTIKMYDGMLHSMLFGEPEENIERVRGDILAWLSERCTPTSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4F7</accession>
    <name>A0A8I6X4F7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123425749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965400.1">
        <property type="nucleotide sequence ID" value="XM_045109465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4F7"/>
    <dbReference type="SMR" id="A0A8I6X4F7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0136770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0136770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0136770"/>
    </dbReference>
    <dbReference type="GeneID" id="123425749"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0112750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0112750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0112750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0136770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0136770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0136770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425749"/>
    <dbReference type="OMA" id="EVEYHDA"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044162">
        <property type="entry name" value="PHOS32/34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31966">
        <property type="entry name" value="OS01G0783500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31966:SF13">
        <property type="entry name" value="OS07G0551400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
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    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="57"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0136770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="27260" checksum="1964CB011568B8C4" modified="2022-05-25" version="1">MQAPANPPVELPPLVAPPPRVKAPTPRPPPPASLQPDSPGVFFTNAAAAAPLGSAHRRIAIAVDLSDESAYAVSWAVANYLRPGDAVILLHVRSTNVLYGADWGSVTPTSPEDDAEVAARKMEEDFDALTASKADDLAKPLEEAKIPYKIHIVKDHDMKERLCLEVERLGLSAVIMGSKGFGAARRASKGRLGSVSDYCVHHCICPVVVVRTPDDAVAEGGESATAMEAAVGAEDVLHPVPEEDAEYHDAAEEHKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4Q3</accession>
    <name>A0A8I6X4Q3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4Q3"/>
    <dbReference type="SMR" id="A0A8I6X4Q3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0299360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0299360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0299360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0249600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0249600.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0249600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0299360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0299360.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0299360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035170508" description="Subtilisin-like protease" evidence="12">
        <location>
            <begin position="26"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="14">
        <location>
            <begin position="29"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="13">
        <location>
            <begin position="140"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="15">
        <location>
            <begin position="663"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0299360.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="79662" checksum="A033F4624109DFFC" modified="2022-05-25" version="1">MASLLLLASSSWLLLCSLLPAPVVADRTSYIVHMDKSAMPTQHCDHREWYSAVLASLAAAADSPGAASGDQPRLVYTYDEALHGFAATLSAPELHELSRAPGFVSAYPDRRSDVTHDTTHSTEFLRLSPFGGLWPAAKLGEGVIIGMIDTGVWPESASFDDAGMAPVPSRWRGMCEPGVAFPASLCNRKLIGARYFNRGLVAANPGIKISMNSTRDTIGHGTHTSSTAGGSPVPCASFFGYGLGTARGVAPRAHVAMYKVIWDEGRYASDVLAGMDAAIADGVDVISISMGFDGVPLYEDPVAIAAFAAMEHGILVSASAGNAGPRPGTLHNGIPWLLTVAAGTVDRTMFSGTVSYGNTTLTTITGFTMYPANAWVVGTKLVYDDTISACNSSVSMANATTSIVVCRDIGSVTEQLNTANEAGVAGAIFIRDVLTYDDTMPLPAIFIRSKDAPALLSYINSTMNPTATMRFQQTILGTRPAPVVAAYSSRGPSKSYPGVLKPDILAPGDSILASWAPVSPLGLIGQTSLASDFLVASGTSMACPHASGVAALLRAAHPDWSPAMIKSAMMTTATTDDNTFHPITDAGAGAGFDGNDDNGTAASPLAMGSGQVSPNSAMDPGLVYDAGPKDFVELLCSTNYTNAQIAAITRSSTAYNCSYSSNDVNYPSFVAAFGFNATGGETRFSRTVTSVGAGPATYHASWVSPSNVAVAVTPATLEFSGAGHKVSFEVDIKLTAPAPGGKAAYGAVVWTDASGKYRVRTPYVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X732</accession>
    <name>A0A8I6X732_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123427788</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the exonuclease superfamily. TREX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967841.1">
        <property type="nucleotide sequence ID" value="XM_045111906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X732"/>
    <dbReference type="SMR" id="A0A8I6X732"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194760"/>
    </dbReference>
    <dbReference type="GeneID" id="123427788"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0161580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0161580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0161580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427788"/>
    <dbReference type="OrthoDB" id="10250935at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06127">
        <property type="entry name" value="DEDDh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040393">
        <property type="entry name" value="TREX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13058:SF26">
        <property type="entry name" value="OS04G0623400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13058">
        <property type="entry name" value="THREE PRIME REPAIR EXONUCLEASE 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="83"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="294" mass="33422" checksum="8F0912A14FAEADAB" modified="2022-05-25" version="1">MALLFRFSQLRNSIWSSCPARLRMQHTVLSSGTQLDPKSYEKRLFSTRVQETASLHKTDLGPCISGIQPLKFKQTSEHEQSAPLLIFDIETTGFFQKKTTGNFQKDNRITEFAVRDLCGGKNSTFETLINPERGVPGYLKTVNDINTELVCKPDVPRFSDVLPLLLAFVRSRQTPGKPVIWVAHKVNTFDGPFLAQEFNRCSAQMPEDWLFVDSFCLARKLPKVEASEDKKHLLNLESLSKRYDISVKGSAHRAMHDVTTLCHVFQKMSVDLKLTYEGLINETTKASYFNNLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XBD0</accession>
    <name>A0A8I6XBD0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XBD0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0315430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0315430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0315430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0263500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0263500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0263500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0315430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0315430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0315430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0315430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="138" mass="14146" checksum="75E47C2758C34F0F" modified="2022-05-25" version="1">MHNHYFSPYLYKYFFTSNCSINSGFLLIELGSCSLPFSSSLASFYLIGAAVYLGEMDPHGPGGGPGSGGHVGWGPGGWSLVPPGGPGFPGDPGPGSWGPGFGGFFGSCIYLLCCCCLLQDCCGPLFGHPGPGGTPPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDH6</accession>
    <name>A0A8I6XDH6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123425505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDH6"/>
    <dbReference type="SMR" id="A0A8I6XDH6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0120250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0120250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0120250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0120250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0120250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0120250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF28">
        <property type="entry name" value="OS02G0603500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="3">
        <location>
            <begin position="164"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0120250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="341" mass="39485" checksum="0DBA5E2AE919B7E0" modified="2022-05-25" version="1">MCVFVDCLQMDDDESFLDTMGFGYTQTQLESPIGEQPTPSTQHQATPITEKAKSNKGKNWSSEEDNILIAAWGNTRLDSVGTDQNRDAYWDRISEYYNSHKMSSWSERNANVINCRYTLINKETARFCGCLQQILNREESRRTIEEKTNDAHIMFKEMDLKKKKPFTLMHCYVELSKYPKWQTKELETSRKKQKKTVGASPGTSTNDLADASSVHTNATSIHRDALEHEQRPDGVKKDKLRKGKADDSACKLSLETVWAAKQEKDDIKEAAKNARYAHQFELRKEEIALKKKEDARNEMEDARRQFELDERVMLIDTSGMTDMQKQFYQAKQNEILARRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XDT7</accession>
    <name>A0A8I6XDT7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123446736</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979222.1">
        <property type="nucleotide sequence ID" value="XM_045123287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XDT7"/>
    <dbReference type="SMR" id="A0A8I6XDT7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0393730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0393730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0393730"/>
    </dbReference>
    <dbReference type="GeneID" id="123446736"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0327530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0327530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0327530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0393730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0393730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0393730"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446736"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0393730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="573" mass="62541" checksum="608B9DED4A353A6A" modified="2022-05-25" version="1">MASPKENLQLEEPLLEDSVLKRRDQIVDIKGTPAPGAKHRNGSWKACKFILVTECFEELAYYGIQFNLVTFLKTILQESNVSAARNYTNWQGTCYIAPLVGAIVADSYLGRYLTTLAFFAVYLIGMAAMSVSASFPAACTGLDCLQDVGSSPSSQSAVFFLGLYMMAIGAGGIKPCVSSFGADQFDDSVPAERLKKSSFFNWFFFSIYIGSFVSGTVVVWVQDHCGWVVGLWIPTLFIALAIASFLLGSGSYRVQKPLGSPLARVSQVVVAAVRKRNVRLPRDASLLYDRSEVESVVEGTKKLQHTPVLSFLDKAAVISSAKELYSNPWRLCTVTQVEELKIVIGMLPIWATGIVYFSVLAQFSSTFLEQGRMMNTVVGTFAIPPASLASFDAVSVILFVPVYDRVLIPAARRFTGNERGLSELQRFGVGLFLSVLVMAAAAVVETRRLALDRMAAAPMCILWQVPQYLLVGASVVFACVGQSEFFYNEAPESMRSLCSALGLLTVSLGSYLSSLVVSVVSGVTTRGGEAGWIPDNLNEGHLDRFFWLIAALSTLNLAVFVWCAKRYKCKDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFW9</accession>
    <name>A0A8I6XFW9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFW9"/>
    <dbReference type="SMR" id="A0A8I6XFW9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0185650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0185650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0185650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0185650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0185650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="235" mass="26902" checksum="C15F77FE1BD1F4A3" modified="2022-05-25" version="1">MATFVRVKFTEFCQPDATMPFGKELLQITKDLRIALPTITGKPTSSYPEDSRYRIEVHVPGRTFQPRTEPVDFKFIAPNWILGRDMAIHCALGGIKEEYKCCPISPDLFTISRRNENGEIISSKTKDALLSYAQTLEKHSGTLEHRVIKDARKIKQLSLRELELEEAAREAHYEHELEAKDFLERIEKLKARVAYLEEELDMGENLHPEGDVAPLISNDEDYEESSTEGPTTMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGK7</accession>
    <name>A0A8I6XGK7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123447474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980015.1">
        <property type="nucleotide sequence ID" value="XM_045124080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XGK7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414380"/>
    </dbReference>
    <dbReference type="GeneID" id="123447474"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447474"/>
    <dbReference type="OrthoDB" id="1922547at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304:SF71">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="269" mass="28002" checksum="A819E14DD7FA6A54" modified="2022-05-25" version="1">MRASCNGCRVLRKGCADDCTIRPCLAWMRSPDAQANATVFLAKFYGRAGLINLLAAAPDDAQRPALFRSLLYEACGRATNPVYGASGLFSTGNWEACQAAVQAVLEGRRIPQVGADQAAPHPGLTAAYGVRRIPKDTIDRAPAALRVSRAGRTSFKRASPSSSAAGRNGSDCSHDHVVEDDGKAPEDMHKRGESSSSEAEAGSHVSQAAQSASATVPQVAQDDGEIGLELTLGFGPATRVLRSPPPPPPPPPAARAMSGHIGLLLGLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIJ0</accession>
    <name>A0A8I6XIJ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123428462</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044968608.1">
        <property type="nucleotide sequence ID" value="XM_045112673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XIJ0"/>
    <dbReference type="SMR" id="A0A8I6XIJ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213640"/>
    </dbReference>
    <dbReference type="GeneID" id="123428462"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0177670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0177670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0177670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0213640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0213640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0213640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428462"/>
    <dbReference type="OrthoDB" id="276276at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03671">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
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    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022927">
        <property type="entry name" value="RppH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001936">
        <property type="entry name" value="PRK00714.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001938">
        <property type="entry name" value="PRK00714.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF22">
        <property type="entry name" value="NUDIX HYDROLASE 26, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
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            <begin position="56"/>
            <end position="204"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0213640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="218" mass="23926" checksum="8B16C7847348A136" modified="2022-05-25" version="1">MTVAAARCLILTPTISSSAAAAAAVLRLPRIARRRPLSCSASPLAVFASMDSPPEGYRTNVGICLADPSLTKIFSASRIDIPTAWQMPQGGIDPGEEPRAAAIRELREETGVRSAEIVAEAPNWLTYDFPADVKDKLNARWGTNWKGQAQKWFLFRLTGGDDEINLMGDGSEKPEFSEWAWMTPKQVIEKAVDFKKPVYEETLKHFAPYLQSDPAASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XJ16</accession>
    <name>A0A8I6XJ16_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123450637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044983723.1">
        <property type="nucleotide sequence ID" value="XM_045127788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XJ16"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0388810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0388810"/>
    </dbReference>
    <dbReference type="GeneID" id="123450637"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0323450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0323450.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0323450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0388810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0388810.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0388810"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450637"/>
    <dbReference type="OMA" id="PVWEADQ"/>
    <dbReference type="OrthoDB" id="773117at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040381">
        <property type="entry name" value="At4g14450-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33912:SF5">
        <property type="entry name" value="F22G5.17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33912">
        <property type="entry name" value="OS01G0939400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0388810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="203" mass="21092" checksum="38B3B9DF9064A817" modified="2022-05-25" version="1">MRASRDCRERKKSAIAGQLSPRTRRSMEMKLSPGTGEVFGGKKRPPSRLQKKAPASLQLEQAGPGAAQPSAAAWGDGRTPIPLLSPLVTSPAPAWEADQAGSRREAAQAETRSGGDADGILSPIRRGGYGVSNGADETATTPALSGGWRHPAMPAPTPTLASNGGGVWRHPAMPSPVPEPASLAPLFKSQCAVELHNPQAQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XPB9</accession>
    <name>A0A8I6XPB9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Hordeum</taxon>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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            <begin position="113"/>
            <end position="125"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="220" mass="24347" checksum="A2631EAB14E5086C" modified="2022-05-25" version="1">MFLAPPVDAAPINAVPLSAVAPRAEPAWIRSHFGLRLDLTVTFIDEKVVTATDLDLQKNQFRLPTAGVLYSLRPLLSPEELDAASIPRDGAKVVPRLSRSPPIEEEEVQQGSIKKRKKKQGKKHGGGLPVVVCNVHAGTKQLQLTRWESSHGNIIKGGGYLDFITRCCFKEKDIVEIWAFKERPFHLFGVDMCHASPLYVVLAKKEQRQPASIGMGKCTM</sequence>
</entry>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075">
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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            <end position="162"/>
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            <dbReference type="Pfam" id="PF13456"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0419570.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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    <organism evidence="2 3">
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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                <person name="Muehlbauer G.J."/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0492150.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="122" mass="14260" checksum="DC5FFCED55C2CDF2" modified="2022-05-25" version="1">MEALLHMPYHLIIPMNLLEWLVQHIFGNVEPYFKHKNKVIKITKDMVDKIFDFPGGTVPFVFSSDDPQVKSEVSELRSKYVDHRNKMPINKIEEVMLSDETEDGFIRSFTFYFVSTIMCPAS</sequence>
</entry>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="4" key="1">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0425490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0425490"/>
    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0425490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0425490.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 5H"/>
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    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
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        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS04G0409100 PROTEIN"/>
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    </dbReference>
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        <location>
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            <end position="195"/>
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        <location>
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        </location>
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            <end position="257"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="291"/>
        </location>
    </feature>
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        <source>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0425490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="298" mass="32444" checksum="E453966CA6EBDDDD" modified="2022-05-25" version="1">MRFCDAWLATPLRLDDLELPVPACGKVSSIQPWTARADVVTHALDSHPSPSRSSASPALPDLLPRPRRRGRGRQLAAKHARKVSLFFSPEWCHDALADPLLGCPTLQVLALGKCHLSDARASAVAAAALTELTLLVLLGVWHYKQVDEITVEDAPCLERLLGNMRLNAAITVTGAPKLTAFGYAVVTIPHLFHGERAPSQARRTWRRSGADEYYTTVSDYIQQRYPIRAHVLQFMRIQSKIAPCGKQEPARVHRQRNDASAASRTGAPRFGAAQQEEGGQRNDASAASCTGTPCFGAA</sequence>
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    <accession>A0A8I6XXB6</accession>
    <name>A0A8I6XXB6_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference evidence="3" key="1">
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XXB6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0435850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0435850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0435850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0435850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0435850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0435850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0435850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="98" mass="11003" checksum="1D9599DCFF73CADE" modified="2022-05-25" version="1">MEQCRQGKSAKRIRNFGKRIGSEDWARGSRPRTRRLLADCSSCSRGESGSPRADRGTDRESPLRELSPSMKQSTQNWYGQGESDCLIKTKHCNGPRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZI5</accession>
    <name>A0A8I6XZI5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123401883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951676.1">
        <property type="nucleotide sequence ID" value="XM_045095741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZI5"/>
    <dbReference type="SMR" id="A0A8I6XZI5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0564980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0564980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0564980"/>
    </dbReference>
    <dbReference type="GeneID" id="123401883"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0468290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0468290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0468290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0564980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0564980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0564980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401883"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05289">
        <property type="entry name" value="MDR_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044626">
        <property type="entry name" value="AOR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44573">
        <property type="entry name" value="NADPH-DEPENDENT ALKENAL/ONE OXIDOREDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44573:SF1">
        <property type="entry name" value="NADPH-DEPENDENT ALKENAL_ONE OXIDOREDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="4">
        <location>
            <begin position="265"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0564980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="383" mass="39697" checksum="5A5B55C257A021B4" modified="2022-05-25" version="1">MQAFLAYSILPNPCSRSCFPRPATRPQLLLSSVPAARTSDAATARRCGGPGRSVVTAASSSPAVAVTATDVPGTMKAWAYEEYGDASVLKLDEAVSVPAVGEDQVLVKVAAAALNPVDSKRRMGKFKATDSPLPTVPGYDMAGVVVKVGSQVKNLKEGDEVYGHISEKVLEGPKQFGSLAEYTAVEEKLVALKPKSIDFAQAAGLPLAIETAHEGLERAGFSAGKSILVLGGAGGVGSLVIQLAKQVYGASKVAATASTPKLELLKSLGADVAIDYTKDSFDDMPDKYDVVFDAVGQGEKAVKVVKEGGSVVVLTGAVTPPGFRFVVTSDGSVLAKLNPYLESGKVKPVVDPKGPFPFSQVVEAFSYLETGRAAGKVVISPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0I9</accession>
    <name>A0A8I6Y0I9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123446666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979161.1">
        <property type="nucleotide sequence ID" value="XM_045123226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0I9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0389440"/>
    </dbReference>
    <dbReference type="GeneID" id="123446666"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0323980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0323980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0323980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0389440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0389440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446666"/>
    <dbReference type="OMA" id="HDIICGV"/>
    <dbReference type="OrthoDB" id="724026at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31439">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31439:SF3">
        <property type="entry name" value="OS07G0231800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="521" mass="58346" checksum="6286AD518455D827" modified="2022-05-25" version="1">MESIGPALPSLPSMASGIAPDVWQWIKSLPRQWRADEFHSLLICNSPSTNQSLNLVVTKQSEETHPFSLSFSICDKSRDHDPVSLWSSHYSKLKPANTTDVAAQFLLEIICGVLRYGPYSSSRAIFRLPAVHVSDGSGKILSLSVLALAFLVCIYEAPSTPRREFIGAIAARLTRDEMRHAARELMLALGSNLEEQWMRSLNLGVTNWAMEAHRSGAGTSSPLRFAVFSYALSASRLWKVQLYCPVVAMTMEHHPSHHHQQQQQLVKDERLLFSLNYQQLESVIQFVYRVAFHENWIDVTVNVDNIRCDVIQLVSETLMARQGHGSDEKHFPSRISLQLTPVAQSNILSLSVSRSTDNPAQEVGTDKGIDTTIAAAPASIGISVSAHETVTRTMKPWKFEHSVHGNTASLSWFLHGSGGGGREVFSSDPPKLELFQPKSWFRNRYTSPSRPFTRSGGVIFAGDEYGEGVCWRMGAAAAGKTMEWEIKGRIWVTYWPNKKRTLHTETRRLEFRELLQLTIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2S3</accession>
    <name>A0A8I6Y2S3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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            <end position="475"/>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="475" mass="55814" checksum="048875A47F77779B" modified="2022-05-25" version="1">MAMWVQLTQVLKGCMGGFGYGIRNQEGEDVLSFVLSFALACDMVVANTLFRKRESHLVTFSSGLHSSQIDFVLSRREDRRACIDCKAIPGESVVPQYKRAKVARTKWWKLKGEASQAFRERVIKEGPWKEGGDANLMWTSMATFLRKVAVEEFGVTKGSRREAKDTWWWNDEVQKVIREKKDCFRCLYLDRSAANMEKYKVAKKAAKRAVSEARGRAYEDLYQRLNTKEGERDIYKMAKIRERKTRDVGEVKCIKDGDGQLLVKDEAIKRRWREYFDNLYNGEVDSSTIELDDSFDDTSMCFMRRIQECEVKEALRRMKVGKAMCPNGIPIEVWRGLGDIVIVWLTKLFNHIFRSNKMPEEWRRSILVPIFKNKGGVQSCTNYRGIKLMSHTMKLWERVIEHRLRRLTSVTKNQFGFMPGRSTMEVIFLVRRLMERYREQKKDLHMVFIDLEKAYDKIPRNVMWWALEKHKVPIK</sequence>
</entry>
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        </submittedName>
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    </gene>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7R1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0464070.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0464070"/>
    </dbReference>
    <dbReference type="GeneID" id="123452834"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0384090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0464070.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0464070.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="311" mass="33174" checksum="8F9908CB2F3800A6" modified="2022-05-25" version="1">MDFLVCVCLLQLLFLRSSPVAAQQPARALDAMLQEYAYRALVRPRTGIVYNATVPGNLAGVSVSALRLRSGSLRRKGLSQYFQFGLPTGVIAQRRVERMVLVYHDLGNWSQHYYPPPPGHTYLSPVLGLLAYDAVNLSAVGLPELNIVASGSPILVNFSNAMAAPSGGPAPRCVWFDLDGVPQFRGPEAGNVCATYRQGHFSIVVNSSEGAPAPLPPGAIAPPIPAAGGRTKGDSQAWKVAVAVVGGAIALGLLSSVLLCLVRHKRAKKLEVMERNSEVGETLRMAQVGRAQAPVALWTRTKPVIESEYAA</sequence>
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        <recommendedName>
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    <organism evidence="2 3">
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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            <strain evidence="2">subsp. vulgare</strain>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y8Y6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0558090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0558090.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0558090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012871">
        <property type="entry name" value="DUF1668_ORYSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33085:SF126">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33085">
        <property type="entry name" value="OS12G0113100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07893">
        <property type="entry name" value="DUF1668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0558090.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="266" mass="29031" checksum="F07EB07DEFC95F0B" modified="2022-05-25" version="1">MSAPKWCPSLSLAVDGQDGAYVIEAAPQADGPGRRSVEALAYDRKPVDDDDDEDEGWVWRALAPPPYVREPGYGDDEGGRSGKITCHAAEGARIWVSTAGRGTYSLDTASDAWTKAGDWALPFRGRTVPVPEHASRLWFGVSASNAGKICASNLHAAANEGRAPAVDQVWDGFAVPEGFHDLEPHSYVVHLGDGRLCVAKFFETTRQEADDDWTCRRCCHGYVSECAFAMFTGVEVEPGAGRWHRLIRHRSLRYSLGTDRVPVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YA02</accession>
    <name>A0A8I6YA02_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123403001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044952913.1">
        <property type="nucleotide sequence ID" value="XM_045096978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YA02"/>
    <dbReference type="SMR" id="A0A8I6YA02"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549470"/>
    </dbReference>
    <dbReference type="GeneID" id="123403001"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0456610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0456610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0456610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0549470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403001"/>
    <dbReference type="OMA" id="PPHGRGF"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="CDD" id="cd01518">
        <property type="entry name" value="RHOD_YceA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00469">
        <property type="entry name" value="TrhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268:SF3">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="220"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="448" mass="49772" checksum="12217F0128738AA5" modified="2022-05-25" version="1">MLPSPPLSAARRAATAAAHHLLPFISSLTLDPPAAPRRRVRHMPLRNSAPQVSPPPHGRSFAVAAGGGGLELEAEAPALVVVSFYRFADFPDHADLRRPLKDLCEELRVSGGIILAPEGINGSLCGTREAVEKVLNFIQTDNRLKGLRVIQTPVTPEDEAIHHGHTSHSPVGPGEDAPFRWDHVRVKLKKEIVSFGDPGVMPTKRVGKYIKPKDWNSLISDPDTVVIDVRNMYEIRIGKFKRAVDPCTESFREFPSWVDNQFQLAESDSHQPSVNGNTVTEGAEEDLNSSEPKELPRVAMYCTGGIRCEKASSFLLNKGFKEVYHLEGGILKYLEEIPEAESLWEGECFVFDKRVSVEHGLAQGTHKLCYGCKQPVSDEDMESPKWEYGVSCPYCFSTRSEEEKERARARHRQFKTWGVIGGPDKGRSPKRLEASNGVKEDKQPHNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YB97</accession>
    <name>A0A8I6YB97_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YB97"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0650160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0650160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0650160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48143:SF1">
        <property type="entry name" value="ZINC FINGER GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0650160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23502" checksum="B1C5841E2F7A3594" modified="2022-05-25" version="1">MSLPCSDQSIRPKKMKSASLPRRVEAVRCWCGDLCKVKEVEDFSDWLGMKFFMCANYESDPPECISPYIRPPYPPPLCMYYRWIDTEMTDWAVTEIRQRGRRAWASWDLEERREKAEAEEKAAQKKEWEEYCVEQRAFLDEMIRKNQEEKLQQEEVYRQREQAHQAERERKRERARAAKAAEEASDGKGKHPRWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YD94</accession>
    <name>A0A8I6YD94_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YD94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431890.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519560.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519560.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="78" mass="9091" checksum="38DC3263ADF1BF5A" modified="2022-05-25" version="1">MSLQFLTLTWTQQDHTTICHALTLLCLRKSFFFYEKGPCFARDVIRVPDAGSLIAAQFFQFVHLKNQDTMISRREHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YDP3</accession>
    <name>A0A8I6YDP3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6YDP3"/>
    <dbReference type="SMR" id="A0A8I6YDP3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0365210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0365210.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0365210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0441190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0441190.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0441190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
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            <end position="308"/>
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        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="95"/>
            <end position="199"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0441190.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="33950" checksum="D10E0D5BF51FABD8" modified="2022-05-25" version="1">MKHLLLSCTCLILCAVVTPVSADIRREAGALLSWKASLAGADESLSSWSLANSTGVCHWMHISCNSARSTIIKLNISGANLNGTLDKFDFSAFPNLKKLMLFQNGLYGNIPEGIGDLTSLVWLRISSNWYITGTIPRGIGRLKQLTELQLGGLGLDGVALPEEIGNLTSLTKLTLYSVSLTGSIPLTFGRLKNLRLLNLRNNSLTGRIPLEIGNMTELHRMDLTQNYLHGQIPGTFSRLVKQNGLLLSENQLGGDVMLELGNNIVLCYIDIAKNNFSGRSTDYRHWCIQWIYEPSEPDLSELHNTEVH</sequence>
</entry>
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    <accession>A0A8I6YES2</accession>
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            <fullName evidence="5">Secreted protein</fullName>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="3" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035312696" description="Secreted protein" evidence="2">
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            <begin position="26"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11743" checksum="BA984026E485E332" modified="2022-05-25" version="1">MMYQQRATCFLLVVVFLLLLPLMASYPCVPAAHDLLRQAPDGDIGVWSAPDGMHYAGEKVLGTRTARRLGRRIPMKNPPSPIPNENSGVVLPLTPPPSSLINKQVLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YG88</accession>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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    <reference evidence="3" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0731810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044961427.1">
        <property type="nucleotide sequence ID" value="XM_045105492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKR1"/>
    <dbReference type="SMR" id="A0A8I6YKR1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722940.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722940.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722940"/>
    </dbReference>
    <dbReference type="GeneID" id="123412545"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0599500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0599500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0599500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0722940.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0722940.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0722940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412545"/>
    <dbReference type="OrthoDB" id="504868at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052684">
        <property type="term" value="F:L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000025">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009057">
        <property type="entry name" value="PRK12391.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="116"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0722940.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="491" mass="53340" checksum="66A9C6753D091A03" modified="2022-05-25" version="1">MATASTALRPPRLPAVAEQASPLHRLPKNRVVAVNVRRSFAARAGSYPGNVGVPKQWYNLIADLPVKPPPMLHPGTHQPLNPSDLAPLFPDELIRQELTEERFIDIPDEVRDVYELWRPTPLIRAKRLEKLLGTPAKIYYKYEGTSPAGSHKGNTAVPQAWYNAAAGVKNVVTETGAGQWGSALSFASTLFGLNCEVWQVRASYDQKPYRRLMMETWGAKVHPSPSDVTEAGRRLLAADPSSPGSLGMAISEAVEVAATNADTKYCLGSVLNHVLLHQTVIGEECLEQLAAIGDTPDVVIGCTGGGSNFGGLAFPFMREKLAGRMNPQFKAVEPAACPTLTKGVYTYDYGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGFMEAMSIQQTECFEAAIQFARTEGIIPAPEPTHAIAAAIREALECKRTGEEKVILIAMCGHGHFDLAAYDRYLRGDMIDLSHSSEKLKESLGAIPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YKY2</accession>
    <name>A0A8I6YKY2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123398861</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the defense reaction of plants against pathogens.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949242.1">
        <property type="nucleotide sequence ID" value="XM_045093307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YKY2"/>
    <dbReference type="SMR" id="A0A8I6YKY2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519310"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519320"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519330"/>
    </dbReference>
    <dbReference type="GeneID" id="123398861"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431630.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431640.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431670.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0431680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0431680.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0431680"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519310.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0519330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0519330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0519330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398858"/>
    <dbReference type="KEGG" id="hvg:123398860"/>
    <dbReference type="KEGG" id="hvg:123398861"/>
    <dbReference type="OMA" id="WSTRNHT"/>
    <dbReference type="OrthoDB" id="745551at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044692128" description="SCP domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="25"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0519320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="17869" checksum="A3296D710856E824" modified="2022-05-25" version="1">MEYSPKLAVLLLLALVSAMAVTAQNSPDDFVDAHNAARAEVGLGKVTWNATVAAYAQDYAEQRRGDCQLIHGVNRPYGENLYGGDGFGTTWTAANAVSSWVSEKQYYDHGSNSCSAPADKSCMHYTQVVWRNSTAIGCARVICASGNGVFIICSYSPPGNYPGVSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YMX2</accession>
    <name>A0A8I6YMX2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YMX2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0737840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0737840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0737840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0737840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0737840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0737840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF43">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="302"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0737840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="567" mass="60933" checksum="A3F48E5E06B2EAD9" modified="2022-05-25" version="1">MDAHTGTPLTLLYSAAPPPPPPAAENPSAGSVGGATAEAPPTAGLTRSPFLPPRTTAMGVVAPAPSRAAAAPSKLLNAARPKKEKTSGKKNKAADGSDSSKGKNLAGRRTGAAATEAPASSLVEPAAAAHQVFDEMPPSLNDDAYMSTMGVGSNNSHWSQTNDIHIEDHEFEVDEEGEGIVNAPKGRAGNYTTNDDKLLCNTWLQVSRDPSIGGDQSRDAYWGRMKEYFDAQNVSGIDRSERSLRSRWSTINSDCQKWAAAQKAVDKINPSGTNEDDRYNIAQNLFKEETRTTKKGKIKKGKIFTFPHCYEVLKDDEKWKKREDLDDLHLSNKRKRTIELNDDDEEDDASSDDGKRSPTPNSVSYSKPKRPDGCKKDKTEKKKRKGDDELTNAMEAIVKARKEANEVRKMARNQDAAAEERRLAVEERRVVAEERKVALQERKVGMEERAKLLEWEKHLFFLDTSLFNDAQKEYVNLAREKVLIQKRAMIRGMGGGGLGAMGGGGLGAMGGMGGFGAMGSMGAPPAAMGGMGGFGAPSDAMAAMGGMSFASIMGGMGAPPAAMGGMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YQS9</accession>
    <name>A0A8I6YQS9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123399898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044950217.1">
        <property type="nucleotide sequence ID" value="XM_045094282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YQS9"/>
    <dbReference type="SMR" id="A0A8I6YQS9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0465240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0465240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0465240"/>
    </dbReference>
    <dbReference type="GeneID" id="123399898"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0385030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0385030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0385030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0465240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0465240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0465240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399898"/>
    <dbReference type="OrthoDB" id="6358435at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023238">
        <property type="entry name" value="FAM175"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31728">
        <property type="entry name" value="ABRAXAS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31728:SF5">
        <property type="entry name" value="OS07G0540200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02051">
        <property type="entry name" value="PROTEINF175"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0465240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="281" mass="29849" checksum="6CE83216251E9E38" modified="2022-05-25" version="1">MAVGEEVKLSISGAALAALLHRCGAAAGDCDGLLFGRAHRPPPPAPTLSDYDDQAPAAPCLFISISGHSSLSHPCSLSDPLGRPHPPPPSSPCPLGFFSSRRRAPLRASMRELALACSLSKTLSPLAHPLLVILVSPNTSDDLSTHSFDYRAFLLLGSRFVPTSFAVVNVGPGFRDQYHSFAPESSFPLLTQPSPPAAPEAEDAYSIGEQKKVDAMVDGFGLGRLQELVGAVAGQAAGMDSMYAGMLQRMEKLAREVEKSNHRVLDQEKRNLVLRSKTAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YRB1</accession>
    <name>A0A8I6YRB1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YRB1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0567470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0567470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0567470"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0567470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0567470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0567470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF187">
        <property type="entry name" value="MUTATOR TRANSPOSASE MUDRA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01007">
        <property type="entry name" value="TRANSPOSASE_MUTATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="MULE transposase" evidence="4">
        <location>
            <begin position="151"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0567470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="471" mass="54218" checksum="A5214306EE35EDEB" modified="2022-05-25" version="1">MVCSSQIKEEKTHCIRQIMLPHTCGTTTDTSRINSTWLAKEYEDEIRSDPSMSITALIDAVQRRFGIEISRHMAYRAKNKALEVVEGDGDRQYLCIRDYLQTVMEKNPGSRCHVWTIRPINKPEKNPRFHGLFFGLHAQIEGFKNGCRPFIGIDGCFLKLGNGAQVLAATARDGNNNFYPLVFGVVGTEDTATWSWFLNQLKYALGGTTGQFGTYTFMSDRQKGLLAAVHLVFPHSSHRYCLRHIYANFQSAGYKGGDLKKLVDAAAYAFDKCDFDVAMEELRKEDKDAWEWMCNIPPKHWARHALDTHCKTDLVVNNISEVFNSYILKVRDKPIVTMIDSIRTKLMPRYASRREGVEHVQWEITPTYAERLEWEKRNARWCTAVCAKKGLWQVTNTPRTYEVNLTESSGCFKWDLTGIPCKHDVCAIYKAKEFPEDYVSDFFKKPMYKEAYQKPHISSSRSPWMDENENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YS54</accession>
    <name>A0A8I6YS54_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401138</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044950802.1">
        <property type="nucleotide sequence ID" value="XM_045094867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YS54"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0576480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0576480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0576480"/>
    </dbReference>
    <dbReference type="GeneID" id="123401138"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0477970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0477970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0477970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0576480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0576480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0576480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401138"/>
    <dbReference type="OrthoDB" id="670255at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF485">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="143"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0576480.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="287" mass="31678" checksum="4BA255AE7D1194D9" modified="2022-05-25" version="1">MEPNHHGYGYGYYDSNDGSYTNDDNPCYYGMSYATSEPSSYGYQYQEPQSYDNHYHHQYDMTNSSSSSAAPSYAAYDDIAPYAQQQHPQSLNFGGGSYQYDGAASAMGMDQFSALMEATSISPAAPCWVEQQEAMKAKAEPPQLIGVRRRPWGKYAAEIRDSRRSGERVWLGTFDTPEAAALAYDQAAYTSRGTAAVLNFPVERVRESLCVLKLGKAAAAGEDDSPVLALKRRHCIRKRTPKSKGQEGDMKKKQPAAASASSVLELEDLGADYLEELLALSEQWSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YSB0</accession>
    <name>A0A8I6YSB0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectate lyase</fullName>
            <ecNumber evidence="4 10">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123403422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 10"/>
    <dbReference type="RefSeq" id="XP_044953294.1">
        <property type="nucleotide sequence ID" value="XM_045097359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YSB0"/>
    <dbReference type="SMR" id="A0A8I6YSB0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0578880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0578880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0578880"/>
    </dbReference>
    <dbReference type="GeneID" id="123403422"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0479990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0479990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0479990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0578880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0578880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0578880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403422"/>
    <dbReference type="OrthoDB" id="1637350at2759"/>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00824"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007524">
        <property type="entry name" value="Pec_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF208">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04431">
        <property type="entry name" value="Pec_lyase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035337413" description="Pectate lyase" evidence="10">
        <location>
            <begin position="30"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="12">
        <location>
            <begin position="151"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0578880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="425" mass="47066" checksum="399104FECD990F20" modified="2022-05-25" version="1">MDRGLQWSRPASLLLLGAAFLATAAVASANPAYNPDPYSVVDNFNRAVHRSTSPRRALSSEKKEKKQKYNGPCLATNPIDRCWRCRKDWATDRQRLARCAKGFGRGATGGLHGKIYIVTDPSDEDFTNPRPGTLRWGVVQLEPLWIIFARDMIINPTQEIIIQSNKTLDGRGAQVHIANGGGLTIQHQNNVIIHNLHVHDIKHTDGGNVSLAANHATIRTRADGDGVSIFNATNVWVDHLSMALCEDGMIDVVAASTAITISNCHLTNHNDVMLFGADDNKPEDKVMQVTVAFNHFGRGLVQRMPRCRYGFFHVVNNDYTHWLMYAIGGSSEPTIISQGNRYIAPPNMAAKQVTKRDYAPEAVWKNWVWRSEGDLMMNNAFFQTSGGQISQKLNGKDLIKPKPGEYVTRLTRFSGTLACKPGCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVY9</accession>
    <name>A0A8I6YVY9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVY9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0620840.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0515030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0515030.1.CDS.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0620840.1">
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    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF11">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0620840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="181" mass="20413" checksum="54A479F973F76E1E" modified="2022-05-25" version="1">MPGDFSQWIMRYYDPEMLQIVIPERGKIPADAASVSRIWGLPNRGRKVCFDNCPEITKAMYNIYNITSKNSSSLTNWCKMIKEMNGSHDDDFVRAWLALAFSSFLAPSTSLSISPKSFSVVMDVNVITETNICQFVVDQLKLAFTLGRRNKKAVCCCVFHIVVSASSLFVASIHFFFSNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YWG2</accession>
    <name>A0A8I6YWG2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I6YWG2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0704580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0704580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0704580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0704580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="119" mass="13878" checksum="3B09F550A3ADE9B8" modified="2022-05-25" version="1">MVLISVSVVPCPVYDSDVEVCESEIPTDPIVERLNLVEKENKYLKEKLKIREEEKMKLELYVADVVDDHKIKMEKMRLKIRKIRKYAIDSEAWYHYVVVSIVTLVAILIAFVVAFKCFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YX57</accession>
    <name>A0A8I6YX57_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123404209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044954061.1">
        <property type="nucleotide sequence ID" value="XM_045098126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YX57"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0630390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0630390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0630390"/>
    </dbReference>
    <dbReference type="GeneID" id="123404209"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0523350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0523350.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0523350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0630390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0630390.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0630390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404209"/>
    <dbReference type="OrthoDB" id="600273at2759"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF93">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="118"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0630390.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="412" mass="45872" checksum="1832023F8BF69AEA" modified="2022-05-25" version="1">MADWAPMAAPAPPPAKRKKEDPNRLQTWPLLPSTAAAGWSGLPPDLLRRVADSLLATNDLDCYMHLRAACPGWRAATEGPKAKDSSGSDRRFEPRRWILLDEVFRSEGEEMLLVNADTGRFLRRRFPMLRDHYAVATTPSGYFVLADKSPPHAASVLNPLTGAVVRFRVPVPPQMGAADVVSGDDGSPLKLVFICNSSHERHYTADPDSRSFTVHERTSDYNFLLKAAVGGGVRESLNQAGMSPILMSYLRAYSFPPGLSLSMLLPNVFSGCTYAGDDDRGFLVGLDGHMLLVFRKRTAVYPSVLRLDAATRRLEPMRSISRFAIFAGHRRCVAVDAHKLPGVEPNCVYFTRHLNRSTHIWKYNFGFVKAISQDIDFVERDDQFVLVADRPFTIVHLLSSYTINMPDSQLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z0J5</accession>
    <name>A0A8I6Z0J5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aromatic-L-amino-acid decarboxylase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0J5"/>
    <dbReference type="SMR" id="A0A8I6Z0J5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0534330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0534330.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0534330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0643080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0643080.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0643080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF171">
        <property type="entry name" value="AROMATIC-L-AMINO-ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0643080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="25643" checksum="7CB53C15497BD19F" modified="2022-05-25" version="1">MGSLDANPMSFSAFPGDKAAFEPLNPQDVRAYLHKAVDFISDYYTNVESMPVLPNVKPGYLQDELSASPPTYSAPFDVTMKELRTSVVPGMTHWASPNFFAFFPSTNSAAAIAGDLIASAMNTVGFTWQASPAATEMEVLALDWLAQLLRLPTTFMNRTSTGRGTGGGVILGTTSEAMLVTLVAARDAALRRSGSIGVSHLPRLAVYAADQTHSTFFKACRLAGFDPANIRSIPTGPETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z4P3</accession>
    <name>A0A8I6Z4P3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z4P3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0690820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0690820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0690820"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0690820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0690820.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0690820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0690820.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="22554" checksum="D9D3272994887E6A" modified="2022-05-25" version="1">MRYKEMAASKGKPFPFKHAWAILQTFDKWKLRDQETAPKKSVMLRMDYSEDEEERNLGKPEGTKKGKLRVKMEGEASSLREKMGHMMKAREELTTKTLETKLLITEKKKEAKLAQVEAKREEAKRKADLEERMINIKEAKVWKELMVEEKEHMMMSKKDMDQDQLQWWKDYKEDIAERKRMFRGASSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z7E0</accession>
    <name>A0A8I6Z7E0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123413474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962348.1">
        <property type="nucleotide sequence ID" value="XM_045106413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z7E0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655440"/>
    </dbReference>
    <dbReference type="GeneID" id="123413474"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0655440.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655440.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655440"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413474"/>
    <dbReference type="OrthoDB" id="603213at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040361">
        <property type="entry name" value="TPD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33184:SF35">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33184">
        <property type="entry name" value="PROTEIN TAPETUM DETERMINANT 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24068">
        <property type="entry name" value="TPD1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035328933" evidence="2">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="101" mass="10613" checksum="27A342A90DBE2D07" modified="2022-05-25" version="1">MEIKPALLLVACLLLGISNRGTAATCRTSDIVVQTESWPDPAGTPVYYVNLVNNCGCTQKNVKLACPGFNSSVEVYPAAAIKPDGDGKLCSLYSGAPVRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZCG4</accession>
    <name>A0A8I6ZCG4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123412254</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044961137.1">
        <property type="nucleotide sequence ID" value="XM_045105202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZCG4"/>
    <dbReference type="SMR" id="A0A8I6ZCG4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0719410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0719410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0719410"/>
    </dbReference>
    <dbReference type="GeneID" id="123412254"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0596640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0596640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0596640"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0719410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0719410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0719410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412254"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF324">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="178"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="231"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="142"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0719410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="59073" checksum="2B0914ABDAC78C57" modified="2022-05-25" version="1">MTRTPRRQFSSHPLAYIFSPVVFPEGPPIHSVTVVTRALTHPRFTVASRSAQQLLPHLVATNNHRRSTAPLHSASARAECRRRSEEFGGMGMEYEPVPLAASSPKKPAAGGRAALKLLLALLLVGLAMRLLADRCASRLLPPTAPPGEDKALAAKAPPAQEEAGGGDGVPVTPSGAGSCDLFHGEWVHDSSGPAYTNATCRFIETPQNCMSNGRPDDGYLYWKWKPYGCEVPPFEGKTFLEDMRGKHWALVGDSILRNHVQSLLCLLSKVEDPTEVYHDKTYQSRRWHFPSYNFTLSLVWAPFLVKAEIFEDENGISSAEPRLTFDVLDANWVGQWSSFDYVIISTGQWFFKKAVYLEKGAVIGCHYCQDKSLREVSIDYSFRRALREAFRFITASAHRPVVFYRTWSPSHFEGGEWFSGGRCDRKAPFKPREAGDRALDNLMWRVERAEFAKAAAEDGAAGGEGRRLRLLDTFEMSLQRPDAHAGPYRAYQPFAKGAAAGKVQNDCLHWCLPGPIEAWNDIIMQMLAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1E0</accession>
    <name>A0A8I7B1E0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">UMP pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123415151</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044962638.1">
        <property type="nucleotide sequence ID" value="XM_045106703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1E0"/>
    <dbReference type="SMR" id="A0A8I7B1E0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073840"/>
    </dbReference>
    <dbReference type="GeneID" id="123415151"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0059720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0059720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0059720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123415151"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000003">
        <property type="entry name" value="Uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="UPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF4">
        <property type="entry name" value="URACIL PHOSPHORIBOSYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="87"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="294" mass="31058" checksum="2D121E79A1A96DA3" modified="2022-05-25" version="1">MPSPATATAAAAGAPLHRRPCAPRRPAQASPCSLAARAVVESARHPVPTPLLARRPRPSLAVARAASPDAATGARSPGSGGQMLVFVPPHPLIKHWVSVLRNEQTPCAVFRSAMAELGRLLIYEASRDWLPTITGEIQTPVAVSSVEFIDPREPVMVVPILRAGLALAENASSILPATKTYHLGLRRDEETLQPSVYLNNLPEKIPEGTRVLVIDPMLATGGTIVAAVELLVDRGVTSKQIKVVSAVAAPPALQKLSNKFPGLHVYAGMIDSEVDERGYIVPGLGDAGDRSFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2F9</accession>
    <name>A0A8I7B2F9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B2F9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0126660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0126660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0126660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0104510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0104510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0104510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0126660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0126660.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0126660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0126660.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="150" mass="17029" checksum="B303CF0C65F30743" modified="2022-05-25" version="1">MEFGGPILSPFQLKLSKLAKRRLCEPETDDSVNPKLSKSTARVGRLDPSELGWHSENSFEALSYHLPAPQPRVDLECALGTRMVSRQLGWEHHNIPHVRRPNGYIEWGAEVIDHHTFNLKGGEDHTHYICGAIYVSLCDYDVCPNFFNLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4I1</accession>
    <name>A0A8I7B4I1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123424386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044963921.1">
        <property type="nucleotide sequence ID" value="XM_045107986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4I1"/>
    <dbReference type="SMR" id="A0A8I7B4I1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099660"/>
    </dbReference>
    <dbReference type="GeneID" id="123424386"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0082100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0082100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0082100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0099660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0099660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0099660"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424386"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF893">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="43"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0099660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="521" mass="57489" checksum="F588DE50625312F3" modified="2022-05-25" version="1">MHFVIARTSREAHTSISSCISTSIQGDDMGAAPTNKMPHPHAVFVPFPAQGHITPMMKLAKIFHCKGFHVTFVNTEYNHRRLVRSRGPDAVVGLPDFHFATIPDGLPPSDADATQDIPSLCYSTMTNCLPHLKNLLRDLNGAAGVPQVTCVMADAVMSFCLDAAAELSVPCALFWTASACGFMGYHNFRFLLDEGLAPLKDEEQVKNGYLDTQVTQARGMSKHMRLRDFPTFFRTTDRGDVMFNFLMHEVEQSSRAAAVILNTFDELEQTALDAMCAILSLPVYTIGPLNFLAGQVVPDDGAQLAMIRPSLWREDHSCLEWLRGREPRSVVYVNFGSITTMSIQELVEFAWGLANCGYDFLWIVRNDLVKGDAAVVPPEFLEATEGRCLLASWCEQEAVLRHEAVGTFLTHCGWNSTMEGLSAGVPMLCWPFFAEQQTNARYACVEWGVGMEVGDDVRRGVVEARIREVMGGGGVGREMRRKAAEWSEMAVRVTTQPGGRSLANLESLLKDVLLVATRNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5L2</accession>
    <name>A0A8I7B5L2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IST1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123444145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976705.1">
        <property type="nucleotide sequence ID" value="XM_045120770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5L2"/>
    <dbReference type="SMR" id="A0A8I7B5L2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0281100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0281100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0281100"/>
    </dbReference>
    <dbReference type="GeneID" id="123444145"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0281100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0281100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0281100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444145"/>
    <dbReference type="OrthoDB" id="29853at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000003">
        <property type="entry name" value="IST1-like protein isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF81">
        <property type="entry name" value="OS01G0687700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0281100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35174" checksum="CD06589F715C2ACD" modified="2022-05-25" version="1">MSSLNSLFNRSSPFGTKCKTCLNLIISRIKLLRNRREMQLINMRKEMVQYLQTGQESIARIRVEHIIREQNILAAYEIVELFCEFVLARVPIVEAQKECPLELREAIASIIFASGRCSDLPELMHLRNLFTTKYGKEFVAAAMELRPDCGVNRTIIEKLSVKAPSAESKLKVLKAIAHEYNLDWDSSNTEAEFNKKYEDLLDDSGSSVRQGQTPKIESSPGSSISRDKPSILPVNDTSKNKTPESPKSPAVSSRAYAATKSNVAVQEHHPPAAERSSSAGPGSSDVLEKARAAIVAATRASAAAWTAAELVNKVKVTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B8P5</accession>
    <name>A0A8I7B8P5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LURP-one-related</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123395998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946959.1">
        <property type="nucleotide sequence ID" value="XM_045091024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B8P5"/>
    <dbReference type="SMR" id="A0A8I7B8P5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0463850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0463850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0463850"/>
    </dbReference>
    <dbReference type="GeneID" id="123395998"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0383940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0383940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0383940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0463850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0463850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0463850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395998"/>
    <dbReference type="OrthoDB" id="680369at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="137" mass="15107" checksum="B3FB103282A1D7FD" modified="2022-05-25" version="1">MDEVTQAVENLKKEWSQAVAQLEVCIAAIESCGKMGKGTEEAMSLPRLNGSAQDALQLLNALHCRLDLLAEQLPTFEEVQSGQATLGSWNEQYQRLRVSLRTANLQAKANIGKAAQEERELLLGGGEESTIRRRNLQ</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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        <source>
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    </feature>
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        <location>
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            <end position="206"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0572270.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
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</entry>
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    <reference evidence="2" key="1">
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BC08"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0359030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0359030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0359030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0359030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0359030.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0359030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0359030.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28966" checksum="FE02444BB3DAA9F7" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTSMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFYYVPASQSGEEGYLSGVSFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVIPLRGPLLPPVRGTRPHPCPYGSQGSQARVFPDPQVELPGLAGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BFJ4</accession>
    <name>A0A8I7BFJ4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BFJ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0518120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0518120.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0518120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0518120.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15993" checksum="BB20D853BC501D43" modified="2022-05-25" version="1">MNFQDVYTLQQALDVAPPPRGNSAQDRAEHTARQRRLLVAQEDERVMAEWRRRHPKDVAYEQEYWARRREEDTRRRREERLDGRRRKALASAQADLVNAGGSSFFTEEDGRWFDIWLSTSDDTYDDDDGADDWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGC8</accession>
    <name>A0A8I7BGC8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GS catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7BGC8"/>
    <dbReference type="SMR" id="A0A8I7BGC8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0456510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0456510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0456510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0549370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0549370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0549370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000046">
        <property type="entry name" value="Glutamate-ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000012">
        <property type="entry name" value="Glutamate-ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383:SF2">
        <property type="entry name" value="AMIDOHYDROLASE 2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383">
        <property type="entry name" value="NODULIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="3">
        <location>
            <begin position="519"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0549370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="853" mass="94722" checksum="D760B0A022BC4272" modified="2022-05-25" version="1">MDMEAKYADLRRAAEETAVVDAHAHDLLAAGSTLPFTRCFSEADGDALTLAPHSLPFKRSLRDIAALYDCDASFEKVEEFRRAQGLSSITSKCFQAANISALVVDDVSKFNEILELESHNAFAPKVYRIVGIETLAETIINEESLSGSSWTLDSFTEAFVAKLKSYPTQFRMLSVFTNKIVGLKSIAAHRSGLDIDPSVSKVDAEDGLQKDLATRRPLRITNKNLVDYIFIRSLEIAVEFHLPMQIHTGFGDRDVELRNCTPFHLRSLLEDKRFAKCQIVLLNASYPFSREASYLASVYSQVYLDFGFAFPKLSVQGITSSLKELLERAPIKKVMFSTDGYAFPETYYLGAKWARDIVYRVLLAACEDGDLTIQEAIEAVEDILRRNALHLYKLDVVHEKVINIDDNSTSSSSCVKKDDVILVRMVWNDASGQHRCRALPAERFYGIARNKGVGLGIAAVGFTSFRDAPAAGTNLTCAGEEIRLVADMSTLLRIPWSRNEEMVMADMITGSGEASEYCPRNALRKVIKVLLDEFNVTVMAGFENEFYLLRKSFSEGHEQWVPYDNSSYCSTSAFDGASSILKEAYSCLKAAEIVVEQMHAEGGNGQFEIALKYVLCTLAADNQIYAREIIKSVARKHGVIATFLPKPDLNELGSGCHVHLSLWENDQNVFMGSNKYSYHGMSNTGERFLAGVYHHLPSILAFTAPHPHSYDRIQPHTFTGAYLCWGKENREAPLRTSCPPGVPPDFVSNFEVRSFDGCANPHLGLAAIVTAGIDGLRRGLKLPPPTELNPADCASHERLPHDLLGSAEALAADEIFHELMGDKLVTSVIAMRKAEIEHYAKNPVAVHHLIHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BIV3</accession>
    <name>A0A8I7BIV3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIV3"/>
    <dbReference type="SMR" id="A0A8I7BIV3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0675800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0675800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0675800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0560770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0560770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0560770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0675800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0675800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0675800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0675800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="123" mass="14849" checksum="A0241671BEA48B9D" modified="2022-05-25" version="1">MTIMPTNVTWIFFIYEVCEPEIPTDPLVERLNLVEKENNYLKKMKKIEEKNMKLELHVADVVHDHKIKMDAMRLKMNAMRLKIRNIRKYVINKEVWYHYVVGLIIILVTILIIFVVVFKCFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJW1</accession>
    <name>A0A8I7BJW1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJW1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0734400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0734400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0734400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35691">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35691:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0734400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9564" checksum="06BD7972F92DACEA" modified="2022-05-25" version="1">MCTAIALGTWVFSVAGALLAIPVGIKRKSFAPLVFFGTTGTMLDIIMGISQCEREHAERQMKLLEAQNLATNASVEGESLTDSFTNVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJX6</accession>
    <name>A0A8I7BJX6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123412924</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044961819.1">
        <property type="nucleotide sequence ID" value="XM_045105884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJX6"/>
    <dbReference type="SMR" id="A0A8I7BJX6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0736780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0736780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0736780"/>
    </dbReference>
    <dbReference type="GeneID" id="123412924"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0611150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0611150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0611150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0736780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0736780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0736780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412924"/>
    <dbReference type="OrthoDB" id="551907at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31314:SF113">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR MPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31314">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0736780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="249" mass="27635" checksum="C4FB580954BFFFCC" modified="2022-05-25" version="1">MRGFERRGVRQYNRSEEPRMRWTEELHRQFIEAVDCLGGQDEATPKRILQLMGAKGVSISHIKSHLQMYRSSSSNTNSNGPANMSVDCRPDHRAVDVSRSSHGEDMAAASERIDASSYAVLPRGHRSSPSYQIPSIDEVFMSWEQSRGRLPWNSTMKSEKATGRARHADSRARQKNQRPTAGCDLTLSIGRCDEEAMVVISDADVSSTTTEEAAVVPARDRGAGDRRRSDTAGLNLDLNLDLAVSSSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJZ7</accession>
    <name>A0A8I7BJZ7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJZ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0739800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0739800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0739800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0613610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0613610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0613610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0739800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0739800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0739800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0739800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="65" mass="6994" checksum="724EB7009247FECB" modified="2022-05-25" version="1">MVAAGPRLPCLCMRSPGSGAAASLARHGRLAVVREVDPASRPRGRRDPVVAGVRRRLLQWPVRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-05-20" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>B2D0N5</accession>
    <name>B2D0N5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Respiratory burst oxidase homolog protein B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">NADPH oxidase RBOHB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC123449930</name>
        <name evidence="24" type="synonym">RBOHB1</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2008" name="Funct. Plant Biol." volume="35" first="347" last="359">
            <title>Identification and characterisation of barley (Hordeum vulgare) respiratory burst oxidase homologue family members.</title>
            <authorList>
                <person name="Lightfoot D.J."/>
                <person name="Boettcher A."/>
                <person name="Little A."/>
                <person name="Shirley N."/>
                <person name="Able A.J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="25" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="24" key="5">
        <citation type="journal article" date="2021" name="Front. Plant Sci." volume="12" first="0" last="608541">
            <title>The Barley (Hordeum vulgare ssp. vulgare) Respiratory Burst Oxidase Homolog (HvRBOH) Gene Family and Their Plausible Role on Malting Quality.</title>
            <authorList>
                <person name="Mahalingam R."/>
                <person name="Graham D."/>
                <person name="Walling J.G."/>
            </authorList>
            <dbReference type="PubMed" id="33679826"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="25" key="6">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Calcium-dependent NADPH oxidase that generates superoxide.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer and homodimer, stabilized by swapping the EF-hand motifs. Interacts with GTP-bound RAC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBOH (TC 5.B.1.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="EU566852">
        <property type="protein sequence ID" value="ACB56481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362538">
        <property type="protein sequence ID" value="BAJ93742.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MW520107">
        <property type="protein sequence ID" value="QVP25925.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983234.1">
        <property type="nucleotide sequence ID" value="XM_045127299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B2D0N5"/>
    <dbReference type="SMR" id="B2D0N5"/>
    <dbReference type="STRING" id="112509.B2D0N5"/>
    <dbReference type="PaxDb" id="4513-MLOC_5021.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414140"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414140.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414140.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414140"/>
    </dbReference>
    <dbReference type="GeneID" id="123449930"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0345110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0345110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0345110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0414140.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0414140.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0414140"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005646_6_0_1"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B2D0N5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000049">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000019">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000034">
        <property type="entry name" value="Respiratory burst oxidase homolog protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013623">
        <property type="entry name" value="NADPH_Ox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF141">
        <property type="entry name" value="MEMBRANE-BOUND NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08414">
        <property type="entry name" value="NADPH_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="170"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="527"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ACB56481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="BAJ93742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="QVP25925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0414140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="845" mass="94963" checksum="EB35DE6C9503997B" modified="2008-05-20" version="1">MADIEAGKPATESDTATLIPNSGSLEGSSRATKTTRFKDDDEVVEITLDVQRDAVSVQGVRPVAAEAAVAAARKRYDRSKSTAAVALKGLQFVTAKVGGDGWAAVEKRFNHLQVDGVLLRSRFGKCIGMDGSDEFAVQMFDSLARKRGIVKEVLTKAELKEFWEQLSDQGFDNRLQTFIDMVDKNADGRITSEEVKEIIALSASANKLSKIKERADEYTALIMEELDPDNLGYIELENLEALLLQPPSEAVASTITHSSKLSKALSMRLAPSKGMSPLHRYWQEFSFFVEENWKRIWVAALWLSICIALFVWKFIQYRNRAVFHIMGYCVATAKGAAETLKFNMALVLLPVCRNTITWIRSKTKIGAVVPFNDNINFHKVIAAGVAVGVVLHAGAHLTCDFPLLLHASDAKYEPMKPFFGEKRPPNYWWFVKGTAGWTGVVMVVLMSISFVLAQPWFRRNKLKPTNPLKKMTGFNAFWFTHHLFAIVYALLIVHGTSLYLTKEWYKKTTWMYIAYPVFLYLCERIVRLFRSNDAVKIQKVAVYPGNVLALYMTKPPGFRYRSGQYIFINCGAVSPYEWHPFSVTSAPGDNYLSVHIRTRGDWTSRLRTVFSEACRPPTEGESGLLRADLSVGITDSNARFPKLMIDGPYGAPAQDYREYDVLLLIGLGIGATPLISIVKDVLNHIQRGESVGGTEPDGSGKAKKKPFMTKRAYSYWVTREEGSFEWFRGVMNEVAEKDKDGVIELHNHCSSVYQEGDARSALIVMLQELNHAKKGVDILSGTSVKTHFARPNWRSVFKRIAVNHENQRVGVFYCGEPVLVAQLRQLSADFTHNTNTKFEFHKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F2CR82</accession>
    <name>F2CR82_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123407623</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK354134">
        <property type="protein sequence ID" value="BAJ85353.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044956725.1">
        <property type="nucleotide sequence ID" value="XM_045100790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CR82"/>
    <dbReference type="SMR" id="F2CR82"/>
    <dbReference type="PaxDb" id="4513-MLOC_58028.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742520"/>
    </dbReference>
    <dbReference type="GeneID" id="123407623"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0615730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0615730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0615730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0742520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0742520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0742520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407623"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_0_1"/>
    <dbReference type="OMA" id="YARKFPH"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2CR82">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
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        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF738">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ85353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0742520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="363" mass="38843" checksum="E580FFCFF5C15F13" modified="2011-05-31" version="1">MAEEGKGTGSNGGRGVRACVTGGAGFIGSWLVRKLLEAGYTVHATLRSIGDEGKAGLLRGLVPGGAPPERLVLFEADIYDAASFAPAIAGCHFVFLVANPSAHEAAASKYKTSAEAAADAVRVILRLCAESRTVKRVIHTASVTAASPLTKSSSAAAAVYGDFISESCWTLLDVNYPLRSVHFDKYIESKVLSEKELLSYNDGENPAFEVVTLPLGLVGGNTVLGYLPETMESVVAPVTKQEPCFMLPRILQRLLGSVPLVHVDDVCAALIFCMEQPALSGRFLCAAAYPTIHDILDHYGSKYPHLDLLRETDEVARVQPDKNKLGELGFEYKYGLKEILDESIDCAVRLGSLDASKLIVQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYM7</accession>
    <name>F2CYM7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123446672</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK356733">
        <property type="protein sequence ID" value="BAJ87948.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044979166.1">
        <property type="nucleotide sequence ID" value="XM_045123231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYM7"/>
    <dbReference type="SMR" id="F2CYM7"/>
    <dbReference type="STRING" id="112509.F2CYM7"/>
    <dbReference type="PaxDb" id="4513-MLOC_4713.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0390130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0390130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0390130"/>
    </dbReference>
    <dbReference type="GeneID" id="123446672"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0324550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0324550.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0324550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0390130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0390130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0390130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446672"/>
    <dbReference type="OMA" id="ANACSPQ"/>
    <dbReference type="OrthoDB" id="665906at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000006">
        <property type="entry name" value="ethylene-responsive transcription factor ERF014-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051032">
        <property type="entry name" value="AP2/ERF_TF_ERF_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985:SF87">
        <property type="entry name" value="AP2 DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="35"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ87948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0390130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="24135" checksum="81CFCAD3F6C4F4E7" modified="2011-05-31" version="1">MVKNHPGSGTSSRCVDAAAVPESGGRRPAAMAVRQYKGVRMRSWGSWVSEIRAPHQKRRIWLGSYATPEAAARAYDAALLCLKGSDAVLNFPSSSSASPPSPPHSVPADDLSPRSIQRAAAAAAAAFEATRIVVDDSCSSSAEATTPRTSVSVSTLGSADVQEHATSSMSRAASAGSPVGDHEELWTDLDAFASPKLMDLIAAGHATPFSSTWEEPEEDGEMMRLWSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYW5</accession>
    <name>F2CYW5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444929</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK356821">
        <property type="protein sequence ID" value="BAJ88036.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044977751.1">
        <property type="nucleotide sequence ID" value="XM_045121816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYW5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302680"/>
    </dbReference>
    <dbReference type="GeneID" id="123444929"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0252530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0252530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0252530"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0302680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0302680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0302680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444929"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31288">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31288:SF10">
        <property type="entry name" value="PROTEIN ESMERALDA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ88036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0302680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="553" mass="62249" checksum="1D605A4C5BF82E39" modified="2011-05-31" version="1">MQKQAYSRLGSFGGGAAPSPPPSPRRAWGRRPSAKGGAARRAARAVLAALLRRQAVFLFAPLLYVAAMLLYMGSVSLDGVPRIISRPAPGSLYRSPQLYARLRPEMDADNATDALATVWRHAYKGGVWQPCISNNTYGLPEPNGYIYVEANGGLNQQRTSICNAVAVAGFLNATLVIPNFHYHSIWRDPSKFNDIYDEDHFVQRLKNDVRVVDKVPGFIMERFSNNLSNVYNFKIKAWSPIQYYKDVVLPKLIEERVIRISPFANRLSVDAPPAVQRLRCLANFEALKFSKPITALSDTLISRMREKSVENNGKYVAVHLRFEEDMVAFSCCVFDGGDEEKKELDEARERGWRGKFTRPGRVIRPGAIRMNGKCPLTPLEVGLMLRGMGFSNKTSIYLASGRIYKAEKNMAPLLEMFPLLQTKETLASDEELAPFKNFSSRMAAIDYSVCVHSEVFVTTQGGNFPHFLLGHRRYLYGGHSKTIKPDKRRLAILFDSPRIGWKSLKRQLLSMRSHSDAKGIQIKRANESVYTFPSPDCMCHPNKSEHSKHIQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F2D7F9</accession>
    <name>F2D7F9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123435880</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Shoot</tissue>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The PPC domain mediates interactions between AHL proteins.</text>
    </comment>
    <dbReference type="EMBL" id="AK359821">
        <property type="protein sequence ID" value="BAJ91030.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK361244">
        <property type="protein sequence ID" value="BAJ92451.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366466">
        <property type="protein sequence ID" value="BAJ97669.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971532.1">
        <property type="nucleotide sequence ID" value="XM_045115597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D7F9"/>
    <dbReference type="SMR" id="F2D7F9"/>
    <dbReference type="PaxDb" id="4513-MLOC_54675.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0052700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0052700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0052700"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0052700.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0052700.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0052700"/>
    </dbReference>
    <dbReference type="GeneID" id="123435880"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0041260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0041260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0041260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0052700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0052700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0052700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0052700.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0052700.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0052700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123435880"/>
    <dbReference type="eggNOG" id="ENOG502R1P2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039808_7_1_1"/>
    <dbReference type="OrthoDB" id="1742671at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D7F9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.80:FF:000003">
        <property type="entry name" value="AT-hook motif nuclear-localized protein 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039605">
        <property type="entry name" value="AHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500:SF51">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="5">
        <location>
            <begin position="243"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="366"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ91030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAJ97669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0052700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="463" mass="48411" checksum="21105144CB9BFAED" modified="2011-05-31" version="1">MEAKDVAPLAAATAAAPTAPVPAPAPAPAPVSQAKPQPQPQPQPPPIASMPPPPHPFAHHQQQHHHHQQQQQQQQQPPAGSANPAAPMPGGGMRLSFDQMVGKPPGEHHPHQHQHQHPHQHQHQHQHQHQHQHPHQHHAPGPMLYAAPPPHAVAPPPGSNALGMGDLMRKKRGRPRKYAPDGSMALALAPLSSASGGAAPPPPPGQQHGFSISSPPSDPNAKRRGRPPGSGKKKQFEALGSWGISFTPHILSVKAGEDVASKIMSFSQQGPRTVCILSANGAISNVTLRQPATSGGLVTYEGRFEIISLSGSFLLAEDGDTRSRTGGLSVALAGSDGRVLGGCVAGQLTAATPVQVVVASFIAEGKKSKPAEPRKVEPMSAPPQMATYVPAPVASPPSEGTSSASSDDSGSPINHGGMPYNHSGGQQHQQQHMPPAYASGGWSLSAHHQNNNRHDADMKMMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F2DCV5</accession>
    <name>F2DCV5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123398493</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK361722">
        <property type="protein sequence ID" value="BAJ92926.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948892.1">
        <property type="nucleotide sequence ID" value="XM_045092957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DCV5"/>
    <dbReference type="STRING" id="112509.F2DCV5"/>
    <dbReference type="PaxDb" id="4513-MLOC_38595.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513340"/>
    </dbReference>
    <dbReference type="GeneID" id="123398493"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0426520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0426520.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0426520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0513340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0513340.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0513340"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398493"/>
    <dbReference type="OMA" id="ELKWALP"/>
    <dbReference type="OrthoDB" id="694638at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016972">
        <property type="entry name" value="UCP031279"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33526:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33526">
        <property type="entry name" value="OS07G0123800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031279">
        <property type="entry name" value="UCP031279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAJ92926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513340.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="154" mass="16290" checksum="46FCFBABFF4016BB" modified="2011-05-31" version="1">MSPGSGKLRWLWRAPARAFGRARDFYVRSITGCARYVPADAAFGAYPVLVPAPLPRSQSCGSGAGWDGGEEDLRELIRAASQRRDGEQQRQAAHLHAVPRSQSVAGAASMARIDEDAPCEFASGAALYSRSQSYAGGALGARKSLCHSKVAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDB2</accession>
    <name>F2DDB2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123444097</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK361879">
        <property type="protein sequence ID" value="BAJ93083.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976648.1">
        <property type="nucleotide sequence ID" value="XM_045120713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDB2"/>
    <dbReference type="SMR" id="F2DDB2"/>
    <dbReference type="FunCoup" id="F2DDB2">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279760.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279760"/>
    </dbReference>
    <dbReference type="GeneID" id="123444097"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0231830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0231830.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0231830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279760.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444097"/>
    <dbReference type="OrthoDB" id="655701at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF243">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="9"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="269"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="430"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="527"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="536"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ93083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279760.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="578" mass="62256" checksum="A6694A4529E6AE77" modified="2011-05-31" version="1">MAPIRRAAQRPDFASHPSDLELITTYLTPWSRNRERPWKFIHEADVYAARPQDLARAYAPATASDGQESWYFFTILRTKSRRGQRKSRTVGSGDHGCWHSERAAKPLFAGIGHSRQIGYRQAFSFATKEDGRLVRSGWLMAEIGLNPDASSPDEVVLCKVYRSPRVKTDQRSTAAAAAAAATGVGPQRIARGKASEDSTSSEEGTPRAGPGRYSAQPARASASTSGTLSMVESDSDSEQDSTSLGGTSPSATPPRIPRIPTVPSDESAQRPDFSAHPSDQVLIKSYLTPRVASGEHPCHFTHDADVYTASPGALTRTYSPAMASDGEKAWYFFTVLPAKSAHGQRRPRTVGTGEGCWHSEAGVKPVLDGDHPIGWRQFFSFMSKEEGQRVRSIRTGWIMVEIGLDHGQQEGPSDEPVLCKVYRSPRAGPVVEPKAAAGRKRNSKRRLDTSGAGTPVLTLGHALGTRNSTATATATATATSSSGRKKRKTDSRNPGPVLRPARGVLKLTRPATPGRKKKDLCVRCGVEPAESHSGTAEEEDGSETDLVEDDSMTGESAAIHGNEAGESSGGARTFYQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F2DG41</accession>
    <name>F2DG41_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AK362858">
        <property type="protein sequence ID" value="BAJ94062.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DG41"/>
    <dbReference type="SMR" id="F2DG41"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0402410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0402410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0402410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0485940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0485940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485940"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048531">
        <property type="term" value="F:beta-1,3-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000002">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025298">
        <property type="entry name" value="DUF4094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF307">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13334">
        <property type="entry name" value="DUF4094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF4094" evidence="14">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAJ94062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="396" mass="44271" checksum="8FE95DFF30852D71" modified="2011-05-31" version="1">MKAKSGGAAAGERRPVLSRTILLLCACSFGLGMLFTDRFGAMPDLKSPVVAQRRRQEEELQVVSEDFVAKTKPSDDRDVMGEVAKTHEAIQYLDKSIATLQMELAARRSKHELLESADGVMQDRKKAFVVIGINTAFSSKKRRDSVRETWMPQGEKLKKLEEEKGVVIRFMIGHSPASNSALDQAIDVEDAIHHDFLRLDHVEGYHKLSAKTKTFFSTAVASWDADFYVKVDDDVHVNLGMLLTTLGRQKLKPRVYIGCMKSGPVLSDKSSKYHEPEFWKFGEDGNKYFRHATGQIYAISKDLATYISVNKPLLHKFANEDVSLGAWFIGLDVEHIDDRDMCCGTPPDCEWKAQAGNACVASFDWRCSGVCNPVERLKDVHARCGEGDDAIWSASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F2DK78</accession>
    <name>F2DK78_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Peroxidase</fullName>
            <ecNumber evidence="4 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC123411638</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 19"/>
    <dbReference type="EMBL" id="AK364296">
        <property type="protein sequence ID" value="BAJ95499.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960536.1">
        <property type="nucleotide sequence ID" value="XM_045104601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DK78"/>
    <dbReference type="SMR" id="F2DK78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713280.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713280.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713280"/>
    </dbReference>
    <dbReference type="GeneID" id="123411638"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0713280.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0713280.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0713280"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411638"/>
    <dbReference type="OMA" id="HCDSFLF"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DK78">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="7 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019615" description="Peroxidase" evidence="19">
        <location>
            <begin position="29"/>
            <end position="357"/>
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            <position position="71"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <ligand>
            <name>heme b</name>
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            <name>Fe</name>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50873"/>
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        <source>
            <dbReference type="EMBL" id="BAJ95499.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0713280.2"/>
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    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    <sequence length="357" mass="37592" checksum="E0F4128B87BB4D12" modified="2011-05-31" version="1">MASSGSLAGLAVLLTAVLCLQLPVHSRAQLRVGFYNTTCPNAEAIVRQAVTAAFATNSGVAAGLIRLHFHDCFVEGCDSSVLLSVNPGGGTTERDAAPNNPSLRGFEVVNAARAALEQSCPRTVSCADILAFAARDSVNITGSNAFYQVPSGRRDGNLSTDAGAFTLPGPNLTAAGLVTGFEMRGLNAEDMVVLSGSHTLGRSHCSSFIFKNRERLASGTISPAYQALLEALCPANTGQFTNVTTEIDLSTPVVLDNNYYKLVQLNLGLHFSDDQLIRNATLKAFVDAFAANETLWKDKFIAAMIKMGNIAPKTGTQGQIRLNCSLVNPSSSSSSASYAGAIEMLRRPGSHDKVAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DK95</accession>
    <name>F2DK95_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123399327</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="AK364313">
        <property type="protein sequence ID" value="BAJ95516.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044949675.1">
        <property type="nucleotide sequence ID" value="XM_045093740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DK95"/>
    <dbReference type="SMR" id="F2DK95"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0443800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0443800"/>
    </dbReference>
    <dbReference type="GeneID" id="123399327"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0367550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0367550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0367550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0443800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0443800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0443800"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF40">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="18"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="325"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ95516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0443800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="581" mass="63149" checksum="00C80DAD8781E0E2" modified="2011-05-31" version="1">MAAAAAAAEAEEGRAATKYVLITGGVVSGLGKGVTASSVGVVLKSCGLRVTCIKIDPYLNTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDVTLTRENNITTGKIYQSVIEKERKGAYLGKTVQVVPHVTDEIKQWIQSVSSVPVDGQTCPADVCVIELGGTVGDIESMPFIEALRQLSFSLGKDNFCLIHVSLVPVLGVVGEQKTKPTQHSVRELRALGLTPDLLACRSAQPLIGSAKEKLSQFCHVPVENILNIHDVPNIWHVPLILRNQKAHEAIIKQLNLSRSAGPPELRDWTLMAESYDNLSTSVKIALVGKYTNLSDSYLSVVKALLHASVACSQKPSIQWVSASDLEDAAAASAPDAHAKAWETLKGSSCILIPGGFGDRGISGMILAAKYARENKVPYLGICLGMQISVIEMSRHVLGLGNADSEEFKTDTPDRVVMYMPEVSKTHMGNTMRLGCRRTFFRKPDCLTSKLYGNPPHVDERHRHRYEVNPSFVPMLENAGLQFVGCDESGNRMEIVELQDHPFYIGVQFHPEFKSRPRKSSPPFTGLILAATERMRTLTNVPNGGAGASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DN90</accession>
    <name>F2DN90_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123396695</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK365358">
        <property type="protein sequence ID" value="BAJ96561.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044947488.1">
        <property type="nucleotide sequence ID" value="XM_045091553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DN90"/>
    <dbReference type="SMR" id="F2DN90"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0475390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0475390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0475390"/>
    </dbReference>
    <dbReference type="GeneID" id="123396695"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0393780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0393780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0393780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0475390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0475390.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0475390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396695"/>
    <dbReference type="HOGENOM" id="CLU_101473_3_0_1"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAJ96561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0475390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="106" mass="11800" checksum="16054E3878B82584" modified="2011-05-31" version="1">MVFGRSKPSSSTPSPEASTSSASAAACSELRAAYHECFNRWYADKFAKGQWNKDDCAADWHKYRACLEEHLEDKHLRQILLESETSPSYAQLDPNSSSRQGSAPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0L8</accession>
    <name>F2E0L8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123430868</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK369689">
        <property type="protein sequence ID" value="BAK00890.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044970637.1">
        <property type="nucleotide sequence ID" value="XM_045114702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0L8"/>
    <dbReference type="SMR" id="F2E0L8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197540"/>
    </dbReference>
    <dbReference type="GeneID" id="123430868"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0164020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0164020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0164020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0197540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197540"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430868"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000310">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1103">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK00890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="358" mass="38630" checksum="6993CFDF3CED7B17" modified="2011-05-31" version="1">MGKGRAPCCAKVGLNRGSWTPEEDMRLIAYIHKYGHANWRALPKQAGLLRCGKSCRLRWINYLRPDLKRGNFTVEEEDTLIKLHNMLGNKWSKIAACLPGRTDNEIKNVWNTHLKKRVAASAGEHKKPGPKTKKKTTCAAPSPSPSPSSSTTTATTTTTNCSSGESGEQSNTSSKEPEPELDKIEIPMLDLGFDIDIDMLLDAVPETHCPAVSSAATSSCTSASPPCVADDDDALLDLPEIDIVPELWSIMDGACVETAQAPWGNAAPCQGNGAEATADEDDGKEWWLEDLEKELGLWGPIEDYQHQQQPDPLGRVGPLSASVDDPVSCYFQAGPATSAVHVQAPEASAVLTDIPMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0R9</accession>
    <name>F2E0R9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401443</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK369740">
        <property type="protein sequence ID" value="BAK00941.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044951193.1">
        <property type="nucleotide sequence ID" value="XM_045095258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0R9"/>
    <dbReference type="SMR" id="F2E0R9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556980"/>
    </dbReference>
    <dbReference type="GeneID" id="123401443"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0462650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0462650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0462650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0556980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0556980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0556980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401443"/>
    <dbReference type="OrthoDB" id="21643at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12226">
        <property type="entry name" value="RRM_NOL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000685">
        <property type="entry name" value="RNA-binding (RRM/RBD/RNP motifs) family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034138">
        <property type="entry name" value="NOP8_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099:SF0">
        <property type="entry name" value="GERM CELL NUCLEAR ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="222"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="401"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="456"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="509"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="575"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="318"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK00941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0556980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="599" mass="66041" checksum="50BF4A01642CAFAF" modified="2011-05-31" version="1">MAATAAAEADGGDAVITRLFVGGLAEGVSAADLEAVFGSIGRVAGVEFVRTGGRSFAYVDFQCPSHKALAKLFSTYNGCKWKGGKLKLEKAKEHYLVRLKREWEQEAAAAAAQEMPAKDDVDKQEEKEKRRLDKDALESSKVNIYFPRLRKLKPLPFKGSGKHKYSFRNIEVPSYPIHFCDCEEHCGPPEKANEEYAAVLKRVAFQKERNIMNSVMSKLFEKDTEQIDSSEVQKGDAETDPSDAEAEPSDTETEPSDSDNDLRMEETTESHEEDLDDLQMEETDDPSEEELDDDLVINIAPRKANNSAVQLNRANQSVKKDERFKKRQHFDEISPQKKRQKSEDLSEPRNQKQSTSVIADRRTTGKSLPYVSVAIQNEQKSSALSGKGMHGFSSVLDRAKSSAGLQGVDTLTGRSTKSEGSQNELPTEPKKGSVWTQKSSWRDLVGGMGATSFSISQVLPNTNPAPPKLEPATETSVACTESRREVTSLGMTSLESPEAITQPLAKQNLLSSTGTPSAGTTVGSAGHEIRGHDENNKVEEPRVVPRITISEVCPFMRNEESIQQWSKAKKVLTGFIKRGDEKKAGSSNSRRGKPPSRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F2E3J9</accession>
    <name>F2E3J9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123427464</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK370722">
        <property type="protein sequence ID" value="BAK01921.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967455.1">
        <property type="nucleotide sequence ID" value="XM_045111520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E3J9"/>
    <dbReference type="SMR" id="F2E3J9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0187130.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0187130.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0187130"/>
    </dbReference>
    <dbReference type="GeneID" id="123427464"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0187130.3">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0187130.3"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0187130"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063973_0_1_1"/>
    <dbReference type="OMA" id="SRASWVY"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E3J9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000226">
        <property type="entry name" value="E3 ubiquitin ligase BIG BROTHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033276">
        <property type="entry name" value="BB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400:SF11">
        <property type="entry name" value="OS04G0571200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK01921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0187130.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="253" mass="27954" checksum="B98A62F6F34AA96E" modified="2011-05-31" version="1">MSMEVLHDTTGKKEAAVYYMDAPLPYAIEENFGGCFFEDDVDLAQVLQDQEIVYQLIQGNDGSGSSRSHSDPSSSCGHPRKSGGRSLTRGANYESQLAVDEALARDLQAMEDQIAKAAIEENNRKEGRKPAGFSTSNNGNTSVSRSSQVLTEDGVDPDNMTYEELQQLGETIGTHSKGLPEDVIALLKSSTYKIGIFSRKEKHDECVICCMAYKNRDKLTTLPCQHQYHRTCVAKWLKINKVCPVCNKEVFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2E6S3</accession>
    <name>F2E6S3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123412824</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK371847">
        <property type="protein sequence ID" value="BAK03045.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044961705.1">
        <property type="nucleotide sequence ID" value="XM_045105770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E6S3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703260"/>
    </dbReference>
    <dbReference type="GeneID" id="123412824"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0583450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0583450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0583450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0703260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412824"/>
    <dbReference type="OrthoDB" id="1897957at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021924">
        <property type="entry name" value="DUF3537"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31963">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31963:SF2">
        <property type="entry name" value="ZINC FINGER CONSTANS-LIKE PROTEIN (DUF3537)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12056">
        <property type="entry name" value="DUF3537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK03045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="474" mass="52596" checksum="4126278D7D6C1473" modified="2011-05-31" version="1">MANGDRLAGAPSPPPMPPPLPPSLSRPSNRAPSENHLAISVPLLSLADADAPLARWLRRLEAFLAVSGLSASTPLGVAFAASALAVVGVALPAVAVSVSPCRKRERVCEEFEVEMFEVCVMMSQAAAAAVAVACVSRKMAMYGLRKFLFVDPELGMRIRFQKEYVVRIQDFFRTLMWWILPCLAVKITREMFRFSHMFHESIWRSCIVLFASIMSWMYLTTIVLSSCMLFNLVCNLQVIHFDDYGKVLEQDADPLVYLKEHLQLRHNLSKISHRFRMFLLLLFFSVTASQFAILYKTTAYNGPINFTNGGDIAVSSVVQVVGLVLCLHGAAKISHRAQNIAAIASRWHALATCSSDSTYVSTPNDSGNLVPFPAHMFLRDCSESDLECMESGSMQGNSHNTAQLASYMSSYHKRESLVLYLLTNPGGITLYGWIVDRTFLNTILMLELTLVLFVLSKTIVIPAKTLVNSYMSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F2E8T3</accession>
    <name>F2E8T3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123397670</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Flower</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="AK372557">
        <property type="protein sequence ID" value="BAK03755.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044948140.1">
        <property type="nucleotide sequence ID" value="XM_045092205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E8T3"/>
    <dbReference type="SMR" id="F2E8T3"/>
    <dbReference type="PaxDb" id="4513-MLOC_53147.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0501140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0501140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0501140"/>
    </dbReference>
    <dbReference type="GeneID" id="123397670"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0415960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0415960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0415960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0501140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0501140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0501140"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397670"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WAPRIAD"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E8T3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000088">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF71">
        <property type="entry name" value="OS03G0757000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK03755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0501140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="491" mass="53646" checksum="7F595AFC62048C32" modified="2011-05-31" version="1">MAEAGDATTATARSSSPLHIVVFPWLAFGHIIPYMQLSEQLARRGHAVTFVSTPRNLARLRPVPEDLRPRIRLLPLPLPRVDGLPDGAESTADVPPEKGDLLKVVFDGLAAPFADFLAGACSGGGVGEGAIGLCKKPDWIFVDFAHHWLPPIAEQHKVPCALFSIFPASFIAFAGTKAANEARPRVTAEDLTAQPPWIPFPTPIAHRLYEAEQMVYVFRPKASGLCDAFRFWETERQCTLFILRSCREVDGALCPLIADLFGKPLALSGLLAPYDAARAAQEAGRENDDEKSASLMRWLDEQPARSVLYVAFGSEAPLTAENIRELAAGLELSGARFLWALREASAPLLPDDGFAERVAGRGVVRAGWVPQVRVLAHGAVGGFLTHAGWSSLMESFLFGHPLVMLPLFADQGLTARVMAARAVGLEVARDERDGSFGRADLASTVRRVMAREDEEGKALARNAREFQEMLCDRAKQEEYVDELVEHLRRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2ED70</accession>
    <name>F2ED70_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA biogenesis protein RRP36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123400008</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with 90S and pre-40S pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RRP36 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK374095">
        <property type="protein sequence ID" value="BAK05292.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950337.1">
        <property type="nucleotide sequence ID" value="XM_045094402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ED70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497790"/>
    </dbReference>
    <dbReference type="GeneID" id="123400008"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0412960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0412960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0412960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0497790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0497790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0497790"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123400008"/>
    <dbReference type="OrthoDB" id="448446at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009292">
        <property type="entry name" value="RRP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06102">
        <property type="entry name" value="RRP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK05292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0497790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="279" mass="31763" checksum="4DE66C1450C8C73C" modified="2011-05-31" version="1">MKGRSSHRGAAPTSAAASTSSKRDPEDEPVSASEESGDEEQDVSSSSGSESESENDELTDRERKLERVLADVPFGELQRARADGSLGARGVSAAAAAQKKARRESRKRPMEISTNVRPPRLREVIQVPKKVVRDPRFEPVYGAVDKEGFRKRYNFLFDHDLPAEKEKLQKSIKKLKDPNAIEEVKNQITWIDKQLRSNPQKNVESEILRGHIKKEREAAKAGKRPYYLKKSEIRERKLMDKYNELKEAGKLDSFMEKRRKKNASKDHRFMPYRRDGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F2EKV7</accession>
    <name>F2EKV7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Potassium channel</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Seed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Potassium channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the potassium channel family. Plant (TC 1.A.1.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AK376785">
        <property type="protein sequence ID" value="BAK07979.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EKV7"/>
    <dbReference type="SMR" id="F2EKV7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0669120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0669120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000074">
        <property type="entry name" value="Potassium channel KAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000139">
        <property type="entry name" value="Potassium channel SKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045319">
        <property type="entry name" value="KAT/AKT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743:SF14">
        <property type="entry name" value="POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11 15" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 16" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4 16" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="8 16" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="401"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="577"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="610"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="674"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAK07979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="797" mass="90440" checksum="D5ABACFEAB7B976A" modified="2011-05-31" version="1">MARGARSRRAPAGEEEAEKEYEVDVEPDRWKSSSSSRSSRLELMGRLPPLRRHSRIPGADGDGEGGLLRGLVIHPDNKLYRLWTRFIVGWAVYSSFVTPFEFGFFRGLPRKLFFLDIVGQVAFLIDIVLKFLVAYRDPDTYRIVYNPTSIALRYCKSSFIFDLLGCFPWDAIYKASGRKEELRYLLWIRLTRVLKVREFFTDLEKDIRVNYLFTRIVKLILVELYCTHTAACIFYYLATTLPESVEGYTWIGSLQLGDYSYSHFREIDLATRYVTSMYFAIVTMATVGYGDIHAVNVREMIFVMIYVSFDMVLGAYLIGNMTAMIVKGSATERFRDKMKEVIRYMNRNRLGKDIREQIKGHLRLQYESSCTEASVLRDIPVSIRAKISQTLYMPYIERTPLFKGCSEEFLQQIVIRLQEEFFLPEEVILEQGSAVDQLYFVCQGALEGVGIGEDGQETILMLEQGNSFGEIAILCNIPQPYTVRVCELCRLLRLDKESFAHILEIYFADGRKLLSNLTESNEYGKRVKHVESDITFHIGKQEEELTLRVNNAAFYGDLHQLTGLIRAGANPKNSDYDGRSPLHLAACRGYEDVVRFLIHEGADINLTDKFGNTPLLEAVRQGHGRVASILFSKGAKLNLENAGSHLCIAVSKGDSDFIQGALAYGADPNSKDYDHRNPLHIAAAEGLYIMAKLLVDAGASVFAMDRRGTTPLDEGHKSGSKPLIMLLEQAKADELSKFPTRGEEVRGKFRSLNLCIITCTLFICDNKNFCWQIRCIQDDAPCSLTIHGILMPSEKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2EL52</accession>
    <name>F2EL52_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123447793</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK376880">
        <property type="protein sequence ID" value="BAK08074.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044980402.1">
        <property type="nucleotide sequence ID" value="XM_045124467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EL52"/>
    <dbReference type="SMR" id="F2EL52"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0342920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0342920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0342920"/>
    </dbReference>
    <dbReference type="GeneID" id="123447793"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0286140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0286140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0286140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0342920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0342920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0342920"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447793"/>
    <dbReference type="OMA" id="FKVMLKM"/>
    <dbReference type="OrthoDB" id="1078367at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090297">
        <property type="term" value="P:positive regulation of mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF18">
        <property type="entry name" value="PROTEIN OSB1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK08074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0342920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="294" mass="33573" checksum="73745BBB847BAA48" modified="2011-05-31" version="1">MLRSLAAAARSPAAAAWRRLLHHGRGIGGGEEAESMAYRMSMLRPPTVARKKDIISSNSCSLIGRLNAPVRLHLNSSEEDPKAYTFLCVTPSSSSSSSSTSSNFQVTLQMKGDLANVCLKHLKYKDLVHVSGFLNSYHKVSETGELYVYYKIHVKELNYVYDPKKARNDNDSVDPASTPSADTQTLEETKCRERLRLWQVFFASPYEWWDNRQYKPYAGCPDFKHKDTREQLWLHPDDPPWVIKQLKLIDQQTAESGRRDGKGRLTNPKWDAQDFNYSDECHDDEQDIRRQANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>M0X3W4</accession>
    <name>M0X3W4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123441089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044973550.1">
        <property type="nucleotide sequence ID" value="XM_045117615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0X3W4"/>
    <dbReference type="SMR" id="M0X3W4"/>
    <dbReference type="PaxDb" id="4513-MLOC_5681.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061870"/>
    </dbReference>
    <dbReference type="GeneID" id="123441089"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0049160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0049160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0049160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0061870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0061870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0061870"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123441089"/>
    <dbReference type="eggNOG" id="KOG0691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="NPLHFQW"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0X3W4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45098">
        <property type="entry name" value="DNAJ DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45098:SF1">
        <property type="entry name" value="DNAJ DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0061870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="303" mass="33015" checksum="72ACA63DFF276BC4" modified="2013-04-03" version="1">MAAMGQEGDDVDHYEVLRLPSGEEGAALSVEQIEKAYRTQSRLRHPDKRPDDPNATADFQSLASSYKFLRDESLRRQFDARLRGRREAAARAAATGVKRRKAVSDLEERERAFAAGGGPAVDPAELARREDKRKAADVKRELDEFFAAKKSGVSGSASTPPHGDKKGGTPENGPKTDKGKILKVSWEGGADYYTAAKLDEIFKQFGMVEDIVIKTSKSKRKGSAIVVMASKEAAQTALKNHSVYNVFPVPLFVASVQESGGLPARSTQTPEPRTSNIDGTGFSDLEASVFQKLQEAQKRKKCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>M0XN17</accession>
    <name>M0XN17_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123401792</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951576.1">
        <property type="nucleotide sequence ID" value="XM_045095641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XN17"/>
    <dbReference type="SMR" id="M0XN17"/>
    <dbReference type="FunCoup" id="M0XN17">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XN17"/>
    <dbReference type="PaxDb" id="4513-MLOC_61512.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607370"/>
    </dbReference>
    <dbReference type="GeneID" id="123401792"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0503500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0503500.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0503500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0607370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0607370.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0607370"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1285">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XN17"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XN17">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF46">
        <property type="entry name" value="CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="36"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0607370.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="643" mass="69175" checksum="E16FF40FE61C9C43" modified="2013-04-03" version="1">MEGALVTSNFSMADLYPAKANAAHAPRSRRLSYISPAASSAAAPNPSYRRKKSTSPPPPSAAATATVLTSPPKPVEQQETEEHLVDRSVNNATGTRVATARASRASAAPRQAPRSSRPRRRPSTSLQAAFCNALEEAINTFVDPPVLRPSVDPRHVLSANFAPVDELPPTPCPVVRGVIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPTTPGSDPVLCSRYVQTYKYLVERDAGEPVLPNVFSGFHGLAGMARGAVTAARVLTGQMNPMEGVGLANTSLAVFGGRLYALGESDLPYAVRVDPATGEVTTLGRCDFGGRLSMGMTAHPKKDPITGELFAFRYGPMPPFVTYFRFDPAGNKGADVPIFSVKQPSFLHDFAVTEHYAIFPEIQIVMNPMGMVVGGGSPVGADPGMVPRLGVIPKYAADESEMRWFEVPGFNMMHSVNAWEEAGGQEIVMVAPNILSIEHTLERMELIHASVELVRINLRTGNVTRTPLAAANLDFGVINPACLGRRNRYGYFGVGDPMPKIGGVAKLDFDRAGHGDCTVARRDFGPGCFAGEPFFVSDDVEGNGDEDDGYVVCYVHDEGTGDNRFVVMDAQSPDLDIVAEVQLPSRVPYGFHGLFVRQAELRSQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>M0XYL7</accession>
    <name>M0XYL7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box/kelch-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123427808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967872.1">
        <property type="nucleotide sequence ID" value="XM_045111937.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967873.1">
        <property type="nucleotide sequence ID" value="XM_045111938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XYL7"/>
    <dbReference type="SMR" id="M0XYL7"/>
    <dbReference type="FunCoup" id="M0XYL7">
        <property type="interactions" value="1325"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XYL7"/>
    <dbReference type="PaxDb" id="4513-MLOC_64217.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195300"/>
    </dbReference>
    <dbReference type="GeneID" id="123427808"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0162070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0162070.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0162070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0195300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0195300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0195300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427808"/>
    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XYL7"/>
    <dbReference type="OMA" id="VRSGENH"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0XYL7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000007">
        <property type="entry name" value="F-box/kelch-repeat protein SKIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052439">
        <property type="entry name" value="F-box/Kelch-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122">
        <property type="entry name" value="GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122:SF6">
        <property type="entry name" value="OS04G0619300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0195300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="49481" checksum="5787382216317CFA" modified="2013-04-03" version="1">MEDLQDCNSKSLVAVPGSLVLHVFRLLGQQDNSWQKYALAYFLLVRNEYFPREPRKHSAVNGQLVHCCVSSDLGSKEVEVEEQNAVVKTQSGGDSSSNGSNDCFLPGLHDDLAQDSLAWTSRSDYPSLSCLNKKFSTLVNGGYLYKLRRKYGIVEHWVYLACSLMPWEAFDPSRNRWMRLPRMPCDDCFSCADKESLAVGTQLLVFGREYAGLAIWMYNLLTRHWSRCTPMNLPRCLFASGSCGEIAIVAGGCNGTGQVLRSAELYNSEAGQWETLPDMNLPRRLSSGFFMDGKFYVIGGVTSEGHSLTCGEEYDLDTRTWRRIHDMYPGGTSASQSPPLIAVVNNQLYAADQSTNVVKKYDKASNTWNIVKPLPVRADSSNGWGLAFKGCGDRLLVIGGHRGPRGEVILLHSWCPEDGNGVADWEVLSVKERAGVFVYNCAIMGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>M0ZDF5</accession>
    <name>M0ZDF5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123426842</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966677.1">
        <property type="nucleotide sequence ID" value="XM_045110742.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966687.1">
        <property type="nucleotide sequence ID" value="XM_045110752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0ZDF5"/>
    <dbReference type="SMR" id="M0ZDF5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027380"/>
    </dbReference>
    <dbReference type="GeneID" id="123426842"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0021590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0021590.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0021590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027380.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426842"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0ZDF5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF21">
        <property type="entry name" value="OS02G0827900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027380.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="562" mass="62909" checksum="3E86845181BDB857" modified="2013-04-03" version="1">MASSASVAALVSLPFPTCPSSDDSDDAKPLPPPPTSGQTFPPPQQHQQRPQWWQLERDCNVAMKALARAGEVDQVLALFTELRASRAGSDGASPPNVLCYNTLVNALAEAGRVEEAHHAFDEMLAAGVAPNASSLNILVKLHSWRSARFDLAYKVIIRLQELGVEADVGTYSTLVTGLCRVGRVEEAWGVLEWMLEVGCRPMVQTYTPIVHGYCHEGRVDEAKELMSTMVNAGCPPNVVTYNVLIRALCDAARFDEVRQVLTDSRTKDWKPTTVTYNTFMNGLCKKGMAKEALEQLDVMLGEGLDPTDFTFSILLNCLCHDSRIPDAICLLERSTSLKCYAGVVAYNTVMSRLCEMGHWMGVLKLLTDMIKRGVMPSTRTFNIVIRSLCFGRKFSIAKSLASNQGFAANVVTYNTLIYFVFYYHRKLSEVKHLIFDMLAERIAPDEVTYTIIVDGFCRDGFFDTATGYFLESLKIGLSRDLFAVLTNRLAHNGKIWETIHIFKGMEEKGFLPDNSIFDLTIRTFCRAGYCNEREMFKLNFILDTMWESSEVNPFVYRKEGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-07-05" modified="2026-01-28" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>Q6RYF4</accession>
    <name>Q6RYF4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123449996</name>
        <name evidence="20" type="ORF">615K1.1</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Mol. Genet. Genomics" volume="268" first="488" last="499">
            <title>Cereal genes similar to Snf2 define a new subfamily that includes human and mouse genes.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Echenique V."/>
                <person name="Busso C."/>
                <person name="SanMiguel P."/>
                <person name="Ramakrishna W."/>
                <person name="Bennetzen J.L."/>
                <person name="Harrington S."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="12471446"/>
            <dbReference type="DOI" id="10.1007/s00438-002-0765-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2004" name="Science" volume="303" first="1640" last="1644">
            <title>The wheat VRN2 gene is a flowering repressor down-regulated by vernalization.</title>
            <authorList>
                <person name="Yan L."/>
                <person name="Loukoianov A."/>
                <person name="Blechl A."/>
                <person name="Tranquilli G."/>
                <person name="Ramakrishna W."/>
                <person name="SanMiguel P."/>
                <person name="Bennetzen J.L."/>
                <person name="Echenique V."/>
                <person name="Dubcovsky J."/>
            </authorList>
            <dbReference type="PubMed" id="15016992"/>
            <dbReference type="DOI" id="10.1126/science.1094305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="5">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY485643">
        <property type="protein sequence ID" value="AAS58474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983325.1">
        <property type="nucleotide sequence ID" value="XM_045127390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6RYF4"/>
    <dbReference type="SMR" id="Q6RYF4"/>
    <dbReference type="STRING" id="112509.Q6RYF4"/>
    <dbReference type="PaxDb" id="4513-MLOC_29110.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416190"/>
    </dbReference>
    <dbReference type="GeneID" id="123449996"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0346920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0346920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0346920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0416190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0416190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0416190"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007565_1_0_1"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q6RYF4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000010">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF46">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="17">
        <location>
            <begin position="340"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="19">
        <location>
            <begin position="612"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="18">
        <location>
            <begin position="815"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="824"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="859"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="830"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="866"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AAS58474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0416190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1218" mass="135811" checksum="25C614E9FEC11B44" modified="2004-07-05" version="1">MLTKFETKSNRVKGLAFHPRRPWILASLHSGVVQMWDYRMGTLLDRFDEHDGPVRGVHFHKTQPLFVSGGDDYKIKVWNYKTHRCLFTLHGHLDYIRTVQFHDEHPWIVSASDDQTIRVWNWQSRTCVAVLTGHNHYVMCASFHPKEDLVVSASLDQTVRVWDIGALRKKSSSPADDIMRLTQMNTDLFGGIDAVVKYVLEGHDRGVNWASFHPTLPLIVSGADDRQVKLWRMNDTKAWEVDTLRGHMNNVSCVLFHAKQDIIVSNSEDKSIRVWDATKRTGIQTFRREHDRFWVLAAHPEMNLLAAGHDSGMIVFKLERERPAFAVSGDTVFYVKDRFLRFYEYSTQKEVQVAPIRRPGSVSLNQSPRTLSYSPTENAVLICSDVDGGSYELFIVPKDSAGRADYLQDAKKGSGGSAVFIARNRFAVLEKSSNQVLVKSLKNEIVKKSPLPIATDVIYYAGTGNILCRAEDRVAIFDLQQRLVLGELQASAVKYVIWSNDMESVALLSKHAVVIANKKLVHRCTLHETIRVKSGAWDENGVFIYSTLNHIKYCLPNGDSGIIKTLDVPIYITKVAGNTIAFLDRDGKNKIITVDASEYIFKLALLRKRYDHVMSMIKNSQLCGQAVISYLQQKGFPEVALHFVKDEKTRFNLALESGNIQIAVASAKELDDKDHWYRLGIEALRQGNVGIVEYAYQRTKNFDRLAFLYLLTGYLDKVGFMSKIAGQNNNLMGQFHNALYLGDAKKRVEILENAGQLPLAYVTAATHGLTEIADRLAGELGEDVPSLPEGKTRSLLIPPAPLTSCGDWPLLRVMRGIFEGGLDATGRAEQEEDYDDAGGDWGDEDLEIVDASAVIENGDAGHADESETNEEDGEEEGGWDLEDLELPPEADTPKATGAARSALFVAPTPGMPVSQIWTQKSSLAGEHAAAGNFDTAMRLLSRQLGIKNFAPLKALFLDAHMGSHTFLRAFASAPVIPVAVEKGWSESASPNVRGPPALVFSFAQMDDKLKAAYKATTEGKFPEALRQFLNILYTIPLLVVDSRREVDEVKELIEIVREYVLGLRMEVKRKELKNDATRQQELAAYFTNCKLQKVHMRLVLTSAMGLCFKGGNYATAANFARMLLDNGPNEAQAKKARQVLQACGDRKDAHQLNYDFRNPFVVCGATFVPIYRGQKDVSCPYCASRFVPSVEGQLCSICELSVVGADASGLLCSPTQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GGS7</accession>
    <name>A0A287GGS7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123448738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the senescence regulator S40 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981669.1">
        <property type="nucleotide sequence ID" value="XM_045125734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287GGS7"/>
    <dbReference type="PaxDb" id="4513-MLOC_15499.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0081980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0081980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0081980"/>
    </dbReference>
    <dbReference type="GeneID" id="123448738"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0067190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0067190.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0067190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0081980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0081980.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0081980"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448738"/>
    <dbReference type="eggNOG" id="ENOG502R4Y9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287GGS7"/>
    <dbReference type="OMA" id="PETCHTH"/>
    <dbReference type="OrthoDB" id="688889at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007608">
        <property type="entry name" value="Senescence_reg_S40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33083">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33083:SF71">
        <property type="entry name" value="OS05G0531000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04520">
        <property type="entry name" value="Senescence_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0081980.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="144" mass="15770" checksum="677B0048B4425444" modified="2017-11-22" version="1">MEELDEFEVLWPEYYAGLAHADDDQHKPLPLASSVHAQSAATPWQRRAARSRPVDVPPTPSRAAVLLLRWKDGTDHDDLAGKDGGGKKIIVPPHLLVSGRRLSDGEAAAACTLLRSGAARHGKRARDLRHLRNSVLRMTGFIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MTZ7</accession>
    <name>A0A287MTZ7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123445731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978698.1">
        <property type="nucleotide sequence ID" value="XM_045122763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287MTZ7"/>
    <dbReference type="SMR" id="A0A287MTZ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0330020"/>
    </dbReference>
    <dbReference type="GeneID" id="123445731"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0274870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0274870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0274870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0330020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330020.1"/>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="74" mass="8233" checksum="634B07D77DCC2CD4" modified="2017-11-22" version="1">MAGVDLSKLVKEKRFWAASFLIAWAAALQGHMMWLKRQDAFKDKFGDPEGEAPNKVTEQPAGEQGELTADAEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287MUG1</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287MUG1"/>
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    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330730.1">
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    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330750.1"/>
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    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0330770.1"/>
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    </dbReference>
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    <dbReference type="GeneID" id="123441243"/>
    <dbReference type="GeneID" id="123441247"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0275350.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0330770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="357" mass="39408" checksum="5C71B52D91ED87F8" modified="2017-11-22" version="1">MSGDLVDLSPGQLHKLADLIHRQEVQKLQELEFNSYAEQQKYLRDANDARDKVYHILDSARDMIAQTEAEKDATKQDIGKDVYDYCTKAIGISLQFIRSYNTRLTYLDKLKTHSDDLIKQLKFLNPATQQKEAQRLALEAGMYKKATLENAKKFQHFAPNQFSKWLKENKIMFEDLVQENMSKLGFRGTFKNLDDIQKLQVYDNIIAEAGQGKSVVTYSFEALGKVGVAVLVFTAAAMVWDIYTAEDKLEAAIRDSVNALTAVVNLEVGEIVTTAVEAGFVALDIEIASAAVTVIGGVVGFGIGALIGIAAGALLDLIFSSGTSKVKITDGLTVCRVAPMPHGLELARLVKHNYPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287PU79</accession>
    <name>A0A287PU79_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A287PU79"/>
    <dbReference type="SMR" id="A0A287PU79"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407790.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407790"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
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    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF49">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="6"/>
            <end position="322"/>
        </location>
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        <location>
            <begin position="631"/>
            <end position="779"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="848" mass="96231" checksum="C8A373CA338C2EC7" modified="2017-11-22" version="1">MRPGDSLVTFSSLRTLHIVSANVVSLVESLHQLKHLRYLKLVTDIFVLPVNIGRIKLLQFLDLRGCENLANLPDSVVKLGQLRFLGLADVCMIPRGFRDLTSMRRLLGFRAHMDGDWCSLDELGPLSRLTIIELIQLENVSVALFAANARLGEKIHLTNLSLSCTSKLGDDGLVKEKEGVSAEEQKRIQKVFDELCPPPSVENLRIPGYFGQQLPSWMMSRSRMPLNNLKFLSFYDLACCTQLPNGLCQLPCLQLLQVDRAPCIRHVGTGFLQAESAPFPRLTEMSLEGMVEWEEWEWEEHVEAMPRLEKLWLINCKLRRAPPGLASNTRALKELFVEDVHHLNCLEGFSSVVELTVCGSPDLERITNLPSLQKLTITDCPKLKVLESIPVLERLVLEDYIMEQLPEYMRDIKLRHLQLLCRIWLLSSIAAGQSGLEWDKFSHVEHVKAYTPDGDNQRKWYVLYTRDNFKLDSNITSFTIFSDRLSSFMVEAEGFESVYKMRRSTFSHVCSLVRIPVFEYMMPWDHTFVDGRMLSLQDGVAIALRMLSSGESPVTVGSSLGVNESTILMITRIFVEALWERAYHQFSYPGPAKMEKIKRKFGKIHGLQNCCGVVRTTHITFGSQNCDHEENDGLVMQAVVDPDMRFTNIWLGLPGRMNQSSLLHESGLFMSCDKGAWLNGSKLNLSDGWQVGEYIIGDAGYPLLPWLLTPYHLQDKDSSADFPPYQDEFNRRHSAATDVMLAALARLKDTWKALDREMWRSASSCRLPQIIYACCILHNIVIDLEEEAADMHSDQENYTEQVRQVAEEDAARVRDILSQHLIESGEGKQEIVAVASCSGDKNKEQRAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287Q7U2</accession>
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    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123395391</name>
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    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044946319.1">
        <property type="nucleotide sequence ID" value="XM_045090384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287Q7U2"/>
    <dbReference type="SMR" id="A0A287Q7U2"/>
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        <property type="interactions" value="2356"/>
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    <dbReference type="STRING" id="112509.A0A287Q7U2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0422130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0422130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0422130"/>
    </dbReference>
    <dbReference type="GeneID" id="123395391"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0351230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0351230.1"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0422130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0422130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0422130"/>
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    <dbReference type="KEGG" id="hvg:123395391"/>
    <dbReference type="InParanoid" id="A0A287Q7U2"/>
    <dbReference type="OMA" id="PKNEPQC"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="expression patterns" value="baseline and differential"/>
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    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
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    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
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    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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    <dbReference type="GO" id="GO:0032586">
        <property type="term" value="C:protein storage vacuole membrane"/>
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        <property type="project" value="EnsemblPlants"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
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        <property type="project" value="EnsemblPlants"/>
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    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000687">
        <property type="entry name" value="Ras-related protein RABF2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0422130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="209" mass="22476" checksum="494D9A04DA725B6D" modified="2017-11-22" version="1">MAANAGAGAGGSKIRNAKLVLLGDVGTGKSSLVLRFVKGQFVEFQESTIGAAFFSQTLAVNDETVKFEIWDTAGQERYHSLAPMYYRGAAAAIVVYDITNAASFTRAKKWVQELQAQGNPNTVMALAGNKADMLEARQVPVEEATTYAQENGLFFMETSAKTAINVNDVFYEIAKRLLAGQQAQNPQAGMVLSQRPAERMVSSTSCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287VEE0</accession>
    <name>A0A287VEE0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123410540</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959423.1">
        <property type="nucleotide sequence ID" value="XM_045103488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287VEE0"/>
    <dbReference type="SMR" id="A0A287VEE0"/>
    <dbReference type="STRING" id="112509.A0A287VEE0"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637470"/>
    </dbReference>
    <dbReference type="GeneID" id="123410540"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0529700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0529700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0529700"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0637470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0637470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0637470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410540"/>
    <dbReference type="InParanoid" id="A0A287VEE0"/>
    <dbReference type="OrthoDB" id="629866at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287VEE0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1227">
        <property type="entry name" value="OS11G0462500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="188"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="369"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="569"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="690"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0637470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1045" mass="115746" checksum="BB9688863D8C93CF" modified="2017-11-22" version="1">MDAAIIGALLKTTLPKALSVLGGSRDVRSIQCELRYIEATIQDHRKKCGGKDTSHLRAAWIRDLTLFAFDIEDCVDRFLRQEMSPGDGEAGFLRRSARAAKSTVYTRTVFSFKIRKLKTRSEEIHRRLKRYIEEETAQAAVAPGGAASIPTAGAHPRSGAPAAAVGMDEPLGQLLELVLEPGTSPDGKLRVISVVGFHGLGKTLLAHQVFDHGDVRRRFPLRVWVCAAGKGKPEVLKEMLDQVLQNGAGTSTDSAPQADSAPAGTRSGTTTSNANGVVPQVEENGVEQVENGESTSAGVHVVTTWRHCLQAVWWTLATILLYVLSPAFTVANMVRASSIQVDSADLRRATANASISTSTMALVDENKSVQMKMQVDRGKAIQEDTHKDDADDTTLIEQRLGSYLKNKRYLIVIDGMQTEELCNITSVFPVDNGVNSRVIVTTTIKKIARRCSSANGRVYKMKTLDDNDSRKLFFEVFSKGKYSVADATELKASALLEKCGGLPLALESKAKFLKSEEDLTKSKCEDACRKLCAPEGCDDTLGRMHGVLASSYESLCSLVLQQCLLYFCMFPRGHRVRRNPLIRRWLAEGLVHVQPGDAVSTTPQDIAIENLETLIDRSIIQSMEVSTCGNVKRCQPPGMMLEYIVRKSMSQNFITLLCGESETAEEWDYVRRLSLHDYCAAKLPKGLSRLRTLAVFPAGDAAKNEPTLDFAKYDLLRVLDLEECDGLTDGHLQNICSLVFLKYLSLGGLISEVPKKIARLQWLETLDLRRTKIEKVPVEVIQLPQLKHLHGKFQLIEGDCVKANPEHLSKRSTLETLSGFVMGESEGFPQLMSHMKRLRKVKIWCKSTAKRRNLNQLEEAIKVFIRDGNEWPHRSLSIDFQECSDPFLSSLKAPGSLASLKLRGNLSRFPQFITKLNRLEELCLSSTSLSRGVLLSGGRLDTLKYLKLIEDNIGPLEIRHEHFPSLRRICLVGAQSLHDVATGTLPHLTSLHMICESLDGLAGIEIRRLEGLKEVRLHSAVDQGTKDAWKEAARGHPKRPDVLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287VVT0</accession>
    <name>A0A287VVT0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123413243</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044962120.1">
        <property type="nucleotide sequence ID" value="XM_045106185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287VVT0"/>
    <dbReference type="SMR" id="A0A287VVT0"/>
    <dbReference type="FunCoup" id="A0A287VVT0">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287VVT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657640"/>
    </dbReference>
    <dbReference type="GeneID" id="123413243"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657640.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657640.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657640"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413243"/>
    <dbReference type="InParanoid" id="A0A287VVT0"/>
    <dbReference type="OMA" id="NMGKERI"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287VVT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000018">
        <property type="entry name" value="2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="357" mass="40426" checksum="B9314C0B254198D4" modified="2017-11-22" version="1">MADESWMLPNSVQQLAANMQEPPSQYLLREQELLGENLAGSEIPEPVPTIDLGLLSASGDPEEIAKLQSALQTWGFFQISNHGMETSMMDSVMTTSREFFHLPLEEKKKCSNLIDGKHFQVEGYGNDQVRTQDQRLDWSDRLHLRVEPVGGRNLAHWPTHPKSFRDDLHEYALKCKRIRGDILRAMAKVLELDEDCLVNQFNSNAPTFARFNHFPPCPRPDLVLGIKPHADFPALTVLLMDKDVAGLQYLRDGTWYNVPAARDHTLLVNIGLTMEIMTNGIFTGPMHRVVTNADKERISVAMFYGLDPEQEIGPIAHLLSEEQPARYRKMKAKDLLVLHHEHYAGGRGTRIADTLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W8Z7</accession>
    <name>A0A8I6W8Z7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6W8Z7"/>
    <dbReference type="SMR" id="A0A8I6W8Z7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0080330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0080330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0080330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0097350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF168">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="569" mass="61714" checksum="C4C589E7B24A1E13" modified="2022-05-25" version="1">MGVARPLPPIQSKPDRSSPAMEVASPLLPVQSKPDRSATAMEVARSLPPIQSKPDRSPPAMEVASSPSPLPLPSKPDRSAPAMKVASSPLRVPSKPQRSAPGMEVASSPSPVPVPSKPDRPAPAIEVQVLSSKLVRPAASTDAAVDAGLEYVPLSVFDRVTYQIQMAIIYVFAPPSPSTAALEKGLAAALARYRGFAGQLGEAPGGGPAVLLNDHGARVVEACVDADLVDVAPPMPKPELLLLHPNLEEGMDEVVVLQLTRFRCGSLAVGFTSNHAVTDGRGTSNFLVAWGRATRGVDMGLPPVYNHDELFKSRSVPRVEFDHRNREYYMPVPPLPTKVGSDADGKIKKNIVIHKAHFTKDWIARLRASASEGRGRPFSRFQSILAHLWRATTRARGLRHNETSKIRLSVDGRDRLGVPAEYAGNLVLWAFPQATAGDLLNRPLKYAAQTIHDEVARVADAEYFRSFVDFTSSGVIEEEGLAPSAALNLREVLCPDLEVHSWLTFPFYDLDFGTGTPSYIMPSYFPFEGLIFLMPSYIGDGSVDAFVPVFQHNLEAFKQCVYSIDDLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WB39</accession>
    <name>A0A8I6WB39_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
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        <lineage>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
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        <property type="entry name" value="GLUTENIN, LOW MOLECULAR WEIGHT SUBUNIT"/>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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</entry>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFR7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0045670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0045670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0045670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0045670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0045670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0045670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47127">
        <property type="entry name" value="10A19I.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0045670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="169" mass="18659" checksum="A7DBAA3F6264159E" modified="2022-05-25" version="1">MKLCFANKHATGTYAMATDVPLGTPIVAEHKDKPNVMEGQGTTHEVLQDLPGSNFGLPNASATPDSSPTSNNKRKRASGLTQEDLIECSNMTDAMREIASAVNNTCHAETHPHLYKAVMDLTVFDQKERLIVLDYLTEHKAKGLNFIKMDDEVRQASFKRMLKANSDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WFW0</accession>
    <name>A0A8I6WFW0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFW0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0149360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0149360.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0149360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180670"/>
    </dbReference>
    <dbReference type="OMA" id="ATAWGMD"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF42">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="21551" checksum="955FA24E407D7EF1" modified="2022-05-25" version="1">MAQGECSCSWPSFMKFVGVVVLLFGGTGALLYPLLWPMVSSRSPEYTVAITSVDGLDLRSGRPALSPVFGIALHVDNTREKVVPACVGVDSYAAVSYGDVLLGEGSVPPFCAGIKAVGEVAATAWGMDVHLPHFLRDRLAGELERGQVVVDVAVRTPGGGGCFVNGCLDKVLFCKAKIGGGSPSRCFVANPVSAAPLQKDKINLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WHQ4</accession>
    <name>A0A8I6WHQ4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WHQ4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0046160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0046160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0046160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0046160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0046160.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0046160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0046160.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="28951" checksum="AC1EB4FFAD884629" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYICTVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQSDEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGEMEMLNPVRTHLPAHVDWPAIGGVTSLRGPLLPPVRGPRPHPCPYGSQGSQARVFPGPEVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLR0</accession>
    <name>A0A8I6WLR0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123447534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980079.1">
        <property type="nucleotide sequence ID" value="XM_045124144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLR0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078520"/>
    </dbReference>
    <dbReference type="GeneID" id="123447534"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0063900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0063900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0063900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078520"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447534"/>
    <dbReference type="OrthoDB" id="599181at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF214">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="4">
        <location>
            <begin position="656"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="776" mass="88785" checksum="FFDFB7220068D1E4" modified="2022-05-25" version="1">MQAPADNPGEQAPGPVPATSFEQVAMTSPDRAVEPNHPGAGLRSEKEHVGGGALPEGGLRPTGENHQEHASNGIGTAPQGLLAVILDEQTNSADSKMQQHAEEQDRLHVVTQGHEMSSNDTSSGSDSGSSSGSELDTELGKCFYPSFEELENSRPPEVGMRFPTLEDAERFYSTHAMLTGFAVRRGSNYRRMKFDLECNRSGKLKPTQDLKRKRRCNALGSRCQAKVIVKLHNEQWEFIGVKHEHNHPLCPSPSLASFFLNHKYLSSEEKLFLRVLQQSRVNPRKAMNIFRRMRSNFRKASSSNEKDTSNSKCVDQWRKENSDVETALKRFKELELRNLGFSYTMQKDEDNIVRSLFWTDARSKVDYEIFGDFISFDTTYSTNRHNMPFTPIIGMNSHGRIIVFGCALLQDQKAETFKWMFQTFLDVMGGKLPRIVITDQDEGMATAISEVMPQVRHRFCKFSVMRKTQEILGAFMATRGNINAELHGLVDNSLTEKEFEEGWDVLTERYDASENEYLRLMWGTRKNWVPVYFQADFCPFVESAVHCEGTNLLFKDNVLPKDRIEKFIEQYERMQENIVKAEEEDALHSATEPAYFSLLPIEKHAARMYTRQIFLRAQKELYYSTAFNVHEVQGGSAYRLEKVFDYDNPEFNRNSFEVLAEPGTHAFKCQCAKFTRDGILCCHIFRVFTQLGVKEIPARYMLPRWAPKFKEEQLKEYEEKCSKRRENKMRYTMLLGKMAGISKGICTDGAKSGRLMLELDKVQETLPTTAEENPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WMU0</accession>
    <name>A0A8I6WMU0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WMU0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0083560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0083560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0083560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0101340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF19">
        <property type="entry name" value="CASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035155816" description="CASP-like protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="10">
        <location>
            <begin position="5"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="179" mass="19215" checksum="28C42A138295FA0D" modified="2022-05-25" version="1">MVLPSVVLALRLVAMLLLAGSLALIVADNINVRSDLFEGGRFVLKFKDIYTYRYVVAIAAIGCAYSLVQAPLAAVAIARKKRVIGGTANVALFLICADMIFVIAIATAAGASFWFSIDADRYVDGLYPDSLTKANPDFNRLRQDLDMFFVRASGAAVLMLLAAKCMAAVLVISVYGLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WPB8</accession>
    <name>A0A8I6WPB8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WPB8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0169280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0169280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0169280"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0169280.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="NPH3" evidence="4">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0169280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="308" mass="33951" checksum="3A4B0AD92B0E1D42" modified="2022-05-25" version="1">MRARGMSPESIAGSLAHYARRHLSGLNRRDVGGGTASDTTSSDDVVGEQRVLLEEIVALLPAEKGVATTRFLLGMLRTATILHAGAACRDVLERRAGEQLEEAALEDLLIPNTTYSMDTLYDVDCMQRMLEQFVLSNTTTYADPLPEITADETPPGELMLASTVAKLVDGYLAEVGMDANLKCSQFQQIAALVPDYVRSLEDGLYRAIDNFITAHPWLTESEREQLCWLMNCQKLSLEACTHAAQNERLPLRVVVQVFIFEQLRLRTTVASWFFVGDNNAAAAAQIEPPRAEQPSPAPRARSGEELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WRN4</accession>
    <name>A0A8I6WRN4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965812.1">
        <property type="nucleotide sequence ID" value="XM_045109877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WRN4"/>
    <dbReference type="SMR" id="A0A8I6WRN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0024290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0024290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0024290"/>
    </dbReference>
    <dbReference type="GeneID" id="123426091"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0024290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0024290.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0024290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF20">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3-LIKE CENTRAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22972">
        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="3">
        <location>
            <begin position="23"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="2">
        <location>
            <begin position="173"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0024290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="844" mass="94879" checksum="50E07F3A92C616C0" modified="2022-05-25" version="1">MGEKPGQGRKEAWQCESLRTRMKRVKVYRLEDNGKWDDQGTGHVTIDYIEGSKDLALAVVDEEDNDTLLLHNITPDDIYRKQEETIISWRDPEKALELALSFQEAESCSFIWENMCTIQLELQSKILGSHEVRPQRALKSLEAPRDSLSRGKSLAFELPPLELSSLSVILETILQCDVAQQTHVADLILKDRDFFPKIVNLFRTCKGLGDMGGLHIIFRLVKAIILLNSAAIFDKIFSDDFILDIIGVLEYDPEVCNVQNHSAFLKEHAGFKEVIPIRNASVVSKIHQTYRICYIKDVILPKLDEATLASLNAIINANNAFVVCLLKDDTSFMQRLFATMRSSNISSESKKELVLFLREFCTISSSLQPAQRMQLSRDLASRGVFDIISDVLRSQEKVLVSAGIDILHYLTQDPNLLRSYIANHEENSREGISLLGLLIEGILTDFGGEMPCQFLEILKVLLDGCTADTVTQCRDFIELFYEKCFDKLIDIIESSRVEEYSAKPEILYNICELLCFCARHHPYKIKIAFFGSNSMEKILTLTRRRERSLVVAAVRIMRTIIGAGRNDELLISHVIEYNTLKPIIEVFVENGNRYNMLHSTVLELLDFIRKENLKSLVVYVVESFWDQLLMFGQLTSIQAFKLKYQEYVDIAEEMQTTSVDHTRNADERGLDKEEEDYFSKDSEEDSAAHTIHAHQQCLVKPANGSEIHHIPARPKSGGLVDNGVDKDYKSSPKRPVKADEALNTPMASYSSLDSKDADGNILKKPKLEDKQPPLSPASSIKNSDGNDDVGKASPGSPDQQQHALESLDSGHQTGDDCMKDAGDSSPEMALDTAKPTDSEPSTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSJ7</accession>
    <name>A0A8I6WSJ7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123444085</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044976632.1">
        <property type="nucleotide sequence ID" value="XM_045120697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSJ7"/>
    <dbReference type="SMR" id="A0A8I6WSJ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279390"/>
    </dbReference>
    <dbReference type="GeneID" id="123444085"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0231540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0231540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0231540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279390.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279390.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279390"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444085"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF821">
        <property type="entry name" value="ATP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="541"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="344"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="451"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279390.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="796" mass="88607" checksum="545117CB87E38AAD" modified="2022-05-25" version="1">MDETPTSSGRSEATSCEPSWWPPDFLEKIESVALAREQEVLAEKESRFNLSNSKSSSWKASQLLWSTGTYSGFIPNGFYSIIPDKKLKEIFPTIPSLNDLQSLEADGLKPEIIVVDAEKDKKIFMLKQLSGALVKGLNNPALVIKKIAGLVFDCFKGQNSDASPGRASTEDIHFFGNRGPQLLGQIRHGSCRPRAILFKVLADAVGLESKLVVGLPDDGAVGFVDSYKHMSVVVPLNSMELLVDLMRFPGQLIPFSAKAIFISHISAAGESDSAENDSCDSPLEPNSPLYGLSDKVEAEGTEASSNLSGRSLRNTMLRSRTFSEGKLSTSCSEPNIANAFWRRSQRRGVAEEPRGASSSPEHPLMRAKGRSILGGDRQSFQEYTDRVTLRSDDQGATSTPNPRRIRRRSISITPEIGDDIVRAVRAMNETLKQNRLQRDHVNDGSCSFIGEDKTNANDCPNNDDKSGTNNGLRNRAGSTQKAMSLPTSPHDYGGEISETNNNCDFISEEKMVFAWNKVLQSSPFNKPLSPFQEWNIDFSELTIGTRVGIGFFGEVFRGIWNGTDVAIKVFLEQDLTTENMEDFCNEIYILSRLRHPNVILFLGACMVPPHLSMVTEYMEMGSLYYLIHMSGQKKKLSWRRRLKIIRDICRGLMCIHRMKIVHRDLKSANCLVNKYWTVKICDFGLSRAMTDSPMTDNSSAGTPEWMAPELIRNEPFSEKCDIFSLGVIMWELCTLRRPWDGIAPVQVVYAVTEGSGLEIPEGPLGKLIADCWAEPQDRPSCQEILTRLLDCEYAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WYF0</accession>
    <name>A0A8I6WYF0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123447619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044980162.1">
        <property type="nucleotide sequence ID" value="XM_045124227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WYF0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338620.1">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="255" mass="27044" checksum="C81940EFA61BA5BB" modified="2022-05-25" version="1">MCRAPVDDLGRLAPRPRTRRLDVRAFYLRLSSSSPTSTPPAELTLLYRPAIGGAALELAGRALPPASPAEVALARVRGAKDDDAPAYASADRVSAAEGARFEVYDGKELAAEGAFFFARGLDGWRVECRRPAASRSRVAEVLVLAKGGALIRARARAARRAGLGCSATPLEGIPEEGPCGCQCGACGDDDWEMVGDSSDDDADADAGHGLKEEEEVDADSMRWALEMGAWAVCVGVGLLATARRFSRSRRRAALR</sequence>
</entry>
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        <name evidence="4" type="primary">LOC123395632</name>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="gene ID" value="HORVU.MOREX.r2.1HG0006380"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0008630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0008630.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        <location>
            <begin position="185"/>
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        </location>
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        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0008630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="31747" checksum="C0D469E0EF0F17A9" modified="2022-05-25" version="1">MEVFDDVHFVRLRCRVRRTKYLAADDDGHGVCLSSQRGAHNTVWAVQHMEGAVEGAEGGPFVLLRGAYGRYLFATDVQANTGPGHGVQASQQPRPHPNTPRCMLWQAIRREPSFIIRNAGGRYLRANGKYLRWRTAATVAGDDGSAMMQWDVQAVPLRLDRPTLIDPPPQLMRRRRRPPTEEDVSRQIRYIRAGVDGDIDETGWRTVRISTNSLMQLRLTLANLLGQNRSALHTTLCVRAGAYADLSPLLIDLPIGNDRLDIVVITHGTPVDNSLLYPNVNARN</sequence>
</entry>
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    <name>A0A8I6WZH9_HORVV</name>
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        <recommendedName>
            <fullName evidence="9">Myb-like domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="7" type="primary">LOC123440068</name>
    </gene>
    <organism evidence="7 8">
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        <lineage>
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            <taxon>Pooideae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044972593.1">
        <property type="nucleotide sequence ID" value="XM_045116658.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6WZH9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277710.1.CDS1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123440068"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0230080.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0230080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277710.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440068"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
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        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044636">
        <property type="entry name" value="RADIALIS-like"/>
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    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF105">
        <property type="entry name" value="OS01G0663051 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="12"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277710.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9464" checksum="D9E571D40B905D0A" modified="2022-05-25" version="1">MSSGSTSSSRGGANAVWSVRENKLFEEALANYGEGTPDRWHKVSRAMGGTKTADEVRNHYEILEEDIKLIESGRVPFPKYKYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZM7</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="2">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044965689.1">
        <property type="nucleotide sequence ID" value="XM_045109754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZM7"/>
    <dbReference type="SMR" id="A0A8I6WZM7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134190"/>
    </dbReference>
    <dbReference type="GeneID" id="123425979"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0110590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0110590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0110590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0134190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0134190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0134190"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425979"/>
    <dbReference type="OMA" id="RVTKPCD"/>
    <dbReference type="OrthoDB" id="6078042at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF131">
        <property type="entry name" value="OS07G0566800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="2">
        <location>
            <begin position="52"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0134190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="224" mass="25015" checksum="7F66BDD819E3506E" modified="2022-05-25" version="1">MASTGVSRRSSIMASRLSASAEAVRASEGRKKQQAVVARRVEYDEESLAGEPQYDVFINHRGADTKRTVARLLYDRLAQGGIRGFLDNMSMRPGDRLRDTIRAGIRECSIAVAIFSPSYCDSEYCLWELAMLVESRKTIIPIFYNIKPSDLVLPEAVAASEGYLPEDVERYKYALREAKNTVGLTYDSATGDMAELVSAAADAVLYNIEKMERVQRRETIVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X0W7</accession>
    <name>A0A8I6X0W7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">F-ATPase gamma subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123425909</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has four main subunits: a, b, b' and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965610.1">
        <property type="nucleotide sequence ID" value="XM_045109675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X0W7"/>
    <dbReference type="SMR" id="A0A8I6X0W7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141490"/>
    </dbReference>
    <dbReference type="GeneID" id="123425909"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0116510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0116510.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0116510"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0141490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0141490.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0141490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425909"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000006">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000003">
        <property type="entry name" value="ATP synthase gamma chain, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000004">
        <property type="entry name" value="ATP synthase gamma chain, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004145">
        <property type="entry name" value="PRK05621.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF41">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0141490.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="357" mass="39706" checksum="C037151C063F970B" modified="2022-05-25" version="1">MSCSHLSTAWSSSALASTSTRRRASTGSSLVVRCSLRDLRNRIDSVRNTQKITEAMKLVAAAKVRRAQEAVVSSRPFSEALVEVLYNMNQEIQSEDIDLPLTRQRAVKRVAIVVLTGERGLCGAFNNNVLKKAEARMEDLRQLGVEYKVISVGKKGNAYFQRRDYIPTERFLELAGIPTVKDSQAICDLIYSLFVAEEVDKVELVYSKFVNLVRSDPIIQTLLPMSPKGEICDVNGICVDATEDELFKLTTKEGKLTVEREKIKIEMQPFSPVVQFEQDPVQILDALLPLYLNSQILRALQESLASELAARMSAMSSATDNAIDLRKNLSMVYNRRRQAKITGEILEIVAGADALSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X4W1</accession>
    <name>A0A8I6X4W1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X4W1"/>
    <dbReference type="SMR" id="A0A8I6X4W1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0055690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0055690.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0055690"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0069320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0069320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0069320"/>
    </dbReference>
    <dbReference type="OMA" id="GMEENFQ"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000006">
        <property type="entry name" value="Agamous-like MADS-box protein AGL62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF629">
        <property type="entry name" value="OS02G0164450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0069320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="432" mass="45256" checksum="5F6A127DF3DC4EC3" modified="2022-05-25" version="1">MAGRRGPAGKGRKKVVIQPIENQAARDVCFSKRRKGLFSKASQLAVMCGAEVAAVVYSPAGNAYSFGHPSAEDIINRFLGTGGQDGAAGARQLGAAVDRNRLAELQQEYNKLGKELAAMDERKESWGEAMAKDRAEGSQAAAWLDAVADRRDMGDADKLACAASLREAQVAVSARVNQVLQDGRREQQAALAARVNQVLQAQKAPLGPAALPPQLLAGGGGFELGGTSVNGWTEQMIQQEQIMTVMPTPGFAGGVGFELGGTSANGVMEEMMSAIASGGGFEFGGTSANDGMEENFQQQQMVTAMPQLPGLLAGGGGFELGGTSADGGMYEMMMTAMPPPLGFATGMEMMQQQEQQMMPSGMMPTPPGLAAGMEMQQQQTMTEPPPLPAFAAGTEMMQQQEMMAATPPPAPGFTAGMEMMHQESGPYGSFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X6C0</accession>
    <name>A0A8I6X6C0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123445275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044978175.1">
        <property type="nucleotide sequence ID" value="XM_045122240.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6X6C0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311470"/>
    </dbReference>
    <dbReference type="GeneID" id="123445275"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0311470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0311470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0311470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="CDD" id="cd00072">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46992">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46992:SF1">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="496"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="866"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1363"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="867"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1377"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1418"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1439"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0311470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1513" mass="167635" checksum="F09CA5F34E1B17E5" modified="2022-05-25" version="1">MAERKLDRFAALGKDALSLGIDEDRSTAAAMGFVDDPKDQQHLENSIPLSPQWLYAKPSDAKISAPHGSLLEPSEKDVRMLEGSGAKKERRRNAFDAGWLDEERETSLLGRRDHKKEVDREVENRKNDRRSDNVSARDNNDSRPAPTSGRWDDGSTRNSGNEGRRDGKWSLRWGPDDKEKDSKPEKKMDAEKDEPHGEKHTLPVRLLPESDSRDKWRPRHRQEPQTGGTATYRVAPGFGLEKGRVKESNVGFAPGRGRANPNSVPSFSRPSSAGPIGAPPVYGRRAASAGAFRYPRGKLLDIYRQQKVVQSFEDGRRMLEEVPPITLSSSVKPLAFVTPDNNEEAVLEDIRKGRVTSSEATNSTASQKERNKDLEAFGIDANKDKSAEAFSGLSQEGSAALISEKDAFYNDGMLTGGITGPPKKPADENAPSHPHGLSGMRDDTQFNEVKSSADIDPSTKLPDDSNAQFNVNVEYPTGQASYTETKAGGQESYPEELTLYYLDPQGGVQGPFMGTDIVSWYEDGYFGLELPVRLAQAPDDAPFRPLSDLMPYLGHKPQSLPPVSRGGSAESPDSVHNNFEDALPTSGSFGKSDQTTKADSGNYAANPTRGDQEAPLQSHTSWFPSVEAQKTEANPDIRQQRIPETVSHDAEEVLYTGRPTSGMGQSRPDFDNDRADFQLASVDSRSGVREANLPKQDAPRENELSPLGLLWSELEGMHPKQPLSSNVLGVNERRNPKQTAPKDIPPANIRHGPLSRMNEAPAVRDEWSANLGRLDSINDANMSGLISQVEPELGHLNYEEQMLLTQIRREQLQQEQMMGRNNLEFPGQFAGQVFDSLHQHRQSINPPAPEVEHLLRVQFELEHQQRRQQLQQEQHRQQLQQEQHQRQLQQEQHQRQLQQRQAQLLQQQQQQQQQQLIIEQMLQQQLQSSNFGQANMVDQVLLREQLLNDLHHQPHHFQRQHDAAIEQLIQAKFGHGHHREQHNDMLDVLSRSNQRQALPLEQQILLGLHHDQLQTQQLANAIRQHAGREEERHLGGGWPMDDPSQFIRTGTSPNQSHTSRLGQFDLLQSLQRSSSVEHHDHLDRSLSMHERLHRGGQGIHSLERSGSLPGGAPLPNPDVVNALARHHGLGQMETHGDLYSAGQMPMHASGGVHPQQHRLQEQLSGSHMGRLERNWSDANGQLQNSLMEASRINQMQIEAEKQRRNVEMNLPIDNPHAWASLMNNERNPEAELSDMIHQKLVLQAQQSRGFPDVPPTASFGQRSHFSKMGHLGQDGPTSLDILPNNIEMNRKLGVRSSSATMLDMQRGEFPDVMGGSVSTNQLVGNANDVARRKRQGSSANLAVEDTDFSEAVSNWVDTGISKGSSHSLLKRTANPHTATSQAQAASTDLSSTIRSKKAGHASSASSDEHKMESGVTSAAHGVEAAASANKEAGTFGIPPSSNQDASGPSFSEMLKSTKKPPLQYDASESADGGPGGKGTKKKAKKGKQIDPSLLGFKVHSNRILMGEIHRPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X727</accession>
    <name>A0A8I6X727_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6X727"/>
    <dbReference type="SMR" id="A0A8I6X727"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0087800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0087800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0087800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0087800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="22002" checksum="DB7DE91FCA783054" modified="2022-05-25" version="1">MEFFTVILEKSSRLVLPHNFVKMLDGHRPQNLKPRQAGNGLRKLWDVEVVFDADGRMYLDRGWKQFLRAYDLGIGYFLVFRYDGNATFVVKVFDTTMCWRRYQDDDDAGNGSRSSEYCDRCYVYGSNDSGSKSSNDSGDSKSSSDDGLAMDVPQLGNRAMPIVVEEYIPQPGLGLRRSKHIKMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="primary">LOC123429926</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045113894.1"/>
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    <dbReference type="SMR" id="A0A8I6X772"/>
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    </dbReference>
    <dbReference type="GeneID" id="123429926"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0114990.1"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="300"/>
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            <end position="324"/>
        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0139560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="362" mass="39102" checksum="365B733A7A5A36C3" modified="2022-05-25" version="1">MGGANGAKAYGAVVLIRVMYSGMHIMSKIALDQGMNPFVFVFYRHTTAALVLIPIAFALERQKTKPVTLKIAGKMFVHALYGVTACGVLFNLGLNYASATSSSALYNVQPVVTFILAVIIGMESMKLKKLHGNLKVAGILCCVAGVTVLAFYEGPMFRSFNHHRLFQHGSGSSSGAGTYSKNQWVFGIFLMTLSNILAGLWTVLQGPLIEDTSKLMNTTLQISCASVQAFVVAVAAERDFSKWKIGWNISLAAIIYSGVIVTALSYYMQMWTIAKRGPVFLAMSMPLTLIFTIIISSFVLGETVSLGSIIAGVLLIGGLYNVLWGKSMERKDETNKMGGGKPAMELQDKEQPQVPDDAAAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCH2</accession>
    <name>A0A8I6XCH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123427836</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCH2"/>
    <dbReference type="SMR" id="A0A8I6XCH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196020"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0162660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0162660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0162660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0196020.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0196020.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0196020"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Brevis_radix_dom"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF350">
        <property type="entry name" value="F12P19.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08381">
        <property type="entry name" value="BRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="407"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="458"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="510"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="565"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="627"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="679"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="731"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="8">
        <location>
            <begin position="787"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="BRX" evidence="9">
        <location>
            <begin position="1103"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0196020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1170" mass="128891" checksum="C0D9EF15824B0AD3" modified="2022-05-25" version="1">MTTLLVCLRLTFCRRLHVPKKTRTRLSSCVPDKKPNCRCRNASLSLSRFTFAAAPPATARGWRHSSPFSNHCPIISPPFLALFHSSSAPARPLSSQLHFHSPHCNLWHTTVHHLRANASFRLSDDPTNPSSCHPSACLICPHPAAMAGSFDGRMPTRGVEQAIVALKKGAHLLKCGKRGKPKFCPFRLSSDEKTLVWYSRDREKHLSLNSVSIVVLGQKTTNFHRQRWPEKEHQSLSLVYRNGECSLDLICRDRDQAECWHLGLTALISGSSSPFSSVGSKSSRQTTSCTTTPRSYIQRKSKLSAVHDTPRHKQVYPSYGSPKMTQKDLFGGYLDCSEALFYQRQRTFSDVDSYLERLSPKVANPVRHSLKDILVANNKHKTPTISRTPKVKAFEGPRAACRLDSLKDVFLWGDIVGSTLDSEDIPKALPRLVGSTKMLDVQSIACGQNHAAIITKQGEVFSWGKEGSGRLGHKVNDSAPCPKMVESLASVHVKAVAFGSKHTCAVTVSGELFEWGEGAHSLGLLNNWHGRNQWLPHKLFGPMDNISVSKIACGDWHTAIMTSSGQLFTYGDGTFGVLGHGNTQGIARPKEVESLKGLRVKSVACGPWHTAAIVEGMSSFKCNAPIGNLFTWGDADKGKLGHADKKMKLVPTCVDSLISHDFLQVSCGMALTVVLTSNGVVFTIGSSKHGKLGNPQADGESVCTVGGVLKNEFVTEISSGSSHVAVLTMNGKVFTWGKGADGQLGLGDYANRSSPTLVEALEGRQVQSIACGSNFSAAICLHKGISVKDQPICSRCQMVFNFTRKKHNCYNCGSMFCNSCSSNKTANAALAPDRNRRYRVCDACFCQLEKVLYSGEVKPQPKISKGEMFKAEIMAYTPKLSRLFKEANLIVEKMASVQGHNQRNMDSVDPVQVKTQRWGQVECPAKFLSAQDNFRHQLVPNNQMSGVSFSQRMHDAVGLKAGNSLRQSTDSQRDEMSMMERILTEEVKQLRSQVTALAEQCQQKNLVVQVYKQRLDETWLLVRDEAAKCKAAKDIIKVLTNQCNALSAKLSVGQQPENLKITPNSIPEQAPSPGGSFSHRNIARTFDRDGYTTEADSVDTPVEGVIEQIERGVYVTLAVSSSGKKDIRRIRFSRKHFGEKAAQCWWEENKSRVYANYSVEQAATPHPNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCT9</accession>
    <name>A0A8I6XCT9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123445331</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978234.1">
        <property type="nucleotide sequence ID" value="XM_045122299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCT9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0312690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0312690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0312690"/>
    </dbReference>
    <dbReference type="GeneID" id="123445331"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0261230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0261230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0261230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0312690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0312690.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0312690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445331"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF94">
        <property type="entry name" value="POTASSIUM TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="12">
        <location>
            <begin position="65"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="13">
        <location>
            <begin position="565"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0312690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="784" mass="86856" checksum="BDBA8054C136DC5E" modified="2022-05-25" version="1">MAEPLKTNGNGAAEGGSAFASVKVPPPPSPPRRLQRFDSLHMEAGMIPGGHSYAAKVGWPTTLHLAFQSLGVVYGDMGTSPLYVFSSTFTGGIKDTDDLLGVMSLIIYTVLLLPLMKYCFIVLRANDNGDGGTFALYSLISRYARISLIPNQQAEDATVSHYKLESPTNRVKRAHWIKEKMENSPKFKVILFLVTILATSMVIGDGVLTPCISVLSAVTGIKQSAKSLTQGQIAGIAIGILIVLFLVQRFGTDKVGYTFGPVIFIWFILIAGIGIYNLITHDTGILKAFNPKYIVEYFQRNGKDGWISLGGVILCITGTEAMFADLGHFNVRAIQIGFSAVLLPSVLLAYMGQAAYLRIYPEDVADTFYKSLPGPLYWPTFVVAVAAAIIASQAMISGAFAIIAQSQVLGCFPRVRVTHTSKKYHGQVYIPEINYALMILCVAVTAIFQTTDKIGNAYGIAVVFVMFITTLLVTLVMAMIWKTSLLWIALFPIIFGGAELLYLSSAFYKFVEGGYLPLGFAAILMLIMGTWHYVHVHRYKYELKNKVSNNYVAELATRRNLARLPGIGVLYSELVQGIPPILPHLVEKVPSIHSVLVITSIKFLPISNIETNERFLFRYVEPREYRVFRCVVRYGYNNKVEDPREFENLLIGNLKQFIHQESLYSESSHSLAGEDNAMEESGDAMEPSVEVQDARLPKRFVDGITASPVNGCMDEIEFIQRGMDDGVVHLLGETNVVAEQNAGLVKKIIVDYAYNFMRKNFRQPEKITCVPHNRLLRVGMTYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XCY7</accession>
    <name>A0A8I6XCY7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XCY7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0384550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0384550.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0384550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0384550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0384550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0384550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="192" mass="20864" checksum="464B348196EE642C" modified="2022-05-25" version="1">MWHYTGPEDSTRTNVVGVTEERVTSWVLQITGPCENPKGARGVKPYSADHPPPNQAWTNWISPISNGNPEEEEEEEGSQEGSVESAEYVSDSKESEEEDSEEEEEDEEQDSPPPRPEHRTKRRHEPAVPSAPPASSSAPPTAPVVPSVRGTKRARDAAAEPAGQSSRAPKPSGPKPRKALLQMRVAIPVTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="6">Trafficking protein particle complex subunit</fullName>
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        <name evidence="8" type="primary">LOC123448259</name>
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    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XE04"/>
    <dbReference type="SMR" id="A0A8I6XE04"/>
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    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0298620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0298620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0357960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0357960.1"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="TRAPPC1_MUM2"/>
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        <property type="entry name" value="Trafficking particle complex subunit 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
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        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <end position="34"/>
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            <begin position="10"/>
            <end position="19"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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    </evidence>
    <sequence length="219" mass="24269" checksum="9534C5CBC2850832" modified="2022-05-25" version="1">MCWPIISMPYRSTRSSPATTPFPRRRSTPYRGAAPPASAAAMQFFSGSSLTSVAPESSPAPAAPPGTGTGSNAQVIYVFNRNGVCLLYREWHRPLRTLARNQDQKLMFGLLFSLRSFTAKIDPTSTEHANLGAPLLPGQGCSFHSFKTNTYKLNYMESPSGIKLILLTHPRTGDQRDALKHIYSLYVEYVVKNPLYAPGSPIKCDLFNKHLDQYVKTLI</sequence>
</entry>
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            <fullName evidence="4">Telomerase Cajal body protein 1</fullName>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045124352.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6XFE9"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0340710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0340710"/>
    </dbReference>
    <dbReference type="GeneID" id="123447717"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0284400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0284400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0340710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0340710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0340710"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447717"/>
    <dbReference type="OMA" id="IRTWILP"/>
    <dbReference type="OrthoDB" id="239865at2759"/>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="SWT21/TCAB1_mRNA_Telomere"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0340710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="46845" checksum="ADECE90641C00C3C" modified="2022-05-25" version="1">MEEAAEEQAAAPAAAEFGDATENGTEEEGASAAAAAAEEAAAAEAAAAATAAAYSWPELRFDLRPRRRYHFADQFRSPCSSSAGNFLKGVKWSPDGSSFLTSSDDNSLRMFYLPEDAYGAAAEDTAEAAVGGQDSYGASLQVNEGEPVYDFCWYPYMSLSDPATCVFASTSRDHPIHLWDATTGELRCTYRAYDAMDEITAALSISFNSTGSKLFAGYNKAIRVFDVHRPGRDFEQYSLLKGGEGPTGIVSSISFSPQNGMLAVGSYSQTTAVYAEGNMEPLYVLHGQLGGVTQVLFSKDGNYLYTGGRKDPYILCWDIRNTVEIVYKLYRSCDTTNQRVQFDIEPCGKHLATGGQDGMVHIYDLQGGQWVTGFQAAADTVNGFSFHPYLPLATTSSGHRRFGMQDEFEEESSLAGDENCCSVWKFSCSQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XGJ1</accession>
    <name>A0A8I6XGJ1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123449021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">subsp. vulgare</strain>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </citation>
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        <location>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="998" mass="106141" checksum="19FB318C0D62963F" modified="2022-05-25" version="1">MVATLCSTALLLVVVALLSASGDGAAALDAQAAYLSRLKQELAGPAMARWDFSSATPVDYCRFSGVVCDGRDGNVTGIDLASWRLAGRLPPGICAALPALRDLVLAFNDIRGGFPAGLLNCSSLETLNLSFAAVSGALPDLSPMRALRVLDLSDNLFSGAFPAASLAGMASLEVINFNENPGFDVWRPPEALTRLRRLRVLILSTTSMRGGVPAWLGNMTSLTDLELSGNSLTGRIPASLGRLANLEFLELYYNDLEGAIPDELGNLTRLADVDFSENRLDGAIPDSFCALPRLRVLQLYTNFLTGPIPAVLGNSTQLEILSVYKNQLTGELPADLGRYSDFNVLEVSENQLTGPLPPHACANGKLQYILVLSNLLTGTIPAAYADCLPLLRFRVSNNHLEGDVPPGIFALPHASIVDLSYNHFTGSVPPSIAGAKNLTSLFASANRLSGMLPPEIADVWGLVKIDLSNNLIAGPIPAAMGRLVRLNQLSLQGNRLDGPIPETLADLRSLNVLNLSENALSGQIPEALCALLPNSLDFAGNNLSGPVPPAMIKEGLLESVAGNPGLCVAFRLNLSDTALPLCQKGGEKRGFAGSAWIAGVCCVVCVVAMLALARRWVTRRWAAKDGEQDGTSSPGSRGGGGSSYDVTSFHKLNFDQHEIVEALIDKNIVGHGGSGTVYKIELSSGELVAVKKLWVSSSKQLRQKQQQHRGHGRGYGGGGGGEEDDSRELRTEVETLGSIRHKNIVKLYCCYSGADCNLLVYEYMPNGNLWDALHGGAGGGWGGFGFLDWPTRRRVAIGVAQGLAYLHHDLMFAIVHRDVKSSNILLDADFEPKVADFGIAKVLQATRGGVGVGGDTSSTTTIAGTYGYLAPEYAYSSKATTKCDVYSFGVVLMELATGKKPIEPEFGETRDIVQWVTGKVAGAAEAEALDKRLAWSPFKDEMVQAMRVAVRCTCSIPGLRPTMADVVQMLAESGPPPGSRSAKDFSGHKPKPADNEAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHB0</accession>
    <name>A0A8I6XHB0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123442354</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHB0"/>
    <dbReference type="SMR" id="A0A8I6XHB0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF76">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="511"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="554"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="735" mass="82814" checksum="4B1CE8A75FB6B926" modified="2022-05-25" version="1">MEKGSLSRHLSAESTGHDWHTRYAMIKGICNGLKYLHEGVKPPIFHLDLKPANILLDQNMIPKIADFGLSRFFNEEKSYSTNDLIGTLGYLPPECIPHGIISNKFDIFSLGVVVIKIIAGPEGYSRSAETTSDQFTETVHDNWRDRLQLTASGDLLESYSEQVKTCIEIALSCLKADRQERPTIGDIVNKLTGTETPENYHEALIDEVRSTIALSCLEDDRQKRPTRGDIVNKLTGTGTLENYDEAKWQKRLLIDNKFTGRETIKNYLEALIDEMSTNLIDVHPLQLHFSFRPNKCISCVFHVTNNMNDQHLSVRCVPKHEEATPRYYACRSRIACVVPPRSTHTFVVTMVQQEQPPAIMDVLDMVVETILGEVKLRSLSDSFDKHFEDAREEKSCGFREVTLKAVCATTSKTTTSEVLMGDDRLWSMDVHPTEPWILAGYARRLVIWNYKTQTRMMECGFFPGRADASYSVVVNTVRFIAKSKEQLFVVGDDYGYIYVHDSMTMKELKKFKAHGSSVTSFAVHPTQPFVLSASWDKLIKLWDYDKGWECIQTFTGHSHWVWQVKFNPHDAANTFASASGDGTVKIWSISSPMHVTSLDCEGGQGCVDYYPTAAGGDQQHYMVAGSIYSGKTSIWDLRTQTCIREIKGLQSLCEVGVIDCHPDRPTLLLTASEDYGVSLVDSTTYRCEKTIHFGLDEVRCFAYIKSIRSLAIAHHFGIAIVGIDEFSKDGLPPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHE9</accession>
    <name>A0A8I6XHE9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHE9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0423840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0423840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0423840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF48">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="505"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0423840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="69003" checksum="8750B235CCDCEDCD" modified="2022-05-25" version="1">MKLVGWNCRGLGNGPAVQGLLEVGRTEDPDIIFLSETKLTEKELEKFKWKLGLCNMVARSSEGRSRGLALFWRRGVNISLRSYGRRHIDVDVTEDDGTLWRLTGMYGESEMERKIETWRTIRLLGQQHEAGRPWMCYGDFNEVLVDDEKIGGGRRPQAYMDRFRDSLVHAGLCDIGYTGDKFTWRNHNKEIHSYICERLDRVTANAEWCGKFPGFSVVHERPRHSDHRPLVINTMGDAGRSGGGGGDRGFRFEAWWLQEEGCAEVIQEAWEEGKAEGCSVAQSMRKVACRMRRWHKEEIGELAGRLKRAQTDLERCMCAPVSEAKIREEGRLRGVVNDLEEKKNTRAKQRSHVAWLKDGNRNTRYFMAVASARRKKNRIKGLVKEDGTVVKEGNDLTNYVCSYFQGLFTSSMSGRLADLLDTVPARVTNDMNNIMSADFTCEEVKAALDHIGDLKAPGPDGMPSVVYKRNWPLMGDQVVEEVLAVLNGGEIPAGWNDTVIVLIPKVKSPSRIKDLRPISLCNVLYKLVSKVIANRMKLILPQIISDNQSAFIPGRLITDNVLISYEISDYILHKTKGKEGYAAVKADMSKAYDRVEWSYLDAMLRRLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIX0</accession>
    <name>A0A8I6XIX0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0346640.1.CDS.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 4H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="OS12G0134500 PROTEIN"/>
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    </dbReference>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0415900.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="150" mass="16476" checksum="CD81B021A13C0AAF" modified="2022-05-25" version="1">MDRPQLTIVAPEYVVDVDDLVIEARSAGRQRTAFFGTGYNSPHEGAGCLFSAAASSRAIQGLHAPAVGETRELGCAVCLDDFKDGDELRSMPCSHSFHQRCIFRWLQISRLCPYCRFALPSVHEQRVPDLDLYLDEQAAQAGSGSEASDR</sequence>
</entry>
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    <accession>A0A8I6XNT0</accession>
    <name>A0A8I6XNT0_HORVV</name>
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        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="primary">LOC123444296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_045120978.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8I6XNT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285480"/>
    </dbReference>
    <dbReference type="GeneID" id="123444296"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0236870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0236870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0236870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0285480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285480"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="REXO1-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315:SF26">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NRDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="689"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="980"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1025"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="1224"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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            <end position="1038"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
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            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="1147"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1172"/>
            <end position="1196"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1298" mass="141641" checksum="BFB498B86E9466BD" modified="2022-05-25" version="1">MSMQMDVDGQPSEAQEAVLESYTSENEICGICRDIVIDRGVLDGCQHWFCYTCIDNWSAITNRCPLCKIEFQHITSTPVYDSTGTGDSIEVEDDYPLTSGDDDWYEPGESNPLSFPSYYIDAEAVVCLDGGDCIIRSGLVASEDYSALDTSIACDSCDLWYHAICVGFNPEITSEDSWLCPRCVSTEVENKSDVALKQNISGDSDRTSTDASFSGRVSVSVADDGETALVVSMVAVNSESKDDLLEGSLGSKTAQEAFYCNSLPSYSKDDLSSEAVANACIPRNKDSSCKPHNKSSEINLACLVSSEPTQRSSDHSPIRESACIPFSSEHGNISNEQSDGLQHGPSYSSLCRSMEAETTGEEAALPRNSNEKSPVIKSAQLSSAASMSATSSGIDMINTDAVRKRKDDKNTQLPPMQDRHNISDMELQSEISHPAKKARLEVQDQEMHLIANSGVSSSDCHATSIATNFIASDTSKIVTQNKYVPDIMSIVEGESYMRDPGRELAKPVGRRAGDKPGLRMKKILHKDGKESTAVVQKLQQEIREVVRDNGISILEKDNAFDEKLLTAFRAAIGKSMDGPDKRTNLSLARKSVLQKGKIRENLIKKLYASSTGRSRSAWHRDREVDFWKHRCSPGINPEKIETLQSVLQLLKKSSDTGTRKETAEEKKAFLSRLYLADASVVPRKGDIKPLSALEGCPPFDKNSQIKGDDSKSTNKPSPVTQTIKINSPNSTGKVLSSSTLSKEASSRRENKNGQASQNKPNQSAGDIKLDKRKWALEILARKNASSIASKDQTEGTDDLSTNYPLLAKLPVDMRPQLTAGRHNKVPMSVRQAQLYRISEHYLQRANLDVIRRCADTELAIADAINVENDIYGKSSSKSVYVNLCSQATRQSAKAKPENDASVLTEKAEVSSDLISQHVTTESTYSASSNVEEDLDRAGLLDIPATAGQTDKSELGGVPEQNASENAVCFNSVEEALKRAGLFDSPPNSPERKSTTAEGNSRTVSGSPISPRQKYINAEDTSLVNLDSEPSISLQSTSDSRVRDAPHLKDDDSSKQVLYDTNCQNLDTTTSCQQPKCNSEEGQKLIHGGETTNATSNETLPVNLAEADKCNLQCEKTSQTEKETVADMPDEGTGHVENSKEVDITVSDLHNQSSHRNNVPKEGEGISQAMKLEPGKEKSSSGNEELNRKHSKADKSSTHPAESVDSLKKPAPDPGNNSASDSSSSIHKKVEMFVKENIRPLCKSGVITVEQYRWAVAKTAEKVMKHHSEAKNANFLIKEGDKVKKLALQYVEAAQQKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XNU3</accession>
    <name>A0A8I6XNU3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123440035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044972566.1">
        <property type="nucleotide sequence ID" value="XM_045116631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XNU3"/>
    <dbReference type="SMR" id="A0A8I6XNU3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275610"/>
    </dbReference>
    <dbReference type="GeneID" id="123440035"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0228340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0228340.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0228340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0275610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0275610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0275610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440035"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000436">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39350 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF422">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="358"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0275610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="73943" checksum="F616A9D9468B665E" modified="2022-05-25" version="1">MRYLYFRPNTTSQARACRDHLLAHLDQCASRAHLAVLHGRLIRAHLGPDPSVAGRLVALLASPVARHDMPCARRVFDRMAQPTAFVWNCMIRGYNSCDAPADALALFRAMLRAGISPDNYTMAAVVSSSVAFAGCKLRSTGDAVHSLVRKTGFASDVFVMSGLVNFYSSFRSVDDARKVFEEMADRDVVTWTLMISAFARCCQWDDALRLLAEMQSQGTKPNKVTVISLLSACGQVQDVDRGRWVYARIHEYGIEAGLDVRNALLSMYVKCGCMSDALKAFQIMPIRNTKSWNTLIDGFVQNGKDMEALRMFEEMLSDGIVPDAITLVSLLSSCAQLGDLQKGTYFHNYIKDHGIRCDIILHNSLINMYAKCGDMAAAEMVFENMTQRDTVSWTAMLCGYVKGLQFRTAFSLFEEMKVVDVVSSEMALVSLLSACSQLGALDKGREIHTYIEEKSVTTDVWLESALVDMYAKCGCTDTAAQIFSTMQHKPTLTWNAMIGGLASNGQGKEAVGHFEQMLKLRDPNPDAITLKVVLGACAHVGMVDEGLHYFYLMLSLGIVPDIEHYGCVVDLLSRAGLLDEAYSFIKKMPIQPNPVIWGSLLAACRIHHRMELAKIIGQHIIELAPNDVGAHVLISNLHAEEGQWDDVEQVRGLMGSRGVEKSSGCSSIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XRD2</accession>
    <name>A0A8I6XRD2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspergillus nuclease S1</fullName>
            <ecNumber evidence="3">3.1.30.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123428229</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.30.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclease type I family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044968357.1">
        <property type="nucleotide sequence ID" value="XM_045112422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XRD2"/>
    <dbReference type="SMR" id="A0A8I6XRD2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207110"/>
    </dbReference>
    <dbReference type="GeneID" id="123428229"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0172140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0172140.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0172140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428229"/>
    <dbReference type="OrthoDB" id="441446at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990238">
        <property type="term" value="F:double-stranded DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043765">
        <property type="term" value="F:T/G mismatch-specific endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080187">
        <property type="term" value="P:floral organ senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd11010">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.575.10:FF:000002">
        <property type="entry name" value="Endonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="entry name" value="P1 Nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003154">
        <property type="entry name" value="S1/P1nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146:SF14">
        <property type="entry name" value="ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146">
        <property type="entry name" value="ENDONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02265">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="Phospholipase C/P1 nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269149" description="Aspergillus nuclease S1" evidence="11">
        <location>
            <begin position="26"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="314" mass="34717" checksum="83EAD4BB8F1A69D2" modified="2022-05-25" version="1">MASSGAFMVAAVLGLVLISAPAARAWSAEGHMLTCQIAQDLLEPAAAQAVKNLLPEEAGGDLSAMCVWPDQVRHWYKYRWTSPLHFIDTPDKACTFDYARDCHDPSGAKDMCVAGAVANFTSQLMHYKQGSADRKYNLTEALLFLSHFMGDIHQPMHVGFTSDMGGNSVNLRWFKHKSNLHHVWDREIILTVLAERYGKDIAAFRQDLQHNITKGSWSDESSWKDCADIMSCPTKYATESIGLACKWGYDGVHDGDTLSEEYFASRLPIVTRRIAQGGVRLAMFLNRAFGDHKAHSSGDVAAPVNLAVAHDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS31</accession>
    <name>A0A8I6XS31_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123397691</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948164.1">
        <property type="nucleotide sequence ID" value="XM_045092229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS31"/>
    <dbReference type="SMR" id="A0A8I6XS31"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0420460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0420460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0420460"/>
    </dbReference>
    <dbReference type="GeneID" id="123397691"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0420460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0420460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0420460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397691"/>
    <dbReference type="OrthoDB" id="3064467at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF983">
        <property type="entry name" value="NB-ARC DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="202"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="445"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="559"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1027"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1032"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0420460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1050" mass="116934" checksum="3D107ABDA4B0218E" modified="2022-05-25" version="1">MVLPAGLVAEKAAATAFANVVLGRLIKELENKYQIWSSIHTEISSLQEDLRILAAAVDDQQVSVAGSCSTVVRRVYGEQIRELTHDIEDCVERFLHRITCKAGASRARRTAHALRSLRRRLRFAAKIKEFRSRVVEARERALNAATGAASASVPDSGGAQSSSKQGSMQGCPPVGIEEAIKELRALLHIEPNDQDGAAIQAQLRVVAMVGFGGSGKTTLARAVYEIVHRQRALPCAWIDGHSLDHTDADSIVKHIQDMLRSGEGCPPSTTLSLHQDKGKRHLIVIDDIKKKHLIHWDTVRNAFRNNGRIILTTNNRFVANRCCNHPREEGRCTFGHVYNMRSLGEEDSRKIALLGRDTPEIVMGSEKLLKKCDGLPLALYSVACELGSEMEPTGKFCKKLCSDLGTYLEREDDEPNFARLRVVLRENYTSLSNHTVRTCLLYLGIFPIDRPLKKNVIIRRWLAEGYARHYEGRHQSVADENFKAFTHRSIVLPAVPISNFIEKACKIHGIMHAFMLHKSMCRKFIMPFGTQHKKVRHLFIHDSQCGNYEIKPDMDLSRARSLTVTGQAGGAISRFNKYKLARVLDLEGCIDVKDCHVKDICKLWNLRYLSLGPDITEIPKEIAQLKLLETLDVSKTRVNVLPVEAIGLPCLIHLIGKFKLEDPVKMKKPPKECMLETVAGIVADNGRGCLQLMDHMKKLNKVKIWCEGEETKQGQIDMNNQLFKAIQKYIKKPPMDELNDRSLSLHFQKLPQGSLTVLDQLCEDHSSRAGDSYHLSSLKLHGNSSSATLPKFVALFHDLTTLSISTSMTVTQHLLSVIGEMAKLLYLKMTANSIDGDGFVIEDGKFRSLKRLCLVLKVVRPLVLSIEDGGGLAISSLQLICKNLDGLSCVGIRHLPELKEIVLHPDVDEEARQAWEAEARNHHNMPHVFFIPSDLEDTDHSRVEAAINAFSPVEVAHSDHMRQEPAAAVVGEPAHEDHMEVELENNFKAPVKSGPVVNFHVNGVHEGWDKEALVKPPNMKKGKLAQMQKNTAANPAAQLATASACNPTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XS55</accession>
    <name>A0A8I6XS55_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XS55"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0498550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0498550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0498550"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0498550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0498550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0498550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF5">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 8-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="2">
        <location>
            <begin position="151"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="MULE transposase" evidence="3">
        <location>
            <begin position="362"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0498550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="56085" checksum="7E60FA663F939682" modified="2022-05-25" version="1">MLGDFVEDQDNSPVQLEDLDAQTTTFHDILLDSWKPTLFDDILLDQVDRNNEGIRGAFEREQNHTWNLDSPTYVDDGSSTDSREAGTSTNACCLQVPNSENENNDINEETIDEFLENERRAAEGGNDRSIDSELKPKMGMQFSTREEAQKFLNFYAFVAGFSISVVSTARTTSKKRNGEVVRVTLKCNKYGHNTEAERENLIVHRKSTVIAKTDCKAQMIVALKDGRWRITNLNLDHNHDLNPGSRFFRSHVYMSDEEKALIRTIKRCNIPTKKIVAVLAYIRGGMDKLPYNKRKVGNYGTTINRELENSDVMEVQQYFQKKQAESPGFCYSMEVDAKNKVRSVFWTDARSRTVYQEYGDCISFDTTFLTNKYNLPFAPFVGISPHGKTYLFACALIVNETTNTFKWLFQQFLAAMGGKAPKSIITDQDKAMESAIKDVFPQATHRTCLFHVMKKSEEQMCRVFQLNQGLYEDFHDIVNNSLTKKEFETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSN4</accession>
    <name>A0A8I6XSN4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSN4"/>
    <dbReference type="SMR" id="A0A8I6XSN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0215940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0215940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0215940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="4">
        <location>
            <begin position="47"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="781"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="604"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0215940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="828" mass="95535" checksum="27AE8524BE786C71" modified="2022-05-25" version="1">MAGVVRLWNDWEVEILVLVSFMLQVFLLMFAWMRRRNISVVPRTLLWLAYLLADSIAIYILGHMSFCGKSHGHQQLMAFWARFLLVHLGGQDNITAYSIEDSQLWPRHLLTFVVQTVGVAYVLYKYVAGSWALVTAAVLIFVSGVLKYGERVWALKLSSLENMGKFLDSRKLIEAKREQDAPWDQRKNLDPEAIQEQHVQWGQGENLDAEEVLQGAHDLLPICMAQFVDYKFWPSPFQSKAVELLNNKGQMFELVEMQLSLMYDFLYTKAAVVFTWYGCFIRAISSAVTITTFFLFQSCIGKNDFNRVDAIVTYILIVGAVFLEMTALLKAMGSTWTCALLHARRCHWLHSVVIYVRRRVKAAERNRRWSGSIGQPELLNLPHGSGGCMATLLCKDLWKKLNHHTLPVISDGTKKLVLDEVRRMVEACDGKEDIMRSYNGQCALNPWSGLFKDPTSHAGINFDERILSWYFATKIFFSWSSCVEQHGHVEAITAVSNYMTFLVAVHPYMLPSPVRPGLYASTEAAYVGLDFSDRDFISKQLGLDRRRRMWELDERTDVDELFRVRGKLAIISEDLDIIRKDLTTRRWSEADRRSTGIVFMGNAREEMWKTVKELDRRLEELNRRMEELDDLTLEVGGRREKLEVLNRRRKELDQRLEELNRRREELDDVTLEVGGTRDVLEVLNRRRKELEVLNRRRKELDQRLEELADLTLEVGGTREKLEVLNRRRKELDRRLKELDDLTLKVGSRREKLKVLNRRKNELDGIRLTELDRRSEVDSIMEVERGPGPGPGGAAQKNEQAGGARQKKRGAAFWTSTSTCLGPWSGDCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XU84</accession>
    <name>A0A8I6XU84_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123440817</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973300.1">
        <property type="nucleotide sequence ID" value="XM_045117365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XU84"/>
    <dbReference type="SMR" id="A0A8I6XU84"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314760.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314760"/>
    </dbReference>
    <dbReference type="GeneID" id="123440817"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0262970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0262970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0262970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0314760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0314760.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0314760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440817"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="5">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0314760.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="104" mass="11224" checksum="B79B2A02ED979D0C" modified="2022-05-25" version="1">MADRVTKLASQRAVVIFGASNCFMCHAVKTLFTELGVSWTVHELDKDPRGKDVERALVGMVGRNPPVPAVFIGGRLVGTTDQVMTLHVGGQLVPLLRQAGALWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XVY0</accession>
    <name>A0A8I6XVY0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17 18">Plasma membrane ATPase</fullName>
            <ecNumber evidence="3 18">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123450140</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="3 18"/>
    <dbReference type="RefSeq" id="XP_044983476.1">
        <property type="nucleotide sequence ID" value="XM_045127541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XVY0"/>
    <dbReference type="SMR" id="A0A8I6XVY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0419450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0419450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0419450"/>
    </dbReference>
    <dbReference type="GeneID" id="123450140"/>
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        <property type="component" value="Chromosome 4H"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:proton export across plasma membrane"/>
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        <property type="term" value="P:regulation of intracellular pH"/>
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        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
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        <property type="entry name" value="Plasma membrane ATPase"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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        <property type="entry name" value="ATPase_P-typ_P_site"/>
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        <property type="entry name" value="P-type_ATPase_IIIA"/>
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        <property type="entry name" value="P_typ_ATPase"/>
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        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
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            <end position="101"/>
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        <location>
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            <begin position="32"/>
            <end position="104"/>
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            <begin position="1"/>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYN4</accession>
    <name>A0A8I6XYN4_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123401899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044951692.1">
        <property type="nucleotide sequence ID" value="XM_045095757.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551990"/>
    </dbReference>
    <dbReference type="GeneID" id="123401899"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.6HG0458660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0551990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0551990.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0551990"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401899"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="entry name" value="DUF7874"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37754">
        <property type="entry name" value="CALCIUM ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR37754:SF1">
        <property type="entry name" value="CALCIUM ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25284">
        <property type="entry name" value="DUF7874"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0551990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="172" mass="19322" checksum="EEA307CB4BB37076" modified="2022-05-25" version="1">MGLPFSALNKLGVPGLGAVTTGQVYDRHFKNKDTRTFEEFHLAYVEFCKYFNTVLPGQDFDTPDLEVIRKWYADTWEPLQKDDEKKKMFIEYMQEKVTEATVEDNLFIMAGLAAPAAAIIAKKSGESIPQVKKFKVHLIPNVVFVPLCTLVAIMGATAVQMSKKSKDNKPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y101</accession>
    <name>A0A8I6Y101_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123397057</name>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">subsp. vulgare</strain>
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    </evidence>
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    <sequence length="201" mass="21378" checksum="FC5A2BD63B373A8C" modified="2022-05-25" version="1">MANAKLLAVFALLQVLSVLLHVHGVSGAISLTHKRAKRPGGGKCHISGFLRGKAGKCNPEHGSDCCVSGHRYPQFRCSPPVSAETPAILTLNSFAAGGDGGGKSFCDNRFHPDSARVVALSTGWLRLDGTRRCNKMIRINGNGRSVLAKVVDECDSVNGCDEEHNFEPPCPNNAVDGSPAVWKALGLKQSIGEFKVTWSDV</sequence>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1W1"/>
    <dbReference type="SMR" id="A0A8I6Y1W1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0317200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0317200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0317200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF445">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="26"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0317200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="519" mass="56388" checksum="C45E2ABFE74CDF75" modified="2022-05-25" version="1">MAGGAFVDTAGGKQYPGKLTLYVVFTCVVASTGGLIFGYDIGISGGVTSMDPFLLKFFPEVYRQKQAASKSNQYCQYDNQLLQAFTSSLYLAALVASFFASTVTRVLGRKWSMLAGGFTFLVGAALNGAAQNVAMLIVGRMLLGVGIGFANQSVPVYLSEMAPAHLRGMLNIGFQLMITIGILAAELINYGTNKIKGGHGWRVSLALAAVPAVIITVGALFLPDTPNSLMERGHPEASRRMLRRIRGTDDIGEEYADLVAAREISKQVQHPWRNILMRKYRAQLTMAVLIPFFQQVSGINVINFYAPVLFETLGFRGDASLLSSVMTGGVLVLGSVVSMFTVDRLGRRKLFLQGGAQMLVSQIAVGTLIAVRFGTSGVGEMPRGYAAAVVLFICVYVAGFVWSWGALGWLVPSEIFPLEIRSAGQSITVSVNMFFTFVIAQAFLTMLCHMKFGLFYFFAGWVVVMTTFVAFFLPETKNVPMEEMVLVWKGHWFWSRFIGDADDIHAGGKSGAAVDVITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y5H4</accession>
    <name>A0A8I6Y5H4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Legumain prodomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123401780</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044951566.1">
        <property type="nucleotide sequence ID" value="XM_045095631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y5H4"/>
    <dbReference type="SMR" id="A0A8I6Y5H4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0599070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0599070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0599070"/>
    </dbReference>
    <dbReference type="GeneID" id="123401780"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0496570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0496570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0496570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0599070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0599070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0599070"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401780"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21115">
        <property type="entry name" value="legumain_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.130:FF:000001">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000005">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043577">
        <property type="entry name" value="AE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048501">
        <property type="entry name" value="Legum_prodom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046427">
        <property type="entry name" value="Legumain_prodom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000">
        <property type="entry name" value="HEMOGLOBINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000:SF42">
        <property type="entry name" value="LEGUMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20985">
        <property type="entry name" value="Legum_prodom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500139">
        <property type="entry name" value="AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158842" description="Legumain prodomain domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Legumain prodomain" evidence="11">
        <location>
            <begin position="380"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0599070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="493" mass="54793" checksum="0A8A0470703FD3AD" modified="2022-05-25" version="1">MAPWWCFGLLLLLCALAGADASKGRWDPVIRIPREEEPATARGDGGSQEEEEDGVGTRWAVLVAGSSGYGNYRHQADICHAYQILRKGGVKEENIVVFMYDDIAKNALNPRPGVIINHPEGEDVYAGVPKDYTGEAVTAKNFYAVLLGNKTAVTGGSKKVIDSKSNDHIFIYYSDHGGPGVLGMPNLPYLYAADFIKVLQEKHASNTYAKMVIYVEACESGSIFEGLMPADLNIYVTTASNAEESSWGTYCPGMEPSPPSEYITCLGDLYSISWMEDSETNNLKEETIKKQYEVVKKRTSDMNSYSAGSHVMEYGDKTFKDEKLYLYQGFNPANTNITNKLFWQARKAAINQRDADLLFLWRRYELLHEKSKEKVNVLREISETVTHRKHLDSSIDFIGKLLFGFENGPSMLETVRPSGIPLVDDWDCLKRMVRIFESHCGSLTQYGMKHMRAFANICNNGISGTSMKEASISTCGGGHNSARLSTLIQGYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y748</accession>
    <name>A0A8I6Y748_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123404270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044954126.1">
        <property type="nucleotide sequence ID" value="XM_045098191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y748"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0621460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0621460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0621460"/>
    </dbReference>
    <dbReference type="GeneID" id="123404270"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0515540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0515540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0515540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0621460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0621460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0621460"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404270"/>
    <dbReference type="OrthoDB" id="650889at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34710">
        <property type="entry name" value="OS03G0834100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34710:SF13">
        <property type="entry name" value="OS05G0547600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3615" evidence="1">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0621460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="167" mass="18860" checksum="4EC1FFB8445624B8" modified="2022-05-25" version="1">MEAQVLARFPDRKPCQQAKDFAELALAHYNETNKSEFELATTLLSNCFSECSGDIYGHVNFTAVPQKQTAAETASKTKRLFFAELMHTPSLEAYSRAQPMRVLCVCTIDDDSCYGGCHEIFRKIANKRSDDMDYKRCHACSDRIKHPNGQLFDGGHNSSRMPYYSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAD5</accession>
    <name>A0A8I6YAD5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123411555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044960456.1">
        <property type="nucleotide sequence ID" value="XM_045104521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAD5"/>
    <dbReference type="SMR" id="A0A8I6YAD5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0663670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0663670"/>
    </dbReference>
    <dbReference type="GeneID" id="123411555"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0550560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0550560.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0550560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0663670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0663670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0663670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411555"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF68">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0663670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="503" mass="54470" checksum="2A1B3EBC19204C1A" modified="2022-05-25" version="1">MGSQGVPNATMAGHDDVGGAAKAHFVFVPLMYQGHVIPTVDTALLLATHGALASVVATPSNAARIRPTVDFARRSGLPIRLVELPLDCAAEGLPEDADDVDKIPFGLAPNYFRALTLLAGPLERHLRAHPPYPTCIVSDFCHAWTVQVAANLKVPRLSFFSMCAFCTLCQHNVERYNAYDGVADDNEPVVVPGVERRIEVTRAQAPGFFRAPGFEKLADEIELALAESDGVVMNSFVEMEPEYVAGYADARKMKVWTIGPVSLYHQHAATLAKRGNTTTAIDADECLRWLEGKEPNTVVYVSFGSIVHADPKQVVELGKGLEASGHPFVWVLKNPDQYGEDVRGFLRDLEERVAGRGMLIRGWSPQVLILNHAAVGGFVTHCGWNSTVEAIAAGLPVVTWPHFSDQFLNEKLAVEVLGIGVSVGIKEPLMWVAMKEIVVGRDVVEAAVRSIMDGGEEGEERRRKAVDLSEKARAAVQEGGSSLGNLLDLIKSFEMDVGGCTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YH98</accession>
    <name>A0A8I6YH98_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123448854</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981817.1">
        <property type="nucleotide sequence ID" value="XM_045125882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YH98"/>
    <dbReference type="SMR" id="A0A8I6YH98"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383320"/>
    </dbReference>
    <dbReference type="GeneID" id="123448854"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0318770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0318770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0318770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0383320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383320"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448854"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF150">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">subsp. vulgare</strain>
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        <subcellularLocation>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0513490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="311" mass="34110" checksum="6A39BBB1E9C16110" modified="2022-05-25" version="1">MGRAPCCDKASVKKGPWSPEEDATLKSYIEQNGTGGNWIALPQKIGLRRCGKSCRLRWLNYLRPNIRHGGFSDEEDRIILSLYISIGSRWSIIAAQLPGRTDNDIKNYWNTRLKKKLFGKQSRKDQRQHQFGRQGANGGHKEEASEGSAGNNGVAAGAYNWHQHTMAMPPHPMPGSMVEGNLIGEGVDESIRKLLFKLGGSSFAALPAPPCLPPPMYGEAPNFVAPSVHTAPLNEGGIHCSSVLPALELDESFQFNQVKLDGLECFFGMGDQSMRWNEVSPLVCPNTTVASSSQGMQQYCLLEEPVDLGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8I6YVI9_HORVV</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVI9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0688940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0688940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0688940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0572060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0572060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0572060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0688940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0688940.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0688940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0688940.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="130" mass="15336" checksum="71BEF2239CD88A3E" modified="2022-05-25" version="1">MTVLKFPKWAINAINSQMPHFLWGNIGDQHKYHLAHWGLVSRKKEFGRLGIPNIREYNMALLASWGKRFYNSSNSDWKKLLAYKYNVDSPSIFWSRQQGGSSFWKGISWAFQAARKFYQWKLGDGNNIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YYY0</accession>
    <name>A0A8I6YYY0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Hordeinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YYY0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0730450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0730450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0730450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0730450.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="108" mass="12044" checksum="25BAC1B5461127BF" modified="2022-05-25" version="1">MKEVVMERRPLHKEFHVRVVDKVRHRRQHEGESPANCVTGDEHEGKSPASCGTGDKRATTSVPRRGACVEGIEGVALIEANDGDGNWRDKMAMAAVSVLHQRHQRPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z0P1</accession>
    <name>A0A8I6Z0P1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0P1"/>
    <dbReference type="SMR" id="A0A8I6Z0P1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0474030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0474030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0474030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0571800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0571800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0571800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="521"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0571800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="66530" checksum="6274751C03175A1D" modified="2022-05-25" version="1">MFWLLRIQAPLHYHPMNAGPRKWRICACGNIHQGTSSLRDAEANGHRHGERKGKRKHEGAYIDKDGEAQTFDRKKISRKRGGAMRGRGWKYGSGFVDGVFPVLSPMAQDILELVQKGTDVAKVWESLDNVPPTHALWDDVLNVAVQLRLNRQWDQVISVCEWIVHRSSFRPDVVCYNLLIDAYGRRRLLGKAEAMYAALLEARCVPTEDTYALLLRAYCTAGSLHRAEGVISEMREHGIPPSATVYNAYLDGLLKARCAEKAVEVYQRMKRERCRTNTETYTLMINVYGKSKQPMSAMKVFKEMQSIGCKANICTYTALVNAFAREGLCEKAEEVFEEMQQAGHEPDVYAYNALMEAYSRAGFPQAASEIFSLMQHMGCEPDRASYNILVDAYGRAGLHREAEAVFESLKRQGMAPTMKSHMLLLAAHARSGNVARCEEVMAQLHKSGLAPDTIALNAMLNAYGRAGRLDDMERLLAAMERRGTRDVGTYNVAVNAYGRAGYLERMEAAFASLERRGLAADVVTWTSRMGAYARKKEYRRCLEIFEEMVDAGCYPDAGTAKVLVAACSDERQVEQVTAIVRSMHKEAKTLFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZC83</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123410455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959337.1">
        <property type="nucleotide sequence ID" value="XM_045103402.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959338.1">
        <property type="nucleotide sequence ID" value="XM_045103403.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959339.1">
        <property type="nucleotide sequence ID" value="XM_045103404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZC83"/>
    <dbReference type="SMR" id="A0A8I6ZC83"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717330"/>
    </dbReference>
    <dbReference type="GeneID" id="123410455"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0595040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0595040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0595040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717330"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410455"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="478"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="513"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="548"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="583"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="618"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="653"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717330.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="698" mass="76467" checksum="F869B81C35ACBA3E" modified="2022-05-25" version="1">MLPLPLRRHFLFLYSRRNPSFIPFSIAATAISTLPTDSAAAAAAAATPISDRLRLLHSLQAVPANLLLSHPLPSSAHLCIAAHLLARARLFPHSRSLLSRLLAPGHRPHLAASLVDLLHRAALALGPRRSALPSVVDTLLSLLADRGLLDDAVLALGRVRELRVPPNTRTCNHILLRLARERRGVLVRRLFEQVPAPNVFTFNIVIDFLCKEGELTEARALFSSMKAIGCPPDVVTYNSLIDGCGKCGELEEVEQLVGEMRRCGCTPDVVTYNALVNCFCKSGMMERAYSYFAEMKREGVVANIRTYSTFVDAFCKEGMVREAMKLFAQMRIRGMAPNEVTYTCLVDGTFKAGRLDDAFVLIDEMVQQGVPLNVVTYTVQVDGLCKEGKIAEAEDVFRLMEKAGVKANELLYTTLIHGHFVNKNSERALDLLNEMKDKGMELDVSLYGALICGLCNLRKVDEAKSLLNKMDECGLKPNSIIYTNIMDACFKEGKGSEAIALLHKMQDSGFQPNVVTYCALVDGLCKAGSVDEAVSHFNKMSDLGLEPNIHTYTALIDGLCKSGCLTKAVGLLDEMVGKGLSLDKVVYTSLMDGYLKQGNLQDAFALKADMINSGLQLDLYGYTCFLWGFCNLNMMQEAREVLSEMIGNGITPDAVIYNCLISKYMKLGNIEEAASLQNEMESVLSSFTNGDTALGSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6ZDQ5</accession>
    <name>A0A8I6ZDQ5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6ZDQ5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0724920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0724920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0724920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0724920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0724920.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0724920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF78">
        <property type="entry name" value="OS06G0232700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="63"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0724920.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="45698" checksum="18D598FA3563AB84" modified="2022-05-25" version="1">MAEMPQLLNGYHDNHHRCQLFINPNHENPPQTFRLRTVKTPWSIENRFLEHLEAYGLLHFANIAARRGSFTADPSLLTSLVDRWRPETHTFHFRCGDLAPTLKDVSMITALPIRGVRVVHPRVSPNWPADVSARLRLEMPISDCSGSPRGVPHSWLRINFEILSTHADPETTRRHLFAYLLWLFGVMFPNSHGDVVLPGLIYFAAKIVDEPLPQNPPYSFGSALLSHTYRGLCDATQKTAFTSKAPLLCVSYEFLQLWSWEYLPVGRPQIVEPATPYNYGEGVVTMSTRWMLGRKKWSTKIAKNCYPIYHDQFELLNDSLITWNPWTEAHIDMVFGSQHMPVECVRDSAFWMTRCNLLYLWFVEPYNPDHVMRQFGLYQDIPPPVPRCIDEETHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B6M9</accession>
    <name>A0A8I7B6M9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123450964</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay. Functions as a positive regulator of the cytokinin signaling pathway. May play a regulatory role in salt and drought tolerance during plant development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044983911.1">
        <property type="nucleotide sequence ID" value="XM_045127976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B6M9"/>
    <dbReference type="SMR" id="A0A8I7B6M9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332600"/>
    </dbReference>
    <dbReference type="GeneID" id="123450964"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0277150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0277150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0277150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0332600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0332600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0332600"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123450964"/>
    <dbReference type="OrthoDB" id="1673781at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080038">
        <property type="term" value="P:positive regulation of cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.160:FF:000001">
        <property type="entry name" value="Histidine-containing phosphotransfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF56">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="9">
        <location>
            <begin position="35"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="7">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0332600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="143" mass="15877" checksum="6E67E71819863B29" modified="2022-05-25" version="1">MAAAALRAQLNALIGSMFATGIVDENFQYLQSVEQEGFLAEILNLFFHDADKILNEVVLLLNQPVVNFGRVGALVHQLKGCSSSVGAKKVTLSCIHFRQFYEAESKEGCLMALALVRNEFYDVRNKLQTMMQLEQQIAAMGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B787</accession>
    <name>A0A8I7B787_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123430507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IAA-amido conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970309.1">
        <property type="nucleotide sequence ID" value="XM_045114374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B787"/>
    <dbReference type="SMR" id="A0A8I7B787"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180880"/>
    </dbReference>
    <dbReference type="GeneID" id="123430507"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0149540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0149540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0149540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0180880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0180880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0180880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430507"/>
    <dbReference type="OrthoDB" id="655583at2759"/>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GH3_C"/>
    </dbReference>
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        <property type="entry name" value="GH3_M"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03321">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="328"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
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            <end position="532"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008068"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0180880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="563" mass="62218" checksum="6C2CFB343C890500" modified="2022-05-25" version="1">MTSDVDSVQERVLAEILARNAGSEYLQSCGLDDGLVDRAIFRAKVPVVSYDALRPYIQRIANGDISSIMSTHPVSDFLTSSATSGGEPKLIPNVEDEGGRRQLIFGLRAAVANLHSPGIDKGKGLYFLFVKPETKTPGGLTAWSVQTSIYKNENFKNFSRNHTSPRAALLCADTFQSMYAHTLCGLCQRHDVLRVGAVFASTLLRAIRFLQLNWEQLAADIEAGGLTSRVNDASVREAVAGILHRPDPELAQFVRESCCKDEWAGIVTRIWPNTRYLDAVVTGAMAQYISALNYYSGGLPIVCSMYGASECHFGINLRPLCDPADVSYTIMPNMAYFEFLPVHEEATGGGQPVELARVEAGREYEVVVTTYAGLNRYRVGDVLRVAGFHNAAPQFQFVRRNNVLLSVETDKTDEVELQCAVGRASELLRLHGASVAEYTSRACTRRIPGHYVIYWELLAAGDAAVDKETLDECCLEMEEALNAVYRRSRVVDGSIGPLEIRVVRPGTFEELMNYAVSRGTSIGQYKVPRCVTLPSIIHLLDSRIMSTHFSPTLPHWTATRRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7A5</accession>
    <name>A0A8I7B7A5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Wound-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979087.1">
        <property type="nucleotide sequence ID" value="XM_045123152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B7A5"/>
    <dbReference type="SMR" id="A0A8I7B7A5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383630"/>
    </dbReference>
    <dbReference type="GeneID" id="123446576"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0319040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0319040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0319040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0383630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0383630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0383630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446576"/>
    <dbReference type="OMA" id="APNIEWW"/>
    <dbReference type="OrthoDB" id="667779at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009798">
        <property type="entry name" value="Wun1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33703:SF13">
        <property type="entry name" value="OS03G0299600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33703">
        <property type="entry name" value="OS07G0691300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07107">
        <property type="entry name" value="WI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0383630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="14577" checksum="04A6C2A593BD72D5" modified="2022-05-25" version="1">MMRLLTGAGTNDEFRFCPRSVDAFGSTVVAEGSADGAGQLLYWVHAWTVGPDGVITQLREYFNTDLTVTRLSAAAATKNASASSPTHSSSSSTSSSPSSSTSSGLSSPAHTPKWPKCLWQSRRTDRARKSLPGLVLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7C8</accession>
    <name>A0A8I7B7C8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:26210"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19626"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="9"/>
    <dbReference type="RefSeq" id="XP_044983203.1">
        <property type="nucleotide sequence ID" value="XM_045127268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BDD2"/>
    <dbReference type="SMR" id="A0A8I7BDD2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413370"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413370.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413370.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413370"/>
    </dbReference>
    <dbReference type="GeneID" id="123449904"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413370.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413370.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413370"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449904"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000041">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000063">
        <property type="entry name" value="probable low-specificity L-threonine aldolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007825">
        <property type="entry name" value="PRK10534.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF11">
        <property type="entry name" value="AROMATIC AMINO ACID BETA-ELIMINATING LYASE_THREONINE ALDOLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="11">
        <location>
            <begin position="7"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="369" mass="39039" checksum="1EC9A8805217472D" modified="2022-05-25" version="1">MAAKVVDLRSDTVTKPSEAMRAAMAAADVDDDVLGADPTARRFEAEMARIMGKEAALFVPSGTMANLISVLAHCDARGSEVILGDDSHIHVYEHGGISTLGGVHPRTVPNNPDGTMDVDRIVAAIRSPDGALYYPTTRLICLENTHGNSGGKCLTVEYTDKVGEVAKAHGLKLHIDGARIFNASVALGVPVDTLVRAADSVSVCLSKGIGAPVGSVIVGSKAFINKAKILRKTLGGGMRQVGVLCAAAEVGVRDTIGKLTDDHRKAKVLADGLKKIEQFTVDLTSVETNMVFFHMADPRITPAKLCKVLEQRNVLAMPTSSKSVRLVVHYQISDSDVQYTLTCIEEAVGEILACDAKLERPTNGAAANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BES3</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0709800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="160" mass="16695" checksum="0D705A52D009E4B9" modified="2022-05-25" version="1">MHVRLHDATRLLLPPLPSAMASSAAARHIPLLVMVLLAVVLLLSSSSGLAAAEAPRGSARAAAVAAGRARQDFHVATTPLHDASVAENGAGRRPVGGRRRRRRVDRGRERERGGGTGAWAFSGMLPRGFVPPSGSSACHNDMPATATDAQFYTCGSSVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BEU1</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BEU1"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0711880.1.CDS1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0711880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0711880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0711880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0711880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25933" checksum="1E3EFFF99A3D61F5" modified="2022-05-25" version="1">MSMCGYRWYPEYIVEEQYRDFNQSQYICTVRVFLDYPGAEEPIHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFSYVPASQPGEEGYLSGVYFDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTTLMQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BGK9</accession>
    <name>A0A8I7BGK9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Fiebig A."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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                <person name="Muehlbauer G.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    <reference evidence="2" key="1">
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            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
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    </dbReference>
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    </evidence>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">cv. Morex</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
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    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
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            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
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        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_045103878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CRP9"/>
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    <dbReference type="STRING" id="112509.F2CRP9"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="UniProtKB-KW"/>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ85520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0692390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="96" mass="10792" checksum="7BB3B75E8F69084A" modified="2011-05-31" version="1">MVCTKCEKKLGKVIVPDKWKEGASNTFEGGGRKINENKLLSKKSRWTPYGNTKCIICKQQVHQDGKYCHTCAYSKGVCAMCGKQVLDTKLYKQSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F2CT42</accession>
    <name>F2CT42_HORVV</name>
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        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123402900</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
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        <property type="protein sequence ID" value="BAJ86013.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="SMR" id="F2CT42"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573290.1">
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_A20"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF112">
        <property type="entry name" value="ZINC FINGER A20 AND AN1 DOMAIN-CONTAINING STRESS-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
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        <location>
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        <location>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573290.1.CDS1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F2CYT0</accession>
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        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123430024</name>
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    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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            <taxon>BOP clade</taxon>
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            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Shoot</tissue>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK356786">
        <property type="protein sequence ID" value="BAJ88001.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="BAJ97964.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044969892.1">
        <property type="nucleotide sequence ID" value="XM_045113957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CYT0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0039670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0039670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0039670"/>
    </dbReference>
    <dbReference type="GeneID" id="123430024"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0030770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0030770.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0030770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0039670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0039670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0039670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430024"/>
    <dbReference type="HOGENOM" id="CLU_025169_0_0_1"/>
    <dbReference type="OrthoDB" id="1882547at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055502">
        <property type="entry name" value="DUF7074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055503">
        <property type="entry name" value="DUF7075"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31469:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31469">
        <property type="entry name" value="OS07G0633600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23269">
        <property type="entry name" value="DUF7074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23272">
        <property type="entry name" value="DUF7075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7074" evidence="3">
        <location>
            <begin position="154"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7075" evidence="4">
        <location>
            <begin position="314"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAJ88001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAJ97964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0039670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="624" mass="70197" checksum="80F7316B40797336" modified="2011-05-31" version="1">MLGRGVGMSAPLWRGGALDLRAALRSGGNLLFGLFVAAVLAFTLLAAVHSPDDPLLHPSSHQLTAFLTSATSNSTFLADDSVLRTGEDFATGSNSSEDAPTTAGPKFIELSDVGSEKTEAETEQSVTVDTDADSNSAAQEEKPIVEAVSCDTEAPVDCTGDRELFNLLMRTAIERFPDLHFYRFGRPVVVPESPMACDLAWRFRPAEDARGRTTYYKDYRRFTLARDVNTCSLVVGSVGEYHSGTGAKRSGRRKGKKGKKGKREAPVTTDFVPAKTQMRIDENGANADTTTAADQVFVVGDAVNDSMPVVASESDFSRGRYLIYMGGGERCKSMNHYIWGFLCALGEAQFLNRTLVMDFSVCLNSRYTASGKDEEKDFRFYFDFEHLKESASVIDQSQFWTDWGKWHKKDRLKNHYTEDIKVTPMKLRDVKDTLIMRKFGHVEPDNYWSRVCEGETEAMIKRPWHFLWKSRRLMEIVSAIASRMSWDFDSMHVVRGEKAQNTQLWPNLDADTSPENLLTTLNDKVGAGRYLYIATDESDKSFFDPLKNKYKTRFLDDFKDLWDENSDWYAETKELNNGKPVEFDGYMRVAVDTEVFLRGKRKLETFNDLTRDCKDGVNTCAASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2Q1</accession>
    <name>F2D2Q1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123424554</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
            <tissue evidence="11">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="AK358158">
        <property type="protein sequence ID" value="BAJ89372.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362762">
        <property type="protein sequence ID" value="BAJ93966.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044964120.1">
        <property type="nucleotide sequence ID" value="XM_045108185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2Q1"/>
    <dbReference type="SMR" id="F2D2Q1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106380"/>
    </dbReference>
    <dbReference type="GeneID" id="123424554"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0087210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0087210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0087210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0106380.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0106380.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0106380"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123424554"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="132"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ89372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ93966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0106380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="316" mass="35500" checksum="0230DC7D24D08F91" modified="2011-05-31" version="1">MHRSAGVAMAWNVFRFCTALRGLGSIMILLVLAIVGVTYYAVVLCNYGPALLLGGGATLAALAVLLLFHFLLAMLLWSYFSVVFTDPGSVPPNWNLDFDEERGETAPLSSSDFNSQMNPQQSMALGDTGNPRMRYCRKCNQLKPPRCHHCSVCGRCILKMDHHCVWVVNCVGALNYKYFLLFLFYTFLETTLVTLSLLPHFIAFFSDVEIPGTPSALATTFLTFVLNLAFSLSVLGFMIMHISLVSGNTTTIEAYEKKTSPRWMYDLGRKKNFAQVFGNDKKYWFIPAYSEEDLRRMPALQGLDYPVRTDLDGQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2W7</accession>
    <name>F2D2W7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123427304</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK358224">
        <property type="protein sequence ID" value="BAJ89438.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967246.1">
        <property type="nucleotide sequence ID" value="XM_045111311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2W7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183450"/>
    </dbReference>
    <dbReference type="GeneID" id="123427304"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0151770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0151770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0151770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0183450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427304"/>
    <dbReference type="OrthoDB" id="1716611at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055241">
        <property type="entry name" value="Armadillo_rpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36793">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36793:SF1">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22915">
        <property type="entry name" value="ARMH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Armadillo-like repeats" evidence="1">
        <location>
            <begin position="170"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAJ89438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="319" mass="35753" checksum="C81740F8C9A2A578" modified="2011-05-31" version="1">MATTTLFHPLSIPVAGGRVRRCPLTLPAPTRATPRRSTPLLVARAKRTNNSSAAPKREADEEVEVEEELPWIQDKALDLVEFTGTVTQAIPGPRVGSSPVPWLLAVPLAYVGISFALSVVRTVRRFTSPRTQKKKRVTKNIFLLKSLDELFQKGREAVDFPAIQELMQKTGFDMDDVVRKYIRYTLNEKQFNPDVVVDLIHLRKASMLEDNEVAEILNEISRRIVREKGPIVMDLSGFTEQGFKRKLAVQTLFGKIMYLSELPEFCSRDGSLVVKEIFGVTDEDADSLRSRTLSEAGDIESLEKMVDDSDFEHGTTSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2D2Y6</accession>
    <name>F2D2Y6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphoribosylglycinamide formyltransferase, chloroplastic</fullName>
            <ecNumber evidence="3">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">GAR transformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC123410286</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="3"/>
    <dbReference type="EMBL" id="AK358243">
        <property type="protein sequence ID" value="BAJ89457.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959128.1">
        <property type="nucleotide sequence ID" value="XM_045103193.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044959129.1">
        <property type="nucleotide sequence ID" value="XM_045103194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D2Y6"/>
    <dbReference type="SMR" id="F2D2Y6"/>
    <dbReference type="STRING" id="112509.F2D2Y6"/>
    <dbReference type="PaxDb" id="4513-MLOC_18677.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678560"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678560.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678560.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678560"/>
    </dbReference>
    <dbReference type="GeneID" id="123410286"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0563210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0563210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0563210"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0678560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0678560.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0678560.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0678560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410286"/>
    <dbReference type="eggNOG" id="KOG3076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_4_0_1"/>
    <dbReference type="OMA" id="MYGRAVH"/>
    <dbReference type="OrthoDB" id="2018833at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000011">
        <property type="entry name" value="phosphoribosylglycinamide formyltransferase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="15">
        <location>
            <begin position="79"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ89457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0678560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="292" mass="30637" checksum="F2A1017D46846181" modified="2011-05-31" version="1">MEAAAVASSGSGLRCSLNPAPSQHRRGARVGLAAKRQQCCAVACGRFLLRPNARVYTAASASASAVMASGAGDSGVRRKRLAVFVSGGGSNFRSIHGAALGGKVNGDVVALVTDKPGCGGAEYARCNGIPVVVFPKSKSAPEGVSTDELLNALRDLKVDFILLAGYLKLIPGELVQAFPRSMLNIHPSLLPAFGGKGYYGLKVHKAVIASGARYSGPTVHFVDEQFDTGKTLAQRVVPVLANDTPEQLAARVLHEENQVYVEAVAALCEDRIVWRDDGVPLVRSQANPNAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2D5K0</accession>
    <name>F2D5K0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123442604</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK359160">
        <property type="protein sequence ID" value="BAJ90371.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044974630.1">
        <property type="nucleotide sequence ID" value="XM_045118695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D5K0"/>
    <dbReference type="SMR" id="F2D5K0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227860"/>
    </dbReference>
    <dbReference type="GeneID" id="123442604"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0188960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0188960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0188960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0227860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227860"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442604"/>
    <dbReference type="OrthoDB" id="600961at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090611">
        <property type="term" value="P:ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000007">
        <property type="entry name" value="Suppressor of K+ transport growth defect 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF159">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="10">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="167"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAJ90371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="438" mass="48319" checksum="7DD0D64C905A3683" modified="2011-05-31" version="1">MYSNFKEQAIEYVKQAVQEDNAGNYVKAFPLYMNALEYFKTHLKYEKNPKIKEAITAKFTEYLRRAEEIRAVLDEGGGGPPGAPNGGDAAVATRPKTKGKDAGGDGAGGDDSEQSKLRAGLNSAIITEKPNIKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVANLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKARQHMFKVHLGDTPHSLSESDFEVLGRRTEGFSGSDVAVCVKDVLFEPVRKTQDAMYFFKTDGDMWMPCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDLEVHEKFTKEFGEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2D6W9</accession>
    <name>F2D6W9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123444069</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot</tissue>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK359631">
        <property type="protein sequence ID" value="BAJ90840.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK370035">
        <property type="protein sequence ID" value="BAK01236.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976610.1">
        <property type="nucleotide sequence ID" value="XM_045120675.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044976611.1">
        <property type="nucleotide sequence ID" value="XM_045120676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D6W9"/>
    <dbReference type="SMR" id="F2D6W9"/>
    <dbReference type="PaxDb" id="4513-MLOC_60429.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279110"/>
    </dbReference>
    <dbReference type="GeneID" id="123444069"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0231300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0231300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0231300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279110"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444069"/>
    <dbReference type="eggNOG" id="ENOG502QR2Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2020306at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2D6W9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000001">
        <property type="entry name" value="NAC domain-containing protein 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044799">
        <property type="entry name" value="SOG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31079:SF20">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31079">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="74"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ90840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK01236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="345" mass="37331" checksum="21B26478165CE67D" modified="2011-05-31" version="1">MTWCNSFNDVRAVENNLASAAAVAAAAAKKQQHHHQQQQASSHASLVRTCPSCGHHAQYEQLQAAATIQDLPGLPAGVKFDPTDQELLEHLEGKARPDTRKLHPLIDEFIPAIEGENGICYTHPERLPGVGKDGLIRHFFHRPSKAYTTGTRKRRKVHTDEQGGETRWHKTGKTRPVFTDGKLKGYKKILVLYTNYGKQRKPEKTNWVMHQYHLGSDEEEKDGELVVSKVFYQTQPRQCGGSTAAAVRDAAIAAGNIIKGGGAGAGATTDHHRHHHEDGHNNNMLKEASGMVDFYSPAAAALIGYSSQAAPPNNRAVAAAAAAAAASAHLMPNFEVHTGGTGFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2D9W9</accession>
    <name>F2D9W9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123445141</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Shoot</tissue>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RING-type zinc finger family. NIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK360682">
        <property type="protein sequence ID" value="BAJ91890.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK366156">
        <property type="protein sequence ID" value="BAJ97359.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044978018.1">
        <property type="nucleotide sequence ID" value="XM_045122083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2D9W9"/>
    <dbReference type="SMR" id="F2D9W9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307740"/>
    </dbReference>
    <dbReference type="GeneID" id="123445141"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0257110.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0257110.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0257110"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307740"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445141"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000505">
        <property type="entry name" value="NEP1-interacting protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151">
        <property type="entry name" value="NEP1-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151:SF16">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="196"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAJ91890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ97359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="26386" checksum="7B2AB5F9A450B922" modified="2011-05-31" version="1">MDRRAAPPPPPPSLSGASSSPRCRFGAAWRALGRGGATVAAALGRALGALLTFAFAVVGSLVGIFIGAFMGMSTESGMLRGAGVGAVSGAVFSIEAVESCIEIWRSTESGKYSFLFVLDIISSLFSGRIVWEKVSPALQRAVQSQMSLMSTPFIDNNDLFETGCTGGMSAALINKIPAIRFSAATDSAQETDRTCCSVCLQDFGPRQFVRALPQCQHIFHVRCIDDWLQRNSSCPLCRGGVHIDHMCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DC78</accession>
    <name>F2DC78_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123408918</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot</tissue>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AK361494">
        <property type="protein sequence ID" value="BAJ92699.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK372295">
        <property type="protein sequence ID" value="BAK03493.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044957877.1">
        <property type="nucleotide sequence ID" value="XM_045101942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DC78"/>
    <dbReference type="SMR" id="F2DC78"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677850"/>
    </dbReference>
    <dbReference type="GeneID" id="123408918"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0677850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0677850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0677850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408918"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000006">
        <property type="entry name" value="Nuclear transcription factor Y subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF106">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT C-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="87"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ92699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK03493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0677850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="199" mass="21573" checksum="75F912AF3C95AD4F" modified="2011-05-31" version="1">MEPKSTTPPPPGPVLGAPVGYPPAAVYPPAPPPGYPHAPALYAPQPPPAAVAAASQQTAAQQQQQLQVFWAEQYREIEATTDFKNHNLPLARIKKIMKADEDVRMIAAEAPVVFARACEMFILELTHRGWAHAEENKRRTLQKSDIAAAIARTEVFDFLVDIVPRDEAKDAEAAVAAGMPHPAAGMPAADMGYYYVPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F2DDH2</accession>
    <name>F2DDH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">polyprenal reductase</fullName>
            <ecNumber evidence="4">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123427442</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="4"/>
    <dbReference type="EMBL" id="AK361939">
        <property type="protein sequence ID" value="BAJ93143.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK367198">
        <property type="protein sequence ID" value="BAJ98401.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967423.1">
        <property type="nucleotide sequence ID" value="XM_045111488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DDH2"/>
    <dbReference type="SMR" id="F2DDH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186670"/>
    </dbReference>
    <dbReference type="GeneID" id="123427442"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0154570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0154570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0154570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0186670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0186670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0186670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427442"/>
    <dbReference type="OrthoDB" id="541710at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000012">
        <property type="entry name" value="Polyprenol reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008954743" description="polyprenal reductase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="235"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAJ93143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0186670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="347" mass="38869" checksum="A2EB3C4C72327344" modified="2011-05-31" version="1">MEKGDGPALQPLLCLAWLAATLPIAAAALPIPAAAGGRFLHSLLAAFSSRGKTVRPSSSSKAKFTVPQKYFLHFYVVGVAVTTSLLLAICFYAYMKMTPLLAEPSSYSTIASHLIGSNSFSFGSVLSRTMEHKYRVWRTVFVLLLMEIQVLRRLYETENVFHYSPTARMHIVGYLTGLFYYTAAPLSLASSCLPEAIDFLRGQIAEFIVKGRARMPDLVIDSPSLIKPLLKLGWCQWIGAVIFIWGSLHQIRCHAILGSLRENKDSDEYVIPCGDWFSRVSCPHYLAELVIYLGMLIASGGSDISVWFLFIFVITNLSFAAIQTHRWYLQKFEDYPRSRYAIIPFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F2DH21</accession>
    <name>F2DH21_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Purple acid phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123405009</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="8"/>
    <dbReference type="EMBL" id="AK363188">
        <property type="protein sequence ID" value="BAJ94392.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK364911">
        <property type="protein sequence ID" value="BAJ96114.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044954820.1">
        <property type="nucleotide sequence ID" value="XM_045098885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DH21"/>
    <dbReference type="SMR" id="F2DH21"/>
    <dbReference type="PaxDb" id="4513-MLOC_10319.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623840"/>
    </dbReference>
    <dbReference type="GeneID" id="123405009"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0517650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0517650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0517650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0623840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0623840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0623840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405009"/>
    <dbReference type="eggNOG" id="KOG1378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="45007at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DH21">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.380:FF:000001">
        <property type="entry name" value="Fe(3+)-Zn(2+) purple acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000034">
        <property type="entry name" value="Fe(3+)-Zn(2+) purple acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF121">
        <property type="entry name" value="OS12G0637200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015019610" description="Purple acid phosphatase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="11">
        <location>
            <begin position="59"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="9">
        <location>
            <begin position="161"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="10">
        <location>
            <begin position="383"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ94392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0623840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="463" mass="52456" checksum="9D161BC4C0B38FCB" modified="2011-05-31" version="1">MGALGRHRCLNAALVLLAALLVRAEARGVTSAYRRRLEAAEDMPLEADVFATPPGHNAPQQVHVTLGDQAGTAMTVSWVTVDEVGNSTVMYGRAMGSLDMAAEGTHTRYKYHNYTSGFIHHCTLTSLEHGTKYYYAMGFGHTVRTFWFTTPPKPGPDVPLRLGLIGDLGQTSDSNSTLTHYEATGGDAVLFMGDLSYADKHPLHDNNRWDTWGRFSERSVAYQPWIWVTGNHEVDYAPELGETTPFKPFTHRYPTPHRSSGSPEPYWYSVKLASAHIIVLSSYSAFGKYTPQYKWLEAELKRVNRSETPWLIMASHSPWYNSYNFHYMEGEPMRVQFEQWAVDARVDLVFSGHVHAYERSHRVSNIKYNITDGRCKPVRDLRAPVYMTIGDGGNIEGLADSMTEPQPSYSAFREASFGHAILDIKNRTHAYYAWYRNADGVKVPADTKWFTNRYYMPNHDDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F2DH88</accession>
    <name>F2DH88_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123411468</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PDCD4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK363255">
        <property type="protein sequence ID" value="BAJ94459.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960350.1">
        <property type="nucleotide sequence ID" value="XM_045104415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DH88"/>
    <dbReference type="SMR" id="F2DH88"/>
    <dbReference type="STRING" id="112509.F2DH88"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703750"/>
    </dbReference>
    <dbReference type="GeneID" id="123411468"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0583780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0583780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0583780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0703750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0703750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0703750"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411468"/>
    <dbReference type="eggNOG" id="KOG0403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="414546at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DH88">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009646">
        <property type="term" value="P:response to absence of light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090549">
        <property type="term" value="P:response to carbon starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000008">
        <property type="entry name" value="Programmed cell death protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000009">
        <property type="entry name" value="programmed cell death protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039778">
        <property type="entry name" value="PDCD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12626">
        <property type="entry name" value="PROGRAMMED CELL DEATH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12626:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="140"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="304"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="438"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="601"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAJ94459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0703750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="720" mass="78752" checksum="47C348276239F51E" modified="2011-05-31" version="1">MASPRNEGGFLTQEQREKLRIAVQNAETLSLTSPRSPTGSTTSALLQQYELHQQQVRAAAAAARGGGGGGGGGGGARHARRSHSGKAVKLKKDGAGGKGTWGRLIDADTATFLDRNDPNYDSDEEPYELIEAPATTPLEDYKKSLVMIIEEYFSTGDVKQTGSDLKELGYDDFHRYFVKKLVSMAMDRHDKEKEMVSVLLSCLYGDGLSSTQIKLGFVMLLQAVDDLAVDIPDAVDVLALFIARAVVDDILPPAFLNKAKGSLTEASKGMQVLQIAAKSYLSAPHHAELLERRWGGSTHITVEEVKRRITDLLKEYIKNGDTAEACRCIRELAVPFFHHEVVKRAVTLGMESPAAETLIAKLLKEASEEGLISSSQMAKGFSRIVESLDDLSLDIPSAKSQFQTLVSKAVSEGWLDSSYEPSGANGNVQDDDHEKLRKYKREAVSMIHEYFLSDDIAELIRTLEELGLPEYNPVFIKKLITIAMDRKNREKEMASVLLSSLSMELFSSEDIVKGFIMLLESAEDTALDILDASDELGLFLARAVIDDVLAPLNLDEIGSILPANCSGAETLNMARSLASARHAGERLLRCWGGGTGWAVEDAKDKITKLLEEYESGGDVGEACKCIREMGMPFFNHEVVKKALVMAMEKKKEGTLALLHECFGEGIITINQMTKGFSRVRDGLDDLALDIPDARDKFLSYVENGKKNGWLVPSFGVAAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLH6</accession>
    <name>F2DLH6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small nuclear ribonucleoprotein Sm D1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">snRNP core protein D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123405972</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in splicing regulation. Facilitates post-transcriptional gene silencing (PTGS) by limiting the degradation of transgene aberrant RNAs by the RNA quality control (RQC) machinery, thus favoring their entry into cytoplasmic siRNA bodies where they can trigger PTGS. Does not participate in the production of small RNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AK364744">
        <property type="protein sequence ID" value="BAJ95947.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044955415.1">
        <property type="nucleotide sequence ID" value="XM_045099480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DLH6"/>
    <dbReference type="SMR" id="F2DLH6"/>
    <dbReference type="PaxDb" id="4513-MLOC_57708.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0588960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0588960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0588960"/>
    </dbReference>
    <dbReference type="GeneID" id="123405972"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0488180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0488180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0488180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0588960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0588960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0588960"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123405972"/>
    <dbReference type="eggNOG" id="KOG3428">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="TFLMKLT"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DLH6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01724">
        <property type="entry name" value="Sm_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000008">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034102">
        <property type="entry name" value="Sm_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAJ95947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0588960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="114" mass="12698" checksum="AAA8F352DB32E1BA" modified="2011-05-31" version="1">MKLVRFLMKLNNETVTIELKNGTTVHGTIIGVDISMNTHLKTVKLTLKGKNPVTLDHLSVRGNNIRYYILPDSLNLETLLVEETPRVKPKKPTAGKPMGRGRGRGRGRGRGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F2DR30</accession>
    <name>F2DR30_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123401190</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK366348">
        <property type="protein sequence ID" value="BAJ97551.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950855.1">
        <property type="nucleotide sequence ID" value="XM_045094920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DR30"/>
    <dbReference type="SMR" id="F2DR30"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545670"/>
    </dbReference>
    <dbReference type="GeneID" id="123401190"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0453610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0453610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0453610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0545670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0545670.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0545670"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123401190"/>
    <dbReference type="OrthoDB" id="666972at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF322">
        <property type="entry name" value="HMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ97551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0545670.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="159" mass="17599" checksum="7778C56DEFE4C6EA" modified="2011-05-31" version="1">MGILDAVTEMCACPRVRARRRMKKRPQLETVEMKVRIDCEGCERRIRKAVDGVRGVTGVEVLPKQNKVAVTGYIDDPARLMRRVARKTGKKVEPWPYVPYDVVPHPYAPGAYDKKAPPGYVRNVVADPDAAPLARASSAEVKYTSAFSDENPNAACAVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2DYM5</accession>
    <name>F2DYM5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123404629</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WRKY group II-e family.</text>
    </comment>
    <dbReference type="EMBL" id="AK368994">
        <property type="protein sequence ID" value="BAK00197.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044954505.1">
        <property type="nucleotide sequence ID" value="XM_045098570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DYM5"/>
    <dbReference type="SMR" id="F2DYM5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0606270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0606270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0606270"/>
    </dbReference>
    <dbReference type="GeneID" id="123404629"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0502650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0502650.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0502650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0606270.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0606270.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0606270"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404629"/>
    <dbReference type="OrthoDB" id="1937086at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000005">
        <property type="entry name" value="probable WRKY transcription factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF153">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="229"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="306"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAK00197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0606270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="488" mass="51196" checksum="CAC8CF5EFF83C6F4" modified="2011-05-31" version="1">MCDYYLQRVEGDQQAGDLTDIVRAGGAMPAGSADPPSTATEWLQLPTDPILFPPPQSSSDGAGPSGADADAFGDPFSGLPDPFGTDYPSSSGSAAADFFDAVQNAMDVGMAKQVGFVGTTGCGGGGTTAGAGGGFLDMRNHHMFPREMPMCGLSPRAMGPYALMGGGAAKLGVPMAGHGQTAGPCAFDSVAGLQMSSSPRGGGIKRRKNQARKVVCIPAPAAAVAGKTTGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRTDPNMLVITYTSEHNHPWPTQRNVLAGSTRSHYAKNSSNTAAASSKNSKNSSRTQHKPVVKAESKDLSATTQAVTSTTTTATTSTGNNTSPMPVKEEAEMERRIGGETPATVGYYSDHLLQQMFSQSYRPMTPEEAGGYHHQDDFFADLTELDSDPVSLIFSAEYMEARPDKEKAAAMDGVDSLFMTDWAPASAAAVTTSAGSSFEQGGIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0V0</accession>
    <name>F2E0V0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123449621</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Flower</tissue>
            <tissue evidence="6">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369771">
        <property type="protein sequence ID" value="BAK00972.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK375363">
        <property type="protein sequence ID" value="BAK06558.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044982837.1">
        <property type="nucleotide sequence ID" value="XM_045126902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0V0"/>
    <dbReference type="SMR" id="F2E0V0"/>
    <dbReference type="PaxDb" id="4513-MLOC_20532.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405350"/>
    </dbReference>
    <dbReference type="GeneID" id="123449621"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0337460.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0337460.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0337460"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0405350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0405350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0405350"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449621"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E0V0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030597">
        <property type="term" value="F:RNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAK00972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK06558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0405350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="328" mass="34373" checksum="C4219176F29B2831" modified="2011-05-31" version="1">MSKAPPREDPSPTGVAVSPEVEAALARGGAVVALESTIICHGMPYPKNLQTAMEVEAIVRENGAVPATIAILDGVPHVGLNSEQLKNLAISGSQFQKTARRDIAQVVASGGNGATTVSATMFFAHKVGIPIFVTGGIGGVHRYGEKTMDISSDLTELGKTPVAVISAGVKSILDIARTLEYLETQGVTVAAYRTNEFPAFFTETSGCKVPCRVDSPEECAKIIYANQNMHLGSGILIAVPVPKQHAASGKVIESAIQQSLQEADAKKIIGNTITPFILDRVKVLTGGSSLEANIALVKNNALTGAKIAVALSDLRKRVTNNRPVRSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F2EAA7</accession>
    <name>F2EAA7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase II subunit beta</fullName>
            <shortName evidence="2">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123450082</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Flower</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373082">
        <property type="protein sequence ID" value="BAK04279.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044983422.1">
        <property type="nucleotide sequence ID" value="XM_045127487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EAA7"/>
    <dbReference type="SMR" id="F2EAA7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0418710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0418710"/>
    </dbReference>
    <dbReference type="GeneID" id="123450082"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0349070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0349070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0349070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0418710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0418710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0418710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EAA7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF26">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAK04279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0418710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="254" mass="28441" checksum="133623042E87BB28" modified="2011-05-31" version="1">MDRKRINDALDRHLRTAAERPSPSTSRGAKPKPPSAAAPKPKEQLDSDSSDEGGSAGGSSEADDTFWINWFCAMRGNEFFAEVDDDYIQDDFNLCGLSAQVPYYDHALDLILDLEPVQEDVFADDQNELVVASAEMLYGLIHARYILTTKGLAAMLEKFKNCDFGRCPRVSCSGQPCLPMGQSDIARTSTVKIYCPKCEDLYYPRSKHQANTDGAYFGTTFPHMFMMTHSQLKPQKPAQQYVPRIFGFKVQKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECP1</accession>
    <name>F2ECP1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5 16">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC123394954</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Flower</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="16">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="5 16"/>
    <dbReference type="EMBL" id="AK373916">
        <property type="protein sequence ID" value="BAK05113.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945794.1">
        <property type="nucleotide sequence ID" value="XM_045089859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECP1"/>
    <dbReference type="SMR" id="F2ECP1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530510"/>
    </dbReference>
    <dbReference type="GeneID" id="123394954"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0441350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0530510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0530510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0530510"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394954"/>
    <dbReference type="HOGENOM" id="CLU_025154_1_0_1"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01570">
        <property type="entry name" value="NAPRTase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000002">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000006">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000155">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000227">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 16" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="18">
        <location>
            <begin position="30"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="17">
        <location>
            <begin position="328"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="435"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BAK05113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0530510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="561" mass="62051" checksum="C93EEA5B029F183C" modified="2011-05-31" version="1">MAAAALANGDVRAGGGGAVPRPTNPMVTPLLTDLYQFSMAYAYWKAGKHLDRAVFDLYFRKSPFAGEFTVFGGLEECIRFIANFKLKEEEINFLRTALPTCEDGFFEYLSSIDCSDVEVYAIPEGSAVFPKVPLMIIEGPVAVVQLLETPFLSLVNYASLVTTNAARHRLVAGKPKNLLEFGLRRAQGPDGGISASRYSYMGGFDATSNVAAGRLFGIPIRGTHSHAFVSSFLGLDEIIDKALSSSDGSNKCEDFVSLVQNWLVKIQDASSLRGSFGETNQSELAAFTSYALAFPNSFLALVDTYDVMRSGVPNFCAVALALNDMGYKAVGIRLDSGDLAYLSIETRKFFCAIEKEFGVDGFGKTNITASNDLNEETIDALNKQGHEVDSFGIGTYLVTCYAQAALGCVFKLVEINKQPRIKLSEDVTKVSIPCKKRCYRLYGKEGYPLVDIMTGQDEPIPKVGERILCRHPFIESKRAHVVPQHVEELLKCYWPGNSSVQREELPSINENRSRCMRQLDRMRPDHLRKLNPTPYKVSVSAQLYDFIHFLWLNEAPVGELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F2EIE9</accession>
    <name>F2EIE9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123440637</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Flower</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK375926">
        <property type="protein sequence ID" value="BAK07121.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973135.1">
        <property type="nucleotide sequence ID" value="XM_045117200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EIE9"/>
    <dbReference type="SMR" id="F2EIE9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307760"/>
    </dbReference>
    <dbReference type="GeneID" id="123440637"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0257120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0257120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0257120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307760"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440637"/>
    <dbReference type="HOGENOM" id="CLU_010119_16_4_1"/>
    <dbReference type="OrthoDB" id="406156at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="195"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK07121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="355" mass="38735" checksum="58634EED08149241" modified="2011-05-31" version="1">MELLSDCPAHTSVPDKYVLPPEKRPSVLDDGPSSHVVPPVIDLHGAAVSDGRRRHLIAAEIIKASKEFGIFQVVNHGVSEDVVQGFREAAAGFFAMPAAEKLPYRSDDLSKHFRVSSSTPYDRNGDRYWLDYLKITCHPVTDEHVREWPDKPGSFRGALAEYSAAVHELAQTLLRLIAEGLGLDDGFFAGDLSSGNTQMNVNYYPPCPDPSLTLGLLPHCDRHLITVLSQGDVAGLQARHGGRWLLVRPVPGAFVVNLGHQMEIITNGLLASVEHRAVTKADAARLSVVTLVMPKMECRIGPAPEMVNESTGPARFREFEFSEFIKAYSAAAASREDVLDYFRIHSTASSGSAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>M0VAY8</accession>
    <name>M0VAY8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Microtubule-associated protein MAP65-1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="M0VAY8"/>
    <dbReference type="SMR" id="M0VAY8"/>
    <dbReference type="PaxDb" id="4513-MLOC_21551.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0610000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0505800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0505800.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0505800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0610000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0610000"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="HKNISND"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VAY8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000911">
        <property type="term" value="P:cytokinesis by cell plate formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1520:FF:000002">
        <property type="entry name" value="65-kDa microtubule-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF9">
        <property type="entry name" value="OS02G0720200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="507"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="569"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0610000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="66232" checksum="08E3EA01FF5142FC" modified="2013-04-03" version="1">MDALQGQTSCGSLLQKLQLVWDEVGESDEDRDKVLFQLDQECLDVYKRKVDQALKSRDLLLQALDYSKMELARLASALGEKSIATSPEKTARTIKQQLAALAPTLEQLGKQKKERIKKFADIMSRIEQIRGEIAGNLEIGQQVPIPQINEDDLTDEKLRDFQSQLQELEKKKRERLKKVLEHVSTVQDLCSVLKMEHFSIITEVHDSLDDSVGKDHKSISNDTLSKLDRTIATLNKDKTLRLKKLQELATQLNDLWDLMDTPMEERSLFDHVTCNRTASVEEVTAPGALALDIINQAEIEVQRLDGLKYSKMKEIAYKKQTTLEDIYAGAHIVIDTAAAHDKIFALIESGNMEPTELIADMDNQIVKAKEEALSRKEILDKVERWISACEEESWLEDYNRDDNRYNSGRGAHLNLKRAEKARIQVNKIPGLVETLVAKTTSWEENHGLPFTYDGVPLLAMLDEYVMLRQEKEEEKRKMREQKRYTEQLLNIDREGPFGTRVSPYRVASAKKVPSPKPNGSVTNGSPGRRLSMSTQQNESKSSRSAGKDGKKAAAAAAPKTPASPNEAATAKEDDASIQHTDADPVPCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>M0VN30</accession>
    <name>M0VN30_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123404411</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044954272.1">
        <property type="nucleotide sequence ID" value="XM_045098337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VN30"/>
    <dbReference type="SMR" id="M0VN30"/>
    <dbReference type="PaxDb" id="4513-MLOC_34630.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005090"/>
    </dbReference>
    <dbReference type="GeneID" id="123404411"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0003910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0003910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0003910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0005090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0005090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0005090"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123404411"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VN30">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0005090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="574" mass="62485" checksum="60F2210022319B07" modified="2013-04-03" version="1">MGGKMEIERSWSEDGNLVQDAVDYRGCPADRSSTGSWLGAASVVGIELCERLATMGIAVNLVTYLTDTMHLPSADSANVVTDFMGTSFLLCFLGGFLADAFLGRYLTIAIFALVQALGTGLLAVSTTVHQLRPPPSEEATGLQMGVLYMCLYLIALGTGGLKSSVSGFGTDQFDERDDRERAAMGYFFDRFFFFISLGTLLAVTFLVYIQDHVGRSWAYGLCSGAMLIAIAVFLSGTKRYRYKRSSGSPVVHILQVLVAAARKRGLKQPHTAAALYEDRPEHARIHHTDQFRCLDSAAVMAGEEDNEMGPDGRPTPNPWKLCSVSRVEEVKMVARLMPVWATTILFWTIYAQMITFSVEQATTMDRRIGGFEIPAASLTVFFVGAIMLTLAVYDRVFIPLCRNLTGRPGFTNLEKIGIGLVLSIAGMVAAAVTEKKRLTVASTATTMNGTALPITVFMLIPQFLLVGAGEAFIYTGQLDFFITRSPKGMKTMSTGLFLTTLSLGFFLSSALVSLVRGATTWLGDTINHSRLDYFYWLLAVLGALNLAAYLLCAMWATPAAGSKAEQQPHPDEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>M0VR12</accession>
    <name>M0VR12_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123449182</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982233.1">
        <property type="nucleotide sequence ID" value="XM_045126298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0VR12"/>
    <dbReference type="SMR" id="M0VR12"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392850"/>
    </dbReference>
    <dbReference type="GeneID" id="123449182"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0326810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0326810.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0326810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0392850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449182"/>
    <dbReference type="OrthoDB" id="5914890at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0VR12">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000713">
        <property type="entry name" value="Ras-related small GTP-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24073">
        <property type="entry name" value="DRAB5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="37344" checksum="B171A074FE438089" modified="2013-04-03" version="1">MFWKDSSGRSSSSSGREQQSGVGPFGQVRVLVVGDSGVGKSSLVHLILEGSAIARPAQTVGCVVGIKHVTCGSAGGSSNNISNDAERNFFVELWDVSGHDRYKACRSIFYTQINGVIFVYDLSQRKTKTNLNKWAVEVAETGTFSAILGSGGPGGLPVPYLVIANKVDIVPSDGTRVRSGSIIDVARQWIEKRGLLPRSEELPLTESFPGNSGLISAAKEARYDKEAVTKFFRMLIRRRYFSNEPPAPSPWSLTPREDNILPVVTRKDDTDSFQRKSYSGEDFMFNGVPPLPAQRNLPPPPTLDPQLPVFSSDNNRYHRFSSPSLTEISNNRTSRENFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>M0WL09</accession>
    <name>M0WL09_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123412156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044961036.1">
        <property type="nucleotide sequence ID" value="XM_045105101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WL09"/>
    <dbReference type="FunCoup" id="M0WL09">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WL09"/>
    <dbReference type="PaxDb" id="4513-MLOC_52532.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0744530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0744530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0744530"/>
    </dbReference>
    <dbReference type="GeneID" id="123412156"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0617360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0617360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0617360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0744530.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0744530.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0744530"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412156"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WL09"/>
    <dbReference type="OrthoDB" id="617360at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WL09">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF64">
        <property type="entry name" value="OS12G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="278"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="524"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="6">
        <location>
            <begin position="791"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0744530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="990" mass="112215" checksum="8D44A62CE1A8725E" modified="2013-04-03" version="1">MAAAVTIGAAVGWGVSAVGWLASPIISRVLNKGFALLDFDATEKLKILDIQILQLQRVMEVVDESTYRVRLEPLLDKLKSALYEAEDILDALEYQRLKKQIHDGKSQDGSKVDSLKKNVRSVMSNPQNHKKSGMSKVELKESLEKIESAISDACKVLEQLKLLGVSNDNGSRAIATNSRSAVTTAGPPTRVIGRDQDRDKIIAMLNENKDQFQTNIVNGSGGASPGPFWALVRAFQKILRAYVLYWCSGLIAWFYRSEPTELGFATADAPSLSGTCYSVIGIHGIAGSGKSTLAQYVYEHEKKYKQEKMEGHFDIVMWIHVSQKFDLDSIFREMFEGATGKACDNFSSRNVLKEKLEDELRGKQILLVLDDVWYNIKNSGDREELQILISPLNVGKAGSKILVTSRTEAALVTLGAVKERCIPISDLDEKVFLEMFMHYALRDTRVSEHDRRILEMIGEDIAKKLKGSPLAARTVGSRLRETQTIEFWRSQKDRDLLNDTMGALWWSYQYLDEQVRRCFAYCSIFPRRHRLKRDELVKLWVAEGFIKTGKAEEEMEDVAKNYFDELLSASFLQLGGKDKLFEREVDYFTIHDLLCDLAEEVAGRDCFRIEKGCTGEVPKDVRYLFVATYDRELLTKKISGLQNLRTLIIDAYIEVKSNEYQVFASMFTTFMGLRKLRVLNLCLIGTGLRKFSFPDSIGQLKHLRYFLFWVTALTRLTVPAAFTKLYHMQVVDFGGCRDLAFSSSEDMMNLVNLRCVISEARLDFPDVGRLTWLQMLPFFTIRKKKGYEPHQLKHLNKLQGTLYICGLENVESKEEALEVNLAAKEKLTELVLQWKRGSCSPEVQAEVVEGLCPSKYLERLEIRNYLASGLPNWIVGMHSGSPKNLQELIFRNCGQLGPAGDLGAFVYLLSLCLVECSWDALPGNMDHLTSLKKLEITVCMNIRSLPTLPMSLEEFTLMYCNREFMRSCTTAADPNWQKIEHIPKKTIISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>M0WWL7</accession>
    <name>M0WWL7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123452156</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984692.1">
        <property type="nucleotide sequence ID" value="XM_045128757.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984693.1">
        <property type="nucleotide sequence ID" value="XM_045128758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0WWL7"/>
    <dbReference type="SMR" id="M0WWL7"/>
    <dbReference type="FunCoup" id="M0WWL7">
        <property type="interactions" value="2677"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0WWL7"/>
    <dbReference type="PaxDb" id="4513-MLOC_55259.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509400"/>
    </dbReference>
    <dbReference type="GeneID" id="123452156"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0423070.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0423070.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0423070"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0509400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0509400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0509400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452156"/>
    <dbReference type="eggNOG" id="KOG2034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0WWL7"/>
    <dbReference type="OMA" id="WIQREKW"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0WWL7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010015">
        <property type="term" value="P:root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16462">
        <property type="entry name" value="RING-H2_Pep3p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058919">
        <property type="entry name" value="Pep3/Vps18_RING_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26148">
        <property type="entry name" value="VPS18_RING_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="588"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="911"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="752"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="922"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0509400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="992" mass="111695" checksum="B7A42B9E0FA9D827" modified="2013-04-03" version="1">MDAGQLFSVDPLERQASRGHGAVTSMAAGSDVIVLGTSRGWLVRHDFSFEDALDLDLGGGRSGDHAVHRVFLDPGGKHCVVTVIHPGGAETYYHHARWPRPKPLPRLRGLLINAVAWNRQAITEASTKEVILGTETGQIFEMAVDEADKKEKYVKLLFELTEVREGIKDLQMETAVVGNATRYYVMAVTPTRLYSFTGIGSLETVFASYSDRAIHFMELPGEIPNSELHFFIKQRRAKHFGWLSGAGIYYGELNFGAQHSSSSGDENFVENKGFFDYSKLGDSNTKPASFAVSEFHFLLLIEDKIKVVNRISQQIVEELVVDNTPESSKGIIGLCSDASTGVFYAFDENSIFQVSTSDEGRDMWQVYLDMKAYAVALSHCLNSFQRDQVYLVQADAAFAAKEYYIAASFYAKMNYILSFEEISLKFISIGEQDALRTFLLRRLDNLTKDDKMQITMISTWATELYLDKINRLLLEDGTGATTNTVTNSNSSAYRSVVDEFRAFLSDSKDVLDEATTMILLESYGRVDELVYFAGLKEQYEIVVHHYIQQGEARKALEVLQRHNVPVDLVYKFAPDLIMLDAYETVESWMMARSKLNPGKLIPAMMRYVSEPHAKNETHEVIKYLEFCVKDLNNEDPGVHNLLLSLYSKKEDESQLLQFLDTKFGSGQANGPEFFYEPQYALRLCLQAKRMRACVRIYSMMSMHEEAVALALRVDLELAKAEADKVEDDEELRKKLWLKVAKHVIEQEKGVKRENIKKAIEFLSETNNLLKIEDILPFFPDFVLIDDFKEEICKSLKDYNSQIEQLKAEMDDATRGADNIRSDIGALAQRYTVIDREEECGVCRRKILTVGGLHQVGRSYTSVGHMAPFYVFPCGHAFHANCLIAHVTRCTSQVQAERILNLQKQLSLMDRKAAKDNGGTGNGEPTVSSTPSVDKLRSQLDDAVASECPFCGDLMIKEISLPFVLPGESAEKASWEIKPQRASQKILPMTMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>M0XA39</accession>
    <name>M0XA39_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123451188</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044984132.1">
        <property type="nucleotide sequence ID" value="XM_045128197.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984133.1">
        <property type="nucleotide sequence ID" value="XM_045128198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0XA39"/>
    <dbReference type="SMR" id="M0XA39"/>
    <dbReference type="FunCoup" id="M0XA39">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="112509.M0XA39"/>
    <dbReference type="PaxDb" id="4513-MLOC_58278.2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345160"/>
    </dbReference>
    <dbReference type="GeneID" id="123451188"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0288130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0288130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0288130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0345160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0345160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0345160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451188"/>
    <dbReference type="eggNOG" id="ENOG502R5WP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="M0XA39"/>
    <dbReference type="OMA" id="RIYIVRV"/>
    <dbReference type="OrthoDB" id="1900465at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF41">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="115"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="136"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0345160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="201" mass="21913" checksum="58B61828EAF9E26B" modified="2013-04-03" version="1">MDGSLVPDGNPNLSPTRFVKVFLHGEHFGRKINLTAHNSYDSLSFTLKRLGSNYSLSQYQLSGLEMSEEEGALHDDFMFLYDNMDGYRFFLGEVPWEDFTVSVKKIYIFPGEKQDDNEEYLEEGNGDNGDGNVAADGDDDATGEDGALDAVVAEDDGDGDEDEGDRAVDEDGDGAYDDEDRDVGDDEDDASSEGNDGGAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287EYF4</accession>
    <name>A0A287EYF4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alliinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123407837</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alliinase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045101067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287EYF4"/>
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    </dbReference>
    <dbReference type="STRING" id="112509.A0A287EYF4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0026390.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.1HG0026390"/>
    </dbReference>
    <dbReference type="GeneID" id="123407837"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0020910.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0026390.1">
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123407837"/>
    <dbReference type="InParanoid" id="A0A287EYF4"/>
    <dbReference type="OrthoDB" id="2020362at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.30">
        <property type="entry name" value="EGF-like, alliinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006948">
        <property type="entry name" value="Alliinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037029">
        <property type="entry name" value="Alliinase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF109">
        <property type="entry name" value="ALLIINASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04864">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alliinase C-terminal" evidence="4">
        <location>
            <begin position="76"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0026390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="440" mass="48091" checksum="65B9B5EE78DAEAA6" modified="2017-11-22" version="1">MAKDNGDGLAAIAGRIGVVGSVVLNVATLAIYLRGRFLLSAAAAAEKQASKKVKKAAAVAPSSGKPPVLPDSVIVLDHGDPTMFETFWRGPIGESATLVIPAWQTMSYFSDVGNLCWFLEPSFEREVRRLHRIVGNATTEGYHVLVGTGSSQLFQAALYALAPPTSDAPISVVSPTPFYSMYPFMTDFLNSGLYRWAGDANAFDGNDYIEVVCSPNNPDGGIHEAVLKSKSGKTIYDLAYYWPQYTPITHMLAHDIMLFTVSKCTGHAGTRIGWALVKDKEVAQKMNKFMELNTIGVSKDAQLRAAKILEAVVDGYERQPTTTAAVGGDANLLFHYARRKMTSRWRTLRDAVAASDIFSLPEEVAGFCTFTKDTAIANPAFAWLRCEKQEVEDLESFLRENKIITRGGTKFGAGRKVVRISMVDTDEAFGIFVDRLATMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287RLD0</accession>
    <name>A0A287RLD0_HORVV</name>
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        <recommendedName>
            <fullName evidence="3">F-box protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485420.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485420"/>
    </dbReference>
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0401940"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0485420.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0485420"/>
    </dbReference>
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    <dbReference type="OMA" id="CHFFTRE"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287RLD0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264:SF32">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264">
        <property type="entry name" value="OS07G0554500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0485420.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24664" checksum="6007EE7FA2D25FF2" modified="2017-11-22" version="1">METFLAPGEHAEDPLSFRVICLVQCRSNLLLLIFSSLDGQWHVLTYDQWGVCATLASFVKSEPGLSSRQFVHGCFYWRLHKRCELLVLNLRAMEFSAIKSRQRSDSFVVVEAAKGMLGMLTKGYGNDRCCLQYSILTNNQWHFEKVIPLPVKYVSLVGVAGGYLLVYAMYDASSQEKMKFGFFSVNLKTLQVELFAALGTHSPRRLYAGFPPSLCAPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287TEU8</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287TEU8"/>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287TEU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044952">
        <property type="entry name" value="SUV2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35761">
        <property type="entry name" value="ATR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35761:SF1">
        <property type="entry name" value="PROTEIN SENSITIVE TO UV 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0553180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="343" mass="38281" checksum="33D017C7AEB504E5" modified="2017-11-22" version="1">MYVDNNMEMERNNQEHSSALLNVPDPEEDGLHIGNMFLPSTFWTPFFSGVLQIALKYSEEGIRVDALSIMILIVRTSDSNGEREKFGFISVMESLHQLLQKENALLVKKHSVHLLFLLLNCPTMLKMLCSGGKDGSELMETVGCENDPQQAINSVLKDLSECLTCEATTSLELKLCRLVVNLLAYISSSGKSGYEVLLGSVTAHGASFLELTMEVLASQMDCKVDFSAEVHELLNERYLLLRETLILLNRLASHAMFSKPTLEVLMGSKRCAGLTIDIANRLPQRSKYPLRQLSEINPQMANGLAELAQKFRSRVYGFLEEQQHPTVDCRDTGASGKPPRVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287VAH0</accession>
    <name>A0A287VAH0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123406341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044955777.1">
        <property type="nucleotide sequence ID" value="XM_045099842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287VAH0"/>
    <dbReference type="SMR" id="A0A287VAH0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0634210.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.6HG0634210"/>
    </dbReference>
    <dbReference type="GeneID" id="123406341"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0526780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0526780.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123406341"/>
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        <property type="component" value="Chromosome 6H"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:CCR4-NOT core complex"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="probable NOT transcription complex subunit VIP2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="480"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="346"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0634210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="617" mass="66049" checksum="3A9879FA9DA9C940" modified="2017-11-22" version="1">MSGLLNSNLNGSTSNLPDSTARPFTTSFSAQSGSLQGFHHSALHNIHANYSLPNMPGSLAQRNAAMGGLPSSGVQQPGGRFNSNNLPVAMSQIPHAHSGAGARGMNVGGGQAFSSGMNMGGSIQGLSSNLGTSGNRNSVPGNPALGNLGQRITSSAGNIVGGSNIGRNISSGGLSVPSISSRIDFSGNAATGRLNVQGSNRMMNGLIPQGSSQLMNMIGSSYPTSGGSLSQNQMQPGNNSLGSMGMLHDSSDSAPFDINDFPQLTGRPNSAGGPQGQYGSLRKQGVGVNSIVQQNQEFSIQSEDFPALPGFKGGSSDYPMELHQKEQLHENVPVMQAQQYPMSRSVGFNLGSSYQPNRQQHQQGAANSIQSGGPQNIGLRPLNSQSQTSSLGSYEQLLQQYQQPQTQSPFRLQQMSSSAAQTYADHGLKPILGGRTPPDPYGLLGLLGVIRMNDPDLSSLALGIDLTTLGLNLNSPDNLYKTFGSPFSSEPAKGDPEYTTPACYVAEQPPALQPMHFQKFQTLILFYIFYSMPKDEAQICAANELYNRGWFYHKEARQWFTRIPNMEPLVKTPSYERGSYAFFDQGNWETVRKDNFVLHYELVEKRPSLPSASQIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287W6T5</accession>
    <name>A0A287W6T5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123407116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044956106.1">
        <property type="nucleotide sequence ID" value="XM_045100171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287W6T5"/>
    <dbReference type="SMR" id="A0A287W6T5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669240.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123407116"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.7HG0555300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0669240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0669240.1"/>
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123407116"/>
    <dbReference type="OrthoDB" id="2014495at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009768">
        <property type="entry name" value="MAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31246">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 70-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31246:SF4">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 70-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="355"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="570"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="46"/>
            <end position="122"/>
        </location>
    </feature>
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        <location>
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            <end position="343"/>
        </location>
    </feature>
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        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
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        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="471"/>
        </location>
    </feature>
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        <location>
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            <end position="492"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0669240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="607" mass="67646" checksum="ACF0973643E45EBA" modified="2017-11-22" version="1">MADGGEEGNAAVPRGFSRRRGAVRASLDADELMALMHGSDPVRVELTRLENELRDKERELGEAHTEIRALRLSERAREKAVEELTDELERMDEKLKLTESHLENKNLEVKKINDEKKAAMAAQFAAEATLRRVHAAQKDDDMPPIEAILAPLEAELKLSRQEIAKLQEDNRALDRLTKQKEAALLEAERTVQIAMAKAAMVDDLQNKNQDLMKQIEICHEENKILDKLHRQKVAEVEKLSQTVRELEESVLAGGAAANAVRDYQRKVQEMNDERKTLDRELARAKVTANRVAVVVANEWKDSNDKVMPVKQWLEERRFMQGEMQQLRDKLAVAERTARSEAQLKEKFQLRLKVLEDGLRGPPSGSSRPPTEGKSFSNGPSRRLSLGGADNVSKVSPNGLLTRRSPSFHSRSSLSSSSSLILKHAKGTSKSFDGGTRSLDRGKVHGNGSHLLNKSTDAVKDREADDGWKGTVDEGTNGSPSSNADQKTNETTNSNSIEMVSGFLYNMLQKEVVSLRKACHEKDQSLKDKDDAIEMLARKVDTLTKAMEVEAKKMRREVAAMEKEVAAMRVDKDQEIKARRLGNSKGSGSSQLLPGRSAPRSGLARNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6W903</accession>
    <name>A0A8I6W903_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6W903"/>
    <dbReference type="SMR" id="A0A8I6W903"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0080500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0080500.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0080500"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0097540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0097540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0097540"/>
    </dbReference>
    <dbReference type="OMA" id="NTHLHEN"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000465">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000870">
        <property type="entry name" value="OSJNBa0016N04.16-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF77">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="38"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0097540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="352" mass="39565" checksum="F07588C9BED72A92" modified="2022-05-25" version="1">MDHQSSTSRSDLERMLFDETAEPKALPLSLMAEITNGFSDKHKIGEGGFAVVYQGMLQNGKVAVKRLSNTHMYEKEFHREVECLMMVKHKNVVRFLGYCADTQGSMERYNGKFVMADVQQRLLCFEYLPNGSLDKYITDTSRKVQWRKCFQIIKGICQGLHYLHQRNILHLDLKPANILLDGNLVPKIADFGVSRCFHEMQSRAITVNICGTLGYLAPEFCNGEITYQLDICSVGVIIIEILTGKKGYLAVDNVVESWSNKLEKSQSDVQLKQVRICAEIGIECTDFNPAKRPGTKNIIDRLVSLDETDSMGGYIETSAVTSQQAENAPEELHQARPKVPGEISSEVSFVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WAH2</accession>
    <name>A0A8I6WAH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123425878</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCTP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044965598.1">
        <property type="nucleotide sequence ID" value="XM_045109663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WAH2"/>
    <dbReference type="SMR" id="A0A8I6WAH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0002100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0002100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0002100"/>
    </dbReference>
    <dbReference type="GeneID" id="123425878"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0002390.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0002390.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0002390"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0002100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0002100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0002100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123425878"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd08378">
        <property type="entry name" value="C2B_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04019">
        <property type="entry name" value="C2C_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08379">
        <property type="entry name" value="C2D_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047257">
        <property type="entry name" value="C2B_MCTP_PRT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047258">
        <property type="entry name" value="C2C_MCTP_PRT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047255">
        <property type="entry name" value="C2D_MCTP_PRT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013583">
        <property type="entry name" value="MCTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047259">
        <property type="entry name" value="QUIRKY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31425:SF33">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31425">
        <property type="entry name" value="PHOSPHORIBOSYLANTHRANILATE TRANSFERASE ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08372">
        <property type="entry name" value="PRT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="617"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="729"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="27"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="185"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="368"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0002100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="785" mass="87381" checksum="D33E9652154D6FEA" modified="2022-05-25" version="1">MASPGSSYNLVETKPPLPAKLGPRGAAMAATKMAGTYDMVEPMKYLYVSVVKARDLPTMDITGALDPYVEVKLGNFKGVTKHLVKNPNPVWRQTFAFSLANLQSNQLEVIVKDKDTVLDDFVGRVVLDVSDIPECIPPDSPLAPQWYILTDAHGGRFHHGHTLGEIMLAVWIGTQADEAFPEAYHSGAHPLSAEGLASTRAKVYYSPKLIYLKVSVIAARDLIGAENSNDPPVKPTIAKIQMGGQIRRTRPGQPPANPVWNDEFMFVACEPFEDPLVVTVEEKVAAGSDEPIGRIIIPVAANAPRNDLAKSVASKWFNLSRGMTVEQAAADVTTGTKNREHSKTFASKIHLKMSLETAYHVLDESTHYASDLQTSAKKLRKSAIGMLEVGILGARSLGGNKNPYCVAKYGAKWVRTRTLLGTAAHAWNEQYTWDVFDLSTVITVAVFNNKNLDGHGDAKDERIGKVRVRLATLESDRVYTHYYPLVSLTPGGLKKTGELHLAVRFTCTAWANMLAQYGRPLLPKMHYTHPISVGQLNSLRFLAMQMVATRLGRAEPPLRREVVEYILDVESHMFSLRRSKANFNRTISLFSGALAAVKWFDGICKWKNPLTTSLVHVLFLILVCYPELILSTVFLYIFLIGVWNYRRRPRNPPHMDTALSHAEQAQPDELDEEFDTFPTSKPGDVVRMRYDRLRSVAGRVQTVVGDLAMQGERAQSLLSWRDPRATAMFITFSFIVAVVLYLTPFRVVAVLAGLYLLRHPRLRSKQPSAPFNFYKRLPAKGDMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WF32</accession>
    <name>A0A8I6WF32_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123426855</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transport of phosphate groups from the cytosol to the mitochondrial matrix. Mediates salt stress tolerance through an ATP-dependent pathway and via modulation of the gibberellin metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044966686.1">
        <property type="nucleotide sequence ID" value="XM_045110751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WF32"/>
    <dbReference type="SMR" id="A0A8I6WF32"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171930"/>
    </dbReference>
    <dbReference type="GeneID" id="123426855"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0142050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0142050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0142050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0171930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0171930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0171930"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426855"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000012">
        <property type="entry name" value="Phosphate carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="71"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="168"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="269"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0171930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="370" mass="38995" checksum="EBE906D44AEEFA50" modified="2022-05-25" version="1">MALSDSSRHALLPSFLYAAPSAAATTTPFSAAAGIGGRGLVAPADAAATRPAVWTRAPSEPSRKIEMYSPAFYAACTAGGIASCGLTHMTVTPLDLVKCNMQINPAKYKSISSGFGVLLKEQGAKGFFRGWVPTLIGYSGQGACKFGFYEFFKKYYSDIAGPEYAAKYKTLIYLAGSASAEVIADVTLCPMEAVKVRVQTQPGFAKGLSDGLPKFVKAEGYAGLYKGIVPLWGRQIPYTMMKFASFETIVEMIYKYTIPVPKSECSKSLQLGVSFAGGYVAGVFCAIVSHPADNLVSFLNNAQGATVGDAVKKLGLWGLFTRGLPLRIVMIGTLTGAQWGIYDAFKVMVGLPTTGGVAPTPAAAGEQLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WFD8</accession>
    <name>A0A8I6WFD8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426982</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044966840.1">
        <property type="nucleotide sequence ID" value="XM_045110905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WFD8"/>
    <dbReference type="SMR" id="A0A8I6WFD8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174770"/>
    </dbReference>
    <dbReference type="GeneID" id="123426982"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0144490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0144490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0144490"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0174770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0174770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0174770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426982"/>
    <dbReference type="OMA" id="IKRTSHQ"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.10:FF:000005">
        <property type="entry name" value="RNA binding (RRM/RBD/RNP motifs) family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF36">
        <property type="entry name" value="RNA BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="514"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0174770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="692" mass="76192" checksum="6A8C14062251D92E" modified="2022-05-25" version="1">MDGGERTFKANFSAEGVALLRQRVRAKLHELMGDYTDDTLVEYVVVLLRNGRRKDEAAKELLVFLGDDNNAFVSWLWDHLSSNLHLYVQSKAVSANDEAKNTRSTARGLPLHNSSSNAQTSRVPEAETQKTTRVQPKREWGGIVREQSDAVPLRSVVAPVSHAEEKTNHTQAVRRTRSPDMHNHRKRGREEDTRPVKKTSHEVIGAPRRLLQFAVRDAVRLVQPPTPRSESSSKRLRSVVSTVESDSAANGRLQKMKSDVRVPGATAAFRAAAEAAEDVLKDRYSSESVFDRLGRRPLLATTEEPFDFREKDPKYGEYEHIDNVRAENQVEFHDRNQYVAKDTCMYDSGTEKAADSAFDTDRYDDTGAVRYNGVNPYQNKLSSSGGKESSVKWYNKAEGAAGVRSRRSIAQDTRASSGPRPSEKILNISVNNNTRNPPYHEIARNAGTFEHQVPIGKKDVGSIKSNATVAHAKVTHMTDKSKDLVHSSSLVEATKASSVVGGSTGQPESAPDSRTVFVSNVHFGATKDGLSCHFNKFGAVLKTLIVSDGATGQPTGSAYIEFLEKESAEKALTLNGTSFMSRILKVVRKSSVEVPQQPAWSRGVRASPFASRLIRTAYPRPTFPGAMRGRLAIRGNARSFQWKRGTADSVDAGKPSQATPVTPGNQMVTPITRSFTYTRTEPKQDVGATANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WI17</accession>
    <name>A0A8I6WI17_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WI17"/>
    <dbReference type="SMR" id="A0A8I6WI17"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128920"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0128920.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0128920.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0128920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0128920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="230" mass="24725" checksum="676D80F727E910DA" modified="2022-05-25" version="1">MPHLYDLYGMAHTASYKKAKAFSESDLDDPSNFTNISSHQKLVAYRDAGKATKGDDFNPSQEPLDPELVMISGGGRPHGSIAIGDGIIRCPLTLSEIKARQSSRCPEITPRPRLVELAIEAALHKERAANQAALEKEKLASQAALQAALDERDQTTTRLLEEERARNEAGQRALYELFVGLCEKSGQVPPPMPVFSSIGTNNSRAASHDPSPGQPSPNEAGASNRGASPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJJ2</accession>
    <name>A0A8I6WJJ2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJJ2"/>
    <dbReference type="SMR" id="A0A8I6WJJ2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0054780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0054780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0054780"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0043050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0043050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0043050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0054780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0054780.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0054780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001788">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g25270, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000285">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF515">
        <property type="entry name" value="UMP-CMP KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="310"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0054780.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="37889" checksum="46CBFF7344F70DEA" modified="2022-05-25" version="1">MDEEGAPRDGFTFESALRACSGARSAELGRAVHRDALTAGLAADVSVCNALVEMYAQCGDMEMACQVFDAMPERDAVSWNVLLGGLLGHGGLSTQATEAWRRMLGEGHKPDSVVLSAMLWHHPGDGKQGREVHAWVIRHWLETELSVANALIGMYSRKNELGHALSVFESMPVRDLASWNAILSAHSQDFRVLMMFRRMVDSGARPDETTFMAVLSACDNLGLVEGGMRLFSEMENKYRIQPTVQHCTSLVNMLGKAGMVREAYEFMSKRACLGNEPTVLRALLHASSVHGNIRIGEISAEKLFDLEPENAHNFVTLMKMYENTGRLEDLEKVKKMMRDKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WK13</accession>
    <name>A0A8I6WK13_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123429170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WK13"/>
    <dbReference type="SMR" id="A0A8I6WK13"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0083570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0083570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0083570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0101350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0101350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0101350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="585"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0101350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="684" mass="75329" checksum="287891817E19BD88" modified="2022-05-25" version="1">MGSNMISAGRISGFVEGQCVSDREKLLLSWTQRSHSLVVWLAYLYGRPLLLSFPRYCSCSYPRSHSRTYCSLPPTSSFSLAACCMCGMDAANPDLPKQEEQDHGNGISIEKMTGESPGDAVHNYRGWKAMPYVIGNETFEKLGTIGTLSNLLVYLTAVYHMKSVNAATLLNFFTGSCNLATVLGAFVSDTYVGRYTTLAAATIASFIGMVLLTLTAALHSLHPPACISKGQCESPIPSQLAVLLLSFFFLVIGAGGIRPCNLAFGADQFDPHTADGRRGMTSFFNWYYFTYTIAMMVSATVIIYLQSDVSWALGLAVPAVLMGLSCAVFFMGTRLYVRISPEGSPFTSFTQVVVAALRKRHLRPDRNTAELFDPPHRSKLVSKLAYTDQFTWLDKAAMETTEDVACSNGKTLVDPWRLCTMQQVEEVKCLARVIPVWSSGIVYFVVITQLGTYIVFQAAQTDRRISNSASFEIPQGSFVVFQMLALTLWIPVYDRLVVPALQRFTKREGGITQLERIGVGLVLSVVTMLVSAAVEHRRRRTAAMMSCFWLVPQQLLVGLSEAFTVVGLTEFYYRQFPENMKSVAGALFFLGVAVASFASGLMVTVVHRVTRRRDGRPDWLAQDLDEGRVDLFYLVTAAIAAANLVYFVACARWYRFKKSDGDVNAGNDAELDESSKKAASAAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WL40</accession>
    <name>A0A8I6WL40_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123447507</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980053.1">
        <property type="nucleotide sequence ID" value="XM_045124118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WL40"/>
    <dbReference type="SMR" id="A0A8I6WL40"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078480"/>
    </dbReference>
    <dbReference type="GeneID" id="123447507"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0063860.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0063860.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0063860"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0078480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0078480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0078480"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447507"/>
    <dbReference type="OrthoDB" id="599181at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF275">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="597"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0078480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="725" mass="83447" checksum="69BD341ED5D9D71A" modified="2022-05-25" version="1">MDGYAIIPADQEQSVIAADWSAGVLSDELEMHENSADFSPQLAEEQNQLYILGVNEMPINDTGSASDSDGSSENETDKEPGKYFYPGFEELVNPRPPEVGMRFPTLEDANRYYSAHALLTGFVAIRGPNFMRKKFHIECNRSRKLAPSQDLKRKREVYSVNKAQCEARVVVKPVKGQWEYTTVRSEHNHPLCPSPSLTRFFLSCKHMSAEERSFLRALQQSSIHPKKAMKIFKRMRGILGNLPFKTKDANTSQCAEQQEKPNSDVETTVNHLKELELQNPFVSFTTQIDEDSVVRSLFWTDARSRIDYEIFGDVLSFDTTYSTNKHNMPFTSITGMNGHGRTLVFGCALLQDKKAETFKWMFQTFLHVMGGEMPRAIVTDQDEAIKKAISEVMPQVKHRFCKFHVMRKAREKLGGFIMARGNINSELDALVNNSLTEKEFEEGWATLIERYSASGNEYLQLLWETRREWVPVYFREDFYPFAETTGCCEGANLLFKDYVLPKDRIEKFLERYEDMQERIIRIDDEDRLQSRTEPSCFSMQPIEKHAAHIYTRQIFLKVQRELLHSTAFNVDEIQPETVFRLQKVSNYENPEFDRNSFELLVEPGTNAIKCECAKFTRDGIPCCHMFRLFTQFGVNEIPGQYIVPRWTAKFKEEQMKQYKEKCLGSRGMDESENTMRYAMLLSKVTDIGKEICGDGSKCDKFSLELDAIEGKIGKATEETPETDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WLT3</accession>
    <name>A0A8I6WLT3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WLT3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001450.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF18">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001450.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="275" mass="31387" checksum="3AEDBBED5E226764" modified="2022-05-25" version="1">MATYIQSLLHAQLYISPEKPRLPEHPSHLLHLVHTYFHPTPTNGNGEIADPPKRRCQLLHRVRTYCSPPPSADPQRDMGRTDPYTGRWRRATEYCMHANVQFRCRDFAANVTCILDVRLQGGTLDIPCLHVGSGTWTLLRNLMALEEQMPKRPVTAYCIFMSQVACTVEDVRLLVEAKIIQHWQGSDKIAAQGFANLCNGVAMDVHDKNKNYLKPIWHNLEKLCDSKARNFKGSFRQKYCSTTLQQVVFGVTAFLALCQLLQSIYAPIAYHFPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WNR4</accession>
    <name>A0A8I6WNR4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WNR4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160260"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0160260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0160260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0160260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="233"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0160260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="319" mass="36299" checksum="858B2A14FAEA4D59" modified="2022-05-25" version="1">MFAATVSVLRSVATNRSATRNSRGDAGGSLKILITFDFVFILHLMERIMKITDVLCVKLQNKSLDIANALDCVSNTKELLAGLRDNGWDNLFEDVKSFCVKHDIDILETDQKYVDVTKSRNKHDNTTVLHHYKVDVFNVAINQQVIELNDRFSSQVTELLDLCSSLDPRHDAFDMSKICRVVDKFYPADFSSQERDRLECELPHFQLDTFNHPQIKNCKSLDDLTKGIVKTGKSSDYPMVERLLQLVITLPVSTATAERAFFAMKLVKTRLLSKMGDDFLCHCTLVYIEKELAAKFSPEEIIDIFDNGARKAEFKLVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WP42</accession>
    <name>A0A8I6WP42_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123409142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044958058.1">
        <property type="nucleotide sequence ID" value="XM_045102123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WP42"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0038720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0038720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0038720"/>
    </dbReference>
    <dbReference type="GeneID" id="123409142"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0030040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0030040.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0030040"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0038720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0038720.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0038720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123409142"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053052">
        <property type="entry name" value="Imprinting_Balance_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45496">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45496:SF2">
        <property type="entry name" value="OS10G0378450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0038720.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="203" mass="21834" checksum="41B7EF888DA238E4" modified="2022-05-25" version="1">MGHFAGPSAFASADGAFWTACPHCCYTHSYPRLYVGRRLRCPTVTCRRVFSADELPSTPPIVPGADMYFCTWAFFPLGPPAVAEGWAPFTPFNPAPPPSPSPVPNPTAAIPGCAIPTRVRPTSRKKVGVCLKGRARVEAEQEEEEEEEKASTVANLKAGEEVQADWLTLGDNGGSSGITINETVDLSELGFRVDESGFLHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WR28</accession>
    <name>A0A8I6WR28_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WR28"/>
    <dbReference type="SMR" id="A0A8I6WR28"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0152250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0152250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0152250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0183960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0183960.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0183960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000396">
        <property type="entry name" value="(+)-neomenthol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF64">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0183960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="391" mass="43216" checksum="1D3978BA7AAE0511" modified="2022-05-25" version="1">MCREEIRLFSHKREPAAQRNLHLQRGPSCSENKKKKTQPGPSEKPCEKKGKVHDASSIHHLLQACWLQSSHSLRQRGKIKMEAAISTNPSSKRTALVTGGNKGVGLETCRQLASKGLRVVLTARNEARGLEAVEGVRRSGAGGQADVVFHQLDVTDPDSVTRLADFVRDQFGKLDILINNAGISGVDRDPVLVAKVKEQVESMDVDQRVQWMKENSKETYEEAKECMRTNYYGAKLVTEALLPLLQLSSSGRIVNVSSGFSLLRNFNSEELKNEFNDVDNLTEKRLEELLDSFLEDFKANLIEAHGWPTGGSSAYKVAKAALNAYTRILAKKYPRMRINCLTPGYVKSDMSMHMGVLTPEEGASNPVKVALLPDDGPTGAYFDRDGVASFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WSF0</accession>
    <name>A0A8I6WSF0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SANTA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WSF0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0156590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0156590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0156590"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0156590.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0156590.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0156590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053090">
        <property type="entry name" value="Centromere_KNL-2_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015216">
        <property type="entry name" value="SANTA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35311">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN KNL-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35311:SF10">
        <property type="entry name" value="OS04G0347900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09133">
        <property type="entry name" value="SANTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANTA" evidence="2">
        <location>
            <begin position="70"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0156590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="488" mass="53972" checksum="075DE519E12BA954" modified="2022-05-25" version="1">MARKTRNPPPRARSRRGAPPPPPSPPPTPPPSSSTAAPALSPPFSPVRFCGRLGAAEAAAAAADVEHPHVTLWEWWPVRLKGEERKLAVSGFTEKNYLFTSAPIAQRYESLTLQDEDGVVVLLYGSFSLLRMRENGFSMQICEQFMIGFPYWWETWDSHMESYPNLFIHPQEDSAQFYLEKFQLGNFIQKFAPFLIKDLNDAKKLPINDLDAFIGGSRFQEHNCGNDVSTKENSAASDDARPAAVANVEIGLNPSSTSHERDHVNIEGNVSLAPTETYIGDETCKEAGNQNDTMHPDAREDDAGSHLFNSDWTCTMSPNHMPNDSEGGNATNAELVALDPLARVPPKSSNCCSEIAGAFQSVEPLSYQSAPVASLKNQQYLERNEHITLTQKAVSNKNVPSSIHSDVQSPKKTVGSAKKQRSAKQGLGRPTRLNLAPHAWLTRSRVRALSISTPESLKMRRTRSGRVIVPQLDSTRSWVVYDRSKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WW85</accession>
    <name>A0A8I6WW85_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Impact N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123430878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044970646.1">
        <property type="nucleotide sequence ID" value="XM_045114711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WW85"/>
    <dbReference type="SMR" id="A0A8I6WW85"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197890"/>
    </dbReference>
    <dbReference type="GeneID" id="123430878"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0164360.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0164360.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0164360"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0197890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0197890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0197890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430878"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF20">
        <property type="entry name" value="IMPACT FAMILY MEMBER YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0197890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="248" mass="26682" checksum="5AC29C147F6E94FB" modified="2022-05-25" version="1">MAAVRLSPRLRSIPLLLPGLDPAAAVHRSSATAAAARAMSSTSSSSASSAPTPYTTLVGRVRCEREIKRSKFIAVAASVPDERSAMSFLNEVKDPRATHNCWAYKVGEQFRYNDDGEPSSTAGKPIYSAIISSGIDMVMVVVIRYFGGIKLGTGGLVRAYGGVASECLKDAPTCLVKPKARVGMEVPFDLLGTVYNQLQHFHAEDIKQDYDTGKDGTVVVMFKVEYEKIESLGSAVNSACSRKIELLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WZN4</accession>
    <name>A0A8I6WZN4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WZN4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0231350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0231350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0231350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0279190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0279190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0279190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0279190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19521" checksum="2CA8562BA8FB7BC0" modified="2022-05-25" version="1">MPTATASNMFDEMSQSLDDEAFLHTTNVANDDFVSHEYVSQEYEIQYGVDNLDMDDEGFVAKGRSGNYTNAEDVLIRTAWKKVSQDASIGSDQTVNTYWQCIKDYFDERNTSGHFRSSDSIRQRWSTINAECQKWVGCLSNVARMNPSGCGDSDLANISSSCVFFQILDCFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X3T5</accession>
    <name>A0A8I6X3T5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X3T5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0227840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0227840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0227840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="9"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0227840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="562" mass="64452" checksum="2AA6790CA20B842D" modified="2022-05-25" version="1">MLDWYKARKEEVRIAFDELCPGNAQMISSTIQKQLAKSCGDAVRRAIKQEMGDNLFSVLVDESRDISVKEQMALVVRYMNKGEVIERFLGIKHVKETTSEALKKALVEVLGKNGLLIANLRGQGYDGASNMRGEFNSVQKLVRDENPYAFYMHCFAHRLQLVVVAVSRCCSSIEDFFEYVTLIVNAAGSSCKRKDILLDQQRINLLAKLESGEIFSGRGKNQETSLARPGDTRWGSHYKTLARIESMWDAVIEVLTIVHEDERNPSRAGGLVQIMESFSFVFIMKLMLQILRITNQLSQLLQRKDQNIVQAMSLVTDVKSSLANLRNHGWEPLFEDVKIFCNANDIPVPNMDEVVPRFGRSRKGGRNNVTQEHFFRVDTFFAAIDSITTKLDHRFNDMASELLCGFACLDPRDSFSKFDLDKVAKLIDIYDADFSNDDRKRIKIELELFINHMRRHDDFRVCHDLATLAKKMFKLEKNIMFPMVYRLIELGLLLPVATASVERAFSAMKIIKTDLRSKMSNGWLDDLMVCYIEREIFKGIDLAEIKKEFQHKGRRMPLPRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X443</accession>
    <name>A0A8I6X443_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdenum cofactor sulfurase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123439596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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                <consortium name="EnsemblPlants"/>
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            <strain evidence="2">subsp. vulgare</strain>
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        <source>
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                <person name="Muehlbauer G.J."/>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="21" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="21">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS04G0616500 PROTEIN"/>
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        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
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            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
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            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
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        </location>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="918" mass="100066" checksum="08205D89A80FE8BC" modified="2022-05-25" version="1">MDAVGQIPEELRNLTSLTNLNLGQNYLTGPLPSFIGELTNMQYMSLGINALSGPVPKELGNLTELVLLSISSNNFSGSLPSELGNLPKLDQLYIDSAGLSGPLPASLSKLTKMKTLWASDNDFTGQIPDYIGSWSLTDLRFQGNSFQGPIPATFSNLNQLTNLRIGDILNGSSSSLAFLSNMTSLSTLVLRNCRISDKLSSIDFSKFASLNLLDLSFNNITGQVPQTLLNLSSLNFLFLGNNSLSGSLPSSVGSSLKNLDFSYNQLSGSVPSWAKDSQLNLVTNNFVADSSSNSGLPTGWGCLQRDTPCFLGSPQSSSFAVDCGGKTSISGSDNSVYEPDDASLGAASFYVTGAPTWGVSNVGQFVDASNGSYIIYSSHQFQNTLDTELFRNARMSPSSLRYFGIGLENGNYTVTLQFAEFDFTDGQSWKSTGRRVFDIYVQGMRKEQNFDIRRAAGGKSYTAVRKQYIVPVMKNFLEIHLFWAGKGTCCIPSQGYYGPAISALSATPNFTPTVRNAAAKKNGNKTGVIAGVIVGVAVLGLVALAGILVWRQRQKRRKLSLEQEELYSIVGRPNVLSYGELRSATDNFSPNNLLGQGGYGSVYKGKLTDGRFVAVKQLSEASHQGKKEFATEIETISRVQHRNLVKLYGCCLEGNKPLLVYEYLENGSLDHALFGKEKSSLDWPTRFEICVGVARGLAYLHEESSIRVVHRDIKASNILLDANLNPKISDFGLAKLYDDQKTHVSTKVAGTFGYLAPEYAMRGHMTEKIDVFAFGVVVLETLAGRPNYSTEDENKIYIFEWVWELYENNHPLDMVDPRLEEFDSEEVLRAIKVALLCTQGSPHQRPSMSRVVAMLTGDVEAPDVVTKPSYITEWQMKGGNTTYMSTDVSGEPSSGPMPNSPSSRTSSPFLSSVVDGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8F0</accession>
    <name>A0A8I6X8F0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8F0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0172290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0172290.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0172290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001954">
        <property type="entry name" value="Glia_glutenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33454:SF4">
        <property type="entry name" value="AVENIN-LIKE A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33454">
        <property type="entry name" value="PROLAMIN PPROL 14P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13016">
        <property type="entry name" value="Gliadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035263408" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207280.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="117" mass="13106" checksum="69DD55694D9D7A86" modified="2022-05-25" version="1">MRTMFILALIALAVTSSVAQLDTTCIEGYGQCQQQPQQMNTCAAFLQQCSPMSYVQSQMWQASGCELMWQQCCSRWPRSQSRIGARPSVAWHRSSCRSSSSSKGKVLASLSSRFRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8M4</accession>
    <name>A0A8I6X8M4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8M4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277620"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0229990.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0229990.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0229990"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0277620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0277620.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0277620"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0277620.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="66" mass="7294" checksum="14FDE37A304327E9" modified="2022-05-25" version="1">MKRANLVVPIITIAAIIRRQGHIRVLHCTIFKSSSDAHHSAADGDPHQLGHRVLRGTYDNDSSIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X977</accession>
    <name>A0A8I6X977_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X977"/>
    <dbReference type="SMR" id="A0A8I6X977"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0444250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0444250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0444250"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0367970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0367970.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0367970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0444250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0444250.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0444250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0444250.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="124" mass="13974" checksum="2DBCC3B37409C2F2" modified="2022-05-25" version="1">MEKMKDTPVPEGEEPKSDAEIVEEVLKTEVKQSTFLRNVGLKSSSNNSGKGTSDVAAHVRDLEQKLEWSELQAEVMQEQMVAIKLKVEEYEAARDNELELLRKKSQEQEEKLAHLMALFGAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9M5</accession>
    <name>A0A8I6X9M5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X9M5"/>
    <dbReference type="SMR" id="A0A8I6X9M5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0135580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0135580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0135580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0164420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130:SF13">
        <property type="entry name" value="GRF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48130">
        <property type="entry name" value="OS12G0467600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15977" checksum="9839C12674B75532" modified="2022-05-25" version="1">MTRCTDCPRIAPLKRLTSKEEKNGNFGHEFVKCESKPEGQIVKKCHHFEWMDDYIQWLQGLGLLDSRGDAIRKFNLPHDSAAPAAAARSEYPTVVDVELKTQLKKMNKNFKQVIELKKQSNLIALGILDLGIFYLMAISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XA29</accession>
    <name>A0A8I6XA29_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XA29"/>
    <dbReference type="SMR" id="A0A8I6XA29"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222760"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0222760.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0222760.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0222760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF75">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035154695" description="3'-5' exonuclease domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="81"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0222760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="251" mass="28788" checksum="B26C563B8FA10FCE" modified="2022-05-25" version="1">MKSTNRSTVAISLFLLLSRSPSLFFSSLLPFSSSTMPTSFHKVLAHGTTMLDVVYTNLSREVPFFLQQLKEKWFDNAVDHEKFLGLDLEYTGDQRGVAVIQLCFARHVLIFQWARSDKHCPELMDFLRSGITFASVDIRNDKLKMRHRFGIEIPAACLVDLQTVFRLRHDMTSMAHMAVALIDEGYGDMKTSFPKSQHKLWEKGPLDPINIEYAAKDAYVSYELYRKIRVVNYGQRHLEEGGHSDSDDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XFH5</accession>
    <name>A0A8I6XFH5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XFH5"/>
    <dbReference type="SMR" id="A0A8I6XFH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188430"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0188430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0188430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0188430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="43"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="276"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="565"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0188430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="69569" checksum="E9F32A351930638D" modified="2022-05-25" version="1">MSAMPGFVAAVPDRVYKLHTTHTPRFLGLDARQGGRNYSAGSGDGVIIGVLDSGVTPDHPSFSGDGMPAPPAKWKGRCDFNGRSVCNNKLIGARVFDTAAGAGNGTTSTGAPLSPIDEDGHGTHTSSTAAGAVVPGAQVLGQGRGTASGIAPRAHVAMYKVCGLEDCTSADILAGIDAAVADGCDIISMSLGGPSLPYPEDSIAVGTFAAAEKGIFVSMSAGNSGPNYTTLSNEAPWMLTVAASTMDRLISTRVRLGNGLYFDGESVSQPDAASTVFYPLVYAGASSTPDAQFCGNGSLDGFDVKGKIVVCERGNDVGRIDKGAEVVRAGGVGMILANQAIDGFSTIADVHFLPASHVSYHAGDAIMNYIKSAARPMAQIMFRGTVLGTSPAPAITSFSSRGPSMQNPGILKPDITGPGVSVLAAWPFQVGPPSLAGEHSGPTFNFESGTSMSAPHLSGIAALIKSKHPDWSPAAIKSAIMTTADSTDRSGMPITDEKGTAADLFALGAGHVDLDKAMNPGLVYDISPADYIGFLCGMYTGKEVSVIARRAVDCSAVKVIPDRLLNYPSISIAFPSSWNPMTPMMVTRKVTNVGEAPAVYYPQFDLPENAMNVTVMPSSLRFTEANQVKVYKVIVWPRTSGDEVVVQGALRWVSDKHTVRSPISVAFAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLS5</accession>
    <name>A0A8I6XLS5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 SUMO-protein ligase SIZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123395108</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable SUMO E3 ligase that may regulate Pi starvation responses.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044945978.1">
        <property type="nucleotide sequence ID" value="XM_045090043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLS5"/>
    <dbReference type="SMR" id="A0A8I6XLS5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0466170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0466170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0466170"/>
    </dbReference>
    <dbReference type="GeneID" id="123395108"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0385900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0385900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0385900"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0466170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0466170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0466170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123395108"/>
    <dbReference type="OrthoDB" id="682531at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15570">
        <property type="entry name" value="PHD_Bye1p_SIZ1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16792">
        <property type="entry name" value="SP-RING_Siz-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000027">
        <property type="entry name" value="E3 SUMO-protein ligase SIZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000241">
        <property type="entry name" value="E3 SUMO-protein ligase SIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031141">
        <property type="entry name" value="SIZ1/2_SP-RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF42">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE SIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="13">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="14">
        <location>
            <begin position="357"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="526"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="601"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="756"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="608"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="769"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0466170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="794" mass="87315" checksum="0176EDECC9FE247B" modified="2022-05-25" version="1">MKPESADDAVLAGCKGKLKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQLNGLTKKPAVEKETVLKIVDETFRKLHSPANSAAASANQIDSGQSVKPKKKLNGSAQKDVKVRCPCGNSMASASMIKCDNPQCNVWQHVGCVIISEKSAESVPQELPSSFYCDICRISKADPFWVTINHPLLPTSIAPSKIATDGSSTIQYLEKTFPLSRANREMLQKADYDIQVWCILLDDQVPFRMNWPLHSDMQINGIEVRVVNRQPTQQLGANGRDDGPVLTEYCKEGPNKIVLSGSDSRMFSLGVRIAKRRSLQEVLKLVPKEHDGEKFDHALARVRRCVGGGAEADNADSDSDIEVVADRVSVNLRCPMTGSRIKIAGRFKPCVHMGCFDLEAFVELNQRSRKWQCPICLKNYSLDNIIIDPYFNRITSLIQSCEDDVSEIDVKPDGSWRVKGGAELKDLTRWHLPDGTLSVATNIGSKINASIVKHEVKEESLSDQLGSRIKLGIRKNNNGKWEITKRGDVNLTQSSDGDHPEHFKNGNCITPTSNNDHEDTEDLEPGQYDYPMGNVHDLDSSPIDEHVPAVSREQDIIVLSDSDDDNVTVLSPNALNSSSADDTGDPFPPNPPETSGTCEEQPGGGLDEASFLTFSEDFDDLAQLSFWQYPSNPQDDPGLKFTKNLGEMQNNTANHQPLHEPAAAAAANLPEHGHNNCVDESQASVASNCVDESLITVKNASQKRRNPEDGMTALDASVLDDGLPGKRPSGSSSLPWQQRSVRPRLILAIDSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XLY5</accession>
    <name>A0A8I6XLY5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123447640</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M67C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044980185.1">
        <property type="nucleotide sequence ID" value="XM_045124250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XLY5"/>
    <dbReference type="SMR" id="A0A8I6XLY5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338900"/>
    </dbReference>
    <dbReference type="GeneID" id="123447640"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0282730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0282730.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0282730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0338900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0338900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0338900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447640"/>
    <dbReference type="OrthoDB" id="3640at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08066">
        <property type="entry name" value="MPN_AMSH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000020">
        <property type="entry name" value="AMSH-like ubiquitin thioesterase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000024">
        <property type="entry name" value="AMSH-like ubiquitin thioesterase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044098">
        <property type="entry name" value="STAMBP/STALP-like_MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947:SF18">
        <property type="entry name" value="AMSH-LIKE UBIQUITIN THIOESTERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="14">
        <location>
            <begin position="346"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="122"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0338900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="523" mass="58502" checksum="BEC82AADB303647E" modified="2022-05-25" version="1">MAMASPRQAGRHINIEGMARPVAVDHRISLPYYFRIADNLLRQANIYREEKNLIDLYIILLRYSSLLSETIPKHRDYHAFKSREKEFLKKGPHNSDKLLNVISELESLKPIVKQQIAELNRRAAEERNGPHETYAGNGASSRTDQLAASPYMMQPAFGSSTGPLQKSSSRWNHQEASQQGVEPDRHLVKSYASLPYPKDETLSRHSILGPNGLHGQWTRPVTGVKIQYPSYPELTQSNIPSLVPAILNQNDSHGPNTTSLDGLTNNNGDMQSVLSLDDGQWSLPVKETASVSPASFEEEFSQLNIRQPNPPPVLAQVHPERRPISPSRVADPRPGAATCDTGRFQNLHVPVALMESFLRLAEANTAKNLETCGILAGNLKKRTFYVTTLIIPKQESTSDSCQATNEEEIFEVQDKGSLFTLGWIHTHPTQTCFLSSIDLHNHYSYQVMLPEAIAIVMAPTDTTRKHGIFHLTDPGGMGVIHDCQESGFHPHEEPLDGTSIYEHCSHVYMNPTVKFDMIDLRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XMF3</accession>
    <name>A0A8I6XMF3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XMF3"/>
    <dbReference type="SMR" id="A0A8I6XMF3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258730"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0258730.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0258730.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0258730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF445">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="150"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0258730.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="260" mass="28400" checksum="07F28F9E019FFC10" modified="2022-05-25" version="1">MLARPYSTIRPGTFDNSTVTGILEYRNPGSPSSSSFDKALPIFKPMLPYYNDTNLITNFTTKLRSLATKQYPAAVPQSVDRRFFFTIGLDTLLCPKNMTCQGPNGTQFAAAVNNVSLVLPSTALLQSHFTGLTSGVYAANFSVMPLSPFNYTGTPPNNTNVATGTKLLAVSFNTSVELVMQDTSILGIESHPLHLHRFNFFVVGKGIGNYDAVNDPAKFNLVDHIERNTFGVPAGGWVAIRFLADNPSIRETQCTHKNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XN20</accession>
    <name>A0A8I6XN20_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XN20"/>
    <dbReference type="SMR" id="A0A8I6XN20"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0531100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0531100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0531100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0441750.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0441750.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0441750"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0531100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0531100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0531100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF12">
        <property type="entry name" value="OS05G0134500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0531100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29949" checksum="9BCF89CE4B108450" modified="2022-05-25" version="1">MVSWSEGSYSSSSSDSDISSRAPPSILCVEWTGLATDLSARCEHQAICEKYVAFQSVDSGRRFLGCAQKEGPKCGYVEWVDPEWPPQLKTTLGRMWDMYEDENKTRLSDNLANAEGIFKVLKEKENMEKDLRFFKLDFAKMVADKEQALAQLGNTQIALTDLKEELEKKKISDKSATNIHQVFRAKAEKELELIKQELELMNQERGETMKERDEVMQERDELKKEKKKLEYMIGDLFKHKEETKSKIRKVREILDEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XQ35</accession>
    <name>A0A8I6XQ35_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQ35"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0478320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0478320.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0478320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127:SF94">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="49"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0478320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="333" mass="37515" checksum="B3E7CA8421F86A83" modified="2022-05-25" version="1">MDTRSEAEKLKGPTVVSVSPFPLDPGLAPLLLFDYQHDGEASGGDTIFLYSIPKRQLLGRRADGFKGHRYWLTPQGWVLMLHLESHKSFLWNPSTLERISLPLDQENLLQAAEGSMCLLSLKPTDPDCVVLLADLAHELIYYCHAGGNQWFKHEHEHGQSFKAGLTTLGGKFYAYNIYHKHNQIVTLEFLPYPAFSTRGLLEDCSPTGYTMFDCCILEGRGELFVLSVCYHGISDRHIARIVVHKLDVSRGAWLKVDTLGDMVIFFDNCRGYGASFDAHQLGLRKRDCIYFLMSDDKALYVYDMKRGTTAMHNPGPSLRDSLVPQFLLPSAPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSK2</accession>
    <name>A0A8I6XSK2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectate lyase</fullName>
            <ecNumber evidence="3 8">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123405168</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="3 8"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSK2"/>
    <dbReference type="SMR" id="A0A8I6XSK2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0566890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0566890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0469830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0469830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0469830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0566890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0566890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0566890"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00824"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF187">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342819" description="Pectate lyase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="9">
        <location>
            <begin position="125"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0566890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="388" mass="41487" checksum="88500271DA908EA0" modified="2022-05-25" version="1">MSVSGTMAILLFLFFTSMLSISYCSPLPVNAYGSDILVRRQQTLEHCGTGNPVDDCWRCDPGWADNRQRLADCAIGFGRNAMGGKGGRTYVVTNPGDEDPANPAPGTLRHGLVQNEPLWIIFACNMTIRPKQELLVSSHKTVDGRGASVVVGEGGACFTVRNGSNVIIHGITIRGCRPAPKSSAKTSDLSDGDGISLFGARDVWVDRCTLESCADGLVDVIEASTSVTVSNCLLTKHNKAMLLGHNDAFEDDRAMRVTVAFNRFGPGLVQRMPRCRFGVFHVVNNDYVNWGKYAIGGSASPTILSRGNRFCAGKEKEVTKRDGDSPKSEWQKWSWISDGDLMFNGAFFTASGGSGPEVKAPSFAKPASLVAPMTASAGALSCSKGSVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSN7</accession>
    <name>A0A8I6XSN7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123440565</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in ammonium transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044973064.1">
        <property type="nucleotide sequence ID" value="XM_045117129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSN7"/>
    <dbReference type="SMR" id="A0A8I6XSN7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303830"/>
    </dbReference>
    <dbReference type="GeneID" id="123440565"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0253580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0253580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0253580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0303830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0303830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0303830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440565"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000005">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF27">
        <property type="entry name" value="AMMONIUM TRANSPORTER 2 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="29"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0303830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="494" mass="52369" checksum="21E577CB54CB3C0D" modified="2022-05-25" version="1">MAAPPPMPGAYMPDLPAVPEWLNKGDNAWQLTAATFVGIQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASTLIVWVLLAFRMAFGDRLFPFWGKAGPALTGDFLVARASFPATAHYGAGGALEVPPTQPYYPEATLVLFQFQLAAITLVLLAGALLGRMNIKAWMAFTPLWLLLSYTVCAFSLWGGGFLYHWGVIDYSGGYVIHVSSGVAGFTAAYWVGPRLKSDRERFAPNNILLMIAGGGLLWLGWAGFNGGAPYAPNIIASIAVLNTNVSAAASLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITAGAGLVHTWAAVLMGICAGSVPWFTMMVLHKRSSLLQKVDDTLAVFHTHAVAGLLGGVLTGLLATPELTALHTHVPGTRGAFYGGGIQQVGKQLAAALFVVAWNVVVTTGIILGVGLVIPLRMPDEQLRIGDDAAHGEEAYALWGDGERFDVSRRAAARATEVGDQSVDQRLAAMGARGITVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XSW3</accession>
    <name>A0A8I6XSW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC123431380</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Secreted</location>
            <location evidence="2 14">Extracellular space</location>
            <location evidence="2 14">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the germin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044971118.1">
        <property type="nucleotide sequence ID" value="XM_045115183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XSW3"/>
    <dbReference type="SMR" id="A0A8I6XSW3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0217970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217970"/>
    </dbReference>
    <dbReference type="GeneID" id="123431380"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0180650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0180650.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0180650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0217970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0217970.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0217970"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123431380"/>
    <dbReference type="OrthoDB" id="601303at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000047">
        <property type="entry name" value="Auxin-binding protein ABP19a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035337835" description="Germin-like protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="15">
        <location>
            <begin position="61"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0217970.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="218" mass="23120" checksum="0BA83D8F772AD0DC" modified="2022-05-25" version="1">MAKAVLLLLLPLLLVLLPPPFGCVAQLAKEFCVADLASTDTPAGYPCKLQAAVTADDFYYRGLGTTGPIIQPFKISLSSAFVTNFPGVNGFGISAARVDLAVGGVVPLHWHPAATELIFVLEGTLSCGFISATSNRVYTNTLYKGDLMVLPQGQQHFQYNIGNTTAAALSTYSSHNPVVQTLDFALFANNLPTEVVSKVTLLDDLQVKKLKAIFGGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTC0</accession>
    <name>A0A8I6XTC0_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">VQ domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTC0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0517230.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0429810.1">
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0429810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0517230.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.5HG0517230"/>
    </dbReference>
    <dbReference type="OMA" id="YDAPAMV"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039607">
        <property type="entry name" value="VQ_8/17/18/20/21/25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33143">
        <property type="entry name" value="F16F4.1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33143:SF9">
        <property type="entry name" value="VQ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="2">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0517230.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="146" mass="14970" checksum="F666EAAC5C1278BD" modified="2022-05-25" version="1">MTAAMTMSGASSPSKKSGLRGPRPQPLKVASSPPSSRPSSKKAHGGGAPVIVYEQTPKVVHARPHEFMTVVQRLTGKPATAPALFPGCDVPVAPMEEGAGVGDPLLLTLGQHPAMAAGQLMSPGGFIFSPNTMQSIQELCSTPCAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XX75</accession>
    <name>A0A8I6XX75_HORVV</name>
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            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
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    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044949474.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XX75"/>
    <dbReference type="SMR" id="A0A8I6XX75"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0467490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0467490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0467490"/>
    </dbReference>
    <dbReference type="GeneID" id="123399116"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0387000.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0387000.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0387000"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0467490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0467490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0467490"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123399116"/>
    <dbReference type="OrthoDB" id="190958at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024767">
        <property type="entry name" value="PRP38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12871">
        <property type="entry name" value="PRP38_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 38 C-terminal" evidence="9">
        <location>
            <begin position="179"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="180"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
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        <location>
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            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
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        </location>
    </feature>
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        </location>
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            <end position="360"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0467490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XY07</accession>
    <name>A0A8I6XY07_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA gyrase subunit B</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123443487</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">A type II topoisomerase that negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16 12">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="12"/>
    <dbReference type="RefSeq" id="XP_044975802.1">
        <property type="nucleotide sequence ID" value="XM_045119867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XY07"/>
    <dbReference type="SMR" id="A0A8I6XY07"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0256040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0256040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0256040"/>
    </dbReference>
    <dbReference type="GeneID" id="123443487"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0212540.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0212540.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0212540"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0256040.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0256040.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0256040"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123443487"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16928">
        <property type="entry name" value="HATPase_GyrB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00822">
        <property type="entry name" value="TopoII_Trans_DNA_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000005">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000002">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000002">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000565">
        <property type="entry name" value="Topo_IIA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004189">
        <property type="entry name" value="PRK05644.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45866:SF1">
        <property type="entry name" value="DNA GYRASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45866">
        <property type="entry name" value="DNA GYRASE/TOPOISOMERASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01159">
        <property type="entry name" value="DNAGYRASEB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="503"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0256040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="722" mass="79853" checksum="1E9FBDFD9B8D2A0A" modified="2022-05-25" version="1">MALLLRPSPPPPHLRLLLRRLLSTAPAPSRLLTVPPPSARLPFRPRVVAAAVPGRNGVAVRAFMASTAASDAMQEKRVAGEYTAANVQVLEALDGVRKRPGMYIGSTGPRGLHHLVYEILDNAVDEAQAGYATEINVVLHDDNSVSVTDNGRGIPTDIHPQTRKSCVETVLTLMHAGGKFGGSTSSYSVAGGLHGVGLSVVNALSEALEVTVCRDGKEYRQHYSRGKPLTMLSSTTLPDESSSRQGSCIRFWPDKEIFTTTIEFDFSTISSRIRELAFLNPELTITLTKEEQDAQVQHNEYCYAGGLVEYVAWLNTDKKPLHDPIAFRKELDGIIVDVSLQWCSDSYSDTVLGYANSIRTIDGGTHIEGLKTSLTRTINNFAKKSKIMKDKDISLSGEHVREGMTCIISVKVPNPEFEGQTKTRLGNPEVRRIVEQSVQENLTEYLELHPDVLDSILSKSLNALKAALAAKRARELVRTKSVLKSSSLPGKLADCASSNPAECEIFIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIERRDEAAMYKNEEIQNLILGLGLGVKGEDFKKEALRYHKIVILTDADVDGAHIRTLLLTFFFRYQRALFDEGCIYVGVPPLYKVERGKQVHYCYDEADLKDLVNTFPTNASYNTQRFKGLGEMMPLQLWETTMDPERRLLKQLTVEDAVEANVVFSSLMGARVEYRKELIQKAASIVNLDHLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XYR6</accession>
    <name>A0A8I6XYR6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XYR6"/>
    <dbReference type="SMR" id="A0A8I6XYR6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0360310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0360310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0360310"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0360310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0360310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0360310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF111">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0360310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="194" mass="22536" checksum="AE6A8A67C855E293" modified="2022-05-25" version="1">MEFFMIILEKSSSRQVLPDNFVKILDDHRPENMKLRQADNGLGKPWDVEVVFDTDGSMYLDRGWKQFVRAYDLQHGCFLVFRYDDNATFAVKVFDTTMCRRRYQDDDDASNGSRSSEYCDRCYVYGNSDSGYSKSSIDSSYSKSSSDDGLAMVIPQLGDRAMPIVLEEYIPQSFLGLRRSKRIRMMKEKVKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XZK4</accession>
    <name>A0A8I6XZK4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6XZK4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276080"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0228710.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0228710.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0228710"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0276080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0276080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0276080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF649">
        <property type="entry name" value="OS01G0651000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035238967" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="32"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0276080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35960" checksum="BCF211573F9DA588" modified="2022-05-25" version="1">MAQERQGHRSISWCILVVVVVLLLSARGSSASCYKRIFAFGDSIIDTGNFVYSTGSAPNALKEFPYGMTFFHHPTGRVCDGRVLLDFYAQALGLPLVQPSLPEQRSGQCTFGANFAVFAATALPPQYFKRWNMDIPGSANLGMQMGWFKEVVKRIAPGPGARRLLGESLIILGEIGGNDYNFLFFGRNHTRETAYQFIPDVVNRIISVAQELVDLGARTIMIPGNFPIGCVPKYLNDYHTGNPADYDKFGCLRWYNDFSMRHNMALSNAVNRLMAQHPRVKLIYADYFGAVMEIFKNPHRFGIGDPLVACYDGGGRYHVGTCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0I3</accession>
    <name>A0A8I6Y0I3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123449053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044982066.1">
        <property type="nucleotide sequence ID" value="XM_045126131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0I3"/>
    <dbReference type="SMR" id="A0A8I6Y0I3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0389210"/>
    </dbReference>
    <dbReference type="GeneID" id="123449053"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0323770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0323770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0323770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0389210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0389210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0389210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449053"/>
    <dbReference type="OMA" id="LMKCGAG"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015711">
        <property type="term" value="P:organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015748">
        <property type="term" value="P:organophosphate ester transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="43"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="149"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="244"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0389210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="342" mass="36943" checksum="9529CDA2D25EAFB5" modified="2022-05-25" version="1">MQTEASVGMAAATVDGAAAVAARRYSTQQPQPQPQQHHNQPKLGTTLHLLAGGVAGAVSKTCTAPLARLTILFQVQGMHSDVATMRNTSIWREASRIVYEEGLRAFWKGNLVTIAHRLPYSSISFYTYERYKNWLQMIPGLDNSGGLGADVGVRMVGGGLSGITAASLTYPLDLVRTRLAAQTNTAYYRGISHALFAICRDEGPRGLYKGLGPTLLGVGPSIAISFSVYETLRSHWLLERPYDSPIFISLACGSLSGVASSTITFPLDLVRRRKQLEGAAGRANVYKTGLVGTFGHIIQTEGYRGLYRGILPEYCKVVPSVGLIFMTYETLKSMFAGGASDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0V6</accession>
    <name>A0A8I6Y0V6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EngB-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123444343</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044976966.1">
        <property type="nucleotide sequence ID" value="XM_045121031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0V6"/>
    <dbReference type="SMR" id="A0A8I6Y0V6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0286830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0286830"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENGB-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649">
        <property type="entry name" value="MSS1/TRME-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="11">
        <location>
            <begin position="143"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0286830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="327" mass="36906" checksum="E8C3D62D8C5BD37F" modified="2022-05-25" version="1">MLLCHRILPRLLSLPSSPASKLLIRPSRFPPRRPFLPRCAALSQLAAPQSADHSDAEESADHFDAEEPQEPQEPQEVQVKLPLDRLFLPPGASVTPGDEEVVTARVLKGSNIVLGTYARGDAQVVNADFIKSSVRPDDCPRDGLPEFALVGRSNVGKSSLLNSLVRRKRLALTSKKPGKTQCINHFKINDSWYLVDLPGYGYASAPQVARKDWDEFTRNYFLSRDNLVSVFLLIDASIPAKKIDLDYASWLGQNKVPMTLVFTKCDKRKKKKNGGKRPEENVECFQNLIREYFEAAPPWIMTSSVTNQGRDEILLHMSQLRNYWRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y155</accession>
    <name>A0A8I6Y155_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y155"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="12"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="355" mass="40624" checksum="F012034F5FAD782D" modified="2022-05-25" version="1">MTTWYELRSRLQTHQTIDKATQRQLESEKDRWRKVLFRIVCIVKFLSKHNIAFRGTNSKLYEDSNGNFLGLVEMSAEFDPVIQEHVRRITNEETHAHYLDHKIQNELIHLLASAIKSEIVKKIKSAKYFSVILDCTPDASHQEQMSIIIRYVDSSSSHVCIGESFLGFLDVNHTTGQGLFEMLENELKLLDLDIDDVRGQGYDNGSNMKGTHHGVQRRLLNVNPRAFYSTCGCHSLNLTLCDMAKTCGKAKDFFGIIQRIYTIFANSTKKWHILKENITGLTLKSVSATRWESHIDSVKAIRLQCADIREALLQVSEIDNDIKTSSEAKGLENNELREYEFIVAIVIWGIGKLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y172</accession>
    <name>A0A8I6Y172_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y172"/>
    <dbReference type="SMR" id="A0A8I6Y172"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396350"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0396350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0396350.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0396350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF189">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="6">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0396350.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="195" mass="22770" checksum="28A701A49BDD16D8" modified="2022-05-25" version="1">MPPRRRGASGYRGVRLRPNGRYYSEIRSGDLRLSLGTYETAHEAARAFDAAAWRLGRPRGQMHFQDVYTLQQALDVAPPPRLNTAQDRAKHAARQRRLLVAQEDERVMAEWRRRHPEDVACEQVYWARRREEDTRRRREERLDRHRRKALANAEAHIVAAGGRSFFTENDDRWLDIWLSTSDDTDDDDDGDDGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y3Q4</accession>
    <name>A0A8I6Y3Q4_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3Q4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0475520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0475520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0475520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0573600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0573600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0573600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF188">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0573600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="211" mass="23087" checksum="7F4BC8166372CE7B" modified="2022-05-25" version="1">MSSTPDPDAPPREVERRITYSRESLLSVREARRHKLPAAIGACKREVEAASHMWWESEPRGGSKSEPPRTSKSKKMCTRFFSAGDCPFGEDCRYLHRVPNGHHEPNQTVTKKMSSLDLGGDEAQPRTPVSATAPATVKTRLCKNHGAPDGCKWGDRCHFAHGDGELGKPMGPGSSFKTRLCVSFVAGGSCAFAGRCHFAHGKEELRRPDAS</sequence>
</entry>
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    <accession>A0A8I6Y3Z2</accession>
    <name>A0A8I6Y3Z2_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123396978</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_044947647.1">
        <property type="nucleotide sequence ID" value="XM_045091712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y3Z2"/>
    <dbReference type="SMR" id="A0A8I6Y3Z2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0494120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0494120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0494120"/>
    </dbReference>
    <dbReference type="GeneID" id="123396978"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0494120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0494120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0494120"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123396978"/>
    <dbReference type="OrthoDB" id="5210at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0494120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="398" mass="42221" checksum="101551D34A77447B" modified="2022-05-25" version="1">MAILGELGMEILIPVCGVIGIVFAVAQWFIVSKVKVTPGASSAAAGAKNGYGDYLIEEEEGLNNHNIVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQPCTYSKGTCKPDLYTALFSTASFFLGAITSLVSGFLGMKIATYANARTTLEARNGVGKAFITAFRSGAVMDFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITRYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNVPEDDPRNSAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPALKKQLIISTALMTVGVAVISWLALPAKFTIFDFGAQKEVSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y4C3</accession>
    <name>A0A8I6Y4C3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
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        <source>
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</entry>
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        <submittedName>
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                <person name="Mayer K.F."/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
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        </location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="24330" checksum="4E0F52351C98FA60" modified="2022-05-25" version="1">MYDLYAMAHTASYKKVKAFCESDLEHPENFTNISSHDKLVKYRDHGKARKGEDFNPSEGPIDPELVMIPGNGRPHGSIAIGDGLIRCPSTLRQIKACQTSSCPEITCRPRPVELAIEAAIQKERAAMQAAMAEKDKEIRELEERTTALVEAERARNEASTRAIYDLFVSMCEKNGQVPPSMPVINVAGTNMSRNASHDPSTVEPSPGQPSPGETSQSHRASP</sequence>
</entry>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0593480.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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        </submittedName>
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    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
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    <reference evidence="11" key="1">
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
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            <location evidence="1">Nucleus</location>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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</entry>
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                <person name="Muehlbauer G.J."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <source>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
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    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="934"/>
        </location>
    </feature>
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        <location>
            <begin position="515"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="593"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0542490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="934" mass="103890" checksum="642DB4D42CA68504" modified="2022-05-25" version="1">MKTMRLLCLLVLLLLWTATVRASAGVQGSRAGNRSALPSLAGCQKRCGNLSFDYPFGIGSGCFRPPRREFELFCNRTHGASQPPRLFLHDGITEVVADIKPDDRDVEPYIEVSFSQSVPVRPEEDAYSISWNHGRSPTSYSLQLNFIGCDFDMYVLEHGTNKTVGQCTTTCPDEDITDKVSSLRQDCNGTGCCSTYVRSESTTGFDIKLVRHKIGKLKFKAHSNRSSIWDTIDVSTDYASISWGITVDEAGGPFQNSTDYACLSNNSSSRLMFQYGPAQYYCDCDGGYGGNPYITDGCSRDKGYDPIQRKTNCSRLCGEISVPFPFGLEDGCSARMSFQLDCTNSSLSTLQFLNMNGDTGEITYVKSINIMKGLVNVKYSLYQEEYLSVRVHQDPDHYVAFAGVVPQQWAVANLTCLEAQANNTGYACVSMNSKCLAVNSEREYYGYRCTCMDGFDGNPYTISDGCKDVDECTKPGTCNGTCQNTEGSYHCTECPSNTVYDTATKMCTSTKKQNLVLGIVIGISVGFGIILLMSIVILLIRRWKKDIKKKLRRKHFRENQGLLLEQLILSDENASENTKIFSLSELEKATNNFDPTRIVGRGGHGMVYKGILSDQRVVAIKKSKVIEQIEISQFINEVAVLSQVNHRNIVKLLGCCLETEVPLLVYDFIPNGSLFGILHASTTSSSIFSWDDCLKIAAEAAGALYYLHSAASVSIFHRDVKSTNILLDGNYTAKVSDFGASRLVPIDQTHVVTNIQGTFGYLDPEYYHTGMLNEKSDVYSFGVVLVELLLRKKPIFTCDSGLTQNLSNYFLWEMREKPLAEIVATQVLEEATNEEINDVANLAETCLQLRGEERPTMKQVEMKLQYVRSRRLRSSQVVLKNEGMQPLSSGQSQDTLPMLTTPSGRVDRFNIASQRNQNCYSLEQEFMASATLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YGK5</accession>
    <name>A0A8I6YGK5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YGK5"/>
    <dbReference type="SMR" id="A0A8I6YGK5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0590810.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0590810.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0590810"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0712300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0712300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0712300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0712300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="135" mass="15893" checksum="C003C691F3524B2C" modified="2022-05-25" version="1">MQPERKKARVEQIEVRRQLKRNTPSKDSIAMENPEYVATEEEVRSSKLTTKHRNCVAGGKRQTLIHERNEEFTRRRKQTVAESSKEDKSMTETCDENNQPLEQPQVMTYGKNLDFILAFEFICRTNNLFWTDIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YK74</accession>
    <name>A0A8I6YK74_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Condensin complex subunit 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123449701</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982946.1">
        <property type="nucleotide sequence ID" value="XM_045127011.1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407720"/>
    </dbReference>
    <dbReference type="GeneID" id="123449701"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0339420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0339420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0339420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0407720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0407720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0407720"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449701"/>
    <dbReference type="OrthoDB" id="1884734at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055406">
        <property type="entry name" value="HEAT_Maestro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055408">
        <property type="entry name" value="HEAT_MROH2B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048465">
        <property type="entry name" value="Maestro-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045206">
        <property type="entry name" value="Maestro_heat-like_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056282">
        <property type="entry name" value="MROH2B-like_N_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120:SF0">
        <property type="entry name" value="MAESTRO HEAT-LIKE REPEAT FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23120">
        <property type="entry name" value="MAESTRO-RELATED HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21047">
        <property type="entry name" value="HEAT_Maestro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23210">
        <property type="entry name" value="HEAT_Maestro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23221">
        <property type="entry name" value="HEAT_MROH2B_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23227">
        <property type="entry name" value="HEAT_MROH2B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MROH2B-like N-terminal HEAT-repeats" evidence="5">
        <location>
            <begin position="46"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="MROH2B-like HEAT-repeats" evidence="4">
        <location>
            <begin position="259"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Maestro-like HEAT-repeats" evidence="3">
        <location>
            <begin position="1018"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="domain" description="Maestro/Maestro-like HEAT-repeats" evidence="6">
        <location>
            <begin position="1419"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1699"/>
            <end position="1738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0407720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1738" mass="191784" checksum="6B4E5162D19F13F1" modified="2022-05-25" version="1">MASSSSAAASAAALEAVQVLVASLADDSPRARDSALAALREIAPLNPLLVLDCCATVSRGGHRRFGNMAGVFLVMASAVRALDRRDAEREFLRKIAKIATAEIVSSKDFNVDWQRAAATLLVAIGSHDPDLMMEEIFLYFSGPTSALPAMLQILADFASAEALQFTPRLKDVLLRVLPILGSVRDGQRPVFANAFKCWCQAAWVYIGDASSGLPFDDDVMSFMNSVFELLLKVWTGSRDLKVRLSSVEALGEMVGLVTRSQLKSALPRIIPTMLDLCKKDQEVAFTAAHSLHNLLSASLLSESGPPLLEFEELTVVLITLLPLVSVNISKDERSYISKGLKTYNELQHCFLVTGLAYPEDLCMFLLSKCKSKDEASIVGALGTIKHLLPRLLESWHTKQTLLVEIVKSLTQEQSLGIRMALAELIVVMASHCYLSGHPAELAVEFLVRHSAITDDDLNDLNTLKNEYFQDKRFEMKISLAGLSELRAVCEKGLLLLAITIPEMELVLWPFLLQLIIPKKYTGAVATVCKCITELCRHKLSQANPLYTEFNASNEMPSPEDLFARLLVLLHNPLARGQLATQILTVLCYLGQLFPRNLSLFWEDEVPKMKAYISDPEDLKQDSTYQEIWDAMIINFLVESLDVVNDNVWVISLGDAFARQYDLYATSDGHSALLHRCLGMLLQKVDDRIYVREKIDWMCRHSSMSIPVNRLGLAQGIGLVAASHLDTVLEKLKNILENAGQSSLQRFLSFFSFREKVEDVDDTYAALALMYGYAARYAPSTVIEARINALVGTDMLGRFLHVQHPTAKQAVITAIDLLGRAVISAAEMGISFPLKRRDQLLEYVLTLMGRDQSDDLVDFSIELLQTQSVALSACTTLVSIEPRLPMETRNRVMKATLGFFALPTEPSNIVESLITNLIILLGAILLTSGEDGRSRAEQLLHILRQLDPYISSSAEHQRRRGCVAVHEVLIKFRNLCSGGFGAMGSYPTLSLKQIDQGGPRGSSSLPSAFVLPNRDSLSLGERIMAYLPRCADTDDEVRKVAIQIIALFFNISLSLPKQKASANDIDLESSYGALSSLEDIVSIIRRNASVDQIEVFHRVVSSVCILLSKDELVVLLHSCTLATCDKVKQSADGSIQAIIVFIIRRGKDLREADVLRTTQSLLSSAVALTDKHSRREVLNAISCLAENTNHIVVFDEVLFVAGRDICTKDITRIRGGWAIQDVFYAFSQHKVLATLFLEYILSILHKEPVATNDSEKGETNSESSADDCILQATMFALNAFLRGGGKVGKQAVEQSYPSVLSALILKLGALHGLAELGRNELLRSLLIAFQSFCECVGDVEMGKILARDGEQTEKEKWIDLVQEVACSSSVKRPNEVLPTCVILSKSLDRNQRAEREAAAAALSEFVRHSEKEPALLEQMVEELCQHVTDDSPTVRSLCLRGLVQIPESHILNYIQQVLGVILALLEDATESVQLTAVQCLLTVLNASEQDAVDPILISLLVRLRNLQISMNTKMRSNAFAAYGALSAYGAGSQHHAFLEQTHATLPRLILHLHDNDLSVRLACRNTFQLLAPLMEVDGLSSLLNKQYFTSDRRSDYEDFIRDLTRQLCRLSPARVDSYLESAIQAFDAPWPVIQANAVCLVSCMLSFLDDQRFLAPYFSQVFAMLVGRMSQSPEAVVRAAASSALGLLIKRSNMLKSLSSRFDRTDPSQSSQHGEPHAKASPELQEEAAAKPNGAQGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNH0</accession>
    <name>A0A8I6YNH0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123402662</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNH0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0625280.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0518840.1">
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        <property type="gene ID" value="HORVU.MOREX.r2.6HG0518840"/>
    </dbReference>
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    </dbReference>
    <dbReference type="OMA" id="DDHQANQ"/>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF395">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PGG"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="759"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0625280.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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    </protein>
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    </gene>
    <organism evidence="11 12">
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        <lineage>
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            <taxon>Triticodae</taxon>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0132">Cell division</keyword>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0690810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="33689" checksum="6F56A049EACB5558" modified="2022-05-25" version="1">MDHTAEHLDPAASASASAPAFVAEVNAWLASLAAEGGGVGGRGGGAVVSELSLGPDPTPRGVSYLRALAVASQARSHAAGIAASGLRAQAVEYQAEAARLREALERAGLARDALPPPAASAARAVAAVANLLAIRDTEMSSFVVASADLWLRRAEVEEKRDKVHKESKALLDYTRKAITKLTELKRMLEKFKNDVEKQQVEQMADWQTKLVMMDSKERQYILQVSNYKAMLNRVGYTPEINHCVLMEMAEHKKDLERKTKPIADTLRSYQDLPPDKALAALAIEDKKRQYTAAEKYLEDVLQSALTTPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YTT1</accession>
    <name>A0A8I6YTT1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YTT1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496490.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0411800.1">
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        <property type="gene ID" value="HORVU.MOREX.r2.5HG0411800"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0496490.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0496490.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0496490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058580">
        <property type="entry name" value="DUF2828"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056690">
        <property type="entry name" value="DUF7788"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011205">
        <property type="entry name" value="UCP015417_vWA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31373">
        <property type="entry name" value="OS06G0652100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31373:SF20">
        <property type="entry name" value="OS09G0499400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11443">
        <property type="entry name" value="DUF2828"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF7788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015417">
        <property type="entry name" value="T31B5_30_vWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2828" evidence="2">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7788" evidence="3">
        <location>
            <begin position="286"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0496490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="432" mass="48191" checksum="DDF554AFF4E1C8D7" modified="2022-05-25" version="1">MHANHPRTLAANLATFARFGCLKDLPEIVYRILHGPRDERKDGDDDSSRVHRRGGSRNNKRRCVGGGAEAAKARRQKETEHAQVVLSRYDSDESFRFLYDSVAELFAELLKSDLEHLRSGDTAKIGLAAKWCPSLRSSYDRSTLLCEAIARRVFPRDSSPEYLGIPDKHYAYRVRNRLRREVHVPLRKVLELPEVYMSAGKWDELPYARVASRAMRQYKLAFDKHDKSGVAGFYDEVRAGLTRIPADAVLPHEILAAALKGEHDESAELQWRRMVSSLVSEGRLSNCIAMCALSSSVEKPPASAAIALGLLISELSQDPWKGRVITFDATHQLHKVRGASLVDKLRTLAAVRAQKGANLQAVFNKILNVAVAGALSKDMMVKRVFVLSDMEFDGWVGGEAWVSEHEAIKKKFAAEGFGVPEVVFWNVGTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YV69</accession>
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        <recommendedName>
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        </recommendedName>
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    </gene>
    <organism evidence="6 7">
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        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
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                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_045102579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YV69"/>
    <dbReference type="SMR" id="A0A8I6YV69"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683800.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0683800.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0683800"/>
    </dbReference>
    <dbReference type="GeneID" id="123409718"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0567670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0567670.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0567670"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0683800.1">
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123409718"/>
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        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Exocyst complex component Exo70"/>
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        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF45">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03081">
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    </dbReference>
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        <location>
            <begin position="216"/>
            <end position="573"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0683800.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="584" mass="64078" checksum="C5F04796C15FD86E" modified="2022-05-25" version="1">MAVSKNAADDMMRVLSIYDARLLPTSPPAEGQATGEAYGCDERDEGEQEQKEEEEAFAAAEDVIRRCNSSSSSSGMIDYLYAVDDALTAAALQGGLASRAAEAVQAAMPRLEEEARALLCSSSARRLSLSSDDLDDAATPPDASPRLSPRAAASVRGVADRMLRAGYGPELAQVYVTARRDALAEAVAHQLGAAEPVAIEEVLRMEWLVLDQKMRRWNHAIRVVVRTLLAGERRLCDEVFASDEELGHECFADVSRGCVLQLLGFADAVAMSARATEKLYRTLGMYEALADVAPELEALFTGDARELFGAEVSAVAARLGSTVRRTIEEFGSAIQGESSRRPVQGGEIHPMNRYVINYCGLLADCRSTLDMILVDTADAVDGDEAPSGGGGATSTPSGRCMLELLTGLLRKMDEKSCLYDDAGLKHIFLMNNLYYIVQKVMDSPLRELLGDDWIRRHRGQIRQYETGYLRASWITVLSFLRDDGGSAAPTVKDKARSFNAAFEELYRNQTAWKVIDPQLREELRIAVSERLIPAYRSFLSRPRPQTGSSSGGSRHSASKHVKYTLEDLEDYMLDFFEGVQKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YVQ6</accession>
    <name>A0A8I6YVQ6_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YVQ6"/>
    <dbReference type="SMR" id="A0A8I6YVQ6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0618880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0618880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0618880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0513280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0513280.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0513280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0618880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0618880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0618880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0618880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="124" mass="13827" checksum="27CAA2B9C58F57E1" modified="2022-05-25" version="1">MEKMKDTPVPEGEEPKSDVEIVEEVLKTKVKQSTFLTNVGLKSSSNNSGKGTADVAAHVRDLEQKLERSALQAEVMQEEMPAIKLKAEEYEAARDKELELLRKKSQEQEEKLAHLMALFGAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YXJ8</accession>
    <name>A0A8I6YXJ8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123407697</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044956822.1">
        <property type="nucleotide sequence ID" value="XM_045100887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YXJ8"/>
    <dbReference type="SMR" id="A0A8I6YXJ8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717400"/>
    </dbReference>
    <dbReference type="GeneID" id="123407697"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0595050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0595050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0595050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0717400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0717400.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0717400"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407697"/>
    <dbReference type="OMA" id="PQWTNIS"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF32">
        <property type="entry name" value="KINASE-LIKE PROTEIN TMKL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035274992" description="Protein kinase domain-containing protein" evidence="11">
        <location>
            <begin position="33"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="374"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="651"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0717400.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1G9</accession>
    <name>A0A8I6Z1G9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123407167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1G9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749430"/>
    </dbReference>
    <dbReference type="GeneID" id="123407167"/>
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        <property type="gene ID" value="HORVU.MOREX.r2.7HG0621770"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0749430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0749430.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0749430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407167"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264">
        <property type="entry name" value="OS07G0554500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0749430.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="406" mass="44590" checksum="0642154F39007A1C" modified="2022-05-25" version="1">MASPPPPCPDAPAAPQAPLPDHLTEDILLRLRTAADLASASVAGPSLRRIVADHSFLRRFRALHPPPLLGILSARFFPAEAPHPSAAAAQTLAGTDFSCSFLPSREQRWRRCDFRDGRALLCVHTDGRAMVRDVAVCDPLHRRYLLLPPVPQDLSDLVNHLDPFLAPAVRDEGADTAADAPFRFRVMCLAKCQTNLVLYVYSSSGPHAGQWRTVTFDGWTALLAGASDHAQSSAAFGKCYYAHGSFCWAICAINKLLLLDTATMEFSSVNLDPDLMNHEVAFVEASEGRLGMFALSQLPIHFVPRLRYSILVTTGDDPGQWQKKEVVTLSHDYCYQIVGVAGGYLLLYVVTDDPDSFPPNKVPDAGCFSLNLRTMDLERFSRGRDIISARNQLYSGFPPYLSPPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1M9</accession>
    <name>A0A8I6Z1M9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Aconitate hydratase</fullName>
            <shortName evidence="10">Aconitase</shortName>
            <ecNumber evidence="3 10">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123410210</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="3 10"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1M9"/>
    <dbReference type="SMR" id="A0A8I6Z1M9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0674600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0674600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0674600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0674600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0674600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0674600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030350">
        <property type="term" value="F:iron-responsive element binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990641">
        <property type="term" value="P:response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="11">
        <location>
            <begin position="10"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="12">
        <location>
            <begin position="631"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0674600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="833" mass="91506" checksum="55194B77A9EC0A69" modified="2022-05-25" version="1">MSWEKTSLEQVEIPFKPSRVILQDFTGVPVLVDLASMRDAMSELGGNPDKINPMVPADLVIDHSVTANVVRSENAIQANMELEFERNRERFAFLKWGSSAFHNMLIIPPGSGIVHQVNLEYLARVVFNRDSLFYPDSVLGTDSHTTMINGLGVVGWGVGGIDAEAAMLGQPMSMVLPGVVGFKLHGTLRNGVTATDLVLTVTQMLRKHGVVGKFVEFYGRGMKELALADRATIANMAPEYGATVGFFPVDHITLEYLKMTGREDETVSTIEAYLRANKMFVDYNEPKIEPTYSSYIELDLGDVEPCISGPKRPHDRVTLKEMKADWHACLENKIGFKGYGVLKDLQDRVVKFNFHGQTAELKHGTVVIAAITSCTNTSNPTVMIASGLVAKKAHELGLEVKPWIKTSLAPGSGVVTKYLLRSGLLKYLSDLGFNLVGYGCTTCIGNSGDLDQIVADAITENDIIAAAVLSGNRNFEGRIHPLTQANYLASPPLVVVYALAGTVDINFEEEPIGTGKGNRPIFLRDIWPSTDEVSEIVHSNVLVDMFKSTYEAITKGNPMWNQLVVPTADVYSWDPNSTYIREPPFFKGMSMAPPGPHSIKDAYCLLSFGDCVTTDHISPAGSIHKDSPAAKYLVGHSVKPGDFNSYGSRRGNYEVMMRGTFGNIRIVNKLLDGEPGPKTIHIPTREKLYVYDAAMRYKTNGQDTIVLAGSEYGTGSSRDWDAKGTMLLGIKAVIAKSFERIHRSNLVGMGVIPLCFKSGEDMDSIGLTGQEQYTIHLPSSVHEMQPGQDIVVTTSTRKSFTCTLRFDTEVELSYFDHAGILQYVMRKLINSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1Q7</accession>
    <name>A0A8I6Z1Q7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123412006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044960897.1">
        <property type="nucleotide sequence ID" value="XM_045104962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1Q7"/>
    <dbReference type="SMR" id="A0A8I6Z1Q7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655650"/>
    </dbReference>
    <dbReference type="GeneID" id="123412006"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0544060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0544060.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0544060"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0655650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0655650.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0655650"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412006"/>
    <dbReference type="OrthoDB" id="643149at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04660">
        <property type="entry name" value="nsLTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044741">
        <property type="entry name" value="NsLTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF46">
        <property type="entry name" value="OS07G0290200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035247581" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0655650.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="103" mass="10461" checksum="5A3F780A2CC168FF" modified="2022-05-25" version="1">MAKAQSMAALLAVVLVLGASADVAHGICNVSSGGIRACQPAAAVRNPTDAPSAGCCAALAGADLSCLCRYKNVGAMWARLYKIDVKRAMALPRKCGLPMPANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z3J2</accession>
    <name>A0A8I6Z3J2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOD1/MUCI70 glycosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123401833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="406" mass="47258" checksum="227A7512E463454C" modified="2022-05-25" version="1">MLERESIRETDAMPRSRKISLNESEPFKRRKKKHYGPCEIEFLPSVDNLVEPADYNNFTQFLLKYILNEGHLNGNGVFEPLFAGHQSLQEREETYYAKNQSLHCGFIEGPEGYPSSGFDLDEHDRAYMATCRVVVSSCIFGGSDYLRRPTKSKIGSYSKKNVCFIMFLDELTLTTLSSEGHIPDENGSVGLWRIVVVKNLPYKDMRRAGKVPKLIAHRLFPSALYSIWLDSKLRLNADPMLIIEYFLWRKKAEYAISVHYDRTCVWEEVLQNKRLNKYNHTAIDEQFYFYQSDGLVKFNASGQDPVLPSYVPEGSFIVRAHTPMSNLFSCLWFNEVNRFTSRDQLSFTYTYLKLRRMNTGRYFQLNMFKDCERRAVAKLFHHRTNGITDPPPTNLHTYKNHSSMSS</sequence>
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                <person name="Schulman A."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <strain evidence="3">cv. Morex</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        </source>
    </evidence>
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        <source>
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                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="1">subsp. vulgare</strain>
        </source>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0026110.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="229" mass="25754" checksum="0F0C9FDF7852DF88" modified="2022-05-25" version="1">MSVCGYRWYPEYTVEEQYRDFNESQHICTVRVFPDYPGAEEPVHWSYGLGVTVDMAVQDAAYSMLTIMRARHALLQNSEFCYVPASQPGEEGYLSGVYIDSSMEDPLLQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTGYGDMEMLNPVRTHLPAHVDWPTIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEVFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B3V1</accession>
    <name>A0A8I7B3V1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B3V1"/>
    <dbReference type="SMR" id="A0A8I7B3V1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0077370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0077370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0077370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0077370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0077370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0077370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0077370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="21059" checksum="755DB1C2EDB9F594" modified="2022-05-25" version="1">MATSLTSSRSRGSCSSPSIPLGRSSPILYREGPLDYQPAIECDYGNKAARWISWSNANPGRRYVKCMRDRFSNRCNLFEFVDDPTPPHLAQLIIDLRDAVLSLKKEKQVVCTEKDDLEMARIADYAEMEPLRRALRKKDQMLMEKDELLLHKTTELHVQKKRNVMLFVVLMCFISVVLYRVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4P5</accession>
    <name>A0A8I7B4P5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proline-rich protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4P5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0105420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0105420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0105420"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0105420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0105420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0105420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33935">
        <property type="entry name" value="OS10G0148100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33935:SF23">
        <property type="entry name" value="PROLINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0105420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="36392" checksum="A1CFD7DCDD427D7A" modified="2022-05-25" version="1">MHSHARRGTYERCDRPPANAKWITKRSSNDDEVDQIVDRCVRWTLDSINRVLIRHPKFRRAKRKLDCLRRPPLFACSQIRSSTSCTVSLNADAYISHAWPVPSINQSIELLATSQASSRVRSTGSGMAAPTRALVLAVLLAMAVANAEAASVVVGLAKCADCTRKNMKAEEAFKGLQVAIKCKNVHGEYESKAVGGLDGTGAFSVPLASDLHGADCVAQLHSAASNAPCAGQEPSKIMPMSEGTTFGIVAGDHTATPSAASPECASMTLCGPIKKHIMEHFHHKKPVPPKPEPKPEPHPDYGPVPKPEPKPQPHPDYHPVPPTPTYGSGGGGGGGGYHGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B5Z8</accession>
    <name>A0A8I7B5Z8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">rRNA N-glycosylase</fullName>
            <ecNumber evidence="1">3.2.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123440406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.</text>
            <dbReference type="EC" id="3.2.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome-inactivating protein family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.22" evidence="1"/>
    <dbReference type="RefSeq" id="XP_044972912.1">
        <property type="nucleotide sequence ID" value="XM_045116977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B5Z8"/>
    <dbReference type="SMR" id="A0A8I7B5Z8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0296770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0296770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0296770"/>
    </dbReference>
    <dbReference type="GeneID" id="123440406"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0247190.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0247190.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0247190"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0296770.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0296770.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0296770"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440406"/>
    <dbReference type="OrthoDB" id="616245at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030598">
        <property type="term" value="F:rRNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.420.10">
        <property type="entry name" value="Ricin (A subunit), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036041">
        <property type="entry name" value="Ribosome-inact_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001574">
        <property type="entry name" value="Ribosome_inactivat_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016138">
        <property type="entry name" value="Ribosome_inactivat_prot_sub1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453:SF47">
        <property type="entry name" value="RRNA N-GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00161">
        <property type="entry name" value="RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56371">
        <property type="entry name" value="Ribosome inactivating proteins (RIP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="1" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035173498" description="rRNA N-glycosylase" evidence="2">
        <location>
            <begin position="35"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0296770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="302" mass="33989" checksum="7D0F89383AE4FE39" modified="2022-05-25" version="1">MSQVAGATGGAFGGAKTKTVFLAMIILFARTAQGQPREIAWDHDAVNAEHTELLDTWHGHVIETVETRFRMKVGDLCDVMAPQRELEDKMTMPPDTWIKPTLKGRGNDKVTLWFSNDDLYFLGYTNLTGHLHTLGGYDGLFLEPFYPLRFGGSYTDLMGVHRTPQGKKIPAHTFVVSVPLGKESLLDAIHVLSSYNPSTPDTDLKIAIAKIVLMGPESLRFRPIRDAYILEWHQETNTYLTLGQTKYLVKWSEISKELVRWEKSGRKIFLKTKGARELKDELGIGSAEEAMGLVYLLLRPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B7B5</accession>
    <name>A0A8I7B7B5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DDB1- and CUL4-associated factor 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123448941</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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        </lineage>
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                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981926.1">
        <property type="nucleotide sequence ID" value="XM_045125991.1"/>
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    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0385670.2">
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53292" checksum="4C2E9258CEB2E8D6" modified="2022-05-25" version="1">MAAVGKASGRRAASCFFEVRKREIGSSSSRASSSQISGSEGLILRMSQYGKLRGHSGCVNTVSFNPAGDLLVSGSDDTDIILWDWLAKTKKLTYPSGHQQNVFHARVMPFTDDSTIVTVAADGQVRVGQMKQGGEFTTKQIGEHHDRAHKMALEPGSPHILYSCGEDGLVQHFDLRSDSPIKLLTCYSFSNRRRRVRLNTIAIDPQNPNYFSIGGSDEYVRLYDFRKINLDSSSNMDLPVDTFCPKHLLMGGKVHVTSIAYSYSSEILVSYNDELIYLFQNYMGLGPNPESTQPEHLDKLEQLQSYSGHRNFRTVKGVSFFGPNNEYVLSGSDCGHVFIWRKKGGKLMRMMRGDKYVVNCIEPHPHFPFLATSGIDKSIKTWTPSSSKVMPLPQYANQIIASNEREREAHASQGEVTLSSDVMMRVLRLQSRRSELYANHEPSAADFATGGDETFFIGLGDGDRNQRSNSDPRECIVT</sequence>
</entry>
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                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    <accession>A0A8I7BC15</accession>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">subsp. vulgare</strain>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0596750.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19179" checksum="6B7C6BF3E636E1B1" modified="2022-05-25" version="1">MARSTRSVATERAYNHFAPSSTRGLMVMGYPATARGPVTADEFDESDIWGSFNPAEEAEQLARSGAELPRVRAVPSARPGRKAKPVDRTGGGGAAHGSLPVAIPDWSKILGDEYHGRQAGDWELDDADDEDIEGAPVVPPHELAWRRRAASLSVNDGMGVGRMLKVRDAVWKKTGFQA</sequence>
</entry>
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    <accession>A0A8I7BE63</accession>
    <name>A0A8I7BE63_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
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                <person name="Fiebig A."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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    </dbReference>
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    </evidence>
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    </evidence>
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</entry>
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                <consortium name="EnsemblPlants"/>
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            <end position="80"/>
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            <begin position="56"/>
            <end position="80"/>
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    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0592590.1.CDS1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="22865" checksum="7C2640DACE7270DC" modified="2022-05-25" version="1">MRYKEMAASKGKPFPFKHVWAILQTFDKWRLRDQETAPKKSAMLRTDDSEDEEEERNLRKPEGTKKGKLRVKMEGEASSLREKMEQMMKAREELTMKTLETKLIITEKKKEIKLAQVEAKHEEAKRKADLEERMIKLKEAKVWKELMVEEKEHMLMSKKDMDQEQLQWWKDYKEDIAERKRIFRDASSTL</sequence>
</entry>
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        <lineage>
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
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    <reference evidence="11" key="2">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_045096510.1"/>
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    <dbReference type="SMR" id="A0A8I7BH45"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598010"/>
    </dbReference>
    <dbReference type="GeneID" id="123402585"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0495720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0495720.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0495720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0598010.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0598010.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0598010"/>
    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
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        <property type="entry name" value="AP2/ERF_dom_sf"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="210"/>
            <end position="267"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="275"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="336"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="435"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
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            <begin position="20"/>
            <end position="50"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0598010.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="457" mass="49117" checksum="332D77BA82BDF8CB" modified="2022-05-25" version="1">MDAADSGGGVRGRERRWKGKAASSAAEKQQQQPLAPVLEDAPAAALLPPLKKMRSPDCRLRRSVSSLSSAPASPDSSSVSNPLSPPATSLPPYASSTRQIFPFAYDPSPAAAPRLLQLLQYSSSLYQQPMLPQQQQHTPSQHPQMISFGDAQQQQQFEAAAALVPPQYMSPEALRYWSAALNLSPRGVLGGVVPPALYQHLLRPPGPAKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEDAAMAYDREAFKLRGENARLNFPDLFLGKGRSGGSGRTSASAAASASSSSSKSAPPTPEETHAQQAQLLLQREQKQHMDEQANVVGPKPLLSAAEQDGLPEPEQNPQLQNAEQQCSDGSTAMMQQAPATPGGVWGPADDAWFSAWGPGSSVWDYDMDSAHGLLLQSRFAGEQAGMDYVPSAPEAHMAPAAGTGTACAAPPSPLPPRPPFMWKD</sequence>
</entry>
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    <accession>A0A8I7BIA6</accession>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
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    <dbReference type="RefSeq" id="XP_044961468.1">
        <property type="nucleotide sequence ID" value="XM_045105533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIA6"/>
    <dbReference type="SMR" id="A0A8I7BIA6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651690"/>
    </dbReference>
    <dbReference type="GeneID" id="123412584"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0540870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0540870.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0540870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0651690.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0651690.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0651690"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123412584"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035161748" description="3-ketoacyl-CoA synthase" evidence="5">
        <location>
            <begin position="16"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="6">
        <location>
            <begin position="28"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="7">
        <location>
            <begin position="333"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0651690.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="462" mass="51438" checksum="0779B8C6361AD854" modified="2022-05-25" version="1">MELISLFAVPVLLEAMSLTYLAWTATSHRRRSQCYLLDYVCYRPPDDRKVTTDMLYEMVERNKYLGMPELRYLFRLTSRAGLGEQTYLPSGVLAGREMCLTHQDALDEMDAFSNDAVAGLFSKTGFGPLDVDVLVVNVNMFNPEPCLASRIAHHYGMREDVAAYNVSGMGCSATVVSLDIVQNIMRARSPRPVLAVVVSTEVLSPGCYGGTDKSMMLGLCLFRCGGVAALLTSDPALDGRAKMKLRRLVRANIAANDDAYSAIFQREDTHNITGYSINKTLPKAAVRAFAANLKRLVPYILPVSELFRLATDLIRQKMSRRQRFKIDVNLKTGVDHFCLHSGGVAVIDAVKKNFGLDEADVEPSRMTLHRWGNTSASSVWYVLAYMEAKGRLKRGDRVLMVTFGSGFKCNSCMWDVTRDLADKGAWADCIDEYPVESTANPYTDKYSWMNDNADDDPSLPVL</sequence>
</entry>
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    <accession>A0A8I7BIP0</accession>
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        <recommendedName>
            <fullName evidence="3">MLLE-like domain-containing protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <lineage>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BIP0"/>
    <dbReference type="SMR" id="A0A8I7BIP0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667480.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0667480"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0667480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0667480.1.CDS1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR056623">
        <property type="entry name" value="MLLE_2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48464:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
            <begin position="88"/>
            <end position="145"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0667480.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="163" mass="18219" checksum="C982E0E0EA394ACF" modified="2022-05-25" version="1">MKEEENEEEDMISIETARQTIDTCSIDTSSSKRQKKEPRTKRAAGPSDPFVSMLQDVNNQLNSVRQHVGIMATPVSAAMARETTQEDPQQKLREKAISELSRLAFTGSEIVEAATIFAKAPEHMNMMLEFPEILRRDFVLKMLSGIIVSKLFLCLSLIIGSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BJ52</accession>
    <name>A0A8I7BJ52_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I7BJ52"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0693280.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0693280.1">
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        <property type="gene ID" value="HORVU.MOREX.r3.7HG0693280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0693280.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="257" mass="29007" checksum="D783E04ED3ABA5D8" modified="2022-05-25" version="1">MPGRPSRQVVRLTRCIDVPGHTAMLVRVMSVCGYRWYPEYTVEEQYRDFNQSQYMCIVRVFPDYPGAEKPIHWSYGLGVTVDMAVQDAAYSMLTIMSARHALLQNSEFFYVPASQSGEEGYLSGVYFDSSMEDPILQSTVEMLENRDRDARALRMELYATRARLWTALTQLAPVVQTVYGEMEMLNPVRTHLPAHVDWPAIGGVTPLRGPLLPPVRGPRPHPCPYGSQGSQARVFPDPQVELPGHGGNLYEMFYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F2CSV4</accession>
    <name>F2CSV4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123424921</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_045108605.1"/>
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        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
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        <location>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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    </protein>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
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                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAJ86459.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK359832">
        <property type="protein sequence ID" value="BAJ91041.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984316.1">
        <property type="nucleotide sequence ID" value="XM_045128381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2CUD8"/>
    <dbReference type="SMR" id="F2CUD8"/>
    <dbReference type="STRING" id="112509.F2CUD8"/>
    <dbReference type="PaxDb" id="4513-MLOC_63766.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0502740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0502740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0502740"/>
    </dbReference>
    <dbReference type="GeneID" id="123451838"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0417600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0417600.1"/>
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        <property type="component" value="Chromosome 5H"/>
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        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
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        <property type="match status" value="2"/>
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        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="LysM_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR33734">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING GPI-ANCHORED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LYSM"/>
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        <location>
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            <end position="41"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAJ86459.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
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<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
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    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
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    <gene>
        <name evidence="4" type="primary">LOC123439474</name>
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    <organism evidence="2">
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Shoot</tissue>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
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        <property type="molecule type" value="mRNA"/>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="SMR" id="F2D670"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0235930.1">
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
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    </evidence>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123398114</name>
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    <organism evidence="1">
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            <taxon>Poaceae</taxon>
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            <taxon>Triticodae</taxon>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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        <property type="entry name" value="OS11G0133900 PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DB49</accession>
    <name>F2DB49_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
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                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Shoot</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLAC1 S-type anion channel family.</text>
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        <property type="protein sequence ID" value="BAJ92320.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    <dbReference type="SMR" id="F2DB49"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273350"/>
    </dbReference>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0226510.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0226510"/>
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    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0273350.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0273350.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0273350"/>
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    <dbReference type="HOGENOM" id="CLU_017679_0_0_1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
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    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09323">
        <property type="entry name" value="TDT_SLAC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030183">
        <property type="entry name" value="SLAC/SLAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269:SF2">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="477"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="503"/>
            <end position="524"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="559"/>
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        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="599"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAJ92320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0273350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="629" mass="69595" checksum="9019881390C1C8AE" modified="2011-05-31" version="1">MELSNKEVQIQMPTQLNERSSREDLPSLLIQVPSRTIAGFDCVGRDAVSSASLHLHEHNNRDTVISIQAPATPPAAAPVPAYDDAQIPYSLSVSMPASPSGLHFSQFRAAAVRRNEARAAVAVAPSDSKPVGQVEVHPPRLLEQTRFHSQPILHASQMNEGTRRADSTRDKRFDPFKTFSGRLERQLSNLRGRPLDPMDLGSHHSKISEETDQVPGTDRYFDALEGPELDTLRATEVAVLPKDEKWPFLLRFPISAFGMVLGVSSQAILWKTLASAPPTAFLHVSLVVAHALWYISLALMGLVSSIYLLKIVFYFEAVRREFYHPIRANFFFAPWIACLFLVQGAPRELAEVHHGVWYMLMAPIFCLELKIYGQWMSGGQRRLSKVANPSNHLSIVGNFVGALLGAKMGIREGPIFFFAIGLAHYTVLFVTLYQRLPTNVTLPKELHPVFFLFVAAPSVASMAWAKINGEFDNGARIAYFIALFLYMSLAVRINFFTGFRFSLAWWAYTFPMTGASIATITYATEVTNVLTRTLSIGLSGIATVTVAGLLVTTMFHAFVLRDLFPNDVSIAITRRKPKFSKILAHFRSSSSDMKELVLSVSKSPNTDSDSSVLGSTDTHPAVTKGKAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F2DC11</accession>
    <name>F2DC11_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123402518</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Shoot</tissue>
            <tissue evidence="17">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family. Matrix metalloproteinases (MMPs) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK361426">
        <property type="protein sequence ID" value="BAJ92632.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK362154">
        <property type="protein sequence ID" value="BAJ93358.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AK363589">
        <property type="protein sequence ID" value="BAJ94792.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044952375.1">
        <property type="nucleotide sequence ID" value="XM_045096440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DC11"/>
    <dbReference type="SMR" id="F2DC11"/>
    <dbReference type="STRING" id="112509.F2DC11"/>
    <dbReference type="MEROPS" id="M10.A05"/>
    <dbReference type="PaxDb" id="4513-MLOC_15950.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0615840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0615840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0615840"/>
    </dbReference>
    <dbReference type="GeneID" id="123402518"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0510650.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0510650.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0510650"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0615840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0615840.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0615840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123402518"/>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015489_4_0_1"/>
    <dbReference type="OMA" id="GPRWPPF"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000018">
        <property type="entry name" value="Metalloendoproteinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF272">
        <property type="entry name" value="METALLOENDOPROTEINASE 5-MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090690" evidence="14">
        <location>
            <begin position="29"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="15">
        <location>
            <begin position="156"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="319"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="12">
        <location>
            <begin position="123"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="11">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAJ92632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAJ93358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0615840.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="369" mass="38143" checksum="C4F28B424861C97A" modified="2011-05-31" version="1">MGKSASALLAAAMAAAAMVFVASSPVAAFPVAGVPGAEPSFPNPWLAFQNLSGCHMGDERDGLARLKDYLSHFGYLSEAPTSSPFSDAFDAELEAAVATYQRNFGLNATGVLDPPTVSQMVAPRCGVADVINGTSTMDRNASAAGHGRHLYTYFPGGPMWPPFRRELRYAITATAATSIDRATLGAVFARAFARWSDATTLRFAEAASESDADITIGFYAGSHGDGEAFDGPLGTLAHAFSPTDGRFHLDAAEAWVADGQGGDAPGAVDLESVAVHEIGHLLGLGHSSVQGAIMYPTIRTGTRKVDLESDDVQGIQSLYGTNPNFKGVAPTSPSTSSREMDSSAGASSRPGGGFAAVVVAVGLLLLLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F2DFD5</accession>
    <name>F2DFD5_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123448964</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK362602">
        <property type="protein sequence ID" value="BAJ93806.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044981958.1">
        <property type="nucleotide sequence ID" value="XM_045126023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DFD5"/>
    <dbReference type="SMR" id="F2DFD5"/>
    <dbReference type="PaxDb" id="4513-MLOC_53110.3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386080"/>
    </dbReference>
    <dbReference type="GeneID" id="123448964"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0321200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0321200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0321200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0386080.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0386080.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0386080"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448964"/>
    <dbReference type="eggNOG" id="ENOG502S2BB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="NPENPMK"/>
    <dbReference type="OrthoDB" id="5837849at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DFD5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044209">
        <property type="entry name" value="MOS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040746">
        <property type="entry name" value="THO1_MOS11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47701">
        <property type="entry name" value="PROTEIN MODIFIER OF SNC1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47701:SF2">
        <property type="entry name" value="PROTEIN MODIFIER OF SNC1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18592">
        <property type="entry name" value="Tho1_MOS11_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO1-MOS11 C-terminal" evidence="2">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="THO1-MOS11 C-terminal" evidence="2">
        <location>
            <begin position="96"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAJ93806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0386080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="174" mass="18053" checksum="E11A12CD853A364B" modified="2011-05-31" version="1">MASQDSKPAQVPAATELTAPAAAAAGEAPNPTSPTAAPNPSSAAAAAAGGAATDLEKKMRRAERFGTQVVMSEDEKRSSRAERFGTGSSNEKMEEQKKKSRAERFGLPTPSSDNTEAKKKARLERFGQSTEVGKAEEEKRKARALRFAGAPSGSSEGKDKDTSKPDAATVAGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F2DLH4</accession>
    <name>F2DLH4_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123394953</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AK364742">
        <property type="protein sequence ID" value="BAJ95945.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044945793.1">
        <property type="nucleotide sequence ID" value="XM_045089858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DLH4"/>
    <dbReference type="SMR" id="F2DLH4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462780"/>
    </dbReference>
    <dbReference type="GeneID" id="123394953"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0383120.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0383120.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0383120"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0462780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0462780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0462780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394953"/>
    <dbReference type="OrthoDB" id="5547497at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="14"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAJ95945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0462780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="332" mass="36322" checksum="8ECC89338A6C5FF0" modified="2011-05-31" version="1">MGSPRKSDKKAALDFAAWSFNITSSVGIIMVNKALMATHGFSFATTLTGLHLLTTTLMTIVFRWLGLSQPSHLPLPDLIKFVIFSNLSIVGMNVSLMWNSVGFYQIAKLCMIPASCLLEVVFDRVHYSRDTKLSIMVVLVGVAVCTVTDVSVNAKGMLAAVIAVWSTAFQQYYVHYLQRKYSLNSFNLLAHTAPAQAGSLLLVGPFVDFLLTGKRVDHFNFTSLSLLFIVLSCIIAIGVNLSQFICIGRFSAVSFQVLGHMKTVLVLFLGFLFFGKEGLNLHVVLGMILAVLGMMWYGNASAKPGGKERRSVLPVRSERHNGGSEDKDGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F2DZ70</accession>
    <name>F2DZ70_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aminomethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123427785</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="10"/>
    <dbReference type="EMBL" id="AK369190">
        <property type="protein sequence ID" value="BAK00392.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044967836.1">
        <property type="nucleotide sequence ID" value="XM_045111901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2DZ70"/>
    <dbReference type="SMR" id="F2DZ70"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194700"/>
    </dbReference>
    <dbReference type="GeneID" id="123427785"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0161520.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0161520.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0161520"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0194700.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0194700.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0194700"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427785"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="OrthoDB" id="10263536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2DZ70">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1250.10:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043303155" description="Aminomethyltransferase" evidence="11">
        <location>
            <begin position="17"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="GCVT N-terminal" evidence="12">
        <location>
            <begin position="46"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="13">
        <location>
            <begin position="330"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAK00392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0194700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="413" mass="44306" checksum="DAE1947212FF6FA1" modified="2011-05-31" version="1">MRGLLACATLARRAAAASSTASSTAARRHLAGAAEVAEAELKKTALYDFHVAHGGKMVPFAGWSMPIQYKDTIMDSTLNCRANGSLFDVSHMCGLSLQGRQAIPFLESLVVADVAALKDGTGSLTVFTNDKGGAIDDSVVTKVNDHHVYLVVNAGCRDKDLAHIGAHMEAFQKKGGDVKWHVHDERSLLALQGPLAAPTLQLLTKEDLSKMYFSDFKMIDINGSACFLTRTGYTGEDGFEISVPSENAVDLAKALLEKSEGKVRLTGLGARDSLRLEAGLCLYGNDMEQHITPVEAGLSWAIGKRRRAEGGFLGAEVILKQLKEGPKIRRVGIFSQGPPPRSHSEIVSGAGENIGEVTSGGFSPCLKKNIAMGYVKNGLHKAGTEFKVVVRGKQYDAVVTKMPFVPTKYYKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F2E0K3</accession>
    <name>F2E0K3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123451234</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK369674">
        <property type="protein sequence ID" value="BAK00875.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984172.1">
        <property type="nucleotide sequence ID" value="XM_045128237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E0K3"/>
    <dbReference type="SMR" id="F2E0K3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090830"/>
    </dbReference>
    <dbReference type="GeneID" id="123451234"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0074740.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0074740.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0074740"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090830"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451234"/>
    <dbReference type="OrthoDB" id="777117at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46700">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46700:SF2">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="139"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK00875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="382" mass="40027" checksum="F20AFDD805810470" modified="2011-05-31" version="1">MGEVAPSGDGDGAAMRRAVRRLSLGAATEDERAEAATEVGRLARSNERIKRALPELGVVLPLVSMLADGGAGAGARTAAAQALLELARGTHRNKVHIVKAGLLKKLPKLMDLSTSQDLALLLLSVSSLANTDFPLSSADLLPFLVATLTATDVPDDTRLACLAALRNLSAKLEHVRAVVTSGAVRALLLPSLLEHTETIAEAALGVLADVAAASAAGRREMAEDEEAPRALVEAMARHEGAGCQEHATYLVMALAHGGGGGRALRWRMRQLGAVQALLEVSLLGSPLARSRAAKILQWFKDDGQDRIRAHSGPRMEAVPSRPCNGKGGGADGTKACRSAVDRIVKQSLDMNMRSIMRRATASVDMTNAKQLVASSSSKSLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2E1W9</accession>
    <name>F2E1W9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123439101</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Shoot and root</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK370140">
        <property type="protein sequence ID" value="BAK01341.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971783.1">
        <property type="nucleotide sequence ID" value="XM_045115848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2E1W9"/>
    <dbReference type="PaxDb" id="4513-MLOC_13071.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218880"/>
    </dbReference>
    <dbReference type="GeneID" id="123439101"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0182230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0182230.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0182230"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218880.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218880"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123439101"/>
    <dbReference type="eggNOG" id="ENOG502R3TU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115622_0_0_1"/>
    <dbReference type="OMA" id="VFERCTR"/>
    <dbReference type="OrthoDB" id="681363at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2E1W9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015090838" evidence="1">
        <location>
            <begin position="25"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAK01341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218880.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="14082" checksum="F0A9FFA28E09547F" modified="2011-05-31" version="1">MAAGGACLLAALAVFSMLIMSSVGNPKPLCSDCGTLCSTNCTAEAKTSCSGYCDYSPREGCERQVFERCTRDGTCCTSNGTCTCDCNTVAQNSCTGVTDNYTDCQACTQGMFDHCYPTCVSDCNNNCKKKGCHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F2EBH8</accession>
    <name>F2EBH8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123451151</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Flower</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373503">
        <property type="protein sequence ID" value="BAK04700.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044984092.1">
        <property type="nucleotide sequence ID" value="XM_045128157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EBH8"/>
    <dbReference type="SMR" id="F2EBH8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0343130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0343130"/>
    </dbReference>
    <dbReference type="GeneID" id="123451151"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0286320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0286320.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0286320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0343130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0343130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0343130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123451151"/>
    <dbReference type="HOGENOM" id="CLU_010119_16_3_1"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000012">
        <property type="entry name" value="Gibberellin 20 oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="192"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAK04700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0343130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="349" mass="39221" checksum="CABA380F81F0A28F" modified="2011-05-31" version="1">MGGLSMGQAFVQAPEHRTKPTLADADGIPVIDLSPLAAGDEAGVDALAAEVGRASRDWGFFVVVRHGVPAETVARALEAQRAFFALPAERKAAVRRDEAAPLGYYESEHTKNVRDWKEVFDFVPREPPPPAAVADGELVFENKWPEDLPGFRVAFEEYAKAMEELAFKLLELIARSLGLTPDRLNGFFKDHQTTFIRLNHYPPCPSPDLALGVGRHKDAGALTVLYQDDVGGLDVRHRSDGEWVRVRPVPDSYVINVGDIIQVWSNDRYESAEHRVSVNSDKERFSMPYFFNPGSDAMVEPLEEMVSDERPARYDAYNWGHFFSTRKNSNFKKLDVENVQIAHFRKLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F2EBL7</accession>
    <name>F2EBL7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123401226</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Flower</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AK373542">
        <property type="protein sequence ID" value="BAK04739.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044950900.1">
        <property type="nucleotide sequence ID" value="XM_045094965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EBL7"/>
    <dbReference type="SMR" id="F2EBL7"/>
    <dbReference type="STRING" id="112509.F2EBL7"/>
    <dbReference type="PaxDb" id="4513-MLOC_57119.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627480"/>
    </dbReference>
    <dbReference type="GeneID" id="123401226"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0520790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0520790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0520790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0627480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0627480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0627480"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006228_2_0_1"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EBL7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RING-H2_WAVH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="CLCR"/>
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        <property type="entry name" value="Vwaint_dom"/>
    </dbReference>
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        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vwaint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="286"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="718"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAK04739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0627480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F2EC18</accession>
    <name>F2EC18_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123426882</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAK04890.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966720.1">
        <property type="nucleotide sequence ID" value="XM_045110785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EC18"/>
    <dbReference type="SMR" id="F2EC18"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0172580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0172580"/>
    </dbReference>
    <dbReference type="GeneID" id="123426882"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0142630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0142630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0142630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0172580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0172580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0172580"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426882"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF33">
        <property type="entry name" value="OS04G0453200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="30"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAK04890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0172580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="514" mass="55378" checksum="F18B20FA85144FD9" modified="2011-05-31" version="1">MARGGLAAAAGSDRVHGYGGGRVTLSVVVTCLMAASCGLIFGYDIGVSGGVTQMESFLEKFFPEVLTGMKGAKRDAYCKYDNQMLTAFTSSLYIAGVLSSLVASRVTRRVGRQAVMLTGGALFLAGSAVNAAAVNIAMLIIGRMLLGFGVGFTAQAAPLYLAETSPAKWRGAFTAAYHVFLVIGTLAATVTNYFTNRIPGWGWRVSLGLAGVPAIVVVVGALLVPDTPSSLVLRGDPDRARAALQRIRGADADVGDEFKDIVVAVEEARRNDEGAFERLRGKGYRHYLVMMVAIPTFFDLTGMIVIAVFSPVLFRTVGFDSQKAILGSVILSLVNLFAVVVSTFVVDRAGRRFLFLAGGVAMMLCQVAVAWILADHLGRNNATTMARNYAKGVLVLMCLYTCSFGMSWGPLKWVVPSEIYPVEIRSAGQAMTVSIALSLSFAQTQVFITLLCAMKYAIFIFYAGWVLVMTVFMAALLPETKGVPLEAMRTVWAKHWYWRRFVGDAKQDSQVNCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>F2ECB6</accession>
    <name>F2ECB6_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123440619</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AK373791">
        <property type="protein sequence ID" value="BAK04988.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044973119.1">
        <property type="nucleotide sequence ID" value="XM_045117184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ECB6"/>
    <dbReference type="SMR" id="F2ECB6"/>
    <dbReference type="PaxDb" id="4513-MLOC_14660.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0306300.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0306300.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0306300"/>
    </dbReference>
    <dbReference type="GeneID" id="123440619"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0255840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0255840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0255840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0306300.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0306300.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0306300"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123440619"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2ECB6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000211">
        <property type="entry name" value="Integrin-linked protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF215">
        <property type="entry name" value="OS01G0892800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="197"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAK04988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0306300.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="476" mass="53505" checksum="30B657739E7764B7" modified="2011-05-31" version="1">MEPGTKVTLQRQASAGSLKQGPSGELRQQTSLESPRSGRAANRYLFGRQSSMDPNRRRGRSQSPVGSQADQDLALPDNLDTTMQLLFLACQGDAHGVEALLQGDVDVNSINLDGRTALHIAACEGHHDVVRVLLDWQANIDARDRWGSTAVADSKCYGHMDIYDLLKSHGAKIPRNKRTPMMVSNPGEIPEYELNPGELQFRKGDEVLKGTYQVAKWNGTKVSVKIVDRETYCDQEAINSFRHELTVFEKVRHPNVVQFIGAVTQNIPMMIVSEYHANADLGSLIQRKGRLHGQKVLRFALDIARGMTYLHQCRPDPIIHCDLKPKNIFLDNGGLMKVGGFGLMRLSKIAPDKVKLMDHEAIVDTFSYYTAPELHRNEVFDMSVDAYAFGFILYEMVEGLPNMESSTVITRCEGMRPSLKGKLKGYPADFKALIEECWETHPMARPTFSEMIVRLDKIYAHCMKQGAWKESLKIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F2ED94</accession>
    <name>F2ED94_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC123436492</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Flower</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="AK374119">
        <property type="protein sequence ID" value="BAK05316.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044971569.1">
        <property type="nucleotide sequence ID" value="XM_045115634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2ED94"/>
    <dbReference type="SMR" id="F2ED94"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053550"/>
    </dbReference>
    <dbReference type="GeneID" id="123436492"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0053550.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0053550.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0053550"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123436492"/>
    <dbReference type="eggNOG" id="KOG2472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2ED94">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000005">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000059">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="305"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAK05316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0053550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="594" mass="66773" checksum="90D632BAEDCCD710" modified="2011-05-31" version="1">MPTVSVGRDRLFAALGRVYTQDEFEALCFDFGIELDDVTTEKAIIRKEKHLEEDVEADGDDEVIYKIEVAANRYDLLCLEGLARSLRIFTGSEATPIFKIASISRGSMLQMHVRPQTSQIRPYVVCAVLRGVTFDEVRYNSFIDLQDKLHQNICRKRTLVAIGTHDLDTLQGPFSYEALSPHEINFIPLKQEQKFRADALMDFYRSDMKLKKFLHIIENSPVYPVIYDSNRTVLSLPPIINGAHSAITLKTRNVFIECTATDLTKANIVLNTMVAMFSEYCENKFEVEPVEVVSYDGSTAIYPDLSCYKMEVSLSDIVGPIGISLDEMQVISLLNKMQLQAELCSSNGEPCISVYVPPTRSDVLHARDLAEDVAIAYGYNNVPKSKPKSMTIGGRQPLNRFSDKIRAEVARAGYMEVLTFVLTSHEENFDMLNRTDDGNKAVIIANPRTSEFEVVRSSLMSCLLKTLKHNIDHPRPIKIFEVGDVVSLDTSRDVGASNNRRLAALYCNSNSGFEEIMGLVDRIVKIVSAPHINFGQTYYVPSSEPEFFTKRQCKIVMSDGKQVGYFGIVHAEVLRKFGIPDPCTFVEIDIEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F2EI89</accession>
    <name>F2EI89_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123411392</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Flower</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AK375866">
        <property type="protein sequence ID" value="BAK07061.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044960268.1">
        <property type="nucleotide sequence ID" value="XM_045104333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EI89"/>
    <dbReference type="SMR" id="F2EI89"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0055780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0055780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0055780"/>
    </dbReference>
    <dbReference type="GeneID" id="123411392"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0043980.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0043980.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0043980"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0055780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0055780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0055780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411392"/>
    <dbReference type="HOGENOM" id="CLU_027462_1_1_1"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF7">
        <property type="entry name" value="EXPANSIN-B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043303167" evidence="5">
        <location>
            <begin position="25"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="60"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="180"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK07061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0055780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="266" mass="28720" checksum="3DC2661FE04C9E1C" modified="2011-05-31" version="1">MGSLSYVLAAAVLASLITGGACIAKVPPGPNITTNYNMNWTPAKATWYGKATGSGPKDNGGACGIKDVNLAPYNGMIACGNIPIFKDGKGCGSCYEVKCNKPPPCSDKPITVFITDKNYEPIAPYHIDLSGKAFGAMATAGNEQTLRSFGELELQFRRVRCKYAPGTKVTFHVETGSNPNYLAILVKFVADDGDIVQMEIQEKSAPTKWIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F2EJS9</accession>
    <name>F2EJS9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123426419</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Plant Physiol." volume="156" first="20" last="28">
            <title>Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.</title>
            <authorList>
                <person name="Matsumoto T."/>
                <person name="Tanaka T."/>
                <person name="Sakai H."/>
                <person name="Amano N."/>
                <person name="Kanamori H."/>
                <person name="Kurita K."/>
                <person name="Kikuta A."/>
                <person name="Kamiya K."/>
                <person name="Yamamoto M."/>
                <person name="Ikawa H."/>
                <person name="Fujii N."/>
                <person name="Hori K."/>
                <person name="Itoh T."/>
                <person name="Sato K."/>
            </authorList>
            <dbReference type="PubMed" id="21415278"/>
            <dbReference type="DOI" id="10.1104/pp.110.171579"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Flower</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AK376406">
        <property type="protein sequence ID" value="BAK07601.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966191.1">
        <property type="nucleotide sequence ID" value="XM_045110256.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044966192.1">
        <property type="nucleotide sequence ID" value="XM_045110257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F2EJS9"/>
    <dbReference type="SMR" id="F2EJS9"/>
    <dbReference type="PaxDb" id="4513-MLOC_11500.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0158900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0158900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0158900"/>
    </dbReference>
    <dbReference type="GeneID" id="123426419"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0130930.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0130930.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0130930"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0158900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0158900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0158900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426419"/>
    <dbReference type="eggNOG" id="KOG4639">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086710_0_1_1"/>
    <dbReference type="OMA" id="MQNYLDK"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="F2EJS9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAK07601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0158900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="152" mass="16935" checksum="CDD20855FA644CE3" modified="2011-05-31" version="1">MVHFKNRYMVMEVFIDASRGEADPVILTQFNITKVIRDSIQLNFGECGLAASLGSLQVKYVNPVTKLCVIRMSREDHQKVWAAITMVRSIGKIPVSFNLLDVSGSIRACKKAALECEEAKFEQYKLAAGDRMTQEIIESVQGCFGKLKGLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-04-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>M0Y3L0</accession>
    <name>M0Y3L0_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123413455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962332.1">
        <property type="nucleotide sequence ID" value="XM_045106397.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962337.1">
        <property type="nucleotide sequence ID" value="XM_045106402.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044962344.1">
        <property type="nucleotide sequence ID" value="XM_045106409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="M0Y3L0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0068950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0068950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0068950"/>
    </dbReference>
    <dbReference type="GeneID" id="123413455"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0055320.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0055320.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0055320"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0068950.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0068950.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0068950"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123413455"/>
    <dbReference type="OrthoDB" id="2012753at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="M0Y3L0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37250">
        <property type="entry name" value="OS05G0496000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37250:SF1">
        <property type="entry name" value="OS05G0496000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0068950.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="123" mass="12785" checksum="A82533164DAECA4A" modified="2013-04-03" version="1">MDGKNLGSGQQAPQAKPDAMTDDQVALKDAAVSDTGNANGHDVVDMNMEAAISADDVMRAGGFGAKDDIGSLLPTAMDSTDFEASLRDARDFEGEKEQPSRPGLGYRATEADAGIKPSDAPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287GNX7</accession>
    <name>A0A287GNX7_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="SMR" id="A0A287GNX7"/>
    <dbReference type="STRING" id="112509.A0A287GNX7"/>
    <dbReference type="PaxDb" id="4513-MLOC_34954.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090630"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0074570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0074570.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0074570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0090630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0090630.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0090630"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287GNX7"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287GNX7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF64">
        <property type="entry name" value="OS12G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="268"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="519"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="6">
        <location>
            <begin position="781"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0090630.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1374" mass="153463" checksum="5CFFAF62B2F62239" modified="2017-11-22" version="1">MEVAVGSAASLLGKVWTTLSDTLVAAYVDSLQLGQNSEEIRDKLLHTQGLLHNAQAQGSHNPGLQGLLEKLSRDADQAEDLLDEVHYFQIHDRLHGTNYATTQDLDGHQALHAGSALRHTLGSWFPCFTCSSSTPKSKKDGGAAAIAGVGGNSGAAAVAGVGGDFAAAAVAGVTNSNSATADDNVDTLLHFDRVSMSREIKSVLQGMQSHCDSVSNLLGTIPSSSTTLILRRPQTASMIIQDTLYGRTDIFEETINRITTVAGATNTQTVSVLPIVGPGGIGKTTFTTHLYNDSRTQDHFQVRVWVCVSTDFDVLKLTREILGCIPATDEEGSSGVPNGTTNLDQLQRSIAHRLKSKRFLIVLDDIWKCDGEDQWKTLLAPFTKGEAKGSMLLVTTRFPKVADMVKTLDPLELRGLESNDFITFFEACIFGEDNKPEKYEDELAGIARKIANKLKGSPLAARTVGRLLYKNLSHKHWNGVLEKHQWLKQQNNDDIMQSLKISYDCLPFDLKKCFSYFGLFPEDYPFTYSEINHFWVAVGIIDINHLANRNYLEELVDNGFLMKKESNYQGGRYYYVMHDLMHELSKSVSAQECLNISDLDFRADAIPQSVRHLSINIEDRYDANFEQEMCKLRKRIDIANLRTLMIFREYEEERITKLLKDTFKEINSLRVLFIRMKSAQDFPYRFSKLIHLQYLKISPPYRSHEMRLPSTLSRFYHLKFLDLGRWLSSFHLPEDFSHLENLHDFHARSEFHSNIRNVGKMKHLQELKEFHVRKESMGFELTELGALTELEGGLVIRGLEHVVTKEEATAAKLMLKRNLEELELLWGRTTDSDILDALRPHPNLRALTIANHGGTVGPRWLCLGDIWLTSLETLTLEGVSWSTLPPFAKLPNLKGLKLKKISGMLQFGPCCGGAPGKCFMRLKEVEFYEMPELAEWVVEPNCCSFPSLEDIRCVDCPNLRVMPLSEVCCTNLRRLEVSGCPKMSLPSMPHTSTLTYLVVKRDTPRRRNFLRADLGTLFYYDGKKLAVRGGYGGALASHNLDKVEDMTVGRCDGLFPEELDGSSVFRSVKSLELDVSHLTSRKSSSSKVLNCFPAVSVLEIVGEGNHDYEKYVMQLPSSSSLQKLSFRGCNGLVLVPAEKENGGGIEEDNSLLQSLTISRCGKLLCLWPSMGMGMGESAETIIYPFPASLRKLYVQEETSIKSMAVLSNLTSLTSLSLSYCRNLTVDGFNPLMAVNLIQLHVQCGKTLAADLLSQVASHSHSQRAELLPAESYISRLEVLSVDDISGLLVAPICNLLAPALHTLEFRSDKRTEGFTEEQDKALQLLTSLHKLTFSCCWGLRSLPQGLHCLSSLKELVSLFVQKSNRCPRRASRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287K811</accession>
    <name>A0A287K811_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123442786</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044974854.1">
        <property type="nucleotide sequence ID" value="XM_045118919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287K811"/>
    <dbReference type="SMR" id="A0A287K811"/>
    <dbReference type="PaxDb" id="4513-MLOC_10356.4"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233160"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233160.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233160.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233160"/>
    </dbReference>
    <dbReference type="GeneID" id="123442786"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0193560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0193560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0193560"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233160.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233160.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233160"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233160.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233160.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233160"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442786"/>
    <dbReference type="eggNOG" id="ENOG502QPZJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="PVGVDHE"/>
    <dbReference type="OrthoDB" id="45251at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287K811">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35512">
        <property type="entry name" value="OS11G0550900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35512:SF1">
        <property type="entry name" value="OS11G0550900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="276" mass="30482" checksum="3B5422B008CAFC5C" modified="2017-11-22" version="1">MLGVSTGQLLVILGACSVMMKPSDMIKMARVAGRMTGRAVGRLMIYRRQMDDIFEQTAAKKINTELQDAMSKLQSIGYEVQNLSRITPGQFIKRQHNTEGMTEAGIYDGSATQSGEFRDDIRSIIRDEIESFCRKNPDQFTRRLNNPDGIQNSEKTTEATKFDVTNKFAMTPKDMESANTSSTNLHSQAMMYARLSVSPGTKMSSPMSTSYGEQFKESNGLLNVLPISAESAGLLPKHTGEPKGSDILLEAVLEAEVAENAKFFVSQPHDPLLPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287K8E8</accession>
    <name>A0A287K8E8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123442795</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044974876.1">
        <property type="nucleotide sequence ID" value="XM_045118941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287K8E8"/>
    <dbReference type="SMR" id="A0A287K8E8"/>
    <dbReference type="FunCoup" id="A0A287K8E8">
        <property type="interactions" value="1938"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287K8E8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233670"/>
    </dbReference>
    <dbReference type="GeneID" id="123442795"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0233670.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0233670.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0233670"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287K8E8"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287K8E8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000012">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000010">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="270"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="427"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0233670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="464" mass="52372" checksum="970A2DB6625715BF" modified="2017-11-22" version="1">MAAGDGGDTVAVPTEIAALRLRVLELERENQRLAQIASTCSCGSKEDNIPPSLVENRDQQVQKNVKSQKSRISDYDVSLPFDHMMISTENQVLCDVKGEGHDDLSKIRRKHSKRTGHQVGNVSHCQKRLIALKIMYFGQRFYGFASEAHTEPTVESEIFKALERARLLVGSIKDACYSRCGRTDKGVSATGQVISLYLRSNFKEAGGNVLGERSEIDYVSVLNRNLPRDIRVIGWCPVSADFLARFSCLGREYKYLFWKGGLDVSKMQEAAFKFIGEHDLRNFCKMDAANVSNYKRCITDFTISACDQRSNHDELWSMNIRGSAFLWHQVRCMVAVLFLVGQGLESPCVVDSLLDITKTPRKPQYTMAPELPLILRSCLFDRVNFMCSSDASQALIEHLKDEYHQYMLQAAIFDEALTCLCIPEANPSESPKKKRKHIPLLSRETEPSYEERRARVKAKSATCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287PD19</accession>
    <name>A0A287PD19_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123449185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044982235.1">
        <property type="nucleotide sequence ID" value="XM_045126300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287PD19"/>
    <dbReference type="SMR" id="A0A287PD19"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392940"/>
    </dbReference>
    <dbReference type="GeneID" id="123449185"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0326890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0326890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0326890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0392940.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0392940.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0392940"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449185"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010476">
        <property type="term" value="P:gibberellin mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000215">
        <property type="entry name" value="Os03g0241600 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000268">
        <property type="entry name" value="probable receptor-like serine/threonine-protein kinase At5g57670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000284">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046958">
        <property type="entry name" value="RBK1/2/STUNTED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987">
        <property type="entry name" value="OS08G0249100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF11">
        <property type="entry name" value="RECEPTOR-LIKE CYTOSOLIC SERINE_THREONINE-PROTEIN KINASE RBK1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0392940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="663" mass="71646" checksum="9C2CD3C5B490EB08" modified="2017-11-22" version="1">MAMAGEGVGRCILVGLHMDAVGKELLKWALNQAARSGDRVVAVHIYRKSGDLCKTNALTLIRTLDEYLAEYEAICSKKDIVLIGRVTPGSSIQKVLVKEAKLCAAMVVVIGTNKKYSFGGSTCLAKYCAKKLPPTTSVVAIQGGKAIFVREAPRPPLGAEPKPVLRTLLHPSVGMEPKVIIPNPNRRSARSMDLDAVGCGQCAAPQLTKPCDDDGNTADADADAKAVVVRAPAAPEQRLGWPLLRRAPAASTATAREHEATRKQSVVHWVMSLPRRSSPSASPEPAQEGLAAELRRTLGGVPSRCRWFRYEELYDATNHFSPGNLVGKGAHSRVYRGGLASGQRVAIKLCRASAEASKDFLREVDIITKLQHGRIVPLVGVCVEGPNLISVYRYLPRGSLEDNLHGKRSKPALPWEKRYRAAVGVAEALSYVHSGGSRPVIHRDVKSSNILLTDGFEPQLSDFGLAIWAPSSPSSLTHSDVVGTFGYLAPEYFMYGKVTDKVDVYAFGVVLLELLTGRRPITGDGSPKGHHQSLVMWATPILNGGDISDLLDPSLDVKHDEVEVRRMAVAASLCLGRSARLRPPISQILSILRGEEDATSLAASEPDCVVDDETYPAANVRSHLGLALLDVEDAESISSTEHTNLSPLEEYLRQRCSRSSSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287R6L1</accession>
    <name>A0A287R6L1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A287R6L1"/>
    <dbReference type="SMR" id="A0A287R6L1"/>
    <dbReference type="FunCoup" id="A0A287R6L1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287R6L1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0387890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0387890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0387890"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0468570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0468570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0468570"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A287R6L1"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287R6L1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044517">
        <property type="entry name" value="PHOX1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46183:SF3">
        <property type="entry name" value="OCTICOSAPEPTIDE_PHOX_BEM1P (PB1) DOMAIN-CONTAINING PROTEIN _ TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46183">
        <property type="entry name" value="PROTEIN CLMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0468570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="938" mass="106367" checksum="62E1448B2C784688" modified="2017-11-22" version="1">MEEVPSEKKQTESESINCATSSSSNQDIPNSLVNKDEDPAVGVVTELKKEGTSVGQKEESASTAIDLKEEGTATQLKEEGAATQLKEEGASKEDGAAIDSKEEGAKTELEEKLMLSQRKDYEKAIELKGEGTRLFQRRDYEGAALEFDKAIKLLPKEHNDIAFLHCNIAACYMHMNPESYERAVDECNAALEASPKYTNALLKRARCFEALDRLDLACEDVQKVLSLEPNNVSALELYESIREEMETEKLLENQVVSPVEPKAGFSKEKIQRKISRKFRNNSIVEEVWLIHDDDAQEDDEDDTDEECSEENHVENDLSNEGNDTRDMPSRHNHDEDKRNTEQNHVKHGQYKLGASDEGQQLQHASRDMEQIHCKETESQNKHENPLKEIKVRSGQHQQNQVGDMDMRQKQIEDVPTTSRSKQEINTEMYERFINGGQARRSLQRHTSRGGEKQEKQTPIKLASHGGDEHQKQKRTYATNGDTKTVKFVMGDDIRIALVPEECSLLQAISVARCKYSPHLKAMLLKFQDIEGDLVTITSTEELRWVEDLKQGPARLYIKEVSPEREITKDIVMPNISTVMLERKHSVSECESSRHAAEDKSSSYADDWMVQFARLFKNHVGFDSDALVDLGDLGTRLCIEAMEDTITSEEAQEIFHAAEAKFQEVAALAFFNWGNIHMSRAKKRLSLSEDASKESVLSQVKTAFELACAEYAKAGKKFEDTVDVKPDFYEGLIALGNQQFEQAKLSWRYADACKVDMGTEVLELFNRAEDNMEKGMEMWEGIEYLRLKGLSKSKKEKILLDKLGMDGHQKDLTADDAFEQASNMRSQLNISWATILYERSVVEFKLGLASWEESLTEAIEKFKTGGASLADISVMIKNHCANEKTQEGLSFKIDEIVQAWNEMYDAKKLKNGSSSFRLEPLFRRQPSKLHNVLEHIKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-11-22" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A287XIX1</accession>
    <name>A0A287XIX1_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123410378</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959253.1">
        <property type="nucleotide sequence ID" value="XM_045103318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A287XIX1"/>
    <dbReference type="SMR" id="A0A287XIX1"/>
    <dbReference type="FunCoup" id="A0A287XIX1">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="112509.A0A287XIX1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0729620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0729620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0729620"/>
    </dbReference>
    <dbReference type="GeneID" id="123410378"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0605300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0605300.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0605300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0729620.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0729620.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0729620"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123410378"/>
    <dbReference type="InParanoid" id="A0A287XIX1"/>
    <dbReference type="OMA" id="MPEAVED"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A287XIX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000423">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046958">
        <property type="entry name" value="RBK1/2/STUNTED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF30">
        <property type="entry name" value="OS06G0693200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987">
        <property type="entry name" value="OS08G0249100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0729620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="386" mass="42312" checksum="4DEB44F1D141F7BE" modified="2017-11-22" version="1">MKPLYLRSSGSFKKLLLSLSPHRAKRVHAPPDAAEESESAPQERKPEWECFSYEEVRRATDGFRAGNLVGTGGSSEVYRGELADGRAVAVKRLLGASACERRERDFLAELGTVGHARHPNVCPLLGCCVDRDLYLVFAFSARGSVSANLHDDGEGAPAMGWEARYGVAVGTARGLEYLHKGCRRRIIHRDIKASNVLLTDDFQPQISDFGLAKWLPTEWTHRAIAPIEGTFGCLAPEYYTRGIVDEKTDVFAFGVFLLEVMAGRKPVDGTHRSLLSWARPLLSEGKTEALVDPRIVAGGGYDAERARRLAFVASLCIRASPTWRPSMTEVLELLEGVEAIKEERWAMPEPEAGDDEGEEVWGFDDLDDDEESGTPSPSSTSSASSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-06-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2R9IVW3</accession>
    <name>A0A2R9IVW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123445663</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978616.1">
        <property type="nucleotide sequence ID" value="XM_045122681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2R9IVW3"/>
    <dbReference type="SMR" id="A0A2R9IVW3"/>
    <dbReference type="PaxDb" id="4513-MLOC_11938.1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0327630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0327630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0327630"/>
    </dbReference>
    <dbReference type="GeneID" id="123445663"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0273090.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0273090.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0273090"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0327630.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0327630.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0327630"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445663"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2R9IVW3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20506">
        <property type="entry name" value="CYCLIN_AtCycA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20562">
        <property type="entry name" value="CYCLIN_AtCycA_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000013">
        <property type="entry name" value="Cyclin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000167">
        <property type="entry name" value="Mitotic cyclin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="266"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="359"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="363"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0327630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="56643" checksum="5A5B7D5BEF6BD145" modified="2018-06-20" version="1">MAARKENAVLIACQAPNGRVTRAQAATNRGRFGASLPAQVPVRTERKPATKGKAKRGASDDNTCASALISAPPPKRRAALKDVTNISCANSSRNSTAVTKHQARPTQKAGRNPSKNKQCMKKVPKPPLPAVSGTSFVVNDSHNVEEAQKAELLPPKEEPTALLENKGSFSLWNIARNRESGVHEPFFQGRNTRDKSETADSNTGDYVGLNVIDIDKDNGNPQMCASYVAEIYRNLMAAELIRRPKSNYMETLQRDITKGMRGILIDWLVEVSEEYKLVPDTLYLTVYLIDQFLSRKYIERQKLQLLGITSMLIASKYEEICAPRVEEFCFITDNTYTKNQVLRMECEVLNDLGFHLSVPTIKTFLRRFLRAAHASQKAPCATLGYLGNYLAELTLTDYSFLKFHPSVVAASAVFLAKWTLDQSDLPWNCTLEHYTSYKSSNIERCVRDLWELQQGNTSGSPPLNAIREKYRHEKFECVANMLSPAMPVSLFRRQANDTTPLLINDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WDH5</accession>
    <name>A0A8I6WDH5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 5">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044953447.1">
        <property type="nucleotide sequence ID" value="XM_045097512.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044963036.1">
        <property type="nucleotide sequence ID" value="XM_045107101.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044963038.1">
        <property type="nucleotide sequence ID" value="XM_045107103.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044963039.1">
        <property type="nucleotide sequence ID" value="XM_045107104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WDH5"/>
    <dbReference type="SMR" id="A0A8I6WDH5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001570"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0769180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769180"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0769200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769200"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0769210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769210"/>
    </dbReference>
    <dbReference type="GeneID" id="123403585"/>
    <dbReference type="GeneID" id="123418979"/>
    <dbReference type="GeneID" id="123418980"/>
    <dbReference type="GeneID" id="123418981"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0001570.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0001570.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0001570"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0769180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769180"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0769200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.UnG0769210.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.UnG0769210.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.UnG0769210"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403585"/>
    <dbReference type="KEGG" id="hvg:123418979"/>
    <dbReference type="KEGG" id="hvg:123418980"/>
    <dbReference type="KEGG" id="hvg:123418981"/>
    <dbReference type="OrthoDB" id="581410at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="2">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Jacalin-type lectin" evidence="2">
        <location>
            <begin position="152"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0001570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.UnG0769180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="302" mass="32100" checksum="058C2BB05DBB784F" modified="2022-05-25" version="1">MAVVMKVGAWGAPDGSPQDINAESRPQRLESITIYSVESPGVCGIKGFSFKYVDQQGSSVKSAIWGSNSGNPNTIEMREGEQLKLVGGTFDNEGIGSLTLETNTTKHKTYGYPVQGGEFSLPLPQGKGELVAFFGRSDVTLKALGVYVKGSPAKVGKWGAKSGAPRDIRPDADPCKLESFTIHSSERIHGFSFTYLTKSDQAISVPLWGKKAGEEHTIFMNQSEYVSSITGAYDTYGITFLNFDTNQGASHTFGRNPSAGTKTFSVPLPDNGALDDAAAVAFFGSSGDRLVAIGTYVGVAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WJ60</accession>
    <name>A0A8I6WJ60_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WJ60"/>
    <dbReference type="SMR" id="A0A8I6WJ60"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0089330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0089330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0089330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0089330.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0089330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0089330.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="255" mass="28565" checksum="B3F8B465338364F3" modified="2022-05-25" version="1">MVSYDEIIVNYMQIVPSSVSGKKFSSYLDACMGEPVVTEMDPSETPGHQKIQSYLVQSPDNSGEEVEVIPTPRVREEMQVRFSKRNMEANVEHVGVRVDRMAQKRDLQGNRISPGNSFEVLSNFEIVSAAAKMGVNIPDDTFETIDIIRELEISTANIAKKVDKNDKEQDKILFITNAVGEASPLDTEWVGDEGLDVDYFTMVRSRKKERKKVNITISKPVTRSQKQKVGDGAGKAMAPRKPSTKTQLPKNRKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WKX6</accession>
    <name>A0A8I6WKX6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
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                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
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            <dbReference type="DOI" id="10.1038/nature11543"/>
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    </evidence>
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        </source>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
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    </reference>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6WTJ9"/>
    <dbReference type="SMR" id="A0A8I6WTJ9"/>
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            <end position="141"/>
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        <source>
            <dbReference type="RuleBase" id="RU367018"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0179810.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="141" mass="15203" checksum="1E7A61490605672B" modified="2022-05-25" version="1">MLCCHILKVFNQLGVDEIPAQYIFRRWTPIAISDAPPAVEDKPDELPAQTKKELRHANLVMDFGSIARVASASDAATDIVKKHMRGARHEIRNLNMSRKQKPAVPPAGPTGGPQASAGGSTPPPSNTRAKRGHVLDVDGPK</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
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        <lineage>
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    <reference evidence="3" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
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                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    <dbReference type="OrthoDB" id="671172at2759"/>
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        <property type="component" value="Chromosome 2H"/>
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        <property type="entry name" value="AT3G44326-like"/>
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        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0166680.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="31946" checksum="D9523260C6A8DB1E" modified="2022-05-25" version="1">MVSTIEDLHADVLACALRRLDGRSLAAASCATAGLRALAADPGTWRALCLAEWPSLAAQQHRHGLLSAVPPRRLFADASPFPCPDAGELGAGVLPAKLVSAVDVYYRGAPVLSRVVETPASSPWFLSAPFRVEAVDCKKPAPVAAAFSPAELELSWVVVDPARGRAVNVSSRRAVALGRHWYTGEMLVRFAVVLGGCNFETTVACSEEAGHVSEVSLAVENADGAAVSGEGALRLLAAAMEARRRGGEREREEAKARYEVFVRNKKGRKESKARREVLVDLCCSAASAVAVLAFFATVVLR</sequence>
</entry>
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    <accession>A0A8I6WVJ7</accession>
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        <recommendedName>
            <fullName evidence="8">Protein argonaute 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123427598</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_045111674.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8I6WVJ7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0190470.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123427598"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0157800.1"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
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    </dbReference>
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        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="PAZ" evidence="4">
        <location>
            <begin position="401"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="5">
        <location>
            <begin position="703"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0190470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1047" mass="113040" checksum="ABD54C092C3FB066" modified="2022-05-25" version="1">MDYEQGGGGGRGRGRSRGGGGRGGAPGGYGPQGGGGGYGGGGQGRGAQGSGGGYGGGGGGGYGPQGGLEGRGGGYAPRGGGYAPRAGRGYGPQGGGEGRGGGYGGGGPGGRGPGGGGRGYGPGGGRGGNAWAQPGRGPAGGPGDYAPVRAPAPAPASAARRVGSEEAGGSSGSVERISSKEVAKLEPLAPPVAASPNGIRVPMRRPDDGGSLCQARVQLLVNHFIVKYPKLSTFFHYDIDIKFDPASSKVSGKELSNADFLSAKAELFKDDSFRQLSSAVAYDGKRNLFTAAQLPEGLFRVRVHSKTYIVSVEFKKQLPLSQISELPVAREILQGLDVIVREASTWRKIILGHGFYSPDSKVDMGSGVVSMKGTQQTLKHTQQGLVLCVDYSVMPFRKDGPVLDIVRQFIKPLPLDYRTALNKTHREKLVYELKGQRITVSHRKTKQKYTIQGFTDLPASQITFLDSESGQTKRLVDYFSQQYGKVIEYQMLPCLDLSKSRDKPNYVPIELCKLVEGQRYPMANLNKDTERALKGKALIKAAERKWEIETAVKAEDGPCRGEIAQQFGISLDVKMMEVTGRVLTPPSLTLGSSRGGPGNISITPSNCQWNLMGKKLVEGKALQCWGIVDFSARPSHNKQQPLDGNMFINYIVRKCCDLGIQMNKTACFVHLSEMSVLSDPHQLHEELNKAKQAAVKKNQKLQLLFCPMSEQHHGYKTLKLICETQLGIQTQCFLSHLANKTQGQDQYMSNLALKINGKLGGINTQLQDKLPLDNGVPYMFIGADVNHPSPGNGESPSIAAVVASMNRGATKYVPRIRAQPHRCEVIKNLGEIVQELIGVFEKKAGVKPQRIIYFRDGVSDGQFEMVLNEELADMENVIKVKGYSPTITVIVAKKRHHTRLFPKEHNEPLQTKNGNVLPGTVVDTRIVDPVTYDFYLCSHNGLIGTSRPTHYYNLMDEHGYGSDDLQRLVYNLCFVFARCTKPVSLATPVYYADLAAYRGRLYYEGMLASQPQVRSSSSSASSTAAGACDFSNFPTLHVDLQDNMFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WVK1</accession>
    <name>A0A8I6WVK1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WVK1"/>
    <dbReference type="SMR" id="A0A8I6WVK1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073910"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0073910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0073910.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0073910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF17">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="54"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0073910.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="233" mass="26088" checksum="50382EEB1A1C75E6" modified="2022-05-25" version="1">MEPPEDVVVSNLFEHQKAALGWLVHREESCDLPPFWKEDKAGGYENVLTSQNTKQRPQPLRGGIFADDMGLGKTLTLLSLIARSKARNVGGGKAKGSKRRKIDDAEAGWRTTLVVCPPSVFSSWVTQLEDHTNARSLKVYMYHGQRTKDKEVLLKYDIVITTYSVLGTEFDQEGSPVNDIEWFRVILDEAHIIKNSAALRTRAMTALKALGSHRDANSEQLIEFVPAYGVFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WXA2</accession>
    <name>A0A8I6WXA2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAHD acyltransferase DCR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123446913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044979397.1">
        <property type="nucleotide sequence ID" value="XM_045123462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WXA2"/>
    <dbReference type="SMR" id="A0A8I6WXA2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076820"/>
    </dbReference>
    <dbReference type="GeneID" id="123446913"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0062280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0062280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0062280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076820.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076820.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076820"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123446913"/>
    <dbReference type="OMA" id="WGKPESV"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010090">
        <property type="term" value="P:trichome morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF76">
        <property type="entry name" value="BAHD ACYLTRANSFERASE DCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="47786" checksum="FC304F5BE5A4C7BA" modified="2022-05-25" version="1">MAVDNGDARAAAMAVAVTGTRTVAPAKTKVTLATFDLPYITFYYNQKLLLYRLAEGAGDFQDVTARMADALGDALAYFYPLAGRIRQEKGDGGALYVDGEEGAEVLEAAAEGVSVDELAGEDCGEEAEKLMQQLIPYTGVMNLEGLHRPLLAVQFTKLKDGLAVGCAFNHAVLDGTATWHFMSSWAELCRGAAAPSAIPIHNRAMARSVRVGLTLPASAEEHEKTDPNGPKKPLVARVFSFPEPVVARIKAAANAALPAGAKPFSSFQSLGAHIWRSVSRARGLGPADITVFAVFADCRARLDPPVPATYFGNLIQAVFTGVPAGMLLGGPPELAAGMLQKAIGEHDAAAVTRRLEEYEAAPKLFHYSDAGPNCVAVGSSPRFRVYDVDFGFGRPERVRSGGNNKFDGMVYLYPGRGGDSGGIDVELALQPEPMQRLEKDDEFLQVAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6WY45</accession>
    <name>A0A8I6WY45_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123448396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981188.1">
        <property type="nucleotide sequence ID" value="XM_045125253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6WY45"/>
    <dbReference type="SMR" id="A0A8I6WY45"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0081100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0081100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0081100"/>
    </dbReference>
    <dbReference type="GeneID" id="123448396"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0066400.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0066400.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0066400"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0081100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0081100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0081100"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448396"/>
    <dbReference type="OrthoDB" id="1911459at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34970">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34970:SF3">
        <property type="entry name" value="OS05G0535700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0081100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="80" mass="8537" checksum="2124E1D9892B0A7C" modified="2022-05-25" version="1">MGFMLRVRLASFFAGAAAAAAAGGYFLYKDYKLAHDSTALQVKGLHDRVDARYKAMDKRLAALEAQKSADSSPGVPTPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X152</accession>
    <name>A0A8I6X152_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial ATPase complex subunit ATP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123426897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044966738.1">
        <property type="nucleotide sequence ID" value="XM_045110803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X152"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027680"/>
    </dbReference>
    <dbReference type="GeneID" id="123426897"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0021840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0021840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0021840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0027680.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0027680.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0027680"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123426897"/>
    <dbReference type="OrthoDB" id="17089at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007849">
        <property type="entry name" value="ATP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05176">
        <property type="entry name" value="ATP-synt_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0027680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29549" checksum="154EB94D3005E85A" modified="2022-05-25" version="1">MMLRARRAAGPLLRLADAGAVGERICGGAQPARAVFARGFLDFLKPWSKESPENAEKKAKARARLTDEMSRGYFQDISEIRKNNGKIAAANKIIIPEAAAVKFPNLALESPAGRALQLPLVASPPLGDSHGADGTVPVVPVAALVCLSFRASSQKMAESWSLPFLDAFGATRKVEAYEVSFIDSWLLSSSPLRRAFLKAMRKSNNPQRHAVYAFGDHYDFRKELQIVNLLTGYIYLVDRLGRIRWQGFGAATKEELSSLTACASILLDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X1P8</accession>
    <name>A0A8I6X1P8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123428230</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 13">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044968358.1">
        <property type="nucleotide sequence ID" value="XM_045112423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X1P8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207170"/>
    </dbReference>
    <dbReference type="GeneID" id="123428230"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0172200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0172200.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0172200"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0207170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0207170.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0207170"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123428230"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="UniPathway" id="UPA00845"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045489">
        <property type="term" value="P:pectin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000056">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF74">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25557">
        <property type="entry name" value="GAUT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0207170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="570" mass="65044" checksum="F3792F1637421DAD" modified="2022-05-25" version="1">MVRPAGLLDPTTRRRPFLDQRRPSFRRRWQQRPLWARLLLSLLLALACALLLLGLAGSPDPSSPPATDDPSRSAHADADAVSSPLLRQRSYLEVITDTHNMTDEMLSAHSLSRQLMDQISLAKTYITVAKEANNLQFVAELGSLVRREQSILARAAANGGTVVKEDAEKAIRDMAVLFYQAQQFRYDSAVTILKLKGQILSLEEKSKAEADKSTKYGQIAAEELPKGLYCLGVRLTMEWFNSAELQRKFLDRSPAVQSNLRDNSLYHYCVFSDNIIAVSVVVNSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDYRGAIVEIQKVEDFTWLNASYVPVLKQLQDAATQNFYFSGSGNRGTPIKFRNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETCMETFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIYHYWQERNADHTLWKLGSLPPGLLAFYGLVEALNPKWHVLGLGYTTVDPDAIKEGAVLHYNGNLKPWLKIGMEKYKGFWDNYVDYSHPLLQRCFMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X2Z0</accession>
    <name>A0A8I6X2Z0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VDE lipocalin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123444277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044976890.1">
        <property type="nucleotide sequence ID" value="XM_045120955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X2Z0"/>
    <dbReference type="SMR" id="A0A8I6X2Z0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285060.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0285060.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0285060"/>
    </dbReference>
    <dbReference type="GeneID" id="123444277"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0236500.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0236500.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0285060.1">
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    </dbReference>
    <dbReference type="KEGG" id="hvg:123444277"/>
    <dbReference type="OrthoDB" id="420426at2759"/>
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        <property type="component" value="Chromosome 3H"/>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:violaxanthin de-epoxidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:xanthophyll cycle"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calycin"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33970:SF2">
        <property type="entry name" value="OS01G0716400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="246"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0285060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="528" mass="58503" checksum="F917255DA9540DD0" modified="2022-05-25" version="1">MATTLPTAAAAAAAATSTVRIFQPTRFHLPRTSPVLAPSRSASRRLSGITCSPGIASVAGKATSPEDAVRIVAVVGDGSISPLKDTPWDEVMRHTADRLKWVDEGFEMLVFTDKSIDHDDLRKELSHCDMLVNVAITGQESVQWLTQNSKNIPNVLCFQSSPSLVNKLGGTSVQYTGEQDFFGKLINVGKPSGTEESTEVLKTISNAWERHNSDDIRFCLLVVVNAYIRPVAMLKNLRAKGLSTLGCMIKNCGPQILNCLFDPDCRKAIQCLNSCSPTDQVCNYRCIASYESPHLEAFSLCVLQKNNCLELNAEIPSKPSVTPLSVFREEKLSHEVAEDLFVGWLDSLEWSWRVVAGQNPAYDQFPCQYQLFYRGKAKGSFWYEPIFQVRTLEGKLVWRRRKYRVKRASIPGTFYFSVLDNGVVSKEFWTVVDVSDDFSWGLFHYHGAAQAAGQSYTGAVLVTPDGSYPDGDNPRLDSALEKCGIKKWELFMVDNCSCTGAPLGTPEGSRLHYQIAPGKEADIMQRIL</sequence>
</entry>
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    <accession>A0A8I6X352</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
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    <dbReference type="RefSeq" id="XP_044965424.1">
        <property type="nucleotide sequence ID" value="XM_045109489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X352"/>
    <dbReference type="SMR" id="A0A8I6X352"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0023200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0023200.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="123425761"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0023200.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0023200.1"/>
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    <dbReference type="KEGG" id="hvg:123425761"/>
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        <property type="entry name" value="Tubby-like_C"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tubby C-terminal domain-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0023200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="219" mass="24331" checksum="913EFA57A39D2212" modified="2022-05-25" version="1">MVMAQAEPEETPRSQQRPSAGGGPVAGEPSQPAPATPRRLTVWCKSLVFGGDGYAVFDDADGRMVFRVDNYGVGPGSMALMDHAGRVLLTVRRRQRKMLSLMPETWEVYNGDVDDDGHGERCLMIRATKDVCSSSCTASVLAVDEPERYLMRWSRREEWSRIYRRSSTGNETLIAEVRRKRGGPEKATLLGKDVLSLVVQPGMDLAITMSMLMISNSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X5D9</accession>
    <name>A0A8I6X5D9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123445131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044978006.1">
        <property type="nucleotide sequence ID" value="XM_045122071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X5D9"/>
    <dbReference type="SMR" id="A0A8I6X5D9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307410.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307410.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307410"/>
    </dbReference>
    <dbReference type="GeneID" id="123445131"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0256840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0256840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0256840"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0307410.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0307410.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0307410"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123445131"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000005">
        <property type="entry name" value="DNA repair metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000039">
        <property type="entry name" value="DNA repair metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF31">
        <property type="entry name" value="DNA REPAIR METALLO-BETA-LACTAMASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="228"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="615"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="623"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0307410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="635" mass="70255" checksum="6EE418A6372BF423" modified="2022-05-25" version="1">MPIEMPRGLPFAVDTWGPSSRPRRHRFLTHAHRDHLVGAAGAGPGGGGAVYATRLTLSLALRHFPQLDQGEFVEVEAGRTIEVDDPAGAFSVTAYDANHCPGAVMFLFEGEFGSILHTGDCRLTPDCAQNLPLKYIAKKGKENVCRLDFVFLDCTFSKCFLKLPSKELAIQQVIACIWKHPDAPFVYLACDLLGHEEILVEVSRTFGSKIYVDVRRNSDCFKALSLTAPEIITDDPSCRFQIVGFHHLYDNASKKLEGARASLQPEPLFIRPSTQWYACGRNQKPSLTEAQQDDFGIWHVCFSIHSSRDELEQAMQLLQPQWVISTTPPCFAMELSYVKKCCFKTRLTTDDPLWKIFRDPLQKSVSSPSSVLASCTPPDEDPSTFVEDDHPTSVSEECTDFDVSTLELQFVPSPPVEEPDITLFGRARFGSQVIDIMKEELCHQYIAAAEQTKLCVPEDLPHDSSRNVETYTGMDLITKPAPASQQDCVEAGDVSPSCQCAEARDVPPSCQCKASPTLPVPEAFAVQPLPTVQHNISVAPDQPEKPVTIIEPISMGTTESSSLKNVEITDCQMDRLCVIGSSKSLHASLKRLYRSRNVPVPHPLPSLVGLLESTKRVKRRPSSDYSSLNSRHSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X724</accession>
    <name>A0A8I6X724_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X724"/>
    <dbReference type="SMR" id="A0A8I6X724"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164330"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0373830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0373830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0373830"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0164330.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0164330.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0164330"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0373830.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0373830.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0373830"/>
    </dbReference>
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        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="184"/>
            <end position="222"/>
        </location>
    </feature>
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        <location>
            <begin position="120"/>
            <end position="161"/>
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        <location>
            <begin position="187"/>
            <end position="203"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0164330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="222" mass="24444" checksum="19F1CADFE8E5B40F" modified="2022-05-25" version="1">MYDLYAMAHTASYKKVKAFSESDLEHPENFTNISSHDKLVKYRDHGKARKGEDFNPSEGPIDPELVMIAGNGRPHGSIAIGDGLIRCPSTLRQIKARQTSSCPEITRRPRPVELAIEAAIQKEREAMQAAIAEKDKEIRELEERTTALMEAERARNDASNRAIYELFVSMCEKSGQVPPPMPVINVAGTNISRNASHDPSTVEPSPGQPSPGETSQSHRASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X7F6</accession>
    <name>A0A8I6X7F6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123427920</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044967992.1">
        <property type="nucleotide sequence ID" value="XM_045112057.1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0198130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0198130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0198130"/>
    </dbReference>
    <dbReference type="GeneID" id="123427920"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0164610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0164610.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0164610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0198130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0198130.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0198130"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427920"/>
    <dbReference type="OrthoDB" id="671551at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="285"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="541"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="812"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0198130.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1483" mass="167967" checksum="71C16E164CFC46C4" modified="2022-05-25" version="1">MDLALNAAQWVVGKALAPVADGLLQSWAGSNQLGPNMEALRMELLLVKATLENAGDKQLDGRQALEELLQKMQDLAHNAEDVLDELDYFRIHDELYGTCDAANEDTRGCGHNVLLNARHTAKAVGKLLSCTSASTSGDHVEEDATHRVLCCAPPRARERARGSSSSAAGEEVSSRMSKFGKFFPCSSIPKVHDDDDSGNSILSGVHARKVPQNNPANETTILRFHRVDVSNRMKQIVEQLRPMRQEVTTILRDCRLRIAAGPAHNRPTTTSESLEPKLHGRDHMLNSIIHDITKGKYCGKDLTVLPLVGPGGIGKTTLTQYIHNAQEVQNHFEVVVWTCVSLDFNPNKLLEEIKGKIPNVEGEKEGEVGKVIEQRLKSKRLLLILDDMWKCGDQEDWERWLLPFKKSQTKGSMILVTTRFQALAETVKTTGRYIKLGGLEPQEFRKLFLAYIFGDEDSTEDHTYLLETGDKIIEKLKGSPLAAKTVGRLLRDHLDLHHWKMVLESKEWETQTGNHDIMPALKLSYDYLPFDIKQCFSYSALFPEDYKFHSKQLIHFWIGLDILHSSGETRTKEDIGLRNLNYLVTHGFFRKEETSGEPYYIIHDLLHNLALKIASHECLSLNFPNVRSIGIHPCTRHLSVIIDIVDCDKAMASENIETELRKLKTRFSVEKLQTLMVFGEMDESIASIFGDFLREANALRVLFFSKMCIPVESMLHNFSTLVHLRYLSLGTMLKMYLPDTISRFYHLKILDLEEWCGYHDVPRDMSNLAKLCHIPTQRDELHSCIYNVGKLKLLQELKAFKVNKENEGFEQKQLEDLVELRELGIYNLEKIHTQEEAAKAKLINKNYLRVLTLSWDSEQTNAKPEDEGLVLESLQPHRNLQELYIRGNKGPSCPTWLGDKLDVEALKSLHLFRVSWDVFPSFGKMWDLRELTLNHISTIKEFGLEESLCKLLKKITLTGLENFEKWVPQPTHFFPHLQVLIIIDCPKLSELPFSCHIVYPLKQDWNTDWFPKLQELKIENCPEVLLLPPIPWTQSLCFVKINDVGSKLLHDLVYSKSSSGVALNIHGKDGLLSFDQLLLFSQLTELQELNIYNSPPLELKYFLMLTSLKTLLAWSSNLGVVPSEVQSGVEWQHPVDEIKIWSSDSSGKELSQFLSHLPKLSKLEVNSCKNITQLAVGVDLQQTTVPVVSSSISLDVITMDDTQTQVKVEQQEIAEVEKEEANTVDDDGLLLLPAHLSNSLQELAIDSGNLVVHSLNALKALTRLELKDCSFRHPFPSCLLDLELSSVEGVLTLSNLTSLTRLEIYRCGEDFRCEGLLPLLTRGHLSSLEVIGSPNFFGGWEWDPNAMWGRSSKLQKVETDDIQGFLGAPALCSLLSSSLSDLHFSWNDETAHFTNEQEEAFQCLTSLQVLYFWFCHKLEHLPAALNKLTNLKRLVICGCPALRSLPKDGLPSSLRELDVEDCVNKELTEHCRRLMGTIPKIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X8F5</accession>
    <name>A0A8I6X8F5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">cinnamoyl-CoA reductase</fullName>
            <ecNumber evidence="6">1.2.1.44</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Aromatic compound metabolism; phenylpropanoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.44" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044948449.1">
        <property type="nucleotide sequence ID" value="XM_045092514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6X8F5"/>
    <dbReference type="SMR" id="A0A8I6X8F5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0436890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0436890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0436890"/>
    </dbReference>
    <dbReference type="GeneID" id="123398004"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0436890.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0436890.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0436890"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398004"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016621">
        <property type="term" value="F:cinnamoyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000199">
        <property type="entry name" value="Cinnamoyl-CoA reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF653">
        <property type="entry name" value="OS08G0277200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0436890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="37071" checksum="21C517A7880C5480" modified="2022-05-25" version="1">MGIDCANTANCVATGRGRTVCVTGAGGFIASWLVKLLLEKGYAVHGTVRNPDDVARNAHLRGLEGAAERLTLFRVDLLDKESLVAAFRGCQGVFHTACPVTDDPEKMIEPAVSGTRNVINAAAEVGGIRRVVMTSSIGAVYMDPRRSPDEEADETCWSDLEFCKNTKNWYCYAKTVAEQAAWELAKERKLDLVVINPSLVLGPLLQTAVNASTWHIAKYLDGSVQTYANAAQAYVHVRDVADAHARAYETPEAHGRYLCAGRTLHRAEVCRTLAKFFPEYPVPMRCKEGTGEMKKGCRFSSRRIMELGVGITPASQCLYDIVISLQDKGILPRRGADMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6X9N0</accession>
    <name>A0A8I6X9N0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NBS-LRR disease resistance protein homologue</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="SMR" id="A0A8I6X9N0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0086450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0086450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0086450"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.1HG0071130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.1HG0071130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.1HG0071130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0086450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0086450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0086450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="9">
        <location>
            <begin position="176"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="11">
        <location>
            <begin position="443"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="12">
        <location>
            <begin position="562"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="12">
        <location>
            <begin position="786"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="13">
        <location>
            <begin position="993"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1439"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1448"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1475"/>
            <end position="1486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0086450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1486" mass="169183" checksum="89321E8F1F313A5D" modified="2022-05-25" version="1">MTGFGEIIASAVGKQVAGKLGELATEEATLQWKFKDDVDVMADKMQDLEAVLHDADDRLRRRGRDGEAVGRWLSKFKAVAYDVEDVLDDLDATELLKKSQPKLKLFFSWNNQLLQRVTMPHKLKSVRGKIEKIRKEGQAINLVRHQERAEGSRKNETFAGICDEGLKSGVVGRDIEKDKIISLLLKKGVQEDISIVPIVGLGGLGKSTLAESVFVDKMVNDFEVRAWVHVSKKFDLRKIGSAILKSINSSINLDNCSLQFLQENLRNELADRRYLIVLDDLWEEDADKLERLKQMLQHGYRGSRVIVTTRNQSVVNKLRTGALSHKRKIRLVPKSDQIKLHGLSNDDCWEVMKQTAFGPDDDKSDLEEIGRKIAEKCGGVPLVAIALGQVMSELRTVEAWQTLRDTNIDLGHRDHEDTLERLMLSYYYMKLDFKMCFTYLAAFPKGFIIESDRLIQQWKALGYIHRGDDGKRCINYLMGMSFLQISRSSAIRSNPVHANAPRELTMHDLVHDLATIISGHESLVLNATEPRSRKKAKRRYCRHAQLINYQNQYKVFNDLPSKIRSLHVRHSEKQQLPQKAFSRTKYIRVLDLSGCLVVGQSTPSNLFLPSSTFQLKLLRYLDASSLHITSFPKSFHTLQNMETIILSNCSLKTLPDSICSLNKLLYLDLAGNARIIKLPENFHLLNKLRFLNMSSCSKLTELPDNFSLECLEHLNLSGCHELKYLPQDFGNLQHLRFLNLSNCYNISILPESCCQLEHLKDLNLSDCRALVVLPEGFGNLSGLESLNLSGCYKISVLPESFCQLTHLKELDLSDCRALIVLPEGFGDLLELQSLNLSDCYEVSVLPESFCQLKHLKDLNLSDCRALILLPECIGDLSELESLNLTSCPRLARLPESVCKMTNLKCLNLSYCLGILELPSSVGDLNLQILDISAGALRHLPDSISEMASLTQFVVTSGHPRVFGEAQEIKKRLKLPGRTVHRAYQRPSGRNSIVELARVNCRELLIGSLQCVEKLKDLERVELRHKSFVRQLALHWNQQDMNASRKDMNQQDINHLRNRKMNAIECSAQSILERLIPARNLEQLMIQGYSSKGFPNWMSHISSHLPCVTYLSLSDLGACDDLPPFGQLPNLRSLYLKKIPKVTKIGKEFYGEGGTCTKLRLLQLKSMDNLAVWYTTRSGEEDGEFLIPNLHNLELKDCPLLEFFPYPPRSMFWCLDNSNEVLAGQGFGKLSSSTHPCRMGIKNCSFRPDKWRRLEQLPTLEEFSLTSCRSLRALPEVICCFTSLKKLSLMSLTDLETMPEWLGHLTSLQVFALKDCCNLTFLPGSMKNLTALRVLMLLECKGLYILPKWIGELSSLRELHIRDCPNITSLPESIRNLTTLEEMYISGCSSLVHRSQREDAYKVSHIPKVILEPEEPNEEQREEDSQEAYTDRLLKNYRNNYWNYGENPSRQREEREELESRMSDSDSEHLSRYGEEDSENEDDEQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XHZ0</accession>
    <name>A0A8I6XHZ0_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123448573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044981457.1">
        <property type="nucleotide sequence ID" value="XM_045125522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XHZ0"/>
    <dbReference type="SMR" id="A0A8I6XHZ0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0372590.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0372590.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0372590"/>
    </dbReference>
    <dbReference type="GeneID" id="123448573"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0310150.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0310150.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0310150"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0372590.2">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0372590.2"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0372590"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448573"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="67"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0372590.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="583" mass="63667" checksum="5801CEB9B543E747" modified="2022-05-25" version="1">MDQLVNFIIRPPRADYSPNDDLLEQEFMLKGRWFQRKDLEVVNSQGKKLQCSHYIPAVIPEGTALPCVIYCHGNSGCRADASEAAIILLPSNITVFTLDFSGSGLSEGEHVTLGWNEKEDLKAVVNYLRTDGNISCIGLWGRSMGAVTSLMYGAEDPSIAGMVLDSPFSNLVDLMMELVDTYKYPLPKFTVKLAIQHMRKIVKKKASFDIMDLDTIQVAKRCFVPALFGHATEDDFILPHHSDKICEAYVGDKNIIKFDGDHNSPRPQFYFDSITIFFHNVLNPPDVPDDHYFLTPHGSLGQGHWDTEHDIEYRIAQSPTAPSTTTEDAIAQLRSRRLMSRMEVPSGTTPEGRADRTEVLDGDVGPSSSSASTVTPPNGHNGRMLTPTSDDGEYVEYSFDSVSDMPYTEEDEDRMLMQAILESLRDLDKSNTKKTQSPVSDAASKENSVAQDGKPTPDVVALETDASSISVGGADAPGKKDVAACNSVAKTGDVQSADDCTVSDAVCADVSGTSESNDSTQVMNGESQKITQDMNGKFGSQKPTQNPNGEDGTRATLVVQKSRTGSLIDGLAQKWGSFFKNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XI50</accession>
    <name>A0A8I6XI50_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XI50"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0432100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0432100.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0432100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF133">
        <property type="entry name" value="OS07G0196600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0432100.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="422" mass="47478" checksum="3A33E13440A60FDF" modified="2022-05-25" version="1">MMEGKRPTHHGGYEMSEDLLMEIFSRLPAHSAARCAALSRRWRKIVDGPDFWIRHHRPPSPNFNMAYIHEYTGEGDHRPLQHAFHLPGLLAQGLLSNDRHGLAYRYVGTCNGLVALQVEGLYHHSFFACVVLNPATTEEATLTYLGLCEDHYLCGFGYGPASKTYKLLVVRRPYGCRYGGPLEVLSLGDARGEARAVFPNVWCNDTFSLCIGGVLYVLVDKHLSVLALDVDSEAISAVELPVGRVVCSEPMEVRGRLCIAMHTDETHEFVLWVLTASLDWERRCVLRYMVPTTLRSHWLVGAWDSGENRLLVWYRGAGLRLYDLREMDSGGAHEPAGSWTLEDQKDLRDYMRWGYGQYRLCWGYRPTTISPRVTIANCAGVPPVLCSFRSGRQTPQRPVAPFSNVLLDLVKTILVGQEVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XIW3</accession>
    <name>A0A8I6XIW3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123447512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        </location>
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            <begin position="477"/>
            <end position="493"/>
        </location>
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            <end position="510"/>
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            <begin position="735"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="primary">LOC123439947</name>
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    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0270320.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="3">
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    </dbReference>
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        <location>
            <begin position="26"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0402470.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="189" mass="21020" checksum="B8B962C000B4895F" modified="2022-05-25" version="1">MNSADTKARFEAYNRLQAAAMAFGEKLPIPEIVAIGGQSNGKSSLLEALLGFRFNVQEVEMGTRRPLVLQMVHDPTALDPRSRFQEEDSKEYGHPMVQVAAIADLIKQRTESHLRTIKAAASPKPIVMRAEYAHCPNLTIIDTPGFVLKAKKAEPKRTPEEILSMVKTLASPPHRLILFLQQSSVEWCS</sequence>
</entry>
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    <accession>A0A8I6XQM0</accession>
    <name>A0A8I6XQM0_HORVV</name>
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        <recommendedName>
            <fullName evidence="2">TOD1/MUCI70 glycosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044977038.1">
        <property type="nucleotide sequence ID" value="XM_045121103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XQM0"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0288840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0288840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0288840"/>
    </dbReference>
    <dbReference type="GeneID" id="123444405"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0239790.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0239790.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0239790"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0288840.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0288840.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0288840"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123444405"/>
    <dbReference type="OrthoDB" id="1905162at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006852">
        <property type="entry name" value="TOD1_MUCI70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048354">
        <property type="entry name" value="TOD1_MUCI70_glycTrfase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956">
        <property type="entry name" value="ALKALINE CERAMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956:SF17">
        <property type="entry name" value="OS01G0749100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04765">
        <property type="entry name" value="TOD1_MUCI70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="TOD1/MUCI70 glycosyltransferase-like" evidence="2">
        <location>
            <begin position="150"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0288840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="459" mass="51812" checksum="5F5D4BAECED1B650" modified="2022-05-25" version="1">MPSSTIRSISITVSDDDGAAAPRRARAGRRKVVRSLGQRAVRLVARWWPILLLLPAVALLLFEASRLRASPSPAPPVSSSLGRLDPTTRLVHGVREPCLKFLNPKSIENLAFHEGSGLDAVVKRIIYKTDDDDYDSHHSEANSTYLLQHAEATRFNLFTGFQTVSEREESFKVNETVNVHCGFYSDNGGFKISDDDVRYMRTCKVVVSTCAFGGGDDLYQPIGMVNSSIGKVCYVAFWDEVTLSTQEAEGKLTGDNEKIGRWRIIVVKNLPFVDQRLNGKIPKMLTHRLFPEASYSIWVDSKYQFRRDPIGVLEALLWRRNSTFAISEHGARTNIYDEGKAIVQKNKATPEEVKVQLTQYRQDRMPDGKRLHGLKALAEASIIVRELTPLTNHFMCAWFNEVVRFTSRDQLSFPYVLWRLNMPGIYMFPVCTRRDLVNSLGHTRKVKPLTQTNSDSSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XT11</accession>
    <name>A0A8I6XT11_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XT11"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218960"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0182290.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0182290.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0182290"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0218960.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0218960.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0218960"/>
    </dbReference>
    <dbReference type="OMA" id="CERQILD"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035286585" evidence="1">
        <location>
            <begin position="24"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0218960.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="134" mass="14499" checksum="3311AC85997DBFE9" modified="2022-05-25" version="1">MAAGGAFLLVFAVFSMLVMSSLGNPRPLCSDCETLCRTNCTAEVETSCSSTCNDNPLEGCRRQVFERCTTDGTCCSSNGTCTCDCNTVSQDRCRGITDNYTDCQACERGQFDYCYSTCKSGCNNNCKKKKGCHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6XTK9</accession>
    <name>A0A8I6XTK9_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6XTK9"/>
    <dbReference type="SMR" id="A0A8I6XTK9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0577610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0577610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0577610"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0577610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0577610.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0577610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0577610.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="124" mass="14001" checksum="B4F32BACF83E954D" modified="2022-05-25" version="1">MEKRKDAPVPEGEEPKYDAKIVEEVLMTEVKESTFLRNVGLKSSSYNSGKGTAAVAAHVRDLEQKLERSELQDEVMQEEMAAIKMKAEEYEAARDKELELMCKKSQEHEQKLAHLMALFGAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0B6</accession>
    <name>A0A8I6Y0B6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC123398563</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044948957.1">
        <property type="nucleotide sequence ID" value="XM_045093022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0B6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0470480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0470480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0470480"/>
    </dbReference>
    <dbReference type="GeneID" id="123398563"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0389580.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0389580.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0389580"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0470480.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0470480.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0470480"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="200"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="556"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0470480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="63330" checksum="D2C03AC6B8127CC7" modified="2022-05-25" version="1">MKYMKLGSKPDTFYTEQAVRSVVSDIPADLIIHVNNTKYQLHKFPLLLKCGHLQSLCSDTEADEQLPVPVALHDIPGGEEAFEICAKFCYGIAISISASNFVPAMLAARFLRMTEHVAKGNLVSKLDTFFESCVLHGWRDSIAALQAAWRISGWSESRIVQPCVDSIVEKILLPPSKVAWSYTYTRPGYAKRAHQSVPKDWWTEDISELDIEVFRSVVSTVRATRMLPSPLIGEALHVYACKHLPDPLYTGGSANGHASQSQSSSSTAAAAEEALAKQRRVLETVVTMIPSDVGSVTGRFLLRLLRVANYVGASSSTRAQLIRQAGSQLDEAKAVDLLIPLPSDPQAYDVGAAEAVLEHFLTQFQRPAAPEERRRMNVAMEKVVRIFDEYLQTIALDSEFPIGRFIDLAECLPGIARNNHDGLYHAVDTYLKEHPDLSKADKKRLCRLIDCRKLSTDVRAQAVSNDRMPLRTIVQLLFVEQERTTGAGGSHSVVPPDRASVDTVSRLTATGREDEAADMDHKSDVHRPRRGGREERAQSEAAAMTRSLSASTKTAARKDRTVEERGSRLRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y0W6</accession>
    <name>A0A8I6Y0W6_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Flavonoid 3'-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123403301</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044953187.1">
        <property type="nucleotide sequence ID" value="XM_045097252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y0W6"/>
    <dbReference type="SMR" id="A0A8I6Y0W6"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540900"/>
    </dbReference>
    <dbReference type="GeneID" id="123403301"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0449850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0449850.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0449850"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0540900.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0540900.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0540900"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123403301"/>
    <dbReference type="OMA" id="HKTLHEM"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000207">
        <property type="entry name" value="Putative cytochrome P450 superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF18">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0540900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="56685" checksum="D8789851EE63B12C" modified="2022-05-25" version="1">MEIPLPLLLSTLAISVTICYVIFFFFRSDKGCAPLPPGPRGWPVLGNLPQLGGKTHQTLHEMTRLYGPMFRLWFGSSLVVVAGSADMAKLFLRTHDAKFSSRPPNSGGEHMAYNYQDVVFAPYGPRWRAMRKVCAVNLFSARALDDLHSFREREAALMVRCLADSAAVGRVVALAKAANVCTTNALSRATVGLRVFATAGSELGAEDFNEIVLKLIEVGGILNVGDFVPALRWLDPQGVVAKMKKLHRRFDDMMNRIIAQRRAVSTTAGKDLLALLLAMVQEDKSLTGVEEDKIRDTDVKALILNLFVAGTDTTSITVEWAMAELIRHPHILKQAQEELDAVVGRDRLVLESDLPHLTFLNAVIKETFRLHPSTPLSLPRMAIEECEVAGHRIPKGTQLLVNVWGIARDPTLWPDPLEFRPARFLPGGSHAGVDVKGGDFGLIPFGAGRRICAGLSWGIRMVTVTTATLVHSFDWEMSAGQMPDMEETFSLLLQLAVPLMVHPVPRLLPSAYEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y106</accession>
    <name>A0A8I6Y106_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cas1p 10 TM acyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123448798</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. CASD1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044981750.1">
        <property type="nucleotide sequence ID" value="XM_045125815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y106"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0381260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0381260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0381260"/>
    </dbReference>
    <dbReference type="GeneID" id="123448798"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0381260.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0381260.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0381260"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123448798"/>
    <dbReference type="OrthoDB" id="1932925at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012419">
        <property type="entry name" value="Cas1_AcylTrans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533">
        <property type="entry name" value="N-ACETYLNEURAMINATE 9-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533:SF48">
        <property type="entry name" value="PROTEIN REDUCED WALL ACETYLATION 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07779">
        <property type="entry name" value="Cas1_AcylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Cas1p 10 TM acyl transferase" evidence="9">
        <location>
            <begin position="112"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0381260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="552" mass="64016" checksum="CCB58879B7CD6038" modified="2022-05-25" version="1">MAEALASAGGLAMAASTSFTPGQLSAFLGFLWVLAAWVYAEVLFHRKNAASLKTRHSDINLSDMDSSSNKGEDQTMLLEEGGQVAGAKPVYASFTSQMLRLFCMDQTLLLEHRLTLRAISELGGHLLYFYICDRTNIFGESGKNYSRDLFLFLYFLLIIVAAITSFKIHQDKSTFTGKSILYLNRHQTEEWKGWMQVLFLMYHYFNAKEIYNAIRVFIAAYVWMTGFGNFSYYYVRKDFSLGRFAQMMWRLNFFVAFCCIVLNNDYTLYYICPMHTLFTLMVYGALGILNKYNEIRSVIAIKFVGCFLVVILVWEIPGVFEIVWSPFTFLLGYDDPSKPDLPRLHEWHFRSGLDRYIWIVGMIYAYYHPTVEKWMEKLEEAETRTKLYIKASIVTVSITAGYLWYEYIYKMDKITYNKYHPYTSWIPITVYICLRNFTQEFRACSLTLFAWLGKITLETYISQFHIWLRSKVPNGQPKWLLTIIPDYPLLNFMLTTALYVAVSHRLFELTNTLKIAFVPRDNKHLSYNFVAGIAISVALYSVSFALVGIAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1F1</accession>
    <name>A0A8I6Y1F1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin receptor RAD23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA repair protein RAD23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123396360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RAD23 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044947248.1">
        <property type="nucleotide sequence ID" value="XM_045091313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1F1"/>
    <dbReference type="SMR" id="A0A8I6Y1F1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0479410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0479410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0479410"/>
    </dbReference>
    <dbReference type="GeneID" id="123396360"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0397220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0397220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0397220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0479410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0479410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0479410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000069">
        <property type="entry name" value="UV excision repair protein RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.540:FF:000001">
        <property type="entry name" value="UV excision repair protein RAD23 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF59">
        <property type="entry name" value="UBIQUITIN RECEPTOR RAD23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="8">
        <location>
            <begin position="162"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="8">
        <location>
            <begin position="345"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0479410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="395" mass="41833" checksum="910154E59B12A284" modified="2022-05-25" version="1">MKVSVKTLKGSKFEIEVNPADKVSDVKKLIESSQGQNVYPADQQMLIYQGSVLKDETTLEENKVVENNFLVIMLRQNKGSSSAAPAKSKEPSNQAPPTQTVPATPASQAPATPAPQAVSAPAPIVPVSAPAPAATASPAPAVAVSTEAETYGQAASNLVAGGTLEATIQSILEMGGGTWDRDTVLRALRAAFNNPERAVEYLYSGIPEPMEIPAPAPSAQPADPAQASLATQPAVASSGPNASPLDLFPQALPNASTNAAGEGNLDVLRNNAQFRSLLSLVQANPQILQPLLQELGKQNPQILQLIQDNQAEFLRLINEPAEGDEDENLLEQFAEGVPQTIAVTPEENEAILRLEGMGFDRALVLEVYFACNKDETLAANYLLDHMNEFDDGAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y1L1</accession>
    <name>A0A8I6Y1L1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC123397437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044947905.1">
        <property type="nucleotide sequence ID" value="XM_045091970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y1L1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480450"/>
    </dbReference>
    <dbReference type="GeneID" id="123397437"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0480450.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0480450.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0480450"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123397437"/>
    <dbReference type="OrthoDB" id="251770at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="CDD" id="cd17731">
        <property type="entry name" value="BRCT_TopBP1_rpt2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059215">
        <property type="entry name" value="BRCT2_TopBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47576">
        <property type="entry name" value="BRCT DOMAIN DNA REPAIR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47576:SF2">
        <property type="entry name" value="BRCT DOMAIN DNA REPAIR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="51"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0480450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="549" mass="61247" checksum="945728D4C530D76D" modified="2022-05-25" version="1">MARCGVEVIASRGCSRLLLPGMQPSSASAASSSSVSVTRSAAATDAAARRPPDGPFAGLVVCVTGLSKEARSQVKEAAERLGGDYSGSLHPKCTHLVVQSFAGRKFEHALKHGPRNGLFVVTLGWFVDCVRRNMRLDESLYSIKNIGENGLPLGEYNRLVGLPVNEKSCLPPMIFQDKACSGTTHKHQLQPHREEPEHDVFVFTNDTIYIDPGISGDMKKKISDAATSEDAKLLDHWFIGCHAKYVVCEDASVKRYVGHSDNIVTPFWILKTVKEKNLQRLVHLSSDLARHVAVVLENFHTSQENRKLGSAPSLNSSELPTQGEIDEARQERQKFVEVAKKDIRDRRVRRMQSCEVPIHPLTPVTLLDSICWTISEPVSSASIYMDSSWSEDADEQQGTTYFDADGDGKDRDQSADNLSRPLRESERSEVIFKNHFFTILFPLDRFGELGPSSRTFFSNGGFTREQVLDHIYNFYQDNMSTDEINVALHTDSRHADRLRSLYGSAECAGSGFVIAFKRVDFLGSRRSFEALKRLSRENNSNVYELVIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y2I3</accession>
    <name>A0A8I6Y2I3_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Patatin</fullName>
            <ecNumber evidence="8">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123398678</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="function">
        <text evidence="6">Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Hydrolyzes phospholipids as well as galactolipids. May play a role in disease resistance.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044949061.1">
        <property type="nucleotide sequence ID" value="XM_045093126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y2I3"/>
    <dbReference type="SMR" id="A0A8I6Y2I3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0516310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0516310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0516310"/>
    </dbReference>
    <dbReference type="GeneID" id="123398678"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0428970.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0428970.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0428970"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0516310.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0516310.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0516310"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123398678"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07199">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241:SF12">
        <property type="entry name" value="OS03G0784100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="67"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0516310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="445" mass="45990" checksum="07E6E56CA65083E1" modified="2022-05-25" version="1">MEQAPAGPAAAMDVDKLTYEIFSILESKFLFGYDDPKLLFAGGSPHTPPAAAKDTPPRVQAGKVCILSIDGGGRAADGLLAGAALVRLEASLRRRTGDPKARLADFFDVAAGSGAGGVLAAMLVARGKDDARPLYSAEDALAFLVRSLRRGWSSSSGSLRALLRRPAGGAAFRKVFGDLTLRDTARPVLVPCYDLATGGPFLFSRADAVENPAYDFRLRDVCAATCAGSDRSSSAVEVRSCDGSTRIVAVGGGVALGNPTAAAITHVLNNKREFPLASGVEDLLVISIGSGEGEQRPAAGTGGASTSEIVRIAAEGVSDMVDQAVAMAFGHNRTSNYTRIQAMGSPRGGRCAAAAAKGCVAAEEMLSQKNVESVLFCGKKLAEQTNAEKLERFAHELVKERDRRGSSALGAAAPAVVKQQAAAAAGDAAPASYSNLVSQMFTSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y373</accession>
    <name>A0A8I6Y373_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123449900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044983199.1">
        <property type="nucleotide sequence ID" value="XM_045127264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y373"/>
    <dbReference type="SMR" id="A0A8I6Y373"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413180"/>
    </dbReference>
    <dbReference type="GeneID" id="123449900"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0344280.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0344280.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0344280"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0413180.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0413180.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0413180"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123449900"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0413180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="168" mass="18359" checksum="83F9CD4B67BF35AE" modified="2022-05-25" version="1">MAPRGAVELRRIEDRTSRQVRFCKRRSCLFKKAYELGLLCDAEVALLVFSPAGRLYEYASSSIGGTYDRYQAFGGDEKDHLNEGGASTNNDGDPSNIQSRLKEIASWSLQNNADEADANELEKLEKLLTDALENTKYKKMLAQRNGGASTGSGGGNSISPMGQKEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y527</accession>
    <name>A0A8I6Y527_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y527"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499720"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0499720.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0499720.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0499720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074:SF42">
        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1618" evidence="2">
        <location>
            <begin position="243"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0499720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="316" mass="35163" checksum="0B273015AC05AEEB" modified="2022-05-25" version="1">MALIFNGDGTYSMVYPEGTSPMIWLRPADRPKTDPTLSPPKSVLLDTLGYVDGECNDTTAAYVAADGRRVQVTFWMHHPPLVSCFTVHSPGVKPDAFPEMAKLLATDGDLALLRVPHLPLNPFSDQPDSRFFVYRIGGGAAGEKPPWLRQLPNPDKLMFTDHTVGFLNYGTHGQADDRFFAAAFYQEHPLRPGGSVKFYISLFDSETWGWTRKEVFVDSPEAYRYRFTNKAITIGGDRGSIAWVDLSNGILVHDLLLRNDILRYIPLPPSELPDLRTNSVRDMAVVNGYLKYVSMSIAGLVVVSVEYCGLVVAVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y6A7</accession>
    <name>A0A8I6Y6A7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123452559</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044985160.1">
        <property type="nucleotide sequence ID" value="XM_045129225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y6A7"/>
    <dbReference type="SMR" id="A0A8I6Y6A7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0427780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0427780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0427780"/>
    </dbReference>
    <dbReference type="GeneID" id="123452559"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0355340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0355340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0355340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0427780.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0427780.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0427780"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123452559"/>
    <dbReference type="OrthoDB" id="1907171at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009524">
        <property type="term" value="C:phragmoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000052">
        <property type="entry name" value="Kinesin-like protein KIN-12F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF16">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="85"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="516"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="562"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="614"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1085"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="986"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="644"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1087"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1105"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="162"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0427780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="1121" mass="124280" checksum="FAD6B21D84E21A3A" modified="2022-05-25" version="1">MEMLRRNLKRQTSRSRSLSPLSVASPAHDQENLHPNLAAAPAPPSPSTKPSTPGLSKPVPTAAAVPAPHPPAAPAQQKPADDEPAVKVVVRVRPAVSLPVDGKDLFFVRRTSPDSIAVGDRAFAVDGFLDDRASQEDVFQQVGVPMIHSALAGFNTSLVCYGQSGTGKTYTMWGPLAAMFDNRSDRADRGVVPRFFRSLFSQIQGNQESSPEKHTSYQCRCSFLEVFNEQINDLLDPSQRNLQIRETTGNGIHVENLTEEYVSTVEDVNQILMKGLSKRKIGADSMNLKNSRSHVIFTCVIEAWSKDLSSNGFSSSKTSKITFVDLAGVDIDESDGAGKNITREERHVKKSLSSLGKLVNILSEEPKPQEDELSYSQSRLTHVLKDTLGGNSRATFLCSISSEHRYRSETLSTLRFGERAKLMPNKAVVNEISEDDVNGLSDQIRQLKDELVRTKSGEHATSETGYFNAQIARASLHTLRVSLNRSIILPPIEVEAEDEMDVDEDDVHELRDQISKIHSSSEDTLDDFMDAESGEDSPRLEENPRPWEHDDQVVIDDSEGPLQEEPQKMHSNTSADHDQVSDRKSVLSINASEQLSPIQDAMFCSSPKIHKARKSIASPGFSPSKLSESSPGEADLETYRKSAVRSSLQSSKLSPTDSLAASLQRGLHIIEYHQQNPPAPRKSFVGLSFDHFALNPRQSAKASSALQALPEGDASSVSTICSSCKKAMDTNDDLSEDINSEKQIVTATAVTSNDLANASLKDGNISSTTDSKRVAELEAVCEEQAAKIKELSNLIEQQKKGSEDGEQTAEDKIAEQCEDSRTPLDVYEREALEGEIQKLKDQVQLLTDGSTASDSLLDQIRNGSTDQEYELEKERQKWMESESKWISLTEELRMDLESSRKHAEKTEAELHEEKKCTEELDDALQRAIYGHARIIEHYVELQELYDDLLERHRGVMGGIAEVKRAAARAGKKGCGTAFAAALAAELSTVRIDREKERAQLKEQNRRLRVQLRDTAEAVHAAGELLVRLREAEEASTQEKERSAAMLQENQKLKKQLEKTRKKHEVEIETMKHYLAESRLPESALEGFYRHESGEDAHAHAPSTAGHDDDQSWRAAFKSEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Y7Z8</accession>
    <name>A0A8I6Y7Z8_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A8I6Y7Z8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676130"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0561050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0561050.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0561050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0676130.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0676130.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0676130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF75">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0676130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="424" mass="47218" checksum="9A43800F7EE72B7A" modified="2022-05-25" version="1">MRRAHDTRAVLSQPARNRLYNAPLHPRFRGTAQHIRRPRRHRTKRIGGGRQHLCRTDPPASEQGTPAGSGGDQPVGNLLSSSSLPPPRLRDMGANGALEEERPRADGGDEEGGATVFRGTNYSLPRTIAALALWLGGIHFNVLLILASLFLFPLRLAALVVALQLMFMFIPLNDEDKLGRKIGRFICKYAMGYFPISLHVEDYEAFDSSRAYVFGYEPHSVLPIGVAALANHVGFMPLPKLKVLASRAVFHTPFLRQIWTWIGLIAATRKNFYSYLAAGYSCVVVPGGIQEILHMDHDSEVAFLKSRKGFVKIAMQSGCPLVPVFCFGQSKAYKWWRPGGKLFVNIARALKFTPIIFWGRYGTPIAFSSPMHVVVGRPIELKKNPLPTIDEINEVHGQFIGALQELFEKYKTKAGYPGLHLRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YAL7</accession>
    <name>A0A8I6YAL7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044979821.1">
        <property type="nucleotide sequence ID" value="XM_045123886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YAL7"/>
    <dbReference type="SMR" id="A0A8I6YAL7"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409560"/>
    </dbReference>
    <dbReference type="GeneID" id="123447297"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.4HG0341100.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.4HG0341100.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.4HG0341100"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.4HG0409560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0409560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0409560"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123447297"/>
    <dbReference type="OrthoDB" id="406156at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 4H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000041">
        <property type="entry name" value="Hyoscyamine 6-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="184"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0409560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="339" mass="37827" checksum="2D53BACFB50FDD59" modified="2022-05-25" version="1">MENILHATPAHVSLPESFVFASDKVPPATKAVVSLPIIDLSCGRDEVRRSILEAGKELGFFQVVNHGVSKQVMRDMEGMCEQFFHLPAAVKASLYSEERHKPNRLFSGATYDTGGERYWRDCLRLACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIRADYFEGDLSGGNVILNINHYPSCPNPDKALGQPPHCDRNVITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNFGQQLEVVTNGLLRSIEHRAMTNSVLARTSVATFIMPTQECIIGPAKEFLSEENPPCYRTIMFRDFMRIYNVVKLGSSLNLTTNLKNVQKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YBV3</accession>
    <name>A0A8I6YBV3_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YBV3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.4HG0341230.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.4HG0341230.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.4HG0341230"/>
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            <begin position="56"/>
            <end position="68"/>
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            <begin position="77"/>
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        </location>
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        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="190" mass="20333" checksum="B9DF6129699F0565" modified="2022-05-25" version="1">MWHYAGPSDSTRSHPEDVSGETMSAWVRGITGACDNPVGARRVKPFRAENPPPNEEWTNWHSAVSNGNPAEEEEGSQEGSADSAEYVSDSGETEEETEEEEEEDGEQSSPPSPPEHRTKRRHDPAAPPAPPAAPSAPPAPPVAPSARSVKRTRDATAEPVNQPSKMAKPSGPKPRKALPRMRIAVPVAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hordeum</taxon>
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    <reference evidence="2" key="1">
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            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YCC8"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0574080.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="220" mass="25757" checksum="A97BBBE10B7407FC" modified="2022-05-25" version="1">MELLEMKKLSIPDIKVTKRKYGFWGVRTKVFGRKDDPKTEDINYGTLTHDCDMGMNIAVHDAITRLSYRRHVELGTRYFGRLGWQKPEGSHIILTNEQKVKFSPALVYNQELEHYIKNLQMDLLSELVENDALRESLKTEKLKVVNVKKHDPEIQKPHQDLKNSEAMADAGDVEIQKLKRQLLGLQGRHRKLRVNREYLAEELDNIKHDQKKPIDDEDMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
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    </protein>
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    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
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        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Schulman A."/>
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                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044950703.1">
        <property type="nucleotide sequence ID" value="XM_045094768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YEY2"/>
    <dbReference type="SMR" id="A0A8I6YEY2"/>
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        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0610780.1"/>
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    </dbReference>
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    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0506510.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0506510.1"/>
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0610780.1">
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    </dbReference>
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    <dbReference type="OrthoDB" id="40134at2759"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:amino acid transmembrane transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
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            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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    </feature>
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            <end position="343"/>
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    </feature>
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    </feature>
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            <end position="464"/>
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    </feature>
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            <end position="462"/>
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    </feature>
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            <begin position="1"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="Pfam" id="PF01490"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YL76</accession>
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            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="SMR" id="A0A8I6YL76"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0606560.1">
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    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.6HG0502870.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.6HG0502870.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.6HG0502870"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.6HG0606560.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.6HG0606560.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.6HG0606560"/>
    </dbReference>
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        <property type="component" value="Chromosome 6H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF37">
        <property type="entry name" value="NO APICAL MERISTEM-ASSOCIATED C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.6HG0606560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32528" checksum="FE2FAAA2015D0DA8" modified="2022-05-25" version="1">MEASAPSAAASMANYCRIKPKNSGFISRSYRSPQGRWDLMKPACARWSAAMDQVRDAPPSGTVESDYETIAGMRYKEMAASKGKPFPFKHAWAILQTFDKWKFRDQETAPKKSAMLRMDDSEDEEKERNLGKPEGTKKGKQRAKMEGEASSLREKMEQMMKAREELTRKTLETKILITEKKKEVKLAQVKAKREEAKRKADLEERMIKVKEANVWKELMVEEHMMMSKKDMDQEQLQWWKDYKEDIAERKRIFRDASSTLRGDTSMSGGGDGGVDDSTTGDNGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YND9</accession>
    <name>A0A8I6YND9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123407600</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044956699.1">
        <property type="nucleotide sequence ID" value="XM_045100764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YND9"/>
    <dbReference type="SMR" id="A0A8I6YND9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0684610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0684610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0684610"/>
    </dbReference>
    <dbReference type="GeneID" id="123407600"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0568370.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0568370.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0568370"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0684610.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0684610.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0684610"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407600"/>
    <dbReference type="OMA" id="EMIASWL"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF317">
        <property type="entry name" value="S-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="143"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0684610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="430" mass="48467" checksum="F003A6B3DF7FF1FE" modified="2022-05-25" version="1">MYVPRPSHSDAGEQPRVYQVWRGNNEFFLQGRFIFGPDARSIFLTMSLIFAPVVVFCVFVARHLINDFPDHCGISVMVVVVVFTIYDLMLLILTSGRDPGIIPRNTHPPEPESIDGSNYIRGQTPLRLPRTKDVVVNGISVKVKYCDTCLLYRPPRCSHCSICNNCVERFDHHCPWVGQCIGQRNYRFFYMFVFSTMLICLYVFAFCWVYIIKISDAEHLSIWRAMLRTPASVVLIVYCFLCVWFVGGLSVFHLYLMSTNQTTYENFRYRYDRRANPYNRGILNNILEIFCSSIPPSKNNFRARVTVEQARSSSRGFMSPNMGKAVGDLEMGKKPVPWDEPRTTANIGDLEVGLGGMLDEKEGRVAHASPDVSGDELSLEHVEGRAGMHSRNSSWDPRAGTSESVESNSVQTSPSKAADGGGHVTTSDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YNH2</accession>
    <name>A0A8I6YNH2_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="12">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123394906</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="12"/>
    <dbReference type="RefSeq" id="XP_044945713.1">
        <property type="nucleotide sequence ID" value="XM_045089778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6YNH2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529430"/>
    </dbReference>
    <dbReference type="GeneID" id="123394906"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.5HG0440410.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.5HG0440410.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.5HG0440410"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.5HG0529430.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.5HG0529430.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.5HG0529430"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123394906"/>
    <dbReference type="OrthoDB" id="258806at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 5H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
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    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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            <begin position="38"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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            <end position="393"/>
        </location>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0529430.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
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    </evidence>
    <sequence length="459" mass="50792" checksum="2D22DEF6A80DB337" modified="2022-05-25" version="1">MDLPPANGVPTPELPPPPPGRCHFWLPNKRRHCANTPIPSSQYCGNHSPASSSDSRRRVPCPVDPSHTVFEENLEAHVGKCPFRKQADALAAQPYYSKGINSGGGEAGGAVVTSAAKRASVHKLSEEEFWGLVVKIRSAHTAAAVEMRESYIAPDACDKWMKGQVDRKVPYQEKHVVQQVSIVGNMETFGLLPRGGAEDATDEIAVKNPPAAVEFGAGRGYLTQMLADCYGIKNIFLVERRAYKLKADRSLRQNEGVKLERLRIDIEDLNLHGVQALNGGQYLAIGKHLCGPATDMTIMCCLHEQHNHTEEKGHDKHHLRGLALATCCHHLCQWKHYANKSFFSGLGIAEEEFHAMTWFSSWAVDGDHSSQDSLEAEDLSSEGRNRETEKPDPEIIGVERIIRSIPGVERSALGFMCKDIIDTGRLLWLRSKCLDADLVRYVPSDISPENHLLIAKCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6YSH1</accession>
    <name>A0A8I6YSH1_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC123410939</name>
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        <lineage>
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            <taxon>Hordeum</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">cv. Morex</strain>
        </source>
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    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0727000.1.CDS1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        </submittedName>
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                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
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                <person name="Stein N."/>
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            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mascher M."/>
                <person name="Fiebig A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
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    </dbReference>
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    </feature>
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            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0719810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="196" mass="23192" checksum="82B2CB2FEF74D0C8" modified="2022-05-25" version="1">MSLPCSDQSIRPKKMQSASLPRGVEAVRCWCGDLCKVKEVTDFSDWLGMKIFMCANYESDPPESISAYLRPPSPPPLCMYYRWIDTEMPDWAVTEIRERGRRAWASLDLEERREKAEAEEKAEQKKEWEDYCVEQRAFIDEMKRKNQEEKLWLEEVYRQREQARETERERKRERAHAAKAAEEAGDGKGKYPRCTQ</sequence>
</entry>
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        <recommendedName>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044956531.1">
        <property type="nucleotide sequence ID" value="XM_045100596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z0T3"/>
    <dbReference type="SMR" id="A0A8I6Z0T3"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0664470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0664470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0664470"/>
    </dbReference>
    <dbReference type="GeneID" id="123407463"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0551220.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0551220.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0551220"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0664470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0664470.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0664470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123407463"/>
    <dbReference type="OrthoDB" id="6415022at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000207">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046879">
        <property type="entry name" value="KANL3/Tex30_Abhydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026555">
        <property type="entry name" value="NSL3/Tex30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136">
        <property type="entry name" value="TESTIS DEVELOPMENT PROTEIN PRTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136:SF11">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20408">
        <property type="entry name" value="Abhydrolase_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANL3/Tex30 alpha/beta hydrolase-like" evidence="2">
        <location>
            <begin position="30"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0664470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="234" mass="25711" checksum="D4C872BE561F6777" modified="2022-05-25" version="1">MARKRRRADQRTTAPSPPPPLRTKLTHRQPVVVFAHGAGAPSSSDWMTHWKEMVKDALDAIEVVTFDYPYMSGGKRRPPPKADKLVDHHLSVVKNAVAEHPGHPLVLMGKSMGSRVSCMVASSDGICASAVICLGYPLKGAKGVMRDEILLKLIVPTMFVQGNKDGLCPLNKLELVRKKMTCKNELHVVDGGDHSFKISQKYQKSAGINQHDIELEAVKAIEQFVQNSIAESLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1I5</accession>
    <name>A0A8I6Z1I5_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123408180</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044957283.1">
        <property type="nucleotide sequence ID" value="XM_045101348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1I5"/>
    <dbReference type="SMR" id="A0A8I6Z1I5"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653470"/>
    </dbReference>
    <dbReference type="GeneID" id="123408180"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0542170.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0542170.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0542170"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0653470.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0653470.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0653470"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123408180"/>
    <dbReference type="OMA" id="KRMMKMN"/>
    <dbReference type="OrthoDB" id="637156at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF21">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0653470.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="101" mass="11224" checksum="FEB840289C1D5706" modified="2022-05-25" version="1">MGKRKSTSAKTAPRKKAEKLDTTFCCPFCNHPGSIDCKIDLKHMVAEASCSACLESYSTTAHALTEPVDVYAEWIDECEKANADRDGDDDHPRNDDDEHYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z1W9</accession>
    <name>A0A8I6Z1W9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123411082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044959936.1">
        <property type="nucleotide sequence ID" value="XM_045104001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z1W9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657420"/>
    </dbReference>
    <dbReference type="GeneID" id="123411082"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.7HG0545600.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.7HG0545600.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.7HG0545600"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0657420.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0657420.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0657420"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123411082"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF337">
        <property type="entry name" value="OS04G0109900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035263416" description="Exostosin GT47 domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="96"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0657420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="451" mass="50604" checksum="88620059F5750B6E" modified="2022-05-25" version="1">MAAAAARLLAVALSAAILVVVVGGRGVFPVSGVERPSVERELDAARAAIRRAARRHGNNASSAPGTWFRGDDVEYALLARVYRNPAAFHRSYVEMERRFKVYVYEEGEPPILHTGPCKDIYTIEGRFIEQLELLAPPAPGVRTRDADRAHAFFLPFSVAQMMQFAYRQLSYDRGPLLSLVGDYVRVVASRHPFWNRSAGADHFMLSCHDWGPDASKGDPELYANGIRALCNANTSEGFRPGKDVSIPEINLYDGDTPRQLLGPSPGLSARPYLAFFAGGRHGHVRDLLLRHWKGRDPATFPVYEYDLPSTTGGNSSGSHNRRGRDRQSDYFAYMHRSRFCLCPSGHEVASPRVVEAIHAECVPVLVSEGYAPPFADVLRWESFSVSVPVVDIPRLKEVLEGIPTAEVERLREGVRLVKRHFTLRQPPERLDMFHMILHSVWLRRLNFRLDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z4L2</accession>
    <name>A0A8I6Z4L2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
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            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z748"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0725660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0725660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0725660"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0725660.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0725660.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0725660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF8">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="3">
        <location>
            <begin position="37"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="4">
        <location>
            <begin position="318"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0725660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="63167" checksum="CCC44632E7F82A1F" modified="2022-05-25" version="1">MLVSYVDNLPPCPEGSHCSNLVGMEVFDGGGGAGGANNWRSQIREEIRESRISVLSEVLMQVLSPDEPETLTDLLSVAVRLESMIFVVAVNEVDYNYKLYKRMDHLEDILSDNHFEDISSDDHFEDISSDDHFEDIPSDDQMFGPSTATLLRGIFSDRQSQSIQVQSNLQRVLSSGNTKATVPIGNEEKPCCDDNDRISVLHESILHYIMSFMPAQDVVHTSMLFRRSHPLWTSAPCLDIDIDHFDMDRVKFNKFAKGLFEGLFEWRGDANTLDTLHLHSFAIDAAKYWINDAINVHKVKSIDFTENTRWEAFYLDPKAINFRSLYLRSMKLTNVIIVTSVFDELSRECPSLENLQLANSTLCCPEISSSSLETLEISSSSLKTLEIINCLVLNHLLIRTRKLVSLCFKDTRCRCTPKYIVRTTSAVILCDLSNAKSIELQAPVRHVAFDRMASRRLMFISLTSLILGEWCLSNKCAPLACFLKHSPMLENLDLKLKFDCEEQKQPLRTAEGISFQAERLKKITIHCAEKEDRLPVLKAILLANARSVKHINVKTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I6Z7E1</accession>
    <name>A0A8I6Z7E1_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I6Z7E1"/>
    <dbReference type="SMR" id="A0A8I6Z7E1"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0728300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0728300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0728300"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.7HG0728300.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.7HG0728300.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.7HG0728300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 7H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="4">
        <location>
            <begin position="43"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.7HG0728300.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="223" mass="23111" checksum="5EA2BC251EC2405F" modified="2022-05-25" version="1">MHARLVPQTPTDVSHPHTTHTPDFLRLSSAAGLLPAASGAVSDVVVRVLDTGIYPLNRGSFKPAGDGLGPPPSSFSGGCVSAAAFNASAYCNSKLIGAKFFYKGYDAGLGHPINETLESKSPLDTEGYGTHTASTAAGSPVDGAGIYNYAHGRALGMAPTVRIAAYKICWKSGCYDSDILATFDEIPDGVMAHRHGSPPPPLRPPSCSGELPLSPTPILSHIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B1F8</accession>
    <name>A0A8I7B1F8_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC123416050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044962710.1">
        <property type="nucleotide sequence ID" value="XM_045106775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B1F8"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076850"/>
    </dbReference>
    <dbReference type="GeneID" id="123416050"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.1HG0076850.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.1HG0076850.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.1HG0076850"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123416050"/>
    <dbReference type="OrthoDB" id="675864at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 1H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265">
        <property type="entry name" value="AVR9/CF-9 RAPIDLY ELICITED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265:SF9">
        <property type="entry name" value="OS05G0520600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.1HG0076850.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="281" mass="31313" checksum="AA31A88F7A1DCA9A" modified="2022-05-25" version="1">MDMSADQYHYQHEGMEEDGVGRGEQVPAPAAVAAKKLWRMVRAVHLVLVRGLGKHQPKIAALGVNLHHLLSSSKRHHHHHLGSARGQDPAFTTYLAAALSCQSMDPGAAVHPYPRGRGAHGGRGGASSLSCRSMDPGAAVCQQYQYRPRDVEFSCSTTPLHRRRRGAHRHRSQSQHGDLPEHTSAPAVKTLFELMDDDMEEEDVDVEGGTVPWAAGRGPAPRQVRITDSPFQAREEDDEEGRKGVVDRRADEFIVWFHDQLRMQQQQQRPAARDRATRCVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B2U9</accession>
    <name>A0A8I7B2U9_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome endopeptidase complex</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8I7B2U9"/>
    <dbReference type="SMR" id="A0A8I7B2U9"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0153140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0153140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0153140"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0126050.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0126050.1.CDS.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0126050"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0153140.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0153140.1.CDS1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0153140"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0153140.1.CDS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14796" checksum="F3F762A8C7FB1E89" modified="2022-05-25" version="1">MRKIASLDMHVALACTGLKADTRVLINRSRVECQSHRLTIEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYMDKPALYQTDPSGTFSARKANTTGRNSNSMREFLEKNYKDTSGKETIKLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B4X2</accession>
    <name>A0A8I7B4X2_HORVV</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC123442890</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
            <location evidence="12">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the plant 'ANKYRIN-BTB/POZ' family. 'NPR1-like' subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044975002.1">
        <property type="nucleotide sequence ID" value="XM_045119067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B4X2"/>
    <dbReference type="SMR" id="A0A8I7B4X2"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236240"/>
    </dbReference>
    <dbReference type="GeneID" id="123442890"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.3HG0196030.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.3HG0196030.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.3HG0196030"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.3HG0236240.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.3HG0236240.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.3HG0236240"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123442890"/>
    <dbReference type="OrthoDB" id="71307at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 3H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000031">
        <property type="term" value="P:regulation of salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080027">
        <property type="term" value="P:response to herbivore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009862">
        <property type="term" value="P:systemic acquired resistance, salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18310">
        <property type="entry name" value="BTB_POZ_NPR_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000239">
        <property type="entry name" value="BTB/POZ domain and ankyrin repeat-containing protein NPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044292">
        <property type="entry name" value="NPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021094">
        <property type="entry name" value="NPR1/NIM1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024228">
        <property type="entry name" value="NPR_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057250">
        <property type="entry name" value="Znf_C2HC_NPR-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46475:SF1">
        <property type="entry name" value="REGULATORY PROTEIN NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46475">
        <property type="entry name" value="REGULATORY PROTEIN NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11900">
        <property type="entry name" value="DUF3420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12313">
        <property type="entry name" value="NPR1_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52046">
        <property type="entry name" value="ZF_C2HC_NPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="17">
        <location>
            <begin position="53"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC NPR-type" evidence="18">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.3HG0236240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="576" mass="63285" checksum="B24A092B5309C90D" modified="2022-05-25" version="1">MEAPSSHVTTSFSDCDSVSMEDAAPDADVEALRRLSDNLAAAFRSPDDFAFLADARFAVPGAPDLCVHRCVLSARSPFLRALFKRRAAAAGSAGGAEGDRVELRELLGGEVEVGYEALRLVLDYLYSGRVCDLPKTACACVDEGGCAHVGCHPAVSFMAQVLFAASTFQVGELASLFQRHLLDLLDKVEADNLPLVLSVANLCNKSCVKLFERCLERVVRSDLDMITLDKALPLDVIKQIIDSRITLGLASPEDNGFPNKHVRRILSALDSDDVELVRLLLKEGQTNLDDAFALHYAVEHCDSKITTELLDIALADVNLRNPRGYTVLHIAARRRDPKIVVSLLTKGARPSDFTFDGRKAVQIAKRLTKHGDYFGNTEEGKPSPNDKLCIEILEEAERRDPQLGEASVSLALAGDCLRGKLLYLENRVALARIMFPIEARVAMDIAQVDGTLEFTLGSCTNPPPEITTVDLNDTPFKMKDEHLARMRALSKTVELGKRFFPRCSNVLDKIMDDEPELASLGRDASSERKRRFHDLQDTLLKAFSEDKEEFARSATLSASSSSTPTVARNLTGRPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B809</accession>
    <name>A0A8I7B809_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Galactokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123427669</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_044967698.1">
        <property type="nucleotide sequence ID" value="XM_045111763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B809"/>
    <dbReference type="SMR" id="A0A8I7B809"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0192250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0192250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0192250"/>
    </dbReference>
    <dbReference type="GeneID" id="123427669"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0159340.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0159340.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0159340"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0192250.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0192250.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0192250"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123427669"/>
    <dbReference type="OrthoDB" id="275179at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047912">
        <property type="term" value="F:galacturonokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046835">
        <property type="term" value="P:carbohydrate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046396">
        <property type="term" value="P:D-galacturonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000061">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000001">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF6">
        <property type="entry name" value="GALACTURONOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="8">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="9">
        <location>
            <begin position="323"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0192250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="46939" checksum="3F2A315F5F2770AB" modified="2022-05-25" version="1">MVAPAGGGSPSRWPSEEELDIVRKNVVEISGRDAREVRVVACPYRICPLGAHIDHQGGIVTAMTINYGVLLGFVPSDDAEVVLQSGQFKGVTRFRVDDLQKPIEIRGNITWESYARGAVYALQNSGHDLRKGIIGYISGVNGLDSSGLSSSAAVGIAYLLALENVNDLVLSPVDNIQLDKSIENKYLGLENGILDPSAILLSRYGYLTFMDCKTALPSYVYFSELSKSQQPQGRLPFKILLAFSGLQHNLPKSRGYNMRVFECKEAARALLCALGCEDASVLCNVDPGVYEAQKCILDENLARRAEHYFSEMKRVVKGRDAWARGDLQELGQLVSASGRSSIVNYECGSKEMIQLYEILLKAPGVLGARFSGAGFRGCCLAIVESDRAEEAAAYVGTEYEKAQPELVSKIPADRRVLVCEPGDSARVILQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7B814</accession>
    <name>A0A8I7B814_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123430755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044970530.1">
        <property type="nucleotide sequence ID" value="XM_045114595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8I7B814"/>
    <dbReference type="SMR" id="A0A8I7B814"/>
    <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0192910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0192910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0192910"/>
    </dbReference>
    <dbReference type="GeneID" id="123430755"/>
    <dbReference type="Gramene" id="HORVU.MOREX.r2.2HG0159880.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r2.2HG0159880.1"/>
        <property type="gene ID" value="HORVU.MOREX.r2.2HG0159880"/>
    </dbReference>
    <dbReference type="Gramene" id="HORVU.MOREX.r3.2HG0192910.1">
        <property type="protein sequence ID" value="HORVU.MOREX.r3.2HG0192910.1"/>
        <property type="gene ID" value="HORVU.MOREX.r3.2HG0192910"/>
    </dbReference>
    <dbReference type="KEGG" id="hvg:123430755"/>
    <dbReference type="OMA" id="WNPKHIL"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000011116">
        <property type="component" value="Chromosome 2H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008264">
        <property type="entry name" value="Beta_glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00737">
        <property type="entry name" value="GLHYDRLASE16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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        </location>
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        </location>
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        <source>
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        <source>
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            <dbReference type="PROSITE" id="PS51762"/>
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            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.2HG0192910.1"/>
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    <sequence length="296" mass="34028" checksum="292264F43D0938D7" modified="2022-05-25" version="1">MAPALPCRSRLRLLCVALAFLLALDMGMADMYKDIEIIWSADHTFYFMDGEREALALSLDYNRGSAFKSKEMYHFVRIDIDIKLVEGNSAGTVCTVYTISEGPWEVHDEIDLEFLGNSTGEPYTLHTNIFANGVGGREQQFKLWFDPSSEYHTYSIVWNPKRITIEVDGVTVRTFDNNQDQGVPFPSYQRQRVYGSFWSAEDWATQGGRVKTDWKLAPFISYYSNYNVTWCQPSPGVLWCGTEPAESTHFNLSPKARADLQWVRDMGYVIYDYCTDRSNRYNDTTRPKECSLPPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAH3</accession>
    <name>A0A8I7BAH3_HORVV</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
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            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
            <authorList>
                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">cv. Morex</strain>
        </source>
    </reference>
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        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">subsp. vulgare</strain>
        </source>
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    <dbReference type="PANTHER" id="PTHR35163">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="HORVU.MOREX.r3.5HG0533030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000011116"/>
        </source>
    </evidence>
    <sequence length="209" mass="24524" checksum="71A39D78B273EC14" modified="2022-05-25" version="1">MMHHQRPKKMVAFEGSLSGSRFLGCPVQQDEGVNCGVVQWVDAPWPEILQRCLARLWEMYQEQNFGRVKDQQAHDKEVVKLKKEIDFLSNSYSQLVEDVSKLFDYQDGKMSHDMDYTSEAINKKKKELEDQARIEISMEKMKLAKEQRCILQSQADIIQNMRKAMKEVQVDRDLLKEEKKKLEYLIVDLLKAGHCTKEKMERIKAIVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8I7BAW7</accession>
    <name>A0A8I7BAW7_HORVV</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123445292</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hordeum vulgare subsp. vulgare</name>
        <name type="common">Domesticated barley</name>
        <dbReference type="NCBI Taxonomy" id="112509"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Triticodae</taxon>
            <taxon>Triticeae</taxon>
            <taxon>Hordeinae</taxon>
            <taxon>Hordeum</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Nature" volume="491" first="711" last="716">
            <title>A physical, genetic and functional sequence assembly of the barley genome.</title>
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                <consortium name="The International Barley Genome Sequencing Consortium"/>
                <person name="Mayer K.F."/>
                <person name="Waugh R."/>
                <person name="Brown J.W."/>
                <person name="Schulman A."/>
                <person name="Langridge P."/>
                <person name="Platzer M."/>
                <person name="Fincher G.B."/>
                <person name="Muehlbauer G.J."/>
                <person name="Sato K."/>
                <person name="Close T.J."/>
                <person name="Wise R.P."/>
                <person name="Stein N."/>
            </authorList>
            <dbReference type="PubMed" id="23075845"/>
            <dbReference type="DOI" id="10.1038/nature11543"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Scholz U."/>
                <person name="Mascher M."/>
                <person name="Fiebig A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">cv. Morex</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2022-01" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">subsp. vulgare</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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